<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="http://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
 xsi:schemaLocation="http://uniprot.org/uniprot http://www.uniprot.org/docs/uniprot.xsd">
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPN0</accession>
    <name>U5VPN0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Laminin G sub domain 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_02350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357702.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02350"/>
    <dbReference type="EnsemblBacteria" id="AGZ38759">
        <property type="protein sequence ID" value="AGZ38759"/>
        <property type="gene ID" value="AFR_02350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.475"/>
    <dbReference type="eggNOG" id="COG4886">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_481155_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665199" evidence="2">
        <location>
            <begin position="23"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="LamGL" evidence="3">
        <location>
            <begin position="427"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="566" mass="57216" checksum="5706BB09CC72525B" modified="2014-01-22" version="1">MVKRAMIAGAVLSLLASGAAYGYWGTMGTGSGSAQTGTAAPLTLTPGTPTTRLYPGGSADVAVTVTNPNPYAVRVGRLALDTAQGTGGFAVDAGHAACGVASLAFATQSNGGDGWTIPANGALSLQLAGALTMTTAAANACQGATFSVYLATDRDAYAAVRNTSGLVSYWRLGADPDVADNFTGTPATALPAHTGTVATSWTTFAGTDPVLSAANRVRRSVPGWSLVHSGTAPASADYTVEADVYAATLVAGDSIGVVGRLNTSTSSYYSARYRMSDQSWHLYRTAGGTNTSLASAGATLSAGQTYRVRLDLAGTTLTLSIDGMVVATATDATLAATGRAGLQLGENGVNAAVSDSTGLHADNFRVFRNSGSAASDSAGANSGAYLGSVVQNEPGALAGDLNAAVVLNGSTGAMRVAPATGLPVGAASRSVEIWFKRSGTTDAALFSYGSASAAKLFAARLTSANNLRVWGFALDRDFTLPYPTSDNVWHHVVVTYDGGVLRVYLDGTGLTPLTATLDTTVDGSGFTVGSATGAYFFGGSLDEVAVYSQVLSPATITDHYRAGRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPY8</accession>
    <name>U5VPY8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">YusP-like MFS-type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02900</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357812.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02900"/>
    <dbReference type="EnsemblBacteria" id="AGZ38869">
        <property type="protein sequence ID" value="AGZ38869"/>
        <property type="gene ID" value="AFR_02900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.583"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_11"/>
    <dbReference type="OrthoDB" id="1108236at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="13"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="589" mass="61567" checksum="0C0069013A013BC4" modified="2014-01-22" version="1">MLTVDHPRYKWIALSNTTLGTLLATINSSIVLISLPAIFKGIHLNALEPGNVSYLLWMLMGYMLVTAVLVVTLGRLGDMYGRVKIYNLGFAIFTVTSVILSLDPFDGGGGALWLIGWRVLQAVGGAMLMANSAAIITDAFPAKQRGMALGVNIVSALAGSFIGLVAGGVLAEWDWRSVFWVNIPLGVLGTVWAYRSLHDSGVRRKAKIDWWGNGTFAAGLTAVLAAITYGIQPYGGHTMGWTNPWVLAGLIGGGLVLVAFGVIESRVAEPMFPLALFRNVAFTSGNAANLLASIARGGLQFMLIIWLQGIWLPLHGYDYESTPLWAGIYLVPMTIGFLAAGPLAGYLSDKFGPRPFASGGLLVMAASFVGLLLPGDFNYAVFATLIFINGLGGGLFAAPNTSLIMSSVPANMRGAASGMRATFQNAGQVLSIGVFFSLMVAGLASSLPSTLSSGLTAQGVPAATAGQIAQLPPVGTLFAAFLGYNPIAELLGPQVLGALPAGNAATLTGLEFFPRLISGPFHDGLVIVFWLAIAMTVVGALVSLLRGPKPTETSDDPVAHEAELVSELAEAAPLTPAARIAGPLDPVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ14</accession>
    <name>U5VQ14_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357837.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03025"/>
    <dbReference type="EnsemblBacteria" id="AGZ38894">
        <property type="protein sequence ID" value="AGZ38894"/>
        <property type="gene ID" value="AFR_03025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.608"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_697626_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013431">
        <property type="entry name" value="Delta_60_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17164">
        <property type="entry name" value="DUF5122"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665132" evidence="1">
        <location>
            <begin position="32"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="39185" checksum="3FF61279D0624B9B" modified="2014-01-22" version="1">MRFLRLVRKSLIVGAVAALTLTTTGVSPAQAVSSSPAKAPLFNGSVYAVAYRGDTVYVGGSFTSVGWGGRTYERQRLAAFDVSSGSLLSWSPSADATVRALAINGTSVYAGGDFHRISGTRRDALARLDAFSGALGTFSHTVTGAPYALTAANGRLYVGGSFGSVNGNTRRNLAAFSLSDGSLDGRWRPSADDSVHALAAAGSRVYAGGAFHKISGVSNTLRVAALSGLTGGVYTSFRANAPAQVNSLTTDAGGVYVATGGQGGKAIAYSSAGKVRWTRVFDGDAVAITSLAGTIYVGGHFDRACLTNRNGAQGVCTDGSVKRVKLASITSRGLLTGWSPAANGVIGVRVLTADRTRNAIAAGGDFTMINDENRKRLAFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQC0</accession>
    <name>U5VQC0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_04170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 4">Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the UPF0677 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358068.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04170"/>
    <dbReference type="EnsemblBacteria" id="AGZ39123">
        <property type="protein sequence ID" value="AGZ39123"/>
        <property type="gene ID" value="AFR_04170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.842"/>
    <dbReference type="eggNOG" id="COG3315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056160_3_0_11"/>
    <dbReference type="OrthoDB" id="847145at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011610">
        <property type="entry name" value="CHP00027_methylltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00027">
        <property type="entry name" value="mthyl_TIGR00027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="29360" checksum="D99AB62B9E9061E5" modified="2014-01-22" version="1">METGQPSRTAFSAARYRAAHQLIEGGEIFSDPLAVRILGVPPDAEEAPGRRAMRLFIAARTRFAEDALAEAVRNGTRQLVVLGAGLDTFAYRNPWPELHVFEVDHPDTQAFKRQRLAEAGIAVPEGVTYVPVDFEREKLTLPVGPQAFFLWLGVVPYLTRTGFDETLGLIAATPRSEVVFDYAMPPSSMATPEKQAALEVRAARVARLGEPWQTYFLPDDLAAELRKLGFDQLEDLGPSELAARWFGRPDVPKDTPGGHVVHARQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQR8</accession>
    <name>U5VQR8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative NADH dehydrogenase I chain L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_04265</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358087.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04265"/>
    <dbReference type="EnsemblBacteria" id="AGZ39142">
        <property type="protein sequence ID" value="AGZ39142"/>
        <property type="gene ID" value="AFR_04265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.861"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_6_3_11"/>
    <dbReference type="OrthoDB" id="1274678at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018393">
        <property type="entry name" value="NADHpl_OxRdtase_5_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="PTHR42829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01974">
        <property type="entry name" value="NDH_I_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="509"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_N" evidence="8">
        <location>
            <begin position="71"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="7">
        <location>
            <begin position="135"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="633" mass="65901" checksum="C1A7A127DDDBCF87" modified="2014-01-22" version="1">MIAEVFGTALFGVPVLLGLAGLLLPAGDGGRPRRRLAAGLGIAGAAIALAEVVFLLVAGSDPVEFGAEVVRFGDLPVTFGVSLGPAALYVALAVTVVALLVQIYSVGYLHDDPRYAPYAAQISIFTGAMLMVVAANDLILLLVGWEIMGACSYLLIGHDRRLPEAPRAAVKAFLVTRVGDVGFLLGIALLGVSAGSFRIADVLAAPPEHLTIALLLILAGVAGKSAQFPLHTWLPDAMAGPTPISALIHAATMVAAGVYVVFRLFPLFARSPGALAVLGVMAAITLLLGAFSATAQDDLKRVLAWSTVSQIGYMTGALAVGAPAAALFHLLTHAAFKALLFLAAGGVIHAVGTNLMSRMGGLRTHMPVTFWSFVIGLGALAGLPPLAGFWSKENVLVAAAHATEGHGPAPAWVGWVVWLAGLLGVAITAWYATRLLLRTFFGHPRSRTEQWHVGFDDARYERGDGAPHDPPALIRWPVLLLTVPAALLGLIAFVPAFRSALELEAPHLSVSVVLPMLLLALGAGSSWWLWHTAPGADPALALGRLRPFFAAGFRIDDLQDRFLVRPVRAFATALRIVDERVVDAAVEGTGTSATRFGTVLSTAHRAALPRAAVAVLAGALVLGVVAAIYGAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRL5</accession>
    <name>U5VRL5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_06470</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06470"/>
    <dbReference type="EnsemblBacteria" id="AGZ39583">
        <property type="protein sequence ID" value="AGZ39583"/>
        <property type="gene ID" value="AFR_06470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1312"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="205"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="262"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="520" mass="53705" checksum="F036A03040D89499" modified="2014-01-22" version="1">MSQKLFASFGVLSVLVITMGVTGGIELNSANNRLEDMYQNNLQSIARLGEVRADVQQATSLSAKLILRSPLADVSNVQMAIKRLDADIDSTWTAYTKSPAAGTQTDRAAFAAGLAEYRKVRDQQLVPAAQANSLSTYLGVQNNFIDPLTSKITVALNNLAGIEDKAAQQQMADARSNATMARILTIALMGAALAFAVVIAIAVSRAIARPLGETVKVLEALAEGRLDQRLKVRGRDEVGRMAEALNTALDRLTAALRDIGTNVTTLASSSEDLTAVAGRMNSSAARSASRAHAVSTASDEISTNIATVSAGADEIGSSITEIARSTSSAADVAGQAVRISGEAGAILQKLGESSAEIVSVVKIITGIAEQTNLLALNATIEAARAGDAGKGFAVVAGEVKELAQETARATEDIRTRVGAIQTDSSAAVAAIAEIGAVIDQINATQTAIAAAVEEQTATTNEMGRNVGEVAAGSHEISSNVAEVAEAAAETTSAAANTAAAADELARVAHELQQSLAMFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRT2</accession>
    <name>U5VRT2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_06895</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O = 2,5,6-triamino-4-hydroxypyrimidine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23436"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:137796"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-6-(5-phospho-D-ribosylamino)uracil + H2O = 5,6-diaminouracil + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:55020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:46252"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06895"/>
    <dbReference type="EnsemblBacteria" id="AGZ39668">
        <property type="protein sequence ID" value="AGZ39668"/>
        <property type="gene ID" value="AFR_06895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1408"/>
    <dbReference type="eggNOG" id="COG3236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084247_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd15457">
        <property type="entry name" value="NADAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012816">
        <property type="entry name" value="NADAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037238">
        <property type="entry name" value="YbiA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08719">
        <property type="entry name" value="NADAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143990">
        <property type="entry name" value="SSF143990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02464">
        <property type="entry name" value="ribofla_fusion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADAR" evidence="3">
        <location>
            <begin position="31"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="184" mass="20770" checksum="48E0BD813E732E9C" modified="2014-01-22" version="1">MNEMPGTLDEVIRLAGAGRPMKYVFFWGHRPQRDGSIGTGCLSQWWPSVFTVDGVRFASAEHYMMWRKALLFGDEESAGRIVAASHPRQAKMLGRRVRGFDEQTLVDHRWSIVVDASVAKFGQRPELRDFLLGTSKRILVEASPTDRIWGIGLSATDDRVHDPAQWRGLNLLGFALMEARTQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRY9</accession>
    <name>U5VRY9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359130.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06550"/>
    <dbReference type="EnsemblBacteria" id="AGZ39599">
        <property type="protein sequence ID" value="AGZ39599"/>
        <property type="gene ID" value="AFR_06550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1328"/>
    <dbReference type="eggNOG" id="ENOG5030HZT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191581_0_0_11"/>
    <dbReference type="OrthoDB" id="1920995at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="82" mass="9083" checksum="B06336D2D8A01D62" modified="2014-01-22" version="1">MTPLLAATSLIAGYALLCWVLPFGRCWRCRKTGSHPRLITRKLTTCRRCKGAGLRLRHGRAAFNYLARIHADAASAKNSGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSJ1</accession>
    <name>U5VSJ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_01510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01510"/>
    <dbReference type="EnsemblBacteria" id="AGZ38591">
        <property type="protein sequence ID" value="AGZ38591"/>
        <property type="gene ID" value="AFR_01510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.303"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_43_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="179" mass="18988" checksum="01D86F7EA44A416A" modified="2014-01-22" version="1">MVAVALTLVDEKGLEGLSLAAVADRAGVAAPSLYKHVGSLADLRDLMALRVLRDVTATLSTVVMGRSRDDAVAALMQAYRSYVVTHPGRYALIPFDPLRHEALAEASGALLGVFFAVLRGYGLDESETTHATRRLRVTAHGFADIEANGGFGMGEDVDTTFDQVVAMVLASLPRTDVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSJ2</accession>
    <name>U5VSJ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hemolysin-type calcium-binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359494.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08370"/>
    <dbReference type="EnsemblBacteria" id="AGZ39963">
        <property type="protein sequence ID" value="AGZ39963"/>
        <property type="gene ID" value="AFR_08370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1703"/>
    <dbReference type="eggNOG" id="COG2931">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302734_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.150.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018511">
        <property type="entry name" value="Hemolysin-typ_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001343">
        <property type="entry name" value="Hemolysn_Ca-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011049">
        <property type="entry name" value="Serralysin-like_metalloprot_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00353">
        <property type="entry name" value="HemolysinCabind"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51120">
        <property type="entry name" value="SSF51120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00330">
        <property type="entry name" value="HEMOLYSIN_CALCIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665702" evidence="2">
        <location>
            <begin position="26"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="20106" checksum="FF309BE6413E9CF5" modified="2014-01-22" version="1">MSRRLLATVVLALAASAALTTPAHADEVPCGSGGPGVVWDFSGAGAGLVINGTPGDDIITGTPFADVIRGGGGHDIINGGQGNDQLRGDGIPGQLPGKDQVNGQENDDTLNGDECADTVRGGDGADIATGGSQNDLVNGGAGGDVLSGGDGNDEVNGNDGGDTVSGGIGDDTLSGGNGADGLDGQPGSDSGDCGGQAGDFATAATELTANC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSU2</accession>
    <name>U5VSU2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02505"/>
    <dbReference type="EnsemblBacteria" id="AGZ38790">
        <property type="protein sequence ID" value="AGZ38790"/>
        <property type="gene ID" value="AFR_02505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02505"/>
    <dbReference type="HOGENOM" id="CLU_2476367_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="87" mass="9889" checksum="1CBC34F894939119" modified="2014-01-22" version="1">MSWAAAPCHSSSRPSSIASTVRRARDWARRLRSWEMRTLTSSWPILMNDGTRAAPKPVARAMAISTTWVKAAMTMLYWVASESRAYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT34</accession>
    <name>U5VT34_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Regulatory protein TetR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08415</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359503.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08415"/>
    <dbReference type="EnsemblBacteria" id="AGZ39972">
        <property type="protein sequence ID" value="AGZ39972"/>
        <property type="gene ID" value="AFR_08415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1713"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_5_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003012">
        <property type="entry name" value="Tet_transcr_reg_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00400">
        <property type="entry name" value="TETREPRESSOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="6">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="24433" checksum="7E8B2681A2C122AA" modified="2014-01-22" version="1">MEAKAVREPLSRERALAAAVTLVDAEGLKALTMRRLAADLGVEAMSLYYHLPGKNGLLDGLADTVIDEITTAVAATAVPDADWRTSLRQRFLAAREVMLRHPWAPGLLGSRPTIPSGLFAYYDAILATLVGGGLSYRLAHRALHAFGSLALGFAQEVFSPAPAGGSMDVGATEADLSALAATLPHLTAMVEAEVHAVEDPTLGWCDSQVEFTFTVDLLLDGLARAQERQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT50</accession>
    <name>U5VT50_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_02685</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357769.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02685"/>
    <dbReference type="EnsemblBacteria" id="AGZ38826">
        <property type="protein sequence ID" value="AGZ38826"/>
        <property type="gene ID" value="AFR_02685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.540"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_0_3_11"/>
    <dbReference type="OrthoDB" id="1722208at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="374" mass="40602" checksum="302CEE995B3F25C2" modified="2014-01-22" version="1">MLPYGRQNVTEADIEAVSTALRSDWLTTGPRVDLFENDLERIAGAPCVAVINGTAALHTAYAAAGVGPGDEVVTTPMTFVATASTAAMLGATVVFADVEDDTANLDPAAAEAAVTSRTRAVTAVDYAGHPAEYDQLRKVTDQAGAVLIADAAHAIGSTYHGRPVGTLADLTTFSFFPTKNLTTGEGGAVAGTVPELLDRARRFRTVGITRDKAMLRHPDEGGWHQEVPEFGLNYRLPDVLCALGSSQLRRLSAFKARRTALAERYDELLAGVPGLRLPVRRAWADPMRHLYPVRIQDGRRREVYDRMRSAGIGVQVNYIPAYWHPAFEDLGYRRGMCPVAEGFYAEQLSLPLFVDLSDADQDRVVDTLRTILES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTB6</accession>
    <name>U5VTB6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08740</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08740"/>
    <dbReference type="EnsemblBacteria" id="AGZ40037">
        <property type="protein sequence ID" value="AGZ40037"/>
        <property type="gene ID" value="AFR_08740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1778"/>
    <dbReference type="eggNOG" id="COG3325">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027195_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="6">
        <location>
            <begin position="22"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="5">
        <location>
            <begin position="312"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="443" mass="46047" checksum="AD52A22B1A28C878" modified="2014-01-22" version="1">MVVTSAVAFAGTSEAAVLPNNFKSVGYMPSWAGSVNSIQYSKLTHINYAFVLPNANGTLQGVPDPGKLQSLVSLGHQNNVKVSLALGGWNDGNDSAFEALAGNSGSRTTFVNAVMGLVGQYNLDGIDIDWEYPDPGTSGNNYTALMQQLSTALHGQGKLLTAAVVSEGGTANGVQPAVFGMVDWLNIMAYDGGSPHANYDWSIASVNFWKNRGLPASKAVLGVPFYSRPGYKTYAQIVATNPAFANQDCATISGVNECYNGLPTVRRKTQWAMANAGGMMNWELSQDATGANSLVSAIFTTATGGSTPPTTPPPAGRTGPITGIAGKCVDIAAASSANGTAVQLYDCNGTTAQNWTVASDSTLRALGKCLDIAAAGTANGSLVQIYDCNGTGAQVWQAQSNGTLRNPVSNKCLDASGNSSANGTRLQIWECFAGTNQRWTLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTF6</accession>
    <name>U5VTF6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TGc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_09920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09920"/>
    <dbReference type="EnsemblBacteria" id="AGZ40273">
        <property type="protein sequence ID" value="AGZ40273"/>
        <property type="gene ID" value="AFR_09920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2023"/>
    <dbReference type="eggNOG" id="COG1305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008359_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025403">
        <property type="entry name" value="DUF4129"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021878">
        <property type="entry name" value="TgpA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13559">
        <property type="entry name" value="DUF4129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11992">
        <property type="entry name" value="TgpA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="611"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="TGc" evidence="3">
        <location>
            <begin position="470"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="811" mass="86535" checksum="7606CCC0102DDF21" modified="2014-01-22" version="1">MAAAATLLAAAPLKAIFDSWTWLLECILAVGLIAGAAVLARTLRFPTWAQALSMVSVLLLTLTWMFPSGDELLSLLPSPATFNHFGQLFTDAGNDTRSYGVPVPDRSGLLFIAVLGIGTVAIAVDLLTVVARKPALAGLPMLAIYSVPVAVYVDSVPVLPFIVGATGFLWLLVADNVDRVRRFGRRFTGDGRDVDVWEPSPLAAAGRRLAVIGVAVAVLLPLAVPGLNTGLLNSLTQVGTGNGTGNGSGGTGRINLFASLSGQLNQTQTTNYVRVTTNEPDPFYLRFGVADVITAEAVTNRTPTGRPISRGLDDPRQNNTSGRANYQQYRAEVEITEDFGQTLAPVYSSVIDTDGLDGAWSFDPNTQVVFSNRTTTKGKKYSFDYVRAKFTPAELRTAEALPRDNPLRTQFTAVPDDPKVKTLVANLTKNSNTEYDKVSAIYRYFSRDNGFSYSLATVDPAAGASAISSFLDSKAGYCQQYAAAMAWMVREAGIPARVAFGFTRGSTRDGNAYVLTNRNAHAWTEVYLDGFGWVPFDATPAASVVGSTRSAWATDTDAVETPSPTSTASLAPGADASAAPGAADRPDRDLNDGALDAPGASSDAGISTTTLLTVALIALLLALLTIPALRRLMVRKRRHAATIPKTATVATGPAPPGSREVVVTTEAVQAREDAHAAWDELVDTMVDYRVPVDPTETPRHTAQRLVREADLAGPPADSATLLGRAEERARYARQPLQGGELTAALNQVRKGLAKTATKRTRLAAVLLPPSVLMRWRLSVGDVSARGMGFFSRGRDVLVRFSPRRLLASRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTN0</accession>
    <name>U5VTN0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="3">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_09650</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the class-II DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359883.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09650"/>
    <dbReference type="EnsemblBacteria" id="AGZ40219">
        <property type="protein sequence ID" value="AGZ40219"/>
        <property type="gene ID" value="AFR_09650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1967"/>
    <dbReference type="eggNOG" id="COG3200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026885_0_1_11"/>
    <dbReference type="OrthoDB" id="1371606at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00084"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002480">
        <property type="entry name" value="DAHP_synth_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21337">
        <property type="entry name" value="PTHR21337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01474">
        <property type="entry name" value="DAHP_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01358">
        <property type="entry name" value="DAHP_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="468" mass="51385" checksum="985C206AE58C2B7D" modified="2014-01-22" version="1">MRREWHQLSHPGVGATALQTSRPSTDSAEDEALGLDRWRSLPRVQMPPWEDMDEVASVCQVLDSVPSIVAPYEVDQLRVRLAEVCEGRAFLLQGGDCAETFADNTESHLLANARTLLQMAVVLTYGASMPVVKVARVAGQYTKPRSSAKDSLGLPAYRGDLINSLEANEKARVADPQRMIRAYANSAAAMNMLRAYLAGGLADLAGLHDWNKDFVRASPAGERYEAIAREIDRTLDFIRACGMTEDEALRTVSLYCSHEALALEYDRALTRVSDGKAYGLSGHFLWIGERTRQLDHAHIDFISRIANPIGVKLGPGTSPETAIELCEKLNPDNIPGRLTLISRMGNGKVRDALPPIVEKVTASGAKVVWQCDPMHGNTHESSNGYKTRHFDRVVDEVLGYFEVHRGLGTHPGGIHIELTGEDVTECLGGAQGIEDLDLPDRYETACDPRLNTQQSLELAFLVAEMLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTP3</accession>
    <name>U5VTP3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MglC-like galactoside transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_10395</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360176.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10395"/>
    <dbReference type="EnsemblBacteria" id="AGZ40368">
        <property type="protein sequence ID" value="AGZ40368"/>
        <property type="gene ID" value="AFR_10395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2118"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_3_0_11"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="33415" checksum="0A4F86FD39B38BE4" modified="2014-01-22" version="1">MNFSGLIQDFGPLTITGLAQGAIIALFALGYTLVYGVLRLINFAHSEVFLLGSYACLVVWGFFGLDQNSPTPSVLGVIGLICLGLVAAIVVSGITALGVELVAYRPLRKRNAPPLAFLITAIGASLFISETVGVMTQRNIRGVPPLIQPRDVFVIGNMHVTNLQILIIVLALVMMFVLDRFINRSRLGRGIRAVAQNPDSAALMGVNKSRVISLVFLLGGLMAGIAAVMYDLKIGVTRFDAGFLLGIEAFTAAVLGGIGNLRGALLGGLMLGVLQNYAASLFGTEWLHAASFVLLVLILLVRPTGLLGESLGRARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTV5</accession>
    <name>U5VTV5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_10075</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360050.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10075"/>
    <dbReference type="EnsemblBacteria" id="AGZ40304">
        <property type="protein sequence ID" value="AGZ40304"/>
        <property type="gene ID" value="AFR_10075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2053"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_4_4_11"/>
    <dbReference type="OrthoDB" id="1714699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="4">
        <location>
            <begin position="89"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="308" mass="33266" checksum="CB91779487787D3C" modified="2014-01-22" version="1">MTARSLPVPDGLDGMRLDQAVSRLFGLSRTAAASLVEAGDALVDGYPRPKSDKVSAGAWLEVTLPAPVTAPTFEATPVHGMKIVYSDDDIVVVDKPVGVAAHPSPGWSGPTVIGGLAAMGQNVATSGAAERQGIVHRLDVGTTGLMVVAKSEMAYSALKRAFKEREVDKRYHAIVQGHLDPLRGTIDAPIDRHPTADYRFAVMSDGKPSVTHYDTLEAFRSASLVDVKLETGRTHQIRVHFSAMRHPCVGDITYGADPTLAARLDLHRQWLHAKELGFLHPRTQDEVRFVSDYPEDLGHALDVLRDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUI7</accession>
    <name>U5VUI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360398.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10965"/>
    <dbReference type="EnsemblBacteria" id="AGZ40482">
        <property type="protein sequence ID" value="AGZ40482"/>
        <property type="gene ID" value="AFR_10965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2233"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_1_0_11"/>
    <dbReference type="OrthoDB" id="504558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="2"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="379" mass="40193" checksum="8AFE6816C7E473BB" modified="2014-01-22" version="1">MTNVLISGASVAGPALAYWLHRYGVDVTVVEKSSAIRGGGYPIDVRGSAVDVVERMGVLPELREHHIDTRRITAIDGRGGTVAAFSPGALTGGQAGRDLELPRGDLTSVLYGKTRDDVEYVFGDSIASVTQHDDRVDVTFERGAPRSFDYVVGADGLHSNTRRLVFGPEAEFSHYLGYCFAGFTAPNTGGWSHEAILQNVPGKLAAVYAVGDQPWVHALLAFATTSPSREAVAGTFAGLGGPTPDLLAALSEADDVYFDTVSQIRMPGWVRGRVALVGDAAYAPSFLTGQGTSLALSGAYVLAGELAKGTLPAYEKTLRDHVTRNQGLVSGGTAMVPGTRTQLWMRNQAFKLLPLLARTKLAGRSERRAATSLALPTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUK1</accession>
    <name>U5VUK1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphatidic acid phosphatase type 2 domain-containing protein 1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11770"/>
    <dbReference type="EnsemblBacteria" id="AGZ40643">
        <property type="protein sequence ID" value="AGZ40643"/>
        <property type="gene ID" value="AFR_11770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2397"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072573_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665731" evidence="2">
        <location>
            <begin position="23"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="3">
        <location>
            <begin position="89"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="228" mass="24635" checksum="668AC11AEEF2FE9A" modified="2014-01-22" version="1">MKRLTTVSAALALMVLVPFALLAVLVTGDWSPLHTLDATVTSNLHDWALTHPHLTEANLWWSAIFSPNPLRVAALILAVWLYRRHARRLALWVVTTMTVGGLLGILLKLLIGRHRPDLLDPVARAAGFSFPSGHALNAALTAGVFVLVLLPLARDGKRLLLWTAAAVVTVVTGLTRIVIGVHWTSDVVAGWLLGIAVVAITAAAFPRLRPEPIVEEGLEPDLAEPRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUL0</accession>
    <name>U5VUL0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_11090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360444.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11090"/>
    <dbReference type="EnsemblBacteria" id="AGZ40507">
        <property type="protein sequence ID" value="AGZ40507"/>
        <property type="gene ID" value="AFR_11090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2259"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136818_0_0_11"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="33"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="17687" checksum="174B4DB1D80FD90A" modified="2014-01-22" version="1">MPDRYAHCTFCGARFVPGQPWPRRCGACGETSYLNPTPVAVVLQPVGDGLLVVRRGIAPARDRLALPGGFIEVGETWEQAAVRELSEEVGLTADPDSVRLYDTISAPDSTLLIFGLLPALSSEEDLPTFVTSKEAIGRHVLSGPEPLGFDLHTRVADRWFASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUM9</accession>
    <name>U5VUM9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_11190</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360483.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11190"/>
    <dbReference type="EnsemblBacteria" id="AGZ40527">
        <property type="protein sequence ID" value="AGZ40527"/>
        <property type="gene ID" value="AFR_11190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2279"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_3_11"/>
    <dbReference type="OrthoDB" id="1619224at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="100"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="35750" checksum="2A3E2D5E939CCF49" modified="2014-01-22" version="1">MSVVDSPSAPPAQQPTAPDQADGTRLKRRSATSGGLAPLVLTLPALLLLAGLLGYPLVRMVVLSFQSMGLRQLFGNLPPDNVGFANYVKIFTNQTFYEVVARTVTFTVVSVLLTVVLGLGVALLMRRVAPSIRIVMIIAMMFVWAMPQLVATQTFRWLVDSDFGVLNYLIDLLPGVDFANHSWFADPIQGWTVITSIVVWAGIPFIAITLNAGLTQVPKELLEAATVDGASTWQALLKVTLPILKPLLVIVTTLSVIWNFGLFTQVWGVRNGRPEEDYQVLATWSYSTAFQNNNYGMGSAIAVVTVLLLLGVTAFYMRQMFRIGDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUW3</accession>
    <name>U5VUW3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Allantoin permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_06180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359056.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06180"/>
    <dbReference type="EnsemblBacteria" id="AGZ39525">
        <property type="protein sequence ID" value="AGZ39525"/>
        <property type="gene ID" value="AFR_06180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1255"/>
    <dbReference type="eggNOG" id="COG1953">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021555_0_2_11"/>
    <dbReference type="OrthoDB" id="326558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="PTHR30618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="509" mass="55036" checksum="9961E00BB81567C8" modified="2014-01-22" version="1">MSDTTVAKNSDLIEAAGMPVGSGEIKPGYDPRLTNEDLAPLRKQTWGSYNIFAFWMSDVHSVGGYVTAGSLFALGLSSWQVLVSLLVGITIVYFFCNLVAKPSQLTGVPYPVINRSAFGVLGANVPAIIRGLIAVAWYGIQTYLASAALDVVVLKLFPDLMPYADVNQYGFLGLPLLGWCTYAILWVLQAAVFWTGMETIRKFIDFCGPAVYVVMIVLTGYLIWKAGWGNINLSLGEVKYSGASAIPVMLGAIALVVSYFSGPMLNFGDFSRYGKSFKAVKTGNLLGLPLNFLAFSILVVVTASLTIPVFGELITDPVATVARIDSTFAIVLGALTFTIATIGINIVANFISPAFDFSNVSPQRISWRAGGMIAAVGSVLITPWNLYNNPDVIHYTLETLGAFIGPLFGVLIADFYIVRKQKVSVDDMFTMSPEGRYWYTKGYNPPAIIATAVGAVVAMVPVLWTGGPGMHTTAQYSWFIGMALGFVVYLGLAPRMRVQERSRILDEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUY6</accession>
    <name>U5VUY6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358997.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05885"/>
    <dbReference type="EnsemblBacteria" id="AGZ39466">
        <property type="protein sequence ID" value="AGZ39466"/>
        <property type="gene ID" value="AFR_05885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1195"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059319_2_0_11"/>
    <dbReference type="OrthoDB" id="2027419at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665745" evidence="1">
        <location>
            <begin position="35"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="229" mass="24309" checksum="A8C36276AF46BB96" modified="2014-01-22" version="1">MKRLWSRIGIRLASVCLLATGLVGGIYLGQNASAEPGTAAPDVLQAEAADMQLLKLRQGAHAAARAQQRQAEGNAAERATAEARSAAAKGRKLEARAIAVKKAAAARKKALENGGPVPYAGPIPSSCNDYSGSRAIGCALMLDKGFGLDQFPCLDKLWKHESGWNYRAENRSSGAYGIPQALPGKKMSSSGADWKTNPATQIKWGLGYIKGRYNTPCGAWSNFQNNGNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV11</accession>
    <name>U5VV11_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, AcrR-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12760"/>
    <dbReference type="EnsemblBacteria" id="AGZ40838">
        <property type="protein sequence ID" value="AGZ40838"/>
        <property type="gene ID" value="AFR_12760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2592"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_25_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011075">
        <property type="entry name" value="TetR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16859">
        <property type="entry name" value="TetR_C_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="22185" checksum="AD0828EC6D2CBDBC" modified="2014-01-22" version="1">MTDDFLHSPAVGRPRDPVLEQAILDATVEVVVGSGFSAAKVDEIARRARTGKAAIYRRWPTKTALVIAAAQRLQGDVAVPDEGSLREDLLACVRHYVSPDARTALILANVLAEAQQDPELREATITSIGQPPMEALRTVVERWIGRGEVDPSVPVDLVVSVAPSVAFHRVVVLHQALDDTTAVALVDGVLLPALGVPRISGSGRTGPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV88</accession>
    <name>U5VV88_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative multi-sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_12515</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12515"/>
    <dbReference type="EnsemblBacteria" id="AGZ40789">
        <property type="protein sequence ID" value="AGZ40789"/>
        <property type="gene ID" value="AFR_12515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2542"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_44_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="161"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="314"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="535" mass="58939" checksum="EFE2680C5F4CC9AB" modified="2014-01-22" version="1">MQALRATGLLDADSATSLDRLTRLATRLVKMPVALVSLVDVDRQVFVSAVGLEEPWASRGQTPLSHSFCQYVVTDQAPLVITDARADGRLCGNLAIRDLNVIAYAGFPLHSPDGQVLGSFCAIDGRPRQWTEDEVEILQDLAANAESEIALYTAHGELMLSSARMQTVLDTTQDAFVSMDHAGLVTAWNDAAHRLFGWSAAEAMGRPATELMIPERFHDAHRAGLERVRQGGRSRLSGQRVELIATDRDNREFPVEMTMQMAMERDRPVFHAFLHDITERRRLADQREQLLEQEQLQVRQLRELDKMKDDLVAVVSHELRNPIGVIRGYTELMAADPDLPEVLRQDLTVIDHTSARLTHLVDDLLDLAQLDAGKQFHLVPLDPGPLIQDAVRTHRITASARQVTLISEVEPLPVIPGEPRRLQQVLDNLLSNAVKYSLPGGTVTVTARHHDDRFVLTIADTGIGIPAEQYPHLFDRFFRASTATGLGIKGTGLGLAVTKAIVEAHDGTISAEPGTDGGTRFIVSLPARVQLTAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVH8</accession>
    <name>U5VVH8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane flavodoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12630"/>
    <dbReference type="EnsemblBacteria" id="AGZ40812">
        <property type="protein sequence ID" value="AGZ40812"/>
        <property type="gene ID" value="AFR_12630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2565"/>
    <dbReference type="eggNOG" id="COG4097">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_19_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="2">
        <location>
            <begin position="216"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="439" mass="48122" checksum="4CD09F406D881DB0" modified="2014-01-22" version="1">MPARHVGPATIAGLVLVQGLMWMVSRPPGEGLTTYLGQFFGAEAVLLLSVALVLISMLPWVEAWFDGIDRAAIWHRRLAITGLVLLAPHIALASNPTATQLGGILAVTGVAGMVGLALWAILPRWQSVVPRPLRPLVLAARDAPGVTAFRRIFGGYERWRALHRTTGLFVAAGFIHGVLDGTAFGGSPLLYWSYLAVGAIGLGFYLYRELLARFFRSLHDYQVATVTEVEPRLVEIALHPLGRGVDFVPGQFAMVYLEAKDGWHRHPFTIASAPHDKNLRFTIKALGDYTSRLHELVEPGMPAVIGGPHGRFDHRRGTDRQIWIAGGVGVAPFLSWLRSLDGPLPYRVDFFYSAVGTPAFADEIRTIADRHDDLHAHIIDTSLEGRLTAERVLVDAGGNPGGLSAFMCGPAAMLSDFETQLRRAGVASRHIHREYFDWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVK8</accession>
    <name>U5VVK8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_13735</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361092.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13735"/>
    <dbReference type="EnsemblBacteria" id="AGZ41033">
        <property type="protein sequence ID" value="AGZ41033"/>
        <property type="gene ID" value="AFR_13735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2789"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="148"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="23896" checksum="A972E45A8C1E2FA7" modified="2014-01-22" version="1">MTIRVLIVDDQSVYRTGVSAILAAQPDISVAGEAADGEQAVSQSRALRPDVILMDIRMPKMNGIDATRKLTGPDSTTRVVILTTFDFDDYVYEALEAGASGFLLKDADADELATAVRVVAQGDSLLAPRVTRRLIEEFVSNKPTGLRSTTVFNALTDREREVFQLIASGRSNAEIGQELFIAEQTAKSHVSRIMTKLSLRDRVHAVMLAYETGLVKPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVT1</accession>
    <name>U5VVT1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07705"/>
    <dbReference type="EnsemblBacteria" id="AGZ39830">
        <property type="protein sequence ID" value="AGZ39830"/>
        <property type="gene ID" value="AFR_07705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1571"/>
    <dbReference type="HOGENOM" id="CLU_1493133_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="180" mass="19065" checksum="8E1921096F39459D" modified="2014-01-22" version="1">MIEVIVSLALISMLMASLGTYFVSSLRTSRYQAQIQTATRLAQTGMEQARGFGGPALLVGRKQCGTCVNVSGFDTLGYLGATTRWDAKVTGAAPTVPIPETVSTDTFKVNDVTYSRYWFVGKCWQAAAGGACGTNSGLPVAMVRLVVGVTWLSPDCKFTICIRASSSLFSASAADPVFPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW23</accession>
    <name>U5VW23_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07835</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359387.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07835"/>
    <dbReference type="EnsemblBacteria" id="AGZ39856">
        <property type="protein sequence ID" value="AGZ39856"/>
        <property type="gene ID" value="AFR_07835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1600"/>
    <dbReference type="eggNOG" id="COG3296">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110679_0_0_11"/>
    <dbReference type="OrthoDB" id="2012653at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019109">
        <property type="entry name" value="DUF4870"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09685">
        <property type="entry name" value="DUF4870"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="24751" checksum="15813C60061BB853" modified="2014-01-22" version="1">MTEPPRPPGEGNPSDPTAPLNPYPGNDPAPGSAPPPPPAYGSPQPPTYGTPPPTYGSPPPSSGAGGYGPPPSSGAGGYGPPPSSGAGGYGPPPSSGAGGYGPPPSSGAGGYGPPPGGTPYGAPGAYGQQPGGYPPQGYAPTNDDKTWILVAHFGGAAGAFLGGGCSGWIAPLVALLAKGNQSPAVRAEAVKALNFQILWSIIAIVGWVLTCALVGFIIGPAAWAIGTIFGIVAGVKANNNEPYNYPMTVSMIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW66</accession>
    <name>U5VW66_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361133.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13940"/>
    <dbReference type="EnsemblBacteria" id="AGZ41074">
        <property type="protein sequence ID" value="AGZ41074"/>
        <property type="gene ID" value="AFR_13940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2828"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022176_3_0_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="391"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="530" mass="58365" checksum="7AA1ADF2C5E0F83E" modified="2014-01-22" version="1">MIHLSPVPAPVEAARVAALAAELDELENHESHDVQEILARATAAAEAADRSGDEVLGHRARLVQADMRRREGDLGSAARTFLATHRWAEQNDCRPLRARSHFHLALTYHYLGDHAASLEHALSAVELLDDDEPHGLRIIHLIRLANSLAEAGSTEAARDRYRQAERIAVGIGDLTRQLLVLNNLAYTEHEAGNVDIAWSAVERMRAVARAIGREFLIVERDTIANIQVSLGRYAEAEETLRDVYDSPPWFEAHDVAEASLTLAAAQRRLGALDRAQASLDHCRSLCDEHQLAGVRVRGLAEQAELYAAAGDHLAAFEEYKRFHAATEELRSTQLEARARSRQAMFETAEARRDAEHYREQARRDPLTGLYNRRFIDERLPQAMAEALAAGTPLTVALIDLDFFKRINDTLSHDVGDRVLVAVAVLLSARQDSLAPDGFAARMGGEEFLVVLPGVSAADAPERLETIRHDVASYRWAPTTGELPVTVSIGAVSVRAEGDQSQAALLAEADRRLYAAKDTGRNRVVARPVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWH9</accession>
    <name>U5VWH9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endo/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361422.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15405"/>
    <dbReference type="EnsemblBacteria" id="AGZ41363">
        <property type="protein sequence ID" value="AGZ41363"/>
        <property type="gene ID" value="AFR_15405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3127"/>
    <dbReference type="eggNOG" id="COG3568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010649_0_0_11"/>
    <dbReference type="OrthoDB" id="1387978at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="237" mass="26635" checksum="50C550E675A513C7" modified="2014-01-22" version="1">MRVLTYNLWGRRGDWPARRAVMAAELRRLQPDLAVLQETVVLDGYDQVVDLLGADYHVVHQQGRTADGTGASIASRWPVTDVRQHYLHVTSRVDTAEPWIGSVVLVDVEAPPPYGRILLTHFKPSWQFALAHERELQAVAPARLVEQAVAADAPDHVILAGDFDAAPESSMVRFWSGRQSLGETSVAYRDAWEDANGVEGGLTFVPENPLREQGDIRFEIGRRIDYVFVRADRWGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWJ5</accession>
    <name>U5VWJ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polypeptide N-acetylgalactosaminyltransferase 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361437.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15480"/>
    <dbReference type="EnsemblBacteria" id="AGZ41378">
        <property type="protein sequence ID" value="AGZ41378"/>
        <property type="gene ID" value="AFR_15480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3143"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044083_0_0_11"/>
    <dbReference type="OrthoDB" id="1247435at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665416" evidence="1">
        <location>
            <begin position="32"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="241"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="367" mass="38110" checksum="6E3DC7175D0AB7AD" modified="2014-01-22" version="1">MHGLLRRAGAAALGLALATGLLTVSPTAALAESNGGVRVMPLGDSITDGFNVPGGYRINLWQRMAADRLTVDFVGSGFNGPAALGDHDHEGHSGWKIRDLDANITGWLQRSAPRTVLLHIGTNDINGNDDVGNAPALLSALIDKIRVLAPNVELFVATITPISDAAAEQRARTFNATIPGIVAQKGSRTHLVDLHAALTTADLADGLHPNAGGYDKMAARWYSALQSVPGSLTAVTVPPVGSPALLSHGPSTRCLDISGGTTDGSQALIRDCHGGANQRFTRTAAGELKVLDRCLDVNGSGTANGTKVQLWTCNGTGAQRFTANPDGSLVNTNSLKCIDVSGNGTANGTLVQLYECNGTGAQRWTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWM1</accession>
    <name>U5VWM1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_14935</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361328.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14935"/>
    <dbReference type="EnsemblBacteria" id="AGZ41269">
        <property type="protein sequence ID" value="AGZ41269"/>
        <property type="gene ID" value="AFR_14935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3033"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="305"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="368"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="386"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="429"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ41269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="535" mass="54641" checksum="29784FBB5E5A810B" modified="2014-01-22" version="1">MSSRTDKNPGNGPVLGGRYQLISQIGVGGMAVVWQAYDTVLARTVAVKVLAGHSADDPQSRERIRAEAQAAAVLSHPNIAQVYDYGEAESGGALIPYVVMELVRGGTLQRRLSEGPVLPRYAMRLTAEIAAALAAAHAEGLAHRDIKPGNVMLAPTGAKVVDFGIAAAIRPPGSGTDDFEVFGTPAYLAPERLLHDAVEPASDVYALGVLLYRLLAGHSPWTSETTTQMLTAHIYLEPAPLEQMPQVPPYVTALCNRCLAKDPTERPSAREAAALLAHAAGLQVVTDEPPAQPAAIVDTEPSVLIRALGSPPPPPPPADAEPAAVPSPPAGDDPGSARRIRPVLAVVAVVLLMAVAVSVWLLRPTDETAAESAPRFGSTGAPAPSRGATDPPPGASPAPATTAPGDPAAGPTATTTTTAGQQPANAISPGDPAAPPPPPPSPGATPRTPAPEPTATTTTPPAEPVERTLSSSAGSVRATCPEAGTAQILSWTATKPYKVTQGDKDPGPSPAVSFKHGNKQVTMTVTCSGGVPSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWY6</accession>
    <name>U5VWY6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09310"/>
    <dbReference type="EnsemblBacteria" id="AGZ40151">
        <property type="protein sequence ID" value="AGZ40151"/>
        <property type="gene ID" value="AFR_09310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1897"/>
    <dbReference type="eggNOG" id="COG4587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084465_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010390">
        <property type="entry name" value="DUF990"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06182">
        <property type="entry name" value="ABC2_membrane_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="31404" checksum="D3AA71EB1A92C043" modified="2014-01-22" version="1">MDGLDARVPQISPRRPFRDSFATFGALAWSGFQRYATYRQATIAGSFTNIVFGFLRCYVLLAVAAGVAGGRPAGYGPEQLATFVWVGQGLLTVVSLWGWTELSDRIRSGEVTADLLRPVHPVIGYLAPDLGRAWHAMIFRFVPPLVVGLIFFDLYLPGRWFTVPLFAVSIVFAVIGSLAMRFLVNATAYWLHDARGPIMLWTLSAGVLAGLYFPLRFLPDWLAVTLWVATPFPGFLQTPLDVLIERDAAPLQAGLVVLQAVWAAVLLALCAVVQRRAERRLVAQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXE4</accession>
    <name>U5VXE4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360104.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10210"/>
    <dbReference type="EnsemblBacteria" id="AGZ40331">
        <property type="protein sequence ID" value="AGZ40331"/>
        <property type="gene ID" value="AFR_10210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2081"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069129_4_0_11"/>
    <dbReference type="OrthoDB" id="1515497at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="42"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="26511" checksum="D56031988B89F56B" modified="2014-01-22" version="1">MSMFNDPGLFGRQWAAQYDATSNPDPARAVDFLAGMAGHGPVLELAIGTGRVALPLAARGIAVEGVEGSPEMVEKLRAKPGGADLPVVIGDMADVGVRGPYTLAYLVFNTLFNLVDAQRQADCFRNVARVLAPGGAFVIEAFVPNLADFERDEAQPQVRHVTEDSASFRMHRYDRAAQTFVRQNVTFSAAGVRLEPFGMSYLWPEQIDRLAEQAGLHLEARYAGWERAPFDAASTDHVSVYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXF5</accession>
    <name>U5VXF5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="7">PRA-CH</shortName>
            <ecNumber evidence="7">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hisI</name>
        <name evidence="9" type="ORF">AFR_10285</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10285"/>
    <dbReference type="EnsemblBacteria" id="AGZ40346">
        <property type="protein sequence ID" value="AGZ40346"/>
        <property type="gene ID" value="AFR_10285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2095"/>
    <dbReference type="eggNOG" id="COG0139">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_5_1_11"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="SSF141734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PRA-CH" evidence="8">
        <location>
            <begin position="38"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ40346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13188" checksum="B92502845411A06A" modified="2014-01-22" version="1">MNGPVSALDPAVAARLRRSSDGLVAAIVQEAGTGDVLMLAWMDDEALHRTLTSGRATYWSRSRQEYWVKGATSGHHQYVKSVSLDCDGDALLVTVEQVGAACHTGTHTCFSDNLPVTDGEGARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXS1</accession>
    <name>U5VXS1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="3">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_16930</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361803.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16930"/>
    <dbReference type="EnsemblBacteria" id="AGZ41664">
        <property type="protein sequence ID" value="AGZ41664"/>
        <property type="gene ID" value="AFR_16930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3435"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_0_11"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="6">
        <location>
            <begin position="133"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="148"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="471" mass="48967" checksum="6F17AADA228C95F9" modified="2014-01-22" version="1">MSDRTFLLPDLGEGLTEAEVVRWLVTEGDTVAVDQPVVEVETAKSLVEVPTPYAGRIATRHAPEGATLEVGKPLLTVAALVPAAETYREEEQAGSGNVLIGYGTTGASATSRRRRPRAALQPAAPAAGRAPLVISPLVRRLAREGGLDLRSVTPSGPGGIITRADVDRALTRPSASPQAAESGSTRAWAPAQADDFAAAASASPAQAADLRSTASVASAQAAGLGSTAFAAPAQAAGSVAAAPAVAGERRIALSGFRKAVSAALTRSRAEIPEATVWVDVDATPLWELRASTPGGPGLLAYIARFVVAGLRQYPMLNARVDTERQEIVEFERVNLGIAVQGERGLVVPAVMDAGNRTTAQLDEEIRRVTASAREGRATAQELSAGTFTLNNYGSFNVDGSAAIINHPQVAILGFGRIIDRPWVVGGEVCVRKITQMSFVFDHRVCDGGTAAGFMRSVADAIEDPASAIARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY26</accession>
    <name>U5VY26_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11580"/>
    <dbReference type="EnsemblBacteria" id="AGZ40605">
        <property type="protein sequence ID" value="AGZ40605"/>
        <property type="gene ID" value="AFR_11580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2360"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044042_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="64"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="486" mass="54095" checksum="71AEE85198A9C560" modified="2014-01-22" version="1">MTEELIREVLRYTDVAILAYFVALNSSYLVLIVMAGLEFGKHLRRVPFAGADDMFRSPLTLPVSVIVPAYNEGAGIVAAVTAMTSLRYPRYEVVVVDDGSSDDTFELLREQFDLVEVPRVVPAEIPYQSQVLSVHVARANPETLTVVRKTNGGKADALNVGINLARHPLICMVDADSVLDPDALLSVAKPFGDDPLRVVACGGVVRIANGCKVVGGRVVDVRMPKQWLVRIQVVEYLRAFLMGRTGWSRLGGLVVISGAFGVFRRDLVVQVGGMDPATIGEDAELVVRLHRHLRERKQDYRVIFVAEPVSWSEAPSSRKVLRRQRRRWHRGIAEILGKHRRMILNPRYGRIGLVALPYYVVFELLAPFIELGALVLLPLGRWAGAVDTAFLWRFILVAYGYGLLVSLISLFIEEVSFHRYPRWGDLVRGVAAAVIENFGYRQMLAVWQVAGAFGALHGRKAEWGTMHREGFDTAESREPVSVGVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY70</accession>
    <name>U5VY70_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_17805</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362151.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17805"/>
    <dbReference type="EnsemblBacteria" id="AGZ41839">
        <property type="protein sequence ID" value="AGZ41839"/>
        <property type="gene ID" value="AFR_17805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3611"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006937_8_1_11"/>
    <dbReference type="OrthoDB" id="630753at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="536" mass="59365" checksum="811ABF4B1260FC3A" modified="2014-01-22" version="1">MSASAPNRSVDVVVVGAGFSGLYLLHRLRELNLSAVVFEAGTDVGGTWYWNRYPGARVDVESLAYSYSFSEELEQEYPWEERYPTQPEILRYAQHVADRFDLRRDIVFRTRVAAAHWDDEAGTWQVRTEHEDAVTARFLVLATGCLSASKLPEIPGLDTFKGATFHTAHWPHEGVDFTGQRVGLIGTGSSGVQSIPVIAEQAADLTVFQRTPAYSLPARNRRLRPDEIADMRAGYRAYRKAQRESGFGVPTPVPTKSALEVSDEERDATFERAWESGSLVNLLTAFTDTAVDQAANDTARDFIHRKIRQIVTDPQVAGDLCPGYPVGTKRPCLDTGYYETYNRPDVHLVNLQREPLVEVTERGIRTAEREYAFDAIVYATGFDAMTGALTNIDIRGRGGVSLKETWSAGPRTYLGLGSVGFPNMFMITGPGSPSVLSNMVVSIEQHVEWVTGVIEHLEKQGLRAIEPTQQAQDSWVDHVNEIAGYTLYPKANSWYMGANVPGKTRVFMPYAGGVGAYRKRCDEVAAQGYEGFALTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYG7</accession>
    <name>U5VYG7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362307.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18280"/>
    <dbReference type="EnsemblBacteria" id="AGZ41934">
        <property type="protein sequence ID" value="AGZ41934"/>
        <property type="gene ID" value="AFR_18280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3710"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_4_11"/>
    <dbReference type="OrthoDB" id="664909at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="36170" checksum="9B43B00356CDD922" modified="2014-01-22" version="1">MATLADVARRAGVSTATVSRIINNSPKPVAEALRERVLAAVAELQFVPNANAQQLARADRTAVGVIVHDVSDPYFAEITRGLQRVATDHGRLVIICNSYRDPERELAYVDLLHAHQTAAIVLAGSGYHDAPITARLDAKLRIYESTGGRVAVIGRHEHSGDAIVPENEPGGYLAGDALYRLGHTTIGVIAGPRHLTTTTDRLAGLRRAAKEHGHRLTARQVVYADFDRNGGRAAAAELLDRFPALTAVAALNDSMAVGALACLRERGIDVPGQVSLIGFDDMPISVDVTPPLSTVRLPLEDIGVRAMSLALREGERTTPLVEHAAAELVLRGSTAPPSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYM5</accession>
    <name>U5VYM5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">DNA ligase</fullName>
            <ecNumber evidence="10 11">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ligA</name>
        <name evidence="13" type="ORF">AFR_18045</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="10 11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18045"/>
    <dbReference type="EnsemblBacteria" id="AGZ41887">
        <property type="protein sequence ID" value="AGZ41887"/>
        <property type="gene ID" value="AFR_18045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3662"/>
    <dbReference type="eggNOG" id="COG0272">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007764_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00114">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018239">
        <property type="entry name" value="DNA_ligase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033136">
        <property type="entry name" value="DNA_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNAligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="DNA_ligase_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="SSF52113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01055">
        <property type="entry name" value="DNA_LIGASE_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01056">
        <property type="entry name" value="DNA_LIGASE_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 10 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10 11" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="BRCT" evidence="12">
        <location>
            <begin position="622"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="10">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="55"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ41887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="706" mass="73442" checksum="C800506EE6ADA306" modified="2014-01-22" version="1">MTSASPELTGHEVAPVVDAAKAASFGTAEDYRAAIARIRSAAESYYAGTDLAMDDATYDALLARVAATEQAEPGWAEAGSPTTTVGAGVAGGDVEHSTPMLSLDNVFDADELRTWAARLDRVLGRPAQGYTVEPKIDGMAIAARYTDGRLRQIATRGDGRAGEDVTTQARLVAGLPAELTRPVTVEVRGEVFMTDADFARANELRVAHGGQAFANPRSAAAGTLRAQDRAYDAPLSFLAYAVHDLPGGEGLTHSAAMAAIAGLGVATTAGSPAGMPQCATVDDLLAAITAMGEARGTLGFDIDGAVVKADDPTDRDRAGSSSRAPRWAIAHKFPADTRTSKLAAIEVQVGRTGVITPVAVLEPVQVGGVIVTSATLHNFDDLVRRDVRVGDTVFVRRAGEVIPEITGAKLDERPADARPFTPPEVCPRCGGEIDRTQKRWRCVQGRACGAGESLSYFAARDSMDIEGLGDKVIAGLVAAGLVTDPADLYDLDVEALSRLDRLGRTSATKLVANIEASKAQPLSRVLTGLGVRMTGRSMSRRLARHFGSMEALTSATVDELQEVEAVGPERAATIAAELTDLAPVIAKLTARGITMTEPGAPTSSARPATLPLPLQKEDGKPMTVVVTGSVPGLTRNEGNEAVETMGGKSSGSVSARTDLVVVGEGAGSKAAKAETLGVPIMASQRFADLLTEHNAGSSPTLADYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYN8</accession>
    <name>U5VYN8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360899.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12770"/>
    <dbReference type="EnsemblBacteria" id="AGZ40840">
        <property type="protein sequence ID" value="AGZ40840"/>
        <property type="gene ID" value="AFR_12770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2594"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_1_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="31681" checksum="D0DD2D57B1010B00" modified="2014-01-22" version="1">MDQRDLADFLRRRRELLQPADVGMPAGARRRTPGLRRHEVAQLAGMSPDYYIRLEQARSPQPSPQMLTALARALRLNRDERDHLFHLAGHPAPPAFGADDHVSPGLLRLLDAFTTLPAQIVSDLGAVLVQNQMAVALLGEQTAGTGLSRSFIYRWFTDPGIRALYPPEDHEHQSQVQVADLRAAVALRPRDALADKLVRALRAASDEFSALWDRHEVAVRRSDRKRIVHPAVGTIDVDCEVLTTARQDQRLLVFTARPGTDAVGQLELLRVIGSQNLEAVTGASAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYP4</accession>
    <name>U5VYP4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362280.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18145"/>
    <dbReference type="EnsemblBacteria" id="AGZ41907">
        <property type="protein sequence ID" value="AGZ41907"/>
        <property type="gene ID" value="AFR_18145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3683"/>
    <dbReference type="eggNOG" id="COG2021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_0_1_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="32"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="30638" checksum="ADAC4E5F1CF750FE" modified="2014-01-22" version="1">MTSEDIRLHHETLGDPGDPALLLIMGLGAQLIDWPQEFCEQLAARGFHVIRFDNRDAGLSTRRPEWGVPDLPAILAGDRTTVPYLLADLAADAAGLLGTLGIERAHVAGASLGGMVAQQLTIDHPDRVLSLCSIMSMTGDLTVGRPTPEAAAVLGRPPAPDREAAIANSVASSRIIGSPGFPAAEEELLRRAAAKYDRAYHPLGTLRQYAAVLASPDRTAALHGVKVPTVVIHGEDDPLIGVSGGRATAAAVPGAELIVIPGMGHDLPREVWPRIVEAIAANAGRAHGDHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYQ8</accession>
    <name>U5VYQ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alkyl hydroperoxide reductase/ thiol specific antioxidant/ Mal allergen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_12870</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360919.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12870"/>
    <dbReference type="EnsemblBacteria" id="AGZ40860">
        <property type="protein sequence ID" value="AGZ40860"/>
        <property type="gene ID" value="AFR_12870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2614"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033708_0_0_11"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041017">
        <property type="entry name" value="Thioredoxin_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17991">
        <property type="entry name" value="Thioredoxin_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="262"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="565" mass="59143" checksum="D6C02D12205C0309" modified="2014-01-22" version="1">MITLALIGFVGGLITGISPCVLPVLPVVFLSSGTAAGRESTGRRPLLVVLGLTLSFSVFTVLGSLVLRALPVPQDIIRWAGIVILALLGIGMIVPRFEAWLERPFARLAPRAPGQDRGGFLLGLALGAVYVPCAGPVLAAIAVAGATGRFGASTLVLIATFALGTAIPLLIFALAGWRITDRLRSFRTRQRGIRIAAGGVVVALAVALTFNVTDAVQRAIPDYTSALNDRIDAAGAVAGALTGGGASTALAACQETAGQVAPALSDCGPAPQLTGLDGWLNTPGNAPVTPAQLKGKVVLVDFWAYSCINCQRAIPHVNAWARAYAGSGLEVIGVHTPEYAFEHDAGNVAAGAQRLDIGYPIALDNNFKTWDAFHNQSWPADYLIDATGRIRYAGVGEGQYPQTEELIRRLLSTAHPDVILPAATDVPDRTPTASWQSPETYLGAARAQYYSGKTPLHPGKATYTLPGDLADTSYAYGLSGTWTVTDESITAEKDSAITLNYTARDIYLNLSGTGTVKVTVQGKTQTYEVSGAPNIYTVLHRPRLGGGTLTASLSPGLSAYSFTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYS3</accession>
    <name>U5VYS3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_19130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19130"/>
    <dbReference type="EnsemblBacteria" id="AGZ42098">
        <property type="protein sequence ID" value="AGZ42098"/>
        <property type="gene ID" value="AFR_19130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3885"/>
    <dbReference type="HOGENOM" id="CLU_2566176_0_0_11"/>
    <dbReference type="OrthoDB" id="2091290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="75" mass="8541" checksum="FF33D14089C55805" modified="2014-01-22" version="1">MRKIACPVCGQDWLRWYRVKDDGLTFLLCPECDSVWLPGQDPSPTARQDLSELLGYPQVHGTEWDLIEPCAAPHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYS4</accession>
    <name>U5VYS4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DeoR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12650</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360875.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12650"/>
    <dbReference type="EnsemblBacteria" id="AGZ40816">
        <property type="protein sequence ID" value="AGZ40816"/>
        <property type="gene ID" value="AFR_12650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2569"/>
    <dbReference type="eggNOG" id="COG2378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041141_1_0_11"/>
    <dbReference type="OrthoDB" id="1654295at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028349">
        <property type="entry name" value="PafC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018356">
        <property type="entry name" value="Tscrpt_reg_HTH_DeoR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016838">
        <property type="entry name" value="PafC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00894">
        <property type="entry name" value="HTH_DEOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="320" mass="35213" checksum="B8A61453FB2523C0" modified="2014-01-22" version="1">MPKTSARLLALLSLLQARRDWPGTLLADRLDISLRTVRRDVDRLRELGYPIAAIKGPDGGYRLSAGTELPPLLFDDEQAVALTIALQVAATTSGDTLAEAAVRALNTIRQVMPSRLRHRVEALRITAVERPTTRPAAPVDGDVLLAISAAVHARETLRFDYGPESTDTPPRRVEPHHVITWDGRWYLIAFDLDRNDWRTFRVDRIALRTPNGPRFTPREIPGGDVAAYAIGKFRGSDDAGGWPCQGTVILDLPAATVALYTRDGIVEEAGPGRCRLTLGSWSWPSLAATIARYDADIDVVGPTELTDAFAHLARRFTRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ57</accession>
    <name>U5VZ57_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13400"/>
    <dbReference type="EnsemblBacteria" id="AGZ40966">
        <property type="protein sequence ID" value="AGZ40966"/>
        <property type="gene ID" value="AFR_13400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2721"/>
    <dbReference type="HOGENOM" id="CLU_890339_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="32470" checksum="C16DB8B9DD6D8EAA" modified="2014-01-22" version="1">MGLVPAAMIGGAAVVTLGWQNRLPERLPNSGGVGDRPVLTTIGVDTVVAVLGEAAIAVWLLGLVAFALSRGLSGVGRHWVRTGVQVGVCGFVAAAASALLMLLAPALDAPSATDVRLSWGYFVACLVVPMLVAAVLGLVAYALAGKPPTDRPAGATPDERLLWWESRALRPVLWLSGALALSGVVLVAALVPLIGVAGWAGAAPAATALVLLPFARYRLVIDQDAVRCAVGPYRRRVPMSRILAAEEGYLPVGTWLTRSALHGAAADLPLLPGPALALRLTDGTRRLVTCRDVRTAVETVNTLRLRRTTAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZY9</accession>
    <name>U5VZY9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361349.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41290">
        <property type="protein sequence ID" value="AGZ41290"/>
        <property type="gene ID" value="AFR_15040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3054"/>
    <dbReference type="HOGENOM" id="CLU_1096797_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="27203" checksum="4F7DA7BB3F0580D8" modified="2014-01-22" version="1">MGADLVEMQRGFAANAADTERAVTEAVAGRRSRGGTMVALSSPGARSVVLAQVRRDGFDEVAVVGTPDVAVCTALGFTLHGFGDWVKTSPHDADVWPGDAAVAAAALATDIDLTRVGIAWCDGLHDGHDVVRARRVLLGYFGAGRQRFTLPPKIDQERLLSYDRDGRLARAVIAISRPSRLELTSLRHLEECGIRTAADFVGAQVDYLSVRRTAYVIRRDGTPLQIRGIGADRALGLSAWRQWIDAGRAPVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W039</accession>
    <name>U5W039_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH-type transcriptional repressor purR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361404.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15315"/>
    <dbReference type="EnsemblBacteria" id="AGZ41345">
        <property type="protein sequence ID" value="AGZ41345"/>
        <property type="gene ID" value="AFR_15315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3110"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_3" evidence="1">
        <location>
            <begin position="119"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="275" mass="28896" checksum="C76991D9D9597599" modified="2014-01-22" version="1">MTEAQRRVGLVLARASRVLGEEPYYHEFLEGLERVLTPAGVSVLVKVVTDRQAESDTYERWAARRHVDGVVLVDLAPGDDRVALVNSLGLPAMVLGAPSTASGLPTVWTDDAGFAHEAVRFLAGRGHEVIGHISGPVSFAHTQLRRSGAAAEAEALGLQLLHAEGDYSYESGRAATVELIGAGPTAIFCDNDLMALAALDTLRERGKSVPDDVSVVAWDDSPLCQLATPALSAMSHDVGRIGELAAGAMLDAMAGAAPVVYEAPAAHIVTRASTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0F0</accession>
    <name>U5W0F0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YjdG-like N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21495"/>
    <dbReference type="EnsemblBacteria" id="AGZ42569">
        <property type="protein sequence ID" value="AGZ42569"/>
        <property type="gene ID" value="AFR_21495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4360"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_28_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="164" mass="17829" checksum="96027D70211C5BA2" modified="2014-01-22" version="1">MIRFVELPPAALQALIESDLPAAGAAAGQELSEYILGEKWLWEMRFEQIARDPASAEWIARAVVAEPEQVVIGHAGFHGPPDADGMVEVAYSVAPAYRRRGFAKEMLAALIARAETDPRVRVVRASISPENAASLATIAGFGFVQVGEQWDEEDGLELLFERPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2C1</accession>
    <name>U5W2C1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Erfk/ybis/ycfs/ynhg family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_24480</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363752.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24480"/>
    <dbReference type="EnsemblBacteria" id="AGZ43162">
        <property type="protein sequence ID" value="AGZ43162"/>
        <property type="gene ID" value="AFR_24480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4958"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039404_0_0_11"/>
    <dbReference type="OrthoDB" id="975147at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041280">
        <property type="entry name" value="Big_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17964">
        <property type="entry name" value="Big_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665617" evidence="5">
        <location>
            <begin position="30"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Big_10" evidence="7">
        <location>
            <begin position="59"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="6">
        <location>
            <begin position="167"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32681" checksum="522F6CBBFFF25276" modified="2014-01-22" version="1">MKAFLATGTALAVVSLGAACSGHGPAASAADLPSSPTPSTTTPAASAGPADLPQPPSSAKFVRVTSFLGDGRTVGVAMPLILRFGRAVPVARRAAVEERLTVRSRPAQPGVWSWTSPTEVHFRPRHFWLARTAVTYRADLAGLPLGNGRYVRNNLGVGISIGRAFVMTVDNRTKTMTVERDGKVVKRIPVSLGKRSTPSSSGTMMVMDKQRHAVFDTFDELGADGYRTKIEYAQRLTWGGEFIHAAPWSEDAQGRTNVSHGCVNVSQRMGRWLFARTLPGDPVTIRGTSRKLRPGNGWTDWND</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2C6</accession>
    <name>U5W2C6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glucosylceramidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_24505</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363757.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24505"/>
    <dbReference type="EnsemblBacteria" id="AGZ43167">
        <property type="protein sequence ID" value="AGZ43167"/>
        <property type="gene ID" value="AFR_24505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4963"/>
    <dbReference type="eggNOG" id="COG5520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014379_3_0_11"/>
    <dbReference type="OrthoDB" id="447059at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004348">
        <property type="term" value="F:glucosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033452">
        <property type="entry name" value="GH30_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001139">
        <property type="entry name" value="Glyco_hydro_30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033453">
        <property type="entry name" value="Glyco_hydro_30_TIM-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11069">
        <property type="entry name" value="PTHR11069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02055">
        <property type="entry name" value="Glyco_hydro_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17189">
        <property type="entry name" value="Glyco_hydro_30C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00843">
        <property type="entry name" value="GLHYDRLASE30"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665472" evidence="6">
        <location>
            <begin position="23"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="7">
        <location>
            <begin position="496"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="517"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="635" mass="68508" checksum="8E178DD01B74AC63" modified="2014-01-22" version="1">MRRRAFGAAVSAALLILTTAPAQAHGPRDPHARVWVTTVDRAEQLHERAPVAFRHGGSPETTITVDPGTRYQSMDGFGASLTDSAASVLHRLDPAARDRAMRSLFDPREGIGVSFLRQPVGSSDFTATAEHYSYDDVPAGQTDFALKHFSIAHDQAQILPLLRRARQLNPQLKVMGTPWSPPAWMKTTDSLIGGHLKDDPKIYDAYARYLVKYVQAYAKAGVPIDYLTIQNEPQNRHDGGYPGAAVPVPQAAKVITALGPLLRRASPRTKILGYDHNWTTHPGDVGTTPPGEDPETDYPYRLLDSPAGKWLAGTAYHCYSGDPSAQTALHEAYPEKGIWFTECSGSHGENDTPEQVFRGTLTWHARTLAIGTTRNWAKSVVNWNIALDSTGGPHLGGCGTCTGLLTLQADGSVTRDAEYYTIGHLSKFVKPGAVRVASTSFGTTGWNGQIMDVAFRNPDGSTALVVHNENDDPRTFAVAVGGRSFEYTLPGGALATFTWPRGLTSDLDLLPIKGATATASPAGETPGAAVDGDGSTRWSSGTAQEPGQYLQVDLGRTQWLRRVAIDSGGNTGDYARGWELTASTDGSHWRTLARGTGTGQLTNVDVPLTRARHLRVTSTASSGSWWSIADLRLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2D6</accession>
    <name>U5W2D6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363767.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24555"/>
    <dbReference type="EnsemblBacteria" id="AGZ43177">
        <property type="protein sequence ID" value="AGZ43177"/>
        <property type="gene ID" value="AFR_24555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4974"/>
    <dbReference type="eggNOG" id="ENOG503410I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_784435_0_0_11"/>
    <dbReference type="OrthoDB" id="1136301at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666017" evidence="1">
        <location>
            <begin position="31"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="358" mass="37680" checksum="9CC28D0EA438C944" modified="2014-01-22" version="1">MRAARGVLALAIALAAGGALLVFAPDDKKAAPPAARVALAEAWPGAQRAEIAGNLRDGPIYHPGLFLDARTSVGTAPSPDGTAVRLLVRNADDTIRELRRRPLNKNPEFGNLTTGGDELVWTESADGVPVEIWTARTTGGPARKVTADTGNALFFGSQWDLALAGGEVHWAAASPQGDKFTEIRSVALAGGTVRKREEPGTWALSAWPWLTEGGGDQAGTTRLRNLDANRETRVEQSGAELATCSPVWCRVLVMNGDGLARIDVMHPDGSARQRIAGPAASAAITDVGVLDRFEILAEAGPDSDLTGTAGLLIYDITAGRTIDVSPAADGAFSRNGVLWWATGDQDALVWHTLDLRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2R6</accession>
    <name>U5W2R6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH-type transcriptional regulator betI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362647.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20015"/>
    <dbReference type="EnsemblBacteria" id="AGZ42275">
        <property type="protein sequence ID" value="AGZ42275"/>
        <property type="gene ID" value="AFR_20015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4061"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096009_0_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="19874" checksum="B5BA6BE4FB435419" modified="2014-01-22" version="1">MTRTRLTAEQRHEQLLGAALTAFTTGGYAGTTTDQVARLAGVSQPYVIRLFGTKQQLFIATVEHASSRIEERFRAAAAQQADLSALGSAYDELLAERELITVLLHGFAAAADPAIGPVVRDCFGRLYGVVRELTGAGAEETRDFFAYGMLLTVLGAMNAVGPGAVATDGPLAALVGSLGDDKEPAAKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Z0</accession>
    <name>U5W2Z0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rieske (2Fe-2S) iron-sulfur domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364259.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26185"/>
    <dbReference type="EnsemblBacteria" id="AGZ43499">
        <property type="protein sequence ID" value="AGZ43499"/>
        <property type="gene ID" value="AFR_26185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5307"/>
    <dbReference type="eggNOG" id="COG4638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1122721_0_0_11"/>
    <dbReference type="OrthoDB" id="691776at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044043">
        <property type="entry name" value="VanA_C_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19112">
        <property type="entry name" value="VanA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VanA_C" evidence="2">
        <location>
            <begin position="142"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="27414" checksum="386B0DABED278DF9" modified="2014-01-22" version="1">MQWLPVAREVSPGLLTNEDPRLRGAWHPVALTGELPCDVTLLGSRYSLVSREGVLHVQPEPWAVTERWGLVWIAPEEPRTELFLDADTADRTSVGAWLPPVRTPASADVELSEFMHARTFGAAEENQIPRYAVSTGPDGFKSADDRGATYVYRAPFQLLLRLEEPDAVRTILFFAQPETLTSTRVYTKLLLHGVADPGPEVVAREVAVLAEDLEVHRAMRQLLSPHDHADLLGVTLRRILADFVNGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W331</accession>
    <name>U5W331_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WYL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364329.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26360"/>
    <dbReference type="EnsemblBacteria" id="AGZ43534">
        <property type="protein sequence ID" value="AGZ43534"/>
        <property type="gene ID" value="AFR_26360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5343"/>
    <dbReference type="eggNOG" id="COG2378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041141_3_1_11"/>
    <dbReference type="OrthoDB" id="1654295at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WYL" evidence="1">
        <location>
            <begin position="152"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="36355" checksum="CCA3E2F7CAF3CFAC" modified="2014-01-22" version="1">MSRTRTERLVNLVICLLSTRRFLTAAQIAATVPGYEHDPEDPRDHEAFQRKFERDKAELRELGVPLETGLASAFDTESGYRIAHREYALPDIPLEPDEAAAVGIAARLWQHAGLAAAASSGLAKLRAAGVEVDPQATLGVEPVVTVDPAFGPLTGAARSRRAVTFSYRVPEVDEPSTRRLQPWGVVCWQGRWYVVGHDRDRDATRCFRLSRIVGSVRAVGSEGAFTPPENVDLISHVARWSGPVERNGRATVFVRHGRAAGLRRMAVETVPGPDGDRLTVPYSDVEWLASRLVGYGADVRADGPPELREAVIQQLKEVVVRHERVLPAVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3J0</accession>
    <name>U5W3J0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364404.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26550"/>
    <dbReference type="EnsemblBacteria" id="AGZ43572">
        <property type="protein sequence ID" value="AGZ43572"/>
        <property type="gene ID" value="AFR_26550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5382"/>
    <dbReference type="eggNOG" id="COG0645">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1473743_0_0_11"/>
    <dbReference type="OrthoDB" id="1574819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="20250" checksum="B1FA9E338829AD45" modified="2014-01-22" version="1">MRRLVHLNGPPGIGKSTLAALYADRHPGALNLDVDAVHRLVGGWRDEELDTWPITWALVRAMAAAHLDGGHEVLLPQYAAGSDDITAVEKLAHEHGATFHEIVLLDERDAAIERFGRRARDSDDPWIRHHHRLLEQSGGSAALGAMYDRLVKALNPGAVQITCRAGAVEETYELLVRVLRGLSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3V5</accession>
    <name>U5W3V5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560235.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28185"/>
    <dbReference type="EnsemblBacteria" id="AGZ43898">
        <property type="protein sequence ID" value="AGZ43898"/>
        <property type="gene ID" value="AFR_28185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5713"/>
    <dbReference type="HOGENOM" id="CLU_1691711_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666052" evidence="2">
        <location>
            <begin position="29"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="15899" checksum="49EA2E933DD1CE93" modified="2014-01-22" version="1">MKRLGPALLITTALVSGCTQQQQQPAPAPSISPSPSTVDAVAAADGTDAKACEDGRCQILVAQQSDFALDGKFNCDGILITFTAPKEVEFDVSVQDGDDLHATVKGTGKLALAYGLTLTVEQTGPAGAVLRVAPAKNDPDNHTGTGTEGFSLWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3W2</accession>
    <name>U5W3W2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_21630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362968.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21630"/>
    <dbReference type="EnsemblBacteria" id="AGZ42596">
        <property type="protein sequence ID" value="AGZ42596"/>
        <property type="gene ID" value="AFR_21630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4385"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_11"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665665" evidence="2">
        <location>
            <begin position="26"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="HTH lysR-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="31979" checksum="DF2B1010BF4D4DAC" modified="2014-01-22" version="1">MLDVRRLRLLCDLSRLGTIAAVAQAHTYTASAVSQQLSALEREAGVALLERTGRRVTLTPAGNVLVEHAVTVLAALERTTAALAAVRSGLSGPLRIGAFPTAVRTLLPEALVELGRHHPGLELMVTELDPVDVPAALRERRLDVGLVHDYDVVPDSPDPALDATPLLDETVFLAVPARSAHTTLDDVRDAAWIVAGPGTLCHTATLRICGAAGFAPRARHQADDFATVLALVAANQGVSVVPALAAAQPPDGVRLIALPTRRRNRIAYRRGAAAHPAIAAFVTAIRARAGVSGLPSAPHTTRIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Z3</accession>
    <name>U5W3Z3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phospholipid/glycerol acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363071.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22150"/>
    <dbReference type="EnsemblBacteria" id="AGZ42700">
        <property type="protein sequence ID" value="AGZ42700"/>
        <property type="gene ID" value="AFR_22150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4490"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_4_0_11"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="1">
        <location>
            <begin position="34"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="23287" checksum="D312296C0679A3F3" modified="2014-01-22" version="1">MLAEGARSVLVPVSRLYFRPTIEGREHIPRSGPVILAANHLSALDSFLIPLVSPRPVAFLAKEEYFTQTGAKGWLIRTSLLGVGAVGVPRGAHRAAQAALETALAVLQEGRMFGIHPEGSRSRDGRLYRGRTGVAWLALASGAPVVPVAVLGTEHAQPVGTRIARPRHVTVRFGAPLRFAPPAGSAGKARRDATDEIMDAIAALSGQVRAPGYNELSALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W461</accession>
    <name>U5W461_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metallophos domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28710"/>
    <dbReference type="EnsemblBacteria" id="AGZ44003">
        <property type="protein sequence ID" value="AGZ44003"/>
        <property type="gene ID" value="AFR_28710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5820"/>
    <dbReference type="eggNOG" id="COG1408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025443_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="130"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="351" mass="37652" checksum="D1DE76E36D86D76B" modified="2014-01-22" version="1">MRRTGSVMFVVGPLLLVLALPHDLPGVPFRAEGVVDWLGFMWLALFLYLLLALVVGEAARPLLHRLPAAVDAPREPVLSRRVFVSRVVGGGAAAVALGTVGFGAYGVLRGPRVKRITVPLAKLPRSAHGFRIAVVSDLHLGPLLGRGFAQHVVDTINSTQPDLIAVVGDLVDGSVQDLGPAVAPLAQLRARHGSFFVTGNHEYLYDARRWIDVVRDLGVHPLENALVELPGFDLAGVNDVEGKRQEQGPDFARALGDRDRSRTAVLLAHQPRMIDEAVEHGVDLQLSGHTHGGQLWPGPYVAELVMPTVAGLHRYGDTQLYVTRGAGAWGPPVRVGAPSDITVIELASRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W498</accession>
    <name>U5W498_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glucose/sorbosone dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_28490</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560296.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28490"/>
    <dbReference type="EnsemblBacteria" id="AGZ43959">
        <property type="protein sequence ID" value="AGZ43959"/>
        <property type="gene ID" value="AFR_28490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5776"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012253_3_0_11"/>
    <dbReference type="OrthoDB" id="567019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666349" evidence="3">
        <location>
            <begin position="32"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="4">
        <location>
            <begin position="23"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="264"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="690" mass="71762" checksum="E0D170333F2DAB15" modified="2014-01-22" version="1">MLTRVRALVAGAAVVVVAAGAVVAGTVQASAAAGSTVVGVASGRCLDVVGNRAEPQTGINIYDCNGQANQAWQLTAAGELRVFDGALCLDVVGQDTSAPAAVQTYACTGGANQRWRVGTDGTVVGVQSGLCLDVTGGATANSTPVGLWTCNGQSHQKWTTALGSADTTAPTVPGNARVSDLVCDSVTFAWDASTDAVGVAFYDVYHDGQLMKSVSGTTLSTSLTVVGGVTWGLYVNARDAAGNVSQASRTVPITPPQCQEDTQPPSAPARLTGAVSGTTVTLNWDASSDNIGVRAYDIYRGGAKIGAVTGTASAPPATGFIDSGLSGTVRYEYFVVARDAQANVSARSNTVAVTTGSSCTNPVCEVRQVGADTDIPWGLVTLPDGSILYNRRDAHDIVRLDPATGARSTVGTVPDVQSTDGEGGLTGLAISASFASDRWLYIMHTSATDNRIVRIKMVDDRLDLASKQVLVSGILRNKFHDGGRLRFGPDGKLYASTGDAQNGDNAQNRASLNGKVLRLNPDGSVPSDNPFGNYVWSYGHRNPQGLAFDSQGRLWEQEFGNAIMDETNLITKGGNYGWPACEGTSGTCNTAGFLAPKRTYPTSDGSCAGIAVVRDVLYIACERGERMYRAVISGTELTGVQAYFTGIYGRLRTVEPAPDGGLWLTTSNGGDKDSIPNNTDAKILHVTLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4H3</accession>
    <name>U5W4H3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363321.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22780"/>
    <dbReference type="EnsemblBacteria" id="AGZ42826">
        <property type="protein sequence ID" value="AGZ42826"/>
        <property type="gene ID" value="AFR_22780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4617"/>
    <dbReference type="eggNOG" id="COG4106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067079_1_0_11"/>
    <dbReference type="OrthoDB" id="1414639at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006764">
        <property type="entry name" value="SAM_dep_MeTrfase_SAV2177_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04672">
        <property type="entry name" value="Methyltransf_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017393">
        <property type="entry name" value="MTase_SAV2177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28552" checksum="B31819155E79911C" modified="2014-01-22" version="1">MAEPLDTGRPHPARVYDVLLGGKDNFAADRAAAAEGLKVNPNAATAPLQNRAFLQRAVRFLAGEAGVRQFLDIGTGLPTSPNVHEVAQAIDPTARIVYADNDPIVLTHARALLTSSPEGRTAYVDASLQDVDGILKAPELRETLDLDEPVAVLLLAILHFLDDDADPWSIVDRLMAGLPSGSWLVLSHITDDHDPESWARFAAIMRRQGITTRLRGHDDVARFFTGLELVEPGVVPILRWRPDHEETLTDAQVALYGGVASKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4I2</accession>
    <name>U5W4I2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MarR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363411.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23150"/>
    <dbReference type="EnsemblBacteria" id="AGZ42900">
        <property type="protein sequence ID" value="AGZ42900"/>
        <property type="gene ID" value="AFR_23150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4693"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_4_2_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="17600" checksum="1B9E3F1A5AFEF707" modified="2014-01-22" version="1">MSESAAPPGDPGIQDLGWALTTVLRNYLHAVGAAVSDVPGGPRGYQVLNAAAGGHCRNQAAIAELLAVDRTVMTYLIDDLERSGLVERRPDPADRRSRQVLLTAKGKQTHTRLRERITDAERHVLGGLSGAEVEALRGLLGRAALHADNGNHPIDACTAAAELTATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5M5</accession>
    <name>U5W5M5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Urease accessory protein UreF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ureF</name>
        <name evidence="4" type="ORF">AFR_30920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for maturation of urease via the functional incorporation of the urease nickel metallocenter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UreF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30920"/>
    <dbReference type="EnsemblBacteria" id="AGZ44444">
        <property type="protein sequence ID" value="AGZ44444"/>
        <property type="gene ID" value="AFR_30920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6259"/>
    <dbReference type="eggNOG" id="COG0830">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049215_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01385">
        <property type="entry name" value="UreF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002639">
        <property type="entry name" value="UreF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038277">
        <property type="entry name" value="UreF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01730">
        <property type="entry name" value="UreF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009467">
        <property type="entry name" value="Ureas_acces_UreF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3" id="KW-0996">Nickel insertion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="21192" checksum="787838B0126AA173" modified="2014-01-22" version="1">MLLVLADGRLPSGGHAHSGGLEAAIASGRVRDVAQVGEFLRGRLATSGLVAAAFAASACARTGRWLDLDGGLDARTPSPALRKASRAQGRALMRAGRAMWAVPDIGREPHHPVALGVLAHAAGLGPLEAAVAAAHGTVTGPASAAVRLLGLDPYAVHAVIAGLAPDIDRVAADAAARVHDPVDDLPAAGAPLLDIGAELHATWEVRLFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W679</accession>
    <name>U5W679_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MarR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560957.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31820"/>
    <dbReference type="EnsemblBacteria" id="AGZ44624">
        <property type="protein sequence ID" value="AGZ44624"/>
        <property type="gene ID" value="AFR_31820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6441"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_22_2_11"/>
    <dbReference type="OrthoDB" id="2016088at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="154" mass="16719" checksum="0B73D0D539F0227D" modified="2014-01-22" version="1">MERVTDVAAAVESTVESLLGVFETARLAQSPAVPPAQLRVLTIISRSQHTNMSRLAEALDVVPSSASRLCDRLEATGLLRRVPDPRDRREVRLLLTPAAGRLLDELRERRRAALAEVLERMSPARRADLVLALEAFDIAAGTEADGDADGLRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W691</accession>
    <name>U5W691_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH luxR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560972.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31895"/>
    <dbReference type="EnsemblBacteria" id="AGZ44639">
        <property type="protein sequence ID" value="AGZ44639"/>
        <property type="gene ID" value="AFR_31895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6456"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_2_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="818"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="885" mass="94160" checksum="5C82CA8F2F3D941A" modified="2014-01-22" version="1">MGLVGRVPEVVALDRLRAAAAAGEGGAALLVGEAGMGKTTVVEEAVARATAAGATVATGRAVPDEGAPAYWPWLRLIEDLPGLPPELLTAATAAGESPAAARFRVAQRTVRALRETGHLVLVLEDLHWADAGSLALLRLLSRELPGSRLLVIATARPPFPLDDVPGAEVLRLEPWEPAAVGAYLGPAAHASWTPVVHRLSGGNPLYVRELTRQLSREDRLRRPAGGVGVPTELRRLVGRRTAQLGAACRDLLGGAAALGAEIDVAVLRAAAPEPAAVDDLLTEALDAGVLTDDPWRPALLRFAHDLVRQARYDDLARAERIAWHHRLADALADAGAAPAEIARHRVRCAVDAESRQAAVAACRAAAAAALDFAEAIRWHDHTIDLADSPADRLARALASYADGRLDHAIADCTAVQRAGVPTLAVEAALVVRGVAGAHGPALEVLCERALALAGRHPRVLAQYAYLLAERGDVRRAGEISAEAMTLAEESGDPDDVAAAVHARHQIVDPFDQPEVVLALADRSCSLDRPDAELWGRTWRIDMFLMRGDTTAVEQETARLSTLADRLGWPLARWHLLRARAARSLLAGRFTEAEQLALEARDLAVRTQDGAGAQLFYAFGGGLALHTGRSEQYLGRAAEWLTSLLTVPIAAAQLGRTAMDAGDTTTVELCWQNLRPILAGLPPDGRRTYVLITSGELAAWLGDLESARKCYDRTVRYEHIHLNNTTSCYGATARSLGTIASALGDHDAAVRHLTTAVTMDASSPPFEAHAKLGLAQALISRAAPGDRRRAQDLATEAATAARRLGMPPVATAAQTLADKTSGAGALTTREREIAHLVAEGHPNRTIAADLVLSERTVETHVRNLLAKLGLTNRTQIATWIRTTSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W697</accession>
    <name>U5W697_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type cobalamin/Fe3+-siderophore transport system ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_32275</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561045.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32275"/>
    <dbReference type="EnsemblBacteria" id="AGZ44713">
        <property type="protein sequence ID" value="AGZ44713"/>
        <property type="gene ID" value="AFR_32275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6533"/>
    <dbReference type="eggNOG" id="COG1120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="28697" checksum="F1B88E2DC89AD377" modified="2014-01-22" version="1">MTSRLSGSDLTLAYDNRVITSALDVTIPDGSFTVIVGPNACGKSTLLRALSGLLAPKAGSVVLDGAAINSYRAKEVARRLGLLPQTATAPEGISVADLVARGRYPHQKLLRQWTPEDENQVAAAMEATAVTALADRAVDELSGGQRQRVWMAMALAQQTNILLLDEPTTYLDIAYQIDLLDLCADLNEEGRTLVAVLHDLNQACRYATHLIAMKSGRIVAEGNPSEIITAELVEDVYGLVCEVIPDPQTNTPLVVPAARTARAGRLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7K6</accession>
    <name>U5W7K6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_28595</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28595"/>
    <dbReference type="EnsemblBacteria" id="AGZ43980">
        <property type="protein sequence ID" value="AGZ43980"/>
        <property type="gene ID" value="AFR_28595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5798"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="27"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="294"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="389" mass="40074" checksum="D38F5BA6B850776F" modified="2014-01-22" version="1">MKTSRLSVLDLVSLGLVGLRVRRMRAALSALGISIGIATMVVVMGIPASSGQALSRDLALLGTNLLQASAVPDQQPPVAFDEHAAAMAARIAPVTAASAVANTHSTIARNERADNSAATTVLAADEGILTLLDGTVRAGRWFDPTFPTVVLGAEAAERLGIQEPAGGPQLFVGGRWFTVTGILDPVPLAPEIDRAALVGWPAARRELGFDGRPTVVYVRSHEPSVEAVREVLPATVLPNAPGMVRVTRPSDALAAKRTTDTTFSALFLGLAGVALLVGGIGVANTMVISVLERRREIGLRRALGAHRGQIRTQFLAESVALSLLGGIAGTVLGAAATVAYALHQHWPPVIPPAALAAGTGGALLVGIVAGVYPSMRAARLTPTEALATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W870</accession>
    <name>U5W870_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561680.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35440"/>
    <dbReference type="EnsemblBacteria" id="AGZ45344">
        <property type="protein sequence ID" value="AGZ45344"/>
        <property type="gene ID" value="AFR_35440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7172"/>
    <dbReference type="eggNOG" id="ENOG5032C9H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500440_0_0_11"/>
    <dbReference type="OrthoDB" id="1776054at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665656" evidence="1">
        <location>
            <begin position="28"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="184" mass="19407" checksum="D3E7AC8CAD891B15" modified="2014-01-22" version="1">MRSRLFALGAAVALLALAGCTDKEADAATLPPVQIVQQPAASAGGACILWDYAFIEEKLGVKFNVAAADQIDDTSTCVVQTERDDWPYLALSVVESTKADADLFTEELTPKKATKVKGLGKAGYRLISKATGGHGPTVEIGWLSEAAQLQTLRFTFAKEASGDDVKQMSPKLLSLAKAMDTTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8B6</accession>
    <name>U5W8B6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29525"/>
    <dbReference type="EnsemblBacteria" id="AGZ44166">
        <property type="protein sequence ID" value="AGZ44166"/>
        <property type="gene ID" value="AFR_29525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5985"/>
    <dbReference type="eggNOG" id="COG0433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011350_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="PTHR42957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="58"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="659"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1047" mass="114040" checksum="9CE79E3DA74682A0" modified="2014-01-22" version="1">MIGDGEKRALIELRRLDWAPTAEDVWTSLDIHLDSLNGTAGAAVLRAFDDAGVNRSASPLGLVIEGQHGSGKTHLLRWAREKVQLQHGYFFLMGVADGRSFWVNIVNTFLRGLRRSGSYRHSQLAVLLERLSERARMPLALRQQVRGQAPLSPTTLDTFAAEFRRAEPDAGRECRQTLRALVLLASSDGNVADLGESWLLSLPDLAPADLAHWGLPAHPKNAQDVAQEISRLLACTGASMLAVDQIDTIFAQSRPETAGQRAADPVHRVTATMTLAEDLGNGLMDLREKLFRTVTVVACLPMSWDLIKKQALVSVGGRFRQETRLSRLPSREVAAELISVRFAPLFEVANFKPPYATWPIRPEALTDALDYTPRQLIERADEHVRYCLAHNEVVPLTNLHQHRLHVELAVPQIDPGKLAAFDAHFSQLRTEATVDGALQPKTEDTEMSRLLEAGLRAWTIEQGDRASRYRVQPGEQGSPSAHAWLRELLNKETEDEAHWSFRGLSHSHHRAVQARMARLRGFVNLDPAVSKRRAILLRNVPWPNGPVSRRVLDDFLDVGGVVTDVSSDDLRTFAALAELLTENDAALADYLRTRQPASRTTLFVKVFGAPPGPPAPTPDPPTGGSPSSAADVPTAQDDSIPLGAVTETGQPVWVPLESLRKHAVIFAGSGSGKTVLIRRLVEESALLGVSAIILDPNNDLARLGDAWPEPPTSWREDDPAKADNYLTGTDVVVWTPRREAGRPLSLQPLPDFSAVRDEPDEFALALDAAVATLAPRARMAANTAKADRGRAVLRQALEHFGRDDGGGLDEFVELLSDLPDEATSLTRAREVAADMAETLKAAMVNDPLFGGAGTPLDPGVLLTPRPGRRSRVSVIGLIGLPSDEQRQSFVNQLQMALFAWIKRNPAGDRPLGGLFVMDEAQNFAPSGAMTACTESTLALASQARKYGLGLVFATQAPKGIHNRIVGNAATQFYGFLNSPTQIAAANEVARAKGSAVLDISRLRAGQFYAVTEGKPFRKVSTPMCLTYHPSSALTAEEVLLRARREGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8P9</accession>
    <name>U5W8P9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560653.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30280"/>
    <dbReference type="EnsemblBacteria" id="AGZ44316">
        <property type="protein sequence ID" value="AGZ44316"/>
        <property type="gene ID" value="AFR_30280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6131"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_1_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="30"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="30084" checksum="2CBB78AD569B5FF5" modified="2014-01-22" version="1">MTDTEFGPAVRRWRDRVTPEAAGLPAGGHRRAAGLRREELALLAGISVDYITRLEQGRSTGPSAQVVEALARALRLDATEREHLFTLAGLAPPARGVVSAHITPGVQRLLDRLSDTPVAVHDAAWNLITGNPLWAALFGDPAALHGHERNIVWRHFAGFNSRVIQTPEQERVFGAATAADLRAATARYPADSRVTDLIKELLARSETFARIWEAGAITPHDSTSKLVQHPQVGDIHLDCDVLTVPGSDLRIVAYTAEPGSEAADRLALLSVVGQQSFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8R6</accession>
    <name>U5W8R6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561743.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35755"/>
    <dbReference type="EnsemblBacteria" id="AGZ45407">
        <property type="protein sequence ID" value="AGZ45407"/>
        <property type="gene ID" value="AFR_35755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7237"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_6_2_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019952">
        <property type="entry name" value="F420_OxRdatse_Rv1855c_pred"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03560">
        <property type="entry name" value="F420_Rv1855c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="33197" checksum="6E4479A0C0D75785" modified="2014-01-22" version="1">MRLVIFTEPQQGASYDDLRRVAQLAEETGYDGFFRSDHYVAMGGDGLPGPTDAWLTLAALAVQTSTIRLGTLVTSATFRLPGPLAISVAQVDAMSNGRVELGLGGGWFEREHEAYGIPFPPLGERFDRLEEQLQIIEGLWTTPAGQAYDFAGKHYQVINSPALPKPTQSPRPPIIVGGAGKKRTPALAARFADEFNAPFAKLEDIPAMFDRVRAASTAIGREQPPALSAAAVLCVGRDDAEVKRRADAIGRDVEEMRGNGGIVGTPDQAVEILGRYAEAGATRMYLQQLDLSDLNHLELVATAVAPQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Y8</accession>
    <name>U5W8Y8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561920.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36640"/>
    <dbReference type="EnsemblBacteria" id="AGZ45584">
        <property type="protein sequence ID" value="AGZ45584"/>
        <property type="gene ID" value="AFR_36640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7412"/>
    <dbReference type="eggNOG" id="ENOG5033N6W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2068094_0_0_11"/>
    <dbReference type="OrthoDB" id="1728516at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="13250" checksum="865C30150345FD91" modified="2014-01-22" version="1">MSTVSWSSFTGDQVAALAQGESFLAAPGERDCPACGSHSVRAYVNAPENARRPTLVSYVWCRSCHRFVGTRAKHPEGLVFSDPLALLSAAERRELERSLVGFLAHLDELWESGALPQTFTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Z2</accession>
    <name>U5W8Z2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cyclophilin type peptidyl-prolyl cis-trans isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_31025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560800.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31025"/>
    <dbReference type="EnsemblBacteria" id="AGZ44465">
        <property type="protein sequence ID" value="AGZ44465"/>
        <property type="gene ID" value="AFR_31025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6280"/>
    <dbReference type="eggNOG" id="COG0652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_8_1_11"/>
    <dbReference type="OrthoDB" id="1861282at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="3">
        <location>
            <begin position="113"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="29830" checksum="5B0A91531C25C7B4" modified="2014-01-22" version="1">MASSRDRARKLAREKLDRQQARRAGQQRRRRQIQAGLGAALALVLIVAGVAWLGGAFDNDEPVDTTATDQCAWTPQNAESNPDLKDVGEPPTSGLPTEGTRPMTITTDQGEPIVVSLDLEQAPCSAADLTYLASKKFYDNTTCHEITTEGAIRCGDPSGTGQGGPAYSLYNENTPLAPTPSASASPSPAAPLYPRGTVALIGNPPGANGSQFLIFFKDFTPKTEPQYPIVGTVTGGLATVTKLGKIATVANTAGDKVTPKDKITIKSLTVGEPGAAPAAVPSASSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9B0</accession>
    <name>U5W9B0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45793">
        <property type="protein sequence ID" value="AGZ45793"/>
        <property type="gene ID" value="AFR_37695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7623"/>
    <dbReference type="HOGENOM" id="CLU_2152913_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="112" mass="12195" checksum="FC4EFBF5D196B25B" modified="2014-01-22" version="1">MSSLPDAERDADEVVQILAGLAALLRRREVVLDDDTRTMIGLAVDGGVSATRVLEYLRALQGAGDGVAAAVPQRLGPWRDWVPPRGNLFGELAGAPRIEEMPRRDRRHDPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9G1</accession>
    <name>U5W9G1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560943.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31750"/>
    <dbReference type="EnsemblBacteria" id="AGZ44610">
        <property type="protein sequence ID" value="AGZ44610"/>
        <property type="gene ID" value="AFR_31750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6427"/>
    <dbReference type="eggNOG" id="ENOG5031VG3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193477_0_0_11"/>
    <dbReference type="OrthoDB" id="2048977at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="64" mass="7027" checksum="EFCD54CD5287B950" modified="2014-01-22" version="1">MQIRVDYAVNKVMQTHRGQSEDAVAQAIQDQLRSVGVVPNGRQIAQYAAVISALPLLPPNNRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA36</accession>
    <name>U5WA36_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-lactamase2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32690"/>
    <dbReference type="EnsemblBacteria" id="AGZ44796">
        <property type="protein sequence ID" value="AGZ44796"/>
        <property type="gene ID" value="AFR_32690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6615"/>
    <dbReference type="eggNOG" id="COG2367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050510_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="PTHR35333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase2" evidence="2">
        <location>
            <begin position="146"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="33433" checksum="65535E7960ECFEB6" modified="2014-01-22" version="1">MRRTQLWIFAVAALVIVGGVSLVGLGMQDGGSAGGGGGVLPRSLTFGDDSSPTPKASPTPTGPSPEELAARERAKRVKALDAALKKYAKSSPEFSVAVLDKKTGERYSYRGSEKYDTASIVKVQVLACMLLQAQDDDRDLSSSQLALAKRMIRLSDNNATTSLFVQLGKVSAVSKCNKRLGLKQTVVSSSWGLTRTTVDDQVRLLSELVDTKGPLDKDSRKLAFTLMNTVDDTQDWGVPSVAKSGETTTVKNGWDTRSADGGLWAVNTVGRITADDDVDISVAVLSHNNQSMDGGITLVQKVAKMTRQYLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA64</accession>
    <name>U5WA64_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562252.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38315"/>
    <dbReference type="EnsemblBacteria" id="AGZ45917">
        <property type="protein sequence ID" value="AGZ45917"/>
        <property type="gene ID" value="AFR_38315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7751"/>
    <dbReference type="eggNOG" id="COG5282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031872_1_1_11"/>
    <dbReference type="OrthoDB" id="991598at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018766">
        <property type="entry name" value="Zinicin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042271">
        <property type="entry name" value="Zinicin_2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39420">
        <property type="entry name" value="PTHR39420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10103">
        <property type="entry name" value="Zincin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03624">
        <property type="entry name" value="TIGR03624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="411" mass="44353" checksum="B0AD40D4554FC8FE" modified="2014-01-22" version="1">MPDIPFGFSLPGAQPPDPSDPQQMQQFMNQLQQMFAAPGSGPVNWDLARQVAASQLSQTGDPAVNMYEHNAVSEALRLADLWLDPASALPSGIRDATAWNRNEWVYNTLEVWKKLCDPIAGRMVGAMGDLVPEEARAQLGPMQSMVATLGGALFGGQLGQALGQLAAEVLSAGDIGLPLGPAGTAALVPANIKAYGDGLELPEDQVRLYVALREAAHQRLFQHVPWLRAHVLNAVETYANGITVNREAIEEAMSRVDPSDPESMQQMALEGIFTPEDTPQQKASLARLETALALIEGWVGHVVDSAAGDRLPSVSALGEAFRRRRAAGGPAEQTFAALVGLELRPRRLREAGALWAAVTEHRGVAGRDALWDHPDLLPTDEDFADPQKFAQAKSDWDISELENLGKEEDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-02-10" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA78</accession>
    <name>U5WA78_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_38390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38390"/>
    <dbReference type="EnsemblBacteria" id="AGZ45932">
        <property type="protein sequence ID" value="AGZ45932"/>
        <property type="gene ID" value="AFR_38390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7766"/>
    <dbReference type="eggNOG" id="COG2816">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_0_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015375">
        <property type="entry name" value="NADH_PPase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09296">
        <property type="entry name" value="NUDIX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="5">
        <location>
            <begin position="171"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="35349" checksum="65B36FB3B23B7DC1" modified="2014-01-22" version="1">MSQNEEQPDVGEQPGLDGPPLARTSLDRAAHHRRDEDWLAQAWKTGLVVVIDIAQGGRALITGRTGDVPRLLLVSADAAPEGERLFLGIDPAGVPIFAVAAPLPEAAGAEAMTLRDIGDRLDPRDAGIFTTAAALGNWHASHLFSPRNGEPTTVTEAGWSRTDPDGRQMWPRTDPAMIVLVHDGVAGPEGRCLLGHNAAWPEVGGVRRFSCLAGYVEPGESAEAAVVREVREEVGVRVRSLQYEGSQSWPYPGSLMLGYTAVANREQAITVDPEEIDEARWFTREDIGRMIAGDYVEPASGVRMNLPMRSSIAFYLVERWLGGLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAF4</accession>
    <name>U5WAF4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33340"/>
    <dbReference type="EnsemblBacteria" id="AGZ44926">
        <property type="protein sequence ID" value="AGZ44926"/>
        <property type="gene ID" value="AFR_33340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6747"/>
    <dbReference type="eggNOG" id="COG5395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078522_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018750">
        <property type="entry name" value="DUF2306_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10067">
        <property type="entry name" value="DUF2306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="28273" checksum="7943DFDAF21BCD5B" modified="2014-01-22" version="1">MVAGTIGGMTTTSARNTRNARLVVAGLLLLTAVPVLAGALRIATLSGAGEVTPDKSYMALDPAPVVVHIIGATLFCVLGAFQFHRGFRQRRPGRHRIAGRLAAAGGFAAALSGIWMTLFYPLPHHDGARFGLLGTERILFGTLMAAAIVISFLAIRRRNIVRHRAWMMRGYAIAQGAGTQAVIIAPWIAAFGDTDVVTRAVLMGTAWTLNLAVAEWIIRRRPQHRVPAPQVTTNPAISAGPAISAGPAISAGSPVTAGPAGRRGGMRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAM2</accession>
    <name>U5WAM2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561338.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33715"/>
    <dbReference type="EnsemblBacteria" id="AGZ45001">
        <property type="protein sequence ID" value="AGZ45001"/>
        <property type="gene ID" value="AFR_33715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6824"/>
    <dbReference type="HOGENOM" id="CLU_123724_0_0_11"/>
    <dbReference type="OrthoDB" id="2047663at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="181" mass="20391" checksum="21F91A72F848F067" modified="2014-01-22" version="1">MDVGALYNSITLSVSLTALAMSSVLTVRQLRTARTASQDANITQALLCLNGEYRSDEFRESEDFVLHRLAEECSSDLGVADLPFEARRHVCRVGHFYGDYGMLAALPTYDQARILGCVHYRVREAWSVLEPFAVAERQVRGNSYWSYFENLAFLATKVDKQQVLDSQGLRRFDGARWPSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAX7</accession>
    <name>U5WAX7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_40595</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562705.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40595"/>
    <dbReference type="EnsemblBacteria" id="AGZ46373">
        <property type="protein sequence ID" value="AGZ46373"/>
        <property type="gene ID" value="AFR_40595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8216"/>
    <dbReference type="eggNOG" id="COG0643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000650_5_2_11"/>
    <dbReference type="OrthoDB" id="305000at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004105">
        <property type="entry name" value="CheA-like_dim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037006">
        <property type="entry name" value="CheA-like_homodim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037257">
        <property type="entry name" value="T2SS_E_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02895">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01231">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160246">
        <property type="entry name" value="SSF160246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPt" evidence="7">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="267"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="6">
        <location>
            <begin position="478"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="6">
        <location>
            <begin position="634"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="129"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="3">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="779" mass="83720" checksum="739545B1DE1C53E4" modified="2014-01-22" version="1">MEDLGEIVGEFLMESHENLDQIDRDLVALEQEPGSRDLISRIFRAIHTIKGTSGFLAFSRLETLAHAGESLLSRLRDGVQPVTPTTITTLLATIDGVRSLLEAIEQDGNEGDVDVDSIITAVHAQMNAPAETAAAPAAPAEPAETPEPLEEKAVRPAPEPVEGQRKPLGEMLVESGAAAPSDVGSALQQQLEGDERKLGAILLEEGKAAPAAVTEALQAQTPKRSVADSAIRVDVDLLDGLMNLVGELVLARNQLVRGVMETGDSGLVRSAQRLGMITSELQEGIMKTRMQPIEHIWSKLPRVIRDLSQSLGKKVELVMEGKETELDRSLLEAVKDPLTHLVRNAVDHGIEEADKRVAAGKSPEGTLTLRAYHEGGHVAVDVADDGAGMDVNRIAQKAVEKGLLRADQVAGMDKREIMAMVFQPGFSTAAKVTNVSGRGVGMDVVKTNIERIGGAVSVDSTLGEGTVWRLTIPLTLAIIQALTVDCGDQRYVVPQVAVLELVFIDGQSTKIEYASGAPVYRLRGKLLPLVRLDRALGLDVGGDQGVYIMVLHADGRRFGLVVDRVLNTEEVVVKALNSRFKDIGLYAGATILGDGKVGLILDVSSLARRSHLTAESDRENLVATSRAAGAAGTGERLLVTAVGERRVAIPLDAVTRLEEFPRDRIEHAGSREVVQYRGQILPLVRLSHLLGAYSEVEESDTVSVVVYSEGERSVALVVDRIVDIAENQTTARREAEEDGLVGTAVIQQRVTELLDVRRAILAADPNFYTEAADEILVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB61</accession>
    <name>U5WB61_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amylovoran biosynthesis glycosyltransferase amsB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40995"/>
    <dbReference type="EnsemblBacteria" id="AGZ46453">
        <property type="protein sequence ID" value="AGZ46453"/>
        <property type="gene ID" value="AFR_40995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8299"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_0_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="34555" checksum="3F35D4F3C19190A5" modified="2014-01-22" version="1">MSVVIPTRFRPDLVLRAVTGVLAQTMDDLEVIVVVDGPDEDTVTALAALPDPRLRVLVQPKKGGAPHARNTGAQAARGRWTAMLDDDDEWLPGKLEVQLKLAYSARRATPIVASRLVNRTPRAESIMPRRLPDPDEPVSEYLTVRRGLFYGDGFIQTSTILAPTELFHRVPFTVGLRRQQELDWTLRAVRHEDVELIVAPDALVVWHQDENRERISLEMPWRQQFEWSQRSRELFTPRAYAAFLMSVVSSMAAPTRSAKVFRELLGEARTHGRPGAIDYLTFMQIWALPAEVRHALRDKVLGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBG1</accession>
    <name>U5WBG1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="4">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_35320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561656.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35320"/>
    <dbReference type="EnsemblBacteria" id="AGZ45320">
        <property type="protein sequence ID" value="AGZ45320"/>
        <property type="gene ID" value="AFR_35320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7146"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_0_1_11"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052832">
        <property type="term" value="F:inositol monophosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052833">
        <property type="term" value="F:inositol monophosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="275" mass="28344" checksum="4B75206E356275FF" modified="2014-01-22" version="1">MENAAERQVSTPGELLGIAVRIAREAAETARRMRVDGVTGVRTKSTDTDVVTAADRAVERQVVAALAAERPQDGVLGEEYGNSTSAASGAVRWILDPIDGTVNYLYGLPQYAVSLAAEVDGVVVAGVVRNAATGDEWTATLGGGAWRDGRRLSGSVRTTLDQSLVATGFGYDAARRAHQAAVVAGLITRVRDIRRFGAASLDLSLAAEGQVDAYFEKGLNPWDHAAGGLVAAEAGLRVSGLDGAAPGADMVLAAPPALFGPLHDVLTELDAAGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBI9</accession>
    <name>U5WBI9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acyl-CoA dehydrogenase type 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_41300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562846.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41300"/>
    <dbReference type="EnsemblBacteria" id="AGZ46514">
        <property type="protein sequence ID" value="AGZ46514"/>
        <property type="gene ID" value="AFR_41300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8358"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_2_11"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013107">
        <property type="entry name" value="Acyl-CoA_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08028">
        <property type="entry name" value="Acyl-CoA_dh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="3">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="2">
        <location>
            <begin position="121"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_2" evidence="4">
        <location>
            <begin position="241"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="385" mass="40199" checksum="C116945841055DF7" modified="2014-01-22" version="1">MTAEEARRLAPRFAARAADHDRDGSFPADDFADLRRSGLFGLMVPERLGGAGAGFADYAAIAYELARGNGATALVFNMHASVTGALGSVTNELADALGLPPEALEARDNYLRAAAEGSWYAVAMSERGAGSRLSQLTTSYERTADGFHIKGSKTFCSGSGHADAYLVAARSADDPAQVSQFLVPAGDGLTVEPTWDALGMRATGSHDVHVDVTVPETALLGGVEGLALVVAQLAPHWMVASYAAVYVGVARAAVDAAVEHVNARNLGHLPAVRARIGRADAAVAAAHLTVMEAARRVDENPGDTETNRWVWRAKLLAGTTAAEVAASMLEAAGTSAMRRGHPLERLYRDARAGSLQPATSDVCADWLGVEALGGDPDSQGSAPRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBK8</accession>
    <name>U5WBK8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_41400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41400"/>
    <dbReference type="EnsemblBacteria" id="AGZ46534">
        <property type="protein sequence ID" value="AGZ46534"/>
        <property type="gene ID" value="AFR_41400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8378"/>
    <dbReference type="eggNOG" id="COG1565">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046703_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PTHR12049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="35917" checksum="7DB068A37A522BA1" modified="2014-01-22" version="1">MNNCTAYIDSVTTVGWRSAMTRALYGPGGFFTRAATGPADHFRTSVHASPLFAGALLRLIQEVDDALGHPDHLDVVDVGAGRGELLTALDALVNGSDLRERVRLVGVELAGRPDGLPKEIGWRADIPDSVTGVLLATEWLDNVPLDLAEVDADGRLRQVLVDPDTGLETLGRDLDAADRFWAARWWPDAGLGDRIEIGWPRDAAWAEAVSSVRRGCALAIDYGHLRSSRPPLGTLTGFRDGRQIEPVPDGSCDVTAHVAIDSAAVAAGTAYRIIGQREALRGLGVDGGRPPLALASTDPAGYLRALSAAGAAAELTDPAGLGGHWWLLHEVGIELRGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBR0</accession>
    <name>U5WBR0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562881.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41475"/>
    <dbReference type="EnsemblBacteria" id="AGZ46549">
        <property type="protein sequence ID" value="AGZ46549"/>
        <property type="gene ID" value="AFR_41475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8392"/>
    <dbReference type="eggNOG" id="ENOG503498K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151322_1_0_11"/>
    <dbReference type="OrthoDB" id="1918051at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="9922" checksum="A362B1F2334380BE" modified="2014-01-22" version="1">MIATELVAQVAAALQQGTLAAPEPKGINTEGVVTFFASNIAPILLAILGIIFIGRASRGEISKVLTSSAIAIVGLAFIAGAATLFFVGDYLISLIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBR2</accession>
    <name>U5WBR2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Riboflavin biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="11">Riboflavin kinase</fullName>
                <ecNumber evidence="11">2.7.1.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">Flavokinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="11">FMN adenylyltransferase</fullName>
                <ecNumber evidence="11">2.7.7.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">FAD pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="11">FAD synthase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_35920</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + riboflavin = ADP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ribF family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="11"/>
    <dbReference type="EC" id="2.7.7.2" evidence="11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561776.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35920"/>
    <dbReference type="EnsemblBacteria" id="AGZ45440">
        <property type="protein sequence ID" value="AGZ45440"/>
        <property type="gene ID" value="AFR_35920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7271"/>
    <dbReference type="eggNOG" id="COG0196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048437_0_0_11"/>
    <dbReference type="OrthoDB" id="1504821at2"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02064">
        <property type="entry name" value="FAD_synthetase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015864">
        <property type="entry name" value="FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002606">
        <property type="entry name" value="Riboflavin_kinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="PTHR22749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06574">
        <property type="entry name" value="FAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004491">
        <property type="entry name" value="FAD_Synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00083">
        <property type="entry name" value="ribF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 11" id="KW-0288">FMN</keyword>
    <keyword evidence="11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavokinase" evidence="12">
        <location>
            <begin position="183"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR004491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ45440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="33878" checksum="5192D76839DD3081" modified="2014-01-22" version="1">MQRWRGYEAVPGGFGRSVVTIGVFDGVHRGHQAIIGHTVKRARDLGLQSVVVTFDPHPAEVVRPGSHPAVLTEPVRKAELIEQLGVDALCVVPFTPAFSQLTAEEFVHDVLVEALHAAVVVEGDNFRFGHKAAGDLTMLTALGRTFGFTVEEAPLVAEEGLIFSSTYIRSCVDAGDVAAAAAALGRPHRLGGVVVRGDQRGREIGFPTANLMCHRYAAIPADGVYAAWLIRGGKNGGPLPASVSVGTNPTFNGRERRVEAYVLDFAGDLYGERVSLDFVAHLREQRVYTGIEPLVAQIREDVEETRRVLARPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC52</accession>
    <name>U5WC52_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_36520</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561896.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36520"/>
    <dbReference type="EnsemblBacteria" id="AGZ45560">
        <property type="protein sequence ID" value="AGZ45560"/>
        <property type="gene ID" value="AFR_36520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7388"/>
    <dbReference type="eggNOG" id="COG5542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036370_0_1_11"/>
    <dbReference type="OrthoDB" id="1297390at2"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:glycolipid mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007315">
        <property type="entry name" value="PIG-V/Gpi18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468">
        <property type="entry name" value="PTHR12468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04188">
        <property type="entry name" value="Mannosyl_trans2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="343"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ45560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="392" mass="41072" checksum="CF1AE2E7E7A31BD1" modified="2014-01-22" version="1">MSVTAEEAGAEAPTVAGRLWDRAGRWHGVGLAVGILALTRVVQLLLLVGLDRATEEPLGLRANLLLWDGGWFLRVAEGYPSGYTYGADGTLQGNELAFFPLYPMLIRGLSTLGLEPGFAAITVSWLASIGAAVALHLLGTSLYGRRAGWALVAICCAAPMSVVLSMAYSESLFLALVAGMFVAAHRQVWWAAGLLGLGAALTRPTGAAAAVALAVAAILAVRRDPSKKWPPLAAAAVALAGVPAYLGWVAWRVGDPEAWFKIQTAGWGTSFDFGASTLSFLGSTLTLGEGWVPMSVALILLAAVAAAWVALAGRPWLPLAVYGLVAMVLAYGQAGFYHSKPRLLLPVLLTLLPSVVAAARARPRVAVLAIAAWAAFGLWYGAYLVAVWPYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC77</accession>
    <name>U5WC77_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pas/pac sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_42525</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563090.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42525"/>
    <dbReference type="EnsemblBacteria" id="AGZ46759">
        <property type="protein sequence ID" value="AGZ46759"/>
        <property type="gene ID" value="AFR_42525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8606"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_660458_0_0_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="170"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="268"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="484" mass="51934" checksum="9EEE8DBACCB65257" modified="2014-01-22" version="1">MPDRPDYAALTRGQIALLDRINAGEAGLAVLNQLVQLAQDVLGGKGAGFAEYSPGHGRIIAGSGVCERAVGRRVDREDTRLTEGNRTLVISLDSLNDEFASQIEGGDLHRMLGARCEIGGLIVGSLHVYYGEDAGEPTAEHHAVLELLAANVGHMYGDQAGLPVHGDGPVVAALSDGLAIVDREGIVQLWNPAAEQLTGSSAAEVLHRALQFPVPPPGQVLVHRLPSGRWLKVTSGDLPHTPAMRVVTFRDSTDQDRRHEDRDLFVAVTSHELRTPVTVIKGYADTLTNHWESLGEDGRREAVRVIGARAGELARLVDRLLSATSDDGEVGGSPPGPFDLVDGLRAAAGELPADLRRRLVLDQLPGELPKAFGDRDSLATIVTELATNADKYSAPETPVEVTAAADEATVTFRVADRGIGVKPEHVERAFERYWQGDSTDRGRNPGAGLGLYLVRRIVERQHGWVSLRPREGGGTVAEVRLPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC82</accession>
    <name>U5WC82_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative fumarylacetoacetate hydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561936.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36720"/>
    <dbReference type="EnsemblBacteria" id="AGZ45600">
        <property type="protein sequence ID" value="AGZ45600"/>
        <property type="gene ID" value="AFR_36720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7428"/>
    <dbReference type="eggNOG" id="COG0179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_4_2_11"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018833">
        <property type="entry name" value="DUF2437"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10370">
        <property type="entry name" value="DUF2437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2437" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="63"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28612" checksum="881432242A2275D9" modified="2014-01-22" version="1">MRFARFVHAGGVSFGVVEGESGAGTQGLTIAEIGSLPFEQVRFTGQRWALADVRLLAPIFSSKVIGIGRNYADHAAELGNEVPKEPLLFIKPSTSVIGPNDAIRIPPQSKQVEHEAELAVVIGATGARRVDRAGAEKAIFGYTVGNDVTARDLQRSDPQWTRAKGFDSFCPIGPWIETTLDVSDLEVRCEVGKNPDAMEVRQLGRTKDMVFDVPTLVSYVSHVMTLLPGDIILTGTPAGVSQIFAGETVSVSVQGIGELRNPVVSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCG1</accession>
    <name>U5WCG1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cellulose-binding family II protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_42015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562988.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42015"/>
    <dbReference type="EnsemblBacteria" id="AGZ46657">
        <property type="protein sequence ID" value="AGZ46657"/>
        <property type="gene ID" value="AFR_42015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8502"/>
    <dbReference type="eggNOG" id="COG3469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019399_4_1_11"/>
    <dbReference type="OrthoDB" id="1081028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666298" evidence="2">
        <location>
            <begin position="26"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="3">
        <location>
            <begin position="32"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="4">
        <location>
            <begin position="180"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="471" mass="48066" checksum="AFF1859E0E748E96" modified="2014-01-22" version="1">MARTRLRLSIYVAAALVAVGGGAVAAVAATTPATKAGGLTATFSKDSDWGSGYQARYTIKNTGGAPVSGWQLAFGLPASAKLGSFWDAAVTTAGGVSTAKDRGYNATIAAGSSAEFGFIVAGSGSPTSCTINGVVCGGGSAPTTAPISKPPTAPPTTAPTTRPPTTAPPTTTAPPAPSGSVLVAPYVDMGLLSNGSTTLAQLATSGNVKSYTLAFVTSAGCKASWFNAFDPRSKQFGDQITALRAAGGDVKVSFGGATGIELAQACTDVKALQAEYQAVVSAYNLKYIDLDIEGAAVADPTTIARRSTALAALQKANPGLKISLTLPVLPEGLTNDGLNVVKSAKNAGVDLDLVNIMAMDYGRSAQDYGDLAIQAVKSTKDQIRAIYGNSDAAAFKMVGVTPMLGKNDDNGTFSQSDARDLVAFANSNHVGFVSFWETNRDRNACTGALFQCTNVAQAPFEFSKIFATFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD40</accession>
    <name>U5WD40_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562275.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38430"/>
    <dbReference type="EnsemblBacteria" id="AGZ45940">
        <property type="protein sequence ID" value="AGZ45940"/>
        <property type="gene ID" value="AFR_38430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7774"/>
    <dbReference type="HOGENOM" id="CLU_1631825_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="162" mass="17352" checksum="956290D915D9892F" modified="2014-01-22" version="1">MGTQETTAIKIGLHQVTDLRWPKPVVTGAGKREVTSVPLPRLPWSSTAIEVPMGETAARAVERVTGLRKIFGYGVAPVVILLFVVADVLLLWGRLGDLRPPGWIFAAMGVAGVILILTGLIPDAVARATGAPYVSRGHLRFSKANQDVVNQLIKLNPKVTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDT0</accession>
    <name>U5WDT0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="5 8">5.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_39630</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="5 8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562513.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39630"/>
    <dbReference type="EnsemblBacteria" id="AGZ46180">
        <property type="protein sequence ID" value="AGZ46180"/>
        <property type="gene ID" value="AFR_39630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8017"/>
    <dbReference type="eggNOG" id="COG1087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_10_11"/>
    <dbReference type="OrthoDB" id="1597849at2"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ46180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="325" mass="34502" checksum="BAAD72BF0E4AB8A3" modified="2014-01-22" version="1">MKLLVTGGAGYVGSVTSRLLLDAGHEVTVLDNLSTGFREAVAPDATFVQADISEAGSVLTPGSGFDAVLHFAGLIAAGESMAKPELYWDHNVVRSLALLEAIRAAKVPRVVFSSTAAVYGNPTELPITEGAVKAPTNTYGATKLAFDFALTSETFAHGLAAVSLRYFNVAGALIRPDGTAIGERHDPETHLIPITLQAAAGKRDKLQLFGDDYPTVDGTCVRDYIHVQDLARAHLLALDAATPGEHKIYNLGNGNGFSNRQVVEAAREVTGADLPIEIAPRRDGDPATLVAGSQRARDELGWVPEKNTLTDMIGDAWAFYRSHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDU2</accession>
    <name>U5WDU2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative alpha-L-arabinofuranosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_39680</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 51 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562523.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39680"/>
    <dbReference type="EnsemblBacteria" id="AGZ46190">
        <property type="protein sequence ID" value="AGZ46190"/>
        <property type="gene ID" value="AFR_39680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8028"/>
    <dbReference type="eggNOG" id="COG3534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017810_1_1_11"/>
    <dbReference type="OrthoDB" id="620389at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046556">
        <property type="term" value="F:alpha-L-arabinofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046373">
        <property type="term" value="P:L-arabinose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010720">
        <property type="entry name" value="Alpha-L-AF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06964">
        <property type="entry name" value="Alpha-L-AF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00813">
        <property type="entry name" value="Alpha-L-AF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-L-AF_C" evidence="4">
        <location>
            <begin position="291"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="454"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="503" mass="55311" checksum="E8D4A85F70D1744B" modified="2014-01-22" version="1">MELARLTLDPAFAIGPVDRRLFGSFVEHMGRCVYTGIFEPGHPAADDEGLRGDVVDLVRELGVSLVRYPGGNFVSNYRWEDGVGPVGDRPRRLDLAWRSLESNAFGLNEFMRWSERAGVEPMMAVNLGTRGIAEAAELVEYCNVPDGTAAADLRRKHGVAQPHDVKLWCLGNEMDGPWQIGSMTAHEYGRLATRAGQAMKRVDPSIELVACGSSNADMPTFAAWEATVLELAYDQVDYVSLHHYYDPEKHDEASFRASAVNLDGFVDDVVATADHIRAKLRRSKRINLSLDEWNVWYQSRFTEPEDRGIEDTPALIEDTYTVTDAMVVGNLLISMLRHADRLTVGCLAQLVNVIAPIRTEPGGPAWRQTIFHPFAHTARLARGTVLRAGIQSPTMPTGRYGDVPAVDAVATIDENSGQVVVFAVNRGTEPVALELDLRAFPRVRAGERISIGDDDPAAVNSAAAPDRVCPRTEKMPDLDGGRTTVVLPPVSWHALSFTPEEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE24</accession>
    <name>U5WE24_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_39585</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39585"/>
    <dbReference type="EnsemblBacteria" id="AGZ46171">
        <property type="protein sequence ID" value="AGZ46171"/>
        <property type="gene ID" value="AFR_39585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8008"/>
    <dbReference type="eggNOG" id="COG3845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_92_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="239"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="490"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="515" mass="55093" checksum="7120B8CCE223891B" modified="2014-01-22" version="1">MANDHIDLTVEPGEVHALLGENGAGKSTLMNQLYGLLQPDEGEVLVDGAARVFRSPRDAIAAGIGMVHQHFMLVPVFTVAENIALGAEKTRGGFLDRRRARREVLEVSERYGLPVDPDALVENLPVGAQQRVEIVKALTRDVDLLILDEPTAVLTPQETEELLAVMRGLSDAGKSIVFITHKLKEVKQIADRITVIRRGKTVGTASPDTPEDELAALMVGRAVSLEVTKAPAKPGREVLKISGLIVDDDRGVRAVDSVDLVVRAGEVLGIAGVQGNGQTELVEAVMGLREPRAGTVEVDNEDLIGMSTKQILRSGIGYVPEDRSLDGVVKEFSVAENLVLDQYDRPPYGNAFRLNPAAVGEAARERVEQFDIRTSSPDAAVSTLSGGNQQKVVVARELSRPLRLFIASQPTRGVDVGSIEFIHGRIVRERDVGTAVLVVSSELDEVVGLADRIAVMYRGRILAIVSPDTPREEIGLLMAGITDPSAVAVADDAPGIPEEEPEAGDGDGVAEKGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEB2</accession>
    <name>U5WEB2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative PaaX-like regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562603.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40085"/>
    <dbReference type="EnsemblBacteria" id="AGZ46271">
        <property type="protein sequence ID" value="AGZ46271"/>
        <property type="gene ID" value="AFR_40085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8112"/>
    <dbReference type="eggNOG" id="COG3327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067515_2_0_11"/>
    <dbReference type="OrthoDB" id="1210594at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012906">
        <property type="entry name" value="PaaX-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013225">
        <property type="entry name" value="PaaX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011965">
        <property type="entry name" value="PaaX_trns_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07848">
        <property type="entry name" value="PaaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08223">
        <property type="entry name" value="PaaX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020623">
        <property type="entry name" value="PaaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PaaX" evidence="1">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="PaaX_C" evidence="2">
        <location>
            <begin position="160"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="270" mass="29627" checksum="6F44D0DF7359AD2C" modified="2014-01-22" version="1">MQARSALFDLYGDYLRPRGGRAPVAALVKLLAPVGIAPPAVRTAVSRMVRQGWLHPMRLVSGPGYLLTPKAARRLDEASARIYRTGRNGWDGRFDLVLTNAPVPRREAKRLTFLGYGMLGELAWVAPRAADEIDAVLTDAAVGFERFSASHAAGSPGAAEVVGRAWDLTEIGRAYEEFIEVQRPIVTAVNARSSDEEAYAARFQLVHAWRSFLFRDPQLPASLLPARWPGASAATFFDRHATRLRPPADRFVERCLASVTRGGRVGFSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF17</accession>
    <name>U5WF17_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphate import ATP-binding protein pstB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_41780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41780"/>
    <dbReference type="EnsemblBacteria" id="AGZ46610">
        <property type="protein sequence ID" value="AGZ46610"/>
        <property type="gene ID" value="AFR_41780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8455"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_11"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="21"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="354" mass="38707" checksum="A016171BE202FE1B" modified="2014-01-22" version="1">MSTDSLAGLTMPGDRITRPFLEVKDLTVRFETDDGVVQAVSDSSFSVERGKTLGIVGESGSGKSVTSLAVLGLHRTRSNAKVGGQIWLDDQELVTASDEEVRKLRGGKMAMIFQDPLSAMHPYFTVGAQIVEAYRVHHGVGKKEARVRAIDMLKRVGIPQPEKRYSDYAHQFSGGMRQRAMIAMALVNNPQLLIADEPTTALDVTVQAQILDLIRDLQEEFGSAVIMITHDLGVVAELADDVLVMYGGKIIERGPAAQVFHSPQHPYTWGLLGSMPRLDRDVRERLNPVKGSPPSLINLPGGCAFHPRCPYAGRNGNRSFNEVPVLRGGVHAVACHLPAESRREIFEQEVAPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNK7</accession>
    <name>U5VNK7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkyl hydroperoxide reductase/ thiol specific antioxidant/ mal allergen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357326.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00465"/>
    <dbReference type="EnsemblBacteria" id="AGZ38382">
        <property type="protein sequence ID" value="AGZ38382"/>
        <property type="gene ID" value="AFR_00465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.95"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013730_1_0_11"/>
    <dbReference type="OrthoDB" id="966287at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14951">
        <property type="entry name" value="NHL-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045302">
        <property type="entry name" value="NHL2_NHL_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="594" mass="63445" checksum="9F015E92350B6104" modified="2014-01-22" version="1">MTARVRAPELRGRGWLNTGGRPLTLADLHGKVVLLDFWTFCCINCLHVLDELRPLEEKYGDALVIIGVHSPKFEHERDPEALAAAVERYGVHHPVLDDADMTMWQQYAAKAWPTLSVIDPESYVVASMAGEGHAEGLVRLIDELITTHEAKGTLHRGDGPYVPPVAPDTLLRFPGKVIELPNGNLLVSDSARHSLAELSPDGETLVRRFGTGERGRTDGSAETASFSEPQGLCLLPTGTADYDVVVADTVNHLLRGLRLDTGEVTTIAGTGSPWRSADDDGKALSSPWDLAWYADQVIIAMAGIHQLWWFDPATGRTGVYAGTTVESLRDGKLPDVWMAQPSGLSAAGDRLWIADSETSALRWIEAGELHTAVGQGLFDFGHVDGPAQDALLQHPLGVGALPDGSVLIADTYNGAVRRFDPADGTVSTVDSGLSEPSDVLVTRDGAVIVVESGAHRLVRLAPGVVKTVAGERHRTERPPSPLAPGEVTLDVVFTPAPGQKLDTSFGPSTRLVVSAAPPELLLEGEGTTTELSRRLVLNPDVPKGILQVVAQAATCDADVEHAACHLTRQDWGVPVVVEPGAPSRLPLILRGLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNP9</accession>
    <name>U5VNP9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="7">GSA</shortName>
            <ecNumber evidence="7">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="7">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hemL</name>
        <name evidence="9" type="ORF">AFR_01320</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357496.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01320"/>
    <dbReference type="EnsemblBacteria" id="AGZ38553">
        <property type="protein sequence ID" value="AGZ38553"/>
        <property type="gene ID" value="AFR_01320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.264"/>
    <dbReference type="eggNOG" id="COG0001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_11"/>
    <dbReference type="OrthoDB" id="493793at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ38553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="445" mass="45851" checksum="D283595E33D76CE7" modified="2014-01-22" version="1">MTTSYPAAGSPYPDEAPVSQALFDRAKDIVPGGVNSPVRAFKSVGGTPRFMVRGEGPWMVDADGRRYVDLVCSWGPLIHGHAHPAIVAAVQRAAALGTSFGTPTEGEVELAEEIVRRTPVEQVRLVSSGTEATMTAIRLARGFTGRSKIVKFAGCYHGHVDALLAAAGSGVATLGLPDSPGVTGAAASETIVLPYNDISAVEAVFRENDDIAAVITEAAAGNMGVIAPREGFNQRLAEITRANGALLVVDEVMTGFRVSPGGWAGLEPVDADLFTYGKVMGGGLPAAAFGGRTEIMSRLAPAGPVYQAGTLSGNPLACAAGLTSLRLADDSVYKRLDELSATVGALTSDALNTAGVPHRLSYAGNMFSIFFTDVDVVDYDSAKTQNAGAFKAFFHAMLSRGVYLPPSAFESWFVSAALDDAALEQIGAALPAAARAAAAAGGASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNU3</accession>
    <name>U5VNU3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MerR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357407.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00875"/>
    <dbReference type="EnsemblBacteria" id="AGZ38464">
        <property type="protein sequence ID" value="AGZ38464"/>
        <property type="gene ID" value="AFR_00875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.174"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045945_3_2_11"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="37506" checksum="009F72C9A8C3D4C1" modified="2014-01-22" version="1">MADEALTAGAVARRLGVAVTTLRTWHQRYGLGPSHHVPGQHRRYTAEDLARLQVMRRLTGQGVAPAEAAAWAQRIPIPIDLAKPLPAAPVVLVQDPEPPLAPSEDLPSLVDQDDAVLDGTYDTWPERLFTARDGGGHNLRTGRAGPAARGLARAAMRLDAPALRDILEMIVTDRGVVAAWEDVIMPVLIGIGERYAATKRFIEVEHLISRGITEVLGTVSRPTGLESPRVLLAAADEEQHTLPLEALAAALAQAGVPSRLLGARMPPEALRDAITRTGPTAVVLWSQLPSTGDPDQWAIVLSGPHRPLLAAAAGPGWSMDDLPEGITPLGSLTEAVRLVALAVTPHPRRSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQI9</accession>
    <name>U5VQI9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpsK</name>
        <name evidence="10" type="ORF">AFR_04645</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_015619020.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04645"/>
    <dbReference type="EnsemblBacteria" id="AGZ39218">
        <property type="protein sequence ID" value="AGZ39218"/>
        <property type="gene ID" value="AFR_04645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.938"/>
    <dbReference type="eggNOG" id="COG0100">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_5_0_11"/>
    <dbReference type="OrthoDB" id="1713391at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019981">
        <property type="entry name" value="Ribosomal_S11_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03632">
        <property type="entry name" value="uS11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="135" mass="14430" checksum="2821D4437686EA8A" modified="2014-01-22" version="1">MPPKARAGAPVKKVRRKERKNVAHGQAHIKSTFNNTIVSITDPTGAVISWASSGQVGFKGSRKSTPFAAQLAAEAAARRAMEHGMRKVDVFVKGPGSGRETAIRSLQAAGLEVGQISDVTPQPHNGCRPPKRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQR4</accession>
    <name>U5VQR4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">D-alanyl-D-alanine carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_04240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358082.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04240"/>
    <dbReference type="MEROPS" id="S12.003"/>
    <dbReference type="EnsemblBacteria" id="AGZ39137">
        <property type="protein sequence ID" value="AGZ39137"/>
        <property type="gene ID" value="AFR_04240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.856"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_2_3_11"/>
    <dbReference type="OrthoDB" id="537440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665641" evidence="1">
        <location>
            <begin position="26"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase" evidence="2">
        <location>
            <begin position="31"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="365" mass="39300" checksum="FD068C58CBDCEA0A" modified="2014-01-22" version="1">MSLRHLVAAAVVTACVIGTAQPVSAAPRDPVRQELRALVSEHGFPGALAAVRERDGDTRDLTAGPNVPVNGQVRIGSNTKTFVSVVVLQLVAEKKVELDAPVEKYLPGLVRGEGIDGRKITVRQLLQHTSGLPNYTEYLDTDGSFALQHTYWEPRDLLDLALAHPAVFAPGAKWEYSNTNYVVAGLLVQKVTGRPIAEQITDRVIRKAGLRHTYFPGVGDQSIRERHPHGYHPDESGELVDVTVLDPSWGWAAGQMIGTPEDLNSFFRALVGGRLLPPAQLKEMRTTVEAPEMWPGARYGLGIMSFPLSCGGVAWGHGGDIPGYETRGGVTEDGRGVSVAVTAMPSEEVAHNAVLKTVDTALCSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQZ8</accession>
    <name>U5VQZ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_04925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358328.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04925"/>
    <dbReference type="EnsemblBacteria" id="AGZ39274">
        <property type="protein sequence ID" value="AGZ39274"/>
        <property type="gene ID" value="AFR_04925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.995"/>
    <dbReference type="eggNOG" id="COG2019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1280947_0_0_11"/>
    <dbReference type="OrthoDB" id="1485018at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="23297" checksum="EBFFB2B09A34309F" modified="2014-01-22" version="1">MIVVVEGPTAAGKTSWCRRYVGRYVAEYAATGREPADEVALARHWARVSMRRWTEALDLERADGVAFCDTDPLKLHYAWGMAALGLAPRARFERELTVTREAFRVGRLGFADAILVGLPEVETLQRRRDTDETRRRRNFAAHVQLREPLQQWYNALDRLDPGRVSWELPAEGVPKLPVPRTDRCAVELLDALVAALPPLRQAVTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR43</accession>
    <name>U5VR43_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DeoR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_05150</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358842.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05150"/>
    <dbReference type="EnsemblBacteria" id="AGZ39319">
        <property type="protein sequence ID" value="AGZ39319"/>
        <property type="gene ID" value="AFR_05150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1042"/>
    <dbReference type="eggNOG" id="COG1349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060699_3_0_11"/>
    <dbReference type="OrthoDB" id="1629121at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014036">
        <property type="entry name" value="DeoR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018356">
        <property type="entry name" value="Tscrpt_reg_HTH_DeoR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00455">
        <property type="entry name" value="DeoRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08220">
        <property type="entry name" value="HTH_DeoR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00037">
        <property type="entry name" value="HTHLACR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00420">
        <property type="entry name" value="HTH_DEOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00894">
        <property type="entry name" value="HTH_DEOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="26908" checksum="5FEECCBCA2102560" modified="2014-01-22" version="1">MLAQQRQAAILDRVRAAGGVRVSELATEYGVSDMTIRRDLESLADRGLLAKVHGGATTVSPGSAHEPGFAAKSVRQRAEKAAMAMRAAALVSPGDAIALSAGTTTAGLAQRLVDVPSLTVVTNSIPVADIFYRAGRPDQTVVLTGGTRTPSDALVGPVAVAAIGTLHLDVLFLGVHGMSERAGYTTPNLMEADVNRALVEAAERLVVLADHTKWGTVGISSIVPLERADVVITDDQLDETARAVLAEQVNELMVVTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRC9</accession>
    <name>U5VRC9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase class B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05600"/>
    <dbReference type="EnsemblBacteria" id="AGZ39409">
        <property type="protein sequence ID" value="AGZ39409"/>
        <property type="gene ID" value="AFR_05600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1136"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056519_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="57"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="30122" checksum="C3777B7CEEADFCD2" modified="2014-01-22" version="1">MNETKVGDVTVTRIVETHGPIMPTGAFFPTMPQEAWDQHQDQLIPEHLDADGLVLAAMQTWLVRSEGRTILIDTAVGNNKDRPAVEGWNHQNVDYLGRLAEAGVRPEDVDLVVNTHLHVDHIGWNTRLADGEWVPTFPNATYLMPAVDFHFWNPANNPAVEGGVNQNAFADSIAPVHAAGQVQLWEDTHRIDGGLLLEAAPGHTPGHAVVKLESGGDRALFAGDIVHTPLQLEFRDHSSCFCEDPVQAGHTRRRLLSWAEDVNALLLPAHLDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRK6</accession>
    <name>U5VRK6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Beta sliding clamp</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_00010</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a ring-shaped head-to-tail homodimer around DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the beta sliding clamp family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357235.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00010"/>
    <dbReference type="EnsemblBacteria" id="AGZ38291">
        <property type="protein sequence ID" value="AGZ38291"/>
        <property type="gene ID" value="AFR_00010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2"/>
    <dbReference type="eggNOG" id="COG0592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038149_1_1_11"/>
    <dbReference type="OrthoDB" id="1040142at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00140">
        <property type="entry name" value="beta_clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001001">
        <property type="entry name" value="DNA_polIII_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022635">
        <property type="entry name" value="DNA_polIII_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022637">
        <property type="entry name" value="DNA_polIII_beta_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022634">
        <property type="entry name" value="DNA_polIII_beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30478">
        <property type="entry name" value="PTHR30478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00712">
        <property type="entry name" value="DNA_pol3_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02767">
        <property type="entry name" value="DNA_pol3_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02768">
        <property type="entry name" value="DNA_pol3_beta_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000804">
        <property type="entry name" value="DNA_pol_III_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00480">
        <property type="entry name" value="POL3Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00663">
        <property type="entry name" value="dnan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="5 9 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_beta" evidence="10">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_2" evidence="11">
        <location>
            <begin position="127"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_3" evidence="12">
        <location>
            <begin position="250"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ38291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="377" mass="39623" checksum="E4063C6C247A5746" modified="2014-01-22" version="1">MKFRVERDALADAVAWTAKSLPSRPSVPVLAGVMLRVTDGRLHVSGFDYEVSSQVSVEVQADADGAALVSGRLLAEITKALPNKPADIAAVGSHLELVCGSARFTLPTMPVEDYPTLPDMPSSAGTVSAQTFAAAVAQVAIAAGRDETLPMMTGVRIELNGTSMAMLATDRYRLAMRELEWSPDDPDISLNALVPAKTLNDTAKTMGPLGGEVTLALAQGAAGEGMIGFAGGTRRTTSRLLDGANYPPVRSLFPAAHNAEARVSVSSLVEVVRRVALVAERTTPVLLSFGEDGLVVEAGGTEEARASEAMEATFTGEPLTIGFNPQYLIDGLQNLGAQTAVLSFVDAFKPAVISPAAEDGEIVPGYRYLIMPIRVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRR7</accession>
    <name>U5VRR7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06115"/>
    <dbReference type="EnsemblBacteria" id="AGZ39512">
        <property type="protein sequence ID" value="AGZ39512"/>
        <property type="gene ID" value="AFR_06115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1242"/>
    <dbReference type="HOGENOM" id="CLU_1559647_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="17329" checksum="A0CB630DD8AA879C" modified="2014-01-22" version="1">MWDWDAEQRPPPAVLPGARAVLGAVLLLGLLAAGAGFLLRPSGSARPPSTDGQTLLRAREPVTQDLTIGADLLLTGGGGPARVRLDGVEVGTGRCAPVSTATVVVDVTVSGAAPVVPSAFTLTTAEGERVPGSAFCSAPSASVRIGFAATSVRLLGYTPYGGEPLGSWRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRR8</accession>
    <name>U5VRR8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_00335</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357300.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00335"/>
    <dbReference type="EnsemblBacteria" id="AGZ38356">
        <property type="protein sequence ID" value="AGZ38356"/>
        <property type="gene ID" value="AFR_00335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.69"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_0_11"/>
    <dbReference type="OrthoDB" id="1155802at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="CNNM transmembrane" evidence="10">
        <location>
            <begin position="4"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="224"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="289"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ38356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="444" mass="47473" checksum="746183708D2C634C" modified="2014-01-22" version="1">MDGLFLSAVLPLAAFALLTAGNAFFVAAEFGLVTVDRVEIAKQAEAGDRRARTVQNALHELSFQLSGTQLGITLTALLTGYLAEPALSRLFRPAIEPLFGGATETVTHVLSLVFATLVSMLFGELVPKNAALARPMRVALATAAPLRTFSKLFKWLISALNGSANWLVRRLGIEPQEELASARSPEELGLLAAISARAGALPTETATLLRRTIRFGEKRAAKAMTPRVDVVGLKTTASVADLIATSRQTGHTRFPVYESTLDLVVGVVGISDALGVPPERRAATPVSSVAREPVLVPESLDLDKVLAALRAADADLAIVVDEYGGTDGVVTVEDLIEELVGEIADEYDLDFTEAGSVELTAPDGDKTYLVDGLLREDEVAEQTGFRLPEGPYETLAGFLLSRLGHIPVAGESLEDDGWEFTVMEVDRHRIEQVRVVAPPEPADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS01</accession>
    <name>U5VS01_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MDMPI_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359289.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07345"/>
    <dbReference type="EnsemblBacteria" id="AGZ39758">
        <property type="protein sequence ID" value="AGZ39758"/>
        <property type="gene ID" value="AFR_07345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1499"/>
    <dbReference type="eggNOG" id="ENOG5032B92">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051661_2_0_11"/>
    <dbReference type="OrthoDB" id="2055578at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017517">
        <property type="entry name" value="CHP03083_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017520">
        <property type="entry name" value="CHP03086"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024344">
        <property type="entry name" value="MDMPI_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11716">
        <property type="entry name" value="MDMPI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03083">
        <property type="entry name" value="TIGR03083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03086">
        <property type="entry name" value="TIGR03086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MDMPI_N" evidence="2">
        <location>
            <begin position="14"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="20956" checksum="4B6F32941B6ABF05" modified="2014-01-22" version="1">MADRVPLDFAPPVRQVSSLLLGVTDEHLSRPTPCPDWKVGDLLDHLIGLGWAFTQTARKADPPEADGPPPEPSAANLSPDWRSRLPMVLEELVLAWQDPAAWEGTAKAGGVTMPAAAMGLVAVNEVTMHGWDLARATGQEYATDPRILEQLIEFLSQGPKEGSPGLFGPVVEIGDEADLLDQAVSLAGRDPNWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSA3</accession>
    <name>U5VSA3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359218.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06990"/>
    <dbReference type="EnsemblBacteria" id="AGZ39687">
        <property type="protein sequence ID" value="AGZ39687"/>
        <property type="gene ID" value="AFR_06990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1426"/>
    <dbReference type="eggNOG" id="ENOG5031K7Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076067_0_0_11"/>
    <dbReference type="OrthoDB" id="1840332at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665626" evidence="2">
        <location>
            <begin position="30"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="28124" checksum="27C96C79FF780F22" modified="2014-01-22" version="1">MNVSQVRPAVLAALFTVSVAVTGGCGAAADEVVAKPTPLGPRDALLKSLPDFTTGSFRFAVTGEVPASGEFDAGKHSYRIGFRYKQPDAGFTLHSDYLVVEKQTWIKIKFTGTEGLTGLPKLPKRWMLIDPAKVKDKDNVPLGYADETDPGDAGVVLRAVVDAEQTTEGKFTGTTDLTQQGKTEIVAADRLTALGDKAKQVPFEAATDSKGRLTRLVVKIPAAGKHKAGKYEVTYTDFGSAPTPVPPAADQQQKATAGAYELLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSF2</accession>
    <name>U5VSF2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359325.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07525"/>
    <dbReference type="EnsemblBacteria" id="AGZ39794">
        <property type="protein sequence ID" value="AGZ39794"/>
        <property type="gene ID" value="AFR_07525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1536"/>
    <dbReference type="eggNOG" id="COG3554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114504_0_0_11"/>
    <dbReference type="OrthoDB" id="1994921at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="177" mass="19246" checksum="1F7B0AA6665657B9" modified="2014-01-22" version="1">MTDLLEQLHADAPAPDVVPDLYGGLVGAWDVTNHYEDERSGVWRRGTVVWTFGRVLAGRAVQDVMWFTDDAGERSTGSTMRLFDPVSGAWRVVWFSPTGRTTALIGRPGESGDIVQEGERTGGQLVRWTFTAVTAESFRWLGHVSDDGGASWRLEQEMLARRREPAISGGGPGAAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSG2</accession>
    <name>U5VSG2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359335.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07575"/>
    <dbReference type="EnsemblBacteria" id="AGZ39804">
        <property type="protein sequence ID" value="AGZ39804"/>
        <property type="gene ID" value="AFR_07575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1546"/>
    <dbReference type="eggNOG" id="ENOG502ZFP0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1228762_0_0_11"/>
    <dbReference type="OrthoDB" id="1663019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="222" mass="22888" checksum="F4B83DD2E308334C" modified="2014-01-22" version="1">MSERPGNLPQTEAAPVEIAPEPFALTEPAEEETPEPRPQSRTKIIILGSLLAVGLAGASVIGYAGWRIASQKDATLTIPATVAGLSIDTSEEGRTTGEYLQTALSADVALDKAVGAVLTDGSDKDVLFFGGTTLIWTPEADLETAFGLVSDDQGSVTGLHDVPAGPLGGTMKCGTTKSDGADMPVCGWADHGSLALGMFPGRSESDAATLFKTIRSAAQTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VST8</accession>
    <name>U5VST8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02480"/>
    <dbReference type="EnsemblBacteria" id="AGZ38785">
        <property type="protein sequence ID" value="AGZ38785"/>
        <property type="gene ID" value="AFR_02480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.501"/>
    <dbReference type="eggNOG" id="ENOG5032WU7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051323_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="25706" checksum="BAD391ED09DAED4C" modified="2014-01-22" version="1">MTVMIDQDLAVFVPALPDPGTFGTAVPPLLDLVGRSLLDDADKAAVDAVTGASGSVALWRVWRLTPDSPPVRLYVLETAAAAPDGLDALVYRTGDEVPAELRRARNSGALLWTAADPAPLRVAPVFDSNAGFDPAHVRLDDPDRDRIADRLDGGSPILATADRLTDVVDPARGAVVPMSYRTDGRWIWTESVSYYLRTYGLAPGTEFLAHLRAAGRPATDAAGEHRALAALFQSAAAVRVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSW3</accession>
    <name>U5VSW3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357679.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02235"/>
    <dbReference type="EnsemblBacteria" id="AGZ38736">
        <property type="protein sequence ID" value="AGZ38736"/>
        <property type="gene ID" value="AFR_02235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.452"/>
    <dbReference type="eggNOG" id="ENOG5031RMA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094182_0_0_11"/>
    <dbReference type="OrthoDB" id="1524864at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025324">
        <property type="entry name" value="DUF4230"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14014">
        <property type="entry name" value="DUF4230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26930" checksum="58C8724D23A7D9F8" modified="2014-01-22" version="1">MAQSSETDEPTREYPEVEDQPTPAPAEPARRMGGFARVLVFTGVVFALIVAMCLGLRAVNVLPSFDNPFSDKTTDRSQPVLLQSMRDLSRYVAADGTFQVIVDLQENKENIPDFLLNKRTLFVGSGTVEEYVDFSTLAEGAITVNNDDKSVKIELPAPQQGQAALDMERSYVVAEERGLLNRIGGAFSSNPDQQQRVYQMAQQRISEAARSSGLDQRARDNTQKMLQGLLTSLGYTSVTVTFASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSY5</accession>
    <name>U5VSY5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_08190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359458.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08190"/>
    <dbReference type="EnsemblBacteria" id="AGZ39927">
        <property type="protein sequence ID" value="AGZ39927"/>
        <property type="gene ID" value="AFR_08190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1671"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_3_1_11"/>
    <dbReference type="OrthoDB" id="1891092at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="9">
        <location>
            <begin position="22"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="28953" checksum="DBAF5C40FF052AAF" modified="2014-01-22" version="1">MLTLVLPRLVSFEGSARRSTSMVERNVATLRSAYWLVMASGFLEPVLYLFSIGVGVGALVGDLTLPDGRVIDYAAFVAPAMLASSAMTGALSETTFNFFGKMKFMKLYDGILATPVRPIEIAAGELAWAMIRGNIYAAAFLVIMVVMDLTTPGRALGAFGAAILVGFAFGGVGMALSTFMRSWQDFDLMSSAQFALFLFSGTFVPAQAYPAVLRWVVEVTPLYRSVDLIRAVTTGSVGWIQLVDVVYLLALLALGLAVAGRRMEKLLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT57</accession>
    <name>U5VT57_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Putative M50-family peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AFR_09295</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359742.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09295"/>
    <dbReference type="EnsemblBacteria" id="AGZ40148">
        <property type="protein sequence ID" value="AGZ40148"/>
        <property type="gene ID" value="AFR_09295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1893"/>
    <dbReference type="eggNOG" id="COG1994">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066211_0_0_11"/>
    <dbReference type="OrthoDB" id="1401629at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06158">
        <property type="entry name" value="S2P-M50_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044537">
        <property type="entry name" value="S2P-M50-like"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="243"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ40148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="275" mass="29389" checksum="D3F38C31B7EEAC79" modified="2014-01-22" version="1">MSYQVSQGTPKSAFVPSPVFVGILAVTAVAGWMTWTGYGNVRVDVFLLVVSGWVLSLCLHEYAHAIVAYFSGDRGVADRGYLRLNPLKYTHPVLSIVLPLVAVILGGIGLPGGAVWVDHAHIRSKAKESLISAAGPATNVVFALLLAIPFAFGAGSDVVLMSDGSGGVVVGGNHGDFWVALGALAFLQVTASLLNLLPVPGLDGGNIVQPWLSPPYQRAYNLFAPYGFILLFVLLWQSRINEWFFTLVSWLAGLIGVPDGLGEVGLFYMRFWSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT93</accession>
    <name>U5VT93_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359831.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09520"/>
    <dbReference type="EnsemblBacteria" id="AGZ40193">
        <property type="protein sequence ID" value="AGZ40193"/>
        <property type="gene ID" value="AFR_09520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1941"/>
    <dbReference type="eggNOG" id="ENOG502ZKTM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047832_0_0_11"/>
    <dbReference type="OrthoDB" id="1661570at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="440" mass="46793" checksum="939D3D77A1A34AE4" modified="2014-01-22" version="1">MSRVLGIELRRSAAAGAGLALLVVGVLLLFFAEGIDFSTGWVQLAMTQRLYLAVLWPLALAAGAFQGRRDHRSNVTELFSTTPRPRMHRTMPTLGAMAISVVCAYLVMGIAGGLWIITTAEYFPVTVFVVTAVGALALIAAAWFGLAIGRLLPSIITAPALGVAGLALLLLIPMATRPRGWLALVFSPIYEMNMPGAYATVPTRVSISQTLWLIGLAVAGVLLFASSGWRVRVAALLPLVAGAALAITVMPHQNRYVTDALDPVAQELVCTEDEPRVCVSRVHSALLGEVTPRAREALQILAKLPGAPTQAHEDTTYYPDSFPPRRTDTVLIPVEADDKGHLADPDTVVPRMLDAAGANGIGCEQPADLVVTRAAGSWISGREPVATYEYEDPSINPAAVQLWEKLRSLPEADAAARVSAVRQAVLACKDVKGLLDRSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTN2</accession>
    <name>U5VTN2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357995.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03805"/>
    <dbReference type="EnsemblBacteria" id="AGZ39050">
        <property type="protein sequence ID" value="AGZ39050"/>
        <property type="gene ID" value="AFR_03805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.768"/>
    <dbReference type="eggNOG" id="COG4886">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850853_0_0_11"/>
    <dbReference type="OrthoDB" id="2094096at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="15962" checksum="01CDFFCF76C9BF7C" modified="2014-01-22" version="1">MIIDYLMTGLAGYDPDEFPQVQGFEVRLEPGAHGFELTFTTTQPDHVVEWAWITERDHEIWAWTIGDVPCGTNAKPYRVSDQGWNILVWEQNGHVFVAQGEGEWDYERDADTFTTWFKVTSDLYRAAWTTALEELTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUA2</accession>
    <name>U5VUA2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome bc1 complex Rieske iron-sulfur subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytochrome bc1 reductase complex subunit QcrA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_10540</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Iron-sulfur subunit of the cytochrome bc1 complex, an essential component of the respiratory electron transport chain required for ATP synthesis. The bc1 complex catalyzes the oxidation of menaquinol and the reduction of cytochrome c in the respiratory chain. The bc1 complex operates through a Q-cycle mechanism that couples electron transfer to generation of the proton gradient that drives ATP synthesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10540"/>
    <dbReference type="EnsemblBacteria" id="AGZ40397">
        <property type="protein sequence ID" value="AGZ40397"/>
        <property type="gene ID" value="AFR_10540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2149"/>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_1_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="PTHR10134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="12">
        <location>
            <begin position="61"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ40397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="14249" checksum="FFCEB4F24D22884F" modified="2014-01-22" version="1">MLVGAGALGAGCLLAACGTDSTGTGSNGANGGDFSNDPAPAGSKGADAGGTSTSGGDSGGSALALVADIPEGGGVIKGDYVITQPASGTFKAFSKVCTHAGCDVSKVDAGVITCPCHGSTFSIEDGSPTGGPATKALPETKVKVDGDNIVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVB3</accession>
    <name>U5VVB3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative response regulator receiver domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359176.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06780"/>
    <dbReference type="EnsemblBacteria" id="AGZ39645">
        <property type="protein sequence ID" value="AGZ39645"/>
        <property type="gene ID" value="AFR_06780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1385"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_11"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="16022" checksum="E40F3E526DF3E7B4" modified="2014-01-22" version="1">MRSLEILVVDDDDADALMISEALENADRSTTVNRVADGREALDYLLRTPPYEQAQRPDLILLDLNMPRMDGRETLAAIKTDDRLKAIPVVILTTSGAAPDIESSYQHRANAYVTKPFGLDEFEATVRTIDRFYREVALLPPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVC0</accession>
    <name>U5VVC0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_13335</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361012.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13335"/>
    <dbReference type="EnsemblBacteria" id="AGZ40953">
        <property type="protein sequence ID" value="AGZ40953"/>
        <property type="gene ID" value="AFR_13335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2708"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_11"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="24"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="293"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ40953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="387" mass="40233" checksum="C4BFB33B6C06F8B1" modified="2014-01-22" version="1">MRLKPLDLLELGLIGVRTRRMRAALSVLGIAIGIATMVVVTGIPASSGAALQRRLAALGVDLLKVVPAQQDPPAVFPAESVAMIRRIGPVTAVSAVAGTSAVVRRSDRVDAADGSGLTVRATRADLLGVLHGRVRSGHWLTPAEQQFPAVVLGSVAATRLGFDAPGQQVIIDRTWFTVVGILDTIPLSPEIDRSVLVGWESARSVLGFDGRPTIIYVRVREDSVEAVREVVPPTVHPEQPHQVTVTRPSDALAAKRATESAFSVLMLGLAAVALVVGGVGVANTMVISVLERRSEIGLRRALGSSRGQIRAQFLTEAIVLSALGGATGTVSGLILTAAYAAHQDWPLVVPLPALLAGLGGSVTVGILAGVYPSVHASRLTPTEALAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVG6</accession>
    <name>U5VVG6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD linked oxidase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360923.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12890"/>
    <dbReference type="EnsemblBacteria" id="AGZ40864">
        <property type="protein sequence ID" value="AGZ40864"/>
        <property type="gene ID" value="AFR_12890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2618"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018354_10_0_11"/>
    <dbReference type="OrthoDB" id="330793at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006093">
        <property type="entry name" value="Oxy_OxRdtase_FAD_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00862">
        <property type="entry name" value="OX2_COVAL_FAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="2">
        <location>
            <begin position="44"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="453" mass="46597" checksum="39125CA46B886F19" modified="2014-01-22" version="1">MPSNPDLSPAAEAAAALEDVVRAERIVTDAAAIDAAVPPWNGAVADRPAIVVRCAEVGDVRAGISVARRLGLPLSVRGRGHDWAGRSLRAGGLTLDLSGLSSVRVDAEKGRAIVGGGAGIGDVLRATGDAGLVAVTGTTDSVGFVGLAIGGGYGPLSGRFGLAADNLVAATVVLADGSVVNADPDLLWALRGGGGNFGVVVEAQFDVHRVPSVVGGAVIYPFDQATQVLKGLRELHAEMPDELSVQTALLSGPDGAPVVLLSPTWCGPLAAGLAGDGPVQALARLGTPLMAQIGEKRLADGAAELGAMFPYGRHVEIRTRSVPVLSEDVAEALADHVARKPSPLTAVSLHPFQGAATRVAPQATAFGTRDPHHMIEIISVWTDEHESPVQQQWTRDLDAALKPYALPGGYPNLLAADAVDQIEFGFGGNAGRLRSLKAHFDPSEVFSAIPLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVJ2</accession>
    <name>U5VVJ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molecular chaperone GroES</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13015"/>
    <dbReference type="EnsemblBacteria" id="AGZ40889">
        <property type="protein sequence ID" value="AGZ40889"/>
        <property type="gene ID" value="AFR_13015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2643"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="18"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="328" mass="34106" checksum="64E200E40A3124BB" modified="2014-01-22" version="1">MIMSDNNMMNAVRQHAFGGPETLVVERVERPAPLPTEVLVRVHAAGINPVDWKTRGGTGMAGVLGEPPFILGWDVAGVVEEIGFGVTTLAPGDKVYGMPWFPRAAGAYAEYVTAPARQFACMPAAASFEEAAAVPLAGLTAWQALVDTIGVQPGQRVLITAAAGGVGHLAVQFAKHLGAYVIAVAGPRNQQWVAELGADEVIDYTACRFEKEVSDVDVVLELVGDAFDATSTRSIETLRPGGLLVAVPAGVSPELAAAGKAAGVRVSPFLVEPDAPALARIAALIDDGSVTVEVAKVLPLAEVADAHREIELGRTRGKIVLRVAGDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVP2</accession>
    <name>U5VVP2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359316.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07480"/>
    <dbReference type="EnsemblBacteria" id="AGZ39785">
        <property type="protein sequence ID" value="AGZ39785"/>
        <property type="gene ID" value="AFR_07480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1527"/>
    <dbReference type="eggNOG" id="COG4198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_679053_0_0_11"/>
    <dbReference type="OrthoDB" id="591335at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008323">
        <property type="entry name" value="UCP033563"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36454">
        <property type="entry name" value="PTHR36454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06245">
        <property type="entry name" value="DUF1015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="396" mass="41214" checksum="1A2A133EC44B39D3" modified="2014-01-22" version="1">MTVVHPISRAWITTGGTGAQNYDEFADDAEITAIIAANPHSALAIEMPHRAPDSVGKAFLDCLPDAAARLAEAKAAGGYSPASDVVVLYRITAPGEPAAYGLWSMVDTDQISTSADEPGLVIRNEDVFIAKVRERVALAEALQTLLSPVLLLQTGKGDELHAALAAATDAAGAPAATDTDQSGRTHAIWVIPAGPLQDELTALAGGGELVVADGNHRSLAAQTGGLPRFLAVVTTPASVAIQPYNRLISELPAADFVERLTAAGARVEELPRPAAVPAKGTIELYAGGRSFSVVLPSAEGAGAVDNLDHALVERILLGDVLGLDPGDKRISYVGGDYPAEWLRGEVDAGRAELAVLIAPVTVDDFVAVNLARQKLPRKSTWFTPKARGGLVLAQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVQ1</accession>
    <name>U5VVQ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361013.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13340"/>
    <dbReference type="EnsemblBacteria" id="AGZ40954">
        <property type="protein sequence ID" value="AGZ40954"/>
        <property type="gene ID" value="AFR_13340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2709"/>
    <dbReference type="eggNOG" id="COG3967">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_6_11"/>
    <dbReference type="OrthoDB" id="1261526at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="26202" checksum="268DE3CC15B6F2F1" modified="2014-01-22" version="1">MDIKNRTVFIAGGTSGIGLGLARRFAAAGGTVIVGGRRPGSVDGLSTVELDVTEADSVRRARDTVLESHPDLDLVITMSGVQLTEDLRDPGHFATAETTVQTNLLGTIRVLDAFTPHLLARGAGTFVTVSSGIGFLPFPLMATYAASKAGVHAYTEALRAQLAGTGVEVTELVPPAVATTPEHKRLNPRALDLDAYLDEVLELLGQDPTPDEILVQGVLQHRWAERDGTYADLVAQRSQALETLPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW46</accession>
    <name>U5VW46_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoribonuclease YbeY</fullName>
            <ecNumber evidence="7">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ybeY</name>
        <name evidence="8" type="ORF">AFR_07960</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359412.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07960"/>
    <dbReference type="EnsemblBacteria" id="AGZ39881">
        <property type="protein sequence ID" value="AGZ39881"/>
        <property type="gene ID" value="AFR_07960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1625"/>
    <dbReference type="eggNOG" id="COG0319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106710_3_2_11"/>
    <dbReference type="OrthoDB" id="1830156at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020549">
        <property type="entry name" value="YbeY_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986">
        <property type="entry name" value="PTHR46986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00043">
        <property type="entry name" value="TIGR00043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01306">
        <property type="entry name" value="UPF0054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <keyword evidence="6 7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="16679" checksum="C7A97CB3C46ABCC4" modified="2014-01-22" version="1">MSIEIANESGVEVDTDAILAVARHALEEMGVNPLAELSILLVDIDYMTELNHRWMGSDGPTDVLAFPMDEGSVDHGPGEAGTGEPALLGDIVLAPEVAAKQAAAAGHTPADELHLLTVHGVLHLLGYDHAEPEEEREMFALQARLLQSWRSGRRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW73</accession>
    <name>U5VW73_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359516.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08480"/>
    <dbReference type="EnsemblBacteria" id="AGZ39985">
        <property type="protein sequence ID" value="AGZ39985"/>
        <property type="gene ID" value="AFR_08480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1726"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_2_1_11"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="49"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="270" mass="29143" checksum="0BCBEBCDCD52085E" modified="2014-01-22" version="1">MTSSLGLDGIKARQQKTWASGDYGAVAALIHPMSESLVQAADLTAGSRVVDIATGTGNAAIAAARCLCDVTGVDYVPELLERGRARAQAEHLPVTFVEGDAEALPCRDGEYDAAFSVVGIMFAPDQEKSAAELTRVVRSGGTIGMANWAPDGFIGELFRTVGRRVPPPPGVRAPVEWGSEPRLRELFGDRVRDLRVTRRDFVFRFGSPDDFADYFRVHYGPTLKAFEALDDDNGKLLHADLVDLATRYNVATDGTAKIPSAYVEVLATRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWL4</accession>
    <name>U5VWL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361457.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41398">
        <property type="protein sequence ID" value="AGZ41398"/>
        <property type="gene ID" value="AFR_15580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3164"/>
    <dbReference type="HOGENOM" id="CLU_2784533_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="68" mass="7466" checksum="3CBD9346CA25B2EB" modified="2014-01-22" version="1">MQETMRAFREDAGYYAHGGATNVVSLKFTVAVLQELAPGMVLTGFGYQDQRVVSEAEFEETAQEDCDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX44</accession>
    <name>U5VX44_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Arginase/agmatinase/formiminoglutamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361503.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15810"/>
    <dbReference type="EnsemblBacteria" id="AGZ41444">
        <property type="protein sequence ID" value="AGZ41444"/>
        <property type="gene ID" value="AFR_15810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3209"/>
    <dbReference type="eggNOG" id="COG0010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_6_2_11"/>
    <dbReference type="OrthoDB" id="1598732at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782">
        <property type="entry name" value="PTHR43782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="30702" checksum="AC391A414153549A" modified="2014-01-22" version="1">MNGSDWFLLGAPWDCSGTGRGEARAPAALRDAGLADLVDLDLGDAATVITSTDRDRTTGVRALPETVAAAHALSAALWTGVRDHPGRRPLVLGGDCSLLLGVFAGARAAVGELGLWLLDGHPDYLDAPSSDTGETADLELAILLGDGPPELTRATPMLAARNAALLGHRTTSLDPASAAEVARLPDDLFTIDSPALLADPRGAGERAAAWAAVPMWLHLDVDVLDPSVLPAVTYPQGGGPDLDQLAAVLAPLLASPRLIGVSVADFRPDLDPDHRYAKRLVSLLSGQPKIEVTHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX83</accession>
    <name>U5VX83_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA polymerase IV</fullName>
            <shortName evidence="13">Pol IV</shortName>
            <ecNumber evidence="13">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">dinB</name>
        <name evidence="16" type="ORF">AFR_09910</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="13"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09910"/>
    <dbReference type="EnsemblBacteria" id="AGZ40271">
        <property type="protein sequence ID" value="AGZ40271"/>
        <property type="gene ID" value="AFR_09910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2021"/>
    <dbReference type="eggNOG" id="COG0389">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012348_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03586">
        <property type="entry name" value="PolY_Pol_IV_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01113">
        <property type="entry name" value="DNApol_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022880">
        <property type="entry name" value="DNApol_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024728">
        <property type="entry name" value="PolY_HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11798">
        <property type="entry name" value="IMS_HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="SSF100879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 13 16" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 13" id="KW-0515">Mutator protein</keyword>
    <keyword evidence="4 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UmuC" evidence="15">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Substrate discrimination" evidence="13">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AGZ40271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="43006" checksum="ABF7C9A0A92CF6D9" modified="2014-01-22" version="1">MDAFFAAVEVRRRPELRGKPVVVGGAGPRGVVSSASYEARRYGIRSAMPGMRARALCPHAIFLPPDFSEYTAASRAVMQIFRDVTPLVEPLSLDEAFLDVAGAQRLLGRPAVIARDIRARVAEEQRLTCSVGVAPSKFVAKLGSTRAKPDGVIVVPAGQVLDFLHPLPVDALWGVGEKAAETLHRLGLMTVGDLAAAPLGMLRGALGEASSAHLHELAWGRDPRRVVSEHQEKSVGAEMTYDIDVTDPAVIRRSLLALADKVGARLRAAGHVGRTVTIKIRMADFRTVNRSRKMPTATDVAREIFETSWALFKALAPGESIRLVGVRVEGLASAATTSRQLTLGEPERGWREAEAAADAVSARFGRSIVGPASLLGQTDLRRTENHPHSTVVPLSDPPTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXE0</accession>
    <name>U5VXE0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360094.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10185"/>
    <dbReference type="EnsemblBacteria" id="AGZ40326">
        <property type="protein sequence ID" value="AGZ40326"/>
        <property type="gene ID" value="AFR_10185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2075"/>
    <dbReference type="eggNOG" id="COG3324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141550_0_0_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="113" mass="11770" checksum="03A9FC90834F3F04" modified="2014-01-22" version="1">MATSGLGTIVIPVKDLTAAKTLYGTLLGVTPDVDQPYYVGFTAGGQHVGLDPNGDTTAGPIMYWHVDNIRTTLDALLAAGAEQLQEIKDVGAGKKIATVRDQDGTTVGILEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXL1</accession>
    <name>U5VXL1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PMT_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_16510</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16510"/>
    <dbReference type="EnsemblBacteria" id="AGZ41584">
        <property type="protein sequence ID" value="AGZ41584"/>
        <property type="gene ID" value="AFR_16510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3348"/>
    <dbReference type="eggNOG" id="COG5305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024191_0_0_11"/>
    <dbReference type="OrthoDB" id="1664452at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="PMT_2" evidence="9">
        <location>
            <begin position="85"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ41584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="507" mass="54312" checksum="BDE090510CCDF348" modified="2014-01-22" version="1">MLSRPVPPVVAQQAEPPPRRWSLLSTVLLALPPAALALTLSVLGIGSRSMWNDEYATWYASTLSFRDLSKLLVNVDAVVAPYYVVMHYWIVLFGDSETSLRLPSALAMAGTAALTALLGRRLFDTGTGLAAGLILGGLPSVTRYGQEARPYAFAIGFAVLATLLLLRAMERPDWRRWLLYAAGLILAGLVHIVTLTVLLAHAAFMARAFRVSGDLRLLRWIAGASLAVTAALPLAAKGSDQASVIAWIKADGAAVTGLPERIFGSWQVALVVTAAALLATVLLGARHRGSLVLLLSWAIFPPVFCYVTFPLFHLFLHRYLLFTLPAWALLAAAVGYSLVRFTGRLSSKVPVSLSALLVLAAMLLVSGPGQRAARHSPVYGEPDFRGAAAAVLAAARPGDGIAYAGSTRNGRRAFDYEARDAAMPRDVLVHRTSQANGTFGAEECADPGLCVGDTGRIWLVSTTQSNLSPMDGMPGATQGFLLTAYTVVPYRTFENVRIFALDRKDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY85</accession>
    <name>U5VY85_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative cellulose/chitin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_17880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362181.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="SMR" id="U5VY85"/>
    <dbReference type="STRING" id="1246995.AFR_17880"/>
    <dbReference type="EnsemblBacteria" id="AGZ41854">
        <property type="protein sequence ID" value="AGZ41854"/>
        <property type="gene ID" value="AFR_17880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3627"/>
    <dbReference type="eggNOG" id="COG3397">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063855_0_0_11"/>
    <dbReference type="OrthoDB" id="1005693at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03067">
        <property type="entry name" value="LPMO_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665480" evidence="2">
        <location>
            <begin position="35"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="3">
        <location>
            <begin position="269"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="38320" checksum="8F888FADFCC8082A" modified="2014-01-22" version="1">MSSSLRIRILVSGLVGVVVATLAAVVIPAGPASAHGNVTGPASRNYGCYERWGSKFQDPAMATEDPMCWQAWQANPSAMWNWNGLFREGVAGNHQAAIPDGQLCSAGRTESGRYNAMDTVGDWKAASVGNNFNVRLFDGALHGADYIRVYVTKPGFNPVTTALKWSDLNLVSQIGNTPAAQWQTANGGVQIDVPVALSGRTGRAMVYTIWQASHLDQSYYFCSDVNFSGGSTTPPVTTPPATTPPVTTPPVTTPPVTTPPVTTPPVTTPPPATGACSATYAVGSTWSGGFQGDVTVKAGTSAIKGWTVKLTYPGSQTVQQGWNATVSASGTTVTAANASYNGALSAGATTNFGFIGSGTSSTPAVTCTATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ43</accession>
    <name>U5VZ43_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_19880</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362620.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19880"/>
    <dbReference type="EnsemblBacteria" id="AGZ42248">
        <property type="protein sequence ID" value="AGZ42248"/>
        <property type="gene ID" value="AFR_19880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4034"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_11"/>
    <dbReference type="OrthoDB" id="1108236at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="10"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="514" mass="52073" checksum="0AE5C9B5E5D79693" modified="2014-01-22" version="1">MVTSKSRWWALAALALCTLAIGLDSTVLSVALPTLAGDLHASTGDLQWITTSYLLVLAAALLPAGMLGDRFGRKRMLLGALVVFGAASVACAYAVSTGQLIAARAALGLGAAVLMPLTSAVLTVLFDADERPKALTVWVTASALGIPLGPVVGGWLLDHFWWGSVFLINIPMVLLGLVAVALWVPESHGDRARRFDPAGIVLSTAGLVALTYGIVEAGERGWTDPRSLITAIGGAVLLAVFGWWQRHAQAPLVELALFRSRGFTWGSLLATVGSFGLFGILFALPQFFQSIGGHDALGTGLRLLPVIGGLLVGARVAGRLEPRLGAKVVVTAGLVLMAAGLFAGAATGPATGYAYVALWISVAGLGLGFTMPPAMNAALGALTPERSGVGSGLIQALRQVGGAIGVTVLGTVLNGAYRDQVDVTGLPPAAVDAVRDSATAGVAVAGNLGDQALLQSVQAAFTSGMDLALVVAGAVAVAGAVLSLLFLPARSPAPEADIVTPSPAGPLAAAESVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZB4</accession>
    <name>U5VZB4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_19125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362470.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19125"/>
    <dbReference type="EnsemblBacteria" id="AGZ42097">
        <property type="protein sequence ID" value="AGZ42097"/>
        <property type="gene ID" value="AFR_19125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3884"/>
    <dbReference type="eggNOG" id="COG2518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067079_1_0_11"/>
    <dbReference type="OrthoDB" id="1337662at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006764">
        <property type="entry name" value="SAM_dep_MeTrfase_SAV2177_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04672">
        <property type="entry name" value="Methyltransf_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017393">
        <property type="entry name" value="MTase_SAV2177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28433" checksum="1AE2B7F8C81EB3B2" modified="2014-01-22" version="1">MGDADADFSSRPSSARIYDALLGGNHNFEADREAAKRLLSMIPAAGEMARANRAFLNRAVQFLLDAGVRQFLDIGSGIPTVGNVHEIAQRRVPEARVVYIDIDPVAVAHAGEILADNPRATVVQADMRFPEAILEHPGVRRLIDLDQPVGLLLVAMLHFVPEDDAFVAVDRLREALPAGSYIVISHGVSETAADSELEGITALYRRTDVSAASGRTRAEIMRFFGDTELVPPGLVWVHEWPDGPQDKTGPADMAVVAAIGRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZP4</accession>
    <name>U5VZP4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carboxymuconolactone decarboxylase domain /Cupin 2 barrel domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14515"/>
    <dbReference type="EnsemblBacteria" id="AGZ41185">
        <property type="protein sequence ID" value="AGZ41185"/>
        <property type="gene ID" value="AFR_14515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2948"/>
    <dbReference type="eggNOG" id="COG0599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042241_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="2">
        <location>
            <begin position="40"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="134"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="253"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="37586" checksum="14234FC695517AC0" modified="2014-01-22" version="1">MITRVQDQTSAAGPSDTFTGTVRVDTLFPADATAPYSGAYVTFQPGARSAWHTHPAGQRLVVTAGVGRTQQWDGPVQEIRAGDVVWCPPGVKHWHGAAPDSVMTHLALTGVLDEQAVTWLEHVSDDQYGDHRQTGALSEQHAALALIGASTAGGDLPRLTTALTQALDAGVTVNEVKEALVQMYAYAGFPRSLNGLGTFMTVLKQREDRGIKDETGPEPSPLPDRTDILALGTRTQTELTGAPVTGALFDFAPAIDEFLKTHLFGDIFGRDNLDRRSREVVTIAALATLTGTESQLRSHFAIGLNTGLSEAQLRGLLQVLGQEAGNAARILDEVLQDGQKEMPCPPYAQPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZT0</accession>
    <name>U5VZT0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362649.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20025"/>
    <dbReference type="EnsemblBacteria" id="AGZ42277">
        <property type="protein sequence ID" value="AGZ42277"/>
        <property type="gene ID" value="AFR_20025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4063"/>
    <dbReference type="eggNOG" id="ENOG5033KTX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091243_0_0_11"/>
    <dbReference type="OrthoDB" id="1738608at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="23875" checksum="8637698B50E6E001" modified="2014-01-22" version="1">MTDHGDQLRRAFETHENEAPDPAQVYARVQELAKGYQWRRRGAQVAGGAVLSAGLIAGVLSVPSLFPGSAPNDSVATAPNMQVGAAPAASPSVKPSALPKESEEPVNIVDAHQAEYDAFFGAGYEFADAEKLAKIWKMPKSDIDAVKIAAGKKLIQGKKLPVKPNPANVVDAKKEKQVNAFFEAGYDVEDAVKLSKIWKLDTAYDAKVAGGKRLLAGETLPLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W082</accession>
    <name>U5W082_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Virginiamycin B lyase</fullName>
            <ecNumber evidence="1 2">4.2.99.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 2">Streptogramin B lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">vgb</name>
        <name evidence="3" type="ORF">AFR_21715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Inactivates the type B streptogramin antibiotics by linearizing the lactone ring at the ester linkage, generating a free phenylglycine carboxylate and converting the threonyl moiety into 2-amino-butenoic acid.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Vgb family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.-" evidence="1 2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362985.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21715"/>
    <dbReference type="EnsemblBacteria" id="AGZ42613">
        <property type="protein sequence ID" value="AGZ42613"/>
        <property type="gene ID" value="AFR_21715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4400"/>
    <dbReference type="eggNOG" id="COG4257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054751_1_0_11"/>
    <dbReference type="OrthoDB" id="966287at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016835">
        <property type="term" value="F:carbon-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017001">
        <property type="term" value="P:antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01282">
        <property type="entry name" value="VirginiamycinB_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011217">
        <property type="entry name" value="Streptogrm_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026412">
        <property type="entry name" value="Streptogrm_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR026412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30686" checksum="D09E0AEEABCE7949" modified="2014-01-22" version="1">MNIREITVAGAQAGPYGITAGPDGALWLTFTHAGRIARLTTGGEVTTFDLGAPDSQPMVITSGPDGALWFTRNRDDRIGRITTGGDLTDFALPTPGSGPYAITTGPDGALWFTEMNTDRIGRLTPEGDLREFEVPAEKAFVSVITSGPDDALWFTLNQADAVGRITTDGQVTLHQLPTQGAAPVGITATADALWFVEIGAGQVGRITPAGRIDEFALPDTAARPHAITPDATGGCWLTEWGANRVAHITATGEITEHELPTPKSEPHGLVLGPDGNLWVALETGGVAVLECAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0A2</accession>
    <name>U5W0A2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_20950</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362834.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20950"/>
    <dbReference type="EnsemblBacteria" id="AGZ42462">
        <property type="protein sequence ID" value="AGZ42462"/>
        <property type="gene ID" value="AFR_20950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4247"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_11"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="36080" checksum="D609D2E7E55632E4" modified="2014-01-22" version="1">MNGVAVHLDGLRRSFGGVHALDGLSLEMQPGELIALLGPSGCGKTTALRILAGLEDADSGRILVGGKDISGLPANRRDMGMVFQAYSLFPHLTALENVQFGLKLRRRASGDRAAKMLDLVGISAHAARYPHQLSGGQQQRVALARALAIEPQVLLLDEPLSALDAKVRVQLRDEIRRIQTEVGTTTLFVTHDQEEALAVADRVGVMRSGRLEQLAEPSEVYLRPASAFVADFVGLSNRLPGRLDGDAVSVLGTRLPLVQPASGGPEVTALVRPESVDVVPDTDGDARVVSTTFLGPTSRVTVEVGESLVVAQVTGERLADLAVDTPVRLVLRPVPVALEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0E1</accession>
    <name>U5W0E1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative asparagine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_21150</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparagine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362874.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21150"/>
    <dbReference type="EnsemblBacteria" id="AGZ42502">
        <property type="protein sequence ID" value="AGZ42502"/>
        <property type="gene ID" value="AFR_21150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4287"/>
    <dbReference type="eggNOG" id="COG0367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_3_0_11"/>
    <dbReference type="OrthoDB" id="610569at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666256" evidence="7">
        <location>
            <begin position="23"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="371"/>
            <end position="372"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="6">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="639" mass="70128" checksum="11DAA994DC88043B" modified="2014-01-22" version="1">MCGLSVFVSSRPHAGVPDPASAAAFAAALDTMHHRGPDDTQVEFGDGLAFGFKRLAIVDVAESRQPAHYPADGPQAGRWTVVFNGEIYNYRELRADLIREHQATFATQGDTEVLAAAFHYWGAAALPRLRGMFAFVAYDHWTGTVHAVRDPFGIKPLYLLETADGLFIASERKALLPFSGDSGAALDTDALAHYLTLQYVPDPLTLHRHVRRLPPGHRMTWTPGGGQRLHRYFRPSLRPLNVRPDAAIHAIRDSLRDSVRKHLHAEVPVGAFLSSGVDSSAIVALAAEVKPDLHAFTAGFAHEGYSEIEIAQDTANQLGVRLTPTVVTEDDVIRELPRIVQLLDDPVADPSLVPLYFLAETASRYVTVVLSGEGSDELFGGYTIYREPMSLAGIERLPAGMKRGLRNLSKVIPEGVKGHSFLERGTTPIEERYYGNARIFSPAEKAELMRFTADPHTTITASLYAETADVDDVASMQYVDLHTWLPGDILAKADRMSMAHSLELRVPFLDQAVYAAAAGLPTELKLPAGNRTTKWALREAMRGIVPESVRDRRKLGFPTPTRLWLKGDIGDWIADLMDRSPTGHLLDLGYAQRLLADHRAGVADNSRKVWTVAMFCLWHAITVEKAIDPSPARPAVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0E4</accession>
    <name>U5W0E4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361460.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41401">
        <property type="protein sequence ID" value="AGZ41401"/>
        <property type="gene ID" value="AFR_15595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3167"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_718924_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665409" evidence="1">
        <location>
            <begin position="24"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="384" mass="40719" checksum="ECEF235BE4BD0E70" modified="2014-01-22" version="1">MHRIVVSALACLTLAAAAVPAGAAPVTGPDASGASRLRMAWSVTPKMGVGTAPVYLAGSTYHLEGVRDPTNARSLLRVVRHDAGTGRALSFATGAIESIMPYKPVTSGSTLFVTTYREDTQIRAFRPDGRLLSMQKQPGQQHPNPPLLSGSLLITVSRSECEVDCKHTTVFGWRVSDGSPVWSYTLPGGYVKLTTTAGYAAVMINTLSTITMTSTNTMTVLDTTTGKPLWSRPVEGGSGFALDGKHAYVSRGSIRAYGIRDGEPAWTGADGGYNAVHTSPYGIFATSGDTTAAYDSTGRRRWWSRIHPRSTLTVDTGIVYFQQSPNGRARGAAYLAAMRASTGKLLSRTKISDGGSIGDVAVGGGRVFTKVLLERIVAFAPARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0P7</accession>
    <name>U5W0P7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bulb-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16195"/>
    <dbReference type="EnsemblBacteria" id="AGZ41521">
        <property type="protein sequence ID" value="AGZ41521"/>
        <property type="gene ID" value="AFR_16195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3286"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1076177_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043992">
        <property type="entry name" value="SLT_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18896">
        <property type="entry name" value="SLT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="SSF51110"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665557" description="Bulb-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="2">
        <location>
            <begin position="27"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="26749" checksum="3822D83DE525F669" modified="2014-01-22" version="1">MAAAGVVTALLTGTAVVTAGPAQAASADRLTPGRTLTPGAELRSANQRYRLVMQTDGNAVVMTAGGTPLWSSDTPGYGGAALEMQNDGNLVLYKSGHVALKASGTAGRQSASLVLQDDGNLVVYTSDGRPWWSTNTSGGAKAPAPTGARLCARVGYYAGFREQGLQLAVQVALAESGCNPAATNRNTNNSVDYGLWQVNNRAHPQWTPSQLLDPQTNANAAWSISGRGGNWRPWVAYTSGAHTRNGIPAKANAAVTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0S6</accession>
    <name>U5W0S6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22940"/>
    <dbReference type="EnsemblBacteria" id="AGZ42858">
        <property type="protein sequence ID" value="AGZ42858"/>
        <property type="gene ID" value="AFR_22940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4649"/>
    <dbReference type="eggNOG" id="COG5485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271337_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436">
        <property type="entry name" value="PTHR38436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07366">
        <property type="entry name" value="SnoaL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="102" mass="11095" checksum="3B25C4C2CADAE667" modified="2014-01-22" version="1">MKQPNGVAQIGEFRRAVPDYDVVHEDFVVGEDRVAVRNTVSGTHQGAFLGFEPTGRRITMRTLDIHQVRDGRIVATWHLEDYGGLLAQLSAPAGAAVPGAWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W109</accession>
    <name>U5W109_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3',5'-cyclic-nucleotide phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361651.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16470"/>
    <dbReference type="EnsemblBacteria" id="AGZ41576">
        <property type="protein sequence ID" value="AGZ41576"/>
        <property type="gene ID" value="AFR_16470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3340"/>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063034_0_0_11"/>
    <dbReference type="OrthoDB" id="1242748at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="29423" checksum="F0CE68CD90269D7B" modified="2014-01-22" version="1">MRLAHLTDLHFGAEVPEVVAALRDDLAGRDLDHVLVSGDLTMRARAGQFRAARELLESIGHPWTSVPGNHDLPLFRVLTRAFRPLSAYRKGVGRRAEPELGSGGLQVLGLSSPKRYLSRNGRIDAAQVARIGTAFTGPAGLRVLMLHHPVFRPAQRPGQPLVRGAEQALRAAARAGVDVILCGHDHVQAQADLALTWPGLGRHMIAVASGTACSRRTRAGESQSYTLLELTGDTLSVHVRHWNDGRFETLSETTWQRGADGWLPARAACRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W188</accession>
    <name>U5W188_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acetaldehyde dehydrogenase</fullName>
            <ecNumber evidence="3">1.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Acetaldehyde dehydrogenase [acetylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_23750</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>acetaldehyde + CoA + NAD(+) = acetyl-CoA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:23288"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the acetaldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363608.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23750"/>
    <dbReference type="EnsemblBacteria" id="AGZ43018">
        <property type="protein sequence ID" value="AGZ43018"/>
        <property type="gene ID" value="AFR_23750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4810"/>
    <dbReference type="eggNOG" id="COG4569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062208_0_0_11"/>
    <dbReference type="OrthoDB" id="1432332at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008774">
        <property type="term" value="F:acetaldehyde dehydrogenase (acetylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01657">
        <property type="entry name" value="Ac_ald_DH_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003361">
        <property type="entry name" value="Acetaldehyde_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015426">
        <property type="entry name" value="Acetylaldehyde_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09290">
        <property type="entry name" value="AcetDehyd-dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015689">
        <property type="entry name" value="Actaldh_dh_actl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03215">
        <property type="entry name" value="ac_ald_DH_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="2 3" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="4">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="160"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="31699" checksum="83E3CD4910792C6C" modified="2014-01-22" version="1">MTRKKVAVIGSGNIGTDLMIKVLRRSAVLEMAAMVGIDPDSDGLARAARLGVPTTAKGVDGLIALPGFADIAVVFDATSAAAHRANAARLAPYGKTLIDLTPAAIGPYVVPAVNLEEHLGAGNVNMVTCGGQATIPIVAAISRITPVAYAEIVASIASRSAGPGTRANIDEFTETTAHAIETVGGARRGKAIIVLNPAEPPLIMRDTVHALIGEPSTHDAVRESIERMVASVAEYVPGYRLKQPVQITAVPPDQPVATLLADSSGIPPTHQVSVFLEVEGAAHYLPAYAGNLDIMTSAALRMAERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1N6</accession>
    <name>U5W1N6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362264.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18085"/>
    <dbReference type="EnsemblBacteria" id="AGZ41895">
        <property type="protein sequence ID" value="AGZ41895"/>
        <property type="gene ID" value="AFR_18085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3670"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_0_1_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="30"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="28053" checksum="8342633EAE0B1ACA" modified="2014-01-22" version="1">MEKADVDRTLTLLWRRTLGVPQGSRGPRQRVSVDEVVAAAVAVADEEGLPAFSMRKVADRLGIKLMSIYTYVPGRAELIGLMVDEVYGEADLTPHTGPVRERMATVARLLWDEYHRHPWLLQIDETRPWIGPNGSDRYEWQLAAIEGAGFTDLEMDQVVTLLSGFAASSARLSIDSRRTEQRSGITDAQWWEVNAAVLERVMPPDRYPLSGRVGTVAGKEYNAVADPGRSFRFGLDRLLDGLELMLEQRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Q5</accession>
    <name>U5W1Q5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17890"/>
    <dbReference type="EnsemblBacteria" id="AGZ41856">
        <property type="protein sequence ID" value="AGZ41856"/>
        <property type="gene ID" value="AFR_17890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3629"/>
    <dbReference type="eggNOG" id="COG3222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1486024_0_0_11"/>
    <dbReference type="OrthoDB" id="1445278at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018641">
        <property type="entry name" value="Trfase_1_rSAM/selenodom_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09837">
        <property type="entry name" value="DUF2064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="181" mass="18568" checksum="32E79477FF81F319" modified="2014-01-22" version="1">MEFVTDLAGPAGRLCTDFSAEQATMLSLAAAADTIRAVQAMPGWAAATGSLAEVFAGDRRWPGPVLLIATEIPQLGPDLLVCATDLLRDFDAVLGPTTGDGWWAFGLREPARVALGPLTGLGLLNGLGSLTVAALRLGLRVAMLPTLRGLGSGADVRPVAAHCPAGSMFATTVAQLTGRTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W209</accession>
    <name>U5W209_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proline/glycine betaine ABC transporter periplasmic component-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18440"/>
    <dbReference type="EnsemblBacteria" id="AGZ41966">
        <property type="protein sequence ID" value="AGZ41966"/>
        <property type="gene ID" value="AFR_18440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3742"/>
    <dbReference type="eggNOG" id="COG2113">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072064_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007210">
        <property type="entry name" value="ABC_Gly_betaine_transp_sub-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04069">
        <property type="entry name" value="OpuAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="OpuAC" evidence="2">
        <location>
            <begin position="35"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="34247" checksum="7F49590DD9814879" modified="2014-01-22" version="1">MTAAVAVLTLGLAGCGGATSDAGTDDKPAAGSKGTVKLAINPWVGYEANAAVLGYLLEHELGYTVDKKNLKEEIAWQGFETGEVDAIVENWGHDDLKKTYITDKKTAVDLGPTGNKGVIGWYVPQWMADKYPDITDGKNLNKYADLFKTSESKGKGQLLDGDPSFVTNDKALVANLKLNYEVVFSGSEAAIIKAAQQATAQKKPLLFYFYEPQWLFAKEKYARVKLPAYTAGCDADPKKVACDYPDYILDKIASKKFADTNSPAYQLIKNFTWTNDDQNLVSDYITNQGMTAEAAGEKWAKEHEATWKPWIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W216</accession>
    <name>U5W216_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_24440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363744.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24440"/>
    <dbReference type="EnsemblBacteria" id="AGZ43154">
        <property type="protein sequence ID" value="AGZ43154"/>
        <property type="gene ID" value="AFR_24440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4951"/>
    <dbReference type="eggNOG" id="COG4941">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035311_1_0_11"/>
    <dbReference type="OrthoDB" id="1469807at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="16"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="119"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="419" mass="45201" checksum="50DD09FE48AFDAFA" modified="2014-01-22" version="1">MPDEWTAGASVEAVFREERGRLLAALVRRFGDLDLAEEVTSEAIEAALTHWPAQGVPPAPGAWLMTTARRRAVDRLRRDQALASRLAILQVEADRAGPVPAVEPGDDLPDERLQLFFTCAHPALAAEDRGALILRFLAGLTTPEVARAFLVPPATMAKRITRAKTKIRDARIPFRVPGADELPGRLPGVLQAIYSLFTEGYAASSGPHLQRPRLAEEAIRLARILHRLLSGERETAGLLALMLLVHARRDARTGPDGAPVLLDEQDRSRWDAPMIAEGSALAAASLTGGPPGPYGVQAAIAALHDEAPHLDATDWEQIVALYDVLLALTPSPVVALNRAAAVAMRDGPEAGLELLDELADEPALRGHHPYPAARADLLHRLGRDAEAAAAYREALDLAGTGPDREHLRRRLDLLDRPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W256</accession>
    <name>U5W256_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42040">
        <property type="protein sequence ID" value="AGZ42040"/>
        <property type="gene ID" value="AFR_18820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18820"/>
    <dbReference type="HOGENOM" id="CLU_1987852_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="12626" checksum="5D4933F8DD8D3388" modified="2014-01-22" version="1">MRCSRPSWRRRNARLSRSDFPLSDRYEPSRAVPGSNGTGPPRRGAAFAGFGDGDALFDGLGDGFADGLAEVADGVGEAVVGSAAAGDGGAGSAEQAAGRVSAARASTERRGSRRPPGFSAVMSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2B6</accession>
    <name>U5W2B6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signaling protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25595"/>
    <dbReference type="EnsemblBacteria" id="AGZ43383">
        <property type="protein sequence ID" value="AGZ43383"/>
        <property type="gene ID" value="AFR_25595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5188"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="198"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="348" mass="37020" checksum="223FC2EA3ED4F719" modified="2014-01-22" version="1">MHGASRAVAYLTLAACPYVLFTALLTPGIDLPGVIAVVAVSMLMGVAGLICWRWPGIFPRLFWFGAPLVATLSISGLNLATSDASTGAQLFYLWPILFAANFLSRRVVYANLLIVFAGHAATVFSILNFQRAAADWTAMVLAMTMSAFVVLSLRRRADKLRDVLEQQAHADHLTGLANRRHFDEALLREGESARRTDATLALITVDLDHFKAINDTFGHTEGDRVLQAVATAMRTVVGDSGLAARLGGDEFVILMRVGRRAAVGVAEALRAAVAARTDLPGGPPGLSIGIAVLPGDVSTVQDLVAASDAALYDAKTSGRGRLSVAGARQNVDQMPSFAPDPLVPDQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2C9</accession>
    <name>U5W2C9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoadipate:succinyl-CoA transferase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_19290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362502.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19290"/>
    <dbReference type="EnsemblBacteria" id="AGZ42130">
        <property type="protein sequence ID" value="AGZ42130"/>
        <property type="gene ID" value="AFR_19290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3918"/>
    <dbReference type="eggNOG" id="COG1788">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049557_0_0_11"/>
    <dbReference type="OrthoDB" id="1660857at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28733" checksum="336572465FB4F5F0" modified="2014-01-22" version="1">MTARIVSLAEAVAELVHDGDTVALEGFTHLIPVAAGHEIIRQRRRGLTLVRMTPDIVYDQLIGAGCAAKLIFSWAGNPGVGSLHRFRDAVQHAWPVPLEIEEHSHAGMANRYVAGASGLPFAVMRGYSGTDLPAHTATVAPIQCPFTGEMLTAVPALNPDVGIVHAQRADRRGNVQFWGITGVHKEVVLASARSLVTVEEIVDELTPMPGAVVLPGWAVTRVALVPGGAHPSYAMGFSERDNDHYQAWDAISRDRDTFGQWLTEVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2E7</accession>
    <name>U5W2E7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_19390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19390"/>
    <dbReference type="EnsemblBacteria" id="AGZ42150">
        <property type="protein sequence ID" value="AGZ42150"/>
        <property type="gene ID" value="AFR_19390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3936"/>
    <dbReference type="eggNOG" id="COG2182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_17_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015144">
        <property type="term" value="F:carbohydrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006060">
        <property type="entry name" value="Maltose/Cyclodextrin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00181">
        <property type="entry name" value="MALTOSEBP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666018" evidence="4">
        <location>
            <begin position="30"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="422" mass="43766" checksum="F564162774BFD602" modified="2014-01-22" version="1">MEKIMRIRTPGVVLAVAALALTAGCSSGGDDPQNSTSATPAGATGKLVIWGDDKRAAALKPFADKFGQENGVTVEVQAISKDQQTTFVTASQQGSGPDVMVGAHDWIGNLVQNGAIDPVQLTAEQQQGLAPNALKAVTFNGQVYGVPYATENLALIRNTKLAPEAPATIEDLVKNGKKLKADKKASEILCLQVGQNGDAYHAYPLFTSAGGYLFGTDASGAYDKNDLGVGKPESIAAFAKIRALGEKGDGALKRSITVENSIATFTSGKCAYLVSGPWAVTDAKKAKIAYDISPVPGFAGGKPAQPFLGVQSFYVAAKGKNKALAQEFVTNYVTTPELAVALYQAEPRPPVLTAAFDQVKGTDPDLAKFVDAGKNAVPLPAIPEMATVWDPFGKAESAVIGGADPKAALTAAAKTISGQIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2J3</accession>
    <name>U5W2J3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24830</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363822.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24830"/>
    <dbReference type="EnsemblBacteria" id="AGZ43232">
        <property type="protein sequence ID" value="AGZ43232"/>
        <property type="gene ID" value="AFR_24830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5030"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_357776_0_0_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="420"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="529"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="4">
        <location>
            <begin position="179"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="636"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="746"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="783" mass="82050" checksum="CF9E11B2ABE85C28" modified="2014-01-22" version="1">MKRIPVWLLPLALAVVQLAVWPGVPLLLDDRLSGLAVATGVTLTLVVAGALLWRRSSPLTVLAIVVVAITVGTFGVPQDALLTISLADLIVLFGVAVLRPARLTFGVTAGVIAWQSVLIVVATADTDPEYPFEIAMVVVVYATVVAFGRARRRWHRERAEAAVRLAEAEDRRAQAADTERHRLARELHDVTAHHLTSIVVIASAAQRLGDTRPDLVAEARDFAARTGRETLDALHRLVALLRLPDERPGPAALRLAELAEGFRLLGQQVTVRTDPGELPSPATEAAVGITREALTNTLRYAPGSAVRIELTAVPGGTELIVDDDGARAEGVADLGSGRGVAGMRERATALGGTLTAGPRAGGGWRVRALLPQAGAVAAVRRRWRPGAEHVIDFFVLGLVLLLPVGSLLVDDDPDVDQASLPLLLLLVLAHAAPLVWRRRRPWGVLAVVAATSWAWPALIVADLVAPSLGWVMLTGLSAETAAVYAVARYGRRPGLSGLSIPAALVPMALATGATLALDPGPDDAGVPLAAVIVIMAIVTGIPFGLTVTAAWLAGYLARRRHTRVLTQEYDAVAASTFHAIAAAGAERARVAAGLREAVLRDTERVATAAADGDLDQVLESARAALDAMRGLLNGLREEPDANRDAQGRDPQPTTAALPGLADRWRGGGRQVHLDIRGAGRKLPADVDLSAFRVLELLLAGDTGPVAVWADLTGDPLRLAVRPMPPDEGGEVSAGLRARLAAVGGSMGTAADGQPEILLPAPHPDGTRPVEATADEEVASSPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W309</accession>
    <name>U5W309_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ricin B lectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364290.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26260"/>
    <dbReference type="EnsemblBacteria" id="AGZ43514">
        <property type="protein sequence ID" value="AGZ43514"/>
        <property type="gene ID" value="AFR_26260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5322"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_484546_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665646" evidence="1">
        <location>
            <begin position="26"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="415"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="562" mass="59776" checksum="2D1A6A13D45CCD59" modified="2014-01-22" version="1">MRRLHLFLAALVVALVPAIAAPAQAAAVTGRAVKIMPLGDSITWGTGSMNGSIPGSGYRAGLWTKLVAGAGLAVDFVGSMNSGSLPDTDNEGHSGYRIDQITANVTGWLTAAQPDVILLHIGTNDFWQDHETSTASKRLGLLLDKIHQVRPSATLIVAEIVPTRQDALNTKIDAYNAAIPGLVATRAAAGHNIRSADLHDTMTDADFADTLHPNNTGYEKMANLWHSALEPVLGGGREWPLLRTGLEPGETAPTWIDTVDRASGVGGYQAGLTTMETGPRTGTSHGGANALMYSGNDQSTSLSYSYMRVFDTHLRISPSSVLSYWIRPQQTNGTYVAVGLLFTDGSSLRDSTAVDQYGVRAHPLHQGQGGHLVVNQWNLVTVSLSALAGRTVDRVQLGYDQPAATGVFRGFVDDIAVTDHGLPAQNLALDRPATATTEPCAETETPAQATDGVTTTNSKWCTRAAGQWQVDLGTASPVGRVVLRHASAGGESLAWNTRAYHVETSEDGETWTAFATVTDNADGITTSSADPVTARHVRVTVDAGRQDEYPLTRIYEVEVYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5W335</accession>
    <name>U5W335_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42405">
        <property type="protein sequence ID" value="AGZ42405"/>
        <property type="gene ID" value="AFR_20665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4190"/>
    <dbReference type="HOGENOM" id="CLU_3163555_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="47" mass="5308" checksum="D7319053ACC973E8" modified="2014-01-22" version="1">MKRTLARAGDDPKKPPEGTIGWGQLTFDRPAGPAYHHPARHLFLGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3A7</accession>
    <name>U5W3A7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_26200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364265.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26200"/>
    <dbReference type="EnsemblBacteria" id="AGZ43502">
        <property type="protein sequence ID" value="AGZ43502"/>
        <property type="gene ID" value="AFR_26200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5310"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_2_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025828">
        <property type="entry name" value="Put_sensor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13796">
        <property type="entry name" value="Sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Sensor" evidence="3">
        <location>
            <begin position="29"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="224"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="413" mass="44175" checksum="4B088C08E1DDD168" modified="2014-01-22" version="1">MPVRNVLDALTMRPTRFLTSSWPWRSLAYLTGGALIGIATICAVVVLLAAGLVLSVVVVGIAGYVATVLSGVAVGRLERWRMRLVDHAELPDPHRSPPAPGLRSWLRCRLREQATWRELGYTMLSALVLCWMDAAVVYAAVFVPTSLLFAPYYLYGETSLWTTVPLALCTPFALAVGAYPVTAWAGARAAMARALLAPRSDDTDAKLVELTRSRARLVDAFEVERRRIERDLHDGAQQRLVALSMQLGLARLELPAGSPAETQVAAAQDLAKEALSELRELIRGVHPKVLTDRGLAAAVGEAAGHAPVPVDLDLRLDRRLPDAVEVTAYFVVVEALANVAKHSEATRAAVSAGLIGERLHLDVRDDGRGGADPDRGTGLVGLADRVAAVDGTVALSSPPGGPTLVHVEIPCAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3D9</accession>
    <name>U5W3D9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Collagen like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26810"/>
    <dbReference type="EnsemblBacteria" id="AGZ43624">
        <property type="protein sequence ID" value="AGZ43624"/>
        <property type="gene ID" value="AFR_26810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5435"/>
    <dbReference type="eggNOG" id="COG5295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_511572_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="532" mass="51268" checksum="7A2A818581BB4E59" modified="2014-01-22" version="1">MGQPSQSGQPSRFGQPGQSGEPAKTSQLGHLGQSGQVGALGERGQSHATSQPGQGDQLGQAASAGQWDDAGHLGRSAPTGQPSEPHQQGHFGQSGLGQPVQGGRPGHAGQQDGLGQPGSGGQGSPAVHANQLGQPRESGSPGQPGSLGQPGTANQPGSPGQPGTANQSGSLGQSGSLGQPGSLGQPGSLGQPGSLGQPGSLGQPGQPGQPGQPGQSGESGNLGQSGQPVQPGQSGQHDVSGPSAQPGPFAQQGQSAAAGQPGQASPAGVDGHFATRAQAADPVASAFARPAEADRGAASGVEGQFAARAQAADPVASAFARPAEADRGAASGVEGQFAARAQAADPVASAFARPAETARGAGSGVEGAGPSRAPDPVASAFARPGAQPAPTGQFGQAAQQGEARPSPAGAPARPPLEPPQLGQLNRPVEPEGQAPAGQFQAGQAGAGQDRAGQGPAAQGPGATAAETQFLATPAHHFAPPAGGARGQEQGESAPEDPGVTPSGLPSRSVVPPAAPRNGTVDEEGPSGLPRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Q1</accession>
    <name>U5W3Q1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362972.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21650"/>
    <dbReference type="EnsemblBacteria" id="AGZ42600">
        <property type="protein sequence ID" value="AGZ42600"/>
        <property type="gene ID" value="AFR_21650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4388"/>
    <dbReference type="eggNOG" id="COG3287">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052774_2_0_11"/>
    <dbReference type="OrthoDB" id="1942718at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019494">
        <property type="entry name" value="FIST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013702">
        <property type="entry name" value="FIST_domain_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08495">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10442">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00897">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01204">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIST" evidence="1">
        <location>
            <begin position="31"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="FIST_C" evidence="2">
        <location>
            <begin position="228"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="388" mass="39414" checksum="BC913E6DA3CD3B14" modified="2014-01-22" version="1">MERWFGVGRSLHSDPATAGAEAARDAVAGRRPGLLVVFASLPYSTPPMAEAAHGAAGGDVPMIGCSTSGEFTQDGRGESVVVLALGGPGFEASVRAVPERATGLREAGLEAAACLDDIDREHRMVLLLGDGRSSDQQEMVRGAYAHTGAGVPLIGGCAGDGMTQTSTRHFFSAGTGVSVLTNAVLAAAVGSDAPIGIGLAHGWRKTGEPMVVTRSEGGKIFELDGERALDVYLRRSGASPDLVDDPMGFFGFATVHPLGLSRRTGEDLRVIFQADAAEGSISGLADTPEGAMAWYMEADPEAVTGAAAQAAAEAVAALEGADPLGVFIFDCCVRGLALGPDGTDAAGKQLGETLGPVPFGGFVSNGEIVRTAGAKGMHHLSVAALAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Q8</accession>
    <name>U5W3Q8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362982.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21700"/>
    <dbReference type="EnsemblBacteria" id="AGZ42610">
        <property type="protein sequence ID" value="AGZ42610"/>
        <property type="gene ID" value="AFR_21700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4398"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="176"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="373" mass="39386" checksum="CAAF0E527D34B983" modified="2014-01-22" version="1">MGRVRDWMLAAGTAALLVVTGWSGHHPPTGTGTDLTVTDLAGFVLLAAGGLALVARRRHPVAVLVVTGLCAVGYEIAGFTVLTIAYLVAVYAAVRAGHRTTTVVISVILLVVLPVAAMTAGRQDPGEAFAQARGVLELAWLIAAGAAGEALRQAERRADVAERTREQSALLRADEERLHIARELHDSLTHQISVIKVQAEAAVHVAHKRGEEVPEALLVIRDAGREAARELRATLETLRSDDRSPLHGLDQVPELVRRARSAGLDASLTVEGHPGEVPAAVDRTAYRIVQESLTNVTRHAAAATASVRIGYRPGTVDVRVDDNGVATAGTPPVPGVGLLGMRERVTALGGRLRAEPRAEGGFTVHAELPWDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3R1</accession>
    <name>U5W3R1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560112.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27565"/>
    <dbReference type="EnsemblBacteria" id="AGZ43774">
        <property type="protein sequence ID" value="AGZ43774"/>
        <property type="gene ID" value="AFR_27565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5587"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_5_3_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022315">
        <property type="entry name" value="F420_OxRdatse_CPS4043_pred"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03842">
        <property type="entry name" value="F420_CPS_4043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="36406" checksum="57FECE607801A977" modified="2014-01-22" version="1">MDIGVVLQCDPPALEIVEWAKKAEDLGYSHVWTFDSHVLWQEPFVIYSKILADTERVIVGPMVTNPGTRDWTVIASQFATLNEMYGNRTICGIGRGDSALRVLGLQPNTLGQLRESIGVIKGLASGEQVTLRGQELQFAWAPESRLDVWVAAYGPKALALTGEVGDGYILQLADPDIAAWMIKAVRDAAEKAGRDPMAIKFCVAAPAYVGDNLEHQRDQTRWFGGMVGNHVADIVARYGGDGAVPQVLTDYIKGRQGYDYAEHGRAGNSHTSFVPDEVVDRFCILGPIENHLKRLEELRAIGVDQFALYLQHDDKDATLRAYGEKIIPVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Y0</accession>
    <name>U5W3Y0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21730"/>
    <dbReference type="EnsemblBacteria" id="AGZ42616">
        <property type="protein sequence ID" value="AGZ42616"/>
        <property type="gene ID" value="AFR_21730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4403"/>
    <dbReference type="eggNOG" id="COG5446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090632_0_0_11"/>
    <dbReference type="OrthoDB" id="2061199at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012666">
        <property type="entry name" value="CbtA_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09490">
        <property type="entry name" value="CbtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="27499" checksum="2E5321814DB0C84C" modified="2014-01-22" version="1">MSLTRTRTTPSYGAILLRGLLAGLIAGLLAGVFAYSTGESRIDAAIAIEEAAAADPGVHAADDGHHDEELVSRTGQKGGLFLATALYGVAMGGLLATAYTLLRRRLRTPDDTRAAFGLAGAALVGVVLVPFLKYPANPPAVGDPATINQRTISYLSIVVIGLVAVWAGVVASRSLRSTAPEWLRAIAAVAGFLIVVTVGYVLLPSIDEVPDTFPPSLLWNFRISSLGTQATLWVALGLAFAALLSRLGARPPAVEREKSETVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4A3</accession>
    <name>U5W4A3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Selenide, water dikinase</fullName>
            <ecNumber evidence="5">2.7.9.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Selenium donor protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Selenophosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">selD</name>
        <name evidence="8" type="ORF">AFR_27955</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Synthesizes selenophosphate from selenide and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + hydrogenselenide = AMP + 2 H(+) + phosphate + selenophosphate</text>
            <dbReference type="Rhea" id="RHEA:18737"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:29317"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the selenophosphate synthase 1 family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.3" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27955"/>
    <dbReference type="EnsemblBacteria" id="AGZ43852">
        <property type="protein sequence ID" value="AGZ43852"/>
        <property type="gene ID" value="AFR_27955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5666"/>
    <dbReference type="eggNOG" id="COG0709">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032859_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004756">
        <property type="term" value="F:selenide, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00625">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023061">
        <property type="entry name" value="SelD_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="PTHR10256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036407">
        <property type="entry name" value="Selenphspht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00476">
        <property type="entry name" value="selD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0711">Selenium</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AIRS" evidence="6">
        <location>
            <begin position="54"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="7">
        <location>
            <begin position="173"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="143"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="5">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="34340" checksum="8EE4543FC18510F7" modified="2014-01-22" version="1">MTSIQEKRLTAYAHGGGCACKIPPGELEQVVSGLMGESAPRGPGELLVGLDDGDDAAVVRIAAGVAVVATADFFTPVVDDAFDWGRIAVANALSDVYAMGGAPVVGVNLLAWPRDLLPMELAAEVLRGGLAVAREAGCHVAGGHSVDDPEPKYGMAVTGVADPDRLMRNDAGRPGVPLTLTKPLGIGVLNSRHKTTGEVFPQAVAAMTELNRAASEAALAAGAVCATDVTGFGLLGHLHKLARASKVTAVVDAAAVPFLDGARDALRDGFVSGGTRRNLDWVRPHLDGGAVSEDELLLLADAQTSGGLLVAGEVPGHPVVGELVPARQDGVTLVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4B8</accession>
    <name>U5W4B8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_28615</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560321.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28615"/>
    <dbReference type="EnsemblBacteria" id="AGZ43984">
        <property type="protein sequence ID" value="AGZ43984"/>
        <property type="gene ID" value="AFR_28615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5802"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_11"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="308"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="366"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="582" mass="62223" checksum="A56504081342126B" modified="2014-01-22" version="1">MSRSVPVHRSLVVRLLGTSVLIVVLAVATAAWLAAKTATRAVQEERGQSLAGDSQIYDTLLGFAAGHRSWNGIELTVRSLSEETGRQITLTRVDGTPIAASHPGVALPDRPTAVVDPLGGGTGVIDARAVGPYRLPERDRAAQRDLAEDRLDCLRDQGFQATLQTTPSGRSVVRMADRNPRLSEECSFREDSPTTTEREALEKLKYLVNPCLKDRHLPEIDFYSADFTWQYHAQNIGGSTQDIQDCVDNARRMQLRPYVAPPARLYLSIPARAPTPVFDLSAASTLRIAGATAFVLLVAVAATLLVGLRLVRPLRALTAAADSATHRPAPVTIARNDEIGRLATALNDLAERSERGEDQRRTMVNDIAHELRSPLTIIRGWLTAGQDGLADLDQDLVRLLLEESGLLQHIVDDLADLADADAGTLTLTPGDHPIADLLAQVAEAHRAAADRGGVDLRVTVAAGTRAVVDPVRFRQIVGNLLANAIRHTPAGGAVEVRARLETGDLLVEVADSGEGIGASDLPHVFDRFWRADKARGRATGGSGLGLSIARRLSQAHGGELTAFSTVGVGSVFTVRLPAPGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4E4</accession>
    <name>U5W4E4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560395.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28985"/>
    <dbReference type="EnsemblBacteria" id="AGZ44058">
        <property type="protein sequence ID" value="AGZ44058"/>
        <property type="gene ID" value="AFR_28985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5873"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_2_0_11"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="5"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25065" checksum="EB1341A20E8F4F97" modified="2014-01-22" version="1">MKPTVILVHGAYAESSSWTGVLEPLVTAGHRVIAFATPLRGVATDAALLSELVATIEGPVLLAGHSYGGAVITNITADPGHVIGAVYIAGFALEPGESCADASALAPGSTLAETLVPVPSAAGGADTYIAAEKYHHQFAADLSVEQARLMAITQRPVTDRALAEPSGDQPLWQSVPSWFLFGELDHNIPAGAHRIMAARAKARNTVEIAGASHVVGISHPAETAQIILEAAGSSGTESEGQRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4H0</accession>
    <name>U5W4H0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cinnamoyl ester hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560425.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29135"/>
    <dbReference type="EnsemblBacteria" id="AGZ44088">
        <property type="protein sequence ID" value="AGZ44088"/>
        <property type="gene ID" value="AFR_29135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5902"/>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_2_3_11"/>
    <dbReference type="OrthoDB" id="2057904at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="13293" checksum="AD682DFEB7BF0D49" modified="2014-01-22" version="1">MATLAQPKSGITVFDVYAATCPSRALLDLVTSRWAVLVIGALEEHPQRFGALRRRLEGVSQKVLTEKLRDLEAEGLISRTVVDRPLAVYYELTPIGRTLVQPLAALRDWAQTHCDGHQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4K4</accession>
    <name>U5W4K4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363377.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22980"/>
    <dbReference type="EnsemblBacteria" id="AGZ42866">
        <property type="protein sequence ID" value="AGZ42866"/>
        <property type="gene ID" value="AFR_22980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4657"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_29_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="228" mass="24956" checksum="1228EDD71A253172" modified="2014-01-22" version="1">MARSVNERDHAARRDAIISAAYRLIATKGYQQMTVQDLLDDLTISKGAFYHYFDSKPALLDAFIDRVITAYESQLVPVASDGGQPPLERLRGSLVVMCGARICDRAFLAALRVLHSDDNAVVMQRSRVAGIRRFGPLLAPILAEGAAAGVFRVDRPEAVAAALVTLVQDLVDAAGRLLLDRADWPAVDAVVSVYTEIVERTLGLPPGSVELLDRRLLEQWVEEHHGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4U6</accession>
    <name>U5W4U6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amidohydrolase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560476.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29390"/>
    <dbReference type="EnsemblBacteria" id="AGZ44139">
        <property type="protein sequence ID" value="AGZ44139"/>
        <property type="gene ID" value="AFR_29390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5957"/>
    <dbReference type="eggNOG" id="COG3618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044590_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="PTHR21240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="29643" checksum="590145EB8A454263" modified="2014-01-22" version="1">MRIIDAQIHLWASGTAPAHHWRSPFTVERALADMQAAGVSRAVNCPPLWDPAADEYADRAVLDHPDRFATMGGFPLDATADASSVDRALSRPGLLGLRFILPTPELGDRLASGALDWLFSAAHERQIPVALIVLPRYLPAVADIAGRYPRMRLVMDHLALSPFEKLPQAAAHFDDLLALARRPNIAVKATGVPGTATDPYPYPGTHELIRRAFNAFGAERVFWGTDITRLPCTWPEAVTMFTDELSWLKGRDLELVMGDALAAWIGWHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Y1</accession>
    <name>U5W4Y1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MbtH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363591.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23665"/>
    <dbReference type="EnsemblBacteria" id="AGZ43001">
        <property type="protein sequence ID" value="AGZ43001"/>
        <property type="gene ID" value="AFR_23665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4795"/>
    <dbReference type="eggNOG" id="COG3251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181321_1_0_11"/>
    <dbReference type="OrthoDB" id="2067093at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005153">
        <property type="entry name" value="MbtH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038020">
        <property type="entry name" value="MbtH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037407">
        <property type="entry name" value="MLP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38444">
        <property type="entry name" value="PTHR38444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03621">
        <property type="entry name" value="MbtH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00923">
        <property type="entry name" value="MbtH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160582">
        <property type="entry name" value="SSF160582"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MbtH" evidence="1">
        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="68" mass="7640" checksum="64DF60CC84EF3419" modified="2014-01-22" version="1">MSTNPFDNAEGMFLVLVNDENQHSLWPVFAEVPAGWRTTHGPAGRQDCLDHINTTWTDMRPQSLIDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Z1</accession>
    <name>U5W4Z1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TrkA-C domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30115"/>
    <dbReference type="EnsemblBacteria" id="AGZ44283">
        <property type="protein sequence ID" value="AGZ44283"/>
        <property type="gene ID" value="AFR_30115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6100"/>
    <dbReference type="eggNOG" id="COG0490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116143_1_0_11"/>
    <dbReference type="OrthoDB" id="941006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026278">
        <property type="entry name" value="K(+)/H(+)_antiporter_KhtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005028">
        <property type="entry name" value="KhtT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK C-terminal" evidence="1">
        <location>
            <begin position="73"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="16821" checksum="3572CBDDC07EE2BC" modified="2014-01-22" version="1">MTIERTALPGVGISHSVTTAAQQRLGVVSHLSGRRDLVVYDTEDPEQAARTVTLEDHEAHQLAGLLAGTVTVDHVGELERLIAGISAVRLRIPAGSAYDGVALRETRARTHTGVSVVAVERDDEVIPAPDPGFVLRHGDTVVAVGDDPGIVALTALIAAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W515</accession>
    <name>U5W515_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_29155</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560429.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29155"/>
    <dbReference type="EnsemblBacteria" id="AGZ44092">
        <property type="protein sequence ID" value="AGZ44092"/>
        <property type="gene ID" value="AFR_29155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5906"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_0_11"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032783">
        <property type="entry name" value="AraC_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037923">
        <property type="entry name" value="HTH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12852">
        <property type="entry name" value="Cupin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51215">
        <property type="entry name" value="SSF51215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="202"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="33995" checksum="0CE46E721AE30742" modified="2014-01-22" version="1">MDPLSEVLALLKPRSYITAGFDAGGDWALGLDDLAGRIKCYAVVEGSCWLTVDGEAPVLVPEGSCFVLPTGRVAVIGSSPDVVPARASTVLDPDRSGEVITYNGGGDVYLVGTRFEAGSPHVELLLQNLPPLMLVMTSDDRARLRWSIELMMEEFREPRPGSALMAQQLAHMMLVQALRLYLAAPAHDDVGWFAALADPRMHAALAAMHDEPARSWTVRELATLAGMSRSTFAERFRERAGETPIAYLTRWRMMLAADRLQAGDDTVARIAGSLGYESEHAFTTAFKRIMGMPPRRYAAHARQNGAGLPDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5H1</accession>
    <name>U5W5H1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Urea ABC transporter permease UrtB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_30990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560793.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30990"/>
    <dbReference type="EnsemblBacteria" id="AGZ44458">
        <property type="protein sequence ID" value="AGZ44458"/>
        <property type="gene ID" value="AFR_30990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6273"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_2_2_11"/>
    <dbReference type="OrthoDB" id="974216at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017779">
        <property type="entry name" value="ABC_UrtB_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03409">
        <property type="entry name" value="urea_trans_UrtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30680" checksum="01682CCF47F55463" modified="2014-01-22" version="1">MGTLNQLVIGASIGAVLLLASLGLTFTFGQMGVINMAHGEFIMAGAYTAYVLQPVFGSQAVVVALPVAFAVGGVLGLILERLFIRRFYGRPLDTLLLTFGLSLILQQVARDIFGAPNVQVTAPGWLTGGLDLGGVRMPYNRIFILVLAVGCVVLISLYLSKLKQGRRMRAVMQNRQLAAVSGVATGRVDQATFFIGSGLAGVAGVALTLIGPVGPTLGSAYIVDAFLVVVAGGLGQLRGAVIAAFALGVLNSFVEFWTANGASFAKVVVFAVIIGFLQFRPQGMFVLRSRALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5H4</accession>
    <name>U5W5H4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363756.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24500"/>
    <dbReference type="EnsemblBacteria" id="AGZ43166">
        <property type="protein sequence ID" value="AGZ43166"/>
        <property type="gene ID" value="AFR_24500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4962"/>
    <dbReference type="eggNOG" id="COG4978">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1684236_0_0_11"/>
    <dbReference type="OrthoDB" id="1748122at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="17755" checksum="B5FE44792967F549" modified="2014-01-22" version="1">MIEVSTRDVPELHVVTEERSVDQAALEAWLPGAMARVHQAGTEAGGVLGTSALPFVQRTGDDQSVFVVLYEGNPNEGPTSVEVCAPIADRAAAARTIPAHREAFVRVTKEQALTGQLGGVYEAVEKWISDQGLEVTAAPRETYWTDFGAAAPGDVVFDISFPCTGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5L3</accession>
    <name>U5W5L3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_30185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560634.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30185"/>
    <dbReference type="EnsemblBacteria" id="AGZ44297">
        <property type="protein sequence ID" value="AGZ44297"/>
        <property type="gene ID" value="AFR_30185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6112"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_11"/>
    <dbReference type="OrthoDB" id="1439189at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="3">
        <location>
            <begin position="32"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="337" mass="36732" checksum="C57396997082546D" modified="2014-01-22" version="1">MSSLPRQSTRDSSRRSGSPIRAVPDAVSSARIELRQLRYLVTLAEELHFGRAAAREHIVPSALSAQVQRLERAVGVLLVDRTTRHVALTPAGARFLIEARQILEHVDRAAFLAQGMALAAPTLRVGVLDEGYDAVRPVLRAVQARHPELEIHQVLAGVPEQCRLLADGRLDVGIGRLPGTAPEIAAEMFRLDPLGVLIPDEHPLARLPHIPVSALRDETLLLADEEQAPEFNAFVAEVCRSAGFFPKLFSGSVQTLRAAVDLVVQGRCVLCTSASTAGISHGTDLTWRPLGPSVPRYPWSILWRAQDPSRYAVALVHTARELSAERGWREAAGERAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6H5</accession>
    <name>U5W6H5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364333.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26370"/>
    <dbReference type="EnsemblBacteria" id="AGZ43536">
        <property type="protein sequence ID" value="AGZ43536"/>
        <property type="gene ID" value="AFR_26370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5345"/>
    <dbReference type="eggNOG" id="COG3502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042007_0_0_11"/>
    <dbReference type="OrthoDB" id="560982at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024479">
        <property type="entry name" value="DUF3866"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12982">
        <property type="entry name" value="DUF3866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="360" mass="37265" checksum="90742465EB226861" modified="2014-01-22" version="1">MVRWRSGTVSTVRREWRGALELDVTTEDGEVRALAYPSLTGTPEPGDRVLLNVGALVMGLGTGGYALVVALPDRLPADPPTTGTDRDAGHLVKARYTPLQPILLGVDEEASPHRAVMAEADTLDGMPVVTADLHSALPAILAGIHADRPDAKVAYVMTDGGALPAWFSRTLDGLRNHLAGTITTGQSFGGDLEASTVHTGLLAARHVLQADVAIVAQGPGNLGTGTTWGFSGVALGEAVNATVALQGTPVASLRISDGDGRPRHRGVSHHSLTAYGRVALVPADLVIPDDLPVDLAKEVADALAPLTTLHRVVEVPTAGLDAALQASPVKLSTMGRGLTEDHAYFITAAAAGRHAATLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W785</accession>
    <name>U5W785_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Propionyl-CoA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_27725</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560143.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27725"/>
    <dbReference type="EnsemblBacteria" id="AGZ43806">
        <property type="protein sequence ID" value="AGZ43806"/>
        <property type="gene ID" value="AFR_27725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5619"/>
    <dbReference type="eggNOG" id="COG0365">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_5_11"/>
    <dbReference type="OrthoDB" id="141801at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACAS_N" evidence="3">
        <location>
            <begin position="4"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="64"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="511"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="625" mass="67070" checksum="E2CA462CC8690C48" modified="2014-01-22" version="1">MGDYRQAYDRSLRDPEGFWREAAEAIDWDVPPGRILDDSDAPLYRWFPGARLNTCHNALDRHVDAGHGDRPALIHDSPVTGQQRTFTYAELRDEVALFAGALRRLGVGPGDRVVIYLAMVPEAVIAMLACARLGAVHSVVFGGFGAPELAARIDDARPVVIVASSCGVEASRVVPYKPLLDRALGLAAHQPAHCVILQRPQLVASLEPGRDLSWSDAVAGASPAECVPVAATDPLYILYTSGTTGRPKGVVRDNGGHAVALRWSMDAIYAAGPGDVFWAASDVGWVVGHSYIVYAPLLTGATTVLYEGKPVGTPDAGAFWRVIAEHRVNVMFTAPTAIRAVKKEDPTGKLIEGYDLSSLRLLFLAGERLDPETYTWAGERLGVPVIDNWWQTETGWPIAASPRGLEDLPTRPGTPSVPVPGYRVRVVDPSGADVPAGQDGAIVIGLPLPPGCLPTLWQDDERYVRSYLSAFPGNYLTGDGGRFDADGYLYVMGRTDDVINVAGHRLSTGAMEEVLAAHPAVAECAVIGVHDPLKGQSPRGFVVLKSGATITEDQLRTELVAMVRDRIGPVASFRRVDVVPGLPKTRSGKILRRTMRGIADGVPEPVPSTIEDPAVLDALRPILTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W797</accession>
    <name>U5W797_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_33120</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561219.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33120"/>
    <dbReference type="EnsemblBacteria" id="AGZ44882">
        <property type="protein sequence ID" value="AGZ44882"/>
        <property type="gene ID" value="AFR_33120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6702"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_4_11"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="36246" checksum="830141C0992BFF1E" modified="2014-01-22" version="1">MTKRLAEVAKKAGVSEATVSRVLNGRSGVSEATRASVLTALDVLGYERPTKLRGERGRLVGLVLPELQNPIFPALAEVVTASLAQRGFTPALCARTIGGVPERDYIEMLLDHQVSGVVFAGGSYALAEAEHGHYRQLLDRRMPVVMVNGGVDDLGFPHISTDDAVAVEQAFGHLRSLGHEKIGMVMGPEDHVPSRRKLAAVKEVAAWSGPEVDWPVERTNFSMESARLAATRLIQRGNTALICASDVLALGSIRAARRLGLDVPRDVSVVGFDDSVLMTCTDPPLTTVRQPIELMGQAAVDVLVNQIEGAAATTDELLFEPELVVRGSTGPVPHRVAAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7D3</accession>
    <name>U5W7D3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_28000</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560198.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28000"/>
    <dbReference type="EnsemblBacteria" id="AGZ43861">
        <property type="protein sequence ID" value="AGZ43861"/>
        <property type="gene ID" value="AFR_28000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5676"/>
    <dbReference type="eggNOG" id="COG1141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_6_3_11"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001080">
        <property type="entry name" value="3Fe4S_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00352">
        <property type="entry name" value="3FE4SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="74" mass="7523" checksum="E686AF381D128141" modified="2014-01-22" version="1">MTTWRLAVDADTCIGSGMCAGIAPALFTLVDGVAAPVVTQVPPDDAAVDAAESCPVEAITIRDTADDHLVAPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7K4</accession>
    <name>U5W7K4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561329.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44992">
        <property type="protein sequence ID" value="AGZ44992"/>
        <property type="gene ID" value="AFR_33670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6815"/>
    <dbReference type="eggNOG" id="COG2334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965710_0_0_11"/>
    <dbReference type="OrthoDB" id="957729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="34093" checksum="4C48C7995FDD181B" modified="2014-01-22" version="1">MDLPSWTSRWCREHLGAEPVALLHRSEHMSEVLGLRLADGREIAVKARPGQNGREATCVEVQRSLALQGFPCAAPLTGVTVHEGLAVHAEEWRPGGEMRRDDNTGTAAAFAVLLAEQVTLAEDIEVGGTAPGAVEPPLPNPEWVRWDHDRPGLWPPNPYQDRRSGRSLPDGIEDAARRVRERMVAARLPRILGHADWESQNLRWHGDRPWAVHDWDSLAWLPEAAIAGAACGAFASHAQPTLAPIASSEAFLDAYQRARRPFTAEELEVAWAASLWLALHNARGEALWDNPPVAITAVREQGAERLARAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7K8</accession>
    <name>U5W7K8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_28375</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28375"/>
    <dbReference type="EnsemblBacteria" id="AGZ43936">
        <property type="protein sequence ID" value="AGZ43936"/>
        <property type="gene ID" value="AFR_28375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5753"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="16"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="134"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="241"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="393" mass="43320" checksum="497225DD31BE8D4D" modified="2014-01-22" version="1">MFLRVAVRYRRRMRLNEDHRAFRDSVRRFVDAEVNPHVDAWEAAGRVPLHDLFPKAAALGLLGLEYAETYGGEGADHSFQLVAAEEYGRMHATGVGMAIGVQSMMATPSLHEFGSPELKERYLAPAIAGTAVAAVAVTEPDTGSDVARLRTRAVPDGDDLIITGRKTYITNGAQADWICLLARTSDEAGHRGMSQIIVPTSLPGFSVARTFDKLGNRCSDTAELVLDEVRVPAANVVGQVGRGFQQQMQQFVIERMFAAYTTVGGCQYALDRTRKFVLDRTVFGRPLADRQYVAFRLSELQAEVDLLRSHNLVTCETLMAGEDITRMATVAKLKAGRLIREVADWCLQLHGGMGYLEETWTARLYRDTRLTSIGAGADEVMLQILTRLDGLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7R2</accession>
    <name>U5W7R2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561574.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34910"/>
    <dbReference type="EnsemblBacteria" id="AGZ45238">
        <property type="protein sequence ID" value="AGZ45238"/>
        <property type="gene ID" value="AFR_34910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7064"/>
    <dbReference type="eggNOG" id="COG2380">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069565_0_0_11"/>
    <dbReference type="OrthoDB" id="1633344at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="321" mass="33822" checksum="4DF7C967474C2F0C" modified="2014-01-22" version="1">MFVDAWDPSYGASFEGDSSDGPASPSSAQVDTDVEIPSAEWRPLDVAPGVRCPDVVFLVDGVRRNDAGLWTAEEDGMSYAGLAASYAAGVVRCDLGNGIAELVGARVGRGLFTASPSASDVQAGSVRYEVHRVSGTGEASKLPAAVQAPLTALEIDISGAARDGGSDGADLLVVDGPLRNRRQLPRTIGYVKTQQKQYLPASLTPVVTSLRPGQRTPVFHMGSVWGGWSWYLRLPGASGAPWSGIVRVECSPDLAVAEAVELAEVSSATLPRFASSAYKDPRAPQNLVPIAGLERRLRGLLGDSRVLHRALTLATARSRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7V1</accession>
    <name>U5W7V1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561624.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35160"/>
    <dbReference type="EnsemblBacteria" id="AGZ45288">
        <property type="protein sequence ID" value="AGZ45288"/>
        <property type="gene ID" value="AFR_35160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7114"/>
    <dbReference type="eggNOG" id="COG2153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056607_3_1_11"/>
    <dbReference type="OrthoDB" id="1777515at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="15885" checksum="9163DBC6686FBE1B" modified="2014-01-22" version="1">MQPETHVAAFRDLDVSTLYAIMKLRVDVFVVEQKCPYPELDGRDDEPGTRHVWLARGDEILAYLRILDDNGTPRIGRVVTAPDARKGGHASRLMEHALTVIGSRPSTLEAQAHLTGFYSRFGYAEAGPGYLEDGIPHVPMKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W891</accession>
    <name>U5W891_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative malate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_35540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35540"/>
    <dbReference type="EnsemblBacteria" id="AGZ45364">
        <property type="protein sequence ID" value="AGZ45364"/>
        <property type="gene ID" value="AFR_35540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7193"/>
    <dbReference type="eggNOG" id="COG0281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034446_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="malic" evidence="3">
        <location>
            <begin position="94"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Malic_M" evidence="2">
        <location>
            <begin position="239"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="485" mass="51037" checksum="304CDA85DC813602" modified="2014-01-22" version="1">MTTRLPSAGFSITIRIAVTADASSIGRLTTCVGEAGAIVTALDVVDSDPTRVLVDLTCDTADSSHADQVVKQLEAQDGVDVRKVSDRTFLLHLGGKIEVNSKVALRNRDELSRAYTPGVARVCMAIAENPADARRLTIKRNTVAVVSDGSAVLGLGNIGPAAAMPVMEGKAALFKRFGGVDAWPVVLDTQDTDEIVAIVKAIAPAYGGINLEDIAAPRCFEIEMRLRELLDIPVFHDDQHGTAICVLAALTNALRVVGKNLADVRVVVSGAGAAGTAIMKLLLRQGVGDIIAYDRQGALHSGMEDLNASWQWLAEHTNRNNYQGDLAGAIVGADVFIGVSAPNLLTGDDIAKMADKSIVFALANPDPEVDPREARQHAAVVATGRSDQPNQINNVLAFPGVFRGMLDVSAEEFTEEMALAAAMAIANVVGEDKLNPTVIVPSVFDPRVTPAVAAAIRQVVKGGPLPANPAAVPEVELETAPEETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8F8</accession>
    <name>U5W8F8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561765.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35865"/>
    <dbReference type="EnsemblBacteria" id="AGZ45429">
        <property type="protein sequence ID" value="AGZ45429"/>
        <property type="gene ID" value="AFR_35865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7259"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_18_2_11"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="184" mass="20162" checksum="5BC825B2499FEE1F" modified="2014-01-22" version="1">MALGFVRPARPEDAGEIARIQLSTWRTAYRRMFPAHVLANIDEAYLARGWSEAITSPPSPRHRVLIAVEQSESASPVVGFVAAGPADEQALAPEEPALPDDIAAITDLLVEPRWGRRGHGSRLLAATVDLWREDAFKGAVAWSYDADDAMKKFLVSTGWEPDGAARALDVDDMLVPQLRLHVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9W8</accession>
    <name>U5W9W8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562332.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38720"/>
    <dbReference type="EnsemblBacteria" id="AGZ45998">
        <property type="protein sequence ID" value="AGZ45998"/>
        <property type="gene ID" value="AFR_38720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7835"/>
    <dbReference type="eggNOG" id="COG2968">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1287630_0_0_11"/>
    <dbReference type="OrthoDB" id="1821429at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007497">
        <property type="entry name" value="DUF541"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04402">
        <property type="entry name" value="SIMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="23168" checksum="17391ECDBD4A75F2" modified="2014-01-22" version="1">MADQPMIVVRGEATREVPPELAVFSVTVSARDKDRQTALTRLTERAAELRSHLDDFPDAIERRETSGVQVHAELKRGGGERIAAYAGSVSTTVTVTDFSALGELLLRLANQDQTSISGPWWQLRPGSRAGADVRRAAISDALGRAREYAEAVGAEVDRLVEISDQVSDGRGDPMFRMAVGGSSGADLELEVDPQLQTVEATVIVRVTITEPTALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA55</accession>
    <name>U5WA55_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561154.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32790"/>
    <dbReference type="EnsemblBacteria" id="AGZ44816">
        <property type="protein sequence ID" value="AGZ44816"/>
        <property type="gene ID" value="AFR_32790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6636"/>
    <dbReference type="HOGENOM" id="CLU_167427_0_0_11"/>
    <dbReference type="OrthoDB" id="2090945at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="104" mass="11577" checksum="8F0EF5367103A6C9" modified="2014-01-22" version="1">MDILVTVALAVLLVGLFLLALASSSNSRREQLRTANRLTAIEHKLDSIIAHHGITVREREFPDVVRLVQEDKRIEAIRVFRTETGSSLLEAKNAVDAIAARYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAL9</accession>
    <name>U5WAL9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561333.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33690"/>
    <dbReference type="EnsemblBacteria" id="AGZ44996">
        <property type="protein sequence ID" value="AGZ44996"/>
        <property type="gene ID" value="AFR_33690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6819"/>
    <dbReference type="eggNOG" id="COG5635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010524_0_0_11"/>
    <dbReference type="OrthoDB" id="809897at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="322"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1040" mass="115194" checksum="C7F7FAC9D704F445" modified="2014-01-22" version="1">MGSRPLSYADAARLLGVHNNAVIKVLSGVSGGATAALTAGSLADVDFFALRGEVIRWGNEAVTGLRERVTGVRRFDRTLRLEAAHAVVVVTAFYEAVDAVLPDADLTAGEQVAVITSTALRARYADLLQILIETPPPMPAPHRPFETTVADLRVYFRSAAVAVRGFLSGLSAFEDRVPARLEDEVCKIAVRRYIEAYRRLTTEAPEFAVWAAMIEAQATRTLLRNALDDLRSVSGGATVDAVLAGLGKRYRAGLDRAIITSAYAPAHVVLPTLSEGYLDPHGRVAVAGPQDLPATESWWEQAVTVEDVHGFLLAHLTGPDAVTGPLVVLGQPGSGKSAMTRILAARLPPADFLVVRVELRTVPAESSIQSQIEQALLQMLGEHVSWADLTRRAGPALPVVILDGFDELLQATGMNRADYLEEIRRFQSREQELDRPVAVLVTSRTVVADRARIPDSTVVLRLEPFDETQINAWLAVWNAHNAAGLAARDLRPLPPAAALAHGELAVQPLLLLLLALYDAGANALQTAGDDLRRVDLYERLFTDFVVREVGKHSRDLTADQHADEIEVEWRRLGAIAIAILNRGSDVILEPELNDDIPRLLLESDLRRAHDTSAHRALGAGQLMVGRFFFIHESRATRDTAGPERSFEFLHATFGEFLAARRIVSLLVDLAEERLHQRRRRGVIDPGPLYAATSFVTIARRAPLWEMCREMLAHLDPQQRQDCRALALELLADAGYAHPTWTGQDYEPRRTPLAVRHAAFSANLVCFVLLLSDGPVDAIDLVGEPVVTNWRRQALLWQSQLEPEDRKRMWQTLRVAWDLTADPTRLTVRIEDGAAVGVYESVPWPPDTHPWTVRPDDHPHLLAPDVSVRADSRMGRSLRRSAFVQTAHDVREILYDLMPYWEHVGTPRLHRLDDEPVLVSEAALFFELLLAPADAHSPDERARRYAWAFKLIQLPRQRDLLIRQLLEDGPGLTDEGLGQIFHTLGTPDIHRNYETFENLLSAVLERDDGLAVAATLLLNADEDPLEERMAQLYGKLGIEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBA7</accession>
    <name>U5WBA7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ROK-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562825.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41195"/>
    <dbReference type="EnsemblBacteria" id="AGZ46493">
        <property type="protein sequence ID" value="AGZ46493"/>
        <property type="gene ID" value="AFR_41195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8337"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_2_11"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="370" mass="37456" checksum="46536A1A16B5FCBA" modified="2014-01-22" version="1">MTTRATELLRVVHARPGVTRADAARLIGVGTGATTELVGRLGQAELLAEGPTAPSGARGRPTTALIPHPRGPLIAAASITHEAWRVDVVELGGSILATAGEELAGRSGDEVVAALGAAVHRLRIEYRDRIRGIGISVPGTVSRDLMLDASQLGWQDVDLAVAWPRAEIFVAGNDATLAASAESRRGIAVGAAVALHLRIEAGLGGAVVDGGRVLIGALGASGEFGHMPFGDPAVRCPCGAFGCWGTAVDGSALARLLGHETPPDPVTYARRIITDPNPEPAAAAATVATALGRGIAGLVNGLDADLVTLGGLGVDLLAAAPAQLLAAYEAGLMSFRRATPPPVLPALLGDNGPIVGAAEEAWTALLARLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBM2</accession>
    <name>U5WBM2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thiol:disulfide interchange protein dsbE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_41595</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41595"/>
    <dbReference type="EnsemblBacteria" id="AGZ46573">
        <property type="protein sequence ID" value="AGZ46573"/>
        <property type="gene ID" value="AFR_41595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8417"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_11_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666252" evidence="2">
        <location>
            <begin position="18"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="95"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="237" mass="22908" checksum="71CA48F402690307" modified="2014-01-22" version="1">MLLLAACTASKTPPQSATDNTASPFADCAALTSTPSAAARAGSATPGSSAGTGSPAGTGSPAGTGSPGAPGLPGSASDSAGPAVSGSAVAGSSSSAGAADLPAVALPCFTGGESFELTGLRGPAVVNLWASSCAPCREELPLLQELADRTAGQLHVVGVDTFDTRAAGASFAADKGVSFPTLFDPDKKLLTGLGKVNLPVTVFVGADGGRHVYTGKALDKPTLGGLVRTHTGVTVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC94</accession>
    <name>U5WC94_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42625"/>
    <dbReference type="EnsemblBacteria" id="AGZ46779">
        <property type="protein sequence ID" value="AGZ46779"/>
        <property type="gene ID" value="AFR_42625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8626"/>
    <dbReference type="eggNOG" id="ENOG503357V">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048561_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="365" mass="39066" checksum="3F4CC30423291C96" modified="2014-01-22" version="1">MTIMTAESTADQPEGVLLDEPTTADLRAKVTEAWREFAGALAALLPTLSEGAYVDITLDPTASGTGTAVYSVSMRVVADGVIEALAVGNASLPEGFRMNRASVADLVALGWSPPGVLPDSGDSFGLRSSAAQAKQLAAIVSRTLRDVYGAPHPAFLVYLVHDDSDEPIATAPIGTARHEPSIDEELSLDDLAEDGVLSEALANAADEVIPLEERVRTVVATMSKTTIDQLQVDTDGDVGIRAGSAMVFVRVRDNPPLVDVFSPILTEVEPTEQLYVKLSELTNRMPIGRLYCAQDTVWASIPVFGRNFQATHLMLAVQVMTGLADELDDRLHGEFGGKRFFGEGDKPAPKKDDKDGDDHRTGMYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCC0</accession>
    <name>U5WCC0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">zf-TFIIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42750"/>
    <dbReference type="EnsemblBacteria" id="AGZ46804">
        <property type="protein sequence ID" value="AGZ46804"/>
        <property type="gene ID" value="AFR_42750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8652"/>
    <dbReference type="eggNOG" id="COG3809">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147819_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027392">
        <property type="entry name" value="TF_Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13453">
        <property type="entry name" value="zf-TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-TFIIB" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13594" checksum="643F28F65E2E7E2E" modified="2014-01-22" version="1">MRVYERSGVTVDQCTECRGIFLDRGELERLFEAEANFNSRQASSAPRPAQAPQPGGYAAPPPPPPHGGGYASPPPPGYGQPAYPAAPYGAPQPQYGAGHHGHYRQGHHGHYRGRKKKSFLNELFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDV5</accession>
    <name>U5WDV5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562538.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39755"/>
    <dbReference type="EnsemblBacteria" id="AGZ46205">
        <property type="protein sequence ID" value="AGZ46205"/>
        <property type="gene ID" value="AFR_39755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8043"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166206_0_0_11"/>
    <dbReference type="OrthoDB" id="1943389at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="2">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="1">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="10593" checksum="3F0AEE2EE378A790" modified="2014-01-22" version="1">MPSDQNAILADVSAMLRSVLGDFGDDAEVTMTSTFRDDLGLESIDVVSLAGRLQARYGTSVNFAQFVAGLDVESVGELKVGQLVAYIAASLDRRDPVPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEA2</accession>
    <name>U5WEA2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Flagellar biosynthesis protein FlhA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">flhA</name>
        <name evidence="9" type="ORF">AFR_40530</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for formation of the rod structure of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FHIPEP (flagella/HR/invasion proteins export pore) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562692.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40530"/>
    <dbReference type="EnsemblBacteria" id="AGZ46360">
        <property type="protein sequence ID" value="AGZ46360"/>
        <property type="gene ID" value="AFR_40530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8203"/>
    <dbReference type="eggNOG" id="COG1298">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015346_3_0_11"/>
    <dbReference type="OrthoDB" id="436367at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042194">
        <property type="entry name" value="FHIPEP_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042193">
        <property type="entry name" value="FHIPEP_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042196">
        <property type="entry name" value="FHIPEP_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025505">
        <property type="entry name" value="FHIPEP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006301">
        <property type="entry name" value="FlhA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001712">
        <property type="entry name" value="T3SS_FHIPEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30161">
        <property type="entry name" value="PTHR30161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00771">
        <property type="entry name" value="FHIPEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005419">
        <property type="entry name" value="FlhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00949">
        <property type="entry name" value="TYPE3IMAPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01398">
        <property type="entry name" value="FlhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00994">
        <property type="entry name" value="FHIPEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="7" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="680" mass="71635" checksum="2DFA135A268B7FE9" modified="2014-01-22" version="1">MKNRNLSKFAVPIGVIGIIVMMVVPLPTFLLDLLIALNITGALLILLVAMFVQRPLDFSVFPALLLVATLFRLALNISATRLVLRDGDAGKVIHAFGSFVVGGSLVIGLVIFLILIIVQIVVVTKGAERVAEVGARFTLDAMPGKQMAIDADLNAGLIDEATAKKRRADVSAEADFYGAMDGGSKFVKGDAIAAIIITIINLVGGFAIGMLQEGMSPADAMNHYSLLTIGDGLVSQIPALLLSVATGLITTRSATSGDMGSSVTAQLSQNKLAMRIAGGAALGLCVIPGLPKIPFLLIGASVLLIAQRIKDPEPETEGTPAAEIERPSPDSPESLLGEMRVDPLELALSPDLVDLVDTNGGDLLDRVRALRRKMALELGMVMPPVRTRDDLDLPLSSYAIRISGVDAGSGQAPPGTVLAIGDGLQALPGRAGVEPVFGLAGKWVPAELHYQAELSGATVVDRASVIITHLAEIVRQNASRLLGREDVRSLTEMVKRTHPVVVEELTPSLLSLGQIQRVLQALLDEGVPIRDLVRIFESLSLRAKISVDHDGLVEAARAALGPAIAAQYASGGRLTVITLEPMLEQNLLESLRPSETGAFMAIDGMRAEAMVTEAGRLVEAAEQQGITPVLACSPQLRLPLMRLLRAGSRRVQVLSYSEISGSTAQIETMGVVNGAYAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEB4</accession>
    <name>U5WEB4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562707.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40605"/>
    <dbReference type="EnsemblBacteria" id="AGZ46375">
        <property type="protein sequence ID" value="AGZ46375"/>
        <property type="gene ID" value="AFR_40605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8218"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1202737_0_0_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="235" mass="24598" checksum="9CC7948BB807D167" modified="2014-01-22" version="1">MRIPASAPSTLSGGAMQARFLTVISHELRTPLTTIASFTESLDTDDLAPTERSLALAAVRRNTDRMLTLVEDLMVVTRLQTGDLELQPGRVDLGTSIAEAAELLASREPYTAATVQAITGPPLVADAGLLRDLFYAVIGTVASGAADRSASVSTTTGPGGWTVSVTARQAEQLTDEHLMAGMLATPEPPHRRRSTAVWMLIAEAIVTRHGGSVELTFDPDTGAGAVIGLPLNPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF48</accession>
    <name>U5WF48_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-galactoside-specific lectin 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42005"/>
    <dbReference type="EnsemblBacteria" id="AGZ46655">
        <property type="protein sequence ID" value="AGZ46655"/>
        <property type="gene ID" value="AFR_42005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8499"/>
    <dbReference type="eggNOG" id="COG4726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_540411_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="299"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="399"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="582" mass="61146" checksum="E0C15DB29E210D1D" modified="2014-01-22" version="1">MHAGVRTLAARWRRHRHSRRNDTGSMPLALLVTLVGVTLSAGMSGITIGQFKDTQRAADRVAAVNAAQAGLDAGLAQIRSVYKAVGNVPGDLKCLVPTVPVLNAASGGKSDYKISVGYFLTDPAGLVSKLAPIGDLNNITGLLTGGPSAVNNLLNTVTTTVNTVTAATGVTDALQSALGCVNGKLSQVPLYALLRAEGTSGTASRTLYATYTFHTTEDNIPGGRIQLYNNGGPKLCLGAGNAAPKPGDEVVAVACTSSDLRTKFIYPKNLSLVLEGTRAPSVTNAITGVLTGAKTYPYGLCVTATTFTNEAPVKLQECVTPKSPLQQWSYEVNEQTYYATTNGTSSNGYCLNAKTTAVNSPIVVRVNSGTATTNCKSTGNLMHNFSPDAAVGAGSAGINTGQLVNSKEVGRCLDLTNEDVTGAWFTSRKLAPALITYPCKQTFTSSVYWNHKWTAPTIPIGQYKVTGQIYTVPGSGTYAGKLYCLNSPGPAGGYVWVSACSGGGNALQWTLYDQAPKYQDSYQIVDQNGLCLMAAGSLGPAYLYSNTVWSEVITAKCDGGELQKWNVPAYVNPNPLKSMVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFA5</accession>
    <name>U5WFA5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Redox-sensitive transcriptional activator SoxR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_42330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42330"/>
    <dbReference type="EnsemblBacteria" id="AGZ46720">
        <property type="protein sequence ID" value="AGZ46720"/>
        <property type="gene ID" value="AFR_42330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8567"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01110">
        <property type="entry name" value="HTH_SoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010211">
        <property type="entry name" value="Redox-sen_tscrpt-act_SoxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30204:SF0">
        <property type="entry name" value="PTHR30204:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01950">
        <property type="entry name" value="SoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00552">
        <property type="entry name" value="HTH_MERR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="7">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="146" mass="16601" checksum="57EA9FC338667B6A" modified="2014-01-22" version="1">MELLTIGDMAARSGVSPSALRFYEREGLIRSTRTGGNQRRYERHELRRIAFIRIAQQVGVSLEEIRSALATLPESRTPTKADWARLSARWRRKLEDRIAIMERLRDQLTGCIGCGCLSLQRCNLINPEDRLAERGKGPQMILNPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFG4</accession>
    <name>U5WFG4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymethylenebutenolidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562987.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42010"/>
    <dbReference type="EnsemblBacteria" id="AGZ46656">
        <property type="protein sequence ID" value="AGZ46656"/>
        <property type="gene ID" value="AFR_42010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8501"/>
    <dbReference type="eggNOG" id="COG0412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054590_7_3_11"/>
    <dbReference type="OrthoDB" id="1889569at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DLH" evidence="1">
        <location>
            <begin position="16"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="27456" checksum="197A263C83C405E4" modified="2014-01-22" version="1">MRKTMVDVPTEDGVADAYFVRPDGDGPFPGVLFYMDAFGLRPRLFEMADRIAERGYAVLAPNLFYRSSRSPVADLSGLGDAEERGKIFGQLMPMMQLVDTAAITRDTKGYLDFLAAEGQAGGKVAIVGYCMGGTNALKAIEAYPERIVALASFHGARMATEEPDSPHLQVGKITGEVYFAHADHDHAMPQDQIDRLEAALDAADVRFRSEVYAGAEHGFTMSDTAAYSPEGEKRHWEKLFELLDNTLPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VMV4</accession>
    <name>U5VMV4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative cyclopropane fatty acid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFA/CMAS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357247.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00070"/>
    <dbReference type="EnsemblBacteria" id="AGZ38303">
        <property type="protein sequence ID" value="AGZ38303"/>
        <property type="gene ID" value="AFR_00070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.15"/>
    <dbReference type="eggNOG" id="COG2230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026434_6_2_11"/>
    <dbReference type="OrthoDB" id="1518440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003333">
        <property type="entry name" value="Mycolic_cyclopropane_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003085">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="427" mass="46896" checksum="FE68B246F40F5114" modified="2014-01-22" version="1">MPSSTATQLRGLLENLLQSPLPVRLQAWDGSEAGPADAPLLILRNRRALRRLMWQPNELGLARAYVSGDLDVEGDLYEALERLAALIFRGADARGLPMRAVAGDLLKVGVFGLLGPQPKPPPEEIAMRGSRHTRHRDRQAISHHYDVGNDFYRIVLGESMVYSCAYWTLPGDSGYGLADAQRDKLDLICRKLELKPGMRLLDVGCGWGSLALHAAREYGVDVVGITLSTEQADYARKQIAEAGLTAHVEIRIQDYRDLDDGPFDAISSVGMAEHVGTAPYAEYAAILFRQLKAGGRLLNHQIAAIHPAPRTAERSFIDAYVFPDGELAPLGTTVSLLEDAGFEVRDVQTLREHYARTLRAWVTNLESEWSTAVGLTSAGRARVWRLYMAASALAFEHGRIGVNQILAVKDHRDGTSDMPATRAGWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNE8</accession>
    <name>U5VNE8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cysteine desulfuration protein sufE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SufE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00100"/>
    <dbReference type="EnsemblBacteria" id="AGZ38309">
        <property type="protein sequence ID" value="AGZ38309"/>
        <property type="gene ID" value="AFR_00100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.21"/>
    <dbReference type="eggNOG" id="COG2166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124502_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003808">
        <property type="entry name" value="Fe-S_metab-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43597">
        <property type="entry name" value="PTHR43597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02657">
        <property type="entry name" value="SufE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SufE" evidence="2">
        <location>
            <begin position="10"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="15253" checksum="B34A36F514EB6617" modified="2014-01-22" version="1">MPPKLAEIVDEFASAPREVVLEMMLEFSDAVQPLPEALRGHEGMEQVPECQTQFFLRAEVQPDRTVRTYFDCPPEAPTTRAFAGILAEGLEGATADEILAVPDDLYNRMGLATAISPLRIRGGTAILGRLKRQIREQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNN6</accession>
    <name>U5VNN6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RibD_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357486.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01270"/>
    <dbReference type="EnsemblBacteria" id="AGZ38543">
        <property type="protein sequence ID" value="AGZ38543"/>
        <property type="gene ID" value="AFR_01270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.254"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_4_1_11"/>
    <dbReference type="OrthoDB" id="1306955at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="5"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="187" mass="20299" checksum="A170B352346D09B8" modified="2014-01-22" version="1">MPKTQFYTATSIDGFIADEDNSLDWLFKADSDPGSNPFTAFFAEVGAFAMGATTYEWVLEHDQLDKEPVKWADYYGDVPAWVFTHRDLPRVPGADLTFVQGDVREVHAAMTERAAGKNIWLVGGGELAGAFADAGLLDELILGVAPVTLGGGAPLLPRRLEAARLTLTGVEQRGQFAYLTYAVGRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPD7</accession>
    <name>U5VPD7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01915"/>
    <dbReference type="EnsemblBacteria" id="AGZ38672">
        <property type="protein sequence ID" value="AGZ38672"/>
        <property type="gene ID" value="AFR_01915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.388"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_46_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041674">
        <property type="entry name" value="TetR_C_22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17928">
        <property type="entry name" value="TetR_C_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="20555" checksum="AE23B8ED4FE38964" modified="2014-01-22" version="1">MLDACAELVDEVGYEGLTTTLLAERAEVAIGSVYQFFPDKRAIVQALAMRNMDAYLQSLSARFANETFAHWWDGVDAAIDAYIHMHRSVPGFRTLHFGDVVDLHLLDAERDNNAVIGDRLAELLVDQFQLMDRGRLRFALLIAVEASDALIKLAFRRDVTGDEAVLTEAKALIREYLHRHVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR37</accession>
    <name>U5VR37_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF397 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05745"/>
    <dbReference type="EnsemblBacteria" id="AGZ39438">
        <property type="protein sequence ID" value="AGZ39438"/>
        <property type="gene ID" value="AFR_05745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1166"/>
    <dbReference type="HOGENOM" id="CLU_131550_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007278">
        <property type="entry name" value="DUF397"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04149">
        <property type="entry name" value="DUF397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF397" evidence="1">
        <location>
            <begin position="2"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="60" mass="6383" checksum="F8356CB366C7FD97" modified="2014-01-22" version="1">MTWRKSTRSAAAGHCVEVAAAAAAVFVRDSKDVAGPVLEFGAEGWAEFVAGVRDGEFDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRK3</accession>
    <name>U5VRK3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nephrocystin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359025.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06025"/>
    <dbReference type="EnsemblBacteria" id="AGZ39494">
        <property type="protein sequence ID" value="AGZ39494"/>
        <property type="gene ID" value="AFR_06025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1224"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_8_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="806" mass="87319" checksum="273BA31E5FADD8E1" modified="2014-01-22" version="1">MPIRGNLPPRNPRFVGREGLLADVRGLLGNGPVVLLPAPEHQLGGTGRTQLAVEYAYRYADTYDLVWWIPAEQTAGMRAALVGLAQELKLPEARDINRTLGAVRDALSRGEPYRNWLVVFENANRPEDIKPYLPSGAGHVLVTSRNPRWTSEVAQAVPVAVFDRGDSVDFLQRRATDLSASDAERLAERLGDVPMALDLAAAVRAAAGWSITEYLRRYDERAAELAFPTPIRIAWGLAADALGEASPPAFELLELCAFLASAPVSWELLWAARGQVGAELARTVRIERRLKAALRLIGRYGLADLDPPGASVLVHPLVRGMLGQQLSSERHAALLHSVRAMLAAADPGDPDNPAHWSRYAAIVPHLVHSDVLGGDAEEIRQLVINCIRFLYARGDWDSSRDLATQAVARWRDSLGETDEQTLLASFHLANALRAVGRTADARALNAQTLRSQRDVLGDDDETTLATANSVGADLRMQGHFGQARQLDADNLVRYRRLFGESFPTALRCANNLAADLRLLGDFRGARALDEQVLRHRQMIFVEGHPETLSSRAGLAFDLFGLGDYAGATELLSNGHAEALGADHPMALWAGRFAAIAARRCGLPQARQLAETNVELYRQKFGDLNVETLGALVSLANCARDDGDLDEAHRLLERAVARYHAVLGDEHPFTLAAAADLAGVVRATGRFAEARTIDAEALGGLRRSVGADHPFTLSCANGLAADLVATGEAQQAREIAGDTLVRSRALRGAEHPDTLACAFNVALDAGDEPSMHLAVEGLEKVYGEDHRLLARAAEGLRLETEIEPPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS37</accession>
    <name>U5VS37_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06850"/>
    <dbReference type="EnsemblBacteria" id="AGZ39659">
        <property type="protein sequence ID" value="AGZ39659"/>
        <property type="gene ID" value="AFR_06850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1399"/>
    <dbReference type="eggNOG" id="COG3767">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_985645_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665260" evidence="2">
        <location>
            <begin position="32"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="27352" checksum="0EC966A36EBA66C8" modified="2014-01-22" version="1">MNKGRRIGALVAATAASVGMASLVAPTAAQAAPRGPQPVSSGIRAVQAGQDTWVGIRWTTDRRICDVAVRVNGRGVEVDYPGNRRSTSFPRTDSLRPGRTDVTNFRVDADFRRSGIARLSTVIAYDHCGRRDRTIVKRFNLFMPVIVRGGNQGGPGHGHGGPGNGHHNGDGHGHGGPGNGHNNGDGHGHGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGNGGPGGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSB0</accession>
    <name>U5VSB0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359414.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07970"/>
    <dbReference type="EnsemblBacteria" id="AGZ39883">
        <property type="protein sequence ID" value="AGZ39883"/>
        <property type="gene ID" value="AFR_07970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1627"/>
    <dbReference type="eggNOG" id="COG0295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151846_0_0_11"/>
    <dbReference type="OrthoDB" id="2043951at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="123" mass="12047" checksum="C982B379BC33A4A6" modified="2014-01-22" version="1">MPDQTIPAGSVDAELTAEDAKLVTLARGARARVNAAEGASVRDQDGRTYAAANVTLPSMSITALQLAVASAVAAGATKLEAAAVVTEASTLDGAGHAAVRDLAADAPIHVASPTGTLLGSVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSQ4</accession>
    <name>U5VSQ4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)-bd_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359373.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07765"/>
    <dbReference type="EnsemblBacteria" id="AGZ39842">
        <property type="protein sequence ID" value="AGZ39842"/>
        <property type="gene ID" value="AFR_07765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1585"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_5_1_11"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25774" checksum="66C4A45A58F57822" modified="2014-01-22" version="1">MKVAVAGGTGLTGRHVIEVLTAAGHEPVVLARSAGADLMTGAGLDAALDGVDATIDVSNVTTMNRDVAMRFFGTAGRNLLDAAARAGVRHHVALSIVGVDRVKTGYYQGKLHQEQVVREGPVPWTVLRATQFHEFAGQLLERVRGPLAVVPRMTVQPIAVREVAEALVEIVAGEPQKAAPDLAGPQVESLVDMARRLIRAGGSRRRPVVPLWLPGPMASGGLLPTGPGSRGVLTFEQWLGEGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSW2</accession>
    <name>U5VSW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_08350</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359490.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08350"/>
    <dbReference type="EnsemblBacteria" id="AGZ39959">
        <property type="protein sequence ID" value="AGZ39959"/>
        <property type="gene ID" value="AFR_08350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1699"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_5_2_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="29"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="20798" checksum="45284F2592977202" modified="2014-01-22" version="1">MPESVTDGDLIAALGAGGDVARQAWSMLVPRHSPKMWAVARSFAIDAQTAEDLVQTAWLRLLSRADQLRDPQAVGPWLCMIVRNEARRLTTRRREIPAEAPIEHRPAVVDAPDARLLRAERDRAVRLAFSRLGPECRQLLQLLLADPPLSYDEIAAALDRPRGSLGPSRRRCLQRLRDQLPPGYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTN6</accession>
    <name>U5VTN6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative iron-sulfur cluster insertion protein erpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359769.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09365"/>
    <dbReference type="EnsemblBacteria" id="AGZ40162">
        <property type="protein sequence ID" value="AGZ40162"/>
        <property type="gene ID" value="AFR_09365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1909"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_5_0_11"/>
    <dbReference type="OrthoDB" id="1891602at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="FeS_cluster_insertion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00049">
        <property type="entry name" value="TIGR00049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S_biosyn" evidence="1">
        <location>
            <begin position="22"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13303" checksum="4F503C861A75302C" modified="2014-01-22" version="1">MTTESHTESTEATTAAPTGVILTDVAAGNVKRLLEQEGRDDLRLRIAVQPGGCSGLRYQLFFDERSLDGDVVSDFDGVEVVVDRMSSPYLAGATIDFADRIDAQGFTIDNPNAQNSCACGDSFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTT3</accession>
    <name>U5VTT3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative DEAD/DEAH box helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_04105</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04105"/>
    <dbReference type="EnsemblBacteria" id="AGZ39110">
        <property type="protein sequence ID" value="AGZ39110"/>
        <property type="gene ID" value="AFR_04105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.829"/>
    <dbReference type="eggNOG" id="COG0513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_27_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="58"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="260"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="436"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="436"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="604" mass="64007" checksum="75FA7AFDDAB1ABE1" modified="2014-01-22" version="1">MTDIENIESEPALVPVLTRAPVRADSPTFAELGVRAETVEALASAGITRAFAIQEYALPIALRGTDLIGQAPTGTGKTLGFGLPLLERVLAPSEGADGQPQALIVVPTRELGLQVARDIAAAGSTRGVRVLPIYGGVAYETQVETLKKGVEILVGTPGRLLDLAKQKQLKLGSIKALVLDEADRMLDLGFLEDVEKILAMLPSERQTMLFSATMPDPIVALSRRFLNHPVTIHAGHTSDSGPSPLTKQVVYRTHPLNKLEMVARILQARERGLTMIFTRTKRAADRVAEDLDFRGFAVAAVHGDLGQGARERALRAFRSGKIDVLVATDVAARGLDVSGVTHVINYDCPEDPDTYTHRIGRTGRAGATGVAVTFVDWEDMPRWVLIDKSMGLNMPQPPETYHTSTALYSDLDIPSDVSGTLPTAVRTRVGLSAEIEEDLGGTRRRGSRDGGRDGSRDSARGRGRTDRSRGPRRDDAPASPAAEVAAPPADDEAGDRPRRQRRRRRAGDPLTTTDEGTGTAVAPLFADGTEAVTSTDSGPDEGDSEGGSDDTDASGRPRRRRRRGGRGNRGAGGGTEGTDGAEGSDAPGEMSGGSEAGVGTGSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUA0</accession>
    <name>U5VUA0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Activator of Hsp90 ATPase 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360342.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10825"/>
    <dbReference type="EnsemblBacteria" id="AGZ40454">
        <property type="protein sequence ID" value="AGZ40454"/>
        <property type="gene ID" value="AFR_10825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2206"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_0_0_11"/>
    <dbReference type="OrthoDB" id="1498264at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="20542" checksum="1E40B239BBAB9E76" modified="2014-01-22" version="1">MNDIAAQLTAIHRAVSTVKAEGGETVNVLLRRTYNAEVEDVWDALTDPDRVKRWFLPLTGDFREGGTFQLEGNAGGDILTCDPPRLLKVTFGGPVSLVELRLAPDGDESTVLELEHTVPIEMAGSGAGALFVGPGWDGIFVGLGLHLSGEDVKDPVTGDHAHRSIDAWVAAVETSGTASADEIAAGKAAALGQFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUK5</accession>
    <name>U5VUK5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11065"/>
    <dbReference type="EnsemblBacteria" id="AGZ40502">
        <property type="protein sequence ID" value="AGZ40502"/>
        <property type="gene ID" value="AFR_11065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2254"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="24992" checksum="FC3142EC24020918" modified="2014-01-22" version="1">MALHGWFGSAQGWGALPALVDRSRFTYAFPEYRGYGERRGVAGEFSLAEAAGDVLAVADDLGWERFAVVGHSMGASVGQRLLADAPGRVERLAGISPVPATGVPFDEQGWALFSGAAADPGNRRAIIDLTTGNRLAGSWLDDMVSASLATSDTAAFAAYLTAWARTDFAADVTGNPVPALAVIGEHDPAIAEPTMRATWLAHYPNARLEVLANAGHYAMYETPVRLARILDDFLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUN4</accession>
    <name>U5VUN4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclase/dehydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360491.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11215"/>
    <dbReference type="EnsemblBacteria" id="AGZ40532">
        <property type="protein sequence ID" value="AGZ40532"/>
        <property type="gene ID" value="AFR_11215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2284"/>
    <dbReference type="eggNOG" id="COG4276">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112936_0_0_11"/>
    <dbReference type="OrthoDB" id="1927340at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="149" mass="16860" checksum="1F5D751F68221F7A" modified="2014-01-22" version="1">MPTVIEVVTVVDAAPRRVFDLELDVDVHAGSLRGSRETATTSTGRRRLTLGDEATFRATHFGLPWRMTSRITAWEPPHRFVDEQTRGPFRSLHHEHHFEDLGTSATRMTDRMTVSAPLGAFGALVTRLVLAPYLRRLLKQRAAYIRRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUW6</accession>
    <name>U5VUW6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aryl-alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360570.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11440"/>
    <dbReference type="EnsemblBacteria" id="AGZ40577">
        <property type="protein sequence ID" value="AGZ40577"/>
        <property type="gene ID" value="AFR_11440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2332"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_11"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="16"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="37058" checksum="89005B58FD9F7947" modified="2014-01-22" version="1">MEYRTLGRSGCAVSHLCLGTMTFGDESDEEVSHAQLDRFAEAGGTFIDTADVYSAGESERIIGRWYASRSTDVTAPMVLATKGRFPMASDRNGHGLSARHLTQALDASLDRLGLDSVDLYQAHSWDPLTPLEETLRTLDGFVRQGKIRYYGFSNFTGWQLTKAVHLAKSLGLALPVTLQPQYSLLVREIEWEIVPAALDAGLGLLPWSPLGGGWLSGKYRRDQRPTGATRLGEDPNRGMEAWERRGTDRTWQIIDAVQSVAEARGVSMAEVALAWVTDRPGVTSTILGARTLDQLDTNLRASGLHLDPAETAALDEASDLHAPDYPYGALGVDQRSRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUY9</accession>
    <name>U5VUY9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360872.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12635"/>
    <dbReference type="EnsemblBacteria" id="AGZ40813">
        <property type="protein sequence ID" value="AGZ40813"/>
        <property type="gene ID" value="AFR_12635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2566"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_15_5_11"/>
    <dbReference type="OrthoDB" id="1745184at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="21718" checksum="C34E0D83BB27C3D8" modified="2014-01-22" version="1">MRSEKLTRTQLARREDIVRAAVVVVGRDGYAGASIERIAAEANTSKSTVLYHFDTKESVYTAVVDRLYERGRVYMGERLRGEMPVTTRFRAYLDANLRFIAEHAPEVKALHFILENMPRLVSEVDDGLGPLTQMLESGQASGDFAAFDARVAAIAIRSVIDRAAFHMTEHGEVTSQYIEETIHLLERMVGKAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVG9</accession>
    <name>U5VVG9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative fumarylacetoacetate hydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360861.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12580"/>
    <dbReference type="EnsemblBacteria" id="AGZ40802">
        <property type="protein sequence ID" value="AGZ40802"/>
        <property type="gene ID" value="AFR_12580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2555"/>
    <dbReference type="eggNOG" id="COG3970">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078481_0_0_11"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="142"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="30595" checksum="44556DC5C67FD569" modified="2014-01-22" version="1">MLTSLHHLRTGDGSRWAVCQDGKWGDLDGTLAGLLALPLDQARAAVEAAGHQARGDRPGDPTLPPVDRQEVWAAGVTYLRSRDGRKEESGHGSLYDQVYDSDRPELFLKSSPWRVVGTGDAVGIRTDSGWDVPEAEVGLVLNAAGEVFGYTLGNDMSSRSIEGENPLYLPQAKIYDRSCALGPAIVPSWVAGPGPFPIGLRVLRDGHQAYAGTTSTSAMTRCFEDLAGWLFRALSFPAGVVLLTGTGVVPGAEFTTRVGDTIEITSEAFGTLTNTVVAVGDASRGKGQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVI9</accession>
    <name>U5VVI9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39641">
        <property type="protein sequence ID" value="AGZ39641"/>
        <property type="gene ID" value="AFR_06760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1381"/>
    <dbReference type="HOGENOM" id="CLU_1976780_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665762" evidence="2">
        <location>
            <begin position="24"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="12307" checksum="AD73B239550D509B" modified="2014-01-22" version="1">MLLLVLIAAVTTGCSDDTQPPAAAPTPSASVAAQLDDPPGTLTCTKVVSAVREGTLMTPGVVTDIVTASTTADAPVADEAQRLSTAFTAAVAAHNTDNEPDAIAAVSAAAAAMVKVCGDSGLETVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW43</accession>
    <name>U5VW43_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Potassium-transporting ATPase ATP-binding subunit</fullName>
            <ecNumber evidence="13">7.2.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP phosphohydrolase [potassium-transporting] B chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Potassium-binding and translocating subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Potassium-translocating ATPase B chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">kdpB</name>
        <name evidence="15" type="ORF">AFR_13815</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit is responsible for energy coupling to the transport system and for the release of the potassium ions to the cytoplasm.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + K(+)(out) = ADP + H(+) + K(+)(in) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">The system is composed of three essential subunits: KdpA, KdpB and KdpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.6" evidence="13"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361108.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13815"/>
    <dbReference type="EnsemblBacteria" id="AGZ41049">
        <property type="protein sequence ID" value="AGZ41049"/>
        <property type="gene ID" value="AFR_13815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2805"/>
    <dbReference type="eggNOG" id="COG2216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025728_2_0_11"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02078">
        <property type="entry name" value="P-type_ATPase_K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00285">
        <property type="entry name" value="KdpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006391">
        <property type="entry name" value="P-type_ATPase_bsu_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43743">
        <property type="entry name" value="PTHR43743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01497">
        <property type="entry name" value="kdpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 13" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 13" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="275"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="621"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="691"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="13">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="403"/>
            <end position="410"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="555"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="559"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ41049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="719" mass="75159" checksum="0F6D089CADB181A4" modified="2014-01-22" version="1">MTTPTPTLEKPSTDQQPAPSGDRRVGGGLLDPAQLWRSLPAAVAKLDPRTLWHNPVMLIVEAGAVFTTVLAVTDPSVLAWLVTVWLWLTVVFANLAEAVAEGRGKAQAAALRRSKQDMVARRLGKGGAEERVPAPELKLGDTVVVEAGETIPGDGDVIEGIASVDESAITGESAPVIRESGGDRSAVTGGTKVLSDRIVVRITQKPGESFVDRMIALVEGANRQKTPNEIALNILLAALTLIFLLSVVTLQPLAIFSKAFQAAAPDTAAIDGNGVTGIVLVSLIVCLIPTTIGALLSAIGIAGMDRLVQRNVLAMSGRAVEAAGDVNTLLLDKTGTITLGNRQAAEFVPVDGVTAATLADAAQLSSLADETPEGRSVVVLAKTDFGLREREPGLMTHASFVPFTAQTRMSGVDLATDGDQPAREVRKGAASAVMKWVRDNGGHPTEQVGEIVDTVSGTGGTPLVVAERVAGQPARALGVIHLKDVVKSGMSERFAEMRRMGIRTVMITGDNPRTAKAIADEAGVDDFLAEATPEDKLAYIRKEQEGGRLVAMTGDGTNDAPALAQADVGVAMNTGTSAAKEAGNMVDLDSDPTKLIEIVEIGKQLLITRGALTTFSITNDIAKYFAIIPAIFVGVYPGLDALNIMRLSSPGSAILSAVIFNALVIIALIPLALRGVRYRAAGASALLRRNLLVYGLGGVIAPFVGIKLIDLLIQFIPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW59</accession>
    <name>U5VW59_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RDD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359427.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08035"/>
    <dbReference type="EnsemblBacteria" id="AGZ39896">
        <property type="protein sequence ID" value="AGZ39896"/>
        <property type="gene ID" value="AFR_08035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1640"/>
    <dbReference type="eggNOG" id="COG1714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998978_0_0_11"/>
    <dbReference type="OrthoDB" id="1884726at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="6">
        <location>
            <begin position="21"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13450" checksum="C31162C884D87D03" modified="2014-01-22" version="1">MQFAPRPAYLTVGAGGRLGAVLLDGLLIMVTFGIGWLLWMLVTWANGQTPAKQLLGHVVADANTGQAFTWGGMFLREFVIKGLLFGLVNTISFGVFSVVDALFVFRADCRTLHDLVAGSVVRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW76</accession>
    <name>U5VW76_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14015"/>
    <dbReference type="EnsemblBacteria" id="AGZ41089">
        <property type="protein sequence ID" value="AGZ41089"/>
        <property type="gene ID" value="AFR_14015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2843"/>
    <dbReference type="HOGENOM" id="CLU_1648490_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="160" mass="16826" checksum="06BEB9F9624F839F" modified="2014-01-22" version="1">MTVRHTGNGLQLTRSHRSTNPVPVKRSWRGAFTAVVAAVVLCASVTLLLTGVSVVPAAAEGPCPPRASMPAYSGGRELLVRTEPVRGSTALLHLCADPAIGPVRAWEISTARPGTIAVQRVAESVALAAVPLRTDRRMDLVVTVRPGSGQPLTFKFSVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWJ2</accession>
    <name>U5VWJ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359562.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08710"/>
    <dbReference type="EnsemblBacteria" id="AGZ40031">
        <property type="protein sequence ID" value="AGZ40031"/>
        <property type="gene ID" value="AFR_08710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1772"/>
    <dbReference type="eggNOG" id="ENOG5031WTQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187374_0_0_11"/>
    <dbReference type="OrthoDB" id="2094489at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="72" mass="7878" checksum="5CD2BD23DD40C5D9" modified="2014-01-22" version="1">MTEREDGQLCADAEQVAAEEPLTPPADEPAGDIPHRVGCRTELRGWAGAHLHGAVRPRRRAVSRGRPPGRWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWU8</accession>
    <name>U5VWU8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulator of chromosome condensation RCC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14925"/>
    <dbReference type="EnsemblBacteria" id="AGZ41267">
        <property type="protein sequence ID" value="AGZ41267"/>
        <property type="gene ID" value="AFR_14925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3031"/>
    <dbReference type="eggNOG" id="COG5184">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005210_8_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="SSF50985"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005713522" evidence="1">
        <location>
            <begin position="23"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="38557" checksum="875FB2739F587C93" modified="2014-01-22" version="1">MAGTVVMTGAAAASPAASSVSAVSDVAAGYGHTCAVRTDSTLWCWGSNYLGELGDGTTTARTAPVRIGTAATWASIDAGTSYTCAVRTDGSLWCWGNNFRGQLGDGTTTSRTSPVRVGDGTTWASVSAGDSHTCATRTDGSLWCWGFNRFAQLGIGTDPYYATSPVRVGTATTWASVTTGFAHTCAVRTDGTLWCWGDSTDGQLGLGGLSFRDTPAQVGTETTWTGVTAGYAFTCAVRAQGTLWCWGENGYGQLGVSGTHRTAPVQVGTATTWTEVRAGFDTACAVRTDNSLWCWGNNATGQLGDGTTTHRYAPTRIGTGTAWTSDFAVSFHICGFRGDSSLWCWGNNANGQLGDGTSTQRPVPGQVVLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXW0</accession>
    <name>U5VXW0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361903.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17180"/>
    <dbReference type="EnsemblBacteria" id="AGZ41714">
        <property type="protein sequence ID" value="AGZ41714"/>
        <property type="gene ID" value="AFR_17180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17180"/>
    <dbReference type="eggNOG" id="ENOG5030NVK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_890340_0_0_11"/>
    <dbReference type="OrthoDB" id="2192274at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665461" evidence="1">
        <location>
            <begin position="22"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="33116" checksum="A77CA53631BE2395" modified="2014-01-22" version="1">MHVSLTVALILSSAVPSPAAALTLPTFAVGATTSATDVVGSGNTCAGFLDERGHLGPLLGIRRVFANAGQLPPADAVTCHTTQGAKLWYSFKTSCTPTAVAAGSCDTEIQAIAAAMPAGSILTYFHEPEDDMTGVQFVNAFKRVYSQAKAITTTVDVVPVHMAYQWRTGSVNVTNNGTGGDREIQDWIVPVAFSDGYGIDLYWQASTRGSEPDDPVSVGSDPRMLRWYTAFGALGRPLALTEWGIDDDLGVRASRGPAIRASRTWLASHGFRIVCYWQHDNWYLGSSVTPPGGYADPDGVAAYQELVAASIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY17</accession>
    <name>U5VY17_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_17530</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17530"/>
    <dbReference type="EnsemblBacteria" id="AGZ41784">
        <property type="protein sequence ID" value="AGZ41784"/>
        <property type="gene ID" value="AFR_17530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3553"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="5"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="304"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="443" mass="46839" checksum="34A961F41909420A" modified="2014-01-22" version="1">MASRYRLVSRLETGGMAQIWHADDELLDRPVALKLPSGLDPARADVLHLAWKEARMAARLSHPNIAAVHDYNAAMRPEGFMAPFVVMELLAGESLAARLDRSPLSWQESAGIGIAVADALTAAHANGVVHRDIKPGNVMLTPTGVKILDFGISAATGEPDDDETGVTFGTPAYVAPERLDGKPAEPATDVYGLGVLLYEMVTGDPPYPVDTWEELDAARTAPPRSLPAELPVAFRELVELCLREEPDRRPTAAHVSRELAALTPPAAPPAPRKKRRVALFSGLAALVVSLIALFAALGSSRQSIDAAPSTAPSAAPSAAPSGVTPRSAPASTRPAAPPTARATPTPTPTAAAPLRVDDAIDRVRATVRTGRESGQIRPDVAQDLLNLLQPLGRAEAADVSVRVEELQRKVQERTDEGSVDEARADLLSARLADLSRAAERATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY39</accession>
    <name>U5VY39_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360644.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11655"/>
    <dbReference type="EnsemblBacteria" id="AGZ40620">
        <property type="protein sequence ID" value="AGZ40620"/>
        <property type="gene ID" value="AFR_11655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2374"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25887" checksum="EAEAED7D69F34A69" modified="2014-01-22" version="1">MGKLDGKVAVITGATSGMALAGAKLFVDEGAHVFISGRHQDALDEAVKLIGHNVTGVQGDAADLNDLDRLFATVEQEKGSIDVLWASAGVGAQGRLGEITEEHFDAAFSLNARGTLFTVQKALPLFNDGGSIFMTGSNASLRGYPGWSAYAASKAVLPAYARVWVSELRDRKIRVNVLTPGQVATPLMREVMDAQMKAQFESVIPRREMGRPEEIASVALFLASDDSSYVNGMELVADGGSTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY86</accession>
    <name>U5VY86_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_17320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361958.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17320"/>
    <dbReference type="EnsemblBacteria" id="AGZ41742">
        <property type="protein sequence ID" value="AGZ41742"/>
        <property type="gene ID" value="AFR_17320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3512"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="22839" checksum="288484AB75E4569F" modified="2014-01-22" version="1">MITMTGAGKVYPGGVTALAGVSLEVGPGELVGVVGPSGSGKSTMLHLLGALDRPSTGIVRIAGHDVAALPDRALSALRARTIGFVFQQFHLAAGVSAMDNVADGLLYTGVRRRQRRVRAAQALDRVGLRHRAHHRPHELSGGERQRVAIARAVVGEPALLLADEPTGNLDSAAGAVVMRLLHDLHAAGTTVVVITHDRDIAEALPRRVELRDGKLVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYC6</accession>
    <name>U5VYC6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18575</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362366.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18575"/>
    <dbReference type="EnsemblBacteria" id="AGZ41993">
        <property type="protein sequence ID" value="AGZ41993"/>
        <property type="gene ID" value="AFR_18575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3770"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="144"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="23866" checksum="53BB23FD87C0581C" modified="2014-01-22" version="1">MRIILAEDSILLREGLIRLLTEEGHEVLAAVGDGDELIREVEARQPDLIVVDIRMPPTHTDEGLKAALEIRRRWPALGVLVLSQHVEKNYATQLLASDTAGVGYLLKDRVSRVEEFLDSLDRVHNGGAAIDPEVVRQLLARTTHVDPLSKLTNREREVLDQMAQGHTNAAIALKLHISQSAVEKNVNMIFEKLDLTRTTGYSRRVLAVLQYLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYK4</accession>
    <name>U5VYK4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360859.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12570"/>
    <dbReference type="EnsemblBacteria" id="AGZ40800">
        <property type="protein sequence ID" value="AGZ40800"/>
        <property type="gene ID" value="AFR_12570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2553"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="255" mass="27156" checksum="4EF1DDBBE34BC04A" modified="2014-01-22" version="1">MATILITGSSAGIGQAAAAALTESGHEVVLHARNEQRAEEALAAVPRAAGVVVADLASFQQTRDMAAQAEKFGPFDTVVLNAGLGLKHATERQLTTDGNELMFQVNTLSAYLVTGLLSGAQRLIFVSSALAGDGLLDLDDPNFERRPFDGWQAYQDTKLHLILLALGVGRRRPQVQSLAFDPGWVATQMTLRNGDSAPLDAAHAGERLARLATADAVPADYYDTERAWRPGPRENAPENQDGLLALCARLTGVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ01</accession>
    <name>U5VZ01_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362452.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19035"/>
    <dbReference type="EnsemblBacteria" id="AGZ42079">
        <property type="protein sequence ID" value="AGZ42079"/>
        <property type="gene ID" value="AFR_19035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3865"/>
    <dbReference type="eggNOG" id="COG1529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_2_11"/>
    <dbReference type="OrthoDB" id="117507at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="PTHR11908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="Ald_Xan_dh_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="SSF54665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="SSF56003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ald_Xan_dh_C" evidence="1">
        <location>
            <begin position="21"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="738" mass="78319" checksum="4005C1050068D726" modified="2014-01-22" version="1">MNADQIVGRAVPRRDGPAKVSGAARFTAEVPVAGLAHAKLVCSTIAAGEIVGIDLTRARSAPGVVAIMTHDNAPAMRIAPLLITLQGASFTRAPVMQDARIRWNGQPVAIVVAETPEQADHAASLVRISYAPTEARLLFDDLKIGAPQPDHVQGEAPAVTLGDAAAALRTAHTSVDRVYRTPWHTHCAIEPHATTVAWHDERSLTMWDATQAITHSQATLAEVFSLDPQNIRVVSQFVGGAFGNKMMWSHQILCAAAARLAQRPVRLVLSRSDVFRVTGGRTRTEQRVALGARADGTLTALVHEATTTTGVNDGFAEQCTFPARVLYGADAFGIDQRVVELHTPANSSMRAPGESVGSFALECALDELAEQLDLDPLELRTRLEPAVHPTNGKPFSGRHLVEAYRLGAERFGWAERSSTPRSRRDGRWWVGQGVATATYPYTRMPGGRASVTVSADGHATVRTAAHEMGMGTATVQAQYAADHLGLPLDRVHFEYGDSRHPAAVPAGGSAQTVSIIASVVAATRALIAELLRLTASDSPLAGAGTDDVSLRDGGVYLRDGGAGQSYPDILRRAGRGELTGEGAASPPTETAQHAMHSFGVQFCEVRVHDVTGEVRVSRWLGMFDTGRIINPRTAASQFRGAIVMGIGSALSEETVFDERTGRVLNASLAEYHVPVHADIPPIEVAWLGHPDPMAPLGGKGVGEVGMVGVAAAVANAVHNATGRRIRDLPITPDKVLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ19</accession>
    <name>U5VZ19_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Roadblock/LC7 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361034.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13445"/>
    <dbReference type="EnsemblBacteria" id="AGZ40975">
        <property type="protein sequence ID" value="AGZ40975"/>
        <property type="gene ID" value="AFR_13445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2730"/>
    <dbReference type="eggNOG" id="COG2018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094585_2_0_11"/>
    <dbReference type="OrthoDB" id="1723871at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="1">
        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="14865" checksum="988021605E772460" modified="2014-01-22" version="1">MSRSDPARTSQDLVWLLSGLIDRVPDTRSALLLSSDGLRKAAHGLDDDGADHLAAVASGLFSLARSAGTRFGGSDGVRQVVAELDDSLLFVSAAGSGAVLAVIAGKAADPGVLGYEMAQMVKSVRPYLSTPSRHEPDPLGAHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ64</accession>
    <name>U5VZ64_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH:quinone reductase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362526.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19410"/>
    <dbReference type="EnsemblBacteria" id="AGZ42154">
        <property type="protein sequence ID" value="AGZ42154"/>
        <property type="gene ID" value="AFR_19410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3940"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070045_0_0_11"/>
    <dbReference type="OrthoDB" id="1560482at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="33878" checksum="BE18AB741D47D699" modified="2014-01-22" version="1">MSSLSDRYVETTLRRLPARQRSDIERELRASIADAVDDRLEAGADPDEAETAVLTELGDPDRLAAGFADRPLQLIGPRLYLDYLRLLTTLLATVVPAVAAAVGFVRGVEEDSVVTVVGDTLGAAVTTAVHIAVWTTVLFAIIERTPALRRLPGRSWTPDALPAAPPSRRARWGELVAESLAFVLVTAFILLSPVVSTERDAQGDPVGVLSPWFWETGLVWVFVALGAVLLGFSFARYYLRWSLALTLAGALVTVVNAVALVWAAATDRILNPAFVTAAEWPADAARWINIGLIVIALGTLLSAVTEAFGRSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZN0</accession>
    <name>U5VZN0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PMT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_20385</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20385"/>
    <dbReference type="EnsemblBacteria" id="AGZ42349">
        <property type="protein sequence ID" value="AGZ42349"/>
        <property type="gene ID" value="AFR_20385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4133"/>
    <dbReference type="eggNOG" id="COG4346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025132_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="Glyco_trans_39/83"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="PMT" evidence="8">
        <location>
            <begin position="49"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="540" mass="58916" checksum="83A34415AEB1F4A4" modified="2014-01-22" version="1">MTVDLEETPTTDTPPPSRRRPRWLVPLLVLVLLGQMAAAMVVTAVQQTPTIDEPVYVGTAVTYLEQRSLRYNPEHPPLGKLIIAAGLAFADPQLDPAFRGNQSELGRHVLYEAGNDPYELMFLARLPMIILTLLFGLVVFAFARDLTGPAGGLVALALYAFSPDVITHGSLATLDVPAIGFLLTAVWLAWRARSRPYLYLPLAGVALGAALATRMSVLPAVPVLMLLAFLALKRWRGVAAAAGVTVIAFVVVWVVYLAVDPRLTWIEPTGMTPVHGLRGLLVDWLPVPEPYRDGMRIQFAFEDNTFTGFLFGRLYQGGLWYYLPAALLVKTPIGMLLLWLGGTVAMLATPRLRVAALYLVLPAGLLFAVAMTGARDFGTRYAIFMPIFGAVVAAAVVTVRWRWAQITTAVLVLFVAVSSLRTFPYYLPYSNEAFGGPSQTHLRLHDSNVDWGQDLGRLADRLRERYPGERVWLVYKGAGVPSAYGIEAANPLAVPESEVRGLLVVSDSAQAKATGRLKKLIAGSGEPIDDVGHSMTVYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZU6</accession>
    <name>U5VZU6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362880.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21180"/>
    <dbReference type="EnsemblBacteria" id="AGZ42508">
        <property type="protein sequence ID" value="AGZ42508"/>
        <property type="gene ID" value="AFR_21180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4294"/>
    <dbReference type="eggNOG" id="COG3541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095303_0_0_11"/>
    <dbReference type="OrthoDB" id="974296at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018775">
        <property type="entry name" value="RlaP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34817">
        <property type="entry name" value="PTHR34817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10127">
        <property type="entry name" value="RlaP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="26610" checksum="965171176A6B0174" modified="2014-01-22" version="1">MTLDVPAWAAEITVGYPLIFATVSGAHLYGFASVDSDLDLRASHLLPAAEVVGLRSGPETLQSGGWRDGVELDVVSHDLLKFARLLHSRNGYVLEQLLSPLVVVTSPVHSELKALAPGLITSHHAHHYLGFAATQEKLFARTGELKPALYTLRVLLTGIHLMRTGRLETDLGVLGAGLAYVPELIALKRSAEHGPFPAAARAALAQDIPRLRGALEEARDSSALPGQADPAAVEALHELVVRTRLSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZW6</accession>
    <name>U5VZW6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phenylacetic acid degradation protein PaaD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20785"/>
    <dbReference type="EnsemblBacteria" id="AGZ42429">
        <property type="protein sequence ID" value="AGZ42429"/>
        <property type="gene ID" value="AFR_20785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4214"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_11_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011973">
        <property type="entry name" value="PaaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02286">
        <property type="entry name" value="PaaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="10491" checksum="B613685A269324CC" modified="2014-01-22" version="1">MRVTAEMVNGHGTTHGGYVFLLADTAFACACNSHGPVTVAAGADVTFLRPTAEGDQLEAHARERSTSGRSGVYDVTVSRDGDVIAEFRGRSRVISPGRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W059</accession>
    <name>U5W059_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361424.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15415"/>
    <dbReference type="EnsemblBacteria" id="AGZ41365">
        <property type="protein sequence ID" value="AGZ41365"/>
        <property type="gene ID" value="AFR_15415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3130"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_692453_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665939" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="79"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="403" mass="42841" checksum="5939443E909CA26E" modified="2014-01-22" version="1">MTTTKKFRNVTVALGTAVVLVAGAGVADAAVTEKPVTATQQAGWHGITREAVRIDFGQGWVTEGELTYPTRSKGRLPVVVMLHGSGHNDMNQTLPEGGSTFVPLAQAAGREGFATLRFNKRGVTGIGPVESTDPAQLAPKNPYNRIQQDASAAIRFAAKSPHVDPSKIFLLGHSEGTNVAANLASDPKKYGIPKPAGVIEMGVVGLDIKNLLTLQIFGRLLLQMHDEFDVDADGQLTATEAVNGLAAQPKELADQFRTVLLDGKKVLASTDTNHDGQVAIDAEAGPVLRKSTGIDAYPNVPGLDQALVDYLNDIARFPTVSQALPKFSGPTLLLNGENDLQTPARAALVADAAIAKAGNKDHKIVIYPGMAHTMNITPKFTPVFGEPDRQVVTEIQGWLKARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>U5W088</accession>
    <name>U5W088_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Putative multi-sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_21765</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362995.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21765"/>
    <dbReference type="EnsemblBacteria" id="AGZ42623">
        <property type="protein sequence ID" value="AGZ42623"/>
        <property type="gene ID" value="AFR_21765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4410"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_15_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="150"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="216"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="289"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="661"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="HPt" evidence="11">
        <location>
            <begin position="815"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="710"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="4">
        <location>
            <position position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ42623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="912" mass="97523" checksum="437CD5F33E1F6E38" modified="2014-01-22" version="1">MAQQWALIGNIVITCAYAAITVAIVVPVVRAGQLFTNRLAVATAMIFFSCAVGHAFHAAMYWEASWTTATMTGMHQETTTWSLWPSAAWDLFTAAVGVYYWTLRRSYGVLLSGTGSLFVGPQEQQRRHDIELREKVAEGRAQAEAERDAQAAMLRAVITNSQSSIYVKDLQGRYLLANEAFQGAFGVTEAELLGHTDEYLDPVLAPVWRANDRLAHAGAVHVEETSDVDGNTRVYETNKFPLFDAAGELYAICGVSLDVTDLRKARDSAEEARDEALAQSRIKSEFLATMSHEIRTPMNGVLGLASLLIGTELDADQRRYASGIHSAGNALLGVINDILDYSKIEAGKVVLDPDDFDLGALLAETAALVTPAAEDKGLTVVTRRGPDLPATVRGDGGRVRQILLNLAGNAVKFTSKGSVTLRAALVDATGPRVRFEVTDTGIGITVADADRLFEPFTQADASTTRTYGGTGLGLAISRQLTEAMGGVIGVDSEPGQGSTFWCEIPFEPAQGTAGTPCPDATGLRILLVGTGPDHEELEADLRRWHMIVSGAGTGPAAMAALREAAAHGRPYDLLLLDADPGDVDTAGLSRAVAADPSIPAAHVVVLNENAPADPRNSATYLPKPVHRSALFDLLAQSMAAVVPVPVVTRPPAPSKPAGKGTILLVEDNDINQMVAVGVLASLGYRADVAGDGVQALELAGTRRYDAILMDCRMPRMDGFTATAELRRREAAGAARTPIIAMTASALVADRERCLAAGMDDYLAKPVNPAELDATLDRWVHGNPAVPVLPEVVAGHQEDRIGRRLDELAGDRTQPERDLVNRLVESFLKRAPQHVAALAEAYASGDRSTFEDQAHSLKGAAGNIGASAVADLCEQLEDEARAGALSPDTPRTLDRLREELRWTERRLRLVLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Z5</accession>
    <name>U5W0Z5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_23315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363484.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23315"/>
    <dbReference type="EnsemblBacteria" id="AGZ42933">
        <property type="protein sequence ID" value="AGZ42933"/>
        <property type="gene ID" value="AFR_23315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4725"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042257_1_0_11"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="23"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="192"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="41167" checksum="B54D5789E31709E8" modified="2014-01-22" version="1">MNARPRTIVVIAPLRHPIREPHAGGLEATVWDRVRVLRDRGHRVLLCAVEGSQFQEDAPPEFRLPAVVWPDETEATDSTFPPGHLERAVPALEDALDYIRRNADTIDVVDNHSLHPQPLTWAERLGVPVVTTLHTPPLPRLVEAARAAADPGRFLAVSAYTAAEWREHGVGALVMPNAIDGNRWLLGPGGRDLVWFGRIVPEKGLHLAVQAARLTGRVLRIAGRVGDIPYFQQHIRPFLGDGADYLGPLGQPELARLVGGSACALITPLWEEPFGLVVAEAMATGTPVAAFDTGGIGEVAGTASAVRLVPMGDVEALAEAAEQLASDRGRHRQRRRQARAAAVARFSLDRRAAELETVFDLSATTFDLSATTARPGADAWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Z7</accession>
    <name>U5W0Z7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="7">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_22745</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363308.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22745"/>
    <dbReference type="EnsemblBacteria" id="AGZ42819">
        <property type="protein sequence ID" value="AGZ42819"/>
        <property type="gene ID" value="AFR_22745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4610"/>
    <dbReference type="eggNOG" id="COG0303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_1_2_11"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6 7" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="8">
        <location>
            <begin position="171"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="387" mass="38526" checksum="7B5B61B4AE46CEA2" modified="2014-01-22" version="1">MPSIDHSPVPWRTAVKIATGAATPLPPSVVPSSEATGRVLAAPVLARGDLPGFDASAMDGYAVAGRGPWRVLGQVYAGGPVWPAPLGAGEAVEIMTGAVVPAGTVAVLPYETADLNAAGPGPKAHIRRAGEDARAGDELLPAGRLVTPAVAGLLAQAGADSVTVHGRPTVRLVVTGDELVTAGVPGPGQVRDVFTPMVSALVAAAGGVVTDAVLIGDEPELLAAALTTADADVVVVTGSSSAGAADHLHGVLNGIGARRLVDQVACRPGRPQLLAELPGTRWVVGLPGNPFAGLVACVTVLGPLLHGLSGRAAPDLIRLPVTGDLRPAPGVTRLVPVRLAGDHALVVPGGRPASLRGAALADALAVLEDGWTTGIPADLIPLNGLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W135</accession>
    <name>U5W135_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363559.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23490"/>
    <dbReference type="EnsemblBacteria" id="AGZ42968">
        <property type="protein sequence ID" value="AGZ42968"/>
        <property type="gene ID" value="AFR_23490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4760"/>
    <dbReference type="eggNOG" id="COG3222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075662_0_0_11"/>
    <dbReference type="OrthoDB" id="1445278at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018641">
        <property type="entry name" value="Trfase_1_rSAM/selenodom_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36529">
        <property type="entry name" value="PTHR36529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09837">
        <property type="entry name" value="DUF2064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04282">
        <property type="entry name" value="glyco_like_cofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21068" checksum="9C117CE0F9C66335" modified="2014-01-22" version="1">MTVQVLVMAKTPVPGKVKTRLCPPCTPEQAAQVAAAALEDTMNAVDAAYRCLVVEGDHPTPAGWNRMPQRGGTLGERLAHAYADTRIDGMASLLIGMDTPQVTAGLLDAAADELGRADAVLGLAEDGGWWALGLRDPSHGEVLRDVPMSTPDTGRLTLKALQGRGLRVSSLPVLRDVDTYADALEVAALCPAGSRFVAVMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W138</accession>
    <name>U5W138_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16960"/>
    <dbReference type="EnsemblBacteria" id="AGZ41670">
        <property type="protein sequence ID" value="AGZ41670"/>
        <property type="gene ID" value="AFR_16960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3442"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125428_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="72"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="1">
        <location>
            <begin position="72"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="20156" checksum="6591FF496A7B7CE4" modified="2014-01-22" version="1">MPSFQAGAVAELDEGAGAEAGRADELLPLLIAVAPSAADRIRLAERLEGIAPLLLVSDLDELRRLIANAPSRPADDAAPDELLIDTARPAVRHGDREVGLTRLELALLACLRTEPVRAWSYRELHRAVWQDEGLERKADVQSLVKRLRRKLSRIGTGVTIDAVRGVGFRLTDHRQPRVSSDVPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1C1</accession>
    <name>U5W1C1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363293.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22710"/>
    <dbReference type="EnsemblBacteria" id="AGZ42812">
        <property type="protein sequence ID" value="AGZ42812"/>
        <property type="gene ID" value="AFR_22710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4603"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_686539_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="425" mass="47654" checksum="7EA99F541D568A08" modified="2014-01-22" version="1">MSHYFDVSWSWIVEAAGETSWRDVMPLGLAGLFVWVLWLYRAVLSRFARPVVNDFRTTVSVVVPAYREDPEILLECLDSWLSQDPYEVIIVPDVEDTEVLARLAEVDDPRLKVIPFVHRGKRSALGVGIRAATGELVVLADSDTRWEPGLLDAVQMPFVDPEVGGVGTQQNVYQRTTSVWRRIADWLVNLRYYDYVPAMGRAGAVACLSGRTAAYRRSAIVPVLPHLEDEFFLGRRCVAGDDGRLTWLVLASGYKTVHQSSARALSMFPSSFSAFVKQRVRWSRNSYRTYLTALWKGWLWRVPMVTKITVLQILLTPVTMGMALAYLIFSRLELTVHSIVIALVWLLLGRGVRGYSHLRRHPQEILLLPLTSLVVIFIALPIKLYAFFTMNKQGWLTRTADSIGGEGQSAATLTTAEPALAGKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1K2</accession>
    <name>U5W1K2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_23160</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363413.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23160"/>
    <dbReference type="EnsemblBacteria" id="AGZ42902">
        <property type="protein sequence ID" value="AGZ42902"/>
        <property type="gene ID" value="AFR_23160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4695"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034827_0_0_11"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="3">
        <location>
            <begin position="245"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="573" mass="63771" checksum="AE7B8BDA7D07AFB7" modified="2014-01-22" version="1">MADPLLFNPGTYDPAHLDAESRRLLRATVDFFENRGKKVLVDTYNQGIWYADFLDFAAKEGLFATFLTPAADGDGDPGKRWDTARNAALSEILGFYGLGYWYTWQVTVLGLGPVWQSANQAVRAHTAQLLTDGHVMAFGLSERGHGADIYATDLVLTPDGDGGFRANGSKYYIGNGNVAGLVSVFGRRADVEGPDGYVFFAADSRHPAYHLVKNVVNGQMFVSEFRLEDYPVRERDVLHTGRAAFDAALNTVNVGKFNLCTAAIGICEHAMYEAVTHAHRRMLYGKPVTAFPHVRRELTDAYARLVAMKLFSDRAVDYFRSAGPDDRRYLLFNPMTKMKVTTEGEKVVDLLWDVIAAKGFEADTYFDKAAKDIRGLPKLEGTVHVNLALILKFMPNYLFEPAEYAPVPQRHDATDDEFLFRQGPARGLGTIRFHDWRTAYDAHADLPNVARFREQADGLCTLLREHAPDDAQQSDLDFLLALGQLFALVVYGQLILEQSELTGLDRGLLDEIFAILIQDFSAYATELGGKAATTDEQAAWALAHIRRPVWDSGRTEGVWQQVVALSGTYEMQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1R6</accession>
    <name>U5W1R6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363634.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23880"/>
    <dbReference type="EnsemblBacteria" id="AGZ43044">
        <property type="protein sequence ID" value="AGZ43044"/>
        <property type="gene ID" value="AFR_23880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4837"/>
    <dbReference type="eggNOG" id="COG3878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038469_0_0_11"/>
    <dbReference type="OrthoDB" id="1878535at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035948">
        <property type="entry name" value="YwqG-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103032">
        <property type="entry name" value="SSF103032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="37349" checksum="3E7EB5F922F77927" modified="2014-01-22" version="1">MITPPPPPEFLAHLPELTAFARTTTRLHPRPGPVTAAESHIGGPLRWPADEPWPTCTTPVLVREDVPLPDELLERLRAADANREQQPVLTREQNEVRAEIARFVGEGYTGFGSFGDGPVMGHRYAARPHPVPNPMVAVAQLRASDIPDLPRPDGADLLQVLWCPGDHATQPAGPVLHLRWRHEAGISAPSAVPPVGEIGREALVPRPCRLHPEQVVEYPFPEELPQDLLDRVDTWDDKWPEATYVDVAMAPGWKVGGYADWSLTGLGETPCPVCAEPADLLLVIDSEEFDSTSRERWRPADDPDPESSAQPTGVVVGRWGALRIFACLRCPGTPYVVDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W249</accession>
    <name>U5W249_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362389.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42016">
        <property type="protein sequence ID" value="AGZ42016"/>
        <property type="gene ID" value="AFR_18700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3796"/>
    <dbReference type="eggNOG" id="ENOG502ZT6N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307928_0_0_11"/>
    <dbReference type="OrthoDB" id="1844081at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="23766" checksum="E8F59EBA8867ADE8" modified="2014-01-22" version="1">MKPIELAEFQSGVSWWRTETTWPADLHHADYRVLAEQNPNGKFDPVWWASAVKRLTSWKALRPFSGAEVGRRVALNAEELAEAWRTSCEPYGNSDIEQVTWENVRGFPDLVGRLKPTKTPSAVFTSKFCHFLLPRVFPVVDGVALPSGQGTYESYFNQVKATWEATDDDVRTRLKSELRRYLGSEVDSSFPVVTKIVEVALIGRNHQVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W277</accession>
    <name>U5W277_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transposase, IS605 OrfB family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AFR_25360</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25360"/>
    <dbReference type="EnsemblBacteria" id="AGZ43338">
        <property type="protein sequence ID" value="AGZ43338"/>
        <property type="gene ID" value="AFR_25360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5139"/>
    <dbReference type="eggNOG" id="COG0675">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_0_11"/>
    <dbReference type="OrthoDB" id="222391at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="HTH_OrfB_IS605" evidence="13">
        <location>
            <begin position="11"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_IS605" evidence="11">
        <location>
            <begin position="183"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="12">
        <location>
            <begin position="306"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ43338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="418" mass="46604" checksum="8B3208499B8B7B99" modified="2014-01-22" version="1">MNQPGTVLLVQLRYNFRLYPDTAQRKALARSFGCARVVFNDGLRLRQTARENGGKYVSDGELSRRLITEAKATPERAWLGEVSSVVLQQALADLNVAYRNFFASITGKRKGRKVAPPRYRSRKENRQAIRFTRNSRFKVLDNGRLRLPKIGDLAVRWSRTLPSDPSSVAVIKDAAGRYFASFVVKSTDEALPPVESEVGIDLGLTHFAVLSGGTKVAAPKFLRRAARKLKRLQQALSRKQRGSQNRRKAVVKVARAHARVADTRRDWQHQLSTTLIRENQAVYVEDLCVVGLGRTRLAKSVHDAGWAGFTAMLEYKAARYGRTFGRVDRFFPSTRKCSDCGRINDKMALDVRAWDCPCGSHHDRDVNAARNIKAAGQADFNDRGAQVRPAAMLAPRGEAVTHPDAAYSTRSVEGISVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W290</accession>
    <name>U5W290_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363971.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25445"/>
    <dbReference type="EnsemblBacteria" id="AGZ43353">
        <property type="protein sequence ID" value="AGZ43353"/>
        <property type="gene ID" value="AFR_25445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5155"/>
    <dbReference type="eggNOG" id="ENOG502Z92A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_421438_0_0_11"/>
    <dbReference type="OrthoDB" id="1143444at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="665" mass="75253" checksum="94BFC751B8077977" modified="2014-01-22" version="1">MTAPKAGTVNSADGSATVGVQADTVHGGVTIYQVAETDPPERKFLVGVRYLRAGVPSEARKLISRAVMEGHANTKSYFYLMLALLSGRTLQHLSPEDLSVLRSARVWVQDRPQDRWTDAIHVINRLLEAAPSTARTDLNVIIKEFDDLGSQQREEVLRNLKVFLAGPHQDWTWKRAFETARRDRRAEYRVDRVWKFFQPHPAGPRVLWPRPVRFRKRDWALLSVATLLTATSGYFLSVAAWDASWPAALGTLLIAFGTGTLVAFAGLHRQHLMRRRRVKEGQISEHNAREPAPDRYEFASGVDRQFRTYFRRYVPDGAERAVWLAETRGIRQTLRNEVVEIYRERDIDAKQVGWLIRFLVSDVKRQWQTGGLYSFRNQLRIPMATRLAFYLGVGVLGAAVSRLVWDALQTRPLPAVLATILLVTGGVPTVRGWSTLVLERRRVAVEKAEANQALTARTEAHQRWAAKLATRPKDQEMAHWLDCDHKALVELGMQHYKLAPHDVIAHAFIEAPPAGPYKRARVPKGPLRYSRYNLLVFILTIDGVRQMAVDLNFETAEFHDRRRINYRYDAVAAVHVTEADDGQKTLELTLVNSHSIKLVVTGPPPRTKELGEDDRTITAVTLDAAGLDNTLHVLEGIAAEGKQWITYERKREQDGLARLIDAVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2D5</accession>
    <name>U5W2D5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HTH-type transcriptional repressor PurR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18935</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362434.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42061">
        <property type="protein sequence ID" value="AGZ42061"/>
        <property type="gene ID" value="AFR_18935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3846"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="330" mass="35662" checksum="A84722E1101A077F" modified="2014-01-22" version="1">MRDVAKLAGVSHQTVSRVLNEHPNVRQETRDRVLEAMQSLDYRRNLAARTLVTRQSHTLGIIGFETTLFGPASMLYGIEDAARTAGYLVSIAMVRNLDRRPVLEAVDRLRQHAVDGIIAIAPKPAVTNGLLQAPSGLYCVAVGGAGAADAVPTVRIDNAAGARLATQHLLDLGHRTVHHVAGPADWPEAQERVTGWRDTLNEAGATVPPIVPQSWNAESGYSQGRELARDRSVTAIFCANDRIALGVLRALHEAGRKVPEEVSVVGFDDMPDSGYFLPPLTTVRQDFAELGRRSLTLLLEHMAVPDDAESPAHVVVSPELVVRNSTAAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2G0</accession>
    <name>U5W2G0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Malate synthase</fullName>
            <ecNumber evidence="2 7">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_25795</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the malate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="2 7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364111.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25795"/>
    <dbReference type="EnsemblBacteria" id="AGZ43423">
        <property type="protein sequence ID" value="AGZ43423"/>
        <property type="gene ID" value="AFR_25795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5229"/>
    <dbReference type="eggNOG" id="COG2225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018928_3_0_11"/>
    <dbReference type="OrthoDB" id="322024at2"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="PTHR42902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="Malate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="SSF51645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001363-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="532" mass="59369" checksum="85FD4ABA948CDC13" modified="2014-01-22" version="1">METVTVAPSAYDDILTPEAIAFVVALDREFGARRVQLLERRRRRRTARTTELDFLESTRDIRTDDTWSVAPPADDLSDRRVEITGPPERKMTVNALNSGAKVWMADFEDATAPTWDNVVSGQRNLRDALDGTLDFTAPDGREYRIGTDLPTIMVRPRGWHLPECHVRIGGRAVSASLFDFGLYLFHCGRKQIDRGSGPYFYLPKLESHLEARLWNDVFVYAQNLLGIPRGTIRATVLIETITAAFEMEEILYELREHSAGLNAGRWDYLFSMIKNRPDVVLPERSQITMTAPFMRAYTELLVKTCHQRGAHAIGGMAAVVPSRDPVAAKRALNQVRQDKRREVGDGFDGSWVAHPGLVETCREVFDQWLGFEPHQIVRKRDDVRVTAKELLDIHTSAVTVSEVALRTNVSVALRYVAAWLGGRGAVALGGLMEDAATAEICRTQLWQWISTGTPTDYGVPVTAGLVVRMLREELDVLAETGALDEDSARRFGQARTLLTVLLSERTCPEFLTLPAYLRHLTAGATLQPVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Y3</accession>
    <name>U5W2Y3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4097 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364366.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26455"/>
    <dbReference type="EnsemblBacteria" id="AGZ43553">
        <property type="protein sequence ID" value="AGZ43553"/>
        <property type="gene ID" value="AFR_26455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5363"/>
    <dbReference type="eggNOG" id="COG3595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081211_0_1_11"/>
    <dbReference type="OrthoDB" id="1059710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025164">
        <property type="entry name" value="DUF4097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13349">
        <property type="entry name" value="DUF4097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4097" evidence="1">
        <location>
            <begin position="22"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="27336" checksum="E03297CD49F21D31" modified="2014-01-22" version="1">MFEFDHATPVSVSLRLQRGTADVIAEQRDTVQVDIADADPDTFTVRLDGDTLAVHAPESALWQWRRTPKARVIVRVPEGSSLSVKSETADLRAAGRYNTVYAALQSADAEVQHADGDINLKTESGDLRVGQAGSSVRLGSQSGDLEAGDVTGDVTANTASGDIMIRAAAASVHAETASGDVEVGLTRRGRVRLKTASGDVSVGVAAGTGVWLDVSTASGSTVNNLSMTPSGPPAENQATLELVVRTASGDITIHRVHADAPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W311</accession>
    <name>U5W311_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364425.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26605"/>
    <dbReference type="EnsemblBacteria" id="AGZ43583">
        <property type="protein sequence ID" value="AGZ43583"/>
        <property type="gene ID" value="AFR_26605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5392"/>
    <dbReference type="eggNOG" id="ENOG5032ZIA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145468_1_0_11"/>
    <dbReference type="OrthoDB" id="1889547at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="117" mass="12797" checksum="C48FB33D9FECFA77" modified="2014-01-22" version="1">MEALRLILRYAHLIGFALLLGGAIAQFVSGKLRINPAMLWGSIIQVVTGLALAAPLRGGGDDEPSPVKLGVKLLIAILIFVMVFIPWRRKRENVNRGHFIGIIALTLINAAIATFWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3N7</accession>
    <name>U5W3N7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362908.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21320"/>
    <dbReference type="EnsemblBacteria" id="AGZ42536">
        <property type="protein sequence ID" value="AGZ42536"/>
        <property type="gene ID" value="AFR_21320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4325"/>
    <dbReference type="eggNOG" id="COG3878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038469_0_0_11"/>
    <dbReference type="OrthoDB" id="1878535at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="325" mass="36474" checksum="AB1C4C8E260CA278" modified="2014-01-22" version="1">MTRTTPPRPVDIAQVFPELREHTLVATRLHPRPGAPTVNDSSVGGPLLWPADEPWPMCDASSHDTATLLRPQTVRRRRAILGAAWARTRRGEPLQITDEERAALPDTDASEPHYLARQPIAMVPVAQLYRRDVPGLTTDLLQVLWCPLDHDDLEYCPRILLRRRRAADVTTVLQDPPEPVVVNDDYLPEPCVVHPEQVREYQYADLLPPGLAGRIREWDEDDLYFGALSVASGWKVGGFANWNLSDPRPMDCEECGTAMRLLLRVDSAEWDGEGSWRPVEDADNGEFLSLQSPSTPTAVVIGRGYSLWIFTCPVSDDHPHRTAMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3U8</accession>
    <name>U5W3U8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbon monoxide dehydrogenase subunit g</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27180"/>
    <dbReference type="EnsemblBacteria" id="AGZ43697">
        <property type="protein sequence ID" value="AGZ43697"/>
        <property type="gene ID" value="AFR_27180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5508"/>
    <dbReference type="eggNOG" id="COG3427">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046420_2_0_11"/>
    <dbReference type="OrthoDB" id="1719670at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010419">
        <property type="entry name" value="CO_DH_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38588">
        <property type="entry name" value="PTHR38588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06240">
        <property type="entry name" value="COXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="409" mass="39404" checksum="6CD3C55005BA3BD0" modified="2014-01-22" version="1">MKITNEFTVNTPIDRAWTVLTDLEGIAPCLPGAHLTGVEGDTYTGKVKVKVGPVVSDFTGTARFVEKDDHNRRAVIDAKGRDARSAGNAAAVVTAQLRPDGDRTLVSVDTDLKISGRLAQFGSGMIKEVSGKLLNQFVTNLEAKLAAEQPAPAPAASASAAPAATAAVSAARPATVPVPSAPAATASVSAASAATVPVSAAAATTNTTPATTTPAATTTPAGTTTPATGPAPAAAPASEGVLPGVGEAKLGSTLSGDGGSKAGSNGVGATGGVASGSGGGGAVGGETVSAGRTTSGADQAAASDAGAGSASGRENGAVTLPFSADTGPAQAPVAGAREWIAGDPHAVEASGASTTPVAASGGALGVQDQEPEPLDLLALAGGSVYKRAIPVGIGVVAVAAVIIWFVARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3X3</accession>
    <name>U5W3X3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43722">
        <property type="protein sequence ID" value="AGZ43722"/>
        <property type="gene ID" value="AFR_27305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27305"/>
    <dbReference type="HOGENOM" id="CLU_212714_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="31" mass="3346" checksum="336897B0BF5F86AE" modified="2014-01-22" version="1">MIRLVVSWALVAVLLGYGVIQTAITAAKLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4K2</accession>
    <name>U5W4K2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_29360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560470.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44133">
        <property type="protein sequence ID" value="AGZ44133"/>
        <property type="gene ID" value="AFR_29360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5950"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_43_2_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="20578" checksum="C18A18651D075D3A" modified="2014-01-22" version="1">MVRAGLTPERLVQAGAELADEVGFDKVTVSAVARKFNVKVPSLYAHLRNSHDLKTGIAVLALAELADRSSTALAGRSGKDALIALGNVYRDYAREHPGRYEATGMRLDPQTAAASSGPRNSQMIRAILRGYHLSETDQTHAVRLLGSTFNGYIRLEMGGSFDHSSPDSQQSWTRIMDGLDALLRNWPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4N0</accession>
    <name>U5W4N0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23425"/>
    <dbReference type="EnsemblBacteria" id="AGZ42955">
        <property type="protein sequence ID" value="AGZ42955"/>
        <property type="gene ID" value="AFR_23425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4747"/>
    <dbReference type="eggNOG" id="COG1819">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1280921_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="1">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="21957" checksum="51E0F5B312C6541F" modified="2014-01-22" version="1">MLLSTYGSRGDVEPIVALAVELQLRGAQVTVCAPPDFAERLGAAGVPMVPVYQSASALTTAAPPPSTLPERAAAVIASQFETITAAAKGCDAMVVTGMMPAAAGALSVAEKLGIRAVSVTFQQVTLPSPDRKPLAYPGRPFPPDVTDNRVLWDLDAASIDSLFADALNRPAPTFESLSAALGQALQTATRVRAGAVAGTIRTDGAAVAAEQVMSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Q7</accession>
    <name>U5W4Q7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">L-sorbosone dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23255"/>
    <dbReference type="EnsemblBacteria" id="AGZ42921">
        <property type="protein sequence ID" value="AGZ42921"/>
        <property type="gene ID" value="AFR_23255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4713"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024435_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666076" evidence="1">
        <location>
            <begin position="26"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="454" mass="47414" checksum="BDFC15AAAB53BA11" modified="2014-01-22" version="1">MARAVVAGGLGFALAACSSDQPAAPAPAGSASAAAEPSSSAAVPALVATTVRPLAGTGASPFDQERQVQAPPGWTVTVWARVPSARLLAWTPDDRLLVSRPKSGDVVELVPGQGDSPPTQRTLVAGLNQPHGLAFTDDTLYVAESDQVDAFTYRAGVLSDKRVVIDNLPDAKSPELGGAYAHALKSVAVGKDGTIYVSVGSTGNVSAEDRDADPERASILRAPAEGGEPTTFARGVRNGTGLAVDPDGAVWTAVNNRDNTEYPYDGDYDGDGESDQGEVLQEYVNDHPLEPLARLEQGRDLGWPYCNPDPDLQPGAPDSPLSYTERPFVRDIQKNADGSKLDCAALPPVEQGMGAHSAPLGLSFATEPGLAGEYGPGALVGVHGSWNRTPPRAPEVSFFAWRDGALGPQQTLLTGFQAENGSRWGRPVMAVQGPDRALYVSDDLSGSIYRVTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W516</accession>
    <name>U5W516_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TGc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29840"/>
    <dbReference type="EnsemblBacteria" id="AGZ44229">
        <property type="protein sequence ID" value="AGZ44229"/>
        <property type="gene ID" value="AFR_29840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6044"/>
    <dbReference type="eggNOG" id="COG1305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064712_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="80"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="28228" checksum="B8BDA37328D88F7A" modified="2014-01-22" version="1">MTTISATHAAGLDDLPATGFEICNLVRHLVIQPHDAQKLGIVENRFAENQLRSTDSIVGALLALNPAPLTVAREPAQRVIGTCRHFAVLAGALLRYRGIPAKVRCGFGTYFQAGKGLDHWVTEYRENDRWVRIDPETLGSSLVPNPGDLAEGEFLTGGEAWIAFREGRIDAMQFGVPGTENFGPGEIRGNAIRDLACLNKVEMLPWDEWGRMTDSYQGKTGADYDELMDTIATVCAGNDEDAAANLYAAQDLTVPASLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5A3</accession>
    <name>U5W5A3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FAD-binding dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_24470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363750.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43160">
        <property type="protein sequence ID" value="AGZ43160"/>
        <property type="gene ID" value="AFR_24470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4956"/>
    <dbReference type="eggNOG" id="COG3573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022946_0_0_11"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033765">
        <property type="term" value="F:steroid dehydrogenase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014614">
        <property type="entry name" value="UCP036654"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43260">
        <property type="entry name" value="PTHR43260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036654">
        <property type="entry name" value="UCP036654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="3">
        <location>
            <begin position="4"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="551" mass="58882" checksum="9BE997F184753768" modified="2014-01-22" version="1">MDADVIVVGAGLAGLAATAELAEAGRKVLLLDQEGEQSLGGQAFWSFGGLFLVGSPEQRRMGVRDSVDLAWQDWLGSAGFDREEDHWPRQWAEAYVNFAAGEKRSWLHAMGHRLFPVVGWAERGGGAADGHGNSVPRFHLTWGTGPGLVEPFERRVRAAAERGLVVFGFRHRVDGLVKTGGTVTGVQGRLLAATDVRRGESSSREETGDFSYAAQAVIVTSGGIGGDHDLVRKAWPARLGAPPKRMISGVPAHVDGRMLGITEAAGARIINPDRMWHYTEGLRNWDPIWPGHGIRILPGPSSLWLDATGRRLPAPLFPGFDTLGTLKHLMATGHDYSWFVLTQKIIEKEFALSGSEQNPDLTGKSIRQVLGRARAGATPPVEAFKKNGDDFVVASTLSELVAGMNKLVAPEGGPALDLDQVRRTVEARDREMANPFSKDAQVTAIHGARRYRGDKLIRVATPHRLLDASAGPLIAVRLNILTRKTLGGLETDLSARVLQSTGEPLPGVYAAGEVAGFGGGGMHGYNSLEGTFLGGCLFSGRTAGRAAAAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5J6</accession>
    <name>U5W5J6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Abortive infection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363781.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24625"/>
    <dbReference type="EnsemblBacteria" id="AGZ43191">
        <property type="protein sequence ID" value="AGZ43191"/>
        <property type="gene ID" value="AFR_24625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4989"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_948745_0_0_11"/>
    <dbReference type="OrthoDB" id="1623593at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30685" checksum="AF35459C57C612C1" modified="2014-01-22" version="1">MSVIRGTNGKVPAGWKVAGFLALCGASVTLAAVLVGMFAPPWASQDGQAWPVVALAALFILGSTRLCLRLEGRRSVSFRPRFTQLAVGLAGGVALVGLLAWGLMLAGVLYWQPNRGFSASLMLGGTAYFCCAVLVEELAFRGYALQRLAEGVGPRAAVALLAAAFGGYHLLSLGTNPSVKTGGADLLWTAAGPAIGAVVFGVAALRTGGIALPLGLHLGWNWTQWHFFTFPADDNPVGLWNPLVMPWYTEHPAAFRIGYAVAMTLALLLVLFTTRRSGARPLFPAGPSAVALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5S0</accession>
    <name>U5W5S0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polypeptide N-acetylgalactosaminyltransferase 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25380"/>
    <dbReference type="EnsemblBacteria" id="AGZ43340">
        <property type="protein sequence ID" value="AGZ43340"/>
        <property type="gene ID" value="AFR_25380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5142"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044083_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665573" evidence="1">
        <location>
            <begin position="21"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="230"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="359" mass="37478" checksum="095C1EAC97589321" modified="2014-01-22" version="1">MGTAILGLLLVLGLPAPARAAESNGGVRIMPLGASITDGFNVPGGYRIKLWELLTGAGHKIDFVGSAANGPASLGDHDHEGHSGWRIDQIDANVAGWLRAYAPRTILVHLGTNDVNQNYDLPNAPARLGALIDKIRANAPTVELFISTLAPETDPVLESRIQDFNAAIPGIVAQKGPLTHLVDIHAVLTTADIADGTHPTAAGYDKMGARWFSALQATPSALNAQVVPPVGVTVAVASPLSGRCLDVPGTSTTNGTQLHIYDCLGSTIQRWTRSAAGELRVYGDRCLDINGNGTADGTKAQIWTCNGTTAQRFTFQPDGTIVGAGSGKCLDVTASGTTDGTLVQLYTCNGTAAQRWSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W615</accession>
    <name>U5W615_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560951.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31790"/>
    <dbReference type="EnsemblBacteria" id="AGZ44618">
        <property type="protein sequence ID" value="AGZ44618"/>
        <property type="gene ID" value="AFR_31790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6434"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_6_2_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019952">
        <property type="entry name" value="F420_OxRdatse_Rv1855c_pred"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03560">
        <property type="entry name" value="F420_Rv1855c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="31175" checksum="44097DCEB401513C" modified="2014-01-22" version="1">MKLGLHIADFTWPEGAPRLGPVLADVASAADEAGFDRISVMDHLWQIFGTPENEMLEAYTTLGFLAAHTSRAKLLTLVTGVVYRDPGLLAKAVTTLDVLSGGRAMLGIGAAWNEEESQGLGLLFPPTAERFERLEEALQICLQMFHGDETAFHGKHYQLGRLLNSPLPLSRPHPPILIGGGGEKKTLRFVAKYADSCNLFDSHEVAHKLDVLREHCTAEGRDYDTIEKTAILNLDPGAKGERVGEVIADLTRLAALGITVGHGMVAGVHDLGKIETLRTEVMPAIEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6F2</accession>
    <name>U5W6F2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pup--protein ligase</fullName>
            <ecNumber evidence="2">6.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Proteasome accessory factor A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Pup-conjugating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pafA</name>
        <name evidence="4" type="ORF">AFR_26390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine = ADP + phosphate + N(6)-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine.</text>
            <dbReference type="EC" id="6.3.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein degradation; proteasomal Pup-dependent pathway.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein pupylation.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">The reaction mechanism probably proceeds via the activation of Pup by phosphorylation of its C-terminal glutamate, which is then subject to nucleophilic attack by the substrate lysine, resulting in an isopeptide bond and the release of phosphate as a good leaving group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Pup ligase/Pup deamidase family. Pup-conjugating enzyme subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.19" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364341.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26390"/>
    <dbReference type="EnsemblBacteria" id="AGZ43540">
        <property type="protein sequence ID" value="AGZ43540"/>
        <property type="gene ID" value="AFR_26390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5349"/>
    <dbReference type="eggNOG" id="COG0638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040524_0_1_11"/>
    <dbReference type="OrthoDB" id="537546at2"/>
    <dbReference type="UniPathway" id="UPA00997"/>
    <dbReference type="UniPathway" id="UPA00998"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019787">
        <property type="term" value="F:ubiquitin-like protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070490">
        <property type="term" value="P:protein pupylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02111">
        <property type="entry name" value="Pup_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022279">
        <property type="entry name" value="Pup_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004347">
        <property type="entry name" value="Pup_ligase/deamidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42307">
        <property type="entry name" value="PTHR42307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03136">
        <property type="entry name" value="Pup_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018077">
        <property type="entry name" value="UCP018077"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03686">
        <property type="entry name" value="pupylate_PafA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="active site" description="Proton acceptor" evidence="2 3">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR018077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="452" mass="51905" checksum="11144A3AC57F610E" modified="2014-01-22" version="1">MERRIFGLETEYGVTCTYRGQRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATPECDSVTDLVAHDRAGERILEGLLVDAEKRLHDEGIAGEIYLFKNNTDSAGNSYGCHENYLVSRHGEFGRLADVLIPFLVTRQLICGAGKVLQTPRGAVFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMNEVTTLLKVGSADLVLRMIEAGVVMRDLSLENPIRAIREVSHDITGRRKIRLANNKEVSALEIQQEYLAKATEFVERRGGDPTAKRVVELWGRVLNAIETGDLDPVSREIDWVSKYKLIERYQAKHEMPMSHPRIAQLDLAYHDVRRGRGLYALMEKRKQVDRISNDLEIFEAKETPPQTTRARLRGEFIRHAQEKRRDFTVDWVHLKLNDQAQRTVLCKDPFRAYDERVERLIASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6N0</accession>
    <name>U5W6N0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561205.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33050"/>
    <dbReference type="EnsemblBacteria" id="AGZ44868">
        <property type="protein sequence ID" value="AGZ44868"/>
        <property type="gene ID" value="AFR_33050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6689"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062440_3_2_11"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="58"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="29447" checksum="350C09849D9E5ADB" modified="2014-01-22" version="1">MEPAQLDRTPFADLDSMPPAMLAMLVAALEGMSRHPEIRRVRNTAWEALRPAPGQRLLDAGCGAGDVARALAVAVSPGGEVVALDHSAATLSAARDRHDGGAIRYVTGDVSSLDLPDDSFDGVWCERVLQHVDDADVAIGELIRVTRPGGRVCLIDTDWGSLAFDGVDANLARTIVAHMHGRLTPRQVDMGRTLRRRLVGYGLHDLQATPVTCYFPDPASAAVVLPMVNPDVPQEAWMTPEGLRERWFDQVDQAGDDGEFLAVLTIWVVAATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6P1</accession>
    <name>U5W6P1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561215.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33100"/>
    <dbReference type="EnsemblBacteria" id="AGZ44878">
        <property type="protein sequence ID" value="AGZ44878"/>
        <property type="gene ID" value="AFR_33100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6698"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_11"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="3"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="33453" checksum="A6B0CDDE65068B7D" modified="2014-01-22" version="1">MSEIVLGAMLFGTRIDESASFALLDRFVERGGRWIDTANNYMFWGHPSGFGGQSEEVIGRWLAARPGARERVRISTKLGGQPTDAARGLDSAEGLSAGVIRRASELSRKRLGVDRIDLYWAHVEDRTVPLEETVGAFGALVTEGAVARLGASNHPAWRVEQGRGIAATTGVEGWTALQLRHSYLRPRPGAPLPASAHRVVTDEVLDFCRTTGVALWAYTALMNGAYVLPSRLDEAYEHRGTTRRLAALDAVAAELGTGRHQTVLAWLLGGTPPIHPIVGATTVERLDEAMDALDLGLDPDQRTRLDEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6X3</accession>
    <name>U5W6X3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561290.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33475"/>
    <dbReference type="EnsemblBacteria" id="AGZ44953">
        <property type="protein sequence ID" value="AGZ44953"/>
        <property type="gene ID" value="AFR_33475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6775"/>
    <dbReference type="eggNOG" id="COG1141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_6_2_11"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="73" mass="7964" checksum="C85BBD43FE2709A1" modified="2014-01-22" version="1">MSTERLHVDWTACDGRGLCAELLPELLVEDDWGYPVARSGESEPTVPPHLAGPARRAVDRCPALALRLVRSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Y6</accession>
    <name>U5W6Y6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27150"/>
    <dbReference type="EnsemblBacteria" id="AGZ43691">
        <property type="protein sequence ID" value="AGZ43691"/>
        <property type="gene ID" value="AFR_27150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5502"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_851606_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="254"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="34225" checksum="8E685FD2E39C1604" modified="2014-01-22" version="1">MPVPMVGRTDALDELRGAWERVGTRKKCVPAVITGLPGTGKTTLAAAALQVTTPQVVLSGSARLHSPAPYDWLAAALADLGTRPELPVPPDALAWLAQDPDVPRERYTPDALLRLAVTTVRTLVGGRPAVLVVEDLHALDPASLNLIGELAGADLPALVLVTSRPPDEAVSPLLTARVLARLSGAPGAVRQHLGALTAGQVTQILDHVYGDSCPPADVAEAVWRRTGGNPYRLTELIAMAGGPEALADATLPTYLQDQPELTAREREVIGCLAEGMSNKQIARALGISVRTVTVHVSNLLRKTGAASRTEAALWAVQRDLRATAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Z2</accession>
    <name>U5W6Z2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Non-ribosomal peptide synthase:amino acid adenylation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_32570</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561111.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32570"/>
    <dbReference type="EnsemblBacteria" id="AGZ44772">
        <property type="protein sequence ID" value="AGZ44772"/>
        <property type="gene ID" value="AFR_32570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6591"/>
    <dbReference type="eggNOG" id="COG1020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_2_4_11"/>
    <dbReference type="OrthoDB" id="572620at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensatn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="1037"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="412"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="915"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="412"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="915"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="3">
        <location>
            <position position="1072"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1111" mass="115741" checksum="B1E1F10A7A8E7F5D" modified="2014-01-22" version="1">MRLPLSQAQLGVWHALRLDPASPAFDIGGHAELPGPIEADLLERAVGAMVAEVAAFRVRFDDTTEPVTQVIAPEVRMPLARLDLTGPEAARAWMRAELGVPRDPTRDPLLEVALLRVGDERHWWFVRSHHLVLDGYSVPLVCGRVAHIYTALLQGADPGPSPFAPYRGLLDAETAYRESPDFGADREFWLGRMAGAHDVPTLGGRIGPPLGPFHRSAAELPAAVAETLRGRTWVPAVMAAFAGYLARLTGAGELRLGVPLAGRSGPLLRTPGNAANLLPLRVAVRPGRDPADLMAAVAAELRQVVRHQRYRGEDLARDLGAVGAGPALFGPSVNIRDFDHGLTFGAGETMLHDVTAGPVEDLTLSVRREGSRFGLYLDGNAGAYSRSDVEAHLPRFVDYLTAFTGRRVVPRQRSGEYESADQARARRTGGHRHGPAPAVRPPGLAEAFAQQVRATPDALALHDGDRRWTFAALTGRVDRLAAALRVAGAGPEKVVALMLPRGAEYVIGLLAVARTGAAWTPVDPARVAHIPRPDFILAGDALAGEARVGEVLAGNAWGGEVLAGEGAVVAAPPVSSTGGVPLALAAVPVVSVASEAEPPSTWPVPDPRNLASVIATSGSTGSPRAVAVTHGAIANLLSSHRTGIMTATRLRVGHTAPFTVDAALDPLLWMVAGHELVLLPEETYRDPYALVSAVREARVDLIDVPPSYLRVLLDAGLLDGEHRPGVVVFGGEAAPAELWRRLRVAAGRGLNAYGPTEFTVDALVARVSDTEAPVLGVPVAGARALVLDGALRPVPEGGVGELYLGGPGLARGYAGQPGLTAGRFVADPVGPPGERLFRTGDLVRLMVGGDLVFLGRTDDQLKIRGFRVEPGETEAVLAADPGVLACAVVPKPGPGGTVLVAYVVPAPVAPGPIPGQAPGPAAPGPMPGPAAPGPMPGQTPRPVAAGAVPGPVSPDLAAGPVELELVSGPTAPGAAGSRSGGDELAGLLMVRVRSVLPEHLVPAAIMVVPELPLAADGTLDRRALPAPELRGDRPYRAPGTVTESALCDCFAEVLEVGRVGADDDFFALGGHSLLAGRLQGVIRERLDAGVSLRAIFTHRTARALAGLLDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7B7</accession>
    <name>U5W7B7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetoacetate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_27900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27900"/>
    <dbReference type="EnsemblBacteria" id="AGZ43841">
        <property type="protein sequence ID" value="AGZ43841"/>
        <property type="gene ID" value="AFR_27900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5654"/>
    <dbReference type="eggNOG" id="COG4689">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106229_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010451">
        <property type="entry name" value="Acetoacetate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023375">
        <property type="entry name" value="ADC_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06314">
        <property type="entry name" value="ADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160104">
        <property type="entry name" value="SSF160104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="23787" checksum="97964EF8B3783D54" modified="2014-01-22" version="1">MNYPPEPWDLRGQLFLSVFAVPRDTLPALPDPLAATVRPVTIGRKAIVGVAWVRYEPGGVLTYEELLAAALTRSGRRLRVSITDIWVDSVPSRDGGRALWGIPKDLAELTVHTGPSGRTSGAASTARGPIAAASFSPGIRLPGRWPTPMTLAQAFGDTVKHSPVRGHARLRLGGATWRIEPGGPLRHLAGRRPLLTATLADFRLRFGAQADTTYAPAGAPPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7F5</accession>
    <name>U5W7F5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ROK family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33955"/>
    <dbReference type="EnsemblBacteria" id="AGZ45049">
        <property type="protein sequence ID" value="AGZ45049"/>
        <property type="gene ID" value="AFR_33955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6873"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="370" mass="37682" checksum="57432D430A95C4AE" modified="2014-01-22" version="1">MGVNEVGRALPQTVLREATDRRLLDETIRQGRTTRAELADVTGYSRPTVSEAVRRLVEAGLLDATGLKETGRRGRVGTFYSLGAQAGWVLGLELDQSGVHARTADLSGATLSDLRRRPTPPGDVSALVESVRAAVQAIPAGAGPLRAVGVSIANPVDPVTHQTVPLAGTPFPEGLLSPTSMIGDLVKAPVLVDNDVNLAALAEHRAGRATGEASFAYVYVGAGLGVGLYIGDQLIRGAHGLAGEIGYLPGASAPTIAADLAAGGLGRTDAPSNDVAAALMLLDRGDEAALRVLAEAVARAINSVAAVVDPALVLLGGPVGTHPALLPRVREAISFPGPTRIEYGGLGTDAALHGAVQVAVEHARAAAVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7K1</accession>
    <name>U5W7K1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase (ATP)</fullName>
            <ecNumber evidence="1">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28570</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28570"/>
    <dbReference type="EnsemblBacteria" id="AGZ43975">
        <property type="protein sequence ID" value="AGZ43975"/>
        <property type="gene ID" value="AFR_28570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5792"/>
    <dbReference type="eggNOG" id="COG1793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008325_4_0_11"/>
    <dbReference type="OrthoDB" id="1073940at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014146">
        <property type="entry name" value="LigD_ligase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02779">
        <property type="entry name" value="NHEJ_ligase_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_LIGASE_A3" evidence="4">
        <location>
            <begin position="103"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="389" mass="41758" checksum="D72E0EAC8C3DFFA9" modified="2014-01-22" version="1">MSAGEVIEPMLATLGEPPAGDDVALEFKWDGIRCLAYVDDGVRLFSRNGRDITTGYPELAELVPLAAGHRVVLDGELVALDSAGVASFERLQQRMHLSAPAPALVTAVPVVYEVFDLLFLDGEATTALPYLQRRELLAGLDLAGDAVRVPAHFVNADPAAVLTAARSQGLEGIVAKRLGSTYQSGRRSRDWIKVPFNQTQEIVVIGWKPGGGRRSGTIGSLLTAVTGPDGNLAYAGGVGTGFTQAMLEQLQGMLAPLSRPTPAVAGIPRVDARGARWVEPVIVGEVSFRNWTGDGKMRHPSWRGLRPDKSPARVPRVTPPVETIDGVMQTLGGSWRVEVVRRGSVTSYRIVHGEDVIDWLPDIADVERILTRAGVAMEALQPAQRRTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7N1</accession>
    <name>U5W7N1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561461.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34330"/>
    <dbReference type="EnsemblBacteria" id="AGZ45124">
        <property type="protein sequence ID" value="AGZ45124"/>
        <property type="gene ID" value="AFR_34330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6946"/>
    <dbReference type="eggNOG" id="ENOG5030IXN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062170_0_0_11"/>
    <dbReference type="OrthoDB" id="1667833at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="37059" checksum="E206BB52A281D92E" modified="2014-01-22" version="1">MIDEDLQTLYPEVPLDTASLQRAREQVFATVTPRRRRWQPGLAILATAAAVTAVAFVALRPETTPAAPGAMVEVAQILQTAADAEIRTVDPRIPAGQFRYLSTHALSMTYTDAGSQHFAVREGRKIEVWVPADHHDEWAMRESDTGERTWLVGTEAQARKIGAGSPQRPPTDLRGKCGIWYPQQGKNPCTQSGNWQEPTDTFIEKLPRDPGKLLDRLRRDTQGKGNDPDQEVLVYVADALRSARLPADVRAALYRSLALLPDLVITERTATLDGRKGTAFGITAAGQTREIIIDPRTGDFVGERTRLAQKMDRIAAGTVIESSSITYGISRKAGAPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7R5</accession>
    <name>U5W7R5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_34935</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561579.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34935"/>
    <dbReference type="EnsemblBacteria" id="AGZ45243">
        <property type="protein sequence ID" value="AGZ45243"/>
        <property type="gene ID" value="AFR_34935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7069"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="12"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="36293" checksum="146E3997E9BA4053" modified="2014-01-22" version="1">MTRQPVRSPGRPTLDAVAARAGVGRGTASRVLNGSSQVSPQAKAAVEAAIQELGYVPNRAARSLVTQRTDSVALVVSESQERVFGEPFFAGVLRGINAALLETPLQLWLAMAASPEQRERVEHHLTGQHVDGVLLLSLHDDDPLPAVLRERGMPFVFGGRSARAAESDFLVDVDNAAGARKAVEYLLGNGARRVATVAGPQDMEVGRARLAGYRAAVTGPELVEYGDFSEAGGARAMRALLEREPDLDAVFAASDLMASGALRALRDKGLRVPQDVAVIGFEDAPVARQCEPPLTTIHQPVEEMGRRMAELLVCRIRREPVENSHVLLDTHLVRRASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7S6</accession>
    <name>U5W7S6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">18.5 kDa class I heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561379.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33920"/>
    <dbReference type="EnsemblBacteria" id="AGZ45042">
        <property type="protein sequence ID" value="AGZ45042"/>
        <property type="gene ID" value="AFR_33920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6866"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_9_0_11"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="17"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="128" mass="14403" checksum="BA9A4C2E2944FA83" modified="2014-01-22" version="1">MVLRNDFDRLTTRVFDTTTRGTGARLDAYRDGDSFFIDIDLPGVDPASIDVTVDRKVLTVRAERKRVEREGVKYLVAERPTGPVSRQVFLSDSLDTDRLDARYDNGVLTLSIPVTERAKPRKFEVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Z5</accession>
    <name>U5W7Z5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc-containing alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560427.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29145"/>
    <dbReference type="EnsemblBacteria" id="AGZ44090">
        <property type="protein sequence ID" value="AGZ44090"/>
        <property type="gene ID" value="AFR_29145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5904"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_7_0_11"/>
    <dbReference type="OrthoDB" id="831053at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="10"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="32485" checksum="DBB1F493FE02FF9C" modified="2014-01-22" version="1">MMAAVVTAYGTTPRYLEFPEPQLAGEHEIVVEVLAAGLHPRVRMQAGGSHYTSTGTDLPLVPGIDGVGRDADGRLWYFMSLGGRYGSMAERAVIDKRRSVVLPAHTDPVTMAATLNCVIASSLALTERVTLRPGQNVLVLGATGGTGRMAVQVARRLGAGQVTAVGRDAARLTALPGATSTALTGDPDTIARVGAGTDIVLDFLWGEPAAATMAALIAGRTDPIRPLTWVNLGEMAGPRATLPAGALRSTQLQIIGSGQGSLTGATMAAAIPAVAAAIAEGTYAVEPDPFPLHDVERAWSLPPDSSRRIVLSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W842</accession>
    <name>U5W842_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560477.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29395"/>
    <dbReference type="EnsemblBacteria" id="AGZ44140">
        <property type="protein sequence ID" value="AGZ44140"/>
        <property type="gene ID" value="AFR_29395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5958"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_11"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="25586" checksum="0EDC2B02D6DCCB48" modified="2014-01-22" version="1">MRNSLVTGASRGIGRAIAIALAARGDRVAVHYATGREAAEQTLSLLDGDGHVLIGGDVSEAEQAAAVVDHAVTALGGIDVLVNNAAVAPGEDNRHAVADVSYADWQRAWRRMVDVDLIGAANVTYCFAQHLIGRGAPGAVVNVGSRGAFRGEPDYPAYGAAKAGLHAFGQSMAVALAPHGIAVTSVAPGFVSTDRQAGKLAGAAGPSVLAESPFGRVGTPEEVGAAVAYLSSAEATWASGTILDLNGASHLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8F2</accession>
    <name>U5W8F2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_36160</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36160"/>
    <dbReference type="EnsemblBacteria" id="AGZ45488">
        <property type="protein sequence ID" value="AGZ45488"/>
        <property type="gene ID" value="AFR_36160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36160"/>
    <dbReference type="eggNOG" id="COG1295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923704_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017039">
        <property type="entry name" value="Virul_fac_BrkB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03631">
        <property type="entry name" value="Virul_fac_BrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="243"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="243"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="29920" checksum="4357FA5AF394248A" modified="2014-01-22" version="1">MTFYAGIAVVPVALLAIWITGLIAGPDRVRRLTGQTIATLPDAIGAPQALSALIDAGLHLTPMLALASLLPATLYGEGLRRAFVSLRDPGESMVGWRGRILWLPLLAAAPALLLALFLALPVTSSLWTRGGWYAFAGVVLSFLAAWLVLTPVVIWVYRGVAPGRPEWLATVLVGSFTAANLSGFLHGFVLFCSLPLDLGVPFGGFTEIGGVVAVGLWLYLFHVILLVGFAATQSASTYQRARRLRSLEDDPQVPAPSRESRSERRNDPADPVPTSSSGPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8M0</accession>
    <name>U5W8M0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_30105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560618.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30105"/>
    <dbReference type="EnsemblBacteria" id="AGZ44281">
        <property type="protein sequence ID" value="AGZ44281"/>
        <property type="gene ID" value="AFR_30105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6098"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_11"/>
    <dbReference type="OrthoDB" id="1074175at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="76"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="30851" checksum="C03FE2CB25C10823" modified="2014-01-22" version="1">MTDLAQRADFRIKLFNRICFGVLIAFALVWIVPLAWALDTALKPNAETTRTTWAIADPTVDAFGRMLRDTDILTWFGSSLFIATLSAAGTVLVASLAAFALSRMRFRYRNVVFWVVLAGIMIPGQVLIVPWFRQFDALGLLNTWWAVSLPQIPTAIAVFIFKQFFDGLPKELEEVARTDGASYLRIYGRIIMPLARPAVAAVAIFTFVQSWNDLLWPLLVLSNPDIMTIPVGLATVQGAFGIRYADTMASAILGAIPLVAVFLLFQRHIVEGIAGSGLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W974</accession>
    <name>U5W974_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="9">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">argS2</name>
        <name evidence="9" type="synonym">argS</name>
        <name evidence="13" type="ORF">AFR_37495</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562088.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37495"/>
    <dbReference type="EnsemblBacteria" id="AGZ45753">
        <property type="protein sequence ID" value="AGZ45753"/>
        <property type="gene ID" value="AFR_37495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7584"/>
    <dbReference type="eggNOG" id="COG0018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_0_1_11"/>
    <dbReference type="OrthoDB" id="1146366at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arg_tRNA_synt_N" evidence="12">
        <location>
            <begin position="4"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="DALR_1" evidence="11">
        <location>
            <begin position="430"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ45753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="554" mass="60674" checksum="BE8D58CFF0E6E7B9" modified="2014-01-22" version="1">MTPADLAATVLTAARAVFVTRGLDVSLLPATTTVERPRNPEHGDYASTMAMQLTKKVGVPPRELAAALAEELSSLPGIKSVEIAGPGFLNIRLDAAAAGVLARDVVLAGKEYGRTDRLAGQRINLEFVSANPTGPVHIGGVRWAAVGDALSRLLRVAGADVGTEYYFNDAGSQIDRFARSLYAAAKGEPAPEDGYGGAYIAEIATAVQSTAPDVLKLDEPAALEVFRVEGVKLMFAEIRASLDEFGVHFDTYFNEKDLHDRGELQEALDRLREQGHIFEADGATWLRTTEFGDDKDRVLRKSDGDWTYFAADCAYYLDKRKRGFDRVVIMLGADHHGYIGRMKAMSACFGDDPEKNLEILIGQLVSLVRNGEPLRMSKRAGTVITLEDFVDALGVDATRYALARYSSDSPIDIDIDLWTRAKSDNPVYYVQYVAARTASVARQAAEVGLTRGEPDAFRPELLVHEKENDLLKALGEYPAVVGSAAELREPHRVARYLEDLAGDYHRFYDKCRVTPMGDEEITDLMRARLWLNDATRTVIANGLALLGVSAPERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W980</accession>
    <name>U5W980_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560835.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31205"/>
    <dbReference type="EnsemblBacteria" id="AGZ44501">
        <property type="protein sequence ID" value="AGZ44501"/>
        <property type="gene ID" value="AFR_31205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6316"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_3_11"/>
    <dbReference type="OrthoDB" id="1054546at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="16"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="325" mass="34955" checksum="5E7740D6E34D30FE" modified="2014-01-22" version="1">MENRSFGRMGRTVGVIGLGAWQLGADWGSVSEEDAHATLAAAVDSGVTFIDTADVYGDGRSEQVIGQFIKDRPHLTVATKMGRRVPQEPGNYTLDNFRAWTDRSRTNLGVETLDLVQLHCPPTPVFSTEAVYDALDTLVQEKRIAAYGVSVEKVDEALAAIARPGTASVQIILNALRLKPLEQVLPAAAAAGVGIIARVPLASGLLSGRYDEHTTFGADDHRNYNRHGEAFDVGETFSGVDFETGLEAVRRLKPHVPAGATMAQFALRWILDQSGVTVVIPGARNPEQAQGNASAAALAPLDAEAHAAVRSVYDELIRPQVHDRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W991</accession>
    <name>U5W991_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent DNA helicase RecG</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_36580</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561908.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36580"/>
    <dbReference type="EnsemblBacteria" id="AGZ45572">
        <property type="protein sequence ID" value="AGZ45572"/>
        <property type="gene ID" value="AFR_36580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7400"/>
    <dbReference type="eggNOG" id="COG1200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005122_7_1_11"/>
    <dbReference type="OrthoDB" id="234717at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045562">
        <property type="entry name" value="RecG_dom3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033454">
        <property type="entry name" value="RecG_wedge"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19833">
        <property type="entry name" value="RecG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17191">
        <property type="entry name" value="RecG_wedge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="290"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="485"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="733" mass="79159" checksum="F903561F8DA703F9" modified="2014-01-22" version="1">MTDTTTVTTTATPLEQVLGAAKAKALATHLDLHTAGDLIYHFPRRYDERGEHTDMRKLEVGEQVTLLAQVKSTTVRPMRQRRGNMLEITIGDGSGATLTCTFFNQAWRERELRTGRWGLFAGKVTDFRGKRQLNGPAYQLLKADATEDEAAEEIEEFAGALIPVYPAAAAVPTWTIARYVREVLETFQPPDDPLPGTVRATRNLIGIGKALQEIHRPSSDTNLHLAKYRLKWDEAFAVQLTLVQRKARAAASPGRSRPRSEAGLLAAFDASLPYELTDGQAQVGEEIAADLARAHPMHRLLQGEVGSGKTLVAVRAMLQVVDAGGQAALLAPTEVLATQHHRSIGAQLGALGRRGELDGDPDGTQITLVTGSLGAVARRAALAKVADGTAGIVVGTHALLYEGVDFHDLGLVVVDEQHRFGVEQRDALRAKAAQPPHVLVMTATPIPRTVAMTVYGDLETSTLSQLPRGRSPIASHVVPPERPAFLDRAWKRVREEVKAGHQAYVVCPRIGATEAEEDEEPPGDNEPARRPPLAVMEVAPILEEGPLHGLRIGILHGKLPADEKDAVMRRFAAGDLDVLVATTVIEVGVDVPNSTVMIIMDADRFGVSQLHQLRGRVGRGSAPGICLLVTEAMEGTPARERLDAVASTTDGFRLAELDLEQRREGDVLGASQSGKHSHLRLLSLLRDSKLITDARAEATALVLEDPTLAKHPALLASVAALVDEERAEYLEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9H7</accession>
    <name>U5W9H7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562139.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37750"/>
    <dbReference type="EnsemblBacteria" id="AGZ45804">
        <property type="protein sequence ID" value="AGZ45804"/>
        <property type="gene ID" value="AFR_37750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7634"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081768_0_0_11"/>
    <dbReference type="OrthoDB" id="1339160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="31117" checksum="23BFAA864CC1A8A5" modified="2014-01-22" version="1">MRFGLFGTGPWAHLAHAPALAAHPDVEFVGVWGRDPAKAAALAQEHGAKPYAEVDDLIADVDAIAVALPPDIQAGIALRAARAGKHLLLDKPVAFTPEAANEIVAAAKEHEVAGVVFFTRRFMPQIQEFIEQVRATEGWLEARIDHLGSIFQPGNPFGASVWRKEGGGLWDVGPHALALILPVLGPVAEVTALAGARDMTHVLLGHTSGAISTLTLSVDAPEALEREDAAFFGESGVATVPPVPWLPVEAFGRAVDDLISASKGGPASPLDLTFGTEVVTILAAAAESIRTGTTVKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9P5</accession>
    <name>U5W9P5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA_ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562077.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37440"/>
    <dbReference type="EnsemblBacteria" id="AGZ45742">
        <property type="protein sequence ID" value="AGZ45742"/>
        <property type="gene ID" value="AFR_37440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7573"/>
    <dbReference type="eggNOG" id="COG1423">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080123_0_0_11"/>
    <dbReference type="OrthoDB" id="1614130at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021122">
        <property type="entry name" value="RNA_ligase_dom_REL/Rnl2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09414">
        <property type="entry name" value="RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA_ligase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="23829" checksum="9A6AB72ACC04D857" modified="2014-01-22" version="1">MDGQGGKYPRTFHLPDSPGATSDDRIQQDLSWLDGELVVTEKLDGGNLTFTRDAMYARSLDSGTQPWDRPAKALWAMTAYRIPDDWRVCGESMWARRSIAYTDLPGVFIVFGIWDETDTLLGWDDTVDWAQRLELPVVPVLYRGGSLTEARAAWAGQRDLETSEGFVVRSAGRIPAKEFSHRLLKWVRAGHVRTDAAWRHRDDFPLNEFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAK8</accession>
    <name>U5WAK8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4240 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562493.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46159">
        <property type="protein sequence ID" value="AGZ46159"/>
        <property type="gene ID" value="AFR_39525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7996"/>
    <dbReference type="eggNOG" id="ENOG5032V7N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559676_0_0_11"/>
    <dbReference type="OrthoDB" id="1694516at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025334">
        <property type="entry name" value="DUF4240"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14024">
        <property type="entry name" value="DUF4240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4240" evidence="2">
        <location>
            <begin position="27"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="172" mass="19657" checksum="4FD26E0B3D62EF70" modified="2014-01-22" version="1">MLPTPEHEDRFWSLLEEAWAPQSKKTNKARRKLAERRPGKDVDVEDLEEAWEDVQEHLEAACAELTTEELTDLDRVVERKLYNLDRADLQEVTDGSDDGFLYARGFIVAMGRTYYEAAKAEPRLAVPDAEAEEMCYLFAHLNQNRFGTFPETNSGISRESCSNPTGWPTLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAZ1</accession>
    <name>U5WAZ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MerR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561477.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34410"/>
    <dbReference type="EnsemblBacteria" id="AGZ45140">
        <property type="protein sequence ID" value="AGZ45140"/>
        <property type="gene ID" value="AFR_34410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6962"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_4_3_11"/>
    <dbReference type="OrthoDB" id="1748559at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="14350" checksum="4BE4B225E06D756C" modified="2014-01-22" version="1">MLIGELAERAGTSTRTLRYYEQHGLVEPSRDANGYRRYDEAELRVVHEIRALLAVGFGLDDIRPFVACLRAGNTAGHVCPDSVAVLRRKLSEVETYLDQLADVRDRLRGQLVQAIEEREIRCLKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB33</accession>
    <name>U5WB33_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39740"/>
    <dbReference type="EnsemblBacteria" id="AGZ46202">
        <property type="protein sequence ID" value="AGZ46202"/>
        <property type="gene ID" value="AFR_39740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8040"/>
    <dbReference type="eggNOG" id="ENOG502ZC7T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034272_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665742" evidence="1">
        <location>
            <begin position="19"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="557" mass="59712" checksum="DF5E6832CC62B7A8" modified="2014-01-22" version="1">MLALAVLALAAAVNPAAAAGDDDSYWFQLLAKAQADECFNGIGLPYPAGPPCAEGQPKVNQAYVWGLTRIGSHVWFGTGAQPNCLTSGATLDVLKPTVNDNYVCEYGESQTVKNDPTWPAEIGDQRPPEVWLYDARTQRSVNKSREISARSAADAERLRTTIGLRAAGNLDGVVLLGGPALNDSMNLFAFDNRTRRFLGSATLTAYGNIRTFLQAEGGLYLGVGVGTNGISGGAVLRWTGSRRAPFTFDQVATLPVQAADLTYHDGRIATTSWPAEQPTTVDRLAGVWISPVLADGAPGLTTEDATGWKQVWHARLYETDRVVAATYGGGGVASFGGYLYWGTMHVPMKATKVHQSVYPQTTDEAKVNQVVNTQRGISIWRGKDLGLPTQQIDLLYGETELPTYDPATATWSKVSTGRTPLYGKSGFGYPFNNYTWRMAVAGGRLYVGTMDWSYIAQDLLAQGSGFTTADRSGVGASAIDPAAFGGDLWMFPSASEPAQQVSITGLGNYLNYGIRNMVPDGDDLYVGMANPMNLRTDPTDDVPEGGWELIKLTAPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB57</accession>
    <name>U5WB57_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Beta-glucosidase</fullName>
            <ecNumber evidence="2 6">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_40625</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="2 6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562711.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40625"/>
    <dbReference type="EnsemblBacteria" id="AGZ46379">
        <property type="protein sequence ID" value="AGZ46379"/>
        <property type="gene ID" value="AFR_40625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8222"/>
    <dbReference type="eggNOG" id="COG2723">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001859_1_3_11"/>
    <dbReference type="OrthoDB" id="654705at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102483">
        <property type="term" value="F:scopolin beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017736">
        <property type="entry name" value="Glyco_hydro_1_beta-glucosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="Glyco_hydro_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="PTHR10353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03356">
        <property type="entry name" value="BGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="428"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="475" mass="52224" checksum="F5EBA17E7ACE0069" modified="2014-01-22" version="1">MTATVSVESSDEPLASGVRFPDGFVWGAATASYQIEGAVNEDGRGPSIWDTFCRTPGRVHAGHTGDVACDHYHRYVEDVAMMADLGLASYRYSIAWPRIQPDGTGPVNTRGLDFYDRLTDELIGKGIDPVVTLYHWDLPQSLEDRGGWASRETAEAFAEYAQIVHARLGDRVRTWTTLNEPWCSAYLGYGSGRHAPGVTDPASVFKAVHHLLLGHGLAARALRSAGAQSVSITLNPASVLPLDPENAADVEAARIVDGLNNRIFLDPLLRGQYPDDMREHMARFVDLSFIRDGDEAIINAPIDVLGVNYYTPSYVSAKPGTPSAPDAPGTDGVAWREPVGPITDIGWQIEPAALTRLLDRIHRDYPGTPMMITENGASYGDGPSEDGAIHDTNRIEYLDGHLRACLDAISHGVDLRGYFAWSLMDNFEWAEGYAKRFGLVHVDYTTQQRVPKDSARWYQEVIRRNGLTDQGRIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBF1</accession>
    <name>U5WBF1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41395</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562865.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41395"/>
    <dbReference type="EnsemblBacteria" id="AGZ46533">
        <property type="protein sequence ID" value="AGZ46533"/>
        <property type="gene ID" value="AFR_41395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8377"/>
    <dbReference type="eggNOG" id="COG5495">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055635_0_0_11"/>
    <dbReference type="OrthoDB" id="1194694at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018931">
        <property type="entry name" value="DUF2520"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019665">
        <property type="entry name" value="OxRdtase/DH_put_Rossmann_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037108">
        <property type="entry name" value="TM1727-like_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10728">
        <property type="entry name" value="DUF2520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10727">
        <property type="entry name" value="Rossmann-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rossmann-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2520" evidence="2">
        <location>
            <begin position="146"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="295" mass="29968" checksum="9B76C2624535ABE8" modified="2014-01-22" version="1">MSAVQRAGKRASSLVVGVVGAGRVGAVLGAALEAAGHHVVAAAAVSAASRERARRLLPHAEIRPADEVARAATDLLLLAVPDDHLAGVVSGLAATGSLRDGTVVAHTSGAHGLAVLGDRDGIALHPAMTFTGADADLERLPGIAWGITARDRAFASRLVTDLGGIPEWIAEDARPLYHAALAHGANHLVTLVNEAADLLRTAGVEDPATVLSPLLHAALDNALLLGDAALTGPVSRGDAGTVRKHLDHLAPESVPAYLALARRTADRAIAAGRLRPQDAALLLDVLAHATVGSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCA3</accession>
    <name>U5WCA3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Branched-chain-amino-acid transaminase</fullName>
            <ecNumber evidence="9">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AFR_36855</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + L-leucine = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2-oxoglutarate + L-valine = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561961.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36855"/>
    <dbReference type="EnsemblBacteria" id="AGZ45625">
        <property type="protein sequence ID" value="AGZ45625"/>
        <property type="gene ID" value="AFR_36855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7456"/>
    <dbReference type="eggNOG" id="COG0115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031922_0_2_11"/>
    <dbReference type="OrthoDB" id="1275805at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="PTHR11825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ45625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="365" mass="39432" checksum="13BB877AD1EDD330" modified="2014-01-22" version="1">MSGGDNLDFEIRPHPAPVSDADRATLLANPGFGRIFTDHMVTIRYAEGKGWYEPRVEARAPIPMDPAAAVLHYAQEIFEGLKAYTTPDGGVAMFRPDANAARFGQSAQRMAMPVLPQPTFLESLSQLIKIDKRWIPESDEGSLYLRPFAYASEVFLGVRPAMEYLYLVIASPVGAYFSGGVKPVTVWVTPDYTRAAPGGTGAAKCGGNYAAGLSAQAEAIEHGCDQVVYLDAVERKYVDELGGMNIVFVQDDGSLVTPPLTGTILPGITRDSILKLAQRAGRKVEERPVSFDEWRDGAASGRIREAFACGTAAVITPIGTVRHPDGEFTVADGGSGEVTMALRQELGDIQRGRAEDPFGWVHRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCB9</accession>
    <name>U5WCB9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease 3</fullName>
            <ecNumber evidence="6">3.1.26.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ribonuclease III</fullName>
            <shortName evidence="6">RNase III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnc</name>
        <name evidence="11" type="ORF">AFR_36525</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribonuclease III family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561897.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36525"/>
    <dbReference type="EnsemblBacteria" id="AGZ45561">
        <property type="protein sequence ID" value="AGZ45561"/>
        <property type="gene ID" value="AFR_36525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7389"/>
    <dbReference type="eggNOG" id="COG0571">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000907_1_2_11"/>
    <dbReference type="OrthoDB" id="1890943at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="PTHR11207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02191">
        <property type="entry name" value="RNaseIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="RNase III" evidence="10">
        <location>
            <begin position="12"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="9">
        <location>
            <begin position="168"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="212"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ45561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="27638" checksum="D5AD2D702C6CFFE2" modified="2014-01-22" version="1">MSISDKRRRPSTEPLEEAFGIPFEPGLLERALTHRSYAYENGGLPTNERLEFLGDSVLGVVITSALFHNHPDLPEGQLAKLRASVVNMHALADVARTLGPAGLGPHLLLGKGEETTGGRDKASILADTLEALLGAIYLEHGLDMAGEVIHRLFDPLMAESAGRGAALDWKTSLQELTAALGLGVPDYVIDDAGPDHAKTFTAWVVVAGERYGGSDGRSKKQAEQRAAAAAWRTLTERAEAETPPPAVAGGGHTELPEAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCH7</accession>
    <name>U5WCH7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Endoribonuclease L-PSP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563003.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42090"/>
    <dbReference type="EnsemblBacteria" id="AGZ46672">
        <property type="protein sequence ID" value="AGZ46672"/>
        <property type="gene ID" value="AFR_42090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8517"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_4_2_11"/>
    <dbReference type="OrthoDB" id="1850770at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="PTHR11803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="130" mass="13338" checksum="31D6CAA1FCD274A9" modified="2014-01-22" version="1">MRVTLDSPPPAPFGDRFAHVARLDFGGGSLLVLSGQVAVDDAGAVVAPGEAGAQAERIFAIIGGVLAAHGAGFSDVLHVRTFMTNLDDLPQYAAVRGKIFTGQPPASTTVEVSRLFLPGAVLEVEVTAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCM9</accession>
    <name>U5WCM9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rhomboid family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_42365</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42365"/>
    <dbReference type="EnsemblBacteria" id="AGZ46727">
        <property type="protein sequence ID" value="AGZ46727"/>
        <property type="gene ID" value="AFR_42365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8574"/>
    <dbReference type="eggNOG" id="COG0705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055068_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="SSF144091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Rhomboid" evidence="6">
        <location>
            <begin position="131"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="31081" checksum="A426C757AE4CD348" modified="2014-01-22" version="1">MTAPVCYRHPSKETYVRCTRCERPICPDCMNEASVGHQCPECVADGRRTQRPARTAFGGSAAGRSGTATRVLIGINVVVGLISILSGGSGAVAGGGWGGLLGGSTPLTEWGAVLGSALYGPAGDLAVHGIANGEWYRLFTAMFLHYGVLHLLLNMYALWFLGRDIERVLGPLRFVGLYLLAGLGGNVAAYLFSAPNSMTAGASTAVFGLMAAIFVLLKRLNLSVAPILPVIVINVIFTFTISNISIAGHLGGMVIGGAVAAVLAYAPKDKRNLVQGLGTAAIFVILMALAVYRTLSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD99</accession>
    <name>U5WD99_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutaredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_38360</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562261.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38360"/>
    <dbReference type="EnsemblBacteria" id="AGZ45926">
        <property type="protein sequence ID" value="AGZ45926"/>
        <property type="gene ID" value="AFR_38360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7760"/>
    <dbReference type="eggNOG" id="COG0695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_11_1_11"/>
    <dbReference type="OrthoDB" id="2045232at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097573">
        <property type="term" value="F:glutathione oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011915">
        <property type="entry name" value="GlrX_actino"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02200">
        <property type="entry name" value="GlrX_actino"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="3">
        <location>
            <begin position="3"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="80" mass="8778" checksum="9FB5A162B43D3310" modified="2014-01-22" version="1">MLTMYSTSWCGYCHRLKSQLDREGIQYDVVDIEQDPASADFVMSVNGGNQTVPTLRFDDGSALTNPSIVQVKEHLAALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDC9</accession>
    <name>U5WDC9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562359.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38855"/>
    <dbReference type="EnsemblBacteria" id="AGZ46025">
        <property type="protein sequence ID" value="AGZ46025"/>
        <property type="gene ID" value="AFR_38855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7862"/>
    <dbReference type="eggNOG" id="COG3145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048788_4_1_11"/>
    <dbReference type="OrthoDB" id="1391445at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA dealkylation involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035552">
        <property type="term" value="P:oxidative single-stranded DNA demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032854">
        <property type="entry name" value="ALKBH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212">
        <property type="entry name" value="PTHR31212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="1">
        <location>
            <begin position="103"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="22376" checksum="5CD8D104E5CC682A" modified="2014-01-22" version="1">MLDLMGGASTTLEPLPGRLVRHQLTAGAWVDVLSGWVRGSDPVFETLLTEVDWRAERRAMYDGVVDVPRLLRWYGRGETLPHASLTEARDALSAHYAEELGEGFVTAGMCLYRDGRDSVAWHGDTLGRSAREDTMVAIVSFGSPRNLLLRPRAGGSETLRFPLGHGDLIVMGGSCQRTWEHAIPKTAKPVGPRVSVQFRPINVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDP3</accession>
    <name>U5WDP3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha-E domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562469.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39405"/>
    <dbReference type="EnsemblBacteria" id="AGZ46135">
        <property type="protein sequence ID" value="AGZ46135"/>
        <property type="gene ID" value="AFR_39405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7972"/>
    <dbReference type="eggNOG" id="COG2307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071567_0_0_11"/>
    <dbReference type="OrthoDB" id="1433145at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007296">
        <property type="entry name" value="DUF403"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04168">
        <property type="entry name" value="Alpha-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-E" evidence="1">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="313" mass="34925" checksum="F98B670078C85B90" modified="2014-01-22" version="1">MLSRIAESLYWIGRYVERAEDTARILDVHLQRILADPWTSEDAACRSLLAVMGMPMEDEPFTSARVVGLLGLDERSPSSVVGALAAARENARGARETVSSEMWECLNSTWHGLPNARRRVEQQGVHAFFRWVRERCAVMAGLTDATMSRDEGWLFLVLGRSVERVDMTARLLRTHERAGGSIPSWLTLLRSSGAWETFLRTYRGSLDDRHAAEFLLLDRLFPRSVFAALSTAESCLTDLEPDPGRAGVATDAQRIVGRARTNLEFRGADELIDDLAPVLASLEKTCSQVNEAVSRRYFRQTTAVNWVRGAVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE19</accession>
    <name>U5WE19_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_015625691.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40080"/>
    <dbReference type="EnsemblBacteria" id="AGZ46270">
        <property type="protein sequence ID" value="AGZ46270"/>
        <property type="gene ID" value="AFR_40080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8111"/>
    <dbReference type="eggNOG" id="ENOG5032Y97">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188374_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021465">
        <property type="entry name" value="DUF3117"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11314">
        <property type="entry name" value="DUF3117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="55" mass="5648" checksum="9A89D6841CE97662" modified="2014-01-22" version="1">MAAMKPRTGDGPLEVTKEGRGIVMRVPLEGGGRLVVEMTPDEANALGDALKAIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEI9</accession>
    <name>U5WEI9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative N-acetylglucosamine-6-phosphate deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_41030</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="6">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562792.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41030"/>
    <dbReference type="EnsemblBacteria" id="AGZ46460">
        <property type="protein sequence ID" value="AGZ46460"/>
        <property type="gene ID" value="AFR_41030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8305"/>
    <dbReference type="eggNOG" id="COG1820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032482_1_2_11"/>
    <dbReference type="OrthoDB" id="1026967at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="PTHR11113:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00221">
        <property type="entry name" value="nagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="7">
        <location>
            <begin position="40"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="205"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="294"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR038994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="375" mass="38251" checksum="EA9B449A36EA09AB" modified="2014-01-22" version="1">MTEKISGRVVRPGAVVPGYVEVDGPTILSVSEHGHTGDDIIVPGFVDLHCHGGGGHTFTQGDPVDARAAAEFHQAHGTTTMLASLVSSPYELMRDATAAYAPLVAAGVIAGVHFEGPYLSHARCGAQNPSFLRDPSLEELSALVELGAGAVRMVTIAPELPGALAAVEFLARRGVVAALGHTDATYEETLAGVAAGASVGTHLFNGMRPAHHREPGPIVALLDSPSIVCELVADGIHLHDGMLRFAAHAAGPDRAALITDAMDAAGMPDGEYELGGQAVAVADRVARLVRDGSIAGSTLTMDAAFRQAVGAGLDLATASAMASTTPARALGLSDQVGALEAGLRADLVVLNPDLTVKRVMRSGAWLSPPAASGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF71</accession>
    <name>U5WF71_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41510"/>
    <dbReference type="EnsemblBacteria" id="AGZ46556">
        <property type="protein sequence ID" value="AGZ46556"/>
        <property type="gene ID" value="AFR_41510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8399"/>
    <dbReference type="eggNOG" id="ENOG50331V5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126513_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15414" checksum="219B129E8E403367" modified="2014-01-22" version="1">MAAGILALLCLGGVGVFISFYDEATKIERTAPDAVVDSFLRAYLVNRNDEEAALFTCKSGADLTQIDAFRTDIQSREQRFSIGIRVDWTSLTVREEGGKTTVSTDVRRIIADGSERTTDSWQFAMVDQDGWRVCGATPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFV4</accession>
    <name>U5WFV4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Myosin light chain kinase 2, skeletal/cardiac muscle</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_42835</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42835"/>
    <dbReference type="EnsemblBacteria" id="AGZ46821">
        <property type="protein sequence ID" value="AGZ46821"/>
        <property type="gene ID" value="AFR_42835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8665"/>
    <dbReference type="eggNOG" id="ENOG5031T6W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_865048_0_0_11"/>
    <dbReference type="OrthoDB" id="1785568at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665827" evidence="3">
        <location>
            <begin position="24"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="31768" checksum="0426824BE4EB3F9F" modified="2014-01-22" version="1">MTAVALGGFLLGAALLTTGTANADQLEGGARQVVFAGGGVLGLSCKSTPSVESMTVPADGTVRVVNRTGHGAKLLINGTSKGSIPDDGATEVVFRRGTTAVTLDPNCTIADQATPVLVTATPSLTAQPDPMPAPTDTAAAETPSAPSNTPATSDGSGSSLPDSVSPATQPQRPSTSVAGRPSEVRPQTSRVNSVAAQAATTAAQAMPHGGAAPRVKTKATTLRGTGAAVAPAFSGMPPGDEKTILPGVPSLELTPPETQAAPVVPVVPPTDVVAAEPVAAVRPMPESQPIGLLALIAGVSVAGVAVAAIRAIVSQRASRASVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNE2</accession>
    <name>U5VNE2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA pyrophosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_00075</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00075"/>
    <dbReference type="EnsemblBacteria" id="AGZ38304">
        <property type="protein sequence ID" value="AGZ38304"/>
        <property type="gene ID" value="AFR_00075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.16"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139808_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="12973" checksum="DF3FE48B38D5B883" modified="2014-01-22" version="1">MRDGRVLMVHERSLRDGNREWWTLPGGGIDPGETPEDAVRREVLEEVGLVVKEARYLLDTPYPSGMTSIFAVTVADGEPQLGPHVDGPLPVGLDWVPLPAVHPDGPGVPIPPMIVALPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNY6</accession>
    <name>U5VNY6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01075"/>
    <dbReference type="EnsemblBacteria" id="AGZ38504">
        <property type="protein sequence ID" value="AGZ38504"/>
        <property type="gene ID" value="AFR_01075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.214"/>
    <dbReference type="eggNOG" id="COG0317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1159163_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="28056" checksum="F7E101BB5EB220F1" modified="2014-01-22" version="1">MTIIDQHLLRVMPLHAVTELYGEAGLLDRLSLELDRIPEPHRATIADACAWAAELHAGQRRTREPYLNHVLRVTLRMLCHYRVTDPDVLTAGLLHDAVEDQSWAITELSGHGPPPVAEALAVIAGRYNGRVARLVEAVTMPARPEGADRIRHYTDHLAEVLSGEPWARVIKLSDFTDNGVGIIHSIGPVVARSARKYEAALPLLRDLLDRSDTPLPPDVKAHIRDQLDLARRRFTAILTTSSCPEGRSGSRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP07</accession>
    <name>U5VP07_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative anti-sigma factor antagonist</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01215"/>
    <dbReference type="EnsemblBacteria" id="AGZ38532">
        <property type="protein sequence ID" value="AGZ38532"/>
        <property type="gene ID" value="AFR_01215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.242"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138490_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="11"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="15366" checksum="65CEF53F7CF524F1" modified="2014-01-22" version="1">MPVAGRAEPVERVPVLKIGDILLVSIQIDMEDQVALQLQEDLADRIVATGCHGVIIDISALDIVDSFVGRTLATIASVSRVLDAETVVVGMRPAVAITLVELGLSLPGIQTALNVELGIEMLARDRDFELPLDEDDAGEAVVSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPI2</accession>
    <name>U5VPI2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="1">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="1">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02165"/>
    <dbReference type="EnsemblBacteria" id="AGZ38722">
        <property type="protein sequence ID" value="AGZ38722"/>
        <property type="gene ID" value="AFR_02165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.438"/>
    <dbReference type="eggNOG" id="COG1514">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081251_1_0_11"/>
    <dbReference type="OrthoDB" id="1696971at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:2'-5'-RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="PTHR35561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 1" evidence="1">
        <location>
            <begin position="93"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="1">
        <location>
            <begin position="175"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="23851" checksum="B8FC9FA81B8B878F" modified="2014-01-22" version="1">MRAGAAAWVGGIRRAGQVRAGPAGLYSRGVRLFVAVFPPDDVRWDLRRRLAAAGNGDSRRNGDSRRSAAPEVDPALPQRHKGGMRLTAIDKWHVTLAFLGEVPGEQLPDVESALDQVDVPRGVELRLAGGGEFGRGRSTVLWAAVEGDLRGLRAQIVDRLADAGLPQDGRDFTPHLTVAYTQDSAVSHALEGYRGPSWVLDGITLVRSDPNEGYTRLRTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPZ9</accession>
    <name>U5VPZ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02950"/>
    <dbReference type="EnsemblBacteria" id="AGZ38879">
        <property type="protein sequence ID" value="AGZ38879"/>
        <property type="gene ID" value="AFR_02950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.593"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777627_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="385" mass="39759" checksum="45098F32EB592B2F" modified="2014-01-22" version="1">MSAPDPLRDELRELADVVVPADMYERSLQRSRKIGRREAAIGTTAAVVALALLGSGLWRLPSRGPDQEPPVAVAPVGPSATASLAPSVAPTTTRASGNQPDTPSRPDRPRPPRATTTPKSTALGDLPGQIFYADADDNGRVVRLTGAGNPSTVLSASNSTAGVSPDGSRIAYVTSGSLMVVDTGADDPQQVYAGTSSAEQAPAWSPDGSRLLIEASETGVLDVATGELTPLPQGLEGQHFRWSGDGSTLVYATSDCGLEVAAGTTAQSGTPVPDQTGTAGQAACRPVSVDATGDLVAVQLDKDAASEKRETPADAILDTVTGDIVDLPVTGTIIGAVFDPDGNLLVRSKKGSKTRLWLFAPDFTLLVQAKEPSELKGLDLLAYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQT0</accession>
    <name>U5VQT0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rpsS</name>
        <name evidence="7" type="ORF">AFR_04525</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014440726.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04525"/>
    <dbReference type="EnsemblBacteria" id="AGZ39194">
        <property type="protein sequence ID" value="AGZ39194"/>
        <property type="gene ID" value="AFR_04525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.914"/>
    <dbReference type="eggNOG" id="COG0185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144911_0_1_11"/>
    <dbReference type="OrthoDB" id="1893906at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_S19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="93" mass="10784" checksum="ADEFD9A4BC7200F2" modified="2014-01-22" version="1">MPRSLKKGPFVDDHLIKKVEVQNEKNSKNVIKTWSRRSTIIPDMLGHTIAVHDGRKHVPVFITEAMVGHKLGEFALTRTFKGHEKDDRKSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRN8</accession>
    <name>U5VRN8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dnaJ</name>
        <name evidence="11" type="ORF">AFR_00530</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DnaJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357339.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00530"/>
    <dbReference type="EnsemblBacteria" id="AGZ38395">
        <property type="protein sequence ID" value="AGZ38395"/>
        <property type="gene ID" value="AFR_00530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.108"/>
    <dbReference type="eggNOG" id="COG0484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_6_11"/>
    <dbReference type="OrthoDB" id="1738789at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5 6 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="10">
        <location>
            <begin position="168"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="181"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="198"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="220"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="234"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="7">
        <location>
            <begin position="168"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="82"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ38395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="42290" checksum="AE400F1464A53551" modified="2014-01-22" version="1">MSSKDWLEKDFYAVLGVGKSASADEIKKAYRKLARENHPDHNPGNKDAEDRFKSASEAYDVLSDDKKRKEYDEMRSLFGSGAFRRGARPGGGSQFDPSDLFGGGFSGAGQPGGAGDRRFGGAGFSDIFSSIFSGGGAGGAAAGGRRGPQRGRDVEAEVTLDFAHAVKGTTLPLTLRSPGVCDTCHGNGAKPGTVPRTCPQCHGSGLISRNQGSFSFSEPCRECQGAGSIVDQKCPECRGTGGVTKNRTINVRFPAGVADGQRIRLSGRGEPGEKGGPAGDLYVQVKVRPDDLFGRTGDDITLTVPITFAEAVLGTDLRVPTLDSPVTLRVPPGTPSGRTLRARGKGVQRRDGQAGDLLVTVEVQVPAVAAVSTEARDALEQFAKLTPNAERERIEARLRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS05</accession>
    <name>U5VS05_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Spermidine/putrescine-binding periplasmic protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06650"/>
    <dbReference type="EnsemblBacteria" id="AGZ39619">
        <property type="protein sequence ID" value="AGZ39619"/>
        <property type="gene ID" value="AFR_06650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1357"/>
    <dbReference type="eggNOG" id="COG0687">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_1_2_11"/>
    <dbReference type="OrthoDB" id="1264541at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019808">
        <property type="term" value="F:polyamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015846">
        <property type="term" value="P:polyamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001188">
        <property type="entry name" value="Sperm_putr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00909">
        <property type="entry name" value="SPERMDNBNDNG"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="417" mass="45556" checksum="69B4C77E408B6E00" modified="2014-01-22" version="1">MSQPFSPDPSLIRGMTQRRLGRRDALRLSGLSAFAMALSACGVKGTGTSAPSAPAADAVAKYWAGKTSNGKVDFANWPLYMDPKQPELKKFTAATGVQVKYQEVIQEMGPWFAKVQPQLAAGQSIGYDLMVITNGVQFKQFVDSGFLAPLDHSKLPNYVANADKSYAQEAFDPGNVYTIPWTSGMTGIAYDPSKITRPITKLADLWDPAFKGKVGMYSDTQELGNFGMMALGIDPAKSTPDDWKKAAAKLKEQKSAGIVRNYYDQSYVDALGKGEVWITQAWSGDIFQKNVSDGTNFKFVIPDEGGTIWTDNFAIPITAQNPLDAIKLMDFFYEVENAATLAEYINYVCPVPAAQEQIKKDAAKASGDDKAALEAVAASPLVFPAQSDYAKLHYFRDFATVAEQQEYQSIFEPIVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS38</accession>
    <name>U5VS38_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359138.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39607">
        <property type="protein sequence ID" value="AGZ39607"/>
        <property type="gene ID" value="AFR_06590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1343"/>
    <dbReference type="HOGENOM" id="CLU_171888_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046251">
        <property type="entry name" value="DUF6284"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19801">
        <property type="entry name" value="DUF6284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="87" mass="9705" checksum="D80442EACD15486D" modified="2014-01-22" version="1">MLHNLHHNADKDLDGPTSADLAAIAVEWPQIQADIDMLADPDAVDALVDQIYALERGYTPRAWRSRRRAEARITRTALRAASRRTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS55</accession>
    <name>U5VS55_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357444.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01060"/>
    <dbReference type="EnsemblBacteria" id="AGZ38501">
        <property type="protein sequence ID" value="AGZ38501"/>
        <property type="gene ID" value="AFR_01060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.211"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_10_0_11"/>
    <dbReference type="OrthoDB" id="1900922at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="135" mass="14865" checksum="95292F8B306BB898" modified="2014-01-22" version="1">MITKLNVTSLFVLDKEEALEFYVGRLGLEKGSDVQQGSYRWLTVRVPGGGTEISLEEPGPPLHDEETAERLRELVAKGALNGLVLNTGNIQELFESLRAGGFTDFTQEPTEHFYGTDMGLRDPSGNAVRILQQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS92</accession>
    <name>U5VS92_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dnaJ</name>
        <name evidence="10" type="ORF">AFR_07845</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DnaJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07845"/>
    <dbReference type="EnsemblBacteria" id="AGZ39858">
        <property type="protein sequence ID" value="AGZ39858"/>
        <property type="gene ID" value="AFR_07845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1602"/>
    <dbReference type="eggNOG" id="COG0484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_7_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5 6 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="133"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="146"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="163"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="189"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="203"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="7">
        <location>
            <begin position="133"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="378" mass="40323" checksum="E9375D575F30CDBF" modified="2014-01-22" version="1">MAKDYYGILGISREATDDEIKRAYRKLARQFHPDVNPDPEAHEKFKDINAAYEVLSDDQKRQIVDLGGDPLAPGGGGGGAPGGAGPFVGFQDIMDAFFGTAAGGGSRGPRPRTRPGADAILRLELDLVETAFGVEAPITVDTAVLCTQCSGAGTAPGTHLATCETCGGRGEVQSVQRTFLGQVVSSRPCQVCQGHGTIIPTPCPTCAGDGRIRTRRSLTVKIPAGVEDGMRIRLAQQGEVGPGGGTAGDLYVEIHERPHDVYSRKGDDLHCRVTLPMTAAALGTRLTIKTLDSEENIEVKPGTQPASTLRIRGKGVPHLRGQGRGELFVHLDVKTPTKLTADQERMLRDFAKTRGEDIAELSKQGGFFSRMRDAFNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSC5</accession>
    <name>U5VSC5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_08045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359429.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08045"/>
    <dbReference type="EnsemblBacteria" id="AGZ39898">
        <property type="protein sequence ID" value="AGZ39898"/>
        <property type="gene ID" value="AFR_08045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1642"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_7_11"/>
    <dbReference type="OrthoDB" id="870493at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="252"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="287"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="463" mass="49217" checksum="D9BC41E35D8DD1A9" modified="2014-01-22" version="1">MAEVVLRGFGWRHAGRRAWAVRGVDLTVSHGERVLLLGPSGAGKSTLLAALAGLLPEDSGESEGTVEIDGLEPVKARERVGIVFQDPQTQLVMARSGDDVAFGLENRGVPAAEIWPRVSDALERVGFPYPIGRKTAALSGGEQQRLALAGVLALRPGLLLLDEPTANLDPAGGTLIREAVIRAHDPDRTLILVEHRVAEALPLVDRVVVLEPGGGVRADGTPETVFADLGDRLAAEGVWVPGRPLPTHRAVSPPADLLVRAESITVQHRLSAVSLSARAGEALAVTGPNGAGKSTLALVLGGLLAPTSGTVTAFDEPRPPHRWRAAALTQRIGSVFQAPEHQFVTSRVSDELALGPRQLGRTDVASIVDELLERLRLTKLAGANPYTLSGGEARRLSVATALATAPRLLVLDEPTFGQDRRTWIELVELLARLRDDGHGIVAVTHDDDFVTALADRVQHLERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSF9</accession>
    <name>U5VSF9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359464.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08220"/>
    <dbReference type="EnsemblBacteria" id="AGZ39933">
        <property type="protein sequence ID" value="AGZ39933"/>
        <property type="gene ID" value="AFR_08220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1677"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_17_0_11"/>
    <dbReference type="OrthoDB" id="1339160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="145"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="394" mass="42282" checksum="F7E470B263251429" modified="2014-01-22" version="1">MGQLRVGMVGYAFMGAAHSQAWRTVNHAFDLPLSARMSVVCGRDEDKVAAAAAKLGWEGHTTDWRALVERDDIDLVDICTPGDTHREIALAALAAGKHVLCEKPLANSVAEAREMAAAAAEAQANGVRAMCGFNYRRVPAVALMRQLVAAGRIGKIRHVRAVYLQDWIVDPNFPLVWRLRKDVAGSGALGDIGAHIVDLTQFVTGQSITKVSALTETFVRQRPLPTEAAGLSAAGNGSAGFGQVTVDDAALFLARLDGGAVATYEATRFATGRKNGLRVELNGELGSVAFDFERMNELEFYDATLPTTEQGFSRILVTEPEHPYLAAWWPPGHPIGYEHSFTHEMRDLIAAIAAGEDPTPSFADALQVQLVLDAVERSAAASAWVDVEAVLAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSG6</accession>
    <name>U5VSG6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobyrinic acid a,c-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_07340</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07340"/>
    <dbReference type="EnsemblBacteria" id="AGZ39757">
        <property type="protein sequence ID" value="AGZ39757"/>
        <property type="gene ID" value="AFR_07340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1498"/>
    <dbReference type="eggNOG" id="COG1797">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022752_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="PTHR43873"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="4">
        <location>
            <begin position="8"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="GATase_3" evidence="5">
        <location>
            <begin position="278"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="506"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="533" mass="53803" checksum="1B37A1607DB6D811" modified="2014-01-22" version="1">MSAHARLVVSAPSSGHGKTAIAVGLLAACNARGVPAAGFKVGPDHTDAAYLGLAAGRPGRNLDPRLVGAQRIAPLFAHGAAGSQVAVIEGAMGLFDSLTGQPEIDGTAAVAAALRAPVVLVVDVAAMGHSLAALVHGFRMFDEMVHLGGVILNRVASPRHEEMLRTALDDIGMPVLGALRRGDLPAVLPARAQGPVPVAHRTVEAVRAVRRLGEAVASSLDIDRVLALAEGAPPLPGQAWSPLDALAEASGEPPVPPENRPVIALAGGPGAPYTYVETVELLTAAGAEVAIVDPLRDETLPAGTRALMIGAALPEGYAEELSANRRLCAAVSQLAHEGRPIIAEGVGLPWLGRDFDGRPMCGVLDASAQTAAQTIAGYREATAPATSPLAPAGARITGYKQHRAIVTPRAGQVPAWTWSGGNPEGFVWRQVHASQLGLHWAGAPEIARRLVAAALAPLPGAAPRAAATGAAAPRAVASAASAPRVVAPGAAVSSAVAQGTVTLGTVTPAAVTPPDDDPGEQTLSLSAITPPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSK5</accession>
    <name>U5VSK5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="5">3.4.21.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Endopeptidase Clp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">clpP</name>
        <name evidence="8" type="ORF">AFR_07540</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359328.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07540"/>
    <dbReference type="MEROPS" id="S14.008"/>
    <dbReference type="EnsemblBacteria" id="AGZ39797">
        <property type="protein sequence ID" value="AGZ39797"/>
        <property type="gene ID" value="AFR_07540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1539"/>
    <dbReference type="eggNOG" id="COG0740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058707_4_1_11"/>
    <dbReference type="OrthoDB" id="1728970at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="22880" checksum="715FF6822E9C84CA" modified="2014-01-22" version="1">MTDLHIPASPTLRGDSLSGNLDDSVYNRLLRERIIFLGSEVTDQVANRICAQLLLLSAEDPERDINLWINSPGGSVYSGMAIYDTMQFIDNDVSTVAMGMAASMGQLLLCAGTKGKRYSLPHARIMMHQPSGGMGGTAADIAIQAEQMLYTKRMFQERVAFHTGQDQAQIEADSDRDRWFTAEEARNYGFIDKVITGATQVPEGAGTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSR9</accession>
    <name>U5VSR9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heat-inducible transcription repressor HrcA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">hrcA</name>
        <name evidence="8" type="ORF">AFR_07840</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HrcA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359388.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07840"/>
    <dbReference type="EnsemblBacteria" id="AGZ39857">
        <property type="protein sequence ID" value="AGZ39857"/>
        <property type="gene ID" value="AFR_07840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1601"/>
    <dbReference type="eggNOG" id="COG1420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050019_2_0_11"/>
    <dbReference type="OrthoDB" id="1510750at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00081">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002571">
        <property type="entry name" value="HrcA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021153">
        <property type="entry name" value="HrcA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023120">
        <property type="entry name" value="WHTH_transcript_rep_HrcA_IDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34824">
        <property type="entry name" value="PTHR34824"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01628">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08220">
        <property type="entry name" value="HTH_DeoR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005485">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00331">
        <property type="entry name" value="hrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0678">Repressor</keyword>
    <keyword evidence="3 5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="7">
        <location>
            <begin position="17"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HrcA" evidence="6">
        <location>
            <begin position="104"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="37131" checksum="22E20525AA118766" modified="2014-01-22" version="1">MSLDDRKLEVLRAIVEDYVATQEPVGSKSLVERHQLGVSPATVRNDMAVLEEEGYIRQPHTSAGRVPTDAGYRLFVDRLSRVKALSPAERRAIERFLVGAVDLDDVVHRTVRLLAQLTRQVAVVQYPSLARSSVRHLELVPISTTRLMLVMIADTGRVEQRLVELPGVTATDDVLDMRRKINAKLNGEKLSDTPPLVQALVDECDPEKRTTMACVASVLLETLVERSEERLALAGTANLTRGGVLDFQGTLRPVLEALEEEVILLKLIGEVEPSTTRVRIGDENEIDNLRAASVVSTGYGPGTTIVGGLGVLGPTRMDYPGTIATVRAVARYVGDLLAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTI4</accession>
    <name>U5VTI4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cinorf13 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03410"/>
    <dbReference type="EnsemblBacteria" id="AGZ38971">
        <property type="protein sequence ID" value="AGZ38971"/>
        <property type="gene ID" value="AFR_03410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.686"/>
    <dbReference type="eggNOG" id="COG2856">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169049_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13055" checksum="808B100FEA7DD1CA" modified="2014-01-22" version="1">MPGLTAGAPCGLWISVPTADYVFYDPGTSRLHAEHIVLHELGHMLSGHTTGLDVSGGALARLVPDLDPSTVTTILGRVSYTTAQEREAEMLASLIRARSGPETTGDERPGGTLGRVADVLNFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTI7</accession>
    <name>U5VTI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10095"/>
    <dbReference type="EnsemblBacteria" id="AGZ40308">
        <property type="protein sequence ID" value="AGZ40308"/>
        <property type="gene ID" value="AFR_10095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10095"/>
    <dbReference type="eggNOG" id="ENOG5033AGQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084738_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25688" checksum="EF7E393715F4400E" modified="2014-01-22" version="1">MNPSQEPAPQHYEQAGYSYGPPPPEVVAWEQPRPLRRSWSRMVSVAVLLTVALAVAGAPLGLLWSWLSPSVPVINAGQNGIVVNDPSPEEYIAADGWFTLLGFGFGLLVAVLIWLVLRRDRGPALLLGVAFGGLACSVVAWQVGRLVGLSAWNSWQDTSAPGDTYSRPPDLQAYGALLVIAFAAVIVTTLLAGWSNDPDLERPGAKPGYGNNLDDELSSGSPDGPGPTAAPAPPAPWPADPPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTP2</accession>
    <name>U5VTP2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylenetetrahydrofolate reductase</fullName>
            <ecNumber evidence="10">1.5.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_09725</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:19821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19817"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.20" evidence="10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359912.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09725"/>
    <dbReference type="EnsemblBacteria" id="AGZ40234">
        <property type="protein sequence ID" value="AGZ40234"/>
        <property type="gene ID" value="AFR_09725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1982"/>
    <dbReference type="eggNOG" id="COG0685">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025841_0_0_11"/>
    <dbReference type="OrthoDB" id="1425269at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106312">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106313">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADPH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004620">
        <property type="entry name" value="MTHF_reductase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00676">
        <property type="entry name" value="fadh2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="6 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ40234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="33216" checksum="662ED94B7C1F0359" modified="2014-01-22" version="1">MSLGLPSRLPTSPAIGELIRRPDPTFSFEFMPPKSREGEQLLWQTIRELETLRPDFVSITYGAGGSTRDTTVAVTERVATETTLLPMAHLTAVGHSVAELRNVIGRLAGAGIRNVLAVRGDPPGDPNAEWVRHPEGVLYAEDLVRLLRESGDFSVGVAAFPYKHPRSADIESDTLNFIRKCRAGADFAITQMFFEADEYLRLRDRVAAAGCDTPIVPGVMPVLRMATIARAAQLSGAPFPPGLLSRFERIADDADGVRRLGIDLCSEMCQRLIDEGVPGIHFITMNRSTATREVWQHLAANVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUB4</accession>
    <name>U5VUB4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358232.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04685"/>
    <dbReference type="EnsemblBacteria" id="AGZ39226">
        <property type="protein sequence ID" value="AGZ39226"/>
        <property type="gene ID" value="AFR_04685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.946"/>
    <dbReference type="HOGENOM" id="CLU_1998977_0_0_11"/>
    <dbReference type="OrthoDB" id="2087340at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13770" checksum="4B3F4B57E62419DB" modified="2014-01-22" version="1">MGYELRDFLWAETRREQPDLPESPWAEGLTSDDRRQAWLALLAALTTAEREVARLATVAAERAAEHGADYPDLGAAAGMTRQGARRRWPGLSRRGLRGQRTRMSDVATDRRLAADPVNLAADKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV01</accession>
    <name>U5VV01_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11640</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360638.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11640"/>
    <dbReference type="EnsemblBacteria" id="AGZ40617">
        <property type="protein sequence ID" value="AGZ40617"/>
        <property type="gene ID" value="AFR_11640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2371"/>
    <dbReference type="eggNOG" id="COG3795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130902_0_0_11"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="3">
        <location>
            <begin position="3"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="136" mass="14444" checksum="17AD671C9CBCD2B9" modified="2014-01-22" version="1">MSKYMLIMRGTDASNAAMMASIDEAMAATGQFIEEMIKAGVLLAAEGLDEPARGVVVDFSGESPVVTDGPYGSTRELFGGYFLLEVASRQEAVDWATRFPAVTGSKFEIRRVAGSDEAPPAYEPATEDRAASDNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV10</accession>
    <name>U5VV10_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12060</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12060"/>
    <dbReference type="EnsemblBacteria" id="AGZ40699">
        <property type="protein sequence ID" value="AGZ40699"/>
        <property type="gene ID" value="AFR_12060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2455"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_642333_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="110"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="39169" checksum="7A8FCDC3CB76854C" modified="2014-01-22" version="1">MQQLASVHLQGGDQSGARRVLEEAFAAGQWRLGDADPVMLAISFDLGVVAEEMENRHEARRAFTRVATHGPAALGADHWAVVRARSYLGEDPPTVRMELSATDLQRIQVPPQRKAPQDQQVPVAEPAPRHFPAPAPTPQPQPSYERPAPPPQPSYQQPAPRPSYEQPTPQPSYQQPAPQPSYDQPEPRFYEQSRFEQQPMPQIISAPPITSAAPAQPDSSYARKAPAMFAAIAAVFGVIIAVVALVIVIASRGDDPDSDVPTLAGPAPSDVRMRDYGSSVQLFWSDPANGKASFVVTGGRPGQQLQPMGQVGPTTTSFDLNGLNADLDYCFAIVAVYTTSQFSTSPQTCTSRSTGSVAPSPSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVP6</accession>
    <name>U5VVP6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-directed RNA polymerase II subunit RPB1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07505</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359321.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07505"/>
    <dbReference type="EnsemblBacteria" id="AGZ39790">
        <property type="protein sequence ID" value="AGZ39790"/>
        <property type="gene ID" value="AFR_07505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1532"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094102_0_0_11"/>
    <dbReference type="OrthoDB" id="1687438at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="21316" checksum="6CF4C997D30AB7A1" modified="2014-01-22" version="1">MPEQPQWSERTLDMPPQDPWAEPPTSPVQPRPASAAPPQPSPFSSGRAAVNPHPRTQPFAAEHEPTGTGWPGEQTPQERHSLSWHIGQLRRGGEWSFAGLLFAFVCWGIWAISSDGDLTAPIVVFIVSVLVAGGLFALSRLIGRLVLERQFGRVRRTARGSHVVAGLFLVGVGIAHLQQTEWVMTAFNWVAGLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVQ4</accession>
    <name>U5VVQ4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FR47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07555"/>
    <dbReference type="EnsemblBacteria" id="AGZ39800">
        <property type="protein sequence ID" value="AGZ39800"/>
        <property type="gene ID" value="AFR_07555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1542"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112477_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013653">
        <property type="entry name" value="FR47"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08445">
        <property type="entry name" value="FR47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FR47" evidence="1">
        <location>
            <begin position="104"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="18073" checksum="5F94965D4FE0FDB1" modified="2014-01-22" version="1">MAADVDQRWLDSWVDVDDNLSAPLGALFLSALEERLRLVSGTIDVVLLAPPATGEPPLPLTPVTDSEHRRVRRARRYRQDVEVWTSGPGTLVLGRGLAGRWEVAVEVDEDGRNRGLGRSLAAAARHLVPEGRPVWAQVAPGNAASLRAFLAAGYTPVGGEVLLMPVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVS5</accession>
    <name>U5VVS5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cobD</name>
        <name evidence="11" type="ORF">AFR_07285</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07285"/>
    <dbReference type="EnsemblBacteria" id="AGZ39746">
        <property type="protein sequence ID" value="AGZ39746"/>
        <property type="gene ID" value="AFR_07285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1487"/>
    <dbReference type="eggNOG" id="COG1270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_1_2_11"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="PTHR34308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00380">
        <property type="entry name" value="cobD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ39746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="29942" checksum="8365254F7C26A26D" modified="2014-01-22" version="1">MAGFGQAAGALERRLYAPNRRAGAAFTAVAVGVPVVAGLAAAVVTRRQPVVRAALVAAATWTVLGGRTLRRESAVMAGHLEREDLTAARGRLNHLCGRDPSTLDQPELARATVESVAENTSDAVVAPLIWGGLLGLPGLLGYRAANTLDAMVGHRSPRYARFGTPAARLDDLLNLVPARLTGLLTIAAAPLVRGKQAEALRVWRRDRNDHPSPNAGQCESAMAGALGVRLGGRNVYFGRTETRPFLGDGPRPAAGDLRRAARISGAVGAGAAVLAAGVALVRSGLVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVY6</accession>
    <name>U5VVY6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361088.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41029">
        <property type="protein sequence ID" value="AGZ41029"/>
        <property type="gene ID" value="AFR_13715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2785"/>
    <dbReference type="HOGENOM" id="CLU_2598121_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="79" mass="8397" checksum="E60ADA86B302DDB8" modified="2014-01-22" version="1">MPIGQLTGLPGHEADLEAELHRHFTAILPMTVELPPQNLEAILAGGREMVRRQRLRAVIVVAVLVVPVAVAGLMTGAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW37</accession>
    <name>U5VW37_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_13680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361081.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13680"/>
    <dbReference type="EnsemblBacteria" id="AGZ41022">
        <property type="protein sequence ID" value="AGZ41022"/>
        <property type="gene ID" value="AFR_13680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2778"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_3_11"/>
    <dbReference type="OrthoDB" id="787596at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027399">
        <property type="entry name" value="ADH_N_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13823">
        <property type="entry name" value="ADH_N_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ADH_N_assoc" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="ADH_N" evidence="4">
        <location>
            <begin position="27"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="ADH_zinc_N" evidence="3">
        <location>
            <begin position="198"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="391" mass="42178" checksum="E944DA1BF7B07FC6" modified="2014-01-22" version="1">MRANTWSGRNKVEVRDVPDPKILNNRDAIVRITSTAICGSDLHLVDGYVPTMQDGDILGHEFMGEVIEVGSGVPGEKLRVGDRVVVPFPIACGACAACAAELYSCCENTNPNAGIAEKMFGHPVAGIFGYSHLTGGFAGGQAQYARVPFADVGPLKIESDLTDEQVLFLSDILPTGYMGAEMCDIQSSDVVAVWGAGPVGQFAMDSARVLGAAKVIAIDKEPYRLQMAERAGHIPVNFDEVDVRSTLLELTGGRGPDKCIDAVGLEADHGSAHIAAYDRVKQAVRSETERPHALRQAIMSCRSGGVVSVIGVYGGLLDKFPAGAWMNRSLTLRTGQCHVQRYMKPLLERIERGEIDPTRIITHTLALDEAEQGFNLFKNKQDNCEKVVLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWD5</accession>
    <name>U5VWD5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359507.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08435"/>
    <dbReference type="EnsemblBacteria" id="AGZ39976">
        <property type="protein sequence ID" value="AGZ39976"/>
        <property type="gene ID" value="AFR_08435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1717"/>
    <dbReference type="HOGENOM" id="CLU_1965828_0_0_11"/>
    <dbReference type="OrthoDB" id="1846606at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="14631" checksum="1C43366370E378FD" modified="2014-01-22" version="1">MSPSPPPAGFGDYLREAIHAAGFPTPTHFARTVGTDPSVVLRWLSEEQRPTIRSIERIAPVLGRTINELVVAAYPDRLGGPAPVTPAVHPLVSELGRILGEDSPISAADRAALETVLDRMFEPYRKVVRRRRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWH1</accession>
    <name>U5VWH1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tetracycline repressor protein class C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08610</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08610"/>
    <dbReference type="EnsemblBacteria" id="AGZ40011">
        <property type="protein sequence ID" value="AGZ40011"/>
        <property type="gene ID" value="AFR_08610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1752"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003012">
        <property type="entry name" value="Tet_transcr_reg_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00400">
        <property type="entry name" value="TETREPRESSOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="6">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="221" mass="23842" checksum="20AE638A4A1FA953" modified="2014-01-22" version="1">MPLSREKIAAAAIRLADAHGLDGLSIRKIAKELGVGPMRLYDYVGNRSELLDLMVDVVYARIAEADRHPDWRATVLAVAHATREAALDHEWFADVLGGRPHLGPHALAVGETTAAALSQASGVRDLEDLQRALGALNAFIVGAVRREITERRTARSTGTDEAAWQAGLGPYLTRMLETGRYPTVARLVLEGAHLDAGETFEHNLTTVVDGITAARPPLRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWS3</accession>
    <name>U5VWS3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15210"/>
    <dbReference type="EnsemblBacteria" id="AGZ41324">
        <property type="protein sequence ID" value="AGZ41324"/>
        <property type="gene ID" value="AFR_15210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3088"/>
    <dbReference type="eggNOG" id="COG1533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057301_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="394" mass="43678" checksum="F2CE74FD2DD74E48" modified="2014-01-22" version="1">MPLTGIDLNQPLAPAAPAAPHSASGEAAATHDLAAVAPPARPARRWTPKRVVATPAAYDHAHGRRIMELVESQGIEVERLRSNRLTGLRGETDRETYARAKATMAIVVSPPSRRKLQPIAPSADWRFDLAEGCPAHCQYCYLAGSLSGPPVTRVYADLDDILAGLADYEGRGTITSRRDARRDEGTTFEASCYTDPLALEHLTGSWRRAVEHFGAWDAPVQLRWTTKFDDVDTFVGLPHHGRTRVRLSVNCKPVTTRVEGGTSRLEDRLAALRRLARDGYPVGVTIAPIIAVPDWRDHYTELLDGIADAVRDVPGLDLTAELITHRFTPGSKEVLLGWYPRTKLEMDEESRSRKHGKFGAVKYVYPAATMAELRTFFHTELATRLPSCRILYWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX16</accession>
    <name>U5VX16_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LuxR family two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_16255</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361608.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16255"/>
    <dbReference type="EnsemblBacteria" id="AGZ41533">
        <property type="protein sequence ID" value="AGZ41533"/>
        <property type="gene ID" value="AFR_16255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3298"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="144"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="22923" checksum="BF0F73F686DB2B78" modified="2014-01-22" version="1">MRVMLADDAVLFREGLARILTDGGFTVTGQAGDGPTLIDLVRADPPDAAVIDLRMPPGHAAEGLEAAAAIRAIAPGVGLMLLSQYVEVHHALRLMTEFDGGVGYLLKDRVSDLGSFGADVRKVAGGETVIDPELVTRLVARRRARDPLEGLTERERRVLALMAQGLSNTAVAGDLHLAVKTVEAHVGSIFTKLGLVQHDREHRRVLAVLTFLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX94</accession>
    <name>U5VX94_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="8">IGPS</shortName>
            <ecNumber evidence="8">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpC</name>
        <name evidence="10" type="ORF">AFR_10305</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360142.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10305"/>
    <dbReference type="EnsemblBacteria" id="AGZ40350">
        <property type="protein sequence ID" value="AGZ40350"/>
        <property type="gene ID" value="AFR_10305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2098"/>
    <dbReference type="eggNOG" id="COG0134">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034247_0_1_11"/>
    <dbReference type="OrthoDB" id="1789381at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_A">
        <property type="entry name" value="IGPS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_B">
        <property type="entry name" value="IGPS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="PTHR22854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="IGPS" evidence="9">
        <location>
            <begin position="2"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28156" checksum="48288CC671E9DDFB" modified="2014-01-22" version="1">MLEEILAGVREDVAARQEQLSLEQVRDLAAAAPPAIDAYAALRKPGVAVIAEVKRSSPSKGALADIPDPAELAGEYAAGGARCISVLTEGRWFGGSLEDLAAVRAAVQVPVLRKDFVVSSYQIHEARAHGADLVLLIVAALEQNVLMGLLERIESLGMTALVEVHNEEEADRALDAGAQVIGVNARDLRTLEVDRSVFERIAPGLPNNVVKIAESGVRGPHDLIRYASAGADAVLVGEGLVTKKSPRDAVAELVNAGNHPATPRPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX98</accession>
    <name>U5VX98_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_16480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16480"/>
    <dbReference type="EnsemblBacteria" id="AGZ41578">
        <property type="protein sequence ID" value="AGZ41578"/>
        <property type="gene ID" value="AFR_16480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3342"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="24"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="493" mass="50668" checksum="6DFF1F630724400E" modified="2014-01-22" version="1">MTQTLQGPGTEPVRPEFKSGGSLLVVYLSLGGLAFAVLQSLVAPAMSTIARDLNATTGDISWILTAYLLSASVLTPILGRLGDMVGKRRVLIGVLAVLAAGTLLAALAPNLAVLIVARALQGAAGAILPLSIGIVRDSLPRERVGVTVGLLSAIFGVGAGVGIVSAGPIVEHLSWHWLFWLPLVLVVVALLGAIFGMKESPVRTPGRLDVLGAGILSISLVSLLLAISKGQSWGWGDTRTVSLLAVGALAMIVFVAVELRVKEPLIDMRLMKIRGVWATDVVALILGFAMFGTFLLVPTLLQLPAATGYGFGKSVSQAGLFLLPTVLMMVVFGPLAGLLDRRYGPKVPMFLGAVSVVVAFALPALGHGAIWQVLASGVLTGAGIGLAFAAMSNAIIESVPAAQTGEASGVNTIARTIGSSIGTAVVAAVISSQSTPQGLPTDKAFTYGFWVCAAVAVLAVAASLALPSARRRHEQAVATGVSDLPPEPAELRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXB9</accession>
    <name>U5VXB9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">DNA polymerase I</fullName>
            <ecNumber evidence="2 11">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">polA</name>
        <name evidence="15" type="ORF">AFR_10430</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2 11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360189.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10430"/>
    <dbReference type="EnsemblBacteria" id="AGZ40375">
        <property type="protein sequence ID" value="AGZ40375"/>
        <property type="gene ID" value="AFR_10430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2125"/>
    <dbReference type="eggNOG" id="COG0258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0749">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004675_0_0_11"/>
    <dbReference type="OrthoDB" id="1220182at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018320">
        <property type="entry name" value="DNA_polymerase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="PTHR10133"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00593">
        <property type="entry name" value="pola"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="53EXOc" evidence="13">
        <location>
            <begin position="8"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="3'-5' exonuclease" evidence="12">
        <location>
            <begin position="309"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="POLAc" evidence="14">
        <location>
            <begin position="653"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ40375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="897" mass="98403" checksum="442A4C75657F6DC0" modified="2014-01-22" version="1">MSDDAQTPRLLLLDGHSLAYRAFFALPVENFSTQTGQPTNAVFGFTSMLINMLRDEKPTHIVVAFDVSRVSFRTEKYADYKAGRAETPQPFQGQVSLVKEILEALRIPVVEKPGYEADDVIGTLACQARDAGMEVVISTGDRDAFQLAGEHVTILYPVRGVSEVWRMTPEAIETKYGVPPGRYRDKAALVGEASDNLPGVPGVGDKTAAKWINQYGGLEGIIAHVDEIKGKAGENLRAHLADVMRNYELNALVCDLELPLRPEDVKWQGWDREAVHQVFDALEFRVLRERLYSYLEAVEPEAESGFDLSGTVLGVGEVAGWLTEHAREAPVGVAVTGRFGRGTGELTGIAVATGDGPAAWFDPATLDESDERAVAAWLADADRPKVIHEAKPAMLAFRAHGWTLTGVRVDTALAAYLAKPDQRAYDLTDLALRYLKRELRVDAPQDGQLTLDGLGEEEGTAEQNVMLRARATLDLADTITEELSRDDGASQRILAEVEQPLAIVLGEMEHVGIAADTEYLSELEAHFAAEVKSAQQGAHEVVGREFNLGSPKQLQEILFTERGLPKTKKIKSGYTTDADALQSLFAQTGDPVLEHLLRHRDVAKLKSTVDGLLKSVSDDGRIHTTFNQTVAATGRLSSTDPNLQNIPIRTEEGRRIRRAFVVGEGYEQLMTADYSQIEMRIMAHLSGDEALIAAFNSGADFHAATASSVFHVPVEEVVPDQRRKIKAMNYGLAYGLSAFGLSNQLTITTEEARTLMDEYFQRFGGVRDYLQAVVRRAVQDGYTATILGRRRYLPDLSSDNRQRREMAERMALNAPIQGSAADIIKIAMLNVDAALRASDLTSRMLLQVHDELVFDVAPGERAALEELVRREMGGAYPLSVPLEVSVGVGQDWNGADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXD7</accession>
    <name>U5VXD7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_16725</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361719.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16725"/>
    <dbReference type="EnsemblBacteria" id="AGZ41623">
        <property type="protein sequence ID" value="AGZ41623"/>
        <property type="gene ID" value="AFR_16725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3391"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_11"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="27"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="308"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ41623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="41847" checksum="9F3ED0A32F583A5B" modified="2014-01-22" version="1">MDERSRLSLRDLVGEAVSGILQRPGRSTLTMLGTVLGIGAMVAILGLTTTAGGQIDRRFTALAATEVTVEDVGADQVSHEMSFPADAGSRIRQINGVVRGGVLWPLPLRKATISGVPEVSGESSGLALYAAEPDALATLHPVITTGRTFDAFHVSRRERVAVLGIGAANRLGITRLDAYPAVFINGSPYTVIGIISELERQPELLLGVILPSSTALTAYGPPVDQPAKMIVETRLGAARVVAQQAPLALRPDAPDRFRAIAPPDPQSLRGSVTSDLNVLFVVLAAITLIIGGVSIANTTFVAVLERTGEIGLRRSLGARRRHIAAHFLAESAALGTLGGLVGTSLGVVVVVVVALIQDWTAVLQPWTVLPSPAAGTLVGVAAGLFPAIRAAKIEPVEALRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXE1</accession>
    <name>U5VXE1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361729.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16750"/>
    <dbReference type="EnsemblBacteria" id="AGZ41628">
        <property type="protein sequence ID" value="AGZ41628"/>
        <property type="gene ID" value="AFR_16750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3396"/>
    <dbReference type="eggNOG" id="ENOG5031XXG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1064085_0_0_11"/>
    <dbReference type="OrthoDB" id="1105530at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="26920" checksum="B6144E7C4D70ACA3" modified="2014-01-22" version="1">MTGEHHLRDLLEHATTHEPPPGAGTAEVFSRARSIQRRRRAGVGLAGAATLGVLVAAGVTWSGPAATPEPTDGVAPATAAPAGTPSAQARTPLTATTALDTLRTLLPKGSRVSNEVARKGFASLVVKDAGGRTTVEVNVDPEFSTDAGKASPADLLDRYDCAKRTDPQGTECTAATRDDRTRIVSIDGPADEPGASEIVRRQVDVLGPDGQRVVVTVWNAVDVSSGEVTRPAPALTGAQLQEIATSKRWNTAPTGEGGAPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXU7</accession>
    <name>U5VXU7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha-galactosidase</fullName>
            <ecNumber evidence="5">3.2.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Melibiase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_17105</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361873.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17105"/>
    <dbReference type="EnsemblBacteria" id="AGZ41699">
        <property type="protein sequence ID" value="AGZ41699"/>
        <property type="gene ID" value="AFR_17105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3470"/>
    <dbReference type="eggNOG" id="COG3345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013093_3_2_11"/>
    <dbReference type="OrthoDB" id="469334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052692">
        <property type="term" value="F:raffinose alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000111">
        <property type="entry name" value="Glyco_hydro_27/36_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="PTHR11452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00512">
        <property type="entry name" value="ALPHA_GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666191" description="Alpha-galactosidase" evidence="6">
        <location>
            <begin position="27"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="7">
        <location>
            <begin position="404"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="523" mass="55644" checksum="32C361B4CD3CC5D6" modified="2014-01-22" version="1">MRIRRIVLSLLLVVAAVPALQSPAAAIDNGLARTPPMGFNNWNSTWCRAEFNEAMIKGIADLFVDKGLKAAGYQYVNLDDCWAQRQRDAQGNLVPDLTRFPNGIKALADYVHGKGLKFGIYTSAGTATCAPEGFPGGLDHEDQDARLFASYGVDYLKYDNCNNQGRDAVQRYTRMRDALAATGRPIILALCEWGENQPWRWAGAVGNLWRTTGDIEDNWGSVLNIIRQNSVLAAYAKPGFWNDPDMLEVGNGGMTDTEYRTHFSLWSMMSAPLLIGSDLRTASASTLSILTNTAVIAIDQDPLGKAAYVVRNSGGQWVFSKVLANGDRAVALFNENGSATTISTSAAQAGMNAAGSYTLRDLWSGSTTTTSGTISATVPAHGTVVYRVSGGSAYAGHSLVGQGSGQCLDVHDNLVAGDAPVEIFGCNGGANQRWNATPAGELRVYDGTRCLDVTNGGTAGGTRVIQWECNGGANQQFRLNADGTITAVQSGQCLDVSGGGSAPGTAIIIWPCHRAANQIWSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXY5</accession>
    <name>U5VXY5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_11330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11330"/>
    <dbReference type="EnsemblBacteria" id="AGZ40555">
        <property type="protein sequence ID" value="AGZ40555"/>
        <property type="gene ID" value="AFR_11330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2309"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_7_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="13"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="158" mass="17397" checksum="25189340F491C8C1" modified="2014-01-22" version="1">MKGLRARVGHDLIMFPTVSAIVLNDRGEILLGQRSDNHQWSVIAGMMDPGEQPADALVREVEEETGVQVTIERLAGVALHEVTYVNGDRCHMVNTWFRCRAIGGEARVNDSESIAVGWFAPDALPEMNAFALVRIRTSLEENAPAWFAQPGEELLTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY01</accession>
    <name>U5VY01_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase GCN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362002.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17430"/>
    <dbReference type="EnsemblBacteria" id="AGZ41764">
        <property type="protein sequence ID" value="AGZ41764"/>
        <property type="gene ID" value="AFR_17430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3534"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139687_0_0_11"/>
    <dbReference type="OrthoDB" id="1983082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16683" checksum="41337DABB17C7A63" modified="2014-01-22" version="1">MDRLRPATTADTTAIRELVRLAFSRYTPRIGEEPAPIGADYASLTARGLCWVAELDGRIGGLVVLVPAGDHLEVETLAVAPDLQGRGVGTRLLALADEQATAAGFTEIRLCTNEKMTENLAFYPRHGFRETHRAEQDGFCRVFFVREIVAPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY67</accession>
    <name>U5VY67_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11435"/>
    <dbReference type="EnsemblBacteria" id="AGZ40576">
        <property type="protein sequence ID" value="AGZ40576"/>
        <property type="gene ID" value="AFR_11435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2331"/>
    <dbReference type="eggNOG" id="ENOG5032DG0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1068036_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021862">
        <property type="entry name" value="DUF3472"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11958">
        <property type="entry name" value="DUF3472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665866" evidence="1">
        <location>
            <begin position="30"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="27958" checksum="3BF3939DE128C175" modified="2014-01-22" version="1">MMRRTGARVAAILLLAVAALGGVPTSATAAGQHQNANLYAYWNFHGRNGFWNVDQQVRIAQKASSSYWAMNFGFTATPSDGGYMGLQTNGRRFNGSTGDTAIFSLWNATATRGANCGRFGGEGTGRSCRIAYPISTGAYYRMRIWRLEADSAGQWWGAWILNPATGRDSAIGQIRVPRAKNLLTTPMNFSEYFGPARTCDTVPVSVAYFTQPTANSRGGGRYDYGSVFSSSHRGSCTGGSVRVATVGSTRAAVIRMGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYF8</accession>
    <name>U5VYF8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbohydrate ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362297.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18230"/>
    <dbReference type="EnsemblBacteria" id="AGZ41924">
        <property type="protein sequence ID" value="AGZ41924"/>
        <property type="gene ID" value="AFR_18230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3700"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052600_0_0_11"/>
    <dbReference type="OrthoDB" id="1264541at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665873" evidence="1">
        <location>
            <begin position="32"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="446" mass="47482" checksum="F9666397F0684885" modified="2014-01-22" version="1">MTYRNTDGPRISRRALLALGAGVTAAPVLSACGGVSTSGTSGEAGTVTLMSNQFSPVEEKQRFEKILKDRVTAEKVAFNQVDTGIFSSTIQTQVEAGKVQVGVVGALHGDLAQHTSRLIDLDDVIGSLGDRGFAPELTGLAKLGGGTTKYVPWMQASYVLAVNKKALEWLPSGADVQKLTYDQLLSWTTAARQGNGGKPVFGIPAGVKGLYHRFFQGFLLPSFTGAQITAFRSAEAVTAWEYMKDLWAQTARGSTTYDNLQEPLQRGEVLIGFDHVARLVGAPADKPDDWLMVPAPTGPKGLGYMLVLAGLGIPKGAPETDKAKEVIKALTTPEVQLDVLRQNAFFPVTNAQVPADLPGAIALAQQAIKAQRDAPKALLSLPPVGLGARDGEVGQIFKNCFKEICLDNKPVRQVLDAQAGQLNAILDELKIPCWQPDPAGNPCRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYI7</accession>
    <name>U5VYI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362167.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17845"/>
    <dbReference type="EnsemblBacteria" id="AGZ41847">
        <property type="protein sequence ID" value="AGZ41847"/>
        <property type="gene ID" value="AFR_17845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3620"/>
    <dbReference type="HOGENOM" id="CLU_2679339_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="74" mass="7864" checksum="EAA1745B51C77FCA" modified="2014-01-22" version="1">MTESTAGSDATAGEQPEVEPTEGDQPEEEFVNRAARRAKGKKAGGGPEVFTKGQRPMGRGSVQSPRQYGNRRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ53</accession>
    <name>U5VZ53_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chelated iron transport system membrane protein yfeD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_18805</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362410.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18805"/>
    <dbReference type="EnsemblBacteria" id="AGZ42037">
        <property type="protein sequence ID" value="AGZ42037"/>
        <property type="gene ID" value="AFR_18805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3820"/>
    <dbReference type="eggNOG" id="COG1108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_4_3_11"/>
    <dbReference type="OrthoDB" id="1421296at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="28550" checksum="C33F2E68EF8A5A94" modified="2014-01-22" version="1">MFEAPFMVRAFAELALLALICGPVSVFVFTRRLSFVADALTHTVFPGVVIGFVAAGSEGIFGGALVAGALTAVALTLLTRGGLLSDDAATAVVLTAMFSVGVALVSRRSSYTSDLTSFLFGRLLTVTTRQLAETAVLAVVILALVALGARAWTFRAFDPDGARAAGYRTTLLDLWLNLVVALVVVAAVRAVGTVLVIALLVVPAAAARTVTDRLPVMAALGTILTLLAGYAGLWVSWTASVGYGLRLTSATAVVLALVLAYAVLLGAGRLRTGRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ84</accession>
    <name>U5VZ84_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endoglucanase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18915</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18915"/>
    <dbReference type="EnsemblBacteria" id="AGZ42057">
        <property type="protein sequence ID" value="AGZ42057"/>
        <property type="gene ID" value="AFR_18915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3842"/>
    <dbReference type="eggNOG" id="COG2730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018488_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665374" description="Endoglucanase" evidence="5">
        <location>
            <begin position="31"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="6">
        <location>
            <begin position="351"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="326"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="454" mass="47480" checksum="ADA4CAFC8337966E" modified="2014-01-22" version="1">MRRRKLFGGLAALFLAVTTVVTVAAQPAQAAAGFTVSGGRILDANGNNFVMRGVNHAHTWYADKLGSLADIKALGANTVRVVLSSGDRWTRNDAANVTTVINTCKANKLICVLEVHDTTGYGEDSAATTLAKAVDYWISIKSALAGQERYVILNIGNEPYGNTNAAAWTADTKSAITRLRAAGFEHSIMVDAPNWGQDWQFVMRDNAASVFAADPQRNTIFSIHMYGVFDTAAEITDYLGRFRTAGLPIVVGEFGHNHSDGNPDEDTILATAQAQGIGYLGWSWSGNGGGVEYLDLVTNFNAAALTSWGERLFNGANGIKATSREASVYGGTTPPTDPTSPSTPPPSPTTPPPAGKQCTATYAMTGSWGSGFQGAVTVTAGSAAISSWTVTWTWPSGQQFTNTWNATVTSSGSAITARSLNYNGSLPAGGGTSWGFTASSTGTNTAPTATCSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZC7</accession>
    <name>U5VZC7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362606.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19810"/>
    <dbReference type="EnsemblBacteria" id="AGZ42234">
        <property type="protein sequence ID" value="AGZ42234"/>
        <property type="gene ID" value="AFR_19810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4020"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102191_1_0_11"/>
    <dbReference type="OrthoDB" id="1497607at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="18157" checksum="B9D7D21530AFB51D" modified="2014-01-22" version="1">MFLRDIRPADLEACIRMRGDPAMMADLGGPLPRDGIERSFHREVAWAEAGTAWIKMILPTESSPVAGTVTVWQHDGLSEIGWLVLPEFQGRGLAGQAVREILHQAGEDGRWGVIHAYPGTANGPSNSICRSAGFTFVEELDLEFAGRTLRSNHWLVDPGRARAGQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZD8</accession>
    <name>U5VZD8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH-type transcriptional regulator tcmR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362621.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19885"/>
    <dbReference type="EnsemblBacteria" id="AGZ42249">
        <property type="protein sequence ID" value="AGZ42249"/>
        <property type="gene ID" value="AFR_19885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4035"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_2_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041347">
        <property type="entry name" value="MftR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17754">
        <property type="entry name" value="TetR_C_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="15"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="199" mass="22021" checksum="D588CA8AADC572CB" modified="2014-01-22" version="1">MTSSLPTGLRERKKAKTRAAIRAHAMRLFDEQGYAATTVDQIAEAADVSQSTFFRYFPTKEDVVLADDYDPPMVAALRAQPADVHPIEAIRRSIREIYDQLTPDEWEQERRRQRLIRSVPELQARNTQQYTQIIGLLGAAVAERAGLPPDDFSARVLAGAVIGAGLAVAPHSLLPEQGTDYFTDFERALALIEAGFPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZE1</accession>
    <name>U5VZE1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_13895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41065">
        <property type="protein sequence ID" value="AGZ41065"/>
        <property type="gene ID" value="AFR_13895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2819"/>
    <dbReference type="HOGENOM" id="CLU_2730839_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="8112" checksum="E9F4EE9FBC9F6D82" modified="2014-01-22" version="1">MYYFAAQMRDGMFRERFAELGFDGNAGDIVSVITDETPAATPVTGRMLLVREDETEKCGWRMWWAQSAART</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZM1</accession>
    <name>U5VZM1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Epimerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361219.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14390"/>
    <dbReference type="EnsemblBacteria" id="AGZ41160">
        <property type="protein sequence ID" value="AGZ41160"/>
        <property type="gene ID" value="AFR_14390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2920"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_12_3_11"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30217" checksum="A1425A2C4FD34AC1" modified="2014-01-22" version="1">MRVFITGASGWIGSALTRELLAAGHEVTGLVRSDTSADKVRAAGGTPVRGDMHDHDLLVAEATKADATAHLAFTINFDEFDETIANEVSLIGRIGDAIEGTGKAFFAASGTPTSLGQIATEQDALDPEGPAGARSRTAAAVLALSDRGIRSGLVRMPRTVHGQGDRNGLIAALVGLDRQLGTAAYVGDGRNRWPAVHISDAGRLFLLALEKAPAGAVLHAVGEEGVPMREVAEVIARKTGLPAAPVDPAQLGIFGALLGGDQPASSTATQQLVGWKPTGPTLLDDLEAGYYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZQ4</accession>
    <name>U5VZQ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362845.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21005"/>
    <dbReference type="EnsemblBacteria" id="AGZ42473">
        <property type="protein sequence ID" value="AGZ42473"/>
        <property type="gene ID" value="AFR_21005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4258"/>
    <dbReference type="HOGENOM" id="CLU_2447995_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="89" mass="9217" checksum="8D05A4A542ABD973" modified="2014-01-22" version="1">MQETTVSSFLILLKAVGAAGRPAIVLIAHLPIILIAVGLLPALMIGVSVLPARYAGVLGTTISQLCTWSSRIIATAGVAPLEEAPRTDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZW2</accession>
    <name>U5VZW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362895.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21255"/>
    <dbReference type="EnsemblBacteria" id="AGZ42523">
        <property type="protein sequence ID" value="AGZ42523"/>
        <property type="gene ID" value="AFR_21255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4311"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061176_0_0_11"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="89"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="34627" checksum="994AE55EAC4D682C" modified="2014-01-22" version="1">MDLLVLGGTAFAGRAVVDEARTRGHRITVLNRGTTPPPAGVTALTGDRTRPDGLAALQHGTWDLVVDTWSGAPTAVRDTAKALSGRAGHYTYVSSRSVYADDPVPADENSPTVDASPDTDTYAAAKRGAELAADHHFNGPVLHARAGLILGPHENIGRLPWWLNRIARGGPTLAPGPRDLKLQYIDARDLATWILDAATAGRTGPYDLVSPPGHTTMGELLDTINTVTGGHADLHWTDPQTILDAGIEPWTDLPIWLTGHDYAVLHGGNVTKALSAGLKVRPVTETVADTWIWLRALGGTAPQRPDRPAPGLDPTREAAFLATLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W010</accession>
    <name>U5W010_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361374.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15165"/>
    <dbReference type="EnsemblBacteria" id="AGZ41315">
        <property type="protein sequence ID" value="AGZ41315"/>
        <property type="gene ID" value="AFR_15165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3079"/>
    <dbReference type="eggNOG" id="COG2354">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059391_0_0_11"/>
    <dbReference type="OrthoDB" id="1419056at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008526">
        <property type="entry name" value="YedI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30503">
        <property type="entry name" value="PTHR30503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05661">
        <property type="entry name" value="DUF808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016660">
        <property type="entry name" value="YedI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="313" mass="32756" checksum="D8673F423263FAAB" modified="2014-01-22" version="1">MAGGLVALLDDVAVLARAAAASVDDIGAAAAKAGAKAAGVVIDDAAVTPQYVRDLAADRELPIIKRIALGSLRNKFLIILPAILLLSQFVPWLLTPILMLGGAYLCFEGAEKVWAKIAHHDAHGEDEGAKDEKTLVSGAVRTDLILSAEIMVITLNEVIDEPFWSRLAILAVVAVIMTVLVYGAVALIVKMDDAGLRLAQLPGAIAKFGRGLVKAMPIVLTALTIIGTAAMLWVGGHILLVGTDELGLHFLYEAVHHLEEAAHDATGALGGVVGWLVNTIASAILGLIVGALVVVVMTFTIHRRKPEPAAEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W058</accession>
    <name>U5W058_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosylpyrimidine nucleosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_15095</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15095"/>
    <dbReference type="EnsemblBacteria" id="AGZ41301">
        <property type="protein sequence ID" value="AGZ41301"/>
        <property type="gene ID" value="AFR_15095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3065"/>
    <dbReference type="eggNOG" id="COG1957">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036838_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901564">
        <property type="term" value="P:organonitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="PTHR12304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="SSF53590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IU_nuc_hydro" evidence="3">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="310" mass="32393" checksum="744641A01F620636" modified="2014-01-22" version="1">MIVDCDPGHDDALALLLAVGDPRLQLLGVTTVAGNQTLEKTTRNALRILALAGAGEVPVAAGCDRPLVGELTVAEDIHGGSGLDGPDLDVPVAAIADVHGVELMRRLIMGSAGPVTVVATGPLTNVALLVRTHPEVLPRVRRIVFMGGSTERGNTTPYGEFNIVTDPEAADIVLRSGVPLTMIGLNVTHQALATEEIIAEFRGLGTRLGVVCADLMTFFASTYLRVFGFEHPPVHDPVAVAAVIDASIVRTVAAPVAIELTGTHTRGATVVDLHRRTGEAPDVDVAVGLDVDAFWRLLMAAVRNAGQTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W085</accession>
    <name>U5W085_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_20850</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362814.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20850"/>
    <dbReference type="EnsemblBacteria" id="AGZ42442">
        <property type="protein sequence ID" value="AGZ42442"/>
        <property type="gene ID" value="AFR_20850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4227"/>
    <dbReference type="eggNOG" id="COG3852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_49_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="181"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="255"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="5">
        <location>
            <begin position="335"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="4">
        <location>
            <begin position="473"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="736" mass="79389" checksum="DDF5A6D7ACCB2C28" modified="2014-01-22" version="1">MHGADFDDHTADYWARQIRIGSAIAAGVTLLGCVRVAADWAPADRWLIPWALGAVVLQLAALALPWLRLARDVRIRRSLVLWLVAELPLLFQFARIDDDGLMFYLPGSALIIVATAALYPPLVVVGLGVLALVGYLALLPAVTAGNAVSIVSLLAIMFCLVALTAINSRSRRRLDERRKAAEHRTELLLEAASDAVFAIDVAGEIRYASPSAQRILGMGTDQLSGVALKTLAHPDELAGLGAWMSDLWRVPGGTSRTEARLRRPDGTWMHLDVIGTNRLDDPSLRSAVISLRDIGRRKELEDQLSLQAFTDSLTGLPNRALFRDRLEQAARRPGSDVTVLLIDLDDFKLVNDNLGHNAGDELLATLAGRLRGEMRAGDTLARLGGDEFAILAEDLDAAEAAVLAERLLTAGREQVRLGSRDVSCSLSIGIATGDLPAEQLLRNADLAMYAAKRSGRNAYQLFSPSMSRSVLEEAQQRVDMERGLEQEQFVVLYQPVVDMTTQRLTGVEALVRWRHPDDGLLSPYQFIANAEANGLIVPLGRWVLREACAQLARWRRETPAAAGLKINVNLSARQFQYAGLVGDVAAALADAGIPASALTLEITESMLMEDVTGAIETLGALRQLGVRLAIDDFGTGYSSLNYLKQLPVDIIKIDRTFVESVDTDADDVALVDAVVGLGQALRLQTVAEGIETDGQWAMLQQIGCDQGQGYLFGRPGDAASVAALLARDAAAAPAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0I2</accession>
    <name>U5W0I2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pectate lyase/Amb allergen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_15795</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361500.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15795"/>
    <dbReference type="EnsemblBacteria" id="AGZ41441">
        <property type="protein sequence ID" value="AGZ41441"/>
        <property type="gene ID" value="AFR_15795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3206"/>
    <dbReference type="eggNOG" id="COG3866">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021894_2_1_11"/>
    <dbReference type="OrthoDB" id="660314at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PTHR31683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 2 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665550" evidence="4">
        <location>
            <begin position="33"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Amb_all" evidence="5">
        <location>
            <begin position="77"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="356"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="356"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="371"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="386"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="510" mass="52151" checksum="3C43939B8F40E8CB" modified="2014-01-22" version="1">MRRPHGRLQAGLATLAVGAAIGLALPMSQASAAVGGVTGYATQNGGTTGGAGGATVRATTGTAIHTALCTRATSSTPIIIEVQGTINHGNTAKVSGSSCNTAAGVIELKQISNVTIIGVGSGAVFDQLGIHIRDSSNIIVQNVTVRNVKKSGSPTSNGGDAIGMESTVRNVWVDHVTLEASGGESEGFDGLFDMKDNTQYVTLSYSILRNSGRGGLIGSSESDLSNGFITFHHNAYENLDSRTPLLRGGIAHIYNNSYVSLNESGINSRAGARAKVENNYFKNSKDVLGTFYTDEAGFWQVSGNIFDNVTWSAPGTDNRPAGPNPQSNTTVSIPYAYSLDGASCVPEIVRQTAGANTGLQVSNGNCTPQSPTPTTPTPTTPTPTPSQPSGTNLSIGAGSDGSSKAAGTGYGNVRDGDLTTFWSPAGATGSISIKWGSATTVSRIIIREASGSAGSIGSWQVLNHATGAVLKSGSGAGAITFIPTSLTKITFNITSSTGTPKVAEFETYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Y4</accession>
    <name>U5W0Y4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Non-homologous end joining protein Ku</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ku</name>
        <name evidence="5" type="ORF">AFR_23265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">With LigD forms a non-homologous end joining (NHEJ) DNA repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA nor ssDNA. Recruits and stimulates the ligase activity of LigD.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer. Interacts with LigD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic Ku family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363461.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23265"/>
    <dbReference type="EnsemblBacteria" id="AGZ42923">
        <property type="protein sequence ID" value="AGZ42923"/>
        <property type="gene ID" value="AFR_23265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4715"/>
    <dbReference type="eggNOG" id="COG1273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048975_1_1_11"/>
    <dbReference type="OrthoDB" id="1724780at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00789">
        <property type="entry name" value="KU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01875">
        <property type="entry name" value="Prokaryotic_Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="Ku70/Ku80_beta-barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009187">
        <property type="entry name" value="Prok_Ku"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41251">
        <property type="entry name" value="PTHR41251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006493">
        <property type="entry name" value="Prok_Ku"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SSF100939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02772">
        <property type="entry name" value="Ku_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ku" evidence="4">
        <location>
            <begin position="53"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="261"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="290"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="328" mass="35611" checksum="4405A83650B9AB44" modified="2014-01-22" version="1">MARPIWTGAITFGLVSVPVAMYSATHEHEVSFHQYEKGTSDRIRYQRVNERTGKEVDYDNIVKGTDVGGGQLVLLDQDELDSVAPGRSKSLDLHAFVDLDDIDPIFYQKSYYLAPGSEETAKVYALLRDAMADTNRAAIGTLVMRGKEYLAAIRPDGKLLVLETMYFADEVRDPAKELDDLPRSTAKPAEMKMAVQLIGSMSAKWKPKDYRDSFTDRVNALIEEKKAGKEVTVAEPAPDATKVTDLMAALQASVERARSGRSAAATDKDKGSKAKGKKAPAEKAPAKKPATKAKKATAKKAPAKKAPAKKAEAPAKKTAAKRATKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W100</accession>
    <name>U5W100_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerophosphoryl diester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361733.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16760"/>
    <dbReference type="EnsemblBacteria" id="AGZ41630">
        <property type="protein sequence ID" value="AGZ41630"/>
        <property type="gene ID" value="AFR_16760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3398"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074029_0_0_11"/>
    <dbReference type="OrthoDB" id="1542724at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665571" evidence="1">
        <location>
            <begin position="30"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="2">
        <location>
            <begin position="38"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="325" mass="35821" checksum="E7BEE2281137F2E2" modified="2014-01-22" version="1">MNRRRRRLLITLGVVLLTTAGLFTANSSALWGDDDGRPLLLAHRGIAQTFPLDGVDNNTCTAARIHPPDHAYLENTLASMRAAFEAGADMLEFDVQVTADDRLVVFHDSTLECRTDGSGRIRDHTLAELRRLDLGHGYTADGGATYPLRGKGSGLLVTVEEVVAAFPDRELLIDLKNDEADEGERLAAYLATLPAERLATIGVYGGDAPVEVVRSRLPQVRATSRKIMKSCLTGYLALGWSGHVPGACRHTELHLPERYGRLMWGWPLLFADRMREADTRVILVAGDGPWSAGFDRPEDLETMPAGWSGWLWTNRTDRISPVLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W147</accession>
    <name>U5W147_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_22335</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363108.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22335"/>
    <dbReference type="EnsemblBacteria" id="AGZ42737">
        <property type="protein sequence ID" value="AGZ42737"/>
        <property type="gene ID" value="AFR_22335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4528"/>
    <dbReference type="eggNOG" id="COG1053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_8_5_11"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="4">
        <location>
            <begin position="10"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="FAD_binding_2" evidence="3">
        <location>
            <begin position="103"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="518" mass="55043" checksum="342BEFBB4BCC81D4" modified="2014-01-22" version="1">MSDLDLDAGVLVIGGGLAGTWTALSAATAGASVVLVDKGYCGTSGVTATAGVSHWLVPPEDRAAAIAERREIAGGLAEDTWMARVLEETWDRLHTLDLPFPQARDGRRPYRTIRGPEYLRAMRRLVKRAGVRILDHHPALELLADADGRIRGAAGIRRQAGSGRWRIRAGAVVLATGGCAFRSRLLGAHGNTGDGLLMAAEAGAELSGMELSSYYTPAPAFSSVTRSMSYLFGTYIDGAGREIDLPAGPSRTEALARALLEGPVRVRFDRMPPAIRNRMRLVQPNLMIPFDRRGIDPYTDFFEVTLRGEGTVRGVGGLRITSGACTTTVPGLWVAGDAAAREPIAGAISGGGAQNSAWAVSTGTWAGAAAAAGAVPVTDRRLRPLGRTGMRPSTSTSALDAREVALAVKDEMNPYDKNLFRTGTGLRTSLAVLDRLWSASADGLRGDDPVAAREAAALVAAGRWAYTSALHRPETRGLHRRTDRPWTDPALTARLHTGGLDRIWHRFVPVPVSAEVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W162</accession>
    <name>U5W162_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Signaling protein ykoW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_17085</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361865.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17085"/>
    <dbReference type="EnsemblBacteria" id="AGZ41695">
        <property type="protein sequence ID" value="AGZ41695"/>
        <property type="gene ID" value="AFR_17085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3466"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="458"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="530"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="5">
        <location>
            <begin position="613"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="4">
        <location>
            <begin position="759"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1018" mass="109730" checksum="7B24C584404D533C" modified="2014-01-22" version="1">MLLRTAHARFVIGMLVLTAAFYALPEYRMYTWAAIGLSAAAAVLVGVRKHKPSRRLPWWLLAGVLVSFTAGDTTYNILTDHLHQENPFPCLADGFYLASYPLLAAALLIFIRARSGSGNRAALLDALVPTAGLGLLSWVFLIAPYVRDTDLTIMEKLTSVSYPLGDVLALAMLLRLLIAPGRKPLAITVLGAGVSGLLVTDVLYGLRQLAGTWATGGPTDAGWVFFYAAIGVCALHPSMTQLTLDRQTAPVAVHASGRRIALMSFAALIAPVMLLVEDLRNEVHDALVIAVACASMFLLVIARVVDLLRAQREAGARERALREAGALLVAASTEKDAAAALRQAVDDLVPDGEPYRLHFVAEDFFGDTPPPHLAVMVEVASLPESLAAEFAGFEFALHATVLGSGRTATSPARSKQTYLAAAPGRLHSLRPSFDALMAQGAMAIGRIGLTDEVSRRSSEDYFRTLVQSASDVILIVGGDEEIRYASPSAEHVFGRPELVGSPLTGLFAGTDHAELRDLLSRAHLGRGRQDGIDLTAIRADGRLLQVESGCRDLRDDPTVNGYVLTMRDVTERRQLENDLTHQAFHDGLTGLANRVLFQNRLEHAAVRAENDGSTIGVLFVDLDDFKEVNDTLGHAVGDQLLIAVGERITRAIGPLNTAARTGGDEFAVLVEHAAGPDDVDELAGLIVAALGVPVEVGDGAGGTHVVSGNASIGVATTAEAGSTSELQRQADLAMYLAKGEGKNTWQRYRGDLHSGVVERLELRAALNDAAAAGQFVLRYQPIVELGSEDVVGLEALVRWEHPTRGLLGPDQFIDLAEENGSIVAIGNAVLREALRAFVGWREAAPDRPMRYVSVNVSARQFRTPGFVAQVRDALAETGARPEWLLLEITESLVLRDAEKVWADLRELREWGVRIAIDDFGTGYSSLSYLRQMPVDVLKIDKSFIDDILHSDEQLALVEAIVSLARTLKLAVVAEGIEHRDHHDVLLRLGCPYGQGYLFSRPVPPDQVTAWLAPEAVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1E1</accession>
    <name>U5W1E1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATP-binding region ATPase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_23470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23470"/>
    <dbReference type="EnsemblBacteria" id="AGZ42964">
        <property type="protein sequence ID" value="AGZ42964"/>
        <property type="gene ID" value="AFR_23470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4756"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_3_11"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="119"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="36204" checksum="38358C3ABCD30319" modified="2014-01-22" version="1">MKDMLLIGLYALVSGAVIATGGAIALRLLRGRSILVHIGVLLAATVIAVVSGVVTVAQAMFLSAHDLQVVLITVAASAVVSLAVGLVFGRRLATSAVWAAQAEAQERKAEAGRRELVAWVSHDLRTPLAGLRAMAEALEDGVVHDRESVAEYHRRIRVETDRMTRLVDDLFELSRINAGALRLVPTMVPLADLVSDALTTVAPLAASRRIRLVAAGTGWPVVTASEPELARAVTNLLVNSVRYTPPDGTVHVAAGHDHQDVWLSVSDTCGGIPEADLPRLFDVAFRGERARTPAHGTDLSSAAGGGLGLAIVRGLVEAHGGRVEAHNIDRGCRFIMRIPAVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1R3</accession>
    <name>U5W1R3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363748.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24460"/>
    <dbReference type="EnsemblBacteria" id="AGZ43158">
        <property type="protein sequence ID" value="AGZ43158"/>
        <property type="gene ID" value="AFR_24460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4954"/>
    <dbReference type="eggNOG" id="ENOG502ZWW8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2115737_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="114" mass="12422" checksum="5E08C2B4A79ACD7C" modified="2014-01-22" version="1">MDGATAGTEPATISDTERAVYGSVTSLELRAFLDAWTTRRLGSPIEKVRFRAGRIDVVWGVELGDGRTVVIKTHRPPVDLDAARSPAKPSSCWPKQASLAQCRWPDRTRSKAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Z1</accession>
    <name>U5W1Z1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative magnesium/manganese-dependent protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363714.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24290"/>
    <dbReference type="EnsemblBacteria" id="AGZ43124">
        <property type="protein sequence ID" value="AGZ43124"/>
        <property type="gene ID" value="AFR_24290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4920"/>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043255_1_0_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="174"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="392" mass="41665" checksum="D60808DB9CDEE3D5" modified="2014-01-22" version="1">MTSEQEGLWLRALSSVLERSHLWLPEELAPAVAAAVAPLGIRPAIYVVDDEQRALRPVPADDRPAGGPLSLEGTVAGRAFRTVRTQPGGTASWWVPIVNGTDRLGVIEFAFPGGLDTGDEVLRRRCEVLAGLVGHLITVTVPKGDFLLQARRARPMSASAELLDRMLPPLTVSCERLTISAILEPRYDVGGDGYDYALDGSTAWMSVFDGVGHGLRAGLAYTVAVVAIRAARRAGAGLAAQVAAADAALLEQFGAEARFVTAVVAELNLDTGDLRYVNAGHPAPLVLRGGKFVEELPAGRRTPLGVPPVRGEIGHATLRPGDWLLFYTDGITEARNNEQQMFGTGRLIEHAERHAAAGLAAPETLRRLAHAVAEFHEGSAVDDATLLLAALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W265</accession>
    <name>U5W265_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">O-phthalyl amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24765"/>
    <dbReference type="EnsemblBacteria" id="AGZ43219">
        <property type="protein sequence ID" value="AGZ43219"/>
        <property type="gene ID" value="AFR_24765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5017"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042159_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665994" evidence="1">
        <location>
            <begin position="26"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="454" mass="48118" checksum="EA7323194920FD87" modified="2014-01-22" version="1">MDLKRTSILLVPALLLGLVTAPAAAAPVVCAAPVPSATQPGYTVADPACDLNGTPFTASASSRVYTGIRDGAAYRIEVPEAWNGQLVVFAHGYRGTGKVVSVDSPDLRAHYVAEGFAWAASSYQTNGYDVGQGVRDSYALIDRFRQVTGRSARSVVMTGKSMGGHITAVAIEEHPRAFTGAMPYCGVLGDTELFDYFLDAGVTAAALAGVEPSFPVEPGPWQAEVDRIVPALGLKGTLTGAGRTWSSVVERRSGGVRPGFAGAFSYWNAAPASDPALKMPFLFGVYPGLTGGTAGIADGNVTDNRFAFYRSTDGWPTAAEWQLNARVKRVRATDRASAGLDGIPRVDGRPRVPVLSLHDLGDLFVPFSMEQIYAQRAARNGRAQLFVSRAIRGVGHCDFTQPELQQGFDDLMTWVRTGRRPGGDAILDRRAVAAPGFGCRWTVGTRAAFGPACG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W289</accession>
    <name>U5W289_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Manganese/zinc/iron transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362409.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18800"/>
    <dbReference type="EnsemblBacteria" id="AGZ42036">
        <property type="protein sequence ID" value="AGZ42036"/>
        <property type="gene ID" value="AFR_18800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3819"/>
    <dbReference type="eggNOG" id="COG1121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_11"/>
    <dbReference type="OrthoDB" id="1721927at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="25749" checksum="F6C5F902FC0A21D4" modified="2014-01-22" version="1">MAEGDVALRYVTADLGYRGRAVLRGVDFTVRDGETLALVGPNGAGKSTLIKAVLGMAEVVSGEVVARAPVGYVPQIDSLDADFPVSAADVVLMGRYRRIGWWRPTGRADRAAVRDALDRVGLADRARVRFGLLSGGQRQRVLVARAIAAEARLLLLDEPLNGVDATSQEAILRALGELTASGAALVVSTHDLNVAARIADQVCLLNGRQWAVGAPADVLTGDVLRQAYGGHAIDLPGGRALLVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2D0</accession>
    <name>U5W2D0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cellulose synthesis regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362429.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18910"/>
    <dbReference type="EnsemblBacteria" id="AGZ42056">
        <property type="protein sequence ID" value="AGZ42056"/>
        <property type="gene ID" value="AFR_18910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3841"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022176_3_0_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="389"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="525" mass="56460" checksum="87A548B3C2B275FD" modified="2014-01-22" version="1">MTTTHAPADARRLADELLTLEIGFAATVQDDRIAAGRIQREAVALGRTDLVLRARVAEIVVLLREGDTAGGGRAIHQVHPEAVELGDDYLLARCHRALSIFFRQLGDLATALAHAVQCMSHTADDVVPAIRARHLMCLAVLLDETGSPGEALRRFGEALEIATVHRDAELALLLLNNMTYTACVRGDLAAAEDLERRMRFAARSAGVPLSATCLDTCARVAMLAGRYADVEVILGAFLTDDAPKHLLDEGNALEEAMLTAAEAQRELGNDSRAQAILDRVLRLCAEHDLPGLQARAREQQALVHAAAGRYREAFEEYRRFHTETQVLTSAQQDARSRTLQAVHQAEEARRATDEFRELAHRDALTGLHNRRHVDEALPVALGVIGVRGGWLSVALIDLDHFKRVNDTRSHETGDLVLRDVADLLLGTAPDDAVVGRLGGEEFVVLLPGADATAAWIAAERIRLAVAGHDWAPVTGGLPVTCSVGVTSVRGPGPAPAAVLAAADRNLYAAKRGGRDRVCGDPVTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2V5</accession>
    <name>U5W2V5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25415"/>
    <dbReference type="EnsemblBacteria" id="AGZ43347">
        <property type="protein sequence ID" value="AGZ43347"/>
        <property type="gene ID" value="AFR_25415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5149"/>
    <dbReference type="eggNOG" id="COG2501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127162_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="65" mass="6905" checksum="A059005EE400BB1F" modified="2014-01-22" version="1">MAIDSDMIRLGQFLKLADLIDTGGEAKVLISVGDVSVNGEVDTRRGRQLHPGDVVTVRGQSARVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2X9</accession>
    <name>U5W2X9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AP_endonuc_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362722.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20390"/>
    <dbReference type="EnsemblBacteria" id="AGZ42350">
        <property type="protein sequence ID" value="AGZ42350"/>
        <property type="gene ID" value="AFR_20390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4134"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059523_1_0_11"/>
    <dbReference type="OrthoDB" id="1194699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="AP_endonuc_2" evidence="2">
        <location>
            <begin position="93"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="40776" checksum="11C0A5DE108C594D" modified="2014-01-22" version="1">MDKISDGTPLDAATRRRLTRRGILMASAGAAAALGAAGMNVLQTDAPAVAGRELHPEPLIPGRNRGIILYSVRDRIAAAPDSSGVPYGFERVLARLAELGYKEIEFAGYNQSTEILGRQITPAEIRKILDDNGLVANGTHAQINAATFEQQMDIAQTLGMRNIGTGSDPANSSYTADWDAAADLWNDLGRRARARGLRLYTHNHDTAYSFLLDSGPLDAAGKPTRSSGTRRLEYFFRKTDPRYVFFELDIYWAYVARFKHQKYVDRGGVERTDLFDPILNVTSRSKRFPLFHAKDGDRDSAQANGYVMTPLGEGDINFQQFFATIGDPEYRHANWEQDTAPGGAANPAQSLDFAALSYRNMADLTIYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3L7</accession>
    <name>U5W3L7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560150.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27760"/>
    <dbReference type="EnsemblBacteria" id="AGZ43813">
        <property type="protein sequence ID" value="AGZ43813"/>
        <property type="gene ID" value="AFR_27760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5626"/>
    <dbReference type="eggNOG" id="ENOG5033K9A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_198268_0_0_11"/>
    <dbReference type="OrthoDB" id="2022855at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="66" mass="7344" checksum="8D5304F6224D61A4" modified="2014-01-22" version="1">MSDALEHLRRWEESGATWRVVVRTPESVELALLTCDAGEEVDRLRSADPAVLAHIGDRSRSEDDAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3S5</accession>
    <name>U5W3S5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363001.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21800"/>
    <dbReference type="EnsemblBacteria" id="AGZ42630">
        <property type="protein sequence ID" value="AGZ42630"/>
        <property type="gene ID" value="AFR_21800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4417"/>
    <dbReference type="eggNOG" id="COG1607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149200_0_0_11"/>
    <dbReference type="OrthoDB" id="1712258at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="12636" checksum="31442AC03BAA7FAF" modified="2014-01-22" version="1">MITVTHRRYVPYSHAHYAGNLVDGAYSLGLFGDVATEVCIRLDGDEGLFASYSDVQFKAPVQAGDVLEVTATVTRMGTRSRQIAFTSAVVCRGTGGSAASVLDEPIIAVTATGTVVVPAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Z9</accession>
    <name>U5W3Z9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560196.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27990"/>
    <dbReference type="EnsemblBacteria" id="AGZ43859">
        <property type="protein sequence ID" value="AGZ43859"/>
        <property type="gene ID" value="AFR_27990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5674"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_11"/>
    <dbReference type="OrthoDB" id="961884at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="16"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="377"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="457" mass="48172" checksum="9EAA5137DC488BBD" modified="2014-01-22" version="1">MRLIAGLTRPSPTDGAAIRFGTQTLSGEQVLAAATAVADEVAGAQVVAVHATATAETVVAMIGALLGGAAVVPLPPDSGETETAHILNDSGAQVLLAHGEHPPTSVRTVPIDLRRTSATTYPAPPPEATALIMYTSGTTGAPKGAVLSHRAVEAGLDALAEAWAWHPDDLLVHGLPLFHVHGLVLGVFGALRVGNRLHHTVRPAPQAYAEAGGSLYFGVPTVWSRMCARPDLAARLAKARLLVSGSAALPVPVFERMRELTGHRPVERYGMTETLITVSNRADDPDRRPGQVGTPVAGVATRVVDEDRDVPADGESIGELWVRGPMLFDGYLHRPAATGWFRTGDIASVAPDGRHRIVGRRSTDLIKSGGYRIGAGEVEDALLAHPGVREAAVIGTPHDDLGEQVTAYVVGDAVSADELIGFVARTLSAHKRPREIRFVAELPRNAMGKVQKRLLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W429</accession>
    <name>U5W429_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_27580</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27580"/>
    <dbReference type="EnsemblBacteria" id="AGZ43777">
        <property type="protein sequence ID" value="AGZ43777"/>
        <property type="gene ID" value="AFR_27580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5590"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="52"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="254" mass="27243" checksum="73989A5EEDE3580E" modified="2014-01-22" version="1">MKLLWRVAPPVLLGIAALVAWELVVSLGRIAPFILPAPSAIWHELVAQRTNVWEAALASGLNALIGLAWGTVAGILAAMAASRFRLLSEVSVPLAAVLNALPIIALAPILNNMFESTSSVPRRLIAGIIVFFPVFINTLRGLREVDPTHRELMTSYAAGGWTFTRLVRLPGALPFVFTGLRQASSLAVIAAVVAEYFGGLQNGLGSRITSAAAFTAYPRAWAFVVGACLLGLVFYLSTLLLERLAMPWKTRLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4G0</accession>
    <name>U5W4G0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_22705</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22705"/>
    <dbReference type="EnsemblBacteria" id="AGZ42811">
        <property type="protein sequence ID" value="AGZ42811"/>
        <property type="gene ID" value="AFR_22705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4602"/>
    <dbReference type="eggNOG" id="COG1652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_564890_0_0_11"/>
    <dbReference type="OrthoDB" id="1790141at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665680" evidence="3">
        <location>
            <begin position="27"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="481" mass="50552" checksum="34C337A5CDFF9500" modified="2014-01-22" version="1">MVTPMVLRSLLAALALALAAATPVSPATPVAPVAFHRTEQGRTEQGRTEQRWAEQRWAEQRRAEPGDVKWYRVRPSFDGEEEYLYEIAERFLGDGDRHDEIFQLNKGRVQADGESLTVPDEIESGWVLQLPDDATGPGVEVGPLPTAPPSVSAAPLASPPAVGAAPEAAADEDSGLLLPLLGGGILVLAGGALLAFLLVRRRRVPPPTPPAFIPRQRTGPPPLRPLDPAAAWTVDRALRVLVTSAEAAGRPVPPVLGVTIDDSWVSLRLSAPDEQPFAPWEARSQGRVWIAPLRGLQALPADPATGTPCPRLVTLGTAAGTRELLDLGRAPGPIAVRGDTTAARALLAAWAVELTNSPWSAGVRVVAGGLRPEIEDEPRVVSAETPAAALAEILGPAPDPVAEPDDTEPGTAEPDTAEPGLADGWLGVLILSALPDRLPELPDGWTVVVLGRARQDGWQITVGPDGTVDTGRLGISIHAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4U0</accession>
    <name>U5W4U0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FBP_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560471.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29365"/>
    <dbReference type="EnsemblBacteria" id="AGZ44134">
        <property type="protein sequence ID" value="AGZ44134"/>
        <property type="gene ID" value="AFR_29365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5951"/>
    <dbReference type="eggNOG" id="ENOG5031HHY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116295_0_0_11"/>
    <dbReference type="OrthoDB" id="1684141at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032330">
        <property type="entry name" value="EF-G-binding_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16571">
        <property type="entry name" value="FBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBP_C" evidence="1">
        <location>
            <begin position="8"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="164" mass="17562" checksum="97308B3DDE701A92" modified="2014-01-22" version="1">MEPITEREIRASFVNCTKGATKRLAVPADLDQRPWGDLDFLGWRDPASLQRAYLVAESGNGLVGVAMRLASPSAGQPRRSMCSLCLTTHNPGGVSLMTAAKAGRNGQNGNSVGTYICVDLACSLYLRGKKKAGPATRFQESLTEDEKIERTVGKVEAFLASVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Y2</accession>
    <name>U5W4Y2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Subtilisin-like protein serine protease-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30065"/>
    <dbReference type="EnsemblBacteria" id="AGZ44273">
        <property type="protein sequence ID" value="AGZ44273"/>
        <property type="gene ID" value="AFR_30065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6090"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_536018_0_0_11"/>
    <dbReference type="OrthoDB" id="1382378at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666358" evidence="1">
        <location>
            <begin position="23"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="509" mass="54724" checksum="EFA8B79196846B78" modified="2014-01-22" version="1">MLLAAVALVPATIWTTTASASAASGSLTVTTYDRTGAKVSTPLSLTNIATNTDYTATSGKAKSLPKGTYTVVAAITTRRDSSDTVGARVLKVGSGKTTTTIDARAGKALKMSLDKPPAGQNQELRARICGGDTFSSQVEVYNSPGKIFVIPNKSKHLRSAFAASWSGQGSESWIVTSSSTTVPSSLTKSFKRSSLANLTAWARNGPSAGLETSIMLQAGGDQCRTDLSFGIYQENTPFTFKTHVSPGSWLVRADTWGRLNNGESTTLGFQWAQRTFTAGKSYTQTFFRSAWGPAYNVPRVSGGHLSFDTTSMFVDPSFGSQYYPQSEASEKSLVTLSLKGKVLKKQSRTDWGSIDTAWFEKKITKKGWYLLTVSAQRYRPGLKYPSGLLSDRTGVWFSFYADPKKNKTASVLLPRMNPAGLDIWNRAKPGSSTTVDIKLQGKAIPKSVTAKASFDGGKTWKSVPVKKSGSAWKATVKNPGSGVVALKTQTQDKGGNKTEVTIYRAYRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W526</accession>
    <name>U5W526_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MbtH-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560444.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29230"/>
    <dbReference type="EnsemblBacteria" id="AGZ44107">
        <property type="protein sequence ID" value="AGZ44107"/>
        <property type="gene ID" value="AFR_29230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5923"/>
    <dbReference type="eggNOG" id="COG3251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181321_1_0_11"/>
    <dbReference type="OrthoDB" id="2067093at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005153">
        <property type="entry name" value="MbtH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038020">
        <property type="entry name" value="MbtH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037407">
        <property type="entry name" value="MLP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38444">
        <property type="entry name" value="PTHR38444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03621">
        <property type="entry name" value="MbtH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00923">
        <property type="entry name" value="MbtH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160582">
        <property type="entry name" value="SSF160582"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MbtH" evidence="1">
        <location>
            <begin position="3"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="73" mass="8383" checksum="8C45FE70300F265B" modified="2014-01-22" version="1">MSNPFEREDISYFVLVNDEGQHSLWPEVIDVPAGWRTVSGPESREDCLAYIERSWWDIRPASLITRMASAQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W586</accession>
    <name>U5W586_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363730.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24370"/>
    <dbReference type="EnsemblBacteria" id="AGZ43140">
        <property type="protein sequence ID" value="AGZ43140"/>
        <property type="gene ID" value="AFR_24370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4937"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_11"/>
    <dbReference type="OrthoDB" id="831053at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="10"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="36019" checksum="E1F794B0946D0286" modified="2014-01-22" version="1">MTVEVVLPGKFPQALEVREREIGEPAAGQARVRVDATGISYAEQAMRRDLYPGQPSFPFTPGYDFVGTVEAVGPGADGTLVGSRVAAVVKTGGWASHVLIDALDLVRVPAALDPAEVETVVVNGVTAWQMLHRSARVSPGQTVLVHGANGGVGMILVQLALHHGVHVIGTASPRHHDALRALGVTPLDYNDPDLAARVRDLAPAGVDAVFDPIGGASVRTSYSLLSRGGVLVAYGNASALGGKQPMELVFVRLLARLFVWNTLPNGHRALFYNFWAGKLVRRATFRRRLREDLTTVIGLLESGAVVPEIAARFPLSEITAAMELAESRTVRGKVILEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5D4</accession>
    <name>U5W5D4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_30495</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560696.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30495"/>
    <dbReference type="EnsemblBacteria" id="AGZ44359">
        <property type="protein sequence ID" value="AGZ44359"/>
        <property type="gene ID" value="AFR_30495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6174"/>
    <dbReference type="eggNOG" id="COG1126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1191583at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="26930" checksum="AD5FE018E6D7C939" modified="2014-01-22" version="1">MLLSCENVRKTFGTNVVLDDLSLEVGEHQVIALIGASGSGKSTLLRCVNLLTGIDDGTIRLDGEDITDPRVNPDVVRRRIGMVFQSYNLFPHMTVLKNITLAPARVHKRAAAEIRDQAMEWLTRVGLADKADSYPDRLSGGQQQRVAIVRALVNNPRLLLLDEVTSALDPELVGEVLTMIRGLKDEGMTMVLATHEMGFARQVADQVVFLDGGRVLEAGPPAQVLGEPTEARTRQFLARIIEAGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W663</accession>
    <name>U5W663_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative HxlR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25885"/>
    <dbReference type="EnsemblBacteria" id="AGZ43441">
        <property type="protein sequence ID" value="AGZ43441"/>
        <property type="gene ID" value="AFR_25885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5247"/>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_2_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="16002" checksum="69173466AC15C6BF" modified="2014-01-22" version="1">MSKHPILARAAETDAFVGDCPTREILERLGSKWVSLIIVALADRPHYFGELLKRIEGVSKKMLVQSLRMLESYGLIGRVSVPAGAVVKVRYELTEQGQSLVEPLFAVYVWAQRNTDAMLSNQESYRRKDDGGNGDRVRVSAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W699</accession>
    <name>U5W699_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_31945</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560982.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31945"/>
    <dbReference type="EnsemblBacteria" id="AGZ44649">
        <property type="protein sequence ID" value="AGZ44649"/>
        <property type="gene ID" value="AFR_31945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6466"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_0_11"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="100"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="35657" checksum="4F8F6EB50A8A4EBE" modified="2014-01-22" version="1">MKFLVQRVLFYLFTAWAAITINFFIPRMIPGDPVKSLLARFQGQMSTDAIDSLYVLFGLDKNASVWSQYIDYWKQLFQGDLGLSFTAFPSPVSEVIGDALPWTVALVGITTIISFFIGTSLGVLAGWRRGSWIDGLLPATTFLSSVPYFWLGLLAIYLLAGPDSFFPSSGGYDGAYVPAWDQYFIPSAIQHSLLPALTILISSVSGWILSMRNMMVTVAAEDYITVAHAKGLSDRRVALSYAARNALLPNVSGFALSLGFIVGGTLLVEIVFSYPGLGFLLFRAVGAKDYPLMQGIFLVITISVLVANLIADIAYLLLDPRTRKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6D7</accession>
    <name>U5W6D7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364303.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26295"/>
    <dbReference type="EnsemblBacteria" id="AGZ43521">
        <property type="protein sequence ID" value="AGZ43521"/>
        <property type="gene ID" value="AFR_26295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5330"/>
    <dbReference type="eggNOG" id="COG4977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_59_0_11"/>
    <dbReference type="OrthoDB" id="1459004at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="210"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="33016" checksum="9BF5A3739312D5EE" modified="2014-01-22" version="1">MPSVALAVTDGTPLFELAAACEVFGVDRGLAEPWYDFTVCGDDGAAVGGWLTAGIRHGLDALADADTVIVPSCRDAEETPPPTLVAAVRAAHHAGARIASLCTGAFVLAEAGLLDGRRATTHWAHTTLLGDRYPAVSVDPDVLYIDDGDVLTSAGKAAGLDLCLHLVRTDHGTAVANALARSLVVPPHRQGGQAQYIPAAVSHGPDHALAGLLPWALEHVAEPLTVTDLARRAGMSTRNLARHFHAVTGVSPLRWLLTQRVRLAQELLETTDLSIEQLASRTGLGTATTLRRHFKEQLGVPPETYRRTFRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Z7</accession>
    <name>U5W6Z7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_32595</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32595"/>
    <dbReference type="EnsemblBacteria" id="AGZ44777">
        <property type="protein sequence ID" value="AGZ44777"/>
        <property type="gene ID" value="AFR_32595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6596"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003050_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="LamGL" evidence="3">
        <location>
            <begin position="711"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="LamGL" evidence="3">
        <location>
            <begin position="984"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="652"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="654"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1152" mass="122319" checksum="E43CAC8CCEEA49EC" modified="2014-01-22" version="1">MSERTEYSQFFAEPGGNLTYESTVLPQRVHRSDGSWADVDLTLQQSAEGTLRPQSSVADVRFSSGGEGPLVTLVDHGKSFTMTWPGRDLPAPTVTADTATYAEILPGVDLVVRATSLGFSHVLTVKTADAAKNSALREITFDLGGAAEVSRLPDGSLQAAAGSSLLASAPAPAMWDSRSTGATAKTSAESAAAVQGAGPDISTAAAPGDAARVVSVETEVNSAGDLVLKPDADMLAGPATFPLFIDPEWSTGKKRWAYATNNNTSNGDVSRARVGMDPDGRIYRSYFEFSLAALKGKHVESAKVKMEVDHSWACTPTWTHMYSANPISSTPRTAWKSSNPFLKHLSAAESHANEGVGCEDSPQPDSPVTFTGGAIGSLVNTLAGRSADTVTMAFSAGNDNQEYENAKDRWKKFFLGSAKLIADVDAKPGKPYSLQVNGVACQSASVGVGLTNPYFGAFMPDSDTSQTLRSTWEWYKVVNGAISTKMTSPAVSQTPANSRDASVRASGAVNNQTYAFRVQGKDPAPYEITSPWSDWCYFHIDTTDPPVVGTVLSMPQGPGKPGQFEISSTAADVTKFRYGWNAAGNEIAAKTVPNRPGKSVIVTLSAPKYGENVLYLQAVDSTKNVGDGSLSIIVQRPSPPVARWGLESYPGVSTDDALSDQQGELAGDTPLSASTSGFSWSDRQRMVGTSTATFTGTGVLTTTTPVLDTARNYGVAAWVKLDQMTGGQNIVSQDGNQTANFQLQYRSDDRNGDGIADKSFCFTLRNEDVAATAALTTVCGVNTAVAGRWTHVAGGYDAAEKKIRVWIDGVMKGEADAPQMWSAAGPLRIGDRKLTPTQWTDQFFGSVADVQVFDRVLVQDDFTGRIAEPGSQMLDEPGILTPIEVGRWDFAVASECYDAAIPDTCAAADAAPWGRRLRLTPGTEIGDGSGGGFAAFDNQHLWWVGPDDPVYGTATREYGFSQQNTAGPEDEPQWQDSPVVRTDQSFTVSVMTHVDSIDTTMTAIAPKGGKQSAFYLGSRKSTVGSVSAQRFEVMVPSLDQDLGESYTHIVAPTPIDIDDAGSWTQLTVTYVAGTKKLQLYVNGKLAQSGSVPGLWNAGGPLIVGNSWWSADNTSGGWTDAWFGGLDDVRVYQGAMTAAQVAELYASQSVDAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7A3</accession>
    <name>U5W7A3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561361.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33830"/>
    <dbReference type="EnsemblBacteria" id="AGZ45024">
        <property type="protein sequence ID" value="AGZ45024"/>
        <property type="gene ID" value="AFR_33830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6848"/>
    <dbReference type="eggNOG" id="COG3903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243470_0_0_11"/>
    <dbReference type="OrthoDB" id="1789110at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="26103" checksum="874809C00D72B45E" modified="2014-01-22" version="1">MGTTGETLAAGNEAMLEAAFRTGDYDQAERAFQELLSQARADGDRALEAAVLDRLGMLMHFQALEGDLQQADSEGEEALFQQALEIRRELGDPAGTAESLFGVGLVHQVLRQDWDAAMPYYWEALDLAERYGDLLLRSEVHRHVGFYYAMSDLQSDKALDHLRISHDLRAELGDDRWIPGGTVALGIAELADGLREQGLAHVRQAVAEAHAAGLSQQRIEWMEQFLQKAEAGDVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7D5</accession>
    <name>U5W7D5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561425.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34150"/>
    <dbReference type="EnsemblBacteria" id="AGZ45088">
        <property type="protein sequence ID" value="AGZ45088"/>
        <property type="gene ID" value="AFR_34150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6910"/>
    <dbReference type="HOGENOM" id="CLU_1369667_0_0_11"/>
    <dbReference type="OrthoDB" id="2201588at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="199" mass="20679" checksum="50C608997D7AF512" modified="2014-01-22" version="1">MKFSLRGPRTAAGQVVAFLGCYVAAALIAALMVFVHLPGDGAVIVVSFAVVGALALGHLLTRYRLWLQVCAAFTGATFVIVTGYAAHSTVLAVHGDRISAVVAGVAVQERSGNSTTYRYALTTLLGVPVPGQLVEDSDELAIGEQVEVVVDRGGRVDPRTTGEVDAVGPLWAAALIGFALTALVAVLAGPVTLRRQRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7R7</accession>
    <name>U5W7R7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28675</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560333.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28675"/>
    <dbReference type="EnsemblBacteria" id="AGZ43996">
        <property type="protein sequence ID" value="AGZ43996"/>
        <property type="gene ID" value="AFR_28675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5813"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_0_11"/>
    <dbReference type="OrthoDB" id="1875458at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="238" mass="26294" checksum="5AC3570FB92CB545" modified="2014-01-22" version="1">MPSVAERIAAELEEEILEQRLGAGARLALRTELIDRFGVSANAMNEALRILRERSIIEVKPGVQGGVFVAQPPPQLRLGIIDVWFRGLLVDAVDLFEARTMLEDSFAAVAAERATPEDCRDLDWAVESLRDSRADPRGYLEANIRFHRTIARAARVPVLAGMYESLVTLIRATLVRAEFAGPDAAKVIDENITVHARIATAIRHRDHEALSAAILAHRTDLVRLTDPSRSPGRTRPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7W6</accession>
    <name>U5W7W6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560392.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28970"/>
    <dbReference type="EnsemblBacteria" id="AGZ44055">
        <property type="protein sequence ID" value="AGZ44055"/>
        <property type="gene ID" value="AFR_28970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5870"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_12_3_11"/>
    <dbReference type="OrthoDB" id="1282004at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="25"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="30432" checksum="FB001EDE8C4B481D" modified="2014-01-22" version="1">MSTISVGTENTTSIDLYYEDHGSGPAVVLISGWPFDSRSWESQIHPLLTAGHRVIVYDRRGFGRSSRPVGGYDFDTLAADLDKLLTALDLREVTLVGLSLGTGEVARYIGVHGTGRLRSCVFIESLAPSFAKSAENPNGVDQAGVDGVQQAILHDRPAWLTGLLGDMLNLDDLLGSRVSEEAVRNLWNAGADASPEATWACPQTWLDDFHDDIKRVDVPALVLHGTADRILSIDGQGRRMHAALPDAHYVEIEGGPHLMCLSHADEVNRELLAFLATVAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8B8</accession>
    <name>U5W8B8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35665"/>
    <dbReference type="EnsemblBacteria" id="AGZ45389">
        <property type="protein sequence ID" value="AGZ45389"/>
        <property type="gene ID" value="AFR_35665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7218"/>
    <dbReference type="eggNOG" id="COG3393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085660_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013653">
        <property type="entry name" value="FR47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08445">
        <property type="entry name" value="FR47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="35"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="18796" checksum="39F0A32FEFCA5F54" modified="2014-01-22" version="1">MSISARPPEDAGWEIRGTSTAFQLIDGKVPAALDSEAIVLTVADVPDMIDLVSRTEPGPFRPRTIELGTYLGIRQHGRLVAMAGERLHPPGWTEISAVCTDPAHRGRGYAARLVRALAAAIYDRGERPFLHTMATNTPANRLFTSLGFVRRKENTTVSLRVPVKKERLPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8C1</accession>
    <name>U5W8C1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44171">
        <property type="protein sequence ID" value="AGZ44171"/>
        <property type="gene ID" value="AFR_29550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29550"/>
    <dbReference type="HOGENOM" id="CLU_1718413_0_0_11"/>
    <dbReference type="OrthoDB" id="2057974at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="16410" checksum="609BE9C4C1B0FB3D" modified="2014-01-22" version="1">METLTDIAYTGRADLDSLTVAIREVFEPGAGAIRKAIGHPAAPLLAESLIVLIRARLEAIARLAEGVVTEDELRRARHAHLVAYAEYAIQQPSYTAHAPSAMPDMYEPVTAETMLVNSCGHLLTILGINALNPQRTVGFAAVPAPRIVFEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8H0</accession>
    <name>U5W8H0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560612.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30075"/>
    <dbReference type="EnsemblBacteria" id="AGZ44275">
        <property type="protein sequence ID" value="AGZ44275"/>
        <property type="gene ID" value="AFR_30075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6092"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155949_0_0_11"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="13345" checksum="39B7F93CA441F1C6" modified="2014-01-22" version="1">MSGKVPAARQELITGFYAAFNRRDLDALLGALTADVVWPDGSKGGSLSGTDEVRDHWTRQWAQSNPSFVPTGFRPEKDGRVAVLVRQVVKDKAGATLSEGQVIHVYRFAEDLVSEMEIREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB13</accession>
    <name>U5WB13_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561458.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34315"/>
    <dbReference type="EnsemblBacteria" id="AGZ45121">
        <property type="protein sequence ID" value="AGZ45121"/>
        <property type="gene ID" value="AFR_34315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6943"/>
    <dbReference type="eggNOG" id="COG4947">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090325_0_0_11"/>
    <dbReference type="OrthoDB" id="1828423at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="27528" checksum="7DB89FF2FE17DC84" modified="2014-01-22" version="1">MRHYSVELGSPSGGASGTVAVYGHWGRPVLVFPTELGRAWEFADNGMVAAVEDLIEAGRIKLYCVDSFDAATWSADYLPLEERARRHASYESWINDAVVPHIRADTGTAAEIMTTGCSLGAFHALNFAFKRADLFPLALCFSGNYEPAAWRSWGERGDAVYFNSPTDYIANMDGDHLEWLRSRLSVLLVCGQGQWEDTTGALESTRRMSQLLAAKGLRHDLDLWGPDVPHDWPSWRAQLAHHLPRFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBG5</accession>
    <name>U5WBG5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Flagellar hook-associated protein 1</fullName>
            <shortName evidence="7">HAP1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">flgK</name>
        <name evidence="10" type="ORF">AFR_40390</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Bacterial flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562664.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40390"/>
    <dbReference type="EnsemblBacteria" id="AGZ46332">
        <property type="protein sequence ID" value="AGZ46332"/>
        <property type="gene ID" value="AFR_40390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8175"/>
    <dbReference type="eggNOG" id="COG1256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012762_1_0_11"/>
    <dbReference type="OrthoDB" id="1815809at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002371">
        <property type="entry name" value="FlgK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30033">
        <property type="entry name" value="PTHR30033"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01005">
        <property type="entry name" value="FLGHOOKAP1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02492">
        <property type="entry name" value="flgK_ends"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="8">
        <location>
            <begin position="8"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="9">
        <location>
            <begin position="403"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ46332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="460" mass="48416" checksum="4396A68CFC3FCFBA" modified="2014-01-22" version="1">MASSFGGINTALTSLYAQRRGLDVTGQNIANANTEGYTRQRVEMQSQVGSISPAMYAKTDGLGTGVAVSDVQRLRDEYLENRGRSEHGASAYLASQSASYTTIEDAFAEPSNTALAAQLRDMWGGWNDVANNPQLTAPRSALIQQSATVVNQLNGAHEGLARQWSQNRTQMDTYVDEINTAAGSIAQLNESIIHANATGLTVNELEDRRDLLAMRLAELTGASAAKRENGGMDVFIGGATLVTGSVTRKVEFTGAARLEDQNADPVALRWKDTGGATSPGGTLGAMTDTMSIIIPGLSASLDGIAKKLADSVNALHANGFSTDGTTGLDFFGGDSAATIKVLVTDPDKVAVSAVSGTRDNSVADAIADIGDAAGGPDRQYQEMIGQLGVQSQGSARRAEIQSVVTEQVDAAREAQSGVNLDEEMTNLLTYQRGYEAASRVLTTIDSMLDQLINRTGLVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBJ4</accession>
    <name>U5WBJ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycerophosphoryl diester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561701.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35545"/>
    <dbReference type="EnsemblBacteria" id="AGZ45365">
        <property type="protein sequence ID" value="AGZ45365"/>
        <property type="gene ID" value="AFR_35545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7194"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_3_4_11"/>
    <dbReference type="OrthoDB" id="1542724at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="4"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="27667" checksum="5864312D03F1FCF5" modified="2014-01-22" version="1">MAGLHNVAHRGYSAVAPENTLPALAAGVLSGATFIEFDVRTTADGVPVVIHDRTVDRTTDGTGKVWELTLDEVSALDAGSWFSPAYAGTRVPRLAEVLELLRPHAVLTPTADVTSFERPRVELLLEIKPPATLEQAKIIMGQVVDAGLLDRTVVQSFDPNIVRLVAEAAPEVRRGLLRDGFDTSQVDVARELGIVYCNPSMNAVIGSPETVAQLAEAGVAVMPWTANDVSLWPDLLKTGVVGLISDRTGELTGWAAARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBL1</accession>
    <name>U5WBL1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40640"/>
    <dbReference type="EnsemblBacteria" id="AGZ46382">
        <property type="protein sequence ID" value="AGZ46382"/>
        <property type="gene ID" value="AFR_40640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8225"/>
    <dbReference type="eggNOG" id="COG2981">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070331_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="270" mass="28076" checksum="4EB6AE7DEA31D411" modified="2014-01-22" version="1">MGDNSTQLLSATGGAFRQFVTGAGLLARGLGLVLRNPRMLGLGLLPALISGVLYAAALIALIAFIDDLAAAVTWFADDWSTAGRDVMRLLAGAAVFGLCGLLGVLTFTAVTLLIGDPFYEKISGMVEHRFGGVRDEVEVSLGQSLARSLVDSLRLIGLSVLFGIPLFLAGFIPVVGQTVVPVIGGAVGGWILAVELTGVPFQRRGRRLRDRRAALRVNRPLALGFGVAVFACFLIPLGAVLLMPAAVAGGTLLSRKALGLPTEITTVASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBX6</accession>
    <name>U5WBX6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41825"/>
    <dbReference type="EnsemblBacteria" id="AGZ46619">
        <property type="protein sequence ID" value="AGZ46619"/>
        <property type="gene ID" value="AFR_41825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8464"/>
    <dbReference type="eggNOG" id="ENOG5033WEA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1460719_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="174" mass="18879" checksum="3DFBEDF591F1FBF5" modified="2014-01-22" version="1">MIGTAEDVAWIAGRTTQGFSVTTAIPPVFEAYATLYPPDGVSLIAHERAVVATLVQQTPEQPWWLGYLDTGAHDVVFPSAPRVKLYWDWSYVLVQAGPEQALTWRTGHMRGEGTLPDLFFPADRSWLVSALWDDAWTDLGGPADLIAALQRDPAAGARPVGPGDDALPPGLTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBZ6</accession>
    <name>U5WBZ6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Robl_LC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562975.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41950"/>
    <dbReference type="EnsemblBacteria" id="AGZ46644">
        <property type="protein sequence ID" value="AGZ46644"/>
        <property type="gene ID" value="AFR_41950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8488"/>
    <dbReference type="eggNOG" id="COG2018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094585_0_0_11"/>
    <dbReference type="OrthoDB" id="1635683at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="1">
        <location>
            <begin position="28"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="16139" checksum="6A83D5371D395985" modified="2014-01-22" version="1">MTSPYSTDTGNGRDYAQSQLSAEAKTFNWLLDSFTSGTAGVVEAIAVSSDGLLMAMSAIKDRPNAERLAAVVSGLTSLSGGAASWYNLGSLNRVIIDMADGYLLVTAISAGSVLGVIADRTANLGTLAYEMTLFATRAGGALSPRLIAELKNAVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCL1</accession>
    <name>U5WCL1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_42265</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42265"/>
    <dbReference type="EnsemblBacteria" id="AGZ46707">
        <property type="protein sequence ID" value="AGZ46707"/>
        <property type="gene ID" value="AFR_42265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8556"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01110">
        <property type="entry name" value="HTH_SoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010211">
        <property type="entry name" value="Redox-sen_tscrpt-act_SoxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30204:SF0">
        <property type="entry name" value="PTHR30204:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01950">
        <property type="entry name" value="SoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="16010" checksum="A7863C58CD4DBABA" modified="2014-01-22" version="1">MELSVGELAARSGVAVSALHFFERQGLIASTRTAGNQRRYRRDTLRRVAFIRIAQRVGIPLKEVGAILALLPDNRTPTREDWARVSECWQAELDRRMRHLEQLRNDFTGCVGCGCMSIDRCPLINPNDKLGREGPGPRRLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCN7</accession>
    <name>U5WCN7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR-type regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562022.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37160"/>
    <dbReference type="EnsemblBacteria" id="AGZ45686">
        <property type="protein sequence ID" value="AGZ45686"/>
        <property type="gene ID" value="AFR_37160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7518"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_17_1_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="19931" checksum="5DC44F7BE26080FC" modified="2014-01-22" version="1">MAPTTSTAALRADSARVRERMVAAAREAFVSGDTDLPMNAIAKAAGVGVGTMYRHFPTRQALLESMAAESFEALVTQARVAAAEPDIATGLAGLLRAALQCQLRDPSLAAVLATPTYACEETLELSRELGGASMELLERARVAGVIRPDVTADDMRRLMCGVRHAVVSGDDDGTVADRYLTIMIAGLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDQ8</accession>
    <name>U5WDQ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562380.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38960"/>
    <dbReference type="EnsemblBacteria" id="AGZ46046">
        <property type="protein sequence ID" value="AGZ46046"/>
        <property type="gene ID" value="AFR_38960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7885"/>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="12"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="329" mass="35845" checksum="827811DF131C1C0F" modified="2014-01-22" version="1">MRDDLWATPITAMVPCYDEELCIERAYREISAVMAGFAEYEILFVDDGSKDGTLDLIKSFAATDSRVRYLAFARNFGLEAAFSAGFKYASYRWTVQLDADLQSPPTEIPKLVEKALEGYDIVFGVRVDRQDPVLRRWGSSGLAFVARRLLAISLPMHASVFRVSRTSVARRIVLAGLRTPYFIATAPLIGARCAVVPTEHSARVGGTAKWSLTKLVSHAMDLFVGFSVRPLAAVQLTSAFCVLFTGVVLALLALGRIGSAGTTAAVLVVNAAAFLGLGVLTRYLIPSIRVRGELPSYLIREGNLPIDAVDDLYAHDEAPVTQHSERTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEY8</accession>
    <name>U5WEY8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562897.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41555"/>
    <dbReference type="EnsemblBacteria" id="AGZ46565">
        <property type="protein sequence ID" value="AGZ46565"/>
        <property type="gene ID" value="AFR_41555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8408"/>
    <dbReference type="eggNOG" id="COG5282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059556_0_0_11"/>
    <dbReference type="OrthoDB" id="1070352at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022454">
        <property type="entry name" value="CHP03883_F420-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018766">
        <property type="entry name" value="Zinicin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042271">
        <property type="entry name" value="Zinicin_2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39420">
        <property type="entry name" value="PTHR39420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10103">
        <property type="entry name" value="Zincin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03883">
        <property type="entry name" value="DUF2342_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03624">
        <property type="entry name" value="TIGR03624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="38307" checksum="1F5C4EB44081EDDE" modified="2014-01-22" version="1">MAQFVDWDLAAATAGALSKSGPAVSYEEATQVVAQLRDLTDEAAGHVAAYTGLTSQADPPPVRVVDRKDWAAVNIAGLKEVIIPLVTRLSGDKQPGAVADAIGSRVTGVQAGTVLAYLSGRVLGQFEVFSSEQGQLLLNAPNIVEVERKIGADPRDFRLWVCLHEVTHRTQFTAVPWMRGYFLGEVQAFVDASQNGEHILERMRRGVATLSDTLRDPDSRSSVIDIVQTPAQKAVLDRLTALMTLLEGHAEFVMDGVGPEVIPSVEAIRSKFNQRRESGNPLEKAIRRLLGIEVKMRQYAEGHKFVHGVVERVGMEGFNKIFSSPLTLPRTEELSDPDAWVARVHGPVGLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF53</accession>
    <name>U5WF53_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Spermidine/putrescine import ATP-binding protein potA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_41435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562873.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41435"/>
    <dbReference type="EnsemblBacteria" id="AGZ46541">
        <property type="protein sequence ID" value="AGZ46541"/>
        <property type="gene ID" value="AFR_41435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8384"/>
    <dbReference type="eggNOG" id="COG1125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1220708at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0129">CBS domain</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="18"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="272"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="35977" checksum="862FBEF17307045D" modified="2014-01-22" version="1">MDATAINDSGAGRTAASITLENLGKVYPDGTVAVGDISLDVGAGELAVLIGPSGCGKSTVLRMINRLIEPSKGRILIDGEDVSTQDPVELRRKIGYVIQNVGLFPHQTIRTNVGTVPKLLGWDRKKIKSRADELLELVGLDPGRYAGRYPHELSGGQRQRVGVARALAADPLVLLMDEPFSAVDPIVRGRLQEEFLRLQAAVRKTIVLVTHDIDEAVRLGDRIAVLAEGGRLMQYASPAELLSHPASQAVSEFVGTDRGIRRLAVTPVRGAMRPVGQIEEIDRLPTVEAGGTLYDALAAMLTSDSLNVVVVEDGKPVGAVSRSALFDVPNEEAAATSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF61</accession>
    <name>U5WF61_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563001.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42080"/>
    <dbReference type="EnsemblBacteria" id="AGZ46670">
        <property type="protein sequence ID" value="AGZ46670"/>
        <property type="gene ID" value="AFR_42080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8515"/>
    <dbReference type="eggNOG" id="ENOG50325FN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2314128_0_0_11"/>
    <dbReference type="OrthoDB" id="1905816at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="11331" checksum="34E3ABD7793EF6C4" modified="2014-01-22" version="1">MSTPSIAPPQRDPADVAPADSYHKADPVWVYRDGWRAGVIEASSPRAVTVTYRPGSHRGTGVDTFTASYVTSRADKDPTLDQSADKLAVRRPRLGGDGAYAMGGPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNP0</accession>
    <name>U5VNP0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357361.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38417">
        <property type="protein sequence ID" value="AGZ38417"/>
        <property type="gene ID" value="AFR_00640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.129"/>
    <dbReference type="HOGENOM" id="CLU_2614005_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="78" mass="7686" checksum="31C2632274C10EDD" modified="2014-01-22" version="1">MTFLFALGFLAVVGLAIWISRRTTGGRSSRSGTSYMHTGSSNQDGNSGGYDYGDSHHHDSGGDSGGGDSGGGGGGGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP48</accession>
    <name>U5VP48_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01415"/>
    <dbReference type="EnsemblBacteria" id="AGZ38572">
        <property type="protein sequence ID" value="AGZ38572"/>
        <property type="gene ID" value="AFR_01415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.285"/>
    <dbReference type="eggNOG" id="ENOG5031UE4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_851610_0_0_11"/>
    <dbReference type="OrthoDB" id="1128055at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="395" mass="42180" checksum="9D6F205B5981B014" modified="2014-01-22" version="1">MLKEAKADAPPPRYGVDDAVAAGRKLKARRRTLWSVGGGAALAVAVAGVVVLPQVVARQDAPATPSAAAAAAPSEAKPKAVPLTYPESRWEYAFHGYKTGKYQVNEPFLVTAGFQQATIRIGDEVEEVYGELKRPLEEGEQPKKEDYKIAYSAPGSSLLLTVYRPGAFDPALFADGTKVSVGGKSGLFKKNAHLDGDPTRKDEGHPGLAWKYAGNAWAVINTSKPNETSSKDLVAIAQGLSGAEAYPATVATKLNFVPAGYELVSGGRGADWPNGTGEFQRTNLRLVKGAEEKATGLKTPVLDDENSKVQDIRINLYRTDFSENRPPQGADQLAPYCNSGNTKLCYRMAPGGKWQVEIEGSGQEPTSELKKILAGITFASIEDGATWFPIESATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPB5</accession>
    <name>U5VPB5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative polyprenyl diphosphate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357577.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01725"/>
    <dbReference type="EnsemblBacteria" id="AGZ38634">
        <property type="protein sequence ID" value="AGZ38634"/>
        <property type="gene ID" value="AFR_01725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.348"/>
    <dbReference type="eggNOG" id="COG0142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_3_11"/>
    <dbReference type="OrthoDB" id="1861068at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="35379" checksum="E5E0EFCB3C621C0F" modified="2014-01-22" version="1">MVSIGDSALTSLGLDIDPGVDVSVSATLVAVEDELRSNVASADPLVAEAARHLMDAGGKRFRPLILALGAQMGDPTRAEIVPAALVVELTHLATLYHDDVMDEAAVRRGAPSANARWGNSVAILVGDYLFARAADIAASLGTEAVHLQARTFARLVHGQIAETVGPRGSDPIAHYLHVITEKTASLIATSARFGGLFSGVTPEQVEALTGYGETIGIAFQLSDDLLDIASESVQSGKTPGTDLREGVPTLPVLYALAGDDTDASAVRLRELLASGPLVDDALHAEALGLLRESAAMKRARETVRTYAEEARAQLAPLPAGSPRKAMESLCDFIADRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPH7</accession>
    <name>U5VPH7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02140"/>
    <dbReference type="EnsemblBacteria" id="AGZ38717">
        <property type="protein sequence ID" value="AGZ38717"/>
        <property type="gene ID" value="AFR_02140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.433"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_11_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="436" mass="46470" checksum="3896023CE17D6DE3" modified="2014-01-22" version="1">MLSTTFRSLQVRNYRLFATGQLVKLIGVWMMFTAQDWLVLDLSGNSPGALGIVTALQFLPVLLLTLWAGKLADRFDKRKLLISANAAFAVTAIVFAILVASGVVVLWHVFLFALLLGVANAIETPVRQAFVSELVELPLLPNALALSAATFNTARIGGPALAGVFLALFDTGPVFLIATVLSVAPIFSYLMMRTVDLFRSTARPSAGDTRIVDGLKYVWRRHDLLLPIVLMAAVGMIGFNFPVTLAALAKINFNAGPSTFGLLTTALAVGALGGALAGSGRRSRPSVYVVLGAALAFAVFEIAVGFSPNFIVAMILLVPTGFFSIYLAQAANHRVQMGLHSAYRGRVMALYVLVFLGTTPIGASLAGWWGEHFGVPSSIWTAGIFCLIAGVAALVWQLRSSGDHFSLRNGSRPGLTLVRTTEDEPIVEPVPARVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ56</accession>
    <name>U5VQ56_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beta-xylanase</fullName>
            <ecNumber evidence="4">3.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_03795</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-xylosidic linkages in xylans.</text>
            <dbReference type="EC" id="3.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03795"/>
    <dbReference type="EnsemblBacteria" id="AGZ39048">
        <property type="protein sequence ID" value="AGZ39048"/>
        <property type="gene ID" value="AFR_03795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.766"/>
    <dbReference type="eggNOG" id="COG3693">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020161_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="PTHR31490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00134">
        <property type="entry name" value="GLHYDRLASE10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665623" description="Beta-xylanase" evidence="5">
        <location>
            <begin position="20"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="GH10" evidence="6">
        <location>
            <begin position="62"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="40878" checksum="5703A5317443FB3D" modified="2014-01-22" version="1">MAVLATSTLLGPGLQPAQAATPAGSHLSNVPLRWAAPHDLRIGTAVAGGGHHLEQDYPDPFTYDKPYRKVLAREFNSVSPENQMKWEFIHPERGQYNFGPADAIVKFARQHGQVVRGHTLLWHSQNPAWLEEGTFTKAQLRAILKDHITTVVGRYRGKIQQWDVANEIFNENGEYRQENIFIRELGPSIVEDAFRWAHRADPKAQLFLNDYGVDWPGVKVDAYEALAKQLLADRVPLHGFASQAHLSMRYGAPDQLQAVLQRFDDLGLYTAITELDVRMDLPEGGVPTAEQLQTQATYYKTVLDACLAVADCNSFTIWGFTDKYSWVPVFFPSEGAATVMGNNFERKPAYYALRNTLAAARHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQT6</accession>
    <name>U5VQT6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiosulfate sulfurtransferase</fullName>
            <ecNumber evidence="2">2.8.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_04390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydrogen cyanide + thiosulfate = 2 H(+) + sulfite + thiocyanate</text>
            <dbReference type="Rhea" id="RHEA:16881"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:18022"/>
            <dbReference type="ChEBI" id="CHEBI:18407"/>
            <dbReference type="ChEBI" id="CHEBI:33542"/>
            <dbReference type="EC" id="2.8.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.8.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358128.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04390"/>
    <dbReference type="EnsemblBacteria" id="AGZ39167">
        <property type="protein sequence ID" value="AGZ39167"/>
        <property type="gene ID" value="AFR_04390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.887"/>
    <dbReference type="eggNOG" id="COG2897">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031618_0_0_11"/>
    <dbReference type="OrthoDB" id="920668at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="PTHR11364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="14"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="155"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="28505" checksum="4FAFFECC0192D576" modified="2014-01-22" version="1">MIQPVVDLSWVREHSDRVVLADVRWYLDGRSGRAAYEEGHLPGAVFVDLDRWLARHGSPVDGRHPLPDPGVFAQGMAAAGIGDTTTVIGYDDAGGVMAARLVWMLRATGHEAALLDGGLTAYEGELVCEVPDRPAQVFTARPWPADRLAGIDDAAGGGSVVLDARDRARFRGDTEPIDPRPGHIPGARSLPCRDNLAADGTFLPVAELRERFVSVGVTDGADVISYCGSGVTACHNLLALEHAGLGAGRLYPGSWSQYSSTDRPAATGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR82</accession>
    <name>U5VR82_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type IV pilus assembly protein PilM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359014.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05970"/>
    <dbReference type="EnsemblBacteria" id="AGZ39483">
        <property type="protein sequence ID" value="AGZ39483"/>
        <property type="gene ID" value="AFR_05970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1213"/>
    <dbReference type="eggNOG" id="COG4972">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050686_0_0_11"/>
    <dbReference type="OrthoDB" id="1750212at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005883">
        <property type="entry name" value="PilM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11104">
        <property type="entry name" value="PilM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019169">
        <property type="entry name" value="PilM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01175">
        <property type="entry name" value="pilM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="1">
        <location>
            <begin position="6"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="341" mass="36187" checksum="6398CD5EE0E2145F" modified="2014-01-22" version="1">MASVNPIGLDIGSSSIRAVEVKRAKDEYTLTNFGQVPLPPGTVQGGILEDPVAVTSALKQLWAACKFNTKQVVLGVTNPQLVVREMSVSNLPAKEMRKSLPFQVRDMLPLAVERSLLDFRPLEEPGDSPTVRGLLIAMPKDAVMSLVQAVERAGLHVVDVDLASFAMLRAASRLDAQVEAIVDIGAEVTSVVVHADGEPLIVRTVPRGGVEITASIATRLGITPPEAEALKCRYGLHGDGRPDTAAAAADAIRPLVNELRSSFTYLASGERQKQVTRVSLCGGSALMPGLAEHVQQQLGVQVMYADAAGRLRDTRRARQRGFDSFVPSAAVSIGLTLGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRG7</accession>
    <name>U5VRG7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_05615</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358943.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05615"/>
    <dbReference type="EnsemblBacteria" id="AGZ39412">
        <property type="protein sequence ID" value="AGZ39412"/>
        <property type="gene ID" value="AFR_05615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1139"/>
    <dbReference type="eggNOG" id="COG1607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050164_3_0_11"/>
    <dbReference type="OrthoDB" id="1712258at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="PTHR11049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="18530" checksum="17E31B831D6DCED4" modified="2014-01-22" version="1">MSDHLVGRATSHSRVTLSRIMNATDVNLYGTVHGGVLMKFVDDVAGASAARHSGGTAVTAAIDEILFVEPVRVGDLVHAYAQVNWTGSTSMEVGVKLTAERWDTAGGEPLTVATAYLVFVGVDVSGQPRRIPPVLPETSEDERRFREAIIRREHRLARRKAIQDARATPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRK4</accession>
    <name>U5VRK4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06395"/>
    <dbReference type="EnsemblBacteria" id="AGZ39568">
        <property type="protein sequence ID" value="AGZ39568"/>
        <property type="gene ID" value="AFR_06395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1297"/>
    <dbReference type="HOGENOM" id="CLU_670197_0_0_11"/>
    <dbReference type="OrthoDB" id="966287at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="410" mass="43088" checksum="778AF07C420A34AF" modified="2014-01-22" version="1">MVAVVALVLAAAGCDPGGSGAQDVEVVAGAGEADGGPAADAFIDGRLVDMVMDRAGTLRVLTEVDAVATLWTVQGGQLTHVAVPEVKADYVSQMTAGPDGAIYVALWNGEGGVWQIKPDGRAVHTLGIDRDTYSGDRVPPADGAPVKGAFLQYMHGVAVSADNRMYFAEERIRPTTFQLVRTVDAGKLKTVLGRDLTGLTERQWRSSRAGKGFPDGSPATQIALEGGLDNPIAVAPDGTVYAAPGRRTVVAVKPDGSARKVIGREGLDGDIDDPSEPFEARGSASEASVWLKGSGSVRQGTTNPSLVVDANGDLYLTSSRSFGEDLPESFDWTGDATGRQEELLVQSRDSLDGRTEETEVLRVKPDGSLSTVAGHADAVAVHDDWLYLARSFTDDEGDNRVLVVKTAIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRM1</accession>
    <name>U5VRM1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Purine nucleoside phosphorylase</fullName>
            <shortName evidence="1">PNP</shortName>
            <ecNumber evidence="1">2.4.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Purine nucleoside phosphorylase involved in purine salvage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19805"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer. Dimer of a homotrimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it lacks several conserved amino acids in the substrate binding pocket that confer specificity towards MTA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358998.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05890"/>
    <dbReference type="EnsemblBacteria" id="AGZ39467">
        <property type="protein sequence ID" value="AGZ39467"/>
        <property type="gene ID" value="AFR_05890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1196"/>
    <dbReference type="eggNOG" id="COG0005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_0_2_11"/>
    <dbReference type="OrthoDB" id="1364220at2"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="PTHR42679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="2">
        <location>
            <begin position="10"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="1">
        <location>
            <begin position="56"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="208"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="28710" checksum="FF6BFE0D6B05DF8E" modified="2014-01-22" version="1">MNERPPEAEIGVIGGSGLYALLDGATEHQVSTPYGEPSDPITVADVGGRRVAFLPRHGRDHRFPPHKIPYRANLWALRSLGVRQIIAPCAVGGLRPELGPGTFVVPDQLIDRTSGREQTFYDSGAVHVSFADPYCPDGRRAVLAAGETVSPVDGGTMIVVEGPRFSTRAESRWFTSIGGAIVNMTGHPEAVLARELALCYTAIALVTDLDAGVEGDHGVTQEEVFKVFADNTAKLRDVLLDAVGRLPAERTCSCRTALDGIKLPFDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSG8</accession>
    <name>U5VSG8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357598.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01830"/>
    <dbReference type="EnsemblBacteria" id="AGZ38655">
        <property type="protein sequence ID" value="AGZ38655"/>
        <property type="gene ID" value="AFR_01830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.370"/>
    <dbReference type="eggNOG" id="COG0560">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052657_1_0_11"/>
    <dbReference type="OrthoDB" id="1472357at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006385">
        <property type="entry name" value="HAD_hydro_SerB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01490">
        <property type="entry name" value="HAD-SF-IB-hyp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="28861" checksum="480C1220F55767C4" modified="2014-01-22" version="1">MGPSAAFFDLDKTVIAKSSALAFGRPFYRDGLISRRDVVKSAYAQLMFRLGGTDEQTMARTRDYLAALCKGWKVEQVQQIVAETLEELINPYVYAEAAILIGEHQAAGRDVVLVSASGDEMVRPIGALLGVTDVIATRMGIVDGRYSGEVEFYAAGPSKVDGVRELADERGYDLAECYAYSDSSSDLPLLEAVGHPSVVNPDRTLRKVALERSWPVLEFRHPVPLGRRLRDRPAVPITAAALGVGVGVAIGIALYGRHRRTRAALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSX4</accession>
    <name>U5VSX4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF402 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359584.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08820"/>
    <dbReference type="EnsemblBacteria" id="AGZ40053">
        <property type="protein sequence ID" value="AGZ40053"/>
        <property type="gene ID" value="AFR_08820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1795"/>
    <dbReference type="eggNOG" id="COG3557">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120820_0_0_11"/>
    <dbReference type="OrthoDB" id="1473648at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007295">
        <property type="entry name" value="DUF402"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035930">
        <property type="entry name" value="FomD-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04167">
        <property type="entry name" value="DUF402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159234">
        <property type="entry name" value="SSF159234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF402" evidence="1">
        <location>
            <begin position="54"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="19128" checksum="1181CD6C0EA28597" modified="2014-01-22" version="1">MGDVDVVFRKFDGSMHRRAFERFLGEDDWGTWLGVPVGTEVDYVSSGFRRKDEHRGVRLVPHGGWWTALFFAPTWDLEMYCDITAPATWKNRERVTLVDLDLDVTRRHDGHVELLDADEFAVHQLRYGYPAETVRQASAAAQEVTAACRAREEPFGLHYTTWLARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT69</accession>
    <name>U5VT69_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357794.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02810"/>
    <dbReference type="EnsemblBacteria" id="AGZ38851">
        <property type="protein sequence ID" value="AGZ38851"/>
        <property type="gene ID" value="AFR_02810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.564"/>
    <dbReference type="eggNOG" id="COG0763">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_494262_0_0_11"/>
    <dbReference type="OrthoDB" id="683757at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="527" mass="60079" checksum="B8D3703F0ADD8874" modified="2014-01-22" version="1">MWLNANSKELQWFLESAQVYWEKNLPRPADDAVILVEAFHQDIRVNLRNLAFANAIRRLHPARMVVLTGVEEQWQRTLWSGFDIGKVRDVSQAFAASEVIDVWDRVRKMTEPGAPDQLLLDYTDATYCRLAKVPSLPEGYRDRADYRLRHAYATRLFKLYQGLFEPGNVVALVTSHVDYDQWGLAVESARRAGVPIIHTQQTGCLKAYGLFPENDTGTETFRGELTRQIGDVFETQVWPRREELKPIAELVAWRAKVNLGRPSWWRGGVTASVDLNNPAERARLRSHVVDRLGFDPDRPVVTVFNHAVSDALGTNRELFPSLADWFSDTAAYAAEHPEAQWLFLDHPSQALYDSTGFFDSVAAKYKKVKHLAFRPSATLSKNALWSLTDLGVTVRGSVSNELPAYGIPVIQAGWSEWSSCGLSTVVDDQDAYWKTLEQSITAIRDGADLVTEEQVRRARLWLWLYRSGTDVVTPLVPQWEVWPADVLLKTMRATFRHIEADSDPLFTAVERMWTRKEPMLTRTDFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTD0</accession>
    <name>U5VTD0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pectate_lyase_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357938.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03530"/>
    <dbReference type="EnsemblBacteria" id="AGZ38995">
        <property type="protein sequence ID" value="AGZ38995"/>
        <property type="gene ID" value="AFR_03530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.710"/>
    <dbReference type="eggNOG" id="COG5434">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009451_0_0_11"/>
    <dbReference type="OrthoDB" id="32528at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14490">
        <property type="entry name" value="CBM6-CBM35-CBM36_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033801">
        <property type="entry name" value="CBM6-CBM35-CBM36-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024535">
        <property type="entry name" value="Pectate_lyase_SF_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12708">
        <property type="entry name" value="Pectate_lyase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666055" description="Pectate_lyase_3 domain-containing protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate_lyase_3" evidence="2">
        <location>
            <begin position="245"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="668" mass="70448" checksum="C5DCB03D0342B68E" modified="2014-01-22" version="1">MTISARWPALATASVVGLGLAVGAGVPASAAPQAPVVTRAGLDPALVAGRGATVDFAEQEAENTKHNGAVIGPDRAAYSLAAEASGRTAVKLTPGQYVEFTLPKAANAVNVRYSIPDAPTGGGITAPLDVAVNGKHTKKMTLTSEYAYLYNQYPFSNDPTAGLLHPDWWIAECACVPAATTPTPEISKPFRPMHFYDEQRLLLGKTYRAGDRIRLTASSGAAWTVIDLLDSQLVGLPHVNLVASNVLLFGADPTGKRDSAAAFDKAIAFAKKAKLKVYVPPGTYQVNRHIIVDNVTIEGAGSWYTIIKGKEVALSEPAPDGSVHTGVGFYGKDAADGGSRNVHLKGFTIQGDVRERIDTDQVNGIGGALSDSTIDGLYLRHTKVGMWFDGPMTNLKITNNTIVDQIADALNFHTGVTNSVVRNNFIRNTGDDGLAMWAEKITNTGNTFDHNTVQTPTLANGIAIYGGTDNTVSNNLVADPIREGSGIHAGSRFGAEPFAGALTIKNNTVVRAGTLELNWNIGLGAIWFYALDKTIDADIQVTGDHYLDSTYNAIMLVSDWGVKDLYSIEGVKFKDLRIDGTGTSVVSARVAGSASFSNVDARNVGAVGVNNCGSFGFKPTGSEFSLTDLGGNDGGGTTGPWLAAWELPNTITCDDRPPVVAPPAPTTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTN7</accession>
    <name>U5VTN7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358000.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03830"/>
    <dbReference type="EnsemblBacteria" id="AGZ39055">
        <property type="protein sequence ID" value="AGZ39055"/>
        <property type="gene ID" value="AFR_03830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.773"/>
    <dbReference type="eggNOG" id="COG2771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2630149_0_0_11"/>
    <dbReference type="OrthoDB" id="1036370at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="78" mass="8670" checksum="7037EDF5BD5687DA" modified="2014-01-22" version="1">MPKITLTDRERELVALLAQGHTDVSAAEQLGISARSVTYVLRSLMDRLGVENRFQLGLALGFLRKAHVPPVEDRGDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTU1</accession>
    <name>U5VTU1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-glutamyl phosphate reductase</fullName>
            <shortName evidence="7">GPR</shortName>
            <ecNumber evidence="7">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            <shortName evidence="7">GSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">proA</name>
        <name evidence="9" type="ORF">AFR_04155</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04155"/>
    <dbReference type="EnsemblBacteria" id="AGZ39120">
        <property type="protein sequence ID" value="AGZ39120"/>
        <property type="gene ID" value="AFR_04155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.839"/>
    <dbReference type="eggNOG" id="COG0014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030231_0_0_11"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0521">NADP</keyword>
    <keyword evidence="6 7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="8">
        <location>
            <begin position="7"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="412" mass="42891" checksum="FD7BBB99FFFD0DF5" modified="2014-01-22" version="1">MTVLEQAAEARVAAIDLATATRADKDAALLLMADRLVERAGDIVTANAVDVENARAGGLSEAMIDRLTLTTERVAAMAEGLRQLAALPDPVGDVVRGSTLANGLELRQVRVPFGVVGIIYEGRPNVTADAAGICLKSGNAVLLRGSGSAFSSNAAIVSVLRKAVADSNLPANAIQLLDATTRDSVKELMRARGLVDVLIPRGGASLIKAVVEESTVPVIETGVGNCHVYVDEGADLEKALGVVLNSKTQRLSTCNTAESLLVHATLASSFLPLVVEELIAAGVTVHGDPRVAGLDGVVPATDEDWGTEYLSADISVAVVDSLDDALDHIRRYGTGHTEAIVTENLAAARRFSARVDAAAVMINASTRFTDGGEFGFGAEIGISTQKLHARGPMGLPELTSTKYIVTGNGHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUQ1</accession>
    <name>U5VUQ1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inositol-phosphate phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_11375</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360549.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11375"/>
    <dbReference type="EnsemblBacteria" id="AGZ40564">
        <property type="protein sequence ID" value="AGZ40564"/>
        <property type="gene ID" value="AFR_11375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2318"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_0_2_11"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052832">
        <property type="term" value="F:inositol monophosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052833">
        <property type="term" value="F:inositol monophosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="26964" checksum="4F46480127D17A54" modified="2014-01-22" version="1">MTDYADLLPVAHQAVDLASSIMLSMQPGVLTAKGDRDMASEVDFAVEKQVRAFLAERTPDIAFLGEENGSSGNTDGLMWALDPVDGTVNFVHGSPLCAISLGLISEKQSVLGVIDLPFLGSRYSAAAGHGAESDGQRIRASGVGDVSEAVVAIGDYAVGAGAEEKNELRFALTGRLAARVQRIRMHGSAAVDLAWLAAGKIDAVVMLANKPWDTAAGVVIAREAGALTVDRDGTSHSFDAQATIAANPGLLPLILDLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUV3</accession>
    <name>U5VUV3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Luciferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360546.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11365"/>
    <dbReference type="EnsemblBacteria" id="AGZ40562">
        <property type="protein sequence ID" value="AGZ40562"/>
        <property type="gene ID" value="AFR_11365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2316"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_7_0_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019921">
        <property type="entry name" value="Lucif-like_OxRdtase_Rv2161c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03619">
        <property type="entry name" value="F420_Rv2161c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="30756" checksum="27455A60F4EE026D" modified="2014-01-22" version="1">MDFGVGYFPTHDGIGPGALARHVEERGQSALYFAEHTHIPASRDSPWGDGSRELPRKYWHTYDLFVALTAAAAATTTLRVGSGVCLVVERDPIITAKEVASIDHLSGGRLDFGVGAGWNREEMANHGTDPRTRIALLSDRVQAMQAIWSQDEASYAGRFVNFDRIWSWPKPVQNPWPPVLIGGGGPTVLDRVLAWADGWFPQWHDRDLFERIAELEARADRPIQVQVLSVPPDPKVLEQLANAGVHRASSWLPSGPWDQVEPGLVRWERAIADFNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUW1</accession>
    <name>U5VUW1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Deferrochelatase</fullName>
            <ecNumber evidence="6">1.11.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peroxidase EfeB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_05760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the recovery of exogenous heme iron. Extracts iron from heme while preserving the protoporphyrin ring intact.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2 H(+) + heme b = Fe(2+) + protoporphyrin IX</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.99.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:22585"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="6">Binds 1 heme b (iron(II)-protoporphyrin IX) group non-covalently per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the DyP-type peroxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358972.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05760"/>
    <dbReference type="EnsemblBacteria" id="AGZ39441">
        <property type="protein sequence ID" value="AGZ39441"/>
        <property type="gene ID" value="AFR_05760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1169"/>
    <dbReference type="eggNOG" id="COG2837">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039488_2_0_11"/>
    <dbReference type="OrthoDB" id="837319at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033212">
        <property type="term" value="P:iron import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006314">
        <property type="entry name" value="Dyp_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006313">
        <property type="entry name" value="EfeB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04261">
        <property type="entry name" value="Dyp_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01413">
        <property type="entry name" value="Dyp_perox_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01412">
        <property type="entry name" value="tat_substr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51404">
        <property type="entry name" value="DYP_PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 6 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014488212" description="Deferrochelatase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="424" mass="44960" checksum="8AE5B2F602B0C111" modified="2014-01-22" version="1">MSDSSLSRRRAIALAGVGVAGVTGAAAIGSALVKGSAGDTTPHPVEGAVAFTGEHQAGIVTPAQDRLHFVAFDVITKDRERLIGLLKDWTAAAARMTAGRDAGEIGAVGGVPEAPPDDTGEALGLPPSGLTITVGFGPTLFRDATGKDRFGLAAKRPPALDDLPHFPGDVLDDSLSGGDLCVQACANDPQVAVHAIRNLARIGMGVVSVRWSQLGFGRTSSTSTTQTTARNLFGFKDGTANLKLEETDLLREHLWVQPGDGPDWMTGGSYLVSRKIRMIVETWDRSSLAEQEMIVGRQKGTGAPLGKSGEFEEPDLEAKGADGEPLLALRSHVRLAHPTTNGGARLLRRGYNFVDGSDGLGRLDAGLFFIAFQRDPRKQFVPIQRSLSRQDEMNEYLKPVSLGLFACPPGVSGPDDFWGRALFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV14</accession>
    <name>U5VV14_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12090"/>
    <dbReference type="EnsemblBacteria" id="AGZ40704">
        <property type="protein sequence ID" value="AGZ40704"/>
        <property type="gene ID" value="AFR_12090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2461"/>
    <dbReference type="eggNOG" id="ENOG502ZEH0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830813_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="16127" checksum="50C35C0F13CA941C" modified="2014-01-22" version="1">MRPFHADRADVVRAARRLLGTRLRPMLFKQIPTRSLGTGAGPVWTWMPYRPPARRRAAVFVTDLGLATPLPDELPAAEYEWAELFADLRSRGVVPVVLSPYRPERASARLRRRAVIVQWDRGLTERAVRQPVRARLRRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV84</accession>
    <name>U5VV84_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359141.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06605"/>
    <dbReference type="EnsemblBacteria" id="AGZ39610">
        <property type="protein sequence ID" value="AGZ39610"/>
        <property type="gene ID" value="AFR_06605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1348"/>
    <dbReference type="eggNOG" id="ENOG5030VH7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2285364_0_0_11"/>
    <dbReference type="OrthoDB" id="2212935at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="101" mass="10846" checksum="C080CE5561061B30" modified="2014-01-22" version="1">MNGDIAEDPLARSVLNHLAKTRGKDDPLGSLARTVISGEATLRTAADNSWHSQGLAEAAQAAQDEQNRMTPEQRADYESAARRLMNDTAADDTDDAPGRNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVB1</accession>
    <name>U5VVB1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40747">
        <property type="protein sequence ID" value="AGZ40747"/>
        <property type="gene ID" value="AFR_12305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2498"/>
    <dbReference type="eggNOG" id="COG4292">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045667_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010640">
        <property type="entry name" value="Low_temperature_requirement_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840">
        <property type="entry name" value="PTHR36840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06772">
        <property type="entry name" value="LtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="390" mass="42130" checksum="13EA59C5A69A9CB8" modified="2014-01-22" version="1">MPMWPRDPNETGRTASSLELFFDLVFVVAVSIASVQLHHQLSEAHLFEGIVNYAVVFFGIWWAWMNFTWFATSFDTDDWLYRLLTIVQMGGVLVLASGIQAAFEDQDYVVLVLAYVVMRIALVALWVRASRSAGDARRSTTTYAVGIAAVQILWLASLLLPAAAFWAAVIVLFGAELSVPIFAERRGGTPWHPHHLSERFGLFTLILLGESLLASANAIIEALHEGDAVGRLIGVAVLTLVVTAALWWVYFWAPHHTAIGGLGLSLGYGYGHYFIFAAAGALSAGIEAEVDVITGHSELHPVVASFAYTVPIAVFILGTWLLVMRRSAGRHINVIVPAGAVLVLVDPLIPVPFALTAVILTGVVVALVWHDPLDRTTQLPAGSGDTAVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVP5</accession>
    <name>U5VVP5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359242.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39711">
        <property type="protein sequence ID" value="AGZ39711"/>
        <property type="gene ID" value="AFR_07110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1451"/>
    <dbReference type="HOGENOM" id="CLU_3021446_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="61" mass="6618" checksum="2BBEA13A91D1A2C2" modified="2014-01-22" version="1">MTQQNERLPAFGDEEAVPDGDSDFASEHSDTVVDPEITEGKDDGETESPRGWTGLDREGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVT3</accession>
    <name>U5VVT3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the fructosamine kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361127.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13910"/>
    <dbReference type="EnsemblBacteria" id="AGZ41068">
        <property type="protein sequence ID" value="AGZ41068"/>
        <property type="gene ID" value="AFR_13910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2822"/>
    <dbReference type="eggNOG" id="COG3001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036517_0_1_11"/>
    <dbReference type="OrthoDB" id="1457558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016477">
        <property type="entry name" value="Fructo-/Ketosamine-3-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12149">
        <property type="entry name" value="PTHR12149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03881">
        <property type="entry name" value="Fructosamin_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006221">
        <property type="entry name" value="Ketosamine-3-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="294" mass="32573" checksum="B5F194E2EA284B78" modified="2014-01-22" version="1">MTDSATFLRGCLDRAGIGDVVALEPVAGGLAALAGMATRRDAPPVFVKAFADAPDDDVFAAEADGLTALRKLGGVETPDVIQADRELLVLSVLQPRPGGEDFWEQLAHVLARLHLTTVNPRFGWHRDNWLGRRRQINTWADDGFAFFAEHRLLRWLGEPRVEAALDAGDRAALERLCRRLPDLLPDRPACLTHGDLWTQNLLATADGRPALIDPAVSYLWAEVDLAHLWTTSPPPEAHRFFALYAELTSLDADWQARMPILQLRQHLAVLAQFDNDWGAADLIRATLAPFRTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVT4</accession>
    <name>U5VVT4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative transcriptional regulatory protein (TetR-family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_13490</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361043.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13490"/>
    <dbReference type="EnsemblBacteria" id="AGZ40984">
        <property type="protein sequence ID" value="AGZ40984"/>
        <property type="gene ID" value="AFR_13490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2739"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_40_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="18"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="21392" checksum="2E7117515DC82CB4" modified="2014-01-22" version="1">MTTRPALDVTARQRREGDQLRGELLEAAAVLAATPRPVAVPSLRAVARACGVSATAVYRHFPSQAALTHAVITVHHQAFVAAVLRADDPAGDPEVRLHRLAVGYVRWGLANPGMYQLLFESADQLDAESGLGDITDEVMKLASTLLAELTGTAPAEVAVLAERLWFGLHGIVSLRMHKPDQTWHVAADEAAVTVAQQILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW62</accession>
    <name>U5VW62_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_13915</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361128.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13915"/>
    <dbReference type="EnsemblBacteria" id="AGZ41069">
        <property type="protein sequence ID" value="AGZ41069"/>
        <property type="gene ID" value="AFR_13915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2823"/>
    <dbReference type="eggNOG" id="ENOG50331V5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_775052_0_0_11"/>
    <dbReference type="OrthoDB" id="2135880at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="SSF49464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666135" evidence="3">
        <location>
            <begin position="32"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="345" mass="34614" checksum="77A3C4EEB23311F1" modified="2014-01-22" version="1">MTSTGARWSRPAASIVLAGAVLAVSAAPVAAAPPAIRIQSVSSDQVKSGETVRVRFKATNNNKRTEKVFVAVSGGLRCTAGCSAAPDIRAGQSRTFEATVVAPKVSAGEESGRNLAVSVRIGRQTAFDHKMILVRGANTSPSAVSRISGRVRDAGGDAIGGATVAVRDSAGHDYRTTTNAGGKFTITSNDSRPIAAGPITVAAAKDGYRSARATVQGAGGRAATVRLTLAAVASPSKAPSSPAAVASVPPVAEEEPATEESVTAAPATTEKVDGEGSGLLLFTILGGLLIAAGVGTLALMMIRRRAPASDPTMAAPTGAGGSGMADAPTAVLRTVPHDYGRPTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWS5</accession>
    <name>U5VWS5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09355"/>
    <dbReference type="EnsemblBacteria" id="AGZ40160">
        <property type="protein sequence ID" value="AGZ40160"/>
        <property type="gene ID" value="AFR_09355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1907"/>
    <dbReference type="HOGENOM" id="CLU_202636_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="49" mass="5819" checksum="B3365C82D07D57C8" modified="2014-01-22" version="1">MATLMQRLQMFLRSPQGQRIVQKGQQQLAKPENQARLRKIATRIQSRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX35</accession>
    <name>U5VX35_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">APH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361493.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15760"/>
    <dbReference type="EnsemblBacteria" id="AGZ41434">
        <property type="protein sequence ID" value="AGZ41434"/>
        <property type="gene ID" value="AFR_15760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3200"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079051_0_0_11"/>
    <dbReference type="OrthoDB" id="1457558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="51"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="313" mass="34123" checksum="65FE60B852B67E96" modified="2014-01-22" version="1">MTGDWRDGGFRQRRPSAETLAWVAASMGPGSRVVGFRRMTGGVCSAVNRLTVERGGKRTSVVLRQYPDGLGLRDTLEQEIANLGVVAGSGLPVPSVLASDVAGASTGGTPSLLMTRLPGHVHLDPAEPGSWLTRIAELAVVLHSLDLPAKTFRPWTDSWIAPRDGFQVPVDAPRPAVWKAAFRAMAEPPPKDTAVFLHCDFLSVNLLWSRGKITGLTDWNSLHRGSRAIDVGHCRRYLAALFSPEWSEQLRSLYESAAGVTLDPWWDLYALLHYDNSGSKWIRGQVAGRRPVDVPGMTSRVEVAVESALRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX85</accession>
    <name>U5VX85_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">dTDP-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="4 6">4.2.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_16035</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O</text>
            <dbReference type="Rhea" id="RHEA:17221"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="EC" id="4.2.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="4 6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361558.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16035"/>
    <dbReference type="EnsemblBacteria" id="AGZ41489">
        <property type="protein sequence ID" value="AGZ41489"/>
        <property type="gene ID" value="AFR_16035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3254"/>
    <dbReference type="eggNOG" id="COG1088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_14_11"/>
    <dbReference type="OrthoDB" id="707977at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01181">
        <property type="entry name" value="dTDP_gluc_dehyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="7">
        <location>
            <begin position="4"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="324" mass="34974" checksum="9C3797AEDCC42B62" modified="2014-01-22" version="1">MQIFVTGGAGFIGSAYVRALLQDEYPGAAGASVTVLDLLSYSGNRANLPVADSRLRFVQGDIADAALLRDLLPGHDAVVHFAAESHVDRSIAGAVPFVTTNVLGTQVLLDAARTAGIGRFVHVSTDEVYGSIAEGSWTETWPLMPNSPYAASKAGSDLLALAAHRTHGMDVVVTRCSNNYGPYQFPEKVIPLFVTNLLDGKQVPLYGDGGNIRDWLHVADHCRGIQLALEKGRAGEVYHIGGGTELSNRELTGRLLDACGAGWDMVRPVEDRKGHDRRYSLDITKISEELGYAPQVTLDEGLASTVAWYRDNRDWWEPLKAGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXH1</accession>
    <name>U5VXH1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361811.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16950"/>
    <dbReference type="EnsemblBacteria" id="AGZ41668">
        <property type="protein sequence ID" value="AGZ41668"/>
        <property type="gene ID" value="AFR_16950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3440"/>
    <dbReference type="HOGENOM" id="CLU_1567344_0_0_11"/>
    <dbReference type="OrthoDB" id="1802502at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665824" evidence="1">
        <location>
            <begin position="28"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="17256" checksum="7750D837D341C7F8" modified="2014-01-22" version="1">MRTTLLMAGVVGLLLSTAACGSTSTEAGELTTATSLLPASSTEYTFVVPDEGADFVDPATGEPPTPCRTGELRITGGDQALIFENITGHACSLHGHPAIGSLTPAGDKTQAILLSPGDRVQAVLRPATKACDPVRVKGLKVSAPEDPATVVVDAPDTCASAVEAFTPIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXS9</accession>
    <name>U5VXS9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360386.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10935"/>
    <dbReference type="EnsemblBacteria" id="AGZ40476">
        <property type="protein sequence ID" value="AGZ40476"/>
        <property type="gene ID" value="AFR_10935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2227"/>
    <dbReference type="eggNOG" id="ENOG50332QW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1894691_0_0_11"/>
    <dbReference type="OrthoDB" id="1667181at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="13866" checksum="3160C694DB45F378" modified="2014-01-22" version="1">MKAPTDLEHLTDFFDRYGSALTRGDIAAVAGCYALPGMVVADTYSFTFTSPAAVALSFLGAAPAYKEQQIVAAHAQLRDVQRLSTALAMVAVEWEYLDSFGNAVPGECYRYLIRSGADGPRITTVIATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXT9</accession>
    <name>U5VXT9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH-type transcriptional regulator betI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361652.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16475"/>
    <dbReference type="EnsemblBacteria" id="AGZ41577">
        <property type="protein sequence ID" value="AGZ41577"/>
        <property type="gene ID" value="AFR_16475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3341"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_10_1_11"/>
    <dbReference type="OrthoDB" id="2089619at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="199" mass="21355" checksum="1FE7531C04C5DF27" modified="2014-01-22" version="1">MTTSTSGPRRRDAAGTRRLLLDAAQHRFARNGYTATTVRDIADDAGVNVALISRYFTSKEGLFEACLLGAVDELGRTVTEDVTLDQVPHTIAAQLAGPHTGQFPNRLLLLLRSSGDERADRIRLDILQSFSERLAAAAGLRAGDPDREELVLRAQVTLAASFGIALLRASTALEPLSSAGRDDLLGPLQDLVGGLLRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXW9</accession>
    <name>U5VXW9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RibD_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11135"/>
    <dbReference type="EnsemblBacteria" id="AGZ40516">
        <property type="protein sequence ID" value="AGZ40516"/>
        <property type="gene ID" value="AFR_11135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2268"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="7"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="20787" checksum="4560C7418AC936FB" modified="2014-01-22" version="1">MLGGMTKVVADISVSLDGFVTGPDPDVEHGLGTGGEALHTWAFSSDPVDREVLATATDATGAVVMGRRLFDIIDGPHGWNDEVGYGAGLAAPPPVLVVTRNPPEKVRLADRVTFVVDGVSSAIAKATAMADERDVVVMGGAEVVRGALEAGLVDELRLHLAPVLLGGGTPLFTGGAAPRELRQLHVRVSAHATHLTYRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY21</accession>
    <name>U5VY21_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362271.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18100"/>
    <dbReference type="EnsemblBacteria" id="AGZ41898">
        <property type="protein sequence ID" value="AGZ41898"/>
        <property type="gene ID" value="AFR_18100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3673"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_1_11"/>
    <dbReference type="OrthoDB" id="1367386at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="15"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="29919" checksum="2B12BF3A71EE8674" modified="2014-01-22" version="1">MTSPSVPADPIPVGRLGFGAMHLPTAPEPDRRAAIAVARRAVELGVTLIDTAHLYGWGANEELLAAALYPYPENLILATKVGVAPPRGDREAAFDGSPAFLREQVDEALRRLRVDRLDLLQLHRIDPAVPLADQLGTLRDLQTAGKIAHLGLSEITAAQLAEARTIITVASVQNRYNVLDREHETVLDACTEAGIAFLPWRPVHPATTTTGTEIAAVAAELGATQAQVSLAWLLARSPIMLPIPGTASITHLEENLAAEGLRLSPDQYDRLTAAAFPRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY42</accession>
    <name>U5VY42_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integral membrane sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_17655</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362091.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17655"/>
    <dbReference type="EnsemblBacteria" id="AGZ41809">
        <property type="protein sequence ID" value="AGZ41809"/>
        <property type="gene ID" value="AFR_17655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3579"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_8_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665474" evidence="2">
        <location>
            <begin position="23"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="3">
        <location>
            <begin position="184"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="39048" checksum="9D0E6DEBEE4316E6" modified="2014-01-22" version="1">MHRRLRRVRVVTLVVLATNAAAAWFMPAIGLGREPDQARVVWGLTGIVLFAVAQAVVLRALVTPWISERARRRSIVVFAGASLVSVPLVGPLGDSWPSWAWLAACIVGIVPVLTGKVAAALLCTLVAVISVVISPGSVVDTVLITVGFGSGIAAINGLHVWFWDLLLQAEEGRSAQAQLAATEERLRFARDVHDLLGHRLSVIALKAELAERLAPSDAGRAGAEAAEVRRIAATALTELREVVHGYREVDLREQVSAIERVLVSSGVRCTVTTPAGELPGPGAAVLAAVLREASTNLLRHSAATWCTIDVEDSAGGVRLTVVNDGAGAATPDAHSHGLRGLAERLAEHGGQLRTRAADGTFTVEAVVRSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYE2</accession>
    <name>U5VYE2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PMT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_18155</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18155"/>
    <dbReference type="EnsemblBacteria" id="AGZ41909">
        <property type="protein sequence ID" value="AGZ41909"/>
        <property type="gene ID" value="AFR_18155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3685"/>
    <dbReference type="eggNOG" id="COG1807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027561_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="Glyco_trans_39/83"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="PMT" evidence="8">
        <location>
            <begin position="121"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ41909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="522" mass="56536" checksum="3D86C0B5C9347EB9" modified="2014-01-22" version="1">MATEAPTQVIILAPVPDQIEAPPPPPRGSRTDLYVALGLTAVVLFFLAWNITGFPTATDDEGTYLAQAWAVQNGHGLAHYTYWYDHPPLAWIQLAGLAWVPAVLAPDLLAVAGGRIAMIPVVAASLLLVYLIGRRLEFSRWAAASALLIFGLSPLAITMNRQIYLDSFAVAWMLGALALALSPRKHLWHYAAAGAATAVSVLSKETMLITVPAVLVAMWQNAARTSTRPWAFGGYTSGIVLVGVFYPLYALLRGELFPGEGHVSLIGAWQFQLMSRSGTGTVLDEGSGTHSLITSWLYYDAVILAAGLVATVVALAVRRLRAPAIAATILAVVALRPGGYLPAMYVVQVLPFFALVIAGVADEGVRRLRPERSWWRWSIVGLAAVTALALVVPRWYVGVERALTTDDNASYLAAANYLRNDLPDQPDTTIVLDDVLWLDAVNAGYSRENVIWFYKLDLDTEVAARLPGGWRDVDYIVSTPALRQDLSTLPTVTKVLANSTPVASFGPVDGRIEIRRVDKEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZL5</accession>
    <name>U5VZL5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361140.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13975"/>
    <dbReference type="EnsemblBacteria" id="AGZ41081">
        <property type="protein sequence ID" value="AGZ41081"/>
        <property type="gene ID" value="AFR_13975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2835"/>
    <dbReference type="eggNOG" id="COG2340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048111_3_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="16"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="133" mass="14625" checksum="E266A3485544C8E2" modified="2014-01-22" version="1">MMRTPVREDLERRVVAAVNEERRRARLGRLVAEPLLAVAARAHSDDMAQRGYFSHHSPEGTTAGDRVAVTGYQFAVVGENIAWGQRTAREVMTAWMNSRGHKANILDAGFTRIGVGVAADRHGSPVWTQVFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZU9</accession>
    <name>U5VZU9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42297">
        <property type="protein sequence ID" value="AGZ42297"/>
        <property type="gene ID" value="AFR_20125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4082"/>
    <dbReference type="eggNOG" id="ENOG5033V8H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071553_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="24828" checksum="26CFBA8A2633FB73" modified="2014-01-22" version="1">MPYARPDGTAEKLTHLPRLASWEKSTHPGQIRLLDYLSSTRLLLEPVLARLTGPLALRLDIGLPETIKLLDHHDLDNYLYPLAAHLRKQAGRDFVSVWATKYTGPDSFVRVEQARPVVFPVRSTIRTTVSSGKVGYKEQIRDQLTGVTVLPDGPVRLEVAFTVGPARSWTTLWKPTIDALGAVLGIPEGGRPWSPRDGRIDRLGLHCSVAATLGNDVLIDLAWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W016</accession>
    <name>U5W016_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ROK family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362744.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20500"/>
    <dbReference type="EnsemblBacteria" id="AGZ42372">
        <property type="protein sequence ID" value="AGZ42372"/>
        <property type="gene ID" value="AFR_20500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4157"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_0_11"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH crp-type" evidence="2">
        <location>
            <begin position="21"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="38155" checksum="DB670F42FAD5BAF9" modified="2014-01-22" version="1">MHRSAADQSFLRGYNEALVMAVARSMPTFERATVADATGLTPQAVSKVLGRLVDDGLIAPAGVRRAAIGKPAGVYELVPASRFAIGAHVARRTLRLVLTDLAGTVHHSAVTPLPDDFTPAQLLDELAIGVKTITAAFRPGDRLTGVGLGMIGPLDHAQGTVRDAHRLRHWHDVPLRALAEEALDLPVHLDKDVTAGVTAEAWRHGPQFRDAALIMVESGVGAGLWLGGTAHRGAHTNAGEFGHTVVALDGPPCVCGRRGCLEAVHDRAAEAGDITRAARVLAIGVVNLLQTLDVAHVVLAGADLLQHATTYHEAVRQAVSTEVPRADWQSADVTLSSLGADVIAAGAAMQVLDLRYGIPGLAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W025</accession>
    <name>U5W025_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362861.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21085"/>
    <dbReference type="EnsemblBacteria" id="AGZ42489">
        <property type="protein sequence ID" value="AGZ42489"/>
        <property type="gene ID" value="AFR_21085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4274"/>
    <dbReference type="eggNOG" id="COG1716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108862_1_0_11"/>
    <dbReference type="OrthoDB" id="1632705at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SSF49879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="68"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="150" mass="16157" checksum="1A4380AEB1EBB09E" modified="2014-01-22" version="1">MTRPDDEFPPLDVTSTLNLGALDEVLEGPETDVVPSRMSGSLPPGMALLVVRRGPNAGARFLLDHDVTTSGRHPDSDIFLDDVTVSRRHAEFHRDGGTFTVRDVGSLNGTYVNRERVESATLSNGDEVQIGKFRLVFIAGPRPEGEGSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0D1</accession>
    <name>U5W0D1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH merR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21100"/>
    <dbReference type="EnsemblBacteria" id="AGZ42492">
        <property type="protein sequence ID" value="AGZ42492"/>
        <property type="gene ID" value="AFR_21100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4277"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092079_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="17367" checksum="426607E876B75E2E" modified="2014-01-22" version="1">MTACQAVGISYRQLDYWARTTLVVPSIRDASGSGTQRLYSFRDLVVLKVVKRLLDAGVSLQNIRRAIETLRSRGVGDLAGITLISDGTTVYECRSPEEVVDLLQGGQGVFGIAIGGAFKEIQGSLSHLPAEPVSSPQPVGTNLPAESTAGDELAARRARRRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0H3</accession>
    <name>U5W0H3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_16015</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + 2 NAD(+) + UDP-alpha-D-glucose = 3 H(+) + 2 NADH + UDP-alpha-D-glucuronate</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361550.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16015"/>
    <dbReference type="EnsemblBacteria" id="AGZ41485">
        <property type="protein sequence ID" value="AGZ41485"/>
        <property type="gene ID" value="AFR_16015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3250"/>
    <dbReference type="eggNOG" id="COG1004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_2_11"/>
    <dbReference type="OrthoDB" id="647136at2"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="11">
        <location>
            <begin position="320"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="153"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="253"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ41485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="437" mass="46576" checksum="E072FFA0D5980DC2" modified="2014-01-22" version="1">MNLTVIGTGYLGATHAICMAVMGHDVVGVDVDASKVERLSSGEVPFFEPGLPELLTKALESGRLRFTTSFQEAGEFGDVHFICVGTPQRKGSEAADMTYVDASVTELSKHLHRRALVVGKSTVPVGTAARLAELIRTTAPAGDQVELAWNPEFLREGFAVDDTMKPDRMVFGVTSAWSEEQLRAAFAPVLAQGVPVKVTSLQTAELVKVAANSFLATKISYINAMAEVCEATGADVHDLAEALAYDERIGGKFLRPGLGFGGGCLPKDIRAFAHRAEELGVGQAVGFLREIDGINGRRRARTVDLVVELCGGNVAGKKIAALGAAFKPNSDDIRDAPALDVASTLAGLGADVHVFDPAAMDNARKAHPELSYGSSAFDVARDADVVVLLTEWTEFREIAPEAMAEVVGARRIFDGRHALNSDAWRAAGWEYKALGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0T8</accession>
    <name>U5W0T8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_16365</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361630.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16365"/>
    <dbReference type="EnsemblBacteria" id="AGZ41555">
        <property type="protein sequence ID" value="AGZ41555"/>
        <property type="gene ID" value="AFR_16365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3319"/>
    <dbReference type="eggNOG" id="COG1506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028869_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665945" description="CBM2 domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="3">
        <location>
            <begin position="437"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="537" mass="54287" checksum="295D4F111FB611D8" modified="2014-01-22" version="1">MTTSSGRPRIHPLIVMLVAGTLTVAGATTLLPVTAPAEAAVLAAGVEDEGAGCAVPALPEAGALPSITKLPDPFLRLNGTRITSATDWQCRRAEIKKLAEKFVYGEKPAKPASVSGTVSSSSISVNVAHNGKSSSFSARVELPSGTGPFPAVVVLGGFGADTAAIKAAGAAVINYDPYAVGKEGTPRNNKQGAFYSIYGASSSTGLLMAWAWGVSRIIDVIEQSNGSILKADATGVTGCSRFGKGAFVTGAFDQRIALTMPIESGSAGAPIFRGIPGEGAQSLSSAYGEQPWLGDAFGTFTGSPARLPVDTHETVAMVAPRGLFVMDNPHIANLGPKSASVAALGGAEVYRALGAGSNITYWSDVTDGTHCASRAEWRTPLQQHIQKYLLGTGNAAGTMRISSRAAGNLAEWRDWPTPVLGSTPPPASSGPPPSSGPPPASGGCVATVSVNAWTGGFVATVQVTAGAAGTSAWTVGLTLPSGATITGIWNANRTGTSGAVQFTNVTHNGRLGGGQATEFGFQATGSSGGLTPTCSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W102</accession>
    <name>U5W102_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_16420</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361641.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16420"/>
    <dbReference type="EnsemblBacteria" id="AGZ41566">
        <property type="protein sequence ID" value="AGZ41566"/>
        <property type="gene ID" value="AFR_16420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3330"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="101"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="18769" checksum="78D772F426BB0197" modified="2014-01-22" version="1">MKKSDADYVAYVQGRLPALRRLAYHLAGDSHHADDLVQETIAKLYAVWGRISGSDGLDAYTRTMLVRAFLDEKRRGWWRVRLFDAVPESAGQPPPGVEERLLLVAALKRVPPKQQATLVLRFLHDLPIAEVATILGCSEGTVKSQTTHGLATLRRLLTKWSSPSATAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W164</accession>
    <name>U5W164_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22410"/>
    <dbReference type="EnsemblBacteria" id="AGZ42752">
        <property type="protein sequence ID" value="AGZ42752"/>
        <property type="gene ID" value="AFR_22410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4543"/>
    <dbReference type="eggNOG" id="ENOG5032SCT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078512_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="18061" checksum="6EF30F50EAE741FC" modified="2014-01-22" version="1">MPTRFEPDRWLALRRSPPFYPDAVSLTERPRDILAGIDLSPGCSVKDSFATLDLAPAGFEVLFDAEWIYREPVESPGPSRYPRTDRPVVEVEGAIGNLSDGCVGISNVRGDDVWEDAVAAISAHFPGLPLVGYETADTLPAAHAAGFRSIGPLRIWMIPESADPAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1I7</accession>
    <name>U5W1I7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23085"/>
    <dbReference type="EnsemblBacteria" id="AGZ42887">
        <property type="protein sequence ID" value="AGZ42887"/>
        <property type="gene ID" value="AFR_23085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4678"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_1_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="8"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="172" mass="18338" checksum="11BC0C47128EC54C" modified="2014-01-22" version="1">MRAVLASSDEPTVIVGHSYGGIVAAEAAVGVEPVRHLVFVSSYLPEPGESLSTFGAPEPPQFLDFDPEGGTFGAVPELFAETFVQDCPPEIIQEGTARLTRQTITVTQQPVQAVAWHDLPTTYVVCTEDRGTPAAAQREFARRADKVVEVAAGHHPFLSQPETIADLLAGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1U6</accession>
    <name>U5W1U6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cholesterol esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363659.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24005"/>
    <dbReference type="EnsemblBacteria" id="AGZ43069">
        <property type="protein sequence ID" value="AGZ43069"/>
        <property type="gene ID" value="AFR_24005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4863"/>
    <dbReference type="eggNOG" id="ENOG50312Q2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092426_0_0_11"/>
    <dbReference type="OrthoDB" id="1665716at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046198">
        <property type="entry name" value="DUF6230"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19741">
        <property type="entry name" value="DUF6230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666000" evidence="1">
        <location>
            <begin position="43"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="21274" checksum="18614D96D7D5C4B4" modified="2014-01-22" version="1">MKDAQGDPAYGRTNWRRFAVAVAVPTAAAGALVFGMANGAFAATFAVSGQSFKISADKLDGTGFAQYGGSVETADGKSIPVAISGIASAKLYNLCQSVKVPNLPISLTIRAGRDADSPAKATNLLIGVSELSGDAEFTDINIGQDASTLGLGGKDAKGKKGEFGQEAQHVTINGLTQTALSTTAGTFTLTGLNMKINLPGSNGKPPECF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2E3</accession>
    <name>U5W2E3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, TetR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42071">
        <property type="protein sequence ID" value="AGZ42071"/>
        <property type="gene ID" value="AFR_18985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3855"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="184" mass="20236" checksum="A81343F0139AE1B3" modified="2014-01-22" version="1">MEVANRMFLERGYDAVTVAEVAKEAGVSSVTVFKHFPRKEDLFLDRAADAVELLRSAVRDRAPGTDVLTSLRDSTFRLLDARHPLSGIDGRSVPFFRTVAESPALVSRAREIAAELQRTLSEELDDDPAYEGDAALLAAFFLAGYITVLVGTARRLIAGEQADALLDDHRARFERLFEALRGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Q8</accession>
    <name>U5W2Q8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364093.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25750"/>
    <dbReference type="EnsemblBacteria" id="AGZ43414">
        <property type="protein sequence ID" value="AGZ43414"/>
        <property type="gene ID" value="AFR_25750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5220"/>
    <dbReference type="eggNOG" id="COG3981">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113231_3_0_11"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="31"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="177" mass="19772" checksum="87B008237740EAC8" modified="2014-01-22" version="1">MAELITPTHRLHEAWLRSRDEWGRGTHQDGAGLRPGDDVDTADGFAGWVRRLLRESDPAIPAPEGWVHCSYWWIVEDNEVLGAIALRHELNDFLARAGGHIGYGVRPSARRRGLASFALGAVLPYARKLGLDRVLITCNVDNEGSRRTIEANGGVLEDIRDTEIGRLRRYWISLPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W342</accession>
    <name>U5W342_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Simple sugar transport system substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362787.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20715"/>
    <dbReference type="EnsemblBacteria" id="AGZ42415">
        <property type="protein sequence ID" value="AGZ42415"/>
        <property type="gene ID" value="AFR_20715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4200"/>
    <dbReference type="eggNOG" id="COG1879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_3_5_11"/>
    <dbReference type="OrthoDB" id="1378040at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666032" evidence="1">
        <location>
            <begin position="32"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_4" evidence="2">
        <location>
            <begin position="54"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="33879" checksum="815756CF963ADC7C" modified="2014-01-22" version="1">MTLRRTRGARLIALSAVVALGLAACSGGGKEATTDDSGGGANAAGSSGYTIAMITHETPGDTFWDKIKAGAQQAAKDEGVTLKYSNDPDASKQAVLIQNAVDSKVSGIATTLVTPDALAGSVKAATDAKIPVVGFNSGIDQYQQLGALMYFGSDETVAGTAAGKRIADAGGKHPLCVIQAAGSVALEARCAGVKSAVPGTENLQVNGADDGAVVSTLQAKLSQDKSIDYIVTLGAPIAIDALKAMEAASSKAKLITFDLNTEAAQDIQDGKIEFSIDQQPYVQGYMAVSSLYLYLKNGNDVGGGKAVLTGPSFVDKDNIATILPFTKKNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W360</accession>
    <name>U5W360_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362743.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20495"/>
    <dbReference type="EnsemblBacteria" id="AGZ42371">
        <property type="protein sequence ID" value="AGZ42371"/>
        <property type="gene ID" value="AFR_20495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4156"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108054_2_1_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase_6" evidence="1">
        <location>
            <begin position="8"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="13088" checksum="6A0C502676B20EE5" modified="2014-01-22" version="1">MALDLAAINIDAADPDAESTFWHRFLGGSITRTDNHHFLRVDGFPALVIQRAPGHVAPAWPQNGTQQMHVDLTTDDLASADRAALEAGARRLQPVDDADLAHRGSRIYASPAGHPFCLRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W399</accession>
    <name>U5W399_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative myo-inositol catabolism protein IolB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362783.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20695"/>
    <dbReference type="EnsemblBacteria" id="AGZ42411">
        <property type="protein sequence ID" value="AGZ42411"/>
        <property type="gene ID" value="AFR_20695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4196"/>
    <dbReference type="eggNOG" id="COG3718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066438_0_0_11"/>
    <dbReference type="OrthoDB" id="1047507at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008880">
        <property type="term" value="F:glucuronate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019310">
        <property type="term" value="P:inositol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024203">
        <property type="entry name" value="Deoxy-glucuronate_isom_IolB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021120">
        <property type="entry name" value="KduI/IolB_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39193">
        <property type="entry name" value="PTHR39193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04962">
        <property type="entry name" value="KduI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036628">
        <property type="entry name" value="IolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04378">
        <property type="entry name" value="myo_inos_iolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="31850" checksum="8436FE617823AF01" modified="2014-01-22" version="1">MTLLRRGTAGSAPFETVVTPESAGWGYSGLRILDLPIGSRVSFGTGDEEMIVLPLTGGCDVTCDDERVTLTGRRSVFSRVTDFAYLPRDSAVTVRAPNGGRFALPAARADRRLPFRYGPADEVPVELRGAGQASRQVNNFCTPDSFDADRLIAVEVLTPGGNWSSYPPHKHDEPGENETALEEIYYFEVAGSPSGSPGTGFQRVYGHTDHPIDICAEVRAGDVVLIPYGWHGPSMAAPGYDLYYLNVMAGPGERKWLFSDDPEHAWIRGTWSQQEIDPRLPLTSTSERKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3F2</accession>
    <name>U5W3F2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362838.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20970"/>
    <dbReference type="EnsemblBacteria" id="AGZ42466">
        <property type="protein sequence ID" value="AGZ42466"/>
        <property type="gene ID" value="AFR_20970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4251"/>
    <dbReference type="eggNOG" id="ENOG5034BG1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_646607_0_0_11"/>
    <dbReference type="OrthoDB" id="2016316at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="424" mass="46124" checksum="B87E9641DBF4878C" modified="2014-01-22" version="1">MSSALQRLLAVRDFGDAGSSPAVLRRLSLLLGLHTADLFVIAGRPLPADLAPARGSVSRNVGKLVFRASDLLPEHRREVHDFIRSLPVHQPDPEPETGDEVPAGPGPLLVRLLANRNIHPWNAKLLAVVADGPYVSDSTIVLLGQGRVEVTPRYVSAFACLLGIRPEDLAAMTGVEPEPRVRPYPHGAELAALSWDARRLTEDQLLETGHLIEALRRSDPRLFCKQCDRYHVASVHQQTEDTDAGILAAFEQRLDAVLARESPEWTPPQLPAGTDAESVLHHRLDQKRTRAGTHLGLDDLGVIVHDSNASAVRQLVVPLLEAGHGDALRAYLLAVIRTGTSQQQIRAVSAWLWTRPDQPQKAAFQDTCLTAFVAGDDPLVRKVLSRRISLDPSDHAADLHDTVLRARAIAESAPGAYGHLLSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3H3</accession>
    <name>U5W3H3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_26450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364364.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26450"/>
    <dbReference type="EnsemblBacteria" id="AGZ43552">
        <property type="protein sequence ID" value="AGZ43552"/>
        <property type="gene ID" value="AFR_26450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5362"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="19"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="165"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="24427" checksum="FB77925B839AD8FB" modified="2014-01-22" version="1">MTARDRTAAGAAAGARPVRVLLADDQPMLRSGLRMALSGADGLSVVGEAGDGVEAVDLARRLLPDVVVMDVRLPRLDGLGATRAITASGLPVRVLILTAFDTDEHVLAAVGAGAAGYLCKDVPPAELVSAIRVVAGGGSVVAPLILARLLVRLAEVLPAPSTEPVAAVLDVLTEREREVFLHVAKGHSNAEIARALTVSETTVKTHVGHMLTKLGLRDRVQAVVFAYETGLIHPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Z1</accession>
    <name>U5W3Z1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinic semialdehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gabD1</name>
        <name evidence="3" type="ORF">AFR_28360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560270.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28360"/>
    <dbReference type="EnsemblBacteria" id="AGZ43933">
        <property type="protein sequence ID" value="AGZ43933"/>
        <property type="gene ID" value="AFR_28360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5750"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_11"/>
    <dbReference type="OrthoDB" id="384611at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004030">
        <property type="term" value="F:aldehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07100">
        <property type="entry name" value="ALDH_SSADH1_GabD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044148">
        <property type="entry name" value="ALDH_GabD1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="2">
        <location>
            <begin position="3"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="469" mass="49626" checksum="0ADFE9BCE3B45567" modified="2014-01-22" version="1">MPIATINPATGEVLKKFDPLPPEQIDAAIGRSVSGFRALRETSFDQRAAWMRAAADLLEAEQDDVGRLMTLEMGKTLTAAKAEVVKCAKACRFYADNAEGFLADEPADADAVGALRAYARYQPLGPVLAVMPWNFPLWQAMRFAAPALMAGNTGLLKHASNVPQTALYLGELFARAGFPEGAFQTLLVGSDAVEHILSDPRVRAATLTGSENAGRSIAAVAGRELKKTVLELGGSDPFVVLPSADVERAAQVATTSRSQNNGQSCIAAKRFIVHTDVYDRFVDAFVAAMSALTVGDPLLDGTDVGPLATEQGRDDVEAQVADAVAHGASVRCGGQRGDGAGWWYPPTVVTDLTPDMRMYAEEVFGPVAGVYRVESVEEAIKIANGTPYGLGSNVWTSDAPESERFINELDAGAVFVNGMVTSYPQLPFGGVKNSGYGRELSAAGIREFCNLKAVWVGAGDDAGVDSATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W428</accession>
    <name>U5W428_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycine betaine ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_28140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560226.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28140"/>
    <dbReference type="EnsemblBacteria" id="AGZ43889">
        <property type="protein sequence ID" value="AGZ43889"/>
        <property type="gene ID" value="AFR_28140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5704"/>
    <dbReference type="eggNOG" id="COG2113">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072064_0_0_11"/>
    <dbReference type="OrthoDB" id="770506at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007210">
        <property type="entry name" value="ABC_Gly_betaine_transp_sub-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04069">
        <property type="entry name" value="OpuAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666059" evidence="2">
        <location>
            <begin position="26"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="OpuAC" evidence="3">
        <location>
            <begin position="39"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="35121" checksum="2641E2062FE65CE2" modified="2014-01-22" version="1">MLRTRRLAALLSVLALAACGDSATAIVAPPGVERTPCGNVSIAVNPWVGYEANVAVVSYLLKQELGCTVAEKDLTEDESWKGLASGEVDAILENWGHDDLKKTYIDDKKVAVEQGLTGNKGVIGWYVPPWMAKEYPNITKWKNLNEFADRFKTAKSDGKGQLLGGDPSFVTNDAALIKNLKLNYSVVYAGSEDALIAAFRKAEKDKKPLIGYFYTPQWLLSEIDLVHIPLPIYKPGCDADPDTVACDYQPYDLDKIARKAFVDSGSPAADLIKNFRWTNDDQNAVARDITVKKLSRDDAAKRWLDSHRSTWQAWLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W495</accession>
    <name>U5W495_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560370.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28860"/>
    <dbReference type="EnsemblBacteria" id="AGZ44033">
        <property type="protein sequence ID" value="AGZ44033"/>
        <property type="gene ID" value="AFR_28860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5850"/>
    <dbReference type="eggNOG" id="ENOG50339E1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156558_0_0_11"/>
    <dbReference type="OrthoDB" id="1811115at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="103" mass="11214" checksum="B8FEC2CF2BA293C8" modified="2014-01-22" version="1">MITQLTGRSYRFDLGDLLVRFTFDSPTQGSFVVVQGAGLAPDGHAETVALNLQEIREGVFLNSWTEASGATVTHLEDFENGKLYSNVTVDGTLHHFVGTIKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4B7</accession>
    <name>U5W4B7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22800"/>
    <dbReference type="EnsemblBacteria" id="AGZ42830">
        <property type="protein sequence ID" value="AGZ42830"/>
        <property type="gene ID" value="AFR_22800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4621"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="34106" checksum="23F665999B5300CF" modified="2014-01-22" version="1">MSLMIFVHGWPELGLVWRAQAEHFTAAGWRCVTPDLRGYGTAPAPDDTSAYAVEHVVADLIALHDSLGGEPAVWVGHDWGSPVVWALAAHHPERVRAVASLCVPYLPQGFALETLVPLVDRDLYPAERFPYGQWDYYRYYAESFEQVTADFEADVEATVSLLFRRGRPESVQRPARSAGVRANGGWFGAARRAPQLPRDETMLSAEDHATIVASLSAKGFRGPGAWYLNDDANVAYAARAAYPELRMPVLFVHAAWDAICETRGSRLAEPMRAACTDLTETTIDAGHEVMLEAPAETTTALATWLAAAGLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4W7</accession>
    <name>U5W4W7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363576.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23590"/>
    <dbReference type="EnsemblBacteria" id="AGZ42986">
        <property type="protein sequence ID" value="AGZ42986"/>
        <property type="gene ID" value="AFR_23590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4780"/>
    <dbReference type="HOGENOM" id="CLU_2911997_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="61" mass="6215" checksum="70C392F50C9D96B0" modified="2014-01-22" version="1">MDTTLLRLAAELRKAAADAGRSDVVAKVDEVIALLAGDRVAGEGSAPKTNAIIKLLDGLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W610</accession>
    <name>U5W610_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xaa-Pro dipeptidyl-peptidase</fullName>
            <ecNumber evidence="3">3.4.14.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">X-prolyl-dipeptidyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_31765</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes Xaa-Pro-|- bonds to release unblocked, N-terminal dipeptides from substrates including Ala-Pro-|-p-nitroanilide and (sequentially) Tyr-Pro-|-Phe-Pro-|-Gly-Pro-|-Ile.</text>
            <dbReference type="EC" id="3.4.14.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S15 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.11" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560946.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31765"/>
    <dbReference type="EnsemblBacteria" id="AGZ44613">
        <property type="protein sequence ID" value="AGZ44613"/>
        <property type="gene ID" value="AFR_31765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6430"/>
    <dbReference type="eggNOG" id="COG2936">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011800_1_0_11"/>
    <dbReference type="OrthoDB" id="327988at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005674">
        <property type="entry name" value="CocE/Ser_esterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008252">
        <property type="entry name" value="Pept_S15_Xpro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000383">
        <property type="entry name" value="Xaa-Pro-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013736">
        <property type="entry name" value="Xaa-Pro_dipept_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02129">
        <property type="entry name" value="Peptidase_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08530">
        <property type="entry name" value="PepX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00923">
        <property type="entry name" value="LACTOPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00939">
        <property type="entry name" value="PepX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00976">
        <property type="entry name" value="NonD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666125" description="Xaa-Pro dipeptidyl-peptidase" evidence="9">
        <location>
            <begin position="29"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="PepX_C" evidence="10">
        <location>
            <begin position="347"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ44613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="624" mass="66657" checksum="3A7EB3AC4FDDFB8B" modified="2014-01-22" version="1">MPVAVPRALLVALVVLGGFLTAPPPASAAPPFVRGGETVPVYDYADAIRESVWVQTPTDADEDGVPDRVAVDLVRPRTAPGVKVPVIMDASPYYACCGRGNEGELKEYDENGVISKEPLFYDNYFVPRGYAFAGVDLSGTNRSTGCEDVGGPAEVAGAKAAIDWLNGRAPGFTADGTPVSARAWSTGKVGMIGKSWDGSIANGVAATGVRGLETTVPIAAISSWYDYMRYNGSLRSSGYPGYLQGVVSDRIAECAAVTASLANDSADETGDYNAFWRARDYRPSAGQVHASVFVVHGVNDLNVTTPQFAEWWQDLAAHHVPRKIWLHKTGHVDPFDLRRAEWVDTLHQWFDFWLQGLPNGVMRAPRATVETAPGTFTSARDWPLPGTRPVSARLGSSVVSYTDESLSEDAAVASPSTEVPGRIALLYGPLTSPLRLSGTPSVRLRVQVDRPTTQLTARLVDYGTATRVDYGRGEGVRTLATESCWGASTPADDACYLDVAERTATADHEVLTRGWLDAAHHRSLRFTTPLQPGRWYTVTVPISASDTTVAAGHTLGLVLTQSDPEFTEAANTGATVKVDLATSRLTLPISGKATLQPAPAGTVVTTAPIAPAERKAQPRLQLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W645</accession>
    <name>U5W645_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rok family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31965</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31965"/>
    <dbReference type="EnsemblBacteria" id="AGZ44653">
        <property type="protein sequence ID" value="AGZ44653"/>
        <property type="gene ID" value="AFR_31965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6470"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="412" mass="42334" checksum="1EFF13E698AECB52" modified="2014-01-22" version="1">MRDVRRANRASLLTDLFHGGRQSRQQLGDTTALSQASVSNLIGEMIDEGLVEEAGLVGSDGGRPRALLRVAPGYGYVVGADVGETRVLVELYDLAMSRLGVAQYSLGTGGPDPEVAAAKLLEGLDSVVAEAGIARSEVLGYGVGVAGVVDQTGGSPVVHSQTTGWDAVPLGDMLAAGTDVQIFVENGAKTVGQAEMWFGAGRGARHAVIVMVGTGVGAAVVMDGRSYRGAFSNAGEWGHTTLVYDGDVCRCGARGCLEAYIGAEKVIARLAAATGQQPEPKLLSRMLAADGALEGEAADIIAETVGYLGAGIAGLVNLFSPERIVLGGPAGLALGERFLPDIRAAAARNALKQPYSRTRIDLCQLGPDAVAMGAATLPIARLLADGGLPVTSGQAAPLSRAAHRRSMSRGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W660</accession>
    <name>U5W660_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364241.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26140"/>
    <dbReference type="EnsemblBacteria" id="AGZ43490">
        <property type="protein sequence ID" value="AGZ43490"/>
        <property type="gene ID" value="AFR_26140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5298"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079334_0_0_11"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="29954" checksum="A637A6F151947BD7" modified="2014-01-22" version="1">MAQRLLITGGSGKVATSLRPRLAAPGRTLRLLDIVAPAPVDGSGDEEVVIASVDDLDALTAAFADVDAIVHLGGQSKESDAEDVLRLNAYGTYCVLEAARRAGVKRVVLASSSHAVGFHERDDSYPDGLPADLPPRPDTLYGWSKTAVESLGRLYVDRFGLDVICLRIGLWFPTPPQLRGLALWLSPDDGARLVEACVAVEHPGYRIVWGVSRNTRRWWSLAEGEAIGYFPKDDAEIFAERYVAEFGEPDFEHDPLLNRVGSYWCDHPLGQRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6G5</accession>
    <name>U5W6G5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetylglucosamine 4-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31635"/>
    <dbReference type="EnsemblBacteria" id="AGZ44587">
        <property type="protein sequence ID" value="AGZ44587"/>
        <property type="gene ID" value="AFR_31635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6403"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="27403" checksum="2BF800D3497BED14" modified="2014-01-22" version="1">MGRRPGPVGRHVAWDAAREVPDLDGVDAVVHLAAAVGDPRPGRATEAAFHAVNVGGTARLLEAAAGRPVVWVSSASVYRPGPYREPVREDHPVGGQRGAYGRSKAAGERLALAAGTVVLRPRAVYGDGDRHLLPRLRRVVRRGTAWLPGPDVAMSLTAVENLAGACLAALTWPAGAYNIADDRAYRRDEVFGLVLGVPVRHVPAGLARVAAVVSPTLTRYAVDQVTDGLVLDLGRARAQGYRPVRTLADYLVVTTDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6L2</accession>
    <name>U5W6L2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_32975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561190.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32975"/>
    <dbReference type="EnsemblBacteria" id="AGZ44853">
        <property type="protein sequence ID" value="AGZ44853"/>
        <property type="gene ID" value="AFR_32975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6673"/>
    <dbReference type="eggNOG" id="COG3476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091805_2_0_11"/>
    <dbReference type="OrthoDB" id="1679387at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PTHR10057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005859">
        <property type="entry name" value="PBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="160" mass="17773" checksum="E5B3FF501FC9E5D2" modified="2014-01-22" version="1">MTSHVLPRSRSSWWGLIPFAVAVAVAALIGGLGVAGTASEYQSLEQPSWAPPSWLFGPVWTTLYAMIAVAGWLVWRRTGWTTALNVYAVQLVLNAVWTPIFFGFGRYGLALVDIVVLWLLIGVTIYLFRPISRPAAWLLVPYWAWVTFATALNAAIWHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Y4</accession>
    <name>U5W7Y4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561664.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35360"/>
    <dbReference type="EnsemblBacteria" id="AGZ45328">
        <property type="protein sequence ID" value="AGZ45328"/>
        <property type="gene ID" value="AFR_35360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7155"/>
    <dbReference type="eggNOG" id="ENOG5033AAH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120892_2_0_11"/>
    <dbReference type="OrthoDB" id="1978329at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021449">
        <property type="entry name" value="DUF3099"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11298">
        <property type="entry name" value="DUF3099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="113" mass="12521" checksum="3FF4F34FBEF9405A" modified="2014-01-22" version="1">MKKQPVLITNAARSQDDQLRSRQIRYVSMMSVRAGCLILGAILISVKPPFLPLWLILCAAGMVFLPWAAVLIANDRPPKSRAHPAGVPTVNNQRILTAESAEEVRHRTIDLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W819</accession>
    <name>U5W819_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SMI1_KNR4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561494.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45157">
        <property type="protein sequence ID" value="AGZ45157"/>
        <property type="gene ID" value="AFR_34495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6979"/>
    <dbReference type="eggNOG" id="ENOG5033DBY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1774334_0_0_11"/>
    <dbReference type="OrthoDB" id="1776264at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09346">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SSF160631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMI1_KNR4" evidence="1">
        <location>
            <begin position="14"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="15356" checksum="E2B74613F92240AB" modified="2014-01-22" version="1">MTNWVDGAIDDPRPASDTAVRQAEDELEVSLPSEFLAVARVHQGAAPVPAGIDLPNGFSTAVDHLLHFEDSPFVSNIVAAIFPLLDVLDEGIIPFAADVGGDFFCFDYRADTNHPSIAFWSVDWGVVPLAPNFATFLDMLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W828</accession>
    <name>U5W828_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29320"/>
    <dbReference type="EnsemblBacteria" id="AGZ44125">
        <property type="protein sequence ID" value="AGZ44125"/>
        <property type="gene ID" value="AFR_29320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5941"/>
    <dbReference type="eggNOG" id="COG4977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="81"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="19821" checksum="F5979C44E8A0C220" modified="2014-01-22" version="1">MTATDPAGRAPELHLEKTTGSPPPPDEHVLRVMVARLQHLAASTQAGDLSATTAAGLLAVHVLTCHGTAPPPPPRHDVRVQRVIELLHANVHTALKLADIAAVAGLSRFHLLRLFQQQTGETPARFHLRLRIELGQGLLRTTRVSITEIAGLCGFVDPSHFSKTFRRHTGMSPSRYRAALRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W862</accession>
    <name>U5W862_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560497.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29495"/>
    <dbReference type="EnsemblBacteria" id="AGZ44160">
        <property type="protein sequence ID" value="AGZ44160"/>
        <property type="gene ID" value="AFR_29495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5978"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141550_1_0_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="7"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="12943" checksum="F02328981F1B9CB3" modified="2014-01-22" version="1">MTSPFTTVADVFCFVPDLAAATAWYTDRLGVPPALQAAQLARFDLGTSSLTLHQFDEFNTGGTAGCVAYWDVEDVDAVVAEWGRHGAIAHRGPKTIMTGERLCQLLDPFGNLIGVRQASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8L7</accession>
    <name>U5W8L7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="1">4.2.1.136</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ADP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nnrD</name>
        <name evidence="2" type="ORF">AFR_30080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560613.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30080"/>
    <dbReference type="EnsemblBacteria" id="AGZ44276">
        <property type="protein sequence ID" value="AGZ44276"/>
        <property type="gene ID" value="AFR_30080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6093"/>
    <dbReference type="eggNOG" id="COG0063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_2_2_11"/>
    <dbReference type="OrthoDB" id="1748633at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="28762" checksum="0F9B8A9BB46591BB" modified="2014-01-22" version="1">MPSPSETERVITPQLLRDWPLPNPQGGKLARGTVLVVGGSRFTPGAALLAGVGALRAGAGRLQLAVESSTASALSIAVPEALVIGLPENEDELSRLAGEADVVAVGPGLNDIEGTARLLHRVLDVAAKDTAIVLDAYALGALSKEPDLLAGRSQGFVLTPNVTEAEHLLGREPSDDLAAEARELSDRYAATVSLFGHIAAPDGRAWREESGDAGLGTSGSGDVRAGIVAGLLGRGAEPAQAACWGAFAHAVSGQRLAPRHGRTGFLARELVDEVASTIATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8R5</accession>
    <name>U5W8R5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">rpmF</name>
        <name evidence="7" type="ORF">AFR_36535</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561899.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36535"/>
    <dbReference type="EnsemblBacteria" id="AGZ45563">
        <property type="protein sequence ID" value="AGZ45563"/>
        <property type="gene ID" value="AFR_36535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7391"/>
    <dbReference type="eggNOG" id="COG0333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129084_1_1_11"/>
    <dbReference type="OrthoDB" id="2092354at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00340">
        <property type="entry name" value="Ribosomal_L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_L32p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665809" description="50S ribosomal protein L32" evidence="6">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="57" mass="6233" checksum="00E9820B84679CD6" modified="2014-01-22" version="1">MAVPKRKMSRSNTRSRRANWKATAVSTVACPQCKSPKLPHTACGVCGTYNGRQVLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8W8</accession>
    <name>U5W8W8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561798.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45462">
        <property type="protein sequence ID" value="AGZ45462"/>
        <property type="gene ID" value="AFR_36030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7293"/>
    <dbReference type="eggNOG" id="ENOG5033GCQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1515989_0_0_11"/>
    <dbReference type="OrthoDB" id="1773064at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="37"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="20407" checksum="0FA9AB8D2979B06B" modified="2014-01-22" version="1">MTTTGLERALNESSLRPLPATAAELLREVGAPPRLGAHLRAVHDVAWSLTDNLGRRRPDLEFDTTAVLFGAATHDIGKVVHVRELSAPGHAHEEAGRDLLLRYGIPAHLARFAGSHGSWGADAPLDDLLVSLADKVWKAARVEDLEQHVATYLGGAPWQAFMELDDLLQELAAGADERLAFQASYPIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W952</accession>
    <name>U5W952_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560853.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31300"/>
    <dbReference type="EnsemblBacteria" id="AGZ44520">
        <property type="protein sequence ID" value="AGZ44520"/>
        <property type="gene ID" value="AFR_31300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6335"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="138"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="207" mass="22261" checksum="AD3A290E4B74526F" modified="2014-01-22" version="1">MIGLLIVDDHPVVRDGLRGMFAGDERFEVLGEAADGTEALAVARRVRPDVVLMDLRMPGMDGVTTIRALREQGFEARVLVLTTYDTDSDVLPAIKAGATGYLLKDTPREELFRAVSAAHRGESVLSPAVASRLMGELRSPAREPLSQRELEVLGLIAQGCSNRETAGRLFISEATVKTHLLHAYAKLGVRDRAAAVAAAFERGLLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9A4</accession>
    <name>U5W9A4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561923.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36655"/>
    <dbReference type="EnsemblBacteria" id="AGZ45587">
        <property type="protein sequence ID" value="AGZ45587"/>
        <property type="gene ID" value="AFR_36655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7415"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_4_1_11"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="2">
        <location>
            <begin position="41"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="26625" checksum="2ADC29194166A862" modified="2014-01-22" version="1">MARRKLGFWRRLAVLLVLPALRVWTKRTWSGMENLPAGGGFIITPNHMSQFDPLLVAHFVYDSGRWPRFLAKASIWKVPFVGFMLRKTQQIPVERGSVEAVKSLESLIEALQQGGVVVIYPEGTTTREPGLWPMRGKTGAARLALVTGAPVVPMATWGAQQVHDVRTGKISLGRRIPVSVTAGKPVDLNRWLGQAPGRAVLEQMTEAIQLATRDLLAELRDEPPPPLYPVSRRPAGSSEPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9A9</accession>
    <name>U5W9A9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31355"/>
    <dbReference type="EnsemblBacteria" id="AGZ44531">
        <property type="protein sequence ID" value="AGZ44531"/>
        <property type="gene ID" value="AFR_31355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6346"/>
    <dbReference type="eggNOG" id="COG3087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091587_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="23491" checksum="B5C1C59FEA4A95D1" modified="2014-01-22" version="1">MGFWTAGCSSATTLGARQMPASDEPQEHVLAGALHGRTGAFLIVRDSASRVEVRLADLPGLLYRISTPADSGLAPAVTGTQGRVRLGLRPTGGDGPDTVEILLNRAVRWDIRLPAGAGEQHLDLADGRIRRLELGSGTGLVSMRLPPPRGTVGIKLAGGVGEIAVAMPPQTALRFHLRGGADLVAVPWAGRERATAGAMIAPAGWSAARNRYAVDVEAEVGTVTVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9F1</accession>
    <name>U5W9F1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative multi-sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31700</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31700"/>
    <dbReference type="EnsemblBacteria" id="AGZ44600">
        <property type="protein sequence ID" value="AGZ44600"/>
        <property type="gene ID" value="AFR_31700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6416"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_44_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="151"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="4">
        <location>
            <begin position="229"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="456"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="675" mass="72985" checksum="D7A86727BB017A81" modified="2014-01-22" version="1">MLTSGAADQLAALIARAARAPAGLVHFVEGERLRIYGGYGLAVRWEDVADTQLKDTLAGLLIETGEPLVIGNLATDPRVPPGSPIRAHGMTGAYLGYPVRDDTGTIVGICCALDTEPHEWTDNDVAAVAEAAHVSTLLITEQIARLEIDRQRRFLDAVVDSLHDGVTACDADGNIVLVNARMQKLWGGAQVPGGLDDEAAIAGLTDADGNPIRRDDIGLFRALRGERLRDEELVLQGRGRRTRYYLVDAQPILGPDGRTVGAVQAVQDVTRRRRAERFRTCELAVVTALAAARTVEAAGPRVLEAVVRTLGWTHAELWLVDEPAGVIRSAASWHSPSWQADIDVPEELPFGTGLPGRAWQAGKPLWIRDVGRPQSLISPETAASSKLHTALAIPVRNGLEPLGVLVAFADTIEDPEDELVALMSGISAQIAQFIDRRVVEDLTLQLTRSKNEYLALVGHELRTPLTSIAAYTELLRDADADTLIKDGPRLLEVIDRNSTQLRHIINELLELSALDTGHASVQLTPVNLAEVVRESVQGTRDAVADEPVTLVDDLPAELVLPGDRKRLRQVLDNLLGNAVKYSPDGGRIKVTLRTVGRTAELAVSDTGIGVSQEEREKMFTGLYRTSRARDRAIPGSGLGLTLSRAVVERHHGTIELTDHEGPGTTVLVRLPLDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9V1</accession>
    <name>U5W9V1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38325"/>
    <dbReference type="EnsemblBacteria" id="AGZ45919">
        <property type="protein sequence ID" value="AGZ45919"/>
        <property type="gene ID" value="AFR_38325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7753"/>
    <dbReference type="eggNOG" id="COG1451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094108_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002725">
        <property type="entry name" value="YgjP-like_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01863">
        <property type="entry name" value="DUF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF45" evidence="1">
        <location>
            <begin position="60"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="19571" checksum="219C7B00ED59C885" modified="2014-01-22" version="1">MRRSQRRRRTVSAYREGERVVVLIPDRFSRAEETEWVARMLARLAAREERIRRTDSELLVRARRLISRYLPDHADEIVPASVRWVTNQNGRWGSCTPDDGTIRISHRIQEMPDWVIDYVLLHELTHLVVPSHNTKFWQLVNRYPKAERARGYLEGISAATGLVLADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA33</accession>
    <name>U5WA33_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38750"/>
    <dbReference type="EnsemblBacteria" id="AGZ46004">
        <property type="protein sequence ID" value="AGZ46004"/>
        <property type="gene ID" value="AFR_38750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7841"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048109_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="154"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="30613" checksum="8F0D73FF80B2F57D" modified="2014-01-22" version="1">MTETELTLATAKGTLRVPLAEVHRAKRVPAARRPPAADVIALELAANDAWPAPIQARLGGWILRAADNWTGRANAALAVGDPDRTLGAAIDAVEQWYAAKGQRPMINTPMPLAAPVNAALDARGWTARPLVLVQTGPVQPLLTEVAARRDLPPVELADSPTDAWYAMVAAHKGALPATARQIITGVPDVVFASVQDETGNLLAVARGAVTGPDRWLGLSLVQTAPSARRRGLGRHVMRAVAQWATQRGAARAYLQVEENNAGAVGLYQSLGFSTHHTYLTREAPEAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAC7</accession>
    <name>U5WAC7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562477.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39445"/>
    <dbReference type="EnsemblBacteria" id="AGZ46143">
        <property type="protein sequence ID" value="AGZ46143"/>
        <property type="gene ID" value="AFR_39445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7980"/>
    <dbReference type="eggNOG" id="COG2220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091682_0_0_11"/>
    <dbReference type="OrthoDB" id="767622at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="7"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="22511" checksum="68F63A730071D349" modified="2014-01-22" version="1">MRVTKFTHSCLRLEGDGAVLVVDPGAFSERSALTGADAVLITHEHFDHLDVDGLADALARHPGLRVYAHPDVLPKLTAFSDVVTAVEPGTSFTAAGFAVRAYGGEHAIIHADIPQIANLGFLISDGTGSVYTPGDSFTVPDEHVETLFVPLNAPWMKLSEAIDFVRAVRPERAFALHDHLLTETGAQVSDGHLERLGGTKYAHVAPGTTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAG6</accession>
    <name>U5WAG6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PhoH-like ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_39250</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562438.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39250"/>
    <dbReference type="EnsemblBacteria" id="AGZ46104">
        <property type="protein sequence ID" value="AGZ46104"/>
        <property type="gene ID" value="AFR_39250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7942"/>
    <dbReference type="eggNOG" id="COG1875">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022283_2_1_11"/>
    <dbReference type="OrthoDB" id="445854at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PINc" evidence="7">
        <location>
            <begin position="44"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="470" mass="51218" checksum="4B12215772B90E96" modified="2014-01-22" version="1">MTSRRNDAGASQDPAANVSSSRASTGRRSSRDRHADAEPAPAGRVFVLDTSVLLSDPAAFRRFTDHEVIIPLVVISELEDKRHHPELGWFARQSLRMLDELRIKFGRLDQPVLCNDEGGTMRVELNHADQSVLPQGFRNDSNDTRILSVALGLAAEGRDVTLVSKDMPLRVKAASVGLTADEYRHGQASDPTWVGMADLKISEDQVSQLYAGEELELEEADALPCHTGLVIHSSRGSALGRVNPDKSVRLVRGDREAFGLRGRSAEQRIALDLLLDESIGIVSLGGRAGTGKSALALCAGLEATMERNRHKKVIVFRPLYAVGGQELGYLPGSESEKMSPWAQAVFDTLGAVVHANVMEEVMARGMLEVLPLTHIRGRSLHDAFVIVDEAQSLERNVLLTVLSRIGQGSRVVLTHDVAQRDNLRVGRHDGVTAVIEALKGHPIFAHVTLTRSERSPIAEVVTDLLEDIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAJ6</accession>
    <name>U5WAJ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39450"/>
    <dbReference type="EnsemblBacteria" id="AGZ46144">
        <property type="protein sequence ID" value="AGZ46144"/>
        <property type="gene ID" value="AFR_39450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7981"/>
    <dbReference type="eggNOG" id="COG1579">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046535_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025645">
        <property type="entry name" value="DUF4349"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14257">
        <property type="entry name" value="DUF4349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="37933" checksum="43E33D7AF1B84181" modified="2014-01-22" version="1">MTLALAGCGDSSDTQSSSGSTADRAGPAAEAPAAGGADEADSNAGAAPEQGKDQAAQAPDLAVDQRSIIYTGSITVRVDDVNIAAARASGIATAAGGFIGGDKRNSNSGSSDATLELRVPADKFSSAVDQLAKLGTEENRGINTEDVTEQTIDLDARIATQTARVDSGRKLLGQAKSLSDLVMLEREVATRESDLASLQAKKRRLADLTSLSTITVILLDPQAAAAQEEESAPGFLSGLENGWKGLLASLGVLLTVIGWLLPWLLAIGLPAWAILWAYRRYRATRPVRPIPPIPAYATPHPGFPPYPPQPAPQSAPPASRSAPQSAPPAQQSAPPATRSAPQSTPPSQQSAPPAPPPAPSAGPDQKASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAM6</accession>
    <name>U5WAM6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminopeptidase N</fullName>
            <ecNumber evidence="4">3.4.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AFR_33740</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.</text>
            <dbReference type="EC" id="3.4.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.2" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561343.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33740"/>
    <dbReference type="EnsemblBacteria" id="AGZ45006">
        <property type="protein sequence ID" value="AGZ45006"/>
        <property type="gene ID" value="AFR_33740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6829"/>
    <dbReference type="eggNOG" id="COG0308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014298_2_0_11"/>
    <dbReference type="OrthoDB" id="771837at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="SSF63737"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665724" description="Aminopeptidase N" evidence="12">
        <location>
            <begin position="25"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M1_N" evidence="14">
        <location>
            <begin position="54"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M1" evidence="13">
        <location>
            <begin position="314"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="466"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ45006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="515" mass="56372" checksum="EAB052DAEDF665AD" modified="2014-01-22" version="1">MRRKLLCAVLAGGLAITPGGPAGAAPARDYRPGSAGLGDVWYPLEGNGGYDVAHYDLSLSYDPGSGRLDGTARVDAVATQNLSRFDLDLQQLDVTRVLVDGRPASFRRDGQELVITPRRGIPARSRFQVAVTYGGVPQPLGGPTPYGFVRTGDGMVTLNVQDGASTWFPGNDHPSDKATFRFTITVPDGLDVVANGRRVSTRTHGGRTTHVWSETAPMATYLATVDVGRWRVRKGRTPGGIPEYVAVDPALGTGADDPMPQFWNRTAEVVDRWTQIFGPYPFDSTVAIVVLARHNGVQVPFSAETQTRPVYSAADSDEIIAHELAHQWFGDSVGITRWNEVWLSEGFAVFANWYFDELRGGTTVHDQARQVYQAHPADDPFWTIPLTGPDRPTDAAGERIYFGGALVLQMLRQELGDPTFFRVLRAWTRDHRHGTATTTDFMETATRTSGRDLAPFFRTWVYSPTRPALPDNAEQPRSSVAHDGAERQPFPRHRQSSSDAPSPGLHPEGTPPGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAW2</accession>
    <name>U5WAW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cysteinyl-tRNA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_34260</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561447.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34260"/>
    <dbReference type="EnsemblBacteria" id="AGZ45110">
        <property type="protein sequence ID" value="AGZ45110"/>
        <property type="gene ID" value="AFR_34260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6932"/>
    <dbReference type="eggNOG" id="COG0215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046644_0_0_11"/>
    <dbReference type="OrthoDB" id="1601610at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="467" mass="50074" checksum="FA9F240ED15126CE" modified="2014-01-22" version="1">MSLLVIMGSGETSPTMVSVHRRLAGTLPSRPSAVLLETPYGFQENVADISAKACAYFARSVGLSVTVAPGLRGPGVAAETDQGLAAVRAADWLFSGPGSPTYAMDCWRSTPVGQALQDRLARGRRPNFNEVGPSRPDRPNFDEVGPSRQRPNFNEVGPSRPDRPNFDEVGPSRQRPNIKEVGPVTVLASAAAATMGRWAVPVYEIYKVGATPYWAQGLDLMAHLDLSVAVIPHYDNTEGGTHDTRFCYLGERRLRILERELPAQAAVLGIDEHTAALIDPPNDSVEIVGRGGVTVRRAGADVVLPSGTVLSLTRLRSLVRGEVRGGAPKVEQREELEQPPTVSEVAADCEREFDEALARQDAAGLGQAVLDLESAVRAWSADTEEDDGADQAAAVLRTLIVRLAQVAELDPAERLAPLVDPLIAWRARLRAAGDFAGADEVRSLLAEAGVLLRDTPGDTRWTLPAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAW9</accession>
    <name>U5WAW9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Putative molybdopterin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_34065</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561408.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34065"/>
    <dbReference type="EnsemblBacteria" id="AGZ45071">
        <property type="protein sequence ID" value="AGZ45071"/>
        <property type="gene ID" value="AFR_34065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6893"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_4_11"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02791">
        <property type="entry name" value="MopB_CT_Nitrate-R-NapA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041957">
        <property type="entry name" value="CT_Nitrate-R-NapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="8" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="11">
        <location>
            <begin position="39"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ45071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="768" mass="84241" checksum="E0A29600DD7DF038" modified="2014-01-22" version="1">MADRIADPWGERTPYGPGQTWPARVDLHLAEGVEEADVDRWVQSASILHSNGDAMDIAVKDGRIVGVRGRAEDRVNRGRLGPKDLYGWQANNSPDRLTRPLIRQDGRLVETDWDTAMGRIVHRSRELLEGPGGWGHFGFYTSGQLFLEDYYTLGLIGKAGLGNPHMDGNTRLCTATAAAALKASFGTDGQPGSYTDVDHCDTIAVWGHNPAETQAVLWMRMLDRRSGPNPPALLAVDPRSTPVAREADVHLAPRTGTNQALLNGLLREIIQRGWCDDTYLAAHTIGFDELSRVVDACPIDRVAGICDLKAADIERAAEVIGTAGRLLSTVLQGFYQSNQATAASCQINNLHLVRGQLGRPGAGVYQMNGQPTAQNTRETGADGDLPGLRNWDNPDHIRELAELWNVDDLTIPHWSPPTHALQIWRYAEQGSIKLLWISATNPAVSLPDLARIRRILAQPELLVVVQDLFLTETAELADVVLPAATWGEKTGTFTNADRTVHLSEKAVDPPGEARADLDIFLDYARRMDFRNRDGEPLIGWTTPEEAFEAWKWCSAGRPCDYTAITYERLRGGSGIQWGGERLYTDGVFNTDPGYCETYGQDLSTGAEFTAEQYRAKEPAGRAFLHAADYQPSPEVPSGDFPLLLTTGRTIYQFHTRTKTGRAPELDAAAPDVWVELHPDDAARYGITEGDLVNISSPRGGIEARARLGGIRLGVVFVPFHYGYFDVDPAGRRPRAANELTVTAWDPVSKQPIFKVAAVRIAKLAGPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBK9</accession>
    <name>U5WBK9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562885.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41495"/>
    <dbReference type="EnsemblBacteria" id="AGZ46553">
        <property type="protein sequence ID" value="AGZ46553"/>
        <property type="gene ID" value="AFR_41495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8396"/>
    <dbReference type="eggNOG" id="ENOG5033JK8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1472235_0_0_11"/>
    <dbReference type="OrthoDB" id="1808686at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="19407" checksum="44C9A515019B3ED9" modified="2014-01-22" version="1">MPRILERSLTGLFRSRWGVAVVIAVLVLAVVGIGRLFSGTNPQPPLTDAVPTAPTVSVDPSHDDDGVIVDDEPPPEPSTRPGTAKPEAVAYAFASAWVDHKNVSAKTWHNALLPNATKDLADELDGVDPADVPADRVVDRPTLVPLGDGLVNAVITVDSGKLTLQMVAPDGRWLVDGVDWNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBV1</accession>
    <name>U5WBV1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PAP2_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_35900</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561772.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35900"/>
    <dbReference type="EnsemblBacteria" id="AGZ45436">
        <property type="protein sequence ID" value="AGZ45436"/>
        <property type="gene ID" value="AFR_35900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7266"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052628_1_0_11"/>
    <dbReference type="OrthoDB" id="1742143at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="PAP2_3" evidence="6">
        <location>
            <begin position="111"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="300" mass="34390" checksum="399F07489EC5368E" modified="2014-01-22" version="1">MLIWAVLFAVGTYFIGVPTSDPLIAFGWLWLATIAWRSDQPWRSHLLFLRDWLPISLLLVVYNISRGYADDLFAPHVHELIDADEAMFGWFTGGEVPTVWLQQHLYQPNVVQWWEVAVSLVYVSHFLAVPTVGVILWLRWREQWGRFMRRWFTLSVAGLITYFLYPAAPPWWASLYGQLPEHVERISTNGWNAVGLHSAGNTLNALQVEASNPVAAMPSLHTAYALMAVAFFLPMVAKRWWPLLLAYPLAMTFTLVYSGEHYVIDVLVGWAYVGGVFLVVGFAERWWRSRRAVEPADPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC22</accession>
    <name>U5WC22_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative 5'-nucleotidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_42125</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42125"/>
    <dbReference type="EnsemblBacteria" id="AGZ46679">
        <property type="protein sequence ID" value="AGZ46679"/>
        <property type="gene ID" value="AFR_42125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8524"/>
    <dbReference type="eggNOG" id="COG0737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2374">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003555_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="SSF55816"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666269" evidence="2">
        <location>
            <begin position="33"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="domain" description="LTD" evidence="4">
        <location>
            <begin position="26"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="3">
        <location>
            <begin position="1347"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="3">
        <location>
            <begin position="1437"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1607" mass="165617" checksum="7046AB02D2DC1992" modified="2014-01-22" version="1">MRERYNRSALRAVGAAVVATALGLSFTGPVNAAAKAAETPFISEIHYDNAGADSGEAIEIQAPAGFDLSGWQIVLYNGSNGAAYNTVTLSGVVPAAGVVVQAYPVNGVQNGPPDGVALVDAAGGVAEFLTYEGPMTATGGPAAGMTGVDIGVSEPDSTPVGSSLQKIDGVWKGPAANSFGKLNAVVTDPDPDPDPPVGCTTAPDHTIAQVQGTGTATELAGTRVTVEGVVTADHRAGGYNGVYVQTAGSGGDRPVAAGTASDGIFVYLTTDAAVHPAVAIGDLVRVSGTPTEFNGLTQLAIAAKADVQICSHGAPLPAPVALSLPLADDARESAESMLVAPVGAYSVSDVFNTNRFGEVVLAAGNAPARVPTDVARPGTDAAAQVKAANKLGRILLDDGRTTNLSSAGLAPPYLSKDDPVRVGDTVEAFAPSVLGFGFSEWRLQPTLPVAADTPATGRTTFKDTNPRTPGPADVGGDIRLASFNVLNYFVHFGGEARGATTPEALAKQQAKIVSAITALDADVVALMEIENSVRFNTEDPQQALKTLVGALNEADGAGTWDYVRSPAELPAAAQQDFITTAIIFKPAKVTPKGAARSINDETVWSNAREPIAQAFTSGSITFTVVANHFKSKSASTTPTGDNVDTGDGQGPFNGDRVRQAKSLATFVDDLKTSSGSDQVLLLGDFNAYTQEDPLQALYDKGFTDVHSTYAQGKYSYVFSGETGSLDHGLATASMAQRVTGVDIWNINSVESFAFQYDGFAPFYDAGPYRASDHDPVVVGLDTGAPKPVDLQLLSINDFHGRLESPATVDGKPVGGAAQLVGMVNQLRAANPNTAWVSAGDNIGASTFISAIDGDNPTIDALNAGHLNVSAVGNHEFDKGLADLLGRVETRADFPYLAANVYKDDKRVLPGYVVQSLGGVRVGYIGVVTEQTGSLVSPDGIAGVQFRDPVAEANTVASQLSDGNDTNGEADVLVLLAHEGAASENIGSAADLVADPVFGEFTQVSADIDAIFSGHTHQPYAFQVPIPGTDRTRPVIQAEDYGVKLGKAVLTYDPATDSVTASTAELLPVVGATPDPTVAGIVATAKANATELGKQPLGKITADIKRGYTGGTENRGIESVMGNFIADVQLDQTSAAGRGGAQIAFMNPGGLRADLVYGTDGTVTYADAFSVQPFANDVVTQTLTGAKIKQVLEEQWQPDGASRPVLHLGVSKGLTYSYDAAQPRGQRIIASSLELNGVTLNPTGTYRVTSNSFLASGGDNFTTLGTGTDRKTTGDNDLTMLVNYFAAHSPVTADPAPRSTAGVLDGTAPTGTFALNTAAIWPGQSVQLTQTALADNVSTPAGIRRVITWGDGSAPQTLAAGATSAAHAYAKVGSYQVSVALTDEAGNTGPATLSSATVVVAAQPGRYTVDRTSIWATQSVKLSLTGLTGATKVEVAWGDGAITTMSPNVTSAAHSYTKAGTFKIKVTPSNAAGAGAPVTVASIKVTKDTFKPAVALQVPNSPTKASSWSKVSGVVSDKGLGAAKVRVKLIEQRSGKWYFYSGSKWEKASSERKASAQAKEITVIPTARGTWSVGIKGVKKGTLKVTYAATDKAGNTSSAKIHTQKITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC84</accession>
    <name>U5WC84_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562904.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41590"/>
    <dbReference type="EnsemblBacteria" id="AGZ46572">
        <property type="protein sequence ID" value="AGZ46572"/>
        <property type="gene ID" value="AFR_41590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8416"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040940_3_1_11"/>
    <dbReference type="OrthoDB" id="1623169at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:CoA pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="CoAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="PTHR12992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="35"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="24488" checksum="DE3873A9841166E1" modified="2014-01-22" version="1">MSERPDGLPGWWEPLLTRARSARTEDFTTLRAPASGGRAASVLVLLGEDRPGEPDLLVLQRAATMRNHAGQPAFPGGAADPEDRDAAATALREANEEVGLDPASATVLTTLPELYIPVSRFVVTPVLAWWHAPHPVHSREPAEVAHVARLPVAELVDPANRIRVRHPSGWVSPAFQVRGMLVWGFTAGVISALLDMARWARPWEPGPLVNLPDAPVTVPDTSDEVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCA2</accession>
    <name>U5WCA2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribonuclease P protein component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_42995</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42995"/>
    <dbReference type="EnsemblBacteria" id="AGZ46853">
        <property type="protein sequence ID" value="AGZ46853"/>
        <property type="gene ID" value="AFR_42995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8700"/>
    <dbReference type="eggNOG" id="COG0594">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117179_4_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000100">
        <property type="entry name" value="RNase_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020539">
        <property type="entry name" value="RNase_P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33992">
        <property type="entry name" value="PTHR33992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00825">
        <property type="entry name" value="Ribonuclease_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00188">
        <property type="entry name" value="rnpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00648">
        <property type="entry name" value="RIBONUCLEASE_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="76" mass="8224" checksum="4581518252FC0361" modified="2014-01-22" version="1">MARAGFVVSKAVGNAVVRNKVRRRLRHLVRERLADLPGGTTLVVRALPPSAAATYETLGTDLDSALAAARSSRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCK7</accession>
    <name>U5WCK7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_42240</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42240"/>
    <dbReference type="EnsemblBacteria" id="AGZ46702">
        <property type="protein sequence ID" value="AGZ46702"/>
        <property type="gene ID" value="AFR_42240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8551"/>
    <dbReference type="eggNOG" id="COG4941">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035311_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="2"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="87"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="41419" checksum="F7BD522886929FDF" modified="2014-01-22" version="1">MAVLTRVLGDLDLAEDAVQEAFVVATERWTADGVPPSPAGWIITTARNRAVDRLRRESARGAKEAQAALLFSAEPVEDPVDRLRLIFTCCHPALGQPAQVALTLRLLGGLTTPEIARAFLVPEPTIAQRIVRAKGKIRDAGIPYRVPGPAELPDRLRPVLAVVYLIFNEGYAASSGAALVRDDLCAEGLRLARMLREMMPDDPEVQGLLALLLLILSRRAARTTVDGAMVRLRDQDRSRWDAALIAEGQDLVRDCVRRGRPGPYQLQAAINAVHSAARDVSTTDWAAILHLYDQLLLVAPTPVVALNRAVAMAEVVSPGAALAVVDGLDLDRYYLFHAIRADLLRRLDRTDEARVAYETAAGLTANEAEKSFLLGAIRTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCV1</accession>
    <name>U5WCV1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_37510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45756">
        <property type="protein sequence ID" value="AGZ45756"/>
        <property type="gene ID" value="AFR_37510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37510"/>
    <dbReference type="HOGENOM" id="CLU_2033087_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="12829" checksum="54FF597D3306F6B0" modified="2014-01-22" version="1">MIKRLVIGGSITAILAAALCWWLSFLFITAFASEGLLPPDSRMPDLPPGATILDEDLSCGSGGCSRVRTVQPPPGQSPEDLADELNLSTARTENPTLLNPASVHLGAHPRGNKLIIHASYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCZ3</accession>
    <name>U5WCZ3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative membrane protein insertion efficiency factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in insertion of integral membrane proteins into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0161 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42990"/>
    <dbReference type="EnsemblBacteria" id="AGZ46852">
        <property type="protein sequence ID" value="AGZ46852"/>
        <property type="gene ID" value="AFR_42990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42990"/>
    <dbReference type="eggNOG" id="COG0759">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144811_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00386">
        <property type="entry name" value="UPF0161_YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002696">
        <property type="entry name" value="Membr_insert_effic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33383">
        <property type="entry name" value="PTHR33383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01809">
        <property type="entry name" value="YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01234">
        <property type="entry name" value="Haemolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00278">
        <property type="entry name" value="TIGR00278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="79" mass="8657" checksum="0E822EF57AD621EE" modified="2014-01-22" version="1">MLTGAVVAYRRYLSPALPARCRFYPSCSAYAQEALAKHGALRGTALAIWRLLRCHPFHPGGFDPVPDPIRHRPADVTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD48</accession>
    <name>U5WD48_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AFR_38480</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38480"/>
    <dbReference type="EnsemblBacteria" id="AGZ45950">
        <property type="protein sequence ID" value="AGZ45950"/>
        <property type="gene ID" value="AFR_38480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7785"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2887">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004900_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="12">
        <location>
            <begin position="26"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="13">
        <location>
            <begin position="535"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="349"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="505"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="935"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1109"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="401"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="424"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="960"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ45950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1473" mass="152264" checksum="365EF39F14F59998" modified="2014-01-22" version="1">MTSTPAAVPRPAYRLVRRAATPVAPLRDDPLQRHVVEHTDGPLLVVGGPGTGKTTVLVEAVAARIAEGIDPERILVLTFGRRGAGALRDRIEARISGPTFHEPLVRTFHAYAFGLLRRAAAERGEPSPRLLTGPEQDLIIRELLEVVGDPDAEDTVRWPESLRPALPTRAFAQQLRDLMQRAAERGIGPVELARLGERLGRDDWPAAARFLREYVAVLALRDATTRGSTAYDPAELVRAASGLLTDDPALLEAERRRLAYVYVDELADTDPAQIELLSLVAGGGTPLVAFGDPDSSVYGFRGADPQAVSAFPQRFRTASGALAEVITLHTSYRAEPALLTATGRLSRRMRGATRHRHLHPPATPPAPGSRPTSPGAPAGQATSGREAASAAGPPSRVVSGNKPASADQLASSGDPEASDLSGKETAASGGSAVSDFSGEETAASGGSAVSDFSGEETAASGGSAVSDVSGDQAGPLGSPGVPGVSVGGTVASGGLAASGASGDETGVLAGQAGPGSATDSSDASGRRAVAGPGDAAGQSAAEGSVADAGGAASSGGRPAGGRSGAEEGVVAGAVVRTFRSPTSETAFVAHALREAHLLHGVPWSRMAVVLRSTTLQLPSMQRALAASGVPTVTHAEDLPLHLQPAVAPFLLLLRCALEPDVLDEEASVALLHSPLGGADPLAERRLRQGLRALALAAGDRRPSGELLVDALRDPAGLDVVERRWAVPAQTVARLIATAREVAARPGATAEQVLWSVWRASGLADRWYALSTRGAPVEPGGEGGRARQWRAEAADRDLDAMVVLFDAAARFVDRLPGARTEVFLDHVLGQDLPADSIAPSADRGEAVRLLTAHAAKGLEWDVVVLAGVQEGIWPDLRLRGSLLGSERLVDFLAGRAASGAAAVVGQTSALLDEERRLFYVAATRARQRLIVTAVASASVGGSDGEEQPSRFLTELGLPDSRPGPEAPPGPGPTDPDDPGPVEPDPDPWDQPPPTPGAGAESNADAVPDAASDAAVGAAPDAASGAMASTKPGAELTDTSGAELAAVTGTNPSTVAGAELAAVPGAEPSGAPGAELAAMAGAEAEADSAELAGSQLFFDVEAADWAEVGAAEGQGHRHESSGGVTVPRQENGRVDAAPVDVAEDEQELVVGRPPRALTLSALVAELRTAVVAPDATPARRHAAAAELARLAAAGVPGAHPDEWWGLRQLSDDRPLVEEGEPVKVTPSSMESALRCSLRWLLERHGGAAPAGPAQGVGNLVHAAAMLAEDANADRERLVEYVSARFDAIELAARWLAGPEQERAQGMVDKLLRWLAKNPRRLLAIEHEFTVRLDDPARPIQLTGRVDRLEVDEQGRLVVIDLKTGKTTTVSASDVEEHAQLAGYQAAVDAGAFQEFGEESGGAALVQLGPTKEAREQMQLPLAEATDPNWAYAMVRRTADTMAAATFSAVANSKCRVCPVRTSCPISGQGRQVVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDG0</accession>
    <name>U5WDG0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_38660</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562320.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38660"/>
    <dbReference type="EnsemblBacteria" id="AGZ45986">
        <property type="protein sequence ID" value="AGZ45986"/>
        <property type="gene ID" value="AFR_38660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7822"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_587469_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="465" mass="47602" checksum="35AA4930029924BE" modified="2014-01-22" version="1">MTAVLDSRTTRRGSGAGWLLIATTSAGVVNYGYALVLTHGLAPAQYAAFAAGQALLMVRATISGAGIPWILAREIGKAPHDHERHAIVTTFAFWANLAIGVVLTALLTGGVLLFGSVQDAAVVACASLVMSVGSTGMGFLQGIGRMEMIAVLLTLEVLVKAGVGIGLVFFAHLGATGALAGFVAGSIVLLAPLPRFRRLIRPPSWRPAEATLLRAAIRQTRLQANVAVAAAGDTVLVAALGLGSTGGGPYQAASALGRVPLFASNAVSTAVFPQLSRDGSVVRKAAALRSYLLVGALMTSALVTLPDEIRSGLFPDTFATVGRWLPFAAVLGLAIGLLNLCVTFLQADDWHSGTAAYLTGIVIGYLGLIAVAGATFGVAGLAVGAAGASLLAVVALTLLSSVRGAVKLLLTSRRTLRDTTGLAALLGVLFLVENPVLWVAVAVAAGLIVLATGFPEFSVVRRRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDK2</accession>
    <name>U5WDK2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_39080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39080"/>
    <dbReference type="EnsemblBacteria" id="AGZ46070">
        <property type="protein sequence ID" value="AGZ46070"/>
        <property type="gene ID" value="AFR_39080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7909"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_2_6_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="18"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="45473" checksum="FD9EAB8610FEA44E" modified="2014-01-22" version="1">MDSDIRPPGILRGPYALTTVGAWSLVFLAAFESLAVTTIMPIVTADLDGRGLYALAFSSTLAAGVVGMVGFGSWADRRGPAVPLLVSVGTFALGLIVAGTATSMPVFVAGRFLQGLGAGGETVALYVLVAMVYPSALHIKIFGAFASAWVLPSMVGPFAAGLVADALSWHWVFLGVVALVGVSTALMLPSLKGRDRADPVRATSADARRVIEAAVVAVSVVALSSLGEIEPVIAWLLVPVAVVVIGVALRHLVPAGTYRMRAGLPATVAFCGIAGGVFFGTEVYLPLLLHDRYGLPTWLSGITLTAGAIAWALASAVQGRLGDRLDPGTAIRAGAALLAGGALTELVTVVLHLPPAVAAVGWFAAGAGMGTLYPRISTLVLALSAPGEEGFNTAAKSITDAVGASASLAVAGLLFALFPFIGPFTLTSLLGVAAVLVASGRVGTPSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDV4</accession>
    <name>U5WDV4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562425.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39185"/>
    <dbReference type="EnsemblBacteria" id="AGZ46091">
        <property type="protein sequence ID" value="AGZ46091"/>
        <property type="gene ID" value="AFR_39185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7929"/>
    <dbReference type="eggNOG" id="ENOG502ZCEC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097576_0_0_11"/>
    <dbReference type="OrthoDB" id="1490188at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="238" mass="25148" checksum="B7916B162AF92D24" modified="2014-01-22" version="1">MKVFLLLILIVVAVFAIIVFVRRGSAAREQRHLEDARADAQRWYERLGGQVLNLHGEDPAARQALSDASERYNAAGGQLQQAKSVKQFELARESALEGLTYVRAARQAMGIDPGPDLPPLASARGAGQITQEREVNVQGQHYKAGPQPGPDTPYYYPGGRVQGRPVPSGWYSTPVWKTALGAGAGVLGGMLIFDALLSPGFGDMGYGDGYQDGMQDASGDFGGGGGDFGGGDFGGGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEK4</accession>
    <name>U5WEK4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562713.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40635"/>
    <dbReference type="EnsemblBacteria" id="AGZ46381">
        <property type="protein sequence ID" value="AGZ46381"/>
        <property type="gene ID" value="AFR_40635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8224"/>
    <dbReference type="eggNOG" id="COG2110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046550_5_1_11"/>
    <dbReference type="OrthoDB" id="1812319at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="178" mass="18715" checksum="F541164ABA04AFC1" modified="2014-01-22" version="1">MRIDLIEGDITTHRTDAIVNAANSSLLGGGGVDGAIHRKGGPEILAETRALRASQYGRGLKTGQAVATTAGRLPARWVIHTVGPVFSTTEDRSDLLRSCYENSLRIADELGAESIAFPLISAGIYRWPLEDAVKQALGVLRAAEPANLRLATLVLFGAETAATARRVSAELGLPAGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEL3</accession>
    <name>U5WEL3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RDD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_41155</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41155"/>
    <dbReference type="EnsemblBacteria" id="AGZ46485">
        <property type="protein sequence ID" value="AGZ46485"/>
        <property type="gene ID" value="AFR_41155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8330"/>
    <dbReference type="eggNOG" id="COG1714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079635_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="7">
        <location>
            <begin position="56"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="23629" checksum="1D1D064566283535" modified="2014-01-22" version="1">MPGGPMPGGPVPGGPAPAFGGPMPGGPAPAYYGPPPPGYGWRPPPVPVAPDGRPLADFGIRLLSHLIDGAIVGGIATVVFLPVMFIFVFNVMPDFPSAADPSYTPTFDPAEFFIPFLLLELGFFVLLFVAYYVYYVEMMYRSGQTVGKKLMKIKVVPLEPGAVLTRGIAAKRYLIEFVVGIIVPFFTYLDGLWQLWDKPYQQTLHDKVAKTVVVKVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFI8</accession>
    <name>U5WFI8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MDMPI_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563136.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42755"/>
    <dbReference type="EnsemblBacteria" id="AGZ46805">
        <property type="protein sequence ID" value="AGZ46805"/>
        <property type="gene ID" value="AFR_42755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8653"/>
    <dbReference type="eggNOG" id="ENOG50331BS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106669_0_0_11"/>
    <dbReference type="OrthoDB" id="1760896at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024344">
        <property type="entry name" value="MDMPI_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11716">
        <property type="entry name" value="MDMPI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MDMPI_N" evidence="1">
        <location>
            <begin position="10"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="23557" checksum="366C9A9904457528" modified="2014-01-22" version="1">MRLPDVATALEAAYTGLTDVVADLDDLELLLPSGCRGWTICDLLLHVSLDAQRALVAFAVPVDGPADVDFVSYWRSFAGVGDASAASAHAQWVRRTAAAFNQPSGVVQRWTETSAAAVSVAHRADPGSFIGTQEHVLAVPDFTATLVTEAVIHHLDLIVSLPDAPEPPPEAIEIALSTLDGLAAPVGLPAHWSEHETLLKGTGREELTAADRSAVEGLTFPLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFK4</accession>
    <name>U5WFK4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DGPFAETKE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563032.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42235"/>
    <dbReference type="EnsemblBacteria" id="AGZ46701">
        <property type="protein sequence ID" value="AGZ46701"/>
        <property type="gene ID" value="AFR_42235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8550"/>
    <dbReference type="eggNOG" id="COG3795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130902_2_2_11"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="5"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="113" mass="12148" checksum="1F5A7DB8542C7011" modified="2014-01-22" version="1">MKTYLISIFQPDGPPPPPEQLEPIMRDLDALNQELEDTGAFVFSAGLDTPGTARVVRASGDGFLLTDGPFLEGKEHLGGFTVITAPDLATALKWGERMAAVTSLPIEVRPFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VN06</accession>
    <name>U5VN06_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00370"/>
    <dbReference type="EnsemblBacteria" id="AGZ38363">
        <property type="protein sequence ID" value="AGZ38363"/>
        <property type="gene ID" value="AFR_00370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.76"/>
    <dbReference type="eggNOG" id="COG4683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164818_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009241">
        <property type="entry name" value="HigB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05973">
        <property type="entry name" value="Gp49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="10804" checksum="A2BF64D07DE6D2A1" modified="2014-01-22" version="1">MADILAERAETLGEPYSRHLSGKTRELRFHLDRSAVRISYWLASGARAVLLTVFRKTRQIERAEVARAVAAQQVCESQHEAHAPATEIYDRKWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNB0</accession>
    <name>U5VNB0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357401.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00845"/>
    <dbReference type="EnsemblBacteria" id="AGZ38458">
        <property type="protein sequence ID" value="AGZ38458"/>
        <property type="gene ID" value="AFR_00845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.169"/>
    <dbReference type="eggNOG" id="ENOG5034BK2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1479075_0_0_11"/>
    <dbReference type="OrthoDB" id="2148562at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="19400" checksum="D764D26D4363157D" modified="2014-01-22" version="1">MTVIDAPATGRSTSPRWPALAAAWYIGIFCLVTSNVVLWTFFQGKTLTDNGVVVLAIVLRLVTVLLAVASIAGWGRFVPGWLLLAGLWGAAAVQLAYPIAETVVKALILTGVMEPINKGISNMSAEGWFNFGATWLIWGVPGLLFVFAARAYARRVHVDKRWVVLGILGGLGLLLGLGVIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPD5</accession>
    <name>U5VPD5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02720"/>
    <dbReference type="EnsemblBacteria" id="AGZ38833">
        <property type="protein sequence ID" value="AGZ38833"/>
        <property type="gene ID" value="AFR_02720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.547"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="36198" checksum="3C9B622D3278DABF" modified="2014-01-22" version="1">MEGTVLSVVVPMYNEEAVIPALVERLRPVLDNLGVTYEVVAVDDGSRDRTAHLLFEYGRTWPELRLVKLRRNSGHQAALTAGLQRAKGDWIVSIDADLQDPPETIAEMLRVAREQGLDVVYGVRSDRSTDTVFKRHTAGAYYRLMRRMVGGDVPAQAGDFRLLSRDVVEVLKKLPERAPVYRLLVPSLGFASAEVTYARERRAAGETKYPLRKMVALAWDSTANFSAAPLRIATWLGAFSFVACIALMIFGVVVWANGTVIPGWTSLFLAVLLLSAVQLICLGLLGEYVARIYRTVQDRPTFHIGFDSAAEAQRIEIPQQRSAAALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPY2</accession>
    <name>U5VPY2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357958.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03620"/>
    <dbReference type="EnsemblBacteria" id="AGZ39013">
        <property type="protein sequence ID" value="AGZ39013"/>
        <property type="gene ID" value="AFR_03620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.729"/>
    <dbReference type="eggNOG" id="COG4886">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054192_0_0_11"/>
    <dbReference type="OrthoDB" id="1499307at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="34540" checksum="E1DA1426D6B09261" modified="2014-01-22" version="1">MSFYEHATEFAGRRVVDVPLDGPLPPVDGPVAWRFSFWEFDETTTRGLNEKFREAFEAFVERSGGEIEALVIGAWGYAAFDEAPIAQLCEAASRLPRLRALFLGDQTSEECEVSWMRVGDVTPLLAAYPELEILKVRGGSELLTFDQVRHESLRELVFESGGLPSGVVREVLASEFPALTSLELWLGVDEYGGDTHVELLAPLLAGELFPALRHLGLRNSEIVDDIAVALAAAPIVPRLEVLDLSLGTMTDAGAGALLAGQSLSHLRRLDLHHHYLSPEMSARVVAALPGVLVDVSDVQEADEDDDEVYRYTAVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQD0</accession>
    <name>U5VQD0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358078.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04220"/>
    <dbReference type="EnsemblBacteria" id="AGZ39133">
        <property type="protein sequence ID" value="AGZ39133"/>
        <property type="gene ID" value="AFR_04220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.852"/>
    <dbReference type="HOGENOM" id="CLU_862307_0_0_11"/>
    <dbReference type="OrthoDB" id="2161590at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="34561" checksum="D72D76825B126C27" modified="2014-01-22" version="1">MPDQLEKLFTDLRAGVLPEVRPPGTAAARHTVRRRRTTRTVVAAATAALAVTGGVVAINLPRTPERQESPADLTGSARLALDSQLPKLTGRLRTGAVEAEGRLTFPDLAAGGYTLAVTCAGAGSVRVDAELVRSAEETAVLGGQIVSCAHDALVSVMTFRLPDRGPVVITAEGDGSAAGHSAYALELGDGDGSGDYRPEGALYDPDAVPSDESSWNAGRAAAVFPQGGAGVPVQVTTEQLRGEEATGYSRTGDFELQVVCSGPGNLTMTVGAVPPGGDLTADSQALVTAEVPCNVDPATSLNDILSLPKGTTLLIVTVPDQAAWNRAGWAYRVTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQE9</accession>
    <name>U5VQE9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_04345</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358110.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04345"/>
    <dbReference type="EnsemblBacteria" id="AGZ39158">
        <property type="protein sequence ID" value="AGZ39158"/>
        <property type="gene ID" value="AFR_04345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.877"/>
    <dbReference type="eggNOG" id="COG3795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130902_2_2_11"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="3">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="116" mass="12737" checksum="0D7C332211A76A53" modified="2014-01-22" version="1">MRYVMILTGDESNWFDPEQAESLMDEVGTWWEKWHQEGKIVKGGAELQPSSTAKTVGPGADGKPTVTDGPYLEIKEVVGGFIHVVADNIDEAVAVASGWPGIRLGDRVEVRPIMER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQJ4</accession>
    <name>U5VQJ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_04670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39223">
        <property type="protein sequence ID" value="AGZ39223"/>
        <property type="gene ID" value="AFR_04670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.943"/>
    <dbReference type="eggNOG" id="ENOG5033WXY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090251_0_0_11"/>
    <dbReference type="OrthoDB" id="1945039at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="22765" checksum="07E2863A3EA9E4F7" modified="2014-01-22" version="1">MAALIITSIVGLSAFFIIADERRGHGGDSAAPPPPVSSLAISSRALDDKPLTQGEVFPEQEIRLVSGAAPYQVTMTHIDTDCQIATTGDLGALLAENDCSQVVRAGLTAPFGGYQVTAGIFNLADEGSAALVTDKAGDLVEEGRGSFAAMGGLGSDPLAEPLAQVGWHSRGHFLLYCVIARPDGQLVADDDPYAERITADLVEQYLGETIVAKRAIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR75</accession>
    <name>U5VR75_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional purine biosynthesis protein PurH</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">Phosphoribosylaminoimidazolecarboxamide formyltransferase</fullName>
                <ecNumber evidence="8">2.1.2.3</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">AICAR transformylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="8">IMP cyclohydrolase</fullName>
                <ecNumber evidence="8">3.5.4.10</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">ATIC</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="8">IMP synthase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="8">Inosinicase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">purH</name>
        <name evidence="10" type="ORF">AFR_05115</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The IMP cyclohydrolase activity resides in the N-terminal region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PurH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.3" evidence="8"/>
    <dbReference type="EC" id="3.5.4.10" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05115"/>
    <dbReference type="EnsemblBacteria" id="AGZ39312">
        <property type="protein sequence ID" value="AGZ39312"/>
        <property type="gene ID" value="AFR_05115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1035"/>
    <dbReference type="eggNOG" id="COG0138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016316_5_2_11"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="PTHR11692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="503" mass="52959" checksum="DE2CD04036A5FF81" modified="2014-01-22" version="1">MYDKTGLVELAQALHAAGVQVVSTGSTASTVEAAGVPVTRVEELTGFPETLDGRVKTLHPKVHAGLLADLRLPAHEAQLAELQIEPFDLLVSNLYPFTETVASGATVDECVEQIDIGGPAMVRAAAKNHPSVAVVTSVAAYPLILDAVRDGGFTLTQRKLLAARAFADIAEYDVAVANWCATTLAPSEWPEFAGLALKRESALRYGENPHQQAALYLDPNAPAGLAQAVQLHGKEMSYNNYVDADAAWRSANDFTEPCVAIIKHANPCGIAVGADVAEAHRKAHACDPVSAFGGVIAVNRQVTVELADQLDGIFTEVVVAPSFTEEALAVFREKKNLRVLVAPAWNPPPAEIKQVGGGVLVQMSDRIDAEGDNPANWTLATGEAASPEDLADLAFAWRAIRSVKSNAILLAHDGATVGVGMGQVNRVDSAHLAVNRAGADRAKGSVAASDAFFPFPDGLEVLIAAGIKAVVQPGGSIRDNLVIEAAEKAGLTVYLTGTRHFYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS70</accession>
    <name>U5VS70_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359183.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06815"/>
    <dbReference type="EnsemblBacteria" id="AGZ39652">
        <property type="protein sequence ID" value="AGZ39652"/>
        <property type="gene ID" value="AFR_06815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1392"/>
    <dbReference type="eggNOG" id="COG4961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257653_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="103" mass="10544" checksum="B1F2E0572B7D2842" modified="2014-01-22" version="1">MIDFGMLLNRQLLLTEAAREGARVAALTGSASQASDRATTFLGDSGLGSTTVTAVTVCDATSTPSSAPAEVDLSYAYTPLTPLGDLMNYSFTLTAKGVMSCVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSI5</accession>
    <name>U5VSI5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-galactoside-specific lectin 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359360.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07700"/>
    <dbReference type="EnsemblBacteria" id="AGZ39829">
        <property type="protein sequence ID" value="AGZ39829"/>
        <property type="gene ID" value="AFR_07700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1570"/>
    <dbReference type="eggNOG" id="COG4726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_540411_0_0_11"/>
    <dbReference type="OrthoDB" id="1307506at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ricin B-type lectin" evidence="1">
        <location>
            <begin position="171"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="1">
        <location>
            <begin position="350"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="517" mass="54472" checksum="277BFA3AF700CB63" modified="2014-01-22" version="1">MRTRWWRRPSDDRGSLPMAILLITIGMGLSALLATTVNSQIAATRSTIHSSEALDAAQSGLDSALGRIRLATAANGTGDFRKLPCGPFSGTVNGGTDQTYSVGVYYLATEPPAGDVTWAQANKLPCTSTYLAAGSPVYVLLSSTGKALNSGSPRTVTATYTLHSKTRENIAGGLIHLYGPHNPDLCFAAPGTRPVAGDPVTMQVCDSTSDAQLFAYESSLNLVLVSSRADGTTGMCLDAAPVNLEQVVFKACSATPIARQQWSLNDRANFEGTTNGTDLNAQCFHLATPGTVGSTIVLHAEASDPAGVSQYDRACYSDYTDSRSFAPDAAAGTGRAGPGTNQLVNFQQFGRCLDVAGDNVAAGFLVIWPCKQKPSGAIQWNQVWHLPDIATGATSATGRIWLHNDRDNQDYCLTSPGSTAARQYVSVQLCPATIGASMTWTRYAATGVFGTAYRIESAFQAPAGTTWCLTGTDSTDFWAGHGDVSKAVMAPCSGSTLQKWNASPMVLQSAMTNVSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSV8</accession>
    <name>U5VSV8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357753.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02605"/>
    <dbReference type="EnsemblBacteria" id="AGZ38810">
        <property type="protein sequence ID" value="AGZ38810"/>
        <property type="gene ID" value="AFR_02605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.524"/>
    <dbReference type="eggNOG" id="ENOG5031V5W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_779936_0_0_11"/>
    <dbReference type="OrthoDB" id="2176330at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="355" mass="36512" checksum="531658CC2C48597D" modified="2014-01-22" version="1">MVSAPSTPTTRRPGAHHKPPTTRSAPEWVRRLALGGAIALPYVLLAIFRGVGPDTPNAKLDRLGSEIKWGSSDLGFVRDMYPPLPSGIAGALPGGRLALGIAGALLAAFVLEFLITRLRRRQHPWWLIAPVIIGIGAMPVYARTAIDDLATFAGLAALIFAVAGLLEFTGLGDTGGGFRAGLALGIGVACDLNVAVYAVAIGVAAPLIARRRYQGIPYATQAAMLVLIFPACAALTGWAYLEWRFSGGAFHAVHLVGGGTDGLTATLLAIATSPVFLLSAVILLRQNAAAAVALAIPVLSLMISTGLGLTNGGGTDHIVLALIGAFTIAANPSLTVIRLYALAVVIQVGLGSVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSY0</accession>
    <name>U5VSY0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">dGTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359453.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08165"/>
    <dbReference type="EnsemblBacteria" id="AGZ39922">
        <property type="protein sequence ID" value="AGZ39922"/>
        <property type="gene ID" value="AFR_08165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1666"/>
    <dbReference type="eggNOG" id="COG0232">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028163_0_1_11"/>
    <dbReference type="OrthoDB" id="370035at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006261">
        <property type="entry name" value="dNTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020779">
        <property type="entry name" value="dNTPase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026875">
        <property type="entry name" value="PHydrolase_assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF32">
        <property type="entry name" value="PTHR11373:SF32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13286">
        <property type="entry name" value="HD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01353">
        <property type="entry name" value="dGTP_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="58"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="406" mass="44595" checksum="0B0748B00AE6AFA4" modified="2014-01-22" version="1">MVDGDASRWVREPVKDSGYGRTQFERDRARVLHSAGFRRLAAKTQVHTAGSDDFLRTRLTHSLEVAQISREMGARLGCDPDVVDVAGLAHDIGHPPFGHNGEAALHLAAEACGGFEGNAQTLRVLTRLEAKVDGAGLNLTRASLDASCKYPWPRRDGVRKFGVYDDDRPVFDWIRATAPDGERQCLEAQVMDWADDVAYSVHDVEDGIHGGYLSLKPLLLDAGERAELCTDVANTYSSETPEALGEALRLLLADDVVIATADYDGGHRSQVALKRMTSVLTGRFVSSAVGATNSRHGSEPVRRYDVDLIVPRTVRNQCALLKGMALRYVMRARAAEQWYEQQRAILTDLVSVLSERAPDHLDPMFAEMWKAAGTDTARLRVVIDQVASLTDPAAVAWHRTLMAADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTL3</accession>
    <name>U5VTL3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_09215</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359710.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09215"/>
    <dbReference type="EnsemblBacteria" id="AGZ40132">
        <property type="protein sequence ID" value="AGZ40132"/>
        <property type="gene ID" value="AFR_09215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1876"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_40_3_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="12"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="21223" checksum="00D176396920B5AD" modified="2014-01-22" version="1">MSVQERKERERADRERLILATAREIAEDQGWDAVTTRRLAERIEYSQPVLYSHFRGKREIIGGVALQGAAEMAVALRAAASAADGPRARVTALARTYLDFAARSPAVYDAMFQLDGGLAFAQEDTPAPLQDAFAALLESLGEVAGNGVDPGLFTEVFWASLHGLATLTRAGRLPPAETERRTELLVDRLAVLSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTZ3</accession>
    <name>U5VTZ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360370.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10895"/>
    <dbReference type="EnsemblBacteria" id="AGZ40468">
        <property type="protein sequence ID" value="AGZ40468"/>
        <property type="gene ID" value="AFR_10895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2219"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_15_12_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="21411" checksum="3FB7680D7E5C02F4" modified="2014-01-22" version="1">MPRVSQDQLDARRHEILAAARGCFARFGYEGATVRRLEDATGLSRGAIFHHFRDKDSLFLAVAEDDAAAMVETVARDGLVQVMRDLLDQARSSDGDTAGWLGTQLEVSRRLRTDPEFAKRWTERAAAIADATRERLERQREAGVLRDDVPVEVLTQFLELAYDGLVLHLATGRPAGDLSRVLDVVEEAVRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU13</accession>
    <name>U5VU13_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative TraR/DksA-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_10065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360046.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10065"/>
    <dbReference type="EnsemblBacteria" id="AGZ40302">
        <property type="protein sequence ID" value="AGZ40302"/>
        <property type="gene ID" value="AFR_10065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2051"/>
    <dbReference type="eggNOG" id="COG1734">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043144_3_0_11"/>
    <dbReference type="OrthoDB" id="1932026at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037187">
        <property type="entry name" value="DnaK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020460">
        <property type="entry name" value="Znf_C4-type_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020458">
        <property type="entry name" value="Znf_DskA_TraR_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01258">
        <property type="entry name" value="zf-dskA_traR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00618">
        <property type="entry name" value="DKSAZNFINGER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109635">
        <property type="entry name" value="SSF109635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01102">
        <property type="entry name" value="ZF_DKSA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51128">
        <property type="entry name" value="ZF_DKSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DksA C4-type" evidence="6">
        <location>
            <begin position="66"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="dksA C4-type" evidence="4">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15746" checksum="AA861B1B2B67EB8C" modified="2014-01-22" version="1">MAKATETRSASAAKTAPRKARSAEETETIRVALEERREELQAEYDQSLTEITELQRERLADSAGDDQADTGTKTFEREQEITLANNLLERVTQVERAIERLGEGNYGWCERCGNAIPVERLAAFPVATLCVSCKQLEERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU14</accession>
    <name>U5VU14_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">bPH_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360407.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10995"/>
    <dbReference type="EnsemblBacteria" id="AGZ40488">
        <property type="protein sequence ID" value="AGZ40488"/>
        <property type="gene ID" value="AFR_10995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2239"/>
    <dbReference type="eggNOG" id="COG3402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104197_0_0_11"/>
    <dbReference type="OrthoDB" id="1768296at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="DUF304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_2" evidence="2">
        <location>
            <begin position="85"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="18964" checksum="8F6E337BE57C6775" modified="2014-01-22" version="1">MTESGAPAPAPTDPLEPWPDTVTWRPVSRSLITVELINRAVWVLILLAGLTTAWTITDHPAWIAAMAAVVIFAVWRSIITVRAVKAWGYAERDNDLLVRHGLVIRRLSIVPYARMQFVDVTAGPLERAFKLATVQLHTASAASDAKIPGLPPEEASRLRDRLTALGEDRAEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU30</accession>
    <name>U5VU30_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_11070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360436.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11070"/>
    <dbReference type="EnsemblBacteria" id="AGZ40503">
        <property type="protein sequence ID" value="AGZ40503"/>
        <property type="gene ID" value="AFR_11070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2255"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_7_0_11"/>
    <dbReference type="OrthoDB" id="1308416at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="23"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="164" mass="18073" checksum="B49A4C3EA9F7B9BA" modified="2014-01-22" version="1">MASSWEESYIGGLRTLAGDDRVLISIGAHSLVRDPQGRIALIQRSDDRTWALPGGTIELGETLRDCAVREVREECGLIAHSVTPFGLYTRAKDWSPNVFGHRYQHIALLCRVDSYEGEILRETDESVDAGWFSPDALPEGTGGLVLRALEDLAAFERNGVFALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU42</accession>
    <name>U5VU42_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH-quinone oxidoreductase subunit K</fullName>
            <ecNumber evidence="5">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NADH dehydrogenase I subunit K</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NDH-1 subunit K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nuoK</name>
        <name evidence="6" type="ORF">AFR_04260</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the complex I subunit 4L family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358086.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04260"/>
    <dbReference type="EnsemblBacteria" id="AGZ39141">
        <property type="protein sequence ID" value="AGZ39141"/>
        <property type="gene ID" value="AFR_04260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.860"/>
    <dbReference type="eggNOG" id="COG0713">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144724_1_0_11"/>
    <dbReference type="OrthoDB" id="2081663at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01456">
        <property type="entry name" value="NDH1_NuoK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001133">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain4L/K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434">
        <property type="entry name" value="PTHR11434"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0874">Quinone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13531" checksum="7805F6A4604D024A" modified="2014-01-22" version="1">MHAVIPYVTAALLFGLGVYGVLRRRNAILVLMAVELMLNAINLIFITADASVRAALVGVQIEAWAYPNGEPPGYSGGVFALFIIVLAAAEVGVGLAIVLQYYRMRQAVHLDEVELHHESDGQVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU61</accession>
    <name>U5VU61_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ECF subfamily RNA polymerase sigma-24 factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_10600</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360257.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10600"/>
    <dbReference type="EnsemblBacteria" id="AGZ40409">
        <property type="protein sequence ID" value="AGZ40409"/>
        <property type="gene ID" value="AFR_10600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2161"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_3_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="42"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="134"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="21414" checksum="7557F8D5F39EA298" modified="2014-01-22" version="1">MTFVLPLPRPLLRLLRSVVSGSSPVVRRAAGEGPPAINELYHARRLSLVRLAVLMVDDLPTAEDIVQDVFAALYRRHGADLRGVADPHAYLTTGVMNAARSALRRRRSARAYVPPRPGLAPAAEDEVLLGQGDREVLAALGELTVRQRQVMVLRYWSELSEQEIAETLHVSRGTVKSTASRALTVLRRLLGEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUP0</accession>
    <name>U5VUP0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Excisionase family DNA binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05805"/>
    <dbReference type="EnsemblBacteria" id="AGZ39450">
        <property type="protein sequence ID" value="AGZ39450"/>
        <property type="gene ID" value="AFR_05805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1179"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140176_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="4"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="70" mass="8000" checksum="5FC1B055100CBA0D" modified="2014-01-22" version="1">MLLSDVAAELNVSDSQVYHLVRSGDLPAIKIGGRGQWRVERAKLEEYIQQKYDETAVWVENNPLTDRDPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUS8</accession>
    <name>U5VUS8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Abortive infection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360807.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12310"/>
    <dbReference type="EnsemblBacteria" id="AGZ40748">
        <property type="protein sequence ID" value="AGZ40748"/>
        <property type="gene ID" value="AFR_12310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2499"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052492_1_0_11"/>
    <dbReference type="OrthoDB" id="1623593at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="30967" checksum="EBA71D24943B8C09" modified="2014-01-22" version="1">MSNVEYHRVYAGERRRVGRGLLAIALLLGGLLVVSVVLSVVATVLDDRGADGAYTPMMHAAAMLSLALLIPWSMAIQRWLYGLPGSSLHSVFSRFRFALFGRALLLIGPALVAALVVSDYLAPEPRNVWSDTDVLWMVAATLLLTPLQAAGEEYGFRGLIFRAAGGWTRGARSGLVVGLITMTVAFAASHAAGDPWLNLWYVVLAVGTGLITWRTGGLEVAIVLHALWNTLGFVFSIAMRSDLNAVVTDRDAGAATAATLIPCAVVVVTVAIVWYTTPRIGPLAAEAGSNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUT3</accession>
    <name>U5VUT3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Roadblock/LC7 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360508.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11265"/>
    <dbReference type="EnsemblBacteria" id="AGZ40542">
        <property type="protein sequence ID" value="AGZ40542"/>
        <property type="gene ID" value="AFR_11265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2295"/>
    <dbReference type="eggNOG" id="COG2018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094585_2_0_11"/>
    <dbReference type="OrthoDB" id="1723871at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="1">
        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="141" mass="14447" checksum="B97D13B4A1E3A3AB" modified="2014-01-22" version="1">MQSNDPVRPAQDLVWLLTGLIERVPDTRSALLLSSDGLRKAAYGLDDAAADHLAAISSGLFSLARSAGTRFGGGETVRQVVAELQDALLFVSAAGSGAVLAVMAGPQAEPGVLGYEMAQLVKSVRPYLGTPVRSAAGGAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV25</accession>
    <name>U5VV25_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alpha-N-arabinofuranosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_12835</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360912.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12835"/>
    <dbReference type="EnsemblBacteria" id="AGZ40853">
        <property type="protein sequence ID" value="AGZ40853"/>
        <property type="gene ID" value="AFR_12835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2607"/>
    <dbReference type="eggNOG" id="COG3940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009397_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665248" evidence="5">
        <location>
            <begin position="25"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="6">
        <location>
            <begin position="341"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="2">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="469" mass="49906" checksum="44C4F6F8578D48EF" modified="2014-01-22" version="1">MAVVAVLLAGLALVVADAPQPARAAGTFVNPVSPRPDPFVVFHRGRYYTVENDTSETRLLIRSAATVAGLSAATPQLVWADGNTSRNKQVWAPSLLNLNDRWYLYYTASDGVDNNHRNYVLESTGLVSQGATPAGPYAFKARIFDPAADSWAIDGLPFTHNGALYYAYAGALGNQHNEMFIARMANPYSLSGARTHLPLAGGCPTIREAPATLNRNGRTWLVYSTCDTGTPDYQLWMTSIADGADPLVATNWRQRSGAVYQRNDAAGVFAPGSNNFFRSPDGTEDWMAYHAKTTSAFTYDGRTTRLQKIGWNTDGSPDLGRPIALGTAQATPSGDPGPSAPAAGTRIAGPGGKCVDVAGDDTGANLSPVQLWDCLGAAADQLWTWNGTSLRTLGRCLDVAGNITAAGTRLQLWDCNGAGGQQWVQQANGSMLNPQSGRCLDSPSGATANGTRLQIWDCNGSPAQVFRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV32</accession>
    <name>U5VV32_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360702.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11815"/>
    <dbReference type="EnsemblBacteria" id="AGZ40652">
        <property type="protein sequence ID" value="AGZ40652"/>
        <property type="gene ID" value="AFR_11815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2406"/>
    <dbReference type="eggNOG" id="ENOG5032SNS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106645_3_0_11"/>
    <dbReference type="OrthoDB" id="1719670at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="15062" checksum="40219BB04C0E32B0" modified="2014-01-22" version="1">MATVCVEATINAAAQQVWEAVADVGAVHQRLLPGRVADARMEGDVRILTMPDGAQVREVILAIDHSIRRMAYTVTEGQRLPLTYHHASFQVFEEGDRSRLVWLTDVLPHALAPVVQGRVERGIQEMKAILEAAAVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV41</accession>
    <name>U5VV41_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2017 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359086.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06330"/>
    <dbReference type="EnsemblBacteria" id="AGZ39555">
        <property type="protein sequence ID" value="AGZ39555"/>
        <property type="gene ID" value="AFR_06330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1284"/>
    <dbReference type="eggNOG" id="ENOG5032Z6K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087287_3_0_11"/>
    <dbReference type="OrthoDB" id="1738659at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018561">
        <property type="entry name" value="DUF2017"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09438">
        <property type="entry name" value="DUF2017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2017" evidence="1">
        <location>
            <begin position="39"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="18500" checksum="F423E220CB4D668E" modified="2014-01-22" version="1">MFRRNGSQCVATFAVDEVRVLRKVAGEVVGLLMDGFDHTDPVVGRLFPDIYPDRPDDSAEFRLYTEGDLKTGKIDQAGAILAALPDDGPGEVRLDGEEAEAWLRAINDARLAMGTRLDIQGDTDLGDELDDAVIRDPASSRVFQLSVYAYLGYLQESLLNALPEIQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV68</accession>
    <name>U5VV68_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06505</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06505"/>
    <dbReference type="EnsemblBacteria" id="AGZ39590">
        <property type="protein sequence ID" value="AGZ39590"/>
        <property type="gene ID" value="AFR_06505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1319"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_247424_0_0_11"/>
    <dbReference type="OrthoDB" id="1691666at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666113" evidence="2">
        <location>
            <begin position="28"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="882"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="959"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="615"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="816"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1091"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1173"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1212"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1324"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1354"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1372"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1389"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1450"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1510"/>
            <end position="1527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1527" mass="149798" checksum="DF6B5FAC29EE921E" modified="2014-01-22" version="1">MRVFRTILGMLLLTVGLPTLLAGGAFWAAMQHRDPGGAFTGDMQRVATSGYAVVVDDFDGLLKNDAPFVRFGDTRMRLTAQSGGAPAFIGIAPASDVSRYLTDVPQIGVSSIDLGTGALPVTSKLRPGTIAPEQLPGRQRFWLKKGAGTLDWNPAELRDTRYSLVIMNPAAQPGVRLESTAAVLPGWLNQATWALLILGSLLVMVGMIVLGWPSRRREVVYIVEPSQVPDLMQAIGAPLPLSRTGGGRHAGTHRPRTLADSQPRSRPPALTWPPSSSSPSSGRGSAIPMPAAPVSGAATSVTEPAMATASAASGASAAPTPVAAGSVATASSTAASSTAASSTAAPDSAASGSPAPGAVTSGSAVSGSATSGSAASGSVAPAQAKPAPGEPLNLISTSPAPGSVSDPSPITSPADPLFSRSSTAAPDRANRRRNAAQPTDLPIFEASAVGAWVAETAAARARETEARAAAALNASRKSTAAASVPSGYDVVVVGLTESATDSAASPAAGKAAQTRDLSAAAAGKAAANQDLSAAAAGKAAASQDLSAATGRADQTTAATKPEEPNAIATVATRLQPATPAGEENTTSSTESASAAGKVVPSSVTPTAPKAADESRPAAPKPPVPAPPKHPVPTPPKPPVPAPPKPAGDTDSRSTVPSTSATAGEVRSATDSVVPAMKAGEARPAAAEPVASAMKAGDAASAASDSAVSAVRAGEVRSAAPDSAVPAVKAGEARSAASDSAVSAKSAVEAGSAASESAAAAAKAGDARSAVPGSAVSAASAAKAGEGQSVAPGSVVSGVPAGSKAVTPPKAEVAGPTGSNSADQAAGQVSAPNVTKPAAAPSISSAAAKGTDSASADDLTLPASREAGAAGGNASRIAGPASAAAKAAAPQVAREGVSAAPRIADEAGEGGKAAPQVAAESWLIASAGAAEGVPITPRGAAEEAAARAAAAGAAATRAAANAAAARASAAEAVEASEAAKSKAATSGGDGTAAPAKAARPAGATPRNALFGGSATPGWAAASGLTRADSGRVGVAKTPAADKPEAASPAAKGRPSAVPAEKPVPKPNPTSPNRPVPTTPARPAASTKPPAGSDSPAGESTTAAPSPGNRPGSSAPAQSTGPARSGGQSSPITSHHITQSVPGDGGTARPTGPSSGSKTPTSAGTDPAGPNPAQAATAGQGTASGPTTGGSSTPGSAEKRVQTPAAQNTAPTPTRPAPTDPVPVPPNSPAQPRPSVPGPSRPSDPDREPVPAAHGTGGGVRSASPVTDPAADPQRNNPVWPINSAGVPNAAAQADAGQRITEQRIAGLRPAVEQPRTEQLKSEQARNEQLRSGQAGNEQLRSDQARNEHLKSQQARNEQPRTGQAGSEAQRLETQKAEAQKVESPKTEPLKAEAQATTVQATTAQATTAQTAEAQTTTAQQAEAPKTGQQAAAGLPKAEPDKSGDATATNPEAPAKEDKKRKAESSLSRLAGYKAEVAELLGGDSAARRRRKNTTASGLPAPDPASQEPPIKLTRPDRKGGRRQQPPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVF6</accession>
    <name>U5VVF6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage II sporulation protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07005"/>
    <dbReference type="EnsemblBacteria" id="AGZ39690">
        <property type="protein sequence ID" value="AGZ39690"/>
        <property type="gene ID" value="AFR_07005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1429"/>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043255_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="158"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="377" mass="40762" checksum="05C8741882C3E122" modified="2014-01-22" version="1">MSDRLIAVQRALAGAPADRIVECLTEAIVQEYALAGAELLLVDYRLAALLPLLDGPAVTQPGDPAWRCFDHQTEIAAGHLTYLPVTGRGERMGVLRLSPTPASDEARAELAEIATRLAHELQAASAGTDRYAVAARTTRLTLAAEMQWELLPGRSRVRPSFSLAGQLEPSYAVRGDSFDWAEQNGCLYLTVLNGHGEGMSAALLTSLATFALRNARRAGLELADQAALADQAVFAQHRGDTHVETLLLELNLDTGELLAVDAGSPRMMVLRDGEVLDRPLEAQFPLGMFETSEYTPQSFTLQAGDRLFIVSDGVFDAATETSRYGEAALHRFMRRTGSLAPLQAVRSLLGDLRAFVRGDLEDDAVAVCLDWTGKSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVK1</accession>
    <name>U5VVK1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361087.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41028">
        <property type="protein sequence ID" value="AGZ41028"/>
        <property type="gene ID" value="AFR_13710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2784"/>
    <dbReference type="eggNOG" id="COG3371">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080422_1_0_11"/>
    <dbReference type="OrthoDB" id="1497902at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="24581" checksum="78E61492A0E354E1" modified="2014-01-22" version="1">MMEMDTAKSTPSRIVGPALIAAVLVNLIAEAVVAAAWDRRPYSYVEDYVNFLGNPFVGDFEGFLISSPLWWLMSIAWIVSGTLVAAASVALSRRLTGWRKRTIAGLGLAQGFALILFAAFPLGPARFDDGTLVLYLIGAFLSIIAGNVLAIVTGLSGAALGLPRWLTRTSIALGAIGLLNIPLTYGWTPTGIAERISLYSFLLWALITGLVLLRSSTGKARMPVTAPRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVM6</accession>
    <name>U5VVM6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13190"/>
    <dbReference type="EnsemblBacteria" id="AGZ40924">
        <property type="protein sequence ID" value="AGZ40924"/>
        <property type="gene ID" value="AFR_13190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2678"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_12_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665387" evidence="1">
        <location>
            <begin position="46"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="456" mass="48441" checksum="2D6F0B91C5447E24" modified="2014-01-22" version="1">MTRVSTGRRAAGRTRPLYAILALSGTLVAAACSAPGGSSTTTANSAPASVTTTCGTDPVTLKGYFETGFPLPKTLADEFTAQFPNVKFDIREDQFAVITQNAPRVLSDDPPDLMRLPQVSELAKDNLLLNMDTYAKAYGWDKWPPSQLEQMRFGSNGERGDGSLVAMGLNFSMTGIFYNKKLAAQIGMTEAPKTLAEFDTVLQKAKDAGLTPINQFNGGATGGLAFPLQNLMAAYGEPGPINDWIFNKQGATIDTPSNLQATQHLEKWIKSGYLAKDINSQDYATMMSRFIGGKSLFIFSGDWESGNLDKQMAGNAGFFLMPPAQAGGKQAAMSAPLTYGISAKSKNADCAAFFLNWVATDQKARDIGVKVGGSHPMGPDNAYMPEVAQGTVTASTLAAGATIGKDNGAMDFIANATGAIYAKSWTPQLQKLVAGQQTPQGLLTAVQKDYESQVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW57</accession>
    <name>U5VW57_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_14705</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361282.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14705"/>
    <dbReference type="EnsemblBacteria" id="AGZ41223">
        <property type="protein sequence ID" value="AGZ41223"/>
        <property type="gene ID" value="AFR_14705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2986"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_40_3_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="17"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="22522" checksum="A0FDA890D23F6C4B" modified="2014-01-22" version="1">MNLTETGRARNPRGEGARLRDEIVGAAVDLLDETGDERALTLRSIARRAGIAAPSIYRHFPDQPAIMLAVVRDAFAELHLALRAAVTGAGDDPRERLLALCHAYLHFAQSRPQRYRTMFGGLWTPTPGESSIVAEDLTSLGNESLALLAEALDACVATGTSLSTDPAGDAVAVWLGLHGFAHQRAVSHRLPGPADLGERMITALARLTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWF8</accession>
    <name>U5VWF8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_14150</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361171.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14150"/>
    <dbReference type="EnsemblBacteria" id="AGZ41112">
        <property type="protein sequence ID" value="AGZ41112"/>
        <property type="gene ID" value="AFR_14150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2871"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_2_11"/>
    <dbReference type="OrthoDB" id="1911275at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="13"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="23667" checksum="24DE9ED948AB2AC4" modified="2014-01-22" version="1">MPIPTGSPAVDRSLLRDDVYRRLRDAIIDGTFTPGEQLKDGELAEWLGVSRTPVREALLRLGSSGLVVALPGRSTRVSTIDAPAVRDARDVIAAMHELAVRQTTGRLSDDDIERMRAANRRFAEAVASGDVGAALDADEEIHRIPVAALGNRAIETVLDQFDPLVRRAERLRFRADGHASVDLHSHLIDLIAAGDASAAATLAFDIWHTLPTEPRTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWY1</accession>
    <name>U5VWY1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Adenosylcobinamide kinase</fullName>
            <ecNumber evidence="8">2.7.1.156</ecNumber>
            <ecNumber evidence="9">2.7.7.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Adenosylcobinamide-phosphate guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AFR_09285</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosylcob(III)inamide + ATP = adenosylcob(III)inamide phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15769"/>
            <dbReference type="ChEBI" id="CHEBI:2480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.156"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosylcob(III)inamide + GTP = adenosylcob(III)inamide phosphate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15765"/>
            <dbReference type="ChEBI" id="CHEBI:2480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="EC" id="2.7.1.156"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosylcob(III)inamide phosphate + GTP + H(+) = adenosylcob(III)inamide-GDP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22712"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.7.62"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 6/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CobU/CobP family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.156" evidence="8"/>
    <dbReference type="EC" id="2.7.7.62" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09285"/>
    <dbReference type="EnsemblBacteria" id="AGZ40146">
        <property type="protein sequence ID" value="AGZ40146"/>
        <property type="gene ID" value="AFR_09285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1891"/>
    <dbReference type="eggNOG" id="COG2038">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002982_0_2_11"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00236"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043752">
        <property type="term" value="F:adenosylcobinamide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008820">
        <property type="term" value="F:cobinamide phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003203">
        <property type="entry name" value="Cobinamide_kinase/P_G-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34848">
        <property type="entry name" value="PTHR34848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02283">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="SSF52733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="599"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="207"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00007490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ40146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="618" mass="62373" checksum="BC7A00B6BC6BF058" modified="2014-01-22" version="1">MLGGIRSGKSAFAEALVAGAASVRYVATSAGGEDDPEWRARIETHQRRRPQSWTTEETGADPAQLAAVLAGAKPDDTLLVDDLGGWVAALLDPAQQPNDDVATVTALAEAVRACAARVVVVSPEVGLSLVPATPVGRAFADALGTANQALAAACDRVAFVVAGRPTWLKTGADEPGIVTDLEAENAETAAPAEGLAVDTAAMHDAMTAATPTQPTSPAEETETRNEPLTPAEAIAAHRARTADAGALHAPTMALPLVASGTVIQPGMDMPLPDSDAGPDARDRLANVDLPGAGIGMLIEAVEFAAATQSTSTPRPWGPVRVLLIDGRHAGGAAAGADVEDSERRAAQAEAGEGALGTLAATADADIVVVRTPPSGAMEDGPVIDADTVDAALRQGWELADAAADAGRDVLILGSCGAGTEAAATAVLAATTGAEPVAILPRVLVPGGFYDDNAWMVRCAAVRDAMHRIRQEPRGAKDILAQLGGADLAVATGAMLGASARRMPVLVDGPLGIAAGLIARDISSQTRHWCLLADAGTHQLVKQGADVLGLTAVLELGLDLGEGTNALAALPLLRSAIALASALPVHPALLAGHGDAGLSADDDADFIEPEPDGPGPARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX00</accession>
    <name>U5VX00_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phospholipid-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0098 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361376.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15175"/>
    <dbReference type="EnsemblBacteria" id="AGZ41317">
        <property type="protein sequence ID" value="AGZ41317"/>
        <property type="gene ID" value="AFR_15175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3081"/>
    <dbReference type="eggNOG" id="COG1881">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083918_2_1_11"/>
    <dbReference type="OrthoDB" id="1150223at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289">
        <property type="entry name" value="PTHR30289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="SSF49777"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00481">
        <property type="entry name" value="TIGR00481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="180" mass="18283" checksum="8132E0956EBD1125" modified="2014-01-22" version="1">MTTLNDPFARLPEAASFTVTSSTITDGGVLPAEQMSGLFGVPGGKDISPQLSWTGAPDGTRGYAVTVYDPDAPTGSGFWHWAVADLPATVTDLPEGAGDDTGSGLPEGAFQLRGDAGAARFVGAAPPAGHGPHRYFVVVHALDVENIGIPADATPAVLGFTMFGHVLGRAVLTATAEIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXM3</accession>
    <name>U5VXM3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D,D-heptose 1,7-bisphosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_16605</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GmhB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361674.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16605"/>
    <dbReference type="EnsemblBacteria" id="AGZ41599">
        <property type="protein sequence ID" value="AGZ41599"/>
        <property type="gene ID" value="AFR_16605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3367"/>
    <dbReference type="eggNOG" id="COG0241">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0859">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_518416_0_0_11"/>
    <dbReference type="OrthoDB" id="1068323at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004446">
        <property type="entry name" value="Heptose_bisP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006543">
        <property type="entry name" value="Histidinol-phos"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42891">
        <property type="entry name" value="PTHR42891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01656">
        <property type="entry name" value="Histidinol-ppas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="519" mass="52658" checksum="C7A5D4A63921A748" modified="2014-01-22" version="1">MTALHDAVLFDRDGTLIADVPYNGDPALVTPMPGARAALDRLRAAGLRLGVVTNQSGLARGLFTPEQLAAVNARVEDLLGPFDTWQVCPHDDTAGCRCRKPAPGLVTAAAAALGTTPARCVVVGDIGRDMAAAAAAGAAGVLVPTPVTLPDEVAAAPAVVADLTAAADLILERQALVTATPRPRAGTVLVVRSDSAGDVLLTGPAIRAVAAGARRVVLLCGPRGRAAAELLPGVDAIIEERLPWIDPQPQPVDAGTLRRLTGKLAAVQADEAVVFTSFHQSALPLALLLRAAGTPRITAISEDYPGSLLDVRHRVPDGLPEAERARSVAAAAGFGLPAGDDGRLRVAVEPQERGGYLVVHPGASVTARACPPEVVRRIVDALTAAGHRVIVTAGPGEQGLADQVGGEPAGPTTLAGLARLIAGADCLVSANTGPAHLAAAVGTPVVSLYAPTVPFGQWGPYRVPHVRLGDAAAPCRNTRATICPVPGHPCLSGVAPSDVVAAVRLLGVPAPALSEEIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXT8</accession>
    <name>U5VXT8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4HBT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_10985</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360404.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40486">
        <property type="protein sequence ID" value="AGZ40486"/>
        <property type="gene ID" value="AFR_10985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2237"/>
    <dbReference type="eggNOG" id="COG5496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119426_3_0_11"/>
    <dbReference type="OrthoDB" id="1895484at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025540">
        <property type="entry name" value="FlK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36934">
        <property type="entry name" value="PTHR36934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014972">
        <property type="entry name" value="FlK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="3">
        <location>
            <begin position="38"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR014972-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR014972-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="131" mass="13740" checksum="72727F5A7F5440DB" modified="2014-01-22" version="1">MELPEFSPGLTARVELTVTDADTAQALGSGDVPVLGTPRVLALAEAATVAATARQIPGGVTTVGVRAEVEHRAPTPVGRMVTALATLAKVDGRKLIFDVVVRDGENLVAEVRVERMVLDRQRFIAKALGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXZ2</accession>
    <name>U5VXZ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_17380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361982.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17380"/>
    <dbReference type="EnsemblBacteria" id="AGZ41754">
        <property type="protein sequence ID" value="AGZ41754"/>
        <property type="gene ID" value="AFR_17380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3523"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="378"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="518"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="784" mass="83979" checksum="970D6CAF1236AFDB" modified="2014-01-22" version="1">MGTTQVSVLSPRSLLKDPALRVLGAITAAGFVLFLIFDGQQTRLAQTFWAFQVPLDAALALGGWRLRQAAPARFRRFWTMITFAGVSFTIGDSYHFLHTLFAAGVPSSTGGSVQTAFFAVGMSSNVIACLIFPQGLRTGREKFVFWLDATTVLVGGSVMAWCFAVNPIGDAHTDRVTASVTAALVIVAAFSATKVALIPEPPMARIAAWPMVGAALLQGVSAFLPGPFQEQDHAYGYALWLLPSLFIAAGPRIQEIVVRVGAHSGTAKRRPYSLLPYGMIALTFLVFFAMLPERVSSQLWGATAGVVVITCLVAGRQLVAFQDNMKLIRRLDVALNVLRDHEVLLRRQASFDGLTGLANRTHFSEELGRALAGLRTADEASVLLIDLDDFKTINDTMGHAAGDALLVTVAERLREAVGADDVVCRLGGDEFAVLLRSAGPATAMRTAGEILRGLAVPTEVQQHTIVTRASVGVAGASPGDEPSALLSNADIAMYEAKHRGKSTSVVYTDAMGARIGSEADLIRELAQAIESDQFSLVYQPIVRLDDMALTGVEALIRWNHPSRGLVSPIEFIPIAERTGQIVAIGAWALREACRQAADWRREFPSALNLTIGVNVAGRQLRTPGFADEVRGVLERTQLPAACLSIEVTETAVLDDEESHEAMLALRGLGVKLALDDFGTAASSLGLLLTCPVTTLKLDRSFVESITTVGRQSAVATAVSQMAHALEFSSVAEGIETDEQRELIQSLGYEYGQGYLFSRPLPPELMADLWAAAPRPARASAAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYW8</accession>
    <name>U5VYW8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360930.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12925"/>
    <dbReference type="EnsemblBacteria" id="AGZ40871">
        <property type="protein sequence ID" value="AGZ40871"/>
        <property type="gene ID" value="AFR_12925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2625"/>
    <dbReference type="eggNOG" id="COG2021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2730838_0_0_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="7249" checksum="D824D6947F16E48B" modified="2014-01-22" version="1">MVSRTSGVSRSSTSTATPRDSDPLVHPLRGRATAEAIPGATLVMVPGMGHDLPEGAWPTIADAIVANAARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYY3</accession>
    <name>U5VYY3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_18925</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362432.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18925"/>
    <dbReference type="EnsemblBacteria" id="AGZ42059">
        <property type="protein sequence ID" value="AGZ42059"/>
        <property type="gene ID" value="AFR_18925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3844"/>
    <dbReference type="eggNOG" id="COG5297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_302839_0_0_11"/>
    <dbReference type="OrthoDB" id="191946at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.870.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018366">
        <property type="entry name" value="CBM2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023309">
        <property type="entry name" value="Endo-1-4-beta-glucanase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027390">
        <property type="entry name" value="Endoglucanase_F_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000556">
        <property type="entry name" value="Glyco_hydro_48F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02011">
        <property type="entry name" value="Glyco_hydro_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00844">
        <property type="entry name" value="GLHYDRLASE48"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00561">
        <property type="entry name" value="CBM2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665363" evidence="7">
        <location>
            <begin position="37"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="8">
        <location>
            <begin position="33"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="429"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="974" mass="103644" checksum="6775F29CC0DC7250" modified="2014-01-22" version="1">MRSHSRRTRYRRGLAASAAVLLGAGLVVAGSSPAQAAAGCTVVYRVQSQWTGGFTGDIAITNSGDPVTSWRLEYDFPDANQKVTQGWSGTYAQSARHVTVSNAAWNGNLGTGASVSTGFNGTFTSANPVPTAFTLNGTPCNGAAAAPTVSISSPAANTRFSAPASIPMAATATAASGSTISKVEFYHDGLLLNTDTTAPYAYTWAGVPAQTAAYHLQAIAYDSAGVKSPTADVPVFVDASTTPAVITDATSLTVSPGKTATFKVALSKAPAENTTVTVARSAGATTVSATPASLTFTPANWNTAQTVTVAAATTAAVNSTATITASATGHTAASVTATVLAEGSADARFTQLYNDIKNPANGYFSPEGVPYHSIETLIDEAPDHGHETTSEAFSYWLWLEAEHGQVTGEWTPFNAAWASMEKYIIPSHADQPTNDKYDASKPATYASEHPLPSQYPSQLDPSVSVGTDPLAGELKSAYGTSDIYGMHWLMDVDNTYGFGRCGDGTSRVTYINTFQRGPQESTFETVPQPACDTFKHGGPNGYLDLFTKDSSYAKQWKYTNAPDADSRAVQAAYWALQWATAQGKQSQISAAVANAAKMGDYLRYSFYDKYFKNPGCTSTSCPAGSGKSSSNNLMSWYYAWGGATDTQAGWAWRIGSSTSHFGYQNPMAAYVLSNVSALTPKSPTAKADWQASLNRQLEFYQWLQSSEGGIAGGATNSWEGAYKTPPAGTPTFYGLSYVEAPVYEDPPSNRWFGMQTWSLERLAEYYYTTGDAKAKSVLDKWVTWALDNTEIGASSFAIPSDLAWTGKPATWNPSSPVANTGLHVEVTSKGQDLGVAGSYAKLLTYYAAKSGNARAKTAAKGLLDAIWAYKDPKGVSVTETRADYNRMDDVYDAATGQGIYIPPGWSGKMPNGDVIKPGVSFVDIRSFYKNDPDWPKVQSYLDGGPAPTFNYHRFWAQVDVATGYADFARLFPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ29</accession>
    <name>U5VZ29_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13225"/>
    <dbReference type="EnsemblBacteria" id="AGZ40931">
        <property type="protein sequence ID" value="AGZ40931"/>
        <property type="gene ID" value="AFR_13225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2685"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_25_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011075">
        <property type="entry name" value="TetR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16859">
        <property type="entry name" value="TetR_C_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="23012" checksum="CCAEE2FC9FBD73FE" modified="2014-01-22" version="1">MTQSTPFRRRRGAALEQALLDAAWAEISERGYEALNFDDVAARAGTSKPVVYRRWPSKAELARAAFRHAIGRDPLAVPDTGNLRDDVITLLRQVNEHRVGIATALIAQLGDLYRATGTSMEDLRASLAIGQETAMEQIVGRAVQRGEVDPAQLTDRIVQLPVDLFRHQLLMTARAVPDADIIEIVDTIFTPAVTRSTGAGFNKRNGPGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZS0</accession>
    <name>U5VZS0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gcvH</name>
        <name evidence="6" type="ORF">AFR_21080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="3">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362860.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21080"/>
    <dbReference type="EnsemblBacteria" id="AGZ42488">
        <property type="protein sequence ID" value="AGZ42488"/>
        <property type="gene ID" value="AFR_21080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4273"/>
    <dbReference type="eggNOG" id="COG0509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_2_11"/>
    <dbReference type="OrthoDB" id="1721828at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="PTHR11715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="22"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="3 4">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="13222" checksum="40C4A89F18BA4719" modified="2014-01-22" version="1">MIPEDLRYTAEHEWVSGDGSGPVRVGITHFAQDALGDIVFVQLPDEGTVVQAGDQLGEIESTKSVSEIYAPVAGTVVARNGSLGDEPELINAEPYAAGWLVEIAPDDPAALEGLLDAEAYKALTDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0C0</accession>
    <name>U5W0C0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bcr/CflA family efflux transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_15765</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Bcr/CmlA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361494.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15765"/>
    <dbReference type="EnsemblBacteria" id="AGZ41435">
        <property type="protein sequence ID" value="AGZ41435"/>
        <property type="gene ID" value="AFR_15765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3201"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_47_0_11"/>
    <dbReference type="OrthoDB" id="1452500at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004812">
        <property type="entry name" value="Efflux_drug-R_Bcr/CmlA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00710">
        <property type="entry name" value="efflux_Bcr_CflA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="6"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ41435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="393" mass="39940" checksum="6434EB954EC30A76" modified="2014-01-22" version="1">MLKGSTVLVLGLLTALGPLSIDLYLPAFPALRRDLDATDGTVQLTLAAMTLGLAAGELFMGAWSDRVGRRLPLLLSTSVHVAATIGCALAPSIAVLAAFRVVQGIGAAGSAVLVLAIIRDAADGRALVVLLSRVTLVTTTVPLLAPVAGAELLNLAGWRGIFAVLAAASAAVLLAGALGVPETHVPSPAPVRLRSRLRAVWSDRAFRRATLVAAMTYAGVYAYVAASPLLLQEVYGLGTRTYSIVFLVNSLGLVAGVQLSSLLTRRWDTARVLAGFTAVTVLAAVAIVPLQWAHTGTGGLLLCLWLFVAACGGCFPCATALALDGQGGQSGTATSLHGFTNFTAAGVISPVAGLIGITGPAPVAGVLMVTSGIALLSVVTVLRSGKRVEAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0K0</accession>
    <name>U5W0K0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inositol 2-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361520.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15895"/>
    <dbReference type="EnsemblBacteria" id="AGZ41461">
        <property type="protein sequence ID" value="AGZ41461"/>
        <property type="gene ID" value="AFR_15895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3227"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_10_2_11"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="13"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="357" mass="38556" checksum="B4C243FB0C9C8188" modified="2014-01-22" version="1">MSVTPPPASGPIGVAVVGAGYWGPNLVRNFQATPAFRLRRLCDLDVERAQRVLGGYSTVQVSAELDEVLADPEVHAVAIATPAGTHLPVAMAALEAGKHVLVEKPLAATYEEGRRLVEEADKRGLTLMCDHTYCYTPAVLRIRELLHSGELGELHFLDSVRINLGLVQRDIDVVWDLAPHDLSILDFVLPPGVSVTEVAAHGADGIGAGRACVAYLTLRLNTGAIAHIHVNWLSPVKIRTSIFGGSKRTLVWDDLNPSQRLAIYDRGVDVAAPEELGDEQRRDILVSYRSGDMVAPALTEREALRTMVEEYARAITTGTPALTDGRSGLRVLEILQAASRSLAEGGKMIKLDEGRMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W104</accession>
    <name>U5W104_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH-type transcriptional regulator betI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22135"/>
    <dbReference type="EnsemblBacteria" id="AGZ42697">
        <property type="protein sequence ID" value="AGZ42697"/>
        <property type="gene ID" value="AFR_22135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4487"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041347">
        <property type="entry name" value="MftR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17754">
        <property type="entry name" value="TetR_C_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="20894" checksum="66B65ED024C71FC1" modified="2014-01-22" version="1">MGLRERTRQAVQSDIAAAAMRLFLDHGFDAVTMDQIATEVGISRRSLFRYFATKEDIVLGDLAERGRIVQAALAARPEGEGPWEALRAALVVLTEQPAYTVETAIGISHMFHSTPSLRARHVEKQLQWMELLVPHIETRLGIEPGGAPDPRAQALVASALACLDAAVEAWARRGGEGDVEILYDQAVAAVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W193</accession>
    <name>U5W193_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361807.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16940"/>
    <dbReference type="EnsemblBacteria" id="AGZ41666">
        <property type="protein sequence ID" value="AGZ41666"/>
        <property type="gene ID" value="AFR_16940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3438"/>
    <dbReference type="eggNOG" id="ENOG502Z7JX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065811_1_0_11"/>
    <dbReference type="OrthoDB" id="1666716at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="208" mass="23020" checksum="3DC7BE2F627915CC" modified="2014-01-22" version="1">MKALLRAEAAMWRSMFVLIRRRPLDLGPGETPFGYLGVVKPILFVLIGLSVVEIPILDLIIKNVVPWPPARWIMLVISIWGLLWMIGFYASMKIHPHVTGPAGLRVRVGAGLDVTVPWEDVESVGKHYRSLPSSKSVQFEDDGDRRVLAVVAAGQTSVDVRLRRPTTFTLPKGPSEPVQELRLYADEPAGLVSAAREWLAPAEPSTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1A1</accession>
    <name>U5W1A1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transaldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23800"/>
    <dbReference type="EnsemblBacteria" id="AGZ43028">
        <property type="protein sequence ID" value="AGZ43028"/>
        <property type="gene ID" value="AFR_23800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4821"/>
    <dbReference type="eggNOG" id="COG0176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079764_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="PTHR10683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="216" mass="22494" checksum="91C573E63B02CFBF" modified="2014-01-22" version="1">MRLYADTADRSAAEGLLATGLFAGVTTNPTILRRAGSGVDDIPAIYRWATESGAREVFFQAWGEDTETLVERGRELRGLGDEVVVKLVASRAGTAACARLAADGVPTLLTAVYDPGQAMVAAAAGATYIAPYLRRLIAAGRDGHGDVLAMHELLAATGSGTKVLLASIPDVPSMVALARHGVDCFTMAPAIAEQFFADELTAEAVRTFEDAVAGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1B0</accession>
    <name>U5W1B0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363476.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23295"/>
    <dbReference type="EnsemblBacteria" id="AGZ42929">
        <property type="protein sequence ID" value="AGZ42929"/>
        <property type="gene ID" value="AFR_23295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4721"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_5_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="28379" checksum="880C14693FF9BB9E" modified="2014-01-22" version="1">MSTILITGATDGLGRGLAERLAAEGHDLLLHGRDRARLDAVAEAVGGKPVTLLADLSDLTQVARLADEVQSATDRLDVLISNAGIGSGEPDGRERRVSADGYELRFAVNYLAGFLLTEKLLPLLRRTGSARIVNVASLGQAPLDFDDLMLTRGYSGTRAYGQSKLAQIMSGFELAGRVPAAEVTVTSLHPATYMPTKMVLQEVGHQIDDLETGVAAVRRLAVGTDVAGVTGQFYDRQREARAHNQAYDAAARSELRRRSLELVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1C9</accession>
    <name>U5W1C9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endo/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363313.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22760"/>
    <dbReference type="EnsemblBacteria" id="AGZ42822">
        <property type="protein sequence ID" value="AGZ42822"/>
        <property type="gene ID" value="AFR_22760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4613"/>
    <dbReference type="eggNOG" id="COG3021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058628_0_0_11"/>
    <dbReference type="OrthoDB" id="1387978at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="97"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="31846" checksum="757D94389378E037" modified="2014-01-22" version="1">MVIGRWRKGSALIAVAAVGLSGVLALHRLVPGAAGTALDSGLPWLGLLVPALLGVAALRRSTRTFVVCLLPAVFWAAMFGPVLVPRSSADHHDLRVATLNLGAANASPELALRAVVAAGPDVLILQELTDRNLAAAQRELDGDWAYHEVTGTVGLWSTLPLSETGPVDIGIGWTRALRATVPTPDGPVRVYAAHLASARPAATAERDQTIAALAAAVAADESPRVLVAGDLNTTTTDRQFDTFAPLRDTQQEAGAGFGFTWPSDLPVLRPDHILQRGMSTQRSWVLRAPGSDHRAVVAELDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2J4</accession>
    <name>U5W2J4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-carboxy-cis,cis-muconate cycloisomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362498.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19270"/>
    <dbReference type="EnsemblBacteria" id="AGZ42126">
        <property type="protein sequence ID" value="AGZ42126"/>
        <property type="gene ID" value="AFR_19270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3914"/>
    <dbReference type="eggNOG" id="COG0015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_3_3_11"/>
    <dbReference type="OrthoDB" id="347727at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="2">
        <location>
            <begin position="417"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="491" mass="48886" checksum="DFE7E2716B2580CF" modified="2014-01-22" version="1">MRQSSSPSDGLFDGVLAAGPVHAATTDAAWVRALLDAEAALARAAARTGLATFADAAAVLTACAGLEVDPGALGAEAAATGTPVVPLVERIRAAVPGPAAALVHRGATSQDIMDTATMLVARRAVLALLADLGAAVDLTAELADRHRGTLMAGRTLLQQALPTTFGLVAAGWLTGLDAARRRLTEVRDHRLAAQLGGAAGTLAAYAPEAADGPEAAAAGPEAAAAGPETAAGPEAAAGPEASAGPEASAGPESAARPEADLGVLMLAAFSAELGLAEPDLPWHTERTRIADLAGALGSAAGAIAKVARDLILLAQTEVGEVAEGGDGGGSSTLPHKHNPVRAISAAAAAAQAPGLVATLLAAMPHEHQRAAGNWHAEWQPLRSLLRSTGSAAFQLRAALDGLRVDTGRMRANLDLTRGALLAERVAGALRPALGSEAHAVVAGAVARGDLAGDPAITAHLGPDRLAGLFDPAGYTGSAGALIDRALRAARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2L9</accession>
    <name>U5W2L9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_19765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19765"/>
    <dbReference type="EnsemblBacteria" id="AGZ42225">
        <property type="protein sequence ID" value="AGZ42225"/>
        <property type="gene ID" value="AFR_19765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4010"/>
    <dbReference type="eggNOG" id="COG0627">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059519_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="26310" checksum="8B53A413B867A6D6" modified="2014-01-22" version="1">MLATIAATAGVVTAGGLYRRYGGDEPVIPDVPLGDERLEQRHSVARGQDVYFYTAVPAGHGDGRGLPVLLVLHGASKTAKDFPALGLGRFATDSVRRGNAPFVLAGATGGRLAWRPSGADDPQKMVHEEIPAWCAERGFDTSRMSACGWSMGGFGALLLAATFPGFVQAVAAFSPAVSPGDPVFTQAGALRDVPVGLWCGTDDGLLPEVKALEKALPQPKAAGSYSEGRHNFGYWSTCLPAAFDLVARVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2W1</accession>
    <name>U5W2W1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_26025</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364203.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26025"/>
    <dbReference type="EnsemblBacteria" id="AGZ43469">
        <property type="protein sequence ID" value="AGZ43469"/>
        <property type="gene ID" value="AFR_26025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5276"/>
    <dbReference type="eggNOG" id="COG2409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005108_1_1_11"/>
    <dbReference type="OrthoDB" id="149255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="540"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="573"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="618"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="652"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="182"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="511"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="703"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="724" mass="74968" checksum="A49A1C3F88A58660" modified="2014-01-22" version="1">MATYLYRLGRFSFRRRKLILALWLAALALLGVGAATLSGPTSDTFSIPGTEAQEAMDLLSERFPGTAADGAQARVVFAAPSGEKLTDEANKAAIAQTVAQLKTGPDVAAVSDPLQGTINQAGTVAYAQVSFKVAAVALTESDREALTAAADTGRESGLAIEMGGDALEEQGGPGNSEILGILVAAVVLVITFGSLLAAGLPLLTAILGVAAAVLGIQIASGFVDLGSSTSTLALMLGIAVAIDYALFIVSRYRHEIVGGRNYEEAAARAVGTAGSAVTFAGLTVIIALAALAVVNIPFLTQMGLAAAFAVVIAVLIALTLLPALLGFSGKKIMGRRKETVSTKPAAGLRWARFVTRRPVAVLVVAVLGLLVLAIPALDMRLGLPGDNLASPETTQRKAYDMLTDGFGPGFNGPLTVVVDAAGSPDPKQAATEAATVIKGLPGVVTVSPPAFNPAGNTAILQVIPASAPDSAATETLVHTIRDQDSSLHLSVTGLTAVNIDMSAKMGAALLPYLAVIVALAFLLLMLLFRSVLVPLKAIGGFLLSVVATFGAVVAVFQWGWFGSLLGVEQAGPIISMLPIFLIGIVFGLAMDYEVFLVSRMREEHVHGAEPTQAVVNGFAHGARVVTAAAIIMVSVFAGFILSPESLIKSIGFALAAAILFDAFVVRMTIVPAVMTLLGRRAWWLPAWLDRILPDIDVEGDKLREIPADPEEPAHQPSDLVSAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W313</accession>
    <name>U5W313_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25715"/>
    <dbReference type="EnsemblBacteria" id="AGZ43407">
        <property type="protein sequence ID" value="AGZ43407"/>
        <property type="gene ID" value="AFR_25715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5213"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_3" evidence="1">
        <location>
            <begin position="77"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="32856" checksum="8990E829172030D3" modified="2014-01-22" version="1">MTDLSAVDPQLREPLPVIMAASPRPLTLEVLPVRRQLGRDLRLTDEQMTRGGRITIESRDGYLIVRPAGATGPLPGVVYLHGGGLMVGDERSDMPVVLDWVEQAGVAVVSVDYRLAPEHAYPAQVEDCHAALTWTAAHADDLGLDGRLILAGVSAGGGLAAATALLNRDRGGPELTGLLLVCPMLDDRTVDHEVRPYDTWSSVSNRTGWTALLGDARGGPDVPPYAAPARATDLSGLPPAFIDVGTADIFLAEDVAFAQRLWAAGSLADLHVWAGGYHGFDASVPDAAVSRSARAARIDWLRRLLQVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W347</accession>
    <name>U5W347_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SGNH hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_26835</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364512.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43628">
        <property type="protein sequence ID" value="AGZ43628"/>
        <property type="gene ID" value="AFR_26835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5438"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029872_1_0_11"/>
    <dbReference type="OrthoDB" id="1247435at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665651" evidence="2">
        <location>
            <begin position="23"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH_hydro" evidence="3">
        <location>
            <begin position="217"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="424" mass="44752" checksum="B66F02EBC15CE58A" modified="2014-01-22" version="1">MRRFSKILTASALLVAAVGAPASGSDRTARDRPAWSTAWATAPAAAVGGVEQGYAGFTIRNVVHTTTGGSSVRIHLSNRFGTQPVLMGHVTVAISAHTGGRRDGTIDASTGAAKAGTLRDVLFNGRGSITVPARAEVVSDPVRLRVPADQDLLVTTWTPQPSGTVTYHPAAMQDSFFTRGPADHAGDEAATAFTETTRVWHYVSGVDVSGGPGTVVALGDSITDGVTSTWGANRRWTDYLAARLATSPVPDYGVANSGISGNRVLLDSNAPNYTIYDSFGPSALTRLNWDVLDRAGARSVIVFEGINDIQQTPHQDDPEQIIAGLAQIATQAHSRGLRVVGATIMPWKGWDSWTPELEKTRVAVNEWIRDGGDGALDGLADFDAATRDPADPERMLPAFDSGDHLHPNDAGDKAMAAVVPLSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3E7</accession>
    <name>U5W3E7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative diaminopimelate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_21240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362892.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21240"/>
    <dbReference type="EnsemblBacteria" id="AGZ42520">
        <property type="protein sequence ID" value="AGZ42520"/>
        <property type="gene ID" value="AFR_21240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4307"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_3_11"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="2">
        <location>
            <begin position="21"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="3">
        <location>
            <begin position="28"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="382" mass="40755" checksum="B89415E524F3224B" modified="2014-01-22" version="1">MRPPEHIEAALRRQQRPVCAYVYDTAALRDRAAVVRAAFPPGTTLLYAVKANGNPHVVRTLAEVCDGLEVASGGELALAVAAGARRIVFGGPGKTDAELAAAVAAGAQVNVESAHELRRLDRAARAAGVVAEAAVRVNRAGPALTGSHAMTGTPTPFGVDEEALPGVLGLPLTSVRIIGFHLHAVSNNLDAAAHAGFITEALDWSVATAARHGVPLRLVNVGGGLGVDYLSDATIDLTALGQDLIVPDGVELIVEPGRILAADAGWYAAEVLDLKRTHGRWFAVLRGGTHHFRLPAAWGYSHPFTVLPVDEWPYEWDRPQIENTSVDAVGELCTPRDVLARDQHVERLRVGDILVFGRTGAYAWDISHHDFLRHPPPVFEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3H7</accession>
    <name>U5W3H7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364374.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26475"/>
    <dbReference type="EnsemblBacteria" id="AGZ43557">
        <property type="protein sequence ID" value="AGZ43557"/>
        <property type="gene ID" value="AFR_26475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5367"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_1_11"/>
    <dbReference type="OrthoDB" id="1167152at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="22553" checksum="CF9CF5C92B25B71E" modified="2014-01-22" version="1">MLFDMDGTLVDSEKVWDVALYELAARAGGTLSPAARHAMVGSSMATSMRILREDLGQPDRDEAADVEWIESRVEELFAQGLVWRPGALELLHAVRAAGLPTALVTSTGRRLVEIALNTLGRENFDAVVCGDEVSAPKPDPTPYLTAADLLGVKIAECVAIEDSPTGLASAVASGAAVLAVPAELELPPTDGVHLRDSLEGVDLAFLGSVLTSIHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3M5</accession>
    <name>U5W3M5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endo/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362942.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21500"/>
    <dbReference type="EnsemblBacteria" id="AGZ42570">
        <property type="protein sequence ID" value="AGZ42570"/>
        <property type="gene ID" value="AFR_21500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4361"/>
    <dbReference type="eggNOG" id="COG3568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060500_2_0_11"/>
    <dbReference type="OrthoDB" id="1387978at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28290" checksum="58FEB96CA9BB3D46" modified="2014-01-22" version="1">MRLATFNLLHGRSLSDGKVHAERVASAVTDLDADILGLQEVDRAQPRSGLLDLTAIAAEAMGAQVHRFAAAVVGTPGETWQPWRSDDDNAHPLYGIALISRWPVIRWQITTLPAAPIRSPIWAPGPGGGLLLLKDEPRVLIAAVIDTPTGPVTVANTHLSFVPGWNIRQLRAAVRALRTLPAPRILLGDLNMPSGPVRAFTGWRTLARKPTYPSTGPRAQLDHILADPRGAGLLGSVVQVRTPEVGISDHRPLIVHLDPPLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W411</accession>
    <name>U5W411_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560290.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28460"/>
    <dbReference type="EnsemblBacteria" id="AGZ43953">
        <property type="protein sequence ID" value="AGZ43953"/>
        <property type="gene ID" value="AFR_28460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5770"/>
    <dbReference type="HOGENOM" id="CLU_1923037_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025680">
        <property type="entry name" value="DddI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14430">
        <property type="entry name" value="Imm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="131" mass="14511" checksum="D498CC04D10ABF48" modified="2014-01-22" version="1">MRAKITWRSDEDGHLVTCEEEFRHALAEAEQDAARLPLIADITVGDGDCLSIGLGRAISVLSYVRASKEPPYLISQGSARVQDSEGVVFHYYGHWSEFPPSAAVRVKDAVEAVRHFCEHGELSSLLTWVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4M1</accession>
    <name>U5W4M1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560294.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28480"/>
    <dbReference type="EnsemblBacteria" id="AGZ43957">
        <property type="protein sequence ID" value="AGZ43957"/>
        <property type="gene ID" value="AFR_28480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5774"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3899">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_34_2_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="1429"/>
            <end position="1564"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1564" mass="167751" checksum="88294C4498D9520F" modified="2014-01-22" version="1">MLYRSERTRVLRVAGANGRTVVRKEYAGADAVIRARREADVLDRLAGTPGVVQRAGPAEPGVLLLQDCGDTTLASAVRAPGLHELGRRLAEVVASVHAAGVVHKDINPANVMLVDGAPVLIDFELATTAAEERPGFTHESMIAGTLAYLAPEQTGRTGRAVDHRSDLYALGATLYELATGQPPFGRHDPLRLIHDHLARVPEPAAAIEPATPPALSAVIARLLEKEPDRRYQSAEGLAYDLARAGGDLITLGERDFAARLAPPSRLVGRDSECDRLREIFAQAACGGSGAVLVSGPPGVGKSSLIDELRPPVTAAGGWLISGKFDQYRADARNAVTQALQGLGRLLLAEPEESLAELRDRLRSRTGADASQLVAMVPEFGALLGVEAAAPPDDRAEGQARLFAAGLGALRAVVSAERPVVMIIDDLQWAAATPVAFVEKLVEERVPGLLVVGAYREADLDPAHPFARLLDRWSRGPDGPATLRLANLPPGDIAALLAEMLRLPADRAAQLADAVGAHTAGNPFDTVEFVNALRRDGLLVAEDGGWNWDAAAVRTYVGDGDVVHLLAARIARLPRPARQLVKVMACLGAEVELPVLLTAYPVPDEWLGGRLEPALRDGLITTDDAVVRFRHDRVQQAAHDSLGTTGRRLLHLVLARRLRAAGESSLAAQQYLAAYPVVQDRDERLEVARLLATTAAGLKVVNQVTAERYLAGALALAGDDDQELRSAVLIDRHRTLCDLGRLAEADGLYREIVQRPDPLDLATATPAQITGLTVRGRPREAVDLGLAVLRLLGLDVPAPETIEAEVDRGLEELRDWAAGADRRSDAARAELSDPHMIAAAAIILRMMPPSFFGAQTTMSWLVVTAQRVWHDHGPSPQLIGSLGHAGIAAIVARSDYRTGYETLRHVTAVGEARGWAPETAVGRFLASVSTTHWFEPVENAVSLARQAREELLRSGDLQFAVATFHTSVPSSIDCAPDLVSYAAEVTAGVALAARSGNEQAAATTYQFRHLVSVLQGQPVSPGFDATAYERGRSTNPMAVAYEHTTRALAAAIFGDRSGLREHSAAAAGLSTYIGASYSTSTVHLLLALADGPEQHRRTARDWLAARAADAPDNFRHLVHFADAELAWTAGDHHAALRAFDAALNDLGNRCRPWHRALITERAALLHLQEGREFHGRRLLADAHDLYGTWGATAKLRALERAHPFLRTVTPSRTSVNVGHTTTLAAQDVDLMAILTASRALSSETRLDRLRDRVTDILGTLTGASHIRMLVREGTEWVLPAETSLPLTAVRYVQRTREPLLVADAARDDRFARDPYLAGLEFCSLLVIPVLIQGTPLAILVLENRLARAAFTADRLDAVLLIAGQLAVSIENARVYASLEATVAERTEELHRANEQLQILSSTDALTGLANRRQLDTRLATAWHDGAERHEPLSIAMIDVDHFKLYNDRYGHPAGDACLRRVAAALTGVIRTSDLVARYGGEEFALILPGADPATALAIAERARSAVLALTEPHAAAPAGLVTVSIGVASIVPGPDSGPQLLVDSADAELYQAKQHGRNRVMSDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4R9</accession>
    <name>U5W4R9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lipid ABC transporter ATPase/inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_23635</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23635"/>
    <dbReference type="EnsemblBacteria" id="AGZ42995">
        <property type="protein sequence ID" value="AGZ42995"/>
        <property type="gene ID" value="AFR_23635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4789"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="20"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="327"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="560" mass="57236" checksum="8E37D045132AFA47" modified="2014-01-22" version="1">MTQRQGLLGQALGGQRRWIVLSTLMITGHQAGEVAVPFLIGVVIDQAVTGDAGDLALWLGVIGAVYLGLSYSYRYGERFGERASEQAAHELRLRVTRRVLDDRGGAETDRLPGALVSIATSDAARVGAVAVAVSSGVAAVVGLSFAAVLLLSISVPLGLLVLLGTPPLMGLTRLLGKPLEERSETEQEHGAQASGTAADLVAGLRVVKGLRAGPAAVTRYRERSRGALAATVRAARAEAAYQGVVLSLSGLFLAVIAVAGGILAVRGEITVGQLISSVGLAQFLVGPMSTFGWVGADLAQGRASARRVAAVLDMPAAVTGGDASPSVPVRGAIRIHGLADPPLRALDLEITPGELVGIVAADPAEAAALVRLLGRSCDPAGGVIELDGVALTELAPDGLRSAVLSTEHHTDLFGGTIRSAVSLSGRARPETVEAAVITAGADEVAGGLPDGLDAVLDDGARSLSGGQRQRIALARALAADPPVLVVHDPTTAVDAVTESRIAGRLREMRAGRTTILVTTSPALLAVTDRVIVLHDGAVRADGTHGDLVAGDSTYRETVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4T4</accession>
    <name>U5W4T4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23710"/>
    <dbReference type="EnsemblBacteria" id="AGZ43010">
        <property type="protein sequence ID" value="AGZ43010"/>
        <property type="gene ID" value="AFR_23710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4804"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="42"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="32424" checksum="2156C1CE56DDA882" modified="2014-01-22" version="1">MSSIPDHISIWGELADVDHEVRHVDVGGVRTRVLRAGSGPDLVLLHGTGGHLEAYARDIGGLAADFRVTVYDMVGHGWTDLPDTPYTIDVLADHLLALMDALGIERAHLSGESLGGWVAAWTAAFHPERVDRLVLNTPGNIANKPEVMQRLRDSTMAAVRDPSEATVRPRVEFLFHHKEMVTDELVNLRRAVYSRPGFVRAITNTLVLQDPEVRKDFAWSPEWVGKISTTTLLLWTDHDPTGGLDEAALLLDWLPDARLHVIEDAGHWPQWEKPGEYLQVHRDFLLNGETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4W0</accession>
    <name>U5W4W0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Virulence factor mviN</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_29910</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560580.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29910"/>
    <dbReference type="EnsemblBacteria" id="AGZ44243">
        <property type="protein sequence ID" value="AGZ44243"/>
        <property type="gene ID" value="AFR_29910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6058"/>
    <dbReference type="eggNOG" id="COG0728">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006797_2_0_11"/>
    <dbReference type="OrthoDB" id="749401at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004268">
        <property type="entry name" value="MurJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03023">
        <property type="entry name" value="MurJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01806">
        <property type="entry name" value="VIRFACTRMVIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ44243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="552" mass="55228" checksum="C08C499E11E10EE9" modified="2014-01-22" version="1">MTDTPDQGQTATAGSEPAATAPRSGAGRIVGAAALIAGLTVLARIAGFGRTVVFISTVQANSLGDLYNAANTVPNIVFELVAGGALASLVVPLLAGAVAAGDRNRVNAVASALLTWTLVLLVPLAVLVALFAGHIVGLLPNMPPEAHEIGARMLRIFAPQLPLYGVGIVLTGVLQAHHRFAWPVIAPLLSSVTVMGAYLTYAVVDGRGTGLGKLTTGGELALSLGTTLGVVVLSLCLLIPLKGLRLRLRPALRFPGDAGRQVVRLGWAGAVTVGSQQLMVVVVLALAAGEGDLTSFNLAQALYLLPWAVLAVPLATAAYPTLAATAERGDEDGYRATLAGTARTVVLLACLGAAALVAVAGPVADLLDTPQAAPGIAGFAPGLLGYALFALLSRALYARGATSSAAAATAAGWALAAGAALVLVAVLPGDDKVLALALANSIGMLTLGALLVTAVRRRAGAAALAGLPRAAGVGIVAAVAAGLAGWGVVEALSPATPAGWVSVLSGMLGGVVVAVVFLAVTYPLDRHDLRPLVTRVTGRIRRTSAPDGKDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W554</accession>
    <name>U5W554_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Extracellular solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30095"/>
    <dbReference type="EnsemblBacteria" id="AGZ44279">
        <property type="protein sequence ID" value="AGZ44279"/>
        <property type="gene ID" value="AFR_30095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6096"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_10_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="432" mass="46294" checksum="C2AF5AE2E92B9201" modified="2014-01-22" version="1">MTRRALLSGTAAATALGLTGCAGLTPGRDTSGSLDFWNLFGGGDGVRMTQMLDGFRTANPGIELSAVTLAWGNPYYTKLSLATLGDKPPDVAISHLTRMKTLVASDLLEELRPEDLARHGIAADKFTERAWQAGLVDGKAYAIPIDTHPFVMFYNTDICEKAGLLDSSGALTPLDSPEKFLDAMKRAKEASGAYGGVVTVNNDTSTPWRIFQSLYSQLGGETLTDEGRRVVIDDAKATQVLGFLQSLTKDGLFPADIDYQGAIATFANGQAGFYFQGEWEISTFQTAKMPFSMTLFPNLYGGSTYAVQADSHTLVIPRQPKQDTAQLDRSLLLVRSMLDQSKTWAEGGHVPTWLPFRDSAEYQAMTPQSNYAAAADGAVYDPDGWYSGSGSNFEIVTGSAVATVLSGQAAPADALSRMRGQLETLSTTASPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5D1</accession>
    <name>U5W5D1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_30815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560758.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44423">
        <property type="protein sequence ID" value="AGZ44423"/>
        <property type="gene ID" value="AFR_30815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6238"/>
    <dbReference type="HOGENOM" id="CLU_1197736_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038637">
        <property type="entry name" value="NPCBM_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="22173" checksum="E8D0D38CD0C73E94" modified="2014-01-22" version="1">MPFERGSWSDALAAFAAVAALLIGFFGWQYNDDDKKDDKKQVAAPPSATAPAAPAVATPAVTSAGPAPPAAGVTGDLLTGVERIAGTAGELPDTLDPASYPDALVVECPSNQTGDQSRELKYNVDGRYRTFTADVSGWAEAKRPDDVQLRISAGTRQIDDTVITEELTAWTGRTNTPGGPITARIKGATELSLVVGCHKPGGVVIISNPRLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W667</accession>
    <name>U5W667_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32100"/>
    <dbReference type="EnsemblBacteria" id="AGZ44678">
        <property type="protein sequence ID" value="AGZ44678"/>
        <property type="gene ID" value="AFR_32100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6499"/>
    <dbReference type="eggNOG" id="COG3568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010649_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28517" checksum="97F9112F152EFB71" modified="2014-01-22" version="1">MTWNVWWRFGGNWRERATGIKITLETYQPDVIGLVETWAGNGTTQPDELARILGLHAAFAPTSLPPAPDPIEHPDQAGVTVGLGLLSRWPILRTRVHELPHPQRGGEPPTALVATLDHPRGELHVIVTCREWEPHHAADRLAQSEALAELIMDPALDGPLPVLLVGDLNAPPDEPELAPLREVTVDTWEAGGGDPGVTTLDSLLPYAPLEATTLIDRRIDHVLVRPGRPGVAVTVRGAFIAGDRPIDGHYPSDHYAIGADLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6K0</accession>
    <name>U5W6K0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA polymerase sigma factor rpoD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_31860</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560965.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31860"/>
    <dbReference type="EnsemblBacteria" id="AGZ44632">
        <property type="protein sequence ID" value="AGZ44632"/>
        <property type="gene ID" value="AFR_31860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6449"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_1_11"/>
    <dbReference type="OrthoDB" id="1857862at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012845">
        <property type="entry name" value="RNA_pol_sigma_FliA_WhiG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02479">
        <property type="entry name" value="FliA_WhiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="30"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r3" evidence="5">
        <location>
            <begin position="117"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="7">
        <location>
            <begin position="195"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="33464" checksum="629CC61BD43244CB" modified="2014-01-22" version="1">MTSTMTTAADLAADTATAVPALSAREQEELIRTHMPLVGHLVRDMLSRIPNHIHRDDLTSAGLHALVTAARGWDQTRGIPFHRFATTRIRGAILDELRSLDWATRSVRTKARATDTTRQQLTTTLGRTPTNEELAQALGTTTTDLHQTDNDVQRATVLSLQGFTTSSADDLVTERTPGPEDMLIRREQIGYLHHAIAALPERLQVVITEYFLKERPMADIAADLGVTESRISQLRAEALSLLKDGLNTHLDPELAPAPENPESITARRRASYYANIAGNTNLHSRLAMTNAQGFTPLATPVRTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6K4</accession>
    <name>U5W6K4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364468.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26715"/>
    <dbReference type="EnsemblBacteria" id="AGZ43605">
        <property type="protein sequence ID" value="AGZ43605"/>
        <property type="gene ID" value="AFR_26715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5414"/>
    <dbReference type="eggNOG" id="ENOG5032CIK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1187908_0_0_11"/>
    <dbReference type="OrthoDB" id="1902765at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="237" mass="24698" checksum="DC96E669DBDAE07C" modified="2014-01-22" version="1">MTTSDTDGTLERRAPDYRSVVRKLTNLDDEAATHRAEARTWHDTRAIEADNAVRAADQRIRETQAAVRAAQRARDEVDARAAGLWADFAHKTGRLADRFGAQAPQPTIPRQRDRDAEDYLQEATALAGATPAARPASGSASLLYAVLGALGGAVGVAAWSLLRWAGREAGGDLAVGLPVVALIVALLGPILAVVTAKRVADRRSVQLDASAVATVLIASLIAAGLVVALARGATAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6L6</accession>
    <name>U5W6L6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Family 5 extracellular solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560980.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31935"/>
    <dbReference type="EnsemblBacteria" id="AGZ44647">
        <property type="protein sequence ID" value="AGZ44647"/>
        <property type="gene ID" value="AFR_31935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6464"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_8_3_11"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666388" evidence="1">
        <location>
            <begin position="22"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="90"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="561" mass="60649" checksum="A872C8EBBF2768D0" modified="2014-01-22" version="1">MQRRKLFAVALAGVLASGGLAACSGDSPNAGNKNASGGATFLTVGMPNGPQTENHNPFLGTSAGASLGYRWMLYEPLMMWNPVKPADPSKPWLATGAEWSPDFKKVTITVRDNATWSDGQKLTAEDVAYTFNLIKTNEALNSAATPYGEITASGSTVTVTFTNSMFVYKDKWLGQTPIVPKHIWEGIKDPATDANKTPVGSGPYTLKSFTPQTTTLSVRTEGYWQDLPQVKELRYTSYTDNNAQTTALSDGSAEWSFVFIPNYKTVFIDKDPAHYKVWAPPVLGIHGLYINTTKAPFDNVALRKAMSMVVNREDIFNQAEAGYFHPLVTSVTGLPSPAGDAFVAEEYKGKNQTVDVEGAKKVLADAGFKLNGTTLNDPSGKPVKITLTDPSGWSDYQTSLEIVKDNFAQIGIAATVDKANQDAWFRNVEEGKFDATFRWTNGGSTPYDIYQTIMDGKVLKKIGTASPGGNFGRFNSPEATTALAAYANATDDAARKTAMNTIQKIFVEQVPMIPVGADNIGMAYSTKNWVGWPDDTNTYGAGQPTQANALDVVLHLKPANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6L9</accession>
    <name>U5W6L9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Endo-1,3(4)-beta-glucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_32705</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561137.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32705"/>
    <dbReference type="EnsemblBacteria" id="AGZ44799">
        <property type="protein sequence ID" value="AGZ44799"/>
        <property type="gene ID" value="AFR_32705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6619"/>
    <dbReference type="eggNOG" id="COG5498">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005482_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003610">
        <property type="entry name" value="CBM_fam5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036573">
        <property type="entry name" value="CBM_sf_5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="PTHR31983"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00495">
        <property type="entry name" value="ChtBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51055">
        <property type="entry name" value="SSF51055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666130" evidence="3">
        <location>
            <begin position="49"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="4">
        <location>
            <begin position="810"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="787"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="950"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="787"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="952"/>
            <end position="972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1023" mass="108015" checksum="BCC762962BC77722" modified="2014-01-22" version="1">MKSPLPPSRSPGTHRRPRRGRLAGFAAAVVVALGSGALAVAVVGNAEAATIGAGSYTETLPAGAKLPSGCGNISTNPRQYLTPNAPAGPVPTNDWWSSLLFKKGDECNFSQPLFAGPAAYKPVAGGLGISYQTDAAISGSATGLGEYHYPYAQHVVAGVAGLNAGQALVDGWSDWTVTPSWSDGSRTLKATIGHGLPLSYYQVTGGDALLTITGPPTAWQNTGNRIGFSIGGHDYVAYGPAGSTWTLSGSTIRSNLAGKGYFSVAVLPTLASAATADKIALADQYGQYAHNHVTGTKMSYTYNQGAGTVNTTYAFTTTAREGSASGTVIALYPHQWRYLTGSTPLSRTYVSPRGAMKIVLGTNFTTSMKYTGVLPEIPAVGDSSGADLSTITTYLNNELNNPADFKGDDTYWTGKGLGRAARIAEIADQLNLTSVRDNALNVIRTRLTDWFTASAGKSSRVFYYDRNWGTLIGYPASYASDEDLNDHHFHYGYYVAAAATLAKFDPAWAAPGQYGGMVDLLIRDANNYDRNDTRFPYLRDFDIYAGHDWASGHGAFGAGNNQESSSEGQNFANALIQWGQATGNTTVRDAGIWLHTTQAAAINEYWFDVRNENFPANWGHNYSAIVWGSGGAYATWFSGEHAMIVGINMLPMTGGQLYLGDYPASVRSTYAELVRNNGGEPTVWRDMLWQYLALGDPDAALAKFRANSSYTSEEGESKAHTFHWIRNLAALGQVDTGVTANHPLAKVFSKNGAKTYVASNITNAALTVTYSDGKTLSVPAGKTVANGATNWSGGNATGGGTTTPPTTPPTTPPTTPPCNGALLSQGKTATSSSVEAGYPADLAVDGNATTRWSSAFADPQWLQVDLGSAQPLGRVELSWEAAFGKAYQIQTSTNGTTWANAVTVTAGTGGNTSHALSGTARYVRMNGTERGTPYGYSLFKFKVFGSTCGTTPPPPVTTPPTTTPPATTPPATTPPSATAWAINTAYTAGRVVTYNGVSYRCLQAHTSLTGWEPPNVPALWARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6N8</accession>
    <name>U5W6N8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32805"/>
    <dbReference type="EnsemblBacteria" id="AGZ44819">
        <property type="protein sequence ID" value="AGZ44819"/>
        <property type="gene ID" value="AFR_32805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6639"/>
    <dbReference type="eggNOG" id="COG3647">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087528_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014509">
        <property type="entry name" value="UCP020606"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09997">
        <property type="entry name" value="DUF2238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020606">
        <property type="entry name" value="UCP020606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="207" mass="23197" checksum="273394E22254F482" modified="2014-01-22" version="1">MLVLGATWWRPIYPVEQALHHSLTVAGVIALVLVQRRRLLPYSSFLLILIFLTLHSVAARWIYSFVPYDDWTQALFGMRLSEVLGWERNHFDRLVHLSYGLCFGPVLFRRLLDAGLRVRWAAPTAVELVLSTSAVYELFEWAIAMTLAPGAAEAYNGQQGDMWDAHKDMALATGGAVLAVTIAVLLLRRRSGPAVTDRIRPPTVIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6R0</accession>
    <name>U5W6R0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative PfkB-family carbohydrate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_32905</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561176.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32905"/>
    <dbReference type="EnsemblBacteria" id="AGZ44839">
        <property type="protein sequence ID" value="AGZ44839"/>
        <property type="gene ID" value="AFR_32905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6659"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_6_0_11"/>
    <dbReference type="OrthoDB" id="1604782at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="5">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="31865" checksum="006EBDB13EB8B46F" modified="2014-01-22" version="1">MTGLVTLGETMGLVAADSIGPLEYARSFSLGIGGAESNVAIGVARLGGTATWLGRVGPDSTGDLIERRLRGEGVHAMVIRDTAFTGLMMKHRRTSGLLHVDYHRTGSAGSRLLPTDVPVDLVREAGILHVTGITPALSDSARATVFHAVETAAAAGVTVSVDVNYRGKLWSRYDAAPVLRDLVSRADIVFAGPDEAELILGSGSSVADGLAALGPSEVVIKDGARGCTALISGERHEVKALEVTLVDPVGAGDAFVAGYLADRLAGAGPSERLSTAIATGAFAVTVPGDCEGLPTRADLSSLLSATDNVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>U5W722</accession>
    <name>U5W722_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27620"/>
    <dbReference type="EnsemblBacteria" id="AGZ43785">
        <property type="protein sequence ID" value="AGZ43785"/>
        <property type="gene ID" value="AFR_27620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5598"/>
    <dbReference type="HOGENOM" id="CLU_1912569_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="132" mass="12997" checksum="B9A76644E8199BB2" modified="2014-01-22" version="1">MAGSTASTASSRWTGLLMWLLPPLFELPVVVALCSGVPEVAREAVFGAPGTQVVVLLAFVASVGGFVAAARGTSGLVQAGIAGILAIAAGVVAALGAGFLTGGGFLVLGLLLVHSAVSIAMLARATLRRTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W748</accession>
    <name>U5W748_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32845"/>
    <dbReference type="EnsemblBacteria" id="AGZ44827">
        <property type="protein sequence ID" value="AGZ44827"/>
        <property type="gene ID" value="AFR_32845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6647"/>
    <dbReference type="eggNOG" id="ENOG502Z9VM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043113_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="469" mass="49717" checksum="6ECCB1441A6AC7BE" modified="2014-01-22" version="1">MNEVVFPRTADGRRSSSALGRAVVADALRVADPAAAAAAEGERDWRKGYLRPFRALVEAGLDSGYDLARAGLDSVHERMRVVRDGEDVPLAEAFDIKAEAPPTVTVTGEGSRERELAIPYRGELLRGDTLHRQLDRWVERGVIEASCAEMVREVAAEPDWLNLSDQRIVVLGAGAEMGPLPAVLAWGGTVVGLDLPRPQLWARVAGTARKSAGTLIAPGSSVEDAGIDLLTGLPAAASWLLEREGRLVLGNYVYAPGAAYARLSAAVDALGVHVREQRPETALAFLATPTDAFAVPGDAVAQATSRFRERSAAARTTGALSGGRLLQPNYPEGADPGIADSLVPQQGPNYALAKRIQRWRASVSRQDGVPVSFSVAPPTRTRSVTSNRLLAAAYAGAHLFGVEIFAPATSNRLMAALMVHQLRKPRPAAPSAWVDETVGAAHGGLWRTAYQPRTALGLAAVRGLPAAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7A6</accession>
    <name>U5W7A6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbonic anhydrase</fullName>
            <ecNumber evidence="4">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_33170</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible hydration of carbon dioxide to form bicarbonate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + hydrogencarbonate = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33170"/>
    <dbReference type="EnsemblBacteria" id="AGZ44892">
        <property type="protein sequence ID" value="AGZ44892"/>
        <property type="gene ID" value="AFR_33170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6712"/>
    <dbReference type="eggNOG" id="COG0288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003182_11_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="PTHR11814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="SSF53056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00704">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="359"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfate_transp" evidence="12">
        <location>
            <begin position="5"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ44892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="704" mass="72880" checksum="A472019DA4E8953B" modified="2014-01-22" version="1">MRTLLRYDLPASVVVFLIAIPLSLGIAAASGAPLLAGLVAAVVGGVVAGALSGAPLQVSGPAAGLTVIVAGAVTDFGWAQTTALVAMAGLVQIVLGVSRVGRAALALSPAVVHGMLAGIGIVIALSQIHVVLGGDPQSEAWNNIRDLPAQIVGNHGASILIGVLTITILLVWPRIVKISLLPGALIAVAVATATAALAGFDVARVALPDNPLGELTFPRWPDAAASDIIIAVVTIALVASVESLLSAVAVDKMHDGPRADLNRELLAQGAANTVSGALGGLPVTGVIVRSSTNVAAGAKTRASAIMHGVWIAVCVLIFAALLEQIPLSALAAVLVVVGLRLVSLAQIKAYVRHRELPTYLVTALGVIFTDLLTGVALGMATAVLLMLGRLARCEIHRSSPAPGNWLVVITGTLAFVGSGRVARELAKLPPRETVELELHLDYLDHGAFETIQDWRDAYERGGGQVHIEEVQDSWFHRATSGRLGVNRSQPGRVPRFLGSWSQWISLNEQRRGAMAAGIDEFERSVAPIVRPHLAGLARDGQKPEQLFITCADSRVVPNMITTSGPGDLFCVRNVGNIVPAHGAGDDSVGASVEYAVDVLGVGTITVCGHSGCGAVGAMMAGAAGTPSALGSWLGSAGIDLTGRDHEEECIAANVEQQLANLRTYPSVRKAVDEDRLTLTGLYFDLAEARMYTVTDGVRSPVSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7H6</accession>
    <name>U5W7H6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561304.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33545"/>
    <dbReference type="EnsemblBacteria" id="AGZ44967">
        <property type="protein sequence ID" value="AGZ44967"/>
        <property type="gene ID" value="AFR_33545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6789"/>
    <dbReference type="eggNOG" id="ENOG50337XG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136722_0_0_11"/>
    <dbReference type="OrthoDB" id="2074700at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="146" mass="15406" checksum="0587B325CA3F40BC" modified="2014-01-22" version="1">MPEIRPARRWPIALLRTLTTIVLLQVLIQGALAGGFVTGDVNLLGLHSANAILLILTSTALLPATILLVRPGRGPWWPIVFGVVLWWAIVMQAGLGFARQVGLHIPLGMAIMGLTAALCWWAFAYRTPAPVPAGGPSAPFTAGGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7I8</accession>
    <name>U5W7I8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fimbriae Z protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561314.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33595"/>
    <dbReference type="EnsemblBacteria" id="AGZ44977">
        <property type="protein sequence ID" value="AGZ44977"/>
        <property type="gene ID" value="AFR_33595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6799"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_0_2_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="851"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="924" mass="98154" checksum="4352935F5CFD76C0" modified="2014-01-22" version="1">MRAPSPVLVGRDDLLALADRRLDAAASGRGGLLFLAGEAGIGKTRLLAAISARAAARGFTVAGAGAFPSDADVAGALIADALSLFGTRTAELLRRAYDGDSHRQRRLLVTDLADALAEIGAGPEPLLLTLEDLHWADDLTCEALARLARRLPALPMLVVGTYRSDELYPRVPIREWRTRLLNQRHAEEARLSRLGPEDTAVLAAAISDAVLPGAVTGAIFARSDGIPLHVEEFLATVGDATGLPETLAEAVLSRAAALSAPARDLAGAASVIGRSFDVDLLTAITGDSPHLVDDGLRELAERFFVSPHPNKITYDFRHALIRDALLADLPPHHRRDLHARAAGAAIAAGLPGAFVSDQFEQAGRPADAYTYALAAAGEAVEMSAHREAVELYRRSLRTVPVATPASTRAGLLTRYAAELAAVDENTEAVETLLRAYRMRLDLGDDLSAAALVPDLVAARHLLGAGLDERLRLLREGLALIEARSDEPAQEIRAGLHAALAAAYMLDRRLEEATEVGVLAEAFSVEDCDHQLRCHLGGTLGSILVFGGRMHEGWQMLENAVTRARRTGLEAEAARGYRMLGSSASVLVEYDLAREWLGAGIEYAESVERFNDRHYMTAHLAHVDWAEGSWPAAHAGAAHALADGQGITTRITALHVLGFVALGRGEWDSAERSLTEAAALGAAMGELQRVSPAWWGLASVAQLRGDSTAAIAWCERGYAASAKVRDAAYLFPYVVTGVRAYLAAQDLTGARDWLARTSALLRDRAIPGTLGAIDHGAGLVLLHEGQTGKARVALSEAAAFWDGRRRFWEGTQALLDQARCATRSRRPADAFSTLAASRMVSAGAAALSTTPPSPTGTLLTSREAEVARLVSEGLTNREIAAALTISPKTAAAHIEHILTKLGASRRAQIATWAALSTERGTSNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7N2</accession>
    <name>U5W7N2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561540.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34735"/>
    <dbReference type="EnsemblBacteria" id="AGZ45203">
        <property type="protein sequence ID" value="AGZ45203"/>
        <property type="gene ID" value="AFR_34735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7028"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_11"/>
    <dbReference type="OrthoDB" id="1015470at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="22"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="437"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="530" mass="57308" checksum="6E3DB9089AE5D109" modified="2014-01-22" version="1">MLSYASGISDVPLLGETIGANLERTVAAHGDREALVDVPSGRRWTYTEFDAAVNDAARGLLARGIAKGDRVGIWAPNCAEWVITQYATAKIGAILVNINPAYRTHELAYVVEQAGLSLLISAVAFKTSDYRGMIAEIGFSDTIFIGTPGWDALTAAGHGLSLDERAATLTFDDPINIQYTSGTTGFPKGATLSHHNILNNGFFVGELVGYTEADRICIPVPLYHCFGMVMGNLAATSHGACMVLPAQGFDAVASLRAVALESCTSLYGVPTMFIAELGLPDFADHDLSSLRTGIMAGSPCPVEVMKRVVAEMNMTEVAICYGMTETSPVSTMTRRDDGLDRRTATVGRVMPHLESKVVDPATGLPLPRGEAGELCTRGYSVMLGYWDQPDATAEAIDQARWMHTGDLATMDADGYVNIVGRIKDLVIRGGENVYPREVEEFLYTHPDIADVQVIGVPDQKYGEELMAWIILRPDASPLTAADIRTFCTGKLSHFKIPRYVHLVDSFPMTVTGKVRKVEMREQATDLLSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7N6</accession>
    <name>U5W7N6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_28500</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28500"/>
    <dbReference type="EnsemblBacteria" id="AGZ43961">
        <property type="protein sequence ID" value="AGZ43961"/>
        <property type="gene ID" value="AFR_28500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5778"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="79"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="140"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="359" mass="38699" checksum="12AB423D758E0394" modified="2014-01-22" version="1">MLCVIVLVGAQFVPDAAEFLRELWREFGPSWCAVPDLRAPGTDTSFVCAQVFRRPSLVTIGTRGLILLIFLAACLPLVRWCLRPLRELTPVIANVGPQNLGHRLRPGPGGDELAVLGRTIDEMMDRIAVGYEAQRRFAADAAHELRTPLAVQRILIEVGMAGTLTEDQLTLLTAQLLETNERNERLIEGLLVLSESDRGLLSRTPLRLDLITAEVLDAHRSLAAAAGITITSALLPRVVMGEQALLERLVTNLVQNAVKYNRPDGTLDVRVGDDPALIVENTGDEVPADKISGLFEPFRRLSGTRIDHSGGAGLGLAIARSITRAHDGIIAASPHGKDGLHVQVSFPAPDEAAGPATRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8A7</accession>
    <name>U5W8A7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative glutamate ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_35615</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561715.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35615"/>
    <dbReference type="EnsemblBacteria" id="AGZ45379">
        <property type="protein sequence ID" value="AGZ45379"/>
        <property type="gene ID" value="AFR_35615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7207"/>
    <dbReference type="eggNOG" id="COG0765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_1_0_11"/>
    <dbReference type="OrthoDB" id="1523519at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="17"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="23788" checksum="D095FAC7740BE70A" modified="2014-01-22" version="1">MDVFSDPTNIDVYVTGFLWILKLTAAGTVGALVLGVLLAAMRVSPVPVLRLVGTTWVNTFRNTPLTLIVFFCYFGLFSTLGVTLSDDIDTNNYWLGVVGLSVYTAAFVCEAIRSGINTVPAGQAEAARAIGMSFFQTLTMIILPQAGRAVIAPLGSIFIALAKNATIVGTIGLMESSNAMKELINANGDAVIPIFLVFAGTFALVLIPTGYGFGWLANRLAVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8J4</accession>
    <name>U5W8J4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36040"/>
    <dbReference type="EnsemblBacteria" id="AGZ45464">
        <property type="protein sequence ID" value="AGZ45464"/>
        <property type="gene ID" value="AFR_36040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7295"/>
    <dbReference type="eggNOG" id="COG2071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030756_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044668">
        <property type="entry name" value="PuuD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43235">
        <property type="entry name" value="PTHR43235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24644" checksum="443C495950E60BC6" modified="2014-01-22" version="1">MGKMRPVIGITTYVEPATWGVWHELPTTLVPHDYVAAVSAAGGQAVLIPHDDLDPDILRVLDGLVLSGGSDLGPALYGAEAGPKTVTRPERDVAEMRLTRAALARDLPILGVCRGMQVLTVAAGGTLHQHLPDVVGHDRHRPAPGVYGKHEARFTEGSRIAALMGDDTRVHCFHHQAVDDPGTLVVTGRAEDGLPEAVEDPSHKFVLGVQWHPEVTRDKRLFGALVTAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8L9</accession>
    <name>U5W8L9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MMPL domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_36165</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561825.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36165"/>
    <dbReference type="EnsemblBacteria" id="AGZ45489">
        <property type="protein sequence ID" value="AGZ45489"/>
        <property type="gene ID" value="AFR_36165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7318"/>
    <dbReference type="eggNOG" id="COG2409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005108_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="572"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="613"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="641"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="232"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="709" mass="74891" checksum="4DEBB98880B43C48" modified="2014-01-22" version="1">MFGWWGRAVVRLRWWVVAAGLAVVVAGATWGAGVFGSLSGGGYDDPDSESNRAAAAIAEQLGQRDPDLIILWTSAGVPVTDASVREAVTGVVTKLRASPDVAQVVDYTTQKSFVSNDQRSTYAAVYLETEDVDQKTAAYHRIEPDFAAPGLTTQVGGLVAFNDQANTQTEEDITRAEIFSLPVLFVLLVLIFRGLIAALSPLVIGGIAILGAFVAVRALTYVTDISVFAINVITLLGLGMAVDYSLFVVSRFREELANGKDVPAAIRTTVATAGRTVMVSGLTIVLALSSLLIFPQPFLGGMAIGGMAAVLVAMIAALTVLPAALSLLGHRIDKLRIPLPQRRHPGAGWARIAHSVMRRPILYVVGVLVVLTAFAAPFLRVEFGGFDERVLPADTSARIVGERLNADFPGGGENPINVLVSGGPADAFADRIAALPDVKGAAVTAEQGGSSLITVTYPSTLPAAESRDIVSAIRELPPPAGAEVLVGGRPAIDLDLLESLADRLPWMALIMAVATMLLLFLAFGSVLLPIKAVLMNLVSIGASFGVVVWIFQDGHLEGLLRFESTGFIEPSNMILVLAVLFGLATDYEVFLLSRVREEWDRTGDNTASVAAGLQHTGGIITAAALLLITVVAGFATGGVTVIKTIGVGMIVAIIVDATLVRALLVPATMRLLGRWNWWAPGPLGRFYARYGIHETEPAPADRPRETAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8S4</accession>
    <name>U5W8S4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AFR_35805</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561753.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35805"/>
    <dbReference type="EnsemblBacteria" id="AGZ45417">
        <property type="protein sequence ID" value="AGZ45417"/>
        <property type="gene ID" value="AFR_35805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7247"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051314_2_0_11"/>
    <dbReference type="OrthoDB" id="1702107at2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00560">
        <property type="entry name" value="pgsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ45417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="21316" checksum="BBED7B89AC4ADCA6" modified="2014-01-22" version="1">MTDEPVPVAAPRPVSLYNPANVLTAARIVLVPVFVAFVVVSGMAESGWRMAACLTFCFASATDFADGWIARRYELVTSFGKVADPIADKALTGSALVLLSAYDRLSWWVTGLILVREWGVTALRFWVIRYGVIPASRGGKLKTALQIAAIAWFLWPVPEPFDLIGTLLMIAALIVTVVTGVDYVFQALRLRRRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8W7</accession>
    <name>U5W8W7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560775.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30900"/>
    <dbReference type="EnsemblBacteria" id="AGZ44440">
        <property type="protein sequence ID" value="AGZ44440"/>
        <property type="gene ID" value="AFR_30900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6255"/>
    <dbReference type="eggNOG" id="COG0412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1142046_0_0_11"/>
    <dbReference type="OrthoDB" id="551171at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="26944" checksum="48281F99D97FDA09" modified="2014-01-22" version="1">MAEEPTFLRPFVLSPPAREPERTGQVDTYVPDGDGPFPVVLLVHGGPLPPELRPTPRDWPVFQGYAAALAEQGILASVVDHRLYAVPQDDGVLFDYVTAAADVAEAVETVRADKRADPARVVLWFFSGGGLLSAEWLRAAPEWLRGVALTYPLVVPRADGSTDPRFGPVKDVLGEGVELPPILLTRAGLEDPRLAVPLEEFVAAAGVPMEIVDVPNGHHSFDMLDHTEESRAAVRRAIGWVGERLGQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Y3</accession>
    <name>U5W8Y3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">D-alanine--D-alanine ligase</fullName>
            <ecNumber evidence="7">6.3.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">D-Ala-D-Ala ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">D-alanylalanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">ddl</name>
        <name evidence="11" type="ORF">AFR_36615</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the D-alanine--D-alanine ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.4" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561915.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36615"/>
    <dbReference type="EnsemblBacteria" id="AGZ45579">
        <property type="protein sequence ID" value="AGZ45579"/>
        <property type="gene ID" value="AFR_36615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7407"/>
    <dbReference type="eggNOG" id="COG1181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039268_0_1_11"/>
    <dbReference type="OrthoDB" id="764798at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00047">
        <property type="entry name" value="Dala_Dala_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000291">
        <property type="entry name" value="D-Ala_lig_Van_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005905">
        <property type="entry name" value="D_ala_D_ala"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011127">
        <property type="entry name" value="Dala_Dala_lig_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01820">
        <property type="entry name" value="Dala_Dala_lig_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039102">
        <property type="entry name" value="Ddl/VanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01205">
        <property type="entry name" value="D_ala_D_alaTIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00843">
        <property type="entry name" value="DALA_DALA_LIGASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00844">
        <property type="entry name" value="DALA_DALA_LIGASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 7 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="10">
        <location>
            <begin position="152"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR039102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ45579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="367" mass="38479" checksum="803BDB9BDB7DED65" modified="2014-01-22" version="1">MTTPRKTRVAIVFGGRSTEHAISCVSAGSILGALDPDQYEAVPVGITRDGAWVLSNGDPAALSITDRKLPEITTDSGRAVVFPADPTSSGLMVLDPADGVAVLGGVDVVFPALHGAYGEDGTIQGMLEMAGIPYVGANVFASAAGMDKEFTKKLAAAEGIPVGPYAVLRAGCSLSEADKERLGLPVFVKPSRAGSSHGISKVTDWAHLDAAVAKARQIDPKVLVETGIVGREIECGVLEGEAGGAPEASLLAEILVSTDGNDFYDFDAKYLGDGTPYQIPAGLDPEVAQQVRELAARTFTALDCAGLARVDFFVTADNQIYLNEINTMPGFTPTSMFPLMWAATGLEYPKVVDRLIRTALRRGTGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W918</accession>
    <name>U5W918_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31150"/>
    <dbReference type="EnsemblBacteria" id="AGZ44490">
        <property type="protein sequence ID" value="AGZ44490"/>
        <property type="gene ID" value="AFR_31150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6305"/>
    <dbReference type="HOGENOM" id="CLU_893196_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="33047" checksum="8EF6904781D09BBE" modified="2014-01-22" version="1">MAVLVACTAAVIIGANQWRSDEPVAAPAAPVDPGRRPWQVTLDLDTKAGYVISAGVSDGARQGLTVQNLAFSGKPDGVYGGEVTAFPPGTLDEQQLTVGERVNDAWYVPDFTFAEHTARDGKPWQAPAVGRPDPSGVWVVVYADPARSVGEAAIGRDDLLRLAAAVRVGPARDLRLPLSLGAGLPAGLDLTYVRATDQQLDRQPPTLGLSRATREPSGAGLYARPPGGLDVIITTEPRNGDWDKRRPALTGPTTAGGLQAWYEQGKRLTVEAERCVVTIESALTRAELEKLVDRMTIGDCADPESWIPPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W959</accession>
    <name>U5W959_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable transcriptional regulatory protein AFR_31080</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31080</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the TACO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560811.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31080"/>
    <dbReference type="EnsemblBacteria" id="AGZ44476">
        <property type="protein sequence ID" value="AGZ44476"/>
        <property type="gene ID" value="AFR_31080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6291"/>
    <dbReference type="eggNOG" id="COG0217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062974_1_0_11"/>
    <dbReference type="OrthoDB" id="1304328at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="PTHR12532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="SSF75625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01033">
        <property type="entry name" value="TIGR01033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="26612" checksum="040EB46F65100085" modified="2014-01-22" version="1">MSGHSKWATTKHKKAVIDAKRGKMFAKLIKNVEVAARIGGGDPTGNPTLYDAIQKAKKSSVPNDNIANAVKRGSGLEAGGADWQTIMYEGYGPNGVALLIECLTDNRNRAATEVRTALTRNGGTFADAGSVSYLFNRKGVIIVPKSDGVSEDDLMLAVLDAGAEEINDLGEAFEIVSEPTDLVAVRTALQDAGLDYDSAESSLVPSMTVPVDEEAARKVLKLIDVLEDCDDVQNIFANADISDEILAAIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W989</accession>
    <name>U5W989_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_37250</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562039.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37250"/>
    <dbReference type="EnsemblBacteria" id="AGZ45704">
        <property type="protein sequence ID" value="AGZ45704"/>
        <property type="gene ID" value="AFR_37250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7536"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="22318" checksum="6FEEA9A8FAC403A7" modified="2014-01-22" version="1">MDHSAIEVRDLSVSYGSTPVLVDVQLTVPVGAGVCVTGENGIGKSTLLRCISSLQKADAGEIEVFGGPPGSTPEFWRAVVTTVEPPTWYPGLTAREHAELVCRAHGLSPEDAGLDESLERFGIASHADAIPPSLSSGQKQRLTLALALLRPSKLLILDEPEQRLDPDGRAMVAGLLSDYLAGGGTVLMASHDDTFAAAAGTGLVSMADLHAAGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9A3</accession>
    <name>U5W9A3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560859.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31330"/>
    <dbReference type="EnsemblBacteria" id="AGZ44526">
        <property type="protein sequence ID" value="AGZ44526"/>
        <property type="gene ID" value="AFR_31330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6341"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_11"/>
    <dbReference type="OrthoDB" id="1261526at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="335" mass="35802" checksum="E7C055BA6E2675EC" modified="2014-01-22" version="1">MSQVVVIAGASAGIGRAVTAAYAARGAKLALIARGRAGLEGASLEARAAGAADVRTFQVDVSDAEAVQRVADEVAGEWGGIDVWINDAMVSVFAPAWEITPSEYRRVTEVNYLGTVYGTLAALSHMRRAGHGAIVQIGSALAYRGIPLQAPYCASKHAIQGFNDSLRAELLHDGNRVKLSMVQLPAVNTPQFSWVRTRLPRHPQPVPPIYQPEVVARAVLWIADHGKREINVGGPTLKTRLGNIAAPGFLDRLLSSDKRGYQGQQTAEKIDPATWVDNLDKPGDEERDHGAHGVFDDQSRTSSFALWAAMHKPLVTGVVGAGLGLASAALLNRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9H4</accession>
    <name>U5W9H4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Iron complex transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_37090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37090"/>
    <dbReference type="EnsemblBacteria" id="AGZ45672">
        <property type="protein sequence ID" value="AGZ45672"/>
        <property type="gene ID" value="AFR_37090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7504"/>
    <dbReference type="eggNOG" id="COG1119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="18"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="50"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="29687" checksum="8F430B221CDEF831" modified="2014-01-22" version="1">MLEAATSTPTPPDDDTVISFDRVSVIRGGSHLLRGLTWQVELDERWVVLGPNGAGKTTLLNLASGRLHPSRGTAWVLGERLGRTDINELRTRIGLTTGQLAERVPPTERVLDVVVTAAWSVVGRWRESYDPQDEARARALLDQMGMGALVDREFGTLSEGERKRTQIARALMTDPEMMLLDEPTAGLDLGGREDLIGRLTELAEDPDAPAMVLVTHHVEEIPPGFTHALLLREGTVVASGLLGETLTAENLSKTFGLPLVVQRSGDRYSARAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAI9</accession>
    <name>U5WAI9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561298.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33515"/>
    <dbReference type="EnsemblBacteria" id="AGZ44961">
        <property type="protein sequence ID" value="AGZ44961"/>
        <property type="gene ID" value="AFR_33515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6783"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_15_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017205">
        <property type="entry name" value="Sig_transdc_His_kinase_ChrS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037434">
        <property type="entry name" value="STHK_ChrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="2">
        <location>
            <begin position="286"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="375" mass="39513" checksum="AEAFBB4CC8461169" modified="2014-01-22" version="1">MTGPEVARYSISWDLCYAVVALAVATAVAAQSETPSANRLVATGSIMAIALLYAVAGRRADSGPLFVVATLVIFGIGTYAVSTVSYLLFALCPMIFMLLRVRPAVVAVIGANVVPLIAGFLRGGADWYFVTHLGPIFVMSAALAVWLGIWINRVIEQSTERAALIAELESSRAEVARLSHETGVAAERARLAGEIHDTLAQGFTSIITLLQAADPALADERLALAVRTARENLAESRALVAALAPAALGSGSLPDSVRRQATRLTEESGIPATCRVSGDARPLPTAVEVVLLRATQEALTNVRRHAAAHEVAVLLAYGERSVRLVVRDDGRGFDQGAADGFGLPGMRARAEQVNGTLTVRSDPETGTLIEVEVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB72</accession>
    <name>U5WB72_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
            <ecNumber evidence="7">2.7.8.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">UDP-MurNAc-pentapeptide phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">mraY</name>
        <name evidence="8" type="ORF">AFR_34650</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMP</text>
            <dbReference type="Rhea" id="RHEA:28386"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="EC" id="2.7.8.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyltransferase 4 family. MraY subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.13" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561523.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34650"/>
    <dbReference type="EnsemblBacteria" id="AGZ45186">
        <property type="protein sequence ID" value="AGZ45186"/>
        <property type="gene ID" value="AFR_34650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7010"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_0_0_11"/>
    <dbReference type="OrthoDB" id="1151822at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051992">
        <property type="term" value="F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06852">
        <property type="entry name" value="GT_MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00038">
        <property type="entry name" value="MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003524">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00445">
        <property type="entry name" value="mraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="367" mass="38942" checksum="3CE84CA9A49F9F18" modified="2014-01-22" version="1">MRAVIVAAAVAFIVSLFLTPVAIRVFSALKAGQPIRSLGLASNEGKKGTPAMGGVVFIVATLIAYVAGHVALTTLSERQIAQEKPTMTALVLLGVFVFCGAVGFVDDFLKVRKRNSDGLSAKGKLLGQAIIGTGFGIAALYMASTNGQTVASENISFIRDISWLDIGKVGSVILFVFVITAMSNGVNLTDGLDGLATGASTLVLGAYALIGYWQYRHWCADDTYSRVNEYCYQVRDPLEIAMIAAAAAGACVGFLWWNTSPARIFMGDVGSLGLGALIGGLAVATRTTLLSILIGGLFFIITVTWVIQIVSFRTTGRRVFRMVPLHHHFELAGWSEVNIVVRFWIIAGVCVAAGLGVFYSDFLAAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBG9</accession>
    <name>U5WBG9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562669.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40415"/>
    <dbReference type="EnsemblBacteria" id="AGZ46337">
        <property type="protein sequence ID" value="AGZ46337"/>
        <property type="gene ID" value="AFR_40415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8180"/>
    <dbReference type="eggNOG" id="ENOG5033CFU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155914_0_0_11"/>
    <dbReference type="OrthoDB" id="2034873at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="11774" checksum="CC0B80BD11884E28" modified="2014-01-22" version="1">MADEWRAAWITALDELEADVDAVERMITQEHRNQELAEATPWAPPEGIGPIPLDLRPRADYILTRQLEVAQAAAIAMTANRRQTAFAARVEVGTAGKGIPTYIDCAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC37</accession>
    <name>U5WC37_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_36350</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561862.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36350"/>
    <dbReference type="EnsemblBacteria" id="AGZ45526">
        <property type="protein sequence ID" value="AGZ45526"/>
        <property type="gene ID" value="AFR_36350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7356"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_5_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="97"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="18897" checksum="D48DED5E98E7B622" modified="2014-01-22" version="1">MDFDEFYRDTSRRLLRYAYGLTGDPGETQDLVQEAYARAWERWRRVRGYENPEAWLRLVVTRLSSDRWRRIGVRRARAAAAPPLEAMPGPSEDTVLLVRAMRTLPVAHRRALALYYLLDRSVADIAAETGASLGTVKSWLSRGRAGLAAALGEPERTEVPEGVRNAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCW5</accession>
    <name>U5WCW5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">50S ribosomal protein L9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">rplI</name>
        <name evidence="9" type="ORF">AFR_42815</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563148.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42815"/>
    <dbReference type="EnsemblBacteria" id="AGZ46817">
        <property type="protein sequence ID" value="AGZ46817"/>
        <property type="gene ID" value="AFR_42815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8661"/>
    <dbReference type="eggNOG" id="COG0359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078938_5_1_11"/>
    <dbReference type="OrthoDB" id="1959318at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.430.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00503">
        <property type="entry name" value="Ribosomal_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_L9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_L9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020594">
        <property type="entry name" value="Ribosomal_L9_bac/chp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020069">
        <property type="entry name" value="Ribosomal_L9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036791">
        <property type="entry name" value="Ribosomal_L9_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_L9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_L9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="PTHR21368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03948">
        <property type="entry name" value="Ribosomal_L9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55653">
        <property type="entry name" value="SSF55653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="SSF55658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00158">
        <property type="entry name" value="L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00651">
        <property type="entry name" value="RIBOSOMAL_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RIBOSOMAL_L9" evidence="8">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="149" mass="16008" checksum="7CF36F04D6A34D2C" modified="2014-01-22" version="1">MKIILTQEVSGLGTPGDIVEVKNGYGRNYLLPQGFAIQWTKGAEKQVTVIKRARDAREIRDLGQANEVKGQLEGLKVTLTARSGTGGRLFGSITPAEIVDAVKTAGGPVLDRRRLELPTTIKTTGAYKVQVKLHPEVTASFPVNVVAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD02</accession>
    <name>U5WD02_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38205"/>
    <dbReference type="EnsemblBacteria" id="AGZ45895">
        <property type="protein sequence ID" value="AGZ45895"/>
        <property type="gene ID" value="AFR_38205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7729"/>
    <dbReference type="eggNOG" id="ENOG5031XKB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2535083_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666519" evidence="2">
        <location>
            <begin position="34"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="86" mass="9099" checksum="27E1A836F1E42DD2" modified="2014-01-22" version="1">MERMSSASKWFSRLGAAAMGLVLTLVVPVHAWAASNGVADVAVEAARSRRRGGFGFFGILGGLCCLLVVGGIVLAIVLISRNRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-02-10" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDV0</accession>
    <name>U5WDV0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polyhydroxybutyrate depolymerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39730"/>
    <dbReference type="EnsemblBacteria" id="AGZ46200">
        <property type="protein sequence ID" value="AGZ46200"/>
        <property type="gene ID" value="AFR_39730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8038"/>
    <dbReference type="eggNOG" id="COG3509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027551_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010126">
        <property type="entry name" value="Esterase_phb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10503">
        <property type="entry name" value="Esterase_PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666317" evidence="2">
        <location>
            <begin position="28"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="315" mass="32670" checksum="6E48A0FE0977AF55" modified="2014-01-22" version="1">MNGDEMKRLPALVAVVLLALSGCTRNAPQPAPSPSAALPVGKSSAKLAVDGRDRTYLLYRPASARANAPLVIVLHGAVGTGRQAEESYGWDAEADKGGFVVAYPDGVNRTWNASKDCCGVAARDDVDDVGFITQLAGAVPAVDPQRVYVTGISNGALLAYRLACETKIFAAIGPVAGTMINSCDSPSPISIIHIHGKDDPTIPYAGGPGRRSNDGKGRLPARIDGPAVPDLIAEWRRIDSCAAPTEKTAGAVTTSIATCPDGRAVELISVAGAGHQWPGSRPAPIAQKLLKLDPPSDAVAATPVIWTFFAAHPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDX6</accession>
    <name>U5WDX6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative GAF-sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562558.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39855"/>
    <dbReference type="EnsemblBacteria" id="AGZ46225">
        <property type="protein sequence ID" value="AGZ46225"/>
        <property type="gene ID" value="AFR_39855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8064"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_44_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="186"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="407" mass="44186" checksum="AEC106B48444BE6A" modified="2014-01-22" version="1">MPALLPGNEADRLAALYELNVLDSPPEKDFDDIVKLASTVCGVPMSLVSLIDVDRQWFKAKIGTDLTETPRDLSFCSQAILGRDLLVVPDVKEDARFAGHPAVGDGRGVRFYAGAPLITSDGFALGALCVLDSVPRRLDDEQLQALRALARQVTSQLELRRYATALVNTTARLQELERRKDDLAGLVGGKLRAPLRLMRSYLSELGNTGFHNAEMADLVGRATSAHVRGLTDLLDHLSSMADAGHGADSLHMREIDLTRLTQRAVEAVRPIAATKQIWILNQAGGPSLPIIADPVRLEQVLMHLLFAAVKYTPEGGRVRVGTEMESGPTVRLDDMDMPDGMRPDLFPHLYYGAIANPAVVPDPDRGLAVAKRILDAHHATVALSDRPGDGTSLHVVFPTAERELIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEG7</accession>
    <name>U5WEG7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Threonine synthase</fullName>
            <ecNumber evidence="7">4.2.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_40905</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40905"/>
    <dbReference type="EnsemblBacteria" id="AGZ46435">
        <property type="protein sequence ID" value="AGZ46435"/>
        <property type="gene ID" value="AFR_40905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8281"/>
    <dbReference type="eggNOG" id="COG0498">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="6">
        <location>
            <begin position="41"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="383" mass="40001" checksum="9A176DC7C4BF2BD9" modified="2014-01-22" version="1">MAYDAAALASVTREQIEAGPQNIWRYAALLPTGQDPATRVTLDPGLTPLVSATALAAEVGLRAPLWVKDDSANPTHSFKDRVVSVALTAARELGFTRFACASTGNLANSVAAHAARAGVPSIVFIPGDLEQGKVITTAVYGGELVAIDGSYDDVNRLCGELVETDEFEDTAFVNVNVRPFYAEGSKTLGYEVAEQLGWRIPAQVVVPMASGELLTKIDKAFSELVEIGLVEAPEGGWTVFGAQSAGCNPIATALHNGTDQIIPVKPTGIAKSLNIGDPAAGSYAIEAVTRTGGWMDYADDDEIRDAIQLLARTTGVFAETAGGVTVAVLKKLVASGKLDPDKETVVYNTGEGLKTLDAVAGQVGPTHRIKPSLRGARDAGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFW1</accession>
    <name>U5WFW1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">6-O-methylguanine-DNA methyltransferase</fullName>
            <shortName evidence="7">MGMT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_42885</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 7">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MGMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563161.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42885"/>
    <dbReference type="EnsemblBacteria" id="AGZ46831">
        <property type="protein sequence ID" value="AGZ46831"/>
        <property type="gene ID" value="AFR_42885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8676"/>
    <dbReference type="eggNOG" id="COG0350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_11"/>
    <dbReference type="OrthoDB" id="1327994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA dealkylation involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00772">
        <property type="entry name" value="OGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023546">
        <property type="entry name" value="MGMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="SSF53155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_binding_1" evidence="8">
        <location>
            <begin position="64"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; methyl group acceptor" evidence="7">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="15872" checksum="84CBE716C9024369" modified="2014-01-22" version="1">MQLLVESPIGPLGVRLDGDVVVGVRFGATTGETGDHPAVQQLEAYFAGELTDFTVPVELPGGSDFERSVWGQIAQIPYGEMLTYGAIAIALGDPGAARAVGTACNHNPVPVIVPCHRVVGAGGKLTGFGGGLPRKRQLLELEARVALQRAWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFX6</accession>
    <name>U5WFX6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Membrane protein insertase YidC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Foldase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Membrane integrase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Membrane protein YidC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AFR_42985</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the Sec translocase complex via SecD. Specifically interacts with transmembrane segments of nascent integral membrane proteins during membrane integration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXA1/ALB3/YidC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563181.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42985"/>
    <dbReference type="EnsemblBacteria" id="AGZ46851">
        <property type="protein sequence ID" value="AGZ46851"/>
        <property type="gene ID" value="AFR_42985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8698"/>
    <dbReference type="eggNOG" id="COG0706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036138_3_1_11"/>
    <dbReference type="OrthoDB" id="524437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="265"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AGZ46851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="346" mass="38139" checksum="784D8082BBC35FC9" modified="2014-01-22" version="1">MSLDWIYYAISWILLRWHDLWDTIGIPDGPVLGTNWSWILSIVFLVVTLRVILFPVFVKQIKSQRAMQALQPKVKLLQEKHKGDRETLQKEMMELYKVEKANPLMGCLPMFLQIPVFLGLFHVLRRLSPTNSQQTLYGWTVEQFDSATHASLFTAPLASKFGSSATELQLLGANGVTVKIIAGILVLTMMATTYLTSRQMILKTGWAEDPQQKMIQRLMLYGIPLSLLVSGSLFPIGVVIYWVTNNLFSLGQQQWVLRKFPPPPMANAKAGSTPARSNGSNGTKPGAKGPQSPVRSGRTGGLFGRKAAAEPEPKSPVVDTKALAPKPGAKPVNPKKGARPANKPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNU7</accession>
    <name>U5VNU7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hydroxyacid oxidase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_01595</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01595"/>
    <dbReference type="EnsemblBacteria" id="AGZ38608">
        <property type="protein sequence ID" value="AGZ38608"/>
        <property type="gene ID" value="AFR_01595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.321"/>
    <dbReference type="eggNOG" id="COG1304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="63"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="268"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="33396" checksum="64970F31D8B29CF9" modified="2014-01-22" version="1">MALPAAVYAYYRDGAGEQISRDEATAAWRDWRFVPRLFRDVSRISTELELFGARLAAPVLVGPTALHTLAHPEGEAATARGVEAAGSLFVLSSRSGRPLEEIPLTRPWWFQAYVLRDRSITEQNVRRAVAAGARAIVLTADAPYVLKRAVPEAGELGPANRPPGLEQDPALGPDAISWLAEISGRPVVVKGVLRPEDAVRCVEAGAAGVIVSNHGGRQLDRALSTARALGPVAEAVGGRVPVLVDGGIHNGADVLTALALGATAVLIGRPVLWALATGGAEGVRECLDFYGRELSQVMAQAGLATLDEVDSSAVTPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNW3</accession>
    <name>U5VNW3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_00975</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357427.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00975"/>
    <dbReference type="EnsemblBacteria" id="AGZ38484">
        <property type="protein sequence ID" value="AGZ38484"/>
        <property type="gene ID" value="AFR_00975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.195"/>
    <dbReference type="eggNOG" id="COG1295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045539_3_0_11"/>
    <dbReference type="OrthoDB" id="1421045at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017039">
        <property type="entry name" value="Virul_fac_BrkB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30213">
        <property type="entry name" value="PTHR30213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03631">
        <property type="entry name" value="Virul_fac_BrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00765">
        <property type="entry name" value="yihY_not_rbn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="354" mass="37563" checksum="7E1D09D89AC31BE7" modified="2014-01-22" version="1">MSSDQKSGRGDNIDLDAVATTRTGDDRPNDLSAPSPDAGPDSPAQLKGKGLFGALKRTVKQFSQDNITDWAAALTYYGVLSIFPGLLVIVSLLGMLSSNGQQTVQDAVRELAPNPQLQNLVGTVLDQVKDPGTAGLAAIIGIVAAFWSASGYTAAFMRASNAVYDVPEGRPIWKTLPIRVGVTAVVGLMLIISAAIVIFTGDLAEIVGDKIGLGSAAVLTWNIAKWPVLLMLVSLMFAILYWASPNAKTGAFRWVSPGGVFAVVLWILASAAFALYLANFANYNKTYGTVGGVIAFLVWMWITNIAILLGAELDAELQRGRAIAAGHDPTDEPFLELRDDRKIKPGSEQGLSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPG2</accession>
    <name>U5VPG2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357801.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02845"/>
    <dbReference type="EnsemblBacteria" id="AGZ38858">
        <property type="protein sequence ID" value="AGZ38858"/>
        <property type="gene ID" value="AFR_02845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.571"/>
    <dbReference type="HOGENOM" id="CLU_798387_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025326">
        <property type="entry name" value="DUF4232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14016">
        <property type="entry name" value="DUF4232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="347" mass="37042" checksum="B694E3D9CBF1ECDE" modified="2014-01-22" version="1">MDLETDLRTALGDSRHELVPPPHATETIVRNVCRRRRRRTVTWAAAGLIVVAVGVPVALRVSPSDSTVVTADGTVGWVAEPLAQAPQLARRSPRPAAPACDPAKLNPTIGYEDVLAADGTMLRSVVVTNRTDRRCTLSGIPTVKGTLTSTGARTTVVAYPVADEPGELRQYPATIDPGEAARVDVTKGSAGCDATELTDVVLEFGARSFPASPKSDEVMCAPRAGSWYVRPPLLNAPVMAKISAPAQVRRGETFDYTVELTNGYNRTFSYGGCPAYRQEFAGSKGWFRLNCGPRKIGPHSSVTYAMRATVPKDAEKGGRLTWMAAMPDGEVVIANLDTGGVPVEIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPU8</accession>
    <name>U5VPU8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YrdC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357926.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03470"/>
    <dbReference type="EnsemblBacteria" id="AGZ38983">
        <property type="protein sequence ID" value="AGZ38983"/>
        <property type="gene ID" value="AFR_03470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.697"/>
    <dbReference type="eggNOG" id="COG0009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_3_0_11"/>
    <dbReference type="OrthoDB" id="1913202at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YrdC-like" evidence="1">
        <location>
            <begin position="14"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="22596" checksum="F37BEF1BEBC62001" modified="2014-01-22" version="1">MAKYFDVHPDNPQTRAISQVVGILRDDGLIAYPTDSCYALGCRIGNAKGLDRIREIRRLGSGHHFTLVCRDFAQLGQFVQINNAVFRLVKASTPGSYTFILPATREVPRRLLHPKKKTVGVRIPENVVTQAILAELGEPLLSSTLLLPGQEEPLTQGWEIKEELDHVVDAVIDSGECGTVPTTVVDLSDGEPEILRVGAGDPSRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQE4</accession>
    <name>U5VQE4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03700"/>
    <dbReference type="EnsemblBacteria" id="AGZ39029">
        <property type="protein sequence ID" value="AGZ39029"/>
        <property type="gene ID" value="AFR_03700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.746"/>
    <dbReference type="eggNOG" id="COG3209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003050_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="964" mass="101857" checksum="497F8E8A89E86CEF" modified="2014-01-22" version="1">MAEARSTGKDVLVDKATSGTSLTWARPDGQMRTQVTAVPQRAKNSAGQWAPIDLKLTRTGNTVQPGNSPVPVRFSAGSKTPGDTTLAEMDLDGRTITYTWPGALPEPVLDGSRALYPEVLSGVDLLLVAREEGGFANLLIVKNREAAANKELGSLAYGLSSAQAVFRHDSTTGGVRIFDAKTKAEIGSVPTPFAWDSSGRDPESPTAPRLSVATPADVLELSGLQGLEPGVRQAPMPTRLDGDGTGKAVLHLDAAGTGLLTDKNVRFPVFLDPPINSGEQAWTLVHKPNPNSNFLNGTGFNGGTSEARVGYETETNGLARSFWRMAFKNIKGATVSSASFKVLNTHSWSCTKREFQLWHTGAISSGTTWNKQPSWITLQQKVSFAYGYASSCSDDYAAFNIKNAAQAAADSGSTNLTLGMRATSEGDTETWRKFKANSATLEATYNTPPKEPTGGTTNPGGACTPGPGNGVTIAKTNIVLSATATDPDGNLEKLRFRYWKTGGTVPSGVLVTPNSSGKASTTIASTTLVDKATYSWDVRSEDSSDAVSSFFPGNTTEPCRFTVDASAPAAPEVTSDDYPEATSDGATWATKKFGETGSVTFTAEGAARFEYSFEYISPLSVTANASGTATVTGLKPRHAGPTSLIVYAYDKAGNRSVLTSYTTYVPPRDDADGPGDTGGDGINDLLVVDGNGHLRTLPGDLAGELYTSLASSYTTGNKLNPAGHWFDSATGKSALIAHYDDAYPGDGITDLFARTPDGGFYLYPGDGYGSFNVDQRLRVLLPSNTPAPSTWTELLAVGDITGDKRPDLALRAGTAFWVLSGYSGASFQEAVLMEGTAWARRDLIAIRDIDVDGTPDLLWRNLDAGGMYVRHGKPGTIAGSVSLDSIKIAGNSRAGDVSYGTNWQETAIKEVISFPDSNADGVADLWAISATDGNTRLYNPSKTNTGASIKTVLSNDWRSIKAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQT7</accession>
    <name>U5VQT7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rpsH</name>
        <name evidence="7" type="ORF">AFR_04575</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit. Contacts proteins S5 and S12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358193.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04575"/>
    <dbReference type="EnsemblBacteria" id="AGZ39204">
        <property type="protein sequence ID" value="AGZ39204"/>
        <property type="gene ID" value="AFR_04575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.924"/>
    <dbReference type="eggNOG" id="COG0096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_0_1_11"/>
    <dbReference type="OrthoDB" id="1692188at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_B">
        <property type="entry name" value="Ribosomal_S8_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="135" mass="14816" checksum="3758C582C47B2772" modified="2014-01-22" version="1">MTMTDPIADMLTRLRNANQAYHDKVTMPFSKIKANIAEVLKAEGYIASWTAEDPAEGEVGKRLVVELKYGQSRERSLAGIKRVSKPGLRVYAKSDELPRVLGGLGVAIVSTSQGLLTDRQARKRSVGGEILAFVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRD2</accession>
    <name>U5VRD2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358945.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05625"/>
    <dbReference type="EnsemblBacteria" id="AGZ39414">
        <property type="protein sequence ID" value="AGZ39414"/>
        <property type="gene ID" value="AFR_05625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1141"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076053_1_0_11"/>
    <dbReference type="OrthoDB" id="1389630at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017523">
        <property type="entry name" value="CHP03089"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03089">
        <property type="entry name" value="TIGR03089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="25278" checksum="9619F15E760E8F0D" modified="2014-01-22" version="1">MIETIPAALAAAVRRDPTTPLLTWYDDASGDRTELSGATMANWVAKTANLLVDGVGLGSGDSVAVLMPPHWQTAAVLLGSWAAGLAADLGPDPRPVEVLFTTPERAEVAAGWSTTDRYATGLLPLAAPLRQIPPGFADYTVEVRAFGDYFSSSPVDKNDRALAGPVEESHLSVCEMATERAVELGIKPGDRVLVDASAYPDPTDWLLAPLIAGASIVLCGNLDAAKVQSRTEAEKATLLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRP1</accession>
    <name>U5VRP1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359060.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39529">
        <property type="protein sequence ID" value="AGZ39529"/>
        <property type="gene ID" value="AFR_06200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1259"/>
    <dbReference type="HOGENOM" id="CLU_1048184_0_0_11"/>
    <dbReference type="OrthoDB" id="1846523at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="28634" checksum="66C9AA37DAD23713" modified="2014-01-22" version="1">MTQFYPQDPLVSGDFNGWWRRSVWLIRAGWRPMALVQVVVTVPIAAIVIPAFSRFEKEQAEAHRIMETTDLTPGLIFAGWTSLVPVIVIGSLLYLLGQLAVAQLVISLATGRGTALGPAVATAARRTPALLGWFLLAVPVLLISLALCFFPAIYVGAVITILPVVVLVERRRGIGRCFQLFNADLGLSIARIATIIGLTFAASLALTLVTTLVDVVAPNLIGTSLKVLLETSYYIVSGVVVTTMVVTAYADMRSRHERFSSADLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRU4</accession>
    <name>U5VRU4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00855"/>
    <dbReference type="EnsemblBacteria" id="AGZ38460">
        <property type="protein sequence ID" value="AGZ38460"/>
        <property type="gene ID" value="AFR_00855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.171"/>
    <dbReference type="eggNOG" id="COG3795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130902_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="5"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="127" mass="14161" checksum="3C508864EC23E62C" modified="2014-01-22" version="1">MEAPMKYLILIQSNERSLAVWETLSEQQRFEFGRGHLQLSEEMAKAGVLVSSEGLGDPSLARWVSVRDGKTVASDGPFAEVKEHLAGFYLIDVESLDEAIGWAAKVPDAAYTEVEVRPVLDMSEWEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSV3</accession>
    <name>U5VSV3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08300"/>
    <dbReference type="EnsemblBacteria" id="AGZ39949">
        <property type="protein sequence ID" value="AGZ39949"/>
        <property type="gene ID" value="AFR_08300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08300"/>
    <dbReference type="eggNOG" id="ENOG5033JFH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168298_2_0_11"/>
    <dbReference type="OrthoDB" id="2182238at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="101" mass="11277" checksum="4DE74815B394309E" modified="2014-01-22" version="1">MGIVIPTDEVRKHAREVDEVSRMLDEARGAVSFIRASSNAYGYPVGPLFTSAYLNPHRDEAIASYRRAVVGMRPLADLLRAMANDFDHSDECPAERLRGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSY3</accession>
    <name>U5VSY3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase GCN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359594.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08870"/>
    <dbReference type="EnsemblBacteria" id="AGZ40063">
        <property type="protein sequence ID" value="AGZ40063"/>
        <property type="gene ID" value="AFR_08870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1805"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_11_6_11"/>
    <dbReference type="OrthoDB" id="1823496at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="18302" checksum="D29CA02B122DBFAA" modified="2014-01-22" version="1">MITIRTVAADAPDAVALVRSYLGEMVDRYHGRPMPGSAVDAALADEPADDVAVLLVAYRMIADRAVSGQTVSDRGGEPVGCVGLRPAEAGTGEITKMYVRPEARRLGIGRRLLSAVEDEARARDLHTLRLDTRNDLTEARAMYAAAGYVEIPPHRDRLYADHWFAKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTP9</accession>
    <name>U5VTP9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358010.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03880"/>
    <dbReference type="EnsemblBacteria" id="AGZ39065">
        <property type="protein sequence ID" value="AGZ39065"/>
        <property type="gene ID" value="AFR_03880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.784"/>
    <dbReference type="eggNOG" id="COG0402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_3_11"/>
    <dbReference type="OrthoDB" id="1592010at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="56"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="476" mass="51932" checksum="BFE21DB5DB1F7880" modified="2014-01-22" version="1">MSDSVLFRNGTVLTMDDRHTVLPNADVLVIGERIAEIGVGLTAPEGATVIDASGGILMPGMIDTHRHMWQTAMRGYGADWTLTQYFVWYYLESGKLFRPEDIHAGNLLAAIEAIDAGVTTTVDWSHGLQTVDHADAAVDALQAVPGRFVLAYGNIQQGPWEWSASPEFRDFVSRRITPGNDMLGFQMAFDVTGDPAFPEKAAFEVARELGVPVTTHAGVWGATNDDGIRLMHENGFMTPQTVYVHSATLNHDSYHRIAATGGSVSVSTESEQSAGQGYPPTWTLRDHGIPVSLSMDTSVWWSGDLFSAMRTTLGADRSREHMEAHSRAETVTHLHLRAEQVVDWATRGGSKALGMDSVVGSLEAGKKADVVLLKNDDSPVMFPILNPYGHVAFQAQRGDVDTVLVNGKIVKRDHKLVGVDLAAARAEVGRTIDHLRGEMGEEAWTRGMNPDVPETKVGDNPYTYTEWDAGSAQWKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU44</accession>
    <name>U5VU44_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_10215</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360106.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10215"/>
    <dbReference type="EnsemblBacteria" id="AGZ40332">
        <property type="protein sequence ID" value="AGZ40332"/>
        <property type="gene ID" value="AFR_10215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2082"/>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_33_11"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="163"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="223"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="196"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="424" mass="43768" checksum="6EE3FAB53470C224" modified="2014-01-22" version="1">MRRRLTLLVAATTCLVLIAFLVPLALLIRQVTEDRAISRANDVLQTIVPQVGTSDDAALALVVDGLPLPVSVFPPSGAVLGAAAERTPAVRLAQANGRSFTVSSDAGREIVVPVSGTDGTYVVRTVVPADRLTSGVVRSWLVLAGLGLALVLLGLVVADRLARNLIAPIADLSAVSHRLARAELSARASPSGPPEVREVATALNQLAGRIQDLLREEREQVADLSHRLRTPLTALRLEAESLRDEEEAARVTAAADGIERAVTAVIQQARRRAGERPATADATAVVTDRVAFWTVLAEDTDRQVRRELPSAPLPVALPADDLAAALDAVLGNVFAHTPDGTALAVSLVPRAGGGAVLTVSDAGPGFPTGALRRGSSGSGSTGLGLDIARRAAEAAGGALFLESAPGGGAQVRLELAAVKQLPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUD9</accession>
    <name>U5VUD9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lysine exporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_10740</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360313.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10740"/>
    <dbReference type="EnsemblBacteria" id="AGZ40437">
        <property type="protein sequence ID" value="AGZ40437"/>
        <property type="gene ID" value="AFR_10740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2189"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087840_2_0_11"/>
    <dbReference type="OrthoDB" id="1953934at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="19247" checksum="25AAE7EE5A1331C9" modified="2014-01-22" version="1">MTGPFVAGVIAGYGVAVPVGAIAVLIAGLTARTSLRVGAAAGLGAATADGIYALLAVLGGAALAGAVAPIATPLRWAAAGVLLLLAAHTAWSALRKPAVAASTDTTTAGAAYLGVLVLTLLNPMTIVYFAALVLGRGGAGGGVWFVLGAFLASASWQLLIAGGGSLIGQILTGPRGRRTTALISSAVITILAISLITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUX8</accession>
    <name>U5VUX8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_12560</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360857.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12560"/>
    <dbReference type="EnsemblBacteria" id="AGZ40798">
        <property type="protein sequence ID" value="AGZ40798"/>
        <property type="gene ID" value="AFR_12560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2551"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_4_1_11"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="9">
        <location>
            <begin position="6"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="25969" checksum="B5B0AC8281C6E4CF" modified="2014-01-22" version="1">MSASAAVLKAETKLFAREPGSLFWVLAFPSLLLLGFGLIPKYREIDEGLGGNRIIDFYVPSVVLVALVTASLLSLPGTLTGYRERGILRRMRTTPARLADLLIAQMALHAVAAILGGVLAIAVGRLVWGVALPEQPLAYGVTLLLATLAGLATGSVITALARTAKAAQAVGLAALFPAMFAAGIYVPIQVLPDVVRQIIALLPFGAAAQALNQAASGSWPAWSHLAVLVLWTVVPMVVAARWFRWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV90</accession>
    <name>U5VV90_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12180"/>
    <dbReference type="EnsemblBacteria" id="AGZ40722">
        <property type="protein sequence ID" value="AGZ40722"/>
        <property type="gene ID" value="AFR_12180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2476"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032783">
        <property type="entry name" value="AraC_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12852">
        <property type="entry name" value="Cupin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="221"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="35738" checksum="9615BE921BDBC8ED" modified="2014-01-22" version="1">MESAISYVSSKSRSPLGSSDPIAAAIGLLRPRTAVDPDFSAAGPWALRFEAFEYVKIGGIEQGECWLVFEDDSDPVRLRKGDVFLLVNPPAYRLASSPTVEPLPAQALWESAVKGRVCIGTEADEDFSMCGGQFWFDQANTTMLIDVLPRLVHVRAGDRSLGQLAQVAHLLSTETKNRTVGSSLVLDHLVQILFVHVLRAHVDQTDHPAGWLGALSDDGVGAALRALHADISHRWSLQELAGIARMSRSAFAAAFKKQLGTTPGEYLIQWRMSLARDALRRNTRSISELAVATGYESESAFSTAFRRVVGSSPKQYRDAPGERMTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVH5</accession>
    <name>U5VVH5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359241.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07105"/>
    <dbReference type="EnsemblBacteria" id="AGZ39710">
        <property type="protein sequence ID" value="AGZ39710"/>
        <property type="gene ID" value="AFR_07105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1450"/>
    <dbReference type="eggNOG" id="ENOG5031MWT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111071_0_0_11"/>
    <dbReference type="OrthoDB" id="1992831at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010773">
        <property type="entry name" value="Mycophage_PG1_Gp7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07098">
        <property type="entry name" value="DUF1360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="19815" checksum="435DCFDBC7F98B6C" modified="2014-01-22" version="1">MFPSVRGRLNRLRRAYAPHEHRPLDGYLVTMGAFGVLAGTLATAAKLSGRPVPERPAAADVVLISIATHKLSRLIAKDSITSPLRAPFTRYAEPGGSAEVNEEVRDEGSTLRHSIGELVSCPFCLAVWVATGLTSGLVFAPRLTRLAATVFTATAASDFLQMAYSLAKEAAEGEPQEDEQPALAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVW7</accession>
    <name>U5VVW7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13330"/>
    <dbReference type="EnsemblBacteria" id="AGZ40952">
        <property type="protein sequence ID" value="AGZ40952"/>
        <property type="gene ID" value="AFR_13330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2706"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057459_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="38105" checksum="E8A19E927E37D1EA" modified="2014-01-22" version="1">MVLTAAGASVAGVWAWRRDTTAAPAAAAPSTATAEIVRQDLSTSVSVTGKLGYGVARSVKGSRPGIVTWLPSPGATIRRGSALYRVDDEPVPVFYGRIPLYRRLEKLNTVGRDVRVVADNLEALGYAVGRRYRPGDHVRQTSPVPLVPEGGAPSAPPASRTSWVKVGKGEDVLTTALVRAVGRWQNDIGLPASGSLGIGDVAVLGGAVRVESTAVQVGDSAEAALLAVTPTVQVVTIEAEPAEANGMKRGEKVQVHLPDDRTVPGRVSRVATAVTTDPDTPPKLTVTVTLDDARKTVRLDAAPVEVVLPGRSRNDVLVTPVGALVALAEGGYAVQVAGGGLVAVETGMFAGGLVEVSGAGLSEGTLVVTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVZ2</accession>
    <name>U5VVZ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_13740</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361093.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13740"/>
    <dbReference type="EnsemblBacteria" id="AGZ41034">
        <property type="protein sequence ID" value="AGZ41034"/>
        <property type="gene ID" value="AFR_13740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2790"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="4">
        <location>
            <begin position="189"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="338"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="42659" checksum="D96A66CF9857853F" modified="2014-01-22" version="1">MLGVPALPLWTSRLWLLFCAVLITVAVPVTVADYDIGLPLAFAVCSVQAGSLALAVNWPRLGAGLHLVATAVVVLVNRDSPDQPWPLPITGLVSLGALLLLLGVRERWTVSVSVWWLSILMLVALVATSPDRYADPDQWGVNLTIYSSYTVTLLAAAIAVGQRGHIRRDLAQARRDVELEQAQRRYVEERARIARELHDVVAHSMSLVHMKALSAPFRLTGPRPADTDAEFNDIARLARAALTEMRQLLRALRSDEDASDPVPQPQVADLHELAAALVKAGTVIDLSVDERAGNTSPIVQLTIYRVVQEALSNVVRHAPAARTRVAVQVSDSQLVVSVHNEPSPRGGFAPVATTRPDRGGHGLRGMRERVSLLGGELSTGPTPDGGFAVEATIPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW40</accession>
    <name>U5VW40_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359407.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07935"/>
    <dbReference type="EnsemblBacteria" id="AGZ39876">
        <property type="protein sequence ID" value="AGZ39876"/>
        <property type="gene ID" value="AFR_07935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1620"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_4_0_11"/>
    <dbReference type="OrthoDB" id="949956at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102483">
        <property type="term" value="F:scopolin beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="SSF52279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="2">
        <location>
            <begin position="362"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="790" mass="83345" checksum="69E96F9067CCB01D" modified="2014-01-22" version="1">MDDLLARLTLEEKVSLLGGQDFWSLPAIPRIGLRSLVMSDGPIGVRGTGWAPEDPSIALPSPTALAATWDPSLARTAGRLLGQESRRKGVHLLLGPTVNLQRTPLGGRHFECYSEDPLLSGEIGVGFVEGVQDHGVGTTVKHLVGNDFETERMTVDVRIGERALREIYLAPFERVVAAGGWGVMSAYNGVNGSPMASNGRLQSDILKDEWGFDGIVVSDWRAARSTVGAALGGLDIAMPALENPWGDKLVVAVRAGEVPEEVVDDKVRRVLLLAARAGLLDGVPGAVLTPPADLDGDAVAHEVAARSFVLARNENFTLPLEAAALTRLALIGALATDARVLGGGSAQVTPPHVISPLAGLGRALPGVDVAYAVGADPRPFLPVLPLPASVELLGADFEFSVDTAAVRWIGDLPGAVDPAVVTGLELRTTFTPDEDGEHTFAVSGFGTFDLRVGTVEVPVGTLHPPTATRADLLLSPREHRAVVPLTAGEPVEIVLRQTIKPGMAHTVSATLGHHAPGVSAESMLDEAVALAAECDVAVVVVGTTEQTESEGFDRKSLALPGRQDELVARVAAANPRTIVVVNAGSPVLLPWADDVAAVLLTWFPGQEAGAALADVLLGVLEPGGRLPTTWPRREQDCPILEVVPEDGVVEYDEGVFIGYRAWERAGTTPLYAFGHGLGYTTWEYETLAATATEAVVTLTNTGAREGREIVQVYVAPQDPPAAAALERPARWLAGFEAVSARAGETVTVRIPLPPRTWQVWDEGWHTVPGTYAVTAAHALDDPRLTAGLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW96</accession>
    <name>U5VW96_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavin-dependent monooxygenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">TetX monooxygenase</fullName>
            <shortName evidence="1">TetX</shortName>
            <ecNumber evidence="1">1.14.13.-</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An FAD-requiring monooxygenase active on some tetracycline antibiotic derivatives, which leads to their inactivation. Hydroxylates carbon 11a of tetracycline and some analogs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a tetracycline + H(+) + NADPH + O2 = an 11a-hydroxytetracycline + H2O + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61444"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:144644"/>
            <dbReference type="ChEBI" id="CHEBI:144645"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of an N-terminal FAD-binding domain with a Rossman fold and a C-terminal substrate-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aromatic-ring hydroxylase family. TetX subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359541.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08605"/>
    <dbReference type="EnsemblBacteria" id="AGZ40010">
        <property type="protein sequence ID" value="AGZ40010"/>
        <property type="gene ID" value="AFR_08605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1751"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_4_0_11"/>
    <dbReference type="OrthoDB" id="504558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00845">
        <property type="entry name" value="TetX_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043683">
        <property type="entry name" value="TetX_monooxygenase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1 3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="2">
        <location>
            <begin position="3"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="360" mass="37910" checksum="F9F3EBAA0EC837D8" modified="2014-01-22" version="1">MRIAIVGGGLGGLTLARVLHRHGLEAMVYEREASRSARSQGGTLDLHPESGQHALAEAGLTARFRSQARPEGEEHRILDPAGRMLVHHRPEPGTFTGRPEIDRSALRDLLLDALPGGAVAWGHQVVAATPRASGGWELTFEGGRRAGCDLLVGADGARSVVRSLLTGTAPSPVATLVELIVSDVDRRHPELARLVGPGNLWCVGVNQILAAQRAGDGSLRVGISLPAEDHRELSELFEGWDPGVTALVTAADGPATVRRIEALPIGARWDHRPGITLVGDAAHLMPPVGEGANQAMLDGAELANHLAAGIADPDAAVRAYEEGMFARIGPVAEMSARVQAMMLSPTAAEDIVRFFTPATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX78</accession>
    <name>U5VX78_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Error-prone DNA polymerase</fullName>
            <ecNumber evidence="3 12">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">dnaE2</name>
        <name evidence="14" type="ORF">AFR_09885</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase type-C family. DnaE2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3 12"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09885"/>
    <dbReference type="EnsemblBacteria" id="AGZ40266">
        <property type="protein sequence ID" value="AGZ40266"/>
        <property type="gene ID" value="AFR_09885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2016"/>
    <dbReference type="eggNOG" id="COG0587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001600_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01902">
        <property type="entry name" value="DNApol_error_prone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011708">
        <property type="entry name" value="DNA_pol3_alpha_NTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040982">
        <property type="entry name" value="DNA_pol3_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023073">
        <property type="entry name" value="DNA_pol_error_prone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029460">
        <property type="entry name" value="DNAPol_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004805">
        <property type="entry name" value="PolC_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32294">
        <property type="entry name" value="PTHR32294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32294:SF4">
        <property type="entry name" value="PTHR32294:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07733">
        <property type="entry name" value="DNA_pol3_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17657">
        <property type="entry name" value="DNA_pol3_finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14579">
        <property type="entry name" value="HHH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00594">
        <property type="entry name" value="polc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6 12 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="POLIIIAc" evidence="13">
        <location>
            <begin position="56"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ40266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1106" mass="121087" checksum="E65EE5E27901AD10" modified="2014-01-22" version="1">MPWSALEGTLSGKKHLHVVDPLAVDGDGGDSPAWSRKRQPYQAPAFIRAPGADKYAELHCHTNFSFLDGASHPEELAEEAARLGLTGLAVTDHDGFYGVVRFSQAARELGLPTIFGAELSLDLSRPQNGEADPEGRHLLALAHGPEGYARLARTISRGQLDGAEKGKPVYGDLEDVAAVLRDHVLVLTGCRKGSVPRALAEGGMPAAAYELDRLVALFGRSNVVVELTDHGDPYDDDRNDALYELALSRQLEVVATNNVHYATPARRKLATALAAVRARRSLDDMDGWLPAAGTAHLRSAAEMKRRFAGYPGAVENAAMFGEDLAFDLNLVAPKLPDFPIPEIGHTEMSWLRELTMRGAQERYGPPQAHPRAYAQLEHELKMIDELNFPGYFLVVYDIVKFCRDEGIYCQGRGSAANSAVCYALRITNVDAVEYDLLFERFLAPERDGPPDIDVDIESDRREEVIQHVYGKYGREHTAQVANVITYRPRSAVRDMAKAFGFSAGQQDAWSKQIDRWGSVATVDVEDIPEQVVAYANEVQSFPRHLGIHSGGMVICDRPIIEVCPVEWGRMPGRTVLQWDKDDCAAIELVKFDLLGLGMLSALHYAYDMIESDLDISTMRLDDPEVYAMLCRADSVGVFQVESRAQMATLPRLKPDKFYDLVVEVALIRPGPIQGGSVHPYIRRKNGLEKPEVPHPLMANALAKTLGVPLFQEQLMQLAIDVAGFDPSEADQLRRAMGSKRSVEKMERIKIRLYAGMAERGITGQLADDLFVKLSAFANYGFPESHAMSFAYLVYASAWLKRYHPAAFCAALLNAQPMGFYSPQSLVDDARRHGVEVRRPDINLSDAAAVLESTAQTRWGSAADEPPHAWGLGGPAVRMGLSSVRTLGDDLAKKIEDERRAHGAYRDMADLARRTGCSTAHLEALATADAFAGFGLSRREALWAAGAAAQDKPDRLPGTVTGTEAPTLPGMADVDKLVADVWATGLSPDAHPAQFIRGEFERTGALPISRLGRVEAGTRIRVGGIVTHRQRPATAGGVTFINLEDETGMLNVTCSPGLWQRYRKVARTSAALIVRGRLEKTEGVLNLVADRLEAVTPPISPASRDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXC4</accession>
    <name>U5VXC4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361690.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16650"/>
    <dbReference type="EnsemblBacteria" id="AGZ41608">
        <property type="protein sequence ID" value="AGZ41608"/>
        <property type="gene ID" value="AFR_16650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3376"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_10_1_11"/>
    <dbReference type="OrthoDB" id="1942521at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665844" evidence="1">
        <location>
            <begin position="32"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="62"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="332" mass="34217" checksum="18AF7A852DDC6D65" modified="2014-01-22" version="1">MRITLPRRTLVAAAAVSALLLAACGTTENPADTAGPSTGVSSGPVDLTDERGKVHLDAPAKAVVSLEWGLTEGLVALGVKPVGQADVKGYNNYDTVAPIDASTPDVGTRGEPSVDAIVALKPDLVVTTTDLPENVIASLAGQVPVIALRGSDGTDPIGYMKRTVQTLAQATGTQAQGEKLIADFDAKITAGKDALAKAGKKNAPFTMSDGWIDSGTVSVRMYTPGSFFGGVAAELGLENQWTTGGDKDYGLAQTDIEGLTKITNPETTFLYVANDADGGPAFVKALTGNAVWKQLPFTKAENLHRIPDGIWMFGGPPSAGAYVDAVVAAFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXE3</accession>
    <name>U5VXE3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative gluconeogenesis factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_10555</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for morphogenesis under gluconeogenic growth conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gluconeogenesis factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10555"/>
    <dbReference type="EnsemblBacteria" id="AGZ40400">
        <property type="protein sequence ID" value="AGZ40400"/>
        <property type="gene ID" value="AFR_10555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2152"/>
    <dbReference type="eggNOG" id="COG0391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044041_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00973">
        <property type="entry name" value="Gluconeogen_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010119">
        <property type="entry name" value="Gluconeogen_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30135">
        <property type="entry name" value="PTHR30135"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="SSF142338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="486" mass="49216" checksum="2C108041448093FE" modified="2014-01-22" version="1">MTAAQPGVPGGHPAEPRGDTARHETTTEQPLGHAGPTGEPLANAGLTGEPLVNAGLTGEPPANAGLTGEPLANDGPIGEPPANDGLTAGQTAGDRATTLPSAAGRDADDRSDTRLPGAGLTAGGRGKTRPIKVVAFGGGHGLAVSLRGLLRCAPELDLDITAVVTVGDNGGSSGRLRAERDAQLPPGDLRMALAALADDDPGHQRTVALLQHRFALVGKPDRRAADGIPVSSGESFVQPAGAEVPGDRRIASVGGKHDPLAGHAVGNLLLLGLMELLGDPVEALEHAGAMVGARGRVLPMACHAVGIEADVRGGDPARPDDVVTVRGQHAVAVTTGHVEALRIVPASPPACPPAVAAIAEADWLIFGPGSWYTSVLPHLLVPELAAAIVASPARRVVTLNLAADNETLGMSVADHLAALHWYLPQLRVDAVLADAKWVGEPDPVHRAAEALGARLVLASLAVADGSPKHDPESLGTALVPVLGSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXQ1</accession>
    <name>U5VXQ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_015620263.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10785"/>
    <dbReference type="EnsemblBacteria" id="AGZ40446">
        <property type="protein sequence ID" value="AGZ40446"/>
        <property type="gene ID" value="AFR_10785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2198"/>
    <dbReference type="eggNOG" id="ENOG5032ZDA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161414_1_1_11"/>
    <dbReference type="OrthoDB" id="2057624at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045745">
        <property type="entry name" value="HTH_58_Actinobacteria-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19575">
        <property type="entry name" value="HTH_58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_58" evidence="1">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="75" mass="8150" checksum="8371DFDF0AE0897E" modified="2014-01-22" version="1">MAATGTATSTEKGRRIVGSERQTLAKDLVKRYTSGESIRALAASTGRSYGFVHRVLTESGVQLRQRGGARRRKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY46</accession>
    <name>U5VY46_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DeoR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_17680</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362101.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17680"/>
    <dbReference type="EnsemblBacteria" id="AGZ41814">
        <property type="protein sequence ID" value="AGZ41814"/>
        <property type="gene ID" value="AFR_17680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3584"/>
    <dbReference type="eggNOG" id="COG2378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041141_5_0_11"/>
    <dbReference type="OrthoDB" id="1654295at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028349">
        <property type="entry name" value="PafC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016838">
        <property type="entry name" value="PafC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="34095" checksum="42A9BE5B22DBF91B" modified="2014-01-22" version="1">MRADRLVATVLLMQARGRVTAAELAGELEISVATARRDLEALSAAGVPVYPQPGRGGGWSLVGGARTDLTGLTSSEARALFLLAGPAAAGAPAVRSALRKLVRALPDTFRADAEAATDAVIVDAARWGDRDKTRPAVVDLLQDAVVRRRKVTFGYASRGRPPAPRLADPLGLVDKDDLWYLIAGTDRGQRTFRVDRITDATVTDLVAERPAGFDLQREWDRVVGEVEQRRSLLSATVLITERLLPILRDHFGRHCETLGTLDDGRVQVRVAAPMAVSIAEQLAGWGASVEVIEPESVRAELARLGSELVARYGPAGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYI1</accession>
    <name>U5VYI1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SLATT_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360765.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12100"/>
    <dbReference type="EnsemblBacteria" id="AGZ40706">
        <property type="protein sequence ID" value="AGZ40706"/>
        <property type="gene ID" value="AFR_12100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2463"/>
    <dbReference type="eggNOG" id="ENOG5033401">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080426_0_0_11"/>
    <dbReference type="OrthoDB" id="1964908at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025325">
        <property type="entry name" value="DUF4231"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040884">
        <property type="entry name" value="SLATT_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14015">
        <property type="entry name" value="DUF4231"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18181">
        <property type="entry name" value="SLATT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="SLATT_1" evidence="2">
        <location>
            <begin position="149"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="30695" checksum="0DFA088B311C0444" modified="2014-01-22" version="1">MTSSLEAAWAQQSLWSQAATKLKRRLQTTRRAALGLAIGGAVLSATAATVGLESALGKVFAFAGAVLVGLSGLAGNAAGPTTMRDWTRVRSISEALKSAVYMSLAGFPPADLDAEIQDLEAGADDLRQYKVGIEPKQRPLPSIHDLESYLEGRVEAQIIGYYRKNARNLARTLERFRRLSIGLAITGLLLGAAAGTWEIDRLAGWVPVVTTIGAAVAAYVAAERYSYMLLEYSRTADELARIRHRRGRAASMTDEELVRHAENVISIQNEGWMAKLNEADEPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYR4</accession>
    <name>U5VYR4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360865.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12600"/>
    <dbReference type="EnsemblBacteria" id="AGZ40806">
        <property type="protein sequence ID" value="AGZ40806"/>
        <property type="gene ID" value="AFR_12600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2559"/>
    <dbReference type="eggNOG" id="ENOG5033HW5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136825_0_0_11"/>
    <dbReference type="OrthoDB" id="1719670at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="172" mass="19195" checksum="F4FCBAE9F4D1F9A5" modified="2014-01-22" version="1">MTIDLLIGAADNTVPITIQQRSSLAPADAFRLIMPIDLPTVFHRVAPFPGVRSVLNQTESWDHPGPTRNPQFDDGSQADEQLTECVEGVSFAYQLTGFTNVLSRLAAGVRGEWNFNPDGNGTLIRWTYEFKPLPGRRWIIAGPFAPLWRRYMVAALDRMVQWSEAAAQRPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ00</accession>
    <name>U5VZ00_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_19630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19630"/>
    <dbReference type="EnsemblBacteria" id="AGZ42198">
        <property type="protein sequence ID" value="AGZ42198"/>
        <property type="gene ID" value="AFR_19630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3984"/>
    <dbReference type="eggNOG" id="ENOG5030N35">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167347_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="89" mass="10355" checksum="306C169B098D8187" modified="2014-01-22" version="1">MNVTNWEYARLEYRAAGTFGADKYMDWTATFYHPGGTLRWGTDERFDDLRHLNRAGAAGWQAYDRAAIHVINEPHRLHGVTYSMRRPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ93</accession>
    <name>U5VZ93_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative metallopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_19610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19610"/>
    <dbReference type="EnsemblBacteria" id="AGZ42194">
        <property type="protein sequence ID" value="AGZ42194"/>
        <property type="gene ID" value="AFR_19610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3979"/>
    <dbReference type="eggNOG" id="COG3227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006520_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025711">
        <property type="entry name" value="PepSY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001842">
        <property type="entry name" value="Peptidase_M36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03413">
        <property type="entry name" value="PepSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02128">
        <property type="entry name" value="Peptidase_M36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666231" evidence="2">
        <location>
            <begin position="29"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="PepSY" evidence="3">
        <location>
            <begin position="186"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="957" mass="100051" checksum="A9E2EF523D050E27" modified="2014-01-22" version="1">MRRRRRSAVAGILAATTIFATAPIGAQAAPAGSEPAGATPLTGEHLDDEYAFVDNRGGTAAPSAGQRSLVRDDNLVTRWNKLGTPASVTPAKGRLAAGLKGDANSVARAYLKGSVGLFSLKTAAVDELETIAQTKVGKGTVVLMRQRYGGLPAAHDGLVAVAVKGSDVLQVTSTLSPSTAKPAAAKLSADDATAAALADAKIDRADLADSRVRLVAVPMPGADPRAAYEVVVMSKDAEHPLGYTTYIDARDGKVLLRENLVNFAEDDPHWKVFPATPPANGADTRQTWCATTATGCSRQVIDPATGKPWDIDPATGLPTFTTTGNSAETVSLWGNGSPATPATARPDREYVYPFTNQWNTSKCDPNSLTSPERADVDAATANLFAMHNRMHDWSYNLGFTEATWNLQKTNTVAYGKGADAERGNAQQGAATATTRNNANQATPPDGMQPTTNMYMWQPFAGSAYPPCVDGDYDMTVIGHEYTHAISNRMIAGPDSGISSSQGGAMGESWSDLLAMEYLFESGYTPRGNTPYVTGAYVTGDLGTGIRNYDMSNSPLNYSNVGYDRGAAVHSDGEIWSATNFDVRRALIAKYGSGTRALQESCAAGTTPATECPGNRRWAQLVFDSFLLAATGSISMLDMRDNMITADALRFDGANADLLWNAFATRGMGAGASSGPADADPVPSFASPYVENSEITFKALGEASGKPVRLYVGDYDFGAVPVADTDPATALPETVTLAPGTYKFLAIGTGYGHKRFTQTVYPGVTANVGVVMPANLASSASGATISGDGTNLGRLTDDAEGTAWTATGGAAGRQVTVDLPTDAAKLVSRVQLSAFPGAGAATSTMYSTVRQFEVLTCNAAAGADCATDAGYKVAYASPANALDTAQFRPKVPNLVLKSFPVRPTLATHVKLRVLTNQCTGNPLYAGEQENDPRSATDCTTASAAAGNVVAAEFEVFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZS1</accession>
    <name>U5VZS1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neutral ceramidase</fullName>
            <ecNumber evidence="3">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_19975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an N-acylsphing-4-enine + H2O = a fatty acid + sphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:20856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="EC" id="3.5.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the neutral ceramidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19975"/>
    <dbReference type="EnsemblBacteria" id="AGZ42267">
        <property type="protein sequence ID" value="AGZ42267"/>
        <property type="gene ID" value="AFR_19975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4053"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011300_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102121">
        <property type="term" value="F:ceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006823">
        <property type="entry name" value="Ceramidase_alk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038445">
        <property type="entry name" value="NCDase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031331">
        <property type="entry name" value="NEUT/ALK_ceramidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031329">
        <property type="entry name" value="NEUT/ALK_ceramidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670">
        <property type="entry name" value="PTHR12670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04734">
        <property type="entry name" value="Ceramidase_alk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17048">
        <property type="entry name" value="Ceramidse_alk_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0746">Sphingolipid metabolism</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665915" description="Neutral ceramidase" evidence="4">
        <location>
            <begin position="37"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Ceramidase_alk" evidence="5">
        <location>
            <begin position="42"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Ceramidse_alk_C" evidence="6">
        <location>
            <begin position="523"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="681" mass="71586" checksum="B401ABE23AF258F7" modified="2014-01-22" version="1">MIALGVCPEVPVKRLLPAFAALLLLVIPAPGRPAAAAAPGGYQVGVGIGDITGEAAEVGMLGYADPTQTTAGLASRLWARAFVVADGSGNHVAFVSTEIDFVTQAVQVEVLKRLRAKHGSTYTDQNVVLTATHTHSAPGGYSEYMLWNLTTLGFEAPVFEAIVAGIVRAVDSADADLAPGSVKIAEGNLAGANVNRSIEPFNANPAADKARFPDAVDTRMTVLRFEQGGRPVGLIDFFATHGTSMTPANHLISADNKGYAAHVIEDEVHGVDWAHRGDFVAAFAQTSAGDMSPNLRNGGAQGPTDDEFENTRIIGQLQAGKAQQLFDSATEELSGPIAARGRYVDFSGVQVGGAYTPDGQAHTTCPGALGQNFTAGAEDGPGPPIVDEGDLNTNPLLLVAGIVVNPTPAAVRACQAPKDVFLGTGSQNPPWTPQVMPLQVLRIGQLAITTAPGEFTIVAGQRVQDAVAAQLGGVTTHQILAGYANAYAGYVTTPEEYDLQHYEGAATHFGRYTQSAYAQELAKIAAALRDGQATPSAVQPPSLAQNRFSVRPGVVLDTPPLGKSFGSVVTQPAASYARGADVRADFVTGHPNNNLRDEGTFLEVQRQNGSIWQTVATDSEWQTLYRWKREYLGVSTAQITWTIPADAATGTYRIVHHGDAKGLTGTIRPFTGTSRTFTVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZW5</accession>
    <name>U5VZW5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362684.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20200"/>
    <dbReference type="EnsemblBacteria" id="AGZ42312">
        <property type="protein sequence ID" value="AGZ42312"/>
        <property type="gene ID" value="AFR_20200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4097"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_21_15_11"/>
    <dbReference type="OrthoDB" id="1115436at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="150"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="42370" checksum="A07EDA897FA37154" modified="2014-01-22" version="1">MSDALIDSLTAAVEARPDDLPLRLHLAELLVAAGRQGEAVGHAAQVLAREPANEVARGLMATALGSAPVPAPKAGVDWAALENEFEGVVPPRFTAAAPDEDGDNRAFDVERATITLADVGGMDEVKKRLELAFLGPLRNPELRTLFGKSLRGGLLLYGPPGCGKTFLARAVAGEMGAAFISLSIVDVLNMWVGSSERNLHDLFTAARSHAPCVLFLDEIDALGHKRSQLNSSAMRTLVNQLLTELDGVDGNNDGVFVLAATNAPWDIDAALRRPGRLDRTVLVLPPDAAARASILEYHLRDRPVAGVDLRALAAMTGHFSGADLAHVCETAAEFAMRDAITTGEVRMISQQDLAAAVREVRPSTDAWFATARNVALFGNESGEYDDLAAYLKKRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W044</accession>
    <name>U5W044_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361409.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41350">
        <property type="protein sequence ID" value="AGZ41350"/>
        <property type="gene ID" value="AFR_15340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3115"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_1_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="33"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="32362" checksum="EDE91F3C873BD012" modified="2014-01-22" version="1">MDEDPRRELADFLKARRTRIRPEDVGLEPGPRRRVQGLRREELALLAGVSPDYYQRMEQGRGVRPSEEVLDALARALSFTAEETRHLHKLAATARRPARRTRQRPPEVVPPTTLRLLSTMTGPAMVVGRHLDLLAWNPLAGALLGQFADLPRPARNMLWLLLHPDADLTCPDRAGTVGELTAMLRTAVAADPGHPRAVELVGELAVHSVEFRTLWGRHDIEETTRGRMRVVHPLVGELTLDWDAYRLPGAAPGPMIVAYTAPEGSADSERLQLLARLVDTPAPARIPVRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Q0</accession>
    <name>U5W0Q0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362934.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21460"/>
    <dbReference type="EnsemblBacteria" id="AGZ42562">
        <property type="protein sequence ID" value="AGZ42562"/>
        <property type="gene ID" value="AFR_21460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4353"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107158_0_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="202" mass="21734" checksum="4075AE87CAF93C4C" modified="2014-01-22" version="1">MPTGVALRDVRDQLFTAAERILLRDGPSALTSRAVTTEAGCAKGVLHRHFTDFDDFLAALVHDRIAKINHQADTLVRNAGTATVADNVTEALTTLFTSVALEILSLITFRDDLRARLRATQPAGFPLMADATRMIAAYLTAERDQGRLAGESDTDLLALMLIGTTHMLFAGRHGTPPDPHEVHRVVTATLGNTTPGMTPSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0T3</accession>
    <name>U5W0T3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361625.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16340"/>
    <dbReference type="EnsemblBacteria" id="AGZ41550">
        <property type="protein sequence ID" value="AGZ41550"/>
        <property type="gene ID" value="AFR_16340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3314"/>
    <dbReference type="eggNOG" id="ENOG5030UHS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1173466_0_0_11"/>
    <dbReference type="OrthoDB" id="2193263at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="26307" checksum="A3AD101520C01CEF" modified="2014-01-22" version="1">MTPALSADDQRAFESEVLAYFRSAFHEYCERAHRTPGLDLRGTVIEAQLVTASALQLVTVEIRRQADIFAGRLVRARAVDVRFPVQPRLGAPGYRLEVTPPIPGSEQTRPPQQRRPDSGRTLRLSYGSSLRWDFPLVSGPDWVPLGRSIPAQRGSGYLIPETVQAVPRGVLLEVRHQLGDTEVCRSEQRPEYIVEVDGLRLEPGYLVTAGETGSIVFSRADSAVSTRLTYRVDRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W149</accession>
    <name>U5W149_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_16690</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361706.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16690"/>
    <dbReference type="EnsemblBacteria" id="AGZ41616">
        <property type="protein sequence ID" value="AGZ41616"/>
        <property type="gene ID" value="AFR_16690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3384"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_3_0_11"/>
    <dbReference type="OrthoDB" id="944044at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="377" mass="39602" checksum="E402BD1F79EE9FF4" modified="2014-01-22" version="1">MSASLSSRKTQAALRTSARISAQSLLVLLMAWVALWILGRTWSVIWPLVIALLLTTLTWPVARFLRRHGWHPALAASTVTVGFLLVAAGIIVLIAVPVASQSGQLVTGVIDGIQQVREWASGPPLNIGDDQVTQAFDTGIQRVQDSVGSIITVTLTGVGTVVNGVVTTVLALFLMFFFLKDGPRFLPWLTRQLPGRLAVDIPIVTSRGWDTLGAFVRSQAFVGLLDAVFIGVGLWIVGVPLVLPLAVLTFVTAFIPIVGALFAGFVAVLIALVSNGPTDALIVLGIIVAVQQLEGNVFQPMIQSRGLGLHAAVVLLAVTLGGGLAGIVGSLLAVPVAALIAVVWNYIREQLSDPAPPEEVVPDTDPDPSDPASPQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1C0</accession>
    <name>U5W1C0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">8-amino-7-oxononanoate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17090"/>
    <dbReference type="EnsemblBacteria" id="AGZ41696">
        <property type="protein sequence ID" value="AGZ41696"/>
        <property type="gene ID" value="AFR_17090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3467"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_12_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="80"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="409" mass="44479" checksum="FDC75221DE60FAC4" modified="2014-01-22" version="1">MDHVLGNAKWADLERLRSGNPMYDATIEEIDGRRIRVGEHWLADFASCNYLGFDLEPEIMDSIGEQVRRWGTHPSWSRLLGNPALYPQIEEQLTELLDAPDTLVLPTITHIHMSVLPILAGQGLILLDSQAHKTIYDGAVYARGMGATLKRFRSGDAEQLAEMLRDAPAGMPKLVCMDGVNSMTGNAPDLATFARICREYDALLYVDDAHGFGVLGERAANEMSPYGIKGNAIVKYAGETYDNIVLVGGFSKSYSSLLAFLALPTWLKNHLKVSAPPYLYSGPAPTASLATVLAGMEVNAKRGDAIRADLYRKSMLVLDHVRALGVYTPNVGSTPVIELPLAEGEDIDVVGKLLWERGIYVTLAAYPLVPRDQVGFRAQVTAANTDAEIGELNAVITELAAAGRLRKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1E4</accession>
    <name>U5W1E4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17560"/>
    <dbReference type="EnsemblBacteria" id="AGZ41790">
        <property type="protein sequence ID" value="AGZ41790"/>
        <property type="gene ID" value="AFR_17560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3559"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085376_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="16"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="262" mass="28523" checksum="14CD33E43B703E98" modified="2014-01-22" version="1">MNDEFDAVLAAVGPRLRELRRRRGSTLTALAESTGIPISTLSRLESGQRRPALDLLLPLAKAFQVPLDDLVGAPASGDPRVFPRPVTRFGLTVIPLTRKPGGLQAFKMVLPAGPADREPDPKSHEGYHWLYVLNGRLRLVLGDQSLVLSSGEVAEFDTHLPHWFGTADTHPVELLSILGPQGERFHLRASSRRHGQEEEDKGRERRARADGVEAFPAGDQGAPVRRRGLRVPGEDGTVDRDGPAEGRHGPHQQGGESGVPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1H6</accession>
    <name>U5W1H6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Non-ribosomal peptide synthetase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_23680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363594.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23680"/>
    <dbReference type="EnsemblBacteria" id="AGZ43004">
        <property type="protein sequence ID" value="AGZ43004"/>
        <property type="gene ID" value="AFR_23680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4798"/>
    <dbReference type="eggNOG" id="COG1020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_2_2_11"/>
    <dbReference type="OrthoDB" id="572620at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensatn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010060">
        <property type="entry name" value="NRPS_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01720">
        <property type="entry name" value="NRPS-para261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="901"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1474" mass="157343" checksum="A9549322450FB10E" modified="2014-01-22" version="1">MQIIGGSIDGTVADRRWRSTGGPLTVAPATLPDVFATTARRDPAAVAVVFEDTEVSYAELDERANRLAHTLIARGVGPDRVVGLAVPRSIDMVVAELAVLKAGGAYLPLDPADPAERLAYMVGDARPACLVTTTEVAGRLPRFDDLPRLVLDDDNFAAELAAAPANDPARPGLRVESAAYVIYTSGSTGRPKGVLLTHAGVAKLLSTQFERFGLTPDVRVLHFASPSFDVAFWDLCLALLSGGRLVVVPADRRVAGPALTEYAQRHRANFMILPPALLAALPAGCELPPGILLAGTERVSPELVARWARGRRMFNAYGPTEATVNSTLGECDPEQVDGTSVPIGRPDPGTDAYVLDDRLQPVEPGVPGELYLAGPGLARCYVNRPDLTAERFLADPFGPPGGRMYRTGDLAAWRADGRLDFLGRVDDQVKVRGYRIELGEVESVLARHPGAGQVAVIAREDRPGDVRLAAYVVPAGDAAPDEQAQAQVAEWKELHELLYQAGRVERFDENFTGWNSSFDGSPIPLDQMREWRDGTVARIRALRPRRILEIGVGSGLLLSRLAPDCESYWGLDLSEEAIATLRGAVAGVPELADRVTLRAQPAHDLDGVPAGHFDAVVINSVAQYFPSADYLVGVLRAAAERLAPGGSVFVGDVRNLRLHRMLHTAIQAANGVSPAELDEAVDAAVRAERELLIDPGFFAGLPALVPAFRGAQVLVKRARFDNELSRYRYDVVLTTTPAVVPAEETLAWPGSLDAVRERLAERPARLRLTGVPNALLGGEPAPGVEDYHELAARHGCQAAVTWDGRSGRGDLDVVFERGEHEPAGAYRPADGDFPHANTPTAFTDPAALVKALRMHAESWLPAYMVPTGFVVLDRLPVLTSGKLDRAALPAPDYAALATGTAARTPREELLCELFADVLGVPGVGIDDDFFSLGGDSIVSIQFVLRARAAGLVCTSRQVFEHRTVAALAEVITVADTGTRDRPDDGDGDIPFTPILRWLDETGGDITAFSQSIVLTTPAGATEPGLTAVLNALTDRHDLLRARLVRATPDRPGLLRVASRGTGTPWLRRVVMPLGDPAELIAAEERAAADRLDPEQGVMAQAVFFDAGESTPGLLLLVLHHSIVDGVSWRILPADLATAWEQRAAGRRPALERTGTSFRRWATELKAAALRPDRAVELDLWRGILTGPDPALAGRALRPVDDLATVARHTVRLPAASTEPVVTTVPAAFHAGINDVLLTALALAVAGWRERTGRGAERSVLIALEGHGREEQVVADADLTPALGWFTSVFPVRLDPGDADLTDALAGGPAAGTALKRVKEQLRQIPDHGIGYGLLRYLNPDTEPELAGFAAPQISFNYLGRFSAGSKADFSPVAGHGILAGGFDLGMPVAPYTLEINAYVQDTAAGPELGVTWAYPRDLLDDAAVADLAAGWFTALEALAAHARGPAAGGLTPSDLTLTLSQDEIDEFEAEWETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1S2</accession>
    <name>U5W1S2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24510</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24510"/>
    <dbReference type="EnsemblBacteria" id="AGZ43168">
        <property type="protein sequence ID" value="AGZ43168"/>
        <property type="gene ID" value="AFR_24510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4964"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="322" mass="33849" checksum="ACE58032BD540FEF" modified="2014-01-22" version="1">MSIATVSRVLNGRGNVAPETRELVESTVARLAGPGPRPRGPARPIAGSVFVRCPYVLTDYFGVIVSSIAETLLLYGRPLVLDAGESAQRAPVLPRLPGRAGLAGAVLILPPEPAADIETLRSHRFPFVVVDPRLPPPRDVVAVSAAHAAGARSLTAHLTGLGHRRIGVVAGPREWLASDARLAGHNAALADAGVLSGPGLIRHVEPTVEWGRRAAGELLDRPDRPTALIGFNDKAAVGALQAAAERGLRVPHDVSIAGFDDIDLSRATTPMLTTVRQPLAELGRMAVTLLTRLLERHELEALHVELATELVVRDSTGAAPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W246</accession>
    <name>U5W246_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycogen operon protein GlgX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24665</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363789.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24665"/>
    <dbReference type="EnsemblBacteria" id="AGZ43199">
        <property type="protein sequence ID" value="AGZ43199"/>
        <property type="gene ID" value="AFR_24665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4997"/>
    <dbReference type="eggNOG" id="COG1523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011725_1_1_11"/>
    <dbReference type="OrthoDB" id="99080at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004133">
        <property type="term" value="F:glycogen debranching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02856">
        <property type="entry name" value="E_set_GDE_Isoamylase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044505">
        <property type="entry name" value="GlgX_Isoamylase_N_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011837">
        <property type="entry name" value="Glycogen_debranch_GlgX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02100">
        <property type="entry name" value="glgX_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="4">
        <location>
            <begin position="158"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="702" mass="78967" checksum="0EE00C0751301F58" modified="2014-01-22" version="1">MKIWPGNPYPLGATYDGGGTNFALFSEVADRVELCLFDDDGTETRIDLPEREALVWHGYLPRIVPGQRYGYRVHGPYDPAAGLRCNPAKLLLDPYAKAIEGHNEWNNALFSYHFGDPDSLNSADSAPYAMRSVVISPFFDWANDRPLRIPFHETVIYEAHVKGMTAQHPAIPEDVRGTYSGLAHPEMIKHFKDLGVTAVELMPVHQFVHDSGLIERGLSNYWGYNTIGFFAPHNDYSSFGGRGGQVQEFKSMVKALHAAGIEVILDVVYNHTAEGNHLGPTLSFRGIDNPAYYRLVDDNKQYYYDTTGTGNSLNVRHHESLRLIMDSLRYWVTEMHVDGFRFDLASALAREFHEVNRLAAFFDLVNQDPVVSQVKLIAEPWDVGDGGYQVGGFPPLWTEWNGKYRDSVRDFWRGEPASLGEFASRFTGSSDLYEIDGRRPIASINFVTAHDGFTLNDLVSYNEKHNDANGEDNRDGESHNRSWNGGIEGPTDDAEVVQLRERQKRNFLATLLLSQGVPMVAHGDELSRTQQGNNNVYAQDSELSWIDWEDARHHDVLTLFTARLTKLRADHPIFRRRRFFTGEPVPDTKVPDIAWLRRDGEPMTGEDWGAQSGMTMTVFLNGHGIPERGALGDPIVDDSFLVLFNPLADEVTFTLPPRSYGRGWEPVVHTGDPLLLARKRTVKAGGKLDVGGHTMVVLRCRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2D9</accession>
    <name>U5W2D9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Riboflavin biosynthesis protein RibD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25165"/>
    <dbReference type="EnsemblBacteria" id="AGZ43299">
        <property type="protein sequence ID" value="AGZ43299"/>
        <property type="gene ID" value="AFR_25165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5099"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="16"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="21068" checksum="EBFC26EE23412045" modified="2014-01-22" version="1">MSFRLHHVCLVMTVVEADISMSVDGFVTGPELEDFPGLGRGGEALHAWTGHPEGQALLDTIFGASGAVVTSRNVYDATDGWQAENGFFRMPVIVVTHREHEPVHKGETTFTFETGGIKAAIEAAKTAAGGKRVHIMGGASIVQQALRAGLVHRLRLHIAPLLLGSGTALFDGLPGPGLERLDTLDTPQATHVTYRVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W355</accession>
    <name>U5W355_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26885"/>
    <dbReference type="EnsemblBacteria" id="AGZ43638">
        <property type="protein sequence ID" value="AGZ43638"/>
        <property type="gene ID" value="AFR_26885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5448"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_8_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="98" mass="10938" checksum="A325C07A1C8AE078" modified="2014-01-22" version="1">MSLLRSAIGIVLRRLRLQQGRTLQDVAEAAGVSLAYLSEIERGRKEVSSEILAGICRALGLPLTDLLEQVRSELLRKGPQRPRVRMMICARSRLKVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W383</accession>
    <name>U5W383_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative pterin-4-alpha-carbinolamine dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_20620</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362768.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20620"/>
    <dbReference type="EnsemblBacteria" id="AGZ42396">
        <property type="protein sequence ID" value="AGZ42396"/>
        <property type="gene ID" value="AFR_20620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4181"/>
    <dbReference type="eggNOG" id="COG2154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109854_0_0_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="SSF55248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase_6" evidence="6">
        <location>
            <begin position="120"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24323" checksum="28901083E7522C70" modified="2014-01-22" version="1">MDMLRSAQIAEAGLTDWRKLAQGLHTRYLIAGFGTGARFVTAIAEAGDELGHHPRVSIGRGHIDLELVSDDAIYREDDGTEHIVEWVTQQDVDLARRITAIAAAHGLTPDPASVSELELGLDTAQSAVIAPVWAALLTGSADSKGRGTPGDEIRDATGRVPNLWFGDEAPGPRFHLEVYVAPETAAQRIADALAAGGTVVDDSNAPGQTVIADQDGNQATVCVDTAAVKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3D6</accession>
    <name>U5W3D6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_20870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20870"/>
    <dbReference type="EnsemblBacteria" id="AGZ42446">
        <property type="protein sequence ID" value="AGZ42446"/>
        <property type="gene ID" value="AFR_20870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4231"/>
    <dbReference type="eggNOG" id="ENOG5033THG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_570660_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665508" evidence="3">
        <location>
            <begin position="42"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="521" mass="53193" checksum="6451AD56EF571910" modified="2014-01-22" version="1">MRRPSYHRAAQRRGPDRRIIAAVAVLVAFGGVIGVTQISNADESKTVACQAPAADPAAPSGKKAASDPKVGTYTDKDGDVQHKGDGQLAKGEKAPEAQPVEECKTGNTTAKQVNGLTILANSCEDSDLQPHDGFQLGNRCVSTEFGEVGEAAKNPTLLITEFPETVKANTPFTLKVSTRNLVRDRFLAAGQGGYYVESSVLTAEGLVRGHFHTACRMLASTSEAVDPAPVPAFFVATEDKKGGADPDTVTIQVPGLPQEGIAQCASWAGDGSHRIPMMQRANQTPALDAVRVKVEGGQAEEPPAEEPENPPATTPPADNGGGDNNGGNNGGDNNGGGDNDNGNGNGGDNGQGDNGNNPNPGATATPKTDAGNTPPKTGTVTIPPKNDASAPKPAKTTKPAASTGDEATNDNNGDTGNGGDEATKDDEDAESPVQAVAGKTAKPTKKATKEPAAEVVTNTDDGAPDAQQAAAETTDVQAENKSKLALTGANSVTIILGGALLVLSGLVLVGATRRRRAAARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3J8</accession>
    <name>U5W3J8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase sigma-E factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_21220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362888.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21220"/>
    <dbReference type="EnsemblBacteria" id="AGZ42516">
        <property type="protein sequence ID" value="AGZ42516"/>
        <property type="gene ID" value="AFR_21220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4303"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="15"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="100"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="18899" checksum="52BD9CDA6A15FAD9" modified="2014-01-22" version="1">MRQDEEFAEFARAAVERLRTTAFMMCRDWHLAQDLTQTALTRLYLGWKQAREADNLTAYAQKVLLRAYLDHRRRRSTSETVTGVIHDTGYRQSPDLRLTMLDALAQLPARDRAIVILRYFEDYRVEQVADVLDLPVSVVKSQTRRSLAKLRELLAADQLALFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4H7</accession>
    <name>U5W4H7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Malonic semialdehyde reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_23125</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363406.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23125"/>
    <dbReference type="EnsemblBacteria" id="AGZ42895">
        <property type="protein sequence ID" value="AGZ42895"/>
        <property type="gene ID" value="AFR_23125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4686"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084441_0_0_11"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02148">
        <property type="entry name" value="RutE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023936">
        <property type="entry name" value="RutE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="22296" checksum="0CC70658C10DD037" modified="2014-01-22" version="1">MDTRSEPTLDRLDAKAQHLLFTGARTANTFADTPVTDAELSDIWELAKWAPTSANSQPLRVLFVRPGPGRDRLVKHMNEGNRAKTASAPAVAVLAQDTEFHTHIPTVLPFRPELKDVFAGNDTMRTSTATFNATLQAGYFILAVRAHGLAAGPMSGFDAAGVDAEFFPDGRHRSILVVNIGHPGKDPWLGRLPRLPHDDVVQWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4L5</accession>
    <name>U5W4L5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363501.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23350"/>
    <dbReference type="EnsemblBacteria" id="AGZ42940">
        <property type="protein sequence ID" value="AGZ42940"/>
        <property type="gene ID" value="AFR_23350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4732"/>
    <dbReference type="HOGENOM" id="CLU_2930640_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="60" mass="6160" checksum="1256E0628C7316C7" modified="2014-01-22" version="1">MIALPILLLAAVIGVFLAAADGPYEHDKVRLERVSVVLIAAAAVIAAAGVVVVVRDWVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4L8</accession>
    <name>U5W4L8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chloroperoxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28990"/>
    <dbReference type="EnsemblBacteria" id="AGZ44059">
        <property type="protein sequence ID" value="AGZ44059"/>
        <property type="gene ID" value="AFR_28990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5874"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="77"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="322" mass="34162" checksum="F4591106B9BA1F3A" modified="2014-01-22" version="1">MNEQNENHPAGRRGASRRTVLGAGTGAALTAAAVVGTGSSPANAAPHAGGGRSGTRIPVAPGVEVNVSDLDGGRRGTVVFVPGWPLASTTFEYNYLFLADHGYRAIGLDLRGFGLSDAPYGPYTYDVWARDILKVLEALSLRDVTLVGHSMGGAIALRHAARFGSRVGKLVLAEASAPRYVYGPQSADLAAGINGLISGYATDRAATIRGLTANFFSTHTDITTDPFLQFFERQCLDQASLQASRAGLIALRDDDLTADMARVKVPTRVFHAINDKIVPLDHGQALAAGIRDARLITFATAGHAVYVDERDKFNAELLTFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W507</accession>
    <name>U5W507_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_29790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560556.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29790"/>
    <dbReference type="EnsemblBacteria" id="AGZ44219">
        <property type="protein sequence ID" value="AGZ44219"/>
        <property type="gene ID" value="AFR_29790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6036"/>
    <dbReference type="eggNOG" id="COG0669">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100149_1_1_11"/>
    <dbReference type="OrthoDB" id="1846503at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001980">
        <property type="entry name" value="PPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF1">
        <property type="entry name" value="PTHR21342:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01020">
        <property type="entry name" value="LPSBIOSNTHSS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01510">
        <property type="entry name" value="coaD_prev_kdtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="8">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="133" mass="14768" checksum="B93B9A57F5852AD7" modified="2014-01-22" version="1">MHAVYPGTFDPFTAGHADVVDRVRRLFDRVTVLVAVNGEKQLAATLQQRSEALRRLLPEDWNNVTVTAWAGLTAAFCHEHQAGVIVRGIRNHDDLRHESELAAMNEHLGIPTLLVPARPELATVSSTADRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>U5W543</accession>
    <name>U5W543_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Adenylate kinase</fullName>
            <shortName evidence="6">AK</shortName>
            <ecNumber evidence="6 8">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">adk</name>
        <name evidence="10" type="ORF">AFR_30020</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="6 8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560601.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30020"/>
    <dbReference type="EnsemblBacteria" id="AGZ44264">
        <property type="protein sequence ID" value="AGZ44264"/>
        <property type="gene ID" value="AFR_30020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6080"/>
    <dbReference type="eggNOG" id="COG0563">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_1_2_11"/>
    <dbReference type="OrthoDB" id="1491686at2"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043231">
        <property type="term" value="C:intracellular membrane-bounded organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036193">
        <property type="entry name" value="ADK_active_lid_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="PTHR23359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57774">
        <property type="entry name" value="SSF57774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ADK_lid" evidence="9">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="6">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="6">
        <location>
            <begin position="122"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ44264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="24585" checksum="42633A6E4BA86BA5" modified="2014-01-22" version="1">MRKYVIMGVQGSGKGTQSELLCDDLDLVHISVGDLFRWHVQNHTKLGAQVRRTMKSGELVSDDLVESVVRDRLDSHDWNFGFVVDGFPRNGRQAEFFMESYDIDGVIHLELSDDEVRRRVLSRRLCPGCGMDYNLIADRPKVEGRCDICGTTLITREDDTPEALAARLRDYYEKTKPVLELFRRKEVVHDVDAAPAADEVQASIRSTLGLPAYEPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W555</accession>
    <name>U5W555_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor IF-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">infC</name>
        <name evidence="8" type="ORF">AFR_30465</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the IF-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560690.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30465"/>
    <dbReference type="EnsemblBacteria" id="AGZ44353">
        <property type="protein sequence ID" value="AGZ44353"/>
        <property type="gene ID" value="AFR_30465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6168"/>
    <dbReference type="eggNOG" id="COG0290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054919_3_2_11"/>
    <dbReference type="OrthoDB" id="1760144at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00080">
        <property type="entry name" value="IF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036787">
        <property type="entry name" value="T_IF-3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001288">
        <property type="entry name" value="Translation_initiation_fac_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019815">
        <property type="entry name" value="Translation_initiation_fac_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019814">
        <property type="entry name" value="Translation_initiation_fac_3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938">
        <property type="entry name" value="PTHR10938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00707">
        <property type="entry name" value="IF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05198">
        <property type="entry name" value="IF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54364">
        <property type="entry name" value="SSF54364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55200">
        <property type="entry name" value="SSF55200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00168">
        <property type="entry name" value="infC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF3_N" evidence="7">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="IF3_C" evidence="6">
        <location>
            <begin position="78"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="22356" checksum="FFD70ED861CFC2F9" modified="2014-01-22" version="1">MNEQIRAREVRLVGPEGEQVGIVPLERALQLAADVDLDLVEVAPMARPPVCKLMDFGKFKYESALKAREARRNQQQTVIKEMKLRPKIDPHDYETKKGHVVRFLKAGDKVKVTIMFRGREQSRPELGFRLLRRLSEEISELGFVEASPKQDGRNMIMVLAPHRATKAAAAATIAANARPPREPREGDGAEAPPAAADTTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5L6</accession>
    <name>U5W5L6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative metallo-beta-lactamase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363870.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25070"/>
    <dbReference type="EnsemblBacteria" id="AGZ43280">
        <property type="protein sequence ID" value="AGZ43280"/>
        <property type="gene ID" value="AFR_25070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5080"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_4_11"/>
    <dbReference type="OrthoDB" id="733552at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="15"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="233" mass="24848" checksum="854F60DBCB482447" modified="2014-01-22" version="1">MREVVDGVFELGIGYVNMHLVVTDDGVVLVDTGLPGKSAKIDQALHDLSRKIGDVRTVLLTHHHPDHTGNVADLRRRSGARVIAHQADVPYVTGDKRAVIKHPVLKVVSLLMGKPELSPVDDVITTDGSSPLPGFTALHTPGHTAGHVSYLLDRDGGVLFTGDAAAGKRGGQVGSTPKMVSDDLAQQARSVARLADLDFEHAVFGHGNAVSGRAVDRFREYVVQRTHRGLTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5M1</accession>
    <name>U5W5M1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dyp-type peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_25095</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DyP-type peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363875.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25095"/>
    <dbReference type="EnsemblBacteria" id="AGZ43285">
        <property type="protein sequence ID" value="AGZ43285"/>
        <property type="gene ID" value="AFR_25095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5085"/>
    <dbReference type="eggNOG" id="COG2837">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_453189_0_0_11"/>
    <dbReference type="OrthoDB" id="837319at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006314">
        <property type="entry name" value="Dyp_peroxidase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51404">
        <property type="entry name" value="DYP_PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="602" mass="65065" checksum="CF99377C0C0572D8" modified="2014-01-22" version="1">MSVGTRSVTVFARIADGRAEAVRERLRAYEKDSPFARVAGTHFARLVVMEHAVPQERPTVWRRPARLLDLLAHFGRGPRPDDLPHPYLMMTATVDGEPADFFRRLRHLGPDADTIWGDCTDYPRHYEEASFVAFFTERSVRANYTFAGAPDGSVTEIRDALLLRKQLAAFAATASDVSDDALLRRFRTATEPVVSPVAAPGPEIEAKDVQGLVLRGFGHHGVSAHLFLRVTSGEDARRWLSTVVPEVTTAAEIPDRPDRALHLGLSHSGLARLGVADSSLNAFPEEFREGMYAREDVLSPGRGTGPWQAPFDGPGAVDVVLLLSATDSTTLDPWLDRLRQDLDGLVPAGEQRGARLPDFVEHFGFADGLSQPRVAGYGKDGHGEVLPAGEFVLGLPDVDGDTAGRGLPSGLTRNGSFLVYRKLEQDVPAFRQLTEAAAGPFADGAEDVAAGLVGRRRDGAMLSPCPAGAHVRRANPRDSLPGGTKLTRRHLMLRRGIPYGPYLPPGADDDGADRGLLFLALVGDLGRQFEFVQTEWLADGNVFGRGAEEDIFTTAGGPRARILLEGSPPAYVSVPRPLVTCRGGEYFLLPGLDALRSLATTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5P8</accession>
    <name>U5W5P8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24925"/>
    <dbReference type="EnsemblBacteria" id="AGZ43251">
        <property type="protein sequence ID" value="AGZ43251"/>
        <property type="gene ID" value="AFR_24925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5049"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_283685_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="918" mass="98668" checksum="F7E494A6B3BFC607" modified="2014-01-22" version="1">MTAVLPPRHVHANVPIVSAADLQLVESPLVGDACEPPFGSLPDVVRGREAQIGELRVLAAAGGVAVLSGEIGCGKTTIVLAAARALAGDHTVFWVPAHQRASFVDAMAAVASRLRVSGAEITRAREEGSADLAWRLLTAPPDAATGSARNILVIDNADDPGLAADVIRRARGLDPERWLTIVTTRIVTGLAGSGPATTLTIDRLSDEDCAHLLLDRIRELDDHRRFRALPAALEAVGLLGRVPLAVHLAGSAQGSSVARHTLSGYVGELSRAPVGRPGLRADEPAYHLLRALRPALDAFGAEEKDTALRLLAVLAGSAPGRPFPLGALVVATGPAETPLAYRSVRLLAQSGIVTIGTDDNLHVATIHPLVARAAAHLDGGPATAAQSVRGAAAGALDAMTRPGGSTRPGDDEWQLWRLLIPHVRHVLTSPGSDGDVVVLRVAHRAVRQLTSHGMHRAAAQLAEDALGRTGSLPAGQEGAHRTALLDRGLTLQACGDDLKGAFRHLSDVALRTRRDCPPDDPEAMHAGHCLAAVLHELGDLTEAEQLFRPVLDARRDVLGAGHPDTLTTMQCLAAVLQAAGRAGEAEEMLQQVHSGRVRQLGPDHPDTLTTRHSIAYARQAAGGPGAERDFAQVLADRRRVLGETHPNTLITRHNLAWIEQAGGNYAAAEDQFRAVLQLQIPRLGRAHPHTVATAANLAWDLLQQRQFTTARLLFTQVLKIRTGRLGSLHPDTQTTRGNLGWLTYEEGDFVRAERRFRKLLGDRTRLLGDHHPRTLTTRHNLALSLRSQRRLPEARDEFISVLGDQERVIGGSHDSTLATRYNLAVTLRMLNDDMWLTEAMSLLDSVLATLRGRPNPLLRQTKRELVTLLAIRSGRADVHELLDDATFEAPPTTTADDPLVRRFVDDDIDDFVDPDLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W673</accession>
    <name>U5W673_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tetratricopeptide domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_25935</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25935"/>
    <dbReference type="EnsemblBacteria" id="AGZ43451">
        <property type="protein sequence ID" value="AGZ43451"/>
        <property type="gene ID" value="AFR_25935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5258"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_15_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="155"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ43451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="29708" checksum="19F3A8F1EC28790C" modified="2014-01-22" version="1">MVLPHALAAIEQWEKQAWTPDRVTAEHCAYLMDRAGAYLEVQGQLTEARDLMERGLILTERTVDPGHPAIPARLHNLSRSVNLTGFPEKAQPLAERALALAEEAHGCNSSEVAFNLINLANILGEQGNFDEARRLLERALAIGESELGLSHPSVAILLNNLGLILEATGQFDEAQEHLERAVALSRENLGEDNPGLAVSMINLGNLLRVTGRLEEAHDLSRRATDVLERAYGPDHPELANSLVSLAEILEDLGRADDAAPLRERAARIRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6E1</accession>
    <name>U5W6E1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative HxlR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560885.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31460"/>
    <dbReference type="EnsemblBacteria" id="AGZ44552">
        <property type="protein sequence ID" value="AGZ44552"/>
        <property type="gene ID" value="AFR_31460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6367"/>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_2_2_11"/>
    <dbReference type="OrthoDB" id="2057904at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="14"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="13112" checksum="A15F1D0B860B96C4" modified="2014-01-22" version="1">MRAPLDPDLFDAVCPSDLTPIRFGDKWAGMVIRCLEGGPRRFSELRVPLRGITAKTLTHALRALERDGLVQRSDERLYALTPLGRSLLGPMDQACAWAREHWEELLDAREAGTAAVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6E4</accession>
    <name>U5W6E4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-L-isoaspartate O-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-beta-aspartate methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_31485</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560890.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31485"/>
    <dbReference type="EnsemblBacteria" id="AGZ44557">
        <property type="protein sequence ID" value="AGZ44557"/>
        <property type="gene ID" value="AFR_31485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6373"/>
    <dbReference type="eggNOG" id="COG2518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037629_0_1_11"/>
    <dbReference type="OrthoDB" id="1948290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PTHR11579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="37854" checksum="487C9F82A56BDF63" modified="2014-01-22" version="1">MTDLRRRYVDQIRRNWASLPPELAAAFAGVPREAFVTGGFQRRDGSWARPGSPGFLDAVYTDDVLITKVDGRVPISSSSQPSLMAIMLAALEVRSGDRVLEIGAGTGYNAALLAALGATVTSVDVQADVADKARAALTTAGIDGVRVVAGDGYAGMPGERFDRAIVTVGVAGISPQWLVQTGPGPVVAPVEHAGTHPVLAVRGPAEGPVTATAICASGFMSASGPLTATHARSHPHPAPSGAVGAVTEVAPRRWAEPLDSLVYRDLWYAAGVWSDRATHAALPGRDQSALMLLDEAGTGGAAILPDGCVLAGGVHASAYAEQAVEILDRWEAADRPPMQAWRIGLQLTGDPDAPIWAPATWTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6J0</accession>
    <name>U5W6J0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32555"/>
    <dbReference type="EnsemblBacteria" id="AGZ44769">
        <property type="protein sequence ID" value="AGZ44769"/>
        <property type="gene ID" value="AFR_32555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6588"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054927_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF58" evidence="1">
        <location>
            <begin position="25"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="32031" checksum="D627DA43350D147A" modified="2014-01-22" version="1">MEWRLGRRLDGRLQGAYRTVWHGTGIDFTDLRAYTPEDDVRHIDWNVTARLDEPFVRQYAEDRELTAWLIIDRSASMRFGRAVDGRQGKESVATELAVSLGRLFAQGGNRVGAILYDNGSNKIIPPRSGRDQILRIARELMRAPAATGAGATTDLAAMLRLAASTTSRRRSLVFVMSDFIGDPGWDRPLAMLTHRHEVVVVRVVDPAELELPDLGVILVEDAETGEQLLVDTSDPLLRGRLAAQVGAREEELAEGMRRAGVDAHRMTTDQDLLATLVDMVRHSGRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6N4</accession>
    <name>U5W6N4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364411.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26570"/>
    <dbReference type="EnsemblBacteria" id="AGZ43576">
        <property type="protein sequence ID" value="AGZ43576"/>
        <property type="gene ID" value="AFR_26570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5386"/>
    <dbReference type="eggNOG" id="COG2047">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055821_1_0_11"/>
    <dbReference type="OrthoDB" id="834483at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008492">
        <property type="entry name" value="DUF774"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028754">
        <property type="entry name" value="UCP028754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="31959" checksum="CF004014C121E5C8" modified="2014-01-22" version="1">MTEFDGLPLLRSPVAIAAFEGWNDAADASTAAVEHLEQVWQAREITTIDPEEFYDFQVSRPTITMAEGETRKIEWPTTRFSVASPPGTDRDVVLIRGIEPSMRWRTFCEQILEVCHSLEVNRIVLLGALLADVPYTRPLPISGTASEKEVAEKYKVVPTRYDGPTGIVGVLQEAAGRAAVDALSFWVHVPHYANNPPCPKATLALLSRLEDVLDLPVPLADLAEESDEWEKRVRAAAEQDSELGDYVRELEERVGDEGIKPLTGDEIASEFEKYLRRRGGSAGPTAGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Q3</accession>
    <name>U5W6Q3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">APHP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33150</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33150"/>
    <dbReference type="EnsemblBacteria" id="AGZ44888">
        <property type="protein sequence ID" value="AGZ44888"/>
        <property type="gene ID" value="AFR_33150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6708"/>
    <dbReference type="eggNOG" id="COG1572">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008465_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd14490">
        <property type="entry name" value="CBM6-CBM35-CBM36_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033801">
        <property type="entry name" value="CBM6-CBM35-CBM36-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666139" evidence="3">
        <location>
            <begin position="32"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="4">
        <location>
            <begin position="25"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="4">
        <location>
            <begin position="351"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="253"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1279" mass="132609" checksum="DB259AEE5935DCA7" modified="2014-01-22" version="1">MSRFRQKILATVVATGLVTALIQVTAPISPAAAAGPNLAAGKTFSASSFSDVYGAGNAGDGNPSSYWESQNNAFPQWLQVDLGTAVSVNQAILKLPPATAWQTRTETLAVQGSTTGSNFTDLKASAGYTFNPASGNTVTIDFGATTTRYVRLNITGNSGWPAGQISELEVYGPATGDTQAPSAPGGLAYSQPASGQIRLNWNASSDNVGVTGYEVYANGTLRTTVAGNVLTYLDNQPDSATVAYYVRAKDAAGNTSGNSNTVTRTGQSGDNSAPTTPGTLSYTQPATGQIRLAWGASTDNVGVTGYNVYANGTLKTTVTALTYTDSQPASATVAYYVKARDAAGNESAASNTVTRTGTVTGGSNLAVGKPITASSSIFTFVAANANDNDTATYWEGSAYPAALTVSLGANATTSNVVVKLNPASDWGTRTQTFSVLGREQSASGFTTLVPSATYTFNPANGNAVTIPVSATAADVRLNFTANTGAPSGQVAEFQVIGVAAPNPDLTITGLSFSPASPVETDTVTLSATVRNAGTAASPASNVNFYLGTAKVGTANVGALAAGASATVTASIGARDAGTYVLSAKVDEANTVVEQNDANNSYTNPTNLVVSPVSSSDLVASVVSWSPGNPSAGQTVTFSVTIKNQGTAASAGGAHGVTVTVLSDSGATIKTLTGSYSGTLAAGASSPSINLGTWVAANGKYTVRTVLADDANELPVKRTNNTSEKPFFVGRGANMPYDMYEAEDGQTGGGASVVGPNRTVGDLAGEASGRKAVTLNSTGSYVQWTTRASTNTVVARFSIPDNTTSSINVYVNGTLNKTLPLTSKFAWLYGNETAPQNSGSDPRHIYDEANILLNGTVAAGSVIKLQKDSGNGGNIAIDFINTEQVAPVANPNPATYVVPTGFDQQSVQAAFDAARQDSSKVGVYLPAGDYSTAQKFQVYGKALQVVGAGPWYTRFYTPQNQSETDAGFRADATANGTTFKNFAFFGNYTNRIDGPGKVFDLANVSNMTIDNIWAEHTVCLYWGANTDRMTIKNSRIRNLFADGVNMTNGSTDNLVDNNDARATGDDSFALFSAIDAGGSDEINNVYSNLSTTLTWRAAGIAVYGGYANTFKNIYIADTLVYSGITISSLDFGYPMNGFGANPPTVFDNISIVRAGGHFWGSQVFPAIWVFSASKVFQGIRVSNVDIVDPTYSGIMFQTNYVGGQPQFPVKDTVFTNVSITGARKSGDAFDAKSGYAIWANPMPEAGQGPAVGSATFTNLTLSNNFKDIENPTSTFTITRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6U1</accession>
    <name>U5W6U1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase GroES domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561260.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33325"/>
    <dbReference type="EnsemblBacteria" id="AGZ44923">
        <property type="protein sequence ID" value="AGZ44923"/>
        <property type="gene ID" value="AFR_33325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6744"/>
    <dbReference type="eggNOG" id="COG1064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_11"/>
    <dbReference type="OrthoDB" id="1859760at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="10"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="346" mass="35764" checksum="172C37ED13028FE6" modified="2014-01-22" version="1">MRAVIYDRFGDLPEVRTVPDPEPPPGGVVVAVHATGLCRSDWHGWRGHDPDIQLPHVPGHEFAGVIAGVGAGVSDWQVGDRVTAPFILACGTCPACQAGDQQVCHRQLQPGFTHWGSFAEYVTVPHAMTNLVRVPDDLDFATAAILGCRFATSFRAVVAQGRVRAGEWVAVHGCGGVGLSAVMIAAAAGAQVVAIDVSDSALALAARSGATTTLLAPSVAAIRTATSGGPHLSIDALGSQSTLTASVESLRRRGRHVQVGLLPAVSGRPTVPMELVVAHELELLGSHGMQAHAYADLLPLIAAGKLRPSDLITGEITLEDAPAAMAAMDGPVPPGVTIIRPPTPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W718</accession>
    <name>U5W718_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27595"/>
    <dbReference type="EnsemblBacteria" id="AGZ43780">
        <property type="protein sequence ID" value="AGZ43780"/>
        <property type="gene ID" value="AFR_27595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5593"/>
    <dbReference type="eggNOG" id="ENOG5031WGF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1187897_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="255" mass="27899" checksum="7CB291452DBF9458" modified="2014-01-22" version="1">MGTHLEQWWAQFLEASDRFDAAYVVEGISDLVGPYIKAPLLRREVKIAADNVVRYLERPSSKERAEQAREASDRLVRTLDRIEDRSTGSAISTEEAGIIGLALRGEYAAAAAAAEPLVGTSKLQRLFVTALRLERFDIPMALRLLDGGQTPREAVRSGHLLGKYSWWPSWLLRIVTERALEGTLDEETIVALDTCAYAELTPIQANLARKLLSGNESLIDTAAHRLAGLGETEAAAALRQGDINAVALAARLIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7A4</accession>
    <name>U5W7A4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lipase 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560212.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28070"/>
    <dbReference type="EnsemblBacteria" id="AGZ43875">
        <property type="protein sequence ID" value="AGZ43875"/>
        <property type="gene ID" value="AFR_28070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5690"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029538_3_0_11"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triglyceride lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005152">
        <property type="entry name" value="Lipase_secreted"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34853">
        <property type="entry name" value="PTHR34853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03583">
        <property type="entry name" value="LIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029171">
        <property type="entry name" value="Esterase_LipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665774" evidence="2">
        <location>
            <begin position="31"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="37873" checksum="6A9FBC79FEC85DE7" modified="2014-01-22" version="1">MNSALLKWVAAVNVALVCAAILLFAGGPAAATVAPGTVLVTTTATLPPELAPLATGKRIAYVTTAPNGAILTATGLVLTPKTNKKNKTVVWGHGTTGLADKCAPSASQNVFWPEARQAVAALLNKGWTVTAPDYPGLGVPGNHPYLIGLSEARSMIDSVKAARSLDSKLTTQYALDGHSQGGQGALFAGQIAPSYDGSLVLKGVASIAPVSNAQIFAPLVPGTPGQGYLVMALYGLNAADPTFDPNSVLAAPAKQKAPVLQNGCLYEILDAYAPLTPEQLLVGGALPESVVTKLANYVDPARSAPTAPVLLIQGTDDEAVPYGLTAGPLLDELTAYGTQPVDFVSVDGANHDGAVFATTGQVATWIAARFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7H2</accession>
    <name>U5W7H2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560238.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28200"/>
    <dbReference type="EnsemblBacteria" id="AGZ43901">
        <property type="protein sequence ID" value="AGZ43901"/>
        <property type="gene ID" value="AFR_28200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5716"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026152_4_2_11"/>
    <dbReference type="OrthoDB" id="848990at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF58" evidence="1">
        <location>
            <begin position="189"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="39667" checksum="BB6A78C210B772E7" modified="2014-01-22" version="1">MRVTPRGYALLVVAVLLLVAGFRGRYPLAGVLGATLLGAVLSAVVTTRGSLRITVRRTVYPSRVTRDRPALARLEVTNPGTRRQAPLTATDQAGPVTRTVQVRALLPGAGAVLHYELPTGVRGKWPVGPLTLERRDAFGLARNTLTAAGETTTLWVHPRRVPARVLFGGQRRHHHEGAVTDSALRGSVELTDVREYVPGDEVRLLHWKATARTGRLMVRDLADPQRSRFTVLLDTRSPASGADRFEELVETAASLLGASVGAGQHTSLLTSSGLRLTVEGTAEGARRLLDALSEVARDDRPVDLPEWRGGTLVVVCGAGPGLVPAGWLRHRFTQVHVVAMNGPLDVPGARMLRAETAGDAVRRWNEATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7M5</accession>
    <name>U5W7M5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561530.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34685"/>
    <dbReference type="EnsemblBacteria" id="AGZ45193">
        <property type="protein sequence ID" value="AGZ45193"/>
        <property type="gene ID" value="AFR_34685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7018"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045252_0_0_11"/>
    <dbReference type="OrthoDB" id="1232102at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="294" mass="32292" checksum="E569397CE196C82C" modified="2014-01-22" version="1">MVRRSSLQEIREQTYKVRDAWWTVLLVDPLAARLVRLVAPYRWITPNLLTGLATLLGVAAAACFVAPGLTDQNRWWLAAGAALFHLSFVVDCMDGKVARLNGTGSLFGAWFDFMFDRLRVFMCAVALFGGQFLRTDEVGYLWTLIIVTFLDLFRYVNSQQMTKTRETMRASLEEVRGPLPPAESEAAVNLPPQGPRARLRAALLRHRIRTHLVSGIEFEMAVFIIGPLTGWIIGTSLVAGALMVAFELLLIVKLWRATRGFPVALAKAQAAAALLKPGAPAQASAPGADRVSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7M9</accession>
    <name>U5W7M9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560337.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28695"/>
    <dbReference type="EnsemblBacteria" id="AGZ44000">
        <property type="protein sequence ID" value="AGZ44000"/>
        <property type="gene ID" value="AFR_28695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5817"/>
    <dbReference type="eggNOG" id="COG1408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2566146_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="81" mass="8588" checksum="7DCC5A015BEB9E53" modified="2014-01-22" version="1">MAPLAQLRARHGSFFVTGHTHGGQLWPGPYVAELVMPTVAGLHRYGDTQLYVPRGAGAWGPPVRVGAPSDITAIELASRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Q5</accession>
    <name>U5W7Q5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28600"/>
    <dbReference type="EnsemblBacteria" id="AGZ43981">
        <property type="protein sequence ID" value="AGZ43981"/>
        <property type="gene ID" value="AFR_28600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5799"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="24131" checksum="B1B3D77CF7F99119" modified="2014-01-22" version="1">MTTDVLAVDDVSMVYRGGVTALDRVSLTVAAGDLLAIVGASGSGKSTLLNVMGTLDRPTHGTVTVQGYDVSTLSDRRLSALRGRSIGFVFQQFHLTDGFTATENVATGLLYAGVPRRKRRAVAQEALDRVGLGHRLEHQPQQLSGGEKQRVAIARALATRPALVLADEPTGALDTATGHAVLDLLVRLHTEGTTIAVITHDRDIAAALPRRVELRDGRIIADTRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Y1</accession>
    <name>U5W7Y1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561449.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45112">
        <property type="protein sequence ID" value="AGZ45112"/>
        <property type="gene ID" value="AFR_34270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6934"/>
    <dbReference type="eggNOG" id="COG5330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023855_0_0_11"/>
    <dbReference type="OrthoDB" id="516582at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="496" mass="53636" checksum="B591E248133A6BAB" modified="2014-01-22" version="1">MTAVDPVLEGLAVNPALPEELLLRLVADGRAADGLAARASLPAAVVAAMLDHPAPRMRAAVGSNTSVDPQTRLSLLNDPDPWVRSRLEKQRELPLPEWALSRIFARLARSVDRGLLSRQEMLGEVFDEAGREGRLRPLLVRHEDEQFRLIACHFTPYLDEPDQQALLHDESPTVRAAAAAKLYPPVVADLNGAGGYERVALLGRPLTRELIEEVLAAGDEEDLLALARNTTLPADVVAALVTHDLHRVRRETARRDDLTSTQAVRLGADPDPSVRAEVSVHPVLTEAQRAVIAVPPETTFCGCESVPVVPGQSAVWARSVNPLLRRRAAADPLLPAEIAAVLADDPDAVVRGLLAVHHPAASPALKLRTFLEHDGCARRRLRFPVTDSARFATSPDAAVRQLVARDPDADPALVEELLTDPDESVRRVMAGCARLPVHRIVALLDDPELSTAAAANPSLPRAEMERLIRRAEEVRQPPVDARRAGPTAPGAAPRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Z7</accession>
    <name>U5W7Z7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron-dependent repressor ideR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_35435</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35435"/>
    <dbReference type="EnsemblBacteria" id="AGZ45343">
        <property type="protein sequence ID" value="AGZ45343"/>
        <property type="gene ID" value="AFR_35435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7171"/>
    <dbReference type="eggNOG" id="COG1321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="SSF47979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="8">
        <location>
            <begin position="4"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="232" mass="25588" checksum="8813C03B8C49A571" modified="2014-01-22" version="1">MNDLVDTTEMYLRTILELEEEGVPPLRARIAERLHQSGPTVSQTVARMERDGLLTVENDRHLLLTEEGRVAAVAVMRKHRLAELLLVNVIGMPYEEAHEEACRWEHVMSDSVERKVYELLNKPTRSPYGNPIPGLEVLGLPAEEPLGSSSTVINSGERNLAFPGLSGSVVVRRICESVQTNAGVLRQLHAAGIDPGATVTVAQERDGVTIDRSGDKIRLPREVASRVFVDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W800</accession>
    <name>U5W800_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative GDSL-like lipase/acylhydrolase with Ricin-type beta-trefoil lectin domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560432.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29170"/>
    <dbReference type="EnsemblBacteria" id="AGZ44095">
        <property type="protein sequence ID" value="AGZ44095"/>
        <property type="gene ID" value="AFR_29170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5909"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044083_0_0_11"/>
    <dbReference type="OrthoDB" id="1622507at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666419" evidence="1">
        <location>
            <begin position="32"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="252"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="367" mass="37756" checksum="A422900231F4F0FC" modified="2014-01-22" version="1">MHGRRRWILGAALAAILTLAGTFTQTAAAQAESNGGVRVMPLGDSITEGTQVPGGYRIGLWQRLSGAGYRVDFVGTQFNGPAALGDHDHQGLPGWRIDQIDANITGWLGSARPRTVLLHIGTNDILQNYNVSSAPNRLSTLIDHIVAAAPAADVFVATIIPLASAGQEAAARTFNAAVPGIVQSKVAGGKRVHLVDMHAALTTADLIDGVHPTATGYDKMAATWYTALRSVSGSIGDTTGTPAAGALVGAASGRCLDVPGSSTTNGTQPVIWDCNGATNQKWTAAGQALQALGKCLDSPTGATAGTKVQLWDCSGATNQRWNRNTNGTISNVASGLCLDVNGNATAAGSPVILWTCTAAANQVWTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W802</accession>
    <name>U5W802_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28925"/>
    <dbReference type="EnsemblBacteria" id="AGZ44046">
        <property type="protein sequence ID" value="AGZ44046"/>
        <property type="gene ID" value="AFR_28925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28925"/>
    <dbReference type="HOGENOM" id="CLU_2630118_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="77" mass="8591" checksum="C5B631AA87E957BD" modified="2014-01-22" version="1">MARERKPHARREDVDRAGPGIVDEDGLAVPQTSRERLPIGAVRHFTTVGDDAELVPVHAPGPTEDTHHMDLRHGRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8A8</accession>
    <name>U5W8A8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29745"/>
    <dbReference type="EnsemblBacteria" id="AGZ44210">
        <property type="protein sequence ID" value="AGZ44210"/>
        <property type="gene ID" value="AFR_29745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6026"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_8_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="15219" checksum="9084AE5EEC0E52AD" modified="2014-01-22" version="1">MISEAAPSDWSALLALQRATHATLQVLAAELVDLDLTASEINALANLADARGLTISELGKAVGVRPTTLTSVLDRLERRGHITRGSRPGDRRVVVVELTSDGRKAATAVRESITRTERRALGGLPVGEMAVLRQALEALAGMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8G4</accession>
    <name>U5W8G4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PASTA domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_30050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30050"/>
    <dbReference type="EnsemblBacteria" id="AGZ44270">
        <property type="protein sequence ID" value="AGZ44270"/>
        <property type="gene ID" value="AFR_30050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6087"/>
    <dbReference type="HOGENOM" id="CLU_1792320_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03793">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00740">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665800" evidence="2">
        <location>
            <begin position="28"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="3">
        <location>
            <begin position="71"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="14614" checksum="ACA083F77BAFCD13" modified="2014-01-22" version="1">MPMHKQRTVRVAVVVLAAMTAFGCSGGSDDKSGGASSQEKPVAAAPVAPAPSRVAPSTFGEGPASAAPAAKAPKLIKLPKVAGENAAIAEDQLRRLGFTNIRLNSSGGGKVGERTAEWTVKKVSPKAGKKLKAGALIVLTCTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8V1</accession>
    <name>U5W8V1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_35955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561783.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35955"/>
    <dbReference type="EnsemblBacteria" id="AGZ45447">
        <property type="protein sequence ID" value="AGZ45447"/>
        <property type="gene ID" value="AFR_35955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7278"/>
    <dbReference type="eggNOG" id="COG0618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039720_0_1_11"/>
    <dbReference type="OrthoDB" id="1040743at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="1">
        <location>
            <begin position="32"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="276"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="36479" checksum="5702772BFC65DE63" modified="2014-01-22" version="1">MTEAVAAPARPAAADWSAAVSTIHELAPTGRVLLICHVNPDGDALGSMLGFALGLCRLGVTQLQATFPGEFEIPEPFTALPGVDLLVPESQAWEDPDLVLVFDVAAESRLGALIDRLPRARATVVLDHHASNTGFGGIQLVDPTAAATSVVAEELLKRLDVPLDVQIAECLYVALATDTGSFRFDMTTPRVHEMAARLIATGLRPGEISRRIFDSRPFGAMKLFADVLGRAQLDPTAAGGHGMVWTYATLDDLERHAQRPYVLDALIDPVRCVAEVDVSVVVKQLSEGVWAVSLRSKGAVDVSAVAIKLGGGGHRLAAGFTGYGRPGDVVTSVRDELEKHLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W909</accession>
    <name>U5W909_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561945.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36765"/>
    <dbReference type="EnsemblBacteria" id="AGZ45609">
        <property type="protein sequence ID" value="AGZ45609"/>
        <property type="gene ID" value="AFR_36765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7437"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_11"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="32580" checksum="1048F27C4CDE1CAD" modified="2014-01-22" version="1">MLDIHRLRLLRELDRRGTLAAVARALSYSPSAISQQLTQLEAETGTTLLEHVGRGVRLTEQARILVRHTEVILERLELAEADLARSMTEVTGTLRVASFQSVMLALVPTALSLVAERHPALRVQLTQAEAHDAFAGLVAHDFDLVLGEEYPGLAQPRLDGVDEEDLTSDEMRFAVPPDGPWSRGELSELAGAPFVFDPGNIIPGQWARNTCRSAGFEPDVRFSSGDLLLQVRLVETGHAVAFLPDLLWAGSSPGAVRLHHLPGRPRRRLFTGVRSGAGQQPRVRAFREALRSSITSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W935</accession>
    <name>U5W935_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_37245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37245"/>
    <dbReference type="EnsemblBacteria" id="AGZ45703">
        <property type="protein sequence ID" value="AGZ45703"/>
        <property type="gene ID" value="AFR_37245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7535"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:cellular aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="7">
        <location>
            <begin position="10"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="active site" evidence="4 5">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="489" mass="51094" checksum="F4B8194AEE9E95F9" modified="2014-01-22" version="1">MPVVEDGFLVSTSPATGAEAGRLPVSDAEAVGATVDRARTAGAWWASLGFDERKVRLLRWRALLAERIEELAELTRAETGKPLADAIVEATAAIEHIDWAARNAKRVLGPRRTKTRLLVAEHSGHLEYQPYGVIGVIGPWNYPILTPLGPISGALAAGNAVVLKPSEYTPAVGQWLADSFAEVVTEQPVFQTVHGLGDVGAALCRSGVGKVAFTGSTATAKKVMAACAETLTPVVIEAGGKDALIVDADADVTAAAEAAVWGAMTNAGQTCIGIERAYAVAPVYDAFVAAVVEKAGKLRLGEEIGPITMPGQVDIIRRHIDDALASGGRAVLGGADAVQPPNVAPTVLVDVPEDSAAIREETFGPTLTITRVADADEAITKANALPYGLGGAVFGKANGIRIARRLHTGMVAVNSTLSFVGMATLPFGGVGDSGFGRIHGEDGLREFGRAKSITVRRAPSLLPAMTFERTPAQVQRIVKAVKLLYGRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W944</accession>
    <name>U5W944_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">3-hydroxybutyryl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_37295</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562048.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37295"/>
    <dbReference type="EnsemblBacteria" id="AGZ45713">
        <property type="protein sequence ID" value="AGZ45713"/>
        <property type="gene ID" value="AFR_37295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7545"/>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_11"/>
    <dbReference type="OrthoDB" id="862072at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="5">
        <location>
            <begin position="5"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="4">
        <location>
            <begin position="186"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="29877" checksum="BA68D60E85B6FD12" modified="2014-01-22" version="1">MAGRLAVIGSGLMGSGIAQVAAQAGWQVTMRDLDEASLSRGVAAIRDSLGRFANKGKISAEDAEAAVGRITTTTELDAMADADVVVEAIYEKLEAKHEVFRALDKICKPGAVLGTNTSAIPITQIAAVTSRPESVVGIHFFSPVPMMKLVELVRGYQTSDETLASARNFAEEVGKTCVEVKRDVAGFVSNRLFSALLVEAIKLVENGVVSAADLDTVMKLGFGHAMGPLATVDLTGLDVMLNAAGNIHRDTGDEKFFPPELLQRMVTAGDLGRKTGKGFYTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9P4</accession>
    <name>U5W9P4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_38370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562263.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38370"/>
    <dbReference type="EnsemblBacteria" id="AGZ45928">
        <property type="protein sequence ID" value="AGZ45928"/>
        <property type="gene ID" value="AFR_38370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7762"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="154"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23443" checksum="65070F3ADF063419" modified="2014-01-22" version="1">MIDLLVVDDNPVVRAAVHGFLAGQDEIRVVGEAADGREALRLAHRLRPAVTLLDYRMPVADGLSVIATLAEHTRVLVLTSDTGAEIVAGMLRGGARGYLVWGQFEPPDLLRAIREVAAGSSWLSPVAASVAATAFREQGARERAAREQEARQASARQRHGISAREEEVLDLLGLGLSNASIAHRLGLTEKTVKNHLNHIFAKLGVSNRTEAVVRWRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9R1</accession>
    <name>U5W9R1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_31955</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560984.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31955"/>
    <dbReference type="EnsemblBacteria" id="AGZ44651">
        <property type="protein sequence ID" value="AGZ44651"/>
        <property type="gene ID" value="AFR_31955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6468"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_11"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="8"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="335" mass="36368" checksum="4B24CD28FE88FE7C" modified="2014-01-22" version="1">MSEPVLEIKNLNVDYGLGDQAVHAVRDVNLTLHRGEVLGLAGESGSGKSTLAYGLTRLLAPPGVVSGGQVIYHPEKGDAYDVLGLSDKELQAFRWAETAIVFQGAMNSLNPVHKISTQLTDVLAAHEPQMSSHSRTARAREMLKLVGISPDRMDAYPHQLSGGMRQRVMIGMALILQPQVVIMDEPTTALDVVMQRQILGQLIELRERLGFSVIFITHDLSLLVEFSNRIAIMYGGRIVEEAQASTLYRESLHPYSAGLLGSFPALRGPRRELTGIPGSPPDLKGMPTGCSFHPRCPKAFDPCADNIPVLGNPDTPDGATRTVACWLHPVKQPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9S1</accession>
    <name>U5W9S1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="1">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>9-ribosyl-trans-zeatin 5'-phosphate + H2O = D-ribose 5-phosphate + trans-zeatin</text>
            <dbReference type="Rhea" id="RHEA:48564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16522"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:87947"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N(6)-(dimethylallyl)adenosine 5'-phosphate = D-ribose 5-phosphate + N(6)-dimethylallyladenine</text>
            <dbReference type="Rhea" id="RHEA:48560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="ChEBI" id="CHEBI:57526"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562287.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38495"/>
    <dbReference type="EnsemblBacteria" id="AGZ45953">
        <property type="protein sequence ID" value="AGZ45953"/>
        <property type="gene ID" value="AFR_38495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7788"/>
    <dbReference type="eggNOG" id="COG1611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058336_4_0_11"/>
    <dbReference type="OrthoDB" id="1255127at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102682">
        <property type="term" value="F:N6-(Delta2-isopentenyl)-adenosine 5'-monophosphate phosphoribohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00730">
        <property type="entry name" value="TIGR00730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="179" mass="19018" checksum="07F00D7EB36AC2D7" modified="2014-01-22" version="1">MAAICVFCASSSTLDQQWLDLATQLGKALGPRGHTLVSGGGRVGMMGAVADGARAGGAHTLGVIPQSLVDLEVADTAADELIVTTDMGLRKNLMIERSDAFITLPGGLGTLDELFEVWTTATLDLHRKPIIVLDPNGFYDGLLAWLDRLAETEFVRRPALAKITTVTSIEAALDLIERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA21</accession>
    <name>U5WA21_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycoside hydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_32590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561115.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32590"/>
    <dbReference type="EnsemblBacteria" id="AGZ44776">
        <property type="protein sequence ID" value="AGZ44776"/>
        <property type="gene ID" value="AFR_32590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6595"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019533_4_1_11"/>
    <dbReference type="OrthoDB" id="1046649at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="GH16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665847" evidence="1">
        <location>
            <begin position="44"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="3">
        <location>
            <begin position="51"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="323"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="452" mass="49044" checksum="21B73DE06871125C" modified="2014-01-22" version="1">MTLTPGFQGRTLSRSRRRLSVLLIAVVTALVGSAVIQQQPAQAAWNLVWADEFNGSGAPSSANWNYNVGNGLNPGLNSFDGWGNGEWEWYRPENCTQSGGNLVMRANWLTTPITVNGRNFYQTSCRMTTDTKRSFQYGRIEARMALPTATGSWPAFWMLGDSCDESSTSAYNPAQTYHDRLPTNWASCGEVDIMEHANANATVTNNIFWDLRTGVFPWTAGQNANYVANPAVNNAAAFHIYAIEWTAAQIRWYVDGVQTHVIDTTPATLEEFRKPFHIIFNLALGGTYPGQNPVQSQFPLTASIDYVRYYQDGGGTTPPPPTGGSATQINGPSGKCVDVAGDNTGGNGAAVQLWDCQGTAATDQQWTWNGQTLRTLGRCLDVQSGSTANGAKLQLWDCNGGGAQNWVQEGNRLRNPQSNRCIDSPSGATANGTRLQIWDCNGTAAQSFVKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA26</accession>
    <name>U5WA26_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative AdpA-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561172.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32885"/>
    <dbReference type="EnsemblBacteria" id="AGZ44835">
        <property type="protein sequence ID" value="AGZ44835"/>
        <property type="gene ID" value="AFR_32885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6655"/>
    <dbReference type="eggNOG" id="COG4977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_59_0_11"/>
    <dbReference type="OrthoDB" id="871215at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="215"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="32710" checksum="9FC6292C22DEC6B8" modified="2014-01-22" version="1">MSHVVFLLVPQLHLLDLAGAAQVFSTAADHGYGHRLTYVAEQPDVPTHQGITLQAGTQWPELTADDLIVVPGWRSPQLAGTGHLEATTLQRLAGHHAAGGTVASVCAGADALGRAGLLDGRRCTTHHDVQDDLARRYPRASVVRDVLYVEDDRVITSAGIASGIDLALHLIAVRHGPAAAAQVARQMVVYARRNGDEQQFSAMLRHRAHLSDVVHRVQDRIDAGFTEPLPLTELARAAGVSERTLTRLFESATGQTPLRYQQLLRVERAEHLIGHGSTMESAARAVGFGDARMLRRLRARTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA76</accession>
    <name>U5WA76_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Signal transduction histidine kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561227.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33160"/>
    <dbReference type="EnsemblBacteria" id="AGZ44890">
        <property type="protein sequence ID" value="AGZ44890"/>
        <property type="gene ID" value="AFR_33160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6710"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="168"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="38799" checksum="790388F56C63975D" modified="2014-01-22" version="1">MDQRAHPALRYAAALVVMAFFGATLITVVSRDPGRLGEALLDAGLGFAGLGLLHWRHRRPFAVALVIAVFAAASTTAFGPAFVAFVSLTTHRRFRQIAVVGATLWVGNAVSAFWRDGFSQVTSVGIAAMTLSLVTLTITGLYVAGRRDLAEAEARRDEERLEQARLGERVRIAREMHDVLAHRLSLLSLHAGALAHRTDLTPAEVRAAAAVIQENAHQSLDELRATLGVLRAETAPQPSFDTLGTLFTEVRAAGQQVTVTDTVRSREVLPVQAGRHAYRIVQEGLTNARKHAPGAPVTVALDGGPGADLRVTISNPAPASADTGEGVGLVGLSERMAMIGGSLSRRHDGGRFVLDATLPWEVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAG1</accession>
    <name>U5WAG1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fructose-1,6-bisphosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glpX</name>
        <name evidence="7" type="ORF">AFR_39225</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the FBPase class 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562433.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39225"/>
    <dbReference type="EnsemblBacteria" id="AGZ46099">
        <property type="protein sequence ID" value="AGZ46099"/>
        <property type="gene ID" value="AFR_39225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7937"/>
    <dbReference type="eggNOG" id="COG1494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054938_0_0_11"/>
    <dbReference type="OrthoDB" id="879338at2"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01516">
        <property type="entry name" value="FBPase_glpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004464">
        <property type="entry name" value="FBPase_class-2/SBPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30447">
        <property type="entry name" value="PTHR30447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03320">
        <property type="entry name" value="FBPase_glpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004532">
        <property type="entry name" value="GlpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00330">
        <property type="entry name" value="glpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="174"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="196"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR004532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR004532-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004532-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="341" mass="35779" checksum="1EDD397231E185C3" modified="2014-01-22" version="1">MPASQNPQDLDRNIALDLVRVTEAAAMAAGRWVGRGDKEGGDGAAVDAMRKLINSIQMRGVVVIGEGEKDNAPMLFNGEEVGDGTGPEVDVAVDPVDGTTLMSKGMPGAVAVLAVAERGAMFDPSAVFYMEKIAVGPDCADVIDINAGATENLRRIAKAKRSSVSDVTVCILDRPRHQSLIDEVRAAGANIKFISDGDIAGAISAARTESDVDVLMGIGGTPEGITAACALKCLGGAIQAKLWPLDESEREKAIGGGHDLDRVLSTDDLVTGDNCFFVATGVTSGDLLKGVRYRAGGAHTQSIVMRSKSGTIRVIDSYHRLEKLALYSSVDFDGHLPTVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAH4</accession>
    <name>U5WAH4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative GtrA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_39300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39300"/>
    <dbReference type="EnsemblBacteria" id="AGZ46114">
        <property type="protein sequence ID" value="AGZ46114"/>
        <property type="gene ID" value="AFR_39300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7951"/>
    <dbReference type="eggNOG" id="COG2246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083873_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA" evidence="8">
        <location>
            <begin position="31"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="21215" checksum="23A11865B35EE353" modified="2014-01-22" version="1">MTRAGTLGIVPAEILPALRARFGALVRELSKFGTVGSIAFAIDLAIFNVLLQTGSETLIAKTISTVIATTVAFLGNRFWTWRHRGHAHMARQYTMFFLLNAVGLGIGLACLAISHYGLGQVWPEFQTPLADNISGQFVGTAVGTLFRFWSYRRFVFGDTVVPDPIEPGLPVQSHLHASAPAPQSAPPSTPRAVPRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAI8</accession>
    <name>U5WAI8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45005">
        <property type="protein sequence ID" value="AGZ45005"/>
        <property type="gene ID" value="AFR_33735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6828"/>
    <dbReference type="HOGENOM" id="CLU_123724_0_0_11"/>
    <dbReference type="OrthoDB" id="2076339at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="159" mass="17810" checksum="A6E4D5D30EC9D317" modified="2014-01-22" version="1">MINVVSLLVALIAVIVSSTLTWRALTIARNANHFPVIVSLLAPHRRPDFIRKERHLVDHLGDHDPELTLEGLPEPIRTYAIEVSEQYHMLGYVAANGLADGTLLTTQVGHGASRVWDAVQPFVAVERRNRGGEYSFFNSFELFVELARKIDTSVPDARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAQ6</accession>
    <name>U5WAQ6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GGACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562406.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39090"/>
    <dbReference type="EnsemblBacteria" id="AGZ46072">
        <property type="protein sequence ID" value="AGZ46072"/>
        <property type="gene ID" value="AFR_39090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7911"/>
    <dbReference type="eggNOG" id="COG2105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152489_0_0_11"/>
    <dbReference type="OrthoDB" id="1984556at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="SSF110857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGACT" evidence="1">
        <location>
            <begin position="5"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="114" mass="12053" checksum="BFBF4F27997B4175" modified="2014-01-22" version="1">MSELLFSYGTLRQSGVQQALFGRAVPTVADVLPGYRVDVVVITDPEVIAQSGSDRHPILRRGADGDAVDGACLELTPDELAAADAYEVDDYVRVAVTLGSGRNAWVYVAGEDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAY6</accession>
    <name>U5WAY6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PfkB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34385</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561472.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34385"/>
    <dbReference type="EnsemblBacteria" id="AGZ45135">
        <property type="protein sequence ID" value="AGZ45135"/>
        <property type="gene ID" value="AFR_34385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6957"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_12_0_11"/>
    <dbReference type="OrthoDB" id="1604782at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="3">
        <location>
            <begin position="180"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="31279" checksum="634EE524AC9F197A" modified="2014-01-22" version="1">MTVLVVGGTGIDTVVPVGELPILAADGVQAAGPVRTFVSHTGTCVALGLRNLGVEVTVADAVGDDEEGRRITAAFAGWGIPLRTAISTGGTRRAVNLMDPHGRRLSIFDARVVPGYRLPVETYRPLLDRARHVHVTLTGWARHLLAEARTGGRTVSTDLHDWNGTDDYHRDFALGADLVFVSGVQLGDRAAEVARWILADGVASHVVVTAGAAGADLYTAGGHLHEPAADLYTAGGHLHEPAADPGGPIVDSNGAGDALAAAFLAALSDGLDDAACLRRGAIAGAYACTRPVGPDGFIRRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB43</accession>
    <name>U5WB43_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34695</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561532.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34695"/>
    <dbReference type="EnsemblBacteria" id="AGZ45195">
        <property type="protein sequence ID" value="AGZ45195"/>
        <property type="gene ID" value="AFR_34695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7020"/>
    <dbReference type="eggNOG" id="COG3979">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058767_0_0_11"/>
    <dbReference type="OrthoDB" id="651936at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036573">
        <property type="entry name" value="CBM_sf_5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="GH16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51055">
        <property type="entry name" value="SSF51055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665743" evidence="2">
        <location>
            <begin position="33"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="3">
        <location>
            <begin position="22"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="396" mass="42691" checksum="600131D120CC5393" modified="2014-01-22" version="1">MSIDKRKSLGFVGTLALLGTVAVFTNAPSAAAAECGYLFDDFSYSSSSDSSLTAHGWTPRSYAGGPGVPGATWSPNSITFPTADGQKVMQLQASTDGTGAGTNHAELYSTQKRYFEGTYASRVRFTDAPVSGNDGDHINQTFFTISPLNGDLDPTYSELDISEYLPNGGWGETGPINYQTTWYTYRAEPWFADNQHSEQRSSLNGWHDLVATVANGHVIYYIDGVQVGDHSGKFYPRQTMTINWNLWFIDTATHSGGLSTYIQQVDWVLFAKNQALTPAQVSSKTAGYRSSGSTFADTVDTTGGGCTNPTNPPTTPPTTPPTTPPTTPPAGSCATAPEWAFGSVYQGGQTVKHEKSKYGDPSGPASGDGKHLWKARYWTQGSEPGWTQQWEDLGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBH8</accession>
    <name>U5WBH8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycine betaine/carnitine/choline-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_41420</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562870.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41420"/>
    <dbReference type="EnsemblBacteria" id="AGZ46538">
        <property type="protein sequence ID" value="AGZ46538"/>
        <property type="gene ID" value="AFR_41420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8381"/>
    <dbReference type="eggNOG" id="COG1732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_731353_0_0_11"/>
    <dbReference type="OrthoDB" id="770506at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007210">
        <property type="entry name" value="ABC_Gly_betaine_transp_sub-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04069">
        <property type="entry name" value="OpuAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666262" evidence="2">
        <location>
            <begin position="21"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="OpuAC" evidence="3">
        <location>
            <begin position="49"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="OpuAC" evidence="3">
        <location>
            <begin position="133"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="333" mass="33805" checksum="185702634604BA4C" modified="2014-01-22" version="1">MRRHLLLTAGTLMTAAVILAGCGDSGSSGTEAPASNAAGSAGCAPVAGDKLVVLTDDKNLQNTDNIVPAINKKASSPELIAALDKVSAALDTTKLIELNKAVDIDRKSSKAAAEEFATANSITSGITKGPGGDITVGAANFSENATLGELYNIALTAAGYKVKVQQIGNRELYEPALEKGDIDVVPEYAATLATFLSGKIDGKDAKDPSSPQIDTTMTNLTALGEKAGLVFGKPSAAQDQNAFATTEAFTTKYGVKTLSDLAAKCSGAATVLGGPPECPQRPKCQQGLVETYSFQAGKFSSLDAGGPLTKTGLKQGTISVGLVFSSDGALAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBJ0</accession>
    <name>U5WBJ0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">LexA repressor</fullName>
            <ecNumber evidence="12">3.4.21.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">lexA</name>
        <name evidence="17" type="ORF">AFR_35520</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolysis of Ala-|-Gly bond in repressor LexA.</text>
            <dbReference type="EC" id="3.4.21.88"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12 13">Belongs to the peptidase S24 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.88" evidence="12"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35520"/>
    <dbReference type="MEROPS" id="S24.001"/>
    <dbReference type="EnsemblBacteria" id="AGZ45360">
        <property type="protein sequence ID" value="AGZ45360"/>
        <property type="gene ID" value="AFR_35520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7189"/>
    <dbReference type="eggNOG" id="COG1974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_45_0_11"/>
    <dbReference type="OrthoDB" id="1933795at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06529">
        <property type="entry name" value="S24_LexA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00015">
        <property type="entry name" value="LexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006200">
        <property type="entry name" value="LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039418">
        <property type="entry name" value="LexA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006199">
        <property type="entry name" value="LexA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006197">
        <property type="entry name" value="Peptidase_S24_LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01726">
        <property type="entry name" value="LexA_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00726">
        <property type="entry name" value="LEXASERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00498">
        <property type="entry name" value="lexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="11 12" id="KW-0742">SOS response</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="LexA_DNA_bind" evidence="16">
        <location>
            <begin position="59"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S24" evidence="15">
        <location>
            <begin position="147"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="12">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="12">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="12">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="12">
        <location>
            <begin position="154"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ45360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="29309" checksum="CE75FF5E60A62F20" modified="2014-01-22" version="1">MSTDDRTSRQHQKELQPEAANTKAAMRRRTPSRARSGDAPQLRAVTPVVSQFPDLVTADLTARQRRILEFIRDWVERYGYPPSVREIGEAVGLVSPSSVAYQLKELEKKGFLRRDPNRPRAVDVRAPSEMVDDETLRSARPTPAYVPLLGRIAAGGPILAEQAVEEFFPLPRELVGEGDVFMLEVKGDSMIDAAICNGDWVVVRQQPTANVGDIVAAMIDGEATVKSYRQRDGHVWLMPANPAFDPIPGDDATVMGRVVAVLRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC95</accession>
    <name>U5WC95_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_36425</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561877.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36425"/>
    <dbReference type="EnsemblBacteria" id="AGZ45541">
        <property type="protein sequence ID" value="AGZ45541"/>
        <property type="gene ID" value="AFR_36425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7371"/>
    <dbReference type="eggNOG" id="COG0004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_0_11"/>
    <dbReference type="OrthoDB" id="562287at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="PTHR43029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium_transp" evidence="9">
        <location>
            <begin position="9"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="451" mass="46750" checksum="44A70354834AEF71" modified="2014-01-22" version="1">MEINTGNTAWLLLSSALVLLMTPGLALFYGGLNRSKGALNMMMMSFSSIGLVSVLWVLYGFTLAFGTNDSSGTNHVIGSFSQYFGTGTTWIGEEWMVAGAGTGIPTYVFMVFQMMFAIITVALISGALSDRLKFGGWILFAFGWFTLVYVPVAHWVWGGGWIGVNLGALDFAGGTAVHINAGAAALGLILVLGKRVGWPREAMKPHNVPFVALGAGLLWFGWFGFNAGSELTVDSITAVAFVNTQVATAAALLGWIIVEWLRDGKPTLVGASSGAVAGLVAITPACGFIAPLPAAVLGIIAGAVCALAVGLKYKLGYDDSLDVVGVHFVGGWIGSLGIGFFATTQVNAVAARESLFYGGGVDQLWRQFVGSAAVTVYSLVIAVVLALILKAVKVLRVDGETEVSGIDVAEHAESAYDFSPTTGSGSAFAMAGITPAGTTDGKSSVSEKVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCS1</accession>
    <name>U5WCS1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA synthetase</fullName>
            <shortName evidence="7">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">serS</name>
        <name evidence="9" type="ORF">AFR_42590</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563103.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42590"/>
    <dbReference type="EnsemblBacteria" id="AGZ46772">
        <property type="protein sequence ID" value="AGZ46772"/>
        <property type="gene ID" value="AFR_42590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8619"/>
    <dbReference type="eggNOG" id="COG0172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_1_11"/>
    <dbReference type="OrthoDB" id="353391at2"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="PTHR11778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="135"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Serine binding" evidence="7">
        <location>
            <begin position="226"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="257"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="344"/>
            <end position="347"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="420" mass="46349" checksum="E0BC62A1E3F23335" modified="2014-01-22" version="1">MIDLRLLRENPDLFRASQELRGESASKVDDLLRADEERRATTQRFESVRAEQRSLGSLVAKAKGDERTALLARTKELSAAVKEAQAATTEADQALRQAHLAVPNLVQDGVPPGGEDDFVVLREVGDRPEIDNPKDHLEIGEALRAIDTERGAKVSGSRFYFLTGVGALLQLGMLQMAIAQAVEHGFIPSITPALVKPEAMEGTGFLGAHASEIYRLEADDLYLVGTSEVALAAYHSNEILDLANGPERFAGWSSCFRREAGSHGRDVRGILRVHQFDKVEMFSFCRPEEAADEHLRLLAMEEEMLAKVEVPYRVIDVAAGDLGSSATRKFDCEAWVPSQGRYREVTSTSNCTTFQARRLNIRYRDEDGKAQTAATLNGTLATTRWLIPILENHQQPDGSVRVPKALQPYLGGRDVLEPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCV7</accession>
    <name>U5WCV7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42765"/>
    <dbReference type="EnsemblBacteria" id="AGZ46807">
        <property type="protein sequence ID" value="AGZ46807"/>
        <property type="gene ID" value="AFR_42765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8655"/>
    <dbReference type="eggNOG" id="COG0718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144751_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="15473" checksum="2B9D9D5DB1CC452F" modified="2014-01-22" version="1">MADRDANHALRARFDDVYGQYNRLRSGMDDVQRRLAELRASADSDDGLIRATVGPRGQLLDLRLDRRIYRDMDAAELSRKIVTTVEQATAKATQQVEQLMADYLPADSGPMQFLKDNNFGSLLRRQDKIMREAGGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD29</accession>
    <name>U5WD29_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATPase/permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_37985</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562186.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37985"/>
    <dbReference type="EnsemblBacteria" id="AGZ45851">
        <property type="protein sequence ID" value="AGZ45851"/>
        <property type="gene ID" value="AFR_37985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7682"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="24"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="342"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="376"/>
            <end position="383"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="595" mass="64501" checksum="402FEBE9AC60F115" modified="2014-01-22" version="1">MRDLWLGITGVLGSSWRASKGRLLISAFLMLLGNVSAPLIAVFLALATDATLERDTDLAVWAAVAVALVALLSLTMEHFAHIFFFELADLHHLSVEREIGDLAHGTAGLDQHERQDYADRMELLRKESWEIANGVQTVLTAITLSAQIVLTAVLLARVEPWLLLLPLFAVPPLIAGRFAEARLERAELKTAEPRRLGWHLLELAVSAAAAKEIRLFGLQRVLRERQAAARRAEEVPLRRAEVAGLGLRLIGQLIFAIGYVAAVVVVVRSAIGGERSVGDVVLVITLAVQTNAQAAGVVAVAGALQRNARALARVRWLRRETELAADPIVADQPVPSALHDGIRFDGVAFRYPGTETDVLRDVNVHIPAGATVAIVGDNGAGKSTLVKLLSRFYRPSAGEITVDGVDLRRISPDRWRERIAAGFQDFVKLEAPARESIGVGDLARSDDAEAVRAAVHRADAELVIERLPEGLESHLGKAYEDGEELSGGQWQRVALSRAMMRDEPLLLLLDEPTAALDPLTEHALFERYAASAREVGSRTGGITVLVSHRFSTVRMADLILVVADGGIAEAGTHQDLMARNGLYAELFTLQAAAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDJ2</accession>
    <name>U5WDJ2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">bPH_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562389.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39005"/>
    <dbReference type="EnsemblBacteria" id="AGZ46055">
        <property type="protein sequence ID" value="AGZ46055"/>
        <property type="gene ID" value="AFR_39005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7894"/>
    <dbReference type="eggNOG" id="ENOG503304E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143522_0_0_11"/>
    <dbReference type="OrthoDB" id="1682253at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012544">
        <property type="entry name" value="PHb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037063">
        <property type="entry name" value="PHb_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08000">
        <property type="entry name" value="bPH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="bPH_1" evidence="1">
        <location>
            <begin position="14"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="12903" checksum="30E41AE869DBB071" modified="2014-01-22" version="1">MTAPVYDSKGQLQQIESGLLEGETIIAVYDAVGAGTGFIGLTTRRVIIQDKSFAGKRVAITSIPYAKVSSVSVVSNKSWAGQFFSTGTIAITVGTHTYEVEFRGSEKTHHVHNVILHYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEW2</accession>
    <name>U5WEW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transport system inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_41430</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562872.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41430"/>
    <dbReference type="EnsemblBacteria" id="AGZ46540">
        <property type="protein sequence ID" value="AGZ46540"/>
        <property type="gene ID" value="AFR_41430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8383"/>
    <dbReference type="eggNOG" id="COG1174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_7_2_11"/>
    <dbReference type="OrthoDB" id="1233189at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="43"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="240" mass="25345" checksum="37D4E2E45E116600" modified="2014-01-22" version="1">MSFLSRAPAPAWPLAAPADDGPGNPWFSWRYVHDNASEILTALQFHAGLTARAVIIALIVALPLAVVSYWWRPLTGPILALSGVLYTIPSLALLALLAPAVGTTSDTSVLIALVLYALLVLVRNSLAGLTQVPGEVRDAAAGMGYGRFGRLWRIELPLALPGILTGLRLATVSTVALVTVGSLIGKGGLGEMILGGFRNNFYKAEIMTGTLLCVALALVLDLLLAGVGRLLTPWTRERKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF44</accession>
    <name>U5WF44_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562981.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41980"/>
    <dbReference type="EnsemblBacteria" id="AGZ46650">
        <property type="protein sequence ID" value="AGZ46650"/>
        <property type="gene ID" value="AFR_41980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8494"/>
    <dbReference type="eggNOG" id="ENOG50315GV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086035_1_1_11"/>
    <dbReference type="OrthoDB" id="1983827at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010840">
        <property type="entry name" value="YqiJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07290">
        <property type="entry name" value="DUF1449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="20007" checksum="46A27644892D1EF6" modified="2014-01-22" version="1">MGGFFEAALSFPAVIFTPLLVVVIGYWLVVIVGGADPDGDGDGGDAGGFLGFLGLGGVPASVVLSLLIAVAWFAALAGGELLGAIPAALVLAGALVAAWVITRLGVMLLRRLMPQGSEPSRADFVGLTGIIRTGRVTATFGQAEVHSRDGSSAIVQVRQAGTDDLRAGSIALLYDVDPDGEFFWVVPADIATSPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFJ4</accession>
    <name>U5WFJ4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Flavohemoprotein</fullName>
            <ecNumber evidence="6">1.14.12.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Flavohemoglobin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Hemoglobin-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Nitric oxide dioxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AFR_42185</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADH + 2 nitric oxide + 2 O2 = H(+) + NAD(+) + 2 nitrate</text>
            <dbReference type="Rhea" id="RHEA:19469"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate</text>
            <dbReference type="Rhea" id="RHEA:19465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the globin family. Two-domain flavohemoproteins subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.14.12.17" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563022.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42185"/>
    <dbReference type="EnsemblBacteria" id="AGZ46691">
        <property type="protein sequence ID" value="AGZ46691"/>
        <property type="gene ID" value="AFR_42185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8537"/>
    <dbReference type="eggNOG" id="COG1017">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026437_2_1_11"/>
    <dbReference type="OrthoDB" id="1834577at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051409">
        <property type="term" value="P:response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023950">
        <property type="entry name" value="Hmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF3">
        <property type="entry name" value="PTHR43396:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="SSF46458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="17" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GLOBIN" evidence="18">
        <location>
            <begin position="8"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="19">
        <location>
            <begin position="138"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AGZ46691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="368" mass="41874" checksum="23948765CCC92346" modified="2014-01-22" version="1">MGDLARLLKESWSLVEEHQDKVAGYFYARIFLSHPDLRDLFPVHMDVQRSRLLNAIITAVQTLEDPEKFDDYLRALGRDHRKFHVEPEHYEVVGGALIEAMRQFAGEHWGIEYDQAWADAYAVIASKMLAGAEADTNPPYWYAEVTSHERRANDIAVFTVTPMQPLEFRAGQYLSVECPRFQPRLWRTYSPANAPRRDNTLEFHVRAVGAGWVSSALVRRLEVGDMIRLAAPMGSMNLDRRSTRDAVFVAGGTGLAPIKSLLEELTRYNRTRWVHVFFGARTRDDLYDLAELNRLAARYPWLSVVTACSDDPTFPGEQGNISDVVARYGPWNEHDFFVSGSGPMVKATMRTLAELQVPSVRIKYDSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VN22</accession>
    <name>U5VN22_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NmrA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357322.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00445"/>
    <dbReference type="EnsemblBacteria" id="AGZ38378">
        <property type="protein sequence ID" value="AGZ38378"/>
        <property type="gene ID" value="AFR_00445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.91"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_10_4_11"/>
    <dbReference type="OrthoDB" id="1616572at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="29471" checksum="CD6DAB1A75B73A4A" modified="2014-01-22" version="1">MTIVVTGATGHFGRVAVESLLSRGVPADQIVAIGRSVEKIQDLADRGVRVIHASYEDPESLRKAFAGADKLLFVSGSEVGKRLEQHANVVAAAKDAGISKVVYTSAPYADTSDMMIAVEHRATEQALAGSGIPDVHFLRNSWYLENYNVKGALEHGLFGAAGEGKISIATRADLAEAAAAAVVADNLDKQAYELGGEAVTLTELAAEVSRQSGQEVGYTNLPQEKYQEFLVGVGLPEQFAAVLADSDRGASVGLLDTGAGDLAQLLGRPATPLADAVRAELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNQ9</accession>
    <name>U5VNQ9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="5">ATase</shortName>
            <ecNumber evidence="5">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="5">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purF</name>
        <name evidence="12" type="ORF">AFR_00740</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5 8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5 9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357381.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00740"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="AGZ38437">
        <property type="protein sequence ID" value="AGZ38437"/>
        <property type="gene ID" value="AFR_00740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.149"/>
    <dbReference type="eggNOG" id="COG0034">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_2_11"/>
    <dbReference type="OrthoDB" id="267682at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 6 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="23"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="493"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5 7">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ38437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="536" mass="57532" checksum="4FD2A36F23F7596E" modified="2014-01-22" version="1">MPRGDGRLTDDLDPQDRGPQDACGVFGVWAPEEEVAKLTYFGLFALQHRGQEAAGIAVSDGSGVVVYKDLGLVSQVFDEPTLASLRGHLAIGHTRYSTTGGSNWENAQPTIRATTAGTTIALAHNGNLVNTAELAREVAERGLEADMGTSDTALVTTLLASRPDLSVEAAALEVLPRLRGAFSFVFMDESTLYAARDAQGVRPLVLGRMERGWAVASETAALDIVGASFVREVEPGEIIAIDEHGLRSTRFAAPEPKGCLFEYVYLARPDTTIAGRNIYSARVEVGRKLAKEHPVEADLVIGVPESGIPAAIGYAEASGIPYSAGFMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVVRGKRIVVIDDSIVRGNTQRAQIRMLREAGALEVHVRISSPPVKWPCFYGIDFATRAELVANGLEIDGIRRSIGADSLGYVSLDGLVQATEQPKTRLCMACFDGQYPIELPAGHLIGKHLLESVGKRAAMPDASSQATSAAVAGLEQDYEDEQYTEGERETAKPLVGSPGGVDALRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNR5</accession>
    <name>U5VNR5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_00765</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357386.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00765"/>
    <dbReference type="EnsemblBacteria" id="AGZ38442">
        <property type="protein sequence ID" value="AGZ38442"/>
        <property type="gene ID" value="AFR_00765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.154"/>
    <dbReference type="eggNOG" id="COG0334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_0_0_11"/>
    <dbReference type="OrthoDB" id="1184827at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016211">
        <property type="entry name" value="Glu/Phe/Leu/Val_DH_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42722">
        <property type="entry name" value="PTHR42722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000188">
        <property type="entry name" value="Phe_leu_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="6">
        <location>
            <begin position="147"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="183"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000188-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000188-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="360" mass="38209" checksum="7A1206871951CC65" modified="2014-01-22" version="1">MGVFVSSDGTDAAGHEQVVFCQDRHTGLKAIIGIYSTALGPALGGTRFYPYESEEAALTDVLNLSRGMAYKNALAGLDLGGGKAVIWGDPGQVKSEALLRAYGRFVESLNGRYYTACDVGTYVPDMDVIARETRYVTGRSLEHGGAGDSSILTAWGVFQGMRAAAEHTWGSPTLAGRRVGVAGLGKVGKYLAGHLIEDGASVVATDVNEAALEWARTTYPQIDLVADTQTLITSDIDVYAPCALGGALDDDTVPALRAKVVTGGANNQLAHPGIEKLLEERGILYTPDYVVNAGGVIQVADEIEGFNFERAKLRATRIFDTTGQILRLADDEGVPPAVAADRLAERRMAEVGRLRSIFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPC8</accession>
    <name>U5VPC8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Immune inhibitor A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_01800</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01800"/>
    <dbReference type="EnsemblBacteria" id="AGZ38649">
        <property type="protein sequence ID" value="AGZ38649"/>
        <property type="gene ID" value="AFR_01800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.364"/>
    <dbReference type="eggNOG" id="COG4412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010858_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012300">
        <property type="entry name" value="Pept_M6_InhA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008757">
        <property type="entry name" value="Peptidase_M6-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05547">
        <property type="entry name" value="Peptidase_M6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007519">
        <property type="entry name" value="Protease_InhA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03296">
        <property type="entry name" value="M6dom_TIGR03296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665930" evidence="2">
        <location>
            <begin position="27"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M6" evidence="3">
        <location>
            <begin position="91"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="782" mass="84767" checksum="899FB46C55B50FBD" modified="2014-01-22" version="1">MVSSVGIMLPAAAMAAPPVDPAPAQASKKAPVDDLPNPFEDKRRELRQEAVNGILKGTVKTQKRGGSTVAKVGKTRGGGETNRKVAKAGQDQYVEVGREQTDRIFVILAEFGNDRHPNYPDVDSDPATAGPTTFEGPLHNAIPSPDRTKDNSTVWQSDYNQAHYQDLYFGEGKNAESLKTYYETQSSGRYSVDGEVSDWVKVKYNEARYGRDCDITGLCTDTNMWALVTDAANQWVADQKASGRTDAQVKADMASFDQWDRYDFDGDGNFNEPDGYIDHFQIVHAGGDQSDGDPQQGEDAIWAHRWYAYNTSAGVTGPATNKAGGTQIGNTGIWIGDYTVQPENGGLSVFAHEYGHDLGLPDDYDTSGGSNNNSEFWTLMAQSRLSGAGEPLGTRPGDLGAWNKMQLGWLDYETVVAGQKKTLNLGPQEYNTKKAQAAVVVLPDKEVTTDLGAPFAGESQYFSGNDDNLNTTLTKSVDLTAATSASFDLKGRYNIEEGYDYLYFEGSTDGTTWTALNGTVDGAPLGVDGAVPPRPALGGDSGGAWKDISIPLTQYAGKKVQVRLHYITDGGLSAGGFFGDDLTITANGAVVSTDGAEGTPEWTAAGFSVEGSSITAEYDNFYIAGNRTYTSYDKYLKTGPYNFGWATTKPDWVEHFAYQQGLLISYNDTSFADNNVNVHPGGGRNLIIDSHPAPFYNLDGVPWRSRIQLYDAPFGTTKADSFTLHINGKPSYIRGQAAQPTFDDTKKYFYDEIPWAGVKLPAVGVKIKVVEQNGTSLKVKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPJ0</accession>
    <name>U5VPJ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_02150</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02150"/>
    <dbReference type="EnsemblBacteria" id="AGZ38719">
        <property type="protein sequence ID" value="AGZ38719"/>
        <property type="gene ID" value="AFR_02150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.435"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="22648" checksum="203DFA2905594251" modified="2014-01-22" version="1">MIARLRQQITSGAWPVGSRIPTEPELVTQLGVARNTVREAIRALAHNGLLDIRQGSGTWVIATSELAGVMQRRFATADPDHITELRGTLEAAAAGFAARRRTTDDLERLDMLLGQREQAWATGVRDAFVDADTAFHSAVVAASHNDALIALYADLGQVIRESLRGHFGAELRPEEYMDHTRLVDAIRAQDQGVATAEAAAHTSSCEAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPU4</accession>
    <name>U5VPU4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357767.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02675"/>
    <dbReference type="EnsemblBacteria" id="AGZ38824">
        <property type="protein sequence ID" value="AGZ38824"/>
        <property type="gene ID" value="AFR_02675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.538"/>
    <dbReference type="eggNOG" id="ENOG50333EE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_569421_0_0_11"/>
    <dbReference type="OrthoDB" id="1061345at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="469" mass="49057" checksum="E08F5ABE1332FA35" modified="2014-01-22" version="1">MKLIGGEMAGFSDREPVGGLLLTELIGLLRPLGDRVLVAGPHAPELITALAGFAEVTCLVRGEPDAAALTGSGVTVLCGSLAKLTDTEQYDVVVALDGVDRLCSVEGPQYDWAESVQVLRRALRPGGALLLSVENELGVHRLVDRVTVTFAHTTDAWRPLGEFGTKPGNPARLEAGLTAEGLSIGWLGAAWPLPSTPTFAATPAGLREGAAGALATAAVASAFAGRPVLSDPRRLTAAAVRAGLGAELAPSWLVLAYRGPAGSLVLPQVLGHAAGNLPEGRLLEELLIGACLRHDLPAIRGLLSGWAAAVPTASFDNVLVDGDTFAVLDPTAADGTPAEALQRFAHTLLAGGYAQPWPGAADVPSLTTILLAAAGLDPDSPVPTAVPVVPDSLREHEEHVRRLEEQLADAAERIAYVETELVLRDAELRRARLQIDAFSGRAVYRITKFSARAARKAVRAARRSPLFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQK1</accession>
    <name>U5VQK1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_03840</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358002.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03840"/>
    <dbReference type="EnsemblBacteria" id="AGZ39057">
        <property type="protein sequence ID" value="AGZ39057"/>
        <property type="gene ID" value="AFR_03840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.776"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044651_0_0_11"/>
    <dbReference type="OrthoDB" id="1864829at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="25"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="12847" checksum="6AC5E5A5C76DD316" modified="2014-01-22" version="1">MTVARLEFAGATGTTRLEVAVCGTVIQLTGDLDMATAPDLDRLIAGLLGDTPTVLVLDLHRLDFLGAAGITCLLGIRDRLAARHGLLVLRELPAQVRRVLEITQLLDEFDVGGSARSVHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR63</accession>
    <name>U5VR63_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358994.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05870"/>
    <dbReference type="EnsemblBacteria" id="AGZ39463">
        <property type="protein sequence ID" value="AGZ39463"/>
        <property type="gene ID" value="AFR_05870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1192"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095331_0_0_11"/>
    <dbReference type="OrthoDB" id="1900922at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="239" mass="25494" checksum="B6F0E3CDF0FA6178" modified="2014-01-22" version="1">MSNEVTVPLLPCGSIDDIENFYGVLGFSTTYKQRKPNAAVGLRREDLHLQFFEIAGFEPEQSYGSCLVLTEDIAGLHRAFAAGMRAAYGKVLVAGVPRMTRPRARKNADGVGGFSVVDPGGNWIRVFQKPDTMSATAPAAAGRLAKALANAVVLADSKGDVGQAARILDSALARPQDDDDPVTHVEVVVYRAELAIALDDRETAVEMLARVERIALNADETGRAAPTFEIAADLAAALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRD8</accession>
    <name>U5VRD8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LPPG:FO 2-phospho-L-lactate transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358950.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05650"/>
    <dbReference type="EnsemblBacteria" id="AGZ39419">
        <property type="protein sequence ID" value="AGZ39419"/>
        <property type="gene ID" value="AFR_05650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1146"/>
    <dbReference type="eggNOG" id="COG0391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055795_0_0_11"/>
    <dbReference type="OrthoDB" id="1079756at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07186">
        <property type="entry name" value="CofD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01257">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010115">
        <property type="entry name" value="FbiA/CofD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007">
        <property type="entry name" value="PTHR43007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="SSF142338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01819">
        <property type="entry name" value="F420_cofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="319" mass="33960" checksum="F63AC0AA118E35E7" modified="2014-01-22" version="1">MRIVVLTGGIGGARFLVGVRAHARRIGAEVTAVVNVGDDVRMHGLQICPDLDSVMYTLGGAADPDRGWGRVGETWVVKEELAKYGAEPSWFGLGDKDTATHLVRTTMLNAGYPLSAVTTALCDRWQPGIKMLPASDDRLETHVVVDVEGERKAIHFQEWWVRYRGDVPTHRFVFVGAETAKPAAGVLEAIGNADVVLVAPSNPVVSIAPILAVQQLKEAVLDGPAPVVGVSPIIGGSPVRGMADRCLATLGVEVSANGVAGLYGARRDNGLLDGWLVDTTDESANIPGVTTRAVPLWMTDEDKTATMVAEALRLAEELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRL2</accession>
    <name>U5VRL2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357240.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38296">
        <property type="protein sequence ID" value="AGZ38296"/>
        <property type="gene ID" value="AFR_00035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7"/>
    <dbReference type="eggNOG" id="ENOG5031CHP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1407879_0_0_11"/>
    <dbReference type="OrthoDB" id="1411491at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20313" checksum="071E2D15F71FC935" modified="2014-01-22" version="1">MALDKGLDNVAVQWPRTNRFYDPVAPAEFVDFGAVADAPDIAAPTGALALHIAKTGTVRATAYTELVDLLLGLEGVLYATDAAAEDEDPVIDPDGCAWIAGGLERFVEGHQEYGDLVTFDSVANVMRAAVAGGRLAEQQQRWLDHRLAALRDDAGNAPHWSFARTELAVLSGFYRRCAERGFAVFADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS41</accession>
    <name>U5VS41_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359334.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39803">
        <property type="protein sequence ID" value="AGZ39803"/>
        <property type="gene ID" value="AFR_07570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1545"/>
    <dbReference type="HOGENOM" id="CLU_198999_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="7318" checksum="FDE256E9FC238F4B" modified="2014-01-22" version="1">MAASPVLAADALFSLLMPLTQVLIGVLVLAAVVVSVHRLIQRGPTRMGGAMLLIGGALVCVAVLSYLVQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT13</accession>
    <name>U5VT13_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359634.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09020"/>
    <dbReference type="EnsemblBacteria" id="AGZ40093">
        <property type="protein sequence ID" value="AGZ40093"/>
        <property type="gene ID" value="AFR_09020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1835"/>
    <dbReference type="HOGENOM" id="CLU_2713242_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665177" evidence="2">
        <location>
            <begin position="29"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="72" mass="6994" checksum="BFCD836FB2A837C3" modified="2014-01-22" version="1">MTAARSAGNTAGAKVRSGSSAKAGNAKASSMTKTSATNAGRVKKASTPAAARQATAAGNGRVTSARKATTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT26</accession>
    <name>U5VT26_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase sigma-E factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02955</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357823.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02955"/>
    <dbReference type="EnsemblBacteria" id="AGZ38880">
        <property type="protein sequence ID" value="AGZ38880"/>
        <property type="gene ID" value="AFR_02955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.594"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="107"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="176" mass="19732" checksum="34346976B5715AFF" modified="2014-01-22" version="1">MDDARDSAYADFVRERSHALLRSAYLLTGDQHLAEDLVQEALARTHRAWSRLERPSNAEAYARKVMYHAQVSVWRRPKVAEALPGDGLERHGGSVDDPAEATVRRVTLQRALLALSPKQRAVIVLRYFEDHTEVEAAQMLGVSVSTVKTQCARALDRLRSLVPDLRVLADVHEAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT39</accession>
    <name>U5VT39_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator mprA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357759.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02635"/>
    <dbReference type="EnsemblBacteria" id="AGZ38816">
        <property type="protein sequence ID" value="AGZ38816"/>
        <property type="gene ID" value="AFR_02635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.530"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_9_11"/>
    <dbReference type="OrthoDB" id="1729688at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="158" mass="16804" checksum="411FE7B0A5250DD0" modified="2014-01-22" version="1">MPTVLIADDDADHRELITLALAKLGHRSVEAADGETALRLADAGGIDAVLLDVRMPGLSGIELCRRLRSEPATAHLPIMMISADVNGHRILAGMDAGADDYLTKPYHRSELFTRLETLLGRKRTPVNKPATAASAALLAARGGMARPVPAAQPLRRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT89</accession>
    <name>U5VT89_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02935"/>
    <dbReference type="EnsemblBacteria" id="AGZ38876">
        <property type="protein sequence ID" value="AGZ38876"/>
        <property type="gene ID" value="AFR_02935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.590"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1131704_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="25400" checksum="A4A9B18A57E75EDB" modified="2014-01-22" version="1">MTPDLQPAEDLLDSRDALGAAVRVGAVLATDPDHVPALLLMARAQIALSHPGTAVELAERACELAPADPATLNVLSLALTGAGRHDEAVTAARQAVRADPREAAHHDRLARALLGAGRFTEAEQAAETAVTLEPGVPGILLTLAAAHAGLDHRDRARRVLFAVLEIDPHHVEARAQLVRLAVPASRNVRRPAVAPGRLVVPALALGLVGLVLLLQGFTAPAVACLGLAVVFLLAGGFRGNHSEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTI5</accession>
    <name>U5VTI5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="5 6">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadE</name>
        <name evidence="11" type="ORF">AFR_09040</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate + H(+) + L-glutamate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="5 6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359642.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09040"/>
    <dbReference type="EnsemblBacteria" id="AGZ40097">
        <property type="protein sequence ID" value="AGZ40097"/>
        <property type="gene ID" value="AFR_09040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1839"/>
    <dbReference type="eggNOG" id="COG0171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022313_2_0_11"/>
    <dbReference type="OrthoDB" id="1152435at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000257">
        <property type="term" value="F:nitrilase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000132">
        <property type="entry name" value="Nitrilase/CN_hydratase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00920">
        <property type="entry name" value="NITRIL_CHT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="479"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for glutaminase activity" evidence="5">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" description="For glutaminase activity" evidence="5">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutaminase activity" evidence="5">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="334"/>
            <end position="341"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ40097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="587" mass="61850" checksum="47FE037B0D214F81" modified="2014-01-22" version="1">MPSLRVALAQVNSTVGDIPGNAAAVRHWSRTAAEVGAQLVAFPEMMLTGYPIEDLVFRDSFVASSQAALRTLAADLAGDGLGDVAVIVGYVDADGPAPTSSAAVPGSGRRDASALLFGGEIVATYFKHHLPNYGVFDEDRYFEPGDSLTVVRLGGVDVALTVCEDLWQAGGPFAAAGRAGVGLVVNINASPYELNKDDLRLPLVQRRAAEAGATIAYVNMVGGQDELVFDGDSMIVTAGGELLTRAGQFTSELLIHDLDVPAADPTPNVTDAQGDDGMGIRRVTVEAPPRPLTDGPAVGGIADRVTDEAEIWSALTLGLHDYVEKNGFRSVVLGVSGGIDSAVVAAIAVDALGPQRVVGVSMPSGYSSGHSKDDAADLAKRTGLDYRSEPIQPMVDAFLANMSLSGLAVENLQARVRGVILMALSNQEGHLVLTTGNKSELAVGYSTLYGDSVGGYNPLKDVPKTMVWRLAKWRNADAANRGGEPPIPENSIVKPPSAELRPGQVDSDSLPDYDLLDAILIGYIDGDQGRDDLIAAGHDAALVDRVLRMVDLAEYKRRQSAPGTKISSKAFGRDRRLPITNRFREGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTI9</accession>
    <name>U5VTI9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359651.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09065"/>
    <dbReference type="EnsemblBacteria" id="AGZ40102">
        <property type="protein sequence ID" value="AGZ40102"/>
        <property type="gene ID" value="AFR_09065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1844"/>
    <dbReference type="eggNOG" id="COG0518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054974_3_2_11"/>
    <dbReference type="OrthoDB" id="1242215at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="PTHR42695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="77"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="27912" checksum="F4E179065FE21FF6" modified="2014-01-22" version="1">MGTALVIENDPSDDLRRLGDWFAEAGLELTVLRPHAGDPLPETLDDFLALVVLGGDQSACASATGEPAAPWFPALEGLLRKAVRHHVPTLGVCLGGQLLATAHGGLVERSTSGPEIGPGLVARRDASDNDPLFKRVPLLPDVVQWHSDEITELPINAVLLAASTRYPHQAFRLGDRAWGLQFHIECDADMIAEWTRTDSATLDELGYDAEAVVAATAEVLADVEEVWQPFAARFAALALGQLPDADIPNPGTLRTLPLLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTT5</accession>
    <name>U5VTT5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molecular chaperone GroES</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09665"/>
    <dbReference type="EnsemblBacteria" id="AGZ40222">
        <property type="protein sequence ID" value="AGZ40222"/>
        <property type="gene ID" value="AFR_09665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1970"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="12"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="31394" checksum="0CFF370F01B4C916" modified="2014-01-22" version="1">MRMFAAQYHRFGPPEVLVTGSYEEPHAGPGQVRIRVAAAGIAPVDLAIRAGLSRGQISLPHVPGVDAAGIIDEIGPGVSGYAVGDEVFGTVDLKRLGGASAEFAVLGFWASKPGKLPWVQAGAAGTSIETATRALDLLDVGAGTTLLIDGAAGGVGSVAVQLAAARGARVVGTGRPGSQDFIAGLGATPVVAGPDLVGRAVELGVSAVLDVAGAGRLAALTTITDAVLTIADFSAAEHGVRMSVGALGGEPDGQHGLQIAAALVEQGQGQGRFRVPVQEVFALEDMAAAHAAAGSGHRRGKIAVWTGLVDSQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTW4</accession>
    <name>U5VTW4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">acVLRF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360324.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10770"/>
    <dbReference type="EnsemblBacteria" id="AGZ40443">
        <property type="protein sequence ID" value="AGZ40443"/>
        <property type="gene ID" value="AFR_10770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2195"/>
    <dbReference type="eggNOG" id="COG1503">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082731_0_0_11"/>
    <dbReference type="OrthoDB" id="1868138at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040783">
        <property type="entry name" value="VLRF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18859">
        <property type="entry name" value="acVLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="acVLRF1" evidence="1">
        <location>
            <begin position="74"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="23144" checksum="06840FD3FD762309" modified="2014-01-22" version="1">MSAKPAAGGGRWVDVAPERLTRWLENFATRHGPWTAAGLIVTAEDGATAELSPPPGAPPVEDLAALQREAAVARRIGLLLARKGAVAVGVAEGTKLIVSKVDTHYVQGRTAAGGWSQQRFARRRDNQAKAAAADGAGIAARLLLPEVRTLTALVTGGDRTAVETILATPNLRPLAPLISDRFLDVPEPRKTVLEDATAAARTIQILIQDPTPDQSAPSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU23</accession>
    <name>U5VU23_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Protein translocase subunit SecY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">secY</name>
        <name evidence="13" type="ORF">AFR_04605</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 12">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358205.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04605"/>
    <dbReference type="EnsemblBacteria" id="AGZ39210">
        <property type="protein sequence ID" value="AGZ39210"/>
        <property type="gene ID" value="AFR_04605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.930"/>
    <dbReference type="eggNOG" id="COG0201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030313_0_0_11"/>
    <dbReference type="OrthoDB" id="1567535at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 11" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ39210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="49478" checksum="2347C177A7C0B273" modified="2014-01-22" version="1">MLSAFSSAFRTPDLRKKLLFTVAIIAVYRLGATLPSPGVSYTNVQNCLDVVNSTNAGGGVLNLLNLFSGGALLQLSVFALGIMPYITASIILQLLTVVIPRLEQLRKEGQSGQAKITQYTRYLTLGLAVLQSSAFVALARSGQLFANACPQDQSNFQIVPENTGVPMWLTLVMLVITMTAGTGVVMWLGELITDRGVGNGMSVLIFTSIAARLPGEGWTIKQTSGTGKFLLVIALVLIVICAVVFIEQAQRRIPVQYAKRMIGRRMYGGTSTYIPLKVNQAGVVPVIFASSLLYLPQLYLQFFDQNNLKGYQQWIQNNLANPSHWIYITTYFLLIIFFTYFYVSITFNPTEVADNMKKYGGFVPGIRPGKPTADYLDFILSRITLPGALYLGLISVLPNFFFIWLNNAQYQNFPFGGTAVLIMVGVGLETVKQIESQLMQRNYEGFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUV8</accession>
    <name>U5VUV8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39520">
        <property type="protein sequence ID" value="AGZ39520"/>
        <property type="gene ID" value="AFR_06155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1250"/>
    <dbReference type="eggNOG" id="ENOG5033D1C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307926_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="23019" checksum="3A7B9F92CB1A612B" modified="2014-01-22" version="1">MVEQFGDKVAAELWTRVPEALSTAIDREVNAHPTATTTTERVLANPRWPLPYEELVLFLGTLPGAEIISVPKSVYQLVVINGHVVVPWCYGQSAGMSMKDAEVGRSFGRLARELLRRFGPPWRRSQAEAPLPLDAVDEREVAKICAAIATISPRPEVVIAGFAGAPNLGLLRACLGEIKSTDNGSLNWSHLDDLPLPPPVIPRPRRMQFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUX0</accession>
    <name>U5VUX0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12510"/>
    <dbReference type="EnsemblBacteria" id="AGZ40788">
        <property type="protein sequence ID" value="AGZ40788"/>
        <property type="gene ID" value="AFR_12510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2541"/>
    <dbReference type="eggNOG" id="ENOG5032VEG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1364167_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="22836" checksum="40CBC16336A68792" modified="2014-01-22" version="1">MIDANRGLRRPGLALTLVLVTLGPVLLALAGLLHPTGLSAATAHRWMWLHIVLLPIFPLLALGFVVLLRERPRAGVPRVARMIAWICAAVYAVGYTGLDAVAGIGAGTVAGQPGDPTELRRLVVALYEAGDRLGAVGVYAFLAATLAASVALGSRHGLRVLPGTVVLLVAGWSFIDSHIFSPRGVQTMLAMAVGFALWTWAAARRNGPAATKPADATDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV85</accession>
    <name>U5VV85_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360843.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12490"/>
    <dbReference type="EnsemblBacteria" id="AGZ40784">
        <property type="protein sequence ID" value="AGZ40784"/>
        <property type="gene ID" value="AFR_12490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2537"/>
    <dbReference type="HOGENOM" id="CLU_074078_3_0_11"/>
    <dbReference type="OrthoDB" id="1811015at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007995">
        <property type="entry name" value="DUF742"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36221">
        <property type="entry name" value="PTHR36221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05331">
        <property type="entry name" value="DUF742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12692" checksum="674D28DA80427523" modified="2014-01-22" version="1">MTWEETWYDEDSGPLVRLYARAGNGAPTGAPDDFELNALISRGAHPASAELSPVESAIVRLVVVPLSVSEIAAEVDLPLGEVRTLLRGLHDGGLIEVRRPADSMPHVLEQLLTGLRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVA2</accession>
    <name>U5VVA2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360997.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13260"/>
    <dbReference type="EnsemblBacteria" id="AGZ40938">
        <property type="protein sequence ID" value="AGZ40938"/>
        <property type="gene ID" value="AFR_13260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2692"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_28_0_11"/>
    <dbReference type="OrthoDB" id="1204683at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="203" mass="21248" checksum="9EE0F97E311D0F0E" modified="2014-01-22" version="1">MSAATPTTGRPRAFDEDAVLERAAEVFWRRGYEGASLTALTSAMGINRPSLYATFGSKEELFKRAFSRYHERQVATARAALDQPSAYASIEAFLRSSADGLTAEDHPPGCFSIQGGLACAPENARISESLAAGRAATEAALRERLSRAAAEGDLPEGVDPGALARFVMVISEGHAVHAAAGAARADLHASVDIAMRALGTAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVE1</accession>
    <name>U5VVE1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dsba oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359117.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06485"/>
    <dbReference type="EnsemblBacteria" id="AGZ39586">
        <property type="protein sequence ID" value="AGZ39586"/>
        <property type="gene ID" value="AFR_06485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1315"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_47_3_11"/>
    <dbReference type="OrthoDB" id="1678187at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like_fold" evidence="2">
        <location>
            <begin position="81"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="25321" checksum="3E95927A8C512FA4" modified="2014-01-22" version="1">MSAKNNRDRAKAKRVVQEQLAKERRRRITLWTSLGVVGVLIIAGLIGWSVAAGQESDKTANLTTPSVAVDDGTAFAVGTGPVKIDIYEDFMCPICGQFESSVATTVDQLVADKKVTVQYHPIAILDRASSTEYSTRSAGASAAAAQGGKFAEYHKVLFANQPEEGSAGLDNAKLIELGKSVGLSDAAFSDAVDKGTYRAWATKATDTAASRGVSSTPTVLVGGKALEDRTPQGLTAAVEAAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVL3</accession>
    <name>U5VVL3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360906.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12805"/>
    <dbReference type="EnsemblBacteria" id="AGZ40847">
        <property type="protein sequence ID" value="AGZ40847"/>
        <property type="gene ID" value="AFR_12805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2601"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_6_11"/>
    <dbReference type="OrthoDB" id="1582456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="222" mass="23412" checksum="EF6D9C4132543E3D" modified="2014-01-22" version="1">MELINATAFVTGANRGLGREIATQLLGRGAKVYAGVRDPAALDLPGAVPVQLDVTDAASVQRAAEVANDATLVVNNAGIYTRERFLDGDTDLIHREMEVNYFGPLAVIRSFTPVIEANGGGAFLNILSVVSWFHPVDFGPYAAAKAASWALSNATREELAPRGIAVSAVHVGFLDTEMVAHLEGIPKLSPGDVARTALDGLAKGENEILVDDFTRSIKASLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVS9</accession>
    <name>U5VVS9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_13155</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13155"/>
    <dbReference type="EnsemblBacteria" id="AGZ40917">
        <property type="protein sequence ID" value="AGZ40917"/>
        <property type="gene ID" value="AFR_13155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2671"/>
    <dbReference type="eggNOG" id="COG2770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="179"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="240"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="449" mass="48023" checksum="7ED7D637D02C6DDB" modified="2014-01-22" version="1">MSGSVAVAQSRAPAIASTYKTPAARANASGGTLAYGSEDYDFAQWYEFPYFGLGVSFAVVDSHGTVLLAQGQLAYYARESGLAFPATPPEPDEDLFGHAEVLLQPARSGGPGRLDGREVTLVTYNVPRAPESQDADATVYVLVSPLDTEAALAPVDRWLRGGLPVAVLFVAAVAWWAAGRALRPVEGMRAELARITAADMSSRVRRPRTGDEIARLADTMNATLDRLAEAAERQRRFVADGAHELRSPLAGLRNTVEVAAAHGGVADLKVLGAGIERLQRLTDDLLLLARLERTAPARGKPVDLAAIADELVGERRYRVPPDERFTVVASGPALVTGREEELTRLLNNLMSNASRYARERITVTVAKVEPGLVRVEVRDDGPGIPPADQERIFERFARVDEARDRGHGGAGLGLAIARDIAVRHGGTLYAAGSDDGACLIAELPWAPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWB6</accession>
    <name>U5VWB6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein-tyrosine phosphatase, low molecular weight</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08335"/>
    <dbReference type="EnsemblBacteria" id="AGZ39956">
        <property type="protein sequence ID" value="AGZ39956"/>
        <property type="gene ID" value="AFR_08335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1696"/>
    <dbReference type="eggNOG" id="COG0394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071415_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="SSF52788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LMWPc" evidence="1">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="13719" checksum="FD5DA48919625038" modified="2014-01-22" version="1">MLFVCVHNAGRSQMAAGWLRHLAGDRVEVRSAGSEPGDRINPAAVEAMAEVGIDITGQTPKKLEWETAESSDVIITMGCGDACPIFPGKRYEDWKLEDPAGKGVESVRPIRDEIRSRVEKLLAELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWC9</accession>
    <name>U5VWC9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361131.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13930"/>
    <dbReference type="EnsemblBacteria" id="AGZ41072">
        <property type="protein sequence ID" value="AGZ41072"/>
        <property type="gene ID" value="AFR_13930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2826"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115011_1_0_11"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665410" evidence="1">
        <location>
            <begin position="26"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="18005" checksum="83525AA5066D0476" modified="2014-01-22" version="1">MRALFVIGIAVLAGFVLSPLLRADAACAPVPATSSLDDWTDEQVANARLIITIGSDRQVPSEGAVIAVATALQESGLRNLRGGDRDSIGLFQQRPSQGWGTPRQLSDPAYQTRKFYDKLLAVDDWQQLKLTEAAQAVQVSAFPNAYAKHTRAATTLVGALSGSPACAPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWD4</accession>
    <name>U5VWD4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361372.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41313">
        <property type="protein sequence ID" value="AGZ41313"/>
        <property type="gene ID" value="AFR_15155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3077"/>
    <dbReference type="eggNOG" id="ENOG5031UD4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2182840_0_0_11"/>
    <dbReference type="OrthoDB" id="2068891at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="114" mass="12635" checksum="FCDDCAF0C2D4B981" modified="2014-01-22" version="1">MNLTRRPDRLCDCHCPAHSGSLRLAYTSASCKCLVTCSTQPVTLLVKEWACALFLHPDNDHLWFVMNVAHGQWDWESAGEIDERADMFDACGEIEGLLRRLTTVLAEAAGASGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWL8</accession>
    <name>U5VWL8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="8">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">gltX</name>
        <name evidence="12" type="ORF">AFR_08735</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08735"/>
    <dbReference type="EnsemblBacteria" id="AGZ40036">
        <property type="protein sequence ID" value="AGZ40036"/>
        <property type="gene ID" value="AFR_08735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1777"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020752">
        <property type="entry name" value="Glu-tRNA-synth_I_codon-bd_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-synt_1c" evidence="10">
        <location>
            <begin position="2"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_2" evidence="11">
        <location>
            <begin position="306"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="216"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ40036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="453" mass="50020" checksum="AD8F3043F75591F1" modified="2014-01-22" version="1">MGGARSALQNWIYAQQTGGVFVLRIEDTDAARNRPEWTEGIISALDWIGIERGTYEGPYYQSTYADEHTAAATRLYGEGRAYYCDCTRDDVVARSGNKHTGYDGFCRDRALGPGEGRALRFRTPDDGETVVTDLVRGKPTFENKLIEDFVIARSDGSAVFLLANVVDDMTMGITHVIRAEEHLPNTPKQQLLWEALGAEPPIWAHVPVIVNEKRQKLSKRRDKVALESYRDEGYLAAAMRNYLLLLGWAPSGDREIVPWSVIEEEFRLEGVSHSPAFFDVKKLRAFNGDYIRALDAEEFAAVCAPWLGGTDTIPAPPWDPAAFDSTVFAAVAPLAQTRIAVLSEIVEYVDFLFLDQPVTDEQSWNKAMKEGAAEILDAAGAAFGALDEWAAEPLKAALERVGEERGLKLGKTQAPVRVAATGRTIGLPLFESLEVLGRERTLARLATARTRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWR3</accession>
    <name>U5VWR3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-acetylmuramoyl-L-alanine amidase family 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41314">
        <property type="protein sequence ID" value="AGZ41314"/>
        <property type="gene ID" value="AFR_15160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3078"/>
    <dbReference type="eggNOG" id="COG5479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044057_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PTHR11022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="SSF55846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGRP" evidence="2">
        <location>
            <begin position="133"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="359" mass="36773" checksum="2D47C0E3C9B34A21" modified="2014-01-22" version="1">MSAVVAAAADVRPGAAVRRVPQTQLHSRPRARGGAKVGTAAFPLSHVGLSWTGGAAAVRLRTRDGWGDWQPARGCAPAGGGSALVNARDAIGYEVQADGGSVAAVELNTVDGPLRAVAAAPGVLPAAIVDDGPRYLSRAAWGAEESRRITTTPLSFFPVSVLTVHHEGGTYDPSREEAHVRALYAYQTTPAAQGGSGLDDLGYHLLIGAAGTVYEGRWSGTDLVPVFGPGAGPDGGPRMVNGGHLAGFNAGNVGVCLLGDYTTAAPAESVYRSLAAVLATLADACRLDPAATTRYVNPADVANPARTEPPRSATIRTVTSHRDWHAANPAAGSTDCPGAAFHPGMDRVRQDVAALITTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWU3</accession>
    <name>U5VWU3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative nucleotidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359805.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09455"/>
    <dbReference type="EnsemblBacteria" id="AGZ40180">
        <property type="protein sequence ID" value="AGZ40180"/>
        <property type="gene ID" value="AFR_09455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1928"/>
    <dbReference type="eggNOG" id="COG1208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_887494_0_0_11"/>
    <dbReference type="OrthoDB" id="1557977at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="7"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="28671" checksum="3521AE7FA2E2C6E1" modified="2014-01-22" version="1">MTPAVCAVVLAAGEGQRLRPLTELLPKALCPVGNVALLDRALARIAGLGLTGPTDVAVNAAYLADQVVAHVGDRAHLSVEPDGPLGTSGGIGRLLPWINGRGVLVGNADAYLADPHRDPGKDIEALLESWTGETVRMLTRPIPEGGQGGFSGHRFAGFSLLPWRYTQNLSDKPSDLVRTAWRPAEAAGELELVTYEGLYIDTGTPADYLKANLHAAAGLTDPSATLDGTASESVIGAGAQVHGQINRCVIWPGATVAANETLHSTIRAGTTLTVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXB2</accession>
    <name>U5VXB2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361668.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16575"/>
    <dbReference type="EnsemblBacteria" id="AGZ41593">
        <property type="protein sequence ID" value="AGZ41593"/>
        <property type="gene ID" value="AFR_16575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3361"/>
    <dbReference type="eggNOG" id="COG0859">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038371_0_1_11"/>
    <dbReference type="OrthoDB" id="1068323at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="310" mass="31849" checksum="EDFFE0A4EA1DF9F8" modified="2014-01-22" version="1">MILVLRALGVGDLATAVPALRAVQRAARPGERVALAAPAWLSPLVELVGGLDLVPTAGLTGVTWDLPAPRLAVNLHGCGPESHRLLASAHPGVLWAFANAGAGHPGGPAWSEEDHEVHRWCRLTGHYGAPADPADLALTVPSPVAATGLTIVHPGAKSPTRRWPARRYAEVARSLVARGHDVVVTGSDDEAAAATWIAGEAGLAPGAVLAGRTGVGELAALVAHARLVISGDTGIAHLASAYATPSVVLFGPMSPGRWGPPPRAYHRAIWHGTRAEPGDAPGPDPHPALLRITAREVLEAADAAVSLRCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXC3</accession>
    <name>U5VXC3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361501.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15800"/>
    <dbReference type="EnsemblBacteria" id="AGZ41442">
        <property type="protein sequence ID" value="AGZ41442"/>
        <property type="gene ID" value="AFR_15800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3207"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083329_0_0_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="49"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="30357" checksum="F47BC9AE7F7CEFA2" modified="2014-01-22" version="1">MTQTFTLSVPGADLVYDVRGPLPPADGRPVLLMIGQPMTAEGFGALAGHFEDRTVVTYDPRGLGRSKRSDGRSDNTPQRQAADLHLLIEELGAGPVEVFGSSGGAVTGLELVATHPGDVVTLVAHEPPINAVLPDAEAADRARARFHEAYQAKGMGAGMAAFMVMTSWQGEFTDAYFEQPAADPAMFGMPDTDDGSRNDPLLSKDSWAVIDYRPDAGALEAAPTRIVIAVGEESAGTYTARTALGTAALLGQEVTVFPSHHGGFLGGEFGYAGKPEEFAVKLREVLSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXF0</accession>
    <name>U5VXF0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AP_endonuc_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361759.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16825"/>
    <dbReference type="EnsemblBacteria" id="AGZ41643">
        <property type="protein sequence ID" value="AGZ41643"/>
        <property type="gene ID" value="AFR_16825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3413"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_936313_0_0_11"/>
    <dbReference type="OrthoDB" id="1194699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="30"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="285" mass="30934" checksum="546C7645487F8F7E" modified="2014-01-22" version="1">MNRIAANPIPYWSRAGKTREVFDEAFRDFQEIGFTAVKADVPDGMTPSEYAGWIAGYGLAPALSLFSSPFDETVELAEEIERAKVFAAGQVALGLDRTMISSMAVPARMAQPAVGADFDAGRLDRAIDNCGTVCRVLQAEGLRPLHHSHVGGVFETEDEIVRLLDDLGAGVIGFGPDTGHLRWAGIDPAPFITRYADRLGGIHLKDCFPDYLSPASRHGMSYHEVQATKRLWAEPGRGVIDLDAVLAAVPAGYDGDFMIEVDEPSMDSKKESHRISLEWAKGALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXH8</accession>
    <name>U5VXH8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphate import ATP-binding protein pstB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_10410</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360182.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10410"/>
    <dbReference type="EnsemblBacteria" id="AGZ40371">
        <property type="protein sequence ID" value="AGZ40371"/>
        <property type="gene ID" value="AFR_10410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2121"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030660">
        <property type="entry name" value="ABC_branched_ATPase_LivF/BraG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039137">
        <property type="entry name" value="ABC_branched_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="25485" checksum="F31CD4D29BFEF55E" modified="2014-01-22" version="1">MLLELKDVTLAYGRIQALHGISLTVAEGEIVALIGANGAGKSTTMRAISGLRPVAQGSITFNGEDITKLRADLRVVRGVSQSPEGRGIFPGMSVLENLEMGAYTRRNRAEINQDLERVFTLFPRLKEREKQVGGTMSGGEQQMLAVGRALMSRPKLLLLDEPSMGLAPMLIQQIFDIVVEINQQGTTVLLVEQNAQQALSRAHRAYVLETGEIVKSGTGAELLTDPSVKDAYLGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXM9</accession>
    <name>U5VXM9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361940.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17275"/>
    <dbReference type="EnsemblBacteria" id="AGZ41733">
        <property type="protein sequence ID" value="AGZ41733"/>
        <property type="gene ID" value="AFR_17275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3503"/>
    <dbReference type="eggNOG" id="ENOG503329P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053642_0_0_11"/>
    <dbReference type="OrthoDB" id="1850042at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665832" evidence="2">
        <location>
            <begin position="28"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="29431" checksum="A32AE4D8BF962053" modified="2014-01-22" version="1">MTVLHRLACLVLTAGGLLLAWASPAAAHAGGLVATDARSHVVALSPAVPGLEVTAIEDGARLRLRNHTTVPVGVPTGGGAATPAVVAAGQKLTWIDTRSTPEGRSLGAGATQAWSIVLDVGGTPVTVTGELVGARPPSPVPWWLAAVLLAVAVPLVARRSRRPGDLLAATGLVAMAASITHVAGSTLAVESAPMAGTFLSAAGINLLAWPLILGGAVTVFRGRPAGVLAVCAGAALTAVFVLPDVTSFHRAVLPFAGPAVVERILVVLALGTGIGVAVAGASVLRTLALRAGAEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXZ4</accession>
    <name>U5VXZ4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lar_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361757.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16820"/>
    <dbReference type="EnsemblBacteria" id="AGZ41642">
        <property type="protein sequence ID" value="AGZ41642"/>
        <property type="gene ID" value="AFR_16820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3412"/>
    <dbReference type="eggNOG" id="COG3875">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_620695_0_0_11"/>
    <dbReference type="OrthoDB" id="313921at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050043">
        <property type="term" value="F:lactate racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043166">
        <property type="entry name" value="LarA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018657">
        <property type="entry name" value="LarA-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09861">
        <property type="entry name" value="Lar_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lar_N" evidence="1">
        <location>
            <begin position="31"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="416" mass="44659" checksum="983A316800396ADE" modified="2014-01-22" version="1">MSARIGGADRILTEDEVRQFVTDQLGGLDLDGRSVCVLVPDGTRVCPLPLLVGAVHGALHGRVSRLTVLIALGTHAPMNDEALAVHLGYAGRELGEIYPGTTVLNHEWWLPETFAHLGTVPSARLEELSEGRMSGDVEVLLNRAVVDHDVALVVGPVLPHEVVGMSGGNKYFFPGVAGQRIIDVSHWLGALITSAEIIGTTDLTPVRALINDGAALIPADTYALCVVTAEPAGLHSVSFGDCISSWESAAEISAATHVTYLDAPVRRVLSLIPAMYDEIWTGAKGFYKVEPVVADGGEVILYAPHITEIATSHPQIHEIGYHCRDYFVKQWDTFSGIHWGVLAHSTHLRGAGTYDAATGEERLRVNVTLATGIPEEVCRSVNLGYRDPGTIDVAAFEADPDTLVVPRAGEVLFRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY55</accession>
    <name>U5VY55_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase, PA-phosphatase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17730"/>
    <dbReference type="EnsemblBacteria" id="AGZ41824">
        <property type="protein sequence ID" value="AGZ41824"/>
        <property type="gene ID" value="AFR_17730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3595"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020920_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665865" evidence="1">
        <location>
            <begin position="36"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="297"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="429" mass="46101" checksum="BC4AC667E2DB5FDF" modified="2014-01-22" version="1">MALRVRTPGKSWKSRSLAVTATFALFTPVALPATAAQARPATAPNAVVTWNTHAQTAIFEVARQPPYVTGRSFAMVQGAVYDAVNTIAGTPYEPYLVAPRTRHGDSADAAVATAAYRVLLSLFPGQAESLRTQYDASLAVIRDGRSKSGGITVGEETAAAMIAARTGDGAFGDATWNAGTAPGQWRPTPPAFAQDGAWVGDLKPFVIRRASDYRTSGPPALTSKAYARELNEIKALGSATSTVRTPDQTEAAIWWHDRRLGEWEIKRQLATTHRLSPLQTARMFAMADIANADSLTACFAEKKLWNFWRPVTAVPLADTDGNPATTADPAWTPLLATPPFPDYTSGHTCSTAAIMAALRAFFGRDDVPFSAYSADSGTTRSFASFSQAMAEVVEARIWGGVHYRSADVQGQKLGEQVARYVLGHEFGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY71</accession>
    <name>U5VY71_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phenazine biosynthesis protein PhzE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360577.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11460"/>
    <dbReference type="EnsemblBacteria" id="AGZ40581">
        <property type="protein sequence ID" value="AGZ40581"/>
        <property type="gene ID" value="AFR_11460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2336"/>
    <dbReference type="eggNOG" id="COG0147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0512">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028026_0_0_11"/>
    <dbReference type="OrthoDB" id="1786019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate_bind" evidence="2">
        <location>
            <begin position="102"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="417"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="601" mass="64010" checksum="AFCF93A9C529DD50" modified="2014-01-22" version="1">MKPFALLHRDGADHVDVLEGEVVTVGTLADIPLTPGREGPQTLALIPFRQITERGFDCVDDGAPLECLQISAHTTRPLAEIIDSLPTTPVALRDGAFDITDDAYGEIVSRVLRDEIGHGEGANFVIHRVFTASVDGDPLDTARAAFRNLLTAEQGAYWTFLVHTGTRTLVGATPERHVSVADGLTMMNPISGTFRHDGTRELVDFLADRKEIEELYMVLDEELKMMAAVAEHGGQVVGPYLKRMAHLTHTEYLLAGKGSLDVREVLRETMFAPTVTGSPLENACRVIARHERRGRGYYAGVLALLGHDSAGRQTLDAPILIRTAELSPAGQLRVPVGATLVRHSTTAGEIAETHTKAAGVLAALGASALPTVRPEAESPEVLAALAARNDGLARFWLDQRLPGALTVPELDGRTAVIVDNEDTFTAMLAHQLRALGLTVTVVPWTVPVVPDADLVIVGPGPGDPASGDPKMVRLRGLVDELLAARQPLLAVCLGHQILASALGLRLHRRVTPYQGVARDIDLFGSVRRVGFYSSFTALAGDADGVELAVDPADGTVHALRGTGFAGVQFHPESVLSADGVTVLAELLPPLLSRTVSTAVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYC9</accession>
    <name>U5VYC9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase GCN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11785"/>
    <dbReference type="EnsemblBacteria" id="AGZ40646">
        <property type="protein sequence ID" value="AGZ40646"/>
        <property type="gene ID" value="AFR_11785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2400"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="20795" checksum="9A07D0A5E445FC34" modified="2014-01-22" version="1">MITGGLFVFVLGDLVTLRAMEPSDADALWRWNHDPEVMRWMDDGYAQSLAQVRQFLQERERNEYTDVLFGIETTADARLIGLIRLHGAEPETGNAELDIYLGEKDSWGRGYATDAVRTACRYGFDKMRLHKVTLTVVAGNDAAQHVYKKVGFVEEGRLREVFRRDGRWHDKLTMGLLEGELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYD0</accession>
    <name>U5VYD0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17545"/>
    <dbReference type="EnsemblBacteria" id="AGZ41787">
        <property type="protein sequence ID" value="AGZ41787"/>
        <property type="gene ID" value="AFR_17545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3556"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_528575_0_0_11"/>
    <dbReference type="OrthoDB" id="1247435at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01823">
        <property type="entry name" value="SEST_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037460">
        <property type="entry name" value="SEST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37981">
        <property type="entry name" value="PTHR37981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665870" evidence="1">
        <location>
            <begin position="20"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH_hydro" evidence="2">
        <location>
            <begin position="246"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="515" mass="53770" checksum="A5D36EA076297078" modified="2014-01-22" version="1">MAALVVVALTLAGGPPAHADNTAAMTFPADGRLSIELIVAAGSYEPDFLSFVANDYDDTPLCSGSCGSGVWESPSEPAGTPFDIALGELDGSFQLSDDRYALQRDGTTVWTFTRRADKRNFAYTVRLTFLPGTGPGPGSGEPAPANRPPVFTEAPQQVDGVFPQPGSFRVAAQDPDGGPVTVTWSQLPLQPVKVDCVRSADGSVATCSTTARSGLDHAGEITFTATDAPGARATTKVLVRPANYVALGDSFSSGEGAPPFDAGTDTKADKCHRSKFAWARYLDIEAPLALSGHFACSGALATNVVRDSFKTERPQTAQLRSVANVPELVTVSVGGNDVGFGDIILTCYLTGLATRGREVGAGPCINRVRRAKQDIDRLLPARLPDVYRSIGAQLTAGGRAGRVLVVGYPNIMATGTAFKAHCLWLSGGAREALVGLGTYLDTRIAQIVATTRTSGVPVSYVSTLNVLAGHELCTGDAYVEAIVPNGGKTQLSGHPTAAGQQRMRGRVDAAIAAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYH2</accession>
    <name>U5VYH2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42033">
        <property type="protein sequence ID" value="AGZ42033"/>
        <property type="gene ID" value="AFR_18785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3816"/>
    <dbReference type="HOGENOM" id="CLU_684481_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="402" mass="42062" checksum="8170DD2F76F691C0" modified="2014-01-22" version="1">MLAGSALIAVPGSASAAGSDMPECKGQMGVVALNDGSGCRRFAVPFPADSEMLDPSEGEQVPAGVAPAQELAEGELPDVGVARPVWLPFAGRYLVFYPVTSDVVCATGAGATVAYYNGVCHYFNIPYPNFWVQLGDGGRPGGIEQAPALTPRARYRAYDKPVWISHGVGSSGAVYYPLNYNPSGRCDPAADIAAVVVQGTCLRWTIPAGGTTVPYTEELAEKLQNAHQAHLLTDGEVPETETGPVFYTYPDGSPSFRTMMVLPAGVDPADPSCFPPGVERPGRIPVPCPSPPVVVVPPKQGARKPAVVAETSAVTLNRGQKTIRSFTLTSAGEPLSGATVAVNVFVPGARQSGRSYVTNSKGRIKVAVAAGKALGRGWIIVRYGGNSFVKPVSVEVPLRVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYP9</accession>
    <name>U5VYP9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">L-amino-acid oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362285.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18170"/>
    <dbReference type="EnsemblBacteria" id="AGZ41912">
        <property type="protein sequence ID" value="AGZ41912"/>
        <property type="gene ID" value="AFR_18170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3688"/>
    <dbReference type="eggNOG" id="COG1231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004498_8_3_11"/>
    <dbReference type="OrthoDB" id="1442136at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665500" evidence="1">
        <location>
            <begin position="38"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Amino_oxidase" evidence="2">
        <location>
            <begin position="68"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="527" mass="56540" checksum="EA1416184D1BB397" modified="2014-01-22" version="1">MRESVSRRRFLQSVGVSGGAGALFATMGALGLAPAAAAPAPPFDPPRKSDFSLTGRSAKRVVILGGGIAGLAAAYELGKAGYDCTILEARGVAGGRNLTVRGGTTETDLDGQTQRASFSPGIYLNAGPARIAQWMVTLDYCRELGVPIEPFVNANADALIYNEANGKPVRYRTAKADVYGYVSELLAKATDQGALDGQLTATDKERVLSFLQSFGAIGGRTAGWEYQGTNRRGYASYPGAGNDAGTPLDGPAALADVFASNVGRYFSFEFGYDQAMMMFQPVGGMDRIPYALSRAIGAGRIRLGAEITAVTDKPDGVEVTYRREGRTRTIEADFCVAALPPHLMARIPHNLGTAVQSALGSFPVTASGKIGLEYAGRWWENDLRIYGGITETDLDVSHIWYPSTGFHADRGLIVGYYNTGANARAYGSLTPEQRLLRAVEQGVKIHGEKYRSELTHSFSIAWHRTRYLEGAWTSPPWGSAGYNLLLQPAGHVHFAGDWLSHEVAWQHGAFVSARAAVTALHQRVLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ03</accession>
    <name>U5VZ03_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360965.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13100"/>
    <dbReference type="EnsemblBacteria" id="AGZ40906">
        <property type="protein sequence ID" value="AGZ40906"/>
        <property type="gene ID" value="AFR_13100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2660"/>
    <dbReference type="eggNOG" id="ENOG502ZBW7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093484_0_0_11"/>
    <dbReference type="OrthoDB" id="1918376at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="18897" checksum="B51B1C8CA6C962C1" modified="2014-01-22" version="1">MTTLVVPDISTTAATLRRLYFFRFAFAVVWAGVMLAIAGDLHPLVVTLFVLYPLVDVAATVVDAQSSAATRSPALLYLNVAVGTFAAGALAVAGSSGIPAVLRVWGAWAIVAGAIQLIVAVTRRAMGGQWPMIISGGLSVLAGGSFLVAASAADPMLTNAVAYAIPGAIFFLIAAVRLGRAPKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZA0</accession>
    <name>U5VZA0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361070.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13625"/>
    <dbReference type="EnsemblBacteria" id="AGZ41011">
        <property type="protein sequence ID" value="AGZ41011"/>
        <property type="gene ID" value="AFR_13625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2767"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_0_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="36"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="295" mass="32623" checksum="8F488C08B5CF443A" modified="2014-01-22" version="1">MADKQQIRADIREFLTTRRARITPEQAGLRVYGNSRRVAGLRREEVATLAGISAEYYTRLERGNVAGASDSVLDGITQALQLSDDERTHLYDLVRLASSSRPAARRRPAQVRVRPAVQRIMDSMVLTPAFVVDSRLDILAANELGRALYAPILADTVEPPNHARFLFLDRGAGDFWRDWNQAADDAVALLRGSAGRDPYDEHLSNLIGQLSTRSDDFRVRWASHDVRAHLTGAKRIHHPEVGDLELPFESMPLPSDPGQNLVVYSAEPGSASYASLQILASWAARPHEGQPATEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZD3</accession>
    <name>U5VZD3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PAS/PAC and GAF sensor-containing diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19250"/>
    <dbReference type="EnsemblBacteria" id="AGZ42122">
        <property type="protein sequence ID" value="AGZ42122"/>
        <property type="gene ID" value="AFR_19250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3910"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_789010_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="223"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="351" mass="38022" checksum="8C8CC85CFC00386B" modified="2014-01-22" version="1">MTASDPQLQALRALHAVTKRVHASLDLTQTLDAVANGVVEAAGFGLAALNLAEDNGDYTTVAVAGSDDLRRALLGMRGSAENWRELFRRSERWGSLYFVDHRFGAPESLYVWVPDIAPSDDPDMWHPLDCLFAPLMAPSGEWLGVLSVDLPEGGRRPDPQQQEILAFFAEHAAIAIQHARLHSQLERSQAEAEYAATHDNLTGLANRALLKSRIDALPALAVPQVGVLVIDLDGFKKVNDAAGHEAGDEVLRIVAERLQHHLRADDLLARTGGDEFVAVLVGDDLTTTISETAQRLRETIAEPICGSTGVHRIGASVGYAIGGAGEDFSRLVATADTEMYKEKRRSKAPAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZJ0</accession>
    <name>U5VZJ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361115.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41056">
        <property type="protein sequence ID" value="AGZ41056"/>
        <property type="gene ID" value="AFR_13850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2812"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_0_11"/>
    <dbReference type="OrthoDB" id="968232at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="232" mass="24253" checksum="263812D5D6C99AB6" modified="2014-01-22" version="1">MQTITLVTGGNKGIGYETARRLKAAGHTVLIGARDPERGKTAAAELGVGLLHLDITDQTSVDQAAAQVRAEHGRLDVLVNNAAVNTGVAPLEQTTAEDAAAVLDANLLGALRVTNAFVPLLRASQHARIVNVSSTVGSFAATLELDMFDWQIVPPLYAISKTALTMLTLKYSRALPGILVNAADPGYTATDLNNGQGTQTVTEGTDAIVHLATLDDDGPTGTFINRDGTVAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W061</accession>
    <name>U5W061_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362960.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21590"/>
    <dbReference type="EnsemblBacteria" id="AGZ42588">
        <property type="protein sequence ID" value="AGZ42588"/>
        <property type="gene ID" value="AFR_21590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4378"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049344_3_0_11"/>
    <dbReference type="OrthoDB" id="1687413at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="2">
        <location>
            <begin position="42"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="26204" checksum="B009ABE969C3C143" modified="2014-01-22" version="1">MEELPHASSFGAAAAAYAEHRPDYAQDAVRWVLDAAPGIRVLDLGAGTGKLTATLVAAGADVTAVEPDPAMLAELRRTLPQIRALPGNAEVIPLPDATFDAVVSGNAMHWFDMAVAGPEITRVLAPGGVLAGLWNVVDDRVDWVAGLAKVSGSAAIGPRDTPDAWRAETAGMASGFVAPEQAEFPHGQRRTADSLVATLGTRAGMLVMPDEERQAALGRIRAFLASRPETADGEFTLPMLTCVLRAHRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0N9</accession>
    <name>U5W0N9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363281.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22690"/>
    <dbReference type="EnsemblBacteria" id="AGZ42808">
        <property type="protein sequence ID" value="AGZ42808"/>
        <property type="gene ID" value="AFR_22690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4599"/>
    <dbReference type="HOGENOM" id="CLU_136168_0_0_11"/>
    <dbReference type="OrthoDB" id="2064186at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665555" evidence="1">
        <location>
            <begin position="22"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17943" checksum="E864FA15CF580A65" modified="2014-01-22" version="1">MSSSRRTVALVLAFLTAGALAAGCDSTQESTVSREEVVQVVADRAQEALQALPAGATLKQRLHQPDLTCNDQDRDGEQFVETNYQVEYPEGWPVDRSMADLSAYWERSGYKIVRDDRNDATTPELVVEKESDGYHIGYLISRRSSGKVDVYLRSSSPCFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W194</accession>
    <name>U5W194_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acetamidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361934.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17260"/>
    <dbReference type="EnsemblBacteria" id="AGZ41730">
        <property type="protein sequence ID" value="AGZ41730"/>
        <property type="gene ID" value="AFR_17260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3500"/>
    <dbReference type="eggNOG" id="COG2421">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032013_2_0_11"/>
    <dbReference type="OrthoDB" id="228571at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004304">
        <property type="entry name" value="FmdA_AmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31891">
        <property type="entry name" value="PTHR31891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03069">
        <property type="entry name" value="FmdA_AmdA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665964" evidence="2">
        <location>
            <begin position="45"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="46387" checksum="0B8D218CBBC30D56" modified="2014-01-22" version="1">MTEFAGKARDTLVAALGRRTFLRAAAAVSAGGLTAGATATPANAQTRTSPGGILQPGTGPIPGRHYLPSRPDEVRWGYVPSVTSEPVLRMRSGQTVTIDAVSHEGILEDQGRDPVSFFAGHGVPARRVLTDAVAIARGYDRTPRSFDVDGPHVVTGPIHVDGAAPGDVLKIEVLSAVPRVPYGVVSSRHGKGALPRLAGGAVPPGITEAEIMPPVATDGRATGDPLRYGNVSVFTPVRSGRGGALTGVLPRPGHRDVAFPLHPFMGIMGVAFADAGSLTDPLLNSIPPTLGGGNIDISLLGPGATFYLPVFAEGALFYVGDPHLAMGDGEVALTALEGSLRATYRLTVVKKGGRGAPSVAFTYPFAETPQAWVPIGLSDPDGARDGQGNDLDIAMRRAVVNALDFLEHDLGMNRAVAYAYLSAAADFSVSQVVDGTVGVHGVIPKTHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1B5</accession>
    <name>U5W1B5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP_transf_9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361984.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17385"/>
    <dbReference type="EnsemblBacteria" id="AGZ41755">
        <property type="protein sequence ID" value="AGZ41755"/>
        <property type="gene ID" value="AFR_17385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3524"/>
    <dbReference type="eggNOG" id="COG2343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103537_0_0_11"/>
    <dbReference type="OrthoDB" id="2063459at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007361">
        <property type="entry name" value="DUF427"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038694">
        <property type="entry name" value="DUF427_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04248">
        <property type="entry name" value="NTP_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_9" evidence="1">
        <location>
            <begin position="20"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16940" checksum="B4A7B2A0F31C5FC8" modified="2014-01-22" version="1">MESVWDYPRPPRLERSAARVTVTHAGVVVARSDRCLRVLETSHPPVYYVPREDITPDVLVAARGRSFCEFKGNASYWDLVVGEVRVPEAGWSYERPSPAYADLTGAVAFYPGRVDECRVGDEVVRAQEGDFYGGWITADITGPFKGAPGTMGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1B7</accession>
    <name>U5W1B7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363278.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22685"/>
    <dbReference type="ESTHER" id="9actn-u5w1b7">
        <property type="family name" value="Duf_1023"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42807">
        <property type="protein sequence ID" value="AGZ42807"/>
        <property type="gene ID" value="AFR_22685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4598"/>
    <dbReference type="eggNOG" id="COG4099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025057_2_1_11"/>
    <dbReference type="OrthoDB" id="2018813at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010427">
        <property type="entry name" value="DUF1023"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06259">
        <property type="entry name" value="Abhydrolase_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="556" mass="58987" checksum="237F3DB85C7174DA" modified="2014-01-22" version="1">MSALTLKVLWDTSPEAIAQRATAWTAMAEDIDNAAEEVIRGTREIEDVWPSGPAAEAAAKRAAELRAEVSNAYNPCRRIGLALREHADTVRALQESLRQVTTSASSQGFDVDIAAGTVSAPQRMYDETTSPHLVAQSVSAYVGQLQGILDRAAESDDRTTSLININLPDPKTGFGSLSLRPVSREDLLGQKGRPPKDVAAWWDTLTPEQQEQAIADHPELVGWLDGVPATDRDVANRIVLDRTVTDLQGQVTAANNREAYLKAMAEQGRLGEVYPHSGNPVGSMYAELDQLADQRKAAEGTLTGATTIGNRLADPNQPPALLLGFDPAGDGRAVVSIGDPDTADNVVTYVPGTTSDLPGIKTDLQRADTMAFDAEQYGDGTTASIVWLGYDAPDTIPNAVSGSYAEEGAPALRTFQEGLRATHDGGPSHNTIIGHSYGSTTVGYAARDGHLSADALIFVGSPGVGVDSASGLHFDGDSGDVYASTAKNDVIRGSGLDDNMIHGENPDNPGFGGQRFTSAEGDFWDPISTHSQYWDQGNPSRRNIALIVTDQPGQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1J2</accession>
    <name>U5W1J2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Poly(ethylene terephthalate) hydrolase</fullName>
            <ecNumber evidence="7">3.1.1.101</ecNumber>
            <ecNumber evidence="3">3.1.1.74</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_23110</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(ethylene terephthalate)(n) + H2O = (ethylene terephthalate)(n-1) + 4-[(2-hydroxyethoxy)carbonyl]benzoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49528"/>
            <dbReference type="Rhea" id="RHEA-COMP:12420"/>
            <dbReference type="Rhea" id="RHEA-COMP:12421"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:131701"/>
            <dbReference type="ChEBI" id="CHEBI:131704"/>
            <dbReference type="EC" id="3.1.1.101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:49529"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47348"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:50477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:47349"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.101" evidence="7"/>
    <dbReference type="EC" id="3.1.1.74" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23110"/>
    <dbReference type="ESTHER" id="9actn-u5w1j2">
        <property type="family name" value="Chlorophyllase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42892">
        <property type="protein sequence ID" value="AGZ42892"/>
        <property type="gene ID" value="AFR_23110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4683"/>
    <dbReference type="eggNOG" id="COG4188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066851_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041127">
        <property type="entry name" value="Chlorophyllase2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12740">
        <property type="entry name" value="Chlorophyllase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="30721" checksum="FF36A391CC740B65" modified="2014-01-22" version="1">MPSVLSMSPVILPPSSGRGEDLQVRVSAPATGDNLPIIVFSHGFGQSMDSYAPLAGFWAAHGFVVLQPTHLDSRTLKVTPEDPRYARIWRIRVDDLTNVLDHLDVLEAAVPGLAGRVDRDRTAVAGHSWGGQTASTLLGARVLDADGVPGEDMSDPRIKAGVLLATTGTGGADLSPFAAEHFPFMSPDFTAMTTPALVVAGDQDKSALSVRGPDWFTDPYFLSPGPKSLLTLFGAEHSLGGISGYAVTETTDESPERVALIQQLTTAYLRGVLDKEDTGLSAGGLGRIESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2X0</accession>
    <name>U5W2X0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1775 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_20340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362712.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20340"/>
    <dbReference type="EnsemblBacteria" id="AGZ42340">
        <property type="protein sequence ID" value="AGZ42340"/>
        <property type="gene ID" value="AFR_20340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4124"/>
    <dbReference type="eggNOG" id="COG4549">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087540_0_0_11"/>
    <dbReference type="OrthoDB" id="1828335at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08545">
        <property type="entry name" value="YcnI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012533">
        <property type="entry name" value="YcnI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038507">
        <property type="entry name" value="YcnI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07987">
        <property type="entry name" value="DUF1775"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666033" description="DUF1775 domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1775" evidence="4">
        <location>
            <begin position="28"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="24464" checksum="70E10E45B023A43A" modified="2014-01-22" version="1">MSLLKRSAVVAGLAAAFTLALAGPAAAHVTVNASTAVQGGYAKVTFRVPNESDDTNTTKVEVNLPADTPFASVSLKPVAGWTMVATKGKLAKPIEAHGTQISEAVSKVTWTATGDAAIKPGQFQEFDVSIGPLPQTPQVVFKALQTYSDGTIVRWIDEPTTDGSEPEKPAPVLKLTAAAATDAPAAATGPSVAAAPEAAADTESDSSTGTWLGVVGIVLGLAALVLGLLAYRRSSATPAGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W327</accession>
    <name>U5W327_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HxlR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20295"/>
    <dbReference type="EnsemblBacteria" id="AGZ42331">
        <property type="protein sequence ID" value="AGZ42331"/>
        <property type="gene ID" value="AFR_20295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4115"/>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="18647" checksum="BB8EE0BEF9D88DB8" modified="2014-01-22" version="1">MTTSEEHRSGCPINLSLEVLGDKWSLLIIRDMMFGNRRHFRALLTESEEGIASNILADRLQRLTAQGMITSSPDPRHKQRILLSLTEPAIQLVPVLAHLGAWGRRHLPVTRELAIRAELLERGGPELWDDFMDELRELHLGVPRPAGTESVLTRLTAAYQAELAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3G2</accession>
    <name>U5W3G2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Caffeoyl-CoA O-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_26375</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364335.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26375"/>
    <dbReference type="EnsemblBacteria" id="AGZ43537">
        <property type="protein sequence ID" value="AGZ43537"/>
        <property type="gene ID" value="AFR_26375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5346"/>
    <dbReference type="eggNOG" id="COG4122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067676_5_1_11"/>
    <dbReference type="OrthoDB" id="1948290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="22943" checksum="0E9807B6BF6A72A5" modified="2014-01-22" version="1">MTISDYLLAHCTPADDILLDLAAETAATFPDAAGMQISHDEGALLTMLVKLTGARKAVEVGVFTGYSAICIARGLGPDGHLLACDVSPEWTATARKYWQRAELDDRIDLRLAPAAETLRALPAEADIDFGFIDADKEGYPVYYEELLQRLRPGGLLVLDNVLQRGHILEENPSRPAVAAMQRMNDTVVADKRVESVMLPVRDGVTLIRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3K0</accession>
    <name>U5W3K0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_21340</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21340"/>
    <dbReference type="EnsemblBacteria" id="AGZ42540">
        <property type="protein sequence ID" value="AGZ42540"/>
        <property type="gene ID" value="AFR_21340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4329"/>
    <dbReference type="eggNOG" id="COG1141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001080">
        <property type="entry name" value="3Fe4S_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010693">
        <property type="entry name" value="Divergent_4Fe-4S_mono-cluster"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06902">
        <property type="entry name" value="Fer4_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00352">
        <property type="entry name" value="3FE4SFRDOXIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Fer4_19" evidence="5">
        <location>
            <begin position="3"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="68" mass="6738" checksum="1FE3D657F679F783" modified="2014-01-22" version="1">MSVHVAADRDLCVGAGNCVLTAPDVFDQDEEGLVAVLDTDPAAAADRVAQAVDLCPAGAITATGGKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Q0</accession>
    <name>U5W3Q0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate--cysteine ligase gCS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27935"/>
    <dbReference type="EnsemblBacteria" id="AGZ43848">
        <property type="protein sequence ID" value="AGZ43848"/>
        <property type="gene ID" value="AFR_27935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5662"/>
    <dbReference type="eggNOG" id="COG2170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1227773_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042398">
        <property type="term" value="P:cellular modified amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="23721" checksum="8648E034DDB69DA7" modified="2014-01-22" version="1">MEEEFLLLDLQSRPEVRHSTVRTVTPPCTGLPELAARLRELRRVSATAAHGTRVHVAVPDRRTASQVCDHLLAWLPLIQALTAGSPLREGADTGHDATGVLPATARPSAAFPAGVEVRVGDVCPTVDDTVLVAGLLRALVATATEYVATGVPAVPVADRVLRAAQRDAAHEGLEGTLLDLRTLASRPAWALLDDLVTAVGPALTRHGDLGFVRDGLARARLTVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3R7</accession>
    <name>U5W3R7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362923.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21405"/>
    <dbReference type="EnsemblBacteria" id="AGZ42551">
        <property type="protein sequence ID" value="AGZ42551"/>
        <property type="gene ID" value="AFR_21405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4343"/>
    <dbReference type="eggNOG" id="COG2154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109854_0_0_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase_6" evidence="1">
        <location>
            <begin position="102"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="22724" checksum="6BEDE26949850A4D" modified="2014-01-22" version="1">MTEPISAGQFHAADGVEDWRSLYHVVTAYFPTGSLRRGIALADRIGQVLGEADEQDVTIDLRHSGVTVSLRRRDADLARRISAAAAGLGLTADPAAVQVVNITLDALTGADVLPFWRAVLGYGQIGDDYLYDPARRGPGFGLQQMTAARPDRNRMHVDVAVPHDQAEARVAAALAAGGHLVSDEHAPRWWTLADPEGNEACIATWLAPRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3U3</accession>
    <name>U5W3U3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362948.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21530"/>
    <dbReference type="EnsemblBacteria" id="AGZ42576">
        <property type="protein sequence ID" value="AGZ42576"/>
        <property type="gene ID" value="AFR_21530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4366"/>
    <dbReference type="eggNOG" id="ENOG5032V9T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153937_0_0_11"/>
    <dbReference type="OrthoDB" id="1880783at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045778">
        <property type="entry name" value="DUF6204"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19707">
        <property type="entry name" value="DUF6204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="119" mass="13120" checksum="ACA23945E15BFD14" modified="2014-01-22" version="1">MSTRTIRVTVRGAFDALTDEQRAGLLERAGEHDVAFAAYTAEGSLTYDIATRPFFTFRFAGTAETEQDIPAVVRHAETAAATYLTEHGYAYKNLTSQAVDMSQVPLGKRGKKEEARRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3W7</accession>
    <name>U5W3W7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27840"/>
    <dbReference type="EnsemblBacteria" id="AGZ43829">
        <property type="protein sequence ID" value="AGZ43829"/>
        <property type="gene ID" value="AFR_27840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5642"/>
    <dbReference type="eggNOG" id="ENOG5033I54">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536850_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="19986" checksum="36C22AA02CE006AB" modified="2014-01-22" version="1">MRGQSSTTGVLTMNEVVARTGTAEKVWRSLAYCAVLIPVALGALVVGRGAAGWWRALRTRLLQAAPVAEGRPGTGAVAVHAVQSLLLGVAALIPLGMQVLMVLRGALYGFVDPGPYDTSWGGPTRGGAWAAHFLISVPLSLAGLALLVGIAAMHQRLTASLDGERRARWLTPVTAGLCVAGGLFLVGWVRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Y7</accession>
    <name>U5W3Y7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560075.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27380"/>
    <dbReference type="EnsemblBacteria" id="AGZ43737">
        <property type="protein sequence ID" value="AGZ43737"/>
        <property type="gene ID" value="AFR_27380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5550"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_2_1_11"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="158"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="347" mass="36260" checksum="58E6F4ED070A52E4" modified="2014-01-22" version="1">MTRVVLVGAGAMGRAWLSAIAENPGVELAGVVDLDLDAARRAAPGLPTGTDVVSVAARAGADAVINVTVPAAHHAVTTAALFAGFPVLGEKPVADTVARALSLTAAAEAGGRLFMVSQSRRWNPQLFTLRDMTAGLGPIATVGTEFFRAPHFGGFREEMAQPLLVDMAIHAFDSARFLLGADPVQVSCQTWNPSWSWFAGDACAAAVFEMTGGARYVYHGSWCAPGPESSWNGSWRVVGQEGTALWDGDHDPVGPSVPAVEAPSGIAGALSVFVEALRTGETPMGEVHGNVLSLAMVEAAVRSSAEGRPVMIDDVLSQAHSEALRDETRPEVRKVLQGWTDVRAALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W415</accession>
    <name>U5W415_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_21905</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21905"/>
    <dbReference type="EnsemblBacteria" id="AGZ42651">
        <property type="protein sequence ID" value="AGZ42651"/>
        <property type="gene ID" value="AFR_21905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4441"/>
    <dbReference type="eggNOG" id="COG4124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038234_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666344" description="GH26 domain-containing protein" evidence="5">
        <location>
            <begin position="33"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="6">
        <location>
            <begin position="241"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="192"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="566" mass="60038" checksum="6CFA435F2BF01B1E" modified="2014-01-22" version="1">MRRKKLQKSVTALAALVAGAGTALVASAPASAVAGPELKLAAGDDTYVSSGRKNAGFGGEDKLAIGRMDGDTKIGFLKFLVPAGVKVTGAKLKLVTVGDIAGRITLSRVADSSWSEKTVTAATAPALGADVASVSPKADAGELTFDLASVVTGPGAYSFALQSSAANAVTRIRSAEGRSGAPALLVSTTATTPAPAETAPAQPSVPVEPPTDYVPDEVVPPSATPIEDEDMPPPATTTPVPTTPAPTTPAPSGGDCVTDKLLVPSCGVLWGAAAGGFTDAPRDQALKDFEKLSGRTASIFHAYHKGDEPFPTKSEMAMARDAAKPRVLLLNWKIAYGSTWAKVAKGEQDARIDKFAARIKSTFPEKFYLVLNHEPENDVVAKSGSGWEAKDFAAMYRHTILRLRAKGVSNAINVMAYMGNEKWMAQSWWKDLYPGDDVVDWMGLDSYVSAEKGYYHFGLFGDLLDRQPTGGGLGWYDWATTKHASKPIMVAEWGVYHRVGKVADKSAGFRSVLPELKKRPGIKAIVYFDTKRDDQGDRDISIDSTKTALAAFKTLAADKIFDVKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4E9</accession>
    <name>U5W4E9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560400.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29010"/>
    <dbReference type="EnsemblBacteria" id="AGZ44063">
        <property type="protein sequence ID" value="AGZ44063"/>
        <property type="gene ID" value="AFR_29010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5878"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_1_11"/>
    <dbReference type="OrthoDB" id="1439833at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="31"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="30520" checksum="C45D054AA6C5AAFA" modified="2014-01-22" version="1">MTSTFEGITHHNAKINGTDLHYVSAGTAGSPILLVHGWPESWWAFRAVVPLLAATHRVFAVDLRGFGDSGTDTGDYDMATMAEDLHQLVAHLGVGPVHVTCQDISGGPVFEFAASHPADTLSFTGVETTLPGYGLEMLADVVNGGSWHVGFLAATGIPELFLAGRERVMLDWAVSVMSAVPGAVTEADLEEFTRTYARPGGWRGTEGLYRSSLTSAGRIRALAESRPLTVPVLAVNGINAPFTERTLRQVAANVTAVTIPDVGHLVAQEAPAAFAATVGEFVDRVDQGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4M4</accession>
    <name>U5W4M4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363397.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23080"/>
    <dbReference type="EnsemblBacteria" id="AGZ42886">
        <property type="protein sequence ID" value="AGZ42886"/>
        <property type="gene ID" value="AFR_23080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4677"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105089_0_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="199" mass="22759" checksum="67EC6F6E771EAD04" modified="2014-01-22" version="1">MGRHKQPEIRDRILDACVDHALAHGLPDRLEPFVAASGTSMRMLIYHFGTRDALLTETLRRARRRQRDFYGELMSPRADEPYLRTLRRMWQAMTGPEGRPFLRMFGWLRESTEQQLWPGFRREATTDWLQPLEDGMRTVGHPELATLVLAVIRGLILDLEATDDVTRADQAFDDFLTALALLTDPALSKQDGSVPMIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Z2</accession>
    <name>U5W4Z2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-(2,3-dihydroxyphenyl)propionate dioxygenase</fullName>
            <ecNumber evidence="2">1.13.11.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mhpB</name>
        <name evidence="2" type="ORF">AFR_23715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.13.11.16" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363601.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23715"/>
    <dbReference type="EnsemblBacteria" id="AGZ43011">
        <property type="protein sequence ID" value="AGZ43011"/>
        <property type="gene ID" value="AFR_23715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23715"/>
    <dbReference type="eggNOG" id="COG3384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078149_0_0_11"/>
    <dbReference type="OrthoDB" id="1728007at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047070">
        <property type="term" value="F:3-carboxyethylcatechol 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LigB" evidence="1">
        <location>
            <begin position="7"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="32776" checksum="659DEB59D65FBA9A" modified="2014-01-22" version="1">MSEIVGFVGMSHSPFATLLPPGSPAEPGGAFLADAARVAAAVAALAPDAVVVIGPDHFHANFYDQMPPFVLGVEEAVGFGDFGSTAGPLPVAGKLAWSLHDKLSSAGFDIALSYALTVDHGVVQSYEMATGRTDIPLVPLVVNTAAPPLPHLERCVALGTALGDALRAADDPSRVLVVASGGLSHWLPSNDPRDPSVVGEKRTNLIHGRRDTRAFAAAREPKVRAMGGNPGARVNAVFDRWFLQQLRGTDLEPVTSLGHERLEEQAGSGGHEIRTWLIGHAAVRKPLIWDSYEPVPEWITGMGIGTTFPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5K5</accession>
    <name>U5W5K5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type 11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363791.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24675"/>
    <dbReference type="EnsemblBacteria" id="AGZ43201">
        <property type="protein sequence ID" value="AGZ43201"/>
        <property type="gene ID" value="AFR_24675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4999"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_2_3_11"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="47"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="27629" checksum="B741A352242B23DE" modified="2014-01-22" version="1">MTSDQPGVTGIFDEAAPAYDRVGVDFFTPMGAELVRRAGIRPGDNVLDVGCGRGAVLVPAAAAAGPAGRVIGTDLAPAMVALTAEAVADLPTVSVQAGDAQAPDFPAGSFDVITAGLVLFFLPDPAAALAAYRELLRPGGRLAFSSFAAYDPLYPEAMKALAAHAVDAPPPRDAGGIFRSEETLRAALTAWAEVQVTEFRQVSRYAGGQQWFDWVDSHGGRQLTRLIPADRRAAAAADAEAVLASTRTPDGSLELTTTIRIVVAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W644</accession>
    <name>U5W644_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lysine exporter protein LysE/YggA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_26055</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26055"/>
    <dbReference type="EnsemblBacteria" id="AGZ43475">
        <property type="protein sequence ID" value="AGZ43475"/>
        <property type="gene ID" value="AFR_26055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5280"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006324">
        <property type="entry name" value="LeuE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="23163" checksum="170CE20678CBF509" modified="2014-01-22" version="1">MPSDRGAAGRRTSPVRSYGVIMTTVTQLVAFCGVVSLGAMSPGPDFAVVVRRSALRGRTEGMAAAAGIATGVFLWAVAAAAGVAALVATVPVVLTTIRYAGAAYLAYLGIRALLAAFRPAPDGLAVAPGTTGGAFRDGLLCNALNPKAAVFFLALLPQFLPAHPTAVDTLTLSLLAVAVTLVWFFTVANLVAAFRRLFARSRVRRTIDGLSGAALLGLGARLALAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6G8</accession>
    <name>U5W6G8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26490"/>
    <dbReference type="EnsemblBacteria" id="AGZ43560">
        <property type="protein sequence ID" value="AGZ43560"/>
        <property type="gene ID" value="AFR_26490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5370"/>
    <dbReference type="eggNOG" id="COG3361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081757_1_0_11"/>
    <dbReference type="OrthoDB" id="1231841at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023375">
        <property type="entry name" value="ADC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018644">
        <property type="entry name" value="DUF2071"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39186">
        <property type="entry name" value="PTHR39186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09844">
        <property type="entry name" value="DUF2071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160104">
        <property type="entry name" value="SSF160104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="27453" checksum="EB2794946E3DB990" modified="2014-01-22" version="1">MRIEDVTPETVRPVRRSLLVQRWHDLAFLHWALPPEVVAPFLPRGTVPDTLDGVTYAGLIGFRMVKLGALRGPGIPYLGTFCETNVRLYSVDGEGRRAVVFLSLDAERLLPVLTAQALLRLPYKWSRMRLEKDRDVLRYTSRRRWPGPRGAASSFSVRVGAPVPEPSAVDHFVTARWGLHTRAWGSSLHLPNEHPRWPLHRAELLDLDDELLTAAGLPAPAGPPVSVLYSPGVPVTFGAPQTLDTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6J5</accession>
    <name>U5W6J5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32580"/>
    <dbReference type="EnsemblBacteria" id="AGZ44774">
        <property type="protein sequence ID" value="AGZ44774"/>
        <property type="gene ID" value="AFR_32580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6593"/>
    <dbReference type="eggNOG" id="ENOG5032Z5X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2366533_0_0_11"/>
    <dbReference type="OrthoDB" id="1994866at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="95" mass="10442" checksum="8C95003F58E647DE" modified="2014-01-22" version="1">MAVPATVQLRYLLCAATAWNDTFFKDPVVTDALATNGVPEEDRDWDCLTHCFAAARSEDATTSAPGNLLMTRYQHHTFSVLPANWPGIFSAVRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6N1</accession>
    <name>U5W6N1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44662">
        <property type="protein sequence ID" value="AGZ44662"/>
        <property type="gene ID" value="AFR_32010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6480"/>
    <dbReference type="HOGENOM" id="CLU_1736623_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="150" mass="15328" checksum="26CF7E597974F078" modified="2014-01-22" version="1">MLPADATEGVAKPGAWQPLPVQPPEAEPSASPTRGPAWLLAVLDFIERATGRTTSLREHLTMFIVYVLMILLTVAVVIGGAVVLVVNVISIGTWPGITSIVVLAASAGGAVFVKRAQRDATPPATGPTSGSTATESTSPDATEAGESPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W713</accession>
    <name>U5W713_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_27570</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27570"/>
    <dbReference type="EnsemblBacteria" id="AGZ43775">
        <property type="protein sequence ID" value="AGZ43775"/>
        <property type="gene ID" value="AFR_27570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5588"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="72"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="29548" checksum="BF58C72CFEFC0FEE" modified="2014-01-22" version="1">MFSAVVGLVVLVALWEGYKAVGNPDGTVLLGVRVLPRADDLSMPHVWDMLQRLGRPELTGGRPVWQVVLSASLFTLGITAVGFVAGAVIGLLLAVAMQRFRIVERGLLPYVILSQTVPLVALAPLIAGWGGTIWPEWMTVALIAAYLAFFPVAVGMLRGLQSPQAAGVELMRSYAAGWWRTLLKLRFPASLPYLFPALRLAGAAAVVGAVVGEISTGTRGGIGRLIIEYSREATSDPAKVYTAMLGAALLGLVVAALVSLLELPLMRHRRRVSVVTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W791</accession>
    <name>U5W791_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27995"/>
    <dbReference type="EnsemblBacteria" id="AGZ43860">
        <property type="protein sequence ID" value="AGZ43860"/>
        <property type="gene ID" value="AFR_27995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5675"/>
    <dbReference type="eggNOG" id="COG3599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091967_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007793">
        <property type="entry name" value="DivIVA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35794">
        <property type="entry name" value="PTHR35794"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="28406" checksum="4699C8506F59C2DA" modified="2014-01-22" version="1">MTDNRMKDRVKVMLGTSPDGATSEHALVSTTPVPGLPAGLPNQALQVLTMAQRTAEEHVATAHQQADKIRTDALAAAEQIARDAQIHAHNVRREADKVLFDARAVSEQNARDARAAAEAAQRNADKIVADAQARADSIAAEAHSNAEHLRGQAQQRFDDVVGSLGTKREALQEQIEALEQFDREYRGRLTSFMQGQLRALWVDQPQVTGELENPEDSRPGRHGTVTADEPTPALRQEAVTEPQPQPQPQQDDASPESEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7K5</accession>
    <name>U5W7K5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_34180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561431.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34180"/>
    <dbReference type="EnsemblBacteria" id="AGZ45094">
        <property type="protein sequence ID" value="AGZ45094"/>
        <property type="gene ID" value="AFR_34180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6916"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="32957" checksum="90469CF1B5B87062" modified="2014-01-22" version="1">MASVIEVQHLHKRYGDTVAVDDLSLTVEEGEILGILGRNGAGKTTTVECVEGLRAPDRGQITVCGFDPRRDRAEVTRLLGVQLQDGRLPDRLRVAEALELYSSFYPRPADWRGLMDSLGLTAKAQTRYAKLSGGQKQRLSIALALVGNPRVAILDELTTGLDPQARRETWDLIEDVRARGVTIVLVTHFMAEAERLCDRVALIDAGRVVTVDTPAGLAAQAHLGQRIQFRPSRPFDDSLLLGLPDVTGVTRRGDLVIVDGTDNALTAVTAVLGRNQITAERLRVEQASLEDAFVHLTTKRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8E3</accession>
    <name>U5W8E3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560533.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29675"/>
    <dbReference type="EnsemblBacteria" id="AGZ44196">
        <property type="protein sequence ID" value="AGZ44196"/>
        <property type="gene ID" value="AFR_29675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6015"/>
    <dbReference type="eggNOG" id="COG0115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020844_3_0_11"/>
    <dbReference type="OrthoDB" id="1275805at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="300" mass="31846" checksum="C901881C918AD540" modified="2014-01-22" version="1">MLQSYDERNAGIKYWVNGVLRHRDEPGISPFDSAVQGGDAVWEGLRVYQGSIFGLEAHLARLRRSATALNFADIPTDDEIIAAVVATLQANNMVDGVHIRLTLTRGVKVTSGMDPRLNQAGPTLIVLAEHKAPVYDTSGLSLITASVRRPGPDVLDPKIHHANLLNSILAKIEATVAGADDALMLDGRGFVAETNATHLFAVINGALVTPTTAACPEGITRQTVLELAEAAGIAAEVRDMSLAEMYSADEVFCTGTMGEIAGVTRIDGRTIGSGQTGELTARIAALYQQHARTHGTQVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8N1</accession>
    <name>U5W8N1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase domain-containing protein hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560633.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30180"/>
    <dbReference type="EnsemblBacteria" id="AGZ44296">
        <property type="protein sequence ID" value="AGZ44296"/>
        <property type="gene ID" value="AFR_30180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6111"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_0_11"/>
    <dbReference type="OrthoDB" id="1204073at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="23570" checksum="C672A08FF288DA52" modified="2014-01-22" version="1">MPPPPLTCVLFDFAWTLFAGDPDTWVRGAAQAIGRPVADGEPAAISAAYMDRLRTTATDPAHLARDLDPSVYDAAISVVLTGIPGVTPDFAAALAATHMTSLAPYADVASTFKTLKTLGVRIGVVSNIGCDLRPAFTRNGLDGFVDAFVLSYEVGFVKPDPAIWKAALHALRADPAETVMVGDHPAGDGGAVVAGIAALVLPQVASPLEPRGLDHVVALVHGRARKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W947</accession>
    <name>U5W947_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560796.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31005"/>
    <dbReference type="EnsemblBacteria" id="AGZ44461">
        <property type="protein sequence ID" value="AGZ44461"/>
        <property type="gene ID" value="AFR_31005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6276"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_11"/>
    <dbReference type="OrthoDB" id="1261526at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26098" checksum="341026DDCDC3C33B" modified="2014-01-22" version="1">MTGIRDKVIAITGAGSGIGEATALLLAERGAKVVLAGRREERLAALAERITAAGGQAAYAVTDVRRRQDVADLVALAGERFGRLDVLISNAGYGATALLDDLRVEEWEDLVDINIKGVLYGIAAALPVFRKQGTGHFVHTLSTSGLTITPTMAVYAGTKNAVRTISEGLRQESGGDVRVTTVSPGFVRTDFVDHTADPEVRDRLAVQRDRFAIPPEAIARAIAFAVEQPDEVDVNDIVVRPAAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W967</accession>
    <name>U5W967_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_31130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560820.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31130"/>
    <dbReference type="EnsemblBacteria" id="AGZ44486">
        <property type="protein sequence ID" value="AGZ44486"/>
        <property type="gene ID" value="AFR_31130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6301"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_44_3_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041347">
        <property type="entry name" value="MftR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17754">
        <property type="entry name" value="TetR_C_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="22321" checksum="A34B785BB4F0FB7C" modified="2014-01-22" version="1">MGHREDLLAGAKRCLYDKGYARTTARDIVAASGTNLGSIGYHYGSTAGLMNAAMLSAIEDWGDAIGEALSAEITDESDDPMVRYWRRVIGTIGTHRELWLASIEAMLQSEHAPELKAQIAAGLEQGRSGLAAVLLGREEDSLDERTIRSVGSVSLALMSGVMTQWLTDPEHAPSAEQIAEGVRALSLSPALPPSMNNPWPPDAVPSRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9B9</accession>
    <name>U5W9B9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562138.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37745"/>
    <dbReference type="EnsemblBacteria" id="AGZ45803">
        <property type="protein sequence ID" value="AGZ45803"/>
        <property type="gene ID" value="AFR_37745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7633"/>
    <dbReference type="eggNOG" id="COG5563">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_693772_0_0_11"/>
    <dbReference type="OrthoDB" id="1605675at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="407" mass="43090" checksum="C3E2FA4BE2CAED1C" modified="2014-01-22" version="1">MTKHPNDDDRYAVALLRPLASEPSGPARLDVSRAMADGRRRRRSRWWAGGTAVVALTATTAAGGTLAFSAIGRPSPKPPVTIATPTPSVTAAAPPAGPKNCKVTRLPTDGAKKALVTAGDPSGHWLVGRTYPQGPSRPFVVWKDGKLVATGETSGEDQSLEDINSKGVAVGYSFDPGVRPYSYRNGKVTAMAGGEAVPTAINDAGVVVGAVGPLYEGRPVRWKSVTAEPDRLPVPAGTEYGTATDIDEDGTILGTVSAKNKEATGYLWLADGSTRRMPLPVVDGVKSTMFWPAAIRNGVVVGRSVTDAGDSRTFAYFRYRLDTGRYERLPEGSGDPARVAANGWVLGEAQRPVVTTDKGVTTMLPKYAKAKGRQDYLVSSFSDDGKVVGGYAIGDDIQNQPLMWRCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Z8</accession>
    <name>U5W9Z8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562298.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38550"/>
    <dbReference type="EnsemblBacteria" id="AGZ45964">
        <property type="protein sequence ID" value="AGZ45964"/>
        <property type="gene ID" value="AFR_38550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7800"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_10_0_11"/>
    <dbReference type="OrthoDB" id="1900922at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="15275" checksum="E12D61726FE1236E" modified="2014-01-22" version="1">MTLQLSHCFLVVHDYEEALAFYRDVVGLEVRNDVSFEDNRWLTLGAPGQPGVEIGLEIPGYYPHTRPEDKQATADLLAKGLLPGVIFRTSSCDETFEKVRAAGADVLQEPIDQPYGVRDCAFRDPSGNMVRFSQLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA07</accession>
    <name>U5WA07_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Multidrug ABC transporter ATPase/permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_37990</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37990"/>
    <dbReference type="EnsemblBacteria" id="AGZ45852">
        <property type="protein sequence ID" value="AGZ45852"/>
        <property type="gene ID" value="AFR_37990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7683"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="388"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="422"/>
            <end position="429"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="653" mass="70336" checksum="DAA77C448095EEEC" modified="2014-01-22" version="1">MTFVDTSGVVMVDQSTDDAVNRSVWAEFTGGRQARWAQRRAMLRLLPAGGPVLVGSAVVLQLLAGLLPIGFVLATASVIAGVPDAVTGGVDSPAWQQLRDHLITAAALFFATQLLLPVQAFLGDVLRRRIDDQVRDQLMADSFVGPGIAVLEDPEVLDQAADGVNMLRMAAWSPGAACAGFIALLSRYLQTITAAVVVAVVFEWWAGVAVLLAGLVIRFGYRIGLSVFGRIYTLHQRVRRRRFYFRDLLFTSVAAKEIRIFGLLGWVGDRYATAFMDSSVPIWRQRRRIFYGPYVLYTLTSFVLLGGALVVTARASATGVLGIGAFMIVMQASLSSIRIGAFIAESDVQTEYGMNAQQALIRLGELTAAAADPGGTEKPSAELPIREIRFEQVTFRYAPDAPPVLDGLDLVIPAGGSLAIVGLNGAGKTTLVKLLARLYEPESGRITADGVDIRTFDGAAWQRKVAAIFQDFVHFELPVRDNVGFGAGGLRDDAPIEEALRRAGALDFVTALPAGLDTPLSRQYTDGTDLSGGQWQRIAIARALIAVEGGARVLVLDEPTASLDARAEVAFFERFLDLTSGATSLVISHRFSTVRRADRIAVLEHGRVTESGTHTELLSAGGRYAELFLLQAARFTEDTPAEEPELLGESADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA39</accession>
    <name>U5WA39_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32960"/>
    <dbReference type="EnsemblBacteria" id="AGZ44850">
        <property type="protein sequence ID" value="AGZ44850"/>
        <property type="gene ID" value="AFR_32960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6670"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087580_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="14"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="21500" checksum="C60F11632E11E14C" modified="2014-01-22" version="1">MESNPARRVVTDPLALRALAHPLRLKLWGLVGREGQLTAADAARQLGVSQALASHHLRQLAKYGYVERGSAGDNRERPWQITATSNDFVGDNSQEGQESAELLDRYVAEQAVVQLADWQQRRADEDPEWSEHSGVAQSLLYLTVEEFAELTRAWHALTSELAARRPLGDASKRGPGVKPVNFTLVAAPLRPTDSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAC0</accession>
    <name>U5WAC0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561277.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33410"/>
    <dbReference type="EnsemblBacteria" id="AGZ44940">
        <property type="protein sequence ID" value="AGZ44940"/>
        <property type="gene ID" value="AFR_33410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6762"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_43_3_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="39"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="26100" checksum="8A13B272B66BF7DC" modified="2014-01-22" version="1">MPEVISQDGTTIVYDRSGAGPALILIDGAMCHRSAGPMQALATLLTDHFTVYTYDRRGRGESGDTAPYAPEREAEDVQALIAEAGGTAYLYAMSSGGAVALRTPGAAGVVLYEPPFGLGSREYTDRLRELLAAGSNGDAVALFMRNVGVPEPVVTGMRNQPGFASVEAIAPTLAYDDELLTEMPADQGVPLLVVSGSASPESLQKAARMTAEAFPGADHRTLEGQTHDVQPAALAPVLIDFLRNAGAGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAV9</accession>
    <name>U5WAV9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">HTH-type transcriptional regulator betI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_39390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562466.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39390"/>
    <dbReference type="EnsemblBacteria" id="AGZ46132">
        <property type="protein sequence ID" value="AGZ46132"/>
        <property type="gene ID" value="AFR_39390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7969"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_40_0_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="190" mass="20209" checksum="3CDABE274B86836F" modified="2014-01-22" version="1">MAKETYHHGDLRRALLAAAAEAITESGPAALSLRDLARRVGVSHAAPTHHFGDKKGLLTALATEGFERLSAALTETRAATGSFLELGVAYVRFATTHPAHFQVMWRPDLFHEDDEALQAAQERAGDALYGGVADLPDGWAGNGASSVEEAGLAAWSLAHGFATLWLSGAIPRTTETPDEAARAVLRQLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAY5</accession>
    <name>U5WAY5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562491.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39515"/>
    <dbReference type="EnsemblBacteria" id="AGZ46157">
        <property type="protein sequence ID" value="AGZ46157"/>
        <property type="gene ID" value="AFR_39515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7994"/>
    <dbReference type="eggNOG" id="ENOG502ZBFQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965182_0_0_11"/>
    <dbReference type="OrthoDB" id="1199828at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="33189" checksum="A664DCFA77645C23" modified="2014-01-22" version="1">MTSQSPEEGSTVKEEKAVVEDGLSGRELWDEVRIAPIEIAMPGGVGLTLRAYRLASELTPTEIQTDEDDPFDARERRAAARRAEEEGVIVDDEFSALVAEGETDSKADRKSDNKVVTEDGEVVEEPDAADFADEPDETEAEEADEEVPIFLSHKGKLLTFKTPESLVSFIRSGAPHDLAQLDTWATVAERVQSSDIDPAADDRYELDLVVENLRGGHDTWDLPLLIAAGEVARDLGHGLRLKPVILALAPGSPLDDLDESLRSAESGGMGGFFGRRKLRKIGAQQASLGWRSIIGKISAAVDWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB05</accession>
    <name>U5WB05_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kinesin light chain 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561492.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34485"/>
    <dbReference type="EnsemblBacteria" id="AGZ45155">
        <property type="protein sequence ID" value="AGZ45155"/>
        <property type="gene ID" value="AFR_34485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6977"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762129_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="363" mass="40954" checksum="1C9BC601099D7DB3" modified="2014-01-22" version="1">MLDFDHRMPRRPEVLAVVRGLEETIAAQGRLFGPDDPRTVGSRLRLVEAYEDLAIAGDDRDLDALMEAELAKAVDSRLRTLGSDHPDTLDVQFDLTLLRFRRAVQYGLPGQDELVDEFRSIGDTSARTLGAAHRMTLKAWAQCAFSSGPERSALKAKIVQGWEELLADHEQRLGPDDPETLETMERLLAQYRDDHPEAARRLGERLTAGWGRVAAARTAELGPVHPETATARDQHLRLVDEWITPGAGEHLFEELVADHLRLLGPDHPRTLHTQLVLLRYWKGLPAYATPETLALAEQLLDRFLEVLGPHHDDFLMLRYHLMSHHAIKGSLEAALDLKRRYPTPDDEEQDDNDEDGATHLETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBF0</accession>
    <name>U5WBF0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FtsX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_35075</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561607.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35075"/>
    <dbReference type="EnsemblBacteria" id="AGZ45271">
        <property type="protein sequence ID" value="AGZ45271"/>
        <property type="gene ID" value="AFR_35075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7097"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_360826_0_0_11"/>
    <dbReference type="OrthoDB" id="324902at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="614"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="655"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="266"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="743" mass="74278" checksum="38977FC0276D27BD" modified="2014-01-22" version="1">MSGLGRVIRSGVRRRRVQTVVIGLVAAAAVTASVLGAGLLVASRAPFDDAFSAQRGAHLTVQADPDRVTEAQLTASASAPGVAAASGPYPVMGLDFRGELSLTVVVRADPGGPVDRVELAAGRWPAGPTEIVLGGGALRMPVGVQMTTAGGTVFTVVGTARSVSATAAAWVLPSAQLGPPDGYQMLYRFGAASTPAEVDAGRAAVAATLPAGAVTAARSWLDVRQRAVEDAAVFVPFLVAFALLGIVMAVLVVGTVIAGTVGAATRRIGVLKALGCTPAGIVRAYVAQALLPAAVGAVAGMVVGNLIALPVLAETEEVYGSANTTIAWWVTALAAGGVLAVVAVTAWAAALRAGRLRTVDAIAVGRASGPARGRWAARLAARLPVSRPVGLGLARPFARPLRTAAILLAVASGTAAVTFATGLGTSLVRIQTASEQNTADVMVEGNGGPGVLAAIEARDGTRAAYGIGQAPGSVAGFADPLSVNALTGDGSWDGFEMVSGRWFAAPGEAVAGAPFLKAAGARVGDTVTVQVAGRPVALRVVGEVFETGTGVFTSAATVPGLSPGEFRIALTDGTDAGSYAEALTGVLPAGWTAHPNGGEIDPLLVVVQGLTATLTLLLIAVAALGVLNMLILDLRDRVHDLGVHKALGMTPRQTIVMVVASVAGIGVAGGLLGVPVGVVMQRLVVPAMAASGGLHVPETLLDVYGPVLLIVLALGGPATALLGALLPAGWAARTRTAVALRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBH3</accession>
    <name>U5WBH3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar motor switch protein FliG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fliG</name>
        <name evidence="13" type="ORF">AFR_40440</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562674.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40440"/>
    <dbReference type="EnsemblBacteria" id="AGZ46342">
        <property type="protein sequence ID" value="AGZ46342"/>
        <property type="gene ID" value="AFR_40440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8185"/>
    <dbReference type="eggNOG" id="COG1536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047835_1_1_11"/>
    <dbReference type="OrthoDB" id="847822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000090">
        <property type="entry name" value="Flg_Motor_Flig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023087">
        <property type="entry name" value="Flg_Motor_Flig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011002">
        <property type="entry name" value="FliG_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032779">
        <property type="entry name" value="FliG_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028263">
        <property type="entry name" value="FliG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30534">
        <property type="entry name" value="PTHR30534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01706">
        <property type="entry name" value="FliG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14841">
        <property type="entry name" value="FliG_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14842">
        <property type="entry name" value="FliG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003161">
        <property type="entry name" value="FliG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00954">
        <property type="entry name" value="FLGMOTORFLIG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48029">
        <property type="entry name" value="SSF48029"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00207">
        <property type="entry name" value="fliG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliG_N" evidence="12">
        <location>
            <begin position="11"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="FliG_M" evidence="11">
        <location>
            <begin position="120"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="FliG_C" evidence="10">
        <location>
            <begin position="223"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ46342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="341" mass="37681" checksum="9A73F9FBB3932CA5" modified="2014-01-22" version="1">MTTPALSTTSMTGLRKAAILLVRMGKEYSTRVLASMNENEVEELSAEIARLGKLEPDVVGDVIDEFYAMATTKHAGAGGMAYARELLEASLGTERAQLILDRLQASMTDMPFNFLSHADPRQLLSYVQYEHPQTIALVLAHIPSALASSILSGLAPEVQSDVAHRIAVMDRTSPDVIRQVELALQRKLSTVLQPDELSTVGGLDPLVDIINRADRTTERLILEALEARNPEIAEEIRRRMFMFEDIVSLDDRSIQLVLRQVEPADLATALKGVGEDVRDKVTRNLSERGRENLLEEMDLMGPVKVKMVEEAQQKIVSVIRSLEDSGQIEIQRGGEADELIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBP9</accession>
    <name>U5WBP9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_35870</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35870"/>
    <dbReference type="EnsemblBacteria" id="AGZ45430">
        <property type="protein sequence ID" value="AGZ45430"/>
        <property type="gene ID" value="AFR_35870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7260"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="69"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="29815" checksum="4834B6729E69D3DD" modified="2014-01-22" version="1">MRTLTTRFAGYTILVFFSLLFLYPFVIQIGNSFKTEADAAANPLSPLAHPFTTDSVARIFDGTGFPTWLGNSVLVTVVVTLTRVLFNSMAGYALSRLQFRGRKVLFAGVIALMAVPPVVLLIPKFLVLNQLGMYNSYAGLIVPIMVDAAGVFIMKQFFDSVPVSIEEAARIDGAGVFRTFWSVVLPSARPALITLTILAFQGSWNEFPHTVVAVQDPSLFTLPRGLADLVSGSLGAGNQYPLKLGAALLATIPVAIIFVVFQRYFVRGATEGADKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBZ8</accession>
    <name>U5WBZ8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome maturation factor RimM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 5" type="primary">rimM</name>
        <name evidence="5" type="ORF">AFR_36320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Binds ribosomal protein S19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The PRC barrel domain binds ribosomal protein S19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RimM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561856.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36320"/>
    <dbReference type="EnsemblBacteria" id="AGZ45520">
        <property type="protein sequence ID" value="AGZ45520"/>
        <property type="gene ID" value="AFR_36320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7350"/>
    <dbReference type="eggNOG" id="COG0806">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077636_0_0_11"/>
    <dbReference type="OrthoDB" id="1497415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="16S_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RimM" evidence="3">
        <location>
            <begin position="4"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="PRC" evidence="4">
        <location>
            <begin position="113"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="19646" checksum="3C8FB7B0BB9C1611" modified="2014-01-22" version="1">MLLVVGQIGKPHGIRGEVLVTVRTDDPEARFAAGSAFTTEVPRDRRVSAGPAGAAAAGVPYRVPDKLTLEAARFHQGKVIAQFEGVLDRNTAEALRGVLLQVDSSSVTAPDDPDEFLDHQLVGLSVVSVDGSVLGTVGRIDHAPASDLIVLEKAGGGTALIPFVSQMVPTVDLAGARVIVDLPEGLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCE2</accession>
    <name>U5WCE2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prephenate dehydrogenase</fullName>
            <ecNumber evidence="3">1.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562968.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41915"/>
    <dbReference type="EnsemblBacteria" id="AGZ46637">
        <property type="protein sequence ID" value="AGZ46637"/>
        <property type="gene ID" value="AFR_41915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8481"/>
    <dbReference type="eggNOG" id="COG0287">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055968_1_0_11"/>
    <dbReference type="OrthoDB" id="533829at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666297" evidence="1">
        <location>
            <begin position="21"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="330" mass="33717" checksum="9601CE183AF95166" modified="2014-01-22" version="1">MKVAVIGLGLIGGSLLRALAAHGHTVTGYDLEAETREAARALAKAADAPRVADPETRTIAVKGAWHVADSVAEAAAGAEVVVLAVPLPALPDVLAELEGHPGLITDVTSVKGPVRDLLKGHRFVGGHPMAGKESSSFRASDPSLFDGCAWVLCLEPGTTDVADWLSLAALFLPLGVRIVPVTAAEHDRAVAQISHVPHLLAAALAALLEGNPLAATLGAGSFRDGTRVAGTRPELIAAMCGGNADAVREVLAGVIGDLERYDKDLHGAEAITTLTERLRPATELRQAWPPVPGSPVELPVDAGALLALGRAGGWVTAVDGDSISGITAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD25</accession>
    <name>U5WD25_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_37960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37960"/>
    <dbReference type="EnsemblBacteria" id="AGZ45846">
        <property type="protein sequence ID" value="AGZ45846"/>
        <property type="gene ID" value="AFR_37960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7677"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="31846" checksum="4AF40EEE0EFE1A16" modified="2014-01-22" version="1">MPTGSEEIVVSNLTKHYKALRAVDNLSFIVHSGRVTGFLGPNGAGKTTTLRMILGLVTPTSGTATFAGVRYVDLDQPIRHVGAVLEASSAHKGRSGINHLRMICAAAGLPRQAADEALARVGLTPAAKRKFKGYSLGMKQRLGIAAALLGNPRVLILDEPANGLDPEGIRWLRDLLKALAAEGRTVLVSSHLLSEMQQLADDVVIIAAGQLVRQGPVGEVLGSMTGGGQVRVRTPQTAALTAALAAEQLSATTLPDEALLVSGADAARVGHIAFTAGVELHELTGERADLEQVFLQLTAGKAGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD94</accession>
    <name>U5WD94_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562256.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38335"/>
    <dbReference type="EnsemblBacteria" id="AGZ45921">
        <property type="protein sequence ID" value="AGZ45921"/>
        <property type="gene ID" value="AFR_38335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7755"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042635_0_0_11"/>
    <dbReference type="OrthoDB" id="1408716at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="383" mass="40334" checksum="25948686DCD21C89" modified="2014-01-22" version="1">MSRTPLPRPTLLPGLPRIWRTPHTLQLGLDPPRAVLLDLPEPRAAGLLDLLDGSRSERLALAEAATFGMRPDEARTLLDTLHTAGLVVPAQSLFPPAMPERLTGEAAALAYSATTTSPARTLRRRASARVVVTGRTRLAPAVAVALATAGIGHLHPDLTGPVEAHELPGSPLTPADLGHPRSQATASALRRAAPTTETRPVRRGAASLVIHLGEDHPTALSAISHAHRRRPLLTASIREGKVLVGPLVPPSTSPCLNCITLHRRDREPTWPLDDPWSSPSGPFKAGSLLLESASPDAGEVGSGIGPGRAGPAAVVPPEPCATATLLAAVAYITAEALTFVDGNTPETLGAEVEISRPGTFRRRTWSPHPACPCTRRSSLPRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEB5</accession>
    <name>U5WEB5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562608.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40110"/>
    <dbReference type="EnsemblBacteria" id="AGZ46276">
        <property type="protein sequence ID" value="AGZ46276"/>
        <property type="gene ID" value="AFR_40110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8117"/>
    <dbReference type="eggNOG" id="COG3427">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123373_0_0_11"/>
    <dbReference type="OrthoDB" id="1719670at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="17831" checksum="E49028EEC855582C" modified="2014-01-22" version="1">MSETGPINNLGDAARPGTGEVTATVIVNAPAPQVFAAFMNWERQSDWIPLTKVRVVEGDGGEGSLIEAITGVGRAGLRDEMRVVRVDAPYELRVVHCGKVLRGPGSMRCTAMGGDRTQVVMHEWFHLPGGAATKVAWPLLWPGSKLSLKASLKKFGRLVEEGRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEN1</accession>
    <name>U5WEN1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_40760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40760"/>
    <dbReference type="EnsemblBacteria" id="AGZ46406">
        <property type="protein sequence ID" value="AGZ46406"/>
        <property type="gene ID" value="AFR_40760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8251"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033995_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666526" evidence="6">
        <location>
            <begin position="24"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="544" mass="55790" checksum="DCBF148B919547BD" modified="2014-01-22" version="1">MAFACVISFMGIGLVDPILPALATDLHASPSQVSLLFTSYLVVTAVAMLVVGAVASRIGPKRTLVVGLSIIVVFAALAGTSGTVGQIVGFRAGWGLGNALFIATSLAVIVASASGGFAGAIILYETALGLGIAVGPLLGGELGSISWRGPFYGVAVLMALAMVATIVFVPALPKPEKPTSITAPLKALRHRGLLTMGIMALLYNWGFFTMLGYAPYPMELDAHKLGLVFTAWGLLVAAFSVFFAPRLQARFGTAKVLYANLLALAVVMVVIAAGVSTPATVIAAVIVSGAFIGINNTLTTQAVMLVAPVERSVASSAYGFVRFIGGGLAPFVAGKLADRFDLSVPFYLGGVAFLLAIVVLSTGHKLVTAAEQDESPLQPVGALTPADARPVVVAVGAAPDAPEIVAAAAAIARASGSPLEVVRVRETVVIEELAIEPEDEQDARAAVEGHLRRLADHGIAATGLLLTSVGDHAAAGRALARHAEDVQARTVAVGRSPRGPAVQFADGSFTSALTHSTTRTVVLVTPDETPHPLTAATLHELRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEZ7</accession>
    <name>U5WEZ7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">def</name>
        <name evidence="3" type="ORF">AFR_41335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41335"/>
    <dbReference type="EnsemblBacteria" id="AGZ46521">
        <property type="protein sequence ID" value="AGZ46521"/>
        <property type="gene ID" value="AFR_41335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8365"/>
    <dbReference type="eggNOG" id="COG0242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061901_4_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="19259" checksum="9E5DE38897DC233F" modified="2014-01-22" version="1">MSDALADWKPDVLGVDGKVLPVVTAPASVLSTVGADVDPLDPEVVQLAADLVATMRVSPGCVGLAAPQVGVGARVFAVDVTQHPKTATNHGTFVLCNARVVEASRWRPGREGCMSVPDLTGDVKRASRLVVEAVLPGTGEAVLLTTDAFEARALQHEIDHCDGKLFLDRVAGAHAVYQRKVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFM3</accession>
    <name>U5WFM3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin reductase</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_42930</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563170.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42930"/>
    <dbReference type="EnsemblBacteria" id="AGZ46840">
        <property type="protein sequence ID" value="AGZ46840"/>
        <property type="gene ID" value="AFR_42930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8685"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_1_11"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="5">
        <location>
            <begin position="6"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="319" mass="34376" checksum="6ABF1DA6D467FBE6" modified="2014-01-22" version="1">MDEVRNLIIIGSGPSGYTAALYAARANLKPLVIEGVQSGGALMTTTEVENFPGHPDGIMGPELMDNMRKQAEKFGAEFITDDVTRVELGDRPTEAGTEGLKTVWVGEKEYFARAVILSTGSAWRPLGVPGEQELLGHGVSSCATCDGFFFRNQHIIVVGGGDSAMEEATFLTRFAETVTIVHRRDSFRASKVMQLRAQSNPKIKVEWNSVVEEILGDDGKVSGARLRNLQTGETKVLDVTGVFVAIGHDPRSELFKGQVEMDDEGYVKVDAPSTRTNLSGVFAAGDLVDHTYRQAITASGTGCAAALDAERFIASFVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFN5</accession>
    <name>U5WFN5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42410"/>
    <dbReference type="EnsemblBacteria" id="AGZ46736">
        <property type="protein sequence ID" value="AGZ46736"/>
        <property type="gene ID" value="AFR_42410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8583"/>
    <dbReference type="eggNOG" id="ENOG502Z7WX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018249_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039535">
        <property type="entry name" value="ASST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14269">
        <property type="entry name" value="Arylsulfotran_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="506" mass="54670" checksum="1D5A70E104E20AE2" modified="2014-01-22" version="1">MTETKISRRNVLLGVGGAAVFTAAGGGAGYAIANTDGKSPEASAAAAPAPAKFRSRPDLRTLPDVTITTPAAATAPGYIYLTPASGTGLWGPLIVDDKGSPVWFRKVPDPATVAIDFKVQQYQNKPVLTWWEGTIGGTGGQGVGQGEFVITDANYREITRVRAAGSEQADQHDFVITPNDTALFWIYDPIPYDLTSAGGPADGVLHDGVLQEIDIRTGRRLFEWRARDHVSLAESYAPLPGGESAHLPYDYFHANSVGLDADGNILISARHTWTTYKINKRTGDVMWRIGGKKSDFTVDEKSKFSWQHDFRRRRDGSYSVFDNGAGVTKEREESRGLIFKIDETARTVSFVSEFLHPSKLSAPTQGSFRELADGGSFIGWGQMPYFTEYAPDGTVRLDGHLPLDNQSYRAYKAEWPGVPLDQPALGLHVDGSNVIVSVSWNGSTKVSKWRARAGGQPGALTQSAEADRTGFETTLTLPGTPEYVVAEALDDTGKVLGTSSAIPVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNI8</accession>
    <name>U5VNI8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">MutT/nudix family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_00365</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00365"/>
    <dbReference type="EnsemblBacteria" id="AGZ38362">
        <property type="protein sequence ID" value="AGZ38362"/>
        <property type="gene ID" value="AFR_00365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.75"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1544344_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="40"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="18905" checksum="53E8AA247BAA579A" modified="2014-01-22" version="1">MSFEELRRQSLGAALGDIRRARVAFDDVAAWVAVVGDSPEDPLGADVWVFDAGLSQVLLVQHPWRGWVAPGGQVEPGETPRDAARRELFEETGVEAELFDQPAGVNVRSYHPERPASLSLSYAAIVDSAVPLVGEPGQPAAWMKLDQSWESCFPEDVSRIRQHAEWLAGGGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNN3</accession>
    <name>U5VNN3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38404">
        <property type="protein sequence ID" value="AGZ38404"/>
        <property type="gene ID" value="AFR_00575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.117"/>
    <dbReference type="eggNOG" id="ENOG50349XD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075071_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="30863" checksum="B6A6FA0E93674075" modified="2014-01-22" version="1">MSAPRSRPTVVTVSSYLLYLASAASIIAGVLSLTTVGTTRDVYTELYAGTENSGIESVIVAVSVVGVVINILFAAALAILAIYNNRGKQAARITTWVVGGIFLCCNGFGLLGTAATSGMNLDTGTTSGPSASDIEAELADRLPGWFQPISTTLTVIVVLTLLGAVILLMLPAANEFFRKPAVAWDPLNPYPGMPGQQPPYPGQPYPGQPYPGQSPYPTYPGQPAGYPAYPGSVSGPTTPPAAPPPSGEPTPPPASGPEADPHTGSVQPTDPWSAPSPPPSQTPPSPPSEEDKPGPPPANPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNN9</accession>
    <name>U5VNN9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_00600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the DedA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357353.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00600"/>
    <dbReference type="EnsemblBacteria" id="AGZ38409">
        <property type="protein sequence ID" value="AGZ38409"/>
        <property type="gene ID" value="AFR_00600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.121"/>
    <dbReference type="eggNOG" id="COG0586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032818">
        <property type="entry name" value="DedA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30353">
        <property type="entry name" value="PTHR30353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="29752" checksum="DA4B400756E00E1C" modified="2014-01-22" version="1">MAAAGTLLLVDIRALSEQLALNPMDPKDLLQTFGLIGVWAILFAETGLLVGFFFPGDSLLFLAGVAASPVADSIFGDGTRMSLVGLLIGAPLCAIVGAQLGHLLGAKYGRRMFERPDSKLFKREYVEKAEFYFEKFGPAKAVVLARFIPIVRTFLNPVAGTLGMPARTFFLWNVVGAILWCDGVILVGYLLAQQIYDAIGDKIDHYILPVVALIVLISVLPILIEMLRERKAKKNAAAEPGPATAIGVVAAATVAGMAEAITDHEDDHSDHKPRHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPL6</accession>
    <name>U5VPL6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_02275</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357687.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02275"/>
    <dbReference type="EnsemblBacteria" id="AGZ38744">
        <property type="protein sequence ID" value="AGZ38744"/>
        <property type="gene ID" value="AFR_02275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.460"/>
    <dbReference type="eggNOG" id="COG4942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073856_0_0_11"/>
    <dbReference type="OrthoDB" id="1180964at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665197" evidence="3">
        <location>
            <begin position="32"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="143"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="37053" checksum="9B9E23A24B46B7B3" modified="2014-01-22" version="1">MTARPRRWLVLALAPLVAVTMLLVPASPASADPDDDKKSSTADDGDDDPLLNDVLDSTGRRFLAAKSAVAKSTKTQLGLALKVKNAETRRDSLLPEVGAVAAQQYRTGGISTMGFLLNTDGPDGFLKKAVSLEEINAINDGKLHELYEAIDDVSTQKARLDQEVKAQQQNLASMKKQKESAENALAKVGGKSVTGGFVLSKSPVAAPAPRNSDGDFGPESCSVNDPTTSGCITPRTFHMYKEVKKAKFKRFVGCHRNGGPFEHPKGRACDWSLQNSGFAPWHNQDTREYGNNLMAFLVRNADRLGILYVIWNRQIWFPATGWKSYSGPSDHTDHVHVSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQB1</accession>
    <name>U5VQB1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357945.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03565"/>
    <dbReference type="EnsemblBacteria" id="AGZ39002">
        <property type="protein sequence ID" value="AGZ39002"/>
        <property type="gene ID" value="AFR_03565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.717"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082427_0_0_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="24033" checksum="EC8B768745947E26" modified="2014-01-22" version="1">METRNTRDRILEAAAALLAEGGREAVSTRAVSTAAGVQAPTIYRIFGDKQGLLDAVASHGFAAYLEDKAGLPHSEDPVEDLRRGWDLHVGFGVENPAYYALIYGEPRPGVASPAARQAAEILAAQVRRIAEAGRLRVTEERAAHLIHSAGAGMTFELIGLTPEQRDPALSTLARESIIAAVTTDPPTHPDSVTGSAITLRAALDQLDALSPAERVLLDEWLGRIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQG8</accession>
    <name>U5VQG8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39044">
        <property type="protein sequence ID" value="AGZ39044"/>
        <property type="gene ID" value="AFR_03775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.762"/>
    <dbReference type="eggNOG" id="ENOG50334U4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126942_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665633" evidence="1">
        <location>
            <begin position="20"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="15219" checksum="E58BBFEE4969844B" modified="2014-01-22" version="1">MASAAPSAPVAAPPVTALATPVTGTGDNGSFAGTFTPTKFGTQGKDLVATGLLTGTVSDSSGATVGTVTRTVSMPVTIAAVTCEVLNLVLGPLHLDLLGLVIDLNRVNLVITAVQAPGNLLGNLLCAITHLLDGTPTAGGLAALLNAILAVLGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQL2</accession>
    <name>U5VQL2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2470 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358054.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04100"/>
    <dbReference type="EnsemblBacteria" id="AGZ39109">
        <property type="protein sequence ID" value="AGZ39109"/>
        <property type="gene ID" value="AFR_04100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.828"/>
    <dbReference type="eggNOG" id="ENOG5032Z9W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174720_0_0_11"/>
    <dbReference type="OrthoDB" id="1919863at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019595">
        <property type="entry name" value="DUF2470"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037119">
        <property type="entry name" value="Haem_oxidase_HugZ-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10615">
        <property type="entry name" value="DUF2470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2470" evidence="1">
        <location>
            <begin position="9"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="10520" checksum="22D57352D7D0EC96" modified="2014-01-22" version="1">MTFTPDVVAQIARHMNDDHADDNVLIVRGLGGIDTASAARMSGLDADGMDFEATVNGIAVPVRIPFSEHLTERRQVRTEAVRMYQQACAALGVEPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQP7</accession>
    <name>U5VQP7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3152 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_04140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04140"/>
    <dbReference type="EnsemblBacteria" id="AGZ39117">
        <property type="protein sequence ID" value="AGZ39117"/>
        <property type="gene ID" value="AFR_04140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.836"/>
    <dbReference type="eggNOG" id="COG5479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037318_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022603">
        <property type="entry name" value="DUF3152"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11350">
        <property type="entry name" value="DUF3152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3152" evidence="3">
        <location>
            <begin position="207"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="393" mass="40673" checksum="8B8EBE403C4A9F78" modified="2014-01-22" version="1">MNVDSAIAAAAGSADTQAAFGRPAAAETAELAEIVEAANHPGSRSGGVATAGNRPADSDSATLQLPVVPRIPSAEPPPAAGPPMIIPPPLVMPPDADAVIEGEASDTVVLAPPATRMYQKRRRAAVLVFLILATAVLVVGQIMRNEGSDQSSRVVPAASVLPPLVGPATGGNLEPTSAASTAPARRTAPAVPPDAADKAPTRSTLAASSGGFVFAGGYGPVLGSTGTLRRFKVAVEKTLGQGNGGKFADEVDRVLGDPRSWIAGRQFRLQRVPQSAASEFTIYLASAKTSERMCSDGGLKTKGFTSCRLPGQVVINQDRWQDAVPDYDAPLEVYRAYAINHEVGHQLGHGHEACAGKGEPAPVMMQQTYGLKGCVANSWPYLDGKRYAGNPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR85</accession>
    <name>U5VR85_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358847.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05165"/>
    <dbReference type="EnsemblBacteria" id="AGZ39322">
        <property type="protein sequence ID" value="AGZ39322"/>
        <property type="gene ID" value="AFR_05165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1045"/>
    <dbReference type="eggNOG" id="COG1922">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063203_0_1_11"/>
    <dbReference type="OrthoDB" id="1759731at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06533">
        <property type="entry name" value="Glyco_transf_WecG_TagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004629">
        <property type="entry name" value="WecG_TagA_CpsF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34136">
        <property type="entry name" value="PTHR34136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03808">
        <property type="entry name" value="Glyco_tran_WecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00696">
        <property type="entry name" value="wecG_tagA_cpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="28036" checksum="A25D3835BE060AD1" modified="2014-01-22" version="1">MTVEAFDRVQLDGTGIDRITEDEVVSVVREAIAEGRGGRILTPNIDILRQAQTDHGVRAYLDDADLVVADGMPLVWASKLSGTPLPERVAGSSLIWSLSRGLGYDQRSIFVIGGAPGADGVKDGATRAADRLAAKCTGLRIAGTLCPDFGFEKDQRNYADFCAKVAGAEPDLVFVGLGFPKQEAVITRLREALPAAWFIGCGAAVNFVAGDIDRAPRWMQKTGLEWAHRLGTEPGRLATRYLKHDAPYAMRLLAGAATRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRG3</accession>
    <name>U5VRG3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358938.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05590"/>
    <dbReference type="EnsemblBacteria" id="AGZ39407">
        <property type="protein sequence ID" value="AGZ39407"/>
        <property type="gene ID" value="AFR_05590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1134"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17260" checksum="2981C8197A4B1CC5" modified="2014-01-22" version="1">MIQDKLRLLSAEQQDFARLMAARLGVDAAGLAALYYLSDRGAATPTELARALKISTAATTLVVNRLVAAGHVGREPHPSDRRKVVVVPVQASVDAAYELVRSVAETVETLTAAMTAEERAAVESFLDRMTEAYQQAQAPLRDQHGSTGRPNDIGDGVLFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRU8</accession>
    <name>U5VRU8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Isopentenyl-diphosphate Delta-isomerase</fullName>
            <shortName evidence="6">IPP isomerase</shortName>
            <ecNumber evidence="3 6">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">IPP:DMAPP isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">idi</name>
        <name evidence="9" type="ORF">AFR_00880</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit. The magnesium ion binds only when substrate is bound.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="3 6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357408.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00880"/>
    <dbReference type="EnsemblBacteria" id="AGZ38465">
        <property type="protein sequence ID" value="AGZ38465"/>
        <property type="gene ID" value="AFR_00880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.175"/>
    <dbReference type="eggNOG" id="COG1443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060552_2_1_11"/>
    <dbReference type="OrthoDB" id="1345242at2"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00202">
        <property type="entry name" value="Idi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="8">
        <location>
            <begin position="34"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="active site" evidence="6 7">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" evidence="6 7">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR018427-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ38465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="21473" checksum="F4CE829BC5FCDFEC" modified="2014-01-22" version="1">MTASREAHLVELVDTLGEPVGALTVDEAHRAPGRLHRAFSVFLRDPSGRVLLQQRAAVKTRFPLRWANTCCGHPLPGEEPATAAARRLIEEVGVHGIELTETGVYSYYAEDPATGRVEYEYDHVLIGDVPADLVPLPDPDEVADLRWVTLDELSAGLENDLRSYAPWLPGVAESLITHLRAQPRPADDAPERSGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRW0</accession>
    <name>U5VRW0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359068.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39537">
        <property type="protein sequence ID" value="AGZ39537"/>
        <property type="gene ID" value="AFR_06240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1266"/>
    <dbReference type="eggNOG" id="ENOG5030YRH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2766528_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="69" mass="6775" checksum="8F89AB916BED37E3" modified="2014-01-22" version="1">MTSNDTPPRAAGLGIWGNLGIWVAFGAAAGVLAGVLFDNVALGVGLGSALGVAAWAVIIAPRTKARGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS54</accession>
    <name>U5VS54_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxylesterase bioH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359349.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07645"/>
    <dbReference type="EnsemblBacteria" id="AGZ39818">
        <property type="protein sequence ID" value="AGZ39818"/>
        <property type="gene ID" value="AFR_07645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1559"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_30_0_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28588" checksum="F06058D37DAEA987" modified="2014-01-22" version="1">MSTMQRNAVTVTGQASGQPMMFAHGYGCDQNMWRFVAPEFLDTHRVVLFDHVGSGRSDLAAYDEDRYSTLDGYATDVLEIAREFDLHDIVFVGHSVSAMIGILAAIREPGRFAKLVLIGPSPRYIDDGDYVGGFSKSDLDGMLDSLDSNYLGWSSAMAPVIMGNPERPELGAELKNSFCATDPEIARRFARVTFLADNRDDLPKLRTPSLILQCTDDVIAPAVVGDYVHEHLAGSTLVAMNATGHCPNLSAPAETVAAIRAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSD6</accession>
    <name>U5VSD6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monooxygenase FAD-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359258.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07190"/>
    <dbReference type="EnsemblBacteria" id="AGZ39727">
        <property type="protein sequence ID" value="AGZ39727"/>
        <property type="gene ID" value="AFR_07190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1467"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_19_5_11"/>
    <dbReference type="OrthoDB" id="504558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="7"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="402" mass="42746" checksum="A425B367A973EEDB" modified="2014-01-22" version="1">MARVRTAVVIGGGIAGPVVAVALRRAGIAATVYEAYAKNADGIGGELMLAPNGMAALAAIGIAEEVGAAGVPTPRMVMETGAGKRLGQFEDLPGLPTSRTFKRARLYRALVDAAVDAGVPIEYGRRLVGFEDGVDSVVAVFEDGTRVEADVLIGADGIWSTVRGLLDPGAPQPRFSGLVGLGGWARDAGLPSTAGAMHLAYGRKAFFGYRVGDDAEAGWFANVHSAEMLTAAQARAIPESEWLARLRELFADDELPALRLLDQVDPGELVNVGGLQDMPPVRTWSRGRVVLVGDAAHATTPSSGQGASLSIESAVELARCLRDLPVAEAFRAYEGLRRARVEKVIARTARINNDKAPGPVGRVVRDLVFPLVMRTFYQPERMMGWLHRYTIDWDAVVPAPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSE5</accession>
    <name>U5VSE5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family 2 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01285"/>
    <dbReference type="EnsemblBacteria" id="AGZ38546">
        <property type="protein sequence ID" value="AGZ38546"/>
        <property type="gene ID" value="AFR_01285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.257"/>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_9_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="256" mass="27579" checksum="6957B9938B4A7497" modified="2014-01-22" version="1">MMAAFSGSGGGPELEILIPAYNEERRLSETIFALARHLRWRGIDGSLRVIDNGSSDRTAECVDRAAAAGVPVTVTGCSRRGKGAAVARGMLTSRARWVGFCDADLATPAEAIDDALHHLRAGSEVVIGSRRCEGAELQLRQPLVRRVGGAGFRMLARRYSGPVADTQCGFKFFAAPAARAVFSQVRLSGFAFDLEVVARSRALGLAVTEIPVAWRDQEGSTFRLVSDGRKVAGELWRLHREVPAVGRFRRSAAGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSN4</accession>
    <name>U5VSN4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357668.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02180"/>
    <dbReference type="EnsemblBacteria" id="AGZ38725">
        <property type="protein sequence ID" value="AGZ38725"/>
        <property type="gene ID" value="AFR_02180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.441"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="18"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="100"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="19412" checksum="B8381E194D3DBD46" modified="2014-01-22" version="1">MNADAEDDFLTFVRLSTPSLMRVAFALTGQQQAAEDLVQTALERVATRWTRLTEPYAYARRIMYHEHISWWRRWRRQEVPVPQPEDRAAPGDLAGDVALRDDLRTALDQLGPRQRAVLVLRYLEDRSVDEVAEVLGCSAGTVRSQTSRALLRLREFTTEQVELATEGGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSR8</accession>
    <name>U5VSR8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359440.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08100"/>
    <dbReference type="EnsemblBacteria" id="AGZ39909">
        <property type="protein sequence ID" value="AGZ39909"/>
        <property type="gene ID" value="AFR_08100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1653"/>
    <dbReference type="eggNOG" id="COG2329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127577_1_1_11"/>
    <dbReference type="OrthoDB" id="1909149at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="3"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="113" mass="12200" checksum="AC80B90210C3DCAC" modified="2014-01-22" version="1">MLVLNRFVVPPDTQDSFTGRAHAALAALAERPGYLSGRLTRALDDPAYWTLVTEWESVGAYRRALGGFDVKVHATPLLSESLDEPSAFETLASASPGGEVVVEASDRAAEPWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT00</accession>
    <name>U5VT00_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Bifunctional histidinal dehydrogenase/ histidinol dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.23</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisD</name>
        <name evidence="1" type="ORF">AFR_08265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08265"/>
    <dbReference type="EnsemblBacteria" id="AGZ39942">
        <property type="protein sequence ID" value="AGZ39942"/>
        <property type="gene ID" value="AFR_08265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1684"/>
    <dbReference type="eggNOG" id="COG0141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3075770_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="52" mass="5604" checksum="3D0E8B386C49E82E" modified="2014-01-22" version="1">MGVLDFMKRSGFAYVTDEGAAAIAPVAVTLAEYEDFPAHAAAAQYVLDRINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT83</accession>
    <name>U5VT83_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09470"/>
    <dbReference type="EnsemblBacteria" id="AGZ40183">
        <property type="protein sequence ID" value="AGZ40183"/>
        <property type="gene ID" value="AFR_09470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1931"/>
    <dbReference type="eggNOG" id="COG0322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022933_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="2">
        <location>
            <begin position="226"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="593" mass="64134" checksum="49B13462AAF9CF6A" modified="2014-01-22" version="1">MQGTLDTLLSADGSVDPAALSLQDTTFVVLDLETTGGAPDGGGITEIGAVKVRAGEELGVLGTLVNPGERIPPFITVLTGITEAMLAPAPPIEAVLPSLLEFLRGAVLVAHNAPYDVGFLKAACARHGHPWPQVRVLDTAALARRALTRDEVPNRKLGTLAQFFHAAVQPTHRALDDARATVDVLHGLIERLGSFKVHTLGDAIEFAKAVTPTQRRKRHLADGLPHAPGVYVFRAADERPLYVGTSVDIATRVRSYFTASEKRARMSEMIGAAARVEAVECAHSLEAEVRELRLIAAHAPPYNRRSKYPERVVWLKLTAEAFPRLSVVRQLADDGAAYLGPFSSRRTAELAAAGVYDAVPLRQCTHKLSTRTITPACALAELGRCPAPCEHKITPEEYGARAAIPFRTATYGDPEPLVEALMARIDVLSERRRYEEAAVIRSRLVALLRATVRMQRLGALTSIAELVAARRNDSYGWEIIVVRHGRLAAAATSPPKLHPRPTLDMARATAETVLPGPGPVPSASAEETERILAWLERPDTRLVETSGDWVSPARGAARFTRLLNRAEAGGSRRDSSVRSSVTARTDDGRSLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTE9</accession>
    <name>U5VTE9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative subtilase-family protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_09895</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359980.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09895"/>
    <dbReference type="MEROPS" id="S08.069"/>
    <dbReference type="EnsemblBacteria" id="AGZ40268">
        <property type="protein sequence ID" value="AGZ40268"/>
        <property type="gene ID" value="AFR_09895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2018"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007528_0_0_11"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665738" evidence="6">
        <location>
            <begin position="31"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="7">
        <location>
            <begin position="222"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1098" mass="114574" checksum="5CC0F905A7AA4C18" modified="2014-01-22" version="1">MRRRKLPAFLLGVVTITASATLAGAPAAQALPAGAPADKPAPSTTSSVTLITGDRVTVRGDGTTSVEPGPGRRDMRFATYRTATGRHVVPVDALRLLRAGKLDARLFDLTALTAAGYTDERTRELPLLVAYPKAQGGKARSATTVQGARVTRDLSAVGALAVRADRAARASLWEAMTGGTDAARTLQPGIDRIWLDGKRKINLDVSVPQIGAPTAWQAGFDGTGVTVAVLDSGIDATHPDLAGKIVAAENFTDAPDADDNVGHGTHVASTIAGTGAASNGRYKGVAPGAKLLIGKVCGTEFCEESAILAGMAWAAERAPIINISLGGDDLEGIDPLEQAINDLTARYSTLFVVSAGNAGGFAPVGSPASADAALAVGAVDRDDQVADFSSRGPRVGDNAIKPEITAPGVEIVAAKAAHDVIGGPAPVAGYSTLSGTSMAAPHVAGAAAILVQQHPDWSSQQRKTVLMGAAKPTEGFDVFAQGAGRVDVARAIKQSVGVDEGSVSFTGGQWPHGDDTPESKTVTYRNSGTAPVTLTLAVDTTGPAGLFTVSPGTLTVPAGGTATTTLTSDTSVDGPEGLLSGYLTATGADGLKVETPIAVNREPESYDLTFKALGRDGAAAENAYISLFDLKTGTQYQIFGADSAKRLPKAEYGMYSFIDSETESSLVAQPKLAITGPATITVDARDAKPVDVKVPRADAVPTFIVLGADWTIEQGVIGAGIIGLEFDQLFAGQRDPKSSFDGFLSSAVIALAQGDGVNSPYVYDLAYFREQKMLDGLRKNVKAKDLATIKATYATEATGAQGVKANSARYTPDGSYWGVFIPFDPPFRRTEYVNTDNKAQWSSAFEQQLPPVGDGWPETLASATATPATFRGGRTYQQEWNRAVFAPTVAGAEYDWDYVSRLGDLLWVGMPSFGEGPGHPGFSTIDSARTALYRGNTLVTEVDTEWAEVELPAAAADYRLEKSVTRSAPHQFSTQVSGTWTFRSGNVAGETPKQVALSTLRFSPKLDDRNSAPAGRPFVVPVHVDHQAGTGTANKVTVDVSYDDGRTWTKAPVAGSGDHRVAAVWHPRGKGFVSLRASATDQHGGTVTQTVIRAYAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTQ3</accession>
    <name>U5VTQ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative ppGpp synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_09465</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelA/SpoT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09465"/>
    <dbReference type="EnsemblBacteria" id="AGZ40182">
        <property type="protein sequence ID" value="AGZ40182"/>
        <property type="gene ID" value="AFR_09465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1930"/>
    <dbReference type="eggNOG" id="COG0317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012300_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015969">
        <property type="term" value="P:guanosine tetraphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="55"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="3">
        <location>
            <begin position="390"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="569" mass="62686" checksum="24609189EF93D469" modified="2014-01-22" version="1">MSFLSFQGNDDDPVTTLTRTHRAIHPSADAGVLRRSYAVAENMHRGQFRKSGDPYITHPLAVAQICAELGMDTTTLVAALLHDTVEDTSYTLETLNGDFGPEVTHLVDGVTKFDKAFYGKVAEAETIRKMIIAAGKDVRVLVIKLADRLHNMRTLDARSPASRARIATATLDVLVPLCDRLGIQALKRDLDDVVLYHLEPDSFSRIDEHVKNRPGWSEYVEDVRVKAAAALRRSRVDAKVAPRPRHYYSIWKDTVAGGHAVPLDLPRIVVIVDGPETDCYAALGAIHGQWRPVAGRFKDFIASPKNNLYRSLHTTVTGPEGRLVEVLIRTVSMHRCAEYGVATGYRYPKSPEASDATGADQLTWLKRVLDWEQDTTDAGQFLASLRCDLAEGQIQVFAHGNTFDLPAGSTPVDLAYEVSADKGDQTLAATINGRLAPLSSPLRDGDVVEIFTETDGHVEVEPGAAPRGPRKEWLGFVKSTQAQMQINRYFAEENAPGISIADKVRLGRATIGLTLRKHDRGLASEVPLRRLAEELGYPDLETMLVAVTERNLEPDQVVEQLIALVDHPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTS3</accession>
    <name>U5VTS3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_03735</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03735"/>
    <dbReference type="EnsemblBacteria" id="AGZ39036">
        <property type="protein sequence ID" value="AGZ39036"/>
        <property type="gene ID" value="AFR_03735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03735"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032783">
        <property type="entry name" value="AraC_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12852">
        <property type="entry name" value="Cupin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="5">
        <location>
            <begin position="212"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="328" mass="35620" checksum="4CBE1F2685971DAC" modified="2014-01-22" version="1">MMDTLTGLLDGPRARGAFLLRSILNPPWCLRIQDEAPLSLVTLVRGSAWIIPDDDEPTLLHPGDVAILRGPDAYALSDAPATPVQVVIHPGQRCTTVEGEEQRDLVDLGVRTWGGSLDGETVMLSGTYQLHSEVSRRLLDALPALLIRPAGTGDRTLVDLLEKEMTGTSTAQELVLDRILDLLLVSVLRAWLDSTDPGVPGWHQAQSDPVVGPALRMLHDDPARAWTVADLAVEAGVSRASLARRFTELVGEPPMAYLTRWRLTLAADLLRTADATIGGVARRVGYGSSFALSTAFKRERGVSPQEYRRRSGASPQDVRHRPGVVRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU66</accession>
    <name>U5VU66_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <shortName evidence="5">G6PD</shortName>
            <ecNumber evidence="5">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">zwf</name>
        <name evidence="9" type="ORF">AFR_10625</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10625"/>
    <dbReference type="EnsemblBacteria" id="AGZ40414">
        <property type="protein sequence ID" value="AGZ40414"/>
        <property type="gene ID" value="AFR_10625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2166"/>
    <dbReference type="eggNOG" id="COG0364">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013524_5_0_11"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="PTHR23429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G6PD_N" evidence="7">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="G6PD_C" evidence="8">
        <location>
            <begin position="187"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ40414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="483" mass="53863" checksum="2DA524A0A507A473" modified="2014-01-22" version="1">MIFGVTGDLSRKKLIPAVYDLANRGLLPPGFVVLGFARRDWGDGDFESLAYEAAKKGARTPWREDVWTRLASQFQFVPGSFDDDAAFDHLATTLDELRERNGIAGNAAFYFSIPPAAFPLVLNQLARTGMADNAKSGGWRRVVVEKPFGNDLQSAMSLNQLVDEVFTPEDVFRIDHYLGKETVQNILALRFANNLFEPVWNSKYVDSVQITMAEDVGIGTRAAFYDASGAARDVLQNHLLQLLALVGMEEPTSFDPNDVRAEKLKVLKAISLPRDIAKGSVRGQYLPGWVAGERAVGYLEEKGVPEDSTTETYAAVRLGIQNRRWAEVPFFVRVGKRMPRRVTEIAIMFKKAPHLPFDPADIEMLGNNQLVIRVQPDEGVVLKFGSKVPGTTMEVRDIAMDFQYGEAFTESSPEAYERLVLDVLVGDRTLFPDAAEIEQSWKVIDPLEAAWAGTKPEPYRSGEWGPRASDEMLAREGRAWRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU73</accession>
    <name>U5VU73_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Pyruvate kinase</fullName>
            <ecNumber evidence="5 14">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">AFR_10365</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="5 14"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360164.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10365"/>
    <dbReference type="EnsemblBacteria" id="AGZ40362">
        <property type="protein sequence ID" value="AGZ40362"/>
        <property type="gene ID" value="AFR_10365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2112"/>
    <dbReference type="eggNOG" id="COG0469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_2_11"/>
    <dbReference type="OrthoDB" id="1098983at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PTHR11817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 14 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 18" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="14 18" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666091" description="Pyruvate kinase" evidence="15">
        <location>
            <begin position="19"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="PK" evidence="16">
        <location>
            <begin position="5"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="PK_C" evidence="17">
        <location>
            <begin position="356"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AGZ40362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="482" mass="51229" checksum="4033FA06C5EA3D25" modified="2014-01-22" version="1">MAVTRRAKIVCTMGPATASPERMLGLVEAGMDVARLNFSHGSHEDHQKVYDLVRAAAKETGRAVAVLADLQGPKIRLGKFANGPHLWNTGDVVTITSDDILGTPDRVSCTYKKLPQEVKVGDRLLIDDGKVAVEVSAVEGNDITCLVTEGGPVSNNKGVSLPNVAVSVPAMSDKDEADLRFALSLGVDLIALSFVRSPDDIKLVHQIMAEEGTTLPVIAKVEKPEAVTHLEAIVLAFDGVMVARGDLGVELPLDQVPLVQKRAVQLCRENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPVLTVSTMAKIVTTTESGDFGVPVLQHDPRTHSGALTVAASRIARNIGAKALVAFSQTGDTVRRLSRLHCELPLLAFTPVPEVRDQLALSWGVETFLTDFVQHTDDMFRQVDQSMLGLGLAKPGEYVVVVAGSPPNAPGSTNTLRVHQLGSLVDPATVTEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUK6</accession>
    <name>U5VUK6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PBS lyase HEAT domain-containing protein repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05605</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358941.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05605"/>
    <dbReference type="EnsemblBacteria" id="AGZ39410">
        <property type="protein sequence ID" value="AGZ39410"/>
        <property type="gene ID" value="AFR_05605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1137"/>
    <dbReference type="eggNOG" id="COG1413">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_415999_0_0_11"/>
    <dbReference type="OrthoDB" id="385053at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="659" mass="69647" checksum="656DCCF66E29EA45" modified="2014-01-22" version="1">MQDDVGGIIDGLGSADAEVAEEALDDFFDSVWHQGTVYPETVTVVPGLVELAVTEGVHNRSHLLRILGGICDPEESYGDDQPAVRAAVVAHAARLEQLLDDPDPELRDLTAYVLGQCGDRAALTGRWPLETDPQVRASLLMGLGSVDLAREAALTEPFPVPVAAALVYARAGLPLPSETIIPIAAAFGAAEPWDSPWPGQDGALSEVLELLDGASAGALTVALTSAPGGKRQPGVRSRLRAAEAMADRFESKRSAPVELMPRLRELLTDDDAEVRAAAMQAVTHAGAPSAAVADILAEMAQGTADEERWNGPAHLALNVLARLGDPRWREPLIAAWEAGQDPSVVSTLGDFPPPFDPVVLAAARRRLRVLTAQPMPQANAVIALVVLLRNWGPAAADAAEDVLAARFAAPWIVPATLAGMGERALFAVPALTAQADRGQVRDGHAVWRLTGDPGPLVRAGRQLLTDPRGRLQWELELLADAGPAAAPLVPLLTPWLTGRAAPNYEGEDQIAAARVVWRATGDTAAVLPTVDAALRTGDSPARSAAKFATELPDVTSLVPALRKAVSGDWGVVDAARALWRSGTPVDDLVRPLLTAAADTYVHTEAVDLLVEMNATSALPGLTALATQDNRVVQGSAALVWKDDRLRRQLHEAIRTLRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUM5</accession>
    <name>U5VUM5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360473.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11165"/>
    <dbReference type="EnsemblBacteria" id="AGZ40522">
        <property type="protein sequence ID" value="AGZ40522"/>
        <property type="gene ID" value="AFR_11165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2274"/>
    <dbReference type="eggNOG" id="COG3021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060500_5_0_11"/>
    <dbReference type="OrthoDB" id="1303819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666097" evidence="1">
        <location>
            <begin position="29"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="63"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="31397" checksum="3F80C58B83EC8CC9" modified="2014-01-22" version="1">MRSPEPARRSLMPIAALAATVILLVTGAASVNEPATNIAAGGEAVPESGRAVRPAPAPTVRVMSFNTCGAACNDGEVPRTAAYLARTVIAQRSEVVFLQELCRSQFRKIQSLTRTRGYQAIFAAQTRSRRCGGDFGVALLVRGKTWGKVVRRLPGRPGFERRVLLGAQAQVAGRQTFVAVVHTSPSVRAGRPRQLRAVADFLRPYASGPAIVGGDFNTLPGDPGLSGLLSRTAGGTGRYLELDQLRRRPTFETVSRKIDYIFASESSFASPAAAGLRTTMSDHRIYVGTFRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUN1</accession>
    <name>U5VUN1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase M75, Imelysin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_05755</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EfeM/EfeO family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358971.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05755"/>
    <dbReference type="EnsemblBacteria" id="AGZ39440">
        <property type="protein sequence ID" value="AGZ39440"/>
        <property type="gene ID" value="AFR_05755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1168"/>
    <dbReference type="eggNOG" id="COG2822">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050342_2_1_11"/>
    <dbReference type="OrthoDB" id="1430947at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14656">
        <property type="entry name" value="Imelysin-like_EfeO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028096">
        <property type="entry name" value="EfeO_Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018976">
        <property type="entry name" value="Imelysin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034981">
        <property type="entry name" value="Imelysin-like_EfeO/Algp7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038352">
        <property type="entry name" value="Imelysin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13473">
        <property type="entry name" value="Cupredoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09375">
        <property type="entry name" value="Peptidase_M75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665359" evidence="3">
        <location>
            <begin position="21"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Cupredoxin_1" evidence="5">
        <location>
            <begin position="35"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M75" evidence="4">
        <location>
            <begin position="143"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="40165" checksum="1E0C690CFE359D6A" modified="2014-01-22" version="1">MRTTRILALAAVGVLGGALAACGGDSADDTAAAGSAGGPITVKASDTTCEVAKNAVDAGTSVFTITNGGNKVTEFYVYAPGDRVMGEVENIAPGLSRELHVELPSGTYETACKPGMIGKGIRAALTVSGSAAPLTDDAKLADATASYSRYVKSQTGALLPKTEEFVAAVKAGDVDKAKALFPIARTYWERIEPVAEIFGDLDPKIDGREEVIEEGMEFTGFHRLEKDLWAAKPDVSKDGPIADQLLTDVKEIVAKANAEKLSPLQLANGAKGLLDEVATGKITGEEDRYSHTDLWDFNANVEGSKAAIASLRPVLEERDPALVKTLDTEFANVDTTLTKYREGDGWKLHNKLTEADLKGLSDAINALAEPISKVAAVVSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUR0</accession>
    <name>U5VUR0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358917.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05485"/>
    <dbReference type="EnsemblBacteria" id="AGZ39386">
        <property type="protein sequence ID" value="AGZ39386"/>
        <property type="gene ID" value="AFR_05485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1113"/>
    <dbReference type="eggNOG" id="COG2253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084427_1_1_11"/>
    <dbReference type="OrthoDB" id="1605258at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014942">
        <property type="entry name" value="AbiEii"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08843">
        <property type="entry name" value="AbiEii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23506" checksum="D1A9A68A9C82A717" modified="2014-01-22" version="1">MDPRHRRLAEIALSAAGSDYGLALAGGYAVREHGMGDRPSGDVDLFTDWQRRADFPAVADLVIKALTENGFLVDVDARADTFARLLITANDEPGTEPQKMELAADWRAHPPVTLNIGPVLHPDDAVGNKMAALYGRALARDFLDVDAVLASGRYSSEQLLQLVEAADAGFDRALFADTLGALGQISDTAFAAYGVNAQAVQRLRQRFADWRNELTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUW5</accession>
    <name>U5VUW5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-binding region ATPase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12485</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360842.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12485"/>
    <dbReference type="EnsemblBacteria" id="AGZ40783">
        <property type="protein sequence ID" value="AGZ40783"/>
        <property type="gene ID" value="AFR_12485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2536"/>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002554_2_1_11"/>
    <dbReference type="OrthoDB" id="1954053at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013587">
        <property type="entry name" value="Nitrate/nitrite_sensing"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08376">
        <property type="entry name" value="NIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665785" evidence="3">
        <location>
            <begin position="22"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="4">
        <location>
            <begin position="409"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="545" mass="57244" checksum="48683816A8BB72E5" modified="2014-01-22" version="1">MKARLGVALLVVLWAAATVPAVTTAVRIVSERAAADRMTPLIGRAVLALEAERRLSVAARPGQSAPPAGFADQRSRTDQAGETLRGAADGWASRTRATDETRAADALVRRMTGLPALRAAVDDGQMPRRTALDAYTAIIGSPLGDSALTRTRELLSEEDALLAATAGVARVTDADRLRLAELAGARRTLLVTAAVTPPAADRLRSMEDALILKPGPVPDGWSAAFNTANTALWDGRTDAAREARDETTPSAVAAIAWACLVGAVGLIAVVAVLMYPRRSTRRVPAPATVPAAAGEQVAAPQAGPGLDALLRDLERRNQSLVHRLLRLLDGLARHESDDETLGELFRADHFANRIRRNLEKAIMLAGDLPGRRWNRPVPLADVVRAAASEVPEFERVSTSRIAPVHLTGAAVTGTMHLLAELIENATTFAPAQTRVRVTGEPAPVGYEVTVADVGPGMTDDDLATAYAVLSDPSPRPGGTWWGLYATGRFAERLGIEVRLRNAPGGGLEASVIIPAALLVDAEAAEAVPDDATGEMPAIAGGVGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVI4</accession>
    <name>U5VVI4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361072.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41013">
        <property type="protein sequence ID" value="AGZ41013"/>
        <property type="gene ID" value="AFR_13635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2769"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_0_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="36"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="32991" checksum="6EC83DFD2E76ABC9" modified="2014-01-22" version="1">MDHRAETRDFLTTRRARITPAQAGLPSYGTSRRVPGLRREEAAMLAGVSVDYYTRLERGNLSGVSDNVLEALVRALHLDEAERDHLYDLARQANAGPRARATRRRTTASLRPAVQQLLDAMTSAPSYVRNGRLDILGANNLARAVFAPVFRTTAGRPNLARFIFLDPASQDFYRDWEQLAEDVVALLRGEAGRNPYDKDLTDLIGELSTRNDRFPTWWAAHDVRLHRSGVKRLHHPLVGDLTLSYESMDLTADQGLRLNAYSAQPASPDQDALNLIASWAASPTSPISPTQSQEHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVM1</accession>
    <name>U5VVM1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07355"/>
    <dbReference type="EnsemblBacteria" id="AGZ39760">
        <property type="protein sequence ID" value="AGZ39760"/>
        <property type="gene ID" value="AFR_07355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07355"/>
    <dbReference type="HOGENOM" id="CLU_2566144_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="81" mass="8937" checksum="2903AAFE40DA590E" modified="2014-01-22" version="1">MPHVDGQVDQAVPDLRITVMAEVPIRLILDSPGINGDWPGRVAGRRFPVGVTVRMKVHGRGHSAQHAGRDPLEYFSRSVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWE5</accession>
    <name>U5VWE5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40035">
        <property type="protein sequence ID" value="AGZ40035"/>
        <property type="gene ID" value="AFR_08730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1776"/>
    <dbReference type="eggNOG" id="ENOG5031HUI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1276861_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666146" evidence="2">
        <location>
            <begin position="20"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="21935" checksum="A69CBEDE55814781" modified="2014-01-22" version="1">MAAGLALSVGLAPAVAAQAAPAAPKAPAKPTAVQIAGKFPGDKIVVQQAERADLFKRLLSEVNWLANTSPTTSRPQADKMGPKFVVTVLIKDKANQVYDLYPNAAGGPRAYRPAKQPTGKKAAGWFYGRLTMSESLRLSGVPLKAKPDVVQGGIGGGVGEDVAVDEVDPVKNVNGVISDFRRLFLLNTAVLVVILLGLGGIAFLIRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWT8</accession>
    <name>U5VWT8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14875"/>
    <dbReference type="EnsemblBacteria" id="AGZ41257">
        <property type="protein sequence ID" value="AGZ41257"/>
        <property type="gene ID" value="AFR_14875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3021"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066193_0_0_11"/>
    <dbReference type="OrthoDB" id="1757088at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="184"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="29877" checksum="D2F731961E41267A" modified="2014-01-22" version="1">MRSAAPVRLAAPAWDIAVPVRPSRLAGVTMAGFSDRATSLVDVEVVPHPGVMVVFDLGDRALVVADGKGREQRGCVVAGLAPDRARGQGPAGSFSCLQVRLSPVVARAVLGAAPELSGVVLSLDDLWGADAVRIQERLRAARSWEERFTLVDVALARRHDEGRAVDPEVAFCWGQMMASRGRTRVDGLAAEVGWSRKRLWSRFRSQIGVAPKRAAQLIRFDAAAHRLAAGQAAAVAAVDSGYADQSHLHRDVLTFTGVTPATVASAPFLAVDDVAWPDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWY2</accession>
    <name>U5VWY2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MDMPI_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361578.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16105"/>
    <dbReference type="EnsemblBacteria" id="AGZ41503">
        <property type="protein sequence ID" value="AGZ41503"/>
        <property type="gene ID" value="AFR_16105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3268"/>
    <dbReference type="eggNOG" id="COG1576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051661_1_1_11"/>
    <dbReference type="OrthoDB" id="1805421at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017517">
        <property type="entry name" value="CHP03083_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017520">
        <property type="entry name" value="CHP03086"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024344">
        <property type="entry name" value="MDMPI_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11716">
        <property type="entry name" value="MDMPI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03083">
        <property type="entry name" value="TIGR03083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03086">
        <property type="entry name" value="TIGR03086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MDMPI_N" evidence="1">
        <location>
            <begin position="18"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="20620" checksum="D9ACEA91DCA45537" modified="2014-01-22" version="1">MTLSDRPDDRHRRIAGDFTDRVEGTGSWDVPSPVAGWTARDVVRHLTDWFPGFLAAGSGIELPRGPSVDEDPVAAWRVHSDGVQAVLDDPGSAQRELTNPHLGSVPVPAAIDRFYTADVFMHTWDLSRATGQDDSLDAAFCAQLLAGMESMEDVIRSSGQYGARVEVPADADVQTRLLGFIGRDPYWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX14</accession>
    <name>U5VX14_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361391.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15250"/>
    <dbReference type="EnsemblBacteria" id="AGZ41332">
        <property type="protein sequence ID" value="AGZ41332"/>
        <property type="gene ID" value="AFR_15250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3096"/>
    <dbReference type="eggNOG" id="COG5563">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_675493_0_0_11"/>
    <dbReference type="OrthoDB" id="1712871at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="407" mass="42108" checksum="16CC8B0C0DD0BCFF" modified="2014-01-22" version="1">MISENDLRDRLDAVDVPPTGLRVETLVAQGRKRVLQRRTVRAACGVALATGLLVGVPSVLLNPGGGGTTNPIGDEPASSASAEGPREPTAVPGLPTVRCAGKMLAVPAGMTTVQPVAVDTSGRYIVGNDVRMSELDTPEGKVAGVGDSQAILWTDGTPQALPKLGDWVSASAVNADGVVAAIAGPKDKWADAVIRYTGGVPHKLTPLTGKWTFESAEINTAGDIVANATRQSTAGNRIDAVMLWKAGAQTATKLPLPLGAEARSILDDDRIVGVLAKGSQISDLNSYVWTPQGKGTKLENPQGQNGSVYEARGDWATGNLWPAGAVARWNLKTGEVTEVAVDAPGNSINASGWIASAGTIMRDDANVELQLADDDVKGEPIAVSDTGVVVGLPFDGSSGAISWRCER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXJ5</accession>
    <name>U5VXJ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361592.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41517">
        <property type="protein sequence ID" value="AGZ41517"/>
        <property type="gene ID" value="AFR_16175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3282"/>
    <dbReference type="eggNOG" id="ENOG5033F6Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200928_0_0_11"/>
    <dbReference type="OrthoDB" id="2067364at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="73" mass="7941" checksum="22C5FA904D19CED0" modified="2014-01-22" version="1">MAGTEHEVNRSARSGRFVKETTAQRDPKSTTTEHVGGDKSGGDQEVNRSTSTGRFVKETTAELHPATTETQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXU1</accession>
    <name>U5VXU1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LuxR family two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_17650</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362089.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17650"/>
    <dbReference type="EnsemblBacteria" id="AGZ41808">
        <property type="protein sequence ID" value="AGZ41808"/>
        <property type="gene ID" value="AFR_17650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3578"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="134"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="21441" checksum="16E69CB4437D0102" modified="2014-01-22" version="1">MIRVLLADDEELIRVAVAALLGLEPDLEVVAQAADGRTAITQALAHRPDVAVLDLQMPALDGLEATAELRRVLPGCAVVILTGRGRPAQIPRALAAGAKGFLPKGSPGGALADVIRRVHGGGRYVDPALAAEALSLPACPLTERELEVLRHAEPDVPVSVVARRLRLSPGTVRNHLTAITTKLGVPSRAAAATVARENGWLGSAPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY69</accession>
    <name>U5VY69_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Anti-sigma regulatory factor (Ser/Thr protein kinase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361928.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17245"/>
    <dbReference type="EnsemblBacteria" id="AGZ41727">
        <property type="protein sequence ID" value="AGZ41727"/>
        <property type="gene ID" value="AFR_17245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3497"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072253_0_0_11"/>
    <dbReference type="OrthoDB" id="1832160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025847">
        <property type="entry name" value="MEDS_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14417">
        <property type="entry name" value="MEDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MEDS" evidence="2">
        <location>
            <begin position="10"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="1">
        <location>
            <begin position="197"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="33662" checksum="EEC90D072BCCA28B" modified="2014-01-22" version="1">MRDEASGCLTHDAMFYRDDHEFAATLVPYVRAGLAEGQAVAAAVTGPNITLLRDALGTDHTQVSFFDRDDWYARPATTVARWHRVLTEATRRGRRHVRLVGEVGFGGHLPSWHRYEAALNDIFAAAPVSIVCPYDTRVLPPELLDEARRTHPTVVAGEGRQPSDAYVTTAQFLLNVPEALPPVSGPPVLTMELVGGVVQARQAVTALVEANGWTGLDRADDLVLALSEITTNSLRHGGGRRELRVWVDGCTLTCEVADDGPGPADPLAGYRPPDPAGTGGRGLWISRQLCDALGIGHRDGATQVRFAVLLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY81</accession>
    <name>U5VY81_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362171.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17855"/>
    <dbReference type="EnsemblBacteria" id="AGZ41849">
        <property type="protein sequence ID" value="AGZ41849"/>
        <property type="gene ID" value="AFR_17855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3622"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_4_1_11"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="10"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="152"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="31118" checksum="6657B1BD3024F3EB" modified="2014-01-22" version="1">MPPAKTATLPLLAAAATMILWASAFIVIRSAGAHYSPGAMALGRLAVGATALTLVASFRPRRLPRGRPLLLVIVYGVLWFGIYAVLVNAAEHHLDAGTTALVINVGPILIAVLAGLYLKEGFPRGLMIGLVVAFAGVATIAAATSTGRHDTTGVLLALGAAALYAVGVLLQKQALDFVDPFTATWLGCVAGAVACLPFAPALVDEVRAAPGSATLGVVYLGVFPTAIAFATWAHALKRMSAGTLSSSSYLVPGLAVLMSWLLLDEAPAPLALVGGALCLTGVALTSLLQISGNKSPEPLDSPRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYH7</accession>
    <name>U5VYH7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362317.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18330"/>
    <dbReference type="EnsemblBacteria" id="AGZ41944">
        <property type="protein sequence ID" value="AGZ41944"/>
        <property type="gene ID" value="AFR_18330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3720"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="26005" checksum="251F94C0582297E0" modified="2014-01-22" version="1">MASEIELVDVQAGYGRAAPVLRGFSVAVPAGSVVCLVGPNGAGKSTVLKVASGLLKPRSGRILVGGEDVTGLGPQKMLAAGVALVLQGHSVFREMTVAENVLLGAYTLKDKSKLAARTAFVKELFPVVAERWNALAGLLSGGQQKQVEFARSLMVDPKVVLLDEPSMGLDPKATSIIFDQVVRMRDAGTAVLLVEQNARRALETADTGCVLDLGRVHISGPAAELLADPQLGELYLGGRPAEPPVAPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYL3</accession>
    <name>U5VYL3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360810.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12325"/>
    <dbReference type="EnsemblBacteria" id="AGZ40751">
        <property type="protein sequence ID" value="AGZ40751"/>
        <property type="gene ID" value="AFR_12325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2502"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_4_1_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="847"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="914" mass="97683" checksum="0E4B8B4A6232366B" modified="2014-01-22" version="1">MDLDGYGLVGRADKLADLGDFLQEAARTGSAMLLAGEPGIGKTALLNAAAERAAAMGMRVIRGSGAEHETALCFSGLHQLVGPLSAELAQLPGPSRYALEVALGFGAGSTPAEMTVLHGVLSLFAEAARKRPLLLVVDDLHVLDRPSRSVISFVARRAVGRRFGVLAAVRSETMGSYDSGLTEVLVPPLHEADALHLLSERFAELPRRAALDVVRQAQGNPLALLEFGALAGMSGAPRDAVPGTGPAKEVRALYAARVARLPAGTRELLLLAALESSGDLGVLHAAAGEGGLDAFGAAERDLLIQVSANGRHVHFQHPLVRSAVIEGATLEQRRSAHQRLADVLVDRPERHAEHLSMAAAGPDEPVAHLVEQWARQSLQRGDVLGAMDRLRRAAALSPDRSVGDRRLAYAAYIGVSVAGHLELSCQLMRELDGAAAMSRSLPVAAARGYLHLMTDGDAATAYRILVEAISPLAVTGATDLDELMPALYTLLLICHYSGRADLWAPLRDLVASLPRDSASSPVSMVHILEDVGAVPDELLRDLDGQIARLPEVTDADVIIRTALTASYLDRLSGCHQSLTEMIRDARDSGTIGGALPALLMCAMDDLYSGRWVEAQGMATEILVLGRETGFHLPSQFGRYVMALLAAHRGDTDTCAALAQELHEWAASRRLGRLTRWAHMAQTRAALGSSDFETAFRHAAAITAPGWLPPFNIEALWTALDLTESALRSGRADAAREHVAAMRNSGVARISPRFALMTATMTALTAPPAEMAHHFDRALALPGIEHWPFELARTELAYGEQLRRQALKAEARVQLSAARDRFERLGATPWVARASAELRATGNARASKARVGVTLTPQEWEVTELAAAGLANKEIAARLMLSPRTVSSHLYRVFPKLGISSRAALRDALNGRRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYY9</accession>
    <name>U5VYY9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360950.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13025"/>
    <dbReference type="EnsemblBacteria" id="AGZ40891">
        <property type="protein sequence ID" value="AGZ40891"/>
        <property type="gene ID" value="AFR_13025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2645"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_8_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="15669" checksum="479D6454D1B5DCC0" modified="2014-01-22" version="1">MTASELTAAVTATYDIWMRTVDLTGPALARHRLTSATFQALWVIDPDEPPPSMKVVAERLHCNASNLTFMANQLIDRGLVERVTDPADRRFRGLHLTAKGRQVRAEVVQAALAGNPLARLDEGDLRQLVTLLNRALDSPLGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZB2</accession>
    <name>U5VZB2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361099.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13770"/>
    <dbReference type="EnsemblBacteria" id="AGZ41040">
        <property type="protein sequence ID" value="AGZ41040"/>
        <property type="gene ID" value="AFR_13770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2796"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080242_0_0_11"/>
    <dbReference type="OrthoDB" id="1432684at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="256" mass="26401" checksum="A89418024A2B6B93" modified="2014-01-22" version="1">MSTTYGTAIKPEEHTATEPAPEPAPPAPRHRLRRTLSVLRTVTVVLILLAAAVGGGYYVVTHRLADRAYVNAGTAVLTAEPITVGSADAAVVTRMLTAERRSVTAGAALATITVTADGRGNKVQELRAPAAGIVTEVNVAPGQIARAGEPIVTLYDPAKLVFKVDVPLETLRKLRLGMTAYVTGPGLSGRIATTLQEVQPRVTTGEAAATGDGLTVVLVPDTAVLSTVRTLVPGLQFAVVVDTTTAVGQTPAVNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZI6</accession>
    <name>U5VZI6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NlpC/P60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_20160</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362676.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20160"/>
    <dbReference type="MEROPS" id="C40.007"/>
    <dbReference type="EnsemblBacteria" id="AGZ42304">
        <property type="protein sequence ID" value="AGZ42304"/>
        <property type="gene ID" value="AFR_20160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4089"/>
    <dbReference type="eggNOG" id="COG0791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034085_1_0_11"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665388" description="NlpC/P60 domain-containing protein" evidence="2">
        <location>
            <begin position="34"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="NlpC/P60" evidence="3">
        <location>
            <begin position="213"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="329" mass="35145" checksum="4172DD89E575C2E3" modified="2014-01-22" version="1">MKRIEARSAAILRGIACAVAAVGILLAPGVAHATPSPSNIEKQIDEQWNKLEPVIEQYNDVHNQLIKNRAQLKKIDKRLAPLELQVDLAMSQVGNMAGQAYMQGSPNAVKAMIVSGSPTGLTEKLTFLDQLSRHQRESIAGVAKLRDQLAGDKRDLKTLTDAVAARDKLLGQQKKDIQKKVDDLQKLRIRAYGASGSSGGAFRTGPCPVDYDNSKGGRAAQKACSLIGKPYIFGAEGPSGYDCSGLTKVAWAAVGVHLEHFTGDQIGAGRAVSRSELQPGDLIFYGSPVHHVGLYVGGNTMVHAPHTGDHVRMASIDRSGAITGMRRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZP6</accession>
    <name>U5VZP6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GRAM_POS_ANCHORING domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_19850</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362614.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19850"/>
    <dbReference type="EnsemblBacteria" id="AGZ42242">
        <property type="protein sequence ID" value="AGZ42242"/>
        <property type="gene ID" value="AFR_19850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4028"/>
    <dbReference type="eggNOG" id="ENOG5030RC6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_768656_0_0_11"/>
    <dbReference type="OrthoDB" id="2131707at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019931">
        <property type="entry name" value="LPXTG_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50847">
        <property type="entry name" value="GRAM_POS_ANCHORING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0572">Peptidoglycan-anchor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665913" description="GRAM_POS_ANCHORING domain-containing protein" evidence="6">
        <location>
            <begin position="36"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="GRAM_POS_ANCHORING" evidence="7">
        <location>
            <begin position="323"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="293"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="360" mass="36345" checksum="64B8C438582A0590" modified="2014-01-22" version="1">MTLRWRTGRTFALLAAGAALAAPAAVGLTATPAAAAPGPIDMNARLTGPATVVRGELALFTIDSVIPGPPAPTNVKLILTLPDGFSYAQMGGGSGPCTADAARRVVTCIPDPGEPSGLPGARQWEVYARASSSLAAGTELTTALEVTADGDETNPADNRDTVTTRVTGPAATSIRATGPKGPIVPGSKFRVTVQVHYADGPPIDDFAMFAHFGGDWFLGAELVGVPAQCSGDPGSVMCEIDRRVEPNTDFDLVWEFGSTGDPAGYHAPTEQLRFQTFAFGDPVQTSIELTFATATPGTPSPTPTGTGTGGGTGNGDGQAGGGLPITGTATGSLAATGVLLVAAGAGAVLLGRRRRRSSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W045</accession>
    <name>U5W045_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_20650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20650"/>
    <dbReference type="EnsemblBacteria" id="AGZ42402">
        <property type="protein sequence ID" value="AGZ42402"/>
        <property type="gene ID" value="AFR_20650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4187"/>
    <dbReference type="eggNOG" id="COG0531">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="450"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="497" mass="51597" checksum="571CDC3E52DF3C46" modified="2014-01-22" version="1">MPTSTAPPRPSQLSTALARDRLGVAAVVFFVMSAAAPLTVVAGVVPTGLAVTGLTAISIAFLAVAIVLAVFAVGYVAMARHIANAGAFYAYIARGIGRPFGVGASWVALLAYNSFQVASYGGFGAIAAPLFADWFGVEIQWWIIALAAWALVAVLGVRDVAVNGKVLATLLVAEIVLVVVFSIADLMAPDFAASSAPVDPSSLVGAGAGALLVMAVTGFVGFEQSVVFSEESRDPRRTVPRATYIAVALIAVLYAFSSWAMISATGGKAVERAGAEGPELFFNLASDRLGSTALHLGHALFLTSLIAAMISFHNIIARYTFSLGREGVLPRAFGRTVPGTGAPKNGSLAQSVLGLVVIGVYAIAGWDPLVQLFFWGGTTGGIGVLLLITVTSAAVVGYFARNPEGEDFWHRLGAPVLGTVLLLVVTYLALTNIEILFGVEPGSTPTWAVPLAYAVLTLAGVTWALILRRTRPGTYQGIGMGARSGFSIRDTATEAHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W081</accession>
    <name>U5W081_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phenylacetic acid degradation protein paaD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362809.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20825"/>
    <dbReference type="EnsemblBacteria" id="AGZ42437">
        <property type="protein sequence ID" value="AGZ42437"/>
        <property type="gene ID" value="AFR_20825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4222"/>
    <dbReference type="eggNOG" id="COG2151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082133_1_0_11"/>
    <dbReference type="OrthoDB" id="1668114at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011883">
        <property type="entry name" value="PA_CoA_Oase4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02159">
        <property type="entry name" value="PA_CoA_Oxy4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="10"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="17327" checksum="679750F56C704C73" modified="2014-01-22" version="1">MVTAAAVAARVADPELPMLTLADLGILRDVDESDDGVVVTITPTYSGCPAMQTIQDDLTGALRRAGYARVEVRTVLTPAWTTDWITSAGRDKLATAGISPPGPAPRRGAGPVPLTLTPRRQMIACPQCGNAETEEIAAFSATACRALRRCPVCREPFEHVKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W097</accession>
    <name>U5W097_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362829.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20925"/>
    <dbReference type="EnsemblBacteria" id="AGZ42457">
        <property type="protein sequence ID" value="AGZ42457"/>
        <property type="gene ID" value="AFR_20925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4242"/>
    <dbReference type="eggNOG" id="ENOG5032MHZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1591063_0_0_11"/>
    <dbReference type="OrthoDB" id="1133467at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="17540" checksum="AE3BFFEBEB3AAE9F" modified="2014-01-22" version="1">MNQIRAEFLKLLTLPAVALTVTLTWVVTVVVPLSYARTGFVVFGILAATSEYEGGQVRTTLLAVPRRLDLVLAKTFVLAVTILPVAVVTVLIAGEGNPAYLLLITLLAAAVGTILRNALAAVVLLLSYFGVLGPILGDRISDSLWLPVAWTAGVSLLAAATFVGRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0B0</accession>
    <name>U5W0B0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coenzyme F420-dependent NADP oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363019.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21890"/>
    <dbReference type="EnsemblBacteria" id="AGZ42648">
        <property type="protein sequence ID" value="AGZ42648"/>
        <property type="gene ID" value="AFR_21890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4438"/>
    <dbReference type="eggNOG" id="COG2085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076368_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="1">
        <location>
            <begin position="2"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="21123" checksum="3883FB0561E31FF3" modified="2014-01-22" version="1">MRIAIIGTGNVGSALSTAAVKAGHTVTLAAAHPEHAADVAAATGAQAAGTAVEAAQGSDLVVLAVPAGAARGVAAELAGVLAGTPLVDATNPINASFDGLDIAGVSFAHELQEVVPGAPVVKALNTLFAGRYADPQENGRPLDAFIAADDEGAKARVAEFTASLGLRPVDVGGLRKARSLEEMAFLNITVNAANGWVWQTGWQLAGPTTATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0C3</accession>
    <name>U5W0C3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363039.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21990"/>
    <dbReference type="EnsemblBacteria" id="AGZ42668">
        <property type="protein sequence ID" value="AGZ42668"/>
        <property type="gene ID" value="AFR_21990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4458"/>
    <dbReference type="eggNOG" id="ENOG5031AT3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104624_2_0_11"/>
    <dbReference type="OrthoDB" id="2066547at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025492">
        <property type="entry name" value="DUF4383"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14325">
        <property type="entry name" value="DUF4383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="149" mass="15641" checksum="862045B465C2D27F" modified="2014-01-22" version="1">MVSRASAGVTPARSLVQTTALVVAGVFLLVGVLGFIPGITTDYSSMSFAGHHSEAKLLGVFQVSILHNIVHLLFGLAGFALARTVSGARSYLIGGGAIYLVLWLYGLVVGQDSAANFVPLNAADDWLHLFLGIGMIALGFLTTPKRVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0H4</accession>
    <name>U5W0H4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362966.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21620"/>
    <dbReference type="EnsemblBacteria" id="AGZ42594">
        <property type="protein sequence ID" value="AGZ42594"/>
        <property type="gene ID" value="AFR_21620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4383"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2434230_0_0_11"/>
    <dbReference type="OrthoDB" id="1204073at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="90" mass="9576" checksum="AC808F45E6B223BF" modified="2014-01-22" version="1">MYRAVLLDVYSEDARAYKPRPEPFLLALAQLGLTTADVVHVGDSPAADITGASALGIDTAFVRRGSRRLPEGLTATHTIGTLTELLPLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0S7</accession>
    <name>U5W0S7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363110.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22345"/>
    <dbReference type="EnsemblBacteria" id="AGZ42739">
        <property type="protein sequence ID" value="AGZ42739"/>
        <property type="gene ID" value="AFR_22345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4530"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_8_5_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="38708" checksum="09D0541EA5113455" modified="2014-01-22" version="1">MKFLLITLITHLDPTRSTRQRFREVVDNAVLGEELGFDGYGVGERHERPFISSSPPVVLSHIAARTSKIRLFTAVTTLSLLDPVRAFEDYATLDNLSDGRLELIIGKGNGAAQAELFHVTAEDQWDRNRESYELFRQLWRSPRVTWEGRFRPALRDAETWPRPLQQPIRVWHGSATSRASVDLAAQYGDPLFSANVTNPIEPYAELIRYYRSRWAHYGHDPAKALVGAGSAGYYAAPTSQEAVETYRPIFDARLEAFTRLGLTPVFPTLEDAIDRGSILVGSPQQIIDKIHRYHAEFGHEVMHLHADADGLTDKQHRATLELFQSDIAPELRRTIPSRPLGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W115</accession>
    <name>U5W115_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361763.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16835"/>
    <dbReference type="EnsemblBacteria" id="AGZ41645">
        <property type="protein sequence ID" value="AGZ41645"/>
        <property type="gene ID" value="AFR_16835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3415"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050006_2_2_11"/>
    <dbReference type="OrthoDB" id="1007505at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="21"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="275" mass="30527" checksum="3217F80F7285590A" modified="2014-01-22" version="1">MWTLSGFIDEISPDFTEQCRTAADLGLKYVELRSAWEVNILDLEPARLGVMKETLEAYDLEVSSIGSPIGKIFIDEDFPAHLDRMRHAADVAHLFGAPYIRIFSFFLRPGADPAAHRDEVIRRMRALARVAEESDLVLLHENEKEIYGDVPSRCLDIVRSVDSPHLRLAWDPANFVQVGVQPYSEGYAVLRPHVEYIQIKDARAADGSVVPAGEGDGEVARTMRALHHDGFDGFFSLEPHLSAGTAAGGFSGPDLFRTAWQAFTDLLKAEGIEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W178</accession>
    <name>U5W178_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23700"/>
    <dbReference type="EnsemblBacteria" id="AGZ43008">
        <property type="protein sequence ID" value="AGZ43008"/>
        <property type="gene ID" value="AFR_23700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4802"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="228" mass="23560" checksum="33432CED945B8B8A" modified="2014-01-22" version="1">MLTGRSAEKLAAAATGLRQRTGAQIDFLAGDVADPDSAEAIMREAAEWYDGPDVLVLNAGGPPPGPILEVGDDQWRAAFELLVLGPLRLARLALPGMAERGFGRVIVITSTAVRQPQPDLAASVVLRAAMTSAAKLLSREYASEGVTVNCVAPGPTATDRRHEILAARADRLGSTFEEQNAADTATVPAGRAGQPEEVGAAVAFLASAAASYVNGTVLTVDGGRTETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1E8</accession>
    <name>U5W1E8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_17265</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17265"/>
    <dbReference type="EnsemblBacteria" id="AGZ41731">
        <property type="protein sequence ID" value="AGZ41731"/>
        <property type="gene ID" value="AFR_17265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3501"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_7_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="24169" checksum="4624A092749E92ED" modified="2014-01-22" version="1">MFLAGAGVLAGIFGSVGGLASLASYPALLLAGLPPTAANVTNSVALVATTVGAAAGSGPELRGQGRRIGRLSVVSAVGGTAGAILLLTLPPGVFELVVPWLIAGASVVLLLGPRLRRPTDGDHRHASVGVFFIAVYSGYFGAAAGVLMLALLSAISTQSLARTNAAKTLITGASNAVAAVIFAVGGPVHWVAALALAAGSLAGGWLGPAVVRRLPERPLRIGIACAGLVLAAKLAVDAFRSGSWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1H1</accession>
    <name>U5W1H1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23010"/>
    <dbReference type="EnsemblBacteria" id="AGZ42872">
        <property type="protein sequence ID" value="AGZ42872"/>
        <property type="gene ID" value="AFR_23010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4663"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107911_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="15"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="22235" checksum="B6FEAB42DE5F4120" modified="2014-01-22" version="1">MITVMPSARRAEYADQTRRAILEAARSLFTDQGYFATRVEQIAAAARVAPATVYAVGGGKNGLLRTLIESAVTSDENARIFAEIEAATDPERLIAQVVGVSRTKFGQWSALMRQVVAAAPQEPAVRESMEIAHAGLRNGMRLTAERLAALGALRDGVDAAEAADILWLHLCNAAYFIRTDDLGWSLDKSEAWLNAVLPRELLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1P3</accession>
    <name>U5W1P3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363515.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23385"/>
    <dbReference type="EnsemblBacteria" id="AGZ42947">
        <property type="protein sequence ID" value="AGZ42947"/>
        <property type="gene ID" value="AFR_23385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4739"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090336_4_6_11"/>
    <dbReference type="OrthoDB" id="1832160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HATPase_c" evidence="1">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="14022" checksum="792D35E699825493" modified="2014-01-22" version="1">MDSLRLSFAHRTDYAGLRQHAREVLDRWQTPDLVDDTLLVITELVENVVQHTGDGGELVLHRCGDAVRVEVADSSSALPRAYGPDPRRIGGRGLLLVAAVSRDWGARPDGVGKIVWADVPLTSQASARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1R2</accession>
    <name>U5W1R2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-demethylubiquinone-9 3-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363558.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23485"/>
    <dbReference type="EnsemblBacteria" id="AGZ42967">
        <property type="protein sequence ID" value="AGZ42967"/>
        <property type="gene ID" value="AFR_23485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4759"/>
    <dbReference type="eggNOG" id="COG0500">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071058_1_1_11"/>
    <dbReference type="OrthoDB" id="1865374at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="62"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="21914" checksum="1BE5776A55CB5858" modified="2014-01-22" version="1">MTVITTAPVFDSALRDAGAGQSAIVTLLDDRGVAHRIDAAAWCRNHLPGDRGLLARCAGPTLDVGCGPGRLTSALNRLGHPALGIDVSPAAVRLARARGATVLLRDVFGPLPGHGRWRHVLLADGNIGIGGDPGALLSRCRDLLAADGRVHVELSPPGTRSWSGEARIAGEPFRWAQLAAGDLAGPAAASALHIAATWTEANRWFATLTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1X6</accession>
    <name>U5W1X6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363818.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24810"/>
    <dbReference type="EnsemblBacteria" id="AGZ43228">
        <property type="protein sequence ID" value="AGZ43228"/>
        <property type="gene ID" value="AFR_24810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5026"/>
    <dbReference type="eggNOG" id="COG2337">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2244171_0_0_11"/>
    <dbReference type="OrthoDB" id="1935622at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003477">
        <property type="entry name" value="PemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011067">
        <property type="entry name" value="Plasmid_toxin/cell-grow_inhib"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02452">
        <property type="entry name" value="PemK_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="104" mass="11584" checksum="6A538DD787C8801A" modified="2014-01-22" version="1">MKRGEIWTIGSRTDLRYRVLVLSSDSYNERNNASPYCAPIVRQRGATELPPYAVALTEQDPITGVVVVNRMRRLPASVGAERIGMVTGSSMARLAEAMRELFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W240</accession>
    <name>U5W240_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24615"/>
    <dbReference type="EnsemblBacteria" id="AGZ43189">
        <property type="protein sequence ID" value="AGZ43189"/>
        <property type="gene ID" value="AFR_24615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4987"/>
    <dbReference type="eggNOG" id="ENOG5033CYP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137402_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="154" mass="16465" checksum="043D7C2B309BBC4D" modified="2014-01-22" version="1">MTPEEVLSRLEAPLSLRKRIAYLTVALAGLTASALIGLLWATEPDLPLRTEVAFAVLVTIGLCWAAFGGWAVTRRTPLFARDRVIAAWLGAGAWLTLTIGASAITILRHEFEPAVAGIVLTLGVLAAVNLRAARRARAALTRRKTELSPSAGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2P1</accession>
    <name>U5W2P1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364257.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26180"/>
    <dbReference type="EnsemblBacteria" id="AGZ43498">
        <property type="protein sequence ID" value="AGZ43498"/>
        <property type="gene ID" value="AFR_26180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5306"/>
    <dbReference type="eggNOG" id="ENOG5031WG5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_206290_0_0_11"/>
    <dbReference type="OrthoDB" id="2083438at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="67" mass="7440" checksum="4D96478F132C2860" modified="2014-01-22" version="1">MIFGLIRRYVLMAVAIPLAAAGARKLSQTLEARRGPSRGTRLLRQGADTLQGRFGKQKKRSRFALGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2W9</accession>
    <name>U5W2W9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26075"/>
    <dbReference type="EnsemblBacteria" id="AGZ43479">
        <property type="protein sequence ID" value="AGZ43479"/>
        <property type="gene ID" value="AFR_26075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5285"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_9_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="414" mass="45479" checksum="7E3203BEFFCC5068" modified="2014-01-22" version="1">MTFVAGNLTGAAIAACLFVFGPLAGPEELESGELVILSGQDDSAGGQRQQLVTIWNDTHPRNQARIVTLPEAADGQYAEMVNRAGDGGTDIFNLDVAWTARFAAPPSGRRLIRPIDESLLAERPDQAFMTKPLATCRYADQLWALPFNTDAGLLYYRTDQGLKPPFDWSKIRAAGTKPGFKATWTGQLNSYEGLTVNFLETVWSLGGDLAVAPDGRVTLDLAKWDEAAKLLTPSRDAKPGLVLPDSVSFEETSSRSAFRDGQTMFMRNWPVAYRAMRTAEPAGKPFAFDVTRLPGDSVLGGQNLAISERTTKPRAAQALIEFLTNERSQRLLFDNGGFAATRAAVYEDEAIKQKYAYLPRLREAIDTARLRPVSPNYVTFSRVLNERVHEVLVGRSPALPRDLADQLTRALQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2X3</accession>
    <name>U5W2X3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome subunit beta</fullName>
            <ecNumber evidence="3">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Proteasome core protein PrcB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">prcB</name>
        <name evidence="4" type="ORF">AFR_26405</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein degradation; proteasomal Pup-dependent pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26405"/>
    <dbReference type="MEROPS" id="T01.005"/>
    <dbReference type="EnsemblBacteria" id="AGZ43543">
        <property type="protein sequence ID" value="AGZ43543"/>
        <property type="gene ID" value="AFR_26405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5352"/>
    <dbReference type="eggNOG" id="COG0638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_2_0_11"/>
    <dbReference type="UniPathway" id="UPA00997"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_B">
        <property type="entry name" value="Proteasome_B_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022483">
        <property type="entry name" value="PSB_actinobac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03690">
        <property type="entry name" value="20S_bact_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0645">Protease</keyword>
    <keyword evidence="3 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5005089419" description="Removed in mature form; by autocatalysis" evidence="3">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023481652" description="Proteasome subunit beta" evidence="3">
        <location>
            <begin position="54"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="29029" checksum="41326C9B8E8408D7" modified="2014-01-22" version="1">MAAGFDPSGRLPDVFTNAGTSSFTQFLSLAAPEMLPGRRPLPPGLSADIAPHGTTIVAIATAEGVVMAGDRRATMGNLIASRDIEKVHPADAYSLIGIAGTAGIGIELMRLFQVELEHYEKIEGAMLSLDGKANRLAAMVRGNLGAAMQGLAVVPLFAGFDLSPSDPSRAGRIFSFDVAGGLYEETGYDAIGSGSLFAKSALKKKYRAGVSTQDAVRLAVEALYDAADDDTATGGPDLTRKIYPVVMTATADSTHRLTEAEINAVAEQVVAGRMENPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W317</accession>
    <name>U5W317_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20245"/>
    <dbReference type="EnsemblBacteria" id="AGZ42321">
        <property type="protein sequence ID" value="AGZ42321"/>
        <property type="gene ID" value="AFR_20245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4105"/>
    <dbReference type="eggNOG" id="ENOG502Z9UU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065986_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="33192" checksum="ACFFAF000A7094F8" modified="2014-01-22" version="1">MDALEITCDESGYEGEKLIGTTTDVFAHAGVRLGAEAARACLTELRDRIRSPATQYKATHLLREKHRPVLVWLLGPSSPLFRRAHVYLIDKVYYVLGKLTDVLVADPAGAGLSGDGPARRLSAELFAGRPGHDYLVAANNLLRGKDRHDVQGPVETFYRLAGELGGRSRAEAFRVRLRDDPALSVLDPLIPAVVRAVGRWGADGTPVSILHDRQTTLPEERVARIRRLLAAPLRLEGLRLAAAEDEPRIQLADILAGTVRKIAQDQLHGAGDTELVTLIRPYLDPYSIWGDRRSWALLSARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W361</accession>
    <name>U5W361_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364177.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25965"/>
    <dbReference type="EnsemblBacteria" id="AGZ43457">
        <property type="protein sequence ID" value="AGZ43457"/>
        <property type="gene ID" value="AFR_25965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5264"/>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_1_11"/>
    <dbReference type="OrthoDB" id="1583074at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="29135" checksum="5D32D9A96A0ABDA1" modified="2014-01-22" version="1">MAAVAEAFDRDLEGDFARIEKLIGAARADGVQLLALPEAALGGYLAHLDGGADQPPALRLDGPEIARLVELAGDMVVCAGFCEADGERRFNSVVCVGGGRILGRHRKVHQPLRENASYDSGDEFAAFDTPVGRLGLLICYDKAFPESARSLALDGAEIIVCVSAWPGSRTDAPADLADDRWTRRFDLFDRARALENQVVWVSANQSGTFGSLRFVCSAKVVDPGGDVLATTGTAPGMAVADVAVTEALTTARRSMGHLRDRRPDAYPKLAPAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W365</accession>
    <name>U5W365_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25990"/>
    <dbReference type="EnsemblBacteria" id="AGZ43462">
        <property type="protein sequence ID" value="AGZ43462"/>
        <property type="gene ID" value="AFR_25990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5269"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006909_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024000">
        <property type="entry name" value="CHP04046_FMN-dependent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04046">
        <property type="entry name" value="MSMEG_0569_nitr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="440" mass="48576" checksum="A2B7F66F43D10F7D" modified="2014-01-22" version="1">MKIAGTHHPVVVIGGGQAGLSMSWYLRQRGIDHVVFERERVGHEWRERRWDSFCLVTPNWQCQLPGFGYTGDDPDGFMLGADVVGYLEKYAASFGPPLAEGVAVTRLRRTPSGVFDLVTTAGNCTADQVVVATGPYHNPAVPRIAERLPDDVAQLHSSQYRSPERLPEGAVLVVGTGQSGCQIAEDLHLAGRRVHLAVGSAPRAARRYRGRDTLAWLDEMGHYARGIDEFADADEVRLRANHYMTGRDGGRDIDLRAFARTGMRLYGRLRAIDDTTVSFADDLAANLDNADAVAEGIKDAIDRYIVAAGVEAPAEDRYVPVWEPSGEPAELDVRDVSAVVWATGYHRDHRWIEVPVFDGRGYPTHDRGVTSCPGLYFLGLPWQWTWGSGRFAGVARDAGYLADRVTATRRQRVTEEVSWLAGTPTETYPDLDWFTPRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W385</accession>
    <name>U5W385_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lysozyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27035"/>
    <dbReference type="EnsemblBacteria" id="AGZ43668">
        <property type="protein sequence ID" value="AGZ43668"/>
        <property type="gene ID" value="AFR_27035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5479"/>
    <dbReference type="eggNOG" id="COG1705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4991">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_722865_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002901">
        <property type="entry name" value="MGlyc_endo_b_GlcNAc-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003646">
        <property type="entry name" value="SH3-like_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01832">
        <property type="entry name" value="Glucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08239">
        <property type="entry name" value="SH3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00047">
        <property type="entry name" value="LYZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00287">
        <property type="entry name" value="SH3b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51781">
        <property type="entry name" value="SH3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666043" evidence="1">
        <location>
            <begin position="41"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="SH3b" evidence="2">
        <location>
            <begin position="42"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="386" mass="41132" checksum="E909B0925BBD300A" modified="2014-01-22" version="1">MPAAMKGTFMLHTARRLLTAPLLLAALGAGLVVAPTAASAAGVSATIKVGSTLQVRSAPSLSAKIVGTVRNKQKVTVSCFVTGQKVRGAVRTTTAWDRLSTGKYVSHGYVKASRSIPRCAVSSPTAVPSKTKTSGTSRYVIGTVKSVDSKVNLRSGPSTDSAVTGTVTNGTKIDLTCAVTGSLVDGTVRSTNQWDRTPTGSFISHAYVYSGTLPVCAGSTTPTPSVTLTPAEFIRASVPGAQRGWREYGVPPSVTIAQAILESGWGRSGLASVDRNYFGIKCQNGSYGKLANGCHVYRTNECTKTGTCFSTSASFRTYATQSHSFRDHGNFLKVNSRYAGAFEHTRDANKFIWKVWKAGYATDPNYYTKVTGIMASYDLYQYDTWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3E2</accession>
    <name>U5W3E2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364514.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26840"/>
    <dbReference type="EnsemblBacteria" id="AGZ43629">
        <property type="protein sequence ID" value="AGZ43629"/>
        <property type="gene ID" value="AFR_26840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5439"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_1_11"/>
    <dbReference type="OrthoDB" id="1439833at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="26"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="31033" checksum="E634EEB8A5D50B13" modified="2014-01-22" version="1">MFEGFDLDHIDVGAATLRVRHGGSGPPVVLLHGHPRTHVTWQHVAADLAADHTVVCPDLRGYGRSSKPPAGEDFSGYSKRAMAGDIVALMTELGHSRFAVAGHDRGCYVAMRLALDHPYAVSHLAVLDGVPIGEALSRAGSRFAQLWWHWFFLGQTAKPAERVINADPEAWYGLGDSVPPEEREAIYDPETVHAMCCDYRAGLRADREADDADWAEGRIITCPTLFAWSTRDDMEELYGDPLSIWRDWAADVEGVAIDSGHHMAEENPQALTTALRALLRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3S8</accession>
    <name>U5W3S8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type 11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21455"/>
    <dbReference type="EnsemblBacteria" id="AGZ42561">
        <property type="protein sequence ID" value="AGZ42561"/>
        <property type="gene ID" value="AFR_21455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4352"/>
    <dbReference type="eggNOG" id="COG0510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_580920_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="2">
        <location>
            <begin position="37"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="305"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="470" mass="50347" checksum="CF72A1649217E57F" modified="2014-01-22" version="1">MAESFGTDAGLYDRTRPRYPDELVDRIVGGRRGLQVLDVGMGTGIVARQLQAAGCVVLGVEPDERMAAFARRTGCVVEVATIEAWEPAGRVFDAVVSGQAWHWVDPVAGAVQAARVLRPGGRLAVFWNAAQPPPELAEAFGDVYRRVLPQSPMTAGVDSKLTAAEMYALMAGKAADGMRAAGGFGAVEQWRFEWEQAYSRDEWLAQVTTQGGQSQVPARVREELLEGLGAAIGDGFTMHYTTVVATAVREVPLVGGFVGAAVRVGDTVRKAPPPDPGFVRGLLTTFAGLGWAGAPRFLGTDEQGREVLSFVEGDVPSGPVSDEALAAVARLVREFHDLTGGVVCHHDLSPGNTVFRDGMPVAFIDWDLAGPGLPVQDLAHVCWQFVPLGPGADVAEAARRVRVVADAYGWVDLPLLVETVLWWQDRCWRGIAAGADDGVPAMVRLRDAGVVESVRAAYAWTLERRAELSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4B9</accession>
    <name>U5W4B9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UDP-glucuronosyl/UDP-glucosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363160.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22480"/>
    <dbReference type="EnsemblBacteria" id="AGZ42766">
        <property type="protein sequence ID" value="AGZ42766"/>
        <property type="gene ID" value="AFR_22480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4557"/>
    <dbReference type="eggNOG" id="COG1819">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_628262_0_0_11"/>
    <dbReference type="OrthoDB" id="1485440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="421" mass="44269" checksum="A2DDBA2B4F379267" modified="2014-01-22" version="1">MTLLVISPDYASHLLPLATLATAWRDAGERVVVATGPATAPIVTSFGYERTDLSLGRNSNAGVIQPEDDALRGFFAATRRGLVETLLHQARARRSDLLWDPVGRAHAVLKAVEEVKPDRIIVDHLAFSARLGLTAFDVPHADVVLGHPSALPVGGEVYGYPTDWPAAFDPSPMALARLHQVCRDVRDAFTQEWNAALRVLSPSAKPSDDALAAHGAPLLLNYPEALHDPARTTLLPPHAFLGSAVRTETVPADIATWLDADDRPLVYVSFGSFLSARGDVLAKVLVGLRSLDVRVAVATGSARALPALPDTWLARTHLPQTALLARAALAVTHGGNNSVTEALTSGVPMLVLPFSTDQFAGAASVEASGTGAALDPNKVTPIAIADAVRELLAGSSPAVTAELGEQLRAKPGPLLARAALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4F9</accession>
    <name>U5W4F9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560366.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28840"/>
    <dbReference type="EnsemblBacteria" id="AGZ44029">
        <property type="protein sequence ID" value="AGZ44029"/>
        <property type="gene ID" value="AFR_28840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5846"/>
    <dbReference type="eggNOG" id="COG4292">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045667_0_0_11"/>
    <dbReference type="OrthoDB" id="886435at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010640">
        <property type="entry name" value="Low_temperature_requirement_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840">
        <property type="entry name" value="PTHR36840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06772">
        <property type="entry name" value="LtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="399" mass="42891" checksum="AB1D5C14242AB830" modified="2014-01-22" version="1">MSGQRHRSWHTPMQARSATEAHRAATPLELFFDLCFVVAVAQAALPLHHAISENHIAEGVGTYLMVFFAIWWAWVNFTWFASAYDTDDDVYRITTLVQIAGVLVLAAGVETAFEDGTFTAIVLGFVIMRLALVAQWLRAARSDPDHRKVALRYAVGISVTQALWLVWLTVPESWSVPLFLLLALAELLVPALAEYAPGGPTTYHPHHITERYGLFTIIMLGEAVAATTIAVKTGLDEGKYLPDLVVVAASGLVIVFALWSLYFERSAHRLLSTQRTALVWGYGHYLILGAAAAVGAGLGVVVDHTTHHAHISGVVAGYAVAVPVVVFLLAVWLLHVRPVQTGARLAAYPISSVLVLLAPLGPAPVTVIAVLLAMLVVVTVAGSRRGVPAAEAPQATSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Y8</accession>
    <name>U5W4Y8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23860"/>
    <dbReference type="EnsemblBacteria" id="AGZ43040">
        <property type="protein sequence ID" value="AGZ43040"/>
        <property type="gene ID" value="AFR_23860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4833"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28770" checksum="302DB35CB0174A30" modified="2014-01-22" version="1">MNRTALVTGASSGIGQATAAALAARGYRVLGTSRHPESLAEGQRAPGVEYLPLDLTDDNSVDALADRLTEVDVLVNNAGESQSGPFEELPADALRRLFQVNVVAPVRLTQLALPGMRKRGYGRVVMVGSMLASFPLAYRSSYVASKAALKGFSNAARHELSPFGVWLTTVEPGSINTGISERRTRYVNPDSPHRPDFETMLSALDRNERDGVPAGRVASTIVQAIEAARPKPRYAVGSNAPLVFALRRILPAGLTEKLIHRRYGLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W546</accession>
    <name>U5W546_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="6 12">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AFR_29355</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 12">
            <text>a dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9517"/>
            <dbReference type="Rhea" id="RHEA-COMP:9527"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>a dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:9517"/>
            <dbReference type="Rhea" id="RHEA-COMP:9527"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="6 12"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29355"/>
    <dbReference type="EnsemblBacteria" id="AGZ44132">
        <property type="protein sequence ID" value="AGZ44132"/>
        <property type="gene ID" value="AFR_29355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5949"/>
    <dbReference type="eggNOG" id="COG4346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021079_0_0_11"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="GlyclTrfase_39-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="Glyco_trans_39/83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="PTHR10050"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="PMT" evidence="13">
        <location>
            <begin position="54"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="PMT_4TMC" evidence="14">
        <location>
            <begin position="328"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ44132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="525" mass="58680" checksum="621500FA863ADDBC" modified="2014-01-22" version="1">MGAVLVLAVGEGMMVLTATTERPTPARAVPELVRRRLATLENWLNPYSWLVTGIIVVIAAILRLNGITTRKGYIFDEVYYPTDAWDMLQHGVEWDPEKTGGPAFVVHPPLGKWLIALGEKAFGNNELGWRFPAAIAGTLMILILIRVAYRMFHSIVLAGTAGLLMTLDGFQLVLSRTSLLDIFLGLFVLASFAALLLDRDHYRRRLLRSLERAEDPAVPRAVPWWLLMAGALLGLACGVKWSALFFLPFFAVLIIAWRVQARRSAGVRRPLRAGLVGDLGWGLLSSVLVVVFYLATWTGWFVTSGGWDRHYREQNGLSEPPVIGALLNLMHYHQSAFEFHSNLSSPHTYQSWPWQWLLLGRPVAFDWKSNAGNCGAASCVRETLLLGTPILWWSFLPALAALIWFGLSRRDWRAWAILVPVAAGLLPWFYYAVKDSRTMFAFYALPALPFLILAVVYVLGAIMTPPEGMVQGPGRTDRQLLGVIVAGAYVVLVALCFAYFHPIFVNGLLSYEDWAARMWLGSRWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5B1</accession>
    <name>U5W5B1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_30370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560671.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30370"/>
    <dbReference type="EnsemblBacteria" id="AGZ44334">
        <property type="protein sequence ID" value="AGZ44334"/>
        <property type="gene ID" value="AFR_30370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6149"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="144"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="22891" checksum="9045B6A87E3EE171" modified="2014-01-22" version="1">MRVVIAEDNPLLRDGIALLLGEHSIEVTAAVPDAEALLAAVAADPPDAAVVDVRMPPTHTDEGLRAALTLRSAYPEMGVLVFSQWVETSYARQLLSVNVGGVGYLLKDRIVSTGEFVGALQRVAAGGTALDPEVVRQLLAAPAVDAGLARLTGREREILGLLAEGRSNVAIADALHLVERSVEKHVTAIFTKLDLPQARTDHRRVLAVLRYLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Z2</accession>
    <name>U5W5Z2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-N-acetylhexosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_30860</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30860"/>
    <dbReference type="EnsemblBacteria" id="AGZ44432">
        <property type="protein sequence ID" value="AGZ44432"/>
        <property type="gene ID" value="AFR_30860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6247"/>
    <dbReference type="eggNOG" id="COG3525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007082_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102148">
        <property type="term" value="F:N-acetyl-beta-D-galactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015882">
        <property type="entry name" value="HEX_bac_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="PTHR22600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02838">
        <property type="entry name" value="Glyco_hydro_20b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="SSF55545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_20b" evidence="7">
        <location>
            <begin position="2"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_20" evidence="6">
        <location>
            <begin position="116"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="480"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="522" mass="56458" checksum="F88A854BE6D2DDAB" modified="2014-01-22" version="1">MIPRPSAVTHRAGAFELHPSLPVVAEPGLEGVASWLRGLLPAGGISLRHAPDLPAEGYLLDVTERGIDIRGGSAAGVFYGVQTLRQLLPASALRRARIGDSPLLVPAVRIEDAPRFGWRGVLLDVARHFMPVAGVLRFIDLAAFHKLNVLHLHLTDDQGWRIEVPGRPRLTTVGAWRPATMLGSRIHDRYDTRPHGGFYTADDLLEIVAYAAERHVTVVPEIDMPGHMQAAIAAYPELGNGFAGGVRTSWGISPHVLNVSDTALDFCRAVLDHVCEIFPGELIGIGGDECPAGEWDSPAARERIAAEGLAGPHELQAWFTARMAEHLKNHGRRVLGWDEILAGGAPAGATIAAWRGAEPAVLAARAGHDVVSCPDTSVYLDYRQSTGPGEPTPVGTLLTLADVYAFDPVPAGLSDAEAARILGGQANVWTEHLESARRVDYQTYPRLCAFAETVWGPPRRDFAGFTTRLGPHLERLDALGVNYRPPSGPRPWDARPDAPGHPRDLADRLAELHEMTADLRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6B1</accession>
    <name>U5W6B1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF397 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560997.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32020"/>
    <dbReference type="EnsemblBacteria" id="AGZ44664">
        <property type="protein sequence ID" value="AGZ44664"/>
        <property type="gene ID" value="AFR_32020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6482"/>
    <dbReference type="HOGENOM" id="CLU_131550_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007278">
        <property type="entry name" value="DUF397"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04149">
        <property type="entry name" value="DUF397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF397" evidence="1">
        <location>
            <begin position="9"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="7859" checksum="4774CABBCAE30D43" modified="2014-01-22" version="1">MTDSIWTDAVWHKSSRSNGGGQCVEIAVHADLVAVRDSKDPDGAMLIFPARTWQSFLGWIVDNDELTNLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6B7</accession>
    <name>U5W6B7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26170"/>
    <dbReference type="EnsemblBacteria" id="AGZ43496">
        <property type="protein sequence ID" value="AGZ43496"/>
        <property type="gene ID" value="AFR_26170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5304"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_7_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15160" checksum="49A058333F2C5622" modified="2014-01-22" version="1">MNLGLLCFVAYRAMESGVLEALAAAGHGDLTAAQGRVFARIGENGTRVTELAEQAQITKQTAGFLVDQLERAGYVERVPDPSDARARLVRIAPRGSEAVKVARAAEAEIEATWTRHLGKQSAAQLRRSLTKLREITDPYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6H3</accession>
    <name>U5W6H3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560930.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31685"/>
    <dbReference type="EnsemblBacteria" id="AGZ44597">
        <property type="protein sequence ID" value="AGZ44597"/>
        <property type="gene ID" value="AFR_31685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6413"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848955_0_0_11"/>
    <dbReference type="OrthoDB" id="821953at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666127" evidence="1">
        <location>
            <begin position="32"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="34001" checksum="AAF1B4E25335C3C0" modified="2014-01-22" version="1">MKAVTRVAGNISLAVAGVAAGALFQTAPAAAATPAMPALVAYVRSGDVWVSKGATESKLTTGGGWSRPRFSPDGASIALLKGGQLWTMKADGTAKRRLTTRAASGPSWAPDSSSIAFASLSCSGGPGVYRISATTVNAAPEVVFPAYCRDEELPAEPVTARTAQPAGSLSDRLRADDAVAWSPDGTKVAFRGGDCESTYDACLTIGTVATGTEKTVAAYGGGSLQTTGFAVIPHWRTDGAKLAWTAYQKGETLADNQPLHVVEYDTLTGTKRTIGANQDRELAYVDTTRGVATGTYRNGSWIMLVNLATGVRTPFHQGSQPSVQPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6M7</accession>
    <name>U5W6M7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NMT1/THI5 like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32755"/>
    <dbReference type="EnsemblBacteria" id="AGZ44809">
        <property type="protein sequence ID" value="AGZ44809"/>
        <property type="gene ID" value="AFR_32755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6629"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010067">
        <property type="entry name" value="ABC_SsuA_sub-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01728">
        <property type="entry name" value="SsuA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMT1" evidence="2">
        <location>
            <begin position="39"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32562" checksum="7D2DC13A7778FD40" modified="2014-01-22" version="1">MLAACGDTAAEANGRKKVRLTYGPISVPKTRGVFEKTLNDQGIDVEWVGPFPNHAPTLQAVATDTADFSFGGSSTPADQALLSGNRLKYVAWATATPRLSSILVLPDSGINSVKDLAGKNVAVNKAGLGEFLLVAALEKYGVPRDSVTFTYLNPPDAAPAFGSGKVDAWGIWSGPMELAEVQYGAKRLFTDGDELDKQIDFSTYLVREDYAKKEADTIRKVIAAYKAEADWVNTNQAEAQKIGNEVSKYPQAVVDKLVAQNVQTTWSLITDDGIAQLQAGADWLSDRKVLSGKINIADHAVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6T7</accession>
    <name>U5W6T7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LuxR family two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33030"/>
    <dbReference type="EnsemblBacteria" id="AGZ44864">
        <property type="protein sequence ID" value="AGZ44864"/>
        <property type="gene ID" value="AFR_33030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6684"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="13"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="159"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="23846" checksum="93F29B28B6F443C9" modified="2014-01-22" version="1">MTTAGADPGAKVRVLLVDDDALVRAGLTMMLGAFDGIEVAGSASDGTEVAAAVNTHRPDVVLMDIRMPGMDGLTATETLRARRDPPEVIVLTTFDTDAHVLRAMRAGASGFLLKHTPPVEIARAVRRVAAGEPMMSPEVLRRMMTFVAAAGADPGRERARAALSRLSPGEREVALLVAQGRTNQEIGTALRMSTATVKAYVSRILTKLELTNRVQVALLVHDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W772</accession>
    <name>U5W772_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_32970</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32970"/>
    <dbReference type="EnsemblBacteria" id="AGZ44852">
        <property type="protein sequence ID" value="AGZ44852"/>
        <property type="gene ID" value="AFR_32970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6672"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010347_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="123" mass="13155" checksum="F9FE4D158078E935" modified="2014-01-22" version="1">MDGDAVLLVDHRNAQLWLPPGGHVDPGEDPAVTAARELEEELGVTGAAATPVFLTVTVTVGLDAGHTDVSLWFPADVSRDEPLTVDEGEFAGVRWWTRAEIAAADPARFDPHLGRFLKKTAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W789</accession>
    <name>U5W789_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NmrA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561209.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33070"/>
    <dbReference type="EnsemblBacteria" id="AGZ44872">
        <property type="protein sequence ID" value="AGZ44872"/>
        <property type="gene ID" value="AFR_33070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6693"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_10_4_11"/>
    <dbReference type="OrthoDB" id="1616572at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="28106" checksum="7DE919D6FE8CA019" modified="2014-01-22" version="1">MIVVTGASGQLGGRVAARLAEAGEQQRLVVRDPARAPALAGAEVAVAEFGDPDAVRRALDGAGTVLMVSASETPDRVDRHKAFVDAAVAAGAGHLVYTSFFGAAPDAVFTLARDHWATEEHIRASGLPFTFLRDNLYADFLPFLVGDDGVIRGPGGDGRAAVVAQDDIADAAVAVLRDPAAHTGRTYDLTGPEALSFADLAAILTTSTGRAVTYHDETLAEAYASRAKYGAPDWQVDAWVSTYTAVAAGQLEAVSTAVADLTGRPATPFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7I7</accession>
    <name>U5W7I7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein adenylyltransferase SelO</fullName>
            <ecNumber evidence="9">2.7.7.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">selO</name>
        <name evidence="11" type="ORF">AFR_28275</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the transfer of adenosine 5'-monophosphate (AMP) to Ser, Thr or Tyr residues of target proteins (AMPylation).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + L-seryl-[protein] = 3-O-(5'-adenylyl)-L-seryl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:58120"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:15073"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:142516"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + L-threonyl-[protein] = 3-O-(5'-adenylyl)-L-threonyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54292"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13847"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:138113"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28275"/>
    <dbReference type="EnsemblBacteria" id="AGZ43916">
        <property type="protein sequence ID" value="AGZ43916"/>
        <property type="gene ID" value="AFR_28275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5731"/>
    <dbReference type="eggNOG" id="COG0397">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010245_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PTHR32057"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="376"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="417"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="115"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ43916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="559" mass="58984" checksum="A635CEE23566A369" modified="2014-01-22" version="1">MILGNRFARELPELALTWQADPAPAPQILVLNEPLALELGLDPADLSSGLLAGTDVPQGATPVAQAYAGHQFGGYSPRLGDGRALLLGELTTPDGSLRDLHLKGSGRTPFARGGDGLAAVGPMLREYVISQAMHALGIPTTRSLAVVSTGRTVQRDTPLPGAVLTRVAASHLRVGTFQYARATGNLDLLRRLADHAITRHHPAAADAERPYLALFESVLAAQASLIARWMLAGFIHGVMNTDNMTISGETIDYGPCAFLDTYDPATVFSSIDTGGRYAYGNQPAIAQWNLARLAESLLPLIDDNEEQAVTLAVAALETFGHRYRDAYLTGLRAKLGVPEGPETDALINELLPLLKQNSVDYTSFFRALSAAARGTTEPARTLLSGPDTLSAPSLLAGRDRLAANSPLTEREDSEPSTTEPGSNAPGNARQGDAQPSGTAPASTGPGGTRPGDTQPSGTAPASTALGDEQAGVAADGGWLDRWLALKPDAEAMDRVNPVYIPRNHLVEEALTAASDDGDLKPLEQLLEAVTDPFQERPGLERYAAPAPKDFGPYQTFCGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7P0</accession>
    <name>U5W7P0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydrofolate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560303.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28525"/>
    <dbReference type="EnsemblBacteria" id="AGZ43966">
        <property type="protein sequence ID" value="AGZ43966"/>
        <property type="gene ID" value="AFR_28525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5783"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_1_3_11"/>
    <dbReference type="OrthoDB" id="1306955at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="4"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20138" checksum="7E53CBB872B5014B" modified="2014-01-22" version="1">MTELVLKMSISLDGYVARPDGSNDWIFPSYTPDATAWTVETLRGAGAHVMGSVTYRAMAAHWPHDTSDFAAPMNDTPKVVFSETMTASEWGDTHFISGELAEGIATLKKDRAPDYLLAHGGAGFVRSLCRTGLIDEIRLLVHPVILGEGERIFLDPMDLTTVATKAFSGGAVAHVLRPAARALSGAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Y5</accession>
    <name>U5W7Y5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34295"/>
    <dbReference type="EnsemblBacteria" id="AGZ45117">
        <property type="protein sequence ID" value="AGZ45117"/>
        <property type="gene ID" value="AFR_34295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6939"/>
    <dbReference type="eggNOG" id="ENOG502ZIDU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1275453_0_0_11"/>
    <dbReference type="OrthoDB" id="1856623at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665647" evidence="2">
        <location>
            <begin position="30"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="240" mass="24493" checksum="58B1F327DE5328A0" modified="2014-01-22" version="1">MGKAPTMRFLPVIGTLLAAAIVTAGCSDGVERLDGAATPIGAVSASPSPAPSVTSPSSAPSSSPSPSPSESPSKSPSKSPPGSSPTSSSPKSRPTVLGPNGLGALKLGMSQKEAEATGVLTAFKGKINEGCNQPAHVRYASGINPGSDGMVYYSPGVLGIQIIDAYTGVRTPEGITLGSSAKAMRRAYPDWRNATEEDPTADGRGYAEVAGNGKARYRIVTANGLVVELTLQWVNQNCYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W836</accession>
    <name>U5W836_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_35265</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35265"/>
    <dbReference type="EnsemblBacteria" id="AGZ45309">
        <property type="protein sequence ID" value="AGZ45309"/>
        <property type="gene ID" value="AFR_35265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7135"/>
    <dbReference type="eggNOG" id="COG0531">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017999_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="664"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="664"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="700" mass="75940" checksum="2F319D0CF932D84D" modified="2014-01-22" version="1">MPGAADGVASAAVLLALVASSTSLAKRLLLGRPFRSDRLQHTLLPKRIALPVFASDALSSVAYAPDEILLTLSIAGAGAFIYSPWITLAVAVVMVTVVASYRQNVHAYPSGGGDYEVATVNLGPRFGVAVASALLVDYILTVAVSVSAGVANLGSVVPFVAEHKIGIAIAAIVVLGALNLRGLRESGTAFAIPTYAFIFVMLGMIITGLVRVFILGQDLRAPSADLIISAEEHHLTSFAFVFLLLRTFSSGCAALTGVEAISNGVPAFKPPKSKNAATTLLLLGGIAILMLVGIVMLARATHLQYVEDPLTQIAGGPANYVQKTVTTQLGETVFGNGSILLYLLGAVTALILFLAANTAFNGFPVLGSILAQDRYLPRQLHTRGDRLAFSNGIIFLAVAAMVLVVAFDAEVTRLIQLYIVGVFVSFTLSQAGMIRHWNRLLRTQRDAEARRRMIRSRTINTFGMALTGAVLIVVLITKFLLGAWIAIAAMLVIYVLMLGIHKHYTRVAQELEPSDERPVLPSRNHAVVLVSKVHKPTLRAVAYAQATRPDTLTAVTVNVDDKDTRRIQDEWERRQIPVPLTVVDSPYREITRPIIDFVKSIRRGSPRDVVTVFVPEYVVGRWWENLLHNQSALRIKGRLLFEPGVMVTSVPWQLQSSQDRDLERLDRDLSRAPARGPRTRRTDLPVAAEPAAPETREEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W841</accession>
    <name>U5W841_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34605</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34605"/>
    <dbReference type="EnsemblBacteria" id="AGZ45177">
        <property type="protein sequence ID" value="AGZ45177"/>
        <property type="gene ID" value="AFR_34605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7001"/>
    <dbReference type="HOGENOM" id="CLU_1599197_0_0_11"/>
    <dbReference type="OrthoDB" id="2182306at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="18722" checksum="A16A6ACFD9314F2E" modified="2014-01-22" version="1">MRAAALSVAVTMIVADGKGGHTALLIPRSDKVLTHPGFVHVAPSGVFAPTEQHWRLLKAVGRKQVALRYCGISVPLLTLRPEIDVLIFVRDPEWFREEMRRARRKGLREFQPNWEYRSARDVIWLRLKPDFTPYEDDVIDPARMVPHAAASLSLATRVARQMGTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8L1</accession>
    <name>U5W8L1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560603.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30030"/>
    <dbReference type="EnsemblBacteria" id="AGZ44266">
        <property type="protein sequence ID" value="AGZ44266"/>
        <property type="gene ID" value="AFR_30030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6082"/>
    <dbReference type="eggNOG" id="COG2337">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097054_0_0_11"/>
    <dbReference type="OrthoDB" id="1924690at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003477">
        <property type="entry name" value="PemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011067">
        <property type="entry name" value="Plasmid_toxin/cell-grow_inhib"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02452">
        <property type="entry name" value="PemK_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="18256" checksum="8865D71FB8833F77" modified="2014-01-22" version="1">MSRLLKGIASTLRRVAGRPAPGPQGTSHPAPPVPQQVPTARRGRHLEYSPNLDGDADPGEIVWTWVKYEDDPSQGKDRPVLVVGRNGRTLLGLMLSSQHERDGQHGWLGLGPGEWDRESRPSWVRLDRVIEVDQDGIRREGSILDRARFDQVAAQLRRDYGWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8T8</accession>
    <name>U5W8T8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family 1 extracellular solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561768.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35880"/>
    <dbReference type="EnsemblBacteria" id="AGZ45432">
        <property type="protein sequence ID" value="AGZ45432"/>
        <property type="gene ID" value="AFR_35880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7262"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_10_5_11"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666182" evidence="1">
        <location>
            <begin position="23"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="412" mass="43311" checksum="93AA47E72FB05835" modified="2014-01-22" version="1">MRRSRLVTAVAAALLLTTTACGSGFDDNGGSTTQQSSGPADLKIMIASSGDAETKAVNDATAKWAAASGNKATVTPAQDIVQQLGQALAGSTPPDVFYVDAARFADYASVGALEPYADKISNVDDFYPNLRQAFTYQDKLYCVPKDSSTLALIINTDLWSKAGLTDADIPTTWEQLTAAAQKLEAKNVVPLALGDTRDRIGAFLVQSGGWLVSDDGKQATADTPENVAALTYVQGLLKDGLAKYPSALDAGWAGEAFGKGKAAMTVEGNWIKGALSADFPDVKYTVKELPAGPKGKGTLSFTQCWGIAAKSKFKDQAIKFVEAMTTTEQQLSYAKAFGVMPSRQSAQTQYTSEFPADAPFVAGAAYGHGPVNAPKMDSVLADFDTGLQGLATAKPQEVLQRFQKNAQTALGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9B6</accession>
    <name>U5W9B6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Antibiotic transport system permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_36730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36730"/>
    <dbReference type="EnsemblBacteria" id="AGZ45602">
        <property type="protein sequence ID" value="AGZ45602"/>
        <property type="gene ID" value="AFR_36730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7430"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="275" mass="29245" checksum="7F3CB2BEB9FD651E" modified="2014-01-22" version="1">MRAGAARGVIELRQTCTNIADLWTYLFPAVLLVGTVFFMRDATVPGTDISLGARTLPSTFGMGLAFAGLVSTATLLVTDREDGTLLRAKATPNGMSAYLVGKIVLVGGMTLIGFVLQLVPSLFVVEGLHVDATGWLILMVLVPLGFVATMPLGAVIGSLFESPRNMGLVMLPVIGLVAISGIFYPISAMPGWLQGVAQVFPVYWLGLGMRSVFLPDALTVAELGHSWRTLETFGVLGLWAVAGLVVAPIVLRRMSRRESGSAVAARREKAMLRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9M8</accession>
    <name>U5W9M8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_37975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562184.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37975"/>
    <dbReference type="EnsemblBacteria" id="AGZ45849">
        <property type="protein sequence ID" value="AGZ45849"/>
        <property type="gene ID" value="AFR_37975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7680"/>
    <dbReference type="eggNOG" id="ENOG5032Z20">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186972_1_0_11"/>
    <dbReference type="OrthoDB" id="2052863at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046086">
        <property type="entry name" value="DUF6104"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19599">
        <property type="entry name" value="DUF6104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="59" mass="7122" checksum="30BEC29B619592A1" modified="2014-01-22" version="1">MYFTDRGIEELVERRGEESVTLEWVGDQLRRFVDANPEFEIPIERFATWLARDDEDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9V5</accession>
    <name>U5W9V5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561099.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32510"/>
    <dbReference type="EnsemblBacteria" id="AGZ44760">
        <property type="protein sequence ID" value="AGZ44760"/>
        <property type="gene ID" value="AFR_32510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6579"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_300852_0_0_11"/>
    <dbReference type="OrthoDB" id="761219at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666204" evidence="2">
        <location>
            <begin position="40"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="490"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="866"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="914"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="964"/>
            <end position="989"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1001" mass="102773" checksum="FC9783C8FA911CA5" modified="2014-01-22" version="1">MLALVLGAVRARLAQALTLLVLTGLIAAVAAAGPWYALAAGSRAADGDVASAPASQRTLSVRKIASTNGLPEPAVETFAGAVRGLLPLTGLQPVLGMTVGLAVDRGDARPSMSVSYRENFCDHVRLTGGSCPAAAYEGAISQDAAEQLGLRLGDSLKVQASQNTLPVTLKVVATYVVVDPAGSYWSNKLYRANGGIEPAFTVLDTFTDDQLTGPTITYDVEVPAELIRGDDGFDLGTRLSAADSAFTQADLRLVNPTWMLLATIARDRDTIRDGVVVALGQVLVLGLFAIGLAGRYTGRERRGDAALLKLRGSTRRGLLGLALGQHLVPMLAGALLGAPLGFLLARLLAGPVRGDDDRLLALGLAAAAVGATLLGGLLVLTVLEAAVLRIPVAGLIGRAASGRRDWRADVIDLVLLAVAVGATYQVRAGQSTGGLALVAPALVALAVALLFARLLGRLADRVGGAALHAGRLRLGLTAAQASRRPGIDRVFALVAVAVAIFVSTAGVWSAGREARVQRSEVELGATRVLSVQASNRTALEAAVRRADPGGKNAMAAVIDRESVPPVVAVDTSRLAAITRWRPEYGSLTTLAQAGDAQPLPPPAMVTGTGLTLRMTNDAKGPIRVSVVLQHEGTGIAHVVTFGPLKRGEHTVRRPVEGCTEAPGCRLVRWQLVGTDSESALPPVSAAVTLTGLSQQGPDGTVLDGAALGDSRRWRPDFRGAGVDVFAADGRLKLVMDRNEAGVPLVGNKVYAVDAALPLPIVLAGESPPAWQFSDPILYSFGGAAVRVRVAGTASVLPVVGRDGVLVDLETARRVAAEGDLGGTFQVWLAADAPDSVVDALRANGLTILGSDDVPERAALLGDQGTAVAATFALLAASVGLLLAAAAMAVAAAVERGRQAEQLLALRVQGMSLKNAVAIGYAGSGALVVAGLLTGLVAAAIARPAVGVQVRPFTDGWNLVPPPGALGAAALLLAGLVALLVLGVTGWLSVRPLVRTLRRSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAR2</accession>
    <name>U5WAR2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative two-component system response regulator PhoP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_40270</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562640.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40270"/>
    <dbReference type="EnsemblBacteria" id="AGZ46308">
        <property type="protein sequence ID" value="AGZ46308"/>
        <property type="gene ID" value="AFR_40270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8150"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_11"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="128"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="128"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="25166" checksum="024C847C253C6E4B" modified="2014-01-22" version="1">MARVLVVEDEESFSDALSYMLRKEGFEVSVAPTGTSALTQFDRTGADIVLLDLMLPEMSGTEVCRQLRQRSAVPIIMVTARDSEIDKVVGLEIGADDYVTKPYSPRELVARIRAVLRRQGAEAAEVTTPTLAAGPVRMDVERHVVTVDGSSVQLPLKEFELLELLLRNAGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRSKVEPEPSAPRYIVTVRGLGYKFEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBM3</accession>
    <name>U5WBM3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Barstar domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_35500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the barstar family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35500"/>
    <dbReference type="EnsemblBacteria" id="AGZ45356">
        <property type="protein sequence ID" value="AGZ45356"/>
        <property type="gene ID" value="AFR_35500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7185"/>
    <dbReference type="eggNOG" id="COG2732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059876_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000468">
        <property type="entry name" value="Barstar"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035905">
        <property type="entry name" value="Barstar-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01337">
        <property type="entry name" value="Barstar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52038">
        <property type="entry name" value="SSF52038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Barstar" evidence="2">
        <location>
            <begin position="167"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="27499" checksum="91B7284D581BA343" modified="2014-01-22" version="1">MGGPDAAIAVCADIDGLFTDSPLSVRETTLLGCLPHPPLSRALDALAKGAGNPGGALHRRAIDAFLYSVDRNGVADRLVDSQLRASVTGVRPSVLAAGLVDVTLDSAISETMPSSARTIWDLWHAGGPTESGLWAGCDRELRHHWLGAALAHHRADVRDKPAGRTYELDGRHITDVEGFYCAIGEAVNGPGGYFGWNGAALHACVTGRWGAEWPFRLIWRDAEVARTHLVAGAGPGGPATFDEILQWLAEDQIEVELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD17</accession>
    <name>U5WD17_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_37910</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562171.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37910"/>
    <dbReference type="EnsemblBacteria" id="AGZ45836">
        <property type="protein sequence ID" value="AGZ45836"/>
        <property type="gene ID" value="AFR_37910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7667"/>
    <dbReference type="eggNOG" id="COG3469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3979">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019399_1_1_11"/>
    <dbReference type="OrthoDB" id="1081028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003610">
        <property type="entry name" value="CBM_fam5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036573">
        <property type="entry name" value="CBM_sf_5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00495">
        <property type="entry name" value="ChtBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51055">
        <property type="entry name" value="SSF51055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666305" evidence="6">
        <location>
            <begin position="29"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="7">
        <location>
            <begin position="127"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="85"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="464" mass="48971" checksum="D44D4FB7042F2900" modified="2014-01-22" version="1">MRRSLILAVTAALAAAGSTVYIASSANAAAACAPAWSSSAVYVKDNQVSYQAKNYTAKWWTQNEVPTASGEWGVWADKGACGGTTPPPTTPPPTTTPPPTTPPPTTPPPTTPPPTTTPPPATGLPKHALIGYLHSSFANGSGYLKMADVPADWDIINLAFGEPTSATSGDIRFTLCPASECPGVESEADFINAIKAKRAAGKKVLLSIGGANGQVQLTTTAARDKFVTSVSAIIDKFGLDGVDVDFEGHSLSLNTGDTDFKNPTTPVIVNLIAALKSLKSRYGANFVLTMAPETFFVQLGYQYYGSGPWGGQDPRAGSYLPVIYAMRNDLTVLHVQDYNSGSIMGLDNQYHSMGGADFHIAMTDMMLAGFPVAGNTANMFPALREDQIAFGAPSSVSAGNGYVAPAGVQQAVTCLVKGQNCGGYTVRSGTNPNFRGLMTWSINWDKFYSWEFKNNHEPFLNGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDF4</accession>
    <name>U5WDF4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA polymerase factor sigma-70</fullName>
            <ecNumber evidence="9">2.7.7.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_38635</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562315.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38635"/>
    <dbReference type="EnsemblBacteria" id="AGZ45981">
        <property type="protein sequence ID" value="AGZ45981"/>
        <property type="gene ID" value="AFR_38635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7817"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043648_0_0_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014305">
        <property type="entry name" value="RNA_pol_sigma-G_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02960">
        <property type="entry name" value="SigX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="21"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="129"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="SnoaL-like" evidence="8">
        <location>
            <begin position="199"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="313" mass="34345" checksum="590D7E2FCFD3194D" modified="2014-01-22" version="1">MEEATLLTAAQSGDADAFGRLVGSYRDELLAHCYRMLGSVDDAEDALQESLVRAWRGLGTFRAGSIRPWLYRIATNRCLTLIEQRARRELPADLSPGAPLNEVAWLEPYPDRRLGPAATVEQREGIGLAFVAALQHLSGLQRAVLLLREVLGFSAAEVAGQLDTTVAAVNSGLQRARAVLAQRRPPVTPTDDKIKELADRYASAWQAADVDTIVAMLTDDATYSMPPLPEWYSGRAAIRDFLLSGPLTHRWRFVPAAANGQLAFGTYLWDERRAAYAWAGLDVLRLDGDRIAEVVSFLTPGLQGDFGLPDEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE51</accession>
    <name>U5WE51_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40255"/>
    <dbReference type="EnsemblBacteria" id="AGZ46305">
        <property type="protein sequence ID" value="AGZ46305"/>
        <property type="gene ID" value="AFR_40255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8147"/>
    <dbReference type="HOGENOM" id="CLU_2140470_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666295" evidence="1">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="112" mass="11758" checksum="D15E88E54CE5CF94" modified="2014-01-22" version="1">MLRSRWFALGLAVLMTGCSDAGPPEAGIPSPSASARPSAAELCAYLESRLPRLQELDSKASAQGNLTGGLYGWYEQRGAAPDPAELDRLTRSACPGVRDQVLEATGMSSFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEA9</accession>
    <name>U5WEA9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sec-independent protein translocase protein TatB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562599.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40060"/>
    <dbReference type="EnsemblBacteria" id="AGZ46266">
        <property type="protein sequence ID" value="AGZ46266"/>
        <property type="gene ID" value="AFR_40060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8107"/>
    <dbReference type="eggNOG" id="COG1826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_2_1_11"/>
    <dbReference type="OrthoDB" id="2073616at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="146" mass="16042" checksum="52B70C19A169A193" modified="2014-01-22" version="1">MFENLNWWEIGALLMLALLIFGERLPKVIGDGLRMLRGLRAMAQNATTDLSRELGTDIQLQDLHPKAFIRKHLLSEEDEQAIRKPLQGLFEDVKSDLDGVKSDLSDVASAADLKARRNDISPASNGSGAAVEKSTPAPRTFDLDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WES2</accession>
    <name>U5WES2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562778.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40960"/>
    <dbReference type="EnsemblBacteria" id="AGZ46446">
        <property type="protein sequence ID" value="AGZ46446"/>
        <property type="gene ID" value="AFR_40960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8292"/>
    <dbReference type="eggNOG" id="ENOG5033I6R">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168298_0_1_11"/>
    <dbReference type="OrthoDB" id="1910692at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022536">
        <property type="entry name" value="EspC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10824">
        <property type="entry name" value="T7SS_ESX_EspC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="106" mass="11378" checksum="95AF9F53B9A7FAC4" modified="2014-01-22" version="1">MADGFQVDAAQIRRHIAGLEAVRSRFGAVKSASAAISQDDGAYGLLCSWLPAVLEGRHRRQDELLAYVEENLTLAADSLTAVAGDYEDTDNAAADAVRKAGMRLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFH7</accession>
    <name>U5WFH7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_42680</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42680"/>
    <dbReference type="EnsemblBacteria" id="AGZ46790">
        <property type="protein sequence ID" value="AGZ46790"/>
        <property type="gene ID" value="AFR_42680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8638"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005571_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="500"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="8">
        <location>
            <begin position="735"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="521"/>
            <end position="528"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1053" mass="115662" checksum="C3A12DB7350DE64C" modified="2014-01-22" version="1">MPSDSAGSSVTADTYVADLHIHSKYSRACSRDLTLPNLAWWARRKGIAVLGTGDFTHPAWFDHLRENLHPADEPGLYRLSGDAEQEVSRKLPPILQGTDNARFMLSVEISTIYKRDDRTRKVHHLIYMPDLDAAARFNTALGRIGNLTADGRPILGLDSRDLLEIVLEASPDGYLVPAHIWTPWFSALGSKSGFDAIADCYADLADHITAVETGLSSDPEMNWRVSSLDRYRLVSNSDAHSPAALAREATLFTGTPDYFAIKDGHTLAGTLEFFPEEGKYHADGHRVCGINWAPQKTRDHGGRCPECGKPLTVGVLSRVEDLADRTTGGERPQDVVHLIQLAEILGEINGVGPKSKTVDGQLNHLVAGLGAELDILRRVPLDDIGQTGGEQLREAIDRLRRGDVRRISGYDGEYGVITLFEPGELRNKQSAPQAETLFDMLPVPVPQQRKAEPAIGRATVPKAVKEPKKKAPPLPPPASPHEPFEPMLAGMEEVGTGLLDRLDAMQRVAASAPGGPLLIVAGPGTGKTRTLTHRIAYLCAELGVFPERCLAITFTRRAAEEMRHRLDALMGDVAEDITVGTFHSVGLTILKENAKAAGLGAHWRIADDAERTAAREQAGDDDHAYAKLLRQQDLVDLDDLLTMPVALLRDEPDLVERYRKRWQWIFVDEYQDVDDVQYELLRLLSPATGNLCAIGDPDQAIYSFRGADVGYFLRFSRDFTDARLVRLTRNYRSSAPILAAAVQAIAPTTLVAGRRLDPARVNPESPLVGRYFAGSVADEADFVVRTIDELVGGLSHRSLDSGRIDSRAVTPGTLSFADIAVLYRTDAQAQAVVDALSRAGVPVQKRSHNRLRDRPGVQIIAHELQHADGLGGSLAARVKLTAQVLAQRYAAPTLDAEQLAPEDIWTAADLLMPLAHRIGEDMTRFLQEIATGAEVDALDPRAEAVNLLTLHAAKGLEFPVVFLVGCEDGLLPLRWPGTKPTDDETAEERRLFFVGLTRAQDRLYVSHAARRFRHGKEREQVPSPFLDAIDTGLFEKMGDRVPSRPKDRQLRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP43</accession>
    <name>U5VP43_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357676.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02220"/>
    <dbReference type="EnsemblBacteria" id="AGZ38733">
        <property type="protein sequence ID" value="AGZ38733"/>
        <property type="gene ID" value="AFR_02220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.449"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_7_3_11"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="23079" checksum="BE319FDD65ECD08F" modified="2014-01-22" version="1">MEFDEVLRRRRMIRTYDPDRPVPPELVDKLIKHALRAPSAGFSQGWGFLVLQEQADRDLYWSVTAPADEPAAGSWLDRMRTAPLIIVALSNKSVYLDRYAQADKGWTDRDESRWPVPYWDIDTGFAALLIHLTAVNEELASLFFGVPAERVGPFREAFGVPEEFVPVGALTVGYPAADKRSPSLRRGHRPVDEVVHHGRWAGSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPF7</accession>
    <name>U5VPF7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357796.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02820"/>
    <dbReference type="EnsemblBacteria" id="AGZ38853">
        <property type="protein sequence ID" value="AGZ38853"/>
        <property type="gene ID" value="AFR_02820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.566"/>
    <dbReference type="eggNOG" id="ENOG5033RSJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873277_0_0_11"/>
    <dbReference type="OrthoDB" id="894977at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="36424" checksum="09812B3AB51AF213" modified="2014-01-22" version="1">MIHRPLLGADPEDNRRVLQQVLDTVPAGKLELPPGVHVLADGLRVPGGWTIRGSEIAGANGGPPGTWLESIGTTGHPVLHIVGSDVAVSDLGLRPPPADPGEHGGDRGTAVTVGEYLYPATPEWIARVDLRRLHVERPTEKHANCIALMGAVRDVTLHDITVRGGYTGVAVHWGAVGADVSSIEGPTYHPHHLTITDLRVSDAIEGFYLSSVHDVRVEGACLRDVEMGFRLLPGDNTDRFVSSPVVGSAITIADVCVRWHGPRYAVRVAGWGRSEVDGVVSVLRYRDTVIRDCVLRGSGSAESWSPMVVERADGVELRDNVFETSTSSDSSDCCIRTPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPR7</accession>
    <name>U5VPR7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_03320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357896.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03320"/>
    <dbReference type="EnsemblBacteria" id="AGZ38953">
        <property type="protein sequence ID" value="AGZ38953"/>
        <property type="gene ID" value="AFR_03320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.668"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="297" mass="32764" checksum="F77BFC38D33B905C" modified="2014-01-22" version="1">MTEAISVAGLTKNFGRTRALTGLDLSIRTGEVHGFLGPNGAGKTTTIRILLGLMRPDGGTASLLGGDPWADATELHKRLAYVPGDVTLWPTLSGGEVIDLLGRLRGGLDPRRRDELLERFALDPRKKGRTYSKGNRQKVALVAALASDVELLILDEPTSGLDPLMEEVFREVIREERRRDRTVLLSSHILSEVEALCDRITIVREGRTVETGTLTDMRHLMRTSIEAELTGPVDGLATLPGVHDLRVEDHRVRFDVDTDSLEPALQKLVGAGVRSLVSQPPSLEELFLRQYRQEPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPV3</accession>
    <name>U5VPV3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03495"/>
    <dbReference type="EnsemblBacteria" id="AGZ38988">
        <property type="protein sequence ID" value="AGZ38988"/>
        <property type="gene ID" value="AFR_03495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.702"/>
    <dbReference type="eggNOG" id="COG0431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="7"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="191" mass="20949" checksum="90C9DA45A545E21F" modified="2014-01-22" version="1">MIMPNLQVIVASTRPGRVGLPVATWAAAAAVEHGVFDVELIDLADYALPLMDEPNHPRLRQYTHEHTLRWSETVDRADAFVIVHPEYNYSFNAAIKNAIDYLHQEWADKPVGFVSYGGVSGGLRATQALKQVVTTLKMVPIVEAVPIPLVGRYLNDDGEFVPSEQIAAAAAPMFTELARLHGALAPLRARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQD1</accession>
    <name>U5VQD1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358083.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04245"/>
    <dbReference type="EnsemblBacteria" id="AGZ39138">
        <property type="protein sequence ID" value="AGZ39138"/>
        <property type="gene ID" value="AFR_04245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.857"/>
    <dbReference type="eggNOG" id="ENOG5033ZY9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850854_0_0_11"/>
    <dbReference type="OrthoDB" id="1847009at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665963" evidence="1">
        <location>
            <begin position="33"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="14097" checksum="C540AD6C968C20CF" modified="2014-01-22" version="1">MTRIAPRAATTAALATAGLAGLLGAAAQPAAAAPSALATPCVQKIAVVNNGGFVMNFQVTTRDGQLSAPTDDYPVNQWRLVDLTSTPLAEGVDVRPLVHATAGDDVLGNNFVSYCTNGQTATYTASGTTLNYTVTLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQI8</accession>
    <name>U5VQI8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03950"/>
    <dbReference type="EnsemblBacteria" id="AGZ39079">
        <property type="protein sequence ID" value="AGZ39079"/>
        <property type="gene ID" value="AFR_03950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.798"/>
    <dbReference type="HOGENOM" id="CLU_2340491_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="9875" checksum="3D93F16E83E6E714" modified="2014-01-22" version="1">MPTRSATIVIAMTDMTISRSNVSFRGPAWRRRTTGVVLAATLAVAAAFGPGHVLATRLGAGELVGFSTVSAADTITSSDVLAEVPAAGRGRSVSESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQK7</accession>
    <name>U5VQK7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358012.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03890"/>
    <dbReference type="EnsemblBacteria" id="AGZ39067">
        <property type="protein sequence ID" value="AGZ39067"/>
        <property type="gene ID" value="AFR_03890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.786"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_4_11"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="203" mass="22190" checksum="D71E16746B615D51" modified="2014-01-22" version="1">MTTSGRLPYPIALTGDGIQLREWRDEDLEELVHLLDEPDIARWTPMPSPFDPEAGVAYLKRARQSRANGQRIQLAITAFGDKPVGEVLLFGVDAGLREAELGYLVGASYRRRGFASTSLSLLSGYARSTLGLSRLLLRIDPANVASCAVARRCGYQLTGEPPILQEGPYGSSSLDTWELVSAGGGTRLERLRNVAARRYGRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR04</accession>
    <name>U5VR04_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="4">IMP dehydrogenase</shortName>
            <shortName evidence="4">IMPD</shortName>
            <shortName evidence="4">IMPDH</shortName>
            <ecNumber evidence="4">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">guaB</name>
        <name evidence="11" type="ORF">AFR_04950</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 9">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358338.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04950"/>
    <dbReference type="EnsemblBacteria" id="AGZ39279">
        <property type="protein sequence ID" value="AGZ39279"/>
        <property type="gene ID" value="AFR_04950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1000"/>
    <dbReference type="eggNOG" id="COG0516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_11"/>
    <dbReference type="OrthoDB" id="532857at2"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="97"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="157"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="4">
        <location>
            <begin position="340"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="4">
        <location>
            <begin position="363"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="4">
        <location>
            <begin position="387"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="4 5">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4 5">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="251"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <begin position="300"/>
            <end position="302"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ39279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="495" mass="52577" checksum="D9713E561004F5CD" modified="2014-01-22" version="1">MDNESARTVPLGLTFDDVLLQPGESDVVPSRVNTITRLTRNVELSIPLLSSAMDTVTEARMAIAMARQGGIGVLHRNLSLEDQASQVDLVKRSESGMITNPVTCSPDDTLREVDQLCGRYRISGLPVVDADGVLVGIVTNRDMRFVTDNSIKVRDVMTKAPLISAPVGVSKDEALALLQRHKVEKLPLLDDAGRLRGLITVKDFTKSEQYPNSAKDAQGRLRVAAAVGVGDDAYKRARLLIDAGVDVVIVDTAHGHQRAVLEMVARLKKDTTTDIVGGNIATYAGAKALVEAGADAVKVGVGPGAICTTRIVAGVGVPQITAVMEASRACRPAGVPVIADGGIQYSGDIAKAIVAGADTVMLGGLLAGSEESPGDLIFINGKQYKTYRGMGSLGAMQSRGQAKSYSKDRYFQQDVTEDKLVPEGVEGQVPYRGPLSRVAHQLIGGLRAAMGYVGAETIPDLQEKGQLIRITAAGLKESHPHDIQMTVEAPNYHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRX6</accession>
    <name>U5VRX6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_06475</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359115.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06475"/>
    <dbReference type="EnsemblBacteria" id="AGZ39584">
        <property type="protein sequence ID" value="AGZ39584"/>
        <property type="gene ID" value="AFR_06475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1313"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_1_11"/>
    <dbReference type="OrthoDB" id="1996723at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="5"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="3">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="16900" checksum="2702F5CF369D1C8D" modified="2014-01-22" version="1">MTERLTPGDPAPEFTLPTDNGDTLTLEDLRGRKVILYVYPAAMTPGCTTQACDFRDSLGSLQAAGFEVVGISPDVPAKLAKFRERDAITFPLVSDQDKSVLTAYGAFGEKQLYGKTVTGVIRSTFVIDENGTIEKALYNVKATGHVAKLRRDLGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSN0</accession>
    <name>U5VSN0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Integral membrane transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02155</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02155"/>
    <dbReference type="EnsemblBacteria" id="AGZ38720">
        <property type="protein sequence ID" value="AGZ38720"/>
        <property type="gene ID" value="AFR_02155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.436"/>
    <dbReference type="eggNOG" id="COG2807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038046_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="38978" checksum="3EE9DB602F47D392" modified="2014-01-22" version="1">MGLVLAAINLRPAVTSLGPVLEEVRASLGMSAAVAGLLTSVPALCFALVGFTAPLLARRWGTGGVIALGMTALAAGLTVRPFAGGTASFIALTALALAGIALANVLLPVVVKQRFPDRVGAMTGLYSVALNLGASTAAAVTVPLTGAFGGDWRWGIGVWALVAASALPAWVLLARERTPSTTPSVPVTAAPALRLTRNPIAWSLAVYFGLQSTVAYVIMGWLPQIFRDAGISAGTAGLLFAVTALLGVPLSFVLSAVAGRLPRQSGVAVIIGLFGLAGYAGLWADPAAAPWLWVVLLGVANCSFPLALTMIALRGRDTATVVRLSAFAQSTGYLLSIPGPIAVGILYEHTGSWKIPLAVLALLMVPQMIAGFLAGRDRQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSN8</accession>
    <name>U5VSN8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTPase Obg</fullName>
            <ecNumber evidence="7">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GTP-binding protein Obg</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">obgE</name>
        <name evidence="7" type="synonym">obg</name>
        <name evidence="12" type="ORF">AFR_07690</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359358.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07690"/>
    <dbReference type="EnsemblBacteria" id="AGZ39827">
        <property type="protein sequence ID" value="AGZ39827"/>
        <property type="gene ID" value="AFR_07690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1568"/>
    <dbReference type="eggNOG" id="COG0536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011747_0_1_11"/>
    <dbReference type="OrthoDB" id="603226at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036346">
        <property type="entry name" value="GTP-bd_prot_GTP1/OBG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015349">
        <property type="entry name" value="OCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="PTHR11702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09269">
        <property type="entry name" value="DUF1967"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102741">
        <property type="entry name" value="SSF102741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="SSF82051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03595">
        <property type="entry name" value="Obg_CgtA_exten"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51881">
        <property type="entry name" value="OCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="11">
        <location>
            <begin position="2"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="9">
        <location>
            <begin position="160"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="OCT" evidence="10">
        <location>
            <begin position="348"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="481"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="166"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="191"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="212"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="282"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="311"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ39827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="504" mass="53318" checksum="FCFB6D5F363D00B5" modified="2014-01-22" version="1">MATFVDRVVLHLQAGDGGHGCASVRREKFKPLGGPDGGNGGHGGGITLVVDPQQHTLLDFHFRPHVKAQNGTGGAGANRDGANGGDLVLSVPDGTVVQTPDGEVIADLIGAGTTFEVARGGRGGRGNASLANARRKVPGFAELGEPGDALDVVLELKSVADVGLVGFPSAGKSSLISVMSAAKPKIADYPFTTLVPNLGVVQAGDHTFTVADVPGLIPGAATGKGLGLEFLRHIERTSVLVHVLDAAAPEIERDPLADLDAIEAELAAYGGLEERPRLVVLNKIDIPDGKDLAEMVRPELEARGLPVFEVSAITREGLKELMYALGDAVAANRLAAPAPESTRTVVRPRAVDEKGFTIERDVDGVFVVRGPQVERWVRQTNFDNDEAIGYLGDRLDRLGVEAALAKKGAEAGDPVRIGDREFDWQPNTGEYISGPRGTDIRLEEVDTRPSAASRLAARKARRVRGDDELLHMAEDGTVSTISMASRPVLIEDDHDEDEDDTDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTB8</accession>
    <name>U5VTB8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major cell-surface adhesin PAc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359900.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09695"/>
    <dbReference type="EnsemblBacteria" id="AGZ40228">
        <property type="protein sequence ID" value="AGZ40228"/>
        <property type="gene ID" value="AFR_09695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1976"/>
    <dbReference type="eggNOG" id="ENOG50328EM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1280934_0_0_11"/>
    <dbReference type="OrthoDB" id="2229747at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="26651" checksum="D3E3B28EFF71C253" modified="2014-01-22" version="1">MTLWRRTRTEVAGAVRSVRYDMGRREADEPGPSPLSGVTAFPDVTSTGMSTFGGAGVTGGLRTSYGDEFMPRPRRLAAVAAFGVLAVAGAAGSYFAVVNGIGSLAGEKPAGAEPYPLAAEAPHTGELSNSGFGRGTAAVPDEGRTETAPAQIRVVPTTAAGAAAAAVEPGVTQARPQQTTVERTTPATRPTSCCLNPPVPTPTVPVPTPHSPSVTPSPTASSPETTSPTPTETVDPVTPSASDEDEDTGEGENHARRARRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTC1</accession>
    <name>U5VTC1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LinF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08975"/>
    <dbReference type="EnsemblBacteria" id="AGZ40084">
        <property type="protein sequence ID" value="AGZ40084"/>
        <property type="gene ID" value="AFR_08975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1826"/>
    <dbReference type="eggNOG" id="COG1708">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086371_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="179" mass="20020" checksum="AF850AA10066B60A" modified="2014-01-22" version="1">MREQLRLIDRVRSACRADTDLDAALMYGSFVQGSADSHSDIEFWLFFTAPPSQGPRAWIERIAPVRYVVLNEFGSHVAFFPGLIRGEFHFAGVADIASVRDWPASPVEIIVDRHGLLGAARPAGEIDMTADVCGRFANWLLLAHHVGRRGELLRQRDALAHAQRHLLWMARLAEGRTGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTD7</accession>
    <name>U5VTD7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09820"/>
    <dbReference type="EnsemblBacteria" id="AGZ40253">
        <property type="protein sequence ID" value="AGZ40253"/>
        <property type="gene ID" value="AFR_09820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2002"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="15572" checksum="9E71C3C2F19422AE" modified="2014-01-22" version="1">MVAVKESLREARNQLSVFSRQVGTRLELKDADLDCLDLIDRAGPLSPTALARLTGLHPATLTGILDRLDRGGWVVRERAENDRRAVLIRSVGDRKGELYALLAGMNRGIDEICAGYSPAELEVIADFLRRTAETGRQATGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTE8</accession>
    <name>U5VTE8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359684.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40119">
        <property type="protein sequence ID" value="AGZ40119"/>
        <property type="gene ID" value="AFR_09150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09150"/>
    <dbReference type="HOGENOM" id="CLU_1182967_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="26800" checksum="EB3E75FEE53783EB" modified="2014-01-22" version="1">MTLLAPAAQQQSLWQLLLIAGAGSLIGGLLSLLGVVLTQRGNRKKDAADRVARWKREDEVKAIEERRRLLLDIAEAVQERQSWIRQVDYGYEHDPSGLDVPRFQVAGRVELFATVPVMWLWDDFNDRLLAVQAEANAGNHHVDVHTGRNELDDQTLAPRAKVAGDILTLLLRRMLRTDKEIVAGEDFLSRVSLLGSLFDYDERHKKAVEEYLKQMKHPETPWQKEWLDWSSSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTL4</accession>
    <name>U5VTL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Lipid A export ATP-binding/permease protein msbA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_10245</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10245"/>
    <dbReference type="EnsemblBacteria" id="AGZ40338">
        <property type="protein sequence ID" value="AGZ40338"/>
        <property type="gene ID" value="AFR_10245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2087"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="22"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="341"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="374"/>
            <end position="381"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="590" mass="62365" checksum="C9E51E1CE3DBDBBC" modified="2014-01-22" version="1">MLGRGLRVLGSAIRTEPRLFTIGTVGSSLFGLLVIANSYVVGYVIGHVVVPAFAENRAGAGELGLIAGAFIGIALLRVASIFGRRLGAGYMQFRLQARYRSAVTRRYLALPPAWHQRHATGTLLSNANSDVEAAFVPIAPLPFAVGTLVMIAAAIVSLFFTDWVLALVGVCLFPALFLLNLVYSRRMSPRQIRAQQLRGKVSAVAHESFDGALVVKTMGREADESRRFAVHVTELRDALISVGRLRGLFDPLMDSLPSVGTLAVLLLGAYRLQSGAVSVAELVSVAFLFTVLAFPVRAIGWVVAELPRSVAGWERVQAVLGAEGDLAYGTSALERTGPAALTFDRVTFSYATGGDVLHDVSFTVPAGKTVALVGATGAGKSTIASLAVRLVDPDSGSVRADGTPLPELSVPALAETTALVPQIPFVFDDTVRGNVTLDRDGLDDEAAWTALRLAQAEGFVEKLPDGLDTAVGERGTSLSGGQRQRLTLARALAGRPRLLVLDDATSAVDPRVEAAILASLRGHDSGASILVVAYRRATIALADEVVFLENGRVVATGTHHDLLATQPGYLDLVTAYEQAEAAREDESVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTQ0</accession>
    <name>U5VTQ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Leucine-responsive regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359799.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09440"/>
    <dbReference type="EnsemblBacteria" id="AGZ40177">
        <property type="protein sequence ID" value="AGZ40177"/>
        <property type="gene ID" value="AFR_09440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1924"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170329_3_0_11"/>
    <dbReference type="OrthoDB" id="1855714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AsnC_trans_reg" evidence="1">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="95" mass="10017" checksum="15E05FBA823C2AC9" modified="2014-01-22" version="1">MNTAIVHIDCATDSIPEVAEALAALPGVSEVYSVAGNVDLIAIVRVPQFEDIAEVIAGRISKTPGVLNTESHIAFRAYSKHDLEDAFAIGLPDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTQ2</accession>
    <name>U5VTQ2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hva1_TUDOR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360195.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10445"/>
    <dbReference type="EnsemblBacteria" id="AGZ40378">
        <property type="protein sequence ID" value="AGZ40378"/>
        <property type="gene ID" value="AFR_10445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2128"/>
    <dbReference type="eggNOG" id="ENOG50336GE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180079_1_1_11"/>
    <dbReference type="OrthoDB" id="2028293at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021331">
        <property type="entry name" value="Hva1_TUDOR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11160">
        <property type="entry name" value="Hva1_TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hva1_TUDOR" evidence="2">
        <location>
            <begin position="10"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="72" mass="7825" checksum="F2950C0074EE43C7" modified="2014-01-22" version="1">MADDENLSKGEKVTWNSHGQKVHGTVEKKITKSTSEAGRKVAASEDDPQYKVKSAKTGREAVHKPGALHHDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU39</accession>
    <name>U5VU39_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_10190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360096.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10190"/>
    <dbReference type="EnsemblBacteria" id="AGZ40327">
        <property type="protein sequence ID" value="AGZ40327"/>
        <property type="gene ID" value="AFR_10190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2076"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_22_1_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="9"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="105"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="29926" checksum="9FBD211BF014A15E" modified="2014-01-22" version="1">MDQLARRFESDRPHLRAVAQRMLGSAAEADDAVQEAWLRLSRSDTSDVGNLTGWLTTVVSRVCLDMLRSRASRREEDAELPETAAPDDAHPEHEALVADSIGPALLVVLDTLAPGERLAFVLHDMFAVPFPEIAEIVGCSPAAARQLASRARRKVQGRGANSGSDRTRTRTVVEAFLAASRGGDFAALLALLDPDVVLHADDAAVRTGATAVLRGASAVAETFSGRAQGAKLAFLDGTAGLLWAPGGTPRVAFAFTVAADGRITGIELIADAADLAAMDVQRVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU51</accession>
    <name>U5VU51_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_04310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04310"/>
    <dbReference type="EnsemblBacteria" id="AGZ39151">
        <property type="protein sequence ID" value="AGZ39151"/>
        <property type="gene ID" value="AFR_04310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.870"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_2_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="176" mass="19495" checksum="766B8767DA5F24A6" modified="2014-01-22" version="1">MHSTALRLMTERGFDAVSIDDIVAAAGISRRTFFNYFADKESVVFDPDPDDAALWAALLDERPPDEPLWDSLRELLIGYTTAAAERMLLQRRVRLASPELSGCSREVADRFWDVVREWAATRAGLDALRLDLTVNAARTVLNTACPHWDATSGITGLHAHLAAGFDFVHPAERISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU67</accession>
    <name>U5VU67_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="14">4.2.1.136</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ADP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nnrD</name>
        <name evidence="17" type="ORF">AFR_04830</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 15">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 14 15">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 14 15">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="15">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 15">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="14"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04830"/>
    <dbReference type="EnsemblBacteria" id="AGZ39255">
        <property type="protein sequence ID" value="AGZ39255"/>
        <property type="gene ID" value="AFR_04830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.975"/>
    <dbReference type="eggNOG" id="COG0062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NADHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052857">
        <property type="term" value="F:NADPHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="SSF64153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12 14 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14 15" id="KW-0520">NAD</keyword>
    <keyword evidence="9 14 15" id="KW-0521">NADP</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="378"/>
            <end position="382"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ39255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="465" mass="46068" checksum="B8CAE5A0F3F70790" modified="2014-01-22" version="1">MATLPPGTLMQRAAAGLARRCALLLRESGGVYGSTVVLLVGSGDNGGDALYAGATLAARGAAVKAILLSPERVHLAGLAALRRSGGVTVAGLPSRADLVVDGIVGIGASGGLREPAAALVKSLSDLRGRDGARPPVVAVDVPSGVAVDTGDVPGDAVQADVTVTFGCLKPAHVVGAAAALAGHVELVDIGLGATLHADPAVRVPDAADIAEWWPRTGPGSDKYSRGVVGVATGSATYPGAAILSVAGALAGPTGMVRYAGSADSEVVQHHPSVVAAKRVADAGRVQAWVCGSGLGTDDGARTELRSVLATSLPVLLDADALTLLVDGQHATDLRRDAPLVITPHDGEFARLALERPGADRIGAACKLAAWINAVVLLKGDRTIVATPAGEVWVNPTGSSALATAGSGDVLAGLLGSLLAAGLPPERAAIMAAYAHGLAGRLAAEGGPVTAPDVAAALRPVLADLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU69</accession>
    <name>U5VU69_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glutamate synthase (NADPH/NADH) small chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_10340</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360155.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10340"/>
    <dbReference type="EnsemblBacteria" id="AGZ40357">
        <property type="protein sequence ID" value="AGZ40357"/>
        <property type="gene ID" value="AFR_10340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2106"/>
    <dbReference type="eggNOG" id="COG0493">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_3_1_11"/>
    <dbReference type="OrthoDB" id="671513at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="36"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="491" mass="52512" checksum="55041D2430C03121" modified="2014-01-22" version="1">MPDPNGFLRYERQLPKRRPVPVRIQDWREVYPPAGEELIRDQATRCMDCGIPFCHEGCPLGNRIPDWNDLVRTNAWSAAAESLHATNNFPEFTGRLCPAPCEAACVLGIADDPVTIKQVEVEIANHAFAAGLVPQPPVTLSGRSVAVVGSGPAGLAAAQQLARAGHSVTVYERDDQIGGLLRYGIPDFKLEKQRIDERLAQMAGEGVIFETGVNVGVDVTADDLRERFDAVLLTCGALAGRDTAGTPGRQLNGVHLAMEHLVPANRVAAGLQDTTPIDAQGKHVVIIGGGDTGADCLGVAHRQGAAGVIQLDQYPLPPEVRAGELHPWPTWPVILRNYPAHEEGGERVFGVAVQEFVDDGNGNVVSVRVADVMVERVDGRRTVTVQEGSERDLRADLVLLAIGFEGTEQQPLLTQFGVTRNGRGAVDADDNWQTDAEGVFVAGDMHRGASLIVWAIAEGRAAAAAIHTYLGGFGELPAPVRPSSQPLAAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUJ2</accession>
    <name>U5VUJ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_10990</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10990"/>
    <dbReference type="EnsemblBacteria" id="AGZ40487">
        <property type="protein sequence ID" value="AGZ40487"/>
        <property type="gene ID" value="AFR_10990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2238"/>
    <dbReference type="eggNOG" id="COG3428">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024617_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="DUF304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_2" evidence="3">
        <location>
            <begin position="64"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_2" evidence="3">
        <location>
            <begin position="246"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_2" evidence="3">
        <location>
            <begin position="355"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="612" mass="63637" checksum="E16761D4E2E52C26" modified="2014-01-22" version="1">MAVAEGEPRRRLHPLSPLLSGAKSIAVIVAALSWQTLSQVGVERFALVVGVIAVGVVIFSVIGWLNTGYHVVGRELRIQDGLLWRRNRAIPLDRLQAVELRRPLLAQLTGLAELRLEVVGGGKTEAPLAYLTVREAAALRERLLALAGRTPGAVDRAATDPAAGAPALERPLFRVGNGDLVVSQLLTPQAFLLPVGVAFVIMQFVLEGSWTFVGIASTVTAMAGVLLQPVRRVLQDWDFRLARDPDGRLAVRYGLLETRSQIVPLNRVQSIGVTWPLLWRAKGWLHLRLDIAGYAGPQSNNDKRSDRLLPVGSPEVARALVWEVLPGVDLAALATSPPPSRARWLNPIALKSMGVGLTDEVFVSRSGVLTRELQTVPYARLQSVRVIQGPLQRLLGLATVYGDTAGGRSGVARDRDVAEAWWLADQLSLRARHARGPAPLVTATVADPAEVAADPAAPQALPIDDRAAEQWAAPGASADSAWPNPSEPGTAAPGQATLGEAASDRATPGHTASGHTASGHTASGQASSGHAAPGQVALGHATTGQAAPSQAPAGQAPAGQAPAGQAPAGQAAAGAGATGPSATGRGASGSGGEVPGEGASEDDSFWRRPAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUL4</accession>
    <name>U5VUL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein piccolo</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05655"/>
    <dbReference type="EnsemblBacteria" id="AGZ39420">
        <property type="protein sequence ID" value="AGZ39420"/>
        <property type="gene ID" value="AFR_05655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1147"/>
    <dbReference type="eggNOG" id="COG3667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893751_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="32582" checksum="05F14A5DD94DEB82" modified="2014-01-22" version="1">MIRPLRDLAAYALVAAPAVWLFVAVIRLIPSGVGQDFLTRVQNSFYSFVNVELVALPIAAVLLATLVQPVHKHAKLITVAALIEYAVAGFFAIVFGFLIGLINIARWSVRVAFEEFLVRTAWLAVFAAAAYAVFLIWRNLYYTPKPKAPQGMYGQPQQQWQQQPPPQYGGQPGYLQPGQPGQPGQPGFGQQPPPGQWGQPQQPYAQPAWGEPTTVQPPVAPPSSAPPAQPVSAQPASGGAPGTPPGPFAPQPGHPQGYGAPSYPPQPPYGQPQPGPADATQAMPPRPADEDRTQIVGEEHPPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUR2</accession>
    <name>U5VUR2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Winged helix family two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_11450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11450"/>
    <dbReference type="EnsemblBacteria" id="AGZ40579">
        <property type="protein sequence ID" value="AGZ40579"/>
        <property type="gene ID" value="AFR_11450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2334"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="121"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="121"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23559" checksum="D717F0DA6221351A" modified="2014-01-22" version="1">MLVVEDEDAIRAPLAAALRAAGHEVRECADGAGFEDVVDGFRPDLALLDVQLPGGRDGFALAGVLRNRSDCAVLMVTARDAMDDRLRGFAAGADDYVVKPFAIVEVLARVSAILRRLGRVPSAVQVGDLVVDEDARVAVRAEAALDLTATEFKLLAYLAGHRGRILSKTQLLTQVWGYDEYDPNLVEVHVSALRRKLEQHGPRLIHTARGLGYVLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV00</accession>
    <name>U5VV00_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359012.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05960"/>
    <dbReference type="EnsemblBacteria" id="AGZ39481">
        <property type="protein sequence ID" value="AGZ39481"/>
        <property type="gene ID" value="AFR_05960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1211"/>
    <dbReference type="eggNOG" id="COG3167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116140_0_0_11"/>
    <dbReference type="OrthoDB" id="1914740at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043683">
        <property type="term" value="P:type IV pilus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043107">
        <property type="term" value="P:type IV pilus-dependent motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007445">
        <property type="entry name" value="PilO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04350">
        <property type="entry name" value="PilO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665748" evidence="2">
        <location>
            <begin position="38"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="21495" checksum="27F6CE0BCB1F94E4" modified="2014-01-22" version="1">MGARHADRLWMFAGALVILLLAAVSWFVLISPQYAEAEEIRSQTGTSQDQIVGLNKTIAQLKKDQAKLKALRATLATNQEALPEDSGVPDFLRQLQSSGDKINVNVSGVTVSTPVQVEGTARVWALPITLTADGTPANLGLFLDELQSGQARAVLIDSANLAPNTTESDADSGGNEPESMSLNLTVKAFVAPPAGAGAPTVTTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVE6</accession>
    <name>U5VVE6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_13460</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13460"/>
    <dbReference type="EnsemblBacteria" id="AGZ40978">
        <property type="protein sequence ID" value="AGZ40978"/>
        <property type="gene ID" value="AFR_13460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2733"/>
    <dbReference type="eggNOG" id="COG2807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038046_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="12"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="396" mass="40417" checksum="F0A2D3AF96E8FB15" modified="2014-01-22" version="1">MSTPTTIAPARLARGILIAVALTALNLRTAVTGFSVLTGDAAEDLHFGPLVAGAIGTIVTGCFAVAAFAAPPLARRLGLERTAALAVTATTVGILLRAVAWSPAVMIVATVIAFAGVGASNVILIPIVKQHFPERLHAVSTMYMVLLQVGQLAAPLLALPLAHAYGWRTAAGAWAAVTAVAAVLWFIAVPRSAARTAPAPASAAPRPSWRTPMVLGLIGLMAMTTLHVYTLVTWFPAMLEDAGLSSTASALLLSWFAGLGLIAAFVVPPLTKRLRNSYPIVVVCVALMGLGYAGMLTAPAGGAFVWATAFGLGVSTFPLCLTLIGARAADPQSASRLSGLVQGVGYGIGCVGPLTLGAINQLTDDWNATYVLLAATLLITLAAGWTACRPVAAPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVG5</accession>
    <name>U5VVG5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359231.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07055"/>
    <dbReference type="EnsemblBacteria" id="AGZ39700">
        <property type="protein sequence ID" value="AGZ39700"/>
        <property type="gene ID" value="AFR_07055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1439"/>
    <dbReference type="eggNOG" id="COG2129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083277_0_0_11"/>
    <dbReference type="OrthoDB" id="1690667at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016538">
        <property type="entry name" value="UCP008292"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008292">
        <property type="entry name" value="UCP008292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="26015" checksum="1F6873897B39CA0A" modified="2014-01-22" version="1">MIRIAAVGDVHVDKDVLGRYRPALEQLPGIADALLIAGDLTRHGTVEETKCMAQEFGDLGVPVVVVLGNHDHQSDQQDQVADVLRDSGMTVLEGDGVVLEVRGHRLGIAGAKGFGGGFAGACASSFGEREMKNFVGTTEAIADKLGDALRSLECDALVALTHYSPVPETLVGEPLEIYPFLGSYLLGQAIDSAPTALALHGHAHHGTERGRTPGGVPVRNVAHPVIKQAYNVYQLLSEDVDAGLVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVL9</accession>
    <name>U5VVL9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase_M48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_06935</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06935"/>
    <dbReference type="EnsemblBacteria" id="AGZ39676">
        <property type="protein sequence ID" value="AGZ39676"/>
        <property type="gene ID" value="AFR_06935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1415"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017915_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M48" evidence="8">
        <location>
            <begin position="51"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="221"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="310"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="754" mass="76862" checksum="8E2ED044D2C4E929" modified="2014-01-22" version="1">MWALLDGAAQAAGTRAPDRVTVVADAAAKIIERTRLLGLIGGRRDLYLGLPLLQAWDEAHLRAVAAHELGHYSPRLGRLAPLAYRGRVAVARTVPRIPRRNPAGPLLRAYAKFYRRVDAPFSRAQELTADQIAAEYAGRSAAAAVLRDLPALDAVQRIFHAEYLGPGWQAGHVPDDIFGGFLRVLAARADDVAVLRARDPGTPSSWDTHPPVTDRLAALSLSTPPATPAPRPAPTGTAPAPGEPARPTDSGSEIADRPDVTNKSVDPTRAADGSSAASARGEDDRSTHGAGNPEPDLVSAAAPTGRPASLADAPTSTNPGGRDDAPNASASTTLTDATSTGRSDSSTASGSPQPSDAPGPAAFGNHGDAPTASGSSDHGDAPTATAFGDRGDASASAISEGRDGVSTAAASGGRGGAPAQRASTGSDEPSERVGRGEAPAGGAELGGRTSPEAGGPGAPAGDLVPDLPGLGRALQGVAFPPQGRTTVSWDVFFGAARTVEMQREAEAALVTISRAAGAPVGNAADVLDLASDGRLHKAAESVFTGLSAEETASRVAELVTLLLALAALHSDAARWRHSWTGTAELVAADGAHLDLGGLAALASDPATVGTARERLAELGIDPAAEPGADVARTRPEVVGGVVNLVVDGSRTDVLIVDTGLFLVPGLPRSQNGTAKRRLVQFAAADVPERDGAAPGSRFVPYAEVAGATRTKRRSWDLSLRNGGTMSVRAALDSDELPGGWAALDDAVAFLSRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW79</accession>
    <name>U5VW79_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Robl_LC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359447.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08135"/>
    <dbReference type="EnsemblBacteria" id="AGZ39916">
        <property type="protein sequence ID" value="AGZ39916"/>
        <property type="gene ID" value="AFR_08135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1660"/>
    <dbReference type="eggNOG" id="COG2018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118613_0_0_11"/>
    <dbReference type="OrthoDB" id="1736176at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="2">
        <location>
            <begin position="8"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="16999" checksum="D397841CBECA616E" modified="2014-01-22" version="1">MTVDPAVLTELGNLRNRVPELSGSVLATADGMVVAHDAHGIEPDSLAALAAAHLALARRFAHAVNHGELRESVVECDGGYITSYTAGPNALLTLVTSGNANLAMVHLEARRAVRRLVKILILEAPAPVRPEIPTQMGPSTPLARRTPMATLQSTFRGRQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWA0</accession>
    <name>U5VWA0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361183.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14210"/>
    <dbReference type="EnsemblBacteria" id="AGZ41124">
        <property type="protein sequence ID" value="AGZ41124"/>
        <property type="gene ID" value="AFR_14210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2883"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_0_2_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23172" checksum="6A9BDE9864E39406" modified="2014-01-22" version="1">MEAVPVWLRERRTGVGRPAERSRAEVTAAAIALADREGLAAVSMRRVAAELGTGAASLYRYVDSRDDLLDLMTDEVAAAYQFPEPGGPWLPALLEVGRQGHELMLKHSWLPELVLTRSALGPHGTDLLEHVLAVLDGHPADVGRKLEAFAVLTTLAAAMALNKRNEGARQRQGAYLAHAIAAGRHPHLTAALTGGSLVSAPRFEDTLSRVLAGLLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWN8</accession>
    <name>U5VWN8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361348.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41289">
        <property type="protein sequence ID" value="AGZ41289"/>
        <property type="gene ID" value="AFR_15035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3053"/>
    <dbReference type="HOGENOM" id="CLU_406913_0_0_11"/>
    <dbReference type="OrthoDB" id="415763at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665285" evidence="1">
        <location>
            <begin position="26"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="675" mass="72045" checksum="7C5652047BDC46E1" modified="2014-01-22" version="1">MPASRRLLAGALAAVLVSGAGCSLFGEGDRTATFHDGGAAPTTVTVPAEAGEPRISALPAAAPATLPPGTRALASPVEVETPRALEGTATITMTYDPAAVPDPAELAVYTFVEDLGIWLPAGNTLDTGKHTVSASTEHFSRWMLAVTDPDRLRYDQSVTERLKRTTGGSIVSLMYGEPRALGCDAGRVLVPAAVRSDLIVGPAKLCQQIMDDGSYELEWINNTELPIVFDLPAGFTETTTDYRLNPMLQGVLQQHRRPGGAIVLPDRSLKVAFGAGEDAEIRGELDWSIYFIALMRQIVEIAMLDESTDNPQARKKLDAAFEGAGWADCAGVGVNEWNDSHDVAKAVRKAVWDCKDKIARLVLDTFVKVGKGAFDSLKKFFGKRLDVLLGLDKLMAFARSEIAGLLALPGALTGALDTTVTVRPGRVMTYDDAMRLPEARLFDDTIPCNPLPSGVWPPPGLPAGRICVQAVKLDLDGNGRPDRLLTWRPPSPGPGIDVDAKRTGAAAYLDDGTFHQLETPVARWPGIEGVDLFDVAAVVHLGPDPRAQALVATGLGSSTLHQAILAVGTDRRLRPVTLNDAVADIASGGAAAYGSAWGCALSRKKPIFVQTYWSNDVTNPRARREWFRSFFTYAEGRLTEIGGDKGTIAPTKLPVTGSNCATAAVARRGPEIKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX37</accession>
    <name>U5VX37_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09635"/>
    <dbReference type="EnsemblBacteria" id="AGZ40216">
        <property type="protein sequence ID" value="AGZ40216"/>
        <property type="gene ID" value="AFR_09635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1964"/>
    <dbReference type="eggNOG" id="COG4923">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080977_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007362">
        <property type="entry name" value="DUF429"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04250">
        <property type="entry name" value="DUF429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="221" mass="23810" checksum="0907398883F94076" modified="2014-01-22" version="1">MIGVDAYALGWVGVELRDGSFGRAMLASTLYEIVAGSSGAAVIGVDIPLGMLPDGWRAADTLAADQLGPRRSSVFRVPPKAVWQEDDFAAANRLCRELTGAGLSRQSWALRPKLLEANAIWERHPGLLFEAHPEVSFRQMAGEPLAYAKKTWTGQARRRELLARHGIVLPDQLGPAGQAPPDDVLDAAAVAWSAHRMATGAAQSHPSPPEENNGSRIAIWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXB6</accession>
    <name>U5VXB6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361673.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16600"/>
    <dbReference type="EnsemblBacteria" id="AGZ41598">
        <property type="protein sequence ID" value="AGZ41598"/>
        <property type="gene ID" value="AFR_16600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3366"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075562_0_0_11"/>
    <dbReference type="OrthoDB" id="1024950at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="197"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="34266" checksum="D875A318D3428CAF" modified="2014-01-22" version="1">MNILLWHVHGSWTTSFVQGKHRYLVPVNAERDAWGRGRARTFDWPDTVEEVDLAEVTDVDVVILQRPEEVERVAGIRAPKIYVEHNTPKGDVPETRHPLADRDDLIIAHVTHFNELFWDTGATRTTVIEHGIVEPAVRWTGDLDRLAVVTNEPVRRGRVTGTDLFPRFAAVAPLDVFGMGVAGLTGDGITAFDDPPQAEMHAAVARRRAYLHLCRWTSLGLSLIEAMQMGMPVLALATTEAVAAVPDDAGVLATRVDTLVEAARWLIEEPEAARRFGDRARQVSLIRYGLDRFLADWDRLLLEETCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXJ7</accession>
    <name>U5VXJ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_16435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361644.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16435"/>
    <dbReference type="EnsemblBacteria" id="AGZ41569">
        <property type="protein sequence ID" value="AGZ41569"/>
        <property type="gene ID" value="AFR_16435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3333"/>
    <dbReference type="eggNOG" id="ENOG502ZIHR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_570657_0_0_11"/>
    <dbReference type="OrthoDB" id="2200521at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="478" mass="53031" checksum="0F2A4B45B9FAA2EC" modified="2014-01-22" version="1">MTLPLELVTDPAGAEFRSLLNRLGRRGAIRVARADLGTLADYLDQRFGLPPAGTVEERLSAAIQVMADREEIQARYLSNHDHLATYCERAGITYERIWFDGERWLLSHRCVPLFRSVEPNGFLRTLTAVLPRLGSRSEDFRLVESFSDPATAHPDDIYTAYGHTVWQAYEDFGPVADLLEARFGLPHREHVDDRAATILTGLAERGELGPDLPPGANGRRFADWCREAGARPRLDGRTRTHVLRTGEPVLEVAFWPHFGPSPFVRFTEAYRTGDLRHGGTQYHVDAPLDDLPRLVEEFERRLGLEPATGRADDRFIACLEVLVARIGLLSRQGSRALVAEWITASGVTPTVTGFARTEQLVHVHRSSTDCIYELVLTMDATAEITFAEKYEYLPQAGDAGREYSYGVRTPYASLAALVAYLGGGAGEEGLTRGFRALVADGTLTAELGLEEARDRVAEVFRRAGVPAELTDSHWINSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXY1</accession>
    <name>U5VXY1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative pectate lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_17875</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362179.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="SMR" id="U5VXY1"/>
    <dbReference type="STRING" id="1246995.AFR_17875"/>
    <dbReference type="EnsemblBacteria" id="AGZ41853">
        <property type="protein sequence ID" value="AGZ41853"/>
        <property type="gene ID" value="AFR_17875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3626"/>
    <dbReference type="eggNOG" id="COG3866">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021894_2_0_11"/>
    <dbReference type="OrthoDB" id="660314at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PTHR31683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 2 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665841" evidence="3">
        <location>
            <begin position="35"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Amb_all" evidence="4">
        <location>
            <begin position="60"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="33843" checksum="C9E19330FD5CD222" modified="2014-01-22" version="1">MRKHLRQLVLGASAGATALALTGVGIIATTSASASTPVPLAAVDQLVGWATQNGGTTGGGSAATTTVTSASALTSAVGSTSASVIRVSGTINCSGMLRVRSNKTIIGNSGAAIVGCGLNINGDRNVIIRNLTFRNWNDDAINVQTSATNIWIDHNTFSNGYDGAVDVKRGSDFVTVSWNRVFNHDKAMLLGHSDDNASQDTGHLRVTYHHNWFDASTQRHPRVRFGNPVHVYNNFYYRNSGYGVASTEGAGVLVENNSFDGVSDPYHLGEGDSGPGTLVARGNLLVGSGSGQTGGSVKTIPYGYTLDAAGNVKSIVTASAGAGRISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXY6</accession>
    <name>U5VXY6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362189.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17900"/>
    <dbReference type="EnsemblBacteria" id="AGZ41858">
        <property type="protein sequence ID" value="AGZ41858"/>
        <property type="gene ID" value="AFR_17900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3631"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_6_2_11"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="PlsC" evidence="2">
        <location>
            <begin position="75"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="26946" checksum="E27B6B8242C3C2A3" modified="2014-01-22" version="1">MLRALSVLFWTVIVVTCPVFFLGALVVWAVCLPFDRRKVALHLYSSAWAVFYVRLNPLWRLRTSGRELLPWRGAAVLVANHASLIDILVLFDLFRPFKWVSKAEIFRVPVIGWNMRLNGYVPLVRGSGASVRRMLDRCSELLSAGSPVLFFPEGTRTDDGLLKAFKDGAFDLAVRHDVPVFPIAVHGTGRALPKHGLILRDHVDARVEVLPALLPADHADVAALRDAARDRIEAALRARTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYH4</accession>
    <name>U5VYH4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360755.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12045"/>
    <dbReference type="EnsemblBacteria" id="AGZ40696">
        <property type="protein sequence ID" value="AGZ40696"/>
        <property type="gene ID" value="AFR_12045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2452"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026152_4_2_11"/>
    <dbReference type="OrthoDB" id="848990at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="189"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="40178" checksum="BBD5F76C481B8DE1" modified="2014-01-22" version="1">MRRLGGITARGLGVLLGAVALLVAGFRFGYPDLALLGSAAAVALICGGVFAVWRPGLGVERVAEPDRVARGEPANMTLTVRNTSKLRAANLVAVDRCGPANVPVPLLRLRPGRDTTVGYPVPTNRRGVVPVGPLRVTRNDPLGLMTLSRTYGESTTVWVHPRIHLLRAVPAGMARSLDGRIDKVPHGTITFDSLREYVVGDELRRVHWRSSAKVGELMVREQLDTSEPTIIVLLDDRAGAHPRGKDGVADSFEDSCEAAASIIAAAVREDLPVSLHLVSGPAKGPFLDVLTEATLRAGDLTQTLRSLRQSKLGDTLVYLTGPGGRADLGAVTALRGAYPVVIAGLFGDPDAAPVSAGGGLIVIEAENGEQFAGAWDGVRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYV4</accession>
    <name>U5VYV4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transporter inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18445</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18445"/>
    <dbReference type="EnsemblBacteria" id="AGZ41967">
        <property type="protein sequence ID" value="AGZ41967"/>
        <property type="gene ID" value="AFR_18445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3743"/>
    <dbReference type="eggNOG" id="COG4176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023562_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="454"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="497"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="576"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="609"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="149"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="450"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="641" mass="67229" checksum="9518F626890E8328" modified="2014-01-22" version="1">MTATVAPAEAVETTELSGPEEPARPKVRVSRRTVVAVLVVATVVAYLSLRNGGAAEEPDAWAFRFFSGVRDWVDDNRDTSPVFLYGVNYLRLGVVELVGLVQSLLYGLGWTGLVATVGALTAVLAGWRLAIGAVAGFLFLGVLGLWEESVDTLVLTLSAVLLAVLIGVPIGILAARVPAVGAVLKPVLDVMQIMPTFAYLAPMTLLFSIGEPAAVIATLIFAIPVTIRITALGISEVSPTTVEAATALGSTRWQLLGKVRLPMARPTIVLAVNQTIMMALSMVVVTALIDAPGLGQTIVTALERVNVGVAFDAGLAIVVLAVVLDRITTKAAGRRPRLPYVAGALVLTAVLALLPLSSEFPERWQFSFAGPVNDITSWVEFHWYDATEALKNVISAGLLDPLQTVLVTTPWWLFVVVVLVFGSLASGWKSGVIAALAAAGISGLGLWQHAMQTLATVLVGTVATMAFGVLLGVLCARHDRFARGLRPLLDAAQTMPSFVYLLPAVALFGASRFTAIVAAVIYAVPPVVRLVERGVRDVPPTVVEAALSAGSTPRQLLWKVQLPMARGGLLLATNQGIVMVLAMVVVGGLVGAGALGYDVVTGFAQREDFGQGLAAGFAIVLLGVLLDRLTQGLHSAKGTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYY1</accession>
    <name>U5VYY1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ornithine cyclodeaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362365.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="SMR" id="U5VYY1"/>
    <dbReference type="STRING" id="1246995.AFR_18570"/>
    <dbReference type="EnsemblBacteria" id="AGZ41992">
        <property type="protein sequence ID" value="AGZ41992"/>
        <property type="gene ID" value="AFR_18570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3769"/>
    <dbReference type="eggNOG" id="COG2423">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_3_2_11"/>
    <dbReference type="OrthoDB" id="1193876at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="PTHR13812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="36247" checksum="31F04E8A641CD4A7" modified="2014-01-22" version="1">MDTLLLTRQHVAKLVEMNGRDHFMDLLIRNLRTAFRAESKGHTPPRGGFLRCPGDTGVIEWMPHHRAGESMTLKLVAYTPSNPERLNLPTIIGTLTRYDDVTGHLEAVSDGVLLTAFRTGAASAVASSLLARPDSRTLGLVGAGAQAVTQAHALSRIFPLERILVTDAEPSHAASYADRVDFLGLDVRVADVEEIERESDIICTATTVGVGAGPVLPGTSLRPDVHINAIGADLVGKFELPVAVLRSATVVPDHFAQARAEGECQQLADDEVGPDLMTLCARPQEAVALRSGRTVFDSTGFALEDHVAFSTLIELAAEAGVGDRVALEHLPEDALNPYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ12</accession>
    <name>U5VZ12_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two component transcriptional regulator, winged helix family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_13150</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360975.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13150"/>
    <dbReference type="EnsemblBacteria" id="AGZ40916">
        <property type="protein sequence ID" value="AGZ40916"/>
        <property type="gene ID" value="AFR_13150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2670"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="24593" checksum="0AF7A79A256CE84E" modified="2014-01-22" version="1">MRLLVVEDERELAESLRRGLVAEGYTVDVAHDGVTGLEYALTEGYQAIILDLMLPRLNGFLVCARLRESKVATPVLVLTAKDGEYDEAEALDTGADDYLTKPFSYVVLLARVRALLRRGGEPTPALLRVGDLVVDQAQRRCSRGSTAITLTAKQFSVLACLARRANAVVGKAEILDEVWDAAYDGDVNIVEVYIRALRLGIDVPFGVRSIETVRGVGYRLVDGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ71</accession>
    <name>U5VZ71_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_20055</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362655.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20055"/>
    <dbReference type="EnsemblBacteria" id="AGZ42283">
        <property type="protein sequence ID" value="AGZ42283"/>
        <property type="gene ID" value="AFR_20055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20055"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_23_0_11"/>
    <dbReference type="OrthoDB" id="1210653at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="1"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="399" mass="41468" checksum="62739DE2C5477BE4" modified="2014-01-22" version="1">MTFLLALACGIAVGNVYFPQADSPSIAAGLHVDPAAAAAVVTAVQAGYTAGLFLIVPLGDRFRHRPLIVTLFALTGTALLAASLMPALLPLVAASVVVGVCTVAAPVIGPMAAGLVPPGRQGVTSGILLSGSTAGMLLSRTFSGYLGEWFGWRVPYLGAGLLALLMAVLLARTLPATRQPVVRLRYPALLAEPLRLLRTEPELRRSCVFQATVFAGFSAVWTSVALLLTGPAYGLDARAVGLLALVNAATMLCTPVAGRLVDRHGSNAVNLVCLGGVIVAAVVLAVPGLGFLIAGTLLLDVFMQCGMVANQVRIFRLPAEIRSRLNTAYMTCAYTGGAAGSWLGLRAYAHFGWLGVCTLVAVLAGLALTRARFATGRRTSRPRRPARRGFPAPPGDPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ78</accession>
    <name>U5VZ78_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase sigma-70 factor, ECF subfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_13525</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361050.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13525"/>
    <dbReference type="EnsemblBacteria" id="AGZ40991">
        <property type="protein sequence ID" value="AGZ40991"/>
        <property type="gene ID" value="AFR_13525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2746"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_2_11"/>
    <dbReference type="OrthoDB" id="1992113at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="23"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="118"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="190" mass="20947" checksum="3AFFB83385456C76" modified="2014-01-22" version="1">MASDADLIGRSLNGDVDAFVEVVERHEAAVGSYLARRAGRDAAEDLLGEVWVAAFASRQSYDQSYGDARPWLYGVALNRIRRYFRSRPAEDLVADVTDLATGWDPWSAVDLGMDARAVLRSALLRLKPEEREVLRLVAWEDLTAADAARTLGMPAGTARRLLSQARAALRDAPGVSALLKERNSAKERHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ82</accession>
    <name>U5VZ82_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_20130</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20130"/>
    <dbReference type="EnsemblBacteria" id="AGZ42298">
        <property type="protein sequence ID" value="AGZ42298"/>
        <property type="gene ID" value="AFR_20130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4083"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="12"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="451" mass="47435" checksum="71477DDD61D37D88" modified="2014-01-22" version="1">MLQVGQRLGGRYRLEVRIGAGGMGEVWRAVDEVLGRVVAVKAMLPRIADQPDFARRFLVEAKAMASVNHGAVAAIHDYGSDDGITFLVMEFIDGESLSQLLGRAGRLGAADTMHLIAQAADGLQAVHDRGIVHRDIKPANLLVRRDGSLLITDFGISRTHDGTQLTVSGAVLGTPTYLSPEQVLGRPATAQSDVYSLGLAAYECLAGQRPFGGENPYAVALQRLQAGPPPLGADVPRAVAAVVDRALAIEPADRWQSAAELATAARAAMHGGSPQAVAVRRRAVPGRRWAIAAICLVILVVGGVTAWQVFDTGESPSIAGAPVSNATPPGLEACGPVFCPVEPLCWSGLTSISGRAEPPWPADCAESHVWETFAAFPPPAAAAKMREDELMTNEAVAGVCTAALMSSRSQDRTMTEGWVRDGWPIRVDGGGPRLIHCLARPESGETTGAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZV6</accession>
    <name>U5VZV6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_14840</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14840"/>
    <dbReference type="EnsemblBacteria" id="AGZ41250">
        <property type="protein sequence ID" value="AGZ41250"/>
        <property type="gene ID" value="AFR_14840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3013"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_135_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="523" mass="54294" checksum="FE3748384A307A31" modified="2014-01-22" version="1">MGGYRLLGRLGQGGMGAVYLGRGAEGRLVAVKMIRPEFAYDTEFRGRFRSEVNRARQVPPFCTAEVLDADPDHSTPYLVVEYVDGPSLAQVIAGQGPLSGGSLHSVAVGVATALSAIHGAGVIHRDLKPGNVLLARGMPKVIDFGIARAFEATSQHTRTDQMVGTVAYMAPERFDTDGGAVGPAADVFAWGAVVTYAATGRTPFQADSPAATAARILTQPPELSGLPEPLRSVVTRALAKDPAERPTAPQLLNLLLDLDAADTTELLRPELLRTAVAAQRAPGRTGKGSKRRIRAAVVAGVGALVLAGAGLLAAQALPGLGAATDDVIGDAAPASARPSLSRAAGGTEIFDRLDRPGQWKDTRTDADGRCLFTDGRLEARTGMSPVYRCQGPADSFAKDQAVSVDATVLTPGSCAVIWLRATGADAYLVSLCESEVRLGVDAADGVTGEVRAAVTGTGLGRARRVEVVVRDEKATVAVDGTEVLATPLGEASLKAGRVTFGVIDDIISGDARAAFTQAKIMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W094</accession>
    <name>U5W094_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15295"/>
    <dbReference type="EnsemblBacteria" id="AGZ41341">
        <property type="protein sequence ID" value="AGZ41341"/>
        <property type="gene ID" value="AFR_15295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3106"/>
    <dbReference type="eggNOG" id="COG3250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005015_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="SSF49303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="910" mass="100354" checksum="EB135AB93B1B7252" modified="2014-01-22" version="1">MIKVADLGGAWTLSGPSLSGPSLSGPALSGPALSGPSLGDEGLPAVVPGCVHVDLMNAGRLDDPFLDDNEKAAAWVGRADWTYTRDISWDGPRPERIDLVFDGLDTVADISLGGVALGSTRNMHRSYRFDVTSLVGADPVPLEVRFTSAYTEAEAVRTQLGPRPNAYPEPFNYVRKMACSFGWDWGPTLVTAGIWRPVRLEGWSTARLGSVRALTTYRDGVGVLDLDVDLERTQARPLRLRVLLDGRELAVVPVPGERRPDFKEVVPGQAERQPDFKEVGPGQAERQPDFNEVGPGQDERQPNFNEVGPVPDERRPNFKEVGPGQGERRPNFKEVGPEPSEPMGNFKEIGPGQAERQPDFKEVGPVRIDVPGVQPWNPGAANLYDLTVVLLDGDGDGDLELDRWERRTGFRSVEIDRSGDGFVFHVNQKPVLVKGVNWIPDDIFPSRMTRERYALRLGQAAAAGVNMIRVWGGGIYESRDFYEVCDELGLMVWQDFLFACACYPEEEPLYSEVVAEARENVSRLSSHPSLVAWNGNNENLWLYRASGWDTEPGGDSTWGERYYLETLPEIVAELDPSRPYMAGSPWSGSWEHEPNSPEHQTFHSWEVWNRQDYVHYRDSTPRFVAEFGWQAPPAWRTLRDAVSDEPLLPDSPGVLHHQKADDGNGKLARGLAPHFPAPDSVEAWHYLTQLNQVRAVRTGIAHWRSYWPHTAGTILWQLNDLWPVTSWAAIDGAGRFKPLYFALTSLYADRLLSIEPRDGGLVVAVMNDTDAPWAGSLEIAAMTTPISVSPRSVSVVPVPADLVPSDFLVARLDDVRDVWFAAETSGDPGLTVTATPVPGGLDVHVRSAGLARDLLLQPDRIHPDAVVDRGFVTLLPGESTTFAVRCPAELDPASVKSPWVLTDLASVLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0J2</accession>
    <name>U5W0J2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_16115</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361580.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16115"/>
    <dbReference type="EnsemblBacteria" id="AGZ41505">
        <property type="protein sequence ID" value="AGZ41505"/>
        <property type="gene ID" value="AFR_16115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3270"/>
    <dbReference type="eggNOG" id="COG0334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_0_0_11"/>
    <dbReference type="OrthoDB" id="1184827at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016211">
        <property type="entry name" value="Glu/Phe/Leu/Val_DH_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42722">
        <property type="entry name" value="PTHR42722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000188">
        <property type="entry name" value="Phe_leu_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="4">
        <location>
            <begin position="137"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="175"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000188-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000188-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="35639" checksum="A30B21C0EA69A88B" modified="2014-01-22" version="1">MTLNSRPETVRVQRGTRTGQMIIVSVDSTVLGPALGGCRIRTYPAWRDGLDDALRLSSAMTEKAALAGLPYGGGKTVVALEGPEPRRADLLADVGDLVESFGGRYLTGPDVGTSPDDMTVLRRTTEHVLCRPESEGGSGDSSGPTAAGVLASIEAVRAHRFPGRPLESLSFAVLGLGHVGTLVASHLAAAGARLTVTDIDASRRTVARGWGATWIDPDRALGAEADVLVPSAVGGILCPDTIGTLRCGAIVGAANNQLDADTTADLLHARGICWAPDTVVSAGGIVSAIARERDLVSPDEAARRVGDIGRRLGEILAAAARDDVAPLREARRQVQDLLTPPMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W197</accession>
    <name>U5W197_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363218.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22585"/>
    <dbReference type="EnsemblBacteria" id="AGZ42787">
        <property type="protein sequence ID" value="AGZ42787"/>
        <property type="gene ID" value="AFR_22585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4578"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_0_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="322" mass="33984" checksum="957A97EBD7BB938B" modified="2014-01-22" version="1">MITTRFDGTSTADDVVAGLDLTGVRAVVTGASSGIGVETARSLARAGAEVTLAVRNTDAGARTAADITRSTGNGAVRVGALDLADLASVTAFAERWTGPLHLLVNNAGMIVSTLQHSPEGWELQFATNHLGHFALSAGLHEALAAGARERGEARIVSLSSTAHMRSPVVFDDLHFERRAYDPQAAYAQSKTANSLFAVEATRRWAADGIFANTVNPGGVSTGLQRDFTQKQKDYLAAAEASGAFTYKTVQQGAATTLVAAVAPEFVRTGGHYLDDGREAYTVGNEADLFEHSHGVKEWALDPGAARRLWALSLDLTAPHRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1M7</accession>
    <name>U5W1M7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363470.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23285"/>
    <dbReference type="EnsemblBacteria" id="AGZ42927">
        <property type="protein sequence ID" value="AGZ42927"/>
        <property type="gene ID" value="AFR_23285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4719"/>
    <dbReference type="eggNOG" id="COG1502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460616_0_0_11"/>
    <dbReference type="OrthoDB" id="1881748at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PTHR18896"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="2">
        <location>
            <begin position="155"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="2">
        <location>
            <begin position="411"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="537" mass="59941" checksum="3D8CE8E55AB392D0" modified="2014-01-22" version="1">MPHALTLSELIDRYLPPATEVCPTFTENSVYEPIIDGVTYFSALAEQMDALGPGDAVYIAGYQADPQLDLSGRVAGEPGYRPFTDVLAEKAADGTDVRIVLSAAEFSGGVPWLPVGPFRANTLAARVMRAWLPSNREVAEPPLHDRVLLDWSGPLIGSNHQKFVVFCHDGELTAYVGGLDLSPTRFDKSPHHHLRLGVHRWGWHDAAARLHGPAAVRVRETFRSRWENAAILPPRKIARNLPAQYFRLPSGGGLTVINPPDTPLTLPPVPEQPDRPADGTAVQVLRSYRPWSLYRHRGLRRMRRANVTRAGIQEVYRALVTAIRGAERYIYLEDQYFHEAPGGDPRFQLYGELRAAACRGVKIILIGSGRRDPADGGDSTVRPVITRDLRRRVLDQLSPESRRNVVMYRVDNLTVHTKLMLVDDVFISIGSANFFSRSMVGTDSEITSTMVTTGHAVRDLRVKLWAEHLRAPLNDEVTPVLSDLDTALGIWRTEWLPDGHPPETWRRAGSPGGFHPSERALIPARYSSFVLRRESRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1N5</accession>
    <name>U5W1N5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_23335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363495.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23335"/>
    <dbReference type="EnsemblBacteria" id="AGZ42937">
        <property type="protein sequence ID" value="AGZ42937"/>
        <property type="gene ID" value="AFR_23335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4729"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_631368_0_0_11"/>
    <dbReference type="OrthoDB" id="1445278at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="24"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_transf_7C" evidence="3">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="429" mass="45789" checksum="849A72597E578939" modified="2014-01-22" version="1">MVTVPGNRWDLLDGLEPTEPPTVSVIVVHYRQQAELDRTLAALRRQTHPAGRTQVIVVDDGSPEPPRVPDGVQLLRQADDGFRAAAARNLGASAATGEVLCFLDADTSPEPEYLTRMARLPALAPEAVVVGRRRHAALAGLPADTPVEHAGPAAELPEPQWLIDAYRQTRNLLDADDRAYRYVIGAAFACSRWFFERTGGFDEQFRAYGGEDWEWTYRAWLGGAILAHVRDAVAWHDGPDWAGRGTDDPERRARKDGETLLLADHIPVAGSRGHGLRSASPDVVIRLAAALSPAAAFVCVDSLLAALPEAVVQVPGDFKGVFSADQRVTTASSLLDPRTRVVIDVPAAVRADRAADLLRDAVSTVGAGAVGTVLLGPAEAPLLRVDSARAAARRAAWGKDVFETTVLPDADLTVLGADPGLAGYLGGWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W285</accession>
    <name>U5W285_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1775 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_25420</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25420"/>
    <dbReference type="EnsemblBacteria" id="AGZ43348">
        <property type="protein sequence ID" value="AGZ43348"/>
        <property type="gene ID" value="AFR_25420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5150"/>
    <dbReference type="eggNOG" id="COG4549">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1021695_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012533">
        <property type="entry name" value="YcnI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038507">
        <property type="entry name" value="YcnI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07987">
        <property type="entry name" value="DUF1775"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1775" evidence="3">
        <location>
            <begin position="68"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="308" mass="31458" checksum="530ADE48C675205C" modified="2014-01-22" version="1">MSLSIATSRMPTIMPNPPKESSQRLNHSAASTELPVVRHLEKHRRAGVLAAAVATGVLCAASPAAADVTVSPPTAPQGSGTNVTFRVTNTAQSAITRVKLVLPADQPVAEVYPLSVDNWAPQIVQRDLDKPLTSIHGGQPVTETASAIVWIAMPGKSLAPGKSADLAVALGPLPVETTQMSFAVEPTYADPAKGAAMPPAQLALTPAVPGQEGAAHGGHSGTGTSGTSDADAALFAALLDENDGPGFWTIAGWVVAALAAAAGVIAVLRSRRRNEPAASDDSSEDPPKELVGAAPRVTSWSYKDGPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2B8</accession>
    <name>U5W2B8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative trypsin-like serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363869.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25065"/>
    <dbReference type="EnsemblBacteria" id="AGZ43279">
        <property type="protein sequence ID" value="AGZ43279"/>
        <property type="gene ID" value="AFR_25065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5079"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_3_5_11"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="27938" checksum="FD81E2345622290A" modified="2014-01-22" version="1">MTDLLARPPYSQDAPPPFPPLPPNAVSRRRWPRRFAGGAVILALIAGGGTAGGLIADHYAGTTPAASTVVQATGTPVVAQNNSLAAVAAKVSPSIVTVVLDSAAESSLGSGVILSADGLILTNNHVVASGGTASVRLSDGRTVPARVVATDTTHDLALVQATGLSGLIPATFGTNDSVAVGDTVLALGAPLGLEGTVTSGIVSALDREVSTGPEKLTGLLQTDAAINEGNSGGALVDTSGNVIGINVAIATAGDDSTGNVGVGFAIPADTVTTVINQLEAQASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2F0</accession>
    <name>U5W2F0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase AAA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362449.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19010"/>
    <dbReference type="EnsemblBacteria" id="AGZ42076">
        <property type="protein sequence ID" value="AGZ42076"/>
        <property type="gene ID" value="AFR_19010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3860"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053995_2_0_11"/>
    <dbReference type="OrthoDB" id="688156at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="36"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="389" mass="40767" checksum="C53EF1422E201483" modified="2014-01-22" version="1">MSALDGADALIAATRAHRTEPSADPRVEALALAVSANLPVLLWGEPGIGKSATLQQLATGLGLPMETVIASVHEPSDFAGLPIVGDSPATDGVPMAPPDWAVRLARTGSGLVFFDELSSAPPAVQAALLRVVLERRVGSLQLPDPVRVVAAANPPTSAADGWHLAPPLANRFVHLQWTHDPRTVARGLAGTWPAVTVPTVRPERARTSLARARGALAGFLTARPGLTHHLPEDAESRGGAWPSPRTWEMVLRLLTFHYSARTSTSALALAVAGAVGPGAGMEFVSYLENLDLPDPERVLADPSAFELPGRGDRQLAFLTAVIAAVQAEATRPRWEAGWVVLQKAVDAGVPDVAARAAADLAALREPQWPVPATVDAFIDVLSLSGRLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2N7</accession>
    <name>U5W2N7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Aminoglycoside/hydroxyurea antibiotic resistance kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363867.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25055"/>
    <dbReference type="EnsemblBacteria" id="AGZ43277">
        <property type="protein sequence ID" value="AGZ43277"/>
        <property type="gene ID" value="AFR_25055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5076"/>
    <dbReference type="eggNOG" id="COG3570">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061172_2_1_11"/>
    <dbReference type="OrthoDB" id="1457558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006748">
        <property type="entry name" value="NH2Glyco/OHUrea_AB-resist_kin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04655">
        <property type="entry name" value="APH_6_hur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="32457" checksum="AF5730215B8F3429" modified="2014-01-22" version="1">MLDIPATVRAKARQEPGWLESLPGLVDELAVEWSFTPGRAYQDGTEAYVAEAVLVDGTPAVLKLLVPRAGGTHAAAEITVLRLAGGDGCVRLLRSDAARGAMLLERLGPSLADLDLPLDERLEILCDLAQRVWRPAPDSGLPTGAEKGRWLVEHIRQRWEDLDRPCSERTVAHAIACAERRIAAHDDARAMLVHGDVHQWNALRAGDGYKLVDPDGLLAEPEYDLGVLMREDPVELMQSDPWDRAHLLAERTRTDATAIWEWGVTERVSTGLLAVDIGLQPVGDQMLAAAELISVSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2X4</accession>
    <name>U5W2X4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364229.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26110"/>
    <dbReference type="EnsemblBacteria" id="AGZ43484">
        <property type="protein sequence ID" value="AGZ43484"/>
        <property type="gene ID" value="AFR_26110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5292"/>
    <dbReference type="eggNOG" id="COG1649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057304_0_0_11"/>
    <dbReference type="OrthoDB" id="538762at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="362" mass="38201" checksum="4E0C3628A69AFEB9" modified="2014-01-22" version="1">MVSGHAYPWDVLGDPDFVARAATAVPSVTLAATYHSTRAATPLHPARQIVDARYAALYRPVRESAWKGQLLRAAAPDWLDEKDPFGEAAEVLRSAGLAVTAWIVLTHNTRLGLENPSVTVVNCFGEHYPYALCPAHPAVRAYAATLAAEAVHGLDVDGISLEACGQLGLQHLGHHEKTDGAWSDLAVRLLSICCCAACRSGWTNPDTIVATLREAVRASVEPPAELLAEVLANRQANTDALRAEVLAAVRSPGRPITLHANPDPWATGPSPGLTPAAAADVDALLVPAWPTTLASADLVRQAAATGVPVDAYVTVLPPADPGVLPAHVDRLRAAGASRISLYHLGLAGRGRQDLLREIASRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Y5</accession>
    <name>U5W2Y5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364249.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26160"/>
    <dbReference type="EnsemblBacteria" id="AGZ43494">
        <property type="protein sequence ID" value="AGZ43494"/>
        <property type="gene ID" value="AFR_26160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5302"/>
    <dbReference type="HOGENOM" id="CLU_2010346_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="123" mass="12762" checksum="81E2E7C40A93304E" modified="2014-01-22" version="1">MISAPNAVVVSVAAAEFPSEDLLRQLHGELRRAGIPSEQVSGEAEPGTKSTAGAVASLVVAGVLSAAGLRAMSQVLVAAIQRSQKRKIEIRRGDDVLILEGPAVGKLGPAMIEDWLRRNPGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W332</accession>
    <name>U5W332_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_20320</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20320"/>
    <dbReference type="EnsemblBacteria" id="AGZ42336">
        <property type="protein sequence ID" value="AGZ42336"/>
        <property type="gene ID" value="AFR_20320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4120"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665648" description="Histidine kinase" evidence="6">
        <location>
            <begin position="27"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="182"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="243"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="453" mass="48649" checksum="7C979203B35A8C80" modified="2014-01-22" version="1">MAWLRRLSLRARLLIVSVVVLFTGLAAGGVVLVATMNFALLRTANNEALATAEAVADLVNTDSVSDPLPAAPAVRVQVLDARARVLAVSAGADRLVPILYASELEALEDGEGRFIPGDRIGLDGRVRVVVVDAGTTDAPRRVVVARPTADLTQGIQLLRSTLLFTFPPLVALLALVAWRVTGATLRPVEALRTGAEEITGGARPGRLPVPDSRDEIHRLAVTLNGMLHRLDTARGRQRAFVADAAHELRSPLTNMRTELEVAQRLPDTTDWPALADDLLTDVDRLSRLVDDLLLLARADDETTRALVREPETVELGQLLAEVAGRYPAVDYERPAVPLRTRGERDAIGRVVANLLDNAVRHANASVRLSVSADGAYQLISVSDDGPGIPAADRERVFDRFTRLDDARARDAGGSGLGLAIVRELVRRHQGTVALRGASPPPGLRVDVRLPVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3B1</accession>
    <name>U5W3B1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_21065</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0749 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362857.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21065"/>
    <dbReference type="EnsemblBacteria" id="AGZ42485">
        <property type="protein sequence ID" value="AGZ42485"/>
        <property type="gene ID" value="AFR_21065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4270"/>
    <dbReference type="eggNOG" id="COG3879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040273_1_0_11"/>
    <dbReference type="OrthoDB" id="1883872at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010273">
        <property type="entry name" value="DUF881"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37313">
        <property type="entry name" value="PTHR37313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05949">
        <property type="entry name" value="DUF881"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="31157" checksum="642D344F2FFEA42C" modified="2014-01-22" version="1">MTSPDGGDGQRRFAPDFLTELFQNPLDPGYADAAARKARGEGPTGTPKKVLSGISALTLIALGFLLVVAYQQTVAEKPARTQARDTLIDQVESRRTATAELQARADGLRSEVAGLREKQLGGAAVARLRDLEAETGLAPVRGSGAKITVADGPTPVDPLTGKRKNEARVRDTDLQQATNALWADGAEAIAINGQRLTATSTIRQAGEAILVDFRPVSTPYQIVAIGPDSLADKFRDGTAGRFFRALSTRYAMAYEVAEVKNVTLDAATEPNLRQAVPSTPPPAAPPSPSSKASKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3K2</accession>
    <name>U5W3K2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative NADH:flavin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364434.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26625"/>
    <dbReference type="EnsemblBacteria" id="AGZ43587">
        <property type="protein sequence ID" value="AGZ43587"/>
        <property type="gene ID" value="AFR_26625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5396"/>
    <dbReference type="eggNOG" id="COG1902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_0_1_11"/>
    <dbReference type="OrthoDB" id="1064766at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="PTHR22893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_FMN" evidence="1">
        <location>
            <begin position="5"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="355" mass="37752" checksum="B3F05544DD9DC67B" modified="2014-01-22" version="1">MTTAFDGFPVGRYTTRNRIVMAPMTRSRAYGPGATPTELMATYYGQRAGAGLIITEGTQPSPVGQGYMNTPGLHTAEQAEAWKPVTAAVHERGGLIFAQLMHTGRIGHPTLHGAHPVGASPVAAAGQVFTADGPLDLVTPEPLDADGITATIQDFVRSARNAIDAGFDGVELHGANGYLLHQFLSTNANRRDDEWGGTIEGRIRLTVEVAKAVAAEIGADRLGLRISPANPFNDIVEDDHRETYLALLDALAPLGLAYLHVGEGPDTEFTAMLRDRWAGAFILNPYTPGAWTGPEALKQIENGETDLVSYGALFLANPDLPERLAAGGPFNTPDYAKAYGGDHEGYTDYPALTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3X7</accession>
    <name>U5W3X7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_27330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560065.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27330"/>
    <dbReference type="EnsemblBacteria" id="AGZ43727">
        <property type="protein sequence ID" value="AGZ43727"/>
        <property type="gene ID" value="AFR_27330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5540"/>
    <dbReference type="eggNOG" id="COG3250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009935_0_0_11"/>
    <dbReference type="OrthoDB" id="1832114at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Glyco_hydro_2_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="SSF49303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666054" evidence="5">
        <location>
            <begin position="18"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="LamGL" evidence="6">
        <location>
            <begin position="679"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="822" mass="88657" checksum="BE33BF3B56108559" modified="2014-01-22" version="1">MAAVTLLAVGTAGAAGAAPAPEAGPAWHKGIPPIATPWTAEVGPDNALPEYPRPQLVRSRWQNLNGVWEFGKAAVDQAPPVGQELGERVLVPYPIESALSGIQRREDRMFYRRTFTVPTDWKVGKGQRLVLHFGAVDYDAKVWVNGTQVATHRGGYDGFDIDVTDALKGKGPQELIVWAEDLTDATGQPIGKQRRVSDRGIFYQGSSGIWRTVWMEPVAVSSISSLTTTPDVGASQLKIKVNTVRGDGLTVRAVARSNGKVVGRVTGAAGTDLKLPVPNAKLWSPDSPFLYDLEVTLLSGKKSLDTVQSYFGMREIGTKKGADGKLRIALNGKILFNMSTLDQGFWPDGLNTAPTDAGLKFDLQQHKDMGFNAVRKHIKVEPDRWYYWADKLGLLVWQDMPAANTGPIPEPWRDQFESELHEMVKEHASWTSIIAWVPFNEGWGEWDRTATGRIADDVKKQDPSRLVNAHSGVNCCNSKGDSGKGDVIDFHQYLGPASPAPDATRVSIDGEHGGYGLKVSNHMWFGDGQAYEMTPDSATLTRKYVENQRAVLTAAQSCGISAAIYTQITDVEGELNGFWTYDRQVAKMDFTQVRAINQEIIAKADGTATNVPQPPAGTPGLTGIAFYPLDGTTEDAAGDHDATLVGGPAYVAAKNGQGVDVNGSTQFIDTGAPVLNTASDYTASAWVKLDKADGAFQTIVSQDGASNSDFFLQYSGADQRFAMSFTGVRALGPSKPEVGRWYHVTGVRDSVAGELRLYVDGELAGRTGACLPQAAPTGNTVIGRGKYGGNSVDYLDGTVDQVHLYDRALSATEIKALFDSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W413</accession>
    <name>U5W413_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1801 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560100.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27505"/>
    <dbReference type="EnsemblBacteria" id="AGZ43762">
        <property type="protein sequence ID" value="AGZ43762"/>
        <property type="gene ID" value="AFR_27505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5575"/>
    <dbReference type="eggNOG" id="ENOG5032S5R">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130420_0_0_11"/>
    <dbReference type="OrthoDB" id="1278667at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014922">
        <property type="entry name" value="DUF1801"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08818">
        <property type="entry name" value="DUF1801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1801" evidence="1">
        <location>
            <begin position="25"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="14720" checksum="3CB46F93ED210141" modified="2014-01-22" version="1">MSGPATVPTGASVGDFLAAVQDPKRRADAQALCALMAEAAGVAPVMWGPSIVGFGSYHYRYASGQEGDWPVVGLSPRKTAMTIYLSAGFEQEILDRLGPHRLGKACLYLKRLADVDDAVLRELVADAFRKLDGRTLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W481</accession>
    <name>U5W481_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_22255</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363092.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22255"/>
    <dbReference type="EnsemblBacteria" id="AGZ42721">
        <property type="protein sequence ID" value="AGZ42721"/>
        <property type="gene ID" value="AFR_22255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4511"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="229" mass="24986" checksum="2366AC18946E7FC9" modified="2014-01-22" version="1">MSVSVSELTRSFGEKAVIDKLDLSIAPGEFVALLGHSGCGKSTLLRILAGIDLEISGEVTVPRRRAVAFQAPRLMPWKKVWRNVVLGLPGRPDRALAERYLAEVGLTDKATVWPKTLSGGEAQRVSLARALVREPDLLLLDEPFGALDALTRTKAQALVAELWARHDCAILLVTHDVEESLLLADRVLVMDHGGIAHEVTVDLPRPRDVGDPRFVELRAQLLSWLGVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4S9</accession>
    <name>U5W4S9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flavin-containing monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_23685</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363595.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23685"/>
    <dbReference type="EnsemblBacteria" id="AGZ43005">
        <property type="protein sequence ID" value="AGZ43005"/>
        <property type="gene ID" value="AFR_23685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4799"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006909_9_4_11"/>
    <dbReference type="OrthoDB" id="324612at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="507" mass="55361" checksum="5E98F45D3A6C01D3" modified="2014-01-22" version="1">MRIGIIGAGVAGLTTAKTLLQAGHEVLVVDRTPDVGGVWSATRRYPGVTTQSAKATYGFSDFPMPRDYPEWPTGAQVQAWLVAYAEKFGVDRHLRLNTEVLRAAGDNAGGWTLELRDTRDGVNSTETVDRLLVANGVFCEPAVPQFAGLDEFTAAGGQLLAGTEVQDAGQARGRHVVVVGYGKSACDVTVELSATAASTHVIARQLLWKVPRKIGGVVNFKFLLLTRLGEALFRYAHLRGMEKFLHGPGNGLRRRMLNSLGTASVRQFKLEQLDLVPRGRMEDIVKGAIGLASEGFFEGVEAGRIVVHRDMTISRLLAEDGVPYAELADGTRVRADLVVCATGFTQGVPFLDRGVQDRLFDERSNFMLYRQILPLGVPGLYFNGYNSSFFSPLNAEMAAVWIAAHMVGAAPVPAPDVMRRVVVDQLAFMDEATHRHHCRGTKIIPFSMHNVDEVLDDLDLNISKRVRASHWLNVIDPAAYRGVTPALVRRLGPGRMPDADRAAREPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Z9</accession>
    <name>U5W4Z9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560630.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30165"/>
    <dbReference type="EnsemblBacteria" id="AGZ44293">
        <property type="protein sequence ID" value="AGZ44293"/>
        <property type="gene ID" value="AFR_30165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30165"/>
    <dbReference type="eggNOG" id="ENOG5034BKG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178550_0_0_11"/>
    <dbReference type="OrthoDB" id="2220755at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045645">
        <property type="entry name" value="DUF6403"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19941">
        <property type="entry name" value="DUF6403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="103" mass="10532" checksum="D2056BD66274ED47" modified="2014-01-22" version="1">MSLSWVVWAVGAGVLVAAGFAAVVVPRLRAGALSRRTGWSGARAAIGTASVSRDACPVTVTEAEQLLSRAELIVAGRGGSAAARTAAECARQADRLWREAARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W531</accession>
    <name>U5W531_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="9">ATP-PFK</shortName>
            <shortName evidence="9">Phosphofructokinase</shortName>
            <ecNumber evidence="9">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pfkA</name>
        <name evidence="11" type="ORF">AFR_30340</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560665.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30340"/>
    <dbReference type="EnsemblBacteria" id="AGZ44328">
        <property type="protein sequence ID" value="AGZ44328"/>
        <property type="gene ID" value="AFR_30340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6143"/>
    <dbReference type="eggNOG" id="COG0205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020655_7_4_11"/>
    <dbReference type="OrthoDB" id="1421302at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01981">
        <property type="entry name" value="Phosphofructokinase_II_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012004">
        <property type="entry name" value="PyroP-dep_PFK_TP0108"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000534">
        <property type="entry name" value="PPi_PFK_TP0108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="SSF53784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFK" evidence="10">
        <location>
            <begin position="78"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="205"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="250"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="356"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="151"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="176"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity; cannot use PPi as phosphoryl donor" evidence="9">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ44328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="435" mass="46613" checksum="F44571C452338A14" modified="2014-01-22" version="1">MVDPGQLIVRSLGESRIPSPLGSLLTRRGTTEHYVDEADRVLLDDTVAMLAARGDELPSFEPGGPRRRIYFDPSKIRAGIVTCGGLCPGLNDVIRGLVLTLNNQYGVSRILGFRNGYQGLTPGAGHSVVELTPAGVEYINEHGGTILGTSRGNQDPEEIVDCLEQLGVNLLFVVGGDGSMRGARLISETIAARDRRIAVVGVPKTIDNDIPYIGQSFGFQTAYAKAAESIRAAHVEAAAQPSGVGLVQLMGRHSGFIASYAALANNDADYVLIPEVPFSLDGFLPALRRRVADRGHALVVVAEGAGQEHLRDAPLRLDASGNRKLGDFGLHLRDRILDDFAAAAREVNLKYIDPSYAIRSVPANPYDSVYCLRLAQAAVHAAMAGRTELVVGMWRGRFVHVPIPLAVSGRNQVDPDGDLWLSVLEATGQPPDFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W580</accession>
    <name>U5W580_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Arginine repressor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argR</name>
        <name evidence="12" type="ORF">AFR_30220</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Regulates arginine biosynthesis genes.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-arginine biosynthesis [regulation].</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ArgR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560641.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30220"/>
    <dbReference type="EnsemblBacteria" id="AGZ44304">
        <property type="protein sequence ID" value="AGZ44304"/>
        <property type="gene ID" value="AFR_30220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6119"/>
    <dbReference type="eggNOG" id="COG1438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097103_1_1_11"/>
    <dbReference type="OrthoDB" id="1640037at2"/>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034618">
        <property type="term" value="F:arginine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00173">
        <property type="entry name" value="Arg_repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001669">
        <property type="entry name" value="Arg_repress"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020899">
        <property type="entry name" value="Arg_repress_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036251">
        <property type="entry name" value="Arg_repress_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020900">
        <property type="entry name" value="Arg_repress_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34471">
        <property type="entry name" value="PTHR34471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01316">
        <property type="entry name" value="Arg_repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02863">
        <property type="entry name" value="Arg_repressor_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01467">
        <property type="entry name" value="ARGREPRESSOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55252">
        <property type="entry name" value="SSF55252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01529">
        <property type="entry name" value="argR_whole"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Arg_repressor" evidence="10">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Arg_repressor_C" evidence="11">
        <location>
            <begin position="85"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ44304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="17318" checksum="3DAC894B26985A8D" modified="2014-01-22" version="1">MTGPGTRAARHARIVDLIRDKAVRSQTELAELLGLDGVQVTQATLSRDLEELRAVKAGGVYVIPEDGMPALRPVEQAPARLIRLLRELLNSVDVSGNLVVLRVPPGAAQFLASALDRSGLPDVVGTIAGDDTILVVARDSGDGPGAALAEKLTAWSRTDPEGSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5F6</accession>
    <name>U5W5F6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_29905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560579.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29905"/>
    <dbReference type="EnsemblBacteria" id="AGZ44242">
        <property type="protein sequence ID" value="AGZ44242"/>
        <property type="gene ID" value="AFR_29905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6057"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_0_3_11"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="21"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="188"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="376" mass="38732" checksum="04EF74746A3CE511" modified="2014-01-22" version="1">MSDAAQAWHGSVALVLGSSTGGVGQHVASLARGLLAAGCRVLVCGPAATDELFGFAASGADFAPVEISANPGPQDSGAVRALRKALTGRDLDVVHAHGLRAAFVASLARPAAPLVVTWHNAVLARGLRGQASALVERIVARAATLTLGASEDLVERAKSLGARNVRLGAVASPIAFAAPKRSRAAVRAEFRLTPDTPLIISVGRLHPQKRYDVLLEAAARWRDLTPPPVVVVAGSGPSYMLLAQRASELHAKFYLLGHRHDVPDLLAGADLAVITSDWEARQLFAQEVLLAGVPLVSTAVGGLPGLVGDAAVLIPPGDIDALDTAVRELLADPAKRADYAARGPRQAAAWPTEADTVADVLAAYASLTTAARAGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5I1</accession>
    <name>U5W5I1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363766.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24550"/>
    <dbReference type="EnsemblBacteria" id="AGZ43176">
        <property type="protein sequence ID" value="AGZ43176"/>
        <property type="gene ID" value="AFR_24550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4973"/>
    <dbReference type="eggNOG" id="COG3689">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079638_0_0_11"/>
    <dbReference type="OrthoDB" id="1936285at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015402">
        <property type="entry name" value="DUF1980"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09323">
        <property type="entry name" value="DUF1980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03943">
        <property type="entry name" value="TIGR03943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="24974" checksum="6F03478790974972" modified="2014-01-22" version="1">MNKQAQAVIMLLFGGAILKASLSDLYLRYVKEALQPFLIGAGILLVAAAVLTLWYDLRGRAATDDGHGHHHEPRVGWLLLLPVLGLLLVAPPALGSYAAGQAGTVLTAPGSDSDYPALPAGDPVDVGLLDYASRAVFDGGRSLTGRTLRLTGFVTPGPDGEPMLARMVMSCCAADGRPIKVGLDGDSRVTAAPDTWVQVTGAWTTRTAKDPVNQAEVPYLQVTGWQEITAPKQQYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6I8</accession>
    <name>U5W6I8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560950.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31785"/>
    <dbReference type="EnsemblBacteria" id="AGZ44617">
        <property type="protein sequence ID" value="AGZ44617"/>
        <property type="gene ID" value="AFR_31785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31785"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_1_11"/>
    <dbReference type="OrthoDB" id="1167152at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="23173" checksum="B6FD8D0001D97A98" modified="2014-01-22" version="1">MTVRAVVFDLDGVIIDTEEVWEEVRRGYVLEHGKAFLPDSQSRMMGMSTPEWSTHLSAEVGVPRSPEQVAADVLGRMAERYRADLPLIPGSVDAVRRLAAHFPVALASSSARILIDQVLETAGLTGTFRVTLSTEEVPRGKPAPDVYVAACEQLGFAPEACAAVEDSSNGLRSAGAAGLAVIAVPHGVYPPADDALALASLVIDGLDELTIEVVEKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6K7</accession>
    <name>U5W6K7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561185.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32950"/>
    <dbReference type="EnsemblBacteria" id="AGZ44848">
        <property type="protein sequence ID" value="AGZ44848"/>
        <property type="gene ID" value="AFR_32950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6668"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004665_5_3_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="725"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="793" mass="85907" checksum="2EB02427CB78ED1B" modified="2014-01-22" version="1">MSTATTARGSLGNLPAEVTSFVGRRQEMTGVRRMLSTSRLVTLTGAGGVGKTRLAQRAGLELRRAFPDGVWLVELAELRDPDLVAVTVAEALGVREESVSPDAPGLAGFLAGKQALLILDNCEHLVGACADLTETLLRACPRLRILVTSRQALRITGEATLTVHPLSVPEPDVDCTPADLSRYESASLLVERATAVLPGFTVTDANCSTITALCQALEGMPLAIELAVARLRVLSLDQILERLTDRYRLLTAGARNAPARQQTLRALIDWSWDLCSEQERVLWSRLSVFSGGFELDAAEQVCADDTLPAEAILDLIASLVDKSVISRTGDGARARYKMLEVVREYGAARLGGAGEHPAIARRHCDWFADLAAYGDAHWVSPDQAALMLKLRHEQANLRVALEYAVSEGPPERALRFAADLQNHWFVRGFLSEGRHWLDRALALPPPRHWTRVKALRVASWIACVQGDRDRAEALLADAKALAATLPPSPERAFIPVVEGNIAMFGGDQARALPLFEQALTGFRELHSLSGEMWTLAVLGLARGLATDDPTRGYPDLMACRDLAAASGEVWWRSFAMWALSVLRWRAGDTAGAAVAAKESLEVGKLVEDEQFGVGLSLESLGWVAGSEHRDQRAAQLLGASERMWRAMHASLSSFRSLHDFHDESMSQIRARLGDRAFDAAFRRGAELTTAEAVALALERSGRAAVPGQRVSPPAAPPAAAPEVTEARLVDLGLTRREREVVTLIAAGLSNREIAARLVVAQRTAEGHVENILSKLGFTSRAQVAGWLATQRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6L8</accession>
    <name>U5W6L8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33000"/>
    <dbReference type="EnsemblBacteria" id="AGZ44858">
        <property type="protein sequence ID" value="AGZ44858"/>
        <property type="gene ID" value="AFR_33000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6678"/>
    <dbReference type="HOGENOM" id="CLU_214515_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="48" mass="4913" checksum="7B5A4F783E3E1370" modified="2014-01-22" version="1">MGIQGILYLIAVILLVLAALPIGTRGISLALLGAAFALLAFSWDTITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6P4</accession>
    <name>U5W6P4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integral membrane sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_32830</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32830"/>
    <dbReference type="EnsemblBacteria" id="AGZ44824">
        <property type="protein sequence ID" value="AGZ44824"/>
        <property type="gene ID" value="AFR_32830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6644"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="3">
        <location>
            <begin position="183"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="431" mass="45381" checksum="5F3810E005C7D05C" modified="2014-01-22" version="1">MVVVGWMGRLFDARDTFVRMILLDLSGVSYLLFGTEGHPPTRNQLILAVLAFASALIFHRQRVVNLVVQTALLVVAFWLLDDTMINQVGASWALLELAMWAPRVRFLVAGAGLVSAVYLAFTLGDQPFRLTTPIYGLFVVVGVPALIGQVIRTTRELGLEAQHRALEEQRRRESETRAARADERSAIARELHDVVAHHVASMVLRVGVAKHVLPDTDPRVTEVFDDVHSTGTAALADLRRLVAVLRDPSVRTDAALTVIDPGALPAALDAAVETARRAGLTVDAAISPEVGTLDAVRGLAILRLTQEGLTNVAKHAGTAAHARLRVAVLDGDVLWSIADDGGGTSPGLPSGGGHGLTGMRERVEVLGGHLTVGPAAGGWRVETVLPATQPPPDLGPSTGPANSLPADPPTSVPSGDEPLVDRASGPAAGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6X6</accession>
    <name>U5W6X6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561236.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33205"/>
    <dbReference type="EnsemblBacteria" id="AGZ44899">
        <property type="protein sequence ID" value="AGZ44899"/>
        <property type="gene ID" value="AFR_33205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6720"/>
    <dbReference type="eggNOG" id="ENOG5032Y91">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097052_0_0_11"/>
    <dbReference type="OrthoDB" id="2009377at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="25054" checksum="FBB1D132F59410F2" modified="2014-01-22" version="1">MSEQAPDRIWSGIEITKVIAGTLAAVTAAVIGSFLGVAGTLAGAAVASVVGSVGTEIYQRSLNRGAKRLGSIAPTFVKVPAAVGTPAVAAATEEESPSHTVAPKKNIRWGHVALAATTLFVLAMGSLTLFELFSGKSVASTVGTDSSGRTSVSSIFGDDSGDKQKPAVTPSTGPSGETTTDSPDAPASGAPTSTAEPTATPTTTDPAAPPEPTTAPTTDAPQQTPPTDTQNQDGEPLQQQNPDQGSDTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W707</accession>
    <name>U5W707_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560054.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27275"/>
    <dbReference type="EnsemblBacteria" id="AGZ43716">
        <property type="protein sequence ID" value="AGZ43716"/>
        <property type="gene ID" value="AFR_27275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5527"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_31_0_11"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="158" mass="17368" checksum="8FC5AA086B043484" modified="2014-01-22" version="1">MADIRLRPVRDEDLDTFYDQMRDPEAVQVAAFTAEDPDDRSEFDAHMARVLGRPDVTMRAITVDGVLAGHIASFEVGGDTEITYWLGREWWGRGIATHALGLLLELVTVRPITARAAGDNAGSLAVLRKTGFRPIGTEIAYATGRGKEIEETVLRLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W765</accession>
    <name>U5W765_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PKD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560119.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27600"/>
    <dbReference type="EnsemblBacteria" id="AGZ43781">
        <property type="protein sequence ID" value="AGZ43781"/>
        <property type="gene ID" value="AFR_27600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5594"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002470_0_1_11"/>
    <dbReference type="OrthoDB" id="1046649at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666398" description="PKD domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="2">
        <location>
            <begin position="501"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="692" mass="73804" checksum="E20BA758F952B32D" modified="2014-01-22" version="1">MKRLLTAVPVLALVATALSAPSGPATAQAAAAPPPDSAFQKVTLNDFPGEPIALAVLPDKRVLHTSRKGEVRINDPKSGRNILAATIPVYLHDEEGVQGVAIDGNFAQNKWVYIYYSPPMNTPSDDPATPGVNEGDAPETGTAADFARYKGVMRLSRFKLNGNTLSLGSEQKIMEVGTDRGMCCHVGGKIDFDNAGNLYLSTGDDTNPFASGGYVPIDERANRNPAFDAQRTAGNTNDLRGKLLRIKVNNNGSYSIPKGNLFKPGTAYTRPEIYAMGLRNPFRFAVDKSTGVAYVGDYSPDANSPNPARGPAGHGRWMAIDKPANYGWPYCVSPELPYVDYDFATGTSGAPFNCAAPVNESPNNTGKRKLPPVEKAEVIYSYAASAEFPELGTGGIGPMGGPAYEFDAKNKSATKWPQYYDGKPLFYEWTRDYVKGFNLGPANEVTSIESVLPSIVFDNPMDLEFGPDGALYVLEYGDGYFSENPEAQLARIDYVRGNRTPIPRVSATPTSGEAPLTVTFSSAGTTDPDGDSLKYAWDFNGDGVVDSRQANAVYTFTENGNYRPTLKVTDPTGRWASAEVLLPVGTLAPVVTLVTPTDGQPFEFGDVVPFEVSVVDDQPVDCSKVTVTYILGHAEHGHPLSTASGCSGSITTFLDAGHAGADDLTGVFVAQYTDSGTPPQSGSDQVVLTPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7U0</accession>
    <name>U5W7U0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28800"/>
    <dbReference type="EnsemblBacteria" id="AGZ44021">
        <property type="protein sequence ID" value="AGZ44021"/>
        <property type="gene ID" value="AFR_28800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5838"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_11_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="81"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16111" checksum="21A578A3272EED78" modified="2014-01-22" version="1">MRSLHLHDDATGSRPRGGTDEYVARALEAGAAGFLGKDVTPEILLAGIRTVVGGDALLSPSATRSLINRFLSGPGSVDDLSLKSLAVLTDREREITALAAHGLSNQDIAQRLVVSPLTVRTHIHRAMTKVGARDRAQLVVIAYQQGLVRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W876</accession>
    <name>U5W876_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560458.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29300"/>
    <dbReference type="EnsemblBacteria" id="AGZ44121">
        <property type="protein sequence ID" value="AGZ44121"/>
        <property type="gene ID" value="AFR_29300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5937"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025711_1_1_11"/>
    <dbReference type="OrthoDB" id="948454at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="228" mass="24212" checksum="A7D33D9127BE7954" modified="2014-01-22" version="1">MKIFIIGITGGVGGQVARKLREQGDDVAGLVRREEQKGELAEFGAVGHVGDLTNGSARALADMIGDAETIVFSAGAGGGSREAIAAIDRDGVIKAIEAAHLAKVQRFVLVSVFPEAWRDRFEGDGFEYYIRMKKQADVALSRSGLDWVILRPAALQDYSGRGTIALGPAEIHEQVSREDVATTLAAVVHETGISRQILELNAGDTPVSEAIRAAVRDTTRAGEDSRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8B4</accession>
    <name>U5W8B4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_35640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35640"/>
    <dbReference type="EnsemblBacteria" id="AGZ45384">
        <property type="protein sequence ID" value="AGZ45384"/>
        <property type="gene ID" value="AFR_35640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7212"/>
    <dbReference type="eggNOG" id="COG5164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663300_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="488" mass="48141" checksum="62179FFE47DA0CE2" modified="2014-01-22" version="1">MSPATPVPHYAAAGPSPTPPEPAPGDQVGTAAGDNAENNNVTSQQYGYGPFHGSPQQPSDTHPAATPNAPTSGTPPVSAAGPGHETSQPGPGHTNPTSGAGPGYGESPTSGVGPGHGKTPVSGPGFGGGNPTSGAGLGYGNTPTSGAGPGYGNTPVSSAGPGFGADNPTSGAGPGYGNTPVSSAGPGFGADNPTSGAGPGYGNTPTSSAGPGYGGDNPTSGAGPAYGENYSTSSPPSWSSTDPISGSGAAADPYGFSVAPAFGGGPRIEPTPKQPKGRYLIPALAGLVAGLLIFGTGGFFAGRATGGTDTPAPPAPPAPTATAPAGLGPYEQNQVALNQPKLTGSLAVISQGWLPNLSGCSRSGEKGGPVLNKGEKVRVRCEMDAMSAIFVEYATDADRDKARVKTLGQNVDARKLTPGVGPATERATPSGRTNGNYVEFAYPASGKTVSGIWWDDAATPVAAYLLAYWKEGTGSQWEPMRDIWARYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8I4</accession>
    <name>U5W8I4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6 7">50S ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rplS</name>
        <name evidence="8" type="ORF">AFR_36310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6 7">This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561854.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36310"/>
    <dbReference type="EnsemblBacteria" id="AGZ45518">
        <property type="protein sequence ID" value="AGZ45518"/>
        <property type="gene ID" value="AFR_36310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7348"/>
    <dbReference type="eggNOG" id="COG0335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103507_2_2_11"/>
    <dbReference type="OrthoDB" id="1698718at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00402">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="L19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_L19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018257">
        <property type="entry name" value="Ribosomal_L19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="PTHR15680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002191">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01024">
        <property type="entry name" value="rplS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01015">
        <property type="entry name" value="RIBOSOMAL_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="119" mass="13612" checksum="3F34CB25973718B7" modified="2014-01-22" version="1">MNTLDALDAQSRRTDIPDFRAGDTVKVHVRVVEGNRSRVQVFQGVVISRQGGGLRETFKVRKISFGVGVERTYPINSPQLDRIEVVTRGDVRRAKLYYLRELRGKKAKIKELREKQTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8P4</accession>
    <name>U5W8P4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_30500</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560697.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30500"/>
    <dbReference type="EnsemblBacteria" id="AGZ44360">
        <property type="protein sequence ID" value="AGZ44360"/>
        <property type="gene ID" value="AFR_30500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6175"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012253_0_0_11"/>
    <dbReference type="OrthoDB" id="567019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665807" evidence="2">
        <location>
            <begin position="33"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="GSDH" evidence="3">
        <location>
            <begin position="63"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="368" mass="38868" checksum="5231820F239AB31F" modified="2014-01-22" version="1">MAEILSGRRWFRTAAAVLTVGVLAACDSGSEASPGVIATSTASSAPKGAPDLGAGEELAHGIDVPWGMTFLPDGDALIAERDSARVLRLKPGSGEAREVYKVPGVVASGEGGLLGLAVSPKFAEDNLVYAYFTAENDNRIARFELDGDGAPEVIFDGIAKATYHNGGRIAFGPDGMLYAGTGDAGEKPRSQDPASPNGKILRLTPDGKPASGDSPVYSLGHRNVQGLAWDSSGRMFATEFGQNELDEVNLIQQGRNYGWPEVEGEGDTDGGRYTNPLVTWATKDASPSGMAISGTTAYVAALRGQRLWTIPLDGEKAGEPKAEFKEKYGRLRTVQIAPDGALWLTTSNTDRGKKLHEGDDRVLRFPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8W2</accession>
    <name>U5W8W2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561890.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36490"/>
    <dbReference type="EnsemblBacteria" id="AGZ45554">
        <property type="protein sequence ID" value="AGZ45554"/>
        <property type="gene ID" value="AFR_36490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7383"/>
    <dbReference type="eggNOG" id="ENOG5033G9I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182347_1_0_11"/>
    <dbReference type="OrthoDB" id="2079019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="64" mass="7088" checksum="C9D06D407E7E94C2" modified="2014-01-22" version="1">MAKALYGHVGAAPDRRLLDEVTRLRSRVQALEFEITRLRAENDRLAAAAAEADDLLRLSEPALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8W4</accession>
    <name>U5W8W4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Formamidopyrimidine-DNA glycosylase</fullName>
            <shortName evidence="15">Fapy-DNA glycosylase</shortName>
            <ecNumber evidence="15">3.2.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">DNA-(apurinic or apyrimidinic site) lyase MutM</fullName>
            <shortName evidence="15">AP lyase MutM</shortName>
            <ecNumber evidence="15">4.2.99.18</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mutM</name>
        <name evidence="15" type="synonym">fpg</name>
        <name evidence="19" type="ORF">AFR_36515</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.</text>
            <dbReference type="EC" id="3.2.2.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the FPG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.23" evidence="15"/>
    <dbReference type="EC" id="4.2.99.18" evidence="15"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561895.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36515"/>
    <dbReference type="EnsemblBacteria" id="AGZ45559">
        <property type="protein sequence ID" value="AGZ45559"/>
        <property type="gene ID" value="AFR_36515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7387"/>
    <dbReference type="eggNOG" id="COG0266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038423_1_2_11"/>
    <dbReference type="OrthoDB" id="1162346at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008534">
        <property type="term" value="F:oxidized purine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00103">
        <property type="entry name" value="Fapy_DNA_glycosyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="DNA_glyclase/AP_lyase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020629">
        <property type="entry name" value="Formamido-pyr_DNA_Glyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="MutM-like_N-ter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000214">
        <property type="entry name" value="Znf_DNA_glyclase/AP_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="SSF81624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00577">
        <property type="entry name" value="fpg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51066">
        <property type="entry name" value="ZF_FPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13 15 19" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 15 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FPG_CAT" evidence="18">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="FPG-type" evidence="17">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DNA" evidence="15">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="3"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for beta-elimination activity" evidence="15">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for delta-elimination activity" evidence="15">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AGZ45559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="31721" checksum="B2ABACA7ED2135E3" modified="2014-01-22" version="1">MPELPEVETVREGLAKWVTGRRISTVEVRHPRAIRRHVPGDVHFAAILAGRSITGVARRGKYLWLPLDSGDAIIAHLGMSGQLLMQPAEAEDELHLRVRFTFDDNGPQLRFVDQRTFGGLSVSEDGAELPAEIAHIARDPMDPLFDDEQFVAALRRKHTEVKRALLDQTLISGIGNIYADEALWRAQLHGARATDALTKPAVRRLLGHVRDVLGEAIVAGGTSFDALYVNVNGQSGYFDRSLNAYGREGQPCHRCGTIMRREQFMNRSSYSCPRCQPRPRQPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W902</accession>
    <name>U5W902_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44475">
        <property type="protein sequence ID" value="AGZ44475"/>
        <property type="gene ID" value="AFR_31075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6290"/>
    <dbReference type="eggNOG" id="ENOG50327XX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876125_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="34983" checksum="57CBE9CF710BD95C" modified="2014-01-22" version="1">MEDEPPADFIAFVAGRLGALRDEAARLVGGERFAAEVYMEVLTDLAGHWRRVCWRSRLSHRDAATEYLNRRLHTRSRQWREDQIYPVEVTVSHHDPWSTPIDGSQISLDEMTVSHRGRSSTPDDGARIFLDPEPLRPTPVETARYARPRTDPAQPPGPPPSHTSDHHISTVVRMPAPVQPSGPPTPSTADPAGTVEASPQERSLTPTAWGQANLVEAATAQGRQHPGEATTRRPLTAAAAAAQWQGHSVNDETVAQRLAPLLPSTARAEVRVVAEAEIAWVHAYRRYVWRRYARLGGTFVLVLGSFVQFMSQVSAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W977</accession>
    <name>U5W977_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Neutral metalloproteinase</fullName>
            <ecNumber evidence="8">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_37175</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Extracellular zinc metalloprotease.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase M4 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37175"/>
    <dbReference type="MEROPS" id="M04.017"/>
    <dbReference type="EnsemblBacteria" id="AGZ45689">
        <property type="protein sequence ID" value="AGZ45689"/>
        <property type="gene ID" value="AFR_37175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7521"/>
    <dbReference type="eggNOG" id="COG3227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008590_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011096">
        <property type="entry name" value="FTP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023612">
        <property type="entry name" value="Peptidase_M4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001570">
        <property type="entry name" value="Peptidase_M4_C_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013856">
        <property type="entry name" value="Peptidase_M4_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07504">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01447">
        <property type="entry name" value="Peptidase_M4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02868">
        <property type="entry name" value="Peptidase_M4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00730">
        <property type="entry name" value="THERMOLYSIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023039911" description="Neutral metalloproteinase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="FTP" evidence="11">
        <location>
            <begin position="48"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M4" evidence="9">
        <location>
            <begin position="187"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M4_C" evidence="10">
        <location>
            <begin position="336"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623612-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ45689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="514" mass="52728" checksum="28ABCC2C7A5AE9A3" modified="2014-01-22" version="1">MKRSLAAVGSALLMGGLLTGVGATSAAAAPDPLSPSAHRAALKASAADAFAVKATAKDADGTTHVRYTRTYEGLRVLGGDLVMHGTKDGAFRQASVGLAAPLTLSTTPKRAASTASATAKAHFTGTVSAVGAPELVVDATSGTGTLAYETVVEGFGEDGQTPSKLHVVTDATTGAVLSSWDTVETATGSGRSVYSGTVSLTTTQVSTTSFSLKDASHGNGTTCDMNNRTSGTCTLFTDADNTWGTGATSNRQSAAVDAHYGAAKTYDYYNTTFGRKGIFNNGTGVPSRVHYGSAYVNAFWDGSSMTYGDGSGNARPLVSLDVAGHEMSHGVTERTANLTYSGESGGLNEATSDIFGTMVEFYAANSSDAGDYLIGEKININGNGAPLRYMYNPTLDGKGIGCWSTSTKNVDVHYSSGVGNHFFFNLAEGTGATAYGTSPVCSGAGAVTGIGRSKAAAIWYRALSTYFTSSTNYKAARGYTLSAATDLYGKCGTEYKAVQKAWTAVNVTGTDAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9K0</accession>
    <name>U5W9K0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_31950</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560983.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31950"/>
    <dbReference type="EnsemblBacteria" id="AGZ44650">
        <property type="protein sequence ID" value="AGZ44650"/>
        <property type="gene ID" value="AFR_31950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6467"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_8_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="105"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="331"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="361" mass="38510" checksum="A1E74AAED023CA7B" modified="2014-01-22" version="1">MTIPTSSIDQVIPGQGAMAQPEPAKPKKEKRRRFRFVSNRKAATGIIVLGIYLLFAIIGPWVAPYDPDARGDQFVAPPSADHWFGTTHLGQDIFSQILVGTRAVMLVGLAAGVLATLLSVLIGVTAGYLGGGWDDSLSALSNVFLVIPALPLIIIVTAAVPGGGDTLVALIIGFTSWAWGARVLRAQTLSLRRRDYIEASRANGESTWRIVIFEILPNLTAIIASGFVGTVIFAVTSEITLAFIGIGSVSSWNWGTILFWAQSQQALAQGAWWWFVPAGLAIAFLGTALSLINFGIDEFVSPRLRSMGKTKVKTASGRTVRMRVGFTPVLDNSAAPHTHASPIPVIGGKPTPVNRSKEAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9K6</accession>
    <name>U5W9K6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="1">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_38195</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562228.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38195"/>
    <dbReference type="EnsemblBacteria" id="AGZ45893">
        <property type="protein sequence ID" value="AGZ45893"/>
        <property type="gene ID" value="AFR_38195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7727"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_6_11"/>
    <dbReference type="OrthoDB" id="652304at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665693" description="Peptidoglycan glycosyltransferase" evidence="9">
        <location>
            <begin position="30"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="11">
        <location>
            <begin position="73"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="10">
        <location>
            <begin position="364"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="735"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="767"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ45893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="786" mass="84320" checksum="6377E1B1C6BEF662" modified="2014-01-22" version="1">MRGRDHHFVTNVASLLICGLLAGVAVAAAAFPAVAMSGLAAKAGSEGFASLPSEIKHATAPQLTRVFASDNKTPIAVFFDEFRSDVPLKDISINMRNAIVAAEDHEFFTHNGVDLKGAARALVNNTGKDNNKQGASTLTMQLVRMSLSYSASTPTEVVNATKDTPTRKISEMKYAMQLEKELTKDQILGRYLNAAPFGNGAYGVFAASQVYFKKSPKNLTVPEAALLAGMVKAPTGFDPTTAIGYPKALDRRNWVIGNMRDLNYITPAQADTAIKVKLGKTATRTGNGCAAVKVNHWGFFCDYFYRWWLSRPEFGQNVYERERRLKSGGYRIQSSLDVKAQKATRENISDRIGINNRDALLLAAVEPGTGRVRALAANRRFKLDDGKNKLSSDPAKRAKKIRGSYPNTTNPLLSGGGDITGYQAGSVFKMFTMVAALENDVPLAYQINTTYKYTSKLYRDSPGCGGFYCPTNASASEKGPYNMWTGFGSSVNTYFVPLAEQIGAEKVVDVAQRFGVQFRAPQDKDLATNYAHDWGSFTLGVSASTPLDMANAYATLAGDGKYCKPTPVQQITSMNGEKLDVGKPSCRQATKPDVARAAIDAARCPIGDSAQLGSCSGRTAGGARGIVGHPIFGKTGTTDNDKTASLIIGTTSMVVAGYLVNPDYQNHPYRLSHDIVNPAVEYTMRDLMKGKEKVQFKRPSGNKIAQGEQRSIPDVNCISVAEATSRIESAGFTATPGTEVTSDCPAGQAAGTEPKNRTIKGGYVSIQISKGKEPGPGGSAPPATPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9N5</accession>
    <name>U5W9N5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="7">TC-AMP synthase</shortName>
            <ecNumber evidence="4 7">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_37390</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + hydrogencarbonate + L-threonine = diphosphate + H2O + L-threonylcarbamoyladenylate</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="4 7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37390"/>
    <dbReference type="EnsemblBacteria" id="AGZ45732">
        <property type="protein sequence ID" value="AGZ45732"/>
        <property type="gene ID" value="AFR_37390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37390"/>
    <dbReference type="eggNOG" id="COG0009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="9">
        <location>
            <begin position="8"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="320" mass="33689" checksum="0AEB1D1F6C474844" modified="2014-01-22" version="1">MSIVAPDADGLRRAVDLLHQDCVVAFPTETVYGLGAHAFSPKAVAEVYRLKNRPSWNPLIVHVSGVVAARALAEEWPDLANDLAAQFWPGPLTLVVKRAGHLAGVGAETGTIAIRVPAHDVALKLLDASGLPLAAPSANRSEGISPTTAEHVLRSLPDVPLVLDGGPASWGIESTVVDLTGETPKLLRPGALGLRALREITGTIALPDEVTDGAARSSPGMSRRHYAPKATVILAKDVSEVEHQGLAGPVAVLSYESVEDFADGLVAEVLSVDPREYAADLYAALHRLDDAGVATIIVQAPPESEEWLAVRDRLRRAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9T0</accession>
    <name>U5W9T0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45899">
        <property type="protein sequence ID" value="AGZ45899"/>
        <property type="gene ID" value="AFR_38225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7733"/>
    <dbReference type="eggNOG" id="ENOG503260Q">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1999022_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="130" mass="13956" checksum="6709CA589268A1D0" modified="2014-01-22" version="1">MVSGTGTSALAEIIGGRPVRAAGPPAADDMAAILAAAQHRAGPPLKIRRHASSDDGPAYWRWDCDSCGEYGGGRHHSDAVGRAMRHCGEHPEHRSSLLPPSRCRERDHRLAVHPMLFAMDDDPPPEPLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA32</accession>
    <name>U5WA32_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="7">EPSP synthase</shortName>
            <shortName evidence="7">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroA</name>
        <name evidence="9" type="ORF">AFR_38140</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38140"/>
    <dbReference type="EnsemblBacteria" id="AGZ45882">
        <property type="protein sequence ID" value="AGZ45882"/>
        <property type="gene ID" value="AFR_38140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7716"/>
    <dbReference type="eggNOG" id="COG0128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024321_0_0_11"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="14"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="27"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="7">
        <location>
            <begin position="94"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="170"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="427" mass="44074" checksum="9CA7DEE932E87C33" modified="2014-01-22" version="1">MTAQARPWTAPVATTPVSAGVRLPGSKSMTARALVLSALADGPSVVRRPLRARDTTLMAAGLRALGVAIDTTSDEEWRVEPGPLRGPARVDVGLAGTIMRFAPPVAALADGTVTFDGDPHARNRPLRPIVEALRTLGVVIDASPTGGLPLVVRGTGLVEGGEAVIDASGSSQFVSGLLLSAPRFAKGLVLRHEGPPVPSAPHLRMTTHMLRAAGASVDESVPDVWAVEPGALHGRTWDIEPDLSGAAPFFAAAMVTGGTVTLAGWPRESWQPVGRLTELLTALGAEVTQDDNGLTVRGTGALHGINADLSEVSELTPVVAALAALADSPSELRGVAHIRGHETDRLAALAAELGKVGAGIVEHPDGLEITPGPLRPAVFETYADHRMAHAAAVIGLAVEGVQLTDVACTSKTLPEFPELWADLVTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAH1</accession>
    <name>U5WAH1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_39670</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39670"/>
    <dbReference type="EnsemblBacteria" id="AGZ46188">
        <property type="protein sequence ID" value="AGZ46188"/>
        <property type="gene ID" value="AFR_39670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8026"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="95"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="34742" checksum="57ABB72E51E5ECC8" modified="2014-01-22" version="1">MVEQTVRAESATAAPEPAPAGPVKRRGPGATPYLFLLPYLLLFVTFTLVPAVYGLWVSLHEWDYLLPGKPFVGLDNYTALFDSASPVFDFFWQSMEASGIFTVFSVPLLVVVPLGIALLLNRSFKGRTFFRAVYFAPYVLGVAVIGLLWRFLLDPNLGAVNSLLGAIGLPDDTAWTTGLPWAWISLVGVTVWWTLGFNAVIYLAGLQDIPRELYDAAKVDGGGRWAEFRHVTLPGLRPVLLFVVINTVLASSNMFGQSYLITQGAPGVETRTAIMYIAEEGLRGYRMGSASAMSYLLTLALLIISMTIFRLFRTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAM0</accession>
    <name>U5WAM0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562507.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39600"/>
    <dbReference type="EnsemblBacteria" id="AGZ46174">
        <property type="protein sequence ID" value="AGZ46174"/>
        <property type="gene ID" value="AFR_39600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8011"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045983_2_0_11"/>
    <dbReference type="OrthoDB" id="1086535at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666241" evidence="2">
        <location>
            <begin position="30"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="324" mass="32095" checksum="0D08287C7659B271" modified="2014-01-22" version="1">MSRLALLIAVAVVALQALLVPLFAGPAANLEPRDLPIAVAGPAPAADQVAAQLAAAHPGAFDVRVVTDPDAAIRNRDVYGAIVLGAQGPSLHLASAASPAVATLLTQATAGLGQGRPVPVVDVVPTDADDPRGAGFGAGFLPLAMTGMIAGILTFLLVKRRGARLLALGSFSVLAGLGGAAVQQLWLGVLPGDYLSNAAVMGLFGLAVSGTVAGLAAVLDRAGLALGALIVFLVGNALSGVSSAAELLPQPWGAVGQFLPIGAGSTLLRSVAYFDGNGAAVAWWVLSAYAIGGLVLVSAGRRGLSRPAAEVAPGAVEREPALVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAS6</accession>
    <name>U5WAS6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39975"/>
    <dbReference type="EnsemblBacteria" id="AGZ46249">
        <property type="protein sequence ID" value="AGZ46249"/>
        <property type="gene ID" value="AFR_39975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8088"/>
    <dbReference type="eggNOG" id="ENOG5033M2G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186189_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="52" mass="5600" checksum="F5AEA279DC4229B6" modified="2014-01-22" version="1">MIKKVLTWLGIAFLIFFIAFNPNSAAAVFESLGSTIGDIARGFGTFFTNLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB26</accession>
    <name>U5WB26_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ATP-dependent helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_40820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562750.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40820"/>
    <dbReference type="EnsemblBacteria" id="AGZ46418">
        <property type="protein sequence ID" value="AGZ46418"/>
        <property type="gene ID" value="AFR_40820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8264"/>
    <dbReference type="eggNOG" id="COG1199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012117_2_0_11"/>
    <dbReference type="OrthoDB" id="679382at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="PTHR11472"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="29"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="684" mass="72330" checksum="46DC4AB79A3EB66A" modified="2014-01-22" version="1">MVSSVVAGTVSDVTAATRKKATASQLLAAAVGAVPGGASRPGQEQMATAIERAVKEREHLLVQAGTGTGKSLAYLTPSLLVDGPVVVSTATLALQNQLVEHDLPRLADAVEPVLGRRPTFAVLKGRHHYLCVAKLEHADEEAPTDTLFDDAPPRATQWLGEAGRLGKQILRVQEWAEKTKTGDRDELDPGVDETAWRQVSMPARDCPGVAKCPYGAECFAEASRVRAREADIVVTNHSLLAVDMLADRNIVPPHKLLIVDEAHELADRVSSAAQAELTPEGVERASKRARPLVSPAAAEALAEAADSLTVGLSDIPAGRLTTGLPPLIHQAVMLIESATRSALDAIGDIKNDDPDPVRKQQAKATLDELSKTSQRLLEDSDFDVAWVEKSDLGNGRRALVVAPLSVAGTLASSLYDERTVVATSATLTLGGRFDTVARSLGLPTAAPVPAARSAEAPPISGDGWSSLDVGSPFDYPRQGILYVASHLPRPAASGLPDAAGEELLKLVEALGGRTLGLFSSRKAATQAAELLRARTDLPVLLQGEENLPILVRKFREQKESCLFGVMSLWQGVDVPGDACQLVVIDRLPFPRPDEPLAAARAAAVDATGGSGFSAVSVPIAAIRLAQGVGRLIRSTGDKGVVAVLDSRLETARGYGPFLRKSLPPFWYTTRPEVAIGALQRLSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC65</accession>
    <name>U5WC65_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RelA_SpoT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42795"/>
    <dbReference type="EnsemblBacteria" id="AGZ46813">
        <property type="protein sequence ID" value="AGZ46813"/>
        <property type="gene ID" value="AFR_42795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8657"/>
    <dbReference type="eggNOG" id="COG2357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1033016_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015969">
        <property type="term" value="P:guanosine tetraphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RelA_SpoT" evidence="1">
        <location>
            <begin position="112"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="29676" checksum="60737F9D26022E66" modified="2014-01-22" version="1">MELLVEVARRAVITEFGTLEQFLSGHPGVGDLFEPVEHQVNTTPDGLSRKKAFEAEARRRDPVRGVGPAPTLAELTLLDDYGIRLRRRVLPAATLEVEELASQLGGDALVSGRSKRSGDLLDKVRRMTSERPNRPARLDYRVGDVIDAVGLRLTVPDMDTLSAALELVKTHFGDRILEIENMYAQPKAQAPSYRVIPVIISIETDGLPCTYELQLTTQRASVAADIEHNTIYKPYVAVNGLQREAVEAAMEEAAALDQLETIERDGHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCL7</accession>
    <name>U5WCL7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562002.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37060"/>
    <dbReference type="EnsemblBacteria" id="AGZ45666">
        <property type="protein sequence ID" value="AGZ45666"/>
        <property type="gene ID" value="AFR_37060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7498"/>
    <dbReference type="HOGENOM" id="CLU_1472226_0_0_11"/>
    <dbReference type="OrthoDB" id="2226291at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="18760" checksum="33CF0523F3E4E24F" modified="2014-01-22" version="1">MTMPGPRVTPVRRTVGVAVALVTGQALLGGIIGFVTFGGESDTAPGTGTAAPQIAGPPIVLPPASLPPSGRSAAPKKPATRKSSATTRPASRRPTPAPERRSAEPSATTARPATPSPTPRPTKTTTPPGLVLLPPVPADDEGSEDPVVVRERCDDEGATGRTSDGKVVRCVRGRDGDLRWQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD34</accession>
    <name>U5WD34_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Poly(ethylene terephthalate) hydrolase</fullName>
            <ecNumber evidence="7">3.1.1.101</ecNumber>
            <ecNumber evidence="3">3.1.1.74</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_38010</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(ethylene terephthalate)(n) + H2O = (ethylene terephthalate)(n-1) + 4-[(2-hydroxyethoxy)carbonyl]benzoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49528"/>
            <dbReference type="Rhea" id="RHEA-COMP:12420"/>
            <dbReference type="Rhea" id="RHEA-COMP:12421"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:131701"/>
            <dbReference type="ChEBI" id="CHEBI:131704"/>
            <dbReference type="EC" id="3.1.1.101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:49529"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47348"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:50477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:47349"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.101" evidence="7"/>
    <dbReference type="EC" id="3.1.1.74" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562191.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38010"/>
    <dbReference type="ESTHER" id="9actn-u5wd34">
        <property type="family name" value="Chlorophyllase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45856">
        <property type="protein sequence ID" value="AGZ45856"/>
        <property type="gene ID" value="AFR_38010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7689"/>
    <dbReference type="eggNOG" id="COG4188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050214_0_0_11"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041127">
        <property type="entry name" value="Chlorophyllase2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12740">
        <property type="entry name" value="Chlorophyllase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666287" description="Poly(ethylene terephthalate) hydrolase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="18"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ45856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32264" checksum="9A5C982DD26E2F62" modified="2014-01-22" version="1">MGRRLAALIVAVAALTACSAPQDEQPEQPQRPPSQEAGRTVAPQPKPTKPSPAAGAPSRTYPVEVRKLQLSRGKDRPLPTTVWYPETGKGPFPVIVFSHGLTAQPSDYGRFLTRWARAGFVVAAPAYPHTSTGVADFDVIDLINQPADASYVLTKILALDAKSGDALKGRLDTGHVAAAGHSGGGITTIGMLSGNRDTRLSAAVVLAGRQVLPVPFSGPATPVLFVHGKLDRTVRYSEGLAAFDAVPWPKALLTIPDGGHVTTSGKDFDLVATATTDFWRWTLYGDTTAKQRLERVKNLDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD88</accession>
    <name>U5WD88_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562251.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38310"/>
    <dbReference type="MEROPS" id="S16.012"/>
    <dbReference type="EnsemblBacteria" id="AGZ45916">
        <property type="protein sequence ID" value="AGZ45916"/>
        <property type="gene ID" value="AFR_38310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7750"/>
    <dbReference type="eggNOG" id="COG3480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042037_1_0_11"/>
    <dbReference type="OrthoDB" id="1324470at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666288" evidence="1">
        <location>
            <begin position="25"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="129"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="342" mass="35561" checksum="AF421E76EC935D9F" modified="2014-01-22" version="1">MRRRGVTVILGALITALLAVGVMAFPLPYVVLKPGPTVNTLGSDNGKEVIQVTKADTSTSQGQLRLTTVGVQPSVELVWAIRGWFSDEQAVVPRELIYPPDRSEQQVEEQNAEEFKSSQYSAVTVALRELGYPVQTFVTEVTAGGASVGQLQKDDVITSVDGVEVTSPGQLTQLIQAKPAGTALTVGYTRAGKPATAKITTRSDSKSATPRIGVGIDKKQPHPFTVEIDLDEIGGPSAGLMFTLGIIDKLKPEDLTGGKIIAGTGTIDDEGNVGPIGGIPQKLVGAKDAGAQLFLVPADNCAEAMKNAVSGLPMAEVATADDALTALKTFTSGGTPKPCPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDT9</accession>
    <name>U5WDT9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562410.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39110"/>
    <dbReference type="EnsemblBacteria" id="AGZ46076">
        <property type="protein sequence ID" value="AGZ46076"/>
        <property type="gene ID" value="AFR_39110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7915"/>
    <dbReference type="eggNOG" id="ENOG50347YJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168327_0_0_11"/>
    <dbReference type="OrthoDB" id="1906285at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="10356" checksum="850BBEEFF5915FBA" modified="2014-01-22" version="1">MGETVYNIGEGPATRVSLSLPEGTAEAIRARVGKREFSAFIAEAVERELRGQVLDEYLADYESRKGPISEQARHGARKVFDEVFAEEGQWPAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFB4</accession>
    <name>U5WFB4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4352 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_41735</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562932.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41735"/>
    <dbReference type="EnsemblBacteria" id="AGZ46601">
        <property type="protein sequence ID" value="AGZ46601"/>
        <property type="gene ID" value="AFR_41735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8446"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072584_1_0_11"/>
    <dbReference type="OrthoDB" id="1727679at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029051">
        <property type="entry name" value="DUF4352"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029050">
        <property type="entry name" value="Immunoprotect_excell_Ig-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11611">
        <property type="entry name" value="DUF4352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4352" evidence="4">
        <location>
            <begin position="116"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="24844" checksum="BCE199CF94A22EA1" modified="2014-01-22" version="1">MSYPPQDPHQPQQPYQPPAAPGYGPPPPGYPAQPPVAAAKKGKGKLIIGIVGGLLGLCCVGGVIAAAVNGDGAADSLGEAAAAEPAAPKSTAAKAAPAAKATSKSAPPAKKPATPGLGDPVRDGKFQFTVTKLDCSKSKVGSQYLNKKAQGKFCLVSVTVKNIGKEAQLFSGSSQKALDAEGTEYSNDGAAEIYANEDSATFLNEINPGNSSKGKLIFDVPKTTKLTTLELHDSPFSGGVKVSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPL9</accession>
    <name>U5VPL9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative diguanylate cyclase/phosphodiesterase with PAS sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_02300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357692.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02300"/>
    <dbReference type="EnsemblBacteria" id="AGZ38749">
        <property type="protein sequence ID" value="AGZ38749"/>
        <property type="gene ID" value="AFR_02300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.465"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="332"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="488"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="3">
        <location>
            <begin position="629"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="892" mass="93982" checksum="004B625F223EF486" modified="2014-01-22" version="1">MDHAPRTSAWDRRLLLISGSLAVAGTVWFTVVYAFGEIGPTILGWAMMPVSAAVATVVCRRSSTDPSLTDAARLFWRRFGFAFAVLTAAMTSHVIDSIGPGLAMSTRVGAVTGLLHAAFLLLLSWPLFGLPLGARTRGQLVTTGLDVGILVSGAAVFFWHFSAQRLFEGSAAGTVSIVASVSFVITGLVTMLAIAKVAATGATSLGHRSMWALGSSLVATGLVTALTPLLATRPHLDITLLGVPVAAFLVAFGAAGTRAHGLPPAPNRRRTSPLPYVMAAATNALLIYVVAVDSSDRLPVTVVAVLLTGLVIIRQLIALRDNDNLVTRLEHREERFRLLVQNSTDVVTITAPNGRIHYISPAVRRVLGSEPEPMAGTNIAHRVHPDDREVVATNVAHVISRPGNSATYQARMSHADGSWRTLEIISVNLTDVPSVAGVVSNSRDVTETVQAHERLSYEATHDVLTGLANRALFGDRVQAGVANAGPGNTISIVLIDLDDFKTVNDTLGHSVGDGLLVAVADRMRASVRPTDIVARLGGDEFAILFEGVGGDTVDRVLERIADALAVPVEIDGHLLGVRASFGVVDGQAGDDDAGDLLRRADIAMYEAKERGEGGYQRYRPGMEARGAERNRLTAALLAALDQDELVLRYQPVVTLPEGRLTGVEALVRWQHPERGLLGPGEFIPGAEETGLIVPLGRWVLREACRQATAWIDDLGDLAPGTMSVNASARQLQEPGFAAEVAAALRDSGLAAGRLTIEITESTAVGGGATQDNLRGLRALGVRLSLDDFGTGASTLSLLANCPVDQIKLDRSFAPGPGPDAIAGAVLQLARALGVEAVAEGVETPAQAAKLGDLGYVRAQGFHFARPMTPEDVGAGIRARQVTELAGSFSARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPN3</accession>
    <name>U5VPN3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">zf-HC2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357715.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02415"/>
    <dbReference type="EnsemblBacteria" id="AGZ38772">
        <property type="protein sequence ID" value="AGZ38772"/>
        <property type="gene ID" value="AFR_02415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.488"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056526_1_0_11"/>
    <dbReference type="OrthoDB" id="1740922at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041916">
        <property type="entry name" value="Anti_sigma_zinc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027383">
        <property type="entry name" value="Znf_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13490">
        <property type="entry name" value="zf-HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="zf-HC2" evidence="2">
        <location>
            <begin position="7"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="24381" checksum="C0A2F9FCFC27B544" modified="2014-01-22" version="1">MSADHDDLRRLLGGYLLGGLDDTDTDRLDAHLLHCDECRAELDRLAPVPELLRSLENAPPVAVAAGTRPSPARIESLLGRMRAERTRDRRRTLGRRLAAVAAAVLLVAGAVGFIVSRDQDETPVPPPIAAAPSQPLITAVFEPSDGSGLAGQAVLTGKTWGVSIDLQVSKLSGAGPFVCRVSNADGQVEQAAIWGPTPSGNAKVTGASSIQLRNVSAVAVADTEGHVLGTAHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQT5</accession>
    <name>U5VQT5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05195"/>
    <dbReference type="EnsemblBacteria" id="AGZ39328">
        <property type="protein sequence ID" value="AGZ39328"/>
        <property type="gene ID" value="AFR_05195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1051"/>
    <dbReference type="eggNOG" id="COG0510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_673745_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="406" mass="43659" checksum="4E9EDF7136F0B78F" modified="2014-01-22" version="1">MKTAPAELTAGDAALRTQYLTEVLELLYPAPCDTAGGPGEQIAEYLVVPDARRPRLLVPNTSRKVAAAAVRRYAEPQSRSARLKRDAVVAALRTGASGVLLRDRVRITGPAAESIDGHLRESLGRELSVSIHIGPARANRKPVLQLISPEGETFGFGKLGTGPLTRHLVQAETAALRALGTVGLSKLTVPTVLHDGQWRGLQVLIQSALPVWLPRAPLDPGRLNAAMLDVAGCYGFTSGTLVGSAYWAELRGRLAAVGDRPEGVALTSAAELLATHAGQHSLQYGAWHGDWAPWNMANLADTLLVWDWERFTKGVPVGFDAVHYELQRRIQSTGDAAEAVEATVRRSAELLAPFGVAADVRELTALLYLVDLAARYLTDRQAEAGARLGVLGTWLLPVLIRRVEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQZ4</accession>
    <name>U5VQZ4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thiamine-phosphate synthase</fullName>
            <shortName evidence="9">TP synthase</shortName>
            <shortName evidence="9">TPS</shortName>
            <ecNumber evidence="9">2.5.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Thiamine-phosphate pyrophosphorylase</fullName>
            <shortName evidence="9">TMP pyrophosphorylase</shortName>
            <shortName evidence="9">TMP-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">thiE</name>
        <name evidence="13" type="ORF">AFR_04690</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 9">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 10">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 9 10">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 9 10">
            <text>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 9 11">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the thiamine-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.3" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358234.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04690"/>
    <dbReference type="EnsemblBacteria" id="AGZ39227">
        <property type="protein sequence ID" value="AGZ39227"/>
        <property type="gene ID" value="AFR_04690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.947"/>
    <dbReference type="eggNOG" id="COG0352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018272_3_4_11"/>
    <dbReference type="OrthoDB" id="1558094at2"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00141"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00097">
        <property type="entry name" value="TMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9 10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="6 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TMP-TENI" evidence="12">
        <location>
            <begin position="8"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="HMP-PP binding" evidence="9">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="THZ-P binding" evidence="9">
        <location>
            <begin position="138"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ39227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="20864" checksum="958E892BEA0FEC82" modified="2014-01-22" version="1">MGPVFPHLHLITGGQDPLAAVAAAVAAASRLGATDRLAIQVRVEDHVTDRDAYELADAVLELCRPHGVLCLVNDRLHVALAVGADGGHVGAADLPVAAARRVLGSSAMLGATCRDADAARAARRAGASYLGVGPAFATTTKTGLPDPLGVDAIGVVAAAVPDTPIVAIGGVTAKNAAGLRAAGAAAVAVVGAIMNATDPLRATEELLEAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR80</accession>
    <name>U5VR80_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="8">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AFR_05140</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8 11">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358837.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05140"/>
    <dbReference type="EnsemblBacteria" id="AGZ39317">
        <property type="protein sequence ID" value="AGZ39317"/>
        <property type="gene ID" value="AFR_05140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1040"/>
    <dbReference type="eggNOG" id="COG0538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023296_1_1_11"/>
    <dbReference type="OrthoDB" id="1551125at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="PTHR11822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 12" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Iso_dh" evidence="13">
        <location>
            <begin position="9"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="75"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="309"/>
            <end position="314"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="9">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="9">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ39317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="404" mass="45118" checksum="1C6FEF9742E318E1" modified="2014-01-22" version="1">MAKIKVNNPVVELDGDEMTRIIWKQIREQLVLPYLDVNLEYYDLSIQYRDETDDQVTIDAANAIKRHGVGVKCATITPDEARVEEFGLKKMWRSPNGTIRNILGGVVFREPIIMSNVPRLVPGWTKPIIIGRHAHGDQYKATDFVTPGPGKLTVTFTPEDGSAPMEFHVADYPGGGVAMAMYNYDDSIRDFARASFRYGLARNYPVYLSTKNTILKAYDGRFKDLFAEIFETEFAEKFKEAGITYEHRLIDDMVAAAMKWEGGYVWACKNYDGDVQSDTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYRQWQKGEKTSTNPIASIFAWTGGLKHRGVLDGTPAVTEFAETVEKVCVETVEGGQMTKDLALLISRDAPWLTTDEFMNALDENLARKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR94</accession>
    <name>U5VR94_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_05215</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358863.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05215"/>
    <dbReference type="EnsemblBacteria" id="AGZ39332">
        <property type="protein sequence ID" value="AGZ39332"/>
        <property type="gene ID" value="AFR_05215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1055"/>
    <dbReference type="eggNOG" id="COG4124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038234_2_0_11"/>
    <dbReference type="OrthoDB" id="669354at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665987" description="GH26 domain-containing protein" evidence="5">
        <location>
            <begin position="32"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="6">
        <location>
            <begin position="117"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="47"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="440" mass="48276" checksum="17FDC472F8B1CAF4" modified="2014-01-22" version="1">MRRRPPRTRLLHLIAVTAIFALSVVATLSWAWTGGVDQLGLPYGPGNDPTVTVPGRPLPPLGGARPTAEPGVPALPGQPTAVPSASGVPGASGSAAPGAEPAKPGPGQTALGGPRPSRAGNGECRTGRKLVPSCGVLWGVAPGAFTDKRGKQALVDFEKKTERHQDVYHAYHKGNRQLFPTDAEIDIARDPGKERILFLNWKPSGASWAKIAKGDKVTDAFLDRLAKHINREFPEQFFFTVHHEAEDNVKEKAGSGYTAEDYSAMYRHVVKRLRAHGVDNLVTVLVHMAYVPHTSKSWFDDMYPGDSVVDWIGFDTYAYSDPGYGHGDFAELLNRQSSARPNWPGFYNWAKREHPGKPLMVAEWGVWSSKKNPGHKAEFYREVGKQIVKFPNIRAMVHFDTPHNQEGRDSRVDATPEALKAYRKLGKLPIFQVHVAPSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRA6</accession>
    <name>U5VRA6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Trypsin-like serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358915.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05475"/>
    <dbReference type="EnsemblBacteria" id="AGZ39384">
        <property type="protein sequence ID" value="AGZ39384"/>
        <property type="gene ID" value="AFR_05475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1110"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_2_2_11"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="210"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="31301" checksum="F1BD696BC0B367D7" modified="2014-01-22" version="1">MDTEHTGVEAGALDAYSRVVTGVAAHLLPSVAALSVRTARGAGAGSAVTFTDDGFLLTNAHVVAGATGGTADFADGTETRFDVVGADALSDLAVVRVHNPGAPSAPLGDADGLRIGQLVVAVGNPMGLAGSVTAGVVSGLGRSLPARDGRRVRIIDNVIQTDAALNPGNSGGALADSTGTVVGINTAVAGYGLGLAVPINKTTRHIIGELVATGRVRRAWLGVAGVPAPLPPPIAERLGQKLGLRVVEVVPGSPAGTAGIYLGDILITAGGEPVQTVQALQRLMLGPAIGTNLPITVLRKNAFVDVVTVPAELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSI8</accession>
    <name>U5VSI8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA polymerase III subunit delta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_01930</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357618.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01930"/>
    <dbReference type="EnsemblBacteria" id="AGZ38675">
        <property type="protein sequence ID" value="AGZ38675"/>
        <property type="gene ID" value="AFR_01930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.391"/>
    <dbReference type="eggNOG" id="COG0470">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006229_4_1_11"/>
    <dbReference type="OrthoDB" id="1971388at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004622">
        <property type="entry name" value="DNA_pol_HolB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00678">
        <property type="entry name" value="holB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="39"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="41611" checksum="A3E375B41906765F" modified="2014-01-22" version="1">MSVFADLVGQDEAVETMSRAAAAAARIVAGDNVTANGAMTHAWIFTGPPGSGRSVAARALAAALECERDGTGCGECHGCHTVLGGTHADVRFVVPEGLSIGVNEMRALVLRAGTAPSGGRWQAVVVEDADRLTEAAGNALLKAIEEPPPRTVFLLCTPSTHPDDISVTIRSRCRVVPLRQPPAEAVAEMLVRRDGIAPDVASWAAAAAQGHVGRARRLANDPEARGRREAVLAVPRRLTGVGACFDAASALIAATETEAATAFAENDSTERAALEQALGAGGTGRGAAGAMRGSAGQIKELEKRQKSRSTRSQRDALDRALVDLAGFYRDVLVTNLRAPVAVVHSDIAQQSSAAAEKWSPESTLRRLEAILACRDAIEQNVKPKIAVEAMMLNLWRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSR6</accession>
    <name>U5VSR6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357619.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01935"/>
    <dbReference type="EnsemblBacteria" id="AGZ38676">
        <property type="protein sequence ID" value="AGZ38676"/>
        <property type="gene ID" value="AFR_01935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.392"/>
    <dbReference type="eggNOG" id="ENOG5031MNN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2191308_0_0_11"/>
    <dbReference type="OrthoDB" id="1866113at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="108" mass="12108" checksum="5877CD805EA8C7DD" modified="2014-01-22" version="1">MPREIDEAWIDEAIDRYRRLDVLRGEFEKAVAGHVVSVHSPDDSIEVLVTAAGEITDVRIRGSLQHRSAAELAREVQAVVTTATEAARWARQKLHDEVFGAFRSLGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT08</accession>
    <name>U5VT08_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative GTP cyclohydrolase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08995</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359624.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08995"/>
    <dbReference type="EnsemblBacteria" id="AGZ40088">
        <property type="protein sequence ID" value="AGZ40088"/>
        <property type="gene ID" value="AFR_08995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1830"/>
    <dbReference type="eggNOG" id="COG0807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_2_1_11"/>
    <dbReference type="OrthoDB" id="900513at2"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="GTP_cyclohydro2" evidence="6">
        <location>
            <begin position="23"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="21631" checksum="89EF160220C76084" modified="2014-01-22" version="1">MSLGIRSRVTVPLKFADGYSTTAEVITFDGLADGKEHLALALGDAFGAEVPLVRPHSECLTGDVFGSERCDCGPQLREAVERIAGVGGFLLYLRQEGRGIGLYAKLDAYALQDLGLDTYEANRALGHADDERDYTAAAQMLTALGAGTVDLLTNNPDKPAQLRASGVDVRAVVPTQVHASDANVRYLQAKVSHTAHTIALPLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT60</accession>
    <name>U5VT60_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357858.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38915">
        <property type="protein sequence ID" value="AGZ38915"/>
        <property type="gene ID" value="AFR_03130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.630"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_1_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="30743" checksum="8BA7E6922B6249E1" modified="2014-01-22" version="1">MDRAQLADFLRTRREALQPEDVGLPRGARRRTGGLRREEVAALCGMSADYYSRIEQQRGPHPSEQMLAAIARGLHLSLDERDHLFRIAGHATPQRVHRADHLNPGMMRILDRLEDTPAQVMSGLGETLRQTRSCIALLGDDSRWTGMARSMVYRWYTEPESRLIYPEEDHPEHGRVFTAQLRIAYARDGLNSRAAAVVDALRAVSPEFIAVWAEHDVTSLHTDQKRISHPQLGLLELHCQTLVDPDQSQALLVFTAVPGTESYEKLQLLSAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTE6</accession>
    <name>U5VTE6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357879.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38936">
        <property type="protein sequence ID" value="AGZ38936"/>
        <property type="gene ID" value="AFR_03235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.651"/>
    <dbReference type="eggNOG" id="COG4922">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073327_1_0_11"/>
    <dbReference type="OrthoDB" id="1598464at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436">
        <property type="entry name" value="PTHR38436"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="140"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="252" mass="27093" checksum="BA07383A480A2AC5" modified="2014-01-22" version="1">MTAKDVVLSAAGQLFGDKDPSAVDRWVGPAYVQHSSLAADGPEGLRQLVQNLPAGFRYDLHRVIADGDLVALHGTYHGFGPDPLVAFDIFRVADGKLAEHWDALTPQVDKTVSGRSQTDGATEVTDLDATDANRDLVVGFVETVLKGGKVDTITDYLSAEKYWQHNTGIGDNLDGLGAALGALAEQGVSMVYDTVHKVVAEGDFVLTVSEGRFGVTPTAFYDLFRVENGKIVEHWDITPEIKGDLPHSNGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTL5</accession>
    <name>U5VTL5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359853.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09575"/>
    <dbReference type="EnsemblBacteria" id="AGZ40204">
        <property type="protein sequence ID" value="AGZ40204"/>
        <property type="gene ID" value="AFR_09575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1952"/>
    <dbReference type="eggNOG" id="COG0380">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_7_1_11"/>
    <dbReference type="OrthoDB" id="556566at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="PTHR10788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="470" mass="52291" checksum="15E9D921195010AE" modified="2014-01-22" version="1">MRQSSLVVVANRLPLDDNAAPDGACEWRRSPGGLASALHAILQQTPATWIGWNGGIDSAPVLPDVGTLRIRPVSLSSEELQGYYEGFANSTLWPLYHDAVEQPVFDRGWWETYRAVNQRFAEAAAELAEPGAAIWIQDYHLQLVPQLLRELRPDVLIGFFLHVPFPPPELFMQLPRRAELLRGMLGADLVGFQRPQAAHNVAQLAVKLFGAEATDDQITLDGRTVRTGAFPVSIDVTEMQALASRPDIVEHAEQLRADLGQPRRVLLSVDRLDYTKGIEHRLTAYSELLRDGRVKVRDTVMVQVAVPSRERVDSYRVLRDRIEGEVGRINGEYGRVGEPAIHYLNQSFDRADLAALYQTADLMVVTPLRDGMNLVAKEYVAARSDNSGALVLSEFAGAAAEFEDAFLVNPHDVDGLKETLLQAMDADPADLQRRMAAMRDQLQRHDILAWARTYLTALDHTGRLAAGLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTT8</accession>
    <name>U5VTT8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protoheme IX farnesyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Heme B farnesyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ctaB</name>
        <name evidence="9" type="ORF">AFR_10645</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate + Fe(II)-heme o</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10645"/>
    <dbReference type="EnsemblBacteria" id="AGZ40418">
        <property type="protein sequence ID" value="AGZ40418"/>
        <property type="gene ID" value="AFR_10645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2170"/>
    <dbReference type="eggNOG" id="COG0109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029631_0_1_11"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PTHR43448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ40418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="321" mass="35172" checksum="EA5859EC5281B0E0" modified="2014-01-22" version="1">MSMITERPDQGRSPDLLPAAPREAANAKSRRRDLGAVIKAYVSLTKPRIVELLLVTTVPAMMLAAGGWPDLWLVAVVLVGGSLAAGAASALNCYIDRDIDQLMRRTKRRPLPAHVVTPRSALIFGLVLAVISVVLMAVFTNWLATAMTAGSIFYYDVVYTLWLKRRTPANTFWGGICGAAPVLIGWAAVTGSIAPLGWALFAIVFFWQMPHFYALAIKYKDDYARAGIPMLPVVRSARRVNTEILLFTVLMLAASFAAWPLGLGPIYGVTAIVTGLVFLLEAVKLVNRSRRGEPLKPMRLFHWSYTYLTILFIAVAVDALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU55</accession>
    <name>U5VU55_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Triosephosphate isomerase</fullName>
            <shortName evidence="3">TIM</shortName>
            <shortName evidence="3">TPI</shortName>
            <ecNumber evidence="3 4">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">tpiA</name>
        <name evidence="5" type="ORF">AFR_10575</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="3 4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360247.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10575"/>
    <dbReference type="EnsemblBacteria" id="AGZ40404">
        <property type="protein sequence ID" value="AGZ40404"/>
        <property type="gene ID" value="AFR_10575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2156"/>
    <dbReference type="eggNOG" id="COG0149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024251_2_3_11"/>
    <dbReference type="OrthoDB" id="1266295at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3 4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="3 4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="242"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="3">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28030" checksum="0650C2DF7A7FB237" modified="2014-01-22" version="1">MADVSRRPIMAGNWKMNLNHFEAILLVQKLAASLNEQQLTDVEAVVLPPFTDLRSVQTAIDGDKVLIGYGAQDLSPHKGGAYTGDISGAMLAKLGCSYVVIGHSERREYHNENDALVNAKVKAALANDLTPILCVGEGLDVREANGHVEHCASQLDGALDGLKAEQVKNIVIAYEPVWAIGTGKTATPENAQEVCGSIRVRLAEKYGAETADAVRIQYGGSVKAANIASIMAQPDVDGALIGGAALDAEEFASIVRFREHVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUT0</accession>
    <name>U5VUT0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05610"/>
    <dbReference type="EnsemblBacteria" id="AGZ39411">
        <property type="protein sequence ID" value="AGZ39411"/>
        <property type="gene ID" value="AFR_05610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1138"/>
    <dbReference type="eggNOG" id="ENOG5030HT7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_433228_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="631" mass="65799" checksum="6318A0A987CE35FC" modified="2014-01-22" version="1">MTDTLFLVVAADGYGPSVRHLRSIIYALVLAPAVWILCGVGFDQDLTGRARDNGGIESLSGVLLLLLAGAAYSILLFSPISPLGPLLAGLAFVGVGAWARLAPDQYAGIWPPNVVKEGFDVSTPGYGLAVLLAVPMLCTALSARRWKGFEPPEILLIGTIGRARGAAPVAGTPIAAERTTVIPQQRQYQVPPAQSFPPPQSFPQGQPTYGTPAATPFPANGDENTTVVPAQATTVLPQEDDEEKTTVMSLGRPAASAPTSPARPPADEVTTLLTPPGRPHEEPATIAVSSPADDRTEVLTSAHEGSVAGAAAPDEFSNQTTREVADSESTRDVVSDQPTHDVAASENTADVSADEATRDMSADEATRDMSADEVTRDVAGDEATRDVGGDEVTRDVVAEDDEPTRPVIADEARGEETTRDAGAGQVTHDLAGDETTRLAVVPPAPGRYEPHGSGEETTRLVFPAAAEAQGDNDKTEALQLPAATDGERTQLIRSGTVQPPGDRTQLLTFPTPGEAATARNGAADPPGETASRPDEAVTRAATGPGEAATVVNPVGEKTHESTKAMSIVGEERPDPGEDPTTRLSAVRDPGQVTEPRRGAMSATNLERPADEAQDDTRPLTLPAPREATKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUU1</accession>
    <name>U5VUU1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP_transf_9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359036.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06080"/>
    <dbReference type="EnsemblBacteria" id="AGZ39505">
        <property type="protein sequence ID" value="AGZ39505"/>
        <property type="gene ID" value="AFR_06080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1235"/>
    <dbReference type="eggNOG" id="COG2343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126578_1_1_11"/>
    <dbReference type="OrthoDB" id="2063459at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007361">
        <property type="entry name" value="DUF427"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038694">
        <property type="entry name" value="DUF427_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04248">
        <property type="entry name" value="NTP_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_9" evidence="1">
        <location>
            <begin position="3"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="10720" checksum="6827B658F99283C6" modified="2014-01-22" version="1">MPKAIWNDEIIAESDDTVVVEGNTYFPRSSVREELLRPSDTHSICPWKGKASYYSIESGGQVNEDAIWYYPEPKPDAKAVRDRVAFWKGVKVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV08</accession>
    <name>U5VV08_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360658.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11690"/>
    <dbReference type="EnsemblBacteria" id="AGZ40627">
        <property type="protein sequence ID" value="AGZ40627"/>
        <property type="gene ID" value="AFR_11690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2381"/>
    <dbReference type="eggNOG" id="ENOG5033Q6Y">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_670327_0_0_11"/>
    <dbReference type="OrthoDB" id="1184828at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666106" evidence="1">
        <location>
            <begin position="27"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="387" mass="43208" checksum="4F0FF8C6ECB18E6E" modified="2014-01-22" version="1">MTAKDAKIGRRSVLLLALASLTAASASPSTDVLPMMNPLPAAPTAAAGTIDLKPVSHWTAVFMDSWDYQYENSLPMSRSKDSWDHYDLSYSVDSCVAMFRATDQRRYLDRALEFVENVVASAVVSSTLRSSGYKDKYRGWASSASGQGGDEVPLYESYFWRYGTALLVAMHDAPAVLADAAYRQRYERLLRFAEVDIFEKWYSRSPGENIYRERTHMAAHWALIAMNLAQVTTDDGRRTRYQQVVDNVDLHVPGVGSSLRAQMRRNPAEPTAYFWSDVWGSARRPGQDVSHGNGVMAYVVEARDRGRQWTAADLAGFSALLTRVIWPGGTTYRNFVDGTGSDNGWFSDGFVKLGRSDPAVQRRLEDHQVVNDQFAANMALNARVLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV17</accession>
    <name>U5VV17_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11740"/>
    <dbReference type="EnsemblBacteria" id="AGZ40637">
        <property type="protein sequence ID" value="AGZ40637"/>
        <property type="gene ID" value="AFR_11740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2391"/>
    <dbReference type="HOGENOM" id="CLU_136716_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021660">
        <property type="entry name" value="DUF3253"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11625">
        <property type="entry name" value="DUF3253"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="132" mass="14318" checksum="5CE7CD3C4E5EB2AA" modified="2014-01-22" version="1">MEEAIPEALRRELMSERETAATPARLQDARVALGEDSKAGDRARLAATVRALLWHRRPESTICPSDAARVVGGESWRELMDVAREVAAGLARDGTIVVRQRGEDVDITTAAGPVRLARGPKWDPAGRIDPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVP3</accession>
    <name>U5VVP3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, TetR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_13290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361003.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13290"/>
    <dbReference type="EnsemblBacteria" id="AGZ40944">
        <property type="protein sequence ID" value="AGZ40944"/>
        <property type="gene ID" value="AFR_13290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2698"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082427_0_0_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="232" mass="24773" checksum="553C919EFD5DCB2B" modified="2014-01-22" version="1">MDDESSRRQQILEVAAEILERDGIQAVSTRTVAAAAGIRAASLYQLFGDKDGLLDALAIQAFERYLAGKDSLERSADPLDDLRRGWDMHVEFGLRHPALYVLMFGTDRPGRQPPAAAEAHDRLLSFLVRVADAGRLTTPPAVAAQLVQAAATGITLSLIASPAGQRDPQVATKMRELMVASLTTDSPPPSDPGLATRALALDAALSGADDKALPLRASEVALLRDWLNDLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVZ9</accession>
    <name>U5VVZ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359431.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08055"/>
    <dbReference type="EnsemblBacteria" id="AGZ39900">
        <property type="protein sequence ID" value="AGZ39900"/>
        <property type="gene ID" value="AFR_08055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1644"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_3_11"/>
    <dbReference type="OrthoDB" id="1439833at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="21"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="29372" checksum="676898A34AF5EC55" modified="2014-01-22" version="1">MQIQARGLTFDVHEGGPADGPPVLLLHGFPQDHREFDLILPRLHAAGLRTYALDQRGYSRGARPAAVADYRLDEPTADAIAVLDALGVEKAHVVGHDWGAQVGWLLADKHPGRVSTLTAISVPHPRGLGVALRNQPSQKLRFAYMGVFRSRVAERLLLAGNETVLRQMLRPLGARAGQYAVAMREPGRLTGALNWYRALTGHQLGTVGKITVPTTYVWSDGDPVVGRPAALATATWVTADYRFVTLRGIGHWVPEEAPQALADVTLARIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW39</accession>
    <name>U5VW39_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13790"/>
    <dbReference type="EnsemblBacteria" id="AGZ41044">
        <property type="protein sequence ID" value="AGZ41044"/>
        <property type="gene ID" value="AFR_13790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2800"/>
    <dbReference type="HOGENOM" id="CLU_1821234_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665804" evidence="2">
        <location>
            <begin position="38"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="141" mass="13727" checksum="B8DC883606D6E9AB" modified="2014-01-22" version="1">MRVHSFQHRRYGRVIAAVGLTVLLGFTGACGGSDDKAAGTTATADATAAPADEPAGDCGAAQAEIKASLTGMGAVTSVDVPTCDKAVVATTLGADEGDQAASLCQNAANEASTHGVAAVSVVSADGKELATGTTAEDCKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW63</accession>
    <name>U5VW63_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359432.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08060"/>
    <dbReference type="EnsemblBacteria" id="AGZ39901">
        <property type="protein sequence ID" value="AGZ39901"/>
        <property type="gene ID" value="AFR_08060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1645"/>
    <dbReference type="eggNOG" id="ENOG5032T9G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087620_2_0_11"/>
    <dbReference type="OrthoDB" id="2003359at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046291">
        <property type="entry name" value="DUF6328"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19853">
        <property type="entry name" value="DUF6328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="175" mass="19839" checksum="5F55E75768161BF0" modified="2014-01-22" version="1">MKHETEKQRWDRNFADLLQELRVAQTGVQILFAFLLTLPFSSGFPDTTQFQKDTYVVALIAAAFATAMIISPVAFHRALFRQGRKPELVRYAHRMATGGLAFMLIAMVSSVLLITDYLVNIWVAIILTAVTAGWFLTFWAAIPFVRRNWMDEDEEEQDEEYEAEAAEPASAASRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWK8</accession>
    <name>U5VWK8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361231.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14450"/>
    <dbReference type="EnsemblBacteria" id="AGZ41172">
        <property type="protein sequence ID" value="AGZ41172"/>
        <property type="gene ID" value="AFR_14450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2933"/>
    <dbReference type="eggNOG" id="COG2355">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012378_0_0_11"/>
    <dbReference type="OrthoDB" id="167885at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666150" evidence="1">
        <location>
            <begin position="28"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="672" mass="70754" checksum="D6BCDC20B8758EEC" modified="2014-01-22" version="1">MPLSSLRRPVALALALLLPATALQAPAAAAAAGITVAADALDGVCAAIQAYDGTRTIGYVVRSSTGYGFTNSAASATPFRLEATQLSRYELFDNTGAPAYQSVAGFILPGDAYGDRADWTVSPSGDRYRFTSTATGQVMGQFLWGLGSGSTSSSMALTPATGCWTPPDIDPAVSGTAAPGMVGTIDAHAHITAGAAFGGRLHCGEAWAPGGVRDALAGCASHASLNVGALFEGIIGGTDVINSAEDGWPTFGDWPQHNSLLHEQSYYRSIERSWRSGQRVLNALLVANRVICEIFPYRDTSCDEMDQLRVQATYLYSMQDYIDAQHGGRGKGWFRIATTPAQVRTIAAQGKLAVLVGVENSEVFGCREINDVAQCSRADIDAGLDELQALGVSGLYPVHKFDNAFGGTRFDSGVTGAALNVANLLSTGHWWTATSCTGPSDNEQPLVSDDFAELLKLGPLDLPAGAVLPVYPSGPICNTRGLTALGTYLIQQMISRGMIIHVDHMGVKTAQAVLDLADAADYPGVTSVHTWADPALVERILARGGFVATYAFAASEFLTEWRANRALPHGASITGYGFGSDVNGLGPQAPPRGTDPLTYPFTAPNGTTVSKQVYGSRTFDLNTDGVAQYGLYADWTTDLIRQAGPDGPALRQQLMNGAEAYTRMWEGAIAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWL3</accession>
    <name>U5VWL3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peptide chain release factor subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_14475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361236.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14475"/>
    <dbReference type="EnsemblBacteria" id="AGZ41177">
        <property type="protein sequence ID" value="AGZ41177"/>
        <property type="gene ID" value="AFR_14475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2938"/>
    <dbReference type="eggNOG" id="COG1503">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054531_1_0_11"/>
    <dbReference type="OrthoDB" id="1375098at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040701">
        <property type="entry name" value="Bact_RF_family2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18844">
        <property type="entry name" value="baeRF_family2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="374" mass="40142" checksum="DDFF547D6DA89939" modified="2014-01-22" version="1">MNLDFLRPLFDQPGPWVSVYLDATRASENADHEVNLRWRGLRELLEQQGAEAITLDAVQRAVDEHPYQPGRYGLAIFARAGEAVLVETLSAPPPADEAHLGSLPHAMPLVAQRGEEVPYVRVLADRTGADLDALSVGGAPRHRTVTGSAKFPLRKVQVGGWSHRHYQQAVEESWKHNADDVAAAAVQLAETVGAEVIVAGGDVRAVQTLAGRLPKRWQERLVQTDAGARHAGADEEALDDVTIQAVADIADRTTREAIDRYQAQRGSGQASTGLTDVVTYLQRGQIDTVLLVNDLSSTDTLWIDPGDPTLVSVDDHVLREAGVQNPQKVRADAALLRAIAGTGAHLVLLGPDEVPLDHGIGGVLRYADASSAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX11</accession>
    <name>U5VX11_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Family 2 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359954.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09830"/>
    <dbReference type="EnsemblBacteria" id="AGZ40255">
        <property type="protein sequence ID" value="AGZ40255"/>
        <property type="gene ID" value="AFR_09830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2004"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038143_0_0_11"/>
    <dbReference type="OrthoDB" id="724641at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="38"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="38491" checksum="D2A9CC7A63FDC97E" modified="2014-01-22" version="1">MIWPALLPWVALTGHTIVNAALLRRLSAGQTSAERIAVLLPLRNEAERVTPCLESLLNQRGVRNLEIIVLDDGSTDGTADVVRAAAPGSTVRLLTGAALPPGWLGKPHACAQLADAAADADVLVFLDADVVLAPDAIAGAATLLRKTGVDLLSPYPRIVGAGRLVQPLLQWSWLTFLPLRIMERSPRPSLAAAGGQWLVVDRAGYERAGGHAAVRDDVLEDIGLARAVKRSGGRIALADASGFADCLMYTSWPDLRDGYSKSLWASFGSPAGAAAVVTLLILLYAVPPLAALVLAASHPVLALWSLAAYGIGVVGRVVSARATGGRAWPDALAHPISVLIFGWLVVRSFRLRRRGLLAWRGRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX13</accession>
    <name>U5VX13_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09460"/>
    <dbReference type="EnsemblBacteria" id="AGZ40181">
        <property type="protein sequence ID" value="AGZ40181"/>
        <property type="gene ID" value="AFR_09460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1929"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1501861_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="26"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17169" checksum="654747790A972EE5" modified="2014-01-22" version="1">MRGVAYQTFYRLPHPVRRRVVRLIVPKYLVGAVAIIKDSEAADPGRLLLLRQPPGRSWGLPAGLLKKAEAPEVGAARELAEESGVVLDPTDLTPATPNAVVHAKGWVDMIYLGQVPASTTPLVVDGGEVLEAGWFSLADLPRLTPNTAKVLGLYGMGPNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXA2</accession>
    <name>U5VXA2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361481.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15700"/>
    <dbReference type="EnsemblBacteria" id="AGZ41422">
        <property type="protein sequence ID" value="AGZ41422"/>
        <property type="gene ID" value="AFR_15700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3188"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="275" mass="28144" checksum="EAAFE5E269A398D9" modified="2014-01-22" version="1">MRGLTGKVAVVTGAAGGIGAATARRLAAEGAHVVAVDTDEDRVRQVVESLETEGTWVRADVSAEDGVAGYVTAAVDRFGRIDLHHLNAGIPGSFATLPELTVDEFDRVLAVNVRGVFLGVRAAFRQYAAQGGPGNVVITGSIGSLRGSADLLAYQTSKHAVLGILRGAAMYGGPLGIRVNAVAPGIVPTDLFAATPDVSGGKDDMSRRASSTPLRRAGTGDDVAGVVAFLLSDDAAYMTGEVVSVDGGASIVSTVRPSGGAGAWDFEAYDRALYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY65</accession>
    <name>U5VY65_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyridine nucleotide-disulfide oxidoreductase dimerization subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_17780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="2">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17780"/>
    <dbReference type="EnsemblBacteria" id="AGZ41834">
        <property type="protein sequence ID" value="AGZ41834"/>
        <property type="gene ID" value="AFR_17780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3606"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_1_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="5">
        <location>
            <begin position="2"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="4">
        <location>
            <begin position="347"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="136"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="173"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="3">
        <location>
            <begin position="34"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="462" mass="48002" checksum="982177954EC5080A" modified="2014-01-22" version="1">MLGAGPVGENVADRAVRGGLTVVLVEKELVGGECTYWACMPSKALLRPAQAVRAARQVAGAAQAVTGGLDVQAVLDRRDSFTSNWKDDGQVAWVEGAGISLIRGQGRLTGVREVTVTAADGTTTVLTARHAVAVATGSDALVPDIEGLRDADPWTSREATSAKEVPASLAIIGGGVVATEMATAYLSLGSTVTLISRSGLLAGMEPFAGEAVAEALRKQGATVLLDTETTRVTRTGEEVAIETSGGTTVTAAEVLVATGRTPRTRDIGLDVVGLKPGDWISTDDTMRVEGFDWLYAAGDVTHRALLTHQGKYQARAAGDVIAARAQGTPVSDAPWGKHVATADHSAVPQVTFTDPEVASVGLTAAAAQKAGIRVRVVDYEIGQVAGASVHADGYQGKARMVVDEDRQVVAGVTFVGPDVSELLQAATIAVAGEVPIARLWHAVPAYPTISEIWLRLLETYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYX9</accession>
    <name>U5VYX9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360940.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12975"/>
    <dbReference type="EnsemblBacteria" id="AGZ40881">
        <property type="protein sequence ID" value="AGZ40881"/>
        <property type="gene ID" value="AFR_12975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2635"/>
    <dbReference type="eggNOG" id="ENOG502ZBW7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093484_0_0_11"/>
    <dbReference type="OrthoDB" id="1918376at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="19134" checksum="CC510D23752A7970" modified="2014-01-22" version="1">MSTVAASPGISQTAAALRRLYFVRAAFAIVWAVVTIATAKEIGPLTVTLFVLYPLFDVGAAVYDLRSSRSSGSQTLLYVNIVVSLITAIGLGAAAASGIPAMLRVWGAWAIVAGLVQLFVGVSRRRMGGQWPMIISGAISVLAGGSFVAGASADNPMLTNAAGYAIPGAIFFLIAAFRLGRAAKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ92</accession>
    <name>U5VZ92_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PAS/PAC sensor-containing diguanylate cyclase/phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_20180</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362680.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20180"/>
    <dbReference type="EnsemblBacteria" id="AGZ42308">
        <property type="protein sequence ID" value="AGZ42308"/>
        <property type="gene ID" value="AFR_20180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4093"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044810_0_0_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="181"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="256"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="339"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="480" mass="51887" checksum="4A2D9E724CDA3A17" modified="2014-01-22" version="1">MHPLLARGARIARNGMEPLVVLTLVVVLRQFDLTGDVPLWILALVLGFGAFCQQPEIQLWLSGGNLHRRAGLRISLHIVNTTITMYLLGWGPLLAIAHLHILTVHLRQSGSRAWKPAAAASAGALLAGQVVVGLGLLPTNLSVAQSHGLALLVAIGTVTTARTLGKFVAQNERAEASVRHNEERFRALVRDGSEVITMGDADGNITWVSPAAKPVMGYLPEELRGKVLSGLFHPEDDIAAMELFTRLLASDSTVEHSAELRVRHADGSWHWHEIIARNMLAHPAVQAIVSHQRDITERRAVQDRIAYAASHDGLTGLANGPTLNRDLERALAQGTRYQHPVAVLFCDLDGFKAVNDTYGHDVGDRLLQTISGVIKRVTRDTDTAGRLGGDEFGVVLTRVRNAEEALSVAQRIVEGISTNASVAGLKLDVGCSIGVALAYPGGSDVKSLMRHADAAMYRSKRQGRNGCQLYVEEEVTAPWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZG1</accession>
    <name>U5VZG1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362529.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42157">
        <property type="protein sequence ID" value="AGZ42157"/>
        <property type="gene ID" value="AFR_19425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19425"/>
    <dbReference type="HOGENOM" id="CLU_1912568_0_0_11"/>
    <dbReference type="OrthoDB" id="1959257at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="132" mass="14166" checksum="AE2F459C00F3C745" modified="2014-01-22" version="1">MNGELAVVEEWLTAINSGDGARVESLSHEEVEIVGPRGRGRAPRAILSEWLGRSGFSSDPVRWFCGGDGRVVVEQQARWTDPATGAAQDSFRIGSFFRVTGGRVAAYERFDAGVAAALTAAGLDPERDEATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZG6</accession>
    <name>U5VZG6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc metalloprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_20605</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20605"/>
    <dbReference type="EnsemblBacteria" id="AGZ42393">
        <property type="protein sequence ID" value="AGZ42393"/>
        <property type="gene ID" value="AFR_20605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4178"/>
    <dbReference type="eggNOG" id="COG3227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4935">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008590_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011096">
        <property type="entry name" value="FTP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023612">
        <property type="entry name" value="Peptidase_M4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001570">
        <property type="entry name" value="Peptidase_M4_C_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013856">
        <property type="entry name" value="Peptidase_M4_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07504">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01447">
        <property type="entry name" value="Peptidase_M4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02868">
        <property type="entry name" value="Peptidase_M4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00730">
        <property type="entry name" value="THERMOLYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665902" evidence="8">
        <location>
            <begin position="34"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="9">
        <location>
            <begin position="620"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623612-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ42393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="742" mass="75163" checksum="95BFB0FE4BC610CA" modified="2014-01-22" version="1">MGRKRLITASVALATGAALTLAAAPATAGPQQAPSPAPAPAALAASSADRLVDSAASAGVLRRAAGDDLKRTGVVQGTRGLQYVTYARSFKGLQVVGGDVVVTTDAAGAVKDTAVAQTAEISVGTTAKVTAAQATATAKSRLAKVDRAEAPTLKVLAWGSPKLVYEAVVEGRTAQNKPSNLHIFVDAVTGGVVDQVDYVREGTGNGYYYGNVTINTSGSGSSYSMTDTTRPGLRCGGQGGTAYTGTDDAWGTGSGTNLETACVDALWGAQREIDMFSSWFNRNGILGNGNNPPIRVGLNDVNAYWNGSYVNFGHNQANNRQATPIDVVGHELGHAVFQTTPGGAGSGNENGGINESTGDIFGALTEAYANSPLDPPDYEVGEEVDLVGQGPIRYMYNPSLAGDPNCWSTSIPSTEVHAAAGPLNHWFYLTAEGSAPSGKPASPTCNSTSVTGLGIRKAGEIYYNAMQSKTSTWKYANIRTATLAAAKNLYTPSCAEFNIVKAAWAAISVPAQSGEATCTGGTPTNDFSVSASPTSGSVAAGSSATTTIATATTSGSAQTVALTASGLPSGATASFSPASVTSGSSSTLTIATTASTPSGSYTVTVTGTGSATRTATYTLTVTGGAGGSCTGTNGTDVSIPDAGSAVTSTITISGCGRNASSTSTVAVNIVHSYRGDLVVDLVAPDGTSYRLKNSSSSDSADNVNTTYTVNVSSEAGNGAWKLQARDIYSVDTGYINTWTLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZM9</accession>
    <name>U5VZM9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Xanthine/uracil permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_19775</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362599.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19775"/>
    <dbReference type="EnsemblBacteria" id="AGZ42227">
        <property type="protein sequence ID" value="AGZ42227"/>
        <property type="gene ID" value="AFR_19775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4013"/>
    <dbReference type="eggNOG" id="COG2233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017959_8_2_11"/>
    <dbReference type="OrthoDB" id="1419334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017588">
        <property type="entry name" value="UacT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03173">
        <property type="entry name" value="pbuX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01116">
        <property type="entry name" value="XANTH_URACIL_PERMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="459" mass="47896" checksum="A3E2A75DCE9FFA04" modified="2014-01-22" version="1">MSQPREVAVPVEKHPVDQMLPFGRLAVLGLQHVLVMYAGCVAVPLIVGGALGLSTDVIAILVNADLFVAGVITIVQSFGVGKILGVRLPIVAGATFTALTPMILIGSKYGLPAVYGSMLAAGVFGLLIAKPFSKIIHLFPPLVTGTVITVIGLSLIDAGAGLIAGNDESDPGYGNLSHLALAAAIILLIVLINRFARGFLVSLAVLIGLVFGVIVASFMGLVDFSSVGDAGWFGLARPFYFGAPEFPVAAIISMCIVMLVIYVESTADMLAVAQLVDKKLTENDIARGLAADGVSGILAGTFNSFLDTAFAQNVGLVQVTRVRSRYVVTAAGVILVLLGLIPKLGEIIAAVPGPVIGGAALVMFATVTAVGIRSLRRVRFDGNNNLLIIAVAIGAGMVPVVAPSFYAKMPDEFQIIFGSSITTTVLVVFLLNLLFNHLKWRRESSEPSDEEVPSDSPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZS4</accession>
    <name>U5VZS4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monooxygenase FAD-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361274.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14665"/>
    <dbReference type="EnsemblBacteria" id="AGZ41215">
        <property type="protein sequence ID" value="AGZ41215"/>
        <property type="gene ID" value="AFR_14665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2978"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="4"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="472" mass="51717" checksum="020546F90D610A85" modified="2014-01-22" version="1">MNHDVIIAGCGPAGAMLAAELRLHEVRVLVLEKDTEPVSFARIVGLHIRSLELLAMRGLLERIAAHGRRRPAGAYFAAIDRPAPAEVDSAHAYLLGIPQPVLVRLLEEHTIDQGTQVRRGCAVTGLEQDDNGVTVELADGERLRARHLVGCDGGRSTVRKLLGVGFPGEPSRTETLMGEMAADLPQPELAASDRRLSLRPAGAGLYRVVVPAAGVSDRADPPTLEDFRQQLRAVAGTDFGVHSPRWMSRFGDATRLADRYRVGRVLLAGDAAHIHPPLGGQGLNLGVQDAVNLGWKLAAEIRGWAPETLLDTYQSERRPVAADVLDNTRAQTELSSDEPGPRALRRLFLELMEFDEVNRHLLEKITAIGVRYDFGPGPDLLGRRLRDLDLGQGRLYDLMHRGRGLLLDRTGRLNADGHRDRVDRVGDPTADLEVPAVLLRPDGHVAWIGDDQRDLDDHLTRWFGGATSIDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZV0</accession>
    <name>U5VZV0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA polymerase factor sigma-70</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_14470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361235.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14470"/>
    <dbReference type="EnsemblBacteria" id="AGZ41176">
        <property type="protein sequence ID" value="AGZ41176"/>
        <property type="gene ID" value="AFR_14470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2937"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043648_1_0_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014305">
        <property type="entry name" value="RNA_pol_sigma-G_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02960">
        <property type="entry name" value="SigX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="16"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="126"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="320" mass="35893" checksum="357AB0EAA1D5EDED" modified="2014-01-22" version="1">MSTDTRPVDEPAFSGLAERHRRELHVHCYRMLGSFEDAEDTVQETFLRAWRRRETFEGRSTFRTWLYRIATNACLDRLARSRPEPATGGEVPWLQPYPDRLLDELPAGDADEPESLAVARETIELAYLVAVQHLAPRPRAVLILRDVLGWPARDVAELLGDSVNSVNSALQRARAGMREHLPTERQDWTGGQEDAGTRELVRRFTDASVATDIDRLAVLLRDDVRSSMPPTPGLSAGRDAVVAGWVEDGFAGMTGLRAVPTSVNRQPAVAFYHWREQERAYLPLTLDVLRITGGAITEIVTFGADRFARLELPERLLEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W024</accession>
    <name>U5W024_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361389.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15240"/>
    <dbReference type="EnsemblBacteria" id="AGZ41330">
        <property type="protein sequence ID" value="AGZ41330"/>
        <property type="gene ID" value="AFR_15240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3094"/>
    <dbReference type="eggNOG" id="COG1359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154488_2_0_11"/>
    <dbReference type="OrthoDB" id="1909149at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="5"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="101" mass="10618" checksum="9772F17AEF74E3C5" modified="2014-01-22" version="1">MTIDHGFHATMTAQPGRGDELIRLLLAAPSLKNEDCVVFLVGRSASDSDVVHVTEGWTTPEAHSAFFATAEAQALVAQLQPLLAGESVYTDEVPVGGKAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W036</accession>
    <name>U5W036_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type 12 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362871.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21135"/>
    <dbReference type="EnsemblBacteria" id="AGZ42499">
        <property type="protein sequence ID" value="AGZ42499"/>
        <property type="gene ID" value="AFR_21135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4283"/>
    <dbReference type="eggNOG" id="COG4106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066439_1_0_11"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="2">
        <location>
            <begin position="52"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="30069" checksum="3DA61EFA341148C8" modified="2014-01-22" version="1">MGNHHHGHDHQHDHSTDGLPDLLDLDAEVLHAYQAEFITEVAETAGEEPRRILDLGSGTGAGTLALAARFPAAELIAVDVSPDMLERLRAKAGHAGVGGRVQTVPADLDEGWPNVDDVDLVWASASMHHVADPVRVLRNALGAIRPGGLIALVEAAGMPRFLPDSVGNGVEGRLHAALAANHAEEMPHRGADWGPLLEKAGFAVVAAPHYRINLVAPLPPATPRYAHAVLNRMRTGLHNRLSAEDRATLHTLLDGHGPETVLERDDLTVVGDRTAWIGRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W050</accession>
    <name>U5W050_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362779.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42407">
        <property type="protein sequence ID" value="AGZ42407"/>
        <property type="gene ID" value="AFR_20675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4192"/>
    <dbReference type="eggNOG" id="ENOG5032DVR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2598123_0_0_11"/>
    <dbReference type="OrthoDB" id="2179539at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="79" mass="8552" checksum="CB851CC5C6D5BC6D" modified="2014-01-22" version="1">MDDGWYRLGMLLTLAAGIWEGPIAIIFRAFTDKKPTVLTAANYLPAPWCYLAAVAAAAAAFAVIAALETRRRKATAEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0E2</accession>
    <name>U5W0E2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362931.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21445"/>
    <dbReference type="EnsemblBacteria" id="AGZ42559">
        <property type="protein sequence ID" value="AGZ42559"/>
        <property type="gene ID" value="AFR_21445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4350"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_11"/>
    <dbReference type="OrthoDB" id="1209997at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="262" mass="26752" checksum="DF7A2226C77989EA" modified="2014-01-22" version="1">MDLQLAGKRVLVTGASKGIGLAVVQAFQDEGASVVAVSRRSTPGLEKTGAEFFAADLSAPDGPQRMVEAVLAADPRLDVLVNNAGGGTMPEGALGDALDGDNDAWAEVFALNLLAAVRTTRAALPALSRARGAVVNVSSDSARRPIGVPLPYASAKAALNTFSRGLAEKYTEHGVRINVVTPSGTRTDLMESPDGFVAQLAASMNLEHGTLLAALPGQSGMLTGVLIEPSEIARAVVLLSSPTMPSAIGSNWSVDAGALKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0M0</accession>
    <name>U5W0M0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Undecaprenyl-phosphate galactose phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_16020</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16020"/>
    <dbReference type="EnsemblBacteria" id="AGZ41486">
        <property type="protein sequence ID" value="AGZ41486"/>
        <property type="gene ID" value="AFR_16020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3251"/>
    <dbReference type="eggNOG" id="COG2148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024920_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017475">
        <property type="entry name" value="EPS_sugar_tfrase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03025">
        <property type="entry name" value="EPS_sugtrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="8">
        <location>
            <begin position="257"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ41486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="452" mass="49978" checksum="65553B23D937FC85" modified="2014-01-22" version="1">MVLCAAVLALFLRFGTAGSEPYMRGYLLLTAILPVAWIACLAVNRAHEPRHLFVGTDEYARVFRSGLALTAGLAIVSFAFDLRLARGYVIIAMPLVIIVDLGARFVFRQMLHRSWARGEKLHRVILVGHERAVADMTRRLRREHHHGLGIIGACLPPGQRRQGFSPGLPPIYGTFDGVAAAVTRSDADTVVVLSCPEIDGTALRQLAWQLERDDIDLIVASTLVDVAGDRTTIRPVDGLPMLHVEHPRLKGSARVVKTVFDWVGALVLLILATPLLLTIAAVVKFAPGGRGPAIFRQERVGKNGRPFMLFKFRTMQVNAEARLTELRNLNDNDGALFKMRKDPRVTPVGRWLRRFSLDEIPQLVNVLKGDMSLVGPRPPLAREVAGYPADMRRRLVVKPGLTGLWQVSGRSDLSWEESIRLDLTYVENWSLAMDLAILARTASAVVRSSGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0M4</accession>
    <name>U5W0M4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH merR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363035.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21970"/>
    <dbReference type="EnsemblBacteria" id="AGZ42664">
        <property type="protein sequence ID" value="AGZ42664"/>
        <property type="gene ID" value="AFR_21970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4454"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110117_0_0_11"/>
    <dbReference type="OrthoDB" id="1865711at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="36"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="20754" checksum="0C68053097362F4B" modified="2014-01-22" version="1">MVGVWTMDELVERVGRALAAEYPGAPNGRVRDVPDRRAIRWYTTTGLVDRPAGMRGRTALYGPRHLLQLVAVKRRQAQGHTLAAIQAELAGATDDELQVAARVPEGLLTVGAEDADGPVRPRFWAAAPAAPSPAPPPSDAARAHPVGGVALGGGAILVLPVRPDDADYAAIATAARPLLDLLADRGLLRMNTDDGSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0T2</accession>
    <name>U5W0T2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative D,D-dipeptide transport system permease protein ddpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_22370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363119.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22370"/>
    <dbReference type="EnsemblBacteria" id="AGZ42744">
        <property type="protein sequence ID" value="AGZ42744"/>
        <property type="gene ID" value="AFR_22370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4535"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_2_11"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666261" evidence="5">
        <location>
            <begin position="22"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="270"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="95"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32481" checksum="BA4C1E94392FBE94" modified="2014-01-22" version="1">MKAVLRRVATGVVVLWAAATAAYLALLAAPGDTVDSIVGDGADTPLIRSQIITEWGLDRPAVVQYADYLWRLAHGDLGRSYLLQRPVRDIIGEQVWPTLKLALFAALLGVVLALILAVTTRRPWARRVSSTTELVLVSTPPFLIGIVLLSVFSFRFGLFPVAGDRGFAALILPAVTLALPIAGVLTQVLREGLDRALDEPFAVTARSRGLRESAVLVRHALRHALLPAVTLTGWLFGILLGGAVIIEQVFGRPGLGQVTLQAVNAKDMPVVLAVVVLSAAVYVVLNTAADLAYLLIDPRLRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W145</accession>
    <name>U5W145_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Epoxide hydrolase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_23540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363569.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23540"/>
    <dbReference type="EnsemblBacteria" id="AGZ42978">
        <property type="protein sequence ID" value="AGZ42978"/>
        <property type="gene ID" value="AFR_23540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23540"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019414_0_1_11"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EHN" evidence="4">
        <location>
            <begin position="6"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="396" mass="43518" checksum="788B037092FA684E" modified="2014-01-22" version="1">MSAAHIEPFRVRAGVGEIEDLRARLRATRWPDAPEDAGWSLGVDLGYLRELVTYWAEEFDWPAQEAALARLPRFRSRGLAFLHQRAAGPVLPLVLCHGWPDSSWRYEKVLPLLTDPGAHGADPADVFDVVVPDMPGFGHSDRPRGAPLDSIAVAGLFAELMTVLGYDRFGVAGGDIGSSVSRFLALDHPDRVVAVHRMEASMPVDLGAADLTAEERAWLDTAAAWKSGEGAYADLHRTKPQTAAVGLTDSPAGLAAWVVEKLRSWSDCDGDVEKSFTRDEILTLLTRYWLTGTIGSSMRMYAANAAIPADQHARKVEVPSGFARFPGDTLHPPRAWLERTANVVRLTDLERGGHFAPYEEPELYARELREFFRPYRGTGDQRIGLPPVTGTTAPEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W171</accession>
    <name>U5W171_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RibD_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361885.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17135"/>
    <dbReference type="EnsemblBacteria" id="AGZ41705">
        <property type="protein sequence ID" value="AGZ41705"/>
        <property type="gene ID" value="AFR_17135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3476"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_1_2_11"/>
    <dbReference type="OrthoDB" id="1920912at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="2"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="19856" checksum="AD51E627A9044DB5" modified="2014-01-22" version="1">MRKLINSTYITLDGVIENPMWTMPYFDEEAGAFAGSQTDAADALLMGRATYDGFAAAWPARDESDPSTGAAYFNNVKKYVASTTLTDPTWKNSEVLQGDLVEAVTALKAQDGKDIIQYGYGSVTTQLVRAGLVDEVRFWIHPVLEGGATLSTPLSDVKASFELIDTKVFKSGVIVASYRPAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W183</accession>
    <name>U5W183_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23725"/>
    <dbReference type="EnsemblBacteria" id="AGZ43013">
        <property type="protein sequence ID" value="AGZ43013"/>
        <property type="gene ID" value="AFR_23725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23725"/>
    <dbReference type="HOGENOM" id="CLU_1173467_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="24692" checksum="2B457A102003E725" modified="2014-01-22" version="1">MLKRQITVGSNPDSRHRRVARSDMYESATSKVQCWSAQASTATDSWAVSGWSGGRTSTIESSAIGTRSMAGSSSVPAKSVVVMMTSTSPRRRAGMLSWGSSSSRTTDRSGWRSARRATAGSARACTALWKAASVTVPAGSSASPRSSASAASIAARISRARAASRTPAAVSRTLLPVRSSSAVRVSRSSVASCCDTADGLSRPRCATARTVPWVARSASSRSRHGLRVTPRLYGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1G0</accession>
    <name>U5W1G0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase for insertion sequence element IS1086</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_23580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase IS30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42984">
        <property type="protein sequence ID" value="AGZ42984"/>
        <property type="gene ID" value="AFR_23580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4778"/>
    <dbReference type="eggNOG" id="COG2826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035706_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025246">
        <property type="entry name" value="IS30-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001598">
        <property type="entry name" value="Transposase_IS30_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13936">
        <property type="entry name" value="HTH_38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01043">
        <property type="entry name" value="TRANSPOSASE_IS30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="213"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="380" mass="42758" checksum="C726E0B08704AEDE" modified="2014-01-22" version="1">MAQGMNNSEACRIVGVGRGTGIYWRYGRTVKNPDGSTRTYSPIMASVQISERFLSEDERVAIADQYRAGASIRGIATALGRQPSTISREIKRNSDPETGKYHPFRAQKRARARRARPKAGKLAADSSLRLFVQQRLADRWSPEQISNVLPGLFPDQPAMRICHETIYQALYRPDHPLTRAAPRRPLRSGRLKRRRRRRADQRTTHFVEAGHAISLRPAEANDRSVAGHWEGDLVLGKNNKSAIGTLIERSTRYAVLIYLPEGHNAGQVRDALIKLFRKLPAGLARTLTWDQGGEMGRHHEFSTATGVPVYFCLPGRPWQRGSNENFNGLLRQYFPKGTDLGAYDAEHLAQIAKQLNDRPRRCLGWTTPAQQLAKLFPAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Y6</accession>
    <name>U5W1Y6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_24860</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3 4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363828.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24860"/>
    <dbReference type="EnsemblBacteria" id="AGZ43238">
        <property type="protein sequence ID" value="AGZ43238"/>
        <property type="gene ID" value="AFR_24860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5036"/>
    <dbReference type="eggNOG" id="COG0545">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013615_2_0_11"/>
    <dbReference type="OrthoDB" id="1861282at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="115"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="202" mass="20384" checksum="3CF331A7B378D57C" modified="2014-01-22" version="1">MSVQDKTGTSRRGQALTGAFAGVAVIAVLVAVYIGIQVSDDDETTPAAAPAASAPAQPPAEAPAEPAPSQPAPAAPAQVDPALQKPPVIKAGKGAVKELLVTPVVAGKGPKVQAGQTITANYVGVTYKDGKEFDSSWKTGQPVQFPVGAGQLIEGWDKGLVGVPVGSRVQLDIPAAMAYGEQAQGGRPSGDLRFVVDVLAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W219</accession>
    <name>U5W219_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbonic anhydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible hydration of carbon dioxide to form bicarbonate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363749.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24465"/>
    <dbReference type="EnsemblBacteria" id="AGZ43159">
        <property type="protein sequence ID" value="AGZ43159"/>
        <property type="gene ID" value="AFR_24465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4955"/>
    <dbReference type="eggNOG" id="COG0288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084253_1_2_11"/>
    <dbReference type="OrthoDB" id="1841447at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175">
        <property type="entry name" value="PTHR43175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="SSF53056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="17777" checksum="E1F7DDB1D5012E65" modified="2014-01-22" version="1">MSVTDELLANAERYAAGFDNGALPLPPAKHVAIVACMDARLNPYGLLGLSEGDAHVIRNAGGVVTADQLRSLAISQRLLGTTEIVLIHHTDCGMLTFTDDGFKESIRQEVGSKPPWAAEAFADLDDDVRQSVRRVLDDPYIPVKDSVRGFVYEVETGRLREVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W224</accession>
    <name>U5W224_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24515"/>
    <dbReference type="EnsemblBacteria" id="AGZ43169">
        <property type="protein sequence ID" value="AGZ43169"/>
        <property type="gene ID" value="AFR_24515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4965"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_769123_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="348" mass="37671" checksum="26FB15D6D9694582" modified="2014-01-22" version="1">MTSFAVVGAGWRAEMFWRLAGGLEGLDCVGAVVRSPRELPVPTFASLAECVAEAAPDFVVTAVPWNVSPGLIVEAVERGLPVLAETPPAPDVAGLRSLWSAVGESGLVQVAEQYLLMPSHAARLAAVRLGLIGTPTQVQVSSTHQYHAVSMIRGILEAGRGPVTVRASRTTAPLVDPLKRDAWTDDPDPKPATTTIATLDFGEGLSGVYDFTDNQWHNQLRFRRLLVRGSHGELRDDEVVRLTEPRTIVRTPLVRRQTGYDLDLDGFDTDTITYGGEVLYRNPYPGRRWMDEEIAIATLLDATAAWVRGTAPAPYPLADGTQDHLIALAVEEAADTDRTVTTGSEPWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W266</accession>
    <name>U5W266_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Beta-galactosidase</fullName>
            <shortName evidence="5">Beta-gal</shortName>
            <ecNumber evidence="3 5">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_18880</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyl hydrolase 42 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3 5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362423.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18880"/>
    <dbReference type="EnsemblBacteria" id="AGZ42050">
        <property type="protein sequence ID" value="AGZ42050"/>
        <property type="gene ID" value="AFR_18880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3835"/>
    <dbReference type="eggNOG" id="COG1874">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012430_1_1_11"/>
    <dbReference type="OrthoDB" id="831762at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009341">
        <property type="term" value="C:beta-galactosidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013738">
        <property type="entry name" value="Beta_galactosidase_Trimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003476">
        <property type="entry name" value="Glyco_hydro_42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013529">
        <property type="entry name" value="Glyco_hydro_42_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36447">
        <property type="entry name" value="PTHR36447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02449">
        <property type="entry name" value="Glyco_hydro_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08532">
        <property type="entry name" value="Glyco_hydro_42M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001084">
        <property type="entry name" value="B-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_42" evidence="8">
        <location>
            <begin position="24"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_42M" evidence="9">
        <location>
            <begin position="406"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001084-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001084-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ42050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="666" mass="72984" checksum="53DD38E9D06B06F3" modified="2014-01-22" version="1">METSHRWLRPTDPRAGRRLEFGADYNPEQWPRSVWDDDIRAMRVAGVTVVSLAIFSWARLEPSEGEYTFGWLDEVMDLLHAGGIAVDLATATASPPPWLTTKYPEILPVDRQGATVWPGGRQHWRPTSPIFREHALRLVRTMAGRYGGHPALTAWHISNELGCHNAYDYSDDAAAAFRTWLQNRYTGLDALNAAWGTAFWSQHYSEWAQILPPRQAATHPNPTQQLDFKRFSSDALKDYLRAERAVLRELTPEVPATTNFMVMDSTKALDYADWAAEVDFVANDHYVQRGPRPLDELSFSANLTGHLAGGRPWFLMEHSTSAVNWQPINVPKAPGALARDSLTHVAHGADAVCFFQWRQSVAGAEKYHSAMLPQAGEDSAVFRSVVQLGETLRRLAPLSGTPRSPARAAILFDWTSWWVAEQDSHPTDRLRYRQEALDWYSAFLDLGVRADVVPVATDLSGYDLVVAPVLHVVPAALAERLTAFVAGGGHLVTTYFSGIVDENDHLWAGGHPGAFRDLLGLRIEEFGPLPDDEPAELDNGSAGTLWADHPTVTGTDVTVLAAYRDGSPAVTRRTAGTGSAAYVGTRLGPDGLAPVLGELLSLAGVGSELPPELRGRVELAIRGDFRFLINRTAGPVELTGLAGEALHGSATLLEARGVTVLRVDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2D2</accession>
    <name>U5W2D2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MCP methyltransferase CheR with TPR domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364061.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25670"/>
    <dbReference type="EnsemblBacteria" id="AGZ43398">
        <property type="protein sequence ID" value="AGZ43398"/>
        <property type="gene ID" value="AFR_25670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5203"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025854_4_1_11"/>
    <dbReference type="OrthoDB" id="1378151at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="465" mass="52805" checksum="370C6B1AA20ADC99" modified="2014-01-22" version="1">MTATPEVTRFRTLLAAKLGWTFDDNDVSQLDDVLAKRSGSHRLSRTAYLSRLGAPGWDAELTKLAEDLTINETYFYRHGEQFRALADVALPERLQARSAQRVLRMLSVGCSSGEEAYTLAIVARESQPDRDWIVSVLGLDASPAVLRRATAARYSSWSLRETPDDVRQRWFHPHDGYYELDAAIRATVQFRQYNVATPDDNLWRPDQYDVVFCRNLLMYLTQPVRDQLIERMTRSLSPGGYLFLGHTDSLGSRPEGLEPQHTHQTFYYRRRTVPDALRFEEPLVVTPTRTRPAPQEPIESDAYERALRLLHDERFGEALKLIEASPQDRPAPRDLLLHSVLLAHTGRIDEGERLCRRLLDLDGLYADAHHQLAVCLEDGAGTDAAIAQYRLAAHLDPEFAMPRMRLGLLARRRGENRTAATELDRALTLLRQERDDRIALFGGGFGRIALTTLCRTELDACGARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2D8</accession>
    <name>U5W2D8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Multi-sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_25695</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25695"/>
    <dbReference type="EnsemblBacteria" id="AGZ43403">
        <property type="protein sequence ID" value="AGZ43403"/>
        <property type="gene ID" value="AFR_25695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5208"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_377517_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="14"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="142"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="278"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="448"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="663" mass="72484" checksum="19274D2D6DD3FEF6" modified="2014-01-22" version="1">MTQHPTGTPMVRHTVLIVDDSLTVRMDLHEAFEADGFVSILCATGAEARAAFAEAHFDVAVLDVLLPDADGVELLRELRAHPGREGTVTVLLSSEAEIADRLHGLRTGADEYVGKPYDAGYVVARARQLLGEDPSRADDRTTVLVIDDSMTFREQLRELLEPEGYAVITANSGEEGLRTAADRRPQAVIVDGIMPGIDGATVIRRLRLDPALRDIPCLLMTAADDYATEVQMLEAGADAFVRKQQDLAVVLAKLAAVLRTSAAQLPIEVTGSLHGPGKVLTVTADRQRLSALGDALREDGYDVVATGNGSDALDLLAAQPVDCIVLSLDSSGVDARETCRRIKEVPQIGDTPVIMIGDHDDALLDCLAAGADDYVRSADGPETLRAHVRGQIRRKQSLDESRRIREELMRRELDAAGERAARQLAETRAALVEELEWRNRELEAFSGSVSHDLRGPLQIISSFAETMLDEDEEPLSSQARHRVQRIHAAAERMADLVESLLILARASRGDLRRQRFDLTATTWQVIREVEARDPGRDVRFTVAEGMTADGDEGLVRVILENLINNAFKFTRKIEKPVIEISWSGPEENPRYSISDNGAGFPAGKAIELFRPFARLHSADDFPGTGIGLTTVHRAVERHGGEIHAEGEDGNGATFWFTLPPARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2R1</accession>
    <name>U5W2R1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_19990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19990"/>
    <dbReference type="EnsemblBacteria" id="AGZ42270">
        <property type="protein sequence ID" value="AGZ42270"/>
        <property type="gene ID" value="AFR_19990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4056"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="124"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="20592" checksum="05EC5372143A61B6" modified="2014-01-22" version="1">MTDVSVVSSESSERALPLTGRAARDAEFTAFVAASAPRLRRLAYLMCRDWHLAQDLTQTTLAKMYASWSRIWRTANLEAYSRKVLMNTLLDQKKRRSDSEVVLAELPEPPARPAGSTRELSVTLLEALATLPVHDQAVVVLRHWEDHSVDTVAEILGVTPSAVKMRNARALSRLRALLGDDFPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W329</accession>
    <name>U5W329_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isocitrate lyase</fullName>
            <ecNumber evidence="1">4.1.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Isocitrase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Isocitratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_25790</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-threo-isocitrate = glyoxylate + succinate</text>
            <dbReference type="Rhea" id="RHEA:13245"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="4.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Can also use Mn(2+) ion.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.1" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364109.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25790"/>
    <dbReference type="EnsemblBacteria" id="AGZ43422">
        <property type="protein sequence ID" value="AGZ43422"/>
        <property type="gene ID" value="AFR_25790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5228"/>
    <dbReference type="eggNOG" id="COG2224">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019214_2_0_11"/>
    <dbReference type="OrthoDB" id="152545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
        <property type="entry name" value="PTHR21631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00463">
        <property type="entry name" value="ICL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
        <property type="entry name" value="Isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01346">
        <property type="entry name" value="isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="194"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="315"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="429" mass="46422" checksum="094039E18D4C6712" modified="2014-01-22" version="1">MTDQLTEVRGGTAADLTRAWAGDPRWLGVTRSYTAHDVVKLRGSVQEQHTLAERGARRLWELLHDEEYINALGAMTGGQAVQQVRAGLKAIYLSGWQVAADANLSGHTYPDQSLYPANSVPAVVRRINNALLRADQIDTAAGKHDRDWLAPIVADAEAGFGGPLNAFELMKAMIVAGAAGVHWEDQLASEKKCGHLGGKVLVPTAQHIRTLNAARLAADVAGVPSLIIARTDALAADLLTTDVDERDQPFLTGERTAEGFYRVRSGPEAAIARGVAYADYADLIWCETGKPDLEFARSFAEAVHKVHPDKMLAYNCSPSFNWKRNLDDATIARFQKELGAMGYKFQFVTLAGFHALNHSMFELSKGYASDGMSAYVKLQEAEFAAETDGYTATKHQAEVGTGYFDAVSTALNPESSTTALSGSTEAEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W338</accession>
    <name>U5W338_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362782.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20690"/>
    <dbReference type="EnsemblBacteria" id="AGZ42410">
        <property type="protein sequence ID" value="AGZ42410"/>
        <property type="gene ID" value="AFR_20690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4195"/>
    <dbReference type="eggNOG" id="COG1830">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056329_0_0_11"/>
    <dbReference type="OrthoDB" id="1564925at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="30184" checksum="88B6E3C21F8E66E9" modified="2014-01-22" version="1">MRDVLEIVAARADRPGAIASAAAARRRPAGWRGEHGRMMIIAADHPARGALRAGPDPLAMGNRADLLDRICTALERPGVNGVLGTPDILEDLLLLGALDDKVVIGSMNRGGLAGTSFEIDDRFTAYDGDSLAAAGFEGGKMLLRIDPDDAGTVATLQACGRAVGDLAAHQLMAMVEPFISHRADGRVRNELSPEAMIRAMTVAAGLGTTSAYTWLKVPVVDDMERVMAATTLPALILGGEVSQDADAAYVRWSKALALPTVQGLVIGRSLLYPPGGDVAGAVDRAVSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3A6</accession>
    <name>U5W3A6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26610"/>
    <dbReference type="EnsemblBacteria" id="AGZ43584">
        <property type="protein sequence ID" value="AGZ43584"/>
        <property type="gene ID" value="AFR_26610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5393"/>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061789_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="50"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="270" mass="28995" checksum="D900A721C0F74DAE" modified="2014-01-22" version="1">MVLMLSPEILEYYQRGGEQERLTAGAGRLEFLRTWDVLTRTLPPAPARILDVGGATGIYARPLAEAGHSVRVIDPVPEHVTTSGALDGVTAAVGDARDLPEADGSADAVLLLGPLYHLGDRADRLTAWREAARVVRPGGVVVAAVISRYASMLDGILKGFFADPAYVSMVDRTLTDGVHSHRGQVPRWFTSAYFHHPDEPAAEAAEAGLTAARTVAVEGPAWLTGPRLLEFLDDDHLRGVMLDHLRRVEAEPSLLGASSHLLTVAHRPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3I1</accession>
    <name>U5W3I1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonate-semialdehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_27560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560111.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27560"/>
    <dbReference type="EnsemblBacteria" id="AGZ43773">
        <property type="protein sequence ID" value="AGZ43773"/>
        <property type="gene ID" value="AFR_27560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5586"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_10_11"/>
    <dbReference type="OrthoDB" id="490746at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="PTHR43866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="3">
        <location>
            <begin position="15"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="494" mass="52199" checksum="C28E8FE1226325DF" modified="2014-01-22" version="1">MREIKHWIDGTAVEGTSGRQLPVWDPATGEQQAYVVAATADETAKAVASAKAAFPAWRAASLSRRAEVMFRFRELVDANRKEIASLVSQEHGKTVADAGGELARGLENIEFAAGAPHLLKGGYSEQASSGVDVYSIRQPLGVVAGITPFNFPAMVPMWMFCNALVCGNTFILKPSEKDPSVSLMLADLLRQAGLPDGAFNVVQGDREAVETLIAHPDVQALSFVGSTPVARIIYETATAKGKRVQALGGAKNHMVVLPDADVNAAADAAVSAGYGSAGERCMAVSVVVAVGSVGDDLVAAISDRIGRIAVGPASDPAAEMGPLISREHRDKVAGYLADAGTAEVVVDGRDAPISDGPGFFLGASLVDKVPTDSPLYTDEIFGPVLSVVRTETYEEALDLVNTNEYGNGTAIFTRDGGAARRFQYDVNCGMVGVNVPVPVPVAYYSFGGWKSSLFGDLHMYGPEGLQFYTRSKVVTSRWPDPATSEIDLGFPKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W435</accession>
    <name>U5W435_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AP_endonuc_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560120.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27605"/>
    <dbReference type="EnsemblBacteria" id="AGZ43782">
        <property type="protein sequence ID" value="AGZ43782"/>
        <property type="gene ID" value="AFR_27605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5595"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059523_1_0_11"/>
    <dbReference type="OrthoDB" id="1194699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666061" description="AP_endonuc_2 domain-containing protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="AP_endonuc_2" evidence="2">
        <location>
            <begin position="70"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="308" mass="33101" checksum="3A86B91B0D71A022" modified="2014-01-22" version="1">MQNESTVSRRNLLGAAAAGAAAIGTSAVLASPAEAGGRGGHHPSRVPRDKISVQLYTLRDQLNIDLDASLAELAEIGYKRVEHAGFVGRTAAQFRAALDRAGLKATSGHVGIPQPFNASTWQKALEDALIVGNKYIVHPYFGGAADGGPIVDGSVYKAFAKDLNKAGALARKAGLSFGYHNHHNEFARQDGGKRTGFDILTGETDPGLVHLEVDLYWAWRGANDPVDLIHRHKGRIRQVHVKDMATSSSFADPGTGLIDFGRIFEHSREAGLVEYIVERDDAGSPPRTPADALVTAEVGFRYLDNLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4J3</accession>
    <name>U5W4J3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22905"/>
    <dbReference type="EnsemblBacteria" id="AGZ42851">
        <property type="protein sequence ID" value="AGZ42851"/>
        <property type="gene ID" value="AFR_22905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4642"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_39_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="295" mass="31911" checksum="E743C27B34652B61" modified="2014-01-22" version="1">MDLNLLTALDALLDERSVTGAAERLRLSPPAMSRTLSRIRKATGDQILVRTGRTMTPTPHALTLQAEVHDLVQRAETALRPERPLDLGTLERTFTVRGHDAVTTALGPELLGIVRERAPGVAVRLLAEASADTNELRHGQVDLEIGSTAPELPEITGERIGRDRLVVAMRTGHPLASGALTLEDFAAADHLTVSRRGRLTDPMDDILSGHGLRRRVVASAPTSTTALAIAARSDMLVAVPGAACAAMIDALRLTTLELPFTVPPVDLIVLWHQRFSNDKAHAWLRACVREAFERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4P0</accession>
    <name>U5W4P0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560314.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28580"/>
    <dbReference type="EnsemblBacteria" id="AGZ43977">
        <property type="protein sequence ID" value="AGZ43977"/>
        <property type="gene ID" value="AFR_28580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5795"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_27_3_11"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="56"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31462" checksum="4DCEEAA7BF524F0E" modified="2014-01-22" version="1">MPIGEFRSDQARDRFLTVYDDAVHRLWPSSSVDSDVVTSFGTTRVHRLGSGDRTPFVLLPGSGGPSLMWHDYVGRLAADRPVITIDPVGEPGPSVQTRAITSGDEWSCWLGEVLAGLGVGRAHLVGTSYGGWIALRHALRSPQAVAGLVLLDPGGFGKVSRKFVAWIIACGLTGFAPRPVRHALARPLRNASLREDDLMPLVKASFGFRRKHIPPTPLTDDDLERITAPTLLLVGGHSQLYDARAVATRVTTRMPHAVAEVIPEAGHDLVLWHPDELAARMVAFAADASAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4P8</accession>
    <name>U5W4P8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363434.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23205"/>
    <dbReference type="EnsemblBacteria" id="AGZ42911">
        <property type="protein sequence ID" value="AGZ42911"/>
        <property type="gene ID" value="AFR_23205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4703"/>
    <dbReference type="HOGENOM" id="CLU_2233416_0_0_11"/>
    <dbReference type="OrthoDB" id="2069110at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="13216" checksum="D619E71EB2A6A028" modified="2014-01-22" version="1">MGEFDYYDGKILHVAGDAIRAEGDKWKDMSGDMAKVAETTKTLWLTPGAFMVADPWAGPAVSVDQFKIYDDVHQLLCSLFDGATAEFAQLDKALHKLAKEYEESDERTYINISDIYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W502</accession>
    <name>U5W502_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560551.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29765"/>
    <dbReference type="EnsemblBacteria" id="AGZ44214">
        <property type="protein sequence ID" value="AGZ44214"/>
        <property type="gene ID" value="AFR_29765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6031"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_2_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="22249" checksum="7FEA2AAF3594C3BD" modified="2014-01-22" version="1">MIESAGLRARKKLRTRDAIADAAISLFLARGFDQVSVSDVAAAAEVSKPTLFRYFPTKEDLVLHRFADHQGEAARVVSGRHPDVSPMAALHSHFRAGLDRFEPVTGLNDHPEVVAFHQLVFATASLTGRLTQYLLDDEDALAAALGHGITGRLQAAQVIAVQRVLARTNWQKIADGRTAHEVRPEAIADAEQAFARLRHEEGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W592</accession>
    <name>U5W592_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363666.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43076">
        <property type="protein sequence ID" value="AGZ43076"/>
        <property type="gene ID" value="AFR_24040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4870"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_0_11"/>
    <dbReference type="OrthoDB" id="968232at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24172" checksum="4CE84A8CEE5F7A28" modified="2014-01-22" version="1">MTTTLITGGNKSLGFETARRLHEHGHHVIIGARDADRGAQAAKDLGVDWVTLDVTSDESVAAAASEVRARFGGLDVLVNNAGVSGQITAVENFDGPEVLAVLDTNTVGIVRTTHAFLPLLRESAAPVIVNVTSGLGSFSVRADESRIEHNVPSLGYSASKAAVNMLTLMYAQFLPGIRINVVDPGYTATDFNGHSGHQTVTEGTDAIVALATIDKAGPTGTYSDRHGPVGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5A1</accession>
    <name>U5W5A1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560661.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30320"/>
    <dbReference type="EnsemblBacteria" id="AGZ44324">
        <property type="protein sequence ID" value="AGZ44324"/>
        <property type="gene ID" value="AFR_30320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6139"/>
    <dbReference type="HOGENOM" id="CLU_2646355_0_0_11"/>
    <dbReference type="OrthoDB" id="2178642at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="77" mass="8503" checksum="0D23FEE5A09F7C09" modified="2014-01-22" version="1">MADKDLLRIRETALAYAEAVRTTQRFFDRVEDLDDPSVQIEYATLVEREKEAAGARLDALESTGIEVSSVDGSDPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5X6</accession>
    <name>U5W5X6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alkyl hydroperoxide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_25435</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363967.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25435"/>
    <dbReference type="EnsemblBacteria" id="AGZ43351">
        <property type="protein sequence ID" value="AGZ43351"/>
        <property type="gene ID" value="AFR_25435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5153"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_1_11"/>
    <dbReference type="OrthoDB" id="1996723at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="3"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="3">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="16972" checksum="72636F2CB6068B06" modified="2014-01-22" version="1">MSIDKGDQAPDFELPDQTGTPRRLSTLLADGPVVLFFYPAAMTSGCTAEACNFRDLAAEYAQAGAQRVGISRDPVEKQKKFADLNGFDYPLLSDPASSAAEAFGIKRKLPLGPLSVKRVTFVIDTDRTVLEVIHSERDMNEHADRALDVLKARSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W646</accession>
    <name>U5W646_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lipid A biosynthesis lauroyl acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_31110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31110"/>
    <dbReference type="EnsemblBacteria" id="AGZ44482">
        <property type="protein sequence ID" value="AGZ44482"/>
        <property type="gene ID" value="AFR_31110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6297"/>
    <dbReference type="eggNOG" id="COG1560">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049421_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07984">
        <property type="entry name" value="LPLAT_LABLAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004960">
        <property type="entry name" value="LipA_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30606">
        <property type="entry name" value="PTHR30606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03279">
        <property type="entry name" value="Lip_A_acyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="33477" checksum="AFCC7AB8B157786C" modified="2014-01-22" version="1">MSSRRDQLTDLGYAAGWRVVRMLPLPAARAIFNAGADRSFKGNGPSVQRLRRNLRTVVGPDLPEAGLDALVKAGLRSYARYWMEAFRLPSQSREDFLTDFHLFDADEFNAVLKEGNGAILALPHVGNWDAAAAWVTAQGWPLVTVAERLKPESVFERFVAYREKLGMEVLPLTGGQRPPLDVLAERATQGYAVALLADRDLSSRGIEVQFFGGRTRMPGGPAMLALRTGAPLYAADLWFEPSRTVGRIRRIELPEKSEGALDVRVKLTTQRLADAFALGIAEHPQDWHMLQKLWLDQSTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6S9</accession>
    <name>U5W6S9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26825"/>
    <dbReference type="EnsemblBacteria" id="AGZ43626">
        <property type="protein sequence ID" value="AGZ43626"/>
        <property type="gene ID" value="AFR_26825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5436"/>
    <dbReference type="eggNOG" id="COG2718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052813_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="SSF48613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="321" mass="35481" checksum="93AFAF097277823E" modified="2014-01-22" version="1">MKLPSARGPLSAEVIDALAHPPHDLPTLPDAAGDEDLHLTLFVLYELHYRGWDGVDDHWEWHPGLIALRAGLETRFEADLRDLAGEIPATGDVPHALIAMAGETGGPSLSGYLRGRATREQFCEFAQHRSVYHLREADPHTFAIPRLAGKAKAALIEIQIDEYGGGAANRMHQEMFRDTMTELGLDTTYGAYVDVVPWQTLANNNLMSLFGLHRRWRGALLGHLAGYEMTSTGPNRAYGNALRRLGGSETATRFYDEHVEADAVHEQIAAYDMCGSFCRAEPALAADVLFGARCVLAMEDRWAAYVLERWAAGESSLRWTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W782</accession>
    <name>U5W782_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33900"/>
    <dbReference type="EnsemblBacteria" id="AGZ45038">
        <property type="protein sequence ID" value="AGZ45038"/>
        <property type="gene ID" value="AFR_33900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6862"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_7_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019921">
        <property type="entry name" value="Lucif-like_OxRdtase_Rv2161c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03619">
        <property type="entry name" value="F420_Rv2161c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="313" mass="33484" checksum="72312F11C1088D35" modified="2014-01-22" version="1">MAGNRERQYGVRMLISCGCPVSGVWADPLSVTGFAMRAEELGYHTLWSFQRLLAGADQNLAPVYQSVLDPLLALTYAAARTTQIRLGVAVINLPYVSPTYLAKQASTLDVLSGGRFDLGLGTGWSEVEFAATGSDPNPRGRRTEEYLAVLRTLFRGGQVSHAGEFYTVPPSVMAPPPVQAGGPPILLGGTADVALRRAGRIADGWVSSSKASLDDIARGTQIVRRAAEESGRDPDSVRIIVRGVVQAGLRDDNVPLSGTWDQIRGGARLYEEAGVTELFYDLNWDPFIGSPDGDPRAAGERAEEILTALTPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7G2</accession>
    <name>U5W7G2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28150"/>
    <dbReference type="EnsemblBacteria" id="AGZ43891">
        <property type="protein sequence ID" value="AGZ43891"/>
        <property type="gene ID" value="AFR_28150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5706"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_588979_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="468" mass="50508" checksum="1B504CC362F5F4F4" modified="2014-01-22" version="1">MTQAELAYRRLEPITLGSHREFLERRLAKIGAPVPRYPSMFDSPLHEALGEVTADVLAALVDLGFAPGRVAALTVGLTGNDGFTARYRSFPGDGSGLVLVSDSMVSLCYSYCEFEGRALQAMVSGGLLRMAAKVFLTRFTGTIGGDPRSLAGILRYHNVNRRVYGVPAVLNLVPVPDDPGMDEFLLDYGLRFVVGHEAAHHVLDDGHSELDADTLALFTAAEAFARYQAENMDFLPERYRREAAEFHGLIGALITMLALQTSERALFLRRGHSHLPAQDRATRLVEHVLSPARQETLDRLLGGRDPRMALRFDKDRGNLGVYVGALVRATAQASDFSAGATPFDWAALAGVPEIEQLAGADLDEIAALDRLMCGPDPVLTDRLDPGTRHILAGRLKEGLSAWGLAADRVEALLDPSRVLAFHTLVEDLQNTAKDLHTAVAAATLVARRLPGTESSSPGAGVPMGGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7P1</accession>
    <name>U5W7P1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28745</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560347.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28745"/>
    <dbReference type="EnsemblBacteria" id="AGZ44010">
        <property type="protein sequence ID" value="AGZ44010"/>
        <property type="gene ID" value="AFR_28745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5827"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_3_11"/>
    <dbReference type="OrthoDB" id="787596at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ADH_N" evidence="4">
        <location>
            <begin position="25"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="ADH_zinc_N" evidence="3">
        <location>
            <begin position="188"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="382" mass="41470" checksum="28E6045CF425916C" modified="2014-01-22" version="1">MQAMVYRGPYRVRVEEKDRPSIEHPNDAIVRVTRAAICGSDLHLYHGMMPDTRVGMTFGHEFVGVVDEVGPSVEHLRPGDRVMVPFNIFCGTCFYCARGMYANCHNVNPNATAVGGIYGYSHTTGGYDGAQAEYVRVPFADVGPSLLPDWMDEDDAVLLTDAVATGYFGAQLGDITEGDVVVVFGAGPVGLYAAKSAWLMGAGRVIVIDHLDYRLAKAAEFAHAETCNFTEYDDIVVHLKKITGYLGADVAIDAVGAEADGNLLQHVTAAKLKRQGGSPVALNWAIDSVRKGATISVMGAYGPIFSAIKFGDAMNKGLTLRMNQCPVKRQWPRLFEHVKAGYLKPSEIITHRIPLEHIAEGYHIFSAKLDDCIKPIIVPNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7R3</accession>
    <name>U5W7R3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28650"/>
    <dbReference type="EnsemblBacteria" id="AGZ43991">
        <property type="protein sequence ID" value="AGZ43991"/>
        <property type="gene ID" value="AFR_28650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5808"/>
    <dbReference type="eggNOG" id="ENOG50349BY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157213_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="82" mass="9568" checksum="B9C7ADA1BD1A0AA1" modified="2014-01-22" version="1">MIAEYHETELGKLVARFGEAVDRYRAGELDAFEVDQVAFQYSRAAKELWKYCNLLQAEIAAAIIREDPPRDWWDRGAPRRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W875</accession>
    <name>U5W875_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CRISPR-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560512.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29570"/>
    <dbReference type="EnsemblBacteria" id="AGZ44175">
        <property type="protein sequence ID" value="AGZ44175"/>
        <property type="gene ID" value="AFR_29570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5994"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045222_0_0_11"/>
    <dbReference type="OrthoDB" id="1030744at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014082">
        <property type="entry name" value="CRISPR-assoc_prot_Cas02710"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02710">
        <property type="entry name" value="TIGR02710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="477" mass="52608" checksum="0BDE2275BC1D10A8" modified="2014-01-22" version="1">MGSLDEKHSRMRRIFRGEEEYGGGTAAEQAQAFRLAEIYDEAVEVAQANSADVERPEVDLLISLSGFSPETTLLAFALTRPTRILIITSEGTQKTIDTIWEKLAGKIKFSEARHVTCDPVDPTSIYDIVLKEVRSLLAAGRPPRVIIDITGGKKAMSAGAALAASQLDLPMCYIDSTFDPEMRQALPGSERLCVLPNPTALFGDKDLTAAMAMFGSGVYSGAHALFEKLSDSIAEPTQVRFLRDLAALYEAWCNLDVDGLPELIARVREHLRENRMALAVPVTQRIAKQLEFAEALAGRDGPTMLLNFFLLGEHYRAQGRHDFAALLYYRSIEKVFEERLSSEFGLDPVDPDYTKLGDVEDLTTRYAALTTEVYGAPTDTLPRKIALMDAMLLLCLKDDAVLKRIGWTTPSSISSMRGVVDTRNRSVLAHGTASVSMDQSAQLSGRAGALLRYFLQVHAPNQDITERIKTLRFVSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8B1</accession>
    <name>U5W8B1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_29500</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560498.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29500"/>
    <dbReference type="EnsemblBacteria" id="AGZ44161">
        <property type="protein sequence ID" value="AGZ44161"/>
        <property type="gene ID" value="AFR_29500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5979"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_11_1_11"/>
    <dbReference type="OrthoDB" id="498436at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="15"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="541" mass="56722" checksum="A46A55CED7EDA500" modified="2014-01-22" version="1">MTTVAGTWAPLRHPVFRAVWIAAFVANVGTWMQTVAGQWLLIEQHSSSLLISLVQSASSVPVLLLVIPAGMVADFLDRRRLLLVTQGVQAVLAGILALLTAAGRTSPEVLLLFTFLLGCGAAAQMPAFQAMVSDLLPRSELGPGASLSSLGVNLARAVGPAIAGLLLAHLGVPWLFGLNAISFVLFGAALLLTPAGRPGTGSAVKAASLSSFEAGGRYVLNSPAVRRILLRLVLFALPANVLWALLAPLAEGRLGLGAAGYGVLLGAAGAGAVAGAVLMPLVQNRLSASAKLTVAGAVYGAGLIGLALTRNVGVAVALLVPIGVGWIAVIAGLNAGVQAFLPDWVRARALAVYQMVLFATFAGSAALWGAVATWIGLGRTFAGAGILLVATTVAGVWLPLRRTDIGDRTAVPPGVLPEATFVGAITPDDPVEVLVRYHVRPERRDDFVRAIAELRTSRLRTGATQWVLLSDAVETDQLVERYRVASWTELLDQRSNRQTPFDEEVRRRVEALADTVTQQHLLNVAVPHHVHNVVQHLGEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W986</accession>
    <name>U5W986_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562034.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37225"/>
    <dbReference type="EnsemblBacteria" id="AGZ45699">
        <property type="protein sequence ID" value="AGZ45699"/>
        <property type="gene ID" value="AFR_37225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7531"/>
    <dbReference type="eggNOG" id="COG2268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031020_0_0_11"/>
    <dbReference type="OrthoDB" id="1442240at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="411"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="29"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="228"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="449"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="516" mass="54989" checksum="EF3352F39C63837B" modified="2014-01-22" version="1">MSHGGEIFGLGGDAASEPSFETALRGYEKKQVERYVARAENEIAALASEREQAYSQIQAMAGQIDRLQQEANQARRRPGMGAEVSFRHLGPKVEEILAKAEEVAADIKSSATDDIAARLAEAERIRAEAEAHAHDGIRDFEIALAARRADEEKADVARRAAAEKSLATSRQAAEQLRAEAESTLARARSEAQQLTERTAQESQRARAEADGYVKSTRMQADQELKALRDKTQQDVAKLRADAERERAELKATVDREVAQRRQELTQELTQMRSAVEKQCADLRSEADKYAEEVLRRSDEQATAIRKEIAAQQDKIAGAGRDLAAAQAKTAEAEKKVAEANARAKAAETEAERMQQRLADVKKELEAELVHVAEAKRAGEAAERRATDVRRQVQKEAKRVAELAAAAVLAAAASPDGEDDYDDEDLSSPSVAAAAASVAPVATGHAAVVPPSSAPQTTTSGGGQHAAPEKASTTTDKAATPASGPATPAAAAAPAKVTASAKVTARPTDSRVGVDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9V4</accession>
    <name>U5W9V4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_37740</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562137.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37740"/>
    <dbReference type="EnsemblBacteria" id="AGZ45802">
        <property type="protein sequence ID" value="AGZ45802"/>
        <property type="gene ID" value="AFR_37740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7632"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="19"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="105"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="19212" checksum="F358B7E66A7C9DA2" modified="2014-01-22" version="1">MANGSDAEYTAYVHGRATALRRTAYLLCGDEHQADDLVQETITKLYARWPKISRQAENLDSYVHTILVRIFLDEKRRGWWKVRLGIPPDRPAPVVPAHDVEERAVLRTALSQVPPRQQAVLVLRFLCDQPIADVARVLGCSEGTVKSQTAHGLTALRRILGDRIHTLGSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Z5</accession>
    <name>U5W9Z5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38845"/>
    <dbReference type="EnsemblBacteria" id="AGZ46023">
        <property type="protein sequence ID" value="AGZ46023"/>
        <property type="gene ID" value="AFR_38845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7860"/>
    <dbReference type="eggNOG" id="ENOG5032BG9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108347_0_0_11"/>
    <dbReference type="OrthoDB" id="1989433at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="20558" checksum="6FFC853BA22FAC70" modified="2014-01-22" version="1">MERRWLGLDRRSLLPALVVLVIAVVLRAVIPWIDHHIAEDDVVRAGDRLNLSSGLTVTPPVGWLLIDGLLVGASTVQPGTGSSTATVARSGVGAQIQVAPFAGDANALLDQLNRNESRSDDRPEFTIDGSRGAVNAAGGIAGVVENYTSTSSDGIIAAYVLPDGRGLAIEVSGAESQLSAHNGQISAMLRSVSVEARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA24</accession>
    <name>U5WA24_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45994">
        <property type="protein sequence ID" value="AGZ45994"/>
        <property type="gene ID" value="AFR_38700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7831"/>
    <dbReference type="eggNOG" id="ENOG503390F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118435_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="22232" checksum="4D566330A82091E8" modified="2014-01-22" version="1">MFRRDGEGGFGEGEASEGTHAFPGRTIEVDLEDEPTTDLRSRSKRGWLRRIDGRTRSILTGAAVAAIIVNAGAVWAYWQITGSETGRDTAGTVVELNLRARSDLNKPLVPGGAGNLTVTVTNDNDFPIRITSVAPGEGNIIADDEHREAGCTTTGVVVAQRSVKVEWDVPRNTIGAFTVPDGLAMGKGSDKACKGATFTVPVLVSGLAGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA46</accession>
    <name>U5WA46_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fumarate reductase iron-sulfur subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_38825</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562353.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38825"/>
    <dbReference type="EnsemblBacteria" id="AGZ46019">
        <property type="protein sequence ID" value="AGZ46019"/>
        <property type="gene ID" value="AFR_38825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7856"/>
    <dbReference type="eggNOG" id="COG0479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_2_0_11"/>
    <dbReference type="OrthoDB" id="1605514at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="250"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="37963" checksum="F3ACD7629DCD8E02" modified="2014-01-22" version="1">MERHFRVWRGDSSGGEIQDFDVEVNEGEVVLDIIHRLQATQTPDLACRWNCKAGKCGSCSMEINGKPRLGCMTRMSTFEEDETVTITPLRAFPIIRDLVTDVSFNYEKARETPAFAPPPGVKPGDYRMQQIDVERSQEFRKCIECFLCQNTCHVVRDHEENKEAFSGPRYFIRAAELDMHPLDDREDRKEYAQAHQGLGYCNITKCCTEVCPEHIKITDNAIIPMKERVVDRRYDPLVWLGRKIFRRDQMESPQPARAGGGSGGTTMAPSTPEGVTGVASGLSTSGARLPEPPAVNADGRLDVAELLSHQAGGPSPFGDDQEFPLPTGHIAYQHPEPDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA72</accession>
    <name>U5WA72_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uma2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562407.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39095"/>
    <dbReference type="EnsemblBacteria" id="AGZ46073">
        <property type="protein sequence ID" value="AGZ46073"/>
        <property type="gene ID" value="AFR_39095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7912"/>
    <dbReference type="eggNOG" id="COG4636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076312_4_0_11"/>
    <dbReference type="OrthoDB" id="2040599at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06260">
        <property type="entry name" value="DUF820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012296">
        <property type="entry name" value="Nuclease_put_TT1808"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008538">
        <property type="entry name" value="Uma2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05685">
        <property type="entry name" value="Uma2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uma2" evidence="1">
        <location>
            <begin position="19"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="193" mass="21903" checksum="0F2F24B02ADE5081" modified="2014-01-22" version="1">MTAVPEWMRPPRLEGWYADDLDHLPEAPRHTELIDGALVFMMSPQRSWHARLVTALVVSLTDQAPVGIEVEREMTIRLDDRNRPEPDLLVTTAPYAPDRTFYTPGEVLLVAEVVSPESAHRDRTVKLRKYAEAGIPNYWRVEDENGAPVVHTYELDGPTRSYAPTGIHRTKMVSPVPFEVELDLDRLIPGRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB31</accession>
    <name>U5WB31_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_34415</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561478.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34415"/>
    <dbReference type="EnsemblBacteria" id="AGZ45141">
        <property type="protein sequence ID" value="AGZ45141"/>
        <property type="gene ID" value="AFR_34415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6963"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_2_11"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="4">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="115" mass="12633" checksum="5F0A7A78882ADA8B" modified="2014-01-22" version="1">MPEDSLTTVTDDTFAELVLGSELPVVLDVWAEWCPPCGPVARTLAELAGEFTGKLVIAQLNIDENPVTGRNYRVMSMPTLLFFRRGVVVHTIVGARPKSQLRQALAGSFEAYVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB88</accession>
    <name>U5WB88_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_39990</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562585.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39990"/>
    <dbReference type="EnsemblBacteria" id="AGZ46252">
        <property type="protein sequence ID" value="AGZ46252"/>
        <property type="gene ID" value="AFR_39990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8091"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_4_11"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="4">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="13255" checksum="A269C2C3130FFA57" modified="2014-01-22" version="1">MATVALTTENFDEVTGKDGIVLVDFWASWCGPCVRFAPTYERSSEKHTEITFGKVDTEAQQDLAAKFKITSIPTIMAVRDGVIVFSQPGALPESALESLIDQVEKLDMEDVRKQLAAHNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBF6</accession>
    <name>U5WBF6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="4">dUTPase</shortName>
            <ecNumber evidence="4">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">dut</name>
        <name evidence="7" type="ORF">AFR_35295</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>dUTP + H2O = diphosphate + dUMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the dUTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35295"/>
    <dbReference type="EnsemblBacteria" id="AGZ45315">
        <property type="protein sequence ID" value="AGZ45315"/>
        <property type="gene ID" value="AFR_35295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7141"/>
    <dbReference type="eggNOG" id="COG0756">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068508_1_3_11"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00116">
        <property type="entry name" value="dUTPase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="PTHR11241"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase" evidence="6">
        <location>
            <begin position="29"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="17821" checksum="84B948B470111201" modified="2014-01-22" version="1">MPLADEEREEQIVTDTVAVQLRRLDPDLPLPAYAHPGDAGADLVAAEDVELVPGARQTVRTGIAIALPEGYVGLVHPRSGLAARLGVTVLNAPGTVDAGYRGEILVNLVNHDPAATAKISRGDRIAQLVVQRVERAAFHVVDELSDTARGQGGHGSTGGHVALPAQQQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBS7</accession>
    <name>U5WBS7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile)-lysidine synthase</fullName>
            <ecNumber evidence="6">6.3.4.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile)-2-lysyl-cytidine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile)-lysidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tilS</name>
        <name evidence="10" type="ORF">AFR_41550</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the tRNA(Ile)-lysidine synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41550"/>
    <dbReference type="EnsemblBacteria" id="AGZ46564">
        <property type="protein sequence ID" value="AGZ46564"/>
        <property type="gene ID" value="AFR_41550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8407"/>
    <dbReference type="eggNOG" id="COG0037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018869_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="PP-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015262">
        <property type="entry name" value="tRNA_Ile_lys_synt_subst-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="PTHR43033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09179">
        <property type="entry name" value="TilS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665903" description="tRNA(Ile)-lysidine synthase" evidence="7">
        <location>
            <begin position="26"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="ATP_bind_3" evidence="8">
        <location>
            <begin position="11"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="TilS" evidence="9">
        <location>
            <begin position="235"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ46564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="30983" checksum="65737FFE70D79D38" modified="2014-01-22" version="1">MRRALGPSGSVLVACSGGADSLALAAATAFVAPRLGIRAGLVTVDHGLQDGSAARAAAVAEWGRSVGLDPVEVATVDVGGRPGGPEAAARDARYEALSSAAERNTAGTVLLGHTRDDQAETVLLALARGGGPRGLSGMPFRKGVFVRPLLDVTRADTRKACAALGLAAWDDPHNADPAYTRARVRADALPALIAALGPAVVANLARTAALVAADVDYLDAAAAEALAAVRTPAGLPVPALAALPAAIRSRVLHAWAKELGAPGSALAHRHVVALDALVTAWHGQGPTSLPDGIVVGRREGHLVRVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC14</accession>
    <name>U5WC14_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_36200</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561832.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36200"/>
    <dbReference type="EnsemblBacteria" id="AGZ45496">
        <property type="protein sequence ID" value="AGZ45496"/>
        <property type="gene ID" value="AFR_36200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7325"/>
    <dbReference type="eggNOG" id="COG0575">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037294_0_1_11"/>
    <dbReference type="OrthoDB" id="1533756at2"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="387" mass="40167" checksum="7405CD0E8F8FB28A" modified="2014-01-22" version="1">MSYLEPRGGHTPSGGRASGQPDAPAPYVSGVPAEEPDPYATYRRQADAQQATESGPSWNAFTPGPAEAPAAPPVPEWSAPTAATSTSQAEWELTEAESPRGPGRRRAGRDGKSPAKPAGRAGRNLPAAIAVGLSMILVVLASLFVWKPAFLGVMAVAAGVGIWEMVRALKVTGAKAPMIPLLAGGVLMTGLAWYGSTDALELGLLVTVLAAGLWRLADGVEGLRRDLSAIALIAVYVPFLLSFGVLLARPEDGAKRVLITLIAVVFSDTGGYAAGVFFGKHPMAPTISPKKSWEGFAGSVTASAVGSSLLAYFLLDVPFYWGLLFGAVLSIVAVVGDLAESMLKRDIGIKDMSNLLPGHGGLMDRLDSILFAVPTAFLLLSLIAPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCF3</accession>
    <name>U5WCF3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562016.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37130"/>
    <dbReference type="EnsemblBacteria" id="AGZ45680">
        <property type="protein sequence ID" value="AGZ45680"/>
        <property type="gene ID" value="AFR_37130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7512"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_43_0_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28688" checksum="64AAA0439155CB6A" modified="2014-01-22" version="1">MKVMTDDGTEAWAEDEGRGTPILVIHGGMGDPSAWRPVTDLLHDRFRTVRLHRRQYRLDLPRPVTMAQEVSHVAAVAGELGRPLLVGHSSGAVLALEAMAADPDAYTGAVLYEPPTVIGEPLGGPKLVPAHAAIAAGKPGRALTIFLRDVVRLPAPMAWFAGAFVGRHPEYRRRVIRQLDDNDAIDALGVRLPAYADLDLPILLLGGDRSPRHLAERLDALEQALPRTRRLLMHGQGHNAERSAPARVAQAITKFLDEDIKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCI0</accession>
    <name>U5WCI0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative 3-isopropylmalate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36860"/>
    <dbReference type="EnsemblBacteria" id="AGZ45626">
        <property type="protein sequence ID" value="AGZ45626"/>
        <property type="gene ID" value="AFR_36860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7457"/>
    <dbReference type="eggNOG" id="COG0473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="2">
        <location>
            <begin position="3"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="36454" checksum="D32BAE0D981709DF" modified="2014-01-22" version="1">MARIAVVAGDGIGTEVTAEARKVIEAVLPDTEFDDYDLGARFYNRTKEVLPQAIEDELAAHDAILLGAIGDPSVPPGVLERGLLLKLRFDFDQYVNLRPSRLWPGTTSPLSGIKPGEIDMVVVREGTEGLYVGAGGVLHKNTPAEIATEESLNTRHGVERVIRDAFGRAQRRERRHLTLVHKTNVLTHAGGLWARTFAAVAAEFPDVTTEYQHIDAASMFLVSNPQRYDVVVTDNLFGDILTDIAAAVTGGIGMAASGSVNPERKYPSTFEPVHGSAPDIAGQGIADPAAAILSASLLLEHLGHTDEARRVSEAVAAEVASRTPGASLRTAEVGDRVAATAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCK5</accession>
    <name>U5WCK5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Release factor glutamine methyltransferase</fullName>
            <shortName evidence="6">RF MTase</shortName>
            <ecNumber evidence="6">2.1.1.297</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">N5-glutamine methyltransferase PrmC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein-(glutamine-N5) MTase PrmC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein-glutamine N-methyltransferase PrmC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prmC</name>
        <name evidence="9" type="ORF">AFR_37405</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = H(+) + N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37405"/>
    <dbReference type="EnsemblBacteria" id="AGZ45735">
        <property type="protein sequence ID" value="AGZ45735"/>
        <property type="gene ID" value="AFR_37405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7566"/>
    <dbReference type="eggNOG" id="COG2890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:protein-(glutamine-N5) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036009">
        <property type="term" value="F:protein-glutamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018364">
        <property type="term" value="P:peptidyl-glutamine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02126">
        <property type="entry name" value="RF_methyltr_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="Release_fac_Glu-N5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PrmC_N" evidence="8">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="MTS" evidence="7">
        <location>
            <begin position="104"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="179"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="272" mass="28670" checksum="01EB8E45D2F0BA9D" modified="2014-01-22" version="1">MLARATARLAEAGVASPRVDAELLAAHALGVGRGRLLLIDTLRGTELRRFDELVAERARRIPLQHLLGTAAFRHLELAVGDGVFVPRPETELLAGWGIEHTVPGATVVDLCSGTGAIALSVADEAEPARVLAVERSAAALAYLRRNAADFPAVQVVQGDVTDLGLLAECRGEVDVLLCNPPYVPDGTVVPAEVAEHDPAEAVFGGADGLVVIRPVIALAAALLRPGGVVGIEHDDVHASAVPDLLRSDGRFIDVTAHRDFSDRPRFATARRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCL5</accession>
    <name>U5WCL5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42290"/>
    <dbReference type="EnsemblBacteria" id="AGZ46712">
        <property type="protein sequence ID" value="AGZ46712"/>
        <property type="gene ID" value="AFR_42290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42290"/>
    <dbReference type="eggNOG" id="COG1106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_514722_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_15" evidence="1">
        <location>
            <begin position="3"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase_AAA_core" evidence="2">
        <location>
            <begin position="177"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="525" mass="57775" checksum="A920A7F4A60397BE" modified="2014-01-22" version="1">MIFGHVTVENYRSIEHSGDIKLGPITVLVGRNNSGKSSLIRAMYLLQQSAPFADSDIRHGSAVMHLVLQLVGKPRLNLASGEEVPHQYGDGKIALSMTNTGLSKFVAEDDDENTIGLSLASSAEPNNLIFPVLSGRRPHTLQQQVNRVNATTVGHQDSYVFSKIAELLANDLPESRRFRDICKRALGLNFGVINTSNNNQTLGVKITRYQEILIEAMGAGLAAALNLIASLSTATGQLFLIEEPENDLHPAALKVLLREIINSSDTNQFVISTHSNIVLSALGSIPDTSVVEVTSDDGAIPTSSYTAITDAESRLRVLRDLGYELSDFGLGQGWLIFEESTAELLIRNWFIPVFAPSLAILRTVAASGVSRIRPMFRDIQEMFLFAHLEKIYKGRVWVIADGDEAGREATDQLKKSYKTWDPSHFANWDKDNFEFYYPSDFAEDVSRVLAIGDGVKKKAAKGKLVEKVWAWIRADEARARTAFESSAAPVIDTLRAIEKQMHGLGSAWNLSEAVEKDSEQSQAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCQ0</accession>
    <name>U5WCQ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562115.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37630"/>
    <dbReference type="EnsemblBacteria" id="AGZ45780">
        <property type="protein sequence ID" value="AGZ45780"/>
        <property type="gene ID" value="AFR_37630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7611"/>
    <dbReference type="eggNOG" id="ENOG50330EJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169726_0_0_11"/>
    <dbReference type="OrthoDB" id="2071915at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="9892" checksum="A5DD37B2665B50DF" modified="2014-01-22" version="1">MPSQLLRITALVELGSLILLLTNLATVHWPGVASLLGPVHGCAYLLVIGSTLREARSLRTRLCAFIPGFGGLLVVRRLSTGGPGKDVSMSPTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD54</accession>
    <name>U5WD54_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Abasic site processing protein</fullName>
            <ecNumber evidence="7">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_38135</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562216.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38135"/>
    <dbReference type="EnsemblBacteria" id="AGZ45881">
        <property type="protein sequence ID" value="AGZ45881"/>
        <property type="gene ID" value="AFR_38135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7715"/>
    <dbReference type="eggNOG" id="COG2135">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035990_6_2_11"/>
    <dbReference type="OrthoDB" id="1718602at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:cellular response to DNA damage stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018142">
        <property type="term" value="P:protein-DNA covalent cross-linking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="PTHR13604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="SSF143081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="229" mass="24870" checksum="9C561F24C55982CA" modified="2014-01-22" version="1">MCGRYATTRSEADLSLLFDAVTVAEALGPSWNVAPTDPVPVVRQSQRHDARVVDTARWGLLPPWAKDPRAGSRMINARAETVATSPAFAPSFQRRRCLVPADGWFEWVRDGGKRQAYYMTPADSSVLAFAGIWTMWREETLTCSVLTTAALGDLAAVHDRMPLILPRERWSAWLAGGGDAPALLEPLSPAELAAIEVRPVRPDVGNVRNNGPELVTAAEPAVAPEATLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD65</accession>
    <name>U5WD65_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38555"/>
    <dbReference type="EnsemblBacteria" id="AGZ45965">
        <property type="protein sequence ID" value="AGZ45965"/>
        <property type="gene ID" value="AFR_38555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7801"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_13_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="15267" checksum="CC667087B3123463" modified="2014-01-22" version="1">MDELSLLRRARDRIDREYAQPLDVADLARTAHLSAGHFTRRFRAVFGETPYGYLLTRRIERAKTLLRRGDLSVTEVCVQVGATSLGSFSASFSALVGESPSAYRARDHRAAAVIPGCVTRRLGQPVRFREAGRGRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDD8</accession>
    <name>U5WDD8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chromosome replication initiation inhibitor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38535</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562295.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38535"/>
    <dbReference type="EnsemblBacteria" id="AGZ45961">
        <property type="protein sequence ID" value="AGZ45961"/>
        <property type="gene ID" value="AFR_38535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7797"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063829_0_1_11"/>
    <dbReference type="OrthoDB" id="951478at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017685">
        <property type="entry name" value="ArgP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03298">
        <property type="entry name" value="argP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="30788" checksum="85CE7176EB5CF5E0" modified="2014-01-22" version="1">MDQVQLVTFQAVIEEGSFDAAARALHVTPSAVSQRIKALEQDVGQVLVRRVKPCEPTEAGQSLVRLAGQIALLEREALDAARGAPAGGGRTRLAVVVNADSLHTWFLRALAAVPSDLALSFDLHQDDQDHTADLLRAGTAMAAVTAQDVAVQGCRVEKLGAMRYLTVAAPGTFEDYATSPMIVLNRKDRLQHRFLAAQTRRRLDPPIHYVPAAAAFVDAIRLGLGWGLVPEQIARDDITAGRFVDLAPGRHLDVPLHWQYWRLQSAAMTALTEAVKAVAAETLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDU9</accession>
    <name>U5WDU9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39160"/>
    <dbReference type="EnsemblBacteria" id="AGZ46086">
        <property type="protein sequence ID" value="AGZ46086"/>
        <property type="gene ID" value="AFR_39160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39160"/>
    <dbReference type="eggNOG" id="ENOG5033Y0X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1667565_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="18256" checksum="F1E22732DD2CA079" modified="2014-01-22" version="1">MLMRYVVISRAEGNWPGNNLDPEPYLRVLPELLPQLPPGAREFAGAADHYDFTAVRCVKDLQFGSLTAGEAGAAQISMSLRFAPNPAKHDAALTVRYLGVSEFRIDVKQLGEKARVWPESRRLGDLQLDEILPVDGGCTHEIQLTGGHIFVRCTDLVAEWETTAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE30</accession>
    <name>U5WE30_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-oxoglutarate ferredoxin oxidoreductase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562612.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40130"/>
    <dbReference type="EnsemblBacteria" id="AGZ46280">
        <property type="protein sequence ID" value="AGZ46280"/>
        <property type="gene ID" value="AFR_40130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8121"/>
    <dbReference type="eggNOG" id="COG1013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_1_0_11"/>
    <dbReference type="OrthoDB" id="981516at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="59"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="348" mass="37460" checksum="F522178DD4FBBCE7" modified="2014-01-22" version="1">MVSSTIPLTPVKLTAKDFKSDQEVRWCPGCGDYAILAAVQQFMPELGIARENTVFVSGIGCSSRFPYYMNTYGMHSIHGRAPAIATGLSASRPDLSVWVVTGDGDALSIGGNHLIHALRRNVNMKILLFNNRIYGLTKGQYSPTSELGKITKSTPAGSADSPFNPLSLALGAEATFVGRTIDSDRKHLQSVLRAAAEHEGSAFVEIYQNCNIFNDGAFEVLKDPGTRDDHLIRLEQGQPITFGAEAQYSVVHPSGGFGLSVRDSAELGGETPVIHDATVDDPAYAFALSRLSGSDLGTTPIGVFRNVQRPSYDELIQKQLEDAKAQVTGTPDQMLGELLNAGDTWTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEA4</accession>
    <name>U5WEA4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40035"/>
    <dbReference type="EnsemblBacteria" id="AGZ46261">
        <property type="protein sequence ID" value="AGZ46261"/>
        <property type="gene ID" value="AFR_40035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8102"/>
    <dbReference type="eggNOG" id="ENOG50331GF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128226_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="17295" checksum="030C7BDF2F8122B0" modified="2014-01-22" version="1">MTTELVAEAIKKAGIAWIGAAGGGPARGLWVMPLDGALIVVCGLGEQAAPGLAEATRAEVRLRGDNGGLILIVEAQVERLTPGSEAWAEIAPQLAGKRLNASGTAEQVVARWAETGCAVLRLTPTEDLVAAPGLPSDSAAAPPRETPARIRVRSPFKLHRARKRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WED9</accession>
    <name>U5WED9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Usp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40755"/>
    <dbReference type="EnsemblBacteria" id="AGZ46405">
        <property type="protein sequence ID" value="AGZ46405"/>
        <property type="gene ID" value="AFR_40755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8250"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_10_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="15"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16076" checksum="E23D18156CEA9B3D" modified="2014-01-22" version="1">MVSRFELGTDGPGAIIVGVDGSETSLRAAAYAAGLARRQHTRVIVVYARSASSLLTLADHTGLATRAAVETHDAIEEQLRTELAKQARIWGVDASLEVRRGDPLTVLTEVARDVRADAVIVGSSQSRGHRIAGSLAVRLVRCGRWPVTVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEY5</accession>
    <name>U5WEY5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562843.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46511">
        <property type="protein sequence ID" value="AGZ46511"/>
        <property type="gene ID" value="AFR_41285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8355"/>
    <dbReference type="eggNOG" id="ENOG502ZE2I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080235_0_0_11"/>
    <dbReference type="OrthoDB" id="1660095at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="257" mass="26562" checksum="E0A2FFA4128099A9" modified="2014-01-22" version="1">MGSLSDNGGNWPPDGGSPDGLPDFPEEWGVIVIPDDLSELSEEVTAVREELRQTQEKTRWERFADRPGMRSLRRIGAASLRAPVLIISMAVLVTVASLFASAWPGPSRSPATQRTANTTDDRGDRLPALELIGTDGKTVPIRGQLPAVVLLTDGCDCAQLIADTAAAVKPGITVVTVVAGAAPGQTGAAPATGAVPQAPGKVVSALRDPTGELRSGLNLGAPDGTAAALLVDSSGEIVRAVPRTASIEDLRPDLARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF62</accession>
    <name>U5WF62_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase</fullName>
            <ecNumber evidence="2">2.7.6.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_41460</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562878.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41460"/>
    <dbReference type="EnsemblBacteria" id="AGZ46546">
        <property type="protein sequence ID" value="AGZ46546"/>
        <property type="gene ID" value="AFR_41460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8389"/>
    <dbReference type="eggNOG" id="COG0801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097916_0_0_11"/>
    <dbReference type="OrthoDB" id="1953248at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="SSF55083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPPK" evidence="8">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="175" mass="19327" checksum="66FC557086B136EB" modified="2014-01-22" version="1">MTRAILSLGSNLGDRYAYLKEAVTQLGDSAVLMSGVYETPPWGDADQPAYLNAVVLAVDPAADPRSWLDRVRELEKAAGRARDPERRFGPRTLDVDVIAVWRDDDTPVLSDDEELTLPHPRAHLRAFVLKPWLDLQPYAQLPGHGWVTDLLNAPELTADANALTPRPDLPLESLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFT5</accession>
    <name>U5WFT5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DoxX family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_42710</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42710"/>
    <dbReference type="EnsemblBacteria" id="AGZ46796">
        <property type="protein sequence ID" value="AGZ46796"/>
        <property type="gene ID" value="AFR_42710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8644"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058421_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="18302" checksum="D595E8B4CF9EA827" modified="2014-01-22" version="1">MLGAIFVVSGVRIVLDPDAKVDAAKRVTDKVGPMLQRVDPRLPSDARTLVQAKAASDVVAGLMLASGRMTRPAALVLAANLVPTTFAGHPFWTMPKPERTQHETHFLKNLALLGGLLLAAADTEGRPGLSYRTSHAVERSQRSVRRAVRTAKREARIARRSAVAARKIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNA6</accession>
    <name>U5VNA6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2786 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357396.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00820"/>
    <dbReference type="EnsemblBacteria" id="AGZ38453">
        <property type="protein sequence ID" value="AGZ38453"/>
        <property type="gene ID" value="AFR_00820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.164"/>
    <dbReference type="eggNOG" id="ENOG5031B71">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098224_0_0_11"/>
    <dbReference type="OrthoDB" id="1620747at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024498">
        <property type="entry name" value="DUF2786"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10979">
        <property type="entry name" value="DUF2786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2786" evidence="1">
        <location>
            <begin position="6"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="23945" checksum="9BDE64FAEF3A5635" modified="2014-01-22" version="1">MSDDPLLARVRKLLAKAEDPACTPAEAEAFTAKAAQLIAKYGVDQALLAGADPALDPVGDRVVTVVPPYARDKAGLLGAVAVAMRCRVVHLERRGAARIHLFGHAADLERVELLFTSLLVQAAHGLAATAVPSFDHPAAFRRSWLVGFTEAIRNRLTEAEREAAADVPGAALVLVDRTELVDRKRDETYPKLMRLGPRRLAGTGRRLGYAAGRTADLGGTGLPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNS6</accession>
    <name>U5VNS6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357395.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00815"/>
    <dbReference type="EnsemblBacteria" id="AGZ38452">
        <property type="protein sequence ID" value="AGZ38452"/>
        <property type="gene ID" value="AFR_00815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.163"/>
    <dbReference type="eggNOG" id="ENOG5031M1Y">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131923_0_0_11"/>
    <dbReference type="OrthoDB" id="1654344at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="13479" checksum="96E36720302CDE82" modified="2014-01-22" version="1">MTDIPQHALDDAAQVLHCALSGDSDAVVGTFDAVVDRSGVVGAYDVAWCLAATMVGQNVPRGAWTLDFPGIDDARYDKRWVARFVSAYVNGDISTGEALFGAAIADGQLPDCLLALAGSTVATLRHRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP57</accession>
    <name>U5VP57_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NlpC/P60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_01465</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357525.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01465"/>
    <dbReference type="EnsemblBacteria" id="AGZ38582">
        <property type="protein sequence ID" value="AGZ38582"/>
        <property type="gene ID" value="AFR_01465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.294"/>
    <dbReference type="eggNOG" id="COG0791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034352_0_0_11"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665912" description="NlpC/P60 domain-containing protein" evidence="3">
        <location>
            <begin position="44"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="NlpC/P60" evidence="4">
        <location>
            <begin position="278"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="408"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="420"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="498"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="548" mass="56511" checksum="1CEC1593094B6C70" modified="2014-01-22" version="1">MATKRQYGRDRSLRVRPWWRPLAYSAATAAAIALLFNPLPASAAPIAPAAPPAVPDAGSRPQPIGTIAMPGQAGVPTAAPATTTPILATGVAMSPVLAKIEKGRLELTTLGEALTKVKEDVDLTNQQLVIANQKVTTTAAAVAQAQTEVSDAAASAVRDAAALPPGGIGSGLSDLDSLARINRGDAATEQAASRQLTIAQTAYTGALAEQQSLTTHLTQVTADRDKRQKDLDKKTAAQQKLERDNAPAISADDAANAAQDAAVGQGILAGENAGRGADARALAAVRFALAQRGDPYVWSEEGPDEYDCSGLMYAAYRSDPAGNFPLTRVSRDQYYQTRGKAVDRYSLLPGDLLFFSSSSSWKGIHHVAMYAGDGMMVEAPRTGLNVRLTPVRWTRLFGATRIYGSVEGTVEGPALGSPDPETPSDNPPATKPTTKPTRPTTKPPTTKPTTKPTTPTTKPTTKPTTPTTKPTTPTTKPTDPTTPPTTPATPPTTPAAPTTQPTGATPTGSDDDAESSPSDSGSSSTKSASASAAAASKAAETSASASGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPG9</accession>
    <name>U5VPG9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_02050</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02050"/>
    <dbReference type="EnsemblBacteria" id="AGZ38699">
        <property type="protein sequence ID" value="AGZ38699"/>
        <property type="gene ID" value="AFR_02050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.415"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_7_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="15509" checksum="265AA3F20CFD3760" modified="2014-01-22" version="1">MGARGVILDDQNRLLLIQRSDNRRWAIPAGAMELGESMEECAIREVWEETGLRATSLTPFAFYSAYTYTNEWGHTYQQILMSFRIHTWEGELQKVTDESVDAGFFPLDALPGPKSMIIDETLADLASFEATGRMVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPK4</accession>
    <name>U5VPK4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357677.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02225"/>
    <dbReference type="EnsemblBacteria" id="AGZ38734">
        <property type="protein sequence ID" value="AGZ38734"/>
        <property type="gene ID" value="AFR_02225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.450"/>
    <dbReference type="eggNOG" id="ENOG5032SQA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150044_0_0_11"/>
    <dbReference type="OrthoDB" id="1893426at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014447">
        <property type="entry name" value="UCP006909"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006909">
        <property type="entry name" value="UCP006909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="98" mass="11245" checksum="218CDD13AD6A6049" modified="2014-01-22" version="1">MIFKAVRDGAPYPDHHTTLKRWAEIPPRPIRLADLITTKRELALDKLLAEDSTFYGDLFPHVVEWHGGLYLEDGLHRALRAALQQRNQIHARVLVIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPV7</accession>
    <name>U5VPV7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH luxR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03520"/>
    <dbReference type="EnsemblBacteria" id="AGZ38993">
        <property type="protein sequence ID" value="AGZ38993"/>
        <property type="gene ID" value="AFR_03520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.707"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_625040_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="373"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="438" mass="46882" checksum="91EF518FFC47CD36" modified="2014-01-22" version="1">MALSRTTADPRVLFEAFLLLSRTATAAELRPLVQDMELMRWSAPAPETDFLFHVLAGVVGARARDPASFARLETALVLFDEHGLAGDPLFLECALLATLTLIRPHETRARFAAHVDRLDLDPGRRARLVAMIGLGDAWAGNLVRGQAEMLEARDLAARAGRLEIQAEVTSWLIKCEALRGDLAASAAHLAEARELAGRTGSAWVAGHIIEGSAALQFALGDTEAWVGLLELMVGAEQGVDSGLLFEYRWELATHHALRGESGTAAALLDGVPEAPVGLPGGPAMAAWRDWISAPDDAGLIAAFEGALKDLVRPDERLPRARMCWLLGVAHGRAGRRAPAVRLLEAACSGYATIGAAGMLALAAADLRAVTGSPPKDPWPLTGAEVRVAVAVADGLSNKEAAERLFVSVKTVEFHLGNIFRKLDVRNRTELARRRDRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ55</accession>
    <name>U5VQ55_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03275"/>
    <dbReference type="EnsemblBacteria" id="AGZ38944">
        <property type="protein sequence ID" value="AGZ38944"/>
        <property type="gene ID" value="AFR_03275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.659"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3347">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024866_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013454">
        <property type="entry name" value="Bifunc_RhaD/ADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02632">
        <property type="entry name" value="RhaD_aldol-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="2">
        <location>
            <begin position="8"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="676" mass="70801" checksum="70083D5640DDB9D8" modified="2014-01-22" version="1">MSNPAVSDLLARSNRLGADPRNTNYAGGNTSAKGAETDPVTGTPAELLWVKGSGGDLGTLTEPGLAVLRLDRLRALAGVFPGVDREDEMVAAFDYCLHGRGGAAPSIDTAMHGLVDVAHVDHLHPDAGIALATAADGPALTKEIFGDRVLWVPWRRPGFQLGLDIAAVQRANPQAIGVILGGHGITAWGATSAECETNSLEIINTAAAYLAAHGREAPFGPLIHEPLPDPVRRERAAALFPVVRGLASTDRAQVGHYTDSEVVLDFVRRERMPELAALGTSCPDHFLRTKVKPLVLDTAPDAPLEEVEARLKELHAAYRDDYRGYYERHAGPDSPAMRGADPAIVLVPGVGMFSFGANKQTARVAGEFYVNAINVMHGAESVSTYAPIDEAEKFRIEYWALEEAKLQRMPKPKPLATRVAFVTGGGSGIGKAIALRLAAEGACVVVADRDFEAATSVAGEIGSADVAVAVAADVTSEAEVTAALREAVLAFGGVDLVVNNAGLSISKPLLETTEADWDLQHDVMAKGSFLVAKAAAAVMIAQGLGGDIVYISSKNSLFAGPNNVAYGAAKADQAHQVRLLAAELGGHGIRVNGINPDGVVRGSGIFAGGWGAKRAAVYGVPESELGAYYAQRTLLKREVLPEHVANAVFVLTGGELSHTTGLHIPVDAGVAAAFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQK4</accession>
    <name>U5VQK4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ring-cleavage dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358007.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03865"/>
    <dbReference type="EnsemblBacteria" id="AGZ39062">
        <property type="protein sequence ID" value="AGZ39062"/>
        <property type="gene ID" value="AFR_03865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.781"/>
    <dbReference type="eggNOG" id="COG3384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078149_1_1_11"/>
    <dbReference type="OrthoDB" id="1728007at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LigB" evidence="1">
        <location>
            <begin position="8"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="32200" checksum="571E612CB6FF6EA1" modified="2014-01-22" version="1">MATVVAVIASTHHPFYYRASTATGEDRPPFADEWVRKITAFRETLTAARPDVLVMVGSDHFHQLWLDNMPQFLVGKAPFYDANWYNEEREFGLPKMLLKGNEDLSAHILRAGLDEGFDLAFSNELRIDHSITCPIITLRPEADLPIVPIYTNIFAPPLPQPKRFVQLGQTIRQVIESWPSHLRVAVIGTGHLSLELGGPRQFGPHGPDPEFDQKAVEWIATGDLERCLHEVTLDSLHSPGNATHGFMDFMLMMGVAGAGRKADYVDSLDLFHTMEAYFTWYPNGVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQW8</accession>
    <name>U5VQW8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05395</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05395"/>
    <dbReference type="EnsemblBacteria" id="AGZ39368">
        <property type="protein sequence ID" value="AGZ39368"/>
        <property type="gene ID" value="AFR_05395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1092"/>
    <dbReference type="eggNOG" id="COG1252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021377_7_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006116">
        <property type="term" value="P:NADH oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="PTHR43706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="2">
        <location>
            <begin position="5"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="438" mass="48018" checksum="5B11EEF03E6A7D33" modified="2014-01-22" version="1">MGLYAALRLSKKLNSRQAEVIVIDPQPHMTYQPFLPEAAAGNISPRHSVVPLRRELKRCRIVSGEVTRIEHARKTVTVQPIEGPVKEIAYDHIVVAPGSVSRTLPIPGLRENGIGFKTIGEAIYLRNHILDRLDIAAITPDPEVRKASLTFVFVGGGYAGIEALAEMEDVVRDALKYYPELKKDEVKFVLVEATNRILPEVGPDMGAYAARQLDQRGIDLRLETRLESCVDGQIKLSDGDSFPAETLVWTAGVKPSPMLDHTDLPRGPRGHLTCIPTLQIVDGDRVLDGAWSAGDCAQVPDLANPGAWCSPSAQHAVRQAAQLADNIRAVVIGATPKDYKHKYAGSVASLGLYKGVAQVYGIKLKGLPAWLMHRTYHMSRIPSFNRKVRVLADWTLAFILKREVISLGQLHAPREEFTHVTPPLSDDRAAESVGAGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRF3</accession>
    <name>U5VRF3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357249.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00080"/>
    <dbReference type="EnsemblBacteria" id="AGZ38305">
        <property type="protein sequence ID" value="AGZ38305"/>
        <property type="gene ID" value="AFR_00080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.17"/>
    <dbReference type="HOGENOM" id="CLU_118434_0_0_11"/>
    <dbReference type="OrthoDB" id="2179419at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665608" evidence="2">
        <location>
            <begin position="26"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="14379" checksum="C5698D31EC5791ED" modified="2014-01-22" version="1">MEERRSARVGTVSGLALLAGGTAVAAVAGGTHSIFATILLGMLAVGMGHALLTEIRRQARRRSLGGWAAEDTANTVLLASWAAGALIVTTLAVAPTMVRAVGVMLTFGYAVSCIYFVRERRRTISTPAPDPATVPAETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRH8</accession>
    <name>U5VRH8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359000.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39469">
        <property type="protein sequence ID" value="AGZ39469"/>
        <property type="gene ID" value="AFR_05900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1198"/>
    <dbReference type="eggNOG" id="ENOG503342X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083559_1_0_11"/>
    <dbReference type="OrthoDB" id="1721189at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="191" mass="21819" checksum="0CDB8C86A174E1EE" modified="2014-01-22" version="1">MTTSAGSRTRFKASVFLSYPKPFMQRQQAFIERICGYLDDRGFSPRTLGVTDYDLDAPLKAVRRLMLESNGLITVAFRRTYIEKGTGNHRTDLSDRQSYEVDGRWTTSPWSQIEPAMAYQLGLPILILREEGVIPEGMLQRGVAGMYMPEFDLGTSLDDYFGSPEWNDVIFKWEAQVRTVVDRKGSPPKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRT5</accession>
    <name>U5VRT5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative phosphohistidine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357320.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00435"/>
    <dbReference type="EnsemblBacteria" id="AGZ38376">
        <property type="protein sequence ID" value="AGZ38376"/>
        <property type="gene ID" value="AFR_00435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.89"/>
    <dbReference type="eggNOG" id="COG2062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084603_2_1_11"/>
    <dbReference type="OrthoDB" id="1707924at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="18444" checksum="353D0360B5E62A73" modified="2014-01-22" version="1">MTVRTLVLLRHAKAETPGELPDFDRHLTAAGRSAADAAGSWLADERLRPGLVICSTAARTRETWQGVSVAMAQADPDAHSPEVHYEQSLYDGGRTEVIDLLRAVPDTVHTVLVIGHNPTMSDVSILLRPWEENAVLESLKTSGLAVHRAEGSWSDTEPGSLPLVLAHTARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS06</accession>
    <name>U5VS06_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359098.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06390"/>
    <dbReference type="EnsemblBacteria" id="AGZ39567">
        <property type="protein sequence ID" value="AGZ39567"/>
        <property type="gene ID" value="AFR_06390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1296"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100916_0_0_11"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="20671" checksum="7285C0B83F3140E2" modified="2014-01-22" version="1">MKTSGDRWADLALHPVRIRILRALAGARLTTHDLVPLLPDIPHATLYRHLSTLVKGDLIQVVEERRIRGAVERVYALATPGAPMDPAALATATPDDHARYFTAFMSSLLSEFSRYLARDRVDFAADGAGYQQLMLHLSDEELARFAADLGQVIGPLLANKPGEGRVPRLLATILLPADQPATTGEERSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS82</accession>
    <name>U5VS82_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ANTAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_07125</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07125"/>
    <dbReference type="EnsemblBacteria" id="AGZ39714">
        <property type="protein sequence ID" value="AGZ39714"/>
        <property type="gene ID" value="AFR_07125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1454"/>
    <dbReference type="eggNOG" id="COG3707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_766446_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ANTAR" evidence="4">
        <location>
            <begin position="291"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="14"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="361" mass="37410" checksum="A1F0D8B29F9345AA" modified="2014-01-22" version="1">MDMTFVFTDAHGNIGQMSAPQRSSGRLVSRRLPPSTGLPGNPLTAGPARVSGLGRSRGPLAAPTLPSAEITSGELAILAATPEARRTAGSRKSRRGAERAKATGNDRAARPVRRSGPVTDTGGRVRGVPASTVLPAGPHPVELAGLLHELSVRLLGADDMPQALDRLAAFAASAVPGALRCSVTLIGEGGPPVVAASGPRAQALDDLQYATGQGPALEAARNRVLVTAQELQNDPRWPELAVCARVEGVHGIVSIPLDVQRTSVGALSLFVAEPGGVDPELLLTTMALVNQAEVLLGELRRRESLAEGATVDRAAGVIIAQRGCGVQEAYDVLRETSQRLGIDRRAVAERLIAAAARNAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSB1</accession>
    <name>U5VSB1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">GTP pyrophosphokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07040"/>
    <dbReference type="EnsemblBacteria" id="AGZ39697">
        <property type="protein sequence ID" value="AGZ39697"/>
        <property type="gene ID" value="AFR_07040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1436"/>
    <dbReference type="eggNOG" id="COG0317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1159163_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="26088" checksum="CDA41CB3AF9AB8F5" modified="2014-01-22" version="1">MPLHAITEVYGEAGLRDRFALELLELPADDRAQLEEALELAARLHADDRRVREPYLNHLLRVAIRIMRYYGIRDVDVLTAALLHDAVEDHPAELAGLPGSLGHQVLTDAAVAELARRFNPRMAELVRSVTNPEYDPERDKHEQYRAHVADSLDRDPWARVIKVSDFTDNGVGVIHTTYDKARSSAIKYRPLVPKLRELVGRPDTPLSLAAKDHILDQLDLAEERFAAILDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSJ7</accession>
    <name>U5VSJ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_01980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357628.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01980"/>
    <dbReference type="EnsemblBacteria" id="AGZ38685">
        <property type="protein sequence ID" value="AGZ38685"/>
        <property type="gene ID" value="AFR_01980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.401"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_11"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="214"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="278"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="529" mass="54428" checksum="69345037FC344EF5" modified="2014-01-22" version="1">MTRSPADLSVRIKIQAAVGFAALVALAVGLIGLVALSSVNSSAQHLYRHNVAGVTAVGQIDAALSSARLDTANHLISDTTATEAKNASDFNADANAFKTALAAYQETDPAGDRSVITDLEAKWLAYEKIALGKLLPAGARNDVEAWQTIRDAEVSPLMATATGHIEALIAAETADAAHTAALVESKYQDSRTQSIVLLVAGLTGALFIGFFVSRGILRSLGRVEAVCHGLAAGDLTRSTGLTSRDEPGRMGRALDAALGTLRGTVSTIDSSASSLAGASEQMSGVAQQIAASAEQTSAQAATVSSAAEEVSRSVDSVSAGSEEMGAAIREISQNATEAARVAGEAVELTAATSRTMNKLGESSAEIGTVIKVITAIAEQTNLLALNATIEAARAGEMGKGFAVVASEVKDLAQETARATEDISRRVEAIQSDTGGAVTAIEEITRVIAQISDFQTTIASAVEEQTATTAEMNRSVGEAAAGTGDIAQNITGVAEGARLTSEGVTQSQQATRELARMSTELTTLVSNFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSP2</accession>
    <name>U5VSP2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5'-nucleotidase</fullName>
            <ecNumber evidence="3">3.1.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08595</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359539.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08595"/>
    <dbReference type="EnsemblBacteria" id="AGZ40008">
        <property type="protein sequence ID" value="AGZ40008"/>
        <property type="gene ID" value="AFR_08595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1749"/>
    <dbReference type="eggNOG" id="COG0496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_1_1_11"/>
    <dbReference type="OrthoDB" id="1909278at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SurE" evidence="6">
        <location>
            <begin position="5"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="28543" checksum="E1F292E146B1DA09" modified="2014-01-22" version="1">MTPRILITNDDGIDAPGIRHLTRAAARQGLDVVVAAPLTEASGTSAAMTAVEKNGKIVVQQRELAGARNVPAYGVAASPAFIVLLALREAFGPPPTIVLSGINRGANAGAAVVHSGTVGATLTASHAGLHGLAVSLDVLSPANGTASSGGQAIADLDKADDEKRHWDSAAGLAMKLLPTLTHTPPGTVLNLNVPDLHVDGIRGLRRGSLAKFGQVQMSIAEAGEGFVRTAVQAADEALEPGTDLAYLAEGFAVVTPIRSPGEATDVQFNIESIVVQTPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSQ6</accession>
    <name>U5VSQ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl hydrolase, family 76</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357693.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02305"/>
    <dbReference type="EnsemblBacteria" id="AGZ38750">
        <property type="protein sequence ID" value="AGZ38750"/>
        <property type="gene ID" value="AFR_02305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.466"/>
    <dbReference type="eggNOG" id="COG4833">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028686_2_0_11"/>
    <dbReference type="OrthoDB" id="504223at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666028" evidence="1">
        <location>
            <begin position="25"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="477" mass="52066" checksum="3BA678F0586DC929" modified="2014-01-22" version="1">MRHKWIAALTVAATVLLQVQTSSAAQAATICNRYCDGRDPALSPGDRVATTSTLHGRSFKVHINDTDAMIWATVENGQAGDQVWLDRSFDGGRTWSGDSRLGDTTVPAGAGGWRTSMFNVDNWGSRGVGVLRACGKASDRPELTCTGWARSNWNAADRRTAAATALMMRFDQGTGLFDTNGWWTGANALTALIDNSRITGMHSYDYAIATTYDKQINARQGQFRNEYLDDTGWWGLAWVAAYDRTGDSRYLSTARADADHMFSYWDTRCGGGIYWRTDRTVKNAIANSLYIQLNAALSQRIGGDSVYRGRAQAGWSWFQSTGMINSSNLVNDGVNLSTCRNNGDVTWTYNQGVLINALVQLNRLTGDANALTVARRIGDAATTSTYLNNNGILREPNEPDQCSGDGVSFKGPFVRGLGVLNAAVGRPYDAYLRRQADSAYAKNRTSFDAYGSHWAGPYVSTNHSCQHSVLDLLNAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT37</accession>
    <name>U5VT37_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357833.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38890">
        <property type="protein sequence ID" value="AGZ38890"/>
        <property type="gene ID" value="AFR_03005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.604"/>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078980_0_0_11"/>
    <dbReference type="OrthoDB" id="210346at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666036" evidence="1">
        <location>
            <begin position="26"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="27074" checksum="40FB0DF58C1AF130" modified="2014-01-22" version="1">MRSRSLLASAVLAGAFLGSVPGVAAAHQQAGAAPVTVSAAARTAVLHATAARAAGGTGRLVARYAFDGARSGSIPDDSGNGHTLRLITGNGGTVSPVQHGSGQALRFPGKCSAKKCPHAALQSPSAASLNPGTRPISYGAMVLLTRGQTSKGQNVVQKGYSATSSQYKLQIDGAAGRASCVLVGAKPGIKLVTSSRSVADGAWHTIECRRQGTNLTVLVDGAVRGNRAISAQLSVSNNRPFSVGGKGAYSDNDQFQGTLDNVWVRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT99</accession>
    <name>U5VT99_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_03355</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357903.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03355"/>
    <dbReference type="EnsemblBacteria" id="AGZ38960">
        <property type="protein sequence ID" value="AGZ38960"/>
        <property type="gene ID" value="AFR_03355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.675"/>
    <dbReference type="eggNOG" id="COG4941">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035311_1_0_11"/>
    <dbReference type="OrthoDB" id="1469807at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="107"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="393" mass="42199" checksum="0BF1FD0D500771A6" modified="2014-01-22" version="1">MTDTARAVADAGVESRPRIVAALIRITGDWTLAEDCAQEALALALERWPKDGVPANPGGWLMTVARNRAVDVLRRATVERRKLEELAMLASTEPEEVPAVDDRLRLIFTCCHPALALEARVALTLRTIAGVPAADIARTFLVTESTMTRRLTRAKAKIATAGIPYRVPTGAALTERLPGVLAVLYLLFTRGYDTEGEPAFAEEAIRLARLLRALMPGEPEVSGLLALFLLQHSRRAARRDADGNLLTLDQQDRGRWDHAAIGEAVALLPAQPGPYALQARIAACHVASPSDWPTAATLYDELYGLLPTPVIALNRAVAHGYAHGAASGLVLLAEARAGGALDAYPYVLAVEADLTARAGDVERAVSLFERAAAAAPTEPERRALLTRAEDLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTA9</accession>
    <name>U5VTA9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chitinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_09645</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359881.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09645"/>
    <dbReference type="EnsemblBacteria" id="AGZ40218">
        <property type="protein sequence ID" value="AGZ40218"/>
        <property type="gene ID" value="AFR_09645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1966"/>
    <dbReference type="eggNOG" id="COG3325">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3979">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002833_14_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003610">
        <property type="entry name" value="CBM_fam5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036573">
        <property type="entry name" value="CBM_sf_5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00495">
        <property type="entry name" value="ChtBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51055">
        <property type="entry name" value="SSF51055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="SSF54556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665730" evidence="6">
        <location>
            <begin position="32"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="7">
        <location>
            <begin position="121"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="78"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="490" mass="51511" checksum="8DD520ADC66FEA37" modified="2014-01-22" version="1">MNKTLRRALWAGAFVAITTAAVPMVQASAAAACAPAWSSSAVYVKDNQVSYQSKNYTAKWWTQNEVPTASGEWGVWSDKGACGGGTTPPTTPPPTTPPTTPPTTPPTTPPTTPPTNGPAGKAVVGYFAEWGVYGRNYHVKNLVTSGSASKLTHILYAFGNTTGGQCSIGDAYADYDKAYTAADSVDGVADTWDAGALRGSFNQLRKLKKAYPNIKVIWSFGGWTWSGGFTQAAANPTAFANSCYNLVKDSRWSDVFDGIDVDWEYPNACGLSCDASGPNSYNNVITALRNKFGSSFLVTSAISADGSNGGKLDVADYASGISKLNYVFPMTYDYFGAFAPTGPTAPHSPLTSYSGIPQQGFWSDAAIQKLKSKGVPSNKMLLGIGFYGRGWTGVSQTAPGGSATGAAPGTYEAGIEDYKVLKNSCPSSGTVGGTSYSKCGSNWWSFDTPSTITSKMAYAKQQGLGGAFFWEFSGDTSNGELITAIKNGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTD8</accession>
    <name>U5VTD8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Regulatory protein afsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_09100</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AfsR/DnrI/RedD regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359664.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09100"/>
    <dbReference type="EnsemblBacteria" id="AGZ40109">
        <property type="protein sequence ID" value="AGZ40109"/>
        <property type="gene ID" value="AFR_09100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1851"/>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004665_1_3_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15831">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005158">
        <property type="entry name" value="BTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03704">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01043">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1023" mass="109884" checksum="69D84FFA15F11A3C" modified="2014-01-22" version="1">MDIAVLGPVELRSGSGTAVPVAGARLRTLLVLLALDAGRAVSTGRLADGVWGDDQPAAAGNALQALISRLRRAAPGLAIAATPAGYRLDLDRDRVDAHRFARLVSEGRCDEALALWRGPTEFPDVARADAVRLDELRLAAWQDSAEARLRAGGGAELVAELEAVVAEHPLHEPLAGLLMRALVTAGHPGRALTVFERLRAGLADTLGADPSPELAALHVETLRGQQRRARGNLPAGVSSFVGREDDLRTVRKLIAEHRLVTLTGPGGSGKTRLSVEAGQSLTTVRPDGVWRVELAPVSDPAEVPQAILTALRLRSQIPMIRPGRSPLSESVDPLARLTEALAGKDLLLILDNCEHLIDAAATVADALLRAAPGLRLLATSREPLGIPGEQLWPVEPLALPPTETQAASFPAVRLLLDRAVAARPDFTLDARTTGPVVRICRALDGMPLAIELAAARLRTLPVEVLADRLADRFRLLTNGSRTALPRHQTLRAVVDWSWDLLDDNERALWRRFSIFHGGAEVTAVEQVCGADVDLLGALVDKSLLVLTGDRYRMLETIREYGRNRLAEAGETERMRLAHARYLLELATAAEPELRRSDQLTWLRRLGAEHDNLHAAVRAAIDAGDARTATALTARLGWYWWLRGHRAEGCALARDVLALTGETDPEDRALAYTYAAMNGLEGAAPIDEVVGWFGAAERQGAGPGAAHPVLRLLRPLSAIFQAGGQVREIELVEPLFDDPDPWLRAISKMLVAQLRLNFGQSPDLARAELREALDGFRATGERWGIGFSLSSLGDMAAARGDFEEAVGWQREAITLVREVGIREDLPQLEMKLAQQLWLAGHDTEARRMLEQARESAEDVGLSEVMASVEYGYATLAREEGALDEARRRMARAAELIDASSLAPQFRAMTHSGQALIEGAAGDLAAARDLHVSAVEIAADTRDSPVMAQTLVGVADYALRLGEPARAAMLLGAADAVRGSEDRSVLDTARIRTAARAALGDAGYAEAYRSGADVTLETALAATGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTE3</accession>
    <name>U5VTE3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Excisionase/Xis, DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359674.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09125"/>
    <dbReference type="EnsemblBacteria" id="AGZ40114">
        <property type="protein sequence ID" value="AGZ40114"/>
        <property type="gene ID" value="AFR_09125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1856"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140176_9_1_11"/>
    <dbReference type="OrthoDB" id="2076478at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="17"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="73" mass="8177" checksum="D42B3297D4C7D9A8" modified="2014-01-22" version="1">MAVKTAHVSATAAERLWTVDDVAAFLQVPVATLYQWRHHRTGPPAYKVGRHLRYDPNAVHAWLTEQVGGDDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU24</accession>
    <name>U5VU24_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Enoyl-[acyl-carrier-protein] reductase [NADH]</fullName>
            <ecNumber evidence="9">1.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_11045</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 2,3-saturated acyl-CoA + NAD(+) = a (2E)-enoyl-CoA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:18177"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="2">
            <dbReference type="Rhea" id="RHEA:18179"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:10242"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; mycolic acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.9" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360426.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11045"/>
    <dbReference type="EnsemblBacteria" id="AGZ40498">
        <property type="protein sequence ID" value="AGZ40498"/>
        <property type="gene ID" value="AFR_11045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2250"/>
    <dbReference type="eggNOG" id="COG0623">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_10_1_11"/>
    <dbReference type="OrthoDB" id="762291at2"/>
    <dbReference type="UniPathway" id="UPA00915"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016631">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050343">
        <property type="term" value="F:trans-2-enoyl-CoA reductase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014358">
        <property type="entry name" value="Enoyl-ACP_Rdtase_NADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43159">
        <property type="entry name" value="PTHR43159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000094">
        <property type="entry name" value="Enoyl-ACP_rdct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 9 10" id="KW-0520">NAD</keyword>
    <keyword evidence="6 9 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="20"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="64"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ40498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="255" mass="26824" checksum="1824725544B45749" modified="2014-01-22" version="1">MSGLLAGKRLLITGIITDASIAFSAAKIAQENGATVVLTGFGRMSLVERIAKRLPEPAPVIELDVTSQDDLDALAGKVREHVDGLDGVLHSIAFGPQSVLGGEFLNAGWADVAKALEVSTFSYKSLAMACVPMMGRGGSVVGLTFDATKAWPVYDWMGVAKAGLESASRYLALHLGKQGIRSNLVSAGPLRTMAAKSIPGFVQFEDAWAERAPLGWDLSDQEPTARAICALLSDWFPATTGEIVHVDGGYHALGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU68</accession>
    <name>U5VU68_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0336 protein AFR_04410</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_04410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0336 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358134.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04410"/>
    <dbReference type="EnsemblBacteria" id="AGZ39171">
        <property type="protein sequence ID" value="AGZ39171"/>
        <property type="gene ID" value="AFR_04410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.892"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116276_0_0_11"/>
    <dbReference type="OrthoDB" id="1380653at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00799">
        <property type="entry name" value="UPF0336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039569">
        <property type="entry name" value="MaoC-like_dehydrat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016709">
        <property type="entry name" value="UPF0336"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13452">
        <property type="entry name" value="MaoC_dehydrat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018072">
        <property type="entry name" value="UCP018072"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC_dehydrat_N" evidence="2">
        <location>
            <begin position="9"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="16185" checksum="86490D02CD857CB7" modified="2014-01-22" version="1">MPLDPSFAGRSWPPTEPYAVGREKIREFATAIGATDAEYHDPEAARAAGYTDVVAPPTFPVVVTMAANRQIITDPELGMDYSRVVHGDQKFAYKRPVVAGDSLVCVNYVDEITSRGGHDFLTTRTEVTTEAGEPVVTVWSKLVQRGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUB0</accession>
    <name>U5VUB0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360362.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10875"/>
    <dbReference type="EnsemblBacteria" id="AGZ40464">
        <property type="protein sequence ID" value="AGZ40464"/>
        <property type="gene ID" value="AFR_10875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2215"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_11"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="25932" checksum="DC7116A342D7A373" modified="2014-01-22" version="1">MDGSVLVTGASRGLGAHIARRLAADGRPVAVNYLPDREDAERVVADIERAGGRAAAFRADITDEGDVSALVAAVEAQLGPVAVLVPNATGPQPMIDAVDVTWQDHLDQLTFFVKSPTLLMQAVAPGMRALGGGRIIQIGSDLFERAEAGMSAYSAAKGAQISLTRTWARELGPWGITVNVVAPGWIPVERHAAIEQQYVDAYRQEVPLGRMGTPADIADVVAFLASDGARFITGERITVNGGHTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUG1</accession>
    <name>U5VUG1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative ATPase associated with various cellular activities</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05380"/>
    <dbReference type="EnsemblBacteria" id="AGZ39365">
        <property type="protein sequence ID" value="AGZ39365"/>
        <property type="gene ID" value="AFR_05380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1089"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_3" evidence="1">
        <location>
            <begin position="48"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_lid_2" evidence="2">
        <location>
            <begin position="242"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="36698" checksum="2128903F7CCED440" modified="2014-01-22" version="1">MTDASVEALPPYEVGRLAAAVLDSVGSVVVGKRESLELVLAGILAGGHVLLEDLPGLGKTLTARCFAQALGLDFRRLQFTPDLLPADVTGSFLYDQRKGDFAFRAGPVFTNMLLADEINRTPPKTQAALLEAMQEKQVSVEGVTYRLDPPFHVLATANPIEYEGTYPLPEAQLDRFMLRVSFGYPTQDEEWEVLRRRMSRRQEESQLTPVVNADRLMAMQAALEQVAVEDSIGRYIVALTSATREHASALVGSSPRGSLALLLLARARAVMAGRDYVVPEDVKDVAVPALAHRITLRPEMWLRRVDPSFVVQEVLQAVPTPASGALPTYAGGYAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUI0</accession>
    <name>U5VUI0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_05035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358371.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05035"/>
    <dbReference type="EnsemblBacteria" id="AGZ39296">
        <property type="protein sequence ID" value="AGZ39296"/>
        <property type="gene ID" value="AFR_05035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1017"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_0_11"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="95"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="308" mass="33777" checksum="C29A7B4758B33720" modified="2014-01-22" version="1">MFRYIVRRLLQMVLTFFGATFIVYALMFANQDDPLQALAGERPLTQNVRQALTERYHLDEPFIMQYWYYMKGLLTGDFGTSLTGRKIADMMAQAWPVTIRLALIAIVIAATVAVVAGVVSGIRRGSLFDNVTLVITLIILSLPIVVLAPLAQLIFGIKLGWFPPTAGAHPSFWALLLPALVLGSLVIATELRVTRSSVAENLRSDYVRTARAKGLSRQRVIGVHVLRNSLIPVVTLIGVDIGGLMSGAIVTEGVFNIPGVGFNLFRGIRTEDGPLVVGFVSVLVIVFLVVNLLVDLLYAALDPRIRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUL8</accession>
    <name>U5VUL8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05260"/>
    <dbReference type="EnsemblBacteria" id="AGZ39341">
        <property type="protein sequence ID" value="AGZ39341"/>
        <property type="gene ID" value="AFR_05260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1064"/>
    <dbReference type="eggNOG" id="ENOG50333PP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_523416_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016641">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001695">
        <property type="entry name" value="Lysyl_oxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01186">
        <property type="entry name" value="Lysyl_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665781" evidence="2">
        <location>
            <begin position="22"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="520" mass="55640" checksum="832640952824F782" modified="2014-01-22" version="1">MAGAVATAVGLTAAGMGVANAATDPPPLAFTLATPNVVAERYIWDDDGQAYLYFDLGVNVIAGKDPFEIRATRKSYADPIVANQVVVKNGKKKNVQLPAGMVTGWGGLKDFTTVTIKDAAGAQVANYTTDFCPNSYNSARTRRDAPAENPYPQGCSDYNPFNLGAVWGIQAGWNAATDSMPKGEDFDLADGSYTATVSLNKQYQDYFKIAADSGTLKINVKVETIDERETADGEAVVAKSKAARVGQDTSPEAAVAEHGEHAGQGDPSLQISAYKPNFRPPAKKPKAVKASSIAKGPRPDLKSLPAWGISLNQEGDKSYVAFGATVWNAGTSPLLVDGFRQTGTDLMDAYQYFFDANGKETGSVAAGTMEWDAREGHRHWHFTDFAQYNLLAADQKLAVRSGKEAFCLANTDAVDYTIPNAKWRPENTDLSSSCGQNTAVAVREVLDIGNGDTYTQDRPGQSFEVTDLPNGTYYIETKANPSNKLTELSTTNNTSLRKIILGGTPTARTLEVPPVNGIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV50</accession>
    <name>U5VV50_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40744">
        <property type="protein sequence ID" value="AGZ40744"/>
        <property type="gene ID" value="AFR_12290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2496"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103066_3_0_11"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="13636" checksum="B832D49246D4E620" modified="2014-01-22" version="1">MGGGDVYTVKAIAANTGVALGFIDATVPPGRGSDPHAHRSESEAFYLLSGELEFLDGDRRFTARQGDFVLVPPGISHSFFNRGVHSARMVFMYSPGGPEGMFLDAGDEPRAGESAPAWGPEKIAEVLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV76</accession>
    <name>U5VV76_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell division protein ftsk/spoiiie</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06555"/>
    <dbReference type="EnsemblBacteria" id="AGZ39600">
        <property type="protein sequence ID" value="AGZ39600"/>
        <property type="gene ID" value="AFR_06555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1329"/>
    <dbReference type="eggNOG" id="COG1674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020567_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="705"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="730" mass="79218" checksum="BC083AE12DC8F223" modified="2014-01-22" version="1">MTTTPTTTGVPEDEPERYLRLVPGGLAPEPGTEIGPAVPGSLEPAGDAETHLVGEVIEGVPVDPDDIPLTTTWTDITSRPRVPIIPAWLRDKREFRARVRAVRGLAGYTIAFYATRSPKLAGKVLWYAPKGARRVVGSVIRWAWAEEGNFKLRQHAANTNDAHTWQALDRTRAKSSKARWWLVIPATLALTIAVWVLAASDVVPRLGWWAVIAALLIGLARVGWTGDTPFVDRVSNGPKFTKLTAEMVRKSFTRLGLSQMKELDSMEFVHPGIHRDGPGWLARVNLPEGLEAVKVIEQRGRLSSALRLPVDQVWPAAGPEHAGQVDLWVGYQPASKMGQPKWSLAAPNAVTSVFEPFEFGTDERQRPVKCELFARSWLIGGQPGSGKSYAARSVAAVGMLDPTAEYKIAEFKGTGDFLDLEPICSTYVVGVDDESLKQGAAILVWALGEAEKRGRRILAAKKRGEAPHGKVTPELSRKSGSGLHPVVLLFDEVHELFSAIPEAAANAERLIKRARALNMEIILATQIPDKTSLPPNIVRCVTNRWCLSVAGQQENDMILGTGAYKRGITGTVYRPVQDAGWGCMTGLPTPVSVRSQFPPLDVWDDMVARAIALRHGRAVGATGEQLPAARDLLADLLEVAASNGQHWDKAAPALSERWPQAYPALTQDALSELARALGVESVDVKVAGRNRKGYKSAELRKVIVARDTAEQAPDGPGGGDDRDDEGSTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVF7</accession>
    <name>U5VVF7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP_transf_9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361047.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13510"/>
    <dbReference type="EnsemblBacteria" id="AGZ40988">
        <property type="protein sequence ID" value="AGZ40988"/>
        <property type="gene ID" value="AFR_13510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2743"/>
    <dbReference type="eggNOG" id="COG2343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126578_1_1_11"/>
    <dbReference type="OrthoDB" id="2063459at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007361">
        <property type="entry name" value="DUF427"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038694">
        <property type="entry name" value="DUF427_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04248">
        <property type="entry name" value="NTP_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_9" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="10886" checksum="ADAA638827449B3F" modified="2014-01-22" version="1">MQATIDGTVVADAPESELVTIEGNFYFPPSSLKSELFSNSDTPYTCPWKGVAQYHDVSVGDAVHHDAAWSYPDPYPASFDRVGQDYSGYVAFDKSQVSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVK4</accession>
    <name>U5VVK4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Antibiotic biosynthesis monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360896.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40837">
        <property type="protein sequence ID" value="AGZ40837"/>
        <property type="gene ID" value="AFR_12755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2591"/>
    <dbReference type="eggNOG" id="COG2329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1122720_0_0_11"/>
    <dbReference type="OrthoDB" id="1818589at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="27315" checksum="4B3011FAF36B428F" modified="2014-01-22" version="1">MSINLTLRTSADSNVVALIERLELGENSGQEPVAIPQPPNRTPVRTREQSLALPAVMGTVRGWSRKEGFLGSVVLASRDHNRITVYSQFEPDTGDDAQPALRVLDSIDALGIPARTLDRRTYDLMWRDGNEALTAVSPLHTPVVHFGLFTVLDDQADALLEKVEATAPASLVTPGLRTINFHRSHDRQRVINFGTWSTFEHFHVLHEQPGFTTGEKYWQGLATFENDYFDVIGVIGKNATAGEELLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVP8</accession>
    <name>U5VVP8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13315"/>
    <dbReference type="EnsemblBacteria" id="AGZ40949">
        <property type="protein sequence ID" value="AGZ40949"/>
        <property type="gene ID" value="AFR_13315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2703"/>
    <dbReference type="HOGENOM" id="CLU_1559648_0_0_11"/>
    <dbReference type="OrthoDB" id="2154878at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665768" evidence="1">
        <location>
            <begin position="21"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="18359" checksum="4DF8A3764C54367F" modified="2014-01-22" version="1">MALSLLVLLGGCSGAEQADAPAVATLQSAAAPSSAAAKAGTERPIVPIDATEEDIKVIAQPWVACLAKRGGPDYQDEDAWQWFRKGFDGTDPVMKACLAKMPETQQDVLKRTDMAAFKDNQREFYQCAKREGYKLTAPDPVTGEFGLTSIGPNGDANSAEWQKCEREAFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVU0</accession>
    <name>U5VVU0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative FAD-dependent monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361132.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13935"/>
    <dbReference type="EnsemblBacteria" id="AGZ41073">
        <property type="protein sequence ID" value="AGZ41073"/>
        <property type="gene ID" value="AFR_13935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2827"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_19_5_11"/>
    <dbReference type="OrthoDB" id="504558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="5"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="372" mass="39473" checksum="96988F63CA8A3F48" modified="2014-01-22" version="1">MPDNSVLIAGGGIAGLALERALRRQGVPSALVERDPDGGLGGLAIHLTGNAIAALHTLGLGEGIEQLGRPVRRREYRSNKNRLLCTMDEEALWAPGSRPRLVRRTDLMALLAEAPDGGESRRVATVTSVRPTTAGAAVTFADGHVEEHGLVAGADGVHSVVRTELLGASGTRAALLSTASWRGMAPNPGVDCLTVWMGRGSAAILIPLSDDEAYFYASSVQGGPVENDPSWLGRTFGDFAGPIPEVVDALMADPSRLYHSPVTEVRIPEWSKGRCVLIGDAAHATAPVWGAGAGLAMEDALVLADVLAEGDWDTAGARFEARRRARVGHIAKMTDLTSRGAGTPVWIRGLLLPRTYRATYLPMRRPLDLRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVX5</accession>
    <name>U5VVX5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative carbon-nitrogen hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_07560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359332.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07560"/>
    <dbReference type="EnsemblBacteria" id="AGZ39801">
        <property type="protein sequence ID" value="AGZ39801"/>
        <property type="gene ID" value="AFR_07560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1543"/>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_1_2_11"/>
    <dbReference type="OrthoDB" id="1650683at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="28911" checksum="E722641E2027B3E4" modified="2014-01-22" version="1">MRVAVCQLNARDDRAANLATARELLDRAAAAGADLAVLPEYVDYLGRSAGLPKPEAIDGEFGQFFATAARELGMWVHAGSFHEAGPDDTRTWNTSLIFDRSGQLAATYRKIHLYDVEIPGRVSYQESRTVAPGDKTVVTEIDGVPTGLSICYDLRFPELYRSLAVDGGAKLIVVPAAFMMHTGRDHWEVLLRARAIENQCYVVAAGQIGDHDPGRTCFGRSMIIDPWGTVLAQAPDTTGIVTADIDLARLDEIRAELPSLANRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW60</accession>
    <name>U5VW60_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF559 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08405"/>
    <dbReference type="EnsemblBacteria" id="AGZ39970">
        <property type="protein sequence ID" value="AGZ39970"/>
        <property type="gene ID" value="AFR_08405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1711"/>
    <dbReference type="eggNOG" id="COG2852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_825457_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007569">
        <property type="entry name" value="DUF559"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04480">
        <property type="entry name" value="DUF559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF559" evidence="1">
        <location>
            <begin position="255"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="35782" checksum="3B261A679EBF6B31" modified="2014-01-22" version="1">MTLGSQLARLASDRAIAIQGAELGTIALTLAEDTTAPAAVVARAEAPSATPRDFVAGALEDLERVAVELLPAWLPDSSAIGRPDVGGVAAIRMAATAHARHAHYSPAFLADLAVLAVTGHRNRQPGLPVGVRAAQLARLIAAAFGRPRAALLVPVAAGLSPRDQDVIVAGAGWLVHNARMAVWLIGTLPAGGEQIPSVRVTEAPARRLATIGRPHPNSAVESALEAALAAETWADGRQWNQPYRSTPLSTPVRLDLLWPAERCVVEIDGPEHCHPVHFEEDRRRDVQLQLDGYAVLRFTNARIIHDVGAVVHQIGTYLRRRRGDTAEGNTPWPATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWS0</accession>
    <name>U5VWS0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_09330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AzlC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359755.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09330"/>
    <dbReference type="EnsemblBacteria" id="AGZ40155">
        <property type="protein sequence ID" value="AGZ40155"/>
        <property type="gene ID" value="AFR_09330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1901"/>
    <dbReference type="eggNOG" id="COG1296">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065777_0_1_11"/>
    <dbReference type="OrthoDB" id="1866750at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011606">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34979">
        <property type="entry name" value="PTHR34979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03591">
        <property type="entry name" value="AzlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="25227" checksum="08268F4ED8F61FF9" modified="2014-01-22" version="1">MSTPKRTPDRALLRDAVAIAAAMIAVGASFGAIAIAYGLPTWVPFLMSTAVFAGGAQFLAVGLIAAGNPLAAVFAGLLLNARHLPFGLAVSETVGPRLRDRLIGSHLMTDEVVAFTLAEKDPARRRRTYWLIGITLFTSWNLGTALGVWLGGATGDPAALGLDAAFPAGLIALILPSLRDRDTRLVALTGAAIAVALTPVLPAGLPVLCALLGLLTVVRPRRGNPATSDQTAEHHTKTEEAPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWV7</accession>
    <name>U5VWV7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361418.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15385"/>
    <dbReference type="EnsemblBacteria" id="AGZ41359">
        <property type="protein sequence ID" value="AGZ41359"/>
        <property type="gene ID" value="AFR_15385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3124"/>
    <dbReference type="eggNOG" id="COG1808">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075569_0_0_11"/>
    <dbReference type="OrthoDB" id="1006184at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005240">
        <property type="entry name" value="DUF389"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992">
        <property type="entry name" value="PTHR20992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04087">
        <property type="entry name" value="DUF389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="32017" checksum="392F36BFBB8E7F91" modified="2014-01-22" version="1">MLHVRVIAPGERTPEVLSLLTADTAVTHVVVLAGAAHSPQGDVLEFDVVREGASTVLDQLQELGLDRDGAIVVERVQTSLSASADAAARDTPGLGVDAVVWEEIEQQTGEETTLSASFLAFMIIATVIAGIGVLLDQPILIVGSMVVGPEFGPLAALCVGVVQRKTVLVRNSLLALGVGFPVGMAVTVLAVWLLTAAGLVDKSMLLDERPLTDFIWRPDALSWVVGFLGGVAGILSLTSAKAGTLVGVLISVTTVPAAANVAVAVAYGVRSEASGSALQLVINLAAIISAGVLTLIVQQTVWKWTHRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWX8</accession>
    <name>U5VWX8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Threonine aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_09655</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359884.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09655"/>
    <dbReference type="EnsemblBacteria" id="AGZ40220">
        <property type="protein sequence ID" value="AGZ40220"/>
        <property type="gene ID" value="AFR_09655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1968"/>
    <dbReference type="eggNOG" id="COG2008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029381_0_4_11"/>
    <dbReference type="OrthoDB" id="1124266at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="PTHR48097:SF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_elim_lyase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR017617-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="35019" checksum="D5868BF06A6BD788" modified="2014-01-22" version="1">MADLRSDTVTKPTAAMRTSMAEAEVGDDVFGDDPTVIALERHAAQLFGHEAALFAPSGTMANQIALQLVVPRGLELLAGADAHVVTYELGAAAALGGISTRTWPSVGAVLDVEQITAMIRPAGYPSVPTAAIAVEQTHNLGGGGVTSLETLRALRVAADNAGVALHCDGARIWHAHIADGVPLDAYGSVFDTLSVCLSKGLGAPVGSLIIGSADKIAQARVLRKRLGGGMRQVGILAAAGQYALDNHLTRLAEDHTRARHLAEALEPYGVVEAAQVRTNLVPLDLSKAPLDAPSLAAAAAERGVFIAAMLPRVARLVTHLDLTDADVEKAASVLGELLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX65</accession>
    <name>U5VX65_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15935"/>
    <dbReference type="EnsemblBacteria" id="AGZ41469">
        <property type="protein sequence ID" value="AGZ41469"/>
        <property type="gene ID" value="AFR_15935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3235"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_8_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="2">
        <location>
            <begin position="14"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="95" mass="10075" checksum="A642A1D74BEB6FEA" modified="2014-01-22" version="1">MNPTVSGGKPMDLTTTTQDVKAVLVSVLGIEDRAATIDAATPLLDSLPELDSMAVLELVAALEQRFGVTIDDDEVTAEVFDTLGSLTTLVEDKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXB1</accession>
    <name>U5VXB1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361589.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16160"/>
    <dbReference type="EnsemblBacteria" id="AGZ41514">
        <property type="protein sequence ID" value="AGZ41514"/>
        <property type="gene ID" value="AFR_16160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3279"/>
    <dbReference type="eggNOG" id="COG3324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069623_2_0_11"/>
    <dbReference type="OrthoDB" id="1864858at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="10"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="133"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="28198" checksum="E5FF064B912C8770" modified="2014-01-22" version="1">MTGQRTYPHGVTSWIDTEQPDPRAAAEFYGALFGWTLTAAGPTYLIATLDGKDVGGLAPGGDGQAVWNTYVAVDSADASAEAVTAAGGTVVSPPADPGPAGRAAVCRDPGGAEFRLWQARRRLGAQVTNVPGTWNFSDLHTGDPDAALKFYPAVFGWKAVAMPGAGTMLQVPGYGDHLASTVDPGIYERQAGAPPGFADVIGGLTTTEPGETPHWHVTFTVADRDGSAATAERLGATILGSADEMWAKKALVRDPQGAEFTVSQFAPPEDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXL9</accession>
    <name>U5VXL9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361669.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16580"/>
    <dbReference type="EnsemblBacteria" id="AGZ41594">
        <property type="protein sequence ID" value="AGZ41594"/>
        <property type="gene ID" value="AFR_16580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3362"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_11"/>
    <dbReference type="OrthoDB" id="1261526at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="22703" checksum="6513CFCA91739078" modified="2014-01-22" version="1">MNVLVTGGSSGLGAAVVDAVAKAGGTPYVIDRQAPADGVDWVECDLADTRAAEAATRTVIERAGSLDGVVTAAGMDVPGALADIPAETWERIVTIDLFATAAVIRAALPALVESHGTAVTVASTLGVKAVGDATAYCAAKFGVVGFTRSLAAELAGKVGVTLIVPGGMRTKFFDDRDEQYKPGPDAILNDPANVAAAIMFALNQPAGCAVRELVVAAETETSYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXR3</accession>
    <name>U5VXR3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative RpiR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360479.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11180"/>
    <dbReference type="EnsemblBacteria" id="AGZ40525">
        <property type="protein sequence ID" value="AGZ40525"/>
        <property type="gene ID" value="AFR_11180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2277"/>
    <dbReference type="eggNOG" id="COG1737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055769_0_1_11"/>
    <dbReference type="OrthoDB" id="992687at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05013">
        <property type="entry name" value="SIS_RpiR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000281">
        <property type="entry name" value="HTH_RpiR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035472">
        <property type="entry name" value="RpiR-like_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01418">
        <property type="entry name" value="HTH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51071">
        <property type="entry name" value="HTH_RPIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH rpiR-type" evidence="1">
        <location>
            <begin position="32"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="157"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="33930" checksum="38A66EEEF9F58431" modified="2014-01-22" version="1">MVEPDVDGTATTAVVDSLVRDQAPHVVMNNSDGVLVRVRTLLPEFTGALQRVAEQVLTDPAAASRATIVELAERSGTSPATVTRFCRALGFDGYADLRLGIAAETGRARASGWTVDIGREIQPSDPLERVLGQIMAADTRAMHDTASLLDLAEVERAAVAIASAPRVNIFGASGSALVGEEMQFSLHRIGVAAWAWTDVHNGLASAALSRAGDVALGISHSGETGETIELLAEASSRGATTIALTSFPRSPLAELADIVLVTATQATTFRPDALSARHPQLVVLDLLYVAVAQRTHERSHAAFQRTAQAVHGHKAVKDGSPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXT1</accession>
    <name>U5VXT1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361833.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17005"/>
    <dbReference type="EnsemblBacteria" id="AGZ41679">
        <property type="protein sequence ID" value="AGZ41679"/>
        <property type="gene ID" value="AFR_17005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3451"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102191_1_0_11"/>
    <dbReference type="OrthoDB" id="1497607at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="18085" checksum="71E15EDBAC051737" modified="2014-01-22" version="1">MHLRPYGEADLALTAALEIDPVVMRHLGGAAGDDRVRVVHEKRLAGVAAGDRYRTVTPAGAVSPVGIVAIWRTRWEEESIFELGVMFLPAHQRLGLGLAAARLLLEDFWTAGVSDTVHAFTAVDNVPAGQACIRLGFQLEGDCDLDYEGRPLRCHHWLLTSGGPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXW8</accession>
    <name>U5VXW8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein serine phosphatase with GAF(S) sensor(S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17230"/>
    <dbReference type="EnsemblBacteria" id="AGZ41724">
        <property type="protein sequence ID" value="AGZ41724"/>
        <property type="gene ID" value="AFR_17230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3494"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_32_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="20"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="198"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="409" mass="43854" checksum="EB5A2D2688BEABE2" modified="2014-01-22" version="1">MLTLPGRLDAVRRTGLLDTGPEEPFDRLTRLACELLDAPFGFVTVVDERRSFWKSCVGITSNELADRQNPVGESFCQYIIATDEPVIIGDARLDPMTRDNPSIESMGVIAWAGFPVRSPDGFVLGSFCVVDSRPRQWTAQDLRTLEVLSHAAGGEVALRVAADAASREADDARAAADRYQQLALTMQESLLPRDLPTIDGLDLAAAYRLGGDDVLGDFYDAVQTPTGWALFLGDVAGRGAQAARTSALAKYTLRAGALRASSPAIVLTELDSALHRWYAETTAPGFVTCAYLSLRASEGGFVARICTAGHPAAFIRRADGSVEAFGQPSSPLGILPRLSIRIDETELRPGDLLVLRTDAPIDDAALRAGLGTGPADHAQAVADTLLDHMLQATETPIRDDTVVVALRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXZ9</accession>
    <name>U5VXZ9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2087 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362218.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41873">
        <property type="protein sequence ID" value="AGZ41873"/>
        <property type="gene ID" value="AFR_17975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3647"/>
    <dbReference type="eggNOG" id="COG3860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1438254_0_0_11"/>
    <dbReference type="OrthoDB" id="1782344at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018656">
        <property type="entry name" value="DUF2087"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09860">
        <property type="entry name" value="DUF2087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2087" evidence="1">
        <location>
            <begin position="110"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20090" checksum="2D9EB096EF59F023" modified="2014-01-22" version="1">MSLEAARAADVLAQLSSPVRLLALAELVRRGEDGATLAELRETLDLSLPVAGDALARLVGLGLATGSGSGTYRARPEGLREAAAAVDRTQPIAALLDDYPQLRSNFSHGRLTAMPPTLSDRTPLLGELLARFLDLDGLCDEDRINRRLAEVTDDVAGARRMLVDTGWLERDRAGTTYGPGRPLPQPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY84</accession>
    <name>U5VY84_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_11535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360603.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11535"/>
    <dbReference type="EnsemblBacteria" id="AGZ40596">
        <property type="protein sequence ID" value="AGZ40596"/>
        <property type="gene ID" value="AFR_11535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2351"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_643865_0_0_11"/>
    <dbReference type="OrthoDB" id="1003465at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="324"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="423" mass="44412" checksum="64C60A1996185AF4" modified="2014-01-22" version="1">MRRLSGRFRSALIIGIQGIRARKLRTLLSMVSLFLGVLAVVVVQAGASIAERALFAGVELSNGIDGTKVMDMPPLPQAADIVVDTVRGRSDAMAMLTVSAIIGEPGVTPVNQGAAPFDEDWGGQCYPEQGCDPTQVAPKGQAIEVRLVAMTGDIRPFRPYRPQTGDWLDFSTVPSMAPKIVLNLEAAKGFSRYKVPAEMRVNGASANLTPQLVGVVDDGGYQPQAYVRLDELVTWLPASGLADPSSGGMQVLMAGDTPVEQILITKLRGAGADPYVTTVDSREQGQKEIRLLRLVFLAMAGLVLLIGVAGILNVGLATVGERIEEFALRRAVGTPRSLLAGIVLAETLLTGLLTAAAAIGVSVVGLKALSAFVGESEPFLRDLQFPWDAGVAGIIAGLIAGILGGFVPAVRAARIPIATVMRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYQ0</accession>
    <name>U5VYQ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thrombin-like enzyme gussurobin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18715</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18715"/>
    <dbReference type="EnsemblBacteria" id="AGZ42019">
        <property type="protein sequence ID" value="AGZ42019"/>
        <property type="gene ID" value="AFR_18715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3800"/>
    <dbReference type="eggNOG" id="COG5640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070987_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666223" evidence="3">
        <location>
            <begin position="30"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="30"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="337" mass="35032" checksum="ED5F5B15AF0AFD70" modified="2014-01-22" version="1">MRARFVAMFLLLPWVMAGLLTGSAAPARAIADGQDVAIGKYGFAVKLTMTDLPTTDGGRRDSSCTGALIAPRWVITAGHCFKTARGTRVSRPVARLTTVTAGRTDLTTKAGKVAKVISVKQSPTTDVALAKLEKPITGVTPAQLSRKGPKVGSIVRLAGFGLIADDDESSLATRLQTGQFEVVSRSTAYLGMTGHAPSSTTSPCSHDSGGPYFTQQSDGTAVLVSVVSHGPSCPHSKVDLSGRIDTIRGWITEIVGAEALKPRPSAPAPVRPKPSASTKDRLLTGGSPAESSAPVVAPAAVAAFVLVGFGGVLALVLGGRSRRRAHRRAGVRSHRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYR3</accession>
    <name>U5VYR3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12895"/>
    <dbReference type="EnsemblBacteria" id="AGZ40865">
        <property type="protein sequence ID" value="AGZ40865"/>
        <property type="gene ID" value="AFR_12895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2619"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30896" checksum="C2B880157D5106C1" modified="2014-01-22" version="1">MAAGRKAQARCMTNTARTFLITGVSSGLGRAFAVAALAAGHTVVGTVRKDADAADFSAVQPQRAHARVLDVTDDDAVLAVVGEVEQTVGPIDVLIANAGYGLEGTFEETPLSALRAQFDVNVFGAAATIQAVLPFMRARRRGHILAVTSMGGLAGFPGVSAYCGSKYALEGILESVGKEVAALGIHVTAVEPGSFRTDWAGRSMTRAPRSIADYDDLFDPIREARQEASGRQLGDPARAAAAVLHILDVPEPPHHLVLGSDALRVIRAGRDAVDQDLATWRELSLSTDFPQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZE7</accession>
    <name>U5VZE7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_20505</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362745.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20505"/>
    <dbReference type="EnsemblBacteria" id="AGZ42373">
        <property type="protein sequence ID" value="AGZ42373"/>
        <property type="gene ID" value="AFR_20505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4158"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_61_2_11"/>
    <dbReference type="OrthoDB" id="1384345at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="12"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="404" mass="40781" checksum="A6262267D53FD781" modified="2014-01-22" version="1">MTELSRNHRWAALLALALGGVAIGLTEFAAMGLLPEIARGLLPEAWAESSSDAVARGGWTITAYALGVVVGAPTIAALTARVPRKKLVLGLLVLFVVGTVASALAPTFELVLVARFVAALPHGAYFGAAGLLAATLMGPGSEARGYAAVLSGLTAANVVGVPLITRLGQTSGWRIAYLVIAGVFLLTLAAVLLVVPEVAATPDGSPARELHALRTPQVWLVAATAAIGFAGFFAVNSYIAPVTTEVTGLGAGAVPWVLVAFGLGMTVGNALGGWFADRNLRRAMLIGFACVIASIVLFGLIAATTAGIFIGAFLIGATSLFLGPALQARLITVAPGAQLMGAAVNQSAMNVANSLGAALGGLVIARGYGYLAPTWVGVVLAVAGLGLAAAGFATERRRELSPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZL6</accession>
    <name>U5VZL6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14365"/>
    <dbReference type="EnsemblBacteria" id="AGZ41155">
        <property type="protein sequence ID" value="AGZ41155"/>
        <property type="gene ID" value="AFR_14365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2915"/>
    <dbReference type="eggNOG" id="ENOG50333B5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1792335_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="131" mass="13151" checksum="8766CEEA654D5BB7" modified="2014-01-22" version="1">MALLTATAAHAGFQVTVTVLVYPALARVPAGQWAAAHLAHSRAITPLVVVVYGALVVTGGWSLLSGPDGWTIVALATLAVAALVTAVVAAPAHGRLGDAHDRAIVQRLVRADRVRAAAAALAVAAAALAAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZV3</accession>
    <name>U5VZV3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BlaI transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20150"/>
    <dbReference type="EnsemblBacteria" id="AGZ42302">
        <property type="protein sequence ID" value="AGZ42302"/>
        <property type="gene ID" value="AFR_20150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4087"/>
    <dbReference type="eggNOG" id="COG3682">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119090_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005650">
        <property type="entry name" value="BlaI_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34134">
        <property type="entry name" value="PTHR34134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03965">
        <property type="entry name" value="Penicillinase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="112" mass="12257" checksum="FD1FA406BD4FD13B" modified="2014-01-22" version="1">MTQLWDAGEPLTVRQVHERLSRDRDLAYTTVMTVLDRLAKKGVVLQQRADRAYRYVPAQTREEMTAALMLDALSAAPDGVRDAALAHFVGRIDPTALTAAIEAARKDPGAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W052</accession>
    <name>U5W052_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21210"/>
    <dbReference type="EnsemblBacteria" id="AGZ42514">
        <property type="protein sequence ID" value="AGZ42514"/>
        <property type="gene ID" value="AFR_21210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4301"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_668865_0_0_11"/>
    <dbReference type="OrthoDB" id="870199at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="361" mass="38908" checksum="B837DE70058994DF" modified="2014-01-22" version="1">MLLRGQPPMAAFRIMVEADPQQYGVPMARALLTDVWRSGLDSTVFTEEGIAVASATAPGTPGRTEILLKLYASHQHALFEQGRRAEGMAARAVAGELARTATQHVPRRNGLAQWARGLAEQGRHAEAAELCVEASAAIRLDPVLSGHAMWDEIPRAAQLAAAGRPQEAADVFGAVVSDRRATFPHNRDLVHLLLQQARFLATAGEDPGEAHQEAVDLVRHLAADGETHPSTLHREMWAILAAVSVAGDEMAAGVDPLPAFGWEISEWTPDLRRAYCAGIDDLIAAASRASRPAVLRRRIAARCAVRETWFRKTHDPRTPCAPAFDDSVAAGGPEALIDRAAFAVVRGRYDDALDDLTAALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0W0</accession>
    <name>U5W0W0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phospholipase/carboxylesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21885"/>
    <dbReference type="EnsemblBacteria" id="AGZ42647">
        <property type="protein sequence ID" value="AGZ42647"/>
        <property type="gene ID" value="AFR_21885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4437"/>
    <dbReference type="eggNOG" id="COG0400">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049413_5_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_2" evidence="1">
        <location>
            <begin position="110"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="221" mass="23419" checksum="9A42067C804862ED" modified="2014-01-22" version="1">MADRLAARRPDDRDRLMAYPAPEVAVAGDLGTAGPLVVLLHGRGSHEKEIIALADHLPPGPTYAAVRAPIAEGGGFAWFANRGIGRPLPDSLRLSIDWFRGWLDTLGERPVHLVGFSGGAAFAGGLLLDAPSRFASTAILYGTLPFDAGVPTTPRRLPGVPVFLAHGEQDRVIPPELQARTWSYLHDESGATLTARRDPGGHGIAAPALTALHDWLAARIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0W5</accession>
    <name>U5W0W5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363414.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23165"/>
    <dbReference type="EnsemblBacteria" id="AGZ42903">
        <property type="protein sequence ID" value="AGZ42903"/>
        <property type="gene ID" value="AFR_23165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4696"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092691_1_0_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase_6" evidence="2">
        <location>
            <begin position="8"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Glyoxalase_6" evidence="2">
        <location>
            <begin position="133"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="233" mass="24957" checksum="DF1A6AA8E8EE0AF8" modified="2014-01-22" version="1">MGSHLSAICFLANQPLTLGRFWSGLLGRELVDDNGVVLLPDDTAGFRIRFVETPERKGGQNRAHFDLTSQSSEEQQETVARALELGGRHVDVGQLPEEGHVVLGDPEGNEFCVIEPGNNFLAGTGVIGALAGDGTHAVGCFWSEALGWPLVWDQDEETAIQSPQGGSKITWGGPPLMPRHGRDRVHFDLAPDGDQETEAPRLLALGATRLGTAEDGRLLLADPDGNEFCLLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1V1</accession>
    <name>U5W1V1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dimethylglycine oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_18435</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18435"/>
    <dbReference type="EnsemblBacteria" id="AGZ41965">
        <property type="protein sequence ID" value="AGZ41965"/>
        <property type="gene ID" value="AFR_18435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3741"/>
    <dbReference type="eggNOG" id="COG0404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_11_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032503">
        <property type="entry name" value="FAO_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GCV_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCV_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16350">
        <property type="entry name" value="FAO_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="SSF101790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="2">
        <location>
            <begin position="6"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="FAO_M" evidence="5">
        <location>
            <begin position="353"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="GCV_T" evidence="3">
        <location>
            <begin position="410"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="GCV_T_C" evidence="4">
        <location>
            <begin position="699"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="786" mass="85015" checksum="50AAEBA4AB97280E" modified="2014-01-22" version="1">MDPVTVVIVGAGIVGCGLADELTQRGWTDVTVLEQGPLFTTGGSSSHAPGLVFQTNPSRTMAALARYTVEKLTELDCFDAVGSLEVATTPERLAELHRRHGFATSWGIEAEILGPEACRELYPLLGDVLGGLHVPGDGLARAVAAGEAQARRAVERGARFLPHQRVVAIEQSDGRVTGVRTDDTTYPADVVISCAGFWGPAVGRLAGVPVPLLPMAHQYVRTAPVDAELPATILRHQDRDLYFRSHDDRLGIGSYLHRPMPVDLGDLSEGAQPSMLPFTAEDFAESWTAAGELLPVLAGSEVGEGFNGVFSFTADGFPLLGESREVRGFWTAEAVWVTHSAGVAKAVAEWLVDGRPGIDLHECDLNRFDVSQLSPAYVRERSIRSFVEVYDIIHPLDPPSVRDLRVSPFHTRQAELGAVFGEGAGWERPLWYAANAGLPRDDVERDAWSARNWSPIAAGEARATREGVALYDMTPLTRLEVTGPVSFLQGLTSSNVDRPVGTVVYTLLLDEAGGVRSDVTVARLAEDRFQVGVNGPLDLDWLLRRKPGGVSVRDVTGGTCGVGLWGPHARDLIAPLADIDVSHGAFGYFKARRGHLGTVPVTMLRLSYVGELGWEIYTTAELGLKLWDTLWAAGEPYGIVAGGRAAFNSLRLEKGYRAWGTDMTAEDDPYEAGLGFAVRLDGEDFTGRAALLDRTEPQRRLTCLLLDNEGCVPMGREPVYADGKPVGYVTSAAYGHTIGAPIAYAWLPAAVALPGTSLQIGYFDRRLRATVAEEPLFDPKHERIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2G7</accession>
    <name>U5W2G7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363797.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24705"/>
    <dbReference type="EnsemblBacteria" id="AGZ43207">
        <property type="protein sequence ID" value="AGZ43207"/>
        <property type="gene ID" value="AFR_24705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5005"/>
    <dbReference type="eggNOG" id="ENOG5032X2Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092084_0_0_11"/>
    <dbReference type="OrthoDB" id="1915094at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="25501" checksum="4272C1E16B60C28D" modified="2014-01-22" version="1">MPDDDALITELRALGARLDVPEASDQRDAVRARLTRPDPAPRRVRRWILAGVAALVGAVAVVAPARAAVVDAVGGLLRIAGIEVRQDGDPGSLPATPAPLPSVRNAGLDEVRRTALFPVRLPAELGEPSQVTVADADPSGAPRVISMVFRGGAVRFDQFDGLPSPVFFKMAGDAEWVEIGTGGIWLKKPHPVTYVGRDGAERTETARLAGPTLIWSDGTVTYRLEGPATLAEARRIAGSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2R7</accession>
    <name>U5W2R7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_25205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363897.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25205"/>
    <dbReference type="EnsemblBacteria" id="AGZ43307">
        <property type="protein sequence ID" value="AGZ43307"/>
        <property type="gene ID" value="AFR_25205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5108"/>
    <dbReference type="eggNOG" id="COG3940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009397_2_1_11"/>
    <dbReference type="OrthoDB" id="386120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016828">
        <property type="entry name" value="Alpha-L-arabinofuranosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025414">
        <property type="entry name" value="Alpha-L-arabinofuranosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666325" evidence="4">
        <location>
            <begin position="26"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="2">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="333" mass="36827" checksum="C22587B5340B96E7" modified="2014-01-22" version="1">MMISRRTLLSGSLAMALVPPGPKLAAGPLIEQRADPFITPRIKGRYYFTGSVPEYDRVVVRGASTISGLTTATESVVWRRPATGPMGGHIWAPELHRIDGRWYIYFAAGDSDDVFRIRMYVLESPLADPTDPAGWTLRGRITTEWDSFALDATTFAHRGRRYLVWAQSEPEIAVNSSLYIAEMSSPWALRSKPTRIATPTRSWEIQGYRVNEGPAVLIRNGRMFVTFSASATDARYCLGLLTADARADLLAPSSWAKTPDPVFTTNAQTGQYGPGHNSFTVAEDGVTDLLVYHARDYRDIVGDPLYDPNRHTRVQPFTWNADGTPRFGVPGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Y8</accession>
    <name>U5W2Y8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_26480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364377.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26480"/>
    <dbReference type="EnsemblBacteria" id="AGZ43558">
        <property type="protein sequence ID" value="AGZ43558"/>
        <property type="gene ID" value="AFR_26480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5368"/>
    <dbReference type="eggNOG" id="COG2321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079614_0_0_11"/>
    <dbReference type="OrthoDB" id="1175086at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007343">
        <property type="entry name" value="Uncharacterised_pept_Zn_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04228">
        <property type="entry name" value="Zn_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665643" evidence="6">
        <location>
            <begin position="34"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26483" checksum="EF1D1A8290048A18" modified="2014-01-22" version="1">MPVQRRPHPALLAFVAAALVALGCVSVPEEDTGAPARPAPTTARTTGPAGEDPDGTDTPEEFAADIGDATRVAEEYWKKQFSDSGIDFRPVKRVIPYEEEGEVECGGQPLGLNNAAYCSAGDFIAYDVNWAFGAFRQIGDAFIFYLLGHEYAHAIQLRLRIQKEFTIEQELQADCMAGAYIGDTERKGHLKLDEGDLDELAAGLEAVGDDPGQPWFAEGAHGSAEQRTDAFDNGYRRSLDPCGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W352</accession>
    <name>U5W352_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DoxX family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_20445</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362733.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20445"/>
    <dbReference type="EnsemblBacteria" id="AGZ42361">
        <property type="protein sequence ID" value="AGZ42361"/>
        <property type="gene ID" value="AFR_20445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4146"/>
    <dbReference type="eggNOG" id="ENOG5032SPK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126433_0_1_11"/>
    <dbReference type="OrthoDB" id="2018019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13564">
        <property type="entry name" value="DoxX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13022" checksum="2367B57E254EFB6E" modified="2014-01-22" version="1">MNIALWIVAGLLAVAFLFSGGMKVVRKREDLLKAGMGFVEDFPLGAVRAIGALEVLAAIGLILPAVLDIATVLVPLAATGLALMMVGAIIVHIRRGETKALAPNIVLLLLAVFVAWGRFGPYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3S4</accession>
    <name>U5W3S4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560210.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28060"/>
    <dbReference type="EnsemblBacteria" id="AGZ43873">
        <property type="protein sequence ID" value="AGZ43873"/>
        <property type="gene ID" value="AFR_28060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5688"/>
    <dbReference type="eggNOG" id="ENOG5033Z7B">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_955283_0_0_11"/>
    <dbReference type="OrthoDB" id="1086047at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="33344" checksum="543B34A3CED1D362" modified="2014-01-22" version="1">MTYGKKDLPFPYDQDFSGIVTRVKGIREATRVALVNSTKVASYLKAGANLIEQHFGSDDNAAPADGGRKPYWSGIRFLTERALSREMENLEPPFLRQKGDGPYRSTWASHDDYLNDLLAFLFHDMNYDPQYSAEVETRGGWLRTDGAFGDVLDGAIYDELLVMCRMPLFRLQLLMVATAHRNDGIHAAISSNYEGALAPWKKIYESTIAGRGFQLRKGVTLDDFADTLAAITEGFAIRNLGDPTAGIIGDDPRGHLAGMAVIGILNSYLEPINDPNGLSLREEFEQKSAAARPADRTEDCGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3W8</accession>
    <name>U5W3W8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363046.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22025"/>
    <dbReference type="EnsemblBacteria" id="AGZ42675">
        <property type="protein sequence ID" value="AGZ42675"/>
        <property type="gene ID" value="AFR_22025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4464"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346547_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="1">
        <location>
            <begin position="42"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="240" mass="25887" checksum="65EB834AC27F8C57" modified="2014-01-22" version="1">MVVDHRNPDVLLHGVRLPGPEPVMRLLRRETPPDPALLVLRSVCHELRPPVATLTSLMRALQDQPSEVRRGELARLAAEHASHAQAVLMQAAAAASGLAGPVDEPAPLHRILHSVAVAVPADRLTVAVSRAAGNWPVPARRTRQILLNLLTNAAAYSPGRIRLQGAVRMRRLRLTVADEGALTADLLRALRRPGPPEGTKGLGLWMVRQLTAAQGGTVRARALSPHGVAVEVQLPRKRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4I6</accession>
    <name>U5W4I6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363345.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22855"/>
    <dbReference type="EnsemblBacteria" id="AGZ42841">
        <property type="protein sequence ID" value="AGZ42841"/>
        <property type="gene ID" value="AFR_22855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4632"/>
    <dbReference type="eggNOG" id="COG1238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1452911_0_0_11"/>
    <dbReference type="OrthoDB" id="1849710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666354" evidence="2">
        <location>
            <begin position="23"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="16187" checksum="AC8E595DB6D6B544" modified="2014-01-22" version="1">MIAVLAALAVGFLSAFLPFTPAEPYLIAAVATTGAPPVALGLAAATGQTAGKVLIFLAARGAIRSAWLQRRLTKRRPAAPGRLGRFLARLVAALDRPRQSTGILLASAITGFPPLLAASVYLGRTPMHPLTFAATCLLGRAIRFVTIAFAPHLAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4J8</accession>
    <name>U5W4J8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363455.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23250"/>
    <dbReference type="EnsemblBacteria" id="AGZ42920">
        <property type="protein sequence ID" value="AGZ42920"/>
        <property type="gene ID" value="AFR_23250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4712"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_3_3_11"/>
    <dbReference type="OrthoDB" id="1498264at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="164" mass="18217" checksum="B01A14132D07ED3B" modified="2014-01-22" version="1">MTNPPTGRLVPTATGSDLVLSRTFKAGIEDVWASVTEPGRTARWFGTWTGDAAPGRTIKVQMAFEEETPWMEARVEVCDPPHRLAVSTVDEAGAWHLELLLTSTDGRTELQLVQHLKTTEGLGEVGPGWEYYLDNLVAAREETPLPDFNDYYPSMKGYFEALTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4T5</accession>
    <name>U5W4T5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560466.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44129">
        <property type="protein sequence ID" value="AGZ44129"/>
        <property type="gene ID" value="AFR_29340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5945"/>
    <dbReference type="HOGENOM" id="CLU_1583055_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="17497" checksum="FF70B5D51CE816A7" modified="2014-01-22" version="1">MTVGYSGLVIIARSGDTRLDELACVDDLFVAVTDNVRACGWRIGFLGPVDDSSEGDLAADLALETAAPAIALSVFDSDCAWATAADPSGSTVAFHLNEEIVRILAADDGEDFEPLNSQALAGLLVWVEKAGLVTSPDRLAAALEERPGPFGDGIYAFAEALGIAELPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5C3</accession>
    <name>U5W5C3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase</fullName>
            <shortName evidence="3">GMPMT</shortName>
            <ecNumber evidence="3">2.4.99.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">(1-&gt;4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">glgE</name>
        <name evidence="6" type="ORF">AFR_24190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1-&gt;4)-glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate = [(1-&gt;4)-alpha-D-glucosyl](n+2) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42692"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:10183"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
            <dbReference type="EC" id="2.4.99.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.16" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24190"/>
    <dbReference type="EnsemblBacteria" id="AGZ43106">
        <property type="protein sequence ID" value="AGZ43106"/>
        <property type="gene ID" value="AFR_24190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4900"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015798_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030979">
        <property type="term" value="P:alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02124">
        <property type="entry name" value="GlgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026585">
        <property type="entry name" value="GlgE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021828">
        <property type="entry name" value="GlgE_dom_N/S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF203">
        <property type="entry name" value="PTHR10357:SF203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11896">
        <property type="entry name" value="DUF3416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="5">
        <location>
            <begin position="184"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="240"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Maltose 1-phosphate binding" evidence="3">
        <location>
            <begin position="511"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="3">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="650" mass="73192" checksum="19583644611B5ED1" modified="2014-01-22" version="1">MVDGGERPVKSVVDEEFEVTATVFREGHDAVNATVVLTDPAGRASRAPMTRVNAGLDAWAVTVCADRPGRWTYRVEGWSDPYGTWHHDAVIKVGAGVDVELMLEEGARVLERAVAEVDRTAEQAAVLEEAVTGLRDRRNAPEVRLGAGVTQAVRHELTERPLRDHVSPGTDLPWLVERDRALHGAWYEMFPRSEGATFDETTGLWTSGTFRTAAQRLTAVAAMGFDVVYLTPVHPIGRVNRKGRNNSLEAGPHDPGSPYGIGSSEGGHDAVHPDLGTFDDFDAFVARAGELGLEVALDLALNCAPDHPWVSKHPEWFTTRADGTIAYAENPPKKYQDIYPLNFDNDPEGLYAELRRIVQVWIDHGVTIFRVDNPHTKPLEFWERLIADVAETHPEIIWLAEAFTRPPMMHTLAKVGFQQSYTYYAWRHDRAELEEYCTELAGPAAAYMRPSFWPTTHDILTPFMQRGGVDVWKMRAALAATLVPTYGIYAGYELIENVPRPGAEEQIDNEKYEYKNRRWEDYEPGGPREGQSLAPYLTRLNEIRRAHPALHRLRNLRFHRADDPDVLVFSKSRRIYDRDDVVIVVANLDPSVTRDTWVHLTMPDLGLGWEEGFVAHDLITGQHWNWGEHAFVRVGPDTEPVHIIRVLSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5D6</accession>
    <name>U5W5D6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363785.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43195">
        <property type="protein sequence ID" value="AGZ43195"/>
        <property type="gene ID" value="AFR_24645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4993"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_11"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="8"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="322" mass="35026" checksum="33CB20F3F124883A" modified="2014-01-22" version="1">MTEATLSPLVLGAMMFGTTIDEDTSFALLDRFVERGGVWIDTADCYSFWASKTGRGGDSERVLGRWLAARPGVRDRVRIATKLGAEPLEPGSWPDRREGLSPEAVRAAFAGSLDRLGIDRVDLLWLHQEDRSVPIEDTVEALAALPADRIGVSNHPAWLVERGRAHARAIGVQPFHALQLNATYLRPRPGTLPPGVSHRFGVLSDEQADYATEHGMELWAYTPLLSGAYDNPAKDIAEVYDHPGNAARLAVLDVVVSEVGATRGQVVLAWLLSRGITPMLGGSKLDQLDSAFDGVALQLSADQLERLDTPDRSAWAAPYAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5K7</accession>
    <name>U5W5K7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1616 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363860.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25020"/>
    <dbReference type="EnsemblBacteria" id="AGZ43270">
        <property type="protein sequence ID" value="AGZ43270"/>
        <property type="gene ID" value="AFR_25020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5069"/>
    <dbReference type="eggNOG" id="COG4743">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873271_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011674">
        <property type="entry name" value="DUF1616"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07760">
        <property type="entry name" value="DUF1616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1616" evidence="2">
        <location>
            <begin position="9"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="32850" checksum="7AB3167D745DB08F" modified="2014-01-22" version="1">MNSLTSRIVAALTVASGAAVLLGPVPVAVTGGLLLAFVLPGLALIGVLFRRRELTTVERSVLTPALSMGVLIVAGLVINLVQVRIDRVSWTVSTVAVTLIALLISRRTTVRPATVPVAKPADDSLDALFAGDDTAGEDNAPRPVGEKQVRIDVGEHTVRLPTRSADAGDTVLMSVAPVAADPQSPAPQQRGRLVKQLLPLVVVALILGGASWISFATSRTTHNTTVTALSAAPSGPVTSGGTRTVQVSASGLIAGDGPYTVRVSGSSGRTALERTVAVTGDGTWTERLTLPGAQRLTVNLYRAGDTTAYRTLFISAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5L9</accession>
    <name>U5W5L9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA pyrophosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363806.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24750"/>
    <dbReference type="EnsemblBacteria" id="AGZ43216">
        <property type="protein sequence ID" value="AGZ43216"/>
        <property type="gene ID" value="AFR_24750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5014"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_20_6_11"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="133" mass="14408" checksum="9CE4E46AC77F5EE1" modified="2014-01-22" version="1">MTTEPRVSCVFICHDGDGNLLLARRSAGARDEPGTWDTGAGALEFGETFDAAVTREVTEEYSATPLEITMLGVRNVVRAEPPSHWVAVVFSVRVDPAAVKIGEPHKFDELGWFRPGALPAPLHSQLEPTLALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5V7</accession>
    <name>U5W5V7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase sigma factor sigM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_25300</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363920.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25300"/>
    <dbReference type="EnsemblBacteria" id="AGZ43326">
        <property type="protein sequence ID" value="AGZ43326"/>
        <property type="gene ID" value="AFR_25300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5127"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="20"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="100"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="164" mass="18577" checksum="550155D67EF5A745" modified="2014-01-22" version="1">MSDRDSAFAEYFAARSGVMRGTAYLLCGDWHRAEDLVQAAFTKLYVHWNRVARHEALDPYVRRVLIHTFIDDGRRGWWRRERPSETPGDMPADQPQTDDRLMLMRALAQVPPRQRAVLVLRYWEDMPVEAVAAALNCSAGTLKSQAARGLTTLRGIVAPPVNAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6R9</accession>
    <name>U5W6R9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32220"/>
    <dbReference type="EnsemblBacteria" id="AGZ44702">
        <property type="protein sequence ID" value="AGZ44702"/>
        <property type="gene ID" value="AFR_32220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6522"/>
    <dbReference type="eggNOG" id="COG1916">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009152_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043737">
        <property type="entry name" value="DUF5682"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18934">
        <property type="entry name" value="DUF5682"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="640" mass="68892" checksum="55FB8CFBABEB45B7" modified="2014-01-22" version="1">MDAPAGEGDAPGHGESGAPDLLETSDLGAAPGGEVEAGRRVRPVDPIGELAAAAGYDDPERWWEDVVEHRGMPAFEAVGEAMAAIRETSAEDPEDLVREAYMRTVLREVRKTHENIAVVCGAWHVPALTGKVTAREDAALLKGRKKAKVSFTWVPWTYGRLASWSGYGAGVRSPGWYHHLFTATDEVVPRWLVDAAGVLRAEGVPTSSAHVIEATRLAEALATVRGRPLAGLAEVTEAAEAVMCDGEPLRVALIDRRLVVGERLGGVPEDMPAVPLAKDLAAQQKRVRLKPEALERTLELDLRRDIDLSRSRLLHRLRAIGVPWGEAETARRGTGTFRETWKLRWHPEFAVQLVEGSMWGTTVAAAATTKVIDAARKAATLGDVTELVEVCLLADLTDAYPQVLDALDSRAAVDADINHLMAAIPALARTLRYGDVRRTDVAGLSTVTASLLTRVCAGLPAAVGSLSDEAAKEMRGRIDGVHSAVSLLDDEELRERWLTTLASVSQRADLHGLLAGRLTRLLLDAGRLGLAEVQRRLRLPLTIGTPPARGAAWVEGFLSGGGLLLVHDDALLELLDGWLADIAPEAFDDVLPLLRRTFGGLPPGSGGRSVSGSPGRERPGGPWPRNWCWTTTGRRWCCPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6W2</accession>
    <name>U5W6W2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44742">
        <property type="protein sequence ID" value="AGZ44742"/>
        <property type="gene ID" value="AFR_32420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6561"/>
    <dbReference type="eggNOG" id="ENOG5033QZQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1277012_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="23134" checksum="1F06C056BAFB8202" modified="2014-01-22" version="1">MEGSFVEVADSAASGMDRRAFGEFVGPQGELASYAFGWVTGTEPRVAEFSVGIGAGNPGGGTFHAVVFEREGHYAFSLTDEPFERVPQGGPHLTADQARAHRDLPFVWWVADHVMERDRRARWMQHWVQGTLCFRTPQVSGGHEPILLVTHDADDGVWELVGRAPADDSGKTGHLYHAIDGDPTLIEVLDLPAGHSATRDYPGGPWRRHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W715</accession>
    <name>U5W715_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endo/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560064.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27325"/>
    <dbReference type="EnsemblBacteria" id="AGZ43726">
        <property type="protein sequence ID" value="AGZ43726"/>
        <property type="gene ID" value="AFR_27325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5539"/>
    <dbReference type="eggNOG" id="COG2374">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073780_0_0_11"/>
    <dbReference type="OrthoDB" id="1387978at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19580">
        <property type="entry name" value="Exo_endo_phos_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="189"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="35908" checksum="EE2465B3E1480BCA" modified="2014-01-22" version="1">MAELRIATFNLENFDETAAGDRPSLAERVALMRPQIVRLRATVACFQEVHGQERPGQPRGLHALKELLAGTELAGAELVSTKPKDDGVFNERNLVVATHLPVQSRRQLRNELVSAPTYRRLTAIPADQFPIEVGVERPILHVGLALAGGTLHVINVHLKSKIPTPIPGQAVDTFTWRSADAWAEGVFLSSMKRMAQALEVRRLVDQILDADPDARIVVAGDFNATPDEIPVLAIRGRVEDTGNGDLAGRILVPIEHTVPEPSRYTLFHQGRGEMLDHMLVTRNLLAYYRGSEIHNETLHDESASFATDSKFPESDHAPVVATFDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W860</accession>
    <name>U5W860_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29225"/>
    <dbReference type="EnsemblBacteria" id="AGZ44106">
        <property type="protein sequence ID" value="AGZ44106"/>
        <property type="gene ID" value="AFR_29225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5922"/>
    <dbReference type="eggNOG" id="ENOG502Z954">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033907_1_1_11"/>
    <dbReference type="OrthoDB" id="1555988at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="27960" checksum="E8D1A90E0E5ED4C3" modified="2014-01-22" version="1">MHSNLIFLWGTPRSMSTAFLRMMLERGDHEVFHEPFSSIVVQGRTAVGDYTATSHDELLKLLEERAGDHPVFVKETTEYDYLTTGGDRIPEVGRHTFIIRDPRRVIPSHYAMNPDMACGEIGYGHQVEMARRVRETTGVRPYVVEAEELLTDAPGAVERYCRHLGIPFLESSLSWQPGDQQVWSRTSQWHRDAAGSSHFIRAERTYESTVDNNRFLYDCYWHHRPDYHVLRKWARSHAVSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8K5</accession>
    <name>U5W8K5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <shortName evidence="1">DTD</shortName>
            <ecNumber evidence="1">3.1.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Gly-tRNA(Ala) deacylase</fullName>
            <ecNumber evidence="1">3.1.1.-</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dtd</name>
        <name evidence="2" type="ORF">AFR_35405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DTD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1"/>
    <dbReference type="EC" id="3.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561673.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35405"/>
    <dbReference type="EnsemblBacteria" id="AGZ45337">
        <property type="protein sequence ID" value="AGZ45337"/>
        <property type="gene ID" value="AFR_35405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7164"/>
    <dbReference type="eggNOG" id="COG1490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076901_1_2_11"/>
    <dbReference type="OrthoDB" id="1862614at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043908">
        <property type="term" value="F:Ser(Gly)-tRNA(Ala) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="PTHR10472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="SSF69500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00256">
        <property type="entry name" value="TIGR00256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="short sequence motif" description="Gly-cisPro motif, important for rejection of L-amino acids" evidence="1">
        <location>
            <begin position="132"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15079" checksum="5888F9A7CF1C9D37" modified="2014-01-22" version="1">MRALIQTVSRASVTVDDEVVGKIENGLLVLLGVTHDDTSTIAENLARKVHEMRILDGDASAADTGAPLLVVSQFTLYGDARKGRRPTWSAAAPAEVAEPLVTAFVEALRERGATVETGRFRAHMLVESVNVGPRTVWLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8S2</accession>
    <name>U5W8S2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560678.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30405"/>
    <dbReference type="EnsemblBacteria" id="AGZ44341">
        <property type="protein sequence ID" value="AGZ44341"/>
        <property type="gene ID" value="AFR_30405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6156"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116754_0_0_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="16618" checksum="389AD533AF757671" modified="2014-01-22" version="1">MVVKTYTQAELVAQPIGYWTGAAYRAVVGRIRADLAAEQLAQPHWWILNHVAGAPGTWDRAGLTGKLAPFDDQDTDFAAVFDNLEDRGWLVAAPDGTLTLSEAGEQGRLRARTRGRHAHEQMHEGVTPDEYAATLTVLRRVIDNLGGDSDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8U9</accession>
    <name>U5W8U9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">def</name>
        <name evidence="3" type="ORF">AFR_30555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560708.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30555"/>
    <dbReference type="EnsemblBacteria" id="AGZ44371">
        <property type="protein sequence ID" value="AGZ44371"/>
        <property type="gene ID" value="AFR_30555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6186"/>
    <dbReference type="eggNOG" id="COG0242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061901_1_2_11"/>
    <dbReference type="OrthoDB" id="1649129at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="20083" checksum="ABEC4343D948792C" modified="2014-01-22" version="1">MTVQPIRLFGDPVLRTQADPVTGFDKELRRLVKDLTETMQDEGGAGLAAPQLGVGLRVFAFDVDDVVGHIVNPVLSFPDEEEQDGPEGCLSIPGVYVDTKRRQNVVAQGFNEHGDPIQLIGTGLMARCVQHETDHLDGVLFLDRLDAAARKDAMKQIRAAEWYDAGAPPTVKASPHGRGIFGLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8X8</accession>
    <name>U5W8X8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Shikimate kinase</fullName>
            <shortName evidence="10">SK</shortName>
            <ecNumber evidence="3 10">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">aroK</name>
        <name evidence="11" type="ORF">AFR_30680</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the shikimate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="3 10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560731.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30680"/>
    <dbReference type="EnsemblBacteria" id="AGZ44396">
        <property type="protein sequence ID" value="AGZ44396"/>
        <property type="gene ID" value="AFR_30680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6212"/>
    <dbReference type="eggNOG" id="COG0703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057607_3_3_11"/>
    <dbReference type="OrthoDB" id="1392851at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ44396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="17613" checksum="DF19EF5C4AFCD030" modified="2014-01-22" version="1">MAPVAVVVGPPGAGKTTIGTTVAGLLGVEFADTDHLIEAKAGKPIPEIFFDDGEDTFRALERATISEALGSFAGVLALGGGAILHEGTRDLLREHTVVFLSVGLSDAVKRVGLGAGRPLLTINPRATLKYLMDERRPLYASVATHTIATDARTPDDIAAELVTLLKQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W956</accession>
    <name>U5W956_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31325"/>
    <dbReference type="EnsemblBacteria" id="AGZ44525">
        <property type="protein sequence ID" value="AGZ44525"/>
        <property type="gene ID" value="AFR_31325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6340"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124276_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="106" mass="11674" checksum="CD3B1F73B0CD3DD4" modified="2014-01-22" version="1">MGAKLHHKAGPWPLSLHDSSTVLAVEQNRMLTLTAGLWPLGSARVEVTLEPVGASATRVTLKEDFEAGPLLFVRNKINDLVLHRRNIESLRRLSDIATRAPKEAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Z0</accession>
    <name>U5W9Z0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Succinate dehydrogenase flavoprotein subunit</fullName>
            <ecNumber evidence="5">1.3.5.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sdhA</name>
        <name evidence="5" type="ORF">AFR_38820</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.5.1" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562352.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38820"/>
    <dbReference type="EnsemblBacteria" id="AGZ46018">
        <property type="protein sequence ID" value="AGZ46018"/>
        <property type="gene ID" value="AFR_38820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7855"/>
    <dbReference type="eggNOG" id="COG1053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_8_1_11"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033765">
        <property type="term" value="F:steroid dehydrogenase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="3">
        <location>
            <begin position="11"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="4">
        <location>
            <begin position="488"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="636" mass="70015" checksum="1B62AD374969C3E9" modified="2014-01-22" version="1">MTTRIERHHYDVVVIGAGGAGLRAAIEARLAGKKTAIISKSLFGKAHTVMAEGGAAAAMGNVNSKDNWMVHFGDTMRGGKFLNNFRMAELHAKEAPERIWELETYGALFDRTKDGKISQRNFGGHEYPRLAHVGDRTGLELIRTLQQKIVSLQQEDFAETGNYDSKIRVFSETTITELLLDGNKVAGAFGYYRETGEFILLEAPAVVLATGGVGRSYKVTSNSWEYTGDGHALALRAGATLINMEFLQFHPTGMVWPPSVKGILVTESVRGDGGVLKNTDGKRFMFNYVPDVFRRQYAETEEEADRWYKDPENNRRPPELLPRDEVARAINSEVKAGRGTKAGGVLLDIASRLPAEEVQRRLPSMYHQFKELADVDITKEPMEVGPTCHYVMGGVEVDPDSAAAFGHVEGLFAAGEVSGGMHGSNRLGGNSLSDLLVFGKRAGEHAASYTDALVKRPKVTTSDVESAVDAALAPLSREGGENPYQLQQDLQAVMGDLVGIIRREGELTDALKRLQELKLRVANVGAAGGRKYNPGWHLALDLRNMLVVSECTAKAALEREESRGGHTREDFPKMSPQWRLLNLVCSLDDTGDVHLERKPVPRMRDELIKLFDRTELSKYLTDDELAEYDAVVGEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA51</accession>
    <name>U5WA51_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">S-(Hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_38970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562382.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38970"/>
    <dbReference type="EnsemblBacteria" id="AGZ46048">
        <property type="protein sequence ID" value="AGZ46048"/>
        <property type="gene ID" value="AFR_38970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7887"/>
    <dbReference type="eggNOG" id="COG1062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_14_1_11"/>
    <dbReference type="OrthoDB" id="1859760at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="14"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="361" mass="37138" checksum="CF7415FA6037351D" modified="2014-01-22" version="1">MSAYIQGLVVRDNGAAPAVEELRLPEVGPGQVRIRIRAAGVCHSDLSMVNGTLKPPHPLVLGHEAAGEVVEAGAGVTRVQAGTHVALNWSPPCRQCWFCTHGEPWLCSTANVAALENGITLDGAPVHVTLGLGAFAEQVVVPEKAVIPVPDELAWESAALLGCAVLTGTGAVRNTAEVQPGSSVVVIGLGGVGLSVVAAARAAGAASVIAVDVSEAKKALAEAAGATDFVVSSDNLSKDIRALTSRLGADHAFECVGRAATIRAAWRATRRGGQVTVVGMGRADDMVELSALDIFTSARTLRASVYGSSDPDVEVPALAQDVLSGALKLDHLITDRITLAEVPAAFERMTRGEGARSVVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA83</accession>
    <name>U5WA83_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562272.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38415"/>
    <dbReference type="EnsemblBacteria" id="AGZ45937">
        <property type="protein sequence ID" value="AGZ45937"/>
        <property type="gene ID" value="AFR_38415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7771"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_398300_0_0_11"/>
    <dbReference type="OrthoDB" id="1574675at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="691" mass="76236" checksum="F33C3F877ECBB8C3" modified="2014-01-22" version="1">MTDPADGLSAGETLLLDRLTASLRGVDQITLLLGSGVTAGVIPRVHGVLEIADSFAAGRNDDGALERALEQARSELVGARPIDVYRAYRRVFTDWVSAGAFDVIAQQAVLKAYAAPDPMESPLATHGLGQRLERGVGEGVENDRFSWQLPRGVQALGHLLAQVPEAFDHRVLTTNFDPLLEIAIRSARGRAISLPLDSRGAYGSAGAADGAVRVFHLHGFWRPTLHVRGPRLLHDPARITDNKQLIAATADLIRGDTICVLASSDWSGTVSAALAAVARTRPVRVLWALNDPDAATALSTAEHLREIIGVPVECFPGVDSERLFPALAERAEVPVPPRATGLRHRVRHHSWERQLVSQPGTQPPRDVPGLLLQLERRFGWINQQRGTTSPIRLLWPVRLRSRASVIHMVQAFVAGALARLGVEVRICIDDVGSRDIDVAAEFRADLERWFDHTGHGAVREFVSLTDFLEHVQSQPADTSPEALLRPTDPWSVARTFYGEHNPSLYSLLAAVKAVPNLTSWDELEQNAWPILQSVLRTDANRLLTPLTLWPYLNSMLLESATNDVLTLGGRDEAILWAQWRQTFGFGPGHLYNPYIKSLKHDAHMLRWSSEEELRRHLHSTSELPGWDAEGSYVPWLFQNAMLLPGYLNNDPVPESGDFALDSWAAFVAAIQDDRPVLDQLAARVTDLFLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA88</accession>
    <name>U5WA88_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_38440</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562277.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38440"/>
    <dbReference type="EnsemblBacteria" id="AGZ45942">
        <property type="protein sequence ID" value="AGZ45942"/>
        <property type="gene ID" value="AFR_38440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7776"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_10_0_11"/>
    <dbReference type="OrthoDB" id="785019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13553">
        <property type="entry name" value="PBP2_NrtA_CpmA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010067">
        <property type="entry name" value="ABC_SsuA_sub-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044527">
        <property type="entry name" value="NrtA/CpmA_ABC-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01728">
        <property type="entry name" value="SsuA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666230" evidence="2">
        <location>
            <begin position="24"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="39"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="36545" checksum="5170107C1CA5EFCD" modified="2014-01-22" version="1">MSSRTLRALALAAAAVLTTTTLAACGGDDEASASGEASTIRLGYFPNITHAPALIGVKNGLFQQSLGSTKLEPKTFNAGPAAIEALFSGAIDATYIGPNPAINGWATSKGTALKIIAGSTSGGAGLVVKQGINTPADLKGKKIATPQLGNTQDVALRAWLKANNLNADQQGGGDVSVLPQDNATALQAFAQGAIDGAWVPEPNLSRMLLESKGKLLVNEKDLWPNGQFVTTHLIVKQEFLKKYPATVKKLLQGHIAAVKYIETDNAGAQKAANEQLAALSGKPLKDEILAASFKNLQFTNDPVASSLYTSAQHAQDVGLLKPVDLKGIYDLGPLNELLKADGQPEVSDAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAF7</accession>
    <name>U5WAF7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39200"/>
    <dbReference type="EnsemblBacteria" id="AGZ46094">
        <property type="protein sequence ID" value="AGZ46094"/>
        <property type="gene ID" value="AFR_39200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7932"/>
    <dbReference type="HOGENOM" id="CLU_1583057_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="17051" checksum="63DDBB656B84F26B" modified="2014-01-22" version="1">MTPGQRAGGVVRGLLGAVSLVGGLTWILLNLDAPRPVSDLIVGLVLAAGGLVLLMPHRVDLPGRATAAATAGAAIGGTLAGSVATTITTGGAFVYVASHGWPHAWLARGATAPDPVAARVAANQSSWQADAVNLVADLYFWALVGLLLLAAFVLIRRTGARRQAVLHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAS1</accession>
    <name>U5WAS1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Respiratory burst oxidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_34010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34010"/>
    <dbReference type="EnsemblBacteria" id="AGZ45060">
        <property type="protein sequence ID" value="AGZ45060"/>
        <property type="gene ID" value="AFR_34010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6884"/>
    <dbReference type="eggNOG" id="COG4097">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_19_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="7">
        <location>
            <begin position="277"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="502" mass="55687" checksum="19A57175395FE7D1" modified="2014-01-22" version="1">MFQSFEDQREPSFGQTLAPERDFWSQPDLNEGVPRGDIVVEAPQDPSGWQTSGPPISTDDRPPAEATSGNRLLTVLLFFAGLATSVSLWLFDTQAGAINDTATLMMAVGRITGLIAGYLLFIQLLMMSRVSWLEEWVGARDLLRWHRWLGTSLVVTVLAHIVFIVYGYALTAESGVVDQAWTIITTLPEMISATVATGLLVLISVISIRAARSKLPYELWHFIHLTAYLVLLLGYGHQVATGANLSGRFASVFWPALSALVIAALVWGRLVEPLWLNARHRFEVAEVVAEGANTFSIYIDGRRLESLPAQAGQFMRWRFLTANGWWQSHPFSLSAAPNSEWLRLTVTAVGGHTSKLAEMEPGTPIWAEGPFGTFTAQRRTRRRALLIAGGSGIAPIRALLEDMPPDTIVIYRASRPDELVFREELEDLAERRDAWVRYIVGSRNDPGPRRLFTATGLLGLVPDVNRRDVYLCGPPGLVDAAVQTLRELEVPDSQMHLDPFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAU3</accession>
    <name>U5WAU3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Two-component system histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33940"/>
    <dbReference type="EnsemblBacteria" id="AGZ45046">
        <property type="protein sequence ID" value="AGZ45046"/>
        <property type="gene ID" value="AFR_33940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6870"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021898_0_0_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="550"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="731" mass="75165" checksum="CA37FA4B5E0381B5" modified="2014-01-22" version="1">MIAVGRMRRRALLPVLVYATAVTLLAGLTFSPARPLSPVEWATDSVLLGVPVVIGLLLSWRVRTGPVGAALSWVGAAPAVVFTIEGWGETTLTADPWPAATWFAVVQSGVWVWNLAGFVSLCLVFPDGLLPGRRWRGVIAFCVVAGISVNLTISLLQPQSNEPGVFAIRVPDAVFFGLLVPALCSVLAAVALAVTSLVIRYRRGGEMLRTQLRWLLLGALTVPVLLAAGWIAEILGAPTGVAYVGFLAMMVLAVPGAIAIAVVRHDLFDVDRLLGTTLAWLGTSVVSAAIFAAVVAGVAQIAGPDSTAGLTGAAFVTAMCLLPLHRLLNELVGRVVDRDGTVSRARIEQFVRRVRGGEAEPEETESVLRAVLRDEGLRLLVRLPGSPPGVYVDLAGRPAELGPPSEPVHLAGPGVPAPPGVAAGPGLAAGPGLAAGPGLAAGPGLAAGPGLAAGPGLAGQAQAPVGAGLSARLSGGVGAEAVRVPLRTGDSEVGVIVLGISSARRLRRAREAALQARLPIEVSRLRLELRLALDDARAGRSRLALAGAAERHRLARDLHDGAQQQLIAVGMRLRSVQRGIDPALTAHQDLEAAVSTLAATVADLRRLAHGIRPARLEDGLAAALRALAADSSVPVSLVVPELDPSEAVATTAYFLVAEALTNAMKHARATGISVVVEPCGPGLRIEVTDDGVGGTSPGFGLTSVRDRVTSLGGELTVDSPPGGGTRIEAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAV5</accession>
    <name>U5WAV5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative precorrin methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_33990</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561393.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33990"/>
    <dbReference type="EnsemblBacteria" id="AGZ45056">
        <property type="protein sequence ID" value="AGZ45056"/>
        <property type="gene ID" value="AFR_33990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6880"/>
    <dbReference type="eggNOG" id="COG1010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029920_1_0_11"/>
    <dbReference type="OrthoDB" id="1195697at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006364">
        <property type="entry name" value="CobI/CbiL/CobIJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01467">
        <property type="entry name" value="cobI_cbiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="8">
        <location>
            <begin position="4"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="TP_methylase" evidence="8">
        <location>
            <begin position="259"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="503" mass="53543" checksum="80A4F1F0C736C971" modified="2014-01-22" version="1">MAGRLFGVGLGPGDPELVTVKAARLIGAADVIAYHAARHGRSNARAIAESYLREGQIEEPLIYPVTTETTDHPGGYQGAIDEFYAESAERLAAHLDAGRDVVVLAEGDPFFYGSYMHMHKRLADRYEAVVVPGVTSVSAASAVLGRPLMERDETLTVLPGTLPPAELAARLAGTDSAAIMKLGRTFPGVRDALEQAGRLDDAWYVERATTDRERSGKVADIDPSTVPYFSLALLPSKAALTGTAAPTETPVSQDGRGEVTVVGLGPAGQEWLTPEAQAALAEADDVVGYGPYVDRVPDSPHRRKHPSDNRVEAERAAFALDLAKRGRRVAVVSSGDPGVFAMAAAVLEVSEDPQWKDVPVRVVPGLTAAQAVASRAGAPLGHDFCIMSLSDRLKPWEVIAGRLTAAASADLVIAIYNPASRSRKEQLVKARELLLEHRTPQTPVVVGRDVGGPEESILVTTLGELDPATVDMRCLLIVGSSQTRMTTRGDGSTQVYTPRHYPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB06</accession>
    <name>U5WB06_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45111">
        <property type="protein sequence ID" value="AGZ45111"/>
        <property type="gene ID" value="AFR_34265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34265"/>
    <dbReference type="eggNOG" id="ENOG502ZJPF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1795427_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="16859" checksum="28D4CF806467B369" modified="2014-01-22" version="1">MPTTDEAMLPGVTVVVETFLLGTTGGRLTYRTASRALTDDEHPDAVARALAGVAADGLLHSTSWRFASGRIVLTYAALPDPDPGSATVVRAPGQTLAAGDGPLSPSPGRVDADDVAVHACRHLAFLRRTDPVVKASAARHRVLWDLLDEHQPAVAGLLRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBM4</accession>
    <name>U5WBM4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative M28-family peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_35720</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561736.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35720"/>
    <dbReference type="MEROPS" id="M28.003"/>
    <dbReference type="EnsemblBacteria" id="AGZ45400">
        <property type="protein sequence ID" value="AGZ45400"/>
        <property type="gene ID" value="AFR_35720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7230"/>
    <dbReference type="eggNOG" id="COG2234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024336_2_0_11"/>
    <dbReference type="OrthoDB" id="1465483at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03876">
        <property type="entry name" value="M28_SGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029514">
        <property type="entry name" value="Ape3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041756">
        <property type="entry name" value="M28_SGAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="PTHR12147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF17">
        <property type="entry name" value="PTHR12147:SF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666266" evidence="3">
        <location>
            <begin position="24"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M28" evidence="4">
        <location>
            <begin position="96"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="506" mass="51693" checksum="78FB31FA4DB37C45" modified="2014-01-22" version="1">MRAVPFIGATTLVAALFAVPAQAAPTVALAPPNIPVANVKAHLSQLQSIATANGGNRAHGRPGYLASVTYVKGLLDAAGFTTQQQSFTYGGATGYNLIADWPGGDTTDTLMIGAHLDSVTTGPGINDNGSGSAANLEVALQVARSNFQPQRHLRFGWWGAEELGLRGSTAYVNSLTSAQKANIGGYLNFDMVGSPNPGYFLYDGDNSDGTGSGPGPAGSAEIEDTLEAYFSSISVPTRGTDFDGRSDYGPFIAAGIPAGGIFTGAEGRKTSAQVSLWGGTTGAFDPCYHAACDTISNINDTALDRNSDAIAYSVWTLADGTTEPPPTGETVYSDTFETATGWTVNAGATDTATSGRFERGDPAATSSGVATQLGTTVSGSYDLVTGATAGASAGANDVDAGTTTVRSPAITLPSGTLNLSFSWYLSHLNNATSGDYFRVRVISGTTTTTVFTQAGAASNRAAAWQTATVNLSAYAGQSVRLQVEAADTGTASLVEAAVDNVLITQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBN9</accession>
    <name>U5WBN9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46412">
        <property type="protein sequence ID" value="AGZ46412"/>
        <property type="gene ID" value="AFR_40790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8258"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_7_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="9"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="142"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="328" mass="34590" checksum="43D35139D86F2F89" modified="2014-01-22" version="1">MAIMTEKAVRWGILGTGGIAATFAADLPLVPGAELAAVGSRTPESAATFANRHGFARSHGSWAELAADPDVDVIYVATPHAAHLDAAMTCLAGGKAVLCEKPMTLDVAGSQQLIDEARNRGVFLMEAMWMRMNPAIRRIVALVRDGAIGEITNIQADFGLQGPFAATHRLRDPALGGGALLDLGVYPLNLAHLILGAPATTRSWAHLTPEGVDDNTGILLGWESGAIASLTCSLIGETRNAASITGTLGRIDLPQGFFVPRSFVLHRAGRAAEEITEPFPGSGYQYEAGEVQRCLAEGLLESPLMPHATTLEIMGLMDSIRAEIGVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC09</accession>
    <name>U5WC09_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MarR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562990.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42025"/>
    <dbReference type="EnsemblBacteria" id="AGZ46659">
        <property type="protein sequence ID" value="AGZ46659"/>
        <property type="gene ID" value="AFR_42025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8504"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_15_0_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="16768" checksum="31423173C73D7AA0" modified="2014-01-22" version="1">MVDDDAGSTARQELMACMREMLKAVRLFKSDLPVEHTTVPVGALAMIDSAGDCHLKDLAARSALDPSTVSRAVATLVKSGLVGRSADPADGRASVLALTPEGRRTLDEVNGWMDDRLADALKDWSPEDIAVFSALMQRFATDLMTRHDQTLEVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCU4</accession>
    <name>U5WCU4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AFR_37855</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37855"/>
    <dbReference type="EnsemblBacteria" id="AGZ45825">
        <property type="protein sequence ID" value="AGZ45825"/>
        <property type="gene ID" value="AFR_37855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7655"/>
    <dbReference type="eggNOG" id="COG0266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038423_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="DNA_glyclase/AP_lyase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015887">
        <property type="entry name" value="DNA_glyclase_Znf_dom_DNA_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="MutM-like_N-ter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000214">
        <property type="entry name" value="Znf_DNA_glyclase/AP_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="SSF81624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01242">
        <property type="entry name" value="ZF_FPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51066">
        <property type="entry name" value="ZF_FPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="17" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="17" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FPG-type" evidence="16">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ45825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28885" checksum="B46AED30C720A4D0" modified="2014-01-22" version="1">MAARHRALFAGRVVAVDSPQGRFEAGAALIDGRRLVDTEAYGKHLLHHFEGERTLHIHLGLYGKFADGEGSLPVPVGQLRMRMADARHWLELRGPTACEVLDPGLVAVLRARLGQDPLRDDADPRAVYPRVAASSKPIFALLMDQTIVAGCGLIFANEVLFRAGLAPTMPGRSVTLRCWDELWDDLRGLMKEGVVRGRIDTVHTAHTPESMRRAPRVDRHGGEVYVYRRTGQPCLVCGTPIVRGPLAGRNLYWCPTCQASQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCW0</accession>
    <name>U5WCW0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42790"/>
    <dbReference type="EnsemblBacteria" id="AGZ46812">
        <property type="protein sequence ID" value="AGZ46812"/>
        <property type="gene ID" value="AFR_42790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42790"/>
    <dbReference type="HOGENOM" id="CLU_2152889_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="111" mass="12677" checksum="A8A5EFD4F9EB8D5F" modified="2014-01-22" version="1">MVLVRIDYQLVLKADLSGEITSLIAYQALPDKPFQAVLWSVRRTAWIYAPGLAVPMLYDDKYQDRTRVIDRAKAEKISRESLSTELPSEATLQSMCEEGERMGWNYGPPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCW8</accession>
    <name>U5WCW8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endonuclease IV</fullName>
            <ecNumber evidence="9">3.1.21.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_42840</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.2" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563152.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42840"/>
    <dbReference type="EnsemblBacteria" id="AGZ46822">
        <property type="protein sequence ID" value="AGZ46822"/>
        <property type="gene ID" value="AFR_42840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8666"/>
    <dbReference type="eggNOG" id="COG0648">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025885_0_2_11"/>
    <dbReference type="OrthoDB" id="1088517at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008833">
        <property type="term" value="F:deoxyribonuclease IV (phage-T4-induced) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="PTHR21445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="8">
        <location>
            <begin position="13"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="27293" checksum="F72084A14208FD67" modified="2014-01-22" version="1">MRIGAHVDPAEPLDAAAARGADAVQFFLADPQGWKSPAARPDAEALKTSDVDVYIHAPYVINVATLNNRIRIPSRKLLLAHARAAAAVGAKGLIVHGGHVNAGDDIAVGFDNWRKTFAYAEKEGGLPLPILIENTAGGDHAIARRFDNLKRLWDAVGEFGAGFCLDTCHAHAGGEELLDIVDRVKSITGRIDLIHANDSKDTFDSGRDRHDNLGNGKIDPELVVAAIRAADAPAIVETPGGVEGQTADIALLREKAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD06</accession>
    <name>U5WD06_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38230"/>
    <dbReference type="EnsemblBacteria" id="AGZ45900">
        <property type="protein sequence ID" value="AGZ45900"/>
        <property type="gene ID" value="AFR_38230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7734"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072253_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025847">
        <property type="entry name" value="MEDS_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14417">
        <property type="entry name" value="MEDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MEDS" evidence="2">
        <location>
            <begin position="15"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="1">
        <location>
            <begin position="205"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="34355" checksum="D02FC2BDDEED694F" modified="2014-01-22" version="1">MRGSDATLVRPRFLHSAFFYDTDDAYSIALAAFVREGLARDEAVAVVSGRDHTGLLRDALGEDATEVRFLPAEDWYVRPVRTIAGWAHLLKATTASGRSGTRLVGHAPFTQGLQSWVRFESALNRSLDGLPGHLLCPYDRGALPDDAVLGAGRTHPRLHDGGDWVDSTGYESPEQLLIEVPEPPYPVAGEPIIAVPVTEAVADLRAQVRGRADAEGWLSPDRVENLVLALTEVATNGVRHGGEHRELRVWLTDTAVVCEVTDDGRVPPGPLAGYLPPRPGMIGGMGLWLVQQLCDAMAIHSADGVTRARFALRRDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDE2</accession>
    <name>U5WDE2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562300.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38560"/>
    <dbReference type="EnsemblBacteria" id="AGZ45966">
        <property type="protein sequence ID" value="AGZ45966"/>
        <property type="gene ID" value="AFR_38560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7802"/>
    <dbReference type="eggNOG" id="COG0645">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106286_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="172" mass="18260" checksum="B19BC64C90B8A314" modified="2014-01-22" version="1">MARDVVMVNGLPGSGKSSLAPRLAAALGAACLEKDRIKEALAEAVVTEVPDLGAVAMETVWSLAAAVSGMAVVDSWWFRPRDLDHARTGITRTGATATVEVWCDVPPELARARFIARKRPGVHADTERLATQWAGWATQAEPLALGPVLRIDTSRPVDIPALSTAVKAALWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEJ8</accession>
    <name>U5WEJ8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562802.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41080"/>
    <dbReference type="EnsemblBacteria" id="AGZ46470">
        <property type="protein sequence ID" value="AGZ46470"/>
        <property type="gene ID" value="AFR_41080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8315"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145408_0_0_11"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="15241" checksum="7843827E0A7E3023" modified="2014-01-22" version="1">MRIERVEDAAAVHRAADLFDGPPLAEATEKFLAHPDHHLLFAYDGDRAVGMVSGVEMTHPDKGTEMFLYELGVAPTARLQGVGSALVEALAAVARERGCYGMWVGTEVGNEAAQATYRRAGATEETPFVLLNWDLAPGDQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFG1</accession>
    <name>U5WFG1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bacterial proteasome activator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_42605</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Forms a homooligomeric, either hexameric or heptameric, ring-like structure which stacks co-axially with the proteasomal alpha-rings.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Bpa family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563106.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42605"/>
    <dbReference type="EnsemblBacteria" id="AGZ46775">
        <property type="protein sequence ID" value="AGZ46775"/>
        <property type="gene ID" value="AFR_42605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8622"/>
    <dbReference type="eggNOG" id="ENOG502ZPJ4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111456_0_0_11"/>
    <dbReference type="OrthoDB" id="1767315at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019695">
        <property type="entry name" value="Proteasome_act"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10759">
        <property type="entry name" value="DUF2587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="158"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="191" mass="20492" checksum="B06FE7290A96862A" modified="2014-01-22" version="1">MTDDTRTPDQQEDGSVIVVGPDGRPLGTVESDGTLSPEEPGNLIEQPAKVMRIGSMIKQLLEEVRAAPLDEASRTRLREIHQRSIKELEDGLAPELQEELERLSLPFEGETPPSESELRIAQAQLVGWLEGLFHGIQAALVAQQMAARLQLEQMRGGNAGRPALPMGPNGVIPGMPGQGGGDGSGRTGQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFR4</accession>
    <name>U5WFR4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563102.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42585"/>
    <dbReference type="EnsemblBacteria" id="AGZ46771">
        <property type="protein sequence ID" value="AGZ46771"/>
        <property type="gene ID" value="AFR_42585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8618"/>
    <dbReference type="eggNOG" id="ENOG5031MNG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1222642_0_0_11"/>
    <dbReference type="OrthoDB" id="2090260at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="26594" checksum="58B2E9B6BC31BC81" modified="2014-01-22" version="1">MPETPMEAVVEFGPDDDAAREPRRFNPGGFLTGLAADRRLVPIAAVLGAVALFGSFVSEWQITEVDRSVFGDGEVGTRPLETSLADLGSWGGGYLAALFLLVPLVVLTLFGPFAGRRYTRLGALSVGGVLLAMLAATGSELGDVSRIIGQPGLSGLDADQVTISYGRGLWCAVAGTVLVVLAMYLAGRDTDVPPPLSDDDEPAPEPPPIWSWRRPEAAEEGPPDAPFDLTVTSTKPFTASQDNRDKPSEGISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNG4</accession>
    <name>U5VNG4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01095"/>
    <dbReference type="EnsemblBacteria" id="AGZ38508">
        <property type="protein sequence ID" value="AGZ38508"/>
        <property type="gene ID" value="AFR_01095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.218"/>
    <dbReference type="eggNOG" id="COG0510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068889_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="112"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="33178" checksum="3D75D754665A85F2" modified="2014-01-22" version="1">MRDAAILRRMLSEPEGLTIAELLAGLAQGWGINARSAEYLPVGFGSYHWLVAGHFVTVDALEDEAAYTSLCRALETARALHLDFVVAPLPTTAGPVVWRLGPRYALSVYPVVEGDAGDFGPHRPDDLPEVLELLTALHRAPIPDCTERADLALPGRSALAGLDSPWTGGPYSERARHLLVEHADDLAALLAEYDALVEQVRPTGARWVVTHGEPHPGNFLRTPAGLVLVDWETARIAPPERDLWFVVETAGDPVALALYRLRWKLADIAAYAGEFRRPHEITDDITASWTYLQGYFPLRSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNI3</accession>
    <name>U5VNI3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_00340</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00340"/>
    <dbReference type="EnsemblBacteria" id="AGZ38357">
        <property type="protein sequence ID" value="AGZ38357"/>
        <property type="gene ID" value="AFR_00340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.70"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="10">
        <location>
            <begin position="5"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="226"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="291"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ38357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="350" mass="37625" checksum="628D1BB46EDB8A2F" modified="2014-01-22" version="1">MWSRRRSPPMTELLVTVVLLLGNALFVGGEFALIASRRTALEPLAATSRRARWALSAMNQIPLMIAGAQLGITICSLGLGAIAEPALAHLVEGPFEAIGMPERAVHPVAFVLALGVVVFLHTVVGEMVPKNITLAGPERSALVLGPFMLAFCTATKPILTAMRWAAKIVLSLWKVEATDAVKTVFTAEELAGMVTQARAEGLLGTEQYARIHAALGLNVRTAADTLQPWSGVTTVADDASPATIEAIATRSGRSRFPVVQRETRRVLGFVHVKDILGYEGAQRRLPIPAEIIRPLAVVPPERTLADLLLTMRRDRRHIVLVSDGRRPLGVIALDDVLHAVVGEPSVRSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPE6</accession>
    <name>U5VPE6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Response regulator gacA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_02770</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357786.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02770"/>
    <dbReference type="EnsemblBacteria" id="AGZ38843">
        <property type="protein sequence ID" value="AGZ38843"/>
        <property type="gene ID" value="AFR_02770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.556"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="144"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="23452" checksum="0DC6CD7AE18343F3" modified="2014-01-22" version="1">MRVVIADDAVLLREGLVRLVEENDCTVVAAVGDGPSLVQAIAEHRPDVSIVDVRMPPSHTDEGLRAAVEARGKVPGSPVLVLSQYVEVEYADDLLADRLGAVGYLLKDRVSEIAEFMDALRRVAGGGTVLDPEVVGQLLVRRRRDDPLRNLTPREREVLGLMAEGRSNTAIARKLVVTEGAVEKHVRNIFTKLDLPPDTEQHRRVLAVLAYLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPH8</accession>
    <name>U5VPH8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadK</name>
        <name evidence="6" type="ORF">AFR_02920</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357816.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02920"/>
    <dbReference type="EnsemblBacteria" id="AGZ38873">
        <property type="protein sequence ID" value="AGZ38873"/>
        <property type="gene ID" value="AFR_02920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.587"/>
    <dbReference type="eggNOG" id="COG0061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_0_0_11"/>
    <dbReference type="OrthoDB" id="1463423at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="73"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="145"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="32048" checksum="3826564FF119B560" modified="2014-01-22" version="1">MSLDTAVDTVGLVLHPSKPVGDSISTIADWARGHQGRVLARTSDAGRVPDDVEVVPDDEFLARVNGVVALGGDGTMLGAMRLVIDRPVPVLGVNHGNLGFLVEVTPSTLGAALEQLASGDFTVESHSCLTADAGEAALATRFGFNDVVLARLGRTGAVSVDLTLNGRQYGYYKCDAVVVSTPNGSTAYNYAAGGPILSPSASAMVITPVAPMAGIGRAVVLGAGDRVALTVAGDSAPVAVDVDGTASGELAAGAVLSAGLREQAAQVVRLSEGAHQNRNSVKLSLLDLPLRRDQLLELIPERLRENAQLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ58</accession>
    <name>U5VQ58_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03315"/>
    <dbReference type="EnsemblBacteria" id="AGZ38952">
        <property type="protein sequence ID" value="AGZ38952"/>
        <property type="gene ID" value="AFR_03315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.667"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088572_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041484">
        <property type="entry name" value="TetR_C_25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17933">
        <property type="entry name" value="TetR_C_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="22655" checksum="5E204D699B5F294E" modified="2014-01-22" version="1">MSSDLTARARIRDSAIRLFAEKGIVPTTIRDIAHEAQVSSGLLRHHFGSKDGLRDACDEFALGRMTVIRTAMIARGGMADAAFVGTVHPESMLLQRYLVRSMMDGSASATAMFEKLVEVGEQWLADQPIESKDPRVYATALVAMQMGMFMMRDQMAQVLGVDLNQPANHARLLRATVEIFAQPLLDPTQADQAYVTLDRLTAAEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRL4</accession>
    <name>U5VRL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359035.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06075"/>
    <dbReference type="EnsemblBacteria" id="AGZ39504">
        <property type="protein sequence ID" value="AGZ39504"/>
        <property type="gene ID" value="AFR_06075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1234"/>
    <dbReference type="eggNOG" id="ENOG5032IZH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2165535_0_0_11"/>
    <dbReference type="OrthoDB" id="1964475at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="110" mass="12217" checksum="5E210EE765E56785" modified="2014-01-22" version="1">MDNAELARLVEAEHPYRGKALFELSDRIAADDDAATKVAMLSRLTSLRTARLFDRVSLAWSAIIALLAAETPHSRSSAYEAFYALGPEEQKDMLDYLEVPVIEDAHPRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRN4</accession>
    <name>U5VRN4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_05940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359008.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05940"/>
    <dbReference type="EnsemblBacteria" id="AGZ39477">
        <property type="protein sequence ID" value="AGZ39477"/>
        <property type="gene ID" value="AFR_05940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1207"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_0_1_11"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="21644" checksum="42C4F70213A1ABDB" modified="2014-01-22" version="1">MGTEETWSVGELAAATGLTVRTLHHYDEIGLARPSRRSAAGHRRYTDSDLRRLHRIVALRGFGFGLAEIAALLDGAGPDPRELLQGQLDQVRDRIARARRLHSRIEQVLGQFDAEGDPSAATLIRLIEEMTAVEHTYTPQELEAMAARRQELMAQLSPEQLAEMAETRRRLRESLTPEQLAELERSRPRLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRT8</accession>
    <name>U5VRT8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a quinol + H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = a quinone + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:39663"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the F420H(2)-dependent quinone reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359209.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06945"/>
    <dbReference type="EnsemblBacteria" id="AGZ39678">
        <property type="protein sequence ID" value="AGZ39678"/>
        <property type="gene ID" value="AFR_06945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1417"/>
    <dbReference type="eggNOG" id="COG3945">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114921_2_0_11"/>
    <dbReference type="OrthoDB" id="1693553at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004378">
        <property type="entry name" value="F420H2_quin_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04075">
        <property type="entry name" value="F420H2_quin_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00026">
        <property type="entry name" value="hi_GC_TIGR00026"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="15389" checksum="87F327CA13429EBC" modified="2014-01-22" version="1">MTMPDDLLAHNRQLIDQFRADGGASMTNRPLLLLTTAGRRTGQPRTSPIMYVRHDDRLLVIASNKGAATDPLWFRNLIADPVVTVELPGESFTATATPLTGDDYDAEWARLVSAYPFFAEYQAKAGDRTIPLVELSRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRU0</accession>
    <name>U5VRU0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_00460</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0073 (Hly-III) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357325.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00460"/>
    <dbReference type="EnsemblBacteria" id="AGZ38381">
        <property type="protein sequence ID" value="AGZ38381"/>
        <property type="gene ID" value="AFR_00460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.94"/>
    <dbReference type="eggNOG" id="COG1272">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051078_2_2_11"/>
    <dbReference type="OrthoDB" id="1659567at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019835">
        <property type="term" value="P:cytolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005744">
        <property type="entry name" value="Hy-lIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="PTHR20855"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01065">
        <property type="entry name" value="hlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="24772" checksum="0AED5FF4687EBBEC" modified="2014-01-22" version="1">MTTSAPFKMKRVDLGKPRLRGRLHQYAFFVALVSGVVLCSIAATRPGVAPFLSTLVYSITVCGLFGISALYHRLEWTERGYQIMRRLDHSMIFIFIAGTYTPFCILLLDRPKATVMLSLIWTGAIGGVALKSIWPHLPRWVGAPLYLALGWGAVAILPDILRNGGVADLVLLAAGGLIYSVGAVFYAMRRPNPWPTVFGHHEFFHACTLVAATCHHIAIYLALYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS03</accession>
    <name>U5VS03_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4HBT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00810"/>
    <dbReference type="EnsemblBacteria" id="AGZ38451">
        <property type="protein sequence ID" value="AGZ38451"/>
        <property type="gene ID" value="AFR_00810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.162"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_13_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="49"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="14168" checksum="8688919D4DFD24E6" modified="2014-01-22" version="1">MSTAADGLQKLFGSAGGGALGERMGIDITEATPERVVGTMPVEGNTQPYGLLHGGASCVLAETLGSVGAVLHGATVDRPFAVGVDINATHHKGVRSGVITGVATPVHRGRTAVTYEIVITDEDGDRVCTSRITCLLRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSC1</accession>
    <name>U5VSC1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39893">
        <property type="protein sequence ID" value="AGZ39893"/>
        <property type="gene ID" value="AFR_08020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1637"/>
    <dbReference type="HOGENOM" id="CLU_2930637_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="60" mass="6345" checksum="4B0054496F133546" modified="2014-01-22" version="1">MIVPGGEGMAEGDSVSGDVGDSALREAAPELRRLQGLERIAALGEAGAFDELLDKRNYRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSD7</accession>
    <name>U5VSD7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07450"/>
    <dbReference type="EnsemblBacteria" id="AGZ39779">
        <property type="protein sequence ID" value="AGZ39779"/>
        <property type="gene ID" value="AFR_07450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1521"/>
    <dbReference type="eggNOG" id="ENOG5033AEH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150001_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="92" mass="9641" checksum="6272AA5ECF2D6E5D" modified="2014-01-22" version="1">MSIFSTILVYAVIPLGIIVVVAALSLSGSSRARPARRYRPGRPYDFKPIWFLASPAQVTAPGTQKALPAGVIEDSSGAAVRPGTTGGASDRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT10</accession>
    <name>U5VT10_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative zinc-binding dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_02880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357808.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02880"/>
    <dbReference type="EnsemblBacteria" id="AGZ38865">
        <property type="protein sequence ID" value="AGZ38865"/>
        <property type="gene ID" value="AFR_02880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.579"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_11"/>
    <dbReference type="OrthoDB" id="972769at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="10"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="329" mass="34943" checksum="381C939CE337E792" modified="2014-01-22" version="1">MKAAVVVTPGTISVETVPDPTPGPLDVVVKPAAVGICGTDLHIMEGEFAPAFPIIPGHEFAGEIVEIGAEVTGYAVGDQVAVDPSLYCGHCYYCKRARGNQCENWNAIGVSKSGGAAEFVAAPMANLFKLPSHLAARNAALIEPLSCAVRGFDVLPRNMADNFLIYGSGTMGLMMLELAKRAGAATVSMVDLNPERLETAKRLGCSAAVTSADELEAPRGWDVVIDCTGVAAAIKDGLGRVGRGGTFLQFGVSAYDARVEIEPYEIYRREITITGSMAVLHSFDRAGELMAAGVLDPEIFISHRFPLDRYAEALEQFRAGIGRKIIIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTB0</accession>
    <name>U5VTB0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bifunctional RNase H/acid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08900"/>
    <dbReference type="EnsemblBacteria" id="AGZ40069">
        <property type="protein sequence ID" value="AGZ40069"/>
        <property type="gene ID" value="AFR_08900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1812"/>
    <dbReference type="eggNOG" id="COG0328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035712_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014636">
        <property type="entry name" value="RNaseH/PGlycerate_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036922">
        <property type="entry name" value="RNaseH_PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="370" mass="39501" checksum="B0162EAB00C7AFD3" modified="2014-01-22" version="1">MTELRVVVEADGGSRGNPGPAGYGAVVLEEGTGQVLLERYASLGTTTNNVAEYSGLIAGLRAAAELGATRVDVRMDSKLVVEQMSGRWQIKNPGLRPLAAEAAKLVDGFTTVTFDWIPRERNKLADALANRAMDEAAGKKVPADTPAFESREPKAPKNWAPPSLDTATRLILVRHGETAMTSQGRYSGRGDVPLSEQGEAQAMAAAGRVAGISRDVAAVVSSPLLRCMRTAEFIAAELGNVKVTVLPELIECDFGDWEGLTFAEVRERWPDELDRWLDSTSVAPPGGESFQVVAKRVRGALATLQSAYPSGTVVVVSHVSPIKLILRDALAASDAFLHRLFLDAAGVSTMDMWPDGGIAVRSVNETAHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTC5</accession>
    <name>U5VTC5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MarR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359921.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09745"/>
    <dbReference type="EnsemblBacteria" id="AGZ40238">
        <property type="protein sequence ID" value="AGZ40238"/>
        <property type="gene ID" value="AFR_09745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1987"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_4_2_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="17729" checksum="B1E6AF841EAB2ABD" modified="2014-01-22" version="1">MASAAPERTVRDLTGLLNMAGHALSNRLAAALAEVGLTPRMQCVLVHALEEERTQIQLAALADLDKTTMVGTVDDLEKRGLAERRPSATDRRARIIAVTEAGRVAAEQGQRIVDEVHAEALGALPEQAREEFVAALSRLVESGRLVESGAEPGPKPVRRARLARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTT1</accession>
    <name>U5VTT1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_09640</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09640"/>
    <dbReference type="EnsemblBacteria" id="AGZ40217">
        <property type="protein sequence ID" value="AGZ40217"/>
        <property type="gene ID" value="AFR_09640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1965"/>
    <dbReference type="eggNOG" id="COG0386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029507_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029759">
        <property type="entry name" value="GPX_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="PTHR11592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00460">
        <property type="entry name" value="GLUTATHIONE_PEROXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 5 6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="15071" checksum="B3E4732991526EA2" modified="2014-01-22" version="1">MDRYRGHVVLVVNVASRCGLTPQYAGLETLHEKYADRGFTVLGVPCNQFGGQEPGTAADIEDFCQVNYGVTFPLTEKVDVNGAGRHPLYTALVGDGPDIQWNFEKFLVGPDGAVAGRYGPQTEPEDGEFVAAVEKLLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU60</accession>
    <name>U5VU60_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Deaminase-reductase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358116.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04360"/>
    <dbReference type="EnsemblBacteria" id="AGZ39161">
        <property type="protein sequence ID" value="AGZ39161"/>
        <property type="gene ID" value="AFR_04360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.881"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_1_2_11"/>
    <dbReference type="OrthoDB" id="1920912at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="4"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="21186" checksum="F01F1029CBCA56E9" modified="2014-01-22" version="1">MRNLVYTGFMSLDGIVDSPGGGPGEEHRSAGWVFKDLEFVPEAWTLKGDELADTTALMFGRQSYQAFSSTWPGSEDHADYKDLPKYVVSSTLPEEALVDGWGPTTILRSTEDVAALKKSEGGGIFIHGSAELTRRLADAGLIDQYNLLVFPVLLGAGKSLFDRADRDKHMLTLRESETYKNGILKLIYDVKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU87</accession>
    <name>U5VU87_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10750"/>
    <dbReference type="EnsemblBacteria" id="AGZ40439">
        <property type="protein sequence ID" value="AGZ40439"/>
        <property type="gene ID" value="AFR_10750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2191"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055817_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043917">
        <property type="entry name" value="DUF5753"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19054">
        <property type="entry name" value="DUF5753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="285" mass="31557" checksum="E9677CB28B1BDB6B" modified="2014-01-22" version="1">MTEPVGPALARALLSKELRALRGDAPAADVAGAMHWSLAKLNRIENNKVTIQALEVEALAKHYGVTDAEALERLVGLALISRQRMWWRDRHFPDELTNFIAFENDASHLYAYQATFVPPLLQTRAYAEALTSSVLQKPVDDPTVAGLVDARMKRRETLMARLASGHPPSVSQAIDESVLLRPIGGAAVMDAQLDHLVEMAGRETVELTVLPLRLGVHPGLSGAFELLTFTEDRDLDVVFIETPGTDFLLTDTENTAPFRRVMDELLTMGESLDETVRKVRKTRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU92</accession>
    <name>U5VU92_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_10775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360326.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10775"/>
    <dbReference type="EnsemblBacteria" id="AGZ40444">
        <property type="protein sequence ID" value="AGZ40444"/>
        <property type="gene ID" value="AFR_10775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2196"/>
    <dbReference type="eggNOG" id="COG2321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059329_1_0_11"/>
    <dbReference type="OrthoDB" id="1175086at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007343">
        <property type="entry name" value="Uncharacterised_pept_Zn_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30168">
        <property type="entry name" value="PTHR30168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04228">
        <property type="entry name" value="Zn_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="261"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="262"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="32165" checksum="7059F1C38326188D" modified="2014-01-22" version="1">MELNENARVDTSQIDDQRGSGGGGGGIGGLPIGGGGLTGIIVTVLLALVGGYFGINNLTGDSGGAPQTGDNTSLSQKCSQSDARGQLDCRNVLYVNSIQDYWVEALPANFGEQYKPAKTVLFSNAVRTGCGNADSGVGPFYCPADDRVYIDLTFYKVLADQLGAPGEFAQPYVLAHEYGHHIQDLLGTEAEMRRAQERDPDSANAQSVKLELQADCYAGAWAKNATGTDDGSGQKIFKSITEQDIQEGLDTAAKIGDDALQQRSGGSVNPAEFTHGTSAQRQQWFSKGYSSGSPKDCDTFAAGAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUC8</accession>
    <name>U5VUC8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_04760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358262.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04760"/>
    <dbReference type="EnsemblBacteria" id="AGZ39241">
        <property type="protein sequence ID" value="AGZ39241"/>
        <property type="gene ID" value="AFR_04760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.961"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002554_0_1_11"/>
    <dbReference type="OrthoDB" id="811938at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013587">
        <property type="entry name" value="Nitrate/nitrite_sensing"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08376">
        <property type="entry name" value="NIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="563"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="674"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="827"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="733"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="929"/>
            <end position="956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1083" mass="114801" checksum="405EEFFC27C29151" modified="2014-01-22" version="1">MSTGSSAQASTYPAGGTGLPAGSSGDAGRSSGSGTRERRGRIPRLRDARIRSKLALILFVPLLAVVLLATVRLVDVGGRALDAGQVEDLTRLSTDISDLTQYIHKERMAAAQYLADPGAKADSYSAASAQSDRRIQAYTTDRGELDDPPAAVQDRLRRIDEHLQTIDATRKKITDRDEIAISEAVLRYGVVITDLVGYGEVLSQYAGEGQVADSLRAVSAFSRAKAGTAEQEAVAYAARASGDVSAEQLSVFTATQTSQQEALEGFALVATPQQQSLVDNTVTGDAVNLADGIATRLSRGDPVASLEVTQSFGAVVDLMRWAEQRLEQQTLTLASDESSSVTRQAALEAALVLLTLIIAIALAVVLARSLNLSLRRLREGALAVANRDLPEAVARLRDVRNLGDGGADDIVRQVRDPIRLTNRDEVGQVAQAFNVVHREAVRIAAEQAALRTSVSAMFLNLARRSQSLVDRMIGELDQIERGEEDPKRLAQLFELDHLATRMRRNDENLLVLAGADSSAPRREDALVVDALRAAQSEVELYNRIEFGTVDTDISIMAVAVNDVVRLVAELLDNATRFSPPNTVVVADGRRIRDYVVIQVEDRGLGMSEEQMDSLNRRLAETPDVDVAAFRLMGLAVVSRLANRYGIRVELRANIEGGTVAQVVLPNHIVVLPNSRPLDPPTRGNRQLEPAPASWNDPMPFGRGGAATATLPAIAPEPWGGQRSGSLPTISAPPQRQPELPAHPHLPVQPSQLPGRGDGFPTPDRPALPKRGDSSPADRPPLPTRVTQPVPPGEPAPAYGAGSPTMAYPTMKTAAPDREVQQNLAPSVGFVTPEGGTIPAPRRPASPDPNGWGAALASMPPAPPPPAPARADDRAETSPIFLEMQQSWFKGHDGPISEEWSMPTAGYAPPPNQPQSAAATAAPAAAAPVAAPARPAPPARPAPVSPAVPPVAPISRPPSASPNAGQNGSRPADAGPDSGAQAVPRPRKSAEDAWRTAADDGWQRAMAAAEPTVADTTRSGLPKRVPQAQLVPGGVQSSPRNQNRRSPDEVRGLLSAYHRGVQRGRTAGSEEAAAGAPAPKENEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUJ8</accession>
    <name>U5VUJ8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bleomycin resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11745</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bleomycin resistance protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11745"/>
    <dbReference type="EnsemblBacteria" id="AGZ40638">
        <property type="protein sequence ID" value="AGZ40638"/>
        <property type="gene ID" value="AFR_11745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2392"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121379_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000335">
        <property type="entry name" value="Bleomycin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045517">
        <property type="entry name" value="Glyoxalase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19581">
        <property type="entry name" value="Glyoxalase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20066">
        <property type="entry name" value="Glyoxalase_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="3">
        <location>
            <begin position="63"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="22214" checksum="A5CC1D6CD2607184" modified="2014-01-22" version="1">MVRSVPDPKNMAKALRGELRSRFEVDATHSDCLEIVARQHGVDNWNILASHQSAEPALPWGARIGTIPVLRIFAVDPALQFYVDFLGFTLDFGGPSGGPGTAYYGQISYAATTFHLAEAAYSASQGATVFIWMDGIDQLRERLNHRRTEVPVWGPAVWTPEVEKAQWDGRVLTIADPFGNHLRFSEPDDPKQRAGLPRWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUV5</accession>
    <name>U5VUV5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD(P)H-quinone oxidoreductase chain 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11700"/>
    <dbReference type="EnsemblBacteria" id="AGZ40629">
        <property type="protein sequence ID" value="AGZ40629"/>
        <property type="gene ID" value="AFR_11700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2383"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_616683_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="430" mass="45152" checksum="C6D7C6B65511F7BB" modified="2014-01-22" version="1">MSELQAERPAPAPTRRGDPAGWLRGQIREPFVQLLASQLLTGGVALAANILMVRALNPSGRGEVALMLQVVYLATQVLLLGTERSFVASYHDVAPAVAVRAYAKLMVVPALAGIAAAAVFTSVAPQRFNPGPAIVAMIVAYALVEATSLATRAVAIAVGRVAEFLYARVVESLLLLVLLVVLFTADVAHPVVWIAAYLLSGAVPTFAYVVIWLRLPVRDETPVLPGQNRVVRQEGLALFPAALSNMAMLRVDRLALPALASTSALGLYAAVATMTELLAWPLRAYADSRLGKWRSSHRGEGLDTRPIIVAAAVYALVVAPIAAGALYLLIVPIFGEQYAPAKVVVLPLVAAAGLYAVSRISLGLLIAKGHSALVSAAEITGFVVSFAAYLLLIPAHGILGAAYGSLLGYGACLAFALVTSRITRDRVTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVA4</accession>
    <name>U5VVA4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_06730</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359166.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06730"/>
    <dbReference type="EnsemblBacteria" id="AGZ39635">
        <property type="protein sequence ID" value="AGZ39635"/>
        <property type="gene ID" value="AFR_06730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1375"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015854">
        <property type="entry name" value="ABC_transpr_LolD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220:SF654">
        <property type="entry name" value="PTHR24220:SF654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="12"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="275" mass="29301" checksum="5BF2E8C3D61F35C5" modified="2014-01-22" version="1">MTTDREQVLEGRELLQLYAAPTGPTQALRGVDVTVTGGRISAITGPSGSGKSTLLAVLALRERPAGGELRHRGRLVSSLTRHQLQRLRRREIGWVAQRPTHSLFPQLDARGQIEQMARLRGVRVDADAALAEVELTARATARPAVLSGGEQQRLAVAAAGVGAPALLIADEPTAELDDDSAALVLRLLRSRADQGTAVVLATHDARAVAAADTVLRLRQGVLSGEHDAGQIRTTTIDGLGRLQLPEEALPLFPDGRVIVTVVDGHVELHHPEDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVI5</accession>
    <name>U5VVI5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_06735</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06735"/>
    <dbReference type="EnsemblBacteria" id="AGZ39636">
        <property type="protein sequence ID" value="AGZ39636"/>
        <property type="gene ID" value="AFR_06735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1376"/>
    <dbReference type="HOGENOM" id="CLU_314889_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665253" evidence="3">
        <location>
            <begin position="38"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="798"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="850"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="892"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="928" mass="95456" checksum="ACD4410D76337DAC" modified="2014-01-22" version="1">MAPADRLTVAAVAGGLRQRAWSLLTVSAFLVLAAAGAASPMFAEASGNAAFQARRDQVPVTARQNDAPVVRLSANAGPTSLEQESVLEDLRAVPGLTEPDLTGGSFGAETAGPLFWGSTVSLGAERERARLFAVGSPESDLVAVGKPAGRDGVWLPEPVATALGASAGDRIVLAVVVGKDGEPRQTTVAVDGVYAVDAAGRLPADPPGSRKWALRQGDTPGDTEYRTLPAYLLIGATATVERLAQAVGDEIFWSAEAELVPGSSLAEARQTAEGVEDLRRSYATSVAVEDDVRALRFASGIGRIVDASQATTDQVRQRTRPVEWAAIIVGLASVLAVALLSARRRERELRHVVAVGLSPVRVGGLWGLENLLPALLGAGLGWLVAWQLVTRLGPPGAVTESLRPAALLAGAAALAGLVTVAGVGAAAAARRVRPAPPATVRRALPWAAMVVVIALVAAGGLVSAGGAADGARGVDLLVPLLVLAAVGVVAGRLPGLLAGRRRRTARLPGSPGRAVLWLARRRLGSGGTERRLAVVVVTTGLGMLLFALSAVQSTAVSADDRVAVASGAEAVATLQGSWEVDKGAAQAPPEDPLGAEPPDKPVPGVRNPPMPAGDTVVWRTDTTSPLDDDQRDLLLIDPAQFRDAALWGRGADLAEARAAVADLVATPVDAEGTPRVITVADPASDRLDAIQVEIGFSRANLAVASRVAAFPGLQSRPMYVAAADPTLKKLGRDDPRLRPLRQQSVNAVLVQAQLWSSAGAAGIQAVTGANGVVPERVSTADQLRQDAAYVATGRARGYQLAIAGYLALLAVLTLCIYAQRTAALRGPTDLMLARVGLGRARIRRAQTLEFVLLAAVAFAAAVGGVAALAPLGTRLLDDQPRLLPAFDLQLSGAGVAVTAGVAVLATVLAVALTAVRSATAEEDAYRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVJ5</accession>
    <name>U5VVJ5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_07205</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359261.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07205"/>
    <dbReference type="EnsemblBacteria" id="AGZ39730">
        <property type="protein sequence ID" value="AGZ39730"/>
        <property type="gene ID" value="AFR_07205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1470"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_3_11"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="98"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="164"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="370" mass="40088" checksum="7EE4400BAF27CF52" modified="2014-01-22" version="1">MKLTVRGRLTLVYGGLFLAFGVVMLGVTYVLVSQRVPTKTVMSSVSAPGPARPEPEMIFKRIAEDTRDDALGALLTQGGIALLVVGAIAIALGWLVAGRMLQPLHQITATARRIAESPAADRGLHERIALTGPDDEIKQLADTFDLMLTRLDRSFDGQRRFIANASHELRTPLTLNRTLLEVALQQESASPEVRQLGTTLLAINDRHGRLIDGLLMLARSERELTEHSYVDLADIASHVSSDDVRTELGEAATVGDPVLLERLVQNLVDNAVRHNVPEGGWVRVVTYEKEGLATLEVSNTGQLVPRYEIEALFEPFRRLGTDRIAGPGVGLGLSIVRAVARAHGGEVHAVPREEGGLVVTVTLPAAQAWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW95</accession>
    <name>U5VW95_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359467.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08235"/>
    <dbReference type="EnsemblBacteria" id="AGZ39936">
        <property type="protein sequence ID" value="AGZ39936"/>
        <property type="gene ID" value="AFR_08235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1680"/>
    <dbReference type="eggNOG" id="ENOG5033C8D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142819_0_0_11"/>
    <dbReference type="OrthoDB" id="2042592at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="102" mass="10673" checksum="0DFA7E3DC4F960F7" modified="2014-01-22" version="1">MRGKLMFITGLAAGFVLGSRAGREKYEEISANAKKVWEHPTVQEAAGVAQAQANKLYTEGKDKLNQSKLGEKLHSASTSSSDSTLSSTGASTSSSNGTGTTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWF7</accession>
    <name>U5VWF7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361253.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14560"/>
    <dbReference type="EnsemblBacteria" id="AGZ41194">
        <property type="protein sequence ID" value="AGZ41194"/>
        <property type="gene ID" value="AFR_14560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2957"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051715_3_1_11"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="24"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="30149" checksum="BA882BB8FB1990F2" modified="2014-01-22" version="1">MTDHLTASVDQQVAAANASGKRPVVFIHGLWLLPSSWDRWAAVFAEAGYAPVSPGWPDDPETVEEANAHPEVFAGKSVGDVADHYSEVISRLDRKPAVLGHSFGGLLTMIIAGRGQSAASVAIDSAPFRGVLPLPFSALKASSPVLGNPLNRHRAIPLTYDQFRFAFASAVTEDEAQQLYKEFAVPASGEPLFQAAAANLNPWTEAKADYRNPDRGPLLIIEGIGDNTSPPAINRAAYKLQSDNAGTTEIASLDNRGHSLTIDSGWREVCDTALSFIRRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX07</accession>
    <name>U5VX07_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative LysR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09805</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09805"/>
    <dbReference type="EnsemblBacteria" id="AGZ40250">
        <property type="protein sequence ID" value="AGZ40250"/>
        <property type="gene ID" value="AFR_09805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1999"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="29925" checksum="BCA0C11B3CAA59EF" modified="2014-01-22" version="1">MSKQVHTLEETLGAPLFERVRGAVGLTAAGETLLPLARRMVADADAARDAIQEIVGLRRGRVRVGATPSLCSSLVPTVLRRFRGEHPDIELHVNEGSSQDLIADLLARDLDLALIVQPEHGVDPALHAAPLLRESLVVASVRSGPPPTTHVQLDLNELRDQPLVMFRPGYDLRDVTLEACRRAGFTPHFAVEGGEMDAVLAFVEAGLGVALVPSMVLANRPLLRATPLAPPGMRRTIALAHRRRAILPHAAEALRATLLAHLATGELPIGVETLEQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX54</accession>
    <name>U5VX54_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HNH endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359917.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09735"/>
    <dbReference type="EnsemblBacteria" id="AGZ40236">
        <property type="protein sequence ID" value="AGZ40236"/>
        <property type="gene ID" value="AFR_09735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1985"/>
    <dbReference type="eggNOG" id="COG1403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099824_2_0_11"/>
    <dbReference type="OrthoDB" id="1843957at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029471">
        <property type="entry name" value="HNH_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14279">
        <property type="entry name" value="HNH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNHc" evidence="1">
        <location>
            <begin position="69"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="147" mass="16122" checksum="F6ED86D056500B30" modified="2014-01-22" version="1">MSVVLVLNADFGPLHRVSVRHAIRMLFREVAVVEEAQPDARMGVFPIPTVVRLVSYVVTRWRHSRGPGWSRAGVLTRDGRRCAYCSGAASTIDHVLPRSRGGGNTWLNTVAACGRCNQRKGDRTPAEARMPLRWAPAAPSWAALAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX69</accession>
    <name>U5VX69_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361451.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15550"/>
    <dbReference type="EnsemblBacteria" id="AGZ41392">
        <property type="protein sequence ID" value="AGZ41392"/>
        <property type="gene ID" value="AFR_15550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3158"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_2_11"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="26990" checksum="6ABA09E3079D6FB7" modified="2014-01-22" version="1">METLDLDTATVVYTETGEGHPVLLLHGGGGPQTVAGFAELLAGERPARVITPTHPGFGGTARPDTLATIGDLARLYADLLDRLDLADVTVVGNSIGGWIAAELALLGTRRISGVVLVDAVGLEVPGHPVADFFGLTFPEIAERSYFEPAKFQIDPGALTPQQQTVMAGNRQTLSVYAGTMTDPSLRERLSGITVPVRVIWGDHDRIADTDYGRAYAEAIPGAEFVLLTETGHLPQLESPKRLLPAVWDFVKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXQ4</accession>
    <name>U5VXQ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361761.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16830"/>
    <dbReference type="EnsemblBacteria" id="AGZ41644">
        <property type="protein sequence ID" value="AGZ41644"/>
        <property type="gene ID" value="AFR_16830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3414"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_0_11"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="138"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="348" mass="36811" checksum="D722FACCDB3DA8A0" modified="2014-01-22" version="1">MSTIRRIAVVGAGVIGTHHGLVISQLADRAELVAVADVELGRAERLATERGGRAFTTLSEALAAVDVDIVVVCTPTGRHAEVAIEALAAGKHVIVEKPADITVERTDDIIEARDKAGTLITVISQHRFDPATETTLAAIANGELGRVTSGIASIDWWRGQTYYDSGDWRGTWALDGGGALMNQGVHTVDLLVAALGRPVEVFAYTGTLAHERIEVEDVATGVVRFESGALGVLHATTAAYPGLSARLQVHGDRGSAVIDNDQLSFLHTTAPGAEPDDRLMGVTAQREMPTAGSNPGMLSDAHRLQYLNFLGALDGTEELRVDLETNRRSIAVITGAYESARTGKPVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXQ8</accession>
    <name>U5VXQ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40520">
        <property type="protein sequence ID" value="AGZ40520"/>
        <property type="gene ID" value="AFR_11155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11155"/>
    <dbReference type="HOGENOM" id="CLU_2839797_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="65" mass="7170" checksum="11444650D69F1D31" modified="2014-01-22" version="1">MVRFQVVQVRAVQAWAARGRVALHPAPVAWAHQAQVLQALVVQARAGRVRPVGDRARWGPARAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXT7</accession>
    <name>U5VXT7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361843.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41684">
        <property type="protein sequence ID" value="AGZ41684"/>
        <property type="gene ID" value="AFR_17030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3455"/>
    <dbReference type="HOGENOM" id="CLU_2730840_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="7904" checksum="8EA0F60571A40969" modified="2014-01-22" version="1">MLDRYLPSLPDLFDPTPLVWIVGAFTLYVLFLLPFALLKGEVGKNARAILKDLFALFHALLDVFKGRGGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXX4</accession>
    <name>U5VXX4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative flavohemoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11160"/>
    <dbReference type="EnsemblBacteria" id="AGZ40521">
        <property type="protein sequence ID" value="AGZ40521"/>
        <property type="gene ID" value="AFR_11160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2273"/>
    <dbReference type="eggNOG" id="COG0543">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1017">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026437_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="SSF46458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="1">
        <location>
            <begin position="147"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="375" mass="40323" checksum="708E1464599507A0" modified="2014-01-22" version="1">MDPADDAALHEIQRLLSNSLSLAGGPEDVAVRLRAALAQAQPALFAKLPGGPVWQAEQLAQALTWLVHNLDQPPLLAAASGRLGVALAECGVLPQQLQLAGAALAEAMRAGMPANGWRQDFDQAWRSTWQHAYEWIAHGMAGAAYQPAVWMAVVVGHERRRDDLAVLRLRPYLPMPYRPGQYARIEVPELPGIWRPYSLAGAPRRDNIVELHVRAKSEAGVSGTLVYHTQVGDTVRIGRSEGEMGLPESGRDLLMIAGDTGVVPLKALLTELAATGDPRSAVLFWGVRTLAELYDGDDVAEIARACRRATVIPVISEGEAGPYASGLVTDAVAAYGEWSEHEVYLAGPPLMLVATSAALQLLGVAPERIHHDRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY57</accession>
    <name>U5VY57_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360680.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11755"/>
    <dbReference type="EnsemblBacteria" id="AGZ40640">
        <property type="protein sequence ID" value="AGZ40640"/>
        <property type="gene ID" value="AFR_11755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2394"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_1_11"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="178" mass="20100" checksum="77B5399344BFBE54" modified="2014-01-22" version="1">MTAAASPGRDVVLETDRLLLRPWRVAEAVVQRELWTERDPRVPPHRRIDAEGHPTVAELEDYIRANPPSSSLGLLAAERKADGEVIGYCGLIDGRGSHGEPELAFELLQRVWRQGYATEASSAVLDWARSSGYERLWSTVWDWNTASLRVLAKIGFTETERQEKDPVRGTTLFLTKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYV9</accession>
    <name>U5VYV9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Daunorubicin ABC transporter ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_18470</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362345.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18470"/>
    <dbReference type="EnsemblBacteria" id="AGZ41972">
        <property type="protein sequence ID" value="AGZ41972"/>
        <property type="gene ID" value="AFR_18470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3748"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DUF4162"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DUF4162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="33012" checksum="E996D39E24027D58" modified="2014-01-22" version="1">MSPAAVEAIAIGRKFGRRTVLADVSLQLQKGEILGLLGHNGAGKSTLVKILSTALPPSAGAARVAGFDVLSQAAQVRSRIGLAGQYASVDENLTGRDNLVLLARLCGASRRAARERAGELLDTFALSEVAGRQARTYSGGTRRRLDLAASLVASPAVLFLDEPSTGLDPVSRTALWDLIRSTADQGTAVLLTTQYLEEAERLADRIHLLSDGRTVAAGTVEELKRRVGESAVHVVLADPGTAGTAITCLRRALGREATAAPGEGRLSVPVASSVDVAAVVRALDGAGVTIADISVAKPTLDDVYLSHVRPHHRGSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYW2</accession>
    <name>U5VYW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_13170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360979.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13170"/>
    <dbReference type="EnsemblBacteria" id="AGZ40920">
        <property type="protein sequence ID" value="AGZ40920"/>
        <property type="gene ID" value="AFR_13170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2674"/>
    <dbReference type="eggNOG" id="COG3967">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_6_11"/>
    <dbReference type="OrthoDB" id="1261526at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="27107" checksum="985C4DEDAA2AC55C" modified="2014-01-22" version="1">MNITGNTVFIPGATSGIGLGLALRLHDRGNTVIVGGRRTELLEQIRTQHPGLDTITIDTTDPASITAARDELATRHPGLDTLITMAGIMEPEDLRDPASLDVAERTVAVNLLGPIRLINAFLPGLLARPSATVMTVSSGLAYVPLPATPTYNATKAAIHSFTESLRVQLFKSEVQVIELVPPATRTTLMNQQNSDVAMPLEEYLDEVMSLLEADPKAEQILVERVKWQRNAEAEGRYSDVLGVLSGRYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ20</accession>
    <name>U5VZ20_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362472.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19135"/>
    <dbReference type="EnsemblBacteria" id="AGZ42099">
        <property type="protein sequence ID" value="AGZ42099"/>
        <property type="gene ID" value="AFR_19135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3886"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_8_0_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022290">
        <property type="entry name" value="CHP03858_lucif-like_mOase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03858">
        <property type="entry name" value="LLM_2I7G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="344" mass="37725" checksum="6796B0C68813F6A4" modified="2014-01-22" version="1">MLPVEFGLDTFGDVTAGPDGTTLPYAQVIRHVVEQAVLADEVGVDAFGLGEHHRDDYAVSAPEIVLAAIASRTRRIRLGTAVTVLSSDDPVRVFERFATLDAVSQGRAEITLGRGSFTESFPLFGFDLADYDRLFAEKADLMAALLTEGPVTWQGSVRPPLTDQHVYPKTTSGRIPTWIGVGGSPESVVRAARYGFPLVLAIIGGDPARFAPYVELYHRALDQYEREQLPIAVHAPGFIAATDEEAVETLWPHARAVITRIGAERGWPPLTRDRFETDIAEGAWHVGSPETVARKIARTISVLDVQRFDLKYSAGTLPHEAMMTAIHLYGTEVIPRVRELLENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ80</accession>
    <name>U5VZ80_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PAS/PAC and GAF sensor-containing diguanylate cyclase/phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_20105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20105"/>
    <dbReference type="EnsemblBacteria" id="AGZ42293">
        <property type="protein sequence ID" value="AGZ42293"/>
        <property type="gene ID" value="AFR_20105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4078"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="352"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="494"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="746" mass="79701" checksum="2B519FE8220D36C2" modified="2014-01-22" version="1">MGVQSPPWRGRRLSSPAAFALVVVVLAGQVVAAFDGELRKYILGLSVLLLDALGVVVSLRAGRRGNTVAWQIVSAGRLFSLIGVVTLAASVATLSHGWWWIGVVSRLLMFVLLAAAALVAPAQRLSGRQRYAFAAEAVTVLGAGFMVVWYFVINPAIDGHPPQDLWVAEIGYPIGDLLLLTGAAVVVLRGTISRLSSPLTMYVGGLVCYLVGDAMWSALGVHGVEASESPFAGFFLVIASLLITLSAMLYQPMAPTRSRSRTARASAWSTHLPTFAVAVGGLLLLVVTVRENDMLPWGGLVLGLIVMASAMAARQMFSLRDTRDLVVSDLLTGLANRTGLDNAITRAVKRREHVAVLLIDLDGFKLVNDAYGHAAGDTVLVEFAHHLRSTVRTSDVPARIGGDEFAVLLTEIVTPEHATAAAQRILAAVAANPVRLGEDVLPIRASIGVAIGTGEDSTKDLLRRADVAMYQAKRAGTHAWVLHDPSMIDRRAEDAALSDDLAAALDRGELNVLFQPMVDLVSGRPIGAEALVRWQHPTRGVVSPVRFIPIAERSGIITEIGLFVLEQALLQLKAWRQPLYISVNLSPRQLSEPTIVHDILAVLGRTGMPPESLVLEVTESAIVDENTGIAALRALREHGIRVAIDDFGTGYSSLQYLTRLPVDILKIDRSFVGELNGTPAGSAITEAVIRLSQVLNLTTVAEGIETAEQAAELLTLGCDTGQGYLYARPLPGPDLDDLMTAVRADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZV1</accession>
    <name>U5VZV1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inosose dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362786.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20710"/>
    <dbReference type="EnsemblBacteria" id="AGZ42414">
        <property type="protein sequence ID" value="AGZ42414"/>
        <property type="gene ID" value="AFR_20710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4199"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059523_0_1_11"/>
    <dbReference type="OrthoDB" id="1194699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="33"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="33128" checksum="E3B36742BC128E58" modified="2014-01-22" version="1">MAEIRHLQLGSCPDSWGVWFADDPRQTPWPRFLDELAGSGYRWLELGPYGYLPTDPAELRDELAKRDLSCAGGTVAGVGGPHKDFATVLAETRKVAELTAALGAENLIFVPVPGYRDDVTNAYHEPAELSPDGWQALIDNSNTLGRVLAEEYGLKMRFHPHADYHVETQAQTERFLAETDPRYVSLCLDTGHLAYRQADVPAIIRKFPDRIGYVHIKQMDPAIAERAVREDMAFGRAVAMGASVEPPAGLPDIPSVLEALNELDAELFVVVEQDMYPVDFDLPLPIATRTRQYLNSVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZY5</accession>
    <name>U5VZY5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kelch repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15015"/>
    <dbReference type="EnsemblBacteria" id="AGZ41285">
        <property type="protein sequence ID" value="AGZ41285"/>
        <property type="gene ID" value="AFR_15015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3049"/>
    <dbReference type="eggNOG" id="COG3055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004253_10_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="SSF50965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665933" evidence="1">
        <location>
            <begin position="25"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="347" mass="36965" checksum="5DB351C24C5F15ED" modified="2014-01-22" version="1">MPTRNSALWAAVLMAGSVAGPAFAAPAQSRTAQQQATWNVEVPSPESRSEVGVAALNGRVYVVGGGVERAGAEPQFASDLVTSYDPRTGRWANHAPLPRELSHVGVAALNGKLYAFGGFTNVVHIDPQPLAYEFDPRRERWTRLPDLPARLGSVSVAAVDGKLHLIGGRDSRRIETIPGVPFELGFGTVRSHFVFDPGRRTWSTAPQLPAESRDHAGIAVLGHRLHVIGGRVEDVDQNLARHDVYDTRSRRWTRAATLPVPRSAGAVVVLGGRIVYAGGECGPGGETFDDVTVYNPRTDRWSTVASMPLGGRHGLGAAQVAGRAYYIAGAPTCGGGSSVDTLELELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W091</accession>
    <name>U5W091_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycoside hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_15590</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15590"/>
    <dbReference type="EnsemblBacteria" id="AGZ41400">
        <property type="protein sequence ID" value="AGZ41400"/>
        <property type="gene ID" value="AFR_15590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3166"/>
    <dbReference type="eggNOG" id="COG3507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029926_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665943" evidence="5">
        <location>
            <begin position="17"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="3">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="484" mass="52242" checksum="2C9337A543C7500A" modified="2014-01-22" version="1">MCVALAAFSLPGAAAAAIPDYTTAEAGNPFVDGLYADPDVAVYGSTYWVYPTSSRSYAEQTYLDAFSSQDLVHWTKHPNVLTTARVSWAKYAMWAPAPVQRNGKYYLYFAANDIQNNSALGGIGVAVADRPEGPYTDAIGRPLISQFVNGAQPIDQDVFIDDDGQAYMYYGGWGHANVVKLNSDMTSLGTFADGTTYKEITPSNYTEGPQMFKRNGKYYLMWSEGGWTGPNYSVSYAIADSPTGPFVKLDRVLDQDPAVARGAGHNSVINVPGTSTWYIVYHRRPLSQTDGNYRQLAYDRMYFNADGTIRRVAMLVKDNFDDTNAVGWRTYGGGWSTSGGQYLAASSAGGKSLLDVNFANLAYDADITLTGGGGDGGLLFRTTGAAVGTDSHQGYYAGISTQGRVVLGRMNNNWTQLASAALPITQGTRYHLRIEAIGSSIKVYVGDLTTPKISVTDTTWTSGATGVRVFQTAASYDNIAVTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0L3</accession>
    <name>U5W0L3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_15970</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361535.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15970"/>
    <dbReference type="EnsemblBacteria" id="AGZ41476">
        <property type="protein sequence ID" value="AGZ41476"/>
        <property type="gene ID" value="AFR_15970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3241"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="30"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="62"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="342" mass="37213" checksum="F65777EF86F2A386" modified="2014-01-22" version="1">MSELGHEPDDDGVIVLSGLTKLYEPTPRWMRMFARSHLTEPVKALDGIDLVVRPGEICAVVGPNGAGKTTLFRIIIGLTEASSGQGRVLGLDVARQSEQIRQVVGWMPAEDRSLLMRATVRENLQLHGRLQGMSRTELQERIPYVLQTVDLTAKTDVVIASLSAGMKARVRLARALLPGPRALILDEPTGAVDPIAAHDLLKLIMDLVKQEQLAVLISSHRLEEIEALQSKALLMDGGRIKYSGDLDSLRQKWDRPQLELVMADESATRLVADGLQAQGLDVVVDGFAVRCRLGDTLTIGAVLGRLGASAASIQHIREIPMPLRDLIAQIYRLDPAADGGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0T6</accession>
    <name>U5W0T6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GyrI-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363128.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22395"/>
    <dbReference type="EnsemblBacteria" id="AGZ42749">
        <property type="protein sequence ID" value="AGZ42749"/>
        <property type="gene ID" value="AFR_22395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4540"/>
    <dbReference type="eggNOG" id="COG4832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083625_0_0_11"/>
    <dbReference type="OrthoDB" id="1748122at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029442">
        <property type="entry name" value="GyrI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008319">
        <property type="entry name" value="UCP031644"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06445">
        <property type="entry name" value="GyrI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031644">
        <property type="entry name" value="UCP031644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GyrI-like" evidence="1">
        <location>
            <begin position="20"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="22482" checksum="41CE67773425AC7F" modified="2014-01-22" version="1">MAPKTDFKKTLDSYAATRGKFRVVEVPERQYLMVDGRGDPNTSPEFGAAIEALYPVAYKVKFASKNELGRDYVVPPLEGLWWADDMDAFTDRRDKAQWEWTLLLMVPDWIDSSMVDAAVAKAGPKQDVRLETLSEGRCVQTLHVGSFDEEADVLRRMHDEVIPAQGLTMAGKHHEIYLSDFRRVEPAKLRTILRQPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W172</accession>
    <name>U5W172_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid adenylation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_23675</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23675"/>
    <dbReference type="EnsemblBacteria" id="AGZ43003">
        <property type="protein sequence ID" value="AGZ43003"/>
        <property type="gene ID" value="AFR_23675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4797"/>
    <dbReference type="eggNOG" id="COG1020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensatn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010060">
        <property type="entry name" value="NRPS_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01720">
        <property type="entry name" value="NRPS-para261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="915"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="1915"/>
            <end position="1990"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="2908"/>
            <end position="2982"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="950"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="1950"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="2943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="3458" mass="371681" checksum="A3066FD164BCB66B" modified="2014-01-22" version="1">MTAPAGLADILPLTPLQEGLLFHAHLAADGPDVYTGQLTIALHGPLDAERLRAAGQALLDRWPNLRVAFRRRKNGQAVAMVPKHVVLPWATLDLTPEELPAFLDADLRQRFDPASAPLLRMTLVRLGPGDHRLVFTHHHLLIDGWSIPLALDELIAHYRGDTLPEPPAFKAYLGWCAAQDRPAAEAAWRAELAGLDGPTLLAPQPPARAVVPDSAVRDLSPELTARLVALGRSRGLTLNTLVQGAWGIVAGALTGRNDVVVGASVAGRPPDLPGSDRMIGLFINTIPVRIKLDPGATVVAALRRLQDGQVRVMDHQYLGLSEIQRLGGHGELFDTLTIFENYPLGDSLPASGDGLRVTGAEVRDCAHYPLTLTARPGERMHLDLEYRGDLFDPAAAALLLARLERVLTRICDAPDAALATTDVLSPAERQTILRGWNDTDAPVPAGTITGLLDEQVRRTPDAAALVASGRQWSYTELQEWSHGLAGVLHEHGVGPGDLVALALPRALTVPALFGVLASGAAYLPLDLEQPADRIAGMLDDAAPALLITVGGVDVPWTGARITVGPDTPDGMPFTRAIDPESPAYVIYTSGSTGRPKGVVVPHRGIVNLFAGHQKRLMPGPVRRVAHVASFTFDGSWEPLLWLLGGHTLHVVDADVYRDARALVGYLSEQHVEVLDVTPTYLRELVPAGVLEAGLRVLLVGGEAIDPQLWQQICAVEGLVCHDLYGPTEASVDAYGWHGPDRSAYRLDNVRTYVLDNALRPVPPGVLGELHVAGAGLAHGYLNRPGLTAGRFLADPFGPAGSRMYRTGDLARWTPDGVLEFAGRADGQVKVRGFRIELGEIEAALGQAAVLVRDGRLVAYVVGAPVDVDALRAKLPDYMIPAVFVEVDALPRTVAGKLDVAALPDPDLSLLSTGRVARTTREQQLCDLFGEVLGLPAVGIDDDFFALGGHSLLVMRLAGRVRSVLGVELPVRAVFDAPTVAALLPRLEGSNRAPLVATERPERVPLSYAQQRLWFLYRLEGPNPTYNLPMAWRLRGRLDRDALIAAVGDVVARHETLRTVFAEDTEPYQRVLPAGPLTIPVEDIAEADVADRLAALGAYAFRLDQEPPLQARLLRVAPDEHVLVLLLHHIAGDEWSDVPLRRDIVAAYTSRCAGLAPSFPPLPVQYADFALWQRAEADSLAGQIDGWREALAGLPEELELPADRPRPAEATYHGDVVEFGLPADVTAQLRSLARSTGTSMFMVVQAAVAVLLTRLGAGTDIPLGSPVAGRPDSALDDLVGFFVNTLVLRTDTSGDPTFRELLARVRETDLAAFDRQEVPFEHLVEALNPVRSLGRHPLFQTMVSYLGEADSTWHLGPLRVEAERVRQHVAMFDLSFDFFETRDGVRGELEYATDRYDRNSAETLVERLRRVLTAVAADPSAAISRTDVLAPHEREQPVVTLPVPDGSIVDLFAAQVAATPQATALVSGGREWTFAELDDWSARLAGVLTARCAGPGDLIALATARPLTVPAILGVLRTGAAYLPLDTFQPAGRLATVLEDARPRLLVTTEGADVPWTGARVTVGLEAPAAAPLTPVSVDPQAPAYVIFTSGSTGRPKGVVVPHRGIVNLFAGHRQRLMPGPVRRVAHVASFTFDGSWEPLIWLLDGHALHVLDDDEYRDAGVLVSRLRDQRIDVLDVTPTYLRELIPAGVLEAGLRVLLVGGEAIDSQLWHQICAVDGLVCHDLYGPTEASVDAYGWHGPDRSAYRLANVRTYLLDQTLQPVPRGVLGELYVAGPGLAHGYLNRLGQTATRFVADPYGEPGSRMYSTGDLARWTPDGVLEFAGRADGQVKVRGFRIELGEIEAALGQAAVIVRDGRLIAYVVGGPVDVDALRAKLPDYMIPAAFVEVDALPRTVAGKLDVAALPDPDLSLLSTGRAARNTREQQLCDLFAEVLGLPTVSIDDNFFALGGHSLLVMRLAGRVRAVLDVELPVRAVFDAPTVAGLLPRLESARRAPLVAADRPARVPLSFAQQRLWFLYRLEGPNPTYNIPMAWRLRGHLDRDALVAAVHDVAARHETLRTVFPDVDGTPYQRILPPEAVPVLFEEGDPGYCFDLDREAPLRVTVTTTGPDEHVLLLLLHHIAGDEWSDEPLRADLSTAYEARRAGRAPDFPPLPVQYADFALWQRAEADGLAGQIAAWREALAGLPDELELPADRPRPAEATYHGDVVEFAVPGTVSRELRHLARTTGTSLFMVVQAAVAVLLTRLGAGTDIPLGSPVAGRPDSALDDLVGFFVNTLVLRTDTSGDPTFRELLARVRETDLAAFDRQEVPFEHLVEALNPVRSLGRHPLFQTMVSYLPGSGDRWQLGDLTGHPEPAGHPSAMFDLSFDFADTPDGTGIEGVLEFATDRYDRDTARTLVDRLQRVLAAVADDASVVVSRVDVLAPDERDRLLSGDNGPAPAGSIVDLWAAQAGSPGIALVSGGRSWTFAELDDWSTRLAAVLAGRGVGRGDLVALAVPRPLTVPAILGVLKSGAAYLPLDTYQPADRIAAILADAAPALVLTTGGTALPPGVARLPIDEPGLAGEPVAEPARPGAEDPAYVIYTSGSTGRPKGVVVPHRGIVNLFASHRERLMPGPARRVAHVASFTFDGSWEPLLWLFGGHTLHVLDDDEYRDDAALVAYVRRHHVDVLDVTPTYLRELIPAGVLEAGLRVLLVGGEAIDSQLWHQICAVEGLVCHDLYGPTEASVDAYGWHGPDRSPYRLANVRTYLLDEALQPVPSGVLGELYIAGPGLAHGYLNRLTQTAERFVADPYGKPGSRMYRTGDLARWNAAGGLEFAGRADGQVKVRGFRIELGEIEAALGQAAVIVRDGRLIAYVVGGPVDVDALRAKLPDYMIPAAFVEVDALPRTVAGKLDVAALPDPDFSALSGGRAARTAREQQLCDLFAEVLGLPTVGIDDDFFTLGGDSIVSIQLVSRARAAGLGISPRDVFRHKTPAGLALTVQAAEPVVTEDPREAWGSAPLTPVMRWLHEVNGPTRGYSQSMLLHAPDGLTATGLADVLRAVTDRHDLLRARITADGVEIPEPGTPLDVVREATYDEATLPTVAAAARDRLDPAAGVLLQAVLLHPGHVLLVVHHLAVDGVSWRILLPDLAAAWADREAGRPIVLPPAGTSFRRWARELERLAAAPGTTAQLPYWTEVLDGPPATLGTRELDPARDTVATCAEQALVLPPEETGPLLTTVPASFHASVNDVLLTGLALALRDWSGGTGALVMLEGHGREEHLVAGADLSRTVGWFTSEYPVRLEVSTENPGDALKAVKERLRAVPDNGVGYGLLRYLHPESAPVLAVRGKPLIGFNYLGRFAAGDAAEAAWEPAGEGWGGGADDGMPADYPLEINATAEDHPDGTRLTATFTWPRDLLTEAQVDELAGRWRAALRALAAHAADPDAGGHTPSDLTLVELSQDDIDSFEAEFADLESEWETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1B9</accession>
    <name>U5W1B9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363499.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23345"/>
    <dbReference type="EnsemblBacteria" id="AGZ42939">
        <property type="protein sequence ID" value="AGZ42939"/>
        <property type="gene ID" value="AFR_23345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4731"/>
    <dbReference type="eggNOG" id="ENOG5032Z2P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190785_0_0_11"/>
    <dbReference type="OrthoDB" id="2032501at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="74" mass="7586" checksum="A67205596043326B" modified="2014-01-22" version="1">MAVSANLDKVLDKAYESSTPSELVNAPVAALAGVSDSDAELLQKAFNIKTIGDLAGNKYFRTASAIADLAAISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1P0</accession>
    <name>U5W1P0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363504.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23360"/>
    <dbReference type="EnsemblBacteria" id="AGZ42942">
        <property type="protein sequence ID" value="AGZ42942"/>
        <property type="gene ID" value="AFR_23360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4734"/>
    <dbReference type="eggNOG" id="ENOG5033MCS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_388643_0_0_11"/>
    <dbReference type="OrthoDB" id="435186at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="710" mass="76217" checksum="66B55CC348270819" modified="2014-01-22" version="1">MLDLLDKLLTFFPVVAMAALGAVTAVKGWLSHRQHSPGVSVEIGAELPVEFEGPTSAERRDALVEAILEALGRTDDEEPGRIVSTGLARPELPLQPSSRDRCLEPGRAYLFWFAIEATPVEGALDDVPRALRLPDEAVAGDAITVALFDYPGELILTPGTNVGTLTLGSDGRITPGRPPTVGLAAPDRLLFPIRTPEEPGIHRLRCNVYCRGTLLQSRVLSVTVEAGAAEREDALQVVVDYAVDTDFEPAALAQIQPLQLSVFANNNGRGTHSFRFFGGEALTATVSVSEHQIQDHIDRARRTLTTVAWGAPEYRDGAGFKYGRPRTARSFSDDLIQLAISGHRLWAHVAGRVADGAAPALPDVSRSRIKELQDLMRRPGMVEFANKVDASFTVPAGLFYDYDLDTTVPVREWRVCPEALDAIRTGADLSRQPCFLGAVEHAETVVCPSGFWGFRHGVTLPQASAFSVGDAAGVAPSADVEATHFIDAEERPRMILGVADDFTDLGGHVAWVEALGGAGFVHRCTTAGRLLEAMRDTALEPHLVYLFCHGSETNGIPALVVGPRKSLGIQYEQIATGNVYWRRSRPLVVLNGCGTTAVQPRHAMNFVDAFIRTAKASGVVGTEITTDARLGARFGQQFLDAFVTGRRTIAESLLSARLSLLAEGDPLGLAFVAYAAPQLRLRARADPCTCGSGRAYGDCHAPVPAASGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W235</accession>
    <name>U5W235_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative FAD-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363883.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25135"/>
    <dbReference type="EnsemblBacteria" id="AGZ43293">
        <property type="protein sequence ID" value="AGZ43293"/>
        <property type="gene ID" value="AFR_25135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5093"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003896_4_2_11"/>
    <dbReference type="OrthoDB" id="482388at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003885">
        <property type="term" value="F:D-arabinono-1,4-lactone oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007173">
        <property type="entry name" value="ALO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010031">
        <property type="entry name" value="FAD_lactone_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43762">
        <property type="entry name" value="PTHR43762"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04030">
        <property type="entry name" value="ALO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000136">
        <property type="entry name" value="LGO_GLO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="413" mass="45084" checksum="5A4F8B7946716479" modified="2014-01-22" version="1">MSDVVKNWAGNITFNASRIHRMLTVPELQDLVAGGGKVRVLGSGHSFNRMADTTGDLVDLIGLPRVVEISADRRTVRVDGGIRYGELADRLHAEGLAVHNMASLPHISVAGAVTTATHGSGVRNRNLATSVAGLELIRGDGKLVTLRRGDEGFDGAVVGLGALGIVTALTLDVVPAFEIRQYVYDDLPRSVLDDNLPEILASGYSVSLFTNWQSTDINHAWLKRTEPMPDGPFFGARPADGPRHPVPGVDATNSTEQFGVPGPWHARLPHFRMEFSPSTGAELQSEWMVPLDRALEAIDAVAAIREQLAPVLQVNEIRTVAADDLWLSMNYHRDSLGLHFTWIADTDRVLPVVAALEEQLAPLEARPHWGKVFTQDPATIRSRYERFADFQALAAEYDPAGTFRNAWLDDILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2L0</accession>
    <name>U5W2L0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364201.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26020"/>
    <dbReference type="EnsemblBacteria" id="AGZ43468">
        <property type="protein sequence ID" value="AGZ43468"/>
        <property type="gene ID" value="AFR_26020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5275"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="190"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="396" mass="42404" checksum="4D8BD11C7308C658" modified="2014-01-22" version="1">MAQIGVLQMRQWRLTAWHPLLVDVLVALVLAAAVLKSTVFRSGEPVTARDMVVVGVAFLAVLLRRSHSKIALGVATIASTVAVVKGVSNPGLIVVLGVITYSIAAHTDRRSSSWFCALAAATVVYGAAVVVSGGTWWRPDVLGVFAWIFMAAAVGDAARTHRAYVAEVEERARRAEQTREEEAHRRVIDERLRIARELHDVVAHHIAVISVQAGAATHVLERRPDQVGPVLAHIRDASETVLREIQSVVGVLRNSDDVPTTEPAPGLARLPDLVGALQAAGFSVRHQRRGAPRELPALVDLAAYRIAQEALTNAHRYGDGRATLLVEYAPSGVILEVTNEIRDGDAVAGTGYGLIGMRERATAAGGTVTADPLGDGRFRVHAVLPTEEATSKGCYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W363</accession>
    <name>U5W363_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364553.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26935"/>
    <dbReference type="EnsemblBacteria" id="AGZ43648">
        <property type="protein sequence ID" value="AGZ43648"/>
        <property type="gene ID" value="AFR_26935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5458"/>
    <dbReference type="eggNOG" id="ENOG5031T4I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_627950_0_0_11"/>
    <dbReference type="OrthoDB" id="1992898at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="436" mass="46392" checksum="6701F5AB781DCA43" modified="2014-01-22" version="1">MTDTDIRTALERATGDLSSPPDLLERVRAGGHRRVVRRRAVLGAGLATVVAGSAAGVGLSRDHGDRRAPVASPLLDGPTRGDLREDTAYLDSVRAAWRDHVGSKRLVGEPKVVWAGTAPHDSRAAVVAQRVREEVASPIGQIDYGMIGFVEQTPAGPVVISLEQMLTLASNSAVALLGPDRDVVMILDDGRDARYSPSLTYDAGGRIRRTFTAPDFRAHDGVFFATAGKQNTSVQIAVRAGRRDGAGSLAVGLANQSALIEAAGGGRPGSGWSGSERLDRRLPGRDRAWPADADLDRDGLGPWDLSTREGYADTYGYWITPAVDTWLIRGATADRRRFVVQTLTAPDGRSRVFLQLETPDAVLNGFTDPGAALTLQVRLPDRQGVVVAAGNATLRYRTRAGGWLPVTGDAALLPDAAREVEVTRAGARPVLVPLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W370</accession>
    <name>U5W370_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20865"/>
    <dbReference type="EnsemblBacteria" id="AGZ42445">
        <property type="protein sequence ID" value="AGZ42445"/>
        <property type="gene ID" value="AFR_20865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4230"/>
    <dbReference type="eggNOG" id="COG3411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119509_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="113" mass="12085" checksum="4B6B450EC921D8FF" modified="2014-01-22" version="1">MMTAGCTITVCRDCCCGNARKHPGVDHDGQLVQLRESLPAPHRVRVSECLDECAEANVIVVHPTPAARLAGARAVWFGLVNTDVTEDITAWVQDGGPGVAPMPAILELSVLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3C1</accession>
    <name>U5W3C1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage II sporulation protein E (SpoIIE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362867.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21115"/>
    <dbReference type="EnsemblBacteria" id="AGZ42495">
        <property type="protein sequence ID" value="AGZ42495"/>
        <property type="gene ID" value="AFR_21115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4280"/>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_43_8_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="312"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="528" mass="56962" checksum="7D1AD991C990D089" modified="2014-01-22" version="1">MSDPFEAAGTLRDAYRSIDWTATSLGPVESWGPELLAALDLTLHTRAPVTLFWGADYTMLYNEAYVPMIGDKHPAALGSTTTEVFAEIWDTIGPMLDSVFAGRGATWVEDLRLLMNRSGFLEETYFTFSYSAVHATTGRIAGVIDIAAETTAQVLGRRRLALLARLNDKLADAEDVRQLLDRALPVLRSATEDLPGVDILLPEMQAPTVAWPAELDRDVVVETTPEGRVARIRLTGAARARSDAMLVSRISPYLAVDDAYLDFFRLLGAALAQGMNRARTRQAERRAAAMERELSEALQRSLLPAPDQPAHIEVAVRYQPAAEGVQIGGDWYDSFTLPDGSLTLVVGDVTGHDREAAVGMSQLRNLLRGISYAVGKPPAEVLGVLGEAMNGFGVDVFATALLAQVEPDTGRTRTLRWSNAGHPPPVFVSPDGSVHLLDTHPETLLGTRGRSIRTNHTVELPPGAAVVLYTDGLIERRGSSLDEGLSELVAALADAGGLTAEQLCDRLLDRFAGAAEDDVVLAVVRAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3F1</accession>
    <name>U5W3F1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Antibiotic transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21265"/>
    <dbReference type="EnsemblBacteria" id="AGZ42525">
        <property type="protein sequence ID" value="AGZ42525"/>
        <property type="gene ID" value="AFR_21265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4313"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="31074" checksum="A16E648EE984839A" modified="2014-01-22" version="1">MVKRFGAVTAVDGISLRIRPGEVVALLGPNGAGKTSTVDMLLGLSDPSAGTARVYGRPPQEAVALGLVSAVMQTGGLLKDYTVEETVKLTAVLFGRGRSEVEESLERAGIADIRKRLVGKCSGGQQQRLRFAMALLPDPELLILDEPTTGMDVGGRHDFWTSIREDASSGRTVIFATHYLEEADAYADRVVFVRRGRIVADGTAAEVKALASGRTVRATLPGFDEARVKELAGVESVEVRGDTVYVHARDTDLVARYLLNETAARDLEITSRNLEQAFLTLTADDGETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W448</accession>
    <name>U5W448_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27680"/>
    <dbReference type="EnsemblBacteria" id="AGZ43797">
        <property type="protein sequence ID" value="AGZ43797"/>
        <property type="gene ID" value="AFR_27680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5610"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057459_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="353" mass="36186" checksum="A3700872C4A9652E" modified="2014-01-22" version="1">MLVLAGLVVAGLRFLPERKPTGTEEATGPPAATAVVTRTDLSTTRTVSGELGYGTPQELEGGAGTVTWLPKQGAVVKRGQPVYRRDDRPVPLFYGRTPLFRPLDRAGLVGRDVRVVVDNLRALGYRTGDQPLPGSPAEPAPLKVHAGDGVMTRSLIAAVKRWQTDHGVPATGVIAPGDVLVHSGKLRVAALIARPGRPSDAPLMAVTGTAKVVTVQLDAGDSGSVRRGDAVRVTLPDGDVAKAKVTSVGTTARAPEGGDGTEQPRTTVTVKLDDSGAVSRLTAAPVQVEVTGETRPDVLAVPVGALLALLEGGYAVQIAGGALVPVDTGLFADGRVEVSGPGLDEGVTVVTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W455</accession>
    <name>U5W455_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_28265</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560251.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28265"/>
    <dbReference type="EnsemblBacteria" id="AGZ43914">
        <property type="protein sequence ID" value="AGZ43914"/>
        <property type="gene ID" value="AFR_28265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5729"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="18"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="108"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="172" mass="18999" checksum="EA08540B13C32051" modified="2014-01-22" version="1">MRGDSRTGDEPYVGYVTARLPALQRAAFLLCGGDAHQADDVVQATITRLYQHWKRVSRSDNVDAYVHRMLVHAVIDSKRHSWSRVQLVGRPPEATGHDPHAGVDERGSLVAALRSLPPRQRAVLVMRFLLDRPIDEVAEALDCSVGTVKSQSSRGLDALRSMLATIEERGHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4A9</accession>
    <name>U5W4A9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560311.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28565"/>
    <dbReference type="EnsemblBacteria" id="AGZ43974">
        <property type="protein sequence ID" value="AGZ43974"/>
        <property type="gene ID" value="AFR_28565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5791"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107911_0_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="23276" checksum="9D832922E0E520C9" modified="2014-01-22" version="1">MNGRTYDNTRRAEQARLTRRRILDAARELIVARGPTSVTMREVAVHAAVSAETVQKTFRTKAALLKETYDVTLAGDDEPVPMVDRPEIQAILAATTPREKLARYATVARVLGDRTGPLVSKLLAGSRSGDPDLADFRETVNRERLIGVTGIVGHLADTGGLRAGLSPEQARDIVWALISPELYDLLITDRGWTPDQYEQWLARTLTDALTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4G9</accession>
    <name>U5W4G9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative cation efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_22755</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22755"/>
    <dbReference type="EnsemblBacteria" id="AGZ42821">
        <property type="protein sequence ID" value="AGZ42821"/>
        <property type="gene ID" value="AFR_22755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4612"/>
    <dbReference type="eggNOG" id="COG0053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="8">
        <location>
            <begin position="232"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="32687" checksum="FC42D001D7106780" modified="2014-01-22" version="1">MSHRLRHALTPHSHDSAGKVDTALESSRAGLRALWISLAVLGATAAVQAVVVASSGSVALLGDTLHNVADALTAVPLGVAFLLGRRAATRRYTYGFGRAEDLAGIVIVLTIAASAVFSGYEAVRRLLDPQDMTHIGYVALAGVAGFAGNELVARYRISVGRRIGSAALVADGLHARTDGFTSLAVLLGAGGAALGWRWADPVVGLLITVAIVLVLKDAAREIYRRLMDAVDPALVDRAETCLRDTPGVLGVGTVRLRWIGHRLHAETEITVGADLSLAAAHAIAVDAEHRLSHAVPRLTTALIHPDPEHAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4J2</accession>
    <name>U5W4J2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anti-sigma F factor antagonist</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28355"/>
    <dbReference type="EnsemblBacteria" id="AGZ43932">
        <property type="protein sequence ID" value="AGZ43932"/>
        <property type="gene ID" value="AFR_28355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5749"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2068071_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12867" checksum="72CF22BCC12EFDBF" modified="2014-01-22" version="1">MILCGDLDGLNVEDLQASLSDVLRRYRPASVEMDAHLVTYLDSGGIRALLRCRAAAQRAGCDLTLIEVSKPVYQVLEITALLDQFGVVEQQSLRLRPRDHSLGLGTGIAGSGERTTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4J7</accession>
    <name>U5W4J7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22930"/>
    <dbReference type="EnsemblBacteria" id="AGZ42856">
        <property type="protein sequence ID" value="AGZ42856"/>
        <property type="gene ID" value="AFR_22930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4647"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088923_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="28489" checksum="7623416966BEACE4" modified="2014-01-22" version="1">MVLLHGFGLDHRSLLPLEPVFERSGRWRRIYLDMPGATGSPANGLGSTQEVAEAVAGEIRARVGDEPFAVLGNSFGGMIARYVAHELRSQVLGLATVAGVFVARHDRRTVPARTVLRREPGIVPLLGAELDEYRADAVVESVDDARVFLEYVLPGVRAADQPALERIAERYSLDREPEDAHPEPFTQPALHITGRQDDVVGYSDAWSRDGHYPRASFAVLDAAGHNLLNEQRDLCAALITDWLRRIVSAVPEDLRPSGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W548</accession>
    <name>U5W548_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24135"/>
    <dbReference type="EnsemblBacteria" id="AGZ43095">
        <property type="protein sequence ID" value="AGZ43095"/>
        <property type="gene ID" value="AFR_24135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4889"/>
    <dbReference type="eggNOG" id="ENOG50332K7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080168_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043231">
        <property type="term" value="C:intracellular membrane-bounded organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="23548" checksum="2D68BC17DB781B68" modified="2014-01-22" version="1">MLADQFYLIAHEDRTGRSRLHPRATGIGLAASLLGELVLDNRLRIFAGDLEIVSNQPPRDALTHSILDLLIAQPQHRDVRTWLAYLSQDAAMRVGERLERSGAVESVTRRRMLSTQTFYMPNNELQRNAAAWAPMRLANLLVRGLDMALSDRVLAGLIAATGLTRHVLWDFEAHRSALTVLPNTVASLPEDLRQLIENTEASVGSVLAVGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W597</accession>
    <name>U5W597_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_24065</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363671.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24065"/>
    <dbReference type="EnsemblBacteria" id="AGZ43081">
        <property type="protein sequence ID" value="AGZ43081"/>
        <property type="gene ID" value="AFR_24065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4875"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_3_11"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="91"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="152"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="39664" checksum="8E7AB4C1A4FA9416" modified="2014-01-22" version="1">MRVNRLRWRLTLLYCAATVVSGVVLLLMIVVLSRNLPPADVRAPFPSRPDIMPPAVPAEFREFRSDVLILPGLALAIMAVLSLGVGWVIAGRVLRPVRTMTTRLQQISGRNVHERLAVPGPRDEFKDLADTVDGLLGRLETALDGHKRFVANAAHELRTPLTLEHALLEEPLIDPDATLEAYRENFERLIVVSRERGRLLESLLTLAGSEHGRIRAELVDLADLAGQALRDRTAELDRRGLRSRTMIGPARLQGDPLLITRLVANLCDNAVHYNVPGGIVEVTTRRDAGRALLVVSNTGPVVPSAEVDRLFEPFQRMRRVADDNHPGLGLSIVRAIAVAHDAALTAAANPGGGLTVEIAFPAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5M9</accession>
    <name>U5W5M9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_30260</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylyl cyclase class-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30260"/>
    <dbReference type="EnsemblBacteria" id="AGZ44312">
        <property type="protein sequence ID" value="AGZ44312"/>
        <property type="gene ID" value="AFR_30260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6127"/>
    <dbReference type="eggNOG" id="COG2114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085079_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="27151" checksum="EBB0DB4715511500" modified="2014-01-22" version="1">MVADMERYSARSSVLQNKAQTVFLDALDAAGLAADLDRGTWRRQASGDGELAVLPADVDEPALLSAFLLTLDKRLRAYNVERQPQARVRVRVSVHQGLLFPESRNGFAGEAVNTAARLVDAPVLKRALREFPDAAVACIVSDGIHRDVIAGGYEGIRPERFRRLRVVLGDKNFDEWAWLQIVDEDINNLDLQSATAESVPVVSERRPSREVPPGGIQVGDVDNRDGQVAIGHGATATGGVHQRIRFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5S5</accession>
    <name>U5W5S5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Riboflavin biosynthesis protein RibBA</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="13">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
                <shortName evidence="13">DHBP synthase</shortName>
                <ecNumber evidence="13">4.1.99.12</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="13">GTP cyclohydrolase-2</fullName>
                <ecNumber evidence="13">3.5.4.25</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="13">GTP cyclohydrolase II</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="13" type="primary">ribBA</name>
        <name evidence="15" type="ORF">AFR_30510</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 3 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 13">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the C-terminal section; belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 13">In the N-terminal section; belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="13"/>
    <dbReference type="EC" id="3.5.4.25" evidence="13"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560699.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30510"/>
    <dbReference type="EnsemblBacteria" id="AGZ44362">
        <property type="protein sequence ID" value="AGZ44362"/>
        <property type="gene ID" value="AFR_30510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6177"/>
    <dbReference type="eggNOG" id="COG0108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_1_2_11"/>
    <dbReference type="OrthoDB" id="900513at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00179">
        <property type="entry name" value="RibA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00180">
        <property type="entry name" value="RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01283">
        <property type="entry name" value="RibBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016299">
        <property type="entry name" value="Riboflavin_synth_RibBA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00505">
        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 13 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydro2" evidence="14">
        <location>
            <begin position="207"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="DHBP synthase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="13">
        <location>
            <begin position="26"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="13">
        <location>
            <begin position="139"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="GTP cyclohydrolase II" evidence="13">
        <location>
            <begin position="201"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for GTP cyclohydrolase activity" evidence="13">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GTP cyclohydrolase activity" evidence="13">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="251"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="295"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="site" description="Essential for DHBP synthase activity" evidence="13">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="site" description="Essential for DHBP synthase activity" evidence="13">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ44362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="43781" checksum="3F9E215B1B340EB9" modified="2014-01-22" version="1">MSVEIERAIKDIAAGRPVIVVDDEDRENEGDLIFAAEFATPELVAFMVRYTSGYICVPLPEEEADRLDLPPMFHTNQDRRGTAYAVTVDAREGISTGISAADRAHTIRLLAGADTQPTDLSRPGHVVPLRAKAGGVLRRPGHTEAAIDLSVLAGLRPAGVLCEMINDDGTMMRMPDLEKFAAEHDLALISIADLIAYRRHHESQVERVVETRLPTEHGDFTAVGYRTDVVGAEHVALVFGELGDGEDVLVRVHSECLTGDVFGSVRCDCGPQLDAALQRVAEVGRGVVLYMRGHEGRGIGLLHKLQAYQLQDRGLDTVDANLELGLPADARDYGTGAQILYDLGVRSMRLLTNNPAKRAGLEGYGLTITGREALPVRLHPENVRYLRTKRDRMGHLFEALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5V3</accession>
    <name>U5W5V3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Group 1 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560857.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31320"/>
    <dbReference type="EnsemblBacteria" id="AGZ44524">
        <property type="protein sequence ID" value="AGZ44524"/>
        <property type="gene ID" value="AFR_31320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6339"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_3_11"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="21"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="202"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="398" mass="42500" checksum="CD5975E740BAFE62" modified="2014-01-22" version="1">MRIAMISEHASPLPAGDAAGAQQRHVAELSTALAGLGHDVRVYTRRDDPKLPAIVKMGEGVCVVHVPAGPAMALPPAQLLSHMGDFAQWLRTEWRDSDWLPEVAHAHFWTSGLAAVTAARQVGIPVVQSFHELGVAGPAESGPSRAGYERALGRAVDRVVAQSQDEVRGLVRIGVPRARLTVVPAGVDSVRFTPDGPSAERDPERTRILSVGRLVERKGFGDVIQAMRYVPGAEYVVIGGPSADQLAADPQAKRLRALAEHYQVADRVRIVGSVPGKDMPRWYRSADLLVAAPWQDQFEPAALEAMACGVPVIGTAIGGISETVVDGLTGDLVPARDPRALGGALRRLVNDKVRRFAYATAALDRARQAYSWKRVASQLGSVYSAVAGRKPTEAEAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6A5</accession>
    <name>U5W6A5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560865.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44532">
        <property type="protein sequence ID" value="AGZ44532"/>
        <property type="gene ID" value="AFR_31360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6347"/>
    <dbReference type="eggNOG" id="ENOG5031T48">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_203204_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="69" mass="7551" checksum="FD7F0DA33676CECA" modified="2014-01-22" version="1">MRSAGFLSERQRRIAWVGFLLAAFQAPAAARLVDDASWLFAVVVALLVATVILADDAQRRRPEVAEPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6C2</accession>
    <name>U5W6C2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative sodium-calcium/proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_32130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561017.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32130"/>
    <dbReference type="EnsemblBacteria" id="AGZ44684">
        <property type="protein sequence ID" value="AGZ44684"/>
        <property type="gene ID" value="AFR_32130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6505"/>
    <dbReference type="eggNOG" id="COG0387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050648_1_0_11"/>
    <dbReference type="OrthoDB" id="988251at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="6">
        <location>
            <begin position="36"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="6">
        <location>
            <begin position="222"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="366" mass="37299" checksum="AC333006AAE06401" modified="2014-01-22" version="1">MTAKIAAVLARWTAWIPILAVAVLVVTFGRDLPPPLVIISGLLLAGAVLAAVHHAEVVAHKVGEPFGSLVLAVAVTIIEVALIVTLMISGGEKTQALARDTVFAAVMITCNGILGISLLVGAVRRRIAVFNPEGTGGALAAVATIATLSLVLPSFTTSRPGPQFSPAQLAFAAVASLGLYLLFVLVQTRRHRDYFMPITTAGEVIEGEDHLEPPSSKAALTSLGLLLVALIAVVGLAKGVSPAIESAVAAAGMPQAVVGVVIALLVLLPETIAAVRAAARDRMQTSLNLALGSAMASIGLTIPAIAIAMIWLDGPLLLGLGGTQMVLLALTVVVGTLTVVPGRANVLQGGIHLSLLAVFLFLAASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6F6</accession>
    <name>U5W6F6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1990 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561131.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32675"/>
    <dbReference type="EnsemblBacteria" id="AGZ44793">
        <property type="protein sequence ID" value="AGZ44793"/>
        <property type="gene ID" value="AFR_32675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6612"/>
    <dbReference type="eggNOG" id="COG4762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080841_1_0_11"/>
    <dbReference type="OrthoDB" id="1586328at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018960">
        <property type="entry name" value="DUF1990"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014457">
        <property type="entry name" value="UCP010260"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09348">
        <property type="entry name" value="DUF1990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010260">
        <property type="entry name" value="UCP010260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1990" evidence="1">
        <location>
            <begin position="5"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="18415" checksum="5CDB06D88EB4F27F" modified="2014-01-22" version="1">MTGFTYAEVGATRHEPLPPGYNRLHYRTELGRDDFRTAADAILTFAMHRATGARIRSSADRAAPGVRLTVGLGPLTVPCEVVWTEDGDELAGFGYGTLPGHQARGEESFTVSRDAQGRVWFRVTAFSLPARWPMRLGGPAAVFAQRLYAHRCGRALRQLCARSRTVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6P0</accession>
    <name>U5W6P0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_32070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561005.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32070"/>
    <dbReference type="EnsemblBacteria" id="AGZ44672">
        <property type="protein sequence ID" value="AGZ44672"/>
        <property type="gene ID" value="AFR_32070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6493"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_11"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="130"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="130"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="24419" checksum="51935619448C3E01" modified="2014-01-22" version="1">MSDKGLILLVEDERPIADLVRLYLTRDGFGVHVEHDGTAGLAAARRLRPVACVLDIALPGLEGTEICRRLREDGDWTPVVFLTARDDEVDRILGLELGGDDYLTKPFSPRELVARVRALLRRASGPPDGGRVRTLGPLQLDPGRRLATVDGSPLALTPTEFDLLGHLLARPGQVFTREQLLASVWGYAAHAGTRTVDVHVAQVRAKLGPAAGLIRTVRGVGYTADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6S4</accession>
    <name>U5W6S4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561039.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32245"/>
    <dbReference type="EnsemblBacteria" id="AGZ44707">
        <property type="protein sequence ID" value="AGZ44707"/>
        <property type="gene ID" value="AFR_32245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6527"/>
    <dbReference type="eggNOG" id="COG2021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_1_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="28261" checksum="1E21753A2073FDA2" modified="2014-01-22" version="1">MPAIGVGDITLHYEESGRGAPVLLIPGTAARGRTWWLHQVPALVAAGYRAITLDNRGVGKSGPAVPGLSIADLVGDTVAVIEQVVGGPCRLIGTSMGAYVVQELLVTRPDLVYRAVLMAGRARPDAVSATLAAAERALVAAGVVLPPEYDAVTRALQNLSPRTLEDPVTAQDWLDILEMSPTDWTDAGHRAQLGVTVDTDRRAAYRTITVPTLVMSFADDLIAPAVRGRELAAAIPGARYAEIADAGHYGYLEQPDAVNSALLAFLHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Z9</accession>
    <name>U5W6Z9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Beta-N-acetylhexosaminidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_27495</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27495"/>
    <dbReference type="EnsemblBacteria" id="AGZ43760">
        <property type="protein sequence ID" value="AGZ43760"/>
        <property type="gene ID" value="AFR_27495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5573"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008392_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665614" evidence="3">
        <location>
            <begin position="26"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_3" evidence="4">
        <location>
            <begin position="53"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="382" mass="39649" checksum="A851E963C290A4BE" modified="2014-01-22" version="1">MMIRSWWALPVVVLALVGPPAPVVSRPPATSTVSSPAGTFPACVTAAVETMSLEEQVGQTLMVGVPLHSPQGIGGLVAGYHLGGVFLSGRSTRSSAGLRADITALQRTAKVPLLVSVDQEGGSVQALQGTDFPDIPAATTLGAGPPDELRRTVRDSADRLAGIGVTVNLAPVADTVPSGTDDRNPPIGAFRRQYDSDPIKVAEDIRTVVAASQGAGVLTILKHFPGLGRVRANTDTSTRAVDRTATVDDPYLDPFRAGMRQDSAGVMISLATYPRIDPASIAAFSRPIVTGLLRERYAFTGLIMSDDLGAADAVESVPTGDRAVRFLDAGGDLALTIRPQNVGPMVAALLAAARTSPVFAARVTDAASHVLAAKHRAGLLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7D1</accession>
    <name>U5W7D1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Winged helix family two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_28220</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28220"/>
    <dbReference type="EnsemblBacteria" id="AGZ43905">
        <property type="protein sequence ID" value="AGZ43905"/>
        <property type="gene ID" value="AFR_28220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5720"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="24070" checksum="F17D0CD045D78BAF" modified="2014-01-22" version="1">MRVMVVEDDEDLRLAVTTELAGAGLTVDQTGAIGAADAALSTGDYDCVVFDRMLPDGDAITYVHRRRQEGWAVPVLFLTARDSLADIVAGFEHGGDDYLVKPFAVAELTARVLTLGRRAGTGRPSVLRHGDLEMDCARRQVRRAGVLLVLTSKEFAVLEYLLTRPEQAVPRTELIEHCWDSSTDPMSNVVDVVVGRLRAKLRKPDLIHTVRGRGYRLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7N0</accession>
    <name>U5W7N0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_28475</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560293.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28475"/>
    <dbReference type="EnsemblBacteria" id="AGZ43956">
        <property type="protein sequence ID" value="AGZ43956"/>
        <property type="gene ID" value="AFR_28475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5773"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043648_0_0_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014305">
        <property type="entry name" value="RNA_pol_sigma-G_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02960">
        <property type="entry name" value="SigX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="7">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="8">
        <location>
            <begin position="126"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="SnoaL-like" evidence="9">
        <location>
            <begin position="201"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ43956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="321" mass="36128" checksum="7E85565A8AEC4B1A" modified="2014-01-22" version="1">MDEFDRRLDPYRPELLAHCYRMLGSVHDAEDLVQETCLRAWRAKDQYDESRSSLRTWLYRIATNACLTALKGRSSRPLPSGLVTESDPRRPFVHGGEITWLQPLPDSSLRRVEPETATIDRGSLRLAFVAAVQHLSARQRAVLILREVLDFSALEAAEILSTTTASVNSSLQRARARLKEVGISEEQVAEPSEAEQRAWIDKYLKAFENADVEGLKQLLTDDVIMEMPPMVNWFTGRENYGVFMDWVFAANGTQWRFLPVGANGQPAFAAYNLGPSGTYELHTLQVLTVTRNGISRTSVFRDAEVFASFELPAVHPFPGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7R8</accession>
    <name>U5W7R8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">8-amino-7-oxononanoate synthase</fullName>
            <ecNumber evidence="4">2.3.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34960</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.47" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561584.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34960"/>
    <dbReference type="EnsemblBacteria" id="AGZ45248">
        <property type="protein sequence ID" value="AGZ45248"/>
        <property type="gene ID" value="AFR_34960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7074"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_2_11"/>
    <dbReference type="OrthoDB" id="479874at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008710">
        <property type="term" value="F:8-amino-7-oxononanoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="3">
        <location>
            <begin position="33"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="378" mass="38417" checksum="E20F10FC4F70B6FB" modified="2014-01-22" version="1">MAGWQEALERQARTRAKAGLTRTLRPRAADDSVVDLAGNDYLGLAAHPEVIAAAAEALHRYGLGATGSRLVRGSTDAHTALETDLAGWLGAEQALVYSSGYLANLGAVRALAGPGTLLISDRYNHASLIDGCRSSGAEVVVAPHADPAALATILDAHPGRETVFVTESVFSVDGDLAPLAELHAVTSAHDTLLLVDDAHALGILGDSGAGGVAAAGLAGKPDVIVTATLSKSLGGAGGVVAGPAALIRHLIDTGRTFIYDTAPPPAVVAGVRAALRITRAADDRRAVLVARADQTVSRLTAAGFPVTAPAAGVLSVTAPGPEAALTWAEDCRDRGIAVGCFRPPSTPDGSSRLRLTLNAGIAAEDFDRALDVIVECAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7T2</accession>
    <name>U5W7T2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28870</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560372.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28870"/>
    <dbReference type="EnsemblBacteria" id="AGZ44035">
        <property type="protein sequence ID" value="AGZ44035"/>
        <property type="gene ID" value="AFR_28870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5852"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="300" mass="32528" checksum="2322361D21304584" modified="2014-01-22" version="1">MGQPVIEVDRLNVSYGDFQAVKELSIQVEQGELYALLGTNGAGKTSALEVIEGHRKASSGTVRVFGESPADRRAVRPRMGIMLQESGFSADLTAEESVRLVGRLSGRSDSVDRVLGIVDLTRKAATRVGQLSGGEKRRLDFATAVYGRPELIFLDEPTTGLDIQSRDALWEAVEELRGNGSTVVLTTHYLEEAQQHADRIGLMHRGTLRTQGTVPELTRALPAAIEFSLSPGAPVPPLGAAQPAGLAYQIETFELQADLKRLLDWADQHDVDLHGLSAAPTRLDDVFRAIETDPVPTPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Z0</accession>
    <name>U5W7Z0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561600.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35040"/>
    <dbReference type="EnsemblBacteria" id="AGZ45264">
        <property type="protein sequence ID" value="AGZ45264"/>
        <property type="gene ID" value="AFR_35040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7090"/>
    <dbReference type="eggNOG" id="ENOG5032SI8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138456_0_0_11"/>
    <dbReference type="OrthoDB" id="1779307at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021398">
        <property type="entry name" value="DUF3037"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11236">
        <property type="entry name" value="DUF3037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13861" checksum="F86750D69A4E988C" modified="2014-01-22" version="1">MRIPYEYAIIRAVPRVERGELINVGVILYCQRLDFMAARTHLHEGRLLALDPNADLDGIRASLKSWEITCAGGADGGAAREMKLGERFRWLVAPRSTILQAGPVHMGLAVDPSAELDRLVELLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W874</accession>
    <name>U5W874_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561685.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35465"/>
    <dbReference type="EnsemblBacteria" id="AGZ45349">
        <property type="protein sequence ID" value="AGZ45349"/>
        <property type="gene ID" value="AFR_35465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7177"/>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027489_0_0_11"/>
    <dbReference type="OrthoDB" id="1746495at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="353" mass="37885" checksum="F2C6ACA9F7CCBCC6" modified="2014-01-22" version="1">MFRKSGELARACDVVNDVVARRDGSAFEPAMERLVRAIRKARAAELGDAVERLTPALDRVALSGGGALAQVVGAIAEENLSVLPVLVRRAAEAMEEAARFAALHREAIGEPPGSDFEPAIEPTLERVREAAPKMGLTGDEAVALAWTWFSGNDWVQPVLYLAQRKDVRVALPERERLTRVTTASTELLDTASWLLGLLQVLDDEPLIVLHRGTGRGYRVTISGVGDNFQLSTLLAARLMGPEAQGLLPGVPPTKAMIAAASDGEQEIRGGLVGQFNLVGADGQWIWNEGRPADIPVQDGVRVVVLDPLPYSRSWNSGRLYSRMSPTVTVDGPLEPAEAEAWLAKVAPANPTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8B0</accession>
    <name>U5W8B0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-binding factor A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">rbfA</name>
        <name evidence="3" type="ORF">AFR_35960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Binds 30S ribosomal subunits, but not 50S ribosomal subunits or 70S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RbfA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561784.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35960"/>
    <dbReference type="EnsemblBacteria" id="AGZ45448">
        <property type="protein sequence ID" value="AGZ45448"/>
        <property type="gene ID" value="AFR_35960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7279"/>
    <dbReference type="eggNOG" id="COG0858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089475_0_0_11"/>
    <dbReference type="OrthoDB" id="1971380at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00003">
        <property type="entry name" value="RbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000238">
        <property type="entry name" value="RbfA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023799">
        <property type="entry name" value="RbfA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020053">
        <property type="entry name" value="Ribosome-bd_factorA_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33515">
        <property type="entry name" value="PTHR33515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02033">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89919">
        <property type="entry name" value="SSF89919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00082">
        <property type="entry name" value="rbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01319">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="17218" checksum="175D25C5FF448A6E" modified="2014-01-22" version="1">MSDPAKTRRHAERIRELVASLVREIKDPRLGMLTVTDARITGDLRDAHVYYTVLGDQTEQAATAAALESAKGMLRTRVGHALALRHSPSLTFVLDNVQDNVKEIDDLLAAARHRDEEVQRLAAGKQYAGDPDPYKVEEDDEEDADERVGAREDQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8D8</accession>
    <name>U5W8D8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560528.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29650"/>
    <dbReference type="EnsemblBacteria" id="AGZ44191">
        <property type="protein sequence ID" value="AGZ44191"/>
        <property type="gene ID" value="AFR_29650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6010"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_3_11"/>
    <dbReference type="OrthoDB" id="1167152at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21394" checksum="4CFF4E94AEF60263" modified="2014-01-22" version="1">MILPLPPGDFQAYLFDCDGTIADSMPLHHVAWRQALEPWGAVFPEDLFYAWGGRTVADIIADLNAQQGLAMPVAKVAEQRERIFRALLPQISAVPGVLEHIVDAAGRVPCAVVSGSTRESVTASLDALGLREKFEFLVCAGDYVRPKPDPEAFLTAAALLGVAPADCVVFEDTDLGIQAATAAGMASVRVPPPSQRFVVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8E9</accession>
    <name>U5W8E9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Dihydrofolate reductase</fullName>
            <ecNumber evidence="3 6">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_35130</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15009"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="3 6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561618.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35130"/>
    <dbReference type="EnsemblBacteria" id="AGZ45282">
        <property type="protein sequence ID" value="AGZ45282"/>
        <property type="gene ID" value="AFR_35130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7108"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_5_0_11"/>
    <dbReference type="OrthoDB" id="1920912at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="PTHR48069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000194">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="8">
        <location>
            <begin position="2"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="160" mass="17344" checksum="49AADC92C5CAA699" modified="2014-01-22" version="1">MTIHLIWAEAAGGVIGAEGGIPWNVPGEQKIFRERTLGSTVVMGRTTWDSLPERFRPLPGRRNVVLTRQSSWSAAGAEVFGSINDLLKVHDDFWVMGGGAVYTAFLPLATHIVRTRIDLAVEGDTFAPRLGPEWQVTAGADLTSDTGVHYVVEDLTSGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8J5</accession>
    <name>U5W8J5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydro-rel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561864.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36360"/>
    <dbReference type="EnsemblBacteria" id="AGZ45528">
        <property type="protein sequence ID" value="AGZ45528"/>
        <property type="gene ID" value="AFR_36360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7358"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023620_0_0_11"/>
    <dbReference type="OrthoDB" id="1456605at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="41"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="358" mass="37918" checksum="E6A3240B62E98B75" modified="2014-01-22" version="1">MALHVRGTVLPDGEVRDLWLVGDRVTLEPVADAETISDGGFIVPGLVDAHCHLGIQYGAKPIDSLDQARQLAHTDRDAGVLALRDAGSPYPYPELDDEPGIPRLVRAGRHVAPTRRYLRDIGIEVDAADVAATVTAQAKAGTGWVKLVGDWIDRSVGDLAPSWDPATMAAAVEAAHAAGARAAVHTFSEEGVAQMVRAGVDSVEHGTGLSLDLIDEMARRGTALVPTMINIKTFGGIADQARPKFPGYADHMLALRDGFPQVVASAYEAGVPIYVGTDAGGSIRHGLAAEEMLLLHEAGIPAGDVLAAASWRAREWLGFPGLVEGGLADLVVYETDPRQDLRVVRAPQRIVLRGAVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8U3</accession>
    <name>U5W8U3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_30775</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560750.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30775"/>
    <dbReference type="EnsemblBacteria" id="AGZ44415">
        <property type="protein sequence ID" value="AGZ44415"/>
        <property type="gene ID" value="AFR_30775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6230"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_3_11"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="85"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="146"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="361" mass="38518" checksum="F5DC0386EDB0C62C" modified="2014-01-22" version="1">MTARRRLRTQLTLLYAVPFLVSGALLLTIPILQTRQTAPVGTVLPAEPVRVADAGDHVSRLVAVSGLGLAIMGLVSLILGWVIAGRFLHPLRTMTATARDISASNLHRRLGHTGRNDEFTELAATLDDLFARLEASFTSQRNFVANASHELRTPLTAERTLLQVALADPNATVDGLRTVCRDVLDLGVAQERLVDALLTLASSEQGLEHRTPFDLADLAAEVTASRAAEARRRGLTLTTELAPAPASGDPSLVVSLIANLVDNALRHNTSQGHLAVTTTTYDGFSRLTVSNTGAVIPPAEVDRLYEAFQRLSPQRLHAGAGHGLGLAIVRAIAAAHSATITTTPHLEGGLTIEIRFPAERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W951</accession>
    <name>U5W951_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_31030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560801.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31030"/>
    <dbReference type="EnsemblBacteria" id="AGZ44466">
        <property type="protein sequence ID" value="AGZ44466"/>
        <property type="gene ID" value="AFR_31030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6281"/>
    <dbReference type="eggNOG" id="COG0652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_8_1_11"/>
    <dbReference type="OrthoDB" id="1861282at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="3">
        <location>
            <begin position="113"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="28825" checksum="84628187C1575960" modified="2014-01-22" version="1">MSSIKDRQRAAARARLEKEMAERASSARKRRQRQAIIGSALAIVVIAGAAVWIVAALGGDDDKKPADAAAAPPAGTVACTWIPEDGSTGSKVKDVGTPPPNAPNVGKATMTMDTNLGAISADVDLAKAPCTGEAFTFLASKKFWDNTKCHRLTTEGIKVLQCGDPSATGKGWRQSDGSGGPSFRYAEENLPTNAKPAYPKGTIAMAKTSAPSSTGSQFFIVYEDTDLPADYTVLGTITKGLDIVEGVAKAGSDDANGKGDGHPKKEIDIKTLTMAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9D8</accession>
    <name>U5W9D8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_37525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562094.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37525"/>
    <dbReference type="EnsemblBacteria" id="AGZ45759">
        <property type="protein sequence ID" value="AGZ45759"/>
        <property type="gene ID" value="AFR_37525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7590"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_3_2_11"/>
    <dbReference type="OrthoDB" id="1498685at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="24189" checksum="25E42AEA0986B51C" modified="2014-01-22" version="1">MPTLDRQNDVFVLDLGDGENRFHPDWLTAVNAALDEVEQADGARALVTAATGKFYSNGLDLEWLGEHGDQFEAYVVSVHDLFARFLSLPMTTVAALQGHTFAAGAMLSLAHDFRVMRADRGYWCLPEVDIDIPFSRGMSALVQSRLAPQTAHEAMTTARRYGGPDALAAGIVDRITDEGAVRATAVEIAASRAPKAGDILGTIKTRMYATVLQTLRDKENPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9X2</accession>
    <name>U5W9X2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38425"/>
    <dbReference type="EnsemblBacteria" id="AGZ45939">
        <property type="protein sequence ID" value="AGZ45939"/>
        <property type="gene ID" value="AFR_38425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7773"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666450" evidence="1">
        <location>
            <begin position="28"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="61"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="27646" checksum="4265E2ED64851550" modified="2014-01-22" version="1">MRRPRAVLAVTLGFVLMTGAGIGVSYAQSVSVPAKPTAGVAGPKPRPPAQNYRIQRTTGTKSVGLTFDDGPDPVNTPKLLDLLKKYNVKATFCLVGHRVRDNKALVKRIAAEGHTLCNHSWQHLEDLRERDDAYAFRDLEATNEQLHKAAPGAKIKYFRAPYGNFSPRLTQFAAKLGMTALSWNVDDQCYLTAEYGKGAAMRKRMFDIVKRDTRPGSIILSHENLKPHTVAAYETILPWLKQNFTLVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA03</accession>
    <name>U5WA03_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase ydbC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38575"/>
    <dbReference type="EnsemblBacteria" id="AGZ45969">
        <property type="protein sequence ID" value="AGZ45969"/>
        <property type="gene ID" value="AFR_38575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7805"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="16"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="285" mass="30737" checksum="AA0694E941BAA063" modified="2014-01-22" version="1">MVTDDTFPLGDRSVRRIGFGAMQLAGPGAFGPPRDRDTALDVLRKAVELGVNHIDTAQYYGPDVVNDLIRTALSPYPEDLVLVSKVGAARDEAGRWLPALEPEQLRSGVQDNLRSLGVDRLDAVNLRLTDGGDDFDAQLDAMIALRDEGLIAGIGLSNVSREQLVHAVQRTEIVCVQNALNLVERESMALLRECQELGIGFVPFFPLGSAFGGVNRLLTHPDLIDVAARLEALPSQVALAWLLDLAPNVLLIPGTSSASHLADNLAARTVHLDDDARKVLDTITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA43</accession>
    <name>U5WA43_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fatty acid metabolism regulator protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32985"/>
    <dbReference type="EnsemblBacteria" id="AGZ44855">
        <property type="protein sequence ID" value="AGZ44855"/>
        <property type="gene ID" value="AFR_32985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6675"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1472225_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TetR_C_1" evidence="1">
        <location>
            <begin position="65"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="19934" checksum="4F33B73EC6AF066C" modified="2014-01-22" version="1">MVVLGQCDDPVTEALLGTGAPAQDRGQVSTEDLQLGLSMRRVASDLGVATMSVYRHVPGKDELLILTRPQPAPNGLRFVEWVLDALSGTRLSRHERLYVHIMLVSFVRGVASALEPEAAAVRETGLSAGEWLETQESPVETLTLPHLRELADADTFDLDLDFLVRFGLARLLDGLDLYVDGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA57</accession>
    <name>U5WA57_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative branched-chain amino acid ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_38875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38875"/>
    <dbReference type="EnsemblBacteria" id="AGZ46029">
        <property type="protein sequence ID" value="AGZ46029"/>
        <property type="gene ID" value="AFR_38875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7867"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="322" mass="33016" checksum="6091C181B6CE7C5A" modified="2014-01-22" version="1">MIAAVLIVALTYVLPPFRNYQLATVGAYLCVTAGLTVLTGLNGQLSLGHGALMATGAYTVAMVQEQLSGRGQWTLLVSVIAAILVTTVAGGIVGLAAARLRGPYLAGLTLAVAVVVPAITSTFDKTFNSDQGLSVYVDPPPLALGATFPYERWQAWLSWAGALLTMLVLATLVRGRFGRSLRAVRDDEAAARIAGVSVARTQVLAFVVSAACAGLGGALFAVLAQSVSPGAFPLSLSLFLVMAIVIGGLGSLAGAVWGAVLLVALPALTHSVTDSINVSPAVAQRLEGNLPLALFGITLVVVMLAAPGGIASLKPTYRRRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAG3</accession>
    <name>U5WAG3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561273.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33390"/>
    <dbReference type="EnsemblBacteria" id="AGZ44936">
        <property type="protein sequence ID" value="AGZ44936"/>
        <property type="gene ID" value="AFR_33390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6758"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="228" mass="24606" checksum="54842F03D1735946" modified="2014-01-22" version="1">MTEMLLEARDVVLSFGRTQALRGAELRVAAGEILAIMGPSGSGKSTLLHCLAGILVPESGEIHFDGRRIDNLGETGRSALRRDRFGFVFQFGQLVPELSAEENVALPLLLNGVRRTKALAEARPWFDRLGLDGLEERRSGELSGGQAQRVALARGLVARPRVLFADEPTGALDTLTGEQVMDLMVSSAREQGTTVVLVTHEPRVAAYADREVIVRDGRVHALTAEAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB51</accession>
    <name>U5WB51_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CdiA_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40945"/>
    <dbReference type="EnsemblBacteria" id="AGZ46443">
        <property type="protein sequence ID" value="AGZ46443"/>
        <property type="gene ID" value="AFR_40945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8289"/>
    <dbReference type="eggNOG" id="ENOG5032Y1P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1937242_0_0_11"/>
    <dbReference type="OrthoDB" id="1860235at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004549">
        <property type="term" value="F:tRNA-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13442">
        <property type="entry name" value="CDI_toxin_Bp1026b_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033806">
        <property type="entry name" value="CDI_toxin_Bp1026b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040559">
        <property type="entry name" value="CdiA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18451">
        <property type="entry name" value="CdiA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CdiA_C" evidence="2">
        <location>
            <begin position="63"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="16850" checksum="BB4292F054237C43" modified="2014-01-22" version="1">MPDGHPTRISANEDDGVRRSLEMENSAAATLAGNGYQVKQNPTQDEVAQARLATGDSGRPGSKPDYLLEGRVFDCYSPTKPTKNARGIWSEVQEKVEDNQTQRVVVNLEGWRGDMSALQEQFDAWPIERLKEVKAITPDGDVVQIIPPDRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBQ1</accession>
    <name>U5WBQ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative HXlR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40865"/>
    <dbReference type="EnsemblBacteria" id="AGZ46427">
        <property type="protein sequence ID" value="AGZ46427"/>
        <property type="gene ID" value="AFR_40865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8273"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_993619_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="111"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="31196" checksum="EC58414FA79B6CC6" modified="2014-01-22" version="1">MSVRRVELIDADCGIEQALGVLTDWWTFLVVRDIAGGVTRFEALQRSLGVSRRALTERLAALVSHGVLRREAYSEHPPRFDYLLTAKGEGLLPVLIALQDWGTRHVLGDGSLTATAAPTSAEAHRVHGLVGHLVPVVPISRWTVLYFFPGAYPSAADYPPGWGDIPGAGGCTLESLTYASRADDFAAAGVQVKGVSTQRPDQLEAFAAHAGLPFPLVSDQDAKVAAGLRLPTFRASGVDRFKRLSLLVDASATIRAVQYPITDPAASVTEMLELATGFGLARTQPAIGGSDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBV7</accession>
    <name>U5WBV7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative chitinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_41725</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562930.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41725"/>
    <dbReference type="EnsemblBacteria" id="AGZ46599">
        <property type="protein sequence ID" value="AGZ46599"/>
        <property type="gene ID" value="AFR_41725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8444"/>
    <dbReference type="eggNOG" id="COG3179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3979">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045506_0_0_11"/>
    <dbReference type="OrthoDB" id="1584918at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003610">
        <property type="entry name" value="CBM_fam5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036573">
        <property type="entry name" value="CBM_sf_5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00495">
        <property type="entry name" value="ChtBD3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51055">
        <property type="entry name" value="SSF51055"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666275" evidence="2">
        <location>
            <begin position="33"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-3" evidence="3">
        <location>
            <begin position="37"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-3" evidence="3">
        <location>
            <begin position="102"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="39255" checksum="769D9A6E284568C3" modified="2014-01-22" version="1">MSKKRLLSIVATVSVALAGAAAALLPMTASNAAEACVAAYNNSAVYTGTQRASYNGHNWTAKWWTQGETPSTGGSGVWADNGACGGGGGGTTPPPPGGNCNYPNWAAGQFYAAGSIVKYTNGNYYRAKYENPGYDPVISTWFWEVFNCSGGGNPTTPPPGNGSGNFPVSEAQFNQMFPNRIGFYSYSGLIDAIKKFPSFTTTGSDTVKKQEAAAFLANINHESGGLVYVEEINQANWPLYCDRSQSYGCPAGQSAYHGRGPIQLSWNFNYKAAGDALGIDLLNNPDRVKNESSVAYQTAVWYWMTQRGPGSMTPHDAMVNSRGFGETIRSINGSLECNGGNPAQVQSRVDAYNRFTSILGVSPGGNLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBY2</accession>
    <name>U5WBY2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561841.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45505">
        <property type="protein sequence ID" value="AGZ45505"/>
        <property type="gene ID" value="AFR_36245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7334"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140176_17_1_11"/>
    <dbReference type="OrthoDB" id="2076478at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="61" mass="6898" checksum="5EEF8991F5DA6804" modified="2014-01-22" version="1">MGAHEIRERLGGVSRQRAYQITSRADFPAPVADLAQGKVWSTDDVEAWMKVHRKEQDDTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCQ5</accession>
    <name>U5WCQ5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VanZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_37655</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562120.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37655"/>
    <dbReference type="EnsemblBacteria" id="AGZ45785">
        <property type="protein sequence ID" value="AGZ45785"/>
        <property type="gene ID" value="AFR_37655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37655"/>
    <dbReference type="eggNOG" id="COG4767">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1700468_0_0_11"/>
    <dbReference type="OrthoDB" id="1262421at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006976">
        <property type="entry name" value="VanZ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04892">
        <property type="entry name" value="VanZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666517" description="VanZ domain-containing protein" evidence="2">
        <location>
            <begin position="36"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="VanZ" evidence="3">
        <location>
            <begin position="12"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="154" mass="16792" checksum="12BB7B4B1EA82DB6" modified="2014-01-22" version="1">MMRRRVTTTILLLYAVSVAAVTVLPIRAHPAAYWAAEPWWTVFHWIPGVVDGPSFVLNVIMFIPLGVLLPLLRPRLDAVRRLTAYAAGASFSIELTQFVLGMTLGSRRTVDVNDLIANTAGALVGLLILRLALPSRRRRATCVAGKPSSPPPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD68</accession>
    <name>U5WD68_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">4-alpha-glucanotransferase</fullName>
            <ecNumber evidence="3 7">2.4.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Amylomaltase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6 7">Disproportionating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_38210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="2.4.1.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the disproportionating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.25" evidence="3 7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562231.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38210"/>
    <dbReference type="EnsemblBacteria" id="AGZ45896">
        <property type="protein sequence ID" value="AGZ45896"/>
        <property type="gene ID" value="AFR_38210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7730"/>
    <dbReference type="eggNOG" id="COG1640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022072_1_0_11"/>
    <dbReference type="OrthoDB" id="814704at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102500">
        <property type="term" value="F:beta-maltose 4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003385">
        <property type="entry name" value="Glyco_hydro_77"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32438">
        <property type="entry name" value="PTHR32438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02446">
        <property type="entry name" value="Glyco_hydro_77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00217">
        <property type="entry name" value="malQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="648" mass="70961" checksum="793E2762D6870847" modified="2014-01-22" version="1">MDADLTALAEAHGVATWYEDWHRKRRDVAAESVIGVLGLLGVDATSPESIAEELAAIRTAREQHRLPGTVIVRQGATRPLTGTVHLEDGSRVEATGQLPDDLPLGWHRLVTDDQDVALVVVPTELPEPPETWGWMLQLYALHSAASWGLGDLGDLRTFVEGSRGAGLVLLNPLHAVTPTLPVPASPYSPSSRRFANPLYLRVADTDAYRRADPPLREQVDALRPAPTADGLIDYTAVWQAKRAALQLLWPLAGEVDLTADPGLTDFATFCALAETHGPNWQTWPEEFRRPDSPAVQAARGGDRVAFHVWLQRLLDDQLNAANQAARDAGMPVGIVHDLAVGIDPNGADGWLLQDVLAAGVHTGAPPDAFNQLGQDWGLAAWRPDQLIETGYAAYRDMLQRIFRRAGGLRVDHVAGLWRLWWVPPGLGPAEGTYVHYDPEAMLGILALEAHRAGAVVIGEDLGTVQPVVTETLERMNMLRSAVLWFTRDYENDPTQQYLPAADYPRNALASISTHDLPTAAGFLVGEQVRVRAELGQLAGTIEEEQDYADKDRAKLLFRLTEEGLITPDSGPDEIIVAMHQFLARTPCRFITASLYDVLGELRQPNLPGTTDEYPNWRIPLTSSLEQILADPRVDRVAEVLSAQKGVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEC6</accession>
    <name>U5WEC6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562722.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40680"/>
    <dbReference type="EnsemblBacteria" id="AGZ46390">
        <property type="protein sequence ID" value="AGZ46390"/>
        <property type="gene ID" value="AFR_40680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8233"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_2_0_11"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="28915" checksum="D6C4320A11211EBC" modified="2014-01-22" version="1">MTGTLITVAPTGAESSKAAVPALPVTIDELVATAKECEALGAAIVHVHIRDEAAEPTLDRGRLRDTVAALRQSTDLIVQLSSGGAVTDPEGDRLAVLDAGPEMASCTMGTVNFGDGVFLNRWEFIVDLHSRMQERGIVPEYEIFDLGQLTTLQRLLGQYGLPAGGHVHVDLVMGVPGGMPGTAGALVACEQVIRDLPAGTTFSATGIGRSTIPVMLASLSAGGHLRVGMEDTITYAKGRAVESNMQLVARAVGFAQLAQRPPLTTAEARDLLGVPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEC8</accession>
    <name>U5WEC8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Redox-sensing transcriptional repressor Rex</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rex</name>
        <name evidence="8" type="ORF">AFR_40185</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Modulates transcription in response to changes in cellular NADH/NAD(+) redox state.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the transcriptional regulatory Rex family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562623.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40185"/>
    <dbReference type="EnsemblBacteria" id="AGZ46291">
        <property type="protein sequence ID" value="AGZ46291"/>
        <property type="gene ID" value="AFR_40185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8133"/>
    <dbReference type="eggNOG" id="COG2344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061534_0_1_11"/>
    <dbReference type="OrthoDB" id="1872374at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051775">
        <property type="term" value="P:response to redox state"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01131">
        <property type="entry name" value="Rex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009718">
        <property type="entry name" value="Rex_DNA-bd_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022876">
        <property type="entry name" value="Tscrpt_rep_Rex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35786">
        <property type="entry name" value="PTHR35786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06971">
        <property type="entry name" value="Put_DNA-bind_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CoA_binding" evidence="7">
        <location>
            <begin position="95"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="32"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="106"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="25339" checksum="DBD68C9AA44D262A" modified="2014-01-22" version="1">MSQQRHGNTPRNAGAVPAFPDLPEATIARLPEYLRALHNLAEAGSETVSSEGLAAAAGVNSAKLRKDLSHLGSYGTRGVGYDVALLIDQIEYILGLDQRRAVALVGVGNLGHALAGYDGFASRGFRIVALFDADADQVGKPLNGLTVRHIDELGQAVVEEGITIGVIATPAGAAQAVADDLVAAGVTSILNFAPCVLSVPAGVDVRKVDLAIELQILSFHEHRKASLTAIPGGRSAPAGNQEAVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEZ0</accession>
    <name>U5WEZ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-alkyl-2-methoxyhydroquinones prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_41310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562848.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41310"/>
    <dbReference type="EnsemblBacteria" id="AGZ46516">
        <property type="protein sequence ID" value="AGZ46516"/>
        <property type="gene ID" value="AFR_41310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8360"/>
    <dbReference type="eggNOG" id="COG0382">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080174_0_0_11"/>
    <dbReference type="OrthoDB" id="1740841at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="28189" checksum="B0118F1FDC94CDA9" modified="2014-01-22" version="1">MSGPVSLLKASHPEPGGAVTLAMTLLAIGVGHRGWAVLGVAAAIGATQLSVGWVNDWLDADRDRVAGRTDKPVASGVVSRRTVGISGLIASLAIPLLGLPFGAAATVCITLVGIFALLYDWPLKSTALSVVPYLVAFGLMPAFVVIALPGHPWPPAWLVAAGALLGGGAHFANVLPDLADDAATGVVGLPHRIGATWSRIAAGGLLLAATLTLVFGPPGPPSWPGIAAAAAAVVVLPLGWYAGRREAARGARQVAMFRSVIVVALLDVVLLVFSGRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFM1</accession>
    <name>U5WFM1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transketolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42335"/>
    <dbReference type="EnsemblBacteria" id="AGZ46721">
        <property type="protein sequence ID" value="AGZ46721"/>
        <property type="gene ID" value="AFR_42335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8568"/>
    <dbReference type="eggNOG" id="COG3959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="1">
        <location>
            <begin position="29"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24682" checksum="9419A886CFCCFB40" modified="2014-01-22" version="1">MQSENMTVTAVRPDLLGTLLTRVTGDEKHAPSAFSTLDVLWVLYDRILRVTPETVDEPDRDRFLLSKGHGPAAYYAVLAANGFIPVDWLDDLGGADSHLGHHPDRVLIPGVEIGTGSLGHGLGLAVGTALGLRAQGFDSRTFVLVGDAELDEGSNHEAIAYAAAARIRSLTAIVIDNQSSTHGWPGGIASRFPGWTTSIVDGRDHDAIEHALSEKDRDRPHVVVAAVEKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFP4</accession>
    <name>U5WFP4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium/glucose cotransporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_42460</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563077.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42460"/>
    <dbReference type="EnsemblBacteria" id="AGZ46746">
        <property type="protein sequence ID" value="AGZ46746"/>
        <property type="gene ID" value="AFR_42460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8593"/>
    <dbReference type="eggNOG" id="COG4147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_8_1_11"/>
    <dbReference type="OrthoDB" id="278483at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="545" mass="57212" checksum="238CB8EC31302557" modified="2014-01-22" version="1">MNPYVVPGLILVVAVTVAIGAYGLRFARTTSDFLVASRSVSPTWNAAAISGEYLSAASFLGVAGLILRYGVDVLWYPVGFAAGYLALLLFVAAPLRRSGAFTLPDFCQVRLRSPLLRRLATIFVVFIGCLYLVPQLQGAGLTFTTVTGGRYEWGALLVGLVVTANVALGGMRAITFVQAFQYWLKLTALAVPVIFLILQWQADGRPAVTPPAGPAFSATTTVRFEEAATLTLPDGSESRVAAGEERTFAAGTPVPDVDKVDDRRGVEWLMPGGDATLFATYSLILATFLGTMGLPHVLVRFYTNPDGASARRTTLVVLALVGLFYLLPTLYGVLGRIYTPQLLMTGDTDAVVLLLPEAALGSGHLGQFLGALVTAGALAAFLSTSSGLLTSVAGVVFTDILHADRGGSVRDFRVATVLAAIVPMALSFYVANMDVSRVVGLAFAVAASSFCPLLVLGIWWRGLTDVGAIAGVLAGGGAAMGAVLLTVLGPPLTGWPAQLIGQPAAWTVPLAFLVMVVGSLLTKRRVPTDIGATMLRLHAPESLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNM6</accession>
    <name>U5VNM6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">K(+)/H(+) antiporter subunit khtT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357343.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00550"/>
    <dbReference type="EnsemblBacteria" id="AGZ38399">
        <property type="protein sequence ID" value="AGZ38399"/>
        <property type="gene ID" value="AFR_00550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.112"/>
    <dbReference type="eggNOG" id="COG0490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116143_1_0_11"/>
    <dbReference type="OrthoDB" id="941006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026278">
        <property type="entry name" value="K(+)/H(+)_antiporter_KhtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005028">
        <property type="entry name" value="KhtT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK C-terminal" evidence="1">
        <location>
            <begin position="77"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="17268" checksum="8355230DF597EFE8" modified="2014-01-22" version="1">MRVRVEQTPLPGIGVRHDLITSSGRTVGVVSHRNGRRDLLLYDVDDPDSCLASIPLTDDEAEALADVLGASLMLGQLAGLRQQAAGLLTEQIALPAGSPFVGRRLGDTRARTRTSASIVAVLRDREVIASPGPSFQFDANDVVVAVGTREGLNGVTAILAGDTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNT7</accession>
    <name>U5VNT7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357541.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01545"/>
    <dbReference type="EnsemblBacteria" id="AGZ38598">
        <property type="protein sequence ID" value="AGZ38598"/>
        <property type="gene ID" value="AFR_01545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.310"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142189_1_0_11"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027395">
        <property type="entry name" value="WH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13601">
        <property type="entry name" value="HTH_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_34" evidence="1">
        <location>
            <begin position="27"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12915" checksum="39880AF1949A3A5B" modified="2014-01-22" version="1">MTDGTRPPIEDGGGIPELDPVIHAQARLRVVSALSTLREADRIAFPRLQDILQMTAGNLSVHLRKLEDAGYVEVTKTHQGRTPATLVRLTRRGRLAFEDYTAALRALLAGGTNLQEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNX6</accession>
    <name>U5VNX6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357445.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01065"/>
    <dbReference type="EnsemblBacteria" id="AGZ38502">
        <property type="protein sequence ID" value="AGZ38502"/>
        <property type="gene ID" value="AFR_01065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.212"/>
    <dbReference type="eggNOG" id="COG3012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039623_0_0_11"/>
    <dbReference type="OrthoDB" id="1177251at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="333" mass="35506" checksum="4D4623684EA784F2" modified="2014-01-22" version="1">MPTKADLSSADLDEIRQSALGAADPLGVAAELAGAVDAGRLADPGDAGHALTLAAEIAESRSKLDAALRYADRAIDAYGTRDDSQVAAVHALRARILFRAGRGDDAMAELEPLRPLLTRYSDAAAYVSAALVVGGRTRVAEEWLTEAVKVALDERGGSAEPASADDAGLLFFLLQQRHRTRHALGLPHDQHDNLADRLETKLANTTARAATASAEDLVFWPQAEFDRLLAEQPKLSEAYGETWDEHRAHLERELVRLAGAGRTGLALLPGSVTGLIRYAGSDGDPSDPQTRSGYAKQLAAAPGHISWPPERNGSCWCGSGNKYKKCCLPRSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPA1</accession>
    <name>U5VPA1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ESAT-6-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WXG100 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02520"/>
    <dbReference type="EnsemblBacteria" id="AGZ38793">
        <property type="protein sequence ID" value="AGZ38793"/>
        <property type="gene ID" value="AFR_02520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.506"/>
    <dbReference type="eggNOG" id="COG4842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151185_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036689">
        <property type="entry name" value="ESAT-6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010310">
        <property type="entry name" value="T7SS_ESAT-6-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06013">
        <property type="entry name" value="WXG100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140453">
        <property type="entry name" value="SSF140453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03930">
        <property type="entry name" value="WXG100_ESAT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="102" mass="10855" checksum="3F36732D59A00C1A" modified="2014-01-22" version="1">MVQTTEPGMQAAAQEFSARATEFTGHLQSVNSEMATLQSTWTGTASNGFNQAMDNWEASFQKVINELINIMEVMGVNTKGYAQAEDTAASSAQSFASALPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPJ9</accession>
    <name>U5VPJ9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Citrate synthase (unknown stereospecificity)</fullName>
            <ecNumber evidence="3">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_02200</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357672.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02200"/>
    <dbReference type="EnsemblBacteria" id="AGZ38729">
        <property type="protein sequence ID" value="AGZ38729"/>
        <property type="gene ID" value="AFR_02200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.445"/>
    <dbReference type="eggNOG" id="COG0372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025068_2_1_11"/>
    <dbReference type="OrthoDB" id="1412360at2"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="PTHR11739"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="368" mass="39896" checksum="69FAE7E29CE197C0" modified="2014-01-22" version="1">MSDFKPGLEGVIAFETEIAEPDKAGGSLRYRGVDIEDLIGQVSFGNVWALLVDGRFGPGLPPAEPFPVPVHSGDIRVDVQSAVAMLAPYWGLDQMLDIDEDQTRADLARVSVTALSFVAQAARGLGLPAVPQKDIDKAQTIVERFMKRWRGEPDPRHVKAVDAYFISAAEHGLNASTFTTRIVASTGADAAACISAGIGALSGPLHGGAPSRVLHMIEAVERSGDAEGYVKGVLDRGERLMGFGHRVYRAEDPRARVLRRTAKELGAPRFEVAEALEKAALAELQARKPDRVLMTNVEFWSAVVLDFAEVPAHMFTSMFTCARVAGWSAHILEQKKLGRIVRPSALYVGPDTRKPQDVEGWDAVPHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ35</accession>
    <name>U5VQ35_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="7">SHMT</shortName>
            <shortName evidence="7">Serine methylase</shortName>
            <ecNumber evidence="7">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glyA</name>
        <name evidence="10" type="ORF">AFR_03190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357870.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03190"/>
    <dbReference type="EnsemblBacteria" id="AGZ38927">
        <property type="protein sequence ID" value="AGZ38927"/>
        <property type="gene ID" value="AFR_03190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.642"/>
    <dbReference type="eggNOG" id="COG0112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_11"/>
    <dbReference type="OrthoDB" id="861782at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SHMT" evidence="9">
        <location>
            <begin position="20"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="SHMT" evidence="9">
        <location>
            <begin position="154"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7 8">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ38927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="473" mass="50613" checksum="ED9AD90F0E916999" modified="2014-01-22" version="1">MSALNAESTAFRSALDAVRAVEPRIADAIAKELTDQRESLKLIASENYASPAVLLAMGNWLSDKYAEGTIGRRFYAGCQNVDTVESVAVEHAKALFNAPYAYVQPHSGIDANLVAYWAILADRVEAPALAKVQKRNVNDMTDAEWAELRAAFGNQRMLGMSLDAGGHLTHGFRPNISGKMFDQRSYGVDPATGQIDYAALRTSAQEFKPLVIVGGYSAYPRKVNFRIMREIADEVGATFMVDMAHFAGLVAGKVFTGDFDPIANAHIVTTTTHKSLRGPRGGAVFCQPELSDQVDRGCPMVLGGPLAHVMAAKAVAFAEARRPEFADYAQRIVDNSQALAAGLTSRGATVVSGGTDNHLVLIDVDKYGLTGRQAEQALLDSGIVTNRNSIPQDPNGAWYTSGIRIGTPALTTRGLGTGEMDQIAELMHTVLTSTKAGASKAKFDLATAVSDRVSKQATELLADFPLYPSVDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ76</accession>
    <name>U5VQ76_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357907.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03375"/>
    <dbReference type="EnsemblBacteria" id="AGZ38964">
        <property type="protein sequence ID" value="AGZ38964"/>
        <property type="gene ID" value="AFR_03375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.679"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_11"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="26905" checksum="5ABB238BC844404E" modified="2014-01-22" version="1">MKLLQGRVVLVSGGTQGIGAGVARAAAREGASVVVTGRRPESGEPLARELGGTYLQSDVSDVDSARTSARATIERHGRIDCLVNAAGLTSRGTLLDTTPELFDQHIAVNLRGPFFLMQAAVQDMVRRGEPGTIVNIISSSELGGQPYLAPYVAAKAGLAGLTRNAAHAHRFDRIRINGLDIGWTDTEGEDVTQRAFHAADDGWRERAAKNLPMGKLSQVDEIADFVVFLLSTRSGVVTGSVIDWDQNVLGGMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQV4</accession>
    <name>U5VQV4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ribosomal RNA small subunit methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358229.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04675"/>
    <dbReference type="EnsemblBacteria" id="AGZ39224">
        <property type="protein sequence ID" value="AGZ39224"/>
        <property type="gene ID" value="AFR_04675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.944"/>
    <dbReference type="eggNOG" id="COG2813">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_7_0_11"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="31"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="21936" checksum="5E321ABA5FC6C80B" modified="2014-01-22" version="1">MTADHYFSADPAAPGRRSRIEFTAAGKAYDLAVAGGVFSAGRLDPGTAVLLRKGDLPSATTGGTFLDLGCGYGPIACVLASEAPDATVWAIDVNTRARELTEENAKTLGLAGRVRVAAPDDVPGEVLFDQIWSNPPTHVGKAELHTLLERWMPRLRPGGTAWIVINRNLGGDSLHTWLTSWGWDVARTASQRGFRVLRVTRKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRL3</accession>
    <name>U5VRL3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00405"/>
    <dbReference type="EnsemblBacteria" id="AGZ38370">
        <property type="protein sequence ID" value="AGZ38370"/>
        <property type="gene ID" value="AFR_00405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.83"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2462142_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="88" mass="10044" checksum="A6A354E01888F7A8" modified="2014-01-22" version="1">MLIDIEAAMFYDVEWEHAFLELRFGPHYPALRTVPLDPARLSFYRLVQYLSLVAGPLLLIDGDFPNAQVMRDIAEDNVRRALGEVHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRN1</accession>
    <name>U5VRN1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359134.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06570"/>
    <dbReference type="EnsemblBacteria" id="AGZ39603">
        <property type="protein sequence ID" value="AGZ39603"/>
        <property type="gene ID" value="AFR_06570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1332"/>
    <dbReference type="HOGENOM" id="CLU_2766529_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="69" mass="6893" checksum="DA4BF2C3581A392C" modified="2014-01-22" version="1">MGKKPTVNIASGNDTVPVQAGKVTGRPAKKDPAKPAKTAKGGGATNVVSGNARVGMQSDRVNGSISITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRQ7</accession>
    <name>U5VRQ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359164.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06720"/>
    <dbReference type="EnsemblBacteria" id="AGZ39633">
        <property type="protein sequence ID" value="AGZ39633"/>
        <property type="gene ID" value="AFR_06720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1373"/>
    <dbReference type="HOGENOM" id="CLU_1987850_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13291" checksum="484583AB41EAF2B1" modified="2014-01-22" version="1">MQISRSTALKIAAVLSVLVALIGIGLFDLPNLVLGAEKSEAPFALVLGSFTSDVLALIAAYGAWRGQKWGAVLLIVVNAFWVLQAVTSLLFDSTAAELTFAAVMLVHHAVVITLCLWRERVVSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRZ0</accession>
    <name>U5VRZ0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="4 9">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_06315</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O + nicotinate = ADP + diphosphate + nicotinate beta-D-ribonucleotide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="4 9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06315"/>
    <dbReference type="EnsemblBacteria" id="AGZ39552">
        <property type="protein sequence ID" value="AGZ39552"/>
        <property type="gene ID" value="AFR_06315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1281"/>
    <dbReference type="eggNOG" id="COG1488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025154_3_0_11"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006405">
        <property type="entry name" value="Nic_PRibTrfase_pncB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="PTHR11098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01513">
        <property type="entry name" value="NAPRTase_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NAPRTase_N" evidence="11">
        <location>
            <begin position="9"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="10">
        <location>
            <begin position="138"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ39552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="429" mass="45214" checksum="49895D40E3830CCC" modified="2014-01-22" version="1">MTTPFAPALLTDQYELTMVSAALKDGTADRRSVFEVFARRLPSGRRYGVVAGQGRLMELIRDFRFTDEDITFLRGAGVVDETTAAWLADYRFTGDIDGYAEGELFFPGSPILTVSGGFAECVVLETLILSVLNHDCAIAAGAARMVTAARGRPIIEMGSRRTHEEAAVAAARAAYLAGFASTSNLAAGGRYGIPTTGTSAHAFTLLHDDEPAAFASQVAALGKNTTLLVDTYDIAQGIRNAIAVAGPDLRAVRIDSGDLSVLAQHSRELLDSLGATETKIIVSGDMDEYSIATLAAEPVDMYGAGTAVVTGSGAPTAGLVYKLVEVEGRAVVKRSENKATVGGRKTAIRRHKPTGTATEEIVVSQGVPDHVANDRLLQRSFVVGGEVTALPTLAESRDHLRQCLISIPWEGLKLSAGDPAIPVTVVPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRZ1</accession>
    <name>U5VRZ1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PRK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359279.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07295"/>
    <dbReference type="EnsemblBacteria" id="AGZ39748">
        <property type="protein sequence ID" value="AGZ39748"/>
        <property type="gene ID" value="AFR_07295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1489"/>
    <dbReference type="eggNOG" id="COG0572">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087906_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRK" evidence="2">
        <location>
            <begin position="69"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="202" mass="22673" checksum="A356E6EE1D59D6CA" modified="2014-01-22" version="1">MTTETYAALAQRILATEPRLGGVRLVAVDGPSGAGKSWFAGRLAKVANIPVVHTDDLLDGWEDQFSFWGRLEEKVLEPLRRGRAATYQRYLWHRQAFGGTPVTVEPAACVLLEGVTAARREIRDELSFSVFVTAPADLRWRRALERDGRHDVAFRAYLERWRVNENEHFAADATAAHADLLVDGAAEGDDDWYERLWPAPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS88</accession>
    <name>U5VS88_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Xenobiotic-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_01355</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357503.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01355"/>
    <dbReference type="EnsemblBacteria" id="AGZ38560">
        <property type="protein sequence ID" value="AGZ38560"/>
        <property type="gene ID" value="AFR_01355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.271"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="314"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="348"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ38560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="570" mass="61046" checksum="8B27B11D34239759" modified="2014-01-22" version="1">MFVLIIGQAGVIAALGLSQLWLVDSSAAHRTGAVAGAVALGTLAYGVSAVAGRVQSNLWIYLVGRVRGRLNEETQRAISSIPTITHVEHGPYIDRWNRLFKNSQSLAAMPWSTLDAAAAVVSLAVTIGLLVWVGPVLCLLAVLAVPLFFANRHSDRLLRRSRDDNTELLRREQRLHELCIEPEPAKEVMLAGTGEILNDRASALWREAAAAETRARLRAAAAQAGAWLLYAAGVAAALLVVADLIGDGRATVGAAVLVVSLATQLQAQLRTVLDSLSTVAEAGQVVDHFWWLRRYAEEAARPGTPAPDVLGGGITLHDVRFRYPSADTDVLHGISLHLTPGSTVAIVGANGAGKSTLIKLLTGVYEPTGGHITVDGEPLAGIDRSGWRDRLSGVYQDFARIRLLTRETVGLGSVRHIRDRPSVTAAVDRAGAADVLERLPQGLDTQLGAAFGGVEPSLGQWQRLALARSLMREVSGDRPPLCVVLDEPTAALDPLAEHDLFQHFVEQVRRATRQGAVTVLVSHRFTTVRMADVIVVLEDGQVAEHGTHAELMAAGGGYAELYALQERAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSA5</accession>
    <name>U5VSA5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polycystic kidney disease protein 1-like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01455"/>
    <dbReference type="EnsemblBacteria" id="AGZ38580">
        <property type="protein sequence ID" value="AGZ38580"/>
        <property type="gene ID" value="AFR_01455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.293"/>
    <dbReference type="eggNOG" id="ENOG5030HT8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_734940_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="376" mass="37139" checksum="836B75D486A8DE0A" modified="2014-01-22" version="1">MPPSANAARATVPGSIDPAGARATTWPSGQAKVYGARPQEDAEQPAGDKADKPAAVSWMNVPEKPKAEDKPQGAEPEPDLPEPSTPEPSQPAPAKPEPTRPEPAPLPTPSPTPPSPPVPTPGPTPSPAPSPSPTPPGPVPPGPGPSPIPPGPGPSPIPPGPGPNPIPPGPGPNPVPPGPGPSPVPGPPPAPFPGPPMIPSAGGGLPAYTMPPSSGPAAVSGPFAPGGAQQDSQPRSGSTSYGVGASGGARQPFGGPAAQVSTPPARSTTYGSGSGQQSSAVPQQRGQGTVYGGLADYAPADMTMPVSTNPVENSGSLTGHILAQGWRDGPDTQRRSNVKVVVAMLVVLLVLVGVSLAFLFTVGDAFSDMIGGVFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSL0</accession>
    <name>U5VSL0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08445</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359509.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08445"/>
    <dbReference type="EnsemblBacteria" id="AGZ39978">
        <property type="protein sequence ID" value="AGZ39978"/>
        <property type="gene ID" value="AFR_08445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1719"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008392_5_3_11"/>
    <dbReference type="OrthoDB" id="949956at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="SSF52279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666024" evidence="4">
        <location>
            <begin position="25"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_3" evidence="5">
        <location>
            <begin position="36"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_3_C" evidence="6">
        <location>
            <begin position="409"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="565" mass="58180" checksum="5651FB2D299CA4F9" modified="2014-01-22" version="1">MLRRIVTIGSVLLLAVLPAGPARARDPIAEIMAGLTLPEKVGQMVVSYVYGADATTPSAANETMFGAGVRTGAEAVAKFHLGGVIYFTWSGNLVDPAQIAGLSNGLQRAAAADSGIPLQVSTDQEGGVVNRIGTPLAISPGNMAIGATFRPGDAFRAARVSGTELRALGINVVDAPVVDVNTNPRNSADGPRAFGDRTRQVSVFGASAVAGYRAAGIGTQAKHFPGLGDTTVNTDNGVAVTDETREQILATHVPPFRAALAAGAQSVMAAHIVAPALDPSGRPASLSKPVVTGLLRDRLHFDGVVITDALDAAALADIPDEQIVLDAVDAGVDQLLMPRDVQGAVRTLLDAVADGTISEARIDRSVRRILRMKSGLGLFRDPYVPAPVVGTPEHLSVMADVAGRSITQLRNTELPLSAGRKVLVTGWGASTTTNLTAGLVARGMDTTRFYTGSPSAAVIAQAVAAAREADVTVVTTYNAWGDGTQQALVAALLTTGKPVVVAAAGGPYDIAYLPGVTTYLAAYGYQPPSVTALADVLTGRTAARGRLPVTIRSADGSEVLFRYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT04</accession>
    <name>U5VT04_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357724.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02460"/>
    <dbReference type="EnsemblBacteria" id="AGZ38781">
        <property type="protein sequence ID" value="AGZ38781"/>
        <property type="gene ID" value="AFR_02460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.497"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030155_1_0_11"/>
    <dbReference type="OrthoDB" id="1033030at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021067">
        <property type="entry name" value="Glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34496">
        <property type="entry name" value="PTHR34496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11397">
        <property type="entry name" value="GlcNAc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="34562" checksum="BBEA31B11CA3BCFC" modified="2014-01-22" version="1">MTIFVSVAAYRDPELVPTVLDCLAKARHPEQLRIVVCWQHFGDEDVSPLTSHPRVELLDFDARESRGACWARAEIMRRYAGEDWFLQVDSHTRFAPGWDVRLIELAAATGADKPLLSCYPPTYEPDAAFTGAGQPTQITFDRWTGDGLPVFGQEHIPDWELRETPVPAHYIAAGFLFAPGSFVHEVPYDPGIYFHGEEITISARAFTWGYDLFHPAEVLSWHYYIRQDNPRHWTDHLDSPAGKGWFAMDRASRRRVANLLRFPSVGRYGIGPKRTMTEYETHIGVDFRNRKRTAADPRYVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT30</accession>
    <name>U5VT30_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Integrase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_09120</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09120"/>
    <dbReference type="EnsemblBacteria" id="AGZ40113">
        <property type="protein sequence ID" value="AGZ40113"/>
        <property type="gene ID" value="AFR_09120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1855"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_17_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="5">
        <location>
            <begin position="42"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="4">
        <location>
            <begin position="142"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="366" mass="40445" checksum="82464D7FE3A7B16E" modified="2014-01-22" version="1">MDNVGSSRERLFERKADADRYDANVRADLSRGEYIDERSGLISVAELAERWRADQLHIESTAIHVEHAIRLHIAPTIGRVAVGKVRPSNVQAWVKDRSAVLAPTTLRVVYRYLSAIFASAVRDRLIARTPCVDVKLPGVDRDKRVIPTPEQVHSLARLMPDRLSAAVYVAAGCGLRLGEVLGLEVEDIDFDRAELSVRQQLKSHKGRPPYLGRPKTKTSSRVVELPEVVAHALRSHLENAPKPIEVDDDTNPRKPIRRPAALVFRSADGGPVFASTFSRTWVPVRTQVGLPGRWGFHGLRHYYATLLIHAGASVKTVQLALGHSTPTITLNEYVHEWPDVLDRTRALVDGALGKPETAATPAVSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTQ1</accession>
    <name>U5VTQ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cadmium resistance transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09775"/>
    <dbReference type="EnsemblBacteria" id="AGZ40244">
        <property type="protein sequence ID" value="AGZ40244"/>
        <property type="gene ID" value="AFR_09775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1993"/>
    <dbReference type="eggNOG" id="COG4300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071117_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004676">
        <property type="entry name" value="Cd-R_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03596">
        <property type="entry name" value="Cad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="19867" checksum="7C0112878FED6D11" modified="2014-01-22" version="1">MVQTMLAAAGVFAGTNVDDIIVLTVLFLSARAAGRPRVWQIWLGQYAGIAVLVLVSALAALGLTLIPDRWVPLLGLVPIALGVRGLIGAIRRRDGDEEQPPAATNALAVAGVTIANGADNLAVYTPMFRTLGVGDTVVTVIVFAVLVAVWCAAASWLGSHRRVIALAERWGQWLVPLVFITIGVVILLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU72</accession>
    <name>U5VU72_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_04435</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358144.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04435"/>
    <dbReference type="EnsemblBacteria" id="AGZ39176">
        <property type="protein sequence ID" value="AGZ39176"/>
        <property type="gene ID" value="AFR_04435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.897"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="26718" checksum="2D0DCDE0FAD28AA0" modified="2014-01-22" version="1">MRFDQVWFRYARREDWTLRMVDLTVEPGATVVVLGRNGAGKSTLLQLAAGVLRPVRGAVRQRPGVVGWVPERFPADQPFTTEQYLTRTAAVRGLRGGREVDHWITRLGLDDHRHTRLAALSKGTAQKVGLAQALLVSPGLLVLDEPWEGLDSHARTLIPQIVGEVTAAGGAVLVSDHRGEIAGLPDAIRWTVTDGTVKTERAEPGDPGEVVVEIAVRRTDADTTMARLRADGHRVLGVRTERPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU83</accession>
    <name>U5VU83_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dihydroxy-acid dehydratase IlvD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IlvD/Edd family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360307.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10725"/>
    <dbReference type="EnsemblBacteria" id="AGZ40434">
        <property type="protein sequence ID" value="AGZ40434"/>
        <property type="gene ID" value="AFR_10725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2186"/>
    <dbReference type="eggNOG" id="COG0129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_3_1_11"/>
    <dbReference type="OrthoDB" id="193579at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="572" mass="60551" checksum="8E5AF3BC35495049" modified="2014-01-22" version="1">MPELRSAEWYAGQDRNAYIHRAWMRRGSPNSAFEGRRPQIAIANTASDLTPCNSHLDEVAEHVKRGIWEAGGVPLNLPCVSIGETQVRPTAMLWRNMAAMATEEMLRANPIDGVVLLGGCDKTIPSLLMAAASVDLPAVVVPGGPMLTGTFRGVPLGCGTDVWRLSEEVRAGTLSEAAFLDSESSMIRSRGHCNTMGTASTMGLLAEALGAVLPGTAGTPAPDSRLLARSHETGRLIVEMVAAERRPSQVLTTGSFHNAIVALAALGGSTNAVVHLLAIAGRLGVPLSLADFDRIAAEVPLLVNLQPAGKYLMEDLYRAGGLAAVLREVADLLDQDAIDVTGRPFVESLTEAQIWDQDVIRTRDKPLQPNAGIAVLHGNLAPKGAIIKPAAASPHLLQHRGRALVFDSVEDFNARIDAPGLDVDADTVLVLRGCGPRGYPGMPEVANMPLPTKLLHEGVRDMVRICDGRMSGTAYGTVVLHVAPEAAAGGPLDRVRDGDWITLDVAGRSITVDVPDEEFAAREPSAALQAALARPDRGWQKLYVDTVLGADTGADLDFLVGGSGDTVSRESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUB9</accession>
    <name>U5VUB9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transketolase</fullName>
            <ecNumber evidence="5">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_10640</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10640"/>
    <dbReference type="EnsemblBacteria" id="AGZ40417">
        <property type="protein sequence ID" value="AGZ40417"/>
        <property type="gene ID" value="AFR_10640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2169"/>
    <dbReference type="eggNOG" id="COG0021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005478">
        <property type="entry name" value="Transketolase_bac-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033247">
        <property type="entry name" value="Transketolase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522">
        <property type="entry name" value="PTHR43522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00232">
        <property type="entry name" value="tktlase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="10">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ40417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="702" mass="75790" checksum="075CA10296669439" modified="2014-01-22" version="1">MNWSDLDRRAVDTVRVLAMDAVEKSGNGHPGTAMSLAPAAYLLFNRVMRHDPTDPLWAGRDRFVLSCGHSSLTLYIQLYLSGYGLALDDLASLRQWGSLTPGHPEYGHTPGVEVTTGPLGQGIGNAVGMAMAARRERGLFDPTADGDSLFDHNVYALCSDGDIEEGVSHESSAIAGVQKLGNLTLIYDDNEISIEDDTRIAKNEDVGARYAAYGWDVRHVDWRKGDPEKGSEYHEDVEALYAAIRAGQEVTDKPTFIVLRTIIGWPAPNKQNTGKIHGSALGADEVKATKEILGFDPNKTFDVAEDVLAHTREVVQRGRAAHEEWSKAFEGWAAANAEGKALFDRLSTRTLPEGWHKALPEFPADEKGVATRAASGKVLDALAPVLPELWGGSADLAESNNTTMKGEPSFIPSEYATKAFPGHEYGRTLHFGIREHGMGSILNGIVAHGGTRPYGGTFLVFSDYMRGAVRLSALMKLPVTYVWTHDSIGLGEDGPTHQPIETLSALRAIVGLDVIRPADANETAWAWRGALEKTDRPAALALSRQNLPTIDRTKYASAEGTLKGGYIVSEATGGEPQVILIGTGSELSICLTAQERLEAEGTPTRVVSMPCQEWFYEQDTAYQQTVLPRGVKARVTVEAGIRMSWDRLLGEAGEAVSIEHYGASAPAKVLFEQFGFTADNVVAKANAALAKVGEITGHTTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUY7</accession>
    <name>U5VUY7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360728.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11900"/>
    <dbReference type="EnsemblBacteria" id="AGZ40669">
        <property type="protein sequence ID" value="AGZ40669"/>
        <property type="gene ID" value="AFR_11900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2423"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_3_4_11"/>
    <dbReference type="OrthoDB" id="1864829at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="4"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="11068" checksum="8AFDA3D2DCDB7DB3" modified="2014-01-22" version="1">MALFTARTSAEGGRVVVALTGECDLAGRDEMETVLLTAVESGKPVRVDLTGVTFLDSSGIHTLVTAYHAARRTGGRLTIANARGSVATVLDMTGVATLLSPDEPGHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV04</accession>
    <name>U5VV04_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11665"/>
    <dbReference type="EnsemblBacteria" id="AGZ40622">
        <property type="protein sequence ID" value="AGZ40622"/>
        <property type="gene ID" value="AFR_11665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2376"/>
    <dbReference type="eggNOG" id="ENOG5031NBJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156349_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="15469" checksum="80DB652B10F5EB2C" modified="2014-01-22" version="1">MPRKPPKRLRRPDHGPAPQPQIDGLSARTHAAIRYVEDVHYWPGAVARALAGYQYLLREPGRWLPGDPVISPGLEPEADRSALQDMMAKLPPEPRRELELLLAPLDDDYMRRTLPEPGENPTPDQRWRWRDREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVB9</accession>
    <name>U5VVB9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flp pilus assembly protein CpaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359181.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06805"/>
    <dbReference type="EnsemblBacteria" id="AGZ39650">
        <property type="protein sequence ID" value="AGZ39650"/>
        <property type="gene ID" value="AFR_06805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1390"/>
    <dbReference type="eggNOG" id="COG3745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092374_1_0_11"/>
    <dbReference type="OrthoDB" id="2028007at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031571">
        <property type="entry name" value="RcpC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16976">
        <property type="entry name" value="RcpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RcpC" evidence="1">
        <location>
            <begin position="116"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="25819" checksum="CA6682554EE4A29F" modified="2014-01-22" version="1">MRRRILIVLAALVLAGLSGTALLLYARGVDQRAVDGRKAVSVLLAEERIPAGTTAAQIREKKLARTVVMPADSLPDDTVGSLDKSLDGLALSADLQPDQLLLRGQFSRPDTDSSAAVGVPDGDLGVSVEVTMAPGVAEKVAAGDKVTVFVTYPKDARPSDQKTRILLPDATVISITTGPPSDVTPAPTSTRNNRAVAQSYPATLAVDQDDAVRLVHAAQTGSIYLGLLGETTKVSPSPGVDYDSLWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVI2</accession>
    <name>U5VVI2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360876.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12655"/>
    <dbReference type="EnsemblBacteria" id="AGZ40817">
        <property type="protein sequence ID" value="AGZ40817"/>
        <property type="gene ID" value="AFR_12655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2570"/>
    <dbReference type="eggNOG" id="COG2764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_17_2_11"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06588">
        <property type="entry name" value="PhnB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028973">
        <property type="entry name" value="PhnB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16107" checksum="C2414B3D32D529DD" modified="2014-01-22" version="1">MALTTTTHLNFRGDARQALEFYQSVFGGHLTVVAYGDFGMPKELPDADKVVFGQVSAENGFSIAAYDAPSQSPAAPAPTATTRENGMTLTGERVFVAVGGDTAEEISALWDKLSADAEIVEKFGPSAFSPGFGMLTDQFGVTWVLQVNAAHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVR8</accession>
    <name>U5VVR8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glucose-methanol-choline oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_13105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13105"/>
    <dbReference type="EnsemblBacteria" id="AGZ40907">
        <property type="protein sequence ID" value="AGZ40907"/>
        <property type="gene ID" value="AFR_13105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2661"/>
    <dbReference type="eggNOG" id="COG2303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002865_7_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="PTHR11552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMC_OxRdtase_N" evidence="3">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="38879" checksum="6DB9BD175D2C2CC0" modified="2014-01-22" version="1">MQQGISAFEDLNGSGHERVAYAPLSVAGGTRMSAARAYLHRATRRPNLRLELHCEVRRIIFDGTTATGVEVMTPDGEARFTARREVIVCAGTMESPLLLERSGVGDPDVLAAGGVPMVAANPAVGENLRQHRGAVFALRLRGVPGRNRELVTPAARLRTTARYLLRRSGALAHGGTTVLAVLRSSPAVAGPDMEVLFTPVSVSGHDGTSAGADAGDGVTVGFYPHAPTSTGSIHLTGPSAHHPPRLVPGYLSTGHDRDLTVAAFRRVRQILEADPFASAASETAPGPQVSDDDSVLRYVLEHGFAGNHQVGTCALGPEGAVDDDLRVRGTQRLRVADASVMPTLTTGNTVAPSMAIGWIAGDLIRHGKAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVS0</accession>
    <name>U5VVS0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359272.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07260"/>
    <dbReference type="EnsemblBacteria" id="AGZ39741">
        <property type="protein sequence ID" value="AGZ39741"/>
        <property type="gene ID" value="AFR_07260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1482"/>
    <dbReference type="eggNOG" id="ENOG5032T13">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110680_0_0_11"/>
    <dbReference type="OrthoDB" id="1968131at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="15428" checksum="D6491D3FADEF153C" modified="2014-01-22" version="1">MGYTLEAIIGPAADVRRAVAGQPDAVIVPLHEDLVLVPMTEKLFAAFAGPEGGRAVKFETPGTVAYVEAEFFGGIGTQRAEIWTDGTLTFGPLEMDEGETVEVTPISQVLAKLGVKRKDRRDEFDVVELDRHRGTEGWLSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVW6</accession>
    <name>U5VVW6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359322.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07510"/>
    <dbReference type="EnsemblBacteria" id="AGZ39791">
        <property type="protein sequence ID" value="AGZ39791"/>
        <property type="gene ID" value="AFR_07510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1533"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_4_1_11"/>
    <dbReference type="OrthoDB" id="1135906at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="31604" checksum="E6A4471E2F8ACA91" modified="2014-01-22" version="1">MDQYTAQDPRSQHPGPALRGGDQTHPGKTNKMPDRPDHGEETYRGSGRLTGKRAVITGGDSGIGRAVAIAYAREGADVLLSYLPEEEDDALDTAQLIEKAGRTAVRVPGDIRDRAQCEEIIRRAVDDLGGLDILVNNAAFQMAQPGGITDITDEQFDRVMKTNLYAMFWLCRAAVPHLRPGATIINTASIQAYQSSSALLDYATTKGGIVAFTKALAEDLAEQGIRVNAVAPGPIWTPLIPATMTDEKVEKFGSDTPLGRAGQPSELAPAYVYFASQESSYTTGEVLGVTGGKPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW13</accession>
    <name>U5VW13_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpsT</name>
        <name evidence="9" type="ORF">AFR_07785</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds directly to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359377.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07785"/>
    <dbReference type="EnsemblBacteria" id="AGZ39846">
        <property type="protein sequence ID" value="AGZ39846"/>
        <property type="gene ID" value="AFR_07785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1589"/>
    <dbReference type="eggNOG" id="COG0268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160655_0_1_11"/>
    <dbReference type="OrthoDB" id="2005443at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_S20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_S20_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="PTHR33398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="SSF46992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="88" mass="9639" checksum="1CB2DD6385008A09" modified="2014-01-22" version="1">MANIKSQIKRNRQNEKARLRNKSVKSSLKTVIRKLNEASEAGNNESATALLRDASRQLDKAVSKGVIHKNQAANRKSAIAKRVASLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWC5</accession>
    <name>U5VWC5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361362.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15105"/>
    <dbReference type="EnsemblBacteria" id="AGZ41303">
        <property type="protein sequence ID" value="AGZ41303"/>
        <property type="gene ID" value="AFR_15105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3067"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_1_11"/>
    <dbReference type="OrthoDB" id="1054546at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="13"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="325" mass="35092" checksum="9F9CFED61CB1B79D" modified="2014-01-22" version="1">MRTAKLGDLEVGRIGLGTMGMSHGYTGAGSDDAESIRTIHRAIDLGVTLIDTAEVYGPFVNEELVGKALAGRRDQVVLATKFGLVSHTGAGNADSSPDNIRTAVEGSLRRLGTDHIDLYYQHRVDPKTPIEDTMGVLAELVQQGKVRHIGLSEAWIDTVRRAHAVHPVTALQSEFSLWTRDQEEMLPVLRELGIGLVAYSPLGRGFLTGALRSVADLDALDDSDFRKNNPRFTGENFERNLRLVDGVRAVADQAGATVGQVALAWLLAQGDDIVPIPGTKRVSRVEENVGADAVELTPEQVATLTALPAPEGGHHTEDQMQLIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWH0</accession>
    <name>U5VWH0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase GCN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361186.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14225"/>
    <dbReference type="EnsemblBacteria" id="AGZ41127">
        <property type="protein sequence ID" value="AGZ41127"/>
        <property type="gene ID" value="AFR_14225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2886"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_21_4_11"/>
    <dbReference type="OrthoDB" id="1390922at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16264" checksum="D277879BD5287707" modified="2014-01-22" version="1">MGSTNVTIRSYAEGDWAAIAAIHDAARLDELRDSVGVEAFLTLAQTAEGEGLFDGQLWVADVGGSVAGFVAVEDDEVTWLYVDPQRYRQGVGRALLRHALAAAGPRVEVTVLDGNTAALGLYLSEGFTITETRTGSLVGNEAFTATGHIMHRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWJ1</accession>
    <name>U5VWJ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361293.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14760"/>
    <dbReference type="EnsemblBacteria" id="AGZ41234">
        <property type="protein sequence ID" value="AGZ41234"/>
        <property type="gene ID" value="AFR_14760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2997"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_3_11"/>
    <dbReference type="OrthoDB" id="1617768at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="113" mass="12737" checksum="C2C02F5CF69B3ABA" modified="2014-01-22" version="1">MARAATTSDAFNAIAEPQRRDILTLLRAGDRPVTDLAQQLGMSLPGASKHLRVLREVGLVRVRGAGRQRLYGLDARGLRPIHEWVGGFEEFWTGSFDRLDSYVQDLKQTRMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWL0</accession>
    <name>U5VWL0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361452.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15555"/>
    <dbReference type="EnsemblBacteria" id="AGZ41393">
        <property type="protein sequence ID" value="AGZ41393"/>
        <property type="gene ID" value="AFR_15555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3159"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051479_3_0_11"/>
    <dbReference type="OrthoDB" id="1079953at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31617" checksum="62C8E364731F55A1" modified="2014-01-22" version="1">MSWVEEFRAHEAIYRRAPSAHNTQPWTLEYRADEIVIGIDPARALPGSDPGGRDLRLGLGAFVETCLIVAADAGLSVTYDSRRLLAAPSPYVTPFTAADVSARRVARGAYRPGVVSTAGLEPGLVHVPSRDLADDLAVADRWMFSTPRVALELRDWLRLDPGDPRYRLDGLTDRALELSRGEARVLASALRAYPVTRRLGLAALLAASGRNLLRYDGSVLVLTGDVSLDAGRRLLRTWLTLGRSGLAVHPLSQLLDCPATAARLAARVGATPLAVFRVGVPLHEPVRSARRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWN0</accession>
    <name>U5VWN0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HDOD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361338.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14985"/>
    <dbReference type="EnsemblBacteria" id="AGZ41279">
        <property type="protein sequence ID" value="AGZ41279"/>
        <property type="gene ID" value="AFR_14985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3043"/>
    <dbReference type="eggNOG" id="COG3434">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044951_0_0_11"/>
    <dbReference type="OrthoDB" id="753726at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014408">
        <property type="entry name" value="dGMP_Pdiesterase_EAL/HD-GYP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013976">
        <property type="entry name" value="HDOD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08668">
        <property type="entry name" value="HDOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003180">
        <property type="entry name" value="DiGMPpdiest_YuxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51833">
        <property type="entry name" value="HDOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDOD" evidence="1">
        <location>
            <begin position="196"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="410" mass="43480" checksum="ABD5CAE2B941DCBD" modified="2014-01-22" version="1">MDPRAVEQPTVHVGRQVIFDQYGEVFACELLFRGAPAQVTSLFVRTFTDFGLPELVGNRPCLVTVPRDFLVGDLPMPFDPGQVILEVVVAADLDDAVLTGIEALAAGGFTIAVTAEVADFSDRLLPVAAYLKINLPGAAESVMDLARTCHERHPHLRLIAAQLETEAQKHRAPALGFSLFQGSVLGRPHVVSMAELSPARLSRLRLVSVLAAAEVDFDEAVSLIPGDPALTYRLLQASNAAASGLPARVSSVREAAVLLGLPTIRHWVTLMLLSDLSKAREEQLIPTMIRARMCQSVAEQAGLPGDAAFCVGLLAGIAELIGQSRPALAQQLPLTDEVNDALADGAGPLGHLLAAVRDYETGDPSGLAELVDPGAAVHAYLAATAWCNQVMGAAGPVERQTSARRPRIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWT4</accession>
    <name>U5VWT4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15260"/>
    <dbReference type="EnsemblBacteria" id="AGZ41334">
        <property type="protein sequence ID" value="AGZ41334"/>
        <property type="gene ID" value="AFR_15260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3099"/>
    <dbReference type="HOGENOM" id="CLU_2393226_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="93" mass="9684" checksum="C4128A8512EA557A" modified="2014-01-22" version="1">MTDDDRAPHAAQGAAAMETRPLPAPAVDLSVFVLATVDDHIAAFERIRGHLSAGRPIGAGHRLTLAADSVALAAQYVSRMSSALELPPADRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX08</accession>
    <name>U5VX08_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome bc1 complex cytochrome b subunit</fullName>
            <ecNumber evidence="2">7.1.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cytochrome bc1 reductase complex subunit QcrB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_09435</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359797.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09435"/>
    <dbReference type="EnsemblBacteria" id="AGZ40176">
        <property type="protein sequence ID" value="AGZ40176"/>
        <property type="gene ID" value="AFR_09435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1923"/>
    <dbReference type="eggNOG" id="COG1290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031114_2_0_11"/>
    <dbReference type="OrthoDB" id="262439at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:ubiquinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13631">
        <property type="entry name" value="Cytochrom_B_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="CYTB_NTER" evidence="9">
        <location>
            <begin position="15"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="517"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="527"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="541" mass="59632" checksum="F0E5AD2EED00BE20" modified="2014-01-22" version="1">MKRRKLDLAQVPANVGKGLDDRYKAATPLRGLLNKVFPDHWSFLLGEIALFSFIVLLLTGVFLTLFYDPSMREIAYEGAYPALRGTEMSAAYASSLHLSFEVRGGLFMRQMHHWAALLFMASIVVHMARIFFTGAFRKPREANWAIGVALFLLGFLAGFTGYSLPDDGLSGTGLRIASAIMLSIPVVGTWVSASVFGGEFPGEFIIGRFYIAHVLLIPGGLLALISVHLGLVFAQKHTQWPGPGRTNDNVVGERMFPRYALKQGGFFMAVFGVIALMAGLFQINPIWLFGPYRASEVSSASQPDWYVMFMDGLVRLMPAWQIDIPIGDGYVIPPLFWPAVVGLGALTTVPMFYPFIEARRLKDKGSHHLLQRPRDAPERTGLGAMAFTFFIVATISGGNDVIADKFHISLNAMTWAGRIGLVILPPLAYWISVRICLGLQQHDREVLAHGVETGIIKRSPDGRFYEVHQPLGPVDDHGHPLPLDYAGWVVPKKMNRLGALAPAVKGFFYPVEKPAEAPVSPGMPPITAREEREEITSGQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXD2</accession>
    <name>U5VXD2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1737 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16260"/>
    <dbReference type="EnsemblBacteria" id="AGZ41534">
        <property type="protein sequence ID" value="AGZ41534"/>
        <property type="gene ID" value="AFR_16260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3299"/>
    <dbReference type="eggNOG" id="COG5515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186571_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013619">
        <property type="entry name" value="DUF1737"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08410">
        <property type="entry name" value="DUF1737"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1737" evidence="1">
        <location>
            <begin position="9"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="58" mass="6321" checksum="B8994FA1AD9BAE44" modified="2014-01-22" version="1">MSDPTERLRYRLITGPDDAEFCARVSKLLDSGYRLHGSPALTFDGERVIAAQALVLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXS5</accession>
    <name>U5VXS5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydroxyacetone kinase subunit DhaK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361632.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16375"/>
    <dbReference type="EnsemblBacteria" id="AGZ41557">
        <property type="protein sequence ID" value="AGZ41557"/>
        <property type="gene ID" value="AFR_16375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3321"/>
    <dbReference type="eggNOG" id="COG2376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017054_0_0_11"/>
    <dbReference type="OrthoDB" id="857242at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012736">
        <property type="entry name" value="DhaK_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004006">
        <property type="entry name" value="DhaK_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02733">
        <property type="entry name" value="Dak1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02363">
        <property type="entry name" value="dhaK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51481">
        <property type="entry name" value="DHAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaK" evidence="1">
        <location>
            <begin position="7"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="34093" checksum="5606AACA5F29777C" modified="2014-01-22" version="1">MNKLINEPANVVSDALRGMAEAHPELRVDHENRIVYRAGAPVRGKVGLISGGGSGHEPLHAGFVGPGMLDAACAGEIFTSPVPDQMAAATTAVDGGAGVLHIVKNYTGDVMNFEMAAELAAAEASVEVVAVVTDDDVAVQDSLYTAGRRGVGVTVLVEKIAGAAAEQGRSLAEVADVARKVNANGRSMGVALTSCTVPAAGKPTFDLPDGQMEVGIGIHGEPGRRRVPIAPAAEVADLLLGPILADLDFTGGDGVIAFVNGMGATPQIELYVMFHEVAAILAKAGITVARTLVGSYITSLDMAGCSVTLLKADDDLLRLWDAPVRTPALRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXX1</accession>
    <name>U5VXX1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16695"/>
    <dbReference type="EnsemblBacteria" id="AGZ41617">
        <property type="protein sequence ID" value="AGZ41617"/>
        <property type="gene ID" value="AFR_16695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3385"/>
    <dbReference type="eggNOG" id="COG2230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037315_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="13"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="221" mass="23887" checksum="862157583FBF3262" modified="2014-01-22" version="1">MLRRTGREPSARILDLGCGEGAWVLQALTHFPDGHADGVDLDPYGLERAQAAAGERGLADRLTVHERDARTYVADGDYDLVLCVGSTHAFGGFAETLELAGQHVNPHGVLLVGECFWSVPPTAAALEALDAKADDYTDLAGLADVAARAGWAPVYAHVSDLAEWDDYEWSWTGSLTEWALDNPGHPDAADALAVAQEHREQWLRGYRGVLGFVTLVLRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY25</accession>
    <name>U5VY25_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18125"/>
    <dbReference type="EnsemblBacteria" id="AGZ41903">
        <property type="protein sequence ID" value="AGZ41903"/>
        <property type="gene ID" value="AFR_18125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3679"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_28_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011075">
        <property type="entry name" value="TetR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16925">
        <property type="entry name" value="TetR_C_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="22911" checksum="55069265DCC97B34" modified="2014-01-22" version="1">MVTAPPEPRKLTRKGQATRDRILQTAVDLIAARGVAGTSTEDVRKAAGISGSQLYHYFDSKQALIRAVIARQADAVPIPGRPMMGALDSLEALRAWADAGIERQETIGFGGECTLGTLAGELSAGDEASREQIGQGFLRWQELLHTGLQAMQDRGELSPDADLDELALALLTALQGGTLLTQTLRNTKALRAAMNAALSYIASYSTPNPDRPPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY93</accession>
    <name>U5VY93_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360618.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11585"/>
    <dbReference type="EnsemblBacteria" id="AGZ40606">
        <property type="protein sequence ID" value="AGZ40606"/>
        <property type="gene ID" value="AFR_11585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2361"/>
    <dbReference type="eggNOG" id="ENOG5031MNQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075797_0_1_11"/>
    <dbReference type="OrthoDB" id="1899949at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016566">
        <property type="entry name" value="UCP010219"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11361">
        <property type="entry name" value="DUF3159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="23281" checksum="53E31D844A82DE6B" modified="2014-01-22" version="1">MAQPESLADLLGGRRAALDATVPPVVFVAAWLIGGRSLWIGAAAAIVAAVGVAGWRLRAGDKPRAVLIGLLGVCVAALVALRTGRAEDFFLLQIVTNAASALLWAVSIVVRWPLLGVIVGTVLGQKTRWRRDPALLRAYCLGSWVWVAQYLVRLAVFVPLWAAHAVVALGIARGVLTWPLVAACLAVSWWVIRRSLPPEHPGLRHPQSAVDAHADRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYZ6</accession>
    <name>U5VYZ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361014.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40955">
        <property type="protein sequence ID" value="AGZ40955"/>
        <property type="gene ID" value="AFR_13345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2710"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_0_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="32"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="30368" checksum="9AA4DAB3613C4346" modified="2014-01-22" version="1">MSGDEGTNRLGDYLRARRELVTPEQAGIQPGARRKVKGLRREEVALLAGISADYYLRLERGRDHNPSPQVLEALARVLHLDDVERLYLLELAAPRPRNRPRRRPEQVPARLHHLLAAVGVPAFVEGRSFDVLAANTLAAALSPRLRPGQNRLRSMLLDPEEREFHVDWERSVASFVGAFRRTVGDASDDARIVELVGELSLSSARFRSLWARHDVRELQGGTTTVRHPVVGELTLHRDKLPVDGLLLVLYYPDEHGDSADKLRLLSSLTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ75</accession>
    <name>U5VZ75_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F420-dependent oxidoreductase, g6pdh family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20080"/>
    <dbReference type="EnsemblBacteria" id="AGZ42288">
        <property type="protein sequence ID" value="AGZ42288"/>
        <property type="gene ID" value="AFR_20080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4073"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019945">
        <property type="entry name" value="F420_G6P_DH-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03557">
        <property type="entry name" value="F420_G6P_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="34881" checksum="27A8D694DBD55A3F" modified="2014-01-22" version="1">MIMKFGYKLAAEGFGPKELIRQAVRAEEAGFDFVEISDHYHPWLDVQGHSPFAWAVLGAIAARTQRLGLATGVTCPTVRYHPAIIAQAAATLAIVSDDRFTLGVGAGERLNEHVVGQGFPSVRGRHERLKEALEIIKLLWQGGYQSYEGKHLQLEDARVFDLPDKLPVIAVAAGGKNAATMAAELGDGLFATEPRSDLVETYQGAGGSGPRYAEVPMAWARTEEDGVQEALKTTRWAVTGWKVMSELPNPVNFDAASQTVRAEDIREKFATGPDPEPYVEAVRKYTDAGFDHIVLQNAGPDPDGFLDFFHKELHDRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZA9</accession>
    <name>U5VZA9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362465.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19100"/>
    <dbReference type="EnsemblBacteria" id="AGZ42092">
        <property type="protein sequence ID" value="AGZ42092"/>
        <property type="gene ID" value="AFR_19100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3879"/>
    <dbReference type="HOGENOM" id="CLU_1860909_0_0_11"/>
    <dbReference type="OrthoDB" id="2214067at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="14731" checksum="774E772814A31977" modified="2014-01-22" version="1">MSGRALTIFVVALLSVLGLVFGITGDGQDEVVADGPLIDAEVIEHRGFGDDANVVLRVSDPRTTLLCSIWRDDFDGDWPPVGTVLAVEYTAPYCALPIVHKGTPKAELIIGGSLGLLGLAAHFLWSTRRRRVKSFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZG3</accession>
    <name>U5VZG3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Keto acyl reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362651.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20035"/>
    <dbReference type="EnsemblBacteria" id="AGZ42279">
        <property type="protein sequence ID" value="AGZ42279"/>
        <property type="gene ID" value="AFR_20035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4065"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_0_11"/>
    <dbReference type="OrthoDB" id="968232at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="25228" checksum="F3042227A12E2A0E" modified="2014-01-22" version="1">MSEKTVALITGANKGIGYEIAAGLGALGWAVGVGARDDGRREAAVEKLRAGGADAFGVPLDVTDDNSVTAAARLIEERGGRLDVLVNNAGITGGRPQEPSRVDPAIIRTVVETNVIGVVRVTNAMLPLLRRSASPRIVNMSSSVGSLARQSGVGRELTTGPIAVAYSPSKTFLNAVTIQYAREFQGTNILINAACPGFVATDLNGFRGVRTPEQGAATAIRLATLPDDGPTGGFFEDEGEVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZU7</accession>
    <name>U5VZU7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361230.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14445"/>
    <dbReference type="EnsemblBacteria" id="AGZ41171">
        <property type="protein sequence ID" value="AGZ41171"/>
        <property type="gene ID" value="AFR_14445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2932"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082109_0_0_11"/>
    <dbReference type="OrthoDB" id="1517743at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="147"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="27505" checksum="F08C743BE8783FE4" modified="2014-01-22" version="1">MAVTLALLAALSYGVGDFIGGVGGRRSNPALMPIAVQMVGVVTAVVAVLVIGGTPTPHVWVWGALSGVGSGIGNVCLLRGLAVGRMSVVAPLSAIITAALPAVVGLLAGDRLTWWGWTGIVLALPAIALASRSGSGQGYRMTDVGYGLAAGLGFGLLFIALDQAGTDAGAWPLLPGQLVALVLVLVVALPQLSQASDIGAVVRWGGASGLLGGCANLLFLFATGAGQLTITAVLAGLYPAVTVTLAAIVLHERINRSQQYGLLAAAASIVLIITGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZV2</accession>
    <name>U5VZV2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative chorismate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21205"/>
    <dbReference type="EnsemblBacteria" id="AGZ42513">
        <property type="protein sequence ID" value="AGZ42513"/>
        <property type="gene ID" value="AFR_21205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4300"/>
    <dbReference type="eggNOG" id="COG0147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate_bind" evidence="2">
        <location>
            <begin position="34"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30755" checksum="7880B7C356DF2586" modified="2014-01-22" version="1">MGACAIPPDDSGGFAPGAGPEQWRIGPWRDSWSRSEHAAAVSAAREAIGRGDIYQVNVVGHSSAPYTGDPRAALNRVAGLPGARYGGVLSGSGWALATASPETLVEVRDGRVVTRPIKGTRPATARGRRELLASAKERAEHIMIVDLERNDLARLDAVGPVRVDELFGVRRWCELWQAESVVSAPIEGRLDLADLLRAMCPPGSVTGAPKRSALAEIARLEPVGRGAGMGALGWVGTDHVDLGVSIRTAAVDGDRVHLWAGGGITWDSDPYAEVDEAAAKAAPMRAALSGVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0B5</accession>
    <name>U5W0B5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21025</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362849.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21025"/>
    <dbReference type="EnsemblBacteria" id="AGZ42477">
        <property type="protein sequence ID" value="AGZ42477"/>
        <property type="gene ID" value="AFR_21025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4262"/>
    <dbReference type="eggNOG" id="COG0297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_3_11"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009250">
        <property type="term" value="P:glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011875">
        <property type="entry name" value="M1P_synthase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02149">
        <property type="entry name" value="glgA_Coryne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="27"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="202"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="407" mass="43800" checksum="C7D598BBE2478F76" modified="2014-01-22" version="1">MTDSRSKSHGTDLRVDLLTREYPPEVYGGAGVHLEYLARDLRPLADVRVHCFGAERDEPGVTAYPEPAELAGANAALRTMGVNLAMAAGCEGTDIVHSHTWYANFAGHTAKLLHGVPHVVTTHSLEPLRPWKAEQLGGGYALSSFCERTAIESADAVIAVSGGMRRDVLAAYPSVDPDKVSVVYNGIDTEQYAPDRGTDVIERLGIDLNRPSVVFVGRITRQKGLPYLLRACHDLPPETQIVLLAGAPDTKEIAAEVEGLAIQLRNARDPQGVIWVQEMLPKHEVVQVLTHATVFVCPSVYEPMGIVNLEAMACETAVVATATGGIPEVVADGETGVLVPIEQVTDGTGTPVDPDRFVADLATAMSKLIKDPARAAEMGLAGRRRAVEKFSWSRIAVDTLEVYRSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0K1</accession>
    <name>U5W0K1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerophosphoryl diester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16165"/>
    <dbReference type="EnsemblBacteria" id="AGZ41515">
        <property type="protein sequence ID" value="AGZ41515"/>
        <property type="gene ID" value="AFR_16165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3280"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_470638_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08577">
        <property type="entry name" value="PI-PLCc_GDPD_SF_unchar3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039559">
        <property type="entry name" value="AIM6_PI-PLC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="20"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="579" mass="63202" checksum="C7FE734419FE38F8" modified="2014-01-22" version="1">MAAGLALAAPVPASAGHRSFDLQAHRGGLGLRPESTLSAFTNALHVGVSTLELDVQITEDGRPVVTHDRKVDGRKCLDTAPVSAGDPEFPYVGKYVNTLTLAQVKTLDCGTRTLPDFPEQQADPGARMPVLGEVFDLVKRFRADGVKLNVETKVEAGAPSETAPREQFVQVVAREVRAAGFLRRVTIQSFDWGSLMRMRQVEPRLPVVALTNIDFLQTGQPGASPWLGGLDIDDFGGDPIAAIKSFGATTFSPVHGTPQNGKVGDPGYQPYVTKALVARAHRAGIKVVPWTVDDPATMNKLISDGIDGLITDYPDRLRQVLADRGFRLPPPYAVLARPLEQAHAHNDYEHTNPLFDAWEHGFTSVEADVYLVGGELLVGHDPEDVVPGRTLQKLYLDPLLAHATARGDRIPPMQLLVDLKNTGAATYTELDQVLRGYRRILTRYAHGRVTEGAVTVVISGDRPRDLMAAQPVRYAFYDGRLADLGGAAKASFMPLISDNWTNTFTWTGVGPMPAAERTRLRDLVATAHQHRQRVRFWATPDAPGPEREAVWRELVAAGVDHLNTDDLAGLQSFLSRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W108</accession>
    <name>U5W108_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23365"/>
    <dbReference type="EnsemblBacteria" id="AGZ42943">
        <property type="protein sequence ID" value="AGZ42943"/>
        <property type="gene ID" value="AFR_23365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4735"/>
    <dbReference type="eggNOG" id="ENOG5033QSY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110206_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665970" evidence="1">
        <location>
            <begin position="22"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="21007" checksum="9C9D5A8CDA8DE3AD" modified="2014-01-22" version="1">MASAACLVLTPAAAVATPARPAARPAECATPSIDRLQQWLASGEGTTVPATGSLLLPNRHGDRYTAKVDFVNAEWHVVVVWLANEFEAQVDLADSKGFWLTYSATEDLYVQLRPASHWSGGAQWATAVPSTQGRTVTRFFSFRPQAWGAVPELGAPAHSFASALSEARGLVFVGKTANELSFRGLRIHRYTPPCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1G4</accession>
    <name>U5W1G4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Fibronectin type III domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_22960</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363373.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22960"/>
    <dbReference type="EnsemblBacteria" id="AGZ42862">
        <property type="protein sequence ID" value="AGZ42862"/>
        <property type="gene ID" value="AFR_22960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4653"/>
    <dbReference type="eggNOG" id="COG4733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_224365_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="37"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665967" evidence="7">
        <location>
            <begin position="50"/>
            <end position="4050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="4025"/>
            <end position="4045"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="4">
        <location>
            <begin position="241"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="385"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="482"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="575"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="670"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="766"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="861"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="960"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1052"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1148"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1245"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1331"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1422"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1507"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1596"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1684"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1775"/>
            <end position="1859"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1860"/>
            <end position="1947"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="1948"/>
            <end position="2039"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="2125"/>
            <end position="2215"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="2216"/>
            <end position="2309"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="2310"/>
            <end position="2395"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="2396"/>
            <end position="2486"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="2487"/>
            <end position="2571"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="2572"/>
            <end position="2661"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="2745"/>
            <end position="2839"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="2840"/>
            <end position="2922"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="2923"/>
            <end position="3010"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="3011"/>
            <end position="3101"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="3102"/>
            <end position="3195"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="3197"/>
            <end position="3277"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="3278"/>
            <end position="3367"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="3452"/>
            <end position="3540"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="3625"/>
            <end position="3715"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="3717"/>
            <end position="3810"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="3811"/>
            <end position="3898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1131"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1494"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2022"/>
            <end position="2046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2731"/>
            <end position="2783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3882"/>
            <end position="3912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1131"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2028"/>
            <end position="2046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2755"/>
            <end position="2769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="3882"/>
            <end position="3896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="4050" mass="392262" checksum="E1F4898152153B0C" modified="2014-01-22" version="1">MATALPRRVQEWAMAVRRVSGPRRSVAMAAVVALAVLSSVIGLASPAAAAGGFVLSIYGGTGAAAAPVAGPVSASPIGRPDAMTSDAAGNVYVYVRSACRVVKISPAGVLSPVAGSGTCGTGSAGDALQSALSTNVTSLAVDGSGNLFVGDSGSARIYKVTPGGTLSVFAGNGTVGATTNGSATLSSVVPDSLAVDSTGTVFVGYVGRASVLKITAAGALTRYAGTGNAGLTLPGISTLANLNKPSSLAVDTSGNLYIGDTGNNRIYLVTTGQILSAFAGNGTTGSPVPGSTSSPVPTPAAMTFDSASTLYVAESSSRQILSFTLSGNGAPSLVAGSGGTGTPNPGTATSSPLGTVTALTATAPSSLVVGDSTYNYLLRIAAPTAPDAPTGLSAVPGPSRAVLTFSPPAGNGGSAITGYEVSTNNGTSWAALTVTGSTTRTGTVTGLTNGTAYTVRVRALNAIGNGAASAATTVTPATAPDAPYGLVVLAGDAAAALSFSPPLSNGGSAITGYEVSTDTGSSWTPIATTAGLLGVRNGSVTGLTNGISYTFQVRAANVVGAGPATAGVSATPRGAPYAPTALTATTGNAQATLTFTTPPDGGNTILGYQVSTDDGATWSGLVVSTSGTTNTATLTNLSNGTVYPVRVRALNLAGAGVPSAATTVSLAALVPDAPTGLVATAGDTTIGLSFAPPASTGGSALTGYEASTDNGTTWVPITTAAGLLGVRTATLTGLTNGTAYAVRVRAVNAVGPSPAGGSAAATPRGAPYAPTGLTAVAGDRQIGVTFTTPGDGGSAILGYQVSTDNGATWSGLAVTTSGTTNTATVTGLTNGVAYTVKVRAASLLGTGAASPGVPVVLQALVPGAPTGLVALAGDTTIALSFAPPATDGGSAITGYEASTDNGTTWAPITTAAGLLGTRTATLTGLTNGTTYAVRVRAVNAVGPGPAGTSVNATPKGAPYAPTGLSAVAGNTQATVTFTTPRDGGNPILGYQVSTDDGTTWSNLAVTTSGTTNTGAVGGLTNGTTYAIRVRAVSLGGTGAASAGTAVVLPALVPAAPTALFATAGDTTISVSFAPPATNGGAVITGYEASTDNGTTWAPITTAAGLLGNRTATLTGLTNGVTYTVRVRATNAAGASATSDSTTATPRGTPYAPAGLSAVPGNGQAVLTFTTPGDGGYPILGYQVSTDDGATWTTLTVATSGTDNTGTVTGLANGTTYAIRVRARNLAGAGTPSAATAVIPATVPGAPTAVAAAAGNTQITVTFTPPAATGGRPITGYEVSTDNGTTWAALPDSGLVTGLTNGVAYAVRVRAVNAVGAGTASASTTVTPIPATPDPPTGLAAERGDRSALLTFTPPVMTGTSPISGYEVSTDDGTSWAALASDRRVTGLTNGTAYTIRVRAVNASGAGPAGAAVTVTPATLPGAPTGLTVTIGDTRAVLAFTAPGDTGGDPITGYEVSTDDGTTWAPLAANRTVTGLTNGASYTFRVRAVNSVGPGPATAGSTATPATTPGAPTGVTATPGDNRAVVTFTPPASDGGSPITGYEVSTDDGVSWDTLAADGTVTGLSNNTTYTIRVRALNAAGPGPAGAATSVTPVDGLPGAPTALVAARGDRSTTLTFTAPADPGSSAISGYEVSTDNGASWAALAPDRRVTGLANGTTYTVQVRAVNDAGAGPASGGATVTPAAAPGAPAEVRASRGNRSATLTFTAPVNDGGDPITGYQVSTDNGATWVTLAGDRTVTGLTNGTTYAIRVRAVNGVGPGTPSAPVTVTPATSSGAPTALVAVRGNGSAALSFTAPDDTGGDPVTGYQVSTDNGVTWASLAGDRVVTGLTNGVTYTIRVRAVNSSGPGAASAPALVTPATAPGMPTGLSATAGDGTATLMFRAPDDGGDQITGYEVSVDDGTTWAALAGDHIVTGLTNATTYTVRIRALNSAGTGPPSASATVTPIAGVPGAPTGLTAVRGDSSVTLTWTAPADPGSSAITGYDVSTDAGVTWTALAGDGIVTGLTNGTAYDFWVRARNAVGPGPASDSVTATPATTPQAPTALTANPGDTTVTLAFRAPVDDGGAVIIGYDVSTDDGGTWAALPGDRTITGLTNGNAYTIRVRAVNDVGAGPAGDPVTATPSQGRPGVVTGLSATAGNTTATLSFTAPADPGSSAITGYDVTIDGGVTWTPLAGNRVVTGLTNGTAYFIAVRARNSTGAGPAGAEVMVIPVTTSAAPTGLTADAGDSVATLTFTAPADTGGDPVTGYEVSTDNGTTWSPLTVSGTTTLTGTVTGLTNGTPYPVRVRALTAAGPGAPSDSVTVTPATAADAPASFAVTAGDSSLQLMFSPPASDGGNPVTGYEVSVDDGTTWAAVAGDRIVTGLTNGVTYQVRVRAVTAAGPGAATDAVTITPVGGLPGAPTGLSVTRGDRSATATFTPPADPGSSAITGYEVSTDDGWNWAPLAPSGVIPALTNGTTYVVRVRAVNTTGAGPAGDGVPVTPAAVPGPPTGLRVTAGDTTATVAFTAPADDGGAAITGYEVSTDNGVTWAALANDRRIPGLTNGTTYSVRVRAVNDVGAGPAGAAVTVTPRALPGAPTDVTATPGNASATIAFTPPADGGATITGYEVSTDNGATWRSLAANGTVPDLTNGTTYTVRIRARNSAGAGPASAAVTVTPAAVPGAPTDLTAVPGDGTATLSFTAPAAGGSAITGYDVSTDDGVTWAPLAGGVVTGLTNGDTYTVRVRARNSVGAGPASNPVTVTPATVPDAPAGLTATPGNASISVTFSPPADGGSPITGYEASSNNGTTWSPLTVSGATTRTGTLTGLDNGTTYAVRVRARNSAGAGPASANATATPTAGLPGTPGGVSVARGNASATVTFTPGGGTVTGYQVSTDDGETWRTLPADGTITGLTNGVTYAVRIRAVNNSGTSAASPSVTVTPATTPGAPTQVTAVRGDRSATLTFTPPAGDGGAPITGYETSVDGGDTWTVLGDDGVVGGLSNGTAYTIRVRARNDVGAGPAAVAPPVTPAAGPGAPTGVSAVPGDGRVTVTFTPPASDGGSTITGYEVSVDNGTTWAALAGTGIVTGLTNGTAYTIRVRALSGAGTGPASAAVTVTPATVPASPTGLSAAPGDASATVTFSPPNAGGSPITRYEISTDDGASWATLAVAGTGATRTGMVDGLTNGAEVQVRVRARNDVGPGAPSAAVAVTPVTGLPSAPGDVTVTPGNASVSIAFTPPTGGVTSYEVSIDDGVTWTTLAGNGIVAGLTNGTAYTVRIRARNATGAGPASPGYVTTPATTPAAPTALSATRGPGSATLTFTPGATGGTPVTSYETSIDGGATWQTLQAGGIVTGLTNGTAYTIAVRARNAVGAGAIATVTVTPATTPGAPTAVTAARGDASASVAFTAPASTGGAAILRYEVSVDNGTTWATLAGNRIVTGLTNGTAYAIRLRAVNEVGAGAPSATASVTPATTPGAPTAVAVSPGDASASVNFTAPASTGGAAITGYQVSTDNGTTWAALAGNRIVTGLTNGTAYLIRVRAVNEVGTGAPSAATTVTPAVALPGAPTGLTVTRGDGSATVAFTAGGGDPVSYEVSADGGVTWTALAADRVVTGLTNGTTYAIAVRAVNPAGAGPATPAVTVTPATLPGAPTLGSVVFTTTSVTITFTAPATDGGSPVTAYELSLDGGATFNPVTPTGTGTLTVTVTGLTSGTVYAVALRARNAVGAGPADSEFAASTPPAAPTQLAVTRGDASATLTFTPPDDETITGYQVSTDNGATWTDLAVEGTSPLTATVTGLDNTSTYRFRVRAVNEAGPGRASDAVTSTVATAPGAPQAVTATGRTSSILVTWAPPDNSPVPVTGYRAVASPGPATCTTGPSETSCVLGGRAGVEYTVRVVALSTGGSTTSTASATATPVAPSLPTAPPSSAPSLTTADGDITTAVPGQEIVLVGDGFAPHSTVQIAVYSKPQLLDTVVTDADGSFRKLVRLPAGLAAGQHTLLATGVDPDGNVLVRTLSVTVKAATSAPTAPSVDPEPGTSMPKTGQNIALVAALGLLMICVGTGLVLVRRDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1X1</accession>
    <name>U5W1X1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363612.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43022">
        <property type="protein sequence ID" value="AGZ43022"/>
        <property type="gene ID" value="AFR_23770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4815"/>
    <dbReference type="eggNOG" id="ENOG5032NYW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107608_0_0_11"/>
    <dbReference type="OrthoDB" id="1637933at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="18592" checksum="C7E60C724BA2A662" modified="2014-01-22" version="1">MTDEDLTLPAALAEAHDIGFFEDHDGYDLQPEETFLWSGEVTEWWRQWTGNASAEPPPLRVFGADGTGGLAAFWVREPAAALETQPLVFVGSEGEMHVIAKDLGDYLWLLAAGVGPLEPIEGIRSDPEPVPALVAVARRHSGLETRPVEAVLAAARAEHPALVEELTRCGGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1X4</accession>
    <name>U5W1X4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362299.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18240"/>
    <dbReference type="EnsemblBacteria" id="AGZ41926">
        <property type="protein sequence ID" value="AGZ41926"/>
        <property type="gene ID" value="AFR_18240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3702"/>
    <dbReference type="HOGENOM" id="CLU_1238036_0_0_11"/>
    <dbReference type="OrthoDB" id="1592099at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665988" evidence="2">
        <location>
            <begin position="23"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="23256" checksum="FEBB649C5DA9DD98" modified="2014-01-22" version="1">MSSAVVDVVIAVVLTLALTALAASAVVEWIGNLTHKRAKYLLRGLRNMLDAAGDAGRTAKSGPLAGAEAENAIYRKALAPGDGTTGDLTARLFDHPMLRAMMQPRRSAAGERTRVPPYVPAEMFSRALVDTLTPDDGDPLTLDRVRAAVAALDESMPARKALLGLAVRPAPPGKPPTGPRVSAIWSPRRTAPGARSGGPTTARGVSSPVWACRVRSPVPVTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Z5</accession>
    <name>U5W1Z5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alcohol dehydrogenase GroES domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_24315</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363719.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24315"/>
    <dbReference type="EnsemblBacteria" id="AGZ43129">
        <property type="protein sequence ID" value="AGZ43129"/>
        <property type="gene ID" value="AFR_24315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4925"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_3_11"/>
    <dbReference type="OrthoDB" id="787596at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027399">
        <property type="entry name" value="ADH_N_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13823">
        <property type="entry name" value="ADH_N_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ADH_N_assoc" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="ADH_N" evidence="4">
        <location>
            <begin position="26"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="ADH_zinc_N" evidence="3">
        <location>
            <begin position="196"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="390" mass="42214" checksum="271AD88F11323A15" modified="2014-01-22" version="1">MKALQWQGVNKLAVGDVPDPEIRNAGDVIVKVKRSVTCGSDLHLLGGYIPFMERGDVLGHEFIGEVVETGSEVRNHRVGDRVVVSSFIACGKCYFCEQRLFSLCDNGNTNPAITETLWGQAPGGCFGYSHAMGGNPGSHAEYIRVPFADVGAFTVPDAVTDERALFASDSASTGWMGADLGGVQRGDVVAVWGAGAVGQMAARAAVLLGADRVVVIDRYENRLHQVEKYAGAETLNYENTDVEAELRERSGGRGPDVCIEAVGMEAHTPGPQFLYDQVKQQLRLQTDRPAAVRDAIHACRKGGSVFVLGVYAGFVDKFPLGALMNKGLTVRGAQMHGQRYIPMLLDRMAKGELITEHLATHTMPLAEAPKGYRMFKDKEDDCVRAVFLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2E9</accession>
    <name>U5W2E9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">O-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_25745</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364091.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43413">
        <property type="protein sequence ID" value="AGZ43413"/>
        <property type="gene ID" value="AFR_25745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5219"/>
    <dbReference type="eggNOG" id="COG4122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067676_7_1_11"/>
    <dbReference type="OrthoDB" id="1948290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="22657" checksum="8536B662D03F843E" modified="2014-01-22" version="1">MPNTLTRSPARPVLDRLFAAAEHEEGQPSPWPAGRSFTDATAQERADLLDSVLMPVSAPGGTLLYTLVRACRPQTVVEFGTSYGISTIHLAAAVADNGTGHVISTELSATKVAAARENLAEAGLAGSATVLAGDALETLAGVPGPIGFVLLDGWKELCLPVLRLLEPRLTPGALVVADDTTFESMAPYLAYVRDPAHGYTTVDFPVADGMEISCRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2P8</accession>
    <name>U5W2P8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_25700</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25700"/>
    <dbReference type="EnsemblBacteria" id="AGZ43404">
        <property type="protein sequence ID" value="AGZ43404"/>
        <property type="gene ID" value="AFR_25700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5209"/>
    <dbReference type="eggNOG" id="COG4733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_386692_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PTHR11022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="SSF55846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGRP" evidence="3">
        <location>
            <begin position="222"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="714" mass="73462" checksum="C32B92AB3035FF22" modified="2014-01-22" version="1">MVAGSAVVVAGAAAVALNWPAGSTPGARGTEAVADAAAPVAAAQPVSATEPPRIDDGDGKPVSPRLHTVAIAPARKVDVPKRDTRQFSLLGMTWSDADLEVGGTVEVRTRSVRTGRWTGWQTLETEGTQGPDSGAEAKRDLRGGTEPLWVGPSDGVAARVVDAKGTKPLPAGLRLDLVDPGKPAGSSGGQGGGLALEPVPSAPAAAPEEAAADPVVEKVALPAYTSRAGWKAGPAKAAPAMGEAVKAMFVHHTADGSTTYDCKDSAAWVRAIQTYQVDSQGWDDIGYNFLVDRCGALFEGRGGGIDKPVVGAHTYGFNTDTASIAVLGTYSTSGVSDAARKVISQVSAARLTAYGFDPSTTAELTERATDGKFPYLSVQTFPRISGHRDGVATECPGNGLYAQLPAIRVEASARILALKASAPTGGVSSGGKYYVKGKATLAWTTATPSGSIAKFELLVDGKLITTLPATARSGSVTVPSGTHTVQVRGTHVSGAAETTAAATVISDVTVPVLSTPWLGLRTGTVSTTSVPLTVNFKATDNTRVTWIGSTSPTKVTLSPTATTWSTSARPGAAVTFGVSAKDLVGNVSTKSVARATALLPETSAKRTGKWTTKSVSSHLNGKALYSSKKDTKLTFTFTGRSAALVVGRFKTSGKATVYLDGKKVSTIDTKASKTTYRQAIWTRSPAYGKHTVTIVVLATKGRPGVTVDGLAYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2T2</accession>
    <name>U5W2T2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_19745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362593.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19745"/>
    <dbReference type="EnsemblBacteria" id="AGZ42221">
        <property type="protein sequence ID" value="AGZ42221"/>
        <property type="gene ID" value="AFR_19745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4006"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_9_0_11"/>
    <dbReference type="OrthoDB" id="972769at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="34117" checksum="00D06BFD85C7E945" modified="2014-01-22" version="1">MTGEARAFWLAAPGAGELRPVTLSDPGPGEVLVRTLHSGVSRGTETLVFRGGVPDSQHEAMRAPFQEGDFPAPVKYGYLNVGVVEQGPPELLGRTVFSLYPHQTRFVVPAESVTVVPENVPAARAVLAGTVETAVNALWDAAPLVGDRIAVVGAGMVGASVAAVLARFPGAKVQLVDADAAKATVAAALGVGFALPADATGDCDLVVHASATSAGLTRSLELLATEATVIELSWYGDREVSVPLGEFFHSRRLVVKSSQVGGIAPARRGRRSYADRLAIALDLLADPVFDALITGESDFEQLPEVLPRLTDGTLPALCHLINYPAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Z4</accession>
    <name>U5W2Z4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lipase 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362668.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20120"/>
    <dbReference type="EnsemblBacteria" id="AGZ42296">
        <property type="protein sequence ID" value="AGZ42296"/>
        <property type="gene ID" value="AFR_20120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4081"/>
    <dbReference type="eggNOG" id="COG1506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029538_3_0_11"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triglyceride lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005152">
        <property type="entry name" value="Lipase_secreted"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34853">
        <property type="entry name" value="PTHR34853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03583">
        <property type="entry name" value="LIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029171">
        <property type="entry name" value="Esterase_LipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665498" evidence="2">
        <location>
            <begin position="33"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="368" mass="37924" checksum="4F6A2B3481D07E8A" modified="2014-01-22" version="1">MRAIPRWTAVFQATLLSIFGVVVGSGPAAATAAPGTVLSSAAATLPPELASLGTGKRIEYVSTNLSGGAVTVTGLVITPKKKTHRTVVWGHGTTGIADQCAPSDHPAVFWPEARAAVAELLKRGWTVAAPDYPGLGTPAPHPYLVGGTTARSMIDSVKAARSLDSSLSTQYAIDGHSQGGQGALFAGQLAPSYDGPLVLRGVSAIAPVSNVDLLAPAIPGTPGQGYLVMALYGLNAVEPGFDPLSVLAQPAKQRVPVLQSGCLYEVLEKYMSLTPHQLLNGGALPPAVVSKLAYYDNPAQSAPSAPMLIVQGTDDEAVPYDITAGALLDQLDDYSQPVTFVPLEGQNHDGAVVASTTLVADWLAGRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W353</accession>
    <name>U5W353_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative signaling protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_25915</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25915"/>
    <dbReference type="EnsemblBacteria" id="AGZ43447">
        <property type="protein sequence ID" value="AGZ43447"/>
        <property type="gene ID" value="AFR_25915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5254"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="341"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="481"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="737" mass="78397" checksum="612369726C11E0BE" modified="2014-01-22" version="1">MVGAWTATGLAVALVYPFLPKGTLVTAVVFDLFVLATLVAVLAGIRRNRPVNSLGWYVFATGIGFRLAGDITYEIHRQVLHEAPFPSPADGFYLSAYPLLVVGSLLLTGSRRGRDLAGLLDAAIVATGLSLVWWVSAIGPIAANASIPMVQRLLGAAYPACDMLLLVVVARMLTRSGRPSVGLSLLTAGTTALLISDVFFQLVIIHAPELEGLVACGWILANVGWGAAALHPSAGRTVPVRPAQPWLGRRRLALLAASTLLVPTLLFVQGAARGAHQLNWLAIGIGAVVLFLLVLARMSGFVNQVQRQAQQLEDLAMRDDLTGLPNRRLFEERLAAAVLTGSPQVAMLDLNGFKDVNDRFGHAVGDRLLAVVAERLAGRLRGDDLVARMGGDEFAVLVATATPAEMAAIVDRLTASLRKPVEVAGHELLVGASVGVADSTGTDDPYEVLRRADVAMYAAKEHGGTRHRRYAPEMDERAGEEARLGAELRTALDTGQFRLVYQPIVSLPDGRIVSVEALIRWDHPDRGFVSPAEFIPVAEQNGLIVELGAWILRTACAQAMVWRNSLGDAAPERVSVNVSARQLAEPGFTELVASVLAWTGLGAQNLIVEVTETAVFGGGQAVQAVKDLHELGVRIALDDFGTGHSSLGLLQTVPVDVLKVDKSFVDNITMAGRHAVIATALIQVSNGLGLQAVAEGVETAEQATELHRLGYRLAQGYHFGKPVPEPDFRGAAVTIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3B6</accession>
    <name>U5W3B6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative HTH-type transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21090"/>
    <dbReference type="EnsemblBacteria" id="AGZ42490">
        <property type="protein sequence ID" value="AGZ42490"/>
        <property type="gene ID" value="AFR_21090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4275"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053650_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="2">
        <location>
            <begin position="46"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="27657" checksum="36BD5052F7968093" modified="2014-01-22" version="1">MNPSGAAAHDHQGPRAQGGRDNALMSIGEVLAQLRTEFPDTTISKLRFLEAEGLVDPQRTASGYRKYSWNDVARLRFVLTAQRDQYLPLRVIREQLEAQERGDDPLVHRRPSLVAVGSESEVPVDTADSRVSRADLVERTGIAEQMLSELEQIGLVVSRPPGWYDGDAVVIVEAVVGLVRHGLELRHLRAFRTGADREVGLFAQLLTPLMRQHDPAARARAAETARELQALSQRLHAALVRAGLRDTLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3B8</accession>
    <name>U5W3B8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxoglutarate dehydrogenase inhibitor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364286.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26250"/>
    <dbReference type="EnsemblBacteria" id="AGZ43512">
        <property type="protein sequence ID" value="AGZ43512"/>
        <property type="gene ID" value="AFR_26250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5320"/>
    <dbReference type="eggNOG" id="COG1716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031794_1_0_11"/>
    <dbReference type="OrthoDB" id="1632705at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SSF49879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="129"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="216" mass="22373" checksum="CED5DC83B65CBB32" modified="2014-01-22" version="1">MTTFACPKGHASTTDDFCDTCGAKIGGTAVFSPGVAPAAPPAFPSGEPCPNCGTPRAGAGRFCEDCGFDHSTGKVPVLTQPTTAAAPVTGQWTATIFADREYFEANKIEGVDFPEETGERTVSLPAPQVRIGRKSTSKGTDPEIDLADADPGVSHSHALLTLSVDGVWLVSDLGSTNGTWLNDEPRPLTAGQTRSVGDGDHVHVGAWTTITLHAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3M4</accession>
    <name>U5W3M4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27340"/>
    <dbReference type="EnsemblBacteria" id="AGZ43729">
        <property type="protein sequence ID" value="AGZ43729"/>
        <property type="gene ID" value="AFR_27340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5542"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074977_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="65"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="31358" checksum="7D2D4A87C797C0C7" modified="2014-01-22" version="1">MQMHAGQLDVPGETVRRLVDAQFPQWRGLPVRPVASEGTVNAIFRVGDGLTARFPLVPGDPGEIRADLEAEAAAAGLLAGRTRFPTPEPVAIGEPGAGYPLPWSVQTWISGTVATASDPGKSAKFASDLAEFIRGVRAIGTGGRTFSGRGRGGDLRAHAEGTETDLRNSEGLLDVPRLRGLWLDLISLPRRSPDVMNHGDLMPGNVLVSGGGLAGVLDVGGLGPADPALDLVGAWHLLESGPRQVLRDELGCDDLEWERGRAWALEQSLGLVAYYAETNPGMSRIGRRTLDRLLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3W4</accession>
    <name>U5W3W4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560161.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43824">
        <property type="protein sequence ID" value="AGZ43824"/>
        <property type="gene ID" value="AFR_27815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5637"/>
    <dbReference type="HOGENOM" id="CLU_2766531_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="69" mass="7435" checksum="FFD83483DDE43F23" modified="2014-01-22" version="1">MAIEPIGASRASDYLPRIELQRSQQKLTSDLAARAAEKLAVANEIDSTKAANEALAARQMSGGRIDMMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W425</accession>
    <name>U5W425_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TGc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560305.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28535"/>
    <dbReference type="EnsemblBacteria" id="AGZ43968">
        <property type="protein sequence ID" value="AGZ43968"/>
        <property type="gene ID" value="AFR_28535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5785"/>
    <dbReference type="eggNOG" id="COG1305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107900_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="26"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="20922" checksum="F52DF599F9637F24" modified="2014-01-22" version="1">MQLVAGEPTEYLVGDMVVEVDHPAVRALSGELRAAHPDDVDFTRAAFEWVRDRIRHSVDAQDPRITVTAGEVLSAGVGLCYAKSHLLVAVVRAAGVPAGLCYQQLNDGGEPFLHGLVAVYLEGRWHRQDPRGNRTGIDAQFRLGQEQLAYPITEGSAGRDLAEVHVQPSPNVITALRGAQDALELCRTGLPATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-02-10" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W454</accession>
    <name>U5W454_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alginate_lyase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22105"/>
    <dbReference type="EnsemblBacteria" id="AGZ42691">
        <property type="protein sequence ID" value="AGZ42691"/>
        <property type="gene ID" value="AFR_22105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4481"/>
    <dbReference type="eggNOG" id="COG3979">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038125_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008397">
        <property type="entry name" value="Alginate_lyase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008929">
        <property type="entry name" value="Chondroitin_lyas"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05426">
        <property type="entry name" value="Alginate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48230">
        <property type="entry name" value="SSF48230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666064" description="Alginate_lyase domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Alginate_lyase" evidence="2">
        <location>
            <begin position="104"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="396" mass="42517" checksum="ACBFE8DF4F4C160D" modified="2014-01-22" version="1">MLLATGTVVLAVLATVGVRFAASADTVVPARTTAAAAVFSHPGVLLSRPQLDFVKGRVAAKAQPWTAAYNQMMASRYASLTRTPKPRATVECGSYSNPNNGCTDEREDAIAAYTDALAWYITGNAAYATKAIALMDAWSATIKNHTNSNAPLQTAWAGSVWPRAAEIIKHTYTSWPNQGRFATMLRTVYLPVVIPGSGSNGNWELSMMEAAVGISVFLDDRASYDRAIARFKLRAASYIYVTADGSQPKYPPAGGIDTRAELISFWQGQSTFVDGLSQETCRDFVHTGYGLSAIAHVAETTRIQGGDLYPELQDRLRHSFGLHTKFENGTAVPSTICGGSVTKGLGPISEVAFNALHNRLGIAMANTETYTNAKRPQGSNNLFVAWETLTHAANPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4C9</accession>
    <name>U5W4C9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Strictosidine synthase, conserved region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560209.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28055"/>
    <dbReference type="EnsemblBacteria" id="AGZ43872">
        <property type="protein sequence ID" value="AGZ43872"/>
        <property type="gene ID" value="AFR_28055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5687"/>
    <dbReference type="eggNOG" id="COG3386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023267_0_1_11"/>
    <dbReference type="OrthoDB" id="966287at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016844">
        <property type="term" value="F:strictosidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Str_synth" evidence="1">
        <location>
            <begin position="143"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="341" mass="36362" checksum="C851B9EA551444B9" modified="2014-01-22" version="1">MTVPRPPAPWLIRPVKLPATTPPALIGPWAADDTRLDDVEKIEVPGGHGPEDVAVGADGQIFSGTEDGRIWRWPPDAHAGATPVLLADTGGRPLGIEADPRDGSLIVCDAYRGLLRVTADGTITDLTHRVGGSRILLCNNAAVARDGVVYFTDSSTRFPVSHWRRDLLEHRPNGRVLAYDPASGGTEVVAEGFHFPNGVALTPEEDALLLCETVSHRLVRLSLPGGKVTDLGDLPAYPDNMSAVGDGTYWIALASPRVAIAERLLPHPHLRRIAAVLPARLQPQPLPYTIAALVDGDGTVLRTLHGPAGRYVMATGVRQHGDTLWLGSLTESAIARTSLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4F7</accession>
    <name>U5W4F7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29060"/>
    <dbReference type="EnsemblBacteria" id="AGZ44073">
        <property type="protein sequence ID" value="AGZ44073"/>
        <property type="gene ID" value="AFR_29060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5888"/>
    <dbReference type="eggNOG" id="COG4225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030203_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666071" evidence="1">
        <location>
            <begin position="25"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="441" mass="47801" checksum="2558C8F6A9A27D97" modified="2014-01-22" version="1">MGLLLVVAGGSPASAASPTVNAPATTTLTTAGRTAESYTGLMNGESFQQDGIVSHRRWQYAAFWDDEGYINVSRRPTNGTWQNIRLTDYRTTTTDSHNVISIGLSHVDGSIHLSFDMHAQRFRYRKSVAGIATAPDTATWSPSIFGAVQNSLAGRDMAVMTYPQFVTMPDGNLQLTIRTGESGNGNQVLFEYRGGAWSFVGKIMDGTTLGNNAYLFGFQYDNQGLLHMTWTVRETFDASTNHDLLYAYSSDRGRTWRSGAGTLIGTADASPISTTVAAARFWTIGQNRGLINQESQTVDSAGNVHVLASHLPPSAPSDAVFTHARTSAVLVHYWREAATGQWRQRILGYQAGVSRGDIGVDSRDNLFVVSAASNTGVLQIATASRASLWSDWAVRYRSTPTFTSDPLLDHRLLKSNNIASVFAPHNGGSRIDVLNFTSAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4N5</accession>
    <name>U5W4N5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ECF subfamily RNA polymerase sigma-24 factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_23450</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23450"/>
    <dbReference type="EnsemblBacteria" id="AGZ42960">
        <property type="protein sequence ID" value="AGZ42960"/>
        <property type="gene ID" value="AFR_23450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4752"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_22_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="114"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="295" mass="32042" checksum="C68687D81F371540" modified="2014-01-22" version="1">MMTGSGVDLEEAAAVFTSLRPRLFGIAYRMLSSASEAEDLVQDVWLRWQTYDRSTVVNPGAFLATTTTRLAINALQSARVRRETYIGPWLPEPVDTSADPYLGAERGEALEFAALLLMEKLTPGERAAYVLREAFDYPYAQIADILSSTEPAARQLVSRARKHMADGRRTPVTAAEQKELLVTFIEAARSGNLAELERLFTPTVQNLSDGNGARQVARKIVVGPSRVAAFLHAISGHFWSGLEITWVSTNGGTSAVLRRDGSVYGVLTVSASADGIDQVLWMVNPEKIAAVVAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4X1</accession>
    <name>U5W4X1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363581.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23615"/>
    <dbReference type="EnsemblBacteria" id="AGZ42991">
        <property type="protein sequence ID" value="AGZ42991"/>
        <property type="gene ID" value="AFR_23615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4785"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_1_1_11"/>
    <dbReference type="OrthoDB" id="1942521at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665542" evidence="1">
        <location>
            <begin position="31"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="66"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="351" mass="37342" checksum="C04A9646F6A69236" modified="2014-01-22" version="1">MRLLSIRRTATVAAAGLILFLAACGPNNEADDTPAGGSSSAAAASAFPVSIENKFGTTTIPAEPKRIVTVGLVEQDALLALGVVPTATTEWFGEKPGAVWPWAEAALGSQPKPQVLTNTDGIQFEKVAALRPDLILAVYSGLTAADYATLSKMAPTVAQPKDSVDYGVSWQDLTRTVGTAVGRKDQADKLVTDTEARFETIKKDNPSFVGATAMMATTWEGYYVYGPQDPRGRLLTDLGFKLPDGLGEVTGKEFGANLSKERTDMLDTGLLIWLVDSYEPDKKKVQADPLYAKLKVKTEGRDIYLENEELVGAATSFISVLSLPFLLDKLVPQMAAAVDGNPATAVVRATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W533</accession>
    <name>U5W533_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Malate dehydrogenase</fullName>
            <ecNumber evidence="9">1.1.1.38</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_29280</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.38" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560454.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29280"/>
    <dbReference type="EnsemblBacteria" id="AGZ44117">
        <property type="protein sequence ID" value="AGZ44117"/>
        <property type="gene ID" value="AFR_29280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5933"/>
    <dbReference type="eggNOG" id="COG0281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011405_5_2_11"/>
    <dbReference type="OrthoDB" id="654531at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="malic" evidence="8">
        <location>
            <begin position="73"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Malic_M" evidence="7">
        <location>
            <begin position="265"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="554" mass="59910" checksum="82B65783A3732280" modified="2014-01-22" version="1">MSKQSGDRARTVLSDPLRSRGTAFDYEQRRRLGLTGRLPAVTETLDQQASRCYEQLSRIDTPIAQFIYLDLLHDRNQTLYFKVLADHLAELLPVVYDPTVGQAIKEWSRDYRVAQAAFLSIDHVEDIRVSLESFGLGADDVDLIVCTDAEEILGIGDWGVNGVEISVGKLAVYTAAAGINPGRVIAVSLDCGTNNASLLNDPTYLGNRHSRVSGQRYDRFIAEYLRIAAELFPNAILHFEDFGPENARRILDRYRGDYRIFNDDMQGTGAIVVASVLSGMKVTRQTFAEQRLVVFGAGTAGTGMADQLAAGMVRDGLSEEQAKDRVWLIDKHGLVVDDMVDLPGYQRAYARAADEVRGWKRTNGSIDLLTVIEEVKPTILIGTSTVHGAFTEEVVRALCTGVERPILLPLSNPTERIEVMPQDAIDWSGGKALVAVGIPTDPHDHDGVTYTIGQANNSLLYPGLGLGAIVSGATHITDGMLLAAAEAVASQVDPTPPGASLLPPVQNLRASSAIAAFAVAQAAARDGVATRAHDDLTQAVQDAMWQAAYPDAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5C9</accession>
    <name>U5W5C9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30470"/>
    <dbReference type="EnsemblBacteria" id="AGZ44354">
        <property type="protein sequence ID" value="AGZ44354"/>
        <property type="gene ID" value="AFR_30470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6169"/>
    <dbReference type="eggNOG" id="ENOG50331K6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110740_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019692">
        <property type="entry name" value="DUF2581"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10756">
        <property type="entry name" value="bPH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="15322" checksum="4D021934CA80AB02" modified="2014-01-22" version="1">MVFRPRRIRWVAGFSAAAVVVLFTVLSFGLHGSTGDTMGQFRRGDQSAMIGLGVLIGLGILAFTRPRVIADADGVQVRNVIGGYELPWGSIRAVRFDRNSPWASLEMLDDDTVPVHALQAADKEYAVEGVRALRALHSAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5N5</accession>
    <name>U5W5N5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_25170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3">Binds 2 manganese ions per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363890.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25170"/>
    <dbReference type="EnsemblBacteria" id="AGZ43300">
        <property type="protein sequence ID" value="AGZ43300"/>
        <property type="gene ID" value="AFR_25170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5100"/>
    <dbReference type="eggNOG" id="COG1690">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022279_3_0_11"/>
    <dbReference type="OrthoDB" id="1384936at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008452">
        <property type="term" value="F:RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017510">
        <property type="entry name" value="Release_fac_RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="PTHR11118"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="SSF103365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03073">
        <property type="entry name" value="release_rtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="active site" description="GMP-histidine intermediate" evidence="1">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="125"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="225"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="276"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="376" mass="39365" checksum="F69EE4C6B7F673B5" modified="2014-01-22" version="1">MSTVTVFASDGSWIESDAVDQCHQVAALEGVSHVAGMPDLHPGKGAPIGAAMMSTVLYPFLVGSDIGCGIAVFPIGLKRAVPEKLAGRFPDLDVEPDAGDPAWDVLDGDVPAGHLEGLGTVGRGNHFVELARIGTVLEPDHAARLGVAAGDVVLVVHSGSRGLGERILRAHTEVHGAGPAADPGAYLAAHDEAVRWGSLNRRLMAARVALALGAEPAAPIVDECHNLVEVRDGAYLHRKGAAPGDGRDVLVAGTRGTPSYLVAAHAGAEAGYSVAHGAGRKMSRSDALRRGRAKHTVEELRRTPVGSVVVCGDRQLLFEEAPTAYKRIEQVIADLAGHGLATPIATTVPLVTYKTAELGPRPRRDRQDHRRGRGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5V0</accession>
    <name>U5W5V0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_25555</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364016.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25555"/>
    <dbReference type="EnsemblBacteria" id="AGZ43375">
        <property type="protein sequence ID" value="AGZ43375"/>
        <property type="gene ID" value="AFR_25555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5178"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="34465" checksum="149C69337F669ADC" modified="2014-01-22" version="1">MTDVARLAGVSHQTVSRVLNDHPNVREQTRLRVRAAIAELGYRPNRAARALVTGRSQLIGIVAQNSTLYGPASLLAAFEQAAAEAGFAVSVGSVGRLDRESIAGAVERHLDQRVAGLVVIAPVKSADDALEDMPVGVPLVTIDGDPERSHTLVTVDQAAGARAATEHLLAAGHRTVWHISGPAEWYDAAWRIEGWRGALADAGAEVPPLVTGDWSASSGYDAGRMLARMPEVTAVFAANDHQALGLLRAFSEHGRRVPQDVSVVGFDDVPEAAFFIPPLTTVRPDFAAVAHAGLDLLLAQLTDEAAPPNRIVIAPSLVERASVGPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Y5</accession>
    <name>U5W6Y5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD_binding_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27420"/>
    <dbReference type="EnsemblBacteria" id="AGZ43745">
        <property type="protein sequence ID" value="AGZ43745"/>
        <property type="gene ID" value="AFR_27420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5558"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="10"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="388" mass="42419" checksum="66E0DDD8D2146F26" modified="2014-01-22" version="1">MIINENKGRILVSGASVAGLTAACWLDRFGFAVTVVERSAGLRPGGQALDIRGPALEVARRTGVLEDFRRRTTRLRGMSMVDDSGTEIHRSTERTLTGGRLDSEDLEVLRDDLVEILREACGPDVRFVFDDRITGLRQDEHGIDVDFRHAAPERFDVVIGADGAHSGTRRLVFGPEEPFLRYMGGYVAVMTIPNFLGLDHWQTFYLSENDSGAGLIGLDSSGTARAYLGFGSKEPVVYDHRDEDAQKQLLAERSAGLGWVVPQIIEHMKHDASGFHFDARIQVIMEHWTRGRVALVGDAGYAVSLTTGQGASMAMVGAYVLAGEIAAAAEDPATGIRRYEDKIRGYVLRNQAAARKLNDEDTLGPGDFTDFGTLVEDLPLHDYLPLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W726</accession>
    <name>U5W726_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Signal transduction protein with Nacht domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561281.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33430"/>
    <dbReference type="EnsemblBacteria" id="AGZ44944">
        <property type="protein sequence ID" value="AGZ44944"/>
        <property type="gene ID" value="AFR_33430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6766"/>
    <dbReference type="eggNOG" id="COG5635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_293330_0_0_11"/>
    <dbReference type="OrthoDB" id="809897at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1036" mass="114840" checksum="9A30E759A1ACDAD7" modified="2014-01-22" version="1">MSGSPYTLETAREILAGQRSPVVKALDKLLGYGILAAGPLGLATGHPWLLAAWQWVDQKNELMVHLDGLVATGRKKLGKATGRRRHELLAATHTALVTAAFFEALQAIAGPATGDLSDDDRRRLVGEPGTDQLFHDAVEQLLAVEIDLPWAGSGFDATVHSSIRPYYMTLAHRTLSWMSNFAAWTERFGTGTGPRPLHAQIVEHAVERYRAEYLALAAEVPEFHIWTVMGEHSAAQNALGRLEELVGGLVAAPRTRADAARDTLAALNRGVLGRPVVEADGLTAVRSPTVDRGYVEPHFRWAVMDRRSQPSREDWWSLQPEGTDLAGFLAAHFASPRAATLPLVVLGHPGAGKSLLTRVCAARLSTDAFTTVRIPLRDAPAGRIYRQVEDVLTETTHGKVEWRQLCEASKKRVRVLLIDGLDELMQATGATESNYLRDVADFQRAELEMDSPVAALVTSRTVVADTATIPQGCLVVKLMDFTDEQIDTWVQRWTEANAVAIREGHVRRIYPGALRTHDELARQPLLLLLLAMLASEQDLPEGATSAGLYRTLLDKFVARELDRPDVTGDDTNPEQKRRDELWKLGVVAFGMVNRGKQFLHEDDLAEDLRGLSSSRSARPPDPARRVIGRFFFVHTSEAEGGAGGRSYEFLHATFADYLIAHHAARLLREAATSWRTSGESQGWNDDLLFALLSHVVLGGSGSQSIDFFWDITDDPEELGPFLEHLVAAAHYRRRDNQFDSYDPSGRTVVQRIAVYTSNLVRLRVVLKQGPVAVAAFCPDDVEVETWWPAQVRLWEAVLSKTELETTIDYLSLDRSGVPTITWESTEGYYEDTLPWLALDRKNVIRSAAARGLLQPHYYEELIAPVGSDIADAAENLGFALRSHFGGPHIEESFVVEDENHPDLTQLRRLEISVMAKFAADFSYADVVRMIDRVDVEAEIPTLDRALLVVQHPRLLMNVPDMNAVRDEYGLINIMFALARLVPDDGREIFEELIETSEANRGIAETPEGRAVASLPPELGRRLIPELAKSILARQYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W769</accession>
    <name>U5W769_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative sugar kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_27625</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27625"/>
    <dbReference type="EnsemblBacteria" id="AGZ43786">
        <property type="protein sequence ID" value="AGZ43786"/>
        <property type="gene ID" value="AFR_27625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5599"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH marR-type" evidence="3">
        <location>
            <begin position="39"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="412" mass="42142" checksum="AF8387F8985BCC9D" modified="2014-01-22" version="1">MATPVRSGLSRAGRHPEVPLPAAPGASQEEIRRQNLGALLRYVHVHGPTSRAELTSRLGLNRSTIGALAADLVTAGLVTEEAPTTVRRAGRPSLVVSPCSERVYAIALSIEADRLRAACIGLGGRILDLREVPREPGSTALETIAPLAAVAREMAGGLAEDALCTGGGVAVADTTRDEDGRIRIGGADEELNAALDAEFGSGPAFVAGDMADIAALAEHIRGVAAGLDDLVYLHGDLGISAGIITGGRLVAGHRGHSGKVGHMVVNPDGLPCGCGSKGCWETEIGAAALLRHAGREPGEPGAVLDVLRAAARGERTSRAAVHQVAGWLGFGVANLVNVVNPDLVVFGGALREVYIAGAGVVRRRLDGIALPASREHVQLRAATLGRNAALIGAAELAFDRLLADPLNAGVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Y8</accession>
    <name>U5W7Y8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35385"/>
    <dbReference type="EnsemblBacteria" id="AGZ45333">
        <property type="protein sequence ID" value="AGZ45333"/>
        <property type="gene ID" value="AFR_35385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7160"/>
    <dbReference type="eggNOG" id="COG5635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010524_0_0_11"/>
    <dbReference type="OrthoDB" id="809897at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1054" mass="115663" checksum="E0EFFBFC4713610D" modified="2014-01-22" version="1">MRYADAVRLLGGASPAIKAADTLLGGALTIATAGGSDAALGLFDAKAEVIRLGHVVAGKVHDSVRGQARHDRSSRLQAAHGILVVSSFFEAVDEIIAATELDPPELTRDDQVLLVAGTPITGDWLRDLLGAPVPVPSAERSAVGLLDDLRDWYLREALHFGTYLQGLAAWERADQRSRRAVQRLLSEVLPAAALRRYEDSRRALAIDVPEFGLWVRRAEDRAVGRSLIELQEVLERTTSARDPDRRRRALAAAYRVELDKPVTGEASPELMIPALGEAYLDPLFRVKAAGPTARPAEHSWWDADIRSDFAAFLAEHLTTPQAAEAPLLLLGQPGAGKSALTRVLAARLPAADYLVVRVALREVPAEAEIQDQIEQAIRTTIGETVAWADLAATADGALPVIMLDGFDELLQATGVHQSDYLQRVARFQEREAVQGRPVAVIVTSRVAVADRARLPDGTLIARLEGFDDARVARWLRTWNSLNPTRAVQPEVVQRYSELCMQPLLLLMLVLYDATTGALREDTTFDTAQLYERLLHRFAEREVRKLHAGEPEPRMAGLVEAELTQLSVVALAMFNRLRQTVTQQDLDADLAALGLTTGRRAESEGFRSSLTAGEEVVGRFFFIQRSEALQDGRQLRTYEFLHATFGEYLVARLVVRTVRGNAARAAADDSPLGPATTDEGVLPGLLSYVPLIARGTVLPFISSMLATSKHEEIRSWLIDVLRVAMIRPEPARGRYQPVDKRVDHMMATYSLNLTLLVIACGKPLPASEIFVHAADPAAWFQGAAQQWLAAVPSGMWSAFAETVSIRKTWAGERRDLVLHRAAGLTPIPVDLLWTFPGIGHLADTSQPGVGLGVDSIDQMNSLLQLMGQEGGDVMLHAVEPLAAELPRSLTTFVVHEAGEVESIARSLTRVWLTSSLDGTTADLVEAYHRAVRAVEGRMWDPNEFDPSLVPAIRVVLRLLTADVTRIPAVTLDEWLLRLVTSPYFTQAHVPDALEFLVAASPIVDIRTMAERVVGKHADGTTKWSPADEIRVFRALRELQRCLPDSELDIRREPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Y9</accession>
    <name>U5W7Y9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34320"/>
    <dbReference type="EnsemblBacteria" id="AGZ45122">
        <property type="protein sequence ID" value="AGZ45122"/>
        <property type="gene ID" value="AFR_34320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6944"/>
    <dbReference type="eggNOG" id="ENOG5030RC6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822912_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666172" evidence="3">
        <location>
            <begin position="27"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="379" mass="39026" checksum="47B28CB20826CA31" modified="2014-01-22" version="1">MHSSRLAAVVTLVVAALIAVAPAASAAPPAPADLSIYFVGPEEIARGQTAMFLFQAHTETSPRPENVRAVLTLPAGLTWESAEPGAVGCPSYPGEPNCSRNSGTCTPDATRTVVTCTADEADDIMRGTYSYNATVRASADVAVGTELTVKAEFTSDGVEATPENNTSTVTSTVILGSDLGITAVPPAAPVVPGEPVKFSLIVHNYGPGTISFFRVAESYGGDWYMGGSMTNEGTECVPDPGALVCEVTMDLASGEEVKLDHVMPTRVVDYFWGTKQGGGAHVHNNPDETSFDTTNNRTSFKFEFAKKPAGTPATTPAGDGTGGTGGTAAGGLPITGAAALPLALGGALLLLLGTGAVLLTRRRKPRAEQSRNGRGDIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W866</accession>
    <name>U5W866_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560502.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29520"/>
    <dbReference type="EnsemblBacteria" id="AGZ44165">
        <property type="protein sequence ID" value="AGZ44165"/>
        <property type="gene ID" value="AFR_29520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5984"/>
    <dbReference type="eggNOG" id="COG2159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026290_2_0_11"/>
    <dbReference type="OrthoDB" id="1921782at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018891">
        <property type="entry name" value="Abortive_phage_infection"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10592">
        <property type="entry name" value="AIPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="576" mass="64864" checksum="5D4B5E5D9EB54F27" modified="2014-01-22" version="1">MDIVIDRLVKQFQEDHDLAALPGDEAFEAFTGYCVLSSYYESEFNPDDFRMGGGNDLGIDVAGVLVNGVLLRDEAEVRAAAEQARQLDVRIVVVQAKTSPKFEAKVIADLADNLSHIVAKDKLPYPASDDVENLRKCLTAIYENIAKFSGGRPQLHVHYATTGNQVADLLRRKAVSAEKMLMKSGLFDVVDVRCVTRDDLRELYKRATQAVPATFEMPKKMSLPRMPGVEESLLGLLSARELVEKVLTDPTGHIRTALFHENVRDFQGYNGVNAQIRDTIRDEIGHRRFAVLNNGITIVTRNLRVVGDEVHIRDFQVVNGCQTCHVLFDERSHLNNDVQVSVRVVHSADENVITGIIAATNRQTAVSEEDLSTREDFHKQLEDWFAAQDAPRRLFYERRSKQYAARQDVEKTRVINRSQLTRAYAAMFLGEPSAVGHYKDLTTRRKNDLFQAGHLPDAYYVAAATLYRIEWLLRTRRVRAGLRPVRYHLMAALRWQLLGDERLPASPKKASEQCRRLIELVWEPGKAEQAVLKLIPTLTEIIGKEEATGVPLGEMVRNRRFADAVRAAALGPRVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8P5</accession>
    <name>U5W8P5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="5">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsY</name>
        <name evidence="7" type="ORF">AFR_36435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561879.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36435"/>
    <dbReference type="EnsemblBacteria" id="AGZ45543">
        <property type="protein sequence ID" value="AGZ45543"/>
        <property type="gene ID" value="AFR_36435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7373"/>
    <dbReference type="eggNOG" id="COG0552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_0_11"/>
    <dbReference type="OrthoDB" id="656414at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF7">
        <property type="entry name" value="PTHR43134:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54" evidence="6">
        <location>
            <begin position="352"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="187"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="269"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="331"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="382" mass="40128" checksum="2DACC1F2D87733A5" modified="2014-01-22" version="1">MEYVVASLILLAVLVVGAIGLVVPRRRRRELPPPAGPSVPSVDELPPLIGEPEAPVVEEPVVEAPARPTIETPEPTAGRLVRLRARLSRSQSVLGRGLLSVLSRDHLDDDAWEEIEDSLISADVGVEATQALVERLKERVRVLGTRTVSEVRAQLAEELTAALDPGMDRSLRTTPHDGRPAVVLVVGVNGAGKTTTCGKVARVLIADGRTVLLGAADTFRAAAAEQLSTWGERVGAETVRGPEGGDPASVAFDAVKRGIEIGVDTVLIDTAGRLQNKVGLMDELGKVKRVVEKHGPVDETLLILDATTGQNGLEQARVFTDVVDVTGVVLTKLDGTAKGGIVIAVQNKLGIPVKLVGLGEGPDDLAPFEPAAFVEALLGTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8R3</accession>
    <name>U5W8R3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase C60 sortase A and B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_35730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35730"/>
    <dbReference type="EnsemblBacteria" id="AGZ45402">
        <property type="protein sequence ID" value="AGZ45402"/>
        <property type="gene ID" value="AFR_35730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7232"/>
    <dbReference type="eggNOG" id="COG3764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062592_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05829">
        <property type="entry name" value="Sortase_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042001">
        <property type="entry name" value="Sortase_F"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="24010" checksum="7A6105C0DEE284D0" modified="2014-01-22" version="1">MPDDVPEPETGTRRKALRAALPIVVSLLVAFVVATGYLMVREPRGSGDLGRWSGSASPAAVQPSEDMEGLPNPFHTAAPELGGPPTRLQVKAIGVDTSLETLHLGTDGELQPPKTNNQAGWYADGTAPGDIGPAVLAGHVDSKQGPAVFYRLREIEAGDKIEVTRGARTVTFTVTSTAWYPKKAFPTGKVYGPTPDRQLRLITCGGVFDQSLRSYKDNLVVYAVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W955</accession>
    <name>U5W955_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative preprotein translocase YajC subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_31055</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YajC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560806.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31055"/>
    <dbReference type="EnsemblBacteria" id="AGZ44471">
        <property type="protein sequence ID" value="AGZ44471"/>
        <property type="gene ID" value="AFR_31055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6286"/>
    <dbReference type="eggNOG" id="COG1862">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116157_1_1_11"/>
    <dbReference type="OrthoDB" id="2082059at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003849">
        <property type="entry name" value="Preprotein_translocase_YajC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33909">
        <property type="entry name" value="PTHR33909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02699">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01853">
        <property type="entry name" value="YAJCTRNLCASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01323">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00739">
        <property type="entry name" value="yajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="112" mass="12184" checksum="D3173C3C4A90CD6C" modified="2014-01-22" version="1">MPLLLIVLLFGVMYFMMIRPQQKRRREAQQMQSSLGAGDQIVTIGGLHATVVSVDDDVVTVEISPGVNARYARPAIAKIVKQEATVVEEPVAAPVADDPIESPVVETRKSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9L0</accession>
    <name>U5W9L0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562233.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38220"/>
    <dbReference type="EnsemblBacteria" id="AGZ45898">
        <property type="protein sequence ID" value="AGZ45898"/>
        <property type="gene ID" value="AFR_38220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7732"/>
    <dbReference type="eggNOG" id="ENOG5033D21">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148727_5_3_11"/>
    <dbReference type="OrthoDB" id="2073054at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028037">
        <property type="entry name" value="Antitoxin_Rv0909/MT0933"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14013">
        <property type="entry name" value="MT0933_antitox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="59" mass="6403" checksum="0FE07A97F118D5CD" modified="2014-01-22" version="1">MGFMDKAKDMADKHDEQVDQGLEKAGDQIDARTGNKHSDQIDKGVDQAQARTGAGDTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9W9</accession>
    <name>U5W9W9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major Facilitator Superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32585"/>
    <dbReference type="EnsemblBacteria" id="AGZ44775">
        <property type="protein sequence ID" value="AGZ44775"/>
        <property type="gene ID" value="AFR_32585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6594"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_17_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="419" mass="43527" checksum="CB28F39674D58418" modified="2014-01-22" version="1">MAEVETPDGDDSEPRHLPTDRPLSSPPFRWFFIGRSVSLAGSFMSPVALSFGVLEITGSGAWLSAVLAAAVIPMVATMLIGGGIADRYRRDSVLRLTSLGAGITQAGVAVVLLTHQRPALLLPLAALNGVFQGLTTPTLRGILANLVSGRALQQASSQLASAGNTARILGPSAAGFLTASAGGGWAIAADAATFLAAAACFARISLPGRPPRAADDPSMLSALAEGWKYFRTRTWIWSVTLAFAIFNATTMGFWQVLGPVITNDTIGPGGWGLALSARGVGALLAGLILVKIPIMRRPMTPALTAMALAALPLILLGASSNLAWLMTATFIAGIVAEFFTFVWVTVTNTHIPERLLSRVGAHDEFWSFLSLPVGQISASALTEAVGSTTVAYAGGGIGAAAMLLTALVPSLRRIEINNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Y0</accession>
    <name>U5W9Y0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561058.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32340"/>
    <dbReference type="EnsemblBacteria" id="AGZ44726">
        <property type="protein sequence ID" value="AGZ44726"/>
        <property type="gene ID" value="AFR_32340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6545"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_787368_0_0_11"/>
    <dbReference type="OrthoDB" id="1712291at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="60"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="35769" checksum="BBE5B7DE87B81FEA" modified="2014-01-22" version="1">MISFAEATARIERAGSFGDLIDSGPGAAVVDAGHAYRMLVKVVHPDVAPAGLGVTATAAFAKLSALWAGRDGRVLTTRRATYRVGGVVASGDIAELHTIDGDAALLKLPRRPGDNDLMQAEATALKKLASDGDPKFRAYAPRLIETFTHEDAAKARRTAVIMERQRGFVSLAEVRAAYPDGLDPRDVAWMWRRLLTGLGWAHRAGLVHGAVLPEHVLIHPAEHGVVLVDWCYSVAPGATIPALVATHRDAYPPEVTGKQPATAATDIYMATGLMLGLIGRPPEALRRFARGCLYDAPRMRPQDAWSLLGEFDELLENLYGPRRFRPFAMPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA89</accession>
    <name>U5WA89_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetracycline repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32990"/>
    <dbReference type="EnsemblBacteria" id="AGZ44856">
        <property type="protein sequence ID" value="AGZ44856"/>
        <property type="gene ID" value="AFR_32990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6676"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TetR_C_1" evidence="1">
        <location>
            <begin position="44"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="19735" checksum="4282036ACBE940A9" modified="2014-01-22" version="1">MRRLAAELGAGVMSLYTYVRDKEELLDLMIEQVSDPGHELDDADLLGLTTWQRALMLRHPWIPEALPNRQLSGPNMLGFLERGLAALSGTGLPGPAKMEILALLTGFVASYVTNELAQARIGVTDSERVREHVDRLRTAATSGDYPELAKVLSQGVSGPPPSFEGIAERMIAGLVTGQPSPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAP7</accession>
    <name>U5WAP7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative uroporphyrin-III C-methyltransferase/uroporphyrinogen-III synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_40170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562620.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40170"/>
    <dbReference type="EnsemblBacteria" id="AGZ46288">
        <property type="protein sequence ID" value="AGZ46288"/>
        <property type="gene ID" value="AFR_40170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8130"/>
    <dbReference type="eggNOG" id="COG0007">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_6_0_11"/>
    <dbReference type="OrthoDB" id="1185397at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033014">
        <property type="term" value="P:tetrapyrrole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="SSF69618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="HEM4" evidence="6">
        <location>
            <begin position="262"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="528" mass="55086" checksum="7583917B379001F8" modified="2014-01-22" version="1">MTTRTARKSAGRIAFVGAGPGDPGLLTRRAHDTLAEADQVIYDRGVPESLLAAIRAEAKDDTQFSPAEGAPGDVAKVLLSAAKSGLAAVHLVAGDPFGHEAVVKEVQAVARTAVHFEVVPGLGQAEGVATYAGVPLPGVRTAADIDDVTALDFDALAAAAVKGSFAVAVDAGDLAAVRDGLLAAGVEPGTPVGVTGDGTGETQYTTTSSVDSFVAAALGFTGRVVLTVGSEVAQRDKLSWWENRPLYGWKVLVPRTKEQAGAMSARLRAYGAIPCEVPTIAVEPPRTPAQMERAVKGLVDGRYAWVVFTSVNAVRAVWEKFAEHGLDARHFGGVKIACIGEATADAVRAFGIQPELIPAGDQSSEGLLAEFSPHDEILDPVGRVLLPRADIATETLAAGLIERGWEVDDVTAYRTVRAAPPPADIRDAIKSGGFDAVLFTSSSTVRNLVGIAGKPHARTVVAVIGPKTAETATEFGLRVDVQPQHASVPDLVEALASYAVELREKLAAMPAKQRRGSKVQGPTALRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAV6</accession>
    <name>U5WAV6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleoside triphosphate pyrophosphatase</fullName>
            <ecNumber evidence="2">3.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Nucleotide pyrophosphatase</fullName>
            <shortName evidence="2">Nucleotide PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Nucleoside triphosphate pyrophosphatase. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside 5'-phosphate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23996"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Maf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.9" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39365"/>
    <dbReference type="EnsemblBacteria" id="AGZ46127">
        <property type="protein sequence ID" value="AGZ46127"/>
        <property type="gene ID" value="AFR_39365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7964"/>
    <dbReference type="eggNOG" id="COG0424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040416_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036218">
        <property type="term" value="F:dTTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="PTHR43213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="19721" checksum="E7C31F736C671DB2" modified="2014-01-22" version="1">MLHAAGIDVDVRVSGVDESIVKATDAGELCLELARLKARAVAATLPAEPGVLVLGCDSVLAFDGQILGKPHTAVEAVRRWTAMRGKQGVLHTGHHLTDLTTGKQAEEVGRTVVHFADLTDQEIEAYVASGEPLHVAGAFTIDGKGGAFVERIEGDAGNVIGLSLPLLRRLLMTFGLTVPQLWRREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB25</accession>
    <name>U5WB25_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561512.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34595"/>
    <dbReference type="EnsemblBacteria" id="AGZ45175">
        <property type="protein sequence ID" value="AGZ45175"/>
        <property type="gene ID" value="AFR_34595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6998"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119884_3_0_11"/>
    <dbReference type="OrthoDB" id="1984222at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="18332" checksum="E37149311E57088D" modified="2014-01-22" version="1">MGNATITAWCAAGESGDAAAAAVCLAPEVELISPLTAQFRFHGPAAVTDVLASAFEVISDIRFHTQVGEGDTWALFYRGRVKAEELEEAQLLRLNKDGLIREITFFGRPLPALTEVMAGIGPRLLRRQRQPILARIVGLATGPLTAMAKSGEKRILPRADPARKRDTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBI4</accession>
    <name>U5WBI4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">4-diphosphocytidyl-2C-methyl-D-erythritol synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">MEP cytidylyltransferase</fullName>
            <shortName evidence="7">MCT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ispD</name>
        <name evidence="8" type="ORF">AFR_41275</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>2-C-methyl-D-erythritol 4-phosphate + CTP + H(+) = 4-CDP-2-C-methyl-D-erythritol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="EC" id="2.7.7.60"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.60" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562841.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41275"/>
    <dbReference type="EnsemblBacteria" id="AGZ46509">
        <property type="protein sequence ID" value="AGZ46509"/>
        <property type="gene ID" value="AFR_41275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8353"/>
    <dbReference type="eggNOG" id="COG1211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061281_1_1_11"/>
    <dbReference type="OrthoDB" id="1836139at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00093"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050518">
        <property type="term" value="F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00108">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001228">
        <property type="entry name" value="IspD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018294">
        <property type="entry name" value="ISPD_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00453">
        <property type="entry name" value="ispD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01295">
        <property type="entry name" value="ISPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5 7 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="7">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="7">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="23100" checksum="D4C2E5A2436B27F5" modified="2014-01-22" version="1">MTAQLNARGDVAVLVPAAGAGLRLGPGGPKALRLLSGEPLLVHAVRRVAAAPSVRLIVVAAPAAEVEAVRALLAPVAPVTVVAGGAERQHSVAAALAIVPADVGIVLVHDAARALTPPELVESVAAAVRAGHPAVIPALPVVDTIKEVGPAEVVLGTVDRSLLRSVQTPQGFRHDVLAAAHAAGLEAHTDDAGMVEKAGVPVTCVPGSELALKITRPLDLVIAEALLHVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC00</accession>
    <name>U5WC00_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator of G-protein signaling 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41315"/>
    <dbReference type="EnsemblBacteria" id="AGZ46517">
        <property type="protein sequence ID" value="AGZ46517"/>
        <property type="gene ID" value="AFR_41315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8361"/>
    <dbReference type="eggNOG" id="COG2847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1114833_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666268" evidence="2">
        <location>
            <begin position="32"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="24852" checksum="4368CAD37BBD9AAB" modified="2014-01-22" version="1">MRSLGSRRAALVTGVAAVAAVALAGCSAGQVAETALKRPSNMGVNADSSDKSVYIRNLSVQYPGTAGYAAGDSAPLELGIFNQTKQPITVLVSSRPVTDESVKDEIVSARVIGLSGGAAATPSAPSSAIPEPSGSRPSGGQDDQNSEQIPSPDPSDGPSPAASPSAATPAVRPARIELEPLGAATFLPGDKETLQAVGLSGKLVPGQSVSLVFEFSNGAEPLVLQAPVAIPLSPASRGPGIPHENSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC31</accession>
    <name>U5WC31_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563099.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42570"/>
    <dbReference type="EnsemblBacteria" id="AGZ46768">
        <property type="protein sequence ID" value="AGZ46768"/>
        <property type="gene ID" value="AFR_42570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8615"/>
    <dbReference type="eggNOG" id="COG2013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057502_2_1_11"/>
    <dbReference type="OrthoDB" id="1063432at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="SSF51219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25729" checksum="5D08D1353CE294D3" modified="2014-01-22" version="1">MRSELFSADNLEKESNQPGLRLQNSKLLKAELNGEFMARVGSMVAYQGQVQFEALGSGGLGKFLKQKLTGEGVPLMKVTGRGDVFLAENAADIHMIDLEPGDALSINGANVLAFDSTLNYDIKMVQGVGMMSNAGLFNCVFSGYGRIAVTTKGTPVVLSVDQPTYVDPQAAICWSANLQTGYHRAEQMGLGTLLGRSTGERFTMSFAGQGFVVVQPSEEPPGGLAGAAQGQQQSGGILGNLMGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC67</accession>
    <name>U5WC67_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_41490</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41490"/>
    <dbReference type="EnsemblBacteria" id="AGZ46552">
        <property type="protein sequence ID" value="AGZ46552"/>
        <property type="gene ID" value="AFR_41490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8395"/>
    <dbReference type="eggNOG" id="COG0053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_424420_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666274" evidence="3">
        <location>
            <begin position="19"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="391"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="646" mass="69411" checksum="76CE4674F94F14D9" modified="2014-01-22" version="1">MVLTIMVALVASIAWAVAAPSAATAGPLQAPGGACSTEEWKNDLRGCVDRLTDVAGDRAECLKPPTPSAPDSGLAGWFAERPESSTKNGPQGIYSQYGYAGYSYTTYDLEGGCASTLIDPEYKFETTVANGEFMIATAVIGASNALRERAWDPQALWGWADPLVEQATKAVYEKVFSVFGVITLAVVGLYLIWRSQQADMGAATTTAGWAILVMVAVTAIAAWPVKSANIADQSLITTLGVVHDAVGPRPQDGDATAGCKLGKADACQDNRPPAVRASDTATDTMLYRNWLRGLLGSADSVTAQKYGRALYDARSFTWDEAQKMRDNSDTRKPTITAKNEQWEKVAQQIQNEDPEAYEYLRGVNGMDRVGAGFIAVLASIMFAMFDLTASLLVLLGFLIFRWAVIAAPILGTVGLLRPASSGIRRLANAVVAAIFNIALFGTGAAIYLFAVDLIMNTASLAGWLQVVLVWLCGVVGWLLLRPYRRITQLGGKDSTSAITSAGSWHRRFFRDMREAAKLDVADAGGTNEPRIGKSRGVYVEQTNLRPEARLEDPAHAALSRNNPAAPSREVTAPGGRPDGNESTRDSAPNPDHRPVPVTASAAKQRRSSTWTEPDVTERPASYAIYRPETGSTTREPASPRVRTEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCR4</accession>
    <name>U5WCR4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylglucosamine 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Enoylpyruvate transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UDP-N-acetylglucosamine enolpyruvyl transferase</fullName>
            <shortName evidence="7">EPT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murA</name>
        <name evidence="9" type="ORF">AFR_37310</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562051.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37310"/>
    <dbReference type="EnsemblBacteria" id="AGZ45716">
        <property type="protein sequence ID" value="AGZ45716"/>
        <property type="gene ID" value="AFR_37310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7548"/>
    <dbReference type="eggNOG" id="COG0766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027387_0_0_11"/>
    <dbReference type="OrthoDB" id="537477at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate binding" evidence="7">
        <location>
            <begin position="25"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="428" mass="44667" checksum="D73E5F6BC864E53F" modified="2014-01-22" version="1">MARVDVIRVKGGARLSGDVSVGGAKNSALKLMAVALLAEGRSVVSNVPRITDVAIMAEVLRRLGCEVSLENGEATIDVPAEPGSETDYDLVRRLRASICVLGPLLARRGYVRVAHPGGDAIGSRGLDMHVAGLARMGAEISGEHGFVIASAPGGLHGGKIWLDFPSVGATENLLMAAVLAKGVTEIDNAAREPEIVDICDMLTAMGAQITGAGTSTLTIEGVDGLKPVQHTTVGDRIVGGTWAFGAAMTRGDVTIHGVRPEYLAIALDKLVSAGAVVEPGDNQFRVRMDDRPKAVDIVTLPYPGFATDLLPMAIGLAAVSSGSSLITENIFDGRFMFVNEMVRLGADIRTDGHHAVVNGRERLSGAPVRATDIRAGAGLVIAGLCADGVTEVGEVHHIDRGYPDFVSDLANLGVDVERGVVTEPTFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCZ1</accession>
    <name>U5WCZ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37760"/>
    <dbReference type="EnsemblBacteria" id="AGZ45806">
        <property type="protein sequence ID" value="AGZ45806"/>
        <property type="gene ID" value="AFR_37760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7636"/>
    <dbReference type="eggNOG" id="ENOG5032RMD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122776_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="14860" checksum="A847490BDA026D31" modified="2014-01-22" version="1">MADPSPASTRPRDAAIGTGAGRVLLLVYGIFALSAGARAGVQIATKFSEAPLAFLLSAFAALVYLAATAGLAIGGARGRMIALTSCSIELIGVLVVGTLSIFDREAFPDATVWSQFGSGYGYVPLVLPLIGLWWLWHNRPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD31</accession>
    <name>U5WD31_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38380"/>
    <dbReference type="EnsemblBacteria" id="AGZ45930">
        <property type="protein sequence ID" value="AGZ45930"/>
        <property type="gene ID" value="AFR_38380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7764"/>
    <dbReference type="eggNOG" id="ENOG5031XF0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109580_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="25857" checksum="6245C51A697B7C71" modified="2014-01-22" version="1">MDQGVSAAGLPDAVDGGQHRRRAPIVTGQRVGLIALLVAVAVATVAVPPLITPSGDKPAAAPPVPFSQPPSPSSPSKTPPSPSPAPRKALAACAPVAEGSSVEVTARPSCVVYGPAVGNGWRATGDGLKVIPGEFLPGRKERGLRVERTRPALDSTAMALVAATPVGLRSGDTLKLRVWGGREFGTVLRVGVTPGSGTVKLTAPAERWTSYSIKLADLTSRDRLSRIDLVVAADEVPNVTNFFLDDITLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDN5</accession>
    <name>U5WDN5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46125">
        <property type="protein sequence ID" value="AGZ46125"/>
        <property type="gene ID" value="AFR_39355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39355"/>
    <dbReference type="HOGENOM" id="CLU_2550677_0_0_11"/>
    <dbReference type="OrthoDB" id="2219794at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665802" evidence="1">
        <location>
            <begin position="23"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="82" mass="9371" checksum="39076F313B091DCD" modified="2014-01-22" version="1">MMVLLRSYMMTRAWLFSCSCWADVTGGLSWAGVWIDVVDLAGASFLACQARFFWSFASYWSRIGFSLWRARLSVSVTVEKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEC4</accession>
    <name>U5WEC4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40160"/>
    <dbReference type="EnsemblBacteria" id="AGZ46286">
        <property type="protein sequence ID" value="AGZ46286"/>
        <property type="gene ID" value="AFR_40160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8128"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043786_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="321" mass="35453" checksum="98CEB3D2B75ED8EA" modified="2014-01-22" version="1">METLNAVLAADLPDDPPWQDVQVVDYLAETMPGERRISWVAEDDRLAEGESAIFGHVSILLLGDIGVLEVLVHPDLRRRGLGRELVAVAARRAYLEGFSSIGVEAIGDTPAIAFYQSLGFEQEYVETRSVLTLSSVDWSALGAMAGGISSGYRVEYHPGGPPAELLEAYAQAKLEGQDGDDSELDLRPSSSDPQRLRDSLDTLHRRGLKPYIVLATHEASGAVAGLTEVVVPAQHPERADQYDTIVVREHRGYGIDRAIKARMLFELRNAEPGLREVQTWNAQHNESMLKVNAELGFQSDRDWFEYGADVAQLVQRLEPHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFB9</accession>
    <name>U5WFB9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1524 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41760"/>
    <dbReference type="EnsemblBacteria" id="AGZ46606">
        <property type="protein sequence ID" value="AGZ46606"/>
        <property type="gene ID" value="AFR_41760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8451"/>
    <dbReference type="eggNOG" id="COG2356">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043034_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011089">
        <property type="entry name" value="DUF1524"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07510">
        <property type="entry name" value="DUF1524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666534" description="DUF1524 domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1524" evidence="2">
        <location>
            <begin position="104"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="23836" checksum="DCE94229F6F0B67C" modified="2014-01-22" version="1">MPPLSAPKWTLRAAAAFLVLITATACEVVPPEDSGSAPGSGTSTADARDAQTELGKLKIAKAGSMRGYSREKFPHWNTTGSNCDVRDTVLKRDGTKVKVSGCNVVAGTWTSIYDGDKITDPGKVDIDHMVPLANAWRSGANAWTTEKRQDFANDLDDPQLVAVSASSNRSKGDQDPSTWKPERSAVWCEYAQDWITVKSKWKLTVTTAEKAALTDMLEKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNI6</accession>
    <name>U5VNI6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357471.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01195"/>
    <dbReference type="EnsemblBacteria" id="AGZ38528">
        <property type="protein sequence ID" value="AGZ38528"/>
        <property type="gene ID" value="AFR_01195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.238"/>
    <dbReference type="eggNOG" id="ENOG5031VSU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2721168_0_0_11"/>
    <dbReference type="OrthoDB" id="1884284at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="77" mass="9094" checksum="AB8F655E091DC6C4" modified="2014-01-22" version="1">MEQRKHWWNGRWGRVARKDVYLRTSGDQWYVEQRAGGSDGVSHFFEYDSEEAALDMVRALLVGPDEWRELSVRPPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNU1</accession>
    <name>U5VNU1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Polyprenyl synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_00890</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00890"/>
    <dbReference type="EnsemblBacteria" id="AGZ38467">
        <property type="protein sequence ID" value="AGZ38467"/>
        <property type="gene ID" value="AFR_00890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.177"/>
    <dbReference type="eggNOG" id="COG0142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="387" mass="40939" checksum="705AC4089BD28A06" modified="2014-01-22" version="1">MTVANDILEGNPRFGIPRQTVSPGDLVRAVEGTLAEFLSGQTTALDDVDPALGRFARTTRDLVLAGGKRLRPTFAYWGWRGVVGPHEPVEPVLPALSALELMHTFALVHDDVMDDSATRRGGPTAHRIFAAQHSNAGRLGDPDHFGTTSAILVGDLCLVWADQLLARSALSTTTHFAVRSCYDRMRIEAVAGQYLDVLGETEPSAWSVERALLVARHKTASYTVQRPLQFGLALAGTVDIDVAAVDEAYNTYGIAVGEAFQLCDDLLGVFGDPVVTGKPAGDDLRTGKPTALLMLARQLATPAQRAELDPDPAGAVNPADGSGGSISSADLARRTEVIVETGAVAQVEALIRRRVAEGIEALAGAPIHDDARAALTELAVTSTDRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP34</accession>
    <name>U5VP34_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357666.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02170"/>
    <dbReference type="EnsemblBacteria" id="AGZ38723">
        <property type="protein sequence ID" value="AGZ38723"/>
        <property type="gene ID" value="AFR_02170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.439"/>
    <dbReference type="eggNOG" id="ENOG502ZCFK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021151_2_0_11"/>
    <dbReference type="OrthoDB" id="1770401at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021421">
        <property type="entry name" value="DUF3071"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11268">
        <property type="entry name" value="DUF3071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="358" mass="38118" checksum="2D6BD05F47B731D3" modified="2014-01-22" version="1">MRPVRFVALSEDGQALVLADEVGRLLALPIDDRISGALQSDGTPHPIGTAVAVANAEVMASLSPRDIQARIRSGESAEDVARVAGVPVDRVLRYAGPVLQERAMLAQHARRTRLKTSDSGAPLAEVVDSRLSQHGIDTEKISWDAFRREDGTWRIVATWPSGKATAQAMWDLDKGRQVVAPHDDMAQYLCAERPTQILGQEPVPERTPHERGGHGLPGPSRINEPSRGGRGLPSTDSPRPTRDPIRAGRDALLASLDRPLNQGSGRGLEAAPQESRRPVAGGAAALLGGGPGSAFDDDADLPKEVPAVPSLAVLRPRRTVGQPQGESSESEETAKPRKRLPSWDDVLFGGGPAARESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPE2</accession>
    <name>U5VPE2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357620.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01940"/>
    <dbReference type="EnsemblBacteria" id="AGZ38677">
        <property type="protein sequence ID" value="AGZ38677"/>
        <property type="gene ID" value="AFR_01940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.393"/>
    <dbReference type="eggNOG" id="ENOG5031PIK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179042_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022536">
        <property type="entry name" value="EspC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10824">
        <property type="entry name" value="T7SS_ESX_EspC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="98" mass="10458" checksum="A04F0A12E34DEEBF" modified="2014-01-22" version="1">MEKLAERLDSAADALTTVDRSLSAHAASASAFGADDEGVPGRLGRQLHERWLAVLAARSQEAAAAAARLTELAADVRLAAKNYTETDDEVARRIRRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPU1</accession>
    <name>U5VPU1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02690"/>
    <dbReference type="EnsemblBacteria" id="AGZ38827">
        <property type="protein sequence ID" value="AGZ38827"/>
        <property type="gene ID" value="AFR_02690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.541"/>
    <dbReference type="eggNOG" id="COG1086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013560_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05237">
        <property type="entry name" value="UDP_invert_4-6DH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003869">
        <property type="entry name" value="Polysac_CapD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020025">
        <property type="entry name" value="PseB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02719">
        <property type="entry name" value="Polysacc_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03589">
        <property type="entry name" value="PseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polysacc_synt_2" evidence="2">
        <location>
            <begin position="6"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="35786" checksum="44C9DAE4A0FF05EA" modified="2014-01-22" version="1">MSGSSILVTGATGSFGKAFLGHALANLDPQRIVVFSRDELKQYEVRQMFGDDPRLRFFIGDIRDRDRLARAMHGVDHVVHAAALKQVDTAEYNPSEYVATNINGSQNVIDAAINAGVQRVVALSTDKASSPINLYGATKLVADKLFIAGNHYAATHPTRFAVVRYGNVMGSRGSVVPLFRRLNAEGKSLPITDKRMTRFWISLDAAVKFVVDSFDVMQGGELFVPRIPSMRILDLVEAVAPDAPTHEMGMRPGEKLHEEMIAADDSRRTLRFPDRYVVQPVVASWGYQTPEGGERVADGFNYRSDNNDLWLEVDEMSALLEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPW2</accession>
    <name>U5VPW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357787.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02775"/>
    <dbReference type="EnsemblBacteria" id="AGZ38844">
        <property type="protein sequence ID" value="AGZ38844"/>
        <property type="gene ID" value="AFR_02775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.557"/>
    <dbReference type="eggNOG" id="ENOG5030MUQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840806_0_0_11"/>
    <dbReference type="OrthoDB" id="1987467at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="14378" checksum="85650896FA343FAD" modified="2014-01-22" version="1">MTSTLSSAEVSRWRAAVGDSRIGEPVAGADLTRLPPDLRAFYQVVGEVSLPDVENGYWIHRPPGPGVDNGQPYVLSDGRPIVVFGSDGGGALFALCGSAVLRLAGGAEVGGVYDEDGATVVAADLREFLALLLRGAGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ69</accession>
    <name>U5VQ69_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357902.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03350"/>
    <dbReference type="EnsemblBacteria" id="AGZ38959">
        <property type="protein sequence ID" value="AGZ38959"/>
        <property type="gene ID" value="AFR_03350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.674"/>
    <dbReference type="eggNOG" id="COG3795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130902_2_2_11"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="11856" checksum="A4E21535CE8BAF86" modified="2014-01-22" version="1">MKYMMFVCTDTEPETDDSLVPDVDVWVTENDKAGRRIEGSELVSPAAATTVRVRGGELLLSDGPFAETKEVIVGYDLLECADLDEAIEVARAHPMAWRGRIELRPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQA4</accession>
    <name>U5VQA4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NLP/P60 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_04070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04070"/>
    <dbReference type="MEROPS" id="C40.007"/>
    <dbReference type="EnsemblBacteria" id="AGZ39103">
        <property type="protein sequence ID" value="AGZ39103"/>
        <property type="gene ID" value="AFR_04070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.822"/>
    <dbReference type="eggNOG" id="COG0791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034085_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665138" evidence="3">
        <location>
            <begin position="27"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="NlpC/P60" evidence="4">
        <location>
            <begin position="208"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="34"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="35031" checksum="1FB122F80FF7566E" modified="2014-01-22" version="1">MPLTPRKIIVALVPVLLLLGLSPAPAAGADRPTAAELDKRITAASRQLEVLVEQYNNSREDLNATRMQTKHLGGRLGPMTRELNQQQEVMADLASRTYQRTRNGPTVALFAADQPQLFVDKLLVLHQLATSEQRAVAELRAARSKVVQTRKMLNALAAQQRRQQFQLRARKATVEGEIGALKQMRAIAYGGGSRYGVSSDVPMPDFIPGRAGRVVAFAFAQLGKPYRWGAAGPNSYDCSGLTLAAWNTAGVGLPHNAARQYGSMAHISRSDLRPGDLVFFYGPISHVGVYIGGGNMIHAPEYGENVRVSSIDTQPIHGFGRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRB9</accession>
    <name>U5VRB9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetylglutamate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358888.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05340"/>
    <dbReference type="EnsemblBacteria" id="AGZ39357">
        <property type="protein sequence ID" value="AGZ39357"/>
        <property type="gene ID" value="AFR_05340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1081"/>
    <dbReference type="eggNOG" id="COG1246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119519_0_0_11"/>
    <dbReference type="OrthoDB" id="1823496at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="18999" checksum="7DA869CD09994DC9" modified="2014-01-22" version="1">MQVEIRRARTSDVRSIRALVDTYTSDRRLLSKATVTLYEAVQEFWVAVDVDDEERVVGCGALHVMWEDLAEIRTVAVDPACRGLKIGHRLVAELLDVARELGVARVFCLTFETRFFGSFGFTEIDGAPVPHGVYEQLLRSYDEGVAEFLDLERVKPNTLGNTRMLLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRD1</accession>
    <name>U5VRD1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GDSL-like lipase/acylhydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358903.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05415"/>
    <dbReference type="EnsemblBacteria" id="AGZ39372">
        <property type="protein sequence ID" value="AGZ39372"/>
        <property type="gene ID" value="AFR_05415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1097"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050180_0_2_11"/>
    <dbReference type="OrthoDB" id="1247435at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH_hydro" evidence="1">
        <location>
            <begin position="64"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="27284" checksum="FF63570841B4BFBE" modified="2014-01-22" version="1">MTSLAGRIGRATATTLFAGAVGGVALLAGEAIAARTRRYAKPTMGLALRTSMGPSAAPPLRLVLLGDSAAIGVGVEWLSETVGGQLARLLAEGTPETGQRHVLLSSVGVAGSRSTDLATQVARALLGDRPDVAVVLIGARDATGLRSPDESATHLGDAVRRLRDAGVQVVVGTCPDLGAVRSIAPPLRQLTGWVGRRVARAQAAAVVAAGGVVVDLGDETGAVFRADAGTLCYDGFHPSADGYRVWAHALYPAVLDAAQAAGRRTTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRF1</accession>
    <name>U5VRF1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Threonine ammonia-lyase</fullName>
            <ecNumber evidence="4">4.3.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_05515</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358923.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05515"/>
    <dbReference type="EnsemblBacteria" id="AGZ39392">
        <property type="protein sequence ID" value="AGZ39392"/>
        <property type="gene ID" value="AFR_05515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1119"/>
    <dbReference type="eggNOG" id="COG1171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_1_11"/>
    <dbReference type="OrthoDB" id="1876944at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:L-threonine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005789">
        <property type="entry name" value="Thr_deHydtase_catblc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01127">
        <property type="entry name" value="ilvA_1Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="329"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="407" mass="42160" checksum="F68BAF2D4501666E" modified="2014-01-22" version="1">MTDLLELADIEAARALLTGIIKTTPLEHSMPLTRTLGVPVWLKCENQQRAGSYKVRGAYTRISRLTDAERARGVVAASAGNHAQGVALAAGLLGAKSTVFMPEGAPLPKVSATKGYGATIEYAGSTVDDALAAARDFADATGAILIHPFDHPDVIAGQGTVGLEILEQCPEVNTIVTAVGGGGLISGVAVAAKALRPDIRIIGVQAVGAAAYPPSLAAGEPRTLERSATIADGIAVMRPGVLNFAHVSKLVDDVVTVTDEDLSAALLVLLERHKMVVEPAGGAAVAALLTGKVDLKPPVVAILSGGNIDPMLLLRVIEHGLVSAGRFLRLSVRTGDQPGELARLLAEIAEQRANIVDVAHSRQNPRLSFGEVEVQLSVETRGPDHSAALISVLRSSGYSLTLLSGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRT1</accession>
    <name>U5VRT1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00410"/>
    <dbReference type="EnsemblBacteria" id="AGZ38371">
        <property type="protein sequence ID" value="AGZ38371"/>
        <property type="gene ID" value="AFR_00410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.84"/>
    <dbReference type="HOGENOM" id="CLU_2244145_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="104" mass="11301" checksum="DB545EC66538DE94" modified="2014-01-22" version="1">MDRMTKFFFGNWTTRIYLFAVAVAAVILIGGNLATGDHEPSFAGIYLIGLTAPVSVIFAPYFLLGEGWLAVAMLWLSVLAGLLLNTLLINTIVGEVGKLRTRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRX3</accession>
    <name>U5VRX3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357365.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00660"/>
    <dbReference type="EnsemblBacteria" id="AGZ38421">
        <property type="protein sequence ID" value="AGZ38421"/>
        <property type="gene ID" value="AFR_00660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.133"/>
    <dbReference type="eggNOG" id="ENOG50329XM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113711_1_0_11"/>
    <dbReference type="OrthoDB" id="1793636at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014487">
        <property type="entry name" value="DUF3151"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11349">
        <property type="entry name" value="DUF3151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017349">
        <property type="entry name" value="UCP017349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="14757" checksum="09678D74E103EBD4" modified="2014-01-22" version="1">MQNLLPEPPATRLPADAEADTALAEAAKAGTDDAFKTVAARFPAFSGAWAALAESALAEKQPVTAYAYARTGYHRGLDALRRNGWKGHGPVPWSHTPNQGFLRCLHALSLAAAEIGESDEAARCAQFLRDSDPAAADALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS16</accession>
    <name>U5VS16_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_07420</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07420"/>
    <dbReference type="EnsemblBacteria" id="AGZ39773">
        <property type="protein sequence ID" value="AGZ39773"/>
        <property type="gene ID" value="AFR_07420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1514"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_14_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="234"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="451" mass="47459" checksum="58A13F3118E59AAB" modified="2014-01-22" version="1">MSDDRPADPADGVTEQDRPVTFRDVFAVREYRAVYLSLVVNWLGDYLARAAIIVLVYQQTESVLLSAASFAVSYLPWIVGGPLLATLADRYPYRRVLIVADLTRAVPIALIALPGMNVYAILCLLFVAMLGAPPTQAARSALLPLILERRKVVTGVALNASTSQAAQVFGYVLGATLAVAVDPRLGIALDAISFVVSAALIAIGVRPRPAAAVPSGRQHLLRETGEGFRLVFGTPLLRSIALMVFALTTFTILPEGLAAGWAALFHEDHSARGFDQGMIMAAGPVGFVIGGIAVGRLLRPSTRRKLIKPFALLAPTALALTVLASNAGTVAVFVAISGIAQGGLMPTLNGEFVLALPHGYRARAYGVMQGGVQFTQCFAVIATGALAQFTSIPLVVGLWSAGGLLLMIFLVARRPQLAAVERPDSPAVEVVPATHVTPGQVQAGTAGKLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS24</accession>
    <name>U5VS24_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359314.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07470"/>
    <dbReference type="EnsemblBacteria" id="AGZ39783">
        <property type="protein sequence ID" value="AGZ39783"/>
        <property type="gene ID" value="AFR_07470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1525"/>
    <dbReference type="eggNOG" id="COG2761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087602_1_0_11"/>
    <dbReference type="OrthoDB" id="996965at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="208" mass="22529" checksum="5C030342813FDEA9" modified="2014-01-22" version="1">MSERAAVDMWFDPICPWAWMTSRWLLDVEKVRPVDVTFNVMSLSVLNAGRDLPDEYKELMTKGWGPVRVCIAAAESAGPQVLRDLYTALGTRIHLQKYELDSKLYTEALTEVGLDPALADAAGSDAWDDALTASHNAGMKPVGTDVGTPVIHAPGPVEGEKIAFFGPVMTPAPKGEAAGKLWDGVVLVAGTPGFYELKRTRDVGPIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS45</accession>
    <name>U5VS45_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357434.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01010"/>
    <dbReference type="EnsemblBacteria" id="AGZ38491">
        <property type="protein sequence ID" value="AGZ38491"/>
        <property type="gene ID" value="AFR_01010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.201"/>
    <dbReference type="eggNOG" id="COG2931">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042624_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.150.10.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001343">
        <property type="entry name" value="Hemolysn_Ca-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011049">
        <property type="entry name" value="Serralysin-like_metalloprot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00353">
        <property type="entry name" value="HemolysinCabind"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51120">
        <property type="entry name" value="SSF51120"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665262" evidence="2">
        <location>
            <begin position="32"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="422" mass="41631" checksum="02D270AB31E78A3E" modified="2014-01-22" version="1">MSVFHRKALLGLGVTAAAVASTGLFAMPAQAASAGAAKVVGSSTVQFNALMGKTNGLTITISGRTVTLNDKVAIKAGKGCKAVKGDKTKVKCTTSKKTTKFSIALGDKNDWVKNNTSVYMLTDGGSGNDSLTGGSAGDQLQGGSGNDNLYGKGGNDTIFGGSGTDASYGSSGNDRIDAGSGNDKAYGDSGNDTLLGGTGNDRVVGGTGNDRLSGNDGTDIVYGESGNDTMWGDAGNDSLNSGTGNDEVSGGAGNDSLFGDTGDDILVAGAGNDREYGAAGADILVGDAGDDQLHGESGDDIVLGEAGNDRITGGDGEDILYGKAGNDIVNGNNGDDALVGEDLSDGGIQIGSNSATDQLDGGNNLTIDGDFCLVLASGTTVNCELVGTSALSASSATATPNQAALKALLDAKSRAATAATIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSC0</accession>
    <name>U5VSC0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF3817 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_07090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359238.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07090"/>
    <dbReference type="EnsemblBacteria" id="AGZ39707">
        <property type="protein sequence ID" value="AGZ39707"/>
        <property type="gene ID" value="AFR_07090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1447"/>
    <dbReference type="eggNOG" id="ENOG50330UF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120964_1_3_11"/>
    <dbReference type="OrthoDB" id="1709780at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023845">
        <property type="entry name" value="DUF3817_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12823">
        <property type="entry name" value="DUF3817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03954">
        <property type="entry name" value="integ_memb_HG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3817" evidence="8">
        <location>
            <begin position="5"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="108" mass="12116" checksum="AE0CA716CDD2C0C9" modified="2014-01-22" version="1">MQGALTRYRIIAWIVGVVLIVLVVIGMPLKYVGDNDTVVAAVGPFHGFLYMVYLVATFDLSRRLKWPLGRMLLVMLAGTIPFFSFWAERVVTRKWAGTEVQTPPAMAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT32</accession>
    <name>U5VT32_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359682.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09145"/>
    <dbReference type="EnsemblBacteria" id="AGZ40118">
        <property type="protein sequence ID" value="AGZ40118"/>
        <property type="gene ID" value="AFR_09145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1860"/>
    <dbReference type="eggNOG" id="ENOG5033D73">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100569_0_0_11"/>
    <dbReference type="OrthoDB" id="1518891at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="239" mass="24735" checksum="2615AB94E786E49D" modified="2014-01-22" version="1">MDDPTAFLTEPAKPDDSVSNGFANPLDLFNYVSPSAWLNAAIEKLTGVDVFGWMTDWVSGDWESLWKYGDAMANLAQCMQQIGINIQTGMLQLDASWDGNASDAAYKYFSDLAAATSGQQVAIAKTQDSYHKAALGAWQLSNQLGNILQALADKAILAGIAAAAGTALIETGVGAVAGYGAAAYIVLDMLQLINDASVIINTAGTVILGLFGGVMDAAYQGGDLTAVPLPATAYSGPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTB1</accession>
    <name>U5VTB1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357918.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03430"/>
    <dbReference type="EnsemblBacteria" id="AGZ38975">
        <property type="protein sequence ID" value="AGZ38975"/>
        <property type="gene ID" value="AFR_03430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.690"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="194"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="403" mass="42567" checksum="B1AF376C6087BFBC" modified="2014-01-22" version="1">MELGGWHETRVGQAVRHHPLLCDAALGVALAIFAVIALRVGPHGETPRSPGVADVFAIAVAFLAVSLRRVRTRPALIVAAIAWTAVVGVHHERQPVLTAALVVLLFTHASRTDRRTAWITAGCVAAALYLAGVAWGGGGWWAPENLGGVAWIGMATAIGDATRSRRAYVAEVEERARRAEQSRDEEARRRVVEERVRIARELHDVVAHHIAVIKVQATGAKHILAHRPERVGPALDHISRSSDAVLKEIASVVGLLRSSENLDGDPAPATEPTRGLARLSGLLDDLAAAGLRVDHHQIGESRELPALVDLAAFRIAQEALTNAQKYGDGTARLIVAYTGDGVTLDITNPVKRENAPAGSGYGILGMRERAASTGGLLTACPGAQGLFAVHAELPSAALEPAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTP0</accession>
    <name>U5VTP0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA thioesterase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10370"/>
    <dbReference type="EnsemblBacteria" id="AGZ40363">
        <property type="protein sequence ID" value="AGZ40363"/>
        <property type="gene ID" value="AFR_10370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2113"/>
    <dbReference type="eggNOG" id="COG1946">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032690_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="PTHR11066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="31167" checksum="6A4AB47C5E30CD31" modified="2014-01-22" version="1">MGQAAVDQLLEVLDLKQIDAATFQGESPQVGAQRVFGGQVAGQALVAAGRTVDPSRFVHSLHGYFVRPGDPTVPIEFTVETIRDGRSFSVRRSTAQQHGRTIFFMSASFQVVEEGLDHHTPAPAGIPGPDETPTMRDWLARYPDRAAAFSSAPQAIDVRYIGTPGWVPPGDRDAASEQRVWMRVNGKLPDDPLLHACALTYASDLSLLDSVLSTHGEVWGPGGVIGASLDHALWLHRPFRADEWFLYDCSSPSASGSRGLASGRMFTQDGRHIASAVQEGLLRRVGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU12</accession>
    <name>U5VU12_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">rplN</name>
        <name evidence="6" type="ORF">AFR_04555</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 5">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358185.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04555"/>
    <dbReference type="EnsemblBacteria" id="AGZ39200">
        <property type="protein sequence ID" value="AGZ39200"/>
        <property type="gene ID" value="AFR_04555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.920"/>
    <dbReference type="eggNOG" id="COG0093">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_2_1_11"/>
    <dbReference type="OrthoDB" id="1799923at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_L14P_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="13316" checksum="625001256CE66F12" modified="2014-01-22" version="1">MIQQESRLRVADNTGAREILCIRVLGGSGRRYASIGDVIVATVKDAIPGAGVKKGDVVKAVIVRTAKERRRPDGSYIRFDENAAVIIKDGGDPRGTRIFGPVGRELRDKRFMKIISLAPEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU27</accession>
    <name>U5VU27_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor IF-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infA</name>
        <name evidence="5" type="ORF">AFR_04630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IF-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007073013.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="SMR" id="U5VU27"/>
    <dbReference type="STRING" id="1246995.AFR_04630"/>
    <dbReference type="EnsemblBacteria" id="AGZ39215">
        <property type="protein sequence ID" value="AGZ39215"/>
        <property type="gene ID" value="AFR_04630"/>
    </dbReference>
    <dbReference type="GeneID" id="5704894"/>
    <dbReference type="GeneID" id="67457610"/>
    <dbReference type="KEGG" id="afs:AFR_04630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.935"/>
    <dbReference type="eggNOG" id="COG0361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151267_1_0_11"/>
    <dbReference type="OrthoDB" id="2066663at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04451">
        <property type="entry name" value="S1_IF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00075">
        <property type="entry name" value="IF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="73" mass="8485" checksum="E4861C9BCB9D1E59" modified="2014-01-22" version="1">MPKKDGAIEIEGRVIEPLPNAMFRVELANGHKVLAHISGKMRQHYIRILPEDRVVVELSPYDLTRGRIVYRYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU99</accession>
    <name>U5VU99_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05030"/>
    <dbReference type="EnsemblBacteria" id="AGZ39295">
        <property type="protein sequence ID" value="AGZ39295"/>
        <property type="gene ID" value="AFR_05030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1016"/>
    <dbReference type="eggNOG" id="COG4166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_0_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665220" evidence="1">
        <location>
            <begin position="26"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="74"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="536" mass="57603" checksum="8EF37B3E4EC87A21" modified="2014-01-22" version="1">MAVGATAIALLAAGCSGGGSEDTDAASNTVVIGIGEPQHLIPSNATESSGAQVLSSLFYPLVDFDAQSKPVEVGAESVTSDDNKVWTIKLKSGFTFSNGEPVIADNFIDAWNYGAYGPNAQGASYFYERIEGYADLQSVDPDGEEGPKKAPEPKAKTLTGLKKVDDTTFTVTLSAPFAGWESVMGYTAFYPLPKAAFSAPGVIADGFEDAIIGNGPFKMKGTWEHDSQIVVEKVADFKGTVPKIDGVTWKIYQDQKAEYADLTAENVDVQTTIPIESLAAAPGDLGDRFQKSPSSSFAFVGFPTFQKEFAKKEVRQALSMAVNRQEMTDQIFLGSQTPATSFVSPVVAGYRDNTCGDNCKYDPAKAKALYDANGGPKKIQVTYNADGGHKAWVDAMCNQIKASLGIECTGVGEPKFADLLTKVEKKEPVGLIRLGWLMDYPLMENYLGPLYGTGGSSNYYGYSNPAFDSLVKEGSAAKTTDEAIAKWQQAEDILAQDMPVIPLRFGQNVFGHSQKVKNVEVDLFSKVDLYKIEVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUA7</accession>
    <name>U5VUA7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative M23-family peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05080"/>
    <dbReference type="EnsemblBacteria" id="AGZ39305">
        <property type="protein sequence ID" value="AGZ39305"/>
        <property type="gene ID" value="AFR_05080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1027"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_12_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M23" evidence="2">
        <location>
            <begin position="86"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="203" mass="21428" checksum="EF4AAD9772DD3EF8" modified="2014-01-22" version="1">MVALGAASELPDAKAVNPTVLQNLNVAADGGDALAERSADGDRASRGEERAANTKISDEDAEAEAWLLPLDDYEFTSAYGVRFGKLHAGIDLAAPEGTPYKAVHAGKVKSAGYMGGYGYAVTVEHSDGTEMIYAHSRRLLVKTGDVVKAGQVMGEVGNTGYSYGTHLHLEVHVGGNPTDPIVFLREHGVDIKLRIESVYGIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUV9</accession>
    <name>U5VUV9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetyltransf_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FemABX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360669.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11725"/>
    <dbReference type="EnsemblBacteria" id="AGZ40634">
        <property type="protein sequence ID" value="AGZ40634"/>
        <property type="gene ID" value="AFR_11725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2388"/>
    <dbReference type="eggNOG" id="COG5653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_825458_0_0_11"/>
    <dbReference type="OrthoDB" id="891566at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016755">
        <property type="term" value="F:aminoacyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044038">
        <property type="term" value="P:cell wall macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038740">
        <property type="entry name" value="BioF2-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003447">
        <property type="entry name" value="FEMABX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13480">
        <property type="entry name" value="Acetyltransf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51191">
        <property type="entry name" value="FEMABX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyltransf_6" evidence="2">
        <location>
            <begin position="157"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="37624" checksum="85C41E300A3B9728" modified="2014-01-22" version="1">MLRLQRVEPTPALWADRASYEDRLIFHTEPWLRFVAQAQRAEPVLATISDGSRTVGHFTGLLTKRYGLRILGSPMAGWTTSYLGFNLDPAVPRREALQALMPFAFDDLGAAHLEIRDRGLAETDLEGLGLRWAAAPTAVIDLSPSEDALFAAMASACRRNIRKADKSGVVIEEVPGDDPTFADDFYSQLRDVFAKQNLVPTYSIERVRTLIRSLGPAGSILLLRARDADGRCIATAIMPWYHRTMYFWGGASYREHQQVRPNEALIWYALRWARERGVTEFDFVGGNSYKAKYGTTDVPVPWARRSRSPLVAGLRDAAKEGFALKQRAVARLARAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUY4</accession>
    <name>U5VUY4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_11540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360604.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11540"/>
    <dbReference type="EnsemblBacteria" id="AGZ40597">
        <property type="protein sequence ID" value="AGZ40597"/>
        <property type="gene ID" value="AFR_11540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2352"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015854">
        <property type="entry name" value="ABC_transpr_LolD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220:SF654">
        <property type="entry name" value="PTHR24220:SF654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="229" mass="24832" checksum="8C797CB570ED4200" modified="2014-01-22" version="1">MTALIELTGITKVLKGQKQPRTILDGIDLSVHPGESVAIVGRSGSGKSTLLSVIGLFDRPDQGSYLLGGQEISRLKERRAAKLRSSQFGFVFQRFFLLKHLTAAQNVSMALVNGQGWLPRRDRRARVMEALEQVGIAHLAKNRPPKMSGGEQQRVAIARALVRNPQILLADEPTGALDTETGTLVIDALLEATTRGCGLILVTHDRDHAARMGRTVDLVDGVLTERVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV64</accession>
    <name>U5VV64_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transmembrane anti-sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360823.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12390"/>
    <dbReference type="EnsemblBacteria" id="AGZ40764">
        <property type="protein sequence ID" value="AGZ40764"/>
        <property type="gene ID" value="AFR_12390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2515"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1081370_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041916">
        <property type="entry name" value="Anti_sigma_zinc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027383">
        <property type="entry name" value="Znf_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13490">
        <property type="entry name" value="zf-HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="zf-HC2" evidence="1">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="255" mass="26548" checksum="BE22274CBE05A849" modified="2014-01-22" version="1">MSGTQPGSSADCDEEQLAAYSAGELGPEERDRIAAHLLDCEACWRQSLTSRAVVTAVQRLWEPAPASLRADLRAAITAPAPIRRRLPGRLTAVLTASVALVISGMVVLTATQRHDAPTARPATQSPAAAGELPERVRAAIALYATTMPASDRPPAVPDLAPIGLRFSSASVIEVAGTPSTCYTYFDVNDLQVGLLVAATPWPRPTAAESVTAGAWATQSNGLTLLGGADGGGHQMLVIAQDHTIAMTAAAHLRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV95</accession>
    <name>U5VV95_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359156.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06680"/>
    <dbReference type="EnsemblBacteria" id="AGZ39625">
        <property type="protein sequence ID" value="AGZ39625"/>
        <property type="gene ID" value="AFR_06680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1365"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712963_0_0_11"/>
    <dbReference type="OrthoDB" id="1588027at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="41499" checksum="E9A32033A44668ED" modified="2014-01-22" version="1">MTAAGWIALAITVFGAFGYAGASILQAVAAKRSTSLARTLSHPLYLTGIALDVLAWAGSMVALRELAVYLVESILAGSLAITVLAARFVLKSKLRKRDVACVIVSISALTVLALSAGPQEGTPASAGLRWGLCTAAVLLTLLGRSAARTCPPGLVAAMGGLCLGGAALSGRALHLPEQAMGHPGALALAIVSEPLTWAVLIFAANGMLLYANALQRGQVGQVTAVHWIAEVTAPSAVALLLLGDSVRPGWGLVAIGAGVVTIGAAVLLATAPATGAAVRPPDALPDEPSQPSLPVSAPIRYAERIIWWGSPPVWTPPNRERVVALPTHHAPELTWTPPPRVQALWSDPHRPDADADEIPAEPVTLSELWPILLREEDRPEPVRSRQEQPRHGPWDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW08</accession>
    <name>U5VW08_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361051.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13530"/>
    <dbReference type="EnsemblBacteria" id="AGZ40992">
        <property type="protein sequence ID" value="AGZ40992"/>
        <property type="gene ID" value="AFR_13530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2747"/>
    <dbReference type="eggNOG" id="ENOG50341T1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069359_0_0_11"/>
    <dbReference type="OrthoDB" id="1297117at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="39544" checksum="16515320C73D8ED9" modified="2014-01-22" version="1">MTDELDLIGELGKAEALRPEAYQRARTALRAAMADPGTVRLLDAMSMENTTVEKASTMETTTTTRDHKFSPETQRRRRMSMKSRLGIGAGVGVAAAAAVVAVALNSSAPGAGVPGDNPPVAASAPAAVDGSAERAPLMTLAGSIKTATPPAGDGWLVKATQVHGKKTMQVIYTLYTDDHAIYTGDSVKGVKRAISKNQDQLKDGGYAPLLKAAVAAADRSPADGRTLMLKAAGDRLVGLDSAAQKTEWDKQQAAAQVIVKQKGGNFKPKPYSPQAVKAHLDNALWAYSTQALSAGDGNTEVRAGVLRLLSTIDAVSVKDSTTNGTATLTIIAGPEVFGGAGDEVLTVDAKTGMLVKDVSTVPGLPKSTTTYESSRVTAAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW12</accession>
    <name>U5VW12_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase sigma-E factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_14405</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14405"/>
    <dbReference type="EnsemblBacteria" id="AGZ41163">
        <property type="protein sequence ID" value="AGZ41163"/>
        <property type="gene ID" value="AFR_14405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2923"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="8"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="84"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="158" mass="17417" checksum="724F2ED70E6CAB88" modified="2014-01-22" version="1">MLYTARLLTAGDAHLAEDLVQTALTKLYVSWASYRRADNPEGYVRRTLVNALTDERKRVWRFGRPVAEVPEPSAPGAPTSDGDQELSQALRALPPRMRAVVIFRFVHDLDVAQTAEALGCSAGTVKSQTARALDKLRASLRLPATSIFFEHQQQGAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWB4</accession>
    <name>U5VWB4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361347.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41288">
        <property type="protein sequence ID" value="AGZ41288"/>
        <property type="gene ID" value="AFR_15030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3052"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073844_0_0_11"/>
    <dbReference type="OrthoDB" id="1989297at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="33518" checksum="3BF0F1BF0DFFED1C" modified="2014-01-22" version="1">MDEDLTRLAAEVRERELLGPRKAGITAAIKQLTSELAELGRRHTIEQRDVERLENFSLTRLLADLRGSRDEALARERAEAETARYRLAQAQDRLGALTAQLAQVEARLVATSTAPATYAGLLEEKERQVRASGAGSAGRLTELAGERGRLTAELAELTEAEGAARAAAQALGEANQLLESAHGWSTADTFLGGGGLSSMIKHSRLDDAAARAAEADRRLAVLRAELRDVDSALPSLAAQLELGGFTRFADVWLDNIFTDLSVRGQIKDGLGRLGDAAQQVGRIRAALAARIVENRTRIAELERERSALLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWT2</accession>
    <name>U5VWT2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">trpD</name>
        <name evidence="6" type="ORF">AFR_09405</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>diphosphate + N-(5-phospho-beta-D-ribosyl)anthranilate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359785.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09405"/>
    <dbReference type="EnsemblBacteria" id="AGZ40170">
        <property type="protein sequence ID" value="AGZ40170"/>
        <property type="gene ID" value="AFR_09405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1917"/>
    <dbReference type="eggNOG" id="COG0547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_4_1_11"/>
    <dbReference type="OrthoDB" id="1238435at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="PTHR43285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="1 3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycos_trans_3N" evidence="5">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_3" evidence="4">
        <location>
            <begin position="81"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="97"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="115"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="354" mass="36472" checksum="A5E8F1CF89D54D0F" modified="2014-01-22" version="1">MGARTWPNLLSALLRAEELSAQDTAWAMGEIMAGNATPVQVAGFAVALRAKGETPGELSGLVQAMLANATVVDLPEDVRADSVDVVGTGGDRAHTVNISTMAAIITAASGVKVVKHGNRAASSACGTADLLEYFGIPLDLGPEGVKRTVAEAGIGFCFAARYHPGMRHASVTRRELGHPTFFNVLGPLTNPARPRAAAVGCFDQRMAPVMAEVFAGRGDSALVMRGEDGLDEFTTAAPTRVWLVREGTVTESVVDAVDLGLPRSEPGDLRGGDAAFNADAALRMFAGEKGPIRDAVLVNVAAALGAHAGFPGEFADTMRAGIARAAETVDSGAATALLGRWVEAAKAAKAAETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXN7</accession>
    <name>U5VXN7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidoglycan-binding domain 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361960.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17325"/>
    <dbReference type="EnsemblBacteria" id="AGZ41743">
        <property type="protein sequence ID" value="AGZ41743"/>
        <property type="gene ID" value="AFR_17325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3513"/>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057459_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665458" evidence="1">
        <location>
            <begin position="24"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="2">
        <location>
            <begin position="121"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="345" mass="35476" checksum="AF028CDEF2324B6F" modified="2014-01-22" version="1">MSRRARTVAVAAAAALALGGAAAVAVTLDDRSSGTPAADLPPATTTVTRQTLATSVTVDGELGFGTPVPVVSKATGTVTWLPKAGAVVRRGGTLLRADDRPVVLLFGILPMYRQLSTGAEGPDVEQLERNLHKLGYDGFTVDDEFTALTAKAVRRWQKKLGLRETGTVDPAWAVVANGAIRVAKLTVRLGAPATGEVFSGTGAASVVTADVDADAADWAREGARVSIALPDGKKIAGQVTFVGTKADPKEGETPTVPVTISLAGRAPGRLREAPVDVTHTGEQRKDVLTVPVAALLALAEGGYGLEVVADGTTRYLPVEVGLFADGRAEVRADGLTEGLTVGMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXR1</accession>
    <name>U5VXR1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ankyrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361784.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16880"/>
    <dbReference type="EnsemblBacteria" id="AGZ41654">
        <property type="protein sequence ID" value="AGZ41654"/>
        <property type="gene ID" value="AFR_16880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3424"/>
    <dbReference type="eggNOG" id="COG0666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1000517_0_0_11"/>
    <dbReference type="OrthoDB" id="1694085at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="27316" checksum="B4815ACCF0F80E7A" modified="2014-01-22" version="1">MKEPTELEQLVSLVARNPGEAARRLRERSDRTPGTGPLSHLAMLRFDAARHGVTGPLTGAGALARTLIESGTPVDGLPEDPETPLITAASYGDAEVARVLLDAGADLDSTASPDAGGVRGGTALLHAAVFGMTAVLDLLLAAGARVGSLSEAAAAGTVTGWLTADTPPLDRVRALAMAADHERLAVIDQLLEARTPIDAEDTHYRRQALRLAATRGRTRSVAHLLSRGADPQHRDPFTHRTALEWCRELGGGTAVEALLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXV8</accession>
    <name>U5VXV8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase U61, LD-carboxypeptidase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_17750</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S66 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362129.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17750"/>
    <dbReference type="EnsemblBacteria" id="AGZ41828">
        <property type="protein sequence ID" value="AGZ41828"/>
        <property type="gene ID" value="AFR_17750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3599"/>
    <dbReference type="eggNOG" id="COG1619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034346_3_1_11"/>
    <dbReference type="OrthoDB" id="952832at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027461">
        <property type="entry name" value="Carboxypeptidase_A_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027478">
        <property type="entry name" value="LdcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040449">
        <property type="entry name" value="Peptidase_S66_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040921">
        <property type="entry name" value="Peptidase_S66C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003507">
        <property type="entry name" value="S66_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30237">
        <property type="entry name" value="PTHR30237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02016">
        <property type="entry name" value="Peptidase_S66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17676">
        <property type="entry name" value="Peptidase_S66C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028757">
        <property type="entry name" value="LD-carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141986">
        <property type="entry name" value="SSF141986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S66" evidence="4">
        <location>
            <begin position="17"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S66C" evidence="5">
        <location>
            <begin position="179"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR028757-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="32015" checksum="5CCA6E5577156602" modified="2014-01-22" version="1">MIPAIVRPPRLRPGDHVRLVSPASTPAPEAVEGTSRFLEGLGLRVTVGEHALDRLGYLAGTDEDRLADLNAALRDPGITAVIATKGGKGAYRIAGDLDFAAAARHPKLLVGFSEITVLHLALWQRTGVAGLHGAPFEVSWAGERAAQSFLTAAFTTDDITVTASADEPTSALTTSGRVSGVLLGGNQDMVATAAGWALPDLTGAILLLEAYNLRLGHIDRQLTMLERSGRLEGVRGIAVGQYTECGPTPDEQLDWTAVDVLRDRLGRLGVPILGGLPIGHGSDPVAVPVGTPAVLDAGAGTLTVSAAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXY8</accession>
    <name>U5VXY8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucosyl-3-phosphoglycerate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17355"/>
    <dbReference type="EnsemblBacteria" id="AGZ41749">
        <property type="protein sequence ID" value="AGZ41749"/>
        <property type="gene ID" value="AFR_17355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3519"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053119_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="38"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="35610" checksum="E683B4DA4D23F07C" modified="2014-01-22" version="1">MAPVASPVVEAWTTYRTGNAADWTARGLARAKKGDRVSVVIPARNEEATIGAIVSTIRRELVDRVSLVDEILVVDSRSTDTTAAVAQAAGARVVSQDEVTRGLPRMDGKGDALWAGLAASTGDIVAFVDGDLREFSSHFVTGLLGPLLTDPSVEFVKGFYHRPLVSTTGVEADGGGRVTELAARPMLNLFWPDLAGMVQPLAGEYAGRRRTLERIPFVSGYGVEIAMLIDLLDLVGLDALAQVDLGERFHRHQNTEALGRMSAQIMVTAWSRLHRRGLVTEPMPPSSLLTQFRRGGDALPHLDREIVVTDIGVQERPPLATLPRPVAVPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY04</accession>
    <name>U5VY04_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ThiJ/PfpI domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362012.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17455"/>
    <dbReference type="EnsemblBacteria" id="AGZ41769">
        <property type="protein sequence ID" value="AGZ41769"/>
        <property type="gene ID" value="AFR_17455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3539"/>
    <dbReference type="eggNOG" id="COG0693">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_44_1_11"/>
    <dbReference type="OrthoDB" id="1459004at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="1">
        <location>
            <begin position="101"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="22401" checksum="C25E88741DAC7C8A" modified="2014-01-22" version="1">MDTQIVLYDGFDPLDVVAPYEVLTAGSDAAGGGPRVELVSAEGARDVVSGNRGLTLRATSVLDPDRPGFVVVPGVCGPVAGDPDAGVATIPVLLARFGTTAAVPLMRRALDNPEVTVATVCGGSLALAMAGLLEGRYAVTHAQGMDMLEATGVNAVPARVVDDGDLISGGGVTSGLDLAFHLLDHAYGPRIAHAVELLFQYDRRGTVWANTGRTPVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYB1</accession>
    <name>U5VYB1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beta-xylanase</fullName>
            <ecNumber evidence="4">3.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18005</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-xylosidic linkages in xylans.</text>
            <dbReference type="EC" id="3.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362230.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18005"/>
    <dbReference type="EnsemblBacteria" id="AGZ41879">
        <property type="protein sequence ID" value="AGZ41879"/>
        <property type="gene ID" value="AFR_18005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3653"/>
    <dbReference type="eggNOG" id="COG3693">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020161_6_0_11"/>
    <dbReference type="OrthoDB" id="654705at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="PTHR31490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00134">
        <property type="entry name" value="GLHYDRLASE10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665340" description="Beta-xylanase" evidence="5">
        <location>
            <begin position="26"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="GH10" evidence="6">
        <location>
            <begin position="41"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="360" mass="39996" checksum="9A1B1127B2AED47C" modified="2014-01-22" version="1">MRVHKAWSAAALASLALLAGSPAVAAEDPAAAAVKPASPREQSLRVLADRAGVRIGTAVDTTALAADAPYRETIEREFSSVTPENVMKWQLVEPQQGVYDWAAADRLVDFARANGQTVRGHTLVWHSQNPDWLTEDAFTPAQLRALLRKHVIDQVTHFKGRIWHWDVANEIFNDDGTWRDTIWLRNLGKGYVADAFRWARQADPQAKLFLNDYNNEGVNAKSDAYYRLVRELKAQRVPVDGYGIQGHLAVQYGVPGDTLANLRRFEALGLQTAFTEVDVRMPLPADPIKVQAQAQGFTALLQACLLARRCVSYTLWGFTDRYSWVPDVFDGEGSATPLDADLVPKPAWFAMRDTLTLAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ83</accession>
    <name>U5VZ83_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PDGLE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_19535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362551.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19535"/>
    <dbReference type="EnsemblBacteria" id="AGZ42179">
        <property type="protein sequence ID" value="AGZ42179"/>
        <property type="gene ID" value="AFR_19535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3964"/>
    <dbReference type="eggNOG" id="COG0310">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137910_0_0_11"/>
    <dbReference type="OrthoDB" id="2022748at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025937">
        <property type="entry name" value="PDGLE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13190">
        <property type="entry name" value="PDGLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666229" description="PDGLE domain-containing protein" evidence="6">
        <location>
            <begin position="28"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="PDGLE" evidence="7">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="115" mass="12075" checksum="72815FBD6E45431A" modified="2014-01-22" version="1">MSKRLGWFLAGGLLVALLLAGVVSNFASGSPDGLDATAREGCTFNAQGEITGGTCMLQKEQDHQLSDSPLADYGIKGIDNEYLSTGLSGVLGVLITFAIGGGVFWLVRRRTPEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZK3</accession>
    <name>U5VZK3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19650"/>
    <dbReference type="EnsemblBacteria" id="AGZ42202">
        <property type="protein sequence ID" value="AGZ42202"/>
        <property type="gene ID" value="AFR_19650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3988"/>
    <dbReference type="eggNOG" id="ENOG5032F3C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1902177_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="133" mass="12398" checksum="5626FB1E1C409D85" modified="2014-01-22" version="1">MIVTGAGPQSKVITPPAATALTTAAEVQLAAVPVPMTLSGLLVSTALASAGTEAFPPGLPGLGRAATLAGDDDDGTADGAGEEGRALGAAGAGASDGATKAGAGAAGGGGDDPHPASSSAQLNTEILECTGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZK8</accession>
    <name>U5VZK8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362579.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19675"/>
    <dbReference type="EnsemblBacteria" id="AGZ42207">
        <property type="protein sequence ID" value="AGZ42207"/>
        <property type="gene ID" value="AFR_19675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3993"/>
    <dbReference type="eggNOG" id="COG0500">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065741_0_0_11"/>
    <dbReference type="OrthoDB" id="1515497at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="63"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="30037" checksum="E03EE56DF00AC561" modified="2014-01-22" version="1">METGDRRLMAAIEANWDERTPIHLASRFYDVEGRDPAAWFAAYEWEDLGDLTGRDVLHLQSHLGTETAAFVRRGARTVTGLDLSGAAVDAARRLAADAGLEIEYVHANVYDAREALGDRRFDVIYTGKGALCYLPDLREWAAVLAGLLRPGGQVYVVEFHPVLTGLGLVPRGDDDTLALRNDVIGGRGPVTLDATRTYTDGPELVEATTSYEWAHGLGEVVTALTGAGLTVRSLRESDELPWPRWPQMEQAASGWWRLPAPKLPLIYALRATLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZL4</accession>
    <name>U5VZL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phenylacetic acid degradation NADH oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_20830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362810.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20830"/>
    <dbReference type="EnsemblBacteria" id="AGZ42438">
        <property type="protein sequence ID" value="AGZ42438"/>
        <property type="gene ID" value="AFR_20830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4223"/>
    <dbReference type="eggNOG" id="COG1018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_14_1_11"/>
    <dbReference type="OrthoDB" id="1885467at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011884">
        <property type="entry name" value="PA_CoA_Oase5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02160">
        <property type="entry name" value="PA_CoA_Oxy5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="5">
        <location>
            <begin position="263"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="38168" checksum="882680A07B614154" modified="2014-01-22" version="1">MVVREFHALRVAAVERLCDDAVAVTFDVPDELAERYAFRPGQSLTVRRIEAGADERRTYSICAAAGERPRIGVREVPGGLVSSWLVHGLRPGDSVEVAPPTGTFTPELEAAGRHVLIAAGSGITPVLSIAASLLRHPDAHVSILYGNRRADTVMFAEELADLKDAHPSRLELVHLLSREAREVDLLTGRLDAGKLRTLLPLLIDVRGTDHWWLCGPFGMVTDATEVLSEYGVEASRIHRELFWVDEAPPEPLREERGPEGPSSEVTVVLDGRATTVTVPEGDTVLEGAQRSRPDLPFACKGGVCGTCRARVTEGDAEMRRNFALEPAEVAAGFILTCQSVPTSPKLTVSFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZQ1</accession>
    <name>U5VZQ1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14565"/>
    <dbReference type="EnsemblBacteria" id="AGZ41195">
        <property type="protein sequence ID" value="AGZ41195"/>
        <property type="gene ID" value="AFR_14565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2958"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666243" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="35"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="27904" checksum="D0B45B9AFAA47C2D" modified="2014-01-22" version="1">MRRKLFAGLAALTLAVGVSLTVATQAEAHRTKPTIVFVHGAFADASGFGASIKALQKLGYPVMAPANPLRGLAGDSAYVKSVISQIPGPIVLVGHSYGGAVITNAATGNPNVKSLVYIAAYALDEGEAVFQANSLGGGHSDLLENVEYRTFPGAGPLPPEAGGGVDADAYIKQSSFRQIFAADVPASEAALMAAAQRPAAVSSLLYPSGPPAWKSVPSWYLVAKDDKAIPPEAERFMARRAKAHTIEISSSHAAMVSNPKAVTGLVLAAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W069</accession>
    <name>U5W069_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362970.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21640"/>
    <dbReference type="EnsemblBacteria" id="AGZ42598">
        <property type="protein sequence ID" value="AGZ42598"/>
        <property type="gene ID" value="AFR_21640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4386"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035614_3_0_11"/>
    <dbReference type="OrthoDB" id="1439085at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="39563" checksum="FCFA8B4E095F771B" modified="2014-01-22" version="1">MESDGWADERFGVVADTFARNFTDFDEDGAAVTVYAGGRKVVELWRGEGWQQDTVVPVFSCAKAIVSICVHLLAQQGRLDLDAPVSDVWPEFGKPRITPRMVLGHRAGLPVLDRTLTFAEVTSWTPVIRAIEEQEPLWEPGTAYEYHAHVYGFLLGELIRRVTGLTPGAYFRQAIADDLGLRAWIGLPEAEFPHLARLSEAAGRPPLPGPETLLMRMVTMNGAFAFPGLGEPHGWNDPELLAAEIPGAGAVASATGLAGLFAAAVTGIDGSPRLLSPETVTGAVREQSAGRTWSGMDSPARWGSGFLLDAAGFRPLLGERSFGNDGAGGQFAFGDDEYGIGFAYVASRMVGYGDARATRLIAAVRESLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0C7</accession>
    <name>U5W0C7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362916.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21370"/>
    <dbReference type="EnsemblBacteria" id="AGZ42544">
        <property type="protein sequence ID" value="AGZ42544"/>
        <property type="gene ID" value="AFR_21370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4335"/>
    <dbReference type="eggNOG" id="ENOG502ZR0K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375647_0_0_11"/>
    <dbReference type="OrthoDB" id="2006941at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="20660" checksum="23D7C7F3669202C6" modified="2014-01-22" version="1">MSDEHGWPDFHDPGGHDDAGDHHDLESQHEAPIADDSPFHDDLFGHDDDPGVHDTAGEADAGVHEDAGWDDLHEPGSGHDDEAVQHSFDGAADDHGLGPVGADPDATPEAEVVSLFPPVVDVGPLPEPVDGFPWIDTGSLGLYHSGLVDPAAAAPVPAEDLAAYAGEDLPPGEDPWAALADSDDPATSTLAKWWSQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0J6</accession>
    <name>U5W0J6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CopC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_16140</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16140"/>
    <dbReference type="EnsemblBacteria" id="AGZ41510">
        <property type="protein sequence ID" value="AGZ41510"/>
        <property type="gene ID" value="AFR_16140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3275"/>
    <dbReference type="eggNOG" id="COG2372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1222619_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007348">
        <property type="entry name" value="CopC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04234">
        <property type="entry name" value="CopC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665551" description="CopC domain-containing protein" evidence="4">
        <location>
            <begin position="26"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="CopC" evidence="5">
        <location>
            <begin position="26"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="23643" checksum="54A9B33AD67FA7C2" modified="2014-01-22" version="1">MGRVFVAVLAGLLAGAVLAPGPALAHGGLAVTAPAGGSTLADPVETVSLTFTEKPSPFAYFTVTSPTGERVDAGWSNAEPVKLAKPVQEYQLTDGEWKPLLYSTGFPVKVGVSHWPALGEYVVRYHTVASDGDVVKGDVKFSYSGRSTPAPAGWQAPADQPKPELLAAAAHESAPAQAQAQAAPQTRQASDDTSIWVWLVPLLLVAAAGLIFLIVRPPSRRTKPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0T9</accession>
    <name>U5W0T9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alpha/beta hydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363384.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23015"/>
    <dbReference type="EnsemblBacteria" id="AGZ42873">
        <property type="protein sequence ID" value="AGZ42873"/>
        <property type="gene ID" value="AFR_23015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4664"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_3_1_11"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="232" mass="24553" checksum="DDC4E5917EAD4B90" modified="2014-01-22" version="1">MHFVFIPGAWHGGWSWHPVGHRVREAGHGATALTMPGLSLGDDPAGLSLDDAVDHIVTAVECRDLREVVLVGHSWGGIPITGAAHRLRDRLAGISYFSAFIPRRGESMARAMGPMEGFLTDTIAASPDRTIGLDFPTFQQGLMPGEPEAVQRLVYDQLMPQPGGYMLDALDLPGVETLGLPITYVLAENDISLAAPGTELAARVGVQPVLVPGGHEALLTSPDEVAKALLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W123</accession>
    <name>U5W123_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_23440</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363539.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23440"/>
    <dbReference type="EnsemblBacteria" id="AGZ42958">
        <property type="protein sequence ID" value="AGZ42958"/>
        <property type="gene ID" value="AFR_23440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4750"/>
    <dbReference type="eggNOG" id="ENOG5032TP9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126433_1_1_11"/>
    <dbReference type="OrthoDB" id="2025483at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13564">
        <property type="entry name" value="DoxX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="112" mass="11633" checksum="2294321992A70C98" modified="2014-01-22" version="1">MSAVVTVVAAVLTGFTAVVYLIGHDYPKEQARMKRLPPEWVPRLGLALAAGSLGLLVGFVVPPAGILAAAGLVLYFVGAFVAHLRVGSRQLAGPAVFLVTSVAALVVQIGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W125</accession>
    <name>U5W125_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GDSL family lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361786.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16885"/>
    <dbReference type="EnsemblBacteria" id="AGZ41655">
        <property type="protein sequence ID" value="AGZ41655"/>
        <property type="gene ID" value="AFR_16885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3425"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069365_1_0_11"/>
    <dbReference type="OrthoDB" id="1247435at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH_hydro" evidence="1">
        <location>
            <begin position="5"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="27466" checksum="55CDA5CBB15EC0E5" modified="2014-01-22" version="1">MRYVAVGDSFTEGMGDELPDGSVRGWADLVAAGLASAEPGSVQYANLAIRGRLLDPIVNDQLEAAMALSPAPTLVTLNGGGNDMMRRGTDIERLVGLLDRAIRRCVDAGIRMVVLSGADPSERLPFGGVIQRRAEILTTRTRELVEKYDIELVDVFHDVEIRKAPYWSADRLHLNAAGHQRVAGLVLTQLGHDHAAHVVDPGPGESRRVLAEARYYREHVLPWVTRRLRGRSSGDDRTGKYLDWVPVETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W179</accession>
    <name>U5W179_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acyl carrier protein phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363410.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23145"/>
    <dbReference type="EnsemblBacteria" id="AGZ42899">
        <property type="protein sequence ID" value="AGZ42899"/>
        <property type="gene ID" value="AFR_23145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4692"/>
    <dbReference type="eggNOG" id="COG1182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088964_0_1_11"/>
    <dbReference type="OrthoDB" id="1402654at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003680">
        <property type="entry name" value="Flavodoxin_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02525">
        <property type="entry name" value="Flavodoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin_2" evidence="1">
        <location>
            <begin position="4"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23172" checksum="40989B3E73791C9D" modified="2014-01-22" version="1">MSTVFRLDSSIRGEGSVTRAVADTLESALVADLDGAAVLRREIGLTPLDAAAWGLSAFAGYVPEDQRSPEQVKAMALATELADELVAAEAYVFAVPFYNFGVSQHVKAWVDLLMTEPRFAPGTQSIAGRPAFLVIARGGGYGAGTPREGWDHATAWLTRVFSDIWGLDLQIIESELTLADVNPAMAELRELAATNLEEAHNAAHKHGKRLAVVLSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Q0</accession>
    <name>U5W1Q0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24360"/>
    <dbReference type="EnsemblBacteria" id="AGZ43138">
        <property type="protein sequence ID" value="AGZ43138"/>
        <property type="gene ID" value="AFR_24360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4935"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122336_0_0_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="49"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="17470" checksum="DEE82283B58F990E" modified="2014-01-22" version="1">MTEPDLLDSSYYGPLFRLLADMDNDIAGLYAEPRFEGIRTRFVGPLIDLGRRGPLTIRELADARKVTHSAMSQTATAMRKAGFVEPAEGTDGRTRKVQLTGRSRDLVPFLEAEWRATEKTVRDLDAELAYPLMKAVADVREALAARSFAQRLRDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2A5</accession>
    <name>U5W2A5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43264">
        <property type="protein sequence ID" value="AGZ43264"/>
        <property type="gene ID" value="AFR_24990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5062"/>
    <dbReference type="eggNOG" id="COG1621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_983302_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="33002" checksum="CF4EF99C8AF9302C" modified="2014-01-22" version="1">MSIGTQALPLPRWEDATVVVEPPGDEPGAWAGAPSSIVADGDVYLAYRLRLPIGEGRGISNVVARSTDGLTFTVVAEVSKDTFGAESLERPALVRTPEGRWRLYVSAATPGTKHWRVDLLEAATPEGLATATPRTVLAGDDTIGVKDPVLHHDEHGWHLWASVHPLESWDDADRMTTEYATSPDGIHWTWRGTALAGRPGRWDARGVRISSVSVDGDTIVAAYDGRATAGENWEERTGTARGLRLPDGSFGELTAEDGEPVGSPHAPNGLRYLSLVTHDDGRRRIYYEATREDGAHDLRTELAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Q4</accession>
    <name>U5W2Q4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fatty acid desaturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363882.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25130"/>
    <dbReference type="EnsemblBacteria" id="AGZ43292">
        <property type="protein sequence ID" value="AGZ43292"/>
        <property type="gene ID" value="AFR_25130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5092"/>
    <dbReference type="eggNOG" id="COG3239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045383_0_0_11"/>
    <dbReference type="OrthoDB" id="1032884at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="PTHR19353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="FA_desaturase" evidence="2">
        <location>
            <begin position="72"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="46122" checksum="F48145BC6AE12A86" modified="2014-01-22" version="1">MTTIQRKRVSPIDHLTAEDIETLGRELDAIRDEIVAGRGENDAKYIRRVISVQRKLELSSRAILLFSLFPPAWLVGTAGLSVAKILENMEIGHNIMHGQWDWMRDPKIHSTAWEWDNASPSEQWKHSHNELHHTYTNVVGKDNDLGYGIMRVDEGQRWHPIYLGQPLWNFVNACFFEYGIAAYDLELGKNLKTKKRRTNPEFKERLKQVRRKIGKQMLKDYVVHPALSIVTGSFLHTLAANAVANLVRNLWTHSVIMCGHFPQGVETFERTSIEGETRGEWYVRQMLGSANIDGNRVMHIMTGNLSYQIEHHLFPDLPSNRYQEIAPRIREIFDRYQLTYVSGSLPRQVGSAWAKVFRLSLPNRGEAKLAADAPRPKQVKRAKAYRWQRPAEEPTPVAVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2S8</accession>
    <name>U5W2S8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364327.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26355"/>
    <dbReference type="EnsemblBacteria" id="AGZ43533">
        <property type="protein sequence ID" value="AGZ43533"/>
        <property type="gene ID" value="AFR_26355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5342"/>
    <dbReference type="eggNOG" id="COG2378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041141_2_0_11"/>
    <dbReference type="OrthoDB" id="1654295at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028349">
        <property type="entry name" value="PafC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043839">
        <property type="entry name" value="PafC_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19187">
        <property type="entry name" value="HTH_PafC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016838">
        <property type="entry name" value="PafC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_PafC" evidence="2">
        <location>
            <begin position="15"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="WYL" evidence="1">
        <location>
            <begin position="149"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="328" mass="36135" checksum="B733345626F804CE" modified="2014-01-22" version="1">MTPQPRTRENKASADRLGRLLNLVPYLLARPGILVAEAAAAFGITERQLREDLELLWVCGLPGYGPGDLIDMALTDDRVTITYDAGIDRPLRLNPDEALALVVALRMLAETPGIGSRDAIERALAKIESAAGDLADTPVAVRLPANVEKVDAIRGVVERRHALRLTYYTAARDETTDRVVDPMRMLMVGPRAYLEAWCRRAEGTRLFRVDRIDAFTELDEPSAPPAQAVPHDVSDGIFHPTPDLPLVTLRVGRGSRWITEYYPCEEVRRDGDEWIVTMRVTDLGWAQRLLLGLGSDVTVVGPVELVDRIREQASTALDQYAAPAPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3D2</accession>
    <name>U5W3D2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DEAD/DEAH box helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362877.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21165"/>
    <dbReference type="EnsemblBacteria" id="AGZ42505">
        <property type="protein sequence ID" value="AGZ42505"/>
        <property type="gene ID" value="AFR_21165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4290"/>
    <dbReference type="eggNOG" id="COG4581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017075_0_0_11"/>
    <dbReference type="OrthoDB" id="66530at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021904">
        <property type="entry name" value="DUF3516"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12029">
        <property type="entry name" value="DUF3516"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="39"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="219"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="832" mass="92022" checksum="E3A990CA9AC93EE3" modified="2014-01-22" version="1">MTLTERLPASAEPDAVFDAFQTWVAEQGLSLYSHQEEALIEIATGANVILNTPTGSGKSLVATGAHFMALADKRTTFYTAPIKALVSEKFFALCRIFGAHNVGMLTGDANVNSDAPIICCTAEILANLALREGAAADVGQVVMDEFHFYAEPDRGWAWQVPLIELPQAQFILMSATLGDTTRFVDDLARRTGRPTAVVSNTERPVPLIFQYAMTPLHETIEELLTTKQAPVYIVHFTQASALERAQSLMSINMCTRAEKDAIAAAIGSFRFTAGFGRTLSRLVRHGIGVHHAGMLPKYRRLVETLAQAGLLKVICGTDTLGVGINVPIRTVLFTGLSKYDGVRTRLLKAREFHQIAGRAGRAGFDTVGTVIVQAPEHVIDNERMLAKAGDDPKKRRKVVRKKPPEGTIGWGQPTFDRLVAADPEPLTSSFGVSHAMLLNVLARGGDPFAAMRHLLTDNHEEPAAQRRHIRRALAIARALLAGGVIERVVTPDGVRYSLTEDLQLDFALNQALSPFALACLELLDKESPEYPLDVVSVIEATLEDPRQVLSAQQFKAKGEAVNAMKAEGIEYDQRMELLEEVTYPKPLLPLLEGAYETYRQGHPWVADYEIKPKAVVRDMYERAMTFTEYVSFYGLSRSEGLVLRYLADAFRALRQTVPEDARTEELTDLIEWLGELVRQVDSSLLDEWERLRNPGAEVVEVSPLDDKPPAVTRNVRAFRVLVRNAMFRRVELFALRRAEDLGELDAESGWNTDRWIEALEDYYAEYPVVGTGPSARGPALLQIETTPAMWKVRQIFEDPEGDQDWGIEAEVDLEASDEIGAAAIRIIAVGPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3P1</accession>
    <name>U5W3P1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative RNA polymerase ECF subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_27885</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560175.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27885"/>
    <dbReference type="EnsemblBacteria" id="AGZ43838">
        <property type="protein sequence ID" value="AGZ43838"/>
        <property type="gene ID" value="AFR_27885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5651"/>
    <dbReference type="HOGENOM" id="CLU_1912570_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="93"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="132" mass="14672" checksum="372A0B6D35D2CB31" modified="2014-01-22" version="1">MDTVVFLPEYRREPARLLRLLRRAAAADRDAFTRLYQALAPDVDAAVHVTLGGERADEVAAATFLEAWQSADLHTAPGTDVAGWIIGIAARRAGEQDDPRVRSHPSQHTYSPGETLAGLLQRRDTRRSPFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3S3</accession>
    <name>U5W3S3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362928.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21430"/>
    <dbReference type="EnsemblBacteria" id="AGZ42556">
        <property type="protein sequence ID" value="AGZ42556"/>
        <property type="gene ID" value="AFR_21430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4347"/>
    <dbReference type="eggNOG" id="COG3324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1164902_0_0_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase_6" evidence="1">
        <location>
            <begin position="13"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Glyoxalase_6" evidence="1">
        <location>
            <begin position="123"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="232" mass="25233" checksum="52EC4154ECB167E4" modified="2014-01-22" version="1">MKWITGFLDTPSRVAEPFWEAITGAGLSPRRGPGGEFATLLPDDGDAYLRVQVVGDGPPRSHLDLHVEDVPAQARRVSALGATVVLDDGELVVLRSPAGLVFCLVPWHGEQVRPAGGRSLVDQMCLDLPHAVFDAEADFWSAVTGFERRPGSLPEFDFLVRPDGMPLRLLLQRVGGDEAGMHLDLACDDRPAEVARHVELGATVVRSEPRWTTLRDPAGRVYCITDRPVGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W410</accession>
    <name>U5W410_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560206.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28040"/>
    <dbReference type="EnsemblBacteria" id="AGZ43869">
        <property type="protein sequence ID" value="AGZ43869"/>
        <property type="gene ID" value="AFR_28040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5683"/>
    <dbReference type="eggNOG" id="ENOG50342T3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_859520_0_0_11"/>
    <dbReference type="OrthoDB" id="2149530at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666070" evidence="1">
        <location>
            <begin position="27"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="33624" checksum="0FC5DDB61B9BF01B" modified="2014-01-22" version="1">MRRAAQLTTIVAAAALLIVPGSPAQAYVSQVDVSPTTITDSSTGEGSIAATMHLSMQAGEARNLRAELVMGNARAEGQPSPVAAAQKITCEPAGGATLPGEQIWNTRNLLADEPDVTLMTRMMFVAPAAGSYDCHLRVYLNNELSHGRETAAMRGGFLGDVSGAIPADRTARLIRSGSDATYFALGAPVRQLHHVPGYTPPVGATSFRVVGDLQTSSCYGNGGNACPRATYPDGAAAKIYTRVVATPSIPSAACTSQASAPRSVTVSKQVHHLRIHNELLVTLPSSGCGTWTLNVVARDDGGSLPFVVHTGDGPYTTVYARPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4D1</accession>
    <name>U5W4D1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28910"/>
    <dbReference type="EnsemblBacteria" id="AGZ44043">
        <property type="protein sequence ID" value="AGZ44043"/>
        <property type="gene ID" value="AFR_28910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5860"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_28_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="21167" checksum="6B79F57535E10FC1" modified="2014-01-22" version="1">MRRGRPPTFDRAAALAAATRLFWENGYEATSINDLTRAMGIRPGSLYAAFGDKRALFNEVVEAYGRSPAGEFIWAAMAREPTAFNAFHRILLDAAAMYTDPETPAGNLLCSAAAADTDVATYMRDLRNASVATFEQRLKTAQDQGELPATAEPRKLAAYFTAVIHGMSQRARDGATAAELTDTAELAMAAWPAPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4G6</accession>
    <name>U5W4G6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_29110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560420.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29110"/>
    <dbReference type="EnsemblBacteria" id="AGZ44083">
        <property type="protein sequence ID" value="AGZ44083"/>
        <property type="gene ID" value="AFR_29110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5897"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_11"/>
    <dbReference type="OrthoDB" id="1074175at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="87"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32230" checksum="18CDF8176C5D0B12" modified="2014-01-22" version="1">MTTTQSAPSAARRPGLSGWSGRTIVAVVVTVFVLFFAIPIVWLLLATTKTARGLITSNPFAPGSAGGLSTNWSQLFGFQDGAVTTWMTNSALYAIGALIITLVASIPAGYALALTEFRFRRVLLVLTLVVMLIPNTALVLPIFLELNVVGLIGSPLSVILPMSFFPFGVYLTYIYFSTSIPRDLLAAARIDGCREFQVFTKIALPLAAPIVALVAFFSFVQNWNNFFLPFVMLPSSDGYPVQVGLTSLLASTPAFNPSSAGAQSVQLPTLALATVVSVLPVLLVFVVSQRFLVAGMTAGGTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4M5</accession>
    <name>U5W4M5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glucan endo-1,6-beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_23400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23400"/>
    <dbReference type="EnsemblBacteria" id="AGZ42950">
        <property type="protein sequence ID" value="AGZ42950"/>
        <property type="gene ID" value="AFR_23400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4742"/>
    <dbReference type="eggNOG" id="COG5520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014379_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004348">
        <property type="term" value="F:glucosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033452">
        <property type="entry name" value="GH30_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001139">
        <property type="entry name" value="Glyco_hydro_30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033453">
        <property type="entry name" value="Glyco_hydro_30_TIM-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11069">
        <property type="entry name" value="PTHR11069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02055">
        <property type="entry name" value="Glyco_hydro_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17189">
        <property type="entry name" value="Glyco_hydro_30C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666074" evidence="5">
        <location>
            <begin position="32"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="6">
        <location>
            <begin position="475"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="606" mass="63338" checksum="CCCD341669A0C82E" modified="2014-01-22" version="1">MKYAVPKRSALAVLATATTLVGIGAAVVATASTSVAATTASVWLTTPDRNNLLRQQANVVFDGGGSGSTITVNPATTYQSMVGFGASLTDSAAWNIFNSPQRSSIMNSLFGSGGIGLSWLRQPIGASDYSRNFYTYDDGSADPTLSRFSIAHDNAYILPLVTQARSLNPKLSVMATPWSAPAWMKTNNQLIGGSLSDSNIGVYSDYLVKFVQAYQAAGVPIPYLSVQNEPNFSPPGYPGMLMSAGQQVSIINTLAPKLRAAGLTTKILGYDHNWDDTSYPQTVNNGAGSNVAGSAWHCYGGNPGGQSVVRNAQPSKDIFFTECSGTESANTFADTLWWQGRNLAIGTTRNWARTVTTWNMALNAQQGPVIGSCTNCTGVVTVDGGSVTYNAEYYVLGHLSKFVQPGAVRVDSTGFGQGGIENVAFRNPDGSVALVAINTGGTQSFRVAANGSSFGYTLPAGSMATFTWPGGTSGNPPAGRTGAISGLGGKCLDVTNGSTTNGNLPQMWSCSSGPNQTWTLGTDGTVRALGKCLDLKDSSTADGATVQLWDCFGGPNQRWTYTSGRDLINVASGKCLDVRNNSTADGAQLQSWACTGAANQKWTAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4M8</accession>
    <name>U5W4M8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560406.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29040"/>
    <dbReference type="EnsemblBacteria" id="AGZ44069">
        <property type="protein sequence ID" value="AGZ44069"/>
        <property type="gene ID" value="AFR_29040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5884"/>
    <dbReference type="eggNOG" id="ENOG5030375">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177490_0_0_11"/>
    <dbReference type="OrthoDB" id="2006936at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="95" mass="10215" checksum="29DA1027AA083466" modified="2014-01-22" version="1">MPAADVTRPYGLSRPTMQDAREAMHRVHGPGGPAAWSRLLTSAGIPATNLGDADLPRLLEAMARLDPVSKLCAQALNIRLTSHTHLSAAHDLTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W511</accession>
    <name>U5W511_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dehydrogenase with different specificities</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560424.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29130"/>
    <dbReference type="EnsemblBacteria" id="AGZ44087">
        <property type="protein sequence ID" value="AGZ44087"/>
        <property type="gene ID" value="AFR_29130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5901"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_11"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="26107" checksum="25A345D1862FAD65" modified="2014-01-22" version="1">MTGLFDLSGRLAVVTGARRGIGFAMAEALAAAGADIIGVSAQLRPDDKIATTVRGHGRTFEAYQADFADREAVLALGAELAGRAPDILINNAGTIERSPAAQHPLSWWDRVIEVDLSNQFALTQLVAAPMLERGHGKIIFTASLLSFQGGINVPGYTAAKSGIAGLTRALANEWAGRGVNVNAIAPGYIATDNTAALRADPDRSRSILERIPAGRWGNGQDIAGAAVFLSSAASDYVSGTTLPVDGGWLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W517</accession>
    <name>U5W517_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Xylanase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560434.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29180"/>
    <dbReference type="EnsemblBacteria" id="AGZ44097">
        <property type="protein sequence ID" value="AGZ44097"/>
        <property type="gene ID" value="AFR_29180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5912"/>
    <dbReference type="eggNOG" id="COG3509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013864_1_0_11"/>
    <dbReference type="OrthoDB" id="1177619at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665545" evidence="1">
        <location>
            <begin position="38"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="663"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="793" mass="83136" checksum="9E82BF9EF71222F1" modified="2014-01-22" version="1">MPVTKSSLFRKKVLTAAAAAVVAAAVGVVTAASPAQAATTTIYASPSGTGSACSSAQPCSLAGAQAAVRSLNDSMSGDIVVQLADGVYRLSAPFRLTAEDSGSNGYRVVWQAAPSARPAISGSRAVTGWTQADAGRNIWRADVGAGTDTRQLYVNGAVATRARTAVNRSDFTVTTTGLRFSNGALSYLNNLADQSRIQMESIGSFTDRYASVQSISGNVITMQQPSWNNNTFGYDTFSSPHRAGPLYLTNAYELLDSPGEWYLNRGTGALYYIPAAGQNMSSISVELPTLQSLVNVGGTYDSPAHHLTFSGITFTGTSWLGASSNQGYADQQTGAYVTGNWNWPALSACQEGCTQFEATRPNWNQMPAAVQVSAADSITFSGSQFVNLGQTAIGIGNDANAHASGVGLGASNITVTGSEIARSSAGGIVAGGVRADAHHPSDQRMVNRNITISNNRIHDLGVEYRGNVSVLTTYVTTATVSHNEVYNMPYSGMSIGYGWGSNEPGGSTQYTNRGLYNYQPRYTTPTTASNNRLIGNYIHDVMQQTTDGGCIYTLSWNPGAQISGNYCLRTNGWFGLYFDEGSKYYTASGNVFSSIGTWATANYWGGENMGNFTVTGNWTSNSATNVTNGDRGNVVNTNTTVTNGSWPSGAQAVMAAAGPQTGGTPATRLVGTQSGRCADVPNSVTTNGTQTQLWDCSGAGNQNWTYTSGKQLSIYGGTKCLDANGQGTSNGTTAIIWDCSGQANQQWNVNANGTITGVQSGLCLDAVGANTANGTKLDLWSCNGGANQQWSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5C4</accession>
    <name>U5W5C4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560549.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44212">
        <property type="protein sequence ID" value="AGZ44212"/>
        <property type="gene ID" value="AFR_29755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6029"/>
    <dbReference type="eggNOG" id="ENOG503453N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1101042_0_0_11"/>
    <dbReference type="OrthoDB" id="1769944at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="252" mass="28544" checksum="47D500268B8D97EA" modified="2014-01-22" version="1">MQSVDRVLARHLAYLAFIEIRSAAGGPTRTPAKTTPAEALTHIRFLSDLCHNLPLGEGRRPDRSRTRPPSRREVAMRERPMSWTWNTAGPQGQAWILAHVAKLDLDWTPPPPLPTPYVVLPPFTLQQRMRFLARWPVGTPREQRVLKVYDNTTLAAHSPQLAGLVDPGVEHYVFPDPSPYDAQSAELLCRLLLRMVDGAEVTSHVRLAPEVFAALPSSVPFWRQRLLAHRARLVERDLGLWTRDRDRVSSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5G4</accession>
    <name>U5W5G4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase C14 caspase catalytic subunit p20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24795"/>
    <dbReference type="EnsemblBacteria" id="AGZ43225">
        <property type="protein sequence ID" value="AGZ43225"/>
        <property type="gene ID" value="AFR_24795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5023"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002396_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_repeat"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="844"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1196" mass="126262" checksum="48FD3F0E4B4F6148" modified="2014-01-22" version="1">MGGAVAAMAELSGTDDPRPITVVLWLTVAVLAFALPVTVLLTRSRQDRRRHGAEAVGELRRHFLTRGRGVTPSSFRRQWYFTGRERALRELSAWLAGELPGDHRTRVVTGGPGAGKSAVLGRVVTFGFPALRREVPAGQTPADAGLVPPPGSVHCVVHARGRTADQVAADIGRNVGRPVGTALELLAVPHRDAGRPVRGIVIDGVDEAQDPEHLITELLEPLAAGAAAWRIRLLLGLRTGADRSLLRRFGPYLLEVPLDDPGYFDRADLSEYARRCLLAEGEPGVSSPYRKRPELAARVAAAVAQRAGTNFLIVQLTCVTLAALPEATDASPEQFPDSVGAAMERYLAAFGAERRTARDLLVPLAFAQGAGLSDPVVWAALATALGTARYTEQDVAWLLGERRAVHLLTRADDASRLFHEALGEHLRAAVAERTGVRAAHRLITRTLIDHVPPGPDWLAAGKYVRLHLAAHASAAGQVTELLLDPLFLIATDPDEVIGVLPINERAEGRPSPELQDVIDVVLRAGRTLFVRNDAERAAYLQMAARKLGAEELAGRLAALPLALPWSVPWARWRTITAGAGLIGSQNEYVRRLSVGAQLIVACSDNSLGVWRLTAEGIHPVLLPERLRGLLSAAPVPGAVITVHTDGSLLRHDVVSGEVQPWTPKVGVDQLDYCAPITFQGRDLLVLGGASHVRLWDPVDDKPAGPLMQLPPGSSLLDAATCTGRPLVLTDLAGRVRAFDLAAGDPYGESFLAFDDTIADLDGPVWSGALGEADGHVVAVVAGRGDRPIVRYDLDTGGRLGGDLASPGIATMSLAVGYGLLGAGGADGRLKLWSWPAGEPIGHDVAAHEGGIDSVAVLELNRETVVVTGGRDGATRVWWPRDSAARTKDDLSTGQLLRMRVGGKERLLATTGDDLLLLDPGDGRTIATRPAPNRGRDTLGRYLGPGPGNVAITMADGTVHLIDAATLKTRRRFTAAGPDDIAALATAPGRVLVVTRTGMLQIWDPARPRPIRTVEVGPGVWNMVAINGIALAPGRDTIAAVVLDTGQPSAERLAAGCPLSSSIYRIVTGKITGRGVAVGIGASSHVHVWDATKGTLLVDTIIDDGHRMALSDAVVAPLHGRDVVVTGGYAGAIAIWDLDGRVGEVIELGSPVWSLAVVGGDTIVAGGPSGLIAITLGRDAVLTDRARRDLGVRLLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5M4</accession>
    <name>U5W5M4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetylglutamate kinase</fullName>
            <ecNumber evidence="7">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">NAG kinase</fullName>
            <shortName evidence="7">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">argB</name>
        <name evidence="9" type="ORF">AFR_30235</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560644.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30235"/>
    <dbReference type="EnsemblBacteria" id="AGZ44307">
        <property type="protein sequence ID" value="AGZ44307"/>
        <property type="gene ID" value="AFR_30235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6122"/>
    <dbReference type="eggNOG" id="COG0548">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053680_0_1_11"/>
    <dbReference type="OrthoDB" id="901370at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="63"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="30232" checksum="4331F82C6F8C2046" modified="2014-01-22" version="1">MTPHEKAATLIEALPWLERFHGTTVVIKYGGNAMIDPELQRAFAADMVFLRYAGIKPVVVHGGGPQISSMLGKLGIESEFRGGLRVTTPEAMDVVRMVLVGQVGRELVGLINEHGPFAVGLSGEDARLFTAVRRHAVVDGEPVDIGQVGDVAHVDTSAVDDLIAAGRIPVVATVAPDVDGVVHNVNADTAAAALAVALGARKLVVLTDVPGLYTNWPDTSSLTTKIDTDALEKLLPSLQSGMVPKMEACLRAVQGGVPAAHVVDGREPHSTLLEVFTSEGFGTMVVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Y4</accession>
    <name>U5W5Y4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_25485</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363988.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25485"/>
    <dbReference type="EnsemblBacteria" id="AGZ43361">
        <property type="protein sequence ID" value="AGZ43361"/>
        <property type="gene ID" value="AFR_25485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5164"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_11"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="171"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="232"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="438" mass="45209" checksum="0D40480A0379612F" modified="2014-01-22" version="1">MSLRSRLVILFAAGTSVVLALGLSLLYLALDRQLTAAVDADLRGRAADLISGIAAGDRTAVAADPMAQLFATDGALLTGTSSLPGPLLDPARVRATTTPSLVRTQLPIGYRSGPVRVRLLAEPVGATGEVLAVAVASGGVERGSDRQLTVLLVAAPVLVAVLAALGWLLVRAALRPVDALTREAAAISTLDSARRLPAVSGSDEIARLAATLDDMLGRLAVSFARERAFVDDASHELRTPVAVLRGEIDLALSALDDSAEVEQSLRAAQDQVARLARLTEDLLVLARERGGSLVVHREPVDLADLVRAEARMPVSVRGEPVVVEADADRLRQVIANLAANSTAAGATRADVRIGTETGAAVLEWSDDGPGFAPEVLASAFERFVRGDPARSAAPGSGLGLSIVRAIAIAHGGTATARNAPTDGAVVTVRLPGPFRNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W605</accession>
    <name>U5W605_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M14, carboxypeptidase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31740"/>
    <dbReference type="EnsemblBacteria" id="AGZ44608">
        <property type="protein sequence ID" value="AGZ44608"/>
        <property type="gene ID" value="AFR_31740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6425"/>
    <dbReference type="eggNOG" id="COG2866">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012481_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03859">
        <property type="entry name" value="M14_CPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033810">
        <property type="entry name" value="Carboxypeptidase_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008757">
        <property type="entry name" value="Peptidase_M6-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05547">
        <property type="entry name" value="Peptidase_M6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M14" evidence="2">
        <location>
            <begin position="102"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1004" mass="107600" checksum="9FD0E0AABC0558C6" modified="2014-01-22" version="1">MTAAATVLSGIAPAAAAPPEDTLAVYVGTLDSPQLERLRAVGIDHDESAVTAAAGGKVAVETILGKHQADRLIAAGVPLTEKRTSSAMRKQAAAAPTVFRQYNTPGGIRDELAATAARNPRLAKLITIGKTRKGVPIQGVKVTRDARIVPDGRRPAAIYLGGQHAREWITPEMNRRLLHYVLDNYGKDAALTRLVDTTELYFLPVVNPDGYDFTFTEGNRLWRKNLRDNNGDGQITPGDGVDLNRNFAEKWGYDNEGSSPDPASETYRGPSPGSEPETKALDGFFKKVQAEFLVNYHSAAELLLYGIGWQVSTPSPDDQIAIAMAGDDAHPAIAGYDPDISAELYTTNGDTDFHAQVRTGAIGFTPEMSTCETISNLYPDDQWNAADCQSGFNFPDDEKLIQDEFVKNLPFALSVAKSAQTPDNPVSVVGRSTADLTVDAFDTSYGSRQQVAVSARRSLRDVTLHYKVNGGRERTEGTREWKGGERYGDGIDKYFAERRGVVPKARAKDKVQVWFTARKGRQTVKSAPFTYTVADKIGGDVLVLAAEDVTGVSPANTDGATSARYADEHVAALKAAGKRADVYDMDLNGRKAPHPLGVLSHYKAVVWETGDDIIPRASGQPGGTVARSALETELSVRDYLNEGGKLLLSGKYAGFAQGANGAYVYKPEGPGECTDAADPTCLPIANDFQQYWLGAYSYVDDGGTNADGVPNPVVGTSGAFTGYKGTPAGDHTAAFLSTSSFLPKAEFPQFSSEAPLDWQTSGASPYDPYDGDWYLWSGQADVSYKRLTKTVDLSAATAARLKFQTSFDTEAGWDFMFVEAHEVGTDNWTTLPDAGGLTTTETGDSCQSGIETIHPFLTHYQGAECSPTGTTGTWNAATGSSNGWKQFDADLSAYAGKQVELSISYMSDWGTQGLGVFLDDVRVEIGGAVAQQTSFETDLGGWTVAGPPPGSGVNSSDWSRSQLAFDSGAGTTTADSVYLGFGLEGLTVAERNDLVRRALRHLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W642</accession>
    <name>U5W642_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain fatty acyl-CoA assimilation regulator (ScfR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25785"/>
    <dbReference type="EnsemblBacteria" id="AGZ43421">
        <property type="protein sequence ID" value="AGZ43421"/>
        <property type="gene ID" value="AFR_25785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5227"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3800">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_552960_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010359">
        <property type="entry name" value="IrrE_HExxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06114">
        <property type="entry name" value="Peptidase_M78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="471" mass="52283" checksum="5971C229CE4B9473" modified="2014-01-22" version="1">MTLGQRLRHQRRAKGLTLDQLSAVVGRAPSQLSLIENGKREPKLSVLQAIAGALGVPMQDLLRPEAPSRRAGLEIELAHFQREPAYAALGLPAVRGGRRLPADALESLIGLHRELTRLLTEQSATPELARRANQQLRVEMRERDNYFAHIEAAAAKVLQSVRHTTGPLSQRGILDIAAQLGFSLHYVNDLPGSARSVTDLRNRKIYLPQVASGKGHDPRAVVLQTLGHFVLGHNDPADYGDFLRQRVEVNYFAAALLMPEKFATDFLRDAKADRALAIDDFRDAFGVSYETAAHRFTNLVTHHFGIPVHFARVHESGTVYKAYENDDVRFPTDVTGAIEGQPVCRKWASRTVFSSDDPYSSFYQFTDTGKGTYWCTVHVESTRSGEFSVTVGTPYQHARWFRGGDTTRRTVSSCPDPTCCRRPPAELVESWAGQAWPSARVHSHLLAALPPGTFPGVDAQDVYEFLQRRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Y0</accession>
    <name>U5W6Y0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560078.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27395"/>
    <dbReference type="EnsemblBacteria" id="AGZ43740">
        <property type="protein sequence ID" value="AGZ43740"/>
        <property type="gene ID" value="AFR_27395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5553"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060397_1_1_11"/>
    <dbReference type="OrthoDB" id="1865374at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="41"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="22760" checksum="77D37D8CD224018A" modified="2014-01-22" version="1">MDTYSKIARAYDEQVRGELAHKPLDRALLTAFLELAGHGTVADVGCGPGHVTRFLADRHPGVIGVDLSPGMIAIARERVPDSTFSVGSMLSLPAADSSWAGAVALYSIIHLTPAERAVAFGEFARVLKPGGPLLLSFHVDSAEVAAGESTHLTEWFGSEVDVDVHFLDPVVITRELTAAGLAVQATLIRKPIAGAEFPSRRAYLTACREPASEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W792</accession>
    <name>U5W792_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MerR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561214.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33095"/>
    <dbReference type="EnsemblBacteria" id="AGZ44877">
        <property type="protein sequence ID" value="AGZ44877"/>
        <property type="gene ID" value="AFR_33095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33095"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_8_1_11"/>
    <dbReference type="OrthoDB" id="1661714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="14548" checksum="FA682B6D9F317BAA" modified="2014-01-22" version="1">MQTYSPGEIADRTGFSLDTLRYYERIGLLDDIARTPGGQRIFNDGDVAWLEILRCLRDTGMPIAQMRRYADLTRDECTMRERVDLLEEHDREIGRRLEELAAQREHIRGKIRYYRSLDVPAEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7A2</accession>
    <name>U5W7A2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561380.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33925"/>
    <dbReference type="EnsemblBacteria" id="AGZ45043">
        <property type="protein sequence ID" value="AGZ45043"/>
        <property type="gene ID" value="AFR_33925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6867"/>
    <dbReference type="eggNOG" id="COG3542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088365_0_3_11"/>
    <dbReference type="OrthoDB" id="1565693at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06121">
        <property type="entry name" value="cupin_YML079wp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009327">
        <property type="entry name" value="Cupin_DUF985"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039935">
        <property type="entry name" value="YML079W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387">
        <property type="entry name" value="PTHR33387"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06172">
        <property type="entry name" value="Cupin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_5" evidence="1">
        <location>
            <begin position="8"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="14924" checksum="64CAB070A8C627BB" modified="2014-01-22" version="1">MTTRPPLAERLDLQPHPEGGWFRETFRSAVTFTPDGYAGPRAAATGIYFLLMPDQFSAWHVVTSDELWFLHQGSLELRFGGAGAGPEEGGETVLLTADNPQALVPAGVWQSAYPVGGEPVLVSCVVSPGFDFEDFRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7A9</accession>
    <name>U5W7A9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribokinase-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33855</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561366.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33855"/>
    <dbReference type="EnsemblBacteria" id="AGZ45029">
        <property type="protein sequence ID" value="AGZ45029"/>
        <property type="gene ID" value="AFR_33855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6853"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="3">
        <location>
            <begin position="27"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="28433" checksum="6B616F0EAD0BF0E3" modified="2014-01-22" version="1">MLLIVGEAIVAYQREDDGPFTGPWPSGSPAIAAYVAGRLGVPTTFVGGIGRDDHGRVMTDGLAAGGVETGHLAVSGDRPTATASITYRGEEREFDFRVTGSAAVQVTDRDLGDLPERAGWLHLSGSALIFGEPLAATTLTALRRARAAGARISVDPNVRPSALTPATRDALVEVLGLAHVLLPSEGELEALGLDPAGFGDAVVCTTYGSGGATVRDASGSVHLDALPVDAIDTDGAGDSFAAGFIAASLAGASPLDAAREGIRVAAEAVQVVGPMTVRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7E5</accession>
    <name>U5W7E5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560257.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43920">
        <property type="protein sequence ID" value="AGZ43920"/>
        <property type="gene ID" value="AFR_28295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5736"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_585021_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="406" mass="43094" checksum="ADCF63A039A81266" modified="2014-01-22" version="1">MAKLVSGKDEGNSGQFSVAWQLEYPGDANGKDTDTERLYASWLVGDVVVRAQSDGVVGYSISDGSQVWGTPVPSGSIMCGATLTQQDGFAAITYGSSKKCDSVAGIDLRTGKETFTAKIEAEDTKGRFDTKAPLLNIVGKYVVVSMEDKVTAYEVADGKRRWTRTGGLGCTTGAQMAAGNGIVLVSEKCLGKGDKLIAVDAGTGIPKWAATVPRGSYITDIFSASPALARISPPGLDPVLLSFDAKGTKLAEIPQKLDGLEMIDYNLTARIGDHYGQRRYAFHIVGTTLYAPTMSWLTSKPDNQIVAVDLTTGKLKWGSRGSTMQFHTIRADESGVLGFEDGRFEQSPRLVRLDPATGATTTLATGAKAESLDVPSSADAHETAGTILFLPLEQPVTKKVAILALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7R9</accession>
    <name>U5W7R9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arginase/agmatinase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_34590</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34590"/>
    <dbReference type="EnsemblBacteria" id="AGZ45174">
        <property type="protein sequence ID" value="AGZ45174"/>
        <property type="gene ID" value="AFR_34590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6997"/>
    <dbReference type="eggNOG" id="COG0010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="PTHR11358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="391" mass="42856" checksum="06CE606BA5917852" modified="2014-01-22" version="1">MGFNHQHDDQHEHVEQTETEPTLHDRYGPEAAYAVAAEAKLPTTKWQEEIDRGLEYGLQGADSIKDRTIPTFSRGELPHFAGINTFLKAPYVEDVRRCGEYDVAVLGAPFDGGTTYRSGTRFGPQGIRKISALYGPYSFELGVDLRESITIADLGDIFTIPANIEKTFDQISKAVGHVYESGAFPVVLGGDHSIGYPTVRGVAQHLQGNLGIIHFDRHVDTQETDLDERMHTTPWFHATDIPNVPPKNLVQIGIGGWQAPRPGVKVGRERGTTIMTVTDCVEMGIEQAAERALEVAWDGAEAVWLSFDVDCLDAAFVPGTGWPEPGGFLPREVLKFIQIIADARPLAGIEVVECAPAYDTAEITALLATRVICDTLGCLVRSGHLPQRTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W825</accession>
    <name>U5W825_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_35215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35215"/>
    <dbReference type="EnsemblBacteria" id="AGZ45299">
        <property type="protein sequence ID" value="AGZ45299"/>
        <property type="gene ID" value="AFR_35215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7125"/>
    <dbReference type="eggNOG" id="COG1716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080081_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="366" mass="38087" checksum="03397F2647FA9DA1" modified="2014-01-22" version="1">MSQPPNQPYPGQPYPEQPGSGPPWSGQPGNPPTQPYPSQPGYPYQGQPNPDVPPSQPGYGPPAQPSYPPSGSYGPPADQGQPSYPPPDQGQPSYGQPQYGQPDYGQPQYGQQPQYGQPQYGQPQYGQPDYAQPGYPQSGPPMQGYPPPPKKKSKALPITLVSIAVVLVLCVGGGTAIYLAGRNASDSIVDTLNSAAPTAPTLPTEKTTTEPPASTITVVEPRTLGGRPKLTDPQFASAAEQLKQGLTKVPNASETVGALYGTPAKQDIVIVAAAAADVSDPKRELDGTFLGAGVSGLKLTGISTADPGRLGGAAKCGRGEAGGAPLIMCGWADEGSVGWIIWYYKSMSQAKSELPSLRGQIEKKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8H9</accession>
    <name>U5W8H9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561849.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36285"/>
    <dbReference type="EnsemblBacteria" id="AGZ45513">
        <property type="protein sequence ID" value="AGZ45513"/>
        <property type="gene ID" value="AFR_36285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7343"/>
    <dbReference type="eggNOG" id="ENOG503161G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148679_0_0_11"/>
    <dbReference type="OrthoDB" id="1837954at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019592">
        <property type="entry name" value="DUF2469"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10611">
        <property type="entry name" value="DUF2469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="108" mass="12999" checksum="34558785F4AF1E26" modified="2014-01-22" version="1">MSAEDLEKYETEMELQLYREYRDIVRQFSYVVETERRFYLANQVDLHVRNSDGEVYFEVEMHDAWVWDMYRPARFVKNVRVMTFKDVNVEELEKPDISLPDEAGFGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8K4</accession>
    <name>U5W8K4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_36090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561810.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36090"/>
    <dbReference type="EnsemblBacteria" id="AGZ45474">
        <property type="protein sequence ID" value="AGZ45474"/>
        <property type="gene ID" value="AFR_36090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7304"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="3">
        <location>
            <begin position="328"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="451" mass="48303" checksum="7F5BDB554F1C80DF" modified="2014-01-22" version="1">MTVVSIDNWRRPGPTARQRRIDLGIGLGVAVLAVLNVFFARSVGLFATDSAPAVPEQICWTLAVTLPLIWRRQRPELVAVLAAVFFIGGQVRGAQEQQLTSGAVIAAIYTLGAWGQKRFRARVLRVLIIAAMFVWLGVAWFMSHGQVPDGGPPGASGELPPLLAAVMNSMIINVAVFGFAYFLGETVWQSARRQDQLEVQAAELRAAQARVGERAVFGERVRIARELHDVVAHHVSVMGIQASACRRVLDKDPERARTSLTAVEDGARTAVDELRRMLGALREDGGNTEEQSAGAGIDHIEEVVGRAREAGLTATVQVYGEPVPVRDSISQAAYRIVQEAVTNTLKHARATTLDVRIRYLAGELEVDVADDGHGTVIRQGGAARGDGLGLVGMQERVAVHDGTLENGPRTGGGYRIRARFPITRDPARTLAAAGEAAGEAAGEAAEEAGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>U5W912</accession>
    <name>U5W912_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Copper-translocating P-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_37120</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562014.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37120"/>
    <dbReference type="EnsemblBacteria" id="AGZ45678">
        <property type="protein sequence ID" value="AGZ45678"/>
        <property type="gene ID" value="AFR_37120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7510"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_11"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="660"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="683"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="712" mass="73104" checksum="CD0BAA08AE087C72" modified="2014-01-22" version="1">MTQQIELEIGGMTCAACANRIEKKLNRLEGVTATVNYATEKARATVPAGLSAADLIAVVEKTGYTAAEPKAEPVAEAQRADPLRTRLLVSAALSVPVIVLAMVPAWQFTYWQWLSLTLAAPVVVYGGWPFHRAAFVNLRHGAATMDTLVSIGTLAAFLWSLWALFLGDAGVPGMTHPFELRAGSQGDALYLEAAAGVTAFLLAGRYAEARAKRRAGDALRSLLALGPKTVTLADGREIPADKLQAGDTFLVRPGEKVATDGTVVDGASALDQSLLTGESVPVEVAAGDPVTGGTVNAGGRLVVRATRVGADTQLAQMARLVEEAQNGKAAVQRLADRISGVFVPVVIALAAATLGYWLGTGQGTTAAFTAAVAVLIIACPCALGLATPTALLVGTGRGAQLGILIRGVEALESTRRVDTIVLDKTGTVTTGTMTVSSIVPEPGQDADELLRLAGAVEAASEHPLGRAITRAAAAKGSLPPVSDFRATAGVGVRGVVDGAEVEIARGTGDTAETWVEVRVDGTARGRLALSDSVRPTSAAAVRSLRALGLEPILVTGDAPAVAEAVAAQVGITEVIAGVLPAGKVDVVKQLQARGRSVAMVGDGVNDAAALAQADLGVAMGAGTDVAIEASDLTLMRDDLTAAVDAVRLARRTVTIIRGNLFWAFAYNVAGLPLAAAGLLNPMIAGAAMAFSSIFVVANSLRLRRFKAAAPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W929</accession>
    <name>U5W929_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Trans-aconitate 2-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560834.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31200"/>
    <dbReference type="EnsemblBacteria" id="AGZ44500">
        <property type="protein sequence ID" value="AGZ44500"/>
        <property type="gene ID" value="AFR_31200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6315"/>
    <dbReference type="eggNOG" id="COG4106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_5_2_11"/>
    <dbReference type="OrthoDB" id="1739932at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030798">
        <property type="term" value="F:trans-aconitate 2-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023149">
        <property type="entry name" value="Trans_acon_MeTrfase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="33"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="28398" checksum="BDB352658248EC4E" modified="2014-01-22" version="1">MWDPAVYHRFGAERSRPFFDLTNRIGATEPRAVTDLGCGPGELTASLAERWPGARVTGVDSSPEMITKARDLGAPVEFTVADLRDWEPGPGTDVVVTNAALQWVPGHQQLISRWVRALPPGAWFAMQVPGNFGAPSHRALREVARSSAYAETVGELVREAPVDDPVGYAGLLADAGAAVDAWETTYVHLLPVAGEDHPVLRWMEGTALRPVRAALDDTRWQDFRAALGARLAAEYPARNGFVAFPFRRIFVVATTAQGERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W957</accession>
    <name>U5W957_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_37050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562000.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37050"/>
    <dbReference type="EnsemblBacteria" id="AGZ45664">
        <property type="protein sequence ID" value="AGZ45664"/>
        <property type="gene ID" value="AFR_37050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7496"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_3_11"/>
    <dbReference type="OrthoDB" id="391134at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="9"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="3">
        <location>
            <begin position="192"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="378"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="540" mass="55967" checksum="6BB81C5B0F4DDA5C" modified="2014-01-22" version="1">MAEMIEGHGGDLALAALRAYGVTEMFTLSGGHVFPLYDAAHKAGFPIYDVRHEQSAVFAAEAVAKLQRRPGLAVLTAGPGVTNGISGLTSAFFNASPVLVMGGRAPAFRWGSGSLQEIDHVPLVKPVTKYAATVGGTDEIPAEVAKALTAALTAHRGPAFLDLPLEVLFSSEDAAAPGAPEIPVIEPDPDDVARAAALLAGAERPVIIAGSDVWGGNAIAELRAAAEALQVPVFTNGMGRGALPPGHPLSFAKARRPALNEADVVAVIGTPLDFRLGFGEFGAAQVVHIVDAPSQRAGHVETAVSPAGDLRLILTALANHTGSRADHTEWVEGLRTAEDAGKARDAEAMAAESDPIKPARIYGELRKVLAPDAVTIGDGGDFVSYAGRFLEPAQPGTWLDPGPYGCLGTGLGYAMGARVTYPDRQICVLMGDGAAGFSLMDAESLARQGLPVVIVVGNNGIWGLEKHPMQAMYGYDVAADLQPGLRYDRVVEALGGAGETVEKSSDLGPALTRAFDAGVPYLVNVLTDPADAYPRSSNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9C7</accession>
    <name>U5W9C7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTTM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_37795</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37795"/>
    <dbReference type="EnsemblBacteria" id="AGZ45813">
        <property type="protein sequence ID" value="AGZ45813"/>
        <property type="gene ID" value="AFR_37795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7643"/>
    <dbReference type="eggNOG" id="ENOG502ZC87">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072042_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011020">
        <property type="entry name" value="HTTM"/>
    </dbReference>
    <dbReference type="SMART" id="SM00752">
        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="HTTM" evidence="6">
        <location>
            <begin position="5"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="31765" checksum="BB477167626FFE2B" modified="2014-01-22" version="1">MKWLFAPVPLGRVAAFRTLIYLFVAADLVIFTPWVRHHASTPGDLYEPLLVGRLLHLPTPTPLLVHGIFWALLVLALAAATGRAPRLLGWTVFALYFEWMVIAMSYGKVDHDRVGLLVALAVLPTVGRARHGDPTRTESGGWALRVTQVAVVCTYFLAAWAKLRFGGLDWVTSSVLARAIVRRGTDLADLIAGVPYLLILAQVGIIAFELASPAIFFLKPRWRNYAVAFFYSFHVVTFATITISFAPHLAAITSFLALEKVRPVVWVRGFLARRRGEVESHERPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9D9</accession>
    <name>U5W9D9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetolactate synthase small subunit</fullName>
            <shortName evidence="8">AHAS</shortName>
            <shortName evidence="8">ALS</shortName>
            <ecNumber evidence="8">2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetohydroxy-acid synthase small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ilvH</name>
        <name evidence="10" type="ORF">AFR_36890</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561968.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36890"/>
    <dbReference type="EnsemblBacteria" id="AGZ45632">
        <property type="protein sequence ID" value="AGZ45632"/>
        <property type="gene ID" value="AFR_36890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7463"/>
    <dbReference type="eggNOG" id="COG0440">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055003_1_3_11"/>
    <dbReference type="OrthoDB" id="1500398at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="PTHR30239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="18634" checksum="2AA182237C5003AF" modified="2014-01-22" version="1">MNKHTLSVLVENKPGVLARVSGLFSRRSFNIDSLAVGETENPDVSRITIVVNADSSPLEQVTKQLNKLVNVLKIVELDPQQSVQRELLLVKVRADRTVRGQVLETVELFRAKVIDVAPDTLTIEATGNPDKLDALLRDLEPYGIKEMVQSGLVAIGRGSRSITTGPALRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9L7</accession>
    <name>U5W9L7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_37925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562174.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37925"/>
    <dbReference type="EnsemblBacteria" id="AGZ45839">
        <property type="protein sequence ID" value="AGZ45839"/>
        <property type="gene ID" value="AFR_37925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7670"/>
    <dbReference type="eggNOG" id="ENOG502ZHFH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2754022_0_0_11"/>
    <dbReference type="OrthoDB" id="1890863at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="7858" checksum="9A6F0F1A3B53CA90" modified="2014-01-22" version="1">MLCWFCAKAARGSCRFCGRGVCEDHARFGPYLLQVSRSEVRGRAEALVVEDAVQCGTCRPRPQPVAMPELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9N0</accession>
    <name>U5W9N0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF305 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32110"/>
    <dbReference type="EnsemblBacteria" id="AGZ44680">
        <property type="protein sequence ID" value="AGZ44680"/>
        <property type="gene ID" value="AFR_32110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6501"/>
    <dbReference type="eggNOG" id="COG3544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074343_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005183">
        <property type="entry name" value="DUF305"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03713">
        <property type="entry name" value="DUF305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665837" description="DUF305 domain-containing protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="DUF305" evidence="2">
        <location>
            <begin position="66"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="19496" checksum="84BBC2AA26F02111" modified="2014-01-22" version="1">MIYPQRTAVAGCLLALLLGASACDAGTASAPPPAASSAASSFFGGTDLAWVEINIAMNEELLPLLDLVPAHSTDPEVKALVTEVRAVNDQELATLRALHDEAKLPAENPHKGMPMPGMMTPELVAEAAKVRGPAFDKLLLAHLEAHFDQGVRLAGSEEKSGLEPRTKALAGEVIGTRKKYLPKVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9X5</accession>
    <name>U5W9X5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561053.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32315"/>
    <dbReference type="EnsemblBacteria" id="AGZ44721">
        <property type="protein sequence ID" value="AGZ44721"/>
        <property type="gene ID" value="AFR_32315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6541"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025711_4_5_11"/>
    <dbReference type="OrthoDB" id="948454at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="207" mass="21706" checksum="F2DE439405133795" modified="2014-01-22" version="1">MRITVCGSTGLTGAEIVRRAIEGGHAVTAVARSEFTGFPAGVELVRADVMDPSAIEPAVAGSDIVISALGHRPGHDEPVCAPGAESIITAMRATGVRRLVVITASGHVTDSHDDFLTARVVKPLLKRFLRASFTDFEATESLVRASGLDWTIMRPPRLTNGSSKAYRTAVDHTVRRGNLIARTDLAAATLDAAANPATIGHAVAIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA15</accession>
    <name>U5WA15_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative anti-sigma regulatory factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38040"/>
    <dbReference type="EnsemblBacteria" id="AGZ45862">
        <property type="protein sequence ID" value="AGZ45862"/>
        <property type="gene ID" value="AFR_38040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7695"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116681_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="14608" checksum="347B82D5E16CE21A" modified="2014-01-22" version="1">MTQLQTTHPEPAFGDDVVLLTVPADGGYLGVLRTATAGLAARLHFALDEIEDLRIAVDEACAMLLAIATRGAELECRFAVTDDALTVEVTVSTVRGARLPSESSFAWKVLTALTTSASAEANGRHATIRLLTRRTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA71</accession>
    <name>U5WA71_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561173.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32890"/>
    <dbReference type="EnsemblBacteria" id="AGZ44836">
        <property type="protein sequence ID" value="AGZ44836"/>
        <property type="gene ID" value="AFR_32890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6656"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_5_6_11"/>
    <dbReference type="OrthoDB" id="1442962at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="20927" checksum="CBE0CD6FFAF135DF" modified="2014-01-22" version="1">MTNALIVIDVQESFRARPLWQAVSNPGIVAQVQRLVDAARAAGDPVVWVLHAEPGTGGVFDPASGFVRVVDELVPAAGEPIITKTSHNAFTTTNLQQLLTAGGVRELTVCGIRTEQCVETTARVGSDLGYAVTFVTDATATFPIPHRDAPEDLTVAELLADPRTLAAEDVVTRTEYALTGRFATVRTVAELTGSLTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAX0</accession>
    <name>U5WAX0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562476.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39440"/>
    <dbReference type="EnsemblBacteria" id="AGZ46142">
        <property type="protein sequence ID" value="AGZ46142"/>
        <property type="gene ID" value="AFR_39440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7979"/>
    <dbReference type="eggNOG" id="ENOG5032S7P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061970_2_0_11"/>
    <dbReference type="OrthoDB" id="2033191at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="33683" checksum="15CE80A8D065D9E5" modified="2014-01-22" version="1">MPESTEELIRALYEAPPDGFVAGRAAVVAEAKKAGDKDTAKRLAALRKPTVAAWLVNLLALRRPELIDELVELSTALRSAQRGLHGDQLRELSTQRRQVVSALVNAAQKLAVEDDPGLRSVKLPMAEVEATLTAALAEPEIAEQVRSGRLVKAVSYAGFGEVPRPRLRLVTAEDTPDEEADEEKEEERPSALDGRKARAAEKARQERAEKRRELKRELTAARAADTRARARLERAEEAERDAAHAVDEIEEQLAELERQRIVAQAEVSRRKLARRSAERDASAARRQVGDVEAAIEDIDADEPDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAY8</accession>
    <name>U5WAY8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative LuxR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34165"/>
    <dbReference type="EnsemblBacteria" id="AGZ45091">
        <property type="protein sequence ID" value="AGZ45091"/>
        <property type="gene ID" value="AFR_34165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6913"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3899">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="838"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="902" mass="97944" checksum="959D8DA6DAAFDE6B" modified="2014-01-22" version="1">MMIGRESELRRLARLAAAREPAVALIAGEPGIGKTRLVHELMAVLPPETVVLVGQAEPGSLSRPYEVLLDAIDGRPEVDQEQLAALADPRRSPVERLHTGLAVLAGLIGDSPAVVVFEDLHWADSESAALFERIADQRGPRLLVGTYRPDEVTRRQPVAGLLARMERRHTLTHVRLDRLSPTQTAAFLAAATGAPAPLRAATALHHRTGGNPFFLEELLRALPGYDVEALVDQPLPWSLADVLRRQVDDLEPLSHRIVEAATVLGHRIPFDLLADVTGTGEDELIAVLRDLVTRGVLVESGEDEFAFRHALVREAIGDQMLGRQRRRLHETALDVLLRGGASDPAMVAHHARGAGRYDDMVTAARRGAALYLSIGSAYQALQLAEMGLDEASDDTELLAAGARAAWLAGLLDDALRYGRRWRDLATTTTDRAESLYLLVRLAWESRETGAMRALTHDIETLIAQLPPGADQARAMTAIAQSAYLRDDLDGALLWSDRALALADEFDLPAIRLAALLEKGSTLTERPQTATAGREILSALVDEAEKAGEWVLAARALDILVQAVPPASPADYAESLERMRVDAERAGFESLAVATYFQGRARLAMRAGDLRAAIAALEEGREQDRSYRRRGRWADYHGVFLAGLYLEAGELDRVTQLIADLETVPNNPPTTIPGLAFHLACRRSDLPRAESTLDDLLEALTAQNWRSGEQAHNLVAAALELGLPADRLDHMAAALLDAEVWDAFRTLVGAQLAEAHGRHAEALTAYESVLEAHILHPAYRGTVRVAAARCLLADDRRGEATAYVEAAVPLLAGWRGWRVAQLDQVRARLGMAPADAPPAITGPAALTPREREVAVLISDGLTNTELARRLYISPKTAAVHVSNILRKLGASSRTEVSDLIGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBB2</accession>
    <name>U5WBB2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562609.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40115"/>
    <dbReference type="EnsemblBacteria" id="AGZ46277">
        <property type="protein sequence ID" value="AGZ46277"/>
        <property type="gene ID" value="AFR_40115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8118"/>
    <dbReference type="eggNOG" id="COG3599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099415_1_0_11"/>
    <dbReference type="OrthoDB" id="2060110at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019933">
        <property type="entry name" value="DivIVA_domain"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03544">
        <property type="entry name" value="DivI1A_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="180" mass="18850" checksum="45CF5B7C09B4F58A" modified="2014-01-22" version="1">MGQLFLFIVVGLVVAAIVFGVTVLVSGGDSGLTPVEPDGRSLPLPSDRPLGEEDIFRTRFDTGFRGYRMAQVDQALQRAAYDIGYKGELIGVLEAEVAALREGRTADADVLRRAREAAVAPPPPAPAEDAQPEGSLPEAPPPPGKPVTEAEPAATAVKDDPTPEEATARAAGDGEPAERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBH7</accession>
    <name>U5WBH7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flagellar hook-length control protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562679.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40465"/>
    <dbReference type="EnsemblBacteria" id="AGZ46347">
        <property type="protein sequence ID" value="AGZ46347"/>
        <property type="gene ID" value="AFR_40465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8190"/>
    <dbReference type="eggNOG" id="COG3144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_417188_0_0_11"/>
    <dbReference type="OrthoDB" id="1966347at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021136">
        <property type="entry name" value="Flagellar_hook_control-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038610">
        <property type="entry name" value="FliK-like_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02120">
        <property type="entry name" value="Flg_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_hook" evidence="2">
        <location>
            <begin position="533"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="657" mass="65609" checksum="DBBF6CEAD40A21A2" modified="2014-01-22" version="1">MTSSVTGPDRKPAVDTARRGPGKPGEGAEAFGSALSAELDRKPADQADRKAQDRAVAGRAAQDRLQAENRAADARLQRSAATRNQIAAQQRLSQARAAAQRTTAQRPDAPERPDAPQRPDAPQRADSPQRPEASKRPEPSQRSEASERPDASERPSEDPGSPRETGAPETRRPETSRSDKPSTGAEVKQAETTVSSEPAAEADSATATDEPTTPEKAEATAVPVVALPFQAALAATTEVPDGEAPPAATTSVTAPAPPTTPAPPAATPEAPAGPITNAGASQAAAWAQAEAQAAARTGEAGMSLAPSSAAQTAVTQADAVSQAAVMQAAPQATAAPQNAAAPAAPQAAAPAATSAMPPDIAEAVADGLGAPAATATAAAPTAAAAPTTAAAPTAQATPAAPAAAPTADVAATAAADLTAVPTTGQTETTAEPAPTADQPPTTTSGTEGETGTGGQQTDQQSSNGQPGTFVAPATAAATPGTEFPGGVPQVGGVAATAPTAPAAPVADTAPPAPPTPGPVAAQVATRITPLRLDADGVHRLTINLHPVDLGPVQVVAEIRNGEINVQLSSSTDAGHDSLRNAMDDLRRELEQSGFSNTSLDLRQGSQQEQARQQFAFMGENNGTRGGNRGRGAEPEPEAPAPPSPSRTDGNSRLDIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBT0</accession>
    <name>U5WBT0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DeoR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_41015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562789.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41015"/>
    <dbReference type="EnsemblBacteria" id="AGZ46457">
        <property type="protein sequence ID" value="AGZ46457"/>
        <property type="gene ID" value="AFR_41015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8302"/>
    <dbReference type="eggNOG" id="COG1349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060699_0_1_11"/>
    <dbReference type="OrthoDB" id="1629121at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014036">
        <property type="entry name" value="DeoR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018356">
        <property type="entry name" value="Tscrpt_reg_HTH_DeoR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00455">
        <property type="entry name" value="DeoRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08220">
        <property type="entry name" value="HTH_DeoR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00037">
        <property type="entry name" value="HTHLACR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00420">
        <property type="entry name" value="HTH_DEOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00894">
        <property type="entry name" value="HTH_DEOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="27459" checksum="174299FDF2B4B2DD" modified="2014-01-22" version="1">MDRYARWNALLELLAESGRVTVEDAADKLDVSQATIRRDFDQLAQQQMITRTRGGAVANGVSYDLPLRYKSAKHSAEKQRIGEAAANLVVPGTVVGLNGGTTMTEVARALAVRPDLNTSGEGAQLTVVTNALNIANELLVRSRMKIVVAGGVVRPQSFELVGPLGGALLKEVTLDIVLLGVDALDVELGAAAHHEGEAAMNSLMVARAKRVVVIADSSKLGGHAFARICPISKVETLVTDSGAPAELLEGFRDAGVQVVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBY4</accession>
    <name>U5WBY4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562960.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41875"/>
    <dbReference type="EnsemblBacteria" id="AGZ46629">
        <property type="protein sequence ID" value="AGZ46629"/>
        <property type="gene ID" value="AFR_41875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8473"/>
    <dbReference type="HOGENOM" id="CLU_2217343_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="106" mass="11557" checksum="D7364698435BB2ED" modified="2014-01-22" version="1">MSPEDLRGWDHYPAKIRSLRITIGAFLLASATLALMSLDVQVQLLGVLAATLIIVFCIALLSALPLFARVSRINATEAHARGGHQINDSLPLLRTIVTDVLRGRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCF8</accession>
    <name>U5WCF8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative HTH-type transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561937.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36725"/>
    <dbReference type="EnsemblBacteria" id="AGZ45601">
        <property type="protein sequence ID" value="AGZ45601"/>
        <property type="gene ID" value="AFR_36725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7429"/>
    <dbReference type="eggNOG" id="COG1476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_44_1_11"/>
    <dbReference type="OrthoDB" id="2063007at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="82" mass="9211" checksum="6E4E808B74ADFAB9" modified="2014-01-22" version="1">MSSEITYNRIAMLRAERGISRRQLADALGVHYQTVGYLERGEFSPSLHLALRICDYFEVPVEVVFSLSPFPRLGAEPAPRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCG8</accession>
    <name>U5WCG8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">EmrB/QacA subfamily drug resistance transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_42040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562993.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42040"/>
    <dbReference type="EnsemblBacteria" id="AGZ46662">
        <property type="protein sequence ID" value="AGZ46662"/>
        <property type="gene ID" value="AFR_42040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8507"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_11"/>
    <dbReference type="OrthoDB" id="1108236at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="22"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="489" mass="49673" checksum="59DA8F4E75F87A60" modified="2014-01-22" version="1">MYAAQGGACVEVEPVQRNPWSTLAVLALAQFMVVLDVTIVNVALPDIQQDLGFSASGLQWVVSAYTLLFGGFLLLGGRAADLLGRRRLFIIGLLVFGVTSLIAGLAQNPEQLVIMRAVQGLGGALLSPAAFAILTVTYAKGRERNIAMGVWGGLAGLGGTLGVIAGGLLVDSLSWRWVFLVNVPIAVALAVIVPLFVRESKITSTGTRTFDVAGALLSTAGLLAIVLGVIRAEPEGWASLQVIALLVGGLAMLGAFIGVEARSSAPLVPLSLFRSRGLTTSILALALNGAAFLAMFFLTAIFLQQVRGLTALETGLQFLPMGIAAITSAVVSAQLVTRFGTKPVQFGGAALSVVGLLLLSGADATGSYALTILPGLILFGMGIIATGTPASIAAVSDVEHEQAGAASGVVNAGYQVGGALGLAAITTFATSHVEGLLTGGSAPQDALVGGFERGLLIAAIFAAANIAIALGAPRLRPTIDEVENAAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD39</accession>
    <name>U5WD39_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-like N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38035"/>
    <dbReference type="EnsemblBacteria" id="AGZ45861">
        <property type="protein sequence ID" value="AGZ45861"/>
        <property type="gene ID" value="AFR_38035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7694"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_41_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="18350" checksum="BADE3D3851D5CA0D" modified="2014-01-22" version="1">MSPATIRPAHRDDVPAVVAMVHELADFERAAESCHLTEEQLTAALFGPQPALFGHVAVDAQDSPYAFALWFLNFSTWEGTHGIHLEDLYVRPQARGTGAGSALLATLAAECVRRGYQRLEWVMLDWNPAAEFYAAIGAKVTEDWLPYRLTGSALRQLAERSSPARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFE0</accession>
    <name>U5WFE0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative cell envelope-related transcriptional attenuator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_42505</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42505"/>
    <dbReference type="EnsemblBacteria" id="AGZ46755">
        <property type="protein sequence ID" value="AGZ46755"/>
        <property type="gene ID" value="AFR_42505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8602"/>
    <dbReference type="eggNOG" id="COG1316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016455_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00350">
        <property type="entry name" value="lytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="LytR_cpsA_psr" evidence="4">
        <location>
            <begin position="150"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="403" mass="42836" checksum="8F3535D5CF023F93" modified="2014-01-22" version="1">MSRASVPGPGRVGGGRPSSGRAGVPGGYNDRPAGRDDWPPRGSGGGPAGPPRGPSGTRPGGRPYKGKRKPKWGRIALVVAAVLALGGVIAGISLYGYANGLNDDLKRTDAFSDITNGRPAKTVDGALNILLVGSDSRDPDATKEDTNQWRADTLIVMHIPADHKSAQLISIPRDLWVQIPSGNSADCSSGSRAKINAAFAFGGLPRAVRTVECMTDVHLDHVLAIDFGGFKEVTDALGGVDLKVEQNITSIHKPFRKFKKGTMHMNGAQALDWIRQRKQFARGDFARMQHQQEFLKALMDKAASSGTLSNPAKLNSFLKATTNAITADNDFSLTDMAVQFRSLRGENLTFLTSPNLGSDTIAGQSVVVSDRENALAMYKAVAADKMDEWMAQNKTAPSSQAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFU2</accession>
    <name>U5WFU2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42760"/>
    <dbReference type="EnsemblBacteria" id="AGZ46806">
        <property type="protein sequence ID" value="AGZ46806"/>
        <property type="gene ID" value="AFR_42760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8654"/>
    <dbReference type="eggNOG" id="ENOG5033U9G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_670514_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025850">
        <property type="entry name" value="SUKH-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025968">
        <property type="entry name" value="YwqJ_deaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14433">
        <property type="entry name" value="SUKH-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14431">
        <property type="entry name" value="YwqJ-deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="410" mass="44760" checksum="BCE0BE421CB977E7" modified="2014-01-22" version="1">MITREDAEEIAASWARGESEVRGYECQPMISEFDLGFVVWTKQPPAVLPIPGDGARTVIDRETGALSTYPGVPAEVIAEMYRTHQPSVAARRRTVDPQVELRRNAKRRASPTTAAHLTVDGHLFIARGAKGDQEINHHPLVAAYLSSLEPARTVRGTDRHAELIVLSDVLHEADRQRDVAITLDEARAWLRTADFESFFVRDQGDPLGGTSARPCESCILTLVDFAVLPWSQLAFTEPFVPYSDNIAQPGRFPDHVAGALADAGWRPMQQVVAEVLADGMIEDVVNVPGKRFQHRVFPAAREAVMAFPGIFCGLRGPGVRRWVRWLNLEPAAAAHSADALGELAAVIGAPLFPIGVEAKGDTIVAIDERGRIFALDQGGEWFLGNTLDEGLLSLLTGDGPAERLRDDGTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNT6</accession>
    <name>U5VNT6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38462">
        <property type="protein sequence ID" value="AGZ38462"/>
        <property type="gene ID" value="AFR_00865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.173"/>
    <dbReference type="eggNOG" id="ENOG5031VMQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1965812_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="127" mass="14215" checksum="E9AAF165D6D07B72" modified="2014-01-22" version="1">MNGDNGEQMTISVVRVEDPELSSFPELKPATGDRLVSVTVNAKNTGTVPFIAQLQKYAWVADAKDEWYQRDTTMTAALAAFPDALIEPRWEFARTIVFEMPQDAELTRLRLSLKPGVAAQTPEWMLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP91</accession>
    <name>U5VP91_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chorismate dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.151</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Menaquinone biosynthetic enzyme MqnA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mqnA</name>
        <name evidence="4" type="ORF">AFR_01575</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the dehydration of chorismate into 3-[(1-carboxyvinyl)oxy]benzoate, a step in the biosynthesis of menaquinone (MK, vitamin K2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>chorismate = 3-[(1-carboxyvinyl)-oxy]benzoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:40051"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:76981"/>
            <dbReference type="EC" id="4.2.1.151"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MqnA/MqnD family. MqnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.151" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357547.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01575"/>
    <dbReference type="EnsemblBacteria" id="AGZ38604">
        <property type="protein sequence ID" value="AGZ38604"/>
        <property type="gene ID" value="AFR_01575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.316"/>
    <dbReference type="eggNOG" id="COG1427">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059898_0_0_11"/>
    <dbReference type="OrthoDB" id="1062841at2"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00995">
        <property type="entry name" value="MqnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003773">
        <property type="entry name" value="Menaquinone_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030868">
        <property type="entry name" value="MqnA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37690">
        <property type="entry name" value="PTHR37690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02621">
        <property type="entry name" value="VitK2_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="283" mass="30769" checksum="DD373F1CEA1960D4" modified="2014-01-22" version="1">MSDAVQRPRVGHIQFLNCLPIYWGLMRSGALLDVDLHKDTPDRLNAALVAGDLDIGPISLVEYLRNADDLLLLPNLAVGSDGPVLSVNLVSTRPLDELDGRPVALGSTSRTGVLLAQMLLADKYGAEPEYFRCPPELTQMLLEADAAVLIGDPALRALYEAPALGLHVTDLAEAWKEWTGLPMVFAVWAVRKDFAAANPGVVKDVHEAFQRSRDLCLGELDDVAESAARWEPFDAATLATYFRALDFSLGERQIEGVREFARRAAARGEAPALPEDGPIFAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPA8</accession>
    <name>U5VPA8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA polymerase, sigma-24 subunit, ecf subfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_01740</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01740"/>
    <dbReference type="EnsemblBacteria" id="AGZ38637">
        <property type="protein sequence ID" value="AGZ38637"/>
        <property type="gene ID" value="AFR_01740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.351"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="15"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="7">
        <location>
            <begin position="113"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="19076" checksum="C469C9A3D4233237" modified="2014-01-22" version="1">MEDQDAELLRALQDEHGEALFAHAVRLSGGDRQRAEDLVQETLLRAWRYPEALDPERGAVRSWLFTTARNLAIDAWRRRSVRVGEVVTDMIPEPPPSADEADRAVEAWTVAEALGRLSTSHREVLVECFYQGRSVAEAAARLGVPPGTVKSRTHYALRSLRMVLDEMGVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPT7</accession>
    <name>U5VPT7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357916.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03420"/>
    <dbReference type="EnsemblBacteria" id="AGZ38973">
        <property type="protein sequence ID" value="AGZ38973"/>
        <property type="gene ID" value="AFR_03420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.688"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051674_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="27981" checksum="6EF24858BAEE3337" modified="2014-01-22" version="1">MSTATLSPPPDTAQAAAPVVASKLKVTQARVVRSEWIKFWSLRSTTVTLAAAVVLLVGIGLLASSMLNSGDTGGPGPDVLGPVSASLAGLTFAQLAFGTLGVLFMASEYSTGMIRSSLTAVPKRLPVLWGKIAVFAAVVFVVGLVASAVAFTGGQAIIGDGGASWSDPGVTRAVVGSAVVLAGSGVLGLALGALLRSTPAAISTLFGAMFLLSGVAQLLLPDAWRDDVVRFLPANAGDAFTAVTEGSGALTPWAGLAVFVAYVAAVVAGGAWRLKRSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQA8</accession>
    <name>U5VQA8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357937.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03525"/>
    <dbReference type="EnsemblBacteria" id="AGZ38994">
        <property type="protein sequence ID" value="AGZ38994"/>
        <property type="gene ID" value="AFR_03525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.708"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_4_11"/>
    <dbReference type="OrthoDB" id="1027479at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="10"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="322" mass="33717" checksum="08CE5679B518CFB0" modified="2014-01-22" version="1">MEAIVLDAPGPPEALQYRKLPVPVPQAGQVLIEVKAFGLNRSELHTRLGLGDGVTFPRVPGIEATGVVAAAPGGEFAVGQKVFAMMGGMGRTFDGGYAQYTCVPARQVIAFRSELDWATLGAVPEMLQTAYGALTEGLDAQPGQSLLVRGGTSSVGMAIAILAKRRGLTVLSTTRSPAKSDALRAIGVDHVLIDDGDVAAQVRAILPEGVDNALELVGTPTLPDTLRATRKLGVVCMAGMLSNVWTVPDFYPIGYIPRGVRLTGYGGDADDLPPAVLQDFLDAVAAGEAIVPLDRTYRFDQIVQAHADMEAGVAVGKFVVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRB0</accession>
    <name>U5VRB0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine triad (HIT) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358920.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05500"/>
    <dbReference type="EnsemblBacteria" id="AGZ39389">
        <property type="protein sequence ID" value="AGZ39389"/>
        <property type="gene ID" value="AFR_05500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1116"/>
    <dbReference type="eggNOG" id="COG0537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_3_3_11"/>
    <dbReference type="OrthoDB" id="1934382at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="PTHR46648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="5"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="95"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="14822" checksum="E9152E75CF81E576" modified="2014-01-22" version="1">MPDCLFCSIVAGTVPAFKVVDSPDGVGFLDIRPVFKGHVLVVPRPHLTQLSDLPADLLPGYFTLVQRVAAAVPRALGSQGTFVAENNVVSQSVPHLHTHVVPRTKGDGLRGFFWPRTRYSSDDEARDFSGRIAAELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRF6</accession>
    <name>U5VRF6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00105"/>
    <dbReference type="EnsemblBacteria" id="AGZ38310">
        <property type="protein sequence ID" value="AGZ38310"/>
        <property type="gene ID" value="AFR_00105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.22"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="66"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="12974" checksum="AD43F79AE04E0644" modified="2014-01-22" version="1">MTRRVVYLSGDTMLDEAAQAMRDQAIGDIVITNGPTLAGLVTDRDIVVRALAEGLPPRTTTLASIASRELIMIEQSATVEEAVQAMRDREVRRLLVCDADRNLVGILSLSDVALVPTSAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRG9</accession>
    <name>U5VRG9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Cell division protein FtsX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_05850</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter FtsEX involved in cellular division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-4 integral membrane protein family. FtsX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358990.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05850"/>
    <dbReference type="EnsemblBacteria" id="AGZ39459">
        <property type="protein sequence ID" value="AGZ39459"/>
        <property type="gene ID" value="AFR_05850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1188"/>
    <dbReference type="eggNOG" id="COG2177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073546_1_0_11"/>
    <dbReference type="OrthoDB" id="1653234at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004513">
        <property type="entry name" value="ABC_transpt_FtsX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040690">
        <property type="entry name" value="FtsX_ECD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47755">
        <property type="entry name" value="PTHR47755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18075">
        <property type="entry name" value="FtsX_ECD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003097">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX_ECD" evidence="10">
        <location>
            <begin position="56"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR003097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ39459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="32224" checksum="8A88AECAF0AD3049" modified="2014-01-22" version="1">MRFKYVLNEVLVGLWRNVTMTIAMIITMSVSLTMLGASVLMYMQVDQMKSYYYGEIEVSIFLREDVTEAQRAAITQAIDTNGLVQSKTYETRAQAFEKFKVLWRDSPDFVKSVGPDSLPESFRVKLKDPEQYQAFAEQIQGLPGIQDIVDQRELLDKVFKIFNAIQVGALVVAGFMALAALLLVGNTIQVAAYSKRREVAVMKLVGASNWFIQAPFVLEAVVAGLIGAILGFVFIFIGKVVLLDGRLQALTNVLTPIPDGNVWLMLPLLAGVGAGVSAITAWITLRFYLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRP9</accession>
    <name>U5VRP9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359023.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06015"/>
    <dbReference type="EnsemblBacteria" id="AGZ39492">
        <property type="protein sequence ID" value="AGZ39492"/>
        <property type="gene ID" value="AFR_06015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1222"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873269_0_0_11"/>
    <dbReference type="OrthoDB" id="2135016at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="SSF50969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="34728" checksum="8E211779159F7C25" modified="2014-01-22" version="1">MRPRLIAETTVPGLRWVEPIAGAGWVVTQPETPALTVLDTELRTLATVGLPAGDRDMQAAADDTLLAVGTGQELIALERDGQIRWRRSFGAGRPDPHFDDHGVLWVHLPPADELLAVEAATGREIDRVPLDSTHGGALFTRHAGGEWTGLHVAMGQDGSQSWLVRLDGGKIVLRELAGECVTGFLRAGELYFSTPHNDEQLSIREVATDAVVAEHAIEDVPGLPELGEDLILLEAATVVSDDFVLVAVNTDDFDTDLEDHLLLSTSTLGYRATMLYPYEMTHNSIYPADAPGRWLTVDYGENVLRLWQLEDEPEPGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRX0</accession>
    <name>U5VRX0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359254.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07170"/>
    <dbReference type="EnsemblBacteria" id="AGZ39723">
        <property type="protein sequence ID" value="AGZ39723"/>
        <property type="gene ID" value="AFR_07170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1463"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077925_0_0_11"/>
    <dbReference type="OrthoDB" id="1457558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="32970" checksum="DCC50E91D8A7459D" modified="2014-01-22" version="1">MVQTYGARIGWVDLPRHIRLQAERIAGGQVTAAVSQNGGFSPGTADRVVLGDGRRAFVKAVSTSHNERSVELARNEAHVTARMPGHAPVPRLLGSYDDGEWIVLVLEDVEGRHPRTPWVGEEVAATAGALRDLASAVTPPPLTGLPDAADHLAPHFAGWDLIAADPPPDLDLWVRAHLDDLRRAADRGLAAVRHGETLAHCDIRADNLLVRPDGTVRVVDWAWASIGPDWLDTVLLGMNVLVHGGDDRVLAGVDPRAAADVIAGFTGYLRERSRHPSPGIPHVRDFQRAQADAVLPWLRERLVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSC8</accession>
    <name>U5VSC8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MarR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07140"/>
    <dbReference type="EnsemblBacteria" id="AGZ39717">
        <property type="protein sequence ID" value="AGZ39717"/>
        <property type="gene ID" value="AFR_07140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1457"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_15_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17117" checksum="2011A15DDCDF58BC" modified="2014-01-22" version="1">MIKPEPGDAAGRLAEEIVRFTRLGARAKGMLNAGDLGAEFSALMLLFPLRHLGPMRVTDLAELKGTDPSTISRQAAQLVKAGLARREADPADGRASRLAVTDAGLAACGQLHEARHALLSEALSGWPAERVTAFADLFEEFNSSVEARLRSDPAVSARENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSE3</accession>
    <name>U5VSE3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359315.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07475"/>
    <dbReference type="EnsemblBacteria" id="AGZ39784">
        <property type="protein sequence ID" value="AGZ39784"/>
        <property type="gene ID" value="AFR_07475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1526"/>
    <dbReference type="HOGENOM" id="CLU_2581789_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="80" mass="8517" checksum="C308B4BC3B31C711" modified="2014-01-22" version="1">MAETDSVATSTIAVHRGVWDSFCPNLPADVAAMVAPPVTVSSLPVQSPAEPVAVERRARQAKMRGNMPSVPAYARAKRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSF6</accession>
    <name>U5VSF6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">clpX</name>
        <name evidence="9" type="ORF">AFR_07550</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ClpX-ClpP complex. Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the ClpX chaperone family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359330.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07550"/>
    <dbReference type="EnsemblBacteria" id="AGZ39799">
        <property type="protein sequence ID" value="AGZ39799"/>
        <property type="gene ID" value="AFR_07550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1541"/>
    <dbReference type="eggNOG" id="COG1219">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014218_8_2_11"/>
    <dbReference type="OrthoDB" id="718259at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00175">
        <property type="entry name" value="ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004487">
        <property type="entry name" value="Clp_protease_ATP-bd_su_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046425">
        <property type="entry name" value="ClpX_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010603">
        <property type="entry name" value="Znf_CppX_C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038366">
        <property type="entry name" value="Znf_CppX_C4_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06689">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00994">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00382">
        <property type="entry name" value="clpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51902">
        <property type="entry name" value="CLPX_ZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ClpX-type ZB" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="430" mass="47164" checksum="DE54C5E01C6DDF47" modified="2014-01-22" version="1">MARIGDGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNEIIEEELAETGEVKWEELPKPMEICQFLDNYVVGQEQAKKALSVAVYNHYKRIQAESAGAPGGGSDVEVAKSNIMLIGPTGCGKTHLAQTLARMLNVPFAIADATALTEAGYVGEDVENILLKLIQAADYDVKRAEQGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTVANVPPQGGRKHPHQEFIQIDTTNVLFIVGGAFAGLDRIIESRVGQGGVGFGAHLRSISERDTDDIFSKVMPEDMLKFGLIPEFIGRLPVITTVRNLDREALIKILTEPRNAYVKQYQRLFELDGVELEFDTGALEAIADQAMLRGTGARGLRAIMEEVLLSVMYEVPSNPDAARVLITREVVVENVIPTIVPREFVGRRARREREEKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSN5</accession>
    <name>U5VSN5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357584.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01760"/>
    <dbReference type="EnsemblBacteria" id="AGZ38641">
        <property type="protein sequence ID" value="AGZ38641"/>
        <property type="gene ID" value="AFR_01760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.355"/>
    <dbReference type="eggNOG" id="COG1950">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120441_0_0_11"/>
    <dbReference type="OrthoDB" id="1925503at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007165">
        <property type="entry name" value="Phage_holin_4_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37309">
        <property type="entry name" value="PTHR37309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04020">
        <property type="entry name" value="Phage_holin_4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="128" mass="13493" checksum="FA832ABA1A02AFEB" modified="2014-01-22" version="1">MGILIRLVITAVSLWIATLLIGGIRLTTDSIGGQIGTLLAVAVIFGIVNAVLRPIIKTIGCGLYVLTLGLISLVINGLLFLLVSWIAGQFDLPFHVDDFWPSAVLGALLVGVVSWVLNMLVPDGGDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSS0</accession>
    <name>U5VSS0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359564.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08720"/>
    <dbReference type="EnsemblBacteria" id="AGZ40033">
        <property type="protein sequence ID" value="AGZ40033"/>
        <property type="gene ID" value="AFR_08720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1774"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="16678" checksum="A072B4EB17A3BC8D" modified="2014-01-22" version="1">MSDGFVTERYLHLRRAAFLMCGDWALADDLARTTLARVLTELDVTDPELWSYADLMSAFKKPRGRREHVFVAPSDPATSTGGDLHTVLVLDALHGLHPRCRAVLVLRHFCGLAIDETADALDLDDSRVLAFEAEGLGAFESLLNAARTAGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSS9</accession>
    <name>U5VSS9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Periplasmic iron-transport lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359398.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07890"/>
    <dbReference type="EnsemblBacteria" id="AGZ39867">
        <property type="protein sequence ID" value="AGZ39867"/>
        <property type="gene ID" value="AFR_07890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1611"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_1_1_11"/>
    <dbReference type="OrthoDB" id="1942521at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666029" evidence="1">
        <location>
            <begin position="30"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="67"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="36444" checksum="E01D43BE84BE279F" modified="2014-01-22" version="1">MYRRPLGWRRALTVVAAGLLAATTVTACAADEDEAAAPAASGSAAAAFPVSIEHKFGSTTIEEQPERVVTVGWNDQDPVLALGVVPVSTREWFTEYPAYPWVKDALGGKQLTTFSAELNFEAIIKQQPDLILAIYETINKETYDKLSQIAPTVIQSSAYADEQTPWDVQTLTTGKALGKSAEAQALVDKVNTKITEAKTANPQFAGKTLVVDFGPEKGQHWLIPAKDPRRALFDALGFAAQKESEDVSEERLDLLDEDVLFVNGATKKDMLTSPAFNRLKVVTEDRALYTSFSTPLGGALAYSGPNALLYALDMLVPELKNATDGDKTTAVTDLSDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT01</accession>
    <name>U5VT01_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359525.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08525"/>
    <dbReference type="EnsemblBacteria" id="AGZ39994">
        <property type="protein sequence ID" value="AGZ39994"/>
        <property type="gene ID" value="AFR_08525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1734"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_7_2_11"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="22978" checksum="63179016BF8B11AA" modified="2014-01-22" version="1">MTLLDLDPDQLLSTTRAVRKRLDFTRPVPDDTIRECVAMALQAPSGSNNVTMRFVVVRDEAKRRAVGDIYREVYAGYKASPGYAGKPTGDDARDAVQRRVASSADYLGEHMGDAPVLVLACNEGPNRQWAVAGMGNVIPATWSFMLAARARGLGTAWTSMHLARERDVAEILGLDYETLAQAVLTPVAYTKGTDFRPAGRPAPDEIIRWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT19</accession>
    <name>U5VT19_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357739.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02535"/>
    <dbReference type="EnsemblBacteria" id="AGZ38796">
        <property type="protein sequence ID" value="AGZ38796"/>
        <property type="gene ID" value="AFR_02535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.510"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009153_0_0_11"/>
    <dbReference type="OrthoDB" id="1936977at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1164"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1249"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1257"/>
            <end position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1279" mass="122349" checksum="1A3EF282DB3677F2" modified="2014-01-22" version="1">MPPEDPSGSDAWTDPAEEGATFGQTNVTTTNLGDFEDWAREDPPGWRKLKAAVNGGSAMSTADAEAVALDRVDPQSLATAAGAFQQAYDTLSYLETFVRDHSKAIAGPDRAWQGVAADAFIAKMEYLADYLGNQAERIAGGDGTGSESSVPNQLLKSANYLAWAQSTMDYLDVAWANIAAANGANHTDEGLVAISSTKFARPMTDQMLEVVRTLAGQYELTYNKVQHPGGDGSPEFTTPDPKNITTPVLSSITTPLFTTPNITTPPPLNITTPPPVDITTAPPPNITTPPPITPPQVSPPPSGSVPPPGGENFVTPPPVNGPGGPVSPPPVNGPEAFEAPPIGEGPGGGGGGPVDSFAAPEIPGGPEGVNAPQVDGPGGLPGFDSQNLTNPDGSPITDPQGVNAQNNAPIVPPVVPAPPASGGGSDNNRRAAPPPVADLPDSPGAGGGVGNFNPPEIPGAPGSGGGGVNAPEAPGGGAFDGVDVPGVNAPNANAPGFDAPGVGSPGGGMPMMPGSPGGGAGGGNSGAPERPDASGLVGGDPSDWAPPGAGTGFDVPNAPGGANPGGAGLGMPGGGMPMMPGSPGGGGAGNGNGIPERPDASGLVGGDESDWSAPGAGGVDIPDAPGGATPGGAGLDVQSPSVSGPGETGQFPGAGQVPGGGMPMMPGSPGGGGAGNGNGNGIPERPDASGLVGGDESDWSPSGAGGVDIPDAPGGATPGGAGLDVPTPSLTAPGETEGFENSPSAPGMPMMPGMPGGGSPGAGGNNGLPERPDASGLVGGDASDWSPSGAGGGVDIPDAPGGATPGGAGLDVPTPSLTAPGETEGFENSPSAPGMPMMPGMPGGGSPGAGGNNGLPERPDASGLVGGDASDWSPSGAGGGVDIPDAPGGATPGGAGLDVPTPSLTAPGEGSSPGAGMPMMPGMPGGGSPGAGGNNGLPERPDASGLVGGDASDWSPSGAGGGVDIPDAPGGATPGGAGLDVPAPSLTTPVEGSSSAPGAGMPMMPGMPGGGSPGAGGGNGIPERPDASGLVDGDASDWSPSGTGDGVDIPGAPGGAIPGGAGLDAAVPSPAVPGDWFDSSPSAPSSGMPAQGEPSSGNGTPGVAELVEGDTSDWQPGETAAGGVPGMPMMPGAGAGEKAAAAERPDAAGLVEGDGEMWEPEGVAEVPSAGAPPPPVDPASGWHQGVSLPEAGVPVPGMMPVLPGAGERDEAERPDAAELLEEQVVTWAGAEGEDRVPVVQPAEVAEDVAGWDDTGDAWWLRGDEDTGHEERATDARLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT82</accession>
    <name>U5VT82_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inner-membrane translocator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_03255</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357883.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03255"/>
    <dbReference type="EnsemblBacteria" id="AGZ38940">
        <property type="protein sequence ID" value="AGZ38940"/>
        <property type="gene ID" value="AFR_03255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.655"/>
    <dbReference type="eggNOG" id="COG1172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028880_0_0_11"/>
    <dbReference type="OrthoDB" id="1254678at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="329" mass="34017" checksum="2768F18E2EDB1537" modified="2014-01-22" version="1">MSELKRKLAGWDTIIIALTVVVLVIASLGVPNFGTSRNFTFLVLDLMPILLVALPMTFIIVTGEIDLSVASTLGLASSIMGWLWNQGLSIESIIPICIIAGAVLGALNGFLVTGLGLPSLAVTIGTLALYRGLALVVLGDGAVADFPFNYTGWVTGTIGGGSIPNVLILIVALAVIFGVVLHATPFGRSLYAIGANAEAAHFSGIRVARTKFWLYVVSGAVAGLVGVLWTLRYSSARADNGAGLELAVVAAVLLGGVSIFGGKGSLPGVLAGVVLLAALQNALRLQDVSGQALNIVTGALLVLSVLLPNIATSIRSTWQRRRLRRAATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTC6</accession>
    <name>U5VTC6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD linked oxidase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03505</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357933.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03505"/>
    <dbReference type="EnsemblBacteria" id="AGZ38990">
        <property type="protein sequence ID" value="AGZ38990"/>
        <property type="gene ID" value="AFR_03505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.704"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_410864_0_0_11"/>
    <dbReference type="OrthoDB" id="330793at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="2">
        <location>
            <begin position="330"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="739" mass="79063" checksum="AA9A4C40FDEB195C" modified="2014-01-22" version="1">MNYGHPLKFGVFITPGSTAPGEPVALAQRAEAFGYDLVTFQDHPYQPRFLDTWTLLSWVAGRTERIHLAANVLNVPLRDPAVLARSAASLDLLSGGRLDLALGAGAFWDAIEAMGGRKLSPGQAVEALEEAIDVVRGVLDAGEPGPLRHLGEHYRIPAAQRGPLPAHRIPLWLGGYKPRMLRLTGRKADGWLPTLSAFKPGEFAAANKIIDEAALAAGREPAEIRRLVNITPDRTVDELLPLVLEDGVGTFIVMTDDAATLERFAAEIAPALREAVARELPEAPTAVFRTTAVRAKRRTGISYDTVPASLAEAAVEPGDLDYARVRSTYMRGGAPGLVLRASSTAEVVDALAFVREHPGVPFAVRSGGHGISGRSTNDGGLVLSLAALNTIEVLDTERRLIRVGAGARWTDVADALLPYGWALSSGDYGGVGVGGLATAAGVGWLARKHGLTIDHLRAVEVVLADGSVVRASEEENPDLFWAVRGAGANFGVVTSFDFEVDEVGDVGWGQFVVGADDPADLLVRWGAAVEAAPRDLTANLIMGRPRPGQPGYAQIMAMVDSSDPQTIIDQLTPVASVGPLYQQNVVLTPYAGVMANVAEGYHQGQGEPVARSGLIEHLTPEFAAAAAELIASGAIHWFQIRAVGGAVADVPEDATAYAHRSANFSIVVMGSSRRRVDPLWDALAAHFDGLYLSFETDLRPERVADAFPPRTLERLRELKHRYDPQNLFRDNFNIEVGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTG2</accession>
    <name>U5VTG2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoyl synthase</fullName>
            <ecNumber evidence="6">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Lip-syn</fullName>
            <shortName evidence="6">LS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Lipoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Lipoic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Sulfur insertion protein LipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lipA</name>
        <name evidence="8" type="ORF">AFR_09225</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09225"/>
    <dbReference type="EnsemblBacteria" id="AGZ40134">
        <property type="protein sequence ID" value="AGZ40134"/>
        <property type="gene ID" value="AFR_09225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1878"/>
    <dbReference type="eggNOG" id="COG0320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033144_2_1_11"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102552">
        <property type="term" value="F:lipoyl synthase activity (acting on glycine-cleavage complex H protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102553">
        <property type="term" value="F:lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="PTHR10949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="67"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="34241" checksum="5F6F253FAE37C353" modified="2014-01-22" version="1">MTIAPEGRRMLRIEARNAETPIERKPPWIKVKAKMGPEYTHMRGLVQKEGLHTVCQEAGCPNIYECWEDREATFLIGGDQCTRRCDFCQIDTGKPAEFDADEPRRVGESVATMGLKYATVTGVARDDLPDGGAWLYAETVRQIHKLQAGCGVELLIPDFNAEPDQLAEVFGSAPEVLAHNVETVPRIFKRIRPGFRYDRSLGVITQARAAGLVTKSNLILGMGEERAEVSQALRDLHAAGCELITITQYLRPTPRHHPVERWVKPEEFVELRDEAEEIGFAGVMSGPLVRSSYRAGRLYQQALAARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTM3</accession>
    <name>U5VTM3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10295"/>
    <dbReference type="EnsemblBacteria" id="AGZ40348">
        <property type="protein sequence ID" value="AGZ40348"/>
        <property type="gene ID" value="AFR_10295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2097"/>
    <dbReference type="eggNOG" id="ENOG5033A40">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084749_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019051">
        <property type="entry name" value="Trp_biosyn_TM_oprn/chp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09534">
        <property type="entry name" value="Trp_oprn_chp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="17713" checksum="394D0C695D5583A7" modified="2014-01-22" version="1">MTSTVLFCLAGAGLAVYAVTRTWSEVVTERPGLSALRAIRTGADLEPWVIGLALVALAGAGALLATRGIVRRVLGGLLALAGLGVAAGAIAGRAGLDTGSAGAGGVIWPVLCVLGGLLIVLGGLSAVRYGHEWPAMGARYERRVSADQVEVDQRVDTRAVWEALDRGDDPTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTT9</accession>
    <name>U5VTT9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Endoribonuclease L-PSP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357986.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39041">
        <property type="protein sequence ID" value="AGZ39041"/>
        <property type="gene ID" value="AFR_03760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.758"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_4_2_11"/>
    <dbReference type="OrthoDB" id="1850770at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="PTHR11803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="131" mass="13653" checksum="BAD2A2539F87A74B" modified="2014-01-22" version="1">MNRSAVNPWTWSAALGYNQGEIVSGHTRTLYCAGQTAMSSDGKPQHPGDMAAQVALSLDNLEAVLGEAGMSLANLVRLTVCTTDVDRLLEHYGVLASRLAAAGIAPATTMLGVTRLAIPELMVELEGTAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUV7</accession>
    <name>U5VUV7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DSBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360553.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11390"/>
    <dbReference type="EnsemblBacteria" id="AGZ40567">
        <property type="protein sequence ID" value="AGZ40567"/>
        <property type="gene ID" value="AFR_11390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2322"/>
    <dbReference type="eggNOG" id="COG3917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087602_1_0_11"/>
    <dbReference type="OrthoDB" id="996965at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DSBA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="20907" checksum="3D3015952A81B816" modified="2014-01-22" version="1">MEATYFFDPACPFTWRTSRWLVAVAPDRDVTVHWRAFSLSILNGDNVPDQYKPMMAASSRALRLVEALRADHRDETIGAFYTELGTRTHDAGEPLSDDIVVAAAEAAGVADPKTVLDDASWDEAVRESHELAFGSAGPGIGSPVLLIEGATRGLHGPIIDTVPDREEALAIWDATVPLARSTTFFELKRGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUX1</accession>
    <name>U5VUX1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11465"/>
    <dbReference type="EnsemblBacteria" id="AGZ40582">
        <property type="protein sequence ID" value="AGZ40582"/>
        <property type="gene ID" value="AFR_11465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2337"/>
    <dbReference type="HOGENOM" id="CLU_1754918_0_0_11"/>
    <dbReference type="OrthoDB" id="2073415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="15367" checksum="35C54BC0179C38FC" modified="2014-01-22" version="1">MVTAVLLILGALSPLLMREPEQPANPVALPVISIPPLPTVIATPAPVGSPGVLRAVRQARKATPAPVLLGPADKAGLPDLVGAYCRATYGGLTLAASTADGWVCARLGQGNTAVDMDAMCRHRYGAAAWADLGDESDPRSWRCYRDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUX4</accession>
    <name>U5VUX4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12535"/>
    <dbReference type="EnsemblBacteria" id="AGZ40793">
        <property type="protein sequence ID" value="AGZ40793"/>
        <property type="gene ID" value="AFR_12535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2546"/>
    <dbReference type="eggNOG" id="ENOG5032I9B">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188864_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="89" mass="9481" checksum="47BF3BFD15F140E6" modified="2014-01-22" version="1">MIAASRFVTAHALFEGRADLEGYPFQLLGVVSQRGTGTENLSAALAGAEMLIPYGWELVNVSEFTGSNHACAVMRRKAARFPVAGEPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV13</accession>
    <name>U5VV13_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative multi-sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359027.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06035"/>
    <dbReference type="EnsemblBacteria" id="AGZ39496">
        <property type="protein sequence ID" value="AGZ39496"/>
        <property type="gene ID" value="AFR_06035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1226"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_44_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="482"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="705" mass="74787" checksum="CCFCE87657F7A059" modified="2014-01-22" version="1">MLTVDEALLRDPSRLAAVADLRPALAALPVLLDSVAAVAARSLAAPIALVSLVGESQEYLYGTYDPAGALAGRETIPLATSMGKYVLGANGCVRSTGVSPAARFGVSAFAGVPLKDHGNRPVGCLAVLDTENRRWSAGQTSLLSELARILSPEVLASIAPTAPTAAADAAEDNDRFLAAILDSLAVGVIACDEAGKIVLVNRALRDVQSLPGDRVPEDYAERAETTLVHSDMTPMSWQQTPLMRALRGEHVRDSEVLVQAPGHRLRTFATNAQPIIGAGGRRLGAVAAAHEVTAMRRAEHFRQCHLAVAHILAASDSPEQAAFGVLRVVATTLRWPYAEMWLIDEATDSLRSIGHWSEPGLKAGKLSRRAVTKGVGVTGRTWATGRPVWIHDITSEAAAEPHPRIEACLRLGFRTVLSVPVRDGDTMLGVLTCYSDAPEHHEDLLTVLLDGVAAQFGVFVALRRAAALAQELDRAKDDFIALVGHEMRTPLTTIAATAGMLREEAESFEPETQHMLAAIHRNTILLQDIVGKLLDLAGLEAGHIGLTIAPVDLVEVITEAMTAARPAASANGLTLIAELPDRLTVEADAGRFRQVVDDLLSNAVKYSPGGGDIHVRLSADDTTAELVITDGGIGVLDTEREHLFDRLYRGSNVRHQGTTGSGLGLSLARTIVELHGGTITLTGRETGGTTVQVRLPLQATSPSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV63</accession>
    <name>U5VV63_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, PadR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359111.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06455"/>
    <dbReference type="EnsemblBacteria" id="AGZ39580">
        <property type="protein sequence ID" value="AGZ39580"/>
        <property type="gene ID" value="AFR_06455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1309"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089258_1_4_11"/>
    <dbReference type="OrthoDB" id="1629615at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018309">
        <property type="entry name" value="Tscrpt_reg_PadR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10400">
        <property type="entry name" value="Vir_act_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="7"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Vir_act_alpha_C" evidence="2">
        <location>
            <begin position="92"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="184" mass="20992" checksum="6D4DD5D9251485FA" modified="2014-01-22" version="1">MSLRHAVLAALLEGEASGYELAKRFDVSVAEFWSATPQQLYRDLDRLETDGLVDARVVEQHRRPNKRVFSLTEAGLAELHTSTTEPARPPAIRDELMVKMQALDTGDQQTLRATVAARLTRSTAKLARYDRMRDKLLNGRDEDTYLRDSNRIGPYLTLMAGRMYEQAYITWAERTLAILDARQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVH4</accession>
    <name>U5VVH4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360866.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40807">
        <property type="protein sequence ID" value="AGZ40807"/>
        <property type="gene ID" value="AFR_12605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2560"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_0_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="30398" checksum="3B40BE38867CD809" modified="2014-01-22" version="1">METDIGAFLRTRRDRITPDQVGLPAGATRRRVPGLRREEVALIAGVSPDYYVRLEQGRTAHVSDQVLCAVARALSLSGAETEHLRNLARLPEPAPSLLQKSATAEDEPLARLLDAITDAPALLVDTRMNILAANAAAEAVFDVSGMAQPPNSARQLFLAREAKARYGNWEAAAEEVVAHLRLMTGRWPDDAPLTALIDELGHHSDDFRRLWARADVGEKRVGTVHVTNHVAGPLEFDYHVLTVPARPDRSLLTYLPRPGSPTQEALRMLLTRIPAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWD6</accession>
    <name>U5VWD6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_14435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361228.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14435"/>
    <dbReference type="EnsemblBacteria" id="AGZ41169">
        <property type="protein sequence ID" value="AGZ41169"/>
        <property type="gene ID" value="AFR_14435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2929"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040011_1_1_11"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="164"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="404" mass="41948" checksum="FD07F91804A990C8" modified="2014-01-22" version="1">MTTTAQPERVGRSWIGLLSLANLGLWMGYFGPLQVLLPNQAQDLAGVDKTTALGIITGAGALVAVLVGPIAGALSDATTARSGRRHTWIVAGALLGCAGLALLSGQRTLIGLTLAWCVAQAGLNTLQAGLTAIVPDRTPVRQRGLVSGWVGLTQSIGVVVGVLLVTVVAGGYFLIAVLVALTVLPFVLATPDPAVGAGAAPRVTLRRLAGVFRFDPRAHPDFAWAWATRFLVQLGNAMATLYLLYFLRDRVHHGNPEDGLLLLILVYTATTVLTVVIGGVVSDRTGRRKPSVILSGYVMAAAAGLLALWPTWTGALIAAAVLGLGFGVYLSVDQALITQVLPAEQDHAKDLGIINIANSAPQVLGPALAFPIVAYLGGYTVLYLVVAAVTVLGSVLVTRIRSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX59</accession>
    <name>U5VX59_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360072.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10130"/>
    <dbReference type="EnsemblBacteria" id="AGZ40315">
        <property type="protein sequence ID" value="AGZ40315"/>
        <property type="gene ID" value="AFR_10130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2063"/>
    <dbReference type="eggNOG" id="ENOG5031T2E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172632_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="106" mass="12184" checksum="58110E20E01DF7C7" modified="2014-01-22" version="1">MSKERAKRRTERLAVLEKEKAARARRSNRRQRRRALVRSLKPGRRPSSGRLYRRSRTQRTGIIVVPLIVAVLVWFVVPDLALRIVLIAMIALVLPAVVVVVLGRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXH4</accession>
    <name>U5VXH4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360309.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10730"/>
    <dbReference type="EnsemblBacteria" id="AGZ40435">
        <property type="protein sequence ID" value="AGZ40435"/>
        <property type="gene ID" value="AFR_10730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2187"/>
    <dbReference type="eggNOG" id="COG3154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070584_1_0_11"/>
    <dbReference type="OrthoDB" id="1713977at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017517">
        <property type="entry name" value="CHP03083_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010872">
        <property type="entry name" value="MDMPI_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024344">
        <property type="entry name" value="MDMPI_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07398">
        <property type="entry name" value="MDMPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11716">
        <property type="entry name" value="MDMPI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03083">
        <property type="entry name" value="TIGR03083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MDMPI_N" evidence="2">
        <location>
            <begin position="16"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="MDMPI_C" evidence="1">
        <location>
            <begin position="150"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="26331" checksum="765D3B2268857691" modified="2014-01-22" version="1">MTQLPPFPELLDLIAERSATFRSAFATTGDLGLRVPGCPDWTLRDLVVHLGQVQRSWAVRVAAGPADKPATVTHEEPTGDLGEWSADSTRVLLDALRAAGPDRDCWTWWGESEAPMTSGAVARHQVQEAAVHAWDAQETIGKPEPIPAGVAVDTVDEFLVVTLGSLGAWPHRPARFALSAVEGPAWTLDLTPAGVKSGPAASGEPLATLRGSASDLVLALYHRIPLDSLDVDGDRKAVEELLAWAGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXI6</accession>
    <name>U5VXI6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41683">
        <property type="protein sequence ID" value="AGZ41683"/>
        <property type="gene ID" value="AFR_17025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17025"/>
    <dbReference type="HOGENOM" id="CLU_2784534_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="68" mass="7658" checksum="83822A76F3235ABC" modified="2014-01-22" version="1">MSRPRACSDDTLLLVVQLVRAGWSQRAISEVLNGLDIPTPNDCSHWYASYVCRLLQTRDGRRLLAVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYA3</accession>
    <name>U5VYA3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cystathionine beta-synthase/cysteine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18475"/>
    <dbReference type="EnsemblBacteria" id="AGZ41973">
        <property type="protein sequence ID" value="AGZ41973"/>
        <property type="gene ID" value="AFR_18475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3749"/>
    <dbReference type="eggNOG" id="COG0031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="36"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="357" mass="39084" checksum="A31AF39BD2096393" modified="2014-01-22" version="1">MNATTALFDEITEAQMMPRLVRLGPNLYGAVFTLMKLLPARYILRRAIERGELEPDTAVVETTSGTFGLALAMQSALLERELILVSDPAIDVHLHRRLNDLGAQVEICRDPAPVGGYQRSRLDRLAEVRAERERTFCPEQYTNPDNPRSYGVVADQLARTLGQVDAVIGPVGSGGSMCGTVRGLRATTPHTRAIGVDTHHSVLFGQPDGHRSLRGLGNSLWPENLDHSVFDDVHWCTAGEAYAATRRLHGRHALYQGPTSGAAYLVADWYARRNPGELCVVMLPDEGYRYQATAYDDGWLAANGYSLGDAPAGPVETATPERPADAWSWLRWNRRSYDDIPGAVARTAGPVPAEAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYL6</accession>
    <name>U5VYL6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362357.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18530"/>
    <dbReference type="EnsemblBacteria" id="AGZ41984">
        <property type="protein sequence ID" value="AGZ41984"/>
        <property type="gene ID" value="AFR_18530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3761"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1178229_0_0_11"/>
    <dbReference type="OrthoDB" id="402396at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="235" mass="25183" checksum="D748D3F37F1ABEF1" modified="2014-01-22" version="1">MTTLDQLLGDPYDAANPYGFAAIGAGAALPAGSGTGEDLRALYRRSPRAARAAHPAVDDRRRLGAAIGAVDSGLRITLRHLRARRLYDRPATEIPYLRSALAGVLADLLLCDRLLAAADLRVARGLVPQALLGAMDGLSVLLGSRFYIRVGEQAIFPVLFRETQDDLLALGTAGPAPVTDVRALLPGVDAPALHRDRGGWRTPPGPAADLVDDLFDRYHGNRSFDLSRRPLPDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZJ5</accession>
    <name>U5VZJ5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inositol 2-dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Myo-inositol 2-dehydrogenase</fullName>
            <shortName evidence="1">MI 2-dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">iolG</name>
        <name evidence="4" type="ORF">AFR_20730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the oxidation of myo-inositol (MI) to 2-keto-myo-inositol (2KMI or 2-inosose).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>myo-inositol + NAD(+) = H(+) + NADH + scyllo-inosose</text>
            <dbReference type="Rhea" id="RHEA:16949"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:17811"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362790.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20730"/>
    <dbReference type="EnsemblBacteria" id="AGZ42418">
        <property type="protein sequence ID" value="AGZ42418"/>
        <property type="gene ID" value="AFR_20730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4203"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_0_1_11"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050112">
        <property type="term" value="F:inositol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019310">
        <property type="term" value="P:inositol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01671">
        <property type="entry name" value="IolG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023794">
        <property type="entry name" value="MI/DCI_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="3">
        <location>
            <begin position="135"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="35958" checksum="218D07C6F20B5C23" modified="2014-01-22" version="1">MLKVGVIGTGMIGQDHIRRMTAVLAGVTVTAVTDVDVETAKKVAGGLPGATLHLTGEDLIADPAVDAVLVCSWGPTHEQYVLASIAAGKPVFCEKPLATTQDACLRIVEAEVAAGRRLVQVGYMRRYDAAYRALKRVVDSGRIGVPLMMHCTHRNAGVPSFYEKESAITDTAVHEIDMVRWMFGEEITAAQVLIPRKSRNGGPLQDPLILILELAGGAIVDVEISVNIRYGYDIRGEVVGEDGTAALGEFSPVVVRAAGERSAPIPLDWRERFLLAYDVEIQEWIDSVAGGGEPVGPSAWDGYAAAVVSDAGVAALRSGSRVPVTLAAQPTFYRAPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W099</accession>
    <name>U5W099_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361469.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15640"/>
    <dbReference type="EnsemblBacteria" id="AGZ41410">
        <property type="protein sequence ID" value="AGZ41410"/>
        <property type="gene ID" value="AFR_15640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3176"/>
    <dbReference type="eggNOG" id="ENOG50322TY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253714_0_0_11"/>
    <dbReference type="OrthoDB" id="2139820at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="23319" checksum="968E2C6425733CAA" modified="2014-01-22" version="1">MDEHLFGFLLRRIAPLVVAPVGSLPPAGFQLRGVVRAGHTRARVVFSPDARRESGIGYEFTLTEDGVDAAIDWLRITVSAGVTPTGGVVDSHGNTVGDAAEHGFRPTGLNRTDDHNVFSLLSTLIHGGGSPWFEDEYTCAGFMLGPSGACRVYVRVAATGEVYGLQLPMEEADGTNVSGKAGSLPSVLGSLIDNGTLLTLPVVDTADDYCRIVYDLGDWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0C4</accession>
    <name>U5W0C4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extracellular matrix-binding protein ebhB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361440.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15495"/>
    <dbReference type="EnsemblBacteria" id="AGZ41381">
        <property type="protein sequence ID" value="AGZ41381"/>
        <property type="gene ID" value="AFR_15495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3146"/>
    <dbReference type="eggNOG" id="COG1876">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048073_0_0_11"/>
    <dbReference type="OrthoDB" id="1587222at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003709">
        <property type="entry name" value="Pept_M15B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02557">
        <property type="entry name" value="VanY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665546" evidence="1">
        <location>
            <begin position="34"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="VanY" evidence="2">
        <location>
            <begin position="236"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="351" mass="36216" checksum="46B2537636C60BAA" modified="2014-01-22" version="1">MTTRHGSRLKPLAAAAAAAGALIIAGPAAPASAAVPKAAQQWASLMHRSLTPTPQVLALREKLAGQRATLVTRSAGVTKSKTTQTGAQTALSAAITADGTARTRYAVAVEALTTAKNARTVALQQRPRNATKVSRAQAAVTAAAKTVTASRTAAAAAARTLKTAQGTARTATADLDTAIASWRVMSEAVATNQERLAGLNKAPEYATQAAALSRDVVAQVRPVFTTADTTAVNGVTVHKSVSFAFREMLAAAKADGVVLSGGGFRTKQRQIELRKINGCPDVYTAPASSCRVPTAIPGRSLHELGLAVDITQGGSSLTSSSAGFRWLSVHADEYGFVNLPSEPWHWSITGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0J0</accession>
    <name>U5W0J0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptide/nickel transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_22365</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363117.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22365"/>
    <dbReference type="EnsemblBacteria" id="AGZ42743">
        <property type="protein sequence ID" value="AGZ42743"/>
        <property type="gene ID" value="AFR_22365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4534"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_5_2_11"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="83"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="29889" checksum="8C9B28D174FBD981" modified="2014-01-22" version="1">MTIATERPLAGRLSISLRRPRPGVAVATGFLGLILVAVLWPGLLAGDPLAADPFHVLQAPSATHWFGTDQLGRDVFDRVVHGARHSLAIGALATLIAVTAGILLGVLAGLSHRYADEALSRSFDALSAFPLLLLALLFIAVAGPGTVSLVVAIGIATLPHYARVVRAQTFVVRQAGYVTQAVAYGDSRTRLVLRHVLPNVLGPIPVLAMIGLGEAVLAAAGLSFLGLGPQPPSPEWGAMLSEGRGYLRIAWWASVLPGLVVTATVIALTVTGRHLQRRFEGRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0R2</accession>
    <name>U5W0R2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21560"/>
    <dbReference type="EnsemblBacteria" id="AGZ42582">
        <property type="protein sequence ID" value="AGZ42582"/>
        <property type="gene ID" value="AFR_21560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4372"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="151"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="23978" checksum="AF8776212CC057AB" modified="2014-01-22" version="1">MTAIRVLLADDHEFFRSGFRSALETQPDLECVGDVGDGHAAVTEIERLRPDVAVLDVRMPRMDGLTAAETVLSRGTGTRIMLLTTYDDDSYVYRGLRAGTSGFCLKSMPVEELLNAIRVVARGDALIDPSVTRRLVTRFANGLAPAAVHEPPPDLDRLTAREREILLEVARGHSNAEIAGRLYVGEQTVKTHVSHLLTKLGLRDRVQAVIYAYENGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0S9</accession>
    <name>U5W0S9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41545">
        <property type="protein sequence ID" value="AGZ41545"/>
        <property type="gene ID" value="AFR_16315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16315"/>
    <dbReference type="HOGENOM" id="CLU_2857518_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="64" mass="6521" checksum="248F3FA76CB51721" modified="2014-01-22" version="1">MTVVMTAPGAAVREGAIGMRVAMTPPGRAIRDGSIGMRSCLAPRGSSGSFRTEGCDDRAGPRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0W1</accession>
    <name>U5W0W1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phosphosugar isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363196.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22545"/>
    <dbReference type="EnsemblBacteria" id="AGZ42779">
        <property type="protein sequence ID" value="AGZ42779"/>
        <property type="gene ID" value="AFR_22545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4570"/>
    <dbReference type="eggNOG" id="COG2222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_4_0_11"/>
    <dbReference type="OrthoDB" id="1046291at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="25"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="295" mass="31463" checksum="E07DF1D74BC329E4" modified="2014-01-22" version="1">MNDTSEEIASQPAVWEQGLALTDQARRELAAPGERMLVLGCGTSWFVAQSIAELRESAGLGETDAVCASEYVPRRRYDRVVAITRSGTSTEVLDALRQVPEGTHRVAVTAVAGMPVDTLAESRLLLDFADERSVVQTRFPTTVLAIARAAFGEDLSHLIADGHAALDAPLPADPAAIDHLVFLGTGWTVGLAHEAALKVREAAQAYSESYPAMDFRHGPVAVAGTRSLVFSFGAVPIGLDEVGRSAGATTYRDERDPLAQLVLAQRFALALAAHRGLDPDRPRLLTRSVVLTDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Y5</accession>
    <name>U5W0Y5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363048.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22035"/>
    <dbReference type="EnsemblBacteria" id="AGZ42677">
        <property type="protein sequence ID" value="AGZ42677"/>
        <property type="gene ID" value="AFR_22035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4466"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_17_1_11"/>
    <dbReference type="OrthoDB" id="1445278at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="23861" checksum="1682770FE1EAF277" modified="2014-01-22" version="1">MLKRLAVVVPAHNEEGLLPDCLEAIEAAAGRFEGRTEVVVAADDCDDGTATVAEAAGAEVVRLAARRVGAARAAGFAHALRHGTDGLWLATTDADSLVAPDWLLWHAGHAARGTEVLVGTVGVDDWTPWPVTVRDAYERHYRAALRHVHGANLGCDARAYLRLGGFAAVPHDEDVDFVRRAVAGKLRVRYDAGSPVITSSRRHARAPSGFAAHLADLADEVSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W132</accession>
    <name>U5W132_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrate/sulfonate/bicarbonate family ABC transporter periplasmic ligand binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363093.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22260"/>
    <dbReference type="EnsemblBacteria" id="AGZ42722">
        <property type="protein sequence ID" value="AGZ42722"/>
        <property type="gene ID" value="AFR_22260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4512"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_2_1_11"/>
    <dbReference type="OrthoDB" id="785019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBPb" evidence="2">
        <location>
            <begin position="29"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="32770" checksum="16EC1ADC254A584A" modified="2014-01-22" version="1">MRRALIPVVALLLALTGCGGGDSESDRVSLTLGDQANGLKTLFEASGALEGASFDHKWAEFQGAAPLFQAMQSNNVDTGTAADIPTLQAISGGLPIKFVAASQSNGQGTAILLRKDSAIKSVAELKGKEILVSTARGSIADYLLANVLQKAGLKYSDVTVKYAVPTAAQAAFSSGKVEIWAIFGVFQATAVAQGAKVLIDGRDGGTSGIGVISASDKSLADPKRKAAIADFLKRLSTANTWAATHADEYAQVYSDKNGVPLDVAKVVVSWGSTALLPVTPDVIAKVQPVSDLMRGVGVLPVDVKVADHVDTTAFSTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1W7</accession>
    <name>U5W1W7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methylaspartate mutase E chain family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362353.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18510"/>
    <dbReference type="EnsemblBacteria" id="AGZ41980">
        <property type="protein sequence ID" value="AGZ41980"/>
        <property type="gene ID" value="AFR_18510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3756"/>
    <dbReference type="eggNOG" id="COG4865">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029922_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050097">
        <property type="term" value="F:methylaspartate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019670">
        <property type="term" value="P:anaerobic glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00245">
        <property type="entry name" value="Glm_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006396">
        <property type="entry name" value="Glu_mut_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06368">
        <property type="entry name" value="Met_asp_mut_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001495">
        <property type="entry name" value="Met_asp_mut_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="415" mass="43887" checksum="740A9A6ED77928BB" modified="2014-01-22" version="1">MTAAAPFGEFVTRAQSRGALVVQPRMGFGDPALMREGLAATKAAAATTVGTLTLDSYTRVGDDESVARALRENAPLNGYPIVALPRATSLAVLDGLRDATFPVQVRHGSPRPEHIFAALIELGLDATEGGPVSYCLPYSRTPLRDAVASWRRSCLMLADTAGHGVVPHLESFGGCMLGQLCPPGLLVAISLLEGLFFAQHGLRSISLSYAQQQNPGQDRQALAALRRLAADLLPGVEWHIVLYAYMGVYPRTHGGALRLLGAAAELAVTAGAARLIVKTAAEAHRIPTIADNVEALELAADRAAGVRRLPDQDGAEVYEEALALVEAVLSLDHDVGDALIAAFARGLLDVPFCLHPDNAGRSRSYVDDHGRLLWSDTGAMPIPASRAGHPARRLTATGLLTALSGMQHRYDEGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W351</accession>
    <name>U5W351_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362797.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20765"/>
    <dbReference type="EnsemblBacteria" id="AGZ42425">
        <property type="protein sequence ID" value="AGZ42425"/>
        <property type="gene ID" value="AFR_20765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4210"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_17_0_11"/>
    <dbReference type="OrthoDB" id="1339160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="7"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="144"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="388" mass="41121" checksum="B89A00303D3CD966" modified="2014-01-22" version="1">MADRSLGVAVIGAGMVGRAHANAYRNASTVFGTGPSPRLVAIADVHEPFATDAARRYGFSRTATDWRAVVDAPDIDAVSVAVANNLHREIVEAALAAGKHVLCEKPLAPSIGDAQAMVDAAAATDRVAAVGFTFRRSPAINAIREQVLHGGLGRVRHIVGNYWTDYGFDPQRPMSWRYLGGPGSGVLADIGSHLVDLAEFFCGPTTGVHGTSMSTLITSRRKPLGVAVGHSGGVELSDDLVPVDNEDICTFTTSYAGGALGTFSLSRVAYGHANSLKLDLFCENGTASFDLTRPAEFSVIDGTPAQEVNGARQVFIGPWHPYISRGLPMDFPTVGHGQNDFFVFQARSFLDQIAGVEQLPPCPDLAHGLHNLRVLDAVVSATDKPVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3A3</accession>
    <name>U5W3A3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21040</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362852.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21040"/>
    <dbReference type="EnsemblBacteria" id="AGZ42480">
        <property type="protein sequence ID" value="AGZ42480"/>
        <property type="gene ID" value="AFR_21040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4265"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_11"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="544" mass="58356" checksum="B91E9F4C22977E5E" modified="2014-01-22" version="1">MSATMIVRDLASGHGDRTLFSSLDLVVAPGDVIGLVGVNGAGKTTLLRTLAGLVPVEAGTVTLSPPTANVGYLPQERDRREGETVRDFLARRTGVGPAQAAMDAAAEALAEGAPGADDTYAAALERWLDLGGADLDERAEQTAGELGLTVALEHPMTALSGGQAARAGMASLLLSRYDIFLLDEPTNDLDLDGLRRLEEFVGGLRAGTVLVSHDREFLTRTVTSVLELDLAQQQVSLYGGGYAAYLEERDRARRHAREDYDEYADRKGALQDRAQMQRGWMENGVRNARRKAKDNDKIGRNVRAESTEKQAAKARQTQRMIERLEVVEEPRKEWELRMEIAAAPRAGAVVATLREALVRRGAFTLGPVTLQVDWADRVAITGANGSGKSTLLAALLGRLPLDGGSAQLGPGVVVGEVDQARGLFLGDEPLMAAFGAAVPEMSDADVRTLLAKFGLRAAHVLRPADTLSPGERTRAALALLQARGVNLLVLDEPTNHLDLTAIEQLESALASYTGTLLLVTHDRRMLEAVQTTRHFEVVDGKVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3G4</accession>
    <name>U5W3G4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycerol kinase</fullName>
            <ecNumber evidence="5">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycerokinase</fullName>
            <shortName evidence="5">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glpK2</name>
        <name evidence="5" type="synonym">glpK</name>
        <name evidence="9" type="ORF">AFR_27460</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Inhibited by fructose 1,6-bisphosphate (FBP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560091.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27460"/>
    <dbReference type="EnsemblBacteria" id="AGZ43753">
        <property type="protein sequence ID" value="AGZ43753"/>
        <property type="gene ID" value="AFR_27460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5566"/>
    <dbReference type="eggNOG" id="COG0554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_11"/>
    <dbReference type="OrthoDB" id="233605at2"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00186">
        <property type="entry name" value="Glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5 6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="7">
        <location>
            <begin position="5"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="8">
        <location>
            <begin position="265"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="82"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="249"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="416"/>
            <end position="420"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="505" mass="55257" checksum="454F41F13D075C77" modified="2014-01-22" version="1">MADFVGAVDQGTTSTRFMIFDHGGNEVGRHQLEHEQILPQAGWVEHNPIEIWDRTVSVIRTAMQKANLQASDLAALGITNQRETTVVWNRRTGRPYYNAIVWQDTRTDRIASALDRDGRGDVIRQKAGLPPATYFSGGKIQWILENVDGVREAAERGEAVFGNTDSWLLWHLTGGVDGGNHITDVTNASRTMLMNLETLEWDDELLSFFNIPRQMLPEIRPSSDPNTYGMAHAPGPLGGDVPLTGDLGDQQAAMFGQVCFAPGEAKNTYGTGNFMLLNTGTELVRSNNGLLTTVCYKFGDAAPVYALEGSIAVTGSAVQWLRDQLHIINAASESETLAASVPDSGGVCFVPAFSGLFAPYWRSDARGAIVGLSRFNTDAHIARATLESICYQSRDVVEAMAQDSGVTLDVLKVDGGITVNELCMQIQADVLGVAVSRPVVAETTALGAAYAAGLAVGFWKSTDELRENWNESRRWQPEWNDEQRADGYGRWKKAVQRTLDWVDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3H9</accession>
    <name>U5W3H9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27090"/>
    <dbReference type="EnsemblBacteria" id="AGZ43679">
        <property type="protein sequence ID" value="AGZ43679"/>
        <property type="gene ID" value="AFR_27090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5490"/>
    <dbReference type="eggNOG" id="COG0421">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068637_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="28624" checksum="8E8A3D434CDDE00E" modified="2014-01-22" version="1">MATHGNRRATIFTRVASGTASLEPDHSRERGWTLLVDGVPQSYVDLGDPTFLAFEYVRRLATVVRLSAPAGVPVKVLHLGGGGLTLARWVEATRPGSEQTVVERDGLLLALVSRSLPWPASVEVVVGDAREFTDSGPQHGYDVIVVDVFEGAAMPASVAGVGFAKSTAHLLRPGGVLAMNLTDVPPLSYSRIQAATLRAAFGDVALIAPPGMLRGRKAGNVVLAATHRPGVLNVERITRSVARDTEPAHVWYGAELNAFLAGARARLDGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3M8</accession>
    <name>U5W3M8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transport system inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_27365</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27365"/>
    <dbReference type="EnsemblBacteria" id="AGZ43734">
        <property type="protein sequence ID" value="AGZ43734"/>
        <property type="gene ID" value="AFR_27365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5547"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="90"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="313" mass="34166" checksum="D9C60C1EAC73E8C4" modified="2014-01-22" version="1">MAEQVLLRERPAGVQEKPVPARSRERLLQVLFLVPAVLYLLLFFGYPVAKNVLMSFQSYTTATFYTGEAPWVGLDNYTRALGSAVFDKALVNTVLFTVGSIAGQFVIGLALAVFFHRRFPLGGVLRSLLLLPWLIPLIVSGAVWRWILDQDNGALNRFLGATGVADRPGWLTSTSLALIAVIIVNIWIGIPFNTTILYGGLQDIPDELYEAAAVDGATGRRAFRYITWPLLRPVVGVVLVLGVVYTVKVLDIILGLTNGGPANATQTLATQSYHLSFQEFDFGQGAAVGNVLILISLAFAVLYLRATRRAVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W437</accession>
    <name>U5W437_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptide/nickel transport system substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22375"/>
    <dbReference type="EnsemblBacteria" id="AGZ42745">
        <property type="protein sequence ID" value="AGZ42745"/>
        <property type="gene ID" value="AFR_22375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4536"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666072" evidence="1">
        <location>
            <begin position="26"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="78"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="533" mass="56870" checksum="E7F97EACCCD57EFA" modified="2014-01-22" version="1">MHRRLALIATAALLLAGCSSTATDAADAGQPVSGGPLTWGIETEPITFNPHQYAQAKARLLVWNTFEGLLTHDTNGGFVPLLATGYEASPDGLTYTFKLRTGVTFSDGTPFDAAAVKANIDQLLAPGYAPAVAAVQLKNLESVEVADPATVKFHLARPDVLLLDFLSAPQGAVVSPKSLKSAANLKAGGPDLAGTGPFVLDRYAAGQEAHYKRNPSYWGERPAYLDEVTYRFLKESSVRVGALTSGQVQVIEGVPATDQELITGDPRLSLTRGLNSGSAYSYYLNVSRAPFDDLKVREAFRDAFDVDTALKAVYRGTATRAWSVISPTSPLYDKSLEGSYGNNPARANQLLDEAGWTQRDADGFRTKDGKRLTVRLVQNAPFVRDRRDILAQTVQAAVKQSAGIDLKVSLVDQGTATKALEDNEYEVFDNSRADTDAGAALGLLLHSAGAINRTGAKDPALDRLLDEGQATADAGKRLATYRAIQQQVVADQAVVLPLYAPSDQIAAARTVGGLSFEPTAGVPNSAYNVWISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W472</accession>
    <name>U5W472_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_28760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560350.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28760"/>
    <dbReference type="EnsemblBacteria" id="AGZ44013">
        <property type="protein sequence ID" value="AGZ44013"/>
        <property type="gene ID" value="AFR_28760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5830"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_11"/>
    <dbReference type="OrthoDB" id="955823at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="25"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="471" mass="50573" checksum="A52CDF8914ADEBAF" modified="2014-01-22" version="1">MSQAQQDESGTQPQNDRLTGSIVVVALVSAISGLLYGYDTGIISGALLQIGDDFKIGHVWEQVIAASILVGAVIGALTCSRLAERRGRHGTLLLIGIIFVVGAVAASISPDPITLSLSRLVLGFAVGGATQTAPVYVAELAPTAHRGRLVLFFQIAIGVGIVIATIVGATEVIDWRVSIGLAAVPAAIMFALMLRLPESPRWLAREGHEDRAREALARVRHAGSDLTAEFREIQENVRDEKRADTRGWAGLKAAWVRPALVVGCGLAIFTQLSGIEMIVYYSPTILTDNGFSDSTALRVSVALGLTYLIAQLIGLSIIDRVGRRRLTLVTLPFAALALFVLGWFFVSGNDGKSMVPWIIATLITFMALTAGGIQLMGWLTGSEIYPLATRAAGAAAQSASLWGTNVLITLSLLSIMRLIGTGQTFWLYAVFNLIAFVFLYRKMPELTGRSLEQIEDHLARKKFTPADFASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W486</accession>
    <name>U5W486_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-lactate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22280"/>
    <dbReference type="EnsemblBacteria" id="AGZ42726">
        <property type="protein sequence ID" value="AGZ42726"/>
        <property type="gene ID" value="AFR_22280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4516"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_9_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-linked_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="SSF55103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="35"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="441" mass="45442" checksum="2CDA9C0084832DA0" modified="2014-01-22" version="1">MTTDPRQWLPAAGVDWTDEADVVVSRSIDRSGWPPSGVPAAVVFPRDAADVRRTLRLANDARTPVVPRGAGTSLTGASTAGTGTVVLDLGRMNRIVALSPEDATVVVEPGVITADVDRAAAGHGLRYAPDPASHEISTIGGNIATNAGGLRCAKYGVTRDSVLGLDVVLADGRQISTGATTLKSVSGYDLTGLFVGSEGTLGVVVRATLRLRPAPLATTTIAASFADIATAAAASVAVTGARVQPVIAELLDEATLTAIDAAQGTSLRSRGAALLILQTEGAGEADLVAAVLREPAVAVEVSTDGTDLLAARRLALPSIERLGRPLIEDIAVPRSRLADAVLQIAAIAERHDVPICTLAHAGDGNLHPIIVTADDDLARRTAGDIFELALKLGGTLTGEHGIGTLKRPWLGRELGPAAHALQRQLKDLFDPYGILNPGKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W491</accession>
    <name>U5W491_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aliphatic sulfonates family ABC transporter, periplasmic ligand-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_22305</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein SsuA/TauA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363102.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22305"/>
    <dbReference type="EnsemblBacteria" id="AGZ42731">
        <property type="protein sequence ID" value="AGZ42731"/>
        <property type="gene ID" value="AFR_22305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4522"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_2_0_11"/>
    <dbReference type="OrthoDB" id="785019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010067">
        <property type="entry name" value="ABC_SsuA_sub-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01728">
        <property type="entry name" value="SsuA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666075" evidence="4">
        <location>
            <begin position="27"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="5">
        <location>
            <begin position="35"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="315" mass="33378" checksum="5D8CD90FF83ABC23" modified="2014-01-22" version="1">MHRRRAISALLALTVSVALGGCGDDADSAGGDSGPLRVGYQRFGGLSLVKAKAAAPGVQWSLFESGPALTEALKAGAIDIGQTGEAPPIFAAAAKIPFSIVGTSEPVPKGEAVLVKQAKGYRTFADLRGKTVALNKGSNVHWLLVKLLEANNMTLADINVKYLKPAEGRPAFDNDQVDAWIIWDPYFALAEQPGVQVLADATGLANNREYLLVHPDAATSKGAQIRTFLDAYQETTTWGIANPEERAKILAPELKIDQAVTARALARSAQPLAPLTPEIGAELQGIADGFTKLELIPGSVDMKARIDDQFTAELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4H4</accession>
    <name>U5W4H4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galactosylceramidase</fullName>
            <ecNumber evidence="2">3.2.1.46</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Galactosylceramidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_29160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 59 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.46" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="U5W4H4"/>
    <dbReference type="STRING" id="1246995.AFR_29160"/>
    <dbReference type="EnsemblBacteria" id="AGZ44093">
        <property type="protein sequence ID" value="AGZ44093"/>
        <property type="gene ID" value="AFR_29160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5907"/>
    <dbReference type="eggNOG" id="COG5520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015456_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004336">
        <property type="term" value="F:galactosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006683">
        <property type="term" value="P:galactosylceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001286">
        <property type="entry name" value="Glyco_hydro_59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15172">
        <property type="entry name" value="PTHR15172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02057">
        <property type="entry name" value="Glyco_hydro_59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00850">
        <property type="entry name" value="GLHYDRLASE59"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0746">Sphingolipid metabolism</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666081" description="Galactosylceramidase" evidence="6">
        <location>
            <begin position="37"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="7">
        <location>
            <begin position="672"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="797" mass="83299" checksum="99CFC1789B0A355F" modified="2014-01-22" version="1">MDPQMTRPPRTLAVLAVSSLLAALGVVAGFAAPAQAATAITVNGASGGRTFDGVGAVSGGGGNSRLLIDYPEPQRSQILDYLFRPGYGAALQTLKVEIGGDTNSTSGAEPSHAHFRGDLDCNRGYQWWIMEEAKRRNPAIQLIGLPWGAPGWIGNGTFLSGDLSGYLLSWLGCARQHNLTIDYLVPAQNERQWNAGTTIAVRNALDANGYGNVKIEVAEDWTYSPADQFAANPALRDAVDAIGVHYSCGYLTDQKTCTSSATAINSGKALWSSENGSQDYNGGAKPLARGINRTYLDAKMTGYQNWDLVASTTPNNLWPTVGLVLANQPWSGFYSVGKDVWSIAHTTQFTEPGWKYLDSSSGYIAGNRNNGSYITMRSPDSADYSMIIEAMDASGAQTLDLSVTGGLSTGAVHVWASNLNSSNPADHFTRQADITPSGGRYSLTVQPGYQYTITTTTGQGKGTATGPGQGTLALPHTDNFDSYPAGREAKYLMDMQGAFETVACGGGRTGMCVRQASTQAPITWKALSDPYAELGSTTWTNYTVQADVMLERSGYAELLGRVGGQELMNPGALNAYHLRVSDTGAWSVLRTNTSAQTTTLRSGSTAALGTGRWHSVALGFSGSTITATVDGAVVGTVADSTLGSGLAGIGTSQGQTAQFDNLNIAAGAGGGTSPVLRNVNANRCLDVPNASQANGTQLALWDCNAGANQQWTATAAGQLQVYGTKCLDAGAGTSGTRAVIAACTGAAGQQWRLNADDSVTSAQSGLCLDASGAGTANGTAVILWTCTNGTNQKWRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4K1</accession>
    <name>U5W4K1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23275"/>
    <dbReference type="EnsemblBacteria" id="AGZ42925">
        <property type="protein sequence ID" value="AGZ42925"/>
        <property type="gene ID" value="AFR_23275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4717"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_7_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019921">
        <property type="entry name" value="Lucif-like_OxRdtase_Rv2161c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03619">
        <property type="entry name" value="F420_Rv2161c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="32325" checksum="9BD0516B32EB9A34" modified="2014-01-22" version="1">MALPTSGSLASVPNIRRVAQEAERLEYAALWTYERLLRPMAKMVPGADGALERIPAEYRETYEPLETLSYVAALTDRIRLGTSIIDALFHVPVVLARRFATLDQLSGGRVIAGIGQGWMPQEFATANVPLKRRGAGFDEMVAAMRACWGPDPVEYQGRFYTIEASEVNPKPVQQHLPIIVGANTEAGQQRAARIADGINPNAFSYEQLVASVQTFRTAAADNGRDASTLTVTARANVPITADPIGEGRPYLGGSPVEIAGDLARLAEAGLDHVLFYNVAPDGIESHLRLMSELRERVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4U1</accession>
    <name>U5W4U1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29785"/>
    <dbReference type="EnsemblBacteria" id="AGZ44218">
        <property type="protein sequence ID" value="AGZ44218"/>
        <property type="gene ID" value="AFR_29785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6035"/>
    <dbReference type="eggNOG" id="COG1219">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2406681_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="92" mass="10109" checksum="5878647D7C681536" modified="2014-01-22" version="1">MHQELLWDQRALAAADVLTDERNAEAGVALPVAGLYPSLHLNLADCYRKVGDLEHAREHLQHARAGIGALGDDEYGQLIRDGLDRLAEQVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4V8</accession>
    <name>U5W4V8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29465"/>
    <dbReference type="EnsemblBacteria" id="AGZ44154">
        <property type="protein sequence ID" value="AGZ44154"/>
        <property type="gene ID" value="AFR_29465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5972"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_2_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="15018" checksum="904DF6B4432B92FF" modified="2014-01-22" version="1">MARAVLVVPKALDADLIAGCGLNLAEYIVLVNLSEAPHRTLRMYELAAESTLTSGGVTRLVARMERQGLVTRGQAEHDGRGMTATLTAAGLQRLQQAYPAALASVRTNVMDHLADLDLESFAAAVADFGQNRHQAAEGPPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W577</accession>
    <name>U5W577_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phytase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30565"/>
    <dbReference type="EnsemblBacteria" id="AGZ44373">
        <property type="protein sequence ID" value="AGZ44373"/>
        <property type="gene ID" value="AFR_30565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6188"/>
    <dbReference type="eggNOG" id="COG4222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048268_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027372">
        <property type="entry name" value="Phytase-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13449">
        <property type="entry name" value="Phytase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phytase-like" evidence="1">
        <location>
            <begin position="58"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="380" mass="40465" checksum="A2AB065772228CF9" modified="2014-01-22" version="1">MAALGLIATPASAHLATTPTLTGFASLPALTFVPGSEPSGAALGTAPVNGIAAPWDDQPVQGFSGVLPNGDGTYDVLSDNGFGNKANSADFLLRIHRIGPVPQTGKVDVLGGLNLTDPDAKVPFPLTRTDRVLTGSDFDVESIVRDRDGSLWIGDEFGPWLLHFDRAGRLLTAPIPLPDVRAPENPAGTPNLGGSKGFEGMAHSPDGRYLYPLLEGTVAGDTPGDLRFNEFDVRRAAYTGKRWTYRLDSPSNAIGDAIPVDANRFLIIERDNNQGDAAALKRIYLADRRDRNHDGVMDKTLVVDLLAIANPRGVGGFGKVFRFPFVTIEDVALLDGRTIAVLNDNNFPGSAGRTPGVADNNEFITVKLPKSLNPDPRVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5J3</accession>
    <name>U5W5J3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dala_Dala_lig_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363845.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24945"/>
    <dbReference type="EnsemblBacteria" id="AGZ43255">
        <property type="protein sequence ID" value="AGZ43255"/>
        <property type="gene ID" value="AFR_24945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5053"/>
    <dbReference type="eggNOG" id="COG1181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663616_0_0_11"/>
    <dbReference type="OrthoDB" id="407111at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dala_Dala_lig_C" evidence="1">
        <location>
            <begin position="144"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="411" mass="47625" checksum="72FDAA3F34B44CD4" modified="2014-01-22" version="1">MADVEHTIGLLLGNEDDWPRAYEALLRRVGVITDSQGRSHRTRSVRVTIEPFNLRDKPKHDLVIDRLAYWYYHPREWLKKIALMDDVYLLNSPFTFQSMEKHAAYCAMMRLGLKVPETVLVPFKNPLDNSRYAYTSQRYNQSFDLEALAESVGYPMYMKPYDGGAWVGVSKIRNVDELHAAYDASGERLMHLQAAVEDYDVFARSLTIGPETMVMKFRPELPMHERYAVDHNFLSPEAGDEVVTISRLVNAFFRWEFNSCESLVRGTEVHPIDYANACPDVAVTSLHYYFPWAMSALLRWTIYCVVTGRKPRLDTDTAAYFAIGDDPSLSYQEKLAGYRKLADDYFEIEQYEEFCAKHLGHVDEMVADWVGSEEFRSLLRETVRAMYPPHEHERFLGHFNGLIDAWLRDHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5P4</accession>
    <name>U5W5P4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24900"/>
    <dbReference type="EnsemblBacteria" id="AGZ43246">
        <property type="protein sequence ID" value="AGZ43246"/>
        <property type="gene ID" value="AFR_24900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5044"/>
    <dbReference type="eggNOG" id="COG3568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116745_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="SSF74853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666370" description="LTD domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="LTD" evidence="2">
        <location>
            <begin position="22"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="15223" checksum="6EB3B724E3A126FB" modified="2014-01-22" version="1">MAATAAVAVGGVTMIALPADAATLPFQITGVYYNSPGSDTRSNASLNAEYVKITSKFGRTVDLKGYTLRDNSSHVYTFGSVKVKGHESVWVRTGKGTNTPYTLFWGSGAYIWNNTGDKATLKDAAGKVMDTCSWTNKGSFTSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W649</accession>
    <name>U5W649_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364117.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25810"/>
    <dbReference type="EnsemblBacteria" id="AGZ43426">
        <property type="protein sequence ID" value="AGZ43426"/>
        <property type="gene ID" value="AFR_25810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5232"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118025_0_0_11"/>
    <dbReference type="OrthoDB" id="1983082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="160" mass="17524" checksum="D9EF8C232315A724" modified="2014-01-22" version="1">MSVVIRALRGGDVGAVVEFSLRAWAPVFESFNKVLGEDIYQRVYPDWLASQARDVAQVCRDHAGTTWVAEDDGAPVGFVAVVVSDDRRSADIEMIAVDPRWQRLGVSRALMDFALEQMRAAGVRLVGVVTGGDPGHEPARRAYESAGFTALPLVRYYKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6A7</accession>
    <name>U5W6A7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560992.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31995"/>
    <dbReference type="EnsemblBacteria" id="AGZ44659">
        <property type="protein sequence ID" value="AGZ44659"/>
        <property type="gene ID" value="AFR_31995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6476"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132093_0_0_11"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="37"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="12764" checksum="CDA01D3B97975329" modified="2014-01-22" version="1">MSVIREHQAVAHQLHGATFHSFVAPSSGSAELCAWRLDIAAGTVGVPHRVSREEILLIMSGEIMATVDGAATTVRPGEVIFVPAGASFGVDNTSGGPASAWVTTTVGLEATLADGSSISPPWVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6K5</accession>
    <name>U5W6K5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31885"/>
    <dbReference type="EnsemblBacteria" id="AGZ44637">
        <property type="protein sequence ID" value="AGZ44637"/>
        <property type="gene ID" value="AFR_31885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6454"/>
    <dbReference type="eggNOG" id="COG3265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103816_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="154" mass="17117" checksum="FFA29F0ABA2BAC4B" modified="2014-01-22" version="1">MGNEIHEQLSEARIHHCLIEGDNLDLAYPPPWEHNLGARNLGAMWANYRELGYRRMVYTNTVSVRFVDELTAAMGDDPRVVAVLLTASDATARVRLARREIGSALERHVERSDVAARELDEFSPGWVHRVATDGRPVADIAAEVIGLAGWTSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6M4</accession>
    <name>U5W6M4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ZnF_CDGSH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_26820</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364506.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26820"/>
    <dbReference type="EnsemblBacteria" id="AGZ43625">
        <property type="protein sequence ID" value="AGZ43625"/>
        <property type="gene ID" value="AFR_26820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26820"/>
    <dbReference type="eggNOG" id="COG3369">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173940_0_2_11"/>
    <dbReference type="OrthoDB" id="2002590at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043231">
        <property type="term" value="C:intracellular membrane-bounded organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018967">
        <property type="entry name" value="FeS-contain_CDGSH-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09360">
        <property type="entry name" value="zf-CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00704">
        <property type="entry name" value="ZnF_CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZnF_CDGSH" evidence="4">
        <location>
            <begin position="26"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="93" mass="9924" checksum="BCE36E32D5404E1E" modified="2014-01-22" version="1">MNDEHAATVIPYEDGPLLLRGDFTIRTPEGEVIEPGRGTVALCRCGKSAIKPFCDGTHKAIGFRAGTARETPAPRDQGVQRSDDSPAAQRSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6S5</accession>
    <name>U5W6S5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-glutamate O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_26795</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364498.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26795"/>
    <dbReference type="EnsemblBacteria" id="AGZ43621">
        <property type="protein sequence ID" value="AGZ43621"/>
        <property type="gene ID" value="AFR_26795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5432"/>
    <dbReference type="eggNOG" id="COG1352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000892_4_0_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="361"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="495"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="408"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ43621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="615" mass="68888" checksum="10F5CE173708F5E8" modified="2014-01-22" version="1">MISTAPDFEALLIHLKESRGFDFTGYKRSSLIRRVDRRMAQVGLSDYAEYQDFLQVHPDEFTALFNTILINVTGFFRDPDAWDYLRSDILKPILATRPGNAPIRVWSAGCASGEEAYTLAIVLAEALGVEEFRERVKIYATDVDEEQLTEARQAGYTEQQIAAVPAELVPKYFEQSAGKYLFRKDLRRSVIFGRNDLVQDAPISRIDLLTCRNTLMYFNAETQAKILTRFHFALSEGGILFLGRAEMLLSHGGIFTPVDLKRRVFRRVPRLHQQSGAVFAEALPTVQPPVGGLDELRNEAFAASPVAQLGLTSDGVVALANTRLEKLFGVSSRDIGRPFRDLDLSYRPVELRRYIEQAQIERRSMRVTDVEYLRGGELIHLEVQVSPLTGTDGSLLGVNLIFNDVTASRRLQDDLEHAHQQLEAAYEELQSTNEELETTNEELQSTVEELETTNEELQSTNEELETMNEELQSTNDELQTINDQLRISTAQLDDANAFLETVLTSLKAGVVVVDHNLRIRVWNDRAEDLWGLRSDEVVGQHFLNLDIGLPFDQLRPLLRGILTEAAVPAEVTVEAVNRRGRAVTVRVVCTPLTGPNTGPESGGAIIVMEAEQTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7F1</accession>
    <name>U5W7F1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">LexA repressor</fullName>
            <ecNumber evidence="12">3.4.21.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">lexA</name>
        <name evidence="16" type="ORF">AFR_34250</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolysis of Ala-|-Gly bond in repressor LexA.</text>
            <dbReference type="EC" id="3.4.21.88"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12 13">Belongs to the peptidase S24 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.88" evidence="12"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34250"/>
    <dbReference type="MEROPS" id="S24.001"/>
    <dbReference type="EnsemblBacteria" id="AGZ45108">
        <property type="protein sequence ID" value="AGZ45108"/>
        <property type="gene ID" value="AFR_34250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6930"/>
    <dbReference type="eggNOG" id="COG1974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_45_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06529">
        <property type="entry name" value="S24_LexA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00015">
        <property type="entry name" value="LexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006200">
        <property type="entry name" value="LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039418">
        <property type="entry name" value="LexA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006199">
        <property type="entry name" value="LexA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006197">
        <property type="entry name" value="Peptidase_S24_LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01726">
        <property type="entry name" value="LexA_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00726">
        <property type="entry name" value="LEXASERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00498">
        <property type="entry name" value="lexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="11 12" id="KW-0742">SOS response</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="LexA_DNA_bind" evidence="15">
        <location>
            <begin position="10"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S24" evidence="14">
        <location>
            <begin position="96"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="12">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="12">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="12">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="12">
        <location>
            <begin position="103"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AGZ45108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="23534" checksum="EF03767A3D2C6EE4" modified="2014-01-22" version="1">MHLAEDLDTSTLSPRQRAILTVIRDWVVRYGYSPSTREIADAVGLASTSSVSRHLRVLEERDFLRRGRSVTRPVDVRMFLEPVSRDDGDPTTVGVPLVGSVAAGSPILAEETLDEKLTLPRELVGRGTLFCLRVQGDSMVDAAICDGDIVVVRQQPDAYNGDIVCAMIDDEATVKVFRRRGGHVLLEPRNPAYEVIDGDRAVVLGKVVSVLRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Q3</accession>
    <name>U5W7Q3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MerR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561486.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34455"/>
    <dbReference type="EnsemblBacteria" id="AGZ45149">
        <property type="protein sequence ID" value="AGZ45149"/>
        <property type="gene ID" value="AFR_34455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6971"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052806_1_0_11"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="30547" checksum="D5FA6B63D7C68359" modified="2014-01-22" version="1">MELLPIGELARRTGLTVKAIRFYADRGIVPPTGRSASGHRRYDTAAVARLELVRTLRELGLDLATIRRVVDREADLAEVAATHADALAVQIRTLSRRHAVLSAVARHKSTPEEMDLMHRLARLSAHERRHLIDTFLGTAFAGLPNFAGITRTLTPELPDDPEPAQLEAWVELAELVQDPGFAARLRLLAELYTDVRSPGLPRPDPVALVRDHDASVDAVLAHYPDREQLTSYLEAAADPRRERYLELLSVINGWPPPEPLTPVLTRFLSELRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7R6</accession>
    <name>U5W7R6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561506.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34565"/>
    <dbReference type="EnsemblBacteria" id="AGZ45169">
        <property type="protein sequence ID" value="AGZ45169"/>
        <property type="gene ID" value="AFR_34565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6992"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_17_1_11"/>
    <dbReference type="OrthoDB" id="1919220at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="380" mass="37604" checksum="F0C534B606681DFA" modified="2014-01-22" version="1">MRGFGVWLGAATVSLLGTQVLAFAMAWIAAGRGGTFAALVLTAINLPRVLLLLAGGAVADRFGAWRVMIAADVLMTVVMLGLVGAAARFGEQPVLLVTSALAVGVVDAFYLPASGAMPRLLVPGDGLPRALAARQLAGQAVAFAGPPLGGVVVAAAGLAAAALANAASFALVAVVLIALRRPVRPAARSPIMDGWRTAFADPVLRPALLLTAAAAAFLLPVSGLLVPLLARQRDWPATAGGAVAGTIALGTAVMVGAVLIWGAHRRPARAAVAGLLVAATGTALLALSPAAGAAVAAGFLTGLGTGLFSAHIAPLVLGTAPSTHLARVQAVLVLTQSVPLLITNNVLGGIADRVPVSTVLLACAAALATATVATRAREAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7W2</accession>
    <name>U5W7W2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28945"/>
    <dbReference type="EnsemblBacteria" id="AGZ44050">
        <property type="protein sequence ID" value="AGZ44050"/>
        <property type="gene ID" value="AFR_28945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5865"/>
    <dbReference type="HOGENOM" id="CLU_1782708_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031876">
        <property type="entry name" value="DUF4760"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15956">
        <property type="entry name" value="DUF4760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="15673" checksum="3B54D0F97841432C" modified="2014-01-22" version="1">METAQSGQLAQGTMEVVKLLTTPEMRSAAVVLRDMGEIPYGAWSEEQKSAVSLRCNNFDVAGMFVQSKMASEELIADGWTSSIQETYRIAGPHIAALRERFKNDTHWRYFEQLAIGSGAIGHTQPEVASAEPTSSGGAGITAPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W815</accession>
    <name>U5W815_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Heme peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561489.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34470"/>
    <dbReference type="EnsemblBacteria" id="AGZ45152">
        <property type="protein sequence ID" value="AGZ45152"/>
        <property type="gene ID" value="AFR_34470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6974"/>
    <dbReference type="eggNOG" id="COG2373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027852_0_0_11"/>
    <dbReference type="OrthoDB" id="40466at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="SSF48113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="556" mass="61301" checksum="DE7DA0B4EA5FF3F4" modified="2014-01-22" version="1">MRENYYVVGEGILTTDGKSGVCERPSTEASLRKFRFSRLVPKGGTISTTILEKIATAMTAAPSAEQPVQPESADPPVPAGYTYLGQFVDHDLTLDNTARALGEHVTVNELLQGRSPALDLDSLYGRGPGDRDDDRFYAPDKLKLRVGLTAAIDGPGTNVNLNGYDLPRVTQGSTKAERQRALIPDHRNDENLVVAQTHLAFIRFHNRIVDLLADQGVPGTILFDRAKELVVRHYQWMLRTDFLPRIVDKDIVEDVFTNGRKFFEVPAGGQANGQYRHIKSGDTPTMPIEFSVAAYRLGHSMVRAAYDWNRIFSGNRGTLPFLFIFSGTSGNFDPFSPNINDQESGLFERLPTNWAVDWRRMYDFAGETGRDDLAAPQGFNIAQRIDTQVVNPLAKMPEGTFGGNGTPPPAIQLNLAFRNLVRANMVELCSGQEAAKFLGFTPLTPEQVINDVVAGVLNPDEQTEFGNQTPLWFYILREAELNGGRLTGVGGRIVAEVFHRAIEGSRISTVQDPTWRPSLGPDPDTFRMVDLLLFAFENRGDLLAPLGDVAPTPPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8G2</accession>
    <name>U5W8G2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uridylate kinase</fullName>
            <shortName evidence="6">UK</shortName>
            <ecNumber evidence="6">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Uridine monophosphate kinase</fullName>
            <shortName evidence="6">UMP kinase</shortName>
            <shortName evidence="6">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pyrH</name>
        <name evidence="8" type="ORF">AFR_36210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561834.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36210"/>
    <dbReference type="EnsemblBacteria" id="AGZ45498">
        <property type="protein sequence ID" value="AGZ45498"/>
        <property type="gene ID" value="AFR_36210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7327"/>
    <dbReference type="eggNOG" id="COG0528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033861_0_0_11"/>
    <dbReference type="OrthoDB" id="1043239at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02075">
        <property type="entry name" value="pyrH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="7">
        <location>
            <begin position="24"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="150"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="254" mass="27266" checksum="3FD5DDC9340BB199" modified="2014-01-22" version="1">MTMVTEQAVVVEEVPAHASERPRRVVLKLSGEVFGGGAVGVDPDVVQGIARQIATATRRGVQIAVVVGGGNFFRGAELQKRGMDRNRADYMGMLGTVMNCLALQDFLEKEGIETRVQTAITMAQVAEPYIPLRAIRHLEKGRVVIFGAGAGMPYFSTDTVTAQRALEIHADLVLMSKNGVDGVYTADPRTDPNARKIDHITFASVLQQGLRVADQAAFSLCEENGLPMLVFGAQGDDTIVRAVSGEKIGTLITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8M9</accession>
    <name>U5W8M9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6 7">Elongation factor Ts</fullName>
            <shortName evidence="6">EF-Ts</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">tsf</name>
        <name evidence="10" type="ORF">AFR_36215</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6 7">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the EF-Ts family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561835.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36215"/>
    <dbReference type="EnsemblBacteria" id="AGZ45499">
        <property type="protein sequence ID" value="AGZ45499"/>
        <property type="gene ID" value="AFR_36215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7328"/>
    <dbReference type="eggNOG" id="COG0264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047155_0_0_11"/>
    <dbReference type="OrthoDB" id="1405357at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="PTHR11741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="SSF54713"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00116">
        <property type="entry name" value="tsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01127">
        <property type="entry name" value="EF_TS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF_TS" evidence="9">
        <location>
            <begin position="68"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in Mg(2+) ion dislocation from EF-Tu" evidence="6">
        <location>
            <begin position="76"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="29929" checksum="E987398E787DF684" modified="2014-01-22" version="1">MANYTAADVKKIRDLTGAGMMDCKKALDESDGDFDKAVEFLRIKGAKDVGKRAGRTAANGIVSHSGKALLELNCETDFVAKTPDFVALGQSLVELGEQNKLADAAALQAASFADGRTVADVVQDFSAKIGEKIVVNRFALLDGTVAVYLHRKAQDLPPQVGVLVEYTGKSDEAGDDDARAVGMQIAAMRPKFLTREEVPAETVESERRIAEETAREEGKPEQALSKIVEGRVNSFFKDYVLLEQPSVTDNKKTVKQVTADSGIQVTRFVRFEVGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8N7</accession>
    <name>U5W8N7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36265"/>
    <dbReference type="EnsemblBacteria" id="AGZ45509">
        <property type="protein sequence ID" value="AGZ45509"/>
        <property type="gene ID" value="AFR_36265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7339"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116212_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666205" evidence="1">
        <location>
            <begin position="30"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="15676" checksum="4B00670AAE97EBED" modified="2014-01-22" version="1">MVSARVLLTSVLALSACSGPSTSPDPPTASAGFGNAPATSAAFTSPSGNIGCYVDRDGARCDIVKKSWKPPAKPSDCDLAWGSSISLNSSSQQPMFTCAGDTVLGADKILNYGETIQVGDLRCDSTSQRTRCEDTGTGHGFTLAIEQYTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Y1</accession>
    <name>U5W8Y1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561813.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36105"/>
    <dbReference type="EnsemblBacteria" id="AGZ45477">
        <property type="protein sequence ID" value="AGZ45477"/>
        <property type="gene ID" value="AFR_36105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7307"/>
    <dbReference type="eggNOG" id="COG1668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046841_3_1_11"/>
    <dbReference type="OrthoDB" id="1155696at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="355" mass="37474" checksum="4B25A8B522D82722" modified="2014-01-22" version="1">MTTFAAAKLVAARELRVRLKDKTFIFSTLFFILIAVGSTVLPSLLDGGPAKVAVTDRTVAAALQNADLNVRVVTSDAEAEKLVRDGDVEAAVVAGPKVLALEETPSEVVNALSSSPPVQLLDPDAVDPFLKFVVPFALAFLFFMTSFTFGLQIAMSVTEEKQTRIVEILVASVPVRALLAGKVAAMTLLAFGQIALISVVALAGMRIAGVGTDVIGLVGPVIGWFLPFFLIGFVMLATLWAGVGALAARQEDINGASAPLQMAVMGPFFAVLFLQDNVIAMKILSYIPFSAPIAMPVRLFNGDAAGWEPFVALAVLIVATVLFLGVGARVYEGSLLRTNGRTSFATAWRSRSAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Z1</accession>
    <name>U5W8Z1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561925.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36665"/>
    <dbReference type="EnsemblBacteria" id="AGZ45589">
        <property type="protein sequence ID" value="AGZ45589"/>
        <property type="gene ID" value="AFR_36665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7417"/>
    <dbReference type="eggNOG" id="COG1920">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_203151_0_0_11"/>
    <dbReference type="OrthoDB" id="2065806at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="65" mass="6869" checksum="7DD5DAE937A0FD08" modified="2014-01-22" version="1">MQGTVATFDPQSRSGTLLLDDGSELAFPAEAFQASGLRLLRLGQRLSIESGPDGNVIKVQLPGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W911</accession>
    <name>U5W911_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0102 protein AFR_36230</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0102 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36230"/>
    <dbReference type="EnsemblBacteria" id="AGZ45502">
        <property type="protein sequence ID" value="AGZ45502"/>
        <property type="gene ID" value="AFR_36230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7331"/>
    <dbReference type="eggNOG" id="COG0792">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115353_2_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00048">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003509">
        <property type="entry name" value="UPF0102"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34039">
        <property type="entry name" value="PTHR34039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02021">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="105" mass="11876" checksum="926536A607213501" modified="2014-01-22" version="1">MAEEHLRAQGLVVLARNWRCADGEIDLILRDGDDVVFCEVKTRRGDRFGTPAEAIGPAKVRRLRRLAAHWLSESPVRPHEIRFDVVAVLPQRRGATVVEHVRAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W963</accession>
    <name>U5W963_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_31105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560815.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31105"/>
    <dbReference type="EnsemblBacteria" id="AGZ44481">
        <property type="protein sequence ID" value="AGZ44481"/>
        <property type="gene ID" value="AFR_31105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6296"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_1_11"/>
    <dbReference type="OrthoDB" id="948440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="3">
        <location>
            <begin position="15"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="384" mass="40677" checksum="39CBAC404F7BF658" modified="2014-01-22" version="1">MRIGIVSPYSFDVPGGVQNHIVDLAEALIGLGHEVSVLAPADEDSELPPYVVAAGRAVPLPYNGSVARIAFGPISTARVRRWLARGKFDVLHVHEPLTLSLSLLAVLSARGPVVATFHTAMIRSRALSAAGGLLQLVVEKITARIAVSELARKVQVEHLGGGAVEIPNGVAVAKFSHAEALPGRPGEGGTLGFLGRFTESRKGFELLRTAFVTLARERPGLRLLVAGPGDRSDLYDDIPKELHDRVEFLGLVSEADKARMLRSVDIYVAPNTGGESFGMILTEAMAAGAAIAASDLDAFRRVLDGGRAGALFPTGDAGALTGLLGELLDDPRRRAELAACARVAVQAFDWPSVAKRVLEVYATAIEATDGRVFDEEWAEPRSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9S7</accession>
    <name>U5W9S7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hepatocyte growth factor receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44730">
        <property type="protein sequence ID" value="AGZ44730"/>
        <property type="gene ID" value="AFR_32360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6549"/>
    <dbReference type="eggNOG" id="ENOG502ZD2M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1575131_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="17911" checksum="275263FC7E69BA28" modified="2014-01-22" version="1">MTPTPGDPDDPIYFTYSNGIDVTPTTSPTDRNVDLDIMGVGFLGMDFDNTGATLANADTAHVYLVRGEYDPTDASNLKANGPVVECRSVLVISDTEIICTINLATKLDPADQTTPMTGPNDEVPLGAYTVTVVNNGDIDVQPSGANEDLTNPFVKTDLTSTSTFTVAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9T6</accession>
    <name>U5W9T6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561075.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44740">
        <property type="protein sequence ID" value="AGZ44740"/>
        <property type="gene ID" value="AFR_32410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6559"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_2_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="32545" checksum="6A9C2A7C97367289" modified="2014-01-22" version="1">MKWTEQQIPRQDGRVAVVTGANTGLGFETARQLAEHGASVVLAVRDTAKGQQAAARINGDVSVRELDLSSLESVRAAAAGLRADHPKIDLLINNAGVMYTPRQTTREGFELQFGTNHLGHFAFTGLLLDLLLPVPGSRVVTVSSTGHRIRAAIHFDDLQWERSYSRIGAYGQSKLANLMFTYELQRRLAAHGTTVAVAAHPGISNTELVRNTPALVRRPVAWLAPMLTQSAAAGALPTLRAAADPSVLGGQYYGPDGLNEARGHPKPATSSADSYDLTLQQRLWAVSEDLTGVRFPVGRVAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAT5</accession>
    <name>U5WAT5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_40025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40025"/>
    <dbReference type="EnsemblBacteria" id="AGZ46259">
        <property type="protein sequence ID" value="AGZ46259"/>
        <property type="gene ID" value="AFR_40025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8100"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035158544" evidence="2">
        <location>
            <begin position="25"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="10"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="146"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="30828" checksum="C9977F6C5EA4713A" modified="2014-01-22" version="1">MLTGAALTVAVLAVSSSAPLIAFAAAPALAVAFWRNGLAFAALTPITVGPRRFELATLRSRSGVFAVLAGVALALHFATWMPSVQLGSVATATALVATQPVWQGLIAAAQGKRPSTAGWVGIGLAVAGAAWATGIDIGVSGQAVLADVLALIGAIAASIYTAYGERARASLSTTTYTWVCYGTCGALLLVVCVVGRVQLHGYDNRTWAAILALVVGAQLLGHSMFNYALHRTSATTVSVLILLEVPGAALLAWAWLGQTPRVAALPGLGLLVVGVAVVVLGGARRRAALGSPAAPADVADPAPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAW5</accession>
    <name>U5WAW5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34040"/>
    <dbReference type="EnsemblBacteria" id="AGZ45066">
        <property type="protein sequence ID" value="AGZ45066"/>
        <property type="gene ID" value="AFR_34040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34040"/>
    <dbReference type="eggNOG" id="COG1357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029034_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="319" mass="35524" checksum="D057328B0D53CFB9" modified="2014-01-22" version="1">MGLANQAIPASRPQAQIDAVKTGLSVAAGIAAAGALMLAVRRQRLAEIAHLSQDHDAAEKRKNEMYVKAVEQIASERPQVRMAGFYALEQLGQNNPEVRQMVVDVICAYLRMPFVPPPEAYRIPYWARDAANDLALMSLEGRLDEAHQARFEEWQVRMLAQEILDHKTLKYGDPVPDSYWPGLSLNLTDAVLVNWHFGGGHIEHGNFSGATFIGRCFLDNTTFTGQAWFRGAVFVGPTSFFRSVFEFTADFHDAEFKQDAHFVEAEFARKPDFKGTIFAKTPVALRAKLADVVVEDGIFEKNAADARHMISNDSKRPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBC8</accession>
    <name>U5WBC8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative type-III polyketide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_41295</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41295"/>
    <dbReference type="EnsemblBacteria" id="AGZ46513">
        <property type="protein sequence ID" value="AGZ46513"/>
        <property type="gene ID" value="AFR_41295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8357"/>
    <dbReference type="eggNOG" id="COG3424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034992_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="PTHR11877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000451">
        <property type="entry name" value="PKS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chal_sti_synt_N" evidence="3">
        <location>
            <begin position="3"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Chal_sti_synt_C" evidence="4">
        <location>
            <begin position="225"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000451-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="353" mass="37334" checksum="95E4D3C078E28C89" modified="2014-01-22" version="1">MKNIGQAVIAGMGTALPPSAAQDDLWKGFFSDHYAGGRRGLAQRIFANSGVRTRQAAVSPLLEDVSGWSTERRMRRYQVEAMPLAKEAVGRALTEAGVAADELGLFVVCSCTGYATPGLDILLARDMGMAPSLQRLFVGHMGCYAALPGLGTAADFVAARGRPALLLCAELTSLHLQPTDPRADIQQIVSHALFSDAAAAVVLTPSSTPGGYAVSDVSALTDTTTADHMTWDVTDLGFRMGLSPQVPTVLSVHVRPMVDDLLTRNGLTIGDVDGWAVHPGGPKILDVVEEQLGLATDAMAPSREVLSSYGNCSSPTVLLVLDALRRRADPPRRVVMLAFGPGLTLYGVLLERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBF5</accession>
    <name>U5WBF5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561612.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35100"/>
    <dbReference type="EnsemblBacteria" id="AGZ45276">
        <property type="protein sequence ID" value="AGZ45276"/>
        <property type="gene ID" value="AFR_35100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7102"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_11"/>
    <dbReference type="OrthoDB" id="1752728at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="10"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="332" mass="35303" checksum="DDB00322C7C45DF9" modified="2014-01-22" version="1">MKAIQFHEAGGPEVLQYEEVPVPELGPGEVLVRVHAAGINPPDWYLREGLKVMPAEMRPPLEFPLTPGTDMSGVVEAVAPDVAEFAVGDEVFGMLRFPGFDGRTYAEHVAAPASDLAHKPAGLDPVQAAGAPMAVLTAWQYLIDLGHDVPSPFTGQVHQPVPITPEMTVLVNGAAGGVGHFAVQLAKWKGAHVIAVASGRHEQFLRKLGADQFIDYTTTRAADVVSGVDLVIDTVGGPDSSRFLTVLKRGGTMLPVFFAQYDPEETERLGISVSNIQVRSNGPQLAEIGRLFDEGKLQTGVDSTYPLSEAAAAHTRAAQGHIQGKIVLTVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBG4</accession>
    <name>U5WBG4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561661.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35345"/>
    <dbReference type="EnsemblBacteria" id="AGZ45325">
        <property type="protein sequence ID" value="AGZ45325"/>
        <property type="gene ID" value="AFR_35345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7152"/>
    <dbReference type="eggNOG" id="ENOG50331UF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142113_3_0_11"/>
    <dbReference type="OrthoDB" id="1912439at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021400">
        <property type="entry name" value="DUF3039"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11238">
        <property type="entry name" value="DUF3039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="72" mass="8089" checksum="5C6FEA989B1786EE" modified="2014-01-22" version="1">MSTQILERPETKDADTGPEMFHYVRKEKIAESAVMGTYVIALCGEKFPVTKTPKPGSPVCPQCKEIYESMKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBG6</accession>
    <name>U5WBG6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_41175</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562821.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41175"/>
    <dbReference type="EnsemblBacteria" id="AGZ46489">
        <property type="protein sequence ID" value="AGZ46489"/>
        <property type="gene ID" value="AFR_41175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8334"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="25481" checksum="7C1593B2876DE9AB" modified="2014-01-22" version="1">MTAALRDVRMIYGPETRQVLALDDVTVEFPPGTFTAVMGPSGSGKSTLLHCAAGLERPTAGSVTIAGTDLGGLTETALTHLRRERVGFVFQAFNLVSSLTAAQNVALPLRLAGRRPARQEISAALAAVGLADRARHRPSELSGGQQQRVAIARALITRPAVVFADEPTGALDNRSSKQVLDLLRALVDDHGQTVVMVTHDPVAAAFADRVLMLADGRLVDELEDADAASIAARMTALELAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBG8</accession>
    <name>U5WBG8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ENDO3c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35370"/>
    <dbReference type="EnsemblBacteria" id="AGZ45330">
        <property type="protein sequence ID" value="AGZ45330"/>
        <property type="gene ID" value="AFR_35370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7157"/>
    <dbReference type="eggNOG" id="COG0177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117222_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017658">
        <property type="entry name" value="CHP03252_HhH-GPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03252">
        <property type="entry name" value="TIGR03252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="1">
        <location>
            <begin position="24"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20024" checksum="B414D757C0A820ED" modified="2014-01-22" version="1">MTLSLPIAAEANELLTNDPLALLIGMVLDQQSPLEKAFSSPYVLAQRLGHAPTAEELAGFDPEALVTIFATPPALHRFPKAMAARVQEVCRALVETYDGEAQNLWQGAGDGKELVKRIAALPGFGKQKSQIFAALLGKQYGVQPPGWRAAAGAYGEEGSHRSVADIVDGESLARVRAFKKEMKAAAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBR9</accession>
    <name>U5WBR9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0020 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561786.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35970"/>
    <dbReference type="EnsemblBacteria" id="AGZ45450">
        <property type="protein sequence ID" value="AGZ45450"/>
        <property type="gene ID" value="AFR_35970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7281"/>
    <dbReference type="eggNOG" id="COG1041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070253_0_0_11"/>
    <dbReference type="OrthoDB" id="1650598at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0020" evidence="1">
        <location>
            <begin position="139"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="341" mass="37364" checksum="4B6D8C8CFA053BC6" modified="2014-01-22" version="1">MSRYALLLAPSANRVYADQAGRLSRAELAAFRPVLSGPVSQVDAARIGGVEYLTFDADLQARDVAFLSNMSAAYALFERVDDDLLRPVELTPLARYDSDLITIPKYAGKTNEQFTKLLLNLTLLASASAKKMLDGGLIVGDPLAGRGTTLNQALMYGYDAIGVEIDGKDVEAYKLFLQTWLKRKRLKHSAEFNPVRRQGRRAARRMEITLAASKDDHKAGAVQKVTMLNADTTQLDGLLRGNCMDVIVTDLPYGIAHASYEDEGGMSRRPLDLLERAVPQWVTLLRPGGALGLSWNTKVAKRELAEDILVANGLSVVAEEELSHRVDQGIERDVVVARKPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBW9</accession>
    <name>U5WBW9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable dual-specificity RNA methyltransferase RlmN</fullName>
            <ecNumber evidence="11">2.1.1.192</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">23S rRNA (adenine(2503)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">23S rRNA m2A2503 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ribosomal RNA large subunit methyltransferase N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA (adenine(37)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA m2A37 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rlmN</name>
        <name evidence="13" type="ORF">AFR_36195</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(2503) in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42916"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10282"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43332"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10485"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the radical SAM superfamily. RlmN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.192" evidence="11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561831.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36195"/>
    <dbReference type="EnsemblBacteria" id="AGZ45495">
        <property type="protein sequence ID" value="AGZ45495"/>
        <property type="gene ID" value="AFR_36195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7324"/>
    <dbReference type="eggNOG" id="COG0820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029101_0_2_11"/>
    <dbReference type="OrthoDB" id="1111428at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070040">
        <property type="term" value="F:rRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002935">
        <property type="term" value="F:tRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01849">
        <property type="entry name" value="RNA_methyltr_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040072">
        <property type="entry name" value="Methyltransferase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027492">
        <property type="entry name" value="RNA_MTrfase_RlmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004383">
        <property type="entry name" value="rRNA_lsu_MTrfase_RlmN/Cfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544">
        <property type="entry name" value="PTHR30544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006004">
        <property type="entry name" value="CHP00048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00275">
        <property type="entry name" value="adenosine_C2_methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00048">
        <property type="entry name" value="rRNA_mod_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 11" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="123"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="11">
        <location>
            <begin position="192"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="11">
        <location>
            <begin position="249"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="active site" description="S-methylcysteine intermediate" evidence="11">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ45495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="392" mass="42062" checksum="06C8FCD782855999" modified="2014-01-22" version="1">MTILPVIPTSPDAPDAVITRRRPAMPPRHLADLDLAGRRAAVAELGEPAFRAQQLSTHYFGRLVRDPAAMTDLPAASRDRLTSDLLPTLLTPQREMACDDGATRKTLWKLHDNAMVESVLMGYPDRITACVSSQAGCGMACPFCATGQAGLTRNLSTAEIVDQVVYLAGVAASGAVTGSPPRLSRVVFMGMGEPLANYPRVIAAVRRLTSPAPEGLGLSQRHITVSTVGLVPAMRRLIAEELSVTLALSLHAPDDDLRDELVPVNQRWKVAEVLDAAFDYAAQTGRRISIEYALIRDVNDQPWRADLLGRLLHGKLAHVNLIPLNPTPGSKWDASPKPVEREFVRRLQGAGVSTTVRDTRGREIDGACGQLAASELTVVDAGGATADAEVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC03</accession>
    <name>U5WC03_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="3">PPIase</shortName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_42370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563059.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42370"/>
    <dbReference type="EnsemblBacteria" id="AGZ46728">
        <property type="protein sequence ID" value="AGZ46728"/>
        <property type="gene ID" value="AFR_42370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8575"/>
    <dbReference type="eggNOG" id="COG0652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_16_3_11"/>
    <dbReference type="OrthoDB" id="1861282at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="4">
        <location>
            <begin position="10"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="176" mass="18999" checksum="F7491ACEEF79DFA7" modified="2014-01-22" version="1">MADTLYATLHTNHGPIRLQLFPDHAPATVRNFVELAEGTKDYVDPRTGQKGNGPYYDGTISHRVIAGFMVQLGDPTGTGRGGPGFQFADEFHPELQFDRPYVLAMANAGPGTNGSQFFITVGPTPHLNRRHTIFGQVADEASAKVVDSIATTPTGPGDRPREDVVVERVEIERAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC26</accession>
    <name>U5WC26_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycerophosphoryl diester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563089.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42520"/>
    <dbReference type="EnsemblBacteria" id="AGZ46758">
        <property type="protein sequence ID" value="AGZ46758"/>
        <property type="gene ID" value="AFR_42520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8605"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_3_0_11"/>
    <dbReference type="OrthoDB" id="1542724at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="3"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="262" mass="28666" checksum="EC6C5284868AA6E6" modified="2014-01-22" version="1">MRPLVFAHRGGADALPEHTLAAYLRALDEGADGVECDVRLTRDGHLVCVHDRRLNRTSNGTGLVSAKTLAQLEALDFASWHPAAATEDLQVEHTRLLTLDRLLEALRERGRPVRLLIETKHPSRYGADVERRLVEMLRRYGLAEPVAGDPVTVTVMSFAALALRRIRTLAPALPTVYLLEILPPGVGRGRLPFGARIAGPGIGLVRARPNLIGNLRSAGLGAYCWTVNAEEDVDLVLKHGVDGIITDRPRLVLEHLEKVPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC96</accession>
    <name>U5WC96_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formate-tetrahydrofolate ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42970"/>
    <dbReference type="EnsemblBacteria" id="AGZ46848">
        <property type="protein sequence ID" value="AGZ46848"/>
        <property type="gene ID" value="AFR_42970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8695"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_8_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="25"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="283" mass="30432" checksum="574F0A784E25DEC6" modified="2014-01-22" version="1">MEALRAVQILNPSGEIIMPRPDHPRVLCVANQKGGVGKTTTTVNLAVALALHGNRVLVVDLDPQGNASTGLNVPHHAGVPDVYDCLIDNVPLADVAQPVEGIPNLFCVPATIDLAGAEIELVSVVARESRLQRAITAHPEKFDYVFIDCPPSLGLLTVNALCAAQEVLIPIQCEYYALEGLNQLINNINLVRQHLNPTLDVSTILLTMYDRRTRLADAVEQDVRNHFGSKVLEAVIPRNVRVSEAPSYGQSVMTYDPGSRGATSYFEAALEIAMRGVNTGGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDI9</accession>
    <name>U5WDI9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_38980</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562384.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38980"/>
    <dbReference type="EnsemblBacteria" id="AGZ46050">
        <property type="protein sequence ID" value="AGZ46050"/>
        <property type="gene ID" value="AFR_38980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7889"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_2_1_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003012">
        <property type="entry name" value="Tet_transcr_reg_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00400">
        <property type="entry name" value="TETREPRESSOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="6">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="22853" checksum="7C7A9F1BE8D660CF" modified="2014-01-22" version="1">MARPRHTLLSRERIVDAAAALVDAEGLDAVSVRRLATELGVAGPSLYNHFATKAEILDAVADAVVAQVDVSYFGTHDWSEALRLWAHSYHAVLSAHPNIVPVLAHGPGRRPAALAMADTVYGGLIAAGWPPARATHIGALMRYLVTGAALGSFALGFDEDPGLYAERYPHLRDAHLLAEHRRSVDEGAFALGLETVLAGLEVKKAGLDQLKGSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDL7</accession>
    <name>U5WDL7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39205"/>
    <dbReference type="EnsemblBacteria" id="AGZ46095">
        <property type="protein sequence ID" value="AGZ46095"/>
        <property type="gene ID" value="AFR_39205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7933"/>
    <dbReference type="HOGENOM" id="CLU_1583057_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="174" mass="18296" checksum="739C5710B8B8FB78" modified="2014-01-22" version="1">MGNVQSATEVEDDDEEAPGLWESFGAALRLIVGWFSIAIGILNLLVELDRGSAADGAYLLFHAMLLVGGVLMVSLAWIAARPGVAGWSAGGGVLAAGMLASGVPASDALCCLSAYAVRHGYPFSFAARDADGGRWHVDSQHLLADLLFWGYAGLIVLVVVALARRATHHHEARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDS9</accession>
    <name>U5WDS9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39060"/>
    <dbReference type="EnsemblBacteria" id="AGZ46066">
        <property type="protein sequence ID" value="AGZ46066"/>
        <property type="gene ID" value="AFR_39060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7905"/>
    <dbReference type="eggNOG" id="ENOG5033AIJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192240_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="64" mass="6412" checksum="92574D54F33F91C0" modified="2014-01-22" version="1">MTTDDLPAGIGAPATRALHGAGYHRLEDLNGASRAALAALHGVGPKALRLLDEALAARGLTLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE75</accession>
    <name>U5WE75_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562559.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39860"/>
    <dbReference type="EnsemblBacteria" id="AGZ46226">
        <property type="protein sequence ID" value="AGZ46226"/>
        <property type="gene ID" value="AFR_39860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8065"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_6_1_11"/>
    <dbReference type="OrthoDB" id="1857145at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="13244" checksum="093A8137C17BDCBA" modified="2014-01-22" version="1">MIDHVILGVHHLDESKHFYENALRPLGCKVVAEKPELIGFGDDSGRPFFWLAVREPSHRVHVAFTAPDRAAVDAFHAAALLAGGVDNGPPGLRPDYHENYYGAFAFDADGNNIEAVCHRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEA6</accession>
    <name>U5WEA6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562697.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40555"/>
    <dbReference type="EnsemblBacteria" id="AGZ46365">
        <property type="protein sequence ID" value="AGZ46365"/>
        <property type="gene ID" value="AFR_40555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8208"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138345_0_0_11"/>
    <dbReference type="OrthoDB" id="1645839at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17113" checksum="E769489A508825DC" modified="2014-01-22" version="1">MSDTSVIPVALTVRNLFEDLLGRDVNVSPGDPMTAADLSTGVIAIYTDTSQQLYAVLGMQLTLAANAGAALGLLPAGAAEDSIEEKKLFPNLAENVFELCNVLTSLLNREGAPHVKLYQVIYPGMDLPNDARAHLLALGKRLDLTVEVARYGKGKFSLSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEM7</accession>
    <name>U5WEM7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40735"/>
    <dbReference type="EnsemblBacteria" id="AGZ46401">
        <property type="protein sequence ID" value="AGZ46401"/>
        <property type="gene ID" value="AFR_40735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8246"/>
    <dbReference type="eggNOG" id="ENOG50335WC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036478_2_0_11"/>
    <dbReference type="OrthoDB" id="1327142at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021373">
        <property type="entry name" value="DUF2993"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11209">
        <property type="entry name" value="LmeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="262" mass="27347" checksum="013F3082C13A0859" modified="2014-01-22" version="1">MAEVYGSARPRKRWGRRLLITFVVLLLLILAVLVVADRMGASYAERVIGDRVTEQLTQQKATSEKPKVSIEGVPFLTQVLAGKYQEIKIGLDNFSGPAGNGKDIKMPLLDISAKDVDAPLDTVRTGNGDIVASTVTGAGTIGYANLAELTGQPGLKLSERGGKLVGAAPIEALGQTFNASGTANLEVKDGVVKVTFANVTADGLPDIPLVRSLVDTYVQKLAFDLKVPALPLDLKVQKVQPTAGGLVVTAGASNVSLSSGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEP2</accession>
    <name>U5WEP2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_40810</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562748.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40810"/>
    <dbReference type="EnsemblBacteria" id="AGZ46416">
        <property type="protein sequence ID" value="AGZ46416"/>
        <property type="gene ID" value="AFR_40810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8262"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_22_1_11"/>
    <dbReference type="OrthoDB" id="1335404at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="104"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="294" mass="31587" checksum="D0962C45FE964ED4" modified="2014-01-22" version="1">MPSDPFEAERPHLLAVAYRMLGSRAEAEDVVQEAWLRFNRAQAGGEIHDLRGWLTTVTGRLCLDVLKSARVRREAYPGQWLPEPVVSFLDDPAEKVTQRMDVSLALLVVLEKLTPEQRVAVVLHDAFAVPFDEVAAVLNTSVSAARQHASRGRRAVSEGEARHTADLAEQRKVLDAFLAAATSGDLNALAAVLAPEVVSIADGGGAVNAGRNAIFGLDKVVRLYAALFSRRSRDLESATTDAVLVNGDAGVLFRGRYRDGSPLLSVLSVAVADGRITALYNQLNPAKLGSLNDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFJ3</accession>
    <name>U5WFJ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46810">
        <property type="protein sequence ID" value="AGZ46810"/>
        <property type="gene ID" value="AFR_42780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42780"/>
    <dbReference type="HOGENOM" id="CLU_1891683_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="13921" checksum="08B9B89F581511A9" modified="2014-01-22" version="1">MNVGVEQGEDAVYAVDAAGGVGGRAAVVGQAEPFSRARIGQALHLAVRLSRHCLFQIQLMYGQRRPQAAAFAVRAGLVDRGWDVVAGQGRLVTGFDGAGRQGADVVVDADPVGRFDVDAFGSEFAGAYTVGLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNJ2</accession>
    <name>U5VNJ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_00325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357298.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00325"/>
    <dbReference type="EnsemblBacteria" id="AGZ38354">
        <property type="protein sequence ID" value="AGZ38354"/>
        <property type="gene ID" value="AFR_00325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.67"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005304_0_1_11"/>
    <dbReference type="OrthoDB" id="1951488at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036197">
        <property type="entry name" value="NarG-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103501">
        <property type="entry name" value="SSF103501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="289"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="377"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="713" mass="78233" checksum="582C49A390645912" modified="2014-01-22" version="1">MGTAQIIATVLAAAVTIVAVALAVRAVMQMTAVIRQGKPDPERFGDKGARTKTMLVETAGHTRMLKWGVVGAAHWFVMVAFVVLSLLVLEAYFEVVSPELGLPVIGHWVVFGLATEWIGVLGTIGILVLVAIRQRDKRKKVSRFTGSTMWQAYFVEAIIIAVLICGFLIRGFKVATDHFEYPFWATPVSHGIGALLPDWENGATWTALVKILISMSWLITISLNVTMGVAWHRFLAFFNIYFKRSPKKPAGSGMGALRPMMSQGKPLDFEEADPEKDQFGVAQVEQFTWKGLLDFSTCTECGRCQSQCPAWNTAKPLSPKLMILSLRDHAYAKAPYLLGGGGKDLTGEEKATEAQLAHMDVLALAEGNRPLIGTEEEQGVIDPDVLWSCTTCGACVEQCPVDIEHIDHIVDMRRYQVLIESSFPSEAGVMLRNLENKGNPWGAPQNTREDWTKGLDFEIKRVGEEEFDYLFWVGCAGAFEDRAKKTTRAVATLLHEAGVDFAILGEGETCTGDPARRIGNEFVFQMLAQQNVETLQEAKVKRIVATCPHCFNTLGNEYEQLGLKVEVVHHTQLLAHLVAEGKLTPVQPVDGGVTYHDPCYLGRHNRVFEAPREVLGSAIEGGLTEMPRNSERSFCCGAGGARMWMEEKIGKRINVERTEEALATGAKTIAVGCPFCYTMIGDGVTGKGEQENVEVIDVASVLLRSIKGDKAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP21</accession>
    <name>U5VP21_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DJ-1_PfpI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357641.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02045"/>
    <dbReference type="MEROPS" id="C56.001"/>
    <dbReference type="EnsemblBacteria" id="AGZ38698">
        <property type="protein sequence ID" value="AGZ38698"/>
        <property type="gene ID" value="AFR_02045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.414"/>
    <dbReference type="eggNOG" id="COG0693">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_44_4_11"/>
    <dbReference type="OrthoDB" id="1421548at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006286">
        <property type="entry name" value="C56_PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733">
        <property type="entry name" value="PTHR42733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01382">
        <property type="entry name" value="PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51276">
        <property type="entry name" value="PEPTIDASE_C56_PFPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="2">
        <location>
            <begin position="8"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="179" mass="19368" checksum="73BECA2688DD8542" modified="2014-01-22" version="1">MATSIKGKRVAFLATDGVEEVEYTEPRKAVESAGGSAELVSIKDGDIQAVNHLDKAGTYPVERQVKDAVVDDYDALVLPGGVANPDFLRMDLDAVRFVRDFFSSGKPVAAICHGPWTLVEAGVVDGRTLTSWPSLRTDLANAGATWVDEQVYVDNGLVTSRKPDDIPAFCDKLLEELSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP35</accession>
    <name>U5VP35_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357482.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01250"/>
    <dbReference type="EnsemblBacteria" id="AGZ38539">
        <property type="protein sequence ID" value="AGZ38539"/>
        <property type="gene ID" value="AFR_01250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.249"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_529605_0_0_11"/>
    <dbReference type="OrthoDB" id="1863545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="506" mass="52338" checksum="C0D6290403C49CE1" modified="2014-01-22" version="1">MKPSTLGRLAVAGGRTDVLRMSLTAGSSVLAAVVLLAAATVVAVPELGTSDQGSEAWNDYASPLIAEPGLRPGVVITLLLLAVPVLFLAGQSIRFGSPARDRRLAAMRLAGATPGQAVLIAATETAVASLLGSIVGTAVFLVLQQSLNARNGDGLLYLPSDQLPSVVSFLLVLLLVPVLAGLIGAFLLRRVIITPLGVVRRTRSKAPRLWPGVLILVGIFAPIVIRPLGEWLGHTSGGIGGRLVVLVGFAVVLLAVAGVVIGTGWISYTAGRLLHRFGRRPAALLAGRRLMADPWNGSRTMSALLAALVVGGGVVGYRIYMTTQFAANDAANRLNGTEDGYGDGTDFYLNSVDLIRIAVFAGIVVAAAGIMVAVAEGIVSRRRTNASLVAAGVPRKVLGESLAWQTFAPLVPAVLVALTVGISLLRGIATEASAGGGTTCDGTQAQCADPDSPLWKTYREVTLQVPVPFGDLAVLGGGALVLMLLAVGVGLIFLRRSTELDELRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ46</accession>
    <name>U5VQ46_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_03225</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03225"/>
    <dbReference type="EnsemblBacteria" id="AGZ38934">
        <property type="protein sequence ID" value="AGZ38934"/>
        <property type="gene ID" value="AFR_03225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.649"/>
    <dbReference type="eggNOG" id="COG1129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="257" mass="27463" checksum="551A42AEC046115D" modified="2014-01-22" version="1">MTTPVLEARGLTKRYGRVTAIESGDLELYPGEILAVIGDNGAGKSSLIKALSGAVVPDSGEIYLDGERVNFRNPMDARAAGIETVYQTLAVAPGLDIADNLFLGREERKPGVLGKVFRLLDKGHMRSEAKRHMSELGVATLQNINQAVESLSGGQRQAVAVARSAAFGSKVVILDEPTAALGVKEGNRVLQLIRDVRDKGLPVILISHNMPHVFEVADRIHIQRLGRRATVITPKSHSMSEAVAIMTGAAEPPPHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR86</accession>
    <name>U5VR86_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358895.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05375"/>
    <dbReference type="EnsemblBacteria" id="AGZ39364">
        <property type="protein sequence ID" value="AGZ39364"/>
        <property type="gene ID" value="AFR_05375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1088"/>
    <dbReference type="eggNOG" id="ENOG50339X8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363783_0_0_11"/>
    <dbReference type="OrthoDB" id="2011443at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="22679" checksum="4447471C304214C9" modified="2014-01-22" version="1">MSRDADGGLEGLFGRRADEVVVEEPVEIVEKRRSPIGWILRNVALIVVATVVTVAGLRSRGISVSVLLVVAAFVALRLLMFAVSEVAPPPPIRRQSRRGEEDGDYHWAGTDTLRAAVRRWEQRLDWSQNDAEKFSRNVLPVLAELTDERLRLKHGITRSSDPRRARELVGEPLWELLDDRDRRVPKARELSQYVDALEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRA4</accession>
    <name>U5VRA4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bulb-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05265"/>
    <dbReference type="EnsemblBacteria" id="AGZ39342">
        <property type="protein sequence ID" value="AGZ39342"/>
        <property type="gene ID" value="AFR_05265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1065"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3693">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_571904_0_0_11"/>
    <dbReference type="OrthoDB" id="654705at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="SSF51110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665238" description="Bulb-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="2">
        <location>
            <begin position="367"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="477" mass="51545" checksum="ED8A4CD17B192E00" modified="2014-01-22" version="1">MALALVAAAAVTAVVLPADAAETPVFTSGDVGFSEGGSLDGLAGRLDAMDAYVGDRKPMLRLDLNWSAVQPAATDEPRFTALDPVVDAAAARGIRVLLILNYGTTWTGGVHSFPSSDAAWRGIVDATVTHFGSKVQAYEVWNEPNYARFGDYGDGSAGVRRARYWQLVRIAHDRVHAGCAGCVVLAGASGVGDAVSETRNDNESAQWLDWAYANGYKNSFDAVAHHPYPAWNMGRSPSRPECTTRWWSMFGPPDAKCAELAAVRAVMVARGDSAKKIWGTEFGYPSTGTGITKLTPAAVRDNLEESVRMWRALDYTGPLFLYSFRDAAVCGPDPADAECHFGLVDTAGKPKQPAYDSLQLALTDTRLRTLAPGRSLHRASALRSTDGRLELWLRADGNLILYLGKTELWKKVGLQGVRLTNQTDGNLVLFDGAGKRVWSTGAEGRGVSDLDLQDDGNLVLYPRDKSKKLWSTNTYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRX9</accession>
    <name>U5VRX9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_06500</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06500"/>
    <dbReference type="EnsemblBacteria" id="AGZ39589">
        <property type="protein sequence ID" value="AGZ39589"/>
        <property type="gene ID" value="AFR_06500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1318"/>
    <dbReference type="eggNOG" id="COG1276">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023176_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032694">
        <property type="entry name" value="CopC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007348">
        <property type="entry name" value="CopC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008457">
        <property type="entry name" value="Cu-R_CopD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34820">
        <property type="entry name" value="PTHR34820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04234">
        <property type="entry name" value="CopC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05425">
        <property type="entry name" value="CopD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666007" evidence="9">
        <location>
            <begin position="50"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="CopC" evidence="10">
        <location>
            <begin position="48"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CopD" evidence="11">
        <location>
            <begin position="344"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ39589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="565" mass="58167" checksum="52E8FCB38D2AA949" modified="2014-01-22" version="1">MTVDLRGATDRSVRPARGRRARGPAAALGLLIGLFLVLLGPAAPASAHAALVGSDPASDTIVPDAPNKITLSFSESVQLISGKIQVLAPDGSRADQGEPQATGSTVVIPLRSGGGRGTYLVSYRVVSADSHPVAGSLTYSVGAASTPPTESAGAESVDPVVRALIPVGKYLGYAGLVLLVGPVLVLALLWPHRLSRTGPARLVWTGVGLVLGSTVLALWLQAPYTTGSGLFGVSGADLQDVLGSTFGAVMLVRLGVIIAAAFLLRPLLRGDGGESKADLALLGVLGVAALATWPLTGHPTASPVAGVSVVVDAIHLAAMAVWLGGLVMLVAFLLRQANERELGAILPIWSRWAATAVAALIIAGSVQALIEVASFKGLYASTYGRLILVKIGLAAVVIAMAAVSRNLVRSRTAENGPGRLRRIVMAELAVTAVVLGVTAALVQIPPPRTASAAESASTSTTISQTVADDSVSLQADIFPATVGNNSIHLYAYTLDNKPLPVVEWTATAALPAKNIEPIEVPLLRITDFHAVGDIALPMAGEWTFKFTVRTSDIDQSTLTMTAKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSC7</accession>
    <name>U5VSC7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glucan endo-1,3-beta-d-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01605"/>
    <dbReference type="EnsemblBacteria" id="AGZ38610">
        <property type="protein sequence ID" value="AGZ38610"/>
        <property type="gene ID" value="AFR_01605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.323"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032886_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037398">
        <property type="entry name" value="Glyco_hydro_64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032477">
        <property type="entry name" value="Glyco_hydro_64_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042517">
        <property type="entry name" value="Glyco_hydro_64_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38165">
        <property type="entry name" value="PTHR38165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16483">
        <property type="entry name" value="Glyco_hydro_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666019" evidence="1">
        <location>
            <begin position="17"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="403"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="520" mass="54072" checksum="AD85F5602B4F5120" modified="2014-01-22" version="1">MALPVAAVSVASPAQAIGPAILPVTVTNNTGRGEAVYVYVIGINLNTNRLGYVNQGGTFTPWPAGANPPSPAPDVAIGGPGNGGATTVNFPRGFSGRVYFSLGEKLKFFLTPDGLVQPAPWASGDANYNILFDWSEFTYNDSGLWLNSSQVDMFSVPHAVTVTGASGVTKKTGEPVNNGRNNVINAIKAAPGWANTVYTRSDGTVLRALAPGKAAGAGLLPTNYLDSYITSAWNAYVNKTLTVVPFGDQPNTKYFGRTTGNVMNFTNSAGARVASFNKPSSASVWGCDGDLPAPNDLIVGPISRTLCAALNRGTLGTIDTQPSTNAAQFYQNSPTNQYAKIIHQNMADGKAYAFAFDDVGAFESLVHDGDPRAAGLILTPFGAGGPTTPPPSGSGQSVISDWNGKCVDVPSGNFVDSAPLQTWTCNGSNAQKWTAVNGALQAQNNKCMDVAGGSTAAGSAIQLYTCNGTGAQQFVLSGAGDLVNPQSNKCVDIKDWNGADGARLQLWDCAGTANQKWHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSF0</accession>
    <name>U5VSF0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357494.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01310"/>
    <dbReference type="EnsemblBacteria" id="AGZ38551">
        <property type="protein sequence ID" value="AGZ38551"/>
        <property type="gene ID" value="AFR_01310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.262"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142293_0_0_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="14142" checksum="150B01BDC6AEE258" modified="2014-01-22" version="1">MSATFNHTIIASKNRHESAAFFHDLLEAEEAPAWGFFANLKLDGGVLLQFAEPPVDIQPQHYAFLLDDELFDRAYAKVRERGIEHWADPQMKRAGETNTEHGGRGVYFRDPAGHFVELITRPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSG3</accession>
    <name>U5VSG3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357593.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01805"/>
    <dbReference type="EnsemblBacteria" id="AGZ38650">
        <property type="protein sequence ID" value="AGZ38650"/>
        <property type="gene ID" value="AFR_01805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.365"/>
    <dbReference type="eggNOG" id="ENOG5030UNM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152365_0_0_11"/>
    <dbReference type="OrthoDB" id="2006504at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665270" evidence="1">
        <location>
            <begin position="24"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="112" mass="12084" checksum="9496A1C77796677C" modified="2014-01-22" version="1">MAKILLALLAAILLAGCSEPVQKVQQPVSTPPSTPTDQFQYDDLIVGTVNRGGSGPCYGLVTDDGRQFALYENQGRELIKGVRVQVRAEPSTLRIDCGPGELLQVNALDEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSY8</accession>
    <name>U5VSY8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">zf-RING_7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359599.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08895"/>
    <dbReference type="EnsemblBacteria" id="AGZ40068">
        <property type="protein sequence ID" value="AGZ40068"/>
        <property type="gene ID" value="AFR_08895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1811"/>
    <dbReference type="eggNOG" id="COG1579">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073076_0_0_11"/>
    <dbReference type="OrthoDB" id="833439at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003743">
        <property type="entry name" value="Zf-RING_7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02591">
        <property type="entry name" value="zf-RING_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-RING_7" evidence="2">
        <location>
            <begin position="202"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="37"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="27304" checksum="D1639C5BF04DF1D3" modified="2014-01-22" version="1">MKAAPQAQRRLLDLQAIDTALAQLAHRRKSLPELAEIATISRELSALEDQRVRAQVAVDDLDRDITRFEKDIEQVRTRKARDQARLDAGGALREIEGLQHEMATLNRRQGELEDAELELMEQRETAEQTLNDIRKKLTEASERRAAAEARRDEAFADITKEQEFKTQARAPLAGDLPGDLLGLYDKIRADSGLGAALFRAGRCGGCRIELYGADLNRVKSAPADEVVRCEECRRIMVRTAESGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTS9</accession>
    <name>U5VTS9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cation-transporting ATPase pacS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_04080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358050.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04080"/>
    <dbReference type="EnsemblBacteria" id="AGZ39105">
        <property type="protein sequence ID" value="AGZ39105"/>
        <property type="gene ID" value="AFR_04080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.824"/>
    <dbReference type="eggNOG" id="COG2608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134973_13_1_11"/>
    <dbReference type="OrthoDB" id="2061355at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000428">
        <property type="entry name" value="Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00944">
        <property type="entry name" value="CUEXPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="4"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="70" mass="7017" checksum="073EB3CB46847EF9" modified="2014-01-22" version="1">MPVTSTYTVSGMTCSHCVQAVTSEISGLPGVDAVQVDLATGAVTVTSEALLAEDAVRAAVDEAGYELADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUC0</accession>
    <name>U5VUC0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein glnR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360382.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10925"/>
    <dbReference type="EnsemblBacteria" id="AGZ40474">
        <property type="protein sequence ID" value="AGZ40474"/>
        <property type="gene ID" value="AFR_10925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2225"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_11"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="126"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="1">
        <location>
            <begin position="126"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="26969" checksum="AD9A18EBA7B00DFF" modified="2014-01-22" version="1">MEILLLATRPGAAAEDRALPALDLLRHSLRVGTYDVQALVSGADPDVVLVDARRDLLEARDLSRLLRTTGLRVPLLAVVEEAGLVAMAADWGLDDVLLTSAGPAEVEARLRLTTARVSGAAGGVSSGMVTAGELSIDPHTYSARLKGRTLDLTYKEFELLKFLAQHPGRVFSRDQLLREVWGFDYFGGHRTVDVHVRRLRAKLGSEYESMIGTVRQVGYKLVLPPQQSPRVLASEWDPIAPRVAVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUP7</accession>
    <name>U5VUP7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360541.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11350"/>
    <dbReference type="EnsemblBacteria" id="AGZ40559">
        <property type="protein sequence ID" value="AGZ40559"/>
        <property type="gene ID" value="AFR_11350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2313"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4978">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065103_2_2_11"/>
    <dbReference type="OrthoDB" id="1748122at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010499">
        <property type="entry name" value="AraC_E-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029442">
        <property type="entry name" value="GyrI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06445">
        <property type="entry name" value="GyrI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00871">
        <property type="entry name" value="AraC_E_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="28526" checksum="53E447CE1D83DA21" modified="2014-01-22" version="1">MLTIGEFSRLTHLSVRTLRRYHEGALLEPAVVDESSGYRYYTVEQIPTAQVIHRLRELDVPLGDVQRILRAPDPGVRATLVAGHLQRLEAELDRTRAAVTSLRRLLRPDPSPIEVELRAEPARTVAAVAAVVDEGEVDAWFAGAMAELEVAVGDAVTGPPGGSYDNSLFEQGRGHVLVYLPTAAPPRTGRVHAVTLPAAELAVATHIGEHDGVEVTYGELGAWVVGNAMSVAGPVREVYLVGPRDTGDPAAWRTEIGWPVFRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV02</accession>
    <name>U5VV02_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">D-malate dehydrogenase (decarboxylating)</fullName>
            <ecNumber evidence="5">1.1.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_12710</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-malate + NAD(+) = CO2 + NADH + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:18365"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.83"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.83" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360887.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12710"/>
    <dbReference type="EnsemblBacteria" id="AGZ40828">
        <property type="protein sequence ID" value="AGZ40828"/>
        <property type="gene ID" value="AFR_12710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2581"/>
    <dbReference type="eggNOG" id="COG0473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_11"/>
    <dbReference type="OrthoDB" id="1551125at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046553">
        <property type="term" value="F:D-malate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011829">
        <property type="entry name" value="TTC_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02089">
        <property type="entry name" value="TTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="7">
        <location>
            <begin position="5"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="360" mass="39034" checksum="CE5B932718C9F1DD" modified="2014-01-22" version="1">MTTHRIAVIPGDGIGKEVVPAGVSALQATADRFGLSLEFDEFDFASADYWQKHGTMLPSDWEQTLRQYDAIFFGAVGWPSVVPDHVSLWGSLLQFRRTFDQYVNLRPCRLMPGVTSPLAGREPGDIDFLVVRENTEGEYSSVGGRIFEGTPRETVLQETVMTRVGVDRVLRFAFEQAARRPKKHLTSATKSNGISISMPYWDERVAEMGSKFPDVRLDKFHIDALAAQFVLHPEWFDVVVASNLFGDILSDLGPACTGTLGIAPSANIDPSREAPSLFEPVHGSAPDIAGQGIANPIGQIWCGSMMLDHLGHPEAAAHLLGAIEAVLAHGPAGAPLTPDLHGTGTTAELGHAITECVRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVK0</accession>
    <name>U5VVK0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_07230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359266.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07230"/>
    <dbReference type="EnsemblBacteria" id="AGZ39735">
        <property type="protein sequence ID" value="AGZ39735"/>
        <property type="gene ID" value="AFR_07230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1476"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_11"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="29"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="298"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="392" mass="40006" checksum="0DB3FBAD0AEC5455" modified="2014-01-22" version="1">MALSPARLGLADLVRVSLSGLRARPLRAVLSALGIAIGIAAMTAVVGISSSSRADLDQQLAALGTNLLTVGPGNTMFGESATLPDESVAMISRIGPVSAVAATGVVTDAAVYRTDRIPPAQTGGVSVGAAQLDLLPTVGASMASGAWLNAATERYPAVVLGATAARRLGPVPSVYVSGRWFTVVGVLDAVLLAPELDSAVLMGWPAAEEYLDFDGHPTTVYTRSSDAQVTAVREVLAATANPSNPDEVEVSRPSDALQARQATDRTFTQLLLGLGAVALLVGGIGVANTMVISVLERRAEIGLRRSLGATRGQIRVQFVAESLLLSLVGGLGGVVIGSVVTAFYASTRSWPTVVPLWALLGGVVATVVIGAIAGLYPAVRAARLAPTEALTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVV1</accession>
    <name>U5VVV1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongation factor 4</fullName>
            <shortName evidence="5">EF-4</shortName>
            <ecNumber evidence="5">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ribosomal back-translocase LepA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">lepA</name>
        <name evidence="7" type="ORF">AFR_07805</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359381.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07805"/>
    <dbReference type="EnsemblBacteria" id="AGZ39850">
        <property type="protein sequence ID" value="AGZ39850"/>
        <property type="gene ID" value="AFR_07805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1594"/>
    <dbReference type="eggNOG" id="COG0481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009995_3_3_11"/>
    <dbReference type="OrthoDB" id="182107at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="PTHR43512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="18"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="30"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="151"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="624" mass="68917" checksum="A0A395A5A0968136" modified="2014-01-22" version="1">MPGPLDPGVNIIGATDPRRIRNFCIIAHIDHGKSTLADRMLQITGVVDPRQMRAQYLDRMDIERERGITIKSQAVRMPWVVREGGRQGDTAVLNMIDTPGHVDFTYEVSRSLAACEGAVLLVDAAQGIEAQTLANLYLAMENDLHIIPVLNKIDLPAAQPEKYAEELAKLIGCEPDDVLRVSGKTGEGVAHLLDEVVRQFQPPVGDATAPARAMIFDSVYDVYRGVVTYVRVIDGRIEARDKIKMMSNGTVHELLEIGVVSPEMEKAGALGVGEVGYLITGVKDVRQSRVGDTVTNNQRPAKEALGGYSDPRPMVYSGLYPIDGSDYPALRDALDKLQLNDASLTYEPETSAALGFGFRCGFLGLLHLEIIGERLEREFNLDLISTAPNVVYRLFTEDATEHVVTNPSEFPDGKIAEIHEPVVRATVLVPNEFVGAVMELCQSRRGQLLGMEYLSTERVELRYTLPLGEIIFDFFDQLKSKTKGYASLDYEPSGEQVADLVKVDILLHGEPVDAFSAIVHKDKAYNYGVTIASKLQKLIPRQQFEVPIQAAIGSRIIARETIRAIRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKMVGRVEVPQEAFIAALSTSDSGDNKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW01</accession>
    <name>U5VW01_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminopeptidase N</fullName>
            <ecNumber evidence="4">3.4.11.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alanine aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Lysyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AFR_07710</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.</text>
            <dbReference type="EC" id="3.4.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.2" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359362.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07710"/>
    <dbReference type="MEROPS" id="M01.012"/>
    <dbReference type="EnsemblBacteria" id="AGZ39831">
        <property type="protein sequence ID" value="AGZ39831"/>
        <property type="gene ID" value="AFR_07710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1573"/>
    <dbReference type="eggNOG" id="COG0308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007335_1_1_11"/>
    <dbReference type="OrthoDB" id="771837at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012778">
        <property type="entry name" value="Pept_M1_aminopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="SSF63737"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02412">
        <property type="entry name" value="pepN_strep_liv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M1_N" evidence="15">
        <location>
            <begin position="98"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M1" evidence="13">
        <location>
            <begin position="223"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1_C" evidence="14">
        <location>
            <begin position="523"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AGZ39831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="836" mass="91933" checksum="00A693E4A84CFA5A" modified="2014-01-22" version="1">MPSLTRAEAIARAATVAVTAYEIDLDLTAAGDTFRSRTVLRFDAKAGETTFVELEPAELISATLNGTPVDASALAEERLALTGLAQSNELVVEALMTYSNTGEGLHRFTDPADDRVYLYAHMFLDGARRIFPCFDQPDLKAPFTLHVTAPDGWLVAANGEPATEPAGGSWQFAPTKPLSTYFVSLIAGPYHARTDEHDGIPLALYCRQALAEHLDEQADEIFTITKQCLDRYHQIFDVRYPFGHYQQAFVPEFNFGAMENPGLVVFRDEFIHRSAVTDGEREQRAMVIAHEMAHQWFGDLVTMAWWDDLWLNESFAEYLGTRITAEATRFTETWTTFAMHRKAWGMRADQRQSTHPVAPSDVDDTALALLNFDGISYAKGAAVLRQLVAWVGDEAFLAGLNEHFAAHAYANATLADLLAAVSKASGRDLSAWADLWLRRAQVNTLRAEVQRDADDRYADVTIVQTAPEQYPTLRPHRIGVGLYDRGPDGVVVRRRRLDVDIDPAVDGGRTPLPELTGEPAADLLLINDGDLTYAKVRLDEASAAAVPMILPLLDDSLARALVWSATLDAVEDGERPVAELVTLVLAALPVEREVVLVEDVLRMTRVLVDRYPTPETRPAALELLAQASDRLVAASEPGSSRRLAGVRGLVGTSVDTARLRGWLDGEGVPEGVLVDDDLRWLILYRLVVLGAAGPEDIEATLAADRSAAGEQQAARCRAALPDPEAKARAWDLIINDTTSSNRILEATAHGFWQPEQLALVTPYVERYFAEMPGMLRRRTGMSGERIAAQAYPSLVVTPETRRHAAELLATPDLSSILRRVVTDGDDDVRRALRARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWB2</accession>
    <name>U5VWB2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH:quinone reductase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08680"/>
    <dbReference type="EnsemblBacteria" id="AGZ40025">
        <property type="protein sequence ID" value="AGZ40025"/>
        <property type="gene ID" value="AFR_08680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1766"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="3"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="27205" checksum="641CEBEBF4BB2C51" modified="2014-01-22" version="1">MIRTEDVPVPAPGPGEVLIRVVATSMNPTEAALRAGYLAALLPIELPLTLGWDVAGTVDGAPVLAMLDGGAAAEYAVAPAAALVPAPATVPLADAAVLPLAGMTAWQAVFEHARVAPGERVLINGAGGGIGGFAVQLARHAGAHVIATASPRSAAAIEADQLLDYTAGPLAVDEPVDVVLNCAAISAGAAAALPALARPGARIVTVATPVPGGRHFVVRNDPADLSALVKMVDEGALRLTITGRRTLNDLPELHRLAESGGLHGKVVVTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWN4</accession>
    <name>U5VWN4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Spermidine/putrescine ABC transporter ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_15680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361477.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15680"/>
    <dbReference type="EnsemblBacteria" id="AGZ41418">
        <property type="protein sequence ID" value="AGZ41418"/>
        <property type="gene ID" value="AFR_15680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3184"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_11"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="13"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="363" mass="39721" checksum="DC47FC5F5D1F8F4E" modified="2014-01-22" version="1">MNSPAIGATGARVELVDLCKRYGTAEPPAVDSIALDIQPGEFITLLGPSGSGKTTTLNMIVGFTEPTSGRIELNGKDISRTPAHKRNFGMVFQNYALFPHLTVAQNVAFPLRERKVPAAQSAKLVGDALELVDLGGMGNRRPHELSGGQQQRVALARAVVFSPSVLLLDEPLSALDRKLRQSLQREIKRLHDELGLTFVFVTHDQDEAMTLSDRIAIFDHGKIVAVGTPAELYHRPTTQFVARFLGESNVFAGRYRSADVYEWNDRKWTVRETGDGTENQALIVRPERLQVAFDEESVPSGSNVAPAAVTDVSFYGTYYRIELAFDDNSTGMAVLPVGAPAVVDPGTRVIAHWRPEDQVLVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWW2</accession>
    <name>U5VWW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359698.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09185"/>
    <dbReference type="EnsemblBacteria" id="AGZ40126">
        <property type="protein sequence ID" value="AGZ40126"/>
        <property type="gene ID" value="AFR_09185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1870"/>
    <dbReference type="eggNOG" id="ENOG50346NH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109391_0_0_11"/>
    <dbReference type="OrthoDB" id="1987433at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665430" evidence="1">
        <location>
            <begin position="33"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="149" mass="15235" checksum="ADB00905B6171670" modified="2014-01-22" version="1">MKLSTRFVRRAALASAAAAGAAVLLSPGAAFAATDDSFTVKTTNGCGSVEFVDYGPGAAGGGNNDDYLVIHDYCSDGHGVTAFSTVNNGEQKSEYNGNGLAGAAVIWDPFAPRNINAGDTIKLKVCLFDGPADETGQYCKTVSKTSVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX02</accession>
    <name>U5VX02_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uridine kinase</fullName>
            <ecNumber evidence="1">2.7.1.48</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.48" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09780"/>
    <dbReference type="EnsemblBacteria" id="AGZ40245">
        <property type="protein sequence ID" value="AGZ40245"/>
        <property type="gene ID" value="AFR_09780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1994"/>
    <dbReference type="eggNOG" id="COG0572">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090613_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="20580" checksum="B7FDCC80B7C40CE5" modified="2014-01-22" version="1">MAVDGVDGVGKSTFAAELAAVLEERGRPVVHVSADGFHHPRAVRHRRGARSPEGFWLDSYDYAALIANVLNAGELYRPAVHDVRTDEVLDLPWQVRPDGAVIVVDGLFLHRDELVAYWDFSVFLTAPFAVTVARMAARDGSEADPDHPSLARYVQGQRLYFAACEPWRRATIVVDNSDLDRPRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX26</accession>
    <name>U5VX26_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15710"/>
    <dbReference type="EnsemblBacteria" id="AGZ41424">
        <property type="protein sequence ID" value="AGZ41424"/>
        <property type="gene ID" value="AFR_15710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3190"/>
    <dbReference type="eggNOG" id="COG0599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070025_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="55"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="17474" checksum="5C02605F4D0FE31B" modified="2014-01-22" version="1">MIVVRWRQHTTFGQERLMSDDESRLERGNKVRREVLGDAHVDRSMLAATDFTRVLQEYVTASCWGDVWSRPGLDRRTRSLLNLAMLTALNRPHEFQVHVRGALRNGCTEADIQEVLLQTAPYCGAPAALEAFRLAERVMIEVREAAAEPVEAVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX31</accession>
    <name>U5VX31_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09585"/>
    <dbReference type="EnsemblBacteria" id="AGZ40206">
        <property type="protein sequence ID" value="AGZ40206"/>
        <property type="gene ID" value="AFR_09585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1954"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="2">
        <location>
            <begin position="35"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="27345" checksum="59EF7F07A88D9EE6" modified="2014-01-22" version="1">MFYWLLKYVVLGPVLKCVFRPDVQGLAHVPRSGPVILACNHLSFSDSIFTPLLVKRRVTFVAKAEYFTGKGIKGWLMRQFFVSTGTIPVDRSGGKAARAALDTQLRVLRSGNIAGIYPEGTRSPDGRLYRGKTGVARLALESGAPVVPVALLNTDEIQPTGKLIPKIKRVKIRFGEPLDFTRYAASAGDRFVERAVTDEIMYELMTMTGRPYVDVYAASLKTAIVAPTPTPEGRTQSRTAPVGVEGAEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX79</accession>
    <name>U5VX79_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polypeptide N-acetylgalactosaminyltransferase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361547.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16010"/>
    <dbReference type="EnsemblBacteria" id="AGZ41484">
        <property type="protein sequence ID" value="AGZ41484"/>
        <property type="gene ID" value="AFR_16010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3249"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023845_0_5_11"/>
    <dbReference type="OrthoDB" id="957193at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="158"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="310" mass="33118" checksum="E903F26E251B3D85" modified="2014-01-22" version="1">MVSTAETVAVVVVTYNSERLIPDLLASLGPGLEGIDWHLTVADNASADDSVGAVRRLAPDATVVEMGRNAGYAAGINAAVAKAGPFTAVLVLNPDVRLTPGCIGTLLGTLRTPGTGIAVPLLVDGDGKLIQTMRREPSILRTLGDAVLGAGRAGRYPALGEVVTEERAYDIAAITDWAEGSTVLFSAECWERCGPWDESFFLYSEETDFALRARDAGLVTRFTPAAQAIHLEGDSRVSPRLWMLLTLNKVRLYRRRHNPVAAAGFWAALVLREGSRAALGKTPSRTAVRALLSPSRLREQPGPRMVHPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXC2</accession>
    <name>U5VXC2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16210"/>
    <dbReference type="EnsemblBacteria" id="AGZ41524">
        <property type="protein sequence ID" value="AGZ41524"/>
        <property type="gene ID" value="AFR_16210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3289"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_713384_0_0_11"/>
    <dbReference type="OrthoDB" id="821953at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665819" evidence="2">
        <location>
            <begin position="22"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="426" mass="44045" checksum="27F5264F4B74248E" modified="2014-01-22" version="1">MIPALTVVVTAALTVPGPALAAAVQRTERVSVSSTGVAGDDWTIVGDLSANGRYVVFGTRAANLVAGDTNNADDVFLRDRRTGTTTRISLAADGGEADGDSSEPAISDDGRFVAFRSAATDLVPGDTNGVADIFLLDRRSGATTRISVTADGTQADGDSGGVSISSDGRSVAFETVASNLAPGAGTGNVLLHDRTTGGLSLVSVEDGGDGWASAGGSYWASISGNGRYVAFVSYEADMVPGDTNDSRDVFVRDLVAGTTTRASLTHDNQQAADFVGGPDVSDDGRYVTFWSEDPTLVEGDTNGSADVYLRDMQTRTTRAISSSPQGVIGDQPSADPRITPDGRYVTFSGGATNLVAGDTNNADDVFVTDLRAGSTRIASRASNGRQGNDHSFNGRSTPDGRTVVYGSFATNLVRGGNDQTHLYLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXH9</accession>
    <name>U5VXH9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360318.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10755"/>
    <dbReference type="EnsemblBacteria" id="AGZ40440">
        <property type="protein sequence ID" value="AGZ40440"/>
        <property type="gene ID" value="AFR_10755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2192"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055817_1_1_11"/>
    <dbReference type="OrthoDB" id="1693430at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043917">
        <property type="entry name" value="DUF5753"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19054">
        <property type="entry name" value="DUF5753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="31688" checksum="68D2DFEB85964CB0" modified="2014-01-22" version="1">MPEFIGPAIARDRVRTRLRELREARSLQVGEVERAVHWPASALVDVESGTRLIEPLELETVLSHYGCDDSETIELVRLVRIARTPLSQGRHQLTGEYLQFAGYESEASRITHYQPLIVPGLLQTTAYALAATATILGAGPTDSDVVAVVQVRRERQRVVESRLATDDLPAIVAIVDEVVLRRRIGGDDVMREQLEHLSTQAARAHVELIIMPTAVGGHVGLGGVFELLEFPDSRDDDLVFIESATGDTLSRDPEVTTRYRGKADRLRATGLSEDNARSLIQQVLDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXJ0</accession>
    <name>U5VXJ0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mak_N_cap domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360334.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10805"/>
    <dbReference type="EnsemblBacteria" id="AGZ40450">
        <property type="protein sequence ID" value="AGZ40450"/>
        <property type="gene ID" value="AFR_10805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2202"/>
    <dbReference type="eggNOG" id="COG3281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108515_0_0_11"/>
    <dbReference type="OrthoDB" id="1568624at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040999">
        <property type="entry name" value="Mak_N_cap"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18085">
        <property type="entry name" value="Mak_N_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mak_N_cap" evidence="1">
        <location>
            <begin position="20"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="19410" checksum="752CD3C53CCB108E" modified="2014-01-22" version="1">MALLYRTELTPSKLDLLAAWLPGQAWFDGAGADGMRRVAAYRIDDPAGEVGVETMLVRVGDGPIHQVPLTYRGAPLDGAEAWLIGTAEHGVLGRRWIYDGLGDPVFHAGLADAIAGAPQAEEYLEVDGRRELRPATMTITGSGVAPGDRPVTVVRQPGESTRASGATLTGVVAGLPAPLLLAYAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXJ9</accession>
    <name>U5VXJ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative HTH-type transcriptional regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10855"/>
    <dbReference type="EnsemblBacteria" id="AGZ40460">
        <property type="protein sequence ID" value="AGZ40460"/>
        <property type="gene ID" value="AFR_10855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2211"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083309_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="26797" checksum="A6F6161B73CF127E" modified="2014-01-22" version="1">MGVLLKRWRGDRRLSQLALAGRAGVSARHLSFVETGRSRPTRDMILRLAEELAVPLRSRNELLLAGGFAPAHPEGDLGGPGLAAVLASLRAVLEGHEPFPAVLLDRHWTMIDGNAAVPLLTAGAAPELLEPPVNVLRLSLHPDGMAPRILNLAEWRAHVLHRLDRQAQATGDPVLRELHDELLALPGGLAAAQPDGLVVPLRFAAGDRELSFFSVTSVLGTPLDVTLSELAIESFLPADPDTARFLRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXR6</accession>
    <name>U5VXR6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17500"/>
    <dbReference type="EnsemblBacteria" id="AGZ41778">
        <property type="protein sequence ID" value="AGZ41778"/>
        <property type="gene ID" value="AFR_17500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3547"/>
    <dbReference type="eggNOG" id="ENOG503355G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146479_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="149" mass="15311" checksum="0B4CBBB9770E5095" modified="2014-01-22" version="1">MITHEIGFTGEVSPVHKWRVLTLLLVSAGLSVAGCGAGGDRESAADAVAVRLLTAVGARDGVTACSALAPETASEVSRNAGKPCPEAVLEEDLPAPGQVTGTDVYGQWAQVRLSDDTVFLAVFPDGWKVVAAGCTSRGERPYLCAVQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXZ0</accession>
    <name>U5VXZ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16795"/>
    <dbReference type="EnsemblBacteria" id="AGZ41637">
        <property type="protein sequence ID" value="AGZ41637"/>
        <property type="gene ID" value="AFR_16795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3406"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_7_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13463">
        <property type="entry name" value="HTH_27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15446" checksum="419477C74B272AAB" modified="2014-01-22" version="1">MTTALELVRWIGWAQRKAGEDWIRARELSHEQAFVLGYLVRNPGAIQRDIAEVSRTSAASVSSLLQGLERRDLVERRTEPGNERSKRVHATAAGAELIAGFESAMAAADETILAPLDETERATLQTLLNKITAQLPQPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYG3</accession>
    <name>U5VYG3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cellulose synthesis regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18255"/>
    <dbReference type="EnsemblBacteria" id="AGZ41929">
        <property type="protein sequence ID" value="AGZ41929"/>
        <property type="gene ID" value="AFR_18255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3705"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_528576_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="387"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="515" mass="56615" checksum="5BF530E09ACC7049" modified="2014-01-22" version="1">MPDTRLPVPVEPYGVFVALAREVHDRTMNGHHDEALVLADRAEAIAAILGDERAQLMIRQGRMYALLALGRLSEALVIGQTLAEDRQLVGPRASQAKALADLAELLIKLGRIDEGLHWLARATTALDLAPHDSLRYFSAICSVGDAAQAAELYELADDCARLAADALAVDELFRAAGRLQHAEMLLEWALRLEQVGRATEARLRLDRSVALMREHLKAHPDAPLATAMLALGLARTGRPDDAIVLIRRMLLPLRAAGQTHEVRLLHLAYGSALRALGDRAGAHREFTAAQELAGQTGQRLIIGYELADLAGERAPSEATRTMKATLREQVGHLWRQRLDRRLMLQQARRRAELELDRERAEGVAASDALTGLGNRRTFDRRLGELHGGTSLILIDVDKFKGINDAYSHGVGDGVLRGVAEVLRAHCREGEVAVRFGGDEFALFLLADLPAARRIAERIRRVVVRRDWDELVPGLRVTLSMGLAAYSEGMTGSELFDRADTQLYAAKRQGRNRLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYQ6</accession>
    <name>U5VYQ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta fold family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360855.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12550"/>
    <dbReference type="EnsemblBacteria" id="AGZ40796">
        <property type="protein sequence ID" value="AGZ40796"/>
        <property type="gene ID" value="AFR_12550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2549"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_412597_0_0_11"/>
    <dbReference type="OrthoDB" id="665798at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="536"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="601"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="625"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="105"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="665" mass="69874" checksum="86D9DBA42D384EC1" modified="2014-01-22" version="1">MEDIGPRRRALLRTAALVATAGIIIALAFLTVRPGSTLAVPDGARAGELSMSPCDYRTEAGTRAAECGTLVVPENRNDPAADLIALPVIRIPAEVEQPREPVFRLGGGPGSTNMKFPEASRLTADRDVVLVGYRGVDGSRRLDCPEVAGLRHAEGDLTGAGSQQAARLGFTACARRLTGDGVDLRGYSVAQRVDDLEAARTALGYQKINLISSSAGTRTAMVYSWRHPTALLRSAMVGVNPPGHMVWDLAITDKQISRYARLCAADTRCSTRTDDLAASMREAATDMPRRWGPFAIHAGSVRAASMFGMHHNGPGSAPLNAPTVIDAYLNGPGALWAMSVLGEVQLPDSIVWGEFASFAMIDAPAAQSFYQAGGDPGSVLRSDGTNFLWGGPSGFSTVWPDSPDNAPYRAPQLSTVETLLVSGTLDFSTPAETATDDLLPLLPKGHQVILPELGHSADFWDNADASRHLLNTFYDSGRIDDSKFGTRPVAFDPGPLTMSTIAAILLGVAVIAAVLGLGLLTVLVRRARRGGFSHRAGMWLRILTPLPLGLGGWFLAVLLIWSLDLNTFIAGTSAVIPAVSVAVGLGTWAAWVRHNRPRRTALAVALGAAVAGALLGYSAGATLTAPLTAIAGAAAAANLGLLILDHRLHRPRAAVASESPVPASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZN2</accession>
    <name>U5VZN2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361160.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14075"/>
    <dbReference type="EnsemblBacteria" id="AGZ41101">
        <property type="protein sequence ID" value="AGZ41101"/>
        <property type="gene ID" value="AFR_14075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2855"/>
    <dbReference type="eggNOG" id="ENOG502ZKTM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047832_0_0_11"/>
    <dbReference type="OrthoDB" id="1661570at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="437" mass="46100" checksum="E571F24E9A5CCAC6" modified="2014-01-22" version="1">MTRILRIELRRSGVAWMGVLLLVAGTWLLYAAPYRWTSGYMILAMDQRWYLSFLTGLAVAAGAWQGRREHRSGVLELFAGVPRPRAQQVAPILTVYGITAFVTYAGAWGLAAVSIVDTAEYLRAGAVAGVLVAGALAMVTAAWFGLAAGRLLPYLATAPLLAVATFATPLVAKGITGNREWLSALLFPSSTLGGPDDFATVPGRFSVAQVLYLTGLAASVALLFAVARKRALLPALLGIIAAVLVLQGGSAFVKEPIDAAARELVCTADTPKVCVARVHSGVLDEVTPPARAALAKLARLPDAPTSAEENIIPQLDIANYPDSMPIPMSIGDDGHVYEAGNLEHIMVSNVGVLPFVCPDESPGTDPVVVNAATAWLLGVDPAQLDLATEDVAPARKVWLQLRKLDEREAAARVSAVRRAVLSCSSGDDLLARPAALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZN5</accession>
    <name>U5VZN5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ASCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362604.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42232">
        <property type="protein sequence ID" value="AGZ42232"/>
        <property type="gene ID" value="AFR_19800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4018"/>
    <dbReference type="eggNOG" id="COG4405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102450_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009326">
        <property type="entry name" value="DUF984"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39203">
        <property type="entry name" value="PTHR39203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021320">
        <property type="entry name" value="DUF984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="1">
        <location>
            <begin position="11"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="15437" checksum="53558C24058BB0C7" modified="2014-01-22" version="1">MDIDAMPRAYFAFPGPLRDQLVGAILSGAKTSTSSLLADYEHEGEALPVVGEVSVVVDSDDQPVAVIETTGVHTVRLGDVDLQHAIDEGEGYTTVAEWRTGHENFWHSPEARASLGDPAFTVDDDTLMVAERFRVVTTLRPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZV8</accession>
    <name>U5VZV8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclophil_like2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361245.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14520"/>
    <dbReference type="EnsemblBacteria" id="AGZ41186">
        <property type="protein sequence ID" value="AGZ41186"/>
        <property type="gene ID" value="AFR_14520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2949"/>
    <dbReference type="eggNOG" id="COG4925">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099043_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041183">
        <property type="entry name" value="Cyclophilin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18050">
        <property type="entry name" value="Cyclophil_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclophil_like2" evidence="2">
        <location>
            <begin position="5"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="119" mass="12258" checksum="C616A7EE42B79E76" modified="2014-01-22" version="1">MKISITIGGERFSATIQDSAAGRDLVAQLPLTIDMTDHGSVEKTGPLPSPLSLAGQPDGADPEVGDVGYYAPGNDFVLYYGDQSYFPGIVIIGRLDGDAAGRIAGLEGAVTATVDVYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W023</accession>
    <name>U5W023_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_14920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361325.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41266">
        <property type="protein sequence ID" value="AGZ41266"/>
        <property type="gene ID" value="AFR_14920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3030"/>
    <dbReference type="HOGENOM" id="CLU_2630116_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="77" mass="8407" checksum="B7184E3E5ED3C55E" modified="2014-01-22" version="1">MIRDRHHAATIGARRRPNVIDVGQIQPVIRDCTNAATSSGARRRPNFTDVGQIQPVIRDWTNAATSSGTCRKPDLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W029</accession>
    <name>U5W029_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fur family ferric uptake regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361394.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15265"/>
    <dbReference type="EnsemblBacteria" id="AGZ41335">
        <property type="protein sequence ID" value="AGZ41335"/>
        <property type="gene ID" value="AFR_15265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3100"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_4_0_11"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16434" checksum="C254EBAD2D0D7618" modified="2014-01-22" version="1">MTSDFEAQLRAVSLRVTRPRLAVLAALRDHPHVDTDRVIELVRAGQPTVSHQTVYDVLRALTDSGLVRRIQPAGATARYESRVEDNHHHVVCRSCGAIADVDCTVGHAPCLTASDDHGFVVDEAEVVYWGTCPGCATTRMTSWPASSEGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0H9</accession>
    <name>U5W0H9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21645</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362971.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21645"/>
    <dbReference type="EnsemblBacteria" id="AGZ42599">
        <property type="protein sequence ID" value="AGZ42599"/>
        <property type="gene ID" value="AFR_21645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4387"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_50_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="336"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="1">
        <location>
            <begin position="478"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="728" mass="77869" checksum="614043721DB7D6C9" modified="2014-01-22" version="1">MPTTWSTLQLTEFFSAITRAGDVAGAATLAVERAAEATEAELAAVVRGGELIACLGLGRQPSGDLLAGVTSGAETFTVPGFGTFHSTLHPLGRDTEGFLFLGRLDDEFEAEERQMLQGMAKVLGLALRGLETLAAERDLRREREREAEARLVLVNALEKREQLLGTLLQIQRAVSQRAPLQEVLDSVTRGASGVLEGALAALVLRDADAADETMVASLTPGHEDSRHDPQVLAVAVEAMQRDHPVDHDVVVAAPVHVGGDVAGSLVLGRCGDTIRSQERRDVLAAFAEQASLALTGAHSVAAVRQAYHDGLTGLPNRTLFLERLDRALEVAHRTRTAIAVLFLDLDLFKQVNDTLGHAVGDELLRGVASRLSTAVRGGDMAARLGGDEFAILLEPVDGGDQAREVADRIIASIARPFEIGGKEVLTRASIGIALGDAACTTAGDLVQDADVAMYRAKKNRPGTSAMFEPGMRTELLRHVELGTDARRAFESGEFRLAYQPIVALPGGRITAVEALLRWTHPRLGPVAPDTFIPLAEQSGLIIDLGRWVLREACRDLAGWRAAGLPDLTVSVNLSSRQLQDEQLVADVTAALAETGVPASALTLEVTETALMRDPREVQRRLRALKSIGVRLAVDDFGNGYSSMAQLSRFPIDQLKVDKSLIDGIGTDRRQRAIARSVLELGAILELETVAEGVEEPAQLAALTALPCTLAQGFLFAEPVRPEQVGDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0N5</accession>
    <name>U5W0N5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UbiA prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_22665</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363266.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22665"/>
    <dbReference type="EnsemblBacteria" id="AGZ42803">
        <property type="protein sequence ID" value="AGZ42803"/>
        <property type="gene ID" value="AFR_22665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4594"/>
    <dbReference type="eggNOG" id="COG0382">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060108_1_0_11"/>
    <dbReference type="OrthoDB" id="1472516at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="27390" checksum="10B2CA59562B3F03" modified="2014-01-22" version="1">MKSPLRTLAELVRAPAALSVPGDVIAGAAAAGSLAPATAGLAGASVCLYWAGMAANDWADRDLDATERPERPIPSGRISAPAALGIAAGLTAAGLALAARSGGARALAVAGPLAAAVWAYDLRLKNTAAGPAGMAVCRALDVLLGASTGNPVRALPAALVVAAHTYTVTELSRREVAGSTTAALPAATLAATAGLAGAVAARSGTVTAVLAGEYATSYGRAQAKLFGDPSAPRVRAAVGAGITALPALQGALTARAGRTWAGVAVAAAAPLGRLLARKVSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Q3</accession>
    <name>U5W0Q3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OsmC/Ohr family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362939.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21485"/>
    <dbReference type="EnsemblBacteria" id="AGZ42567">
        <property type="protein sequence ID" value="AGZ42567"/>
        <property type="gene ID" value="AFR_21485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4358"/>
    <dbReference type="eggNOG" id="COG1764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106355_2_1_11"/>
    <dbReference type="OrthoDB" id="1984062at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019953">
        <property type="entry name" value="OHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33797">
        <property type="entry name" value="PTHR33797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03561">
        <property type="entry name" value="organ_hyd_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="13936" checksum="5C009E53D736EB73" modified="2014-01-22" version="1">MQAVYTASATATGDGRGGHTRSSDGVLDLDLAVPKEMGGPGGAHSNPEQLFAAGYAACFHSALKLVAGKQKITLTDTAITVDVGIGPNGQGGFGLTVAIEAELPGLDEATAQSLLEAAHQVCPYSNATRGNVDVALTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0U4</accession>
    <name>U5W0U4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dolichol-phosphate mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362998.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21785"/>
    <dbReference type="EnsemblBacteria" id="AGZ42627">
        <property type="protein sequence ID" value="AGZ42627"/>
        <property type="gene ID" value="AFR_21785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4414"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_13_0_11"/>
    <dbReference type="OrthoDB" id="1064289at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="PTHR43398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="22"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="29329" checksum="37DC0261D6E7676C" modified="2014-01-22" version="1">MSDANPTVSGSEGYPGIGRVLVVIPTYNEADNIRLITDRVRRAVPSVDILVADDNSPDGTGDIADEISAGDDHVFVLHRAGKEGLGAAYVAGFAWAKNKGYDAVVEMDADGSHAPEELHKLLDALRDADAVLGTRYIPGGSVHNWPIHRLLLSRGGNIYIRMALGMPFKDATGGYRAYRMPVLDAIDVATVASTGYSFQVELAWRTYKHGFRIAEVPITFTEREHGVSKMSGNIFKEQLLRVTVWGATARRDALLARLGRKPRQGSTWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0V5</accession>
    <name>U5W0V5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Luciferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23115"/>
    <dbReference type="EnsemblBacteria" id="AGZ42893">
        <property type="protein sequence ID" value="AGZ42893"/>
        <property type="gene ID" value="AFR_23115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4684"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_6_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="30549" checksum="3B6E531AA04D062A" modified="2014-01-22" version="1">MTAPMQVAYEDILRVWREADTIPEIEHAWLFDHLMPIGGDPAGPALEGWTLLPALAAQTSRIRLGVMVTSNRFRPPALLAKIATTVDIVSGGRLDFGIGAGSRPGHPLARREYAAHGLPFHDADHAVGSLAEACTVIRRLWTEAEPFDFPGTYVQLTGAFGNPKPVQRPHPPILIGGRSSKVLRIVAEHADLWNIPGGDLTDAASRSALLDRYCAEIGRDPATITRSIHLQVSYDEPGSTRTEIGLALDAGFTHVVLGLPSPYPASVARWVTDELITPSLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W131</accession>
    <name>U5W131_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Trifolitoxin immunity protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22920"/>
    <dbReference type="EnsemblBacteria" id="AGZ42854">
        <property type="protein sequence ID" value="AGZ42854"/>
        <property type="gene ID" value="AFR_22920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4645"/>
    <dbReference type="eggNOG" id="COG0510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066396_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="21289" checksum="50980500520157E7" modified="2014-01-22" version="1">MREETLPGETIGAGESAPWASADPMLEQVGRWLRRVHDLTADFPAPADEHWFIGGTVQPGLIVGHQDAAPYNAVVDGDRLVGFCDWDIAGPSTREWDLAFSALSWVPLASPWDGTRSDHGEQSRRLQLLLDAYGYDGDRHAFSTVVPQRARRQAGVIRSMAEAGDPASIGLLPVAEMLERSATDLEALPATFWNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W168</accession>
    <name>U5W168_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Drug exporter of the RND superfamily-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_23095</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23095"/>
    <dbReference type="EnsemblBacteria" id="AGZ42889">
        <property type="protein sequence ID" value="AGZ42889"/>
        <property type="gene ID" value="AFR_23095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4680"/>
    <dbReference type="eggNOG" id="COG2409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005108_5_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="519"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="558"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="632"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="182"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="679"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="701" mass="71584" checksum="A37388BB658F8872" modified="2014-01-22" version="1">MVAAWVLVATAGGVLAPRTVEALRYEFALPGQPAYEANQRITTATGGGGAVDPLVLVARPRQAGPPARAAFETQVARVTAAAPGSRVVTPAAAGELASPDGSVLVALVYPRVLPGPEPYAAALPALEAAAARTDVGGRPLTVTGTTVLEETSSGGRRGVLVEVVLGGLGALIVLAAVFGSLLAAVPLLVAVVSILATFLCLLGLTALTDVSFVVQYLVALIGLGVAIDYSLLIVMRWREERAGGLDGTAAVVAAMATAGRSVVFSGVTVAVSLAALVLVPLPFLRSIGLGGLLIPLLSVATATTLVPVVLATAGERLQWPRRRITDPSSRAWRRIATVAVRRPALVALASTLLLLGLAAPLLGLRLGSPRLDAYDASTPAGVATAQLTASGISPGVLRPTEVLLPPAEAPGAVARFAALPGVAAAVAPASAGWTTPAGQLIQVWTSEDPATATSRTAVRAVRDAAATIPGAQVGGHPAEDADFVTAVYSDAPFVVLGIVLVTFLLLARALRSLWLPIKALLLNVVSIAAAYGITVLIWQDGAGSDLIFGTPASGAITIWVPIAVFAFLFGLSMDYEVFILSRMREAYDEHGSTPRAVIDGIARTGRLVTSAALILFLAFVALSTVPAVEVKILATALALGIAIDALIVRALLAPALVVLLGRLNWSLPRPLAVLLRLPPEPSPRSRSTAAPKAPNGVSPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1W2</accession>
    <name>U5W1W2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24105"/>
    <dbReference type="EnsemblBacteria" id="AGZ43089">
        <property type="protein sequence ID" value="AGZ43089"/>
        <property type="gene ID" value="AFR_24105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4883"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2046009_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="119" mass="12714" checksum="CF128D895DA1D2AA" modified="2014-01-22" version="1">MMILIAGPYRSGTGGDPDLIAANLARLEEAAWPIFQAGHVPMIGEWVALPVLHRSAGAATAEDVLYPTAQRLLQHCDAVLRLPGKSTGADQDVAIARERGLPVYHHVEEIPGYAPQPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2B3</accession>
    <name>U5W2B3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25040"/>
    <dbReference type="EnsemblBacteria" id="AGZ43274">
        <property type="protein sequence ID" value="AGZ43274"/>
        <property type="gene ID" value="AFR_25040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5073"/>
    <dbReference type="HOGENOM" id="CLU_2550676_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="82" mass="8931" checksum="5CF75D26328495D2" modified="2014-01-22" version="1">MSMKTDRARRLAEAACEAGLVLGLVALPTAWRALDTSLMAVLAGMLLALTAATYALHAVTFDCFLLTGQRSRRNLSYTQRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2J2</accession>
    <name>U5W2J2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25945"/>
    <dbReference type="EnsemblBacteria" id="AGZ43453">
        <property type="protein sequence ID" value="AGZ43453"/>
        <property type="gene ID" value="AFR_25945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5260"/>
    <dbReference type="eggNOG" id="ENOG502ZBYF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069617_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="239" mass="25213" checksum="8BCA272B66BC2495" modified="2014-01-22" version="1">MSVERICLLVGVALIGSGLIHLAILIISGGSWEGPVSWRKPATFGVSFGLTLITVVWVSAYLPLRDRMRRILLAVFALDCCVEVGGITLQAWRHVPSHVNRETAFDSGVSTVLAAGGGILVVVLGLLAVAAFRARAAPSMRLAIRAGFVTLMIGLASGAAMIARGVVAVNAGDQQRAYQVLGFLKPVHGVSLHGILVLPAIAWLLTFTSWDEARRTRLVWLAVIAYGVAVAGALIVSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W343</accession>
    <name>U5W343_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dynamin_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25865"/>
    <dbReference type="EnsemblBacteria" id="AGZ43437">
        <property type="protein sequence ID" value="AGZ43437"/>
        <property type="gene ID" value="AFR_25865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5243"/>
    <dbReference type="eggNOG" id="COG0699">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019977_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="455"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Dynamin_N" evidence="2">
        <location>
            <begin position="42"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="582" mass="63852" checksum="442024C93030C80C" modified="2014-01-22" version="1">MATDLLAELDGARRLAITDDRPDLAERLERAAERLRVTDVTVAIVGEFKQGKSTLVNALLRTDICPVDADIVTAVPTVLRFGTPPSVTLQIPAEDGTLNPVGVPFDQLRRYVTEESSAGDATPRSVEVRLDRRLLGAGLSFVDTPGVGGLDSAHGNLTLGALTTADAALFVTDASQELTAPEVDFLRRILDRCPHVYCVVTKTDLHGEWRRIVELNAGHLAKQGLDVPIVAVSSFLRLRAQARDSTELNTESGFPRLVELLRRDVLGAARSGLQAAARAELAFVVAQLRERVEAEQAAAASPAAAEAITHRYAEKQRRTRALGSWQTVLGDGIQDLTADVDHDLRERLRGLLRGGEELLDESDPKETWRDFQAWASREATAAAVDNLMLLVTRTEQLARDVAERFDLEYDSLDVDLPSVEQGLGKVGDLDVNFHKSGMQQFLGAFTAARVTYGGFYMLGALGAIFNVAVAAPIGLIAGLTLGRRLIKQERERQIQQRRQQAKVELRRYVDDVSFHVGRDSREAVRRTQRFLRDEFAARAAAVDRSTAATAAAVRETAVLPDAERQARARHLAERRTELDRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3F0</accession>
    <name>U5W3F0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative sugar ABC transporter substrate binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560071.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27360"/>
    <dbReference type="EnsemblBacteria" id="AGZ43733">
        <property type="protein sequence ID" value="AGZ43733"/>
        <property type="gene ID" value="AFR_27360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5546"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_10_1_11"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665657" evidence="1">
        <location>
            <begin position="20"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="403" mass="42593" checksum="FD40B8C51FD86A6A" modified="2014-01-22" version="1">MHRPVLATLMIVPLVLAAAACGGGGSDDSGDSGTLRVLDYYNNEPDKTVYQQALEKCGQQNGVTIERETVPGANLIAKVLQQSSSRTLPDVLMIDNPDLQQIAATGALAPVTDFGLSADGYAKGVVDASTYQGKLFGLQPVTNTIGLFYNKDLLAEAGVQPPTTWAELRTAAKTLTKGDRYGVAFSAPANYEGTWQFLPFMWSNGGDEKNIATPQVAQALQLWVDLLGDKSASRSVLNWTQADVNDQFKAGKAAMMVNGPWQFPVLNEDTKLHYDVVPIPAPQAGGTVVAPLGGETWTIPQTGSKDRQAKAAKVVGCLNADDSQLSLGKLRQTVPTKTALGEQFVAANPSMKAFTTAVQTARARTGELGADWPKAATKIYTGVQTALTGGAAPQQALQQAQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Q9</accession>
    <name>U5W3Q9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ANTAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560195.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27985"/>
    <dbReference type="EnsemblBacteria" id="AGZ43858">
        <property type="protein sequence ID" value="AGZ43858"/>
        <property type="gene ID" value="AFR_27985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5673"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074354_4_0_11"/>
    <dbReference type="OrthoDB" id="1958442at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ANTAR" evidence="1">
        <location>
            <begin position="121"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="19910" checksum="61841C385A6BA8EC" modified="2014-01-22" version="1">MSRHPLPPSARPSERSLKECLITLSGTPDEDPATERCLIEIAELTAYLIDAVTCASIDGCPEDNCTASLSVPLFAGRGRPLAALDLRSQDAGGLAPLTASVLAAYEDDLPDTAAMIHDGLCPGAVELVEGLAGAFDVRAIIQQAIGMLIADEGTTADRAYALLRSRVTKPGSSLTSVAAGIVHPGRNLAECD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3R0</accession>
    <name>U5W3R0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative magnesium/manganese-dependent protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364576.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26980"/>
    <dbReference type="EnsemblBacteria" id="AGZ43657">
        <property type="protein sequence ID" value="AGZ43657"/>
        <property type="gene ID" value="AFR_26980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5468"/>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014270_2_0_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="300"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="553" mass="58466" checksum="AE925AEE0ECFEEB2" modified="2014-01-22" version="1">MDLRTPVTPLLAPAPALLRAALDASAEAVLLCRASDDTILLANAAATELLPGLVATGGALARATAEQADSFTDEVAERHLHGRRQALDADHYAWYLRDITEEIRRAEAHEAEQARTAFLAEAGRRLSSSLHQRRCVRTTVELAVAHLADAAVVVLPTRHRQSEWVRLVQGEAAEEGTLRDKVLGEVPGLEEALGGFPPIPSRWLDASQVPAWLLPEGFGPVGALLVTPLPGNAEPAGALVLARRGPDAVFSAEDEMLARIFAARAGAAVSAAALYREQVDTTAILQADLLPPELPQPEGVELVGSYQAAKDALRIGGDFYDVFGPTEESPETVIALGDVCGKGPEAAVLTGKVRQTLRALRLVGSDPAAMLTVLNRALLGSSRQHRFVTLVVGAISRSEHGRVRLELATGGHPAPLVLRADGTVEAVPVTGTLIGVVPQILVRPATIELAPGELCLMYSDGLTEARGGATGNEQYGETRLRDALASCQGMPGAAAVERVRQLVSDWVHGGVRDDIAMLAVRAPARTKLSLMETGGTSASPFAIDATRRGRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3S2</accession>
    <name>U5W3S2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative PAS-sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_27030</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27030"/>
    <dbReference type="EnsemblBacteria" id="AGZ43667">
        <property type="protein sequence ID" value="AGZ43667"/>
        <property type="gene ID" value="AFR_27030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5478"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029843_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006189">
        <property type="entry name" value="CHASE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042240">
        <property type="entry name" value="CHASE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03924">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01079">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50839">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="CHASE" evidence="9">
        <location>
            <begin position="125"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="473"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="716"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ43667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="746" mass="78257" checksum="F012F5E091AFDF42" modified="2014-01-22" version="1">MIGLTATLLTVGALRTGQRESAARVMDQRTSVARVAVAGEAGRYRDLLQAVAAGLGTNARLTAADFAAATAPLEGAGLVGATSVAYVVATPPARIAATQTFWRGRGADALTLAPRGQHAEHYFSIFQRSLNNGGPAMTGLDVAASREASSALTEARRTGRPTISDTYVLLRDRNIPVAQQQLSFLFAAPVYSPAEDAGGLPEFRGWVALGLHGQDFLGGVLGVASQGLLDGELFATNGDGRRVLVAGYDARGRRDLTRQATFPVADRQWTLLTGADSQNLPGARSSMPAIVLIGGILLSAMLACLVWILATGRSRAREQVLVATAELRTAEAESRRQAGLLGAIMSSLGDGVGVVDENGAFLLHNPAAQALLGVSDDVDGPDSWQQHFGLFRADGRTPFPVEEMPLVRALQGEASDGVEMLIRNDQRPDGILVSVDGRPLDAGAAGRHGAVAVFHDITELRRYETDLAVFAGVVAHDLKAPLAVIRGHCETAADELAEAPEGPEVFEARAALDRIANAVDRMAALIDTLLAYTTSRDAPLKLTAVQLGPLVADVVEHRTEHPRPGDSPPPDFYLGPLPEVQADPAMLRHVVDNLIGNALKYVQVGKSARIDVTAAGGPPGWTRIEIADRGIGIPDDDKPNIFESFHRARTAAGYAGTGLGLAICRRIVERHGGTIGVADNPGGGTRFTFTLPLAARTRNALDRALAERAAAAGSVPPEIAGVGPIRPAEAEAAPPRTPEAGRAEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W434</accession>
    <name>U5W434_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_22005</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363042.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22005"/>
    <dbReference type="EnsemblBacteria" id="AGZ42671">
        <property type="protein sequence ID" value="AGZ42671"/>
        <property type="gene ID" value="AFR_22005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4461"/>
    <dbReference type="eggNOG" id="COG3336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054944_2_0_11"/>
    <dbReference type="OrthoDB" id="1553593at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019108">
        <property type="entry name" value="Caa3_assmbl_CtaG-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09678">
        <property type="entry name" value="Caa3_CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="26807" checksum="6F0EE9875F7E3754" modified="2014-01-22" version="1">MTGHAAGSGAALLPVVAAVLYEWSSLRVPRWPPWRAAAFMLGCATLVLGLALPVHDFTGHAAQHLLVAMVAPIGLVLGAPVTLLLNALPLPAARRLSGLLRSRPVRLVSHPLCALTLTAGGLALLYLTPLYAHSLADPALHDATTVHFVLSGFLFAWVVAGSDPAPHRPSVPARLILLGVAIAVHAGLSQLLYAGALVHVPATEADRQAGATLMYYGGDLAELALALLVVQSRATTRKVSSFGSAASGRIIPAASPARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4A8</accession>
    <name>U5W4A8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type 11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363310.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22750"/>
    <dbReference type="EnsemblBacteria" id="AGZ42820">
        <property type="protein sequence ID" value="AGZ42820"/>
        <property type="gene ID" value="AFR_22750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4611"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_10_2_11"/>
    <dbReference type="OrthoDB" id="1057444at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="49"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="20813" checksum="87A03A594CD7115C" modified="2014-01-22" version="1">MSRQRSRSESQEFFAARAATWDTKFGSDLPAYAAAVAEAGLRAGAVVADVGCGTGRALPALRAAAGPDGVVLGLDLTPQMLAVAAPRAREHGAHLVEADALSLPIAGGRLDAVFAAGLLTHLPDTDGGLRELARVTRTGGTLVLFHPIGRAALAARHGHTLRPDEPLAEQPLREAAGRTGWALTRYADADDRFLAIAARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4C2</accession>
    <name>U5W4C2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363335.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22825"/>
    <dbReference type="EnsemblBacteria" id="AGZ42835">
        <property type="protein sequence ID" value="AGZ42835"/>
        <property type="gene ID" value="AFR_22825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4626"/>
    <dbReference type="eggNOG" id="ENOG5033CN8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2902405_0_0_11"/>
    <dbReference type="OrthoDB" id="1996797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="63" mass="7418" checksum="605270A01FD156F2" modified="2014-01-22" version="1">MAARRRYVARGVPGGYQIWDNRARRWWGELYEVCPDDLLGELNGAAEPARITELLRRYRGLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4U3</accession>
    <name>U5W4U3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_23430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363535.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23430"/>
    <dbReference type="EnsemblBacteria" id="AGZ42956">
        <property type="protein sequence ID" value="AGZ42956"/>
        <property type="gene ID" value="AFR_23430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4748"/>
    <dbReference type="eggNOG" id="COG3973">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010312_3_1_11"/>
    <dbReference type="OrthoDB" id="114076at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="6">
        <location>
            <begin position="191"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="212"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="721" mass="79381" checksum="8142212D6867E39D" modified="2014-01-22" version="1">MRQVFALPERLAAKADPGQIAGDEQHFAAMTESLEQLYADLSDRLDAERKAPGGRGQQALTRDQEIHRLTARLRTLRRFSLDLCLGRMDNADGAEPVYIGRLGLTDDAGRRLLVDWRSPAAEPFFGATHANPMGLTSRRRYRWTRGRITDYWDEVFTPDGLASHAALDDQSAFIASLGTNRSARMRDVLGTIQADQDAIIRAGSRGALVVDGGPGTGKTVVALHRTAYLLYSDPRLGHRRGGVLFIGPHEPYLAYVADVLPSLGEDDVQTCTLRDLVPQGATAVAETDPEVARLKSSADLVAAIEAAVRFYEEPPTTPLTVTVDWSEIRLGAEDWAAAFETPTPGTPHNEARDEIWEELLTILVDKHDGDEPEDLLRATLAQDKELRTTFNRAWPLLDAADMVGDLWSVPAYLRKCAPWLSRDEIRTLQRADAQAWTVSDLPLLAAARLRVGDPETSRKQHQHKATVAAERAEMSRVVDDMLAAEHDREGAMTMLMGKRIDQDTGKIVDIRDDLIDDSPPPTTDPDGYDGPYAHIVVDEAQELTDAEWQMLLLRCPSRSFTIVGDRAQARHGFTESWEERLSRVGLDRISLASLSINYRTPSEIMAEAEPVIRAVLPDANVPTSIRTSGVPVVHGGLPELGPILDTWLTEHAEGVACVIGDPTVRETPRVRSLTPELAKGLEFDLVILVDPHSFGTGTEGAVDRYVAMTRATRQLVILTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4W5</accession>
    <name>U5W4W5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560590.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29960"/>
    <dbReference type="EnsemblBacteria" id="AGZ44253">
        <property type="protein sequence ID" value="AGZ44253"/>
        <property type="gene ID" value="AFR_29960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6068"/>
    <dbReference type="eggNOG" id="COG3154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169722_0_0_11"/>
    <dbReference type="OrthoDB" id="1792009at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SSF55718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="26"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="111" mass="11896" checksum="7C53381D98F903D2" modified="2014-01-22" version="1">MASVDECRQALHDLAARLDANAETRGKLDLDRTLACRVTDLGTAFHARLTGGRLVDITDGDDPKAKIALTAASDDLIALVTGKLDVTKAVASRQVSIKANPFDLLKLRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Y3</accession>
    <name>U5W4Y3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative chromosome segregation and condensation protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29640"/>
    <dbReference type="EnsemblBacteria" id="AGZ44189">
        <property type="protein sequence ID" value="AGZ44189"/>
        <property type="gene ID" value="AFR_29640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6008"/>
    <dbReference type="eggNOG" id="COG1354">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_396217_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003768">
        <property type="entry name" value="ScpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969">
        <property type="entry name" value="PTHR33969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02616">
        <property type="entry name" value="SMC_ScpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="490"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="603"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="631"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="317"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="695" mass="73212" checksum="B83DCEF90511BD93" modified="2014-01-22" version="1">MPADVEMPDAAVPEVDSGKFTVRLTNFTGPFDLLLQLIGKHKLDVTEIALHQVTDDFIAYIRAMGDDWDLGETSEFLLVAATLLDLKAARLLPAAEVEDEEDLALLEARDLLFARLLQYKAYKEAAAVIADLEGIGTRRWPRAVSLEPRYAEALPDLVLGIGAERLLKLALKQFLPKPGPPEVSIAHIHQVRVSVREHATLIRDRLRRAGSATFSLLIADCENTLEVVARFLALLELYREGLVDFEQPVSLEEMTVRWIGGDHVEGDLDIDDYEGTPAEPDTAAMDLDDLPVDPDDERRPGAVLEEEEEVDDDADLDGFEVKPIVEFEDRDQGDERRPGAAFDEDDEVDDDADLDDFEIKPIIEFEDRDRGEAGEPADPDSDSDSDSDDLESAPTARGLAVSGGVDLDAELRALETEAGSDVSDLAEATPALTDLDADVDDLDADLAEPGITDLDADDPAEVYEERPAVERDVLAAAVDELEARAELLDLHEPDTGFPDTASPAGTPADGITVVEPSFGDPDRAPAADSTDPGADLAGASDVGSVPDAVLTDAAGAGPTSLMSGLEAAEGDAVALTDAAGTGPTSLMSGPEVPEADAVADVAPTDAAGTDPTSLMSGPEADAVADAVLTDAVGADDVRETSTDGETAVGGGTDSGRADAEAPHGAEAGGIGGDEFGPDGHGQENAITASSDEEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W520</accession>
    <name>U5W520_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363650.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43060">
        <property type="protein sequence ID" value="AGZ43060"/>
        <property type="gene ID" value="AFR_23960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4853"/>
    <dbReference type="eggNOG" id="ENOG502ZFX3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_749899_0_0_11"/>
    <dbReference type="OrthoDB" id="1086776at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045756">
        <property type="entry name" value="DUF6183"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19681">
        <property type="entry name" value="DUF6183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="37016" checksum="21BE7879F28A18D5" modified="2014-01-22" version="1">MSDDDPRALAAGLAGRNDVTGLWEIAERHLHAGDAAFVADLGIAMWRRHGKDTSPPWQFRSAFDRMLRLLTLTPGTLDQALRLIAVSPDRRHARYAASLLASAHSAAELEIVLRGSAAQELRACLAHELVLRGAEAPGLGFHPLAWLPRTLTPLEGRPSLPHYHVNGGSGDVPGLSVEPFHGHGPVPPVRETTTAQDSAAIGAAVATWAEESNGRVEARIYALDADLHPDAVVDTLATLDAECLGGLKSGPGPCTATQAWEQLFLAASGGGAYPSGAFGAYGRLLAWQSVAALVGAPPDAPAAEVEALAHAGSWWSFAATTGWFDNIAWDVGLAVVSPDRRRLALLAATDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W573</accession>
    <name>U5W573_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363715.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24295"/>
    <dbReference type="EnsemblBacteria" id="AGZ43125">
        <property type="protein sequence ID" value="AGZ43125"/>
        <property type="gene ID" value="AFR_24295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4921"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_3_2_11"/>
    <dbReference type="OrthoDB" id="1864829at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="31"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="141" mass="15103" checksum="EBF3A1E8939A3E89" modified="2014-01-22" version="1">MLSELWPDKQWQGRRAPAFVVSRPAGERPRLRVSGDLDATSSAGLHDAFVAVLRQHCPPGVDLDLEGVTYLDSAGLTGLLLCRDDARQMGCDLALRNTPTMVYRILQIAGLLEDLGLARPPVPPAVPGTGGAAMGFLSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5I2</accession>
    <name>U5W5I2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_30720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560739.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30720"/>
    <dbReference type="EnsemblBacteria" id="AGZ44404">
        <property type="protein sequence ID" value="AGZ44404"/>
        <property type="gene ID" value="AFR_30720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6220"/>
    <dbReference type="eggNOG" id="COG4768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132014_0_0_11"/>
    <dbReference type="OrthoDB" id="2047474at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009293">
        <property type="entry name" value="UPF0478"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06103">
        <property type="entry name" value="DUF948"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="154" mass="16111" checksum="98CEF3C4A714DB1D" modified="2014-01-22" version="1">MDVTAGEIAGLIAAGAFLMLVLVLAVPILKLRHTVDAATQAINDLNDRTGPLLGNVTETVENVNTALGQVQVSLDGVNVQLAKVDTMTEHAQNVTASVANLATVVSAAAANPLIKVASFGYGLRKAASARRSAEDEREVRATLKQRRKAARAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5K3</accession>
    <name>U5W5K3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH-ubiquinone oxidoreductase chain 4l</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_30135</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560624.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30135"/>
    <dbReference type="EnsemblBacteria" id="AGZ44287">
        <property type="protein sequence ID" value="AGZ44287"/>
        <property type="gene ID" value="AFR_30135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6104"/>
    <dbReference type="eggNOG" id="COG1006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082058_0_2_11"/>
    <dbReference type="OrthoDB" id="2078994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="14125" checksum="F98FF4D452D1DB90" modified="2014-01-22" version="1">MTPNLLYVIVVGVLFATGVSLLLERSLTRIVMGVILLGNGANLLILLGGKAGGAPIVGATPDAEMSDPLPQIMILTAIVITLGMTAFLLALAYRSWALTGHDEVQDDVEDRRIMRLAEQDEGPGTDDTEAGAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Q6</accession>
    <name>U5W5Q6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560866.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31365"/>
    <dbReference type="EnsemblBacteria" id="AGZ44533">
        <property type="protein sequence ID" value="AGZ44533"/>
        <property type="gene ID" value="AFR_31365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6348"/>
    <dbReference type="eggNOG" id="ENOG503068I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127668_0_0_11"/>
    <dbReference type="OrthoDB" id="1746333at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="15994" checksum="9F54000FE881DF69" modified="2014-01-22" version="1">MTHDGPDSAHRRPAGLDDTTVEALGKLSEALEVVERVRGHLYSVHQLTGEADFKLDEAVELLTKAGHTELAGRISTELIGRNVVPGHWTFQLVEEFDDGYWSDFRGIEKDARDQLAGGRRHLYEAELKERRRTHGRAGHEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Q7</accession>
    <name>U5W5Q7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_24975</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363851.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24975"/>
    <dbReference type="EnsemblBacteria" id="AGZ43261">
        <property type="protein sequence ID" value="AGZ43261"/>
        <property type="gene ID" value="AFR_24975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5059"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="8"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="303"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="314"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="329" mass="35088" checksum="690493C140044C99" modified="2014-01-22" version="1">MRTLGGRYQLEQRIGIGGMSEVWRGHDQVLDRPVAVKIMAPAVEGTLGETNGVDLVRTEARSAARLAHPNVAGVHDFGTSPAPGQDVPYIVMELVDGQTLSEHLNAGMFDWRIAVRICAEVAAALAAAHGEHVVHRDIKPANIMLTPAGAKVLDFGIAAAIGSHDPDPDGPVMGTPAYVAPERFEGLPATPASDMYSLGVLLYHCLSGRLPWNAASQTELVHSQRFQDPEPLPHIEGLAPEVLDLCSRCLHKDPAERPTALVAALLLAEAVDARVYVPLVDLGAAAMQRPSVSAWDTRAAEAPTNVSLPDAHLDDAPTYDERPGRHRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5U6</accession>
    <name>U5W5U6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">L-arabinose transport ATP-binding protein araG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_25530</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364006.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25530"/>
    <dbReference type="EnsemblBacteria" id="AGZ43370">
        <property type="protein sequence ID" value="AGZ43370"/>
        <property type="gene ID" value="AFR_25530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5173"/>
    <dbReference type="eggNOG" id="COG1129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_92_3_11"/>
    <dbReference type="OrthoDB" id="551294at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="262"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="509" mass="55809" checksum="4E7B201B11CF7229" modified="2014-01-22" version="1">MSDTILQMQDITKTFPGVKALQDVNLSVRRGEIHAICGENGAGKSTLMKVLSGVYPHGSYDGEITFEGEACRFSGIRDSEERGIVIIHQELALAQQLSIAENIFLGNEQATRGLIDWNRTNHAAAQLLQRVGLHENPVTPILDLGVGKQQLVEIAKALSKEVRLLILDEPTAALNDEDSAHLLGLLRGLRDEGITCVIISHKLNEVMAIADRVTILRDGRTISTMDLATDQVTEDKIISGMVGRDLDHRFPPHESNVGEEVLRIEDWTVHSPTQHGRVVVRNANLNLRRGEIVGLAGLMGAGRTELAMSVFGRSYGTGISGRIIKDGKEIQLRSVRDAIKHGIAYATEDRKRYGLNLIEDIKRNISAAALGKLAKRGWVDENEEFKVAGGFRQSMNIKAPSVLSITGKLSGGNQQKVVLSKWIFTNPDVLILDEPTRGIDVGAKYEIYTIINRLAEEGKAILVISSELPELLGICDRIYTLSAGRITGEVPRHEATQESLMTLMTKGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W632</accession>
    <name>U5W632_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Membrane-bound lytic murein transglycosylase F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31570</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31570"/>
    <dbReference type="EnsemblBacteria" id="AGZ44574">
        <property type="protein sequence ID" value="AGZ44574"/>
        <property type="gene ID" value="AFR_31570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6390"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_18_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="4">
        <location>
            <begin position="100"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="41683" checksum="2FCEBA04CDA67F3C" modified="2014-01-22" version="1">MDDESGRGEIRKAVIRRRLPAQRRARAVRAQRTIEGLRRTFTQPDPLVRTWGGSLAPWRLVAVGVVLIMVLTFSWVRLFHIGPPSIEDLRRAAGVDEWQTLPIGVKDDQPGIAELVDGTWRGFDIDIAYMIAEDLGFRRDEVRFFGIESEDRARMEASDLQKNPVPVKMVIASYSITQEREDMPNVMFSQSYLHTEQSVITLKGHKKVASLEEFKDKDVCSLSSSTSAVAAAQSGARQLVRRNRVSECFDLLDKDEVDAVTTDAAILGGYKARFPDKYDHWDLGLDKTEPWGVSVGANEALRDLVNITLYRSFADPKDARWEQAYQANILPETAKNKNVDGLPVPLSRPQQPNTTRPRVRELPWEEVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W639</accession>
    <name>U5W639_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF305 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_25760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25760"/>
    <dbReference type="EnsemblBacteria" id="AGZ43416">
        <property type="protein sequence ID" value="AGZ43416"/>
        <property type="gene ID" value="AFR_25760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5222"/>
    <dbReference type="eggNOG" id="COG3544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074343_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005183">
        <property type="entry name" value="DUF305"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03713">
        <property type="entry name" value="DUF305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665737" description="DUF305 domain-containing protein" evidence="2">
        <location>
            <begin position="33"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="DUF305" evidence="3">
        <location>
            <begin position="46"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="193" mass="20819" checksum="6AC99082300136F4" modified="2014-01-22" version="1">MDPSGFSADGRVTVRRLFAALLLVVSLAGCSAAEPATPPPAFNATDVMFLQMSLDYIRQGDQVVATAEKRSVDPRVRALVTELRGQWQSESGTMERWLTGWQQPPSADPDAGAHAGHGDLHSLRPADLGDLEATKGADFDRTVVSLLLGHLHNCVEVSRMEATGGQYPPARKLAETMTTTRQDQIQRLLALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6I9</accession>
    <name>U5W6I9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364429.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26615"/>
    <dbReference type="EnsemblBacteria" id="AGZ43585">
        <property type="protein sequence ID" value="AGZ43585"/>
        <property type="gene ID" value="AFR_26615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5394"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_8_0_11"/>
    <dbReference type="OrthoDB" id="1775418at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="9"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="29229" checksum="C27FB46711E5D9EE" modified="2014-01-22" version="1">MTINPGAAEFPHRQIAAQLRERIRRGDWNPGERLPSIPAMAEMFGVAKQTVQRTVDQLRVEGILITKPGSGTYVRGTRRRLNRLSRGRYGAHRGYHADLAARYRQQLAGVGREPAPPEVADAFGVRDGTEMLVRRHLVRADDATVEVGASWFRVTDATGTSLERAEAFGRPLYQEAEEAIGRRYASATDTISARQPSREEAEILQIRPDTPVLHLLHVAFDETRKPIEVAQATWPGPMTTLTEEYRIPAPVPEPDPDPGLTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6N5</accession>
    <name>U5W6N5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenylate/guanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylyl cyclase class-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561000.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32045"/>
    <dbReference type="EnsemblBacteria" id="AGZ44667">
        <property type="protein sequence ID" value="AGZ44667"/>
        <property type="gene ID" value="AFR_32045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6488"/>
    <dbReference type="eggNOG" id="COG2114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1352236_0_0_11"/>
    <dbReference type="OrthoDB" id="1535749at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="2">
        <location>
            <begin position="16"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="21929" checksum="A58E797C31E4D16E" modified="2014-01-22" version="1">MNGTTAGLRCRRQTVTVLFVDIVGFTSLVDELDCSDVRDLQVDYFSVVSDRVRAAGGIVEKFIGDAVMAVFGTTGPAAGSAEEIGCAAASAVEAGLRVQEALRGRLLAGRYPVLTRVGLATGDAIVDLEETRDGGYGMVSGSVVSTASRLQAYAPHDTVVVCAATREVTDTAVAYQEMPPVSVAGKAAPVEIWRALHPQVVPAGRSLQACG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6P8</accession>
    <name>U5W6P8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364441.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26645"/>
    <dbReference type="EnsemblBacteria" id="AGZ43591">
        <property type="protein sequence ID" value="AGZ43591"/>
        <property type="gene ID" value="AFR_26645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5400"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_8_0_11"/>
    <dbReference type="OrthoDB" id="1122642at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022492">
        <property type="entry name" value="Phosphomutase_MSMEG4193_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03848">
        <property type="entry name" value="MSMEG_4193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="24290" checksum="90B5B8E43759C144" modified="2014-01-22" version="1">MATVLLLRHGRTTANASGGLAGRMPVELDETGRGQAQRVGERLRKLPLSAVVTSPLIRCRQTLELALPDIVPEVDEGLIECGYGDWEGRPLKELSQEPLWPVVQQHPSAVVFPNGEAMAAMSARAVAAIRHWDAKVTAQHGPDALWLACSHGDVIKAIVADAMGLHLDEFQRLVADPASVSVIRYTPTRPFLVRLNDTADLSALVPPPKPAEGALPESDAAVGGGAGGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W708</accession>
    <name>U5W708_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560108.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27545"/>
    <dbReference type="EnsemblBacteria" id="AGZ43770">
        <property type="protein sequence ID" value="AGZ43770"/>
        <property type="gene ID" value="AFR_27545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5583"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_2_0_11"/>
    <dbReference type="OrthoDB" id="1024978at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="458" mass="48025" checksum="BD86D69DD795ADF1" modified="2014-01-22" version="1">MLGLIAGAVDDRSARGIAAAVSRLVTAGELPVGARLPTVRDVARELGVSPTTVSEAWRSLTRAGAIQTRGRSGSFVTTPALPRQRLRYAQLGGLLQGVVRDFSTGVPDHDLLPNLTESLKRVGDARLTTSYLEGPVLPPLEAALRRRWPYQVEQLTVVDGALDALDRVTATVVRFGDHVLVENPAFPPVLDLLEAAGATVVGVPIDEHGMCPDALGEALKTYDPVAVFLQPRAHNPTGVSLTARRVSELADVLAAYPEVTIVEDDHAGDIATAPPVSLGTRLPDRTVHVAGFSKSHGPDLRLAALGGPAAVITAIADRRLLGPGWSSRLLQAVLLDLLTSADAIAQIAAARDVYAQRRAALLTALAAHGATAVSADGINLWLTVEDQQMAMLALAAHGIAVAPGAPFEVTPLGADHVRVTVGLIRDDFDSLAQILASAAGAPSRASGNRSRPHPRGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W751</accession>
    <name>U5W751_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560104.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27525"/>
    <dbReference type="EnsemblBacteria" id="AGZ43766">
        <property type="protein sequence ID" value="AGZ43766"/>
        <property type="gene ID" value="AFR_27525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5579"/>
    <dbReference type="eggNOG" id="ENOG5033260">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140987_1_0_11"/>
    <dbReference type="OrthoDB" id="1629665at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021784">
        <property type="entry name" value="DUF3349"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11829">
        <property type="entry name" value="DUF3349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="108" mass="11900" checksum="3AA06EBB8B6EF6D4" modified="2014-01-22" version="1">MSDLRSNFMMRAVEWLRAGYPTGVPRQDYVALLGVLRRKMTEEEVRKIAVDLADQSVLSGDDPISTEDIEQVISASMLQQATPEDVVRVSARLAAGGWPLVDPPDDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W827</accession>
    <name>U5W827_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_29050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the DedA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560408.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29050"/>
    <dbReference type="EnsemblBacteria" id="AGZ44071">
        <property type="protein sequence ID" value="AGZ44071"/>
        <property type="gene ID" value="AFR_29050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5886"/>
    <dbReference type="eggNOG" id="COG0586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_6_0_11"/>
    <dbReference type="OrthoDB" id="1928533at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032818">
        <property type="entry name" value="DedA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30353">
        <property type="entry name" value="PTHR30353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="23153" checksum="8024702542010FB8" modified="2014-01-22" version="1">MPFMPSFLDPQHLISTFGLIGILAIVFAESGLLIGFFLPGDSLLFTTGLLVADGTYLHQPLWLICLLVSVAAVLGDQTGYLFGRRFGPALFRRPDSRLFKQENLTRARNFFDRYGARSIVLARFVPIVRTFTPIVAGASRMRYRTFLSYNVLGGSLWACGVTILGYFLGQVAFVRSNIELILIGIVLVSVVPVAVEVLRARRRSQAGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W892</accession>
    <name>U5W892_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34855</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561563.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34855"/>
    <dbReference type="EnsemblBacteria" id="AGZ45227">
        <property type="protein sequence ID" value="AGZ45227"/>
        <property type="gene ID" value="AFR_34855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7052"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1084966_0_0_11"/>
    <dbReference type="OrthoDB" id="1204073at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="26504" checksum="945E4C0511A37540" modified="2014-01-22" version="1">MLPTPSALLLDFGGVLADAPPQDLAPPELVLRLYNLTGGVLTPGEISRSLLAGHQAYAAWRDEDHPDELSHAAVWALVTGDWPRKAQEAVRLQATRLAYDWAWRDGWALRPGIPEALRAARAAELPMAVVSNAMSGAAHRDFLDRAGIGGLFEVQIYSDEAGVRKPNPEMIWRAARELRVPAEQCWFVGDSRQRDVVCARRADAGAAILLRSPRTDKEDPAAFPEPDAFVEDGFGLRDLLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8I1</accession>
    <name>U5W8I1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_35280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561648.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35280"/>
    <dbReference type="EnsemblBacteria" id="AGZ45312">
        <property type="protein sequence ID" value="AGZ45312"/>
        <property type="gene ID" value="AFR_35280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7138"/>
    <dbReference type="eggNOG" id="ENOG5031MNQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075797_1_1_11"/>
    <dbReference type="OrthoDB" id="1899949at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016566">
        <property type="entry name" value="UCP010219"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11361">
        <property type="entry name" value="DUF3159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="28656" checksum="8A801B07D2D924B9" modified="2014-01-22" version="1">MTPGSEPRHAAHETVEERVAEEVVEGLDLKAEPEEEPLPSMSEQISEQLGGVRGLIESSVPVLAFVLLNVLLGESGLDLGKKTALYWAIGGSVGSAVAIGVYRLARKEPVRHAVNGLFGIAIGAFLAWRTGDAKNFYLPGILLTLGQVVLLIGSVAVRRPIIGYVWGVLANGGKHDWFDNSKLFRTFQWLTLVWAASLFIRAGVQGWLYLADEANAIGVVRILISWPIYAATFAFTAWAVHRVTSAQEAEAGAGAGAAAGAEPAEEAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8P1</accession>
    <name>U5W8P1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetylornithine aminotransferase</fullName>
            <shortName evidence="2">ACOAT</shortName>
            <ecNumber evidence="2">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 4" type="primary">argD</name>
        <name evidence="4" type="ORF">AFR_30230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + N(2)-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="2">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560643.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30230"/>
    <dbReference type="EnsemblBacteria" id="AGZ44306">
        <property type="protein sequence ID" value="AGZ44306"/>
        <property type="gene ID" value="AFR_30230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6121"/>
    <dbReference type="eggNOG" id="COG4992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_11"/>
    <dbReference type="OrthoDB" id="572533at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="104"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="215"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="385" mass="39396" checksum="3CF433F0E4C463B0" modified="2014-01-22" version="1">MTSFVDRWSQSMMNNYGTPSLGLISGSGSVVVGEDGKEYVDLLGGIAVNALGHAHPAIVAAVSQQVATLGHVSNLFIAEPPLALAEVLLALAGRPGRVIFSNSGAEANEAAFKLSRLTGRTHVVAADGGFHGRTMGALALTGQPGKREPFQPLPGEVTHVPYGDSEALRAAVTTDTAMVILEPIQGEMGVVVPPPGYLAAAREICTATGALLVLDEVQTGIGRTGHWFHHQSEGVEPDIITLAKGLGGGLPIGACIAFGKAGDLFGPGSHGTTFGGNPVSCAAGLAVIRTIAGEGLLDHVKRVGELIRRGVEGLNNPAIAGVRGAGLLLGIELTSPISAQLAESLREAGFLVNPIQPDVIRLAPPLILTTEQAEAFVAALSEVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Q9</accession>
    <name>U5W8Q9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560663.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30330"/>
    <dbReference type="EnsemblBacteria" id="AGZ44326">
        <property type="protein sequence ID" value="AGZ44326"/>
        <property type="gene ID" value="AFR_30330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6141"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068889_0_0_11"/>
    <dbReference type="OrthoDB" id="1003984at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="29"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="32989" checksum="A4D6DE72DDAC52C5" modified="2014-01-22" version="1">MLAPPADLIDPVAPAWGLEVASADYRPVGFGSHHWLVTETGGRRWFVTVDDLEKGRYAAGEPLDQPFARLAQALDAARAVRLPFVVAPHDPLVRLTSRYAAARYEYLDSVPGEDFGGVTRAVGVQEMLVELHGAGGGVARVEDFVVPHRDSLAAALDGREVADCGPYAAPAAQLIAAHAGRIRSVLDRYDALAAGADLGRAVLTHGEPHAANTMLTVTGWKLIDWDTALVAPPERDLWHLDGDHAVYAAATGVTPLAELLELYRLRWTIAELGEYADRFRRPHHGDANDAESWVQLTETVEETAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W964</accession>
    <name>U5W964_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycoside hydrolase family 3 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562010.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37100"/>
    <dbReference type="EnsemblBacteria" id="AGZ45674">
        <property type="protein sequence ID" value="AGZ45674"/>
        <property type="gene ID" value="AFR_37100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7506"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008392_5_3_11"/>
    <dbReference type="OrthoDB" id="949956at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_3" evidence="2">
        <location>
            <begin position="30"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="485" mass="49561" checksum="79ACCAEE94BC9928" modified="2014-01-22" version="1">MPIDPGLRRLALRTLLAAFAGKSAPDWALALLSEGLAGHTLFGANIAEPVQVAALTAQLRSVRADVLIAIDEEGGDVTRLAHRTGSPYPGNAALGAVDDPDLTRTVYAAIGTDLAAAGINLDLAPTVDVNTADDNPIIGTRSFGADPLRVARHAAAAVAGLQSTGVAACAKHFPGHGATLADSHLELPTIDAPLSVLRERDLPPFAAVIAAGTQAIMTAHIRVPELTGSGPATFSRAVLTDLLRDEYAFQGAVITDALEMKGAAGAAGGIGRAAVLALAGGADLLCIGARVDLALVEEVAAEIVAGVGDGRLSLSRLEEAAERNAAMASWTQQPVAATPHDGALGYAAARRAVRVEGSLVELTEPLVVQLESGHSIAEGRVPWGLKPHLNGTEQVSVVPAEASPEALAARAGLRPIIVVGRHTHRAEATRALIESLAASHTVAVVEMGWPSVWRPAGARAFVTTYGASHANGRAAAEALGLTAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W990</accession>
    <name>U5W990_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-N-acetylhexosaminidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31255"/>
    <dbReference type="EnsemblBacteria" id="AGZ44511">
        <property type="protein sequence ID" value="AGZ44511"/>
        <property type="gene ID" value="AFR_31255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6326"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008392_5_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_3" evidence="2">
        <location>
            <begin position="12"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="502" mass="51999" checksum="75687D847DD067F3" modified="2014-01-22" version="1">MPDWVRRRLGEGLGGVALFARNVESPAQVAALTAQLRAERPDVIVAIDEEAGDVTRFESRHGSSRPGNLGLGAIDDTDLTSAVARDLGLELAHAGITLDYAPDTDVNSNPDNPVIGVRAFGAEPGLVARHGAAWIRGLQESGVAACAKHFPGHGDTAVDSHHDVPLIDRSREWLDEVELVPFRAAIAAGVQSVMTGHLLVPAYDPDLPATLSRRILTGLLREELGFEGLIVTDGIEMQGVRRAYGLEGATVRALAAGADAICVGGDHADEQTALRLRDAIVAAVRSGELPEARLRDAAARVRKLAAWTLETQTSIGARAVADAAGSDAHEVAVIGGSVVGQAAARRAVRVTATADAPDLPLTLPPHVVEFAPPRNIAIGAETPWGVGAPLNTLLPGTTSVRLSADDLAGVTDPVAPILAGAGARPLVLVVRDVHRHPWIADALERTLAVRPDAIVVEMGVPVVVSGGLHLATYGATRACGQAAAEIIAGTSVPHLASQALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9I4</accession>
    <name>U5W9I4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative penicillin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_37140</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562018.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37140"/>
    <dbReference type="EnsemblBacteria" id="AGZ45682">
        <property type="protein sequence ID" value="AGZ45682"/>
        <property type="gene ID" value="AFR_37140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7514"/>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025328_0_0_11"/>
    <dbReference type="OrthoDB" id="423699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007887">
        <property type="entry name" value="MecA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05223">
        <property type="entry name" value="MecA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665692" evidence="2">
        <location>
            <begin position="27"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="MecA_N" evidence="5">
        <location>
            <begin position="33"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_dimer" evidence="4">
        <location>
            <begin position="155"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="3">
        <location>
            <begin position="366"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="642" mass="67060" checksum="E6CBC9687B91461D" modified="2014-01-22" version="1">MRRTALAGRGVAALASLLLMAGGVTACSSPDGPDQTLGAFLDGWRAGDLTKVGFVTADGAKISANEVLTQLQALSGDLKKSSVLLAAEGKPKETGDIASGPVKVDWTLPGGGSWSYQSTVRLTKRDSDGWRVVWEPSIVHSELTTGDALGLRRKGADRADILDADNKPIVTERDVVIVGVTPLRITDEPALLKDLGAALKTIKVDIDPKALSERIKNATDDGAFIDVVTLRRPDYNKIRNRVRALQGTVFQEAKRELAPTRQFARALLGTVDVATREDLDTNPDVLSDGDLVGHSGLQERYDRQLRGTPGQAVVISRKTPDDDVEDTQLFSTEPVAGKSIKTTLDVTTQNAADAAVAGEKQPSALVAIRISDSSVVAVSNGPEGAGVDTAMTGQVPPGSTFKMVSTLGLLDKKAVTLDGKVDCPKTKKVDGREFKNSHNMVLGSVPFRTDFAKSCNTAFVNVAPKLGADGLQDASAKLGLGAKWDLGADAFSGKISPAGSPTELAAATFGQGATAVSPLAMAGATAAVARGQFKQPKLVVDPAPASPLADGPALEASSVQPLRTMMREVVTKGTGDGLADVPGKPVSGKTGTAEFKDGEEETHAWFVGWQGDIAFAVMVQKGGAGAEAAVPIAERFLRTLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9J8</accession>
    <name>U5W9J8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative sodium/proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_31605</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560914.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31605"/>
    <dbReference type="EnsemblBacteria" id="AGZ44581">
        <property type="protein sequence ID" value="AGZ44581"/>
        <property type="gene ID" value="AFR_31605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6397"/>
    <dbReference type="eggNOG" id="COG0025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_8_2_11"/>
    <dbReference type="OrthoDB" id="1194802at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004705">
        <property type="entry name" value="Cation/H_exchanger_CPA1_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="PTHR10110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00831">
        <property type="entry name" value="a_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0050">Antiport</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0915">Sodium</keyword>
    <keyword evidence="5 6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="7">
        <location>
            <begin position="8"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="524" mass="56198" checksum="84D00D883EEED340" modified="2014-01-22" version="1">MEPIVDTVVLVAISVLGAALARRLGFIAPLVLLVAGLGLSFMPGLPEVEVKPELVLIGILPPLLYVAALQTNVVAFRRALRPILLLAVGLVVLTAFVVGFVVHALLPEVPLSACVALGAVVAPPDAVSATAIARRIGLPRRVVTILEGESLLNDASALVLVRISIGALLGSAVGFWEIAGEVALKAGGGVLLGFGFAIGAAWLHKKIVDPLLDDAVSLLTPFVVVIVAEELHTSSVVAVVVAGLYLGHRIPYLLSATSRLQMDAVWRLVTFLLEGVVFLLVGLQIRDLISKIERDLATTVTVTVAVFLTLVVVRFLWMYPATYLARLIPKVRAREERPPVTVPTVIAWAGMRGVVTLAAALTLPLNEDVANADYPRELFVFVAFAIIVLTLLIQGTSLPLLARRLGVMEDTSSEDALAEAGVQHAASRAARESLETHAEGAPQSVVDRLRALTDSRANSAWERLGNQDSETPSAAYGRLRREMITAERHVFKIARNEGRISEEVLNRAQRDLDLEESQLNRSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9R6</accession>
    <name>U5W9R6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GGDEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560989.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31980"/>
    <dbReference type="EnsemblBacteria" id="AGZ44656">
        <property type="protein sequence ID" value="AGZ44656"/>
        <property type="gene ID" value="AFR_31980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6473"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="371"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="503" mass="53546" checksum="60A681B21CA7FBF8" modified="2014-01-22" version="1">MRQILTGLRRDRLLLALCATTAGLVLWYATAPASRTAQVVTAWVFVAAVHICLIGITRSFTRTPGLPAVARRFWRAVLAGAIVYLIGDLAQIVEAASHPALATAATGGKIQVATLSAGSAWLMITLLTAPLGLTTRRERLRFWLDVATVMVAAVVVGWSLIVPDHPAALLDAAAPVLMGPVVLLLCVFVVVKLTMSGTTLFTRSCALIGTAAAALKSGADGLGPEQLHWFLALTITAHAMLTIAVRITHLQVLHNPSALETRRRRRPYSPLPYGAIVVTYGLLTVAIWRQETATIPIALAGAAISTLLVVLRQLDAFRENALLLSRLHQSLEQRDTLAARLQHQAFHDGLTGLPNRQLYSQRLDAALAADGPVVVMLIDLDDFKPVNDAYGHAAGDALLKVIADRMRACVRETDTVARIGGDEFAVLLSSLPGGDPLEIAARIVREVEAPVPVDGGVAQVGASVGIAFARPDLHDAEALLREADHAMYAVKRDGKGSYAVSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA20</accession>
    <name>U5WA20_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_38675</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562323.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38675"/>
    <dbReference type="EnsemblBacteria" id="AGZ45989">
        <property type="protein sequence ID" value="AGZ45989"/>
        <property type="gene ID" value="AFR_38675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7825"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_29_1_11"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="27"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="197"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="497" mass="55225" checksum="96C136947BA906FB" modified="2014-01-22" version="1">MRERRYRGEPGEHLVWLNFKDARHPHAGGAEEFMHQVATRLVADGQRVTVLTSRSRGTARTETIKGATVRRRGNAYTVYLYALLWLLRHRRDINGIVDTCNGIPFFAPLAVGRRVPVVVLIHHVHQSMFAQHFRWPVAQVGRWMERVGNRLVYGERTIAVVSPSSRTEVRRVLGLIGPVFVAANGQEPLAVSGAQRSEAPRIVCVGRLAPHKRWDLLIRVMPRVVELVPDVELHLIGDGPCRRELTRLVDELDLAGSVRLHGRLATPDRNRLLASGWLTVCTSEVEGWGLAVTEAMCLGLPAVVLASPGLRDSVRNRTTGWVVERPQHLAGQLTSALIELEDTAVAEAWAQRCRDWSSRFTWDATADRITGLLISEDTRRVAADGRAICDLSTVVELPVREAAKIDFTVLRSVDQVDWDAPETGAAPRVRVLLAGFDEGDATAFLSRFGIPADTLRVRVARPTDLLGWKRTGGPRGQGLRDFFQGLSAVTSTRQWTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAB4</accession>
    <name>U5WAB4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FtsX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_33385</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561272.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33385"/>
    <dbReference type="EnsemblBacteria" id="AGZ44935">
        <property type="protein sequence ID" value="AGZ44935"/>
        <property type="gene ID" value="AFR_33385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6757"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019122_0_0_11"/>
    <dbReference type="OrthoDB" id="1195369at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="613"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="666"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="701"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="642"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="737" mass="76517" checksum="6778C0DCFD4BA758" modified="2014-01-22" version="1">MIRFGLRLTLAGGREALVRLVVIAVAVALGVGMLLTALAGAGGVTTQNDRYGWANSAFAPGAEDGPDPLWWQTRIDGFDRRTVGRVDLAATGAASPVPPGLPRLPAAGEYFASPELAQLLATTPAAQLGDRFPGRLAGTIGPEALPAPDSLVAVVGYAPEVLSQRPGVSRVGKIQTVAPTSCPQGCWSGIPAAGLQLILAVVAAALIFPLLILIGSATRLAATRREQRFAAMRLVGATPRQVSVVSAVESTVSAFAGTALGFALFFLFRDTLAAIPFTGSPMFPSDLALGPVTVIGVALGVPVAAALVARFALRQVQISPLGVTRRVTPKPPRVWRVIPLLLGLGELAYFIGRRPDTTSGQLLAFLPGILVIMLGLIIAGPWLTMAGARLVARRASRPASLIAARRLADNPRAGFRTVSGLVLALFVTTVAVGVMGSIAAEQNVSREDPSAAGLFTLFRDEDRAPTGDPVPAALTSLPGVRGVAVTRVPPESVPVPHVEIGELGWPYALVSCADVARIPDARQCAPGAEVAWTWPGLDGPPGWQGTWPRADVDAAALTGFKVMAILTVTDGSTPALEEARTVLQNAHPMLYSPYSGVEFQADSARVFNGWKQLANVVILTSLAIAGCSLAVSIAGGLSDRRRPFSLLRLTGVSLGTLRRVVALESVTPLLVAAVVALGSGLLAAQLFLTAQMDSTLSPPSVSFYVIVVAGLATSLAVIASTMPLLRRITGPEAARNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAE9</accession>
    <name>U5WAE9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33315"/>
    <dbReference type="EnsemblBacteria" id="AGZ44921">
        <property type="protein sequence ID" value="AGZ44921"/>
        <property type="gene ID" value="AFR_33315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6742"/>
    <dbReference type="eggNOG" id="COG3358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090976_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012467">
        <property type="entry name" value="DUF1684"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41913">
        <property type="entry name" value="PTHR41913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07920">
        <property type="entry name" value="DUF1684"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="21611" checksum="525953369AD99CDC" modified="2014-01-22" version="1">MVGVQALELADYRAAVARIYLTATDLADFRAQRDKLFANHPQSPIPRGTAFEGINYFPPTDEFIADVDLRDAPGEIEINTDGPDGVVRYTRAGILDTPWGELSLWWLAAYGGGLFLPVRDTTCGPESYGGGRYLTDTVKGTHGRGVEMLSPTRVRLDFNYLYNPSCAYDDQWLCPLAPPENRLAVPVRAGELKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAK7</accession>
    <name>U5WAK7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative_PNPOx domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561362.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33835"/>
    <dbReference type="EnsemblBacteria" id="AGZ45025">
        <property type="protein sequence ID" value="AGZ45025"/>
        <property type="gene ID" value="AFR_33835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6849"/>
    <dbReference type="eggNOG" id="COG3467">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123922_0_0_11"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019920">
        <property type="entry name" value="F420-binding_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03618">
        <property type="entry name" value="Rv1155_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="15875" checksum="A2718BBD902B511A" modified="2014-01-22" version="1">MSALEDAWALVRDESGLAIVSTLREDTTIQSSLVNAGVLPHPSTGEPVLAFVTYGKVKLANLRARPQVTTAFRKGWQWATVEGQAELAGPSDPQPWLDPEGLRLLLREIFTAAGGSHDNWSEYDRVMAEQGRTAVLITPTRIYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAS2</accession>
    <name>U5WAS2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase is4 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_39950</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562577.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39950"/>
    <dbReference type="EnsemblBacteria" id="AGZ46244">
        <property type="protein sequence ID" value="AGZ46244"/>
        <property type="gene ID" value="AFR_39950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8083"/>
    <dbReference type="eggNOG" id="ENOG5032380">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074104_3_0_11"/>
    <dbReference type="OrthoDB" id="1180333at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027805">
        <property type="entry name" value="Transposase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13613">
        <property type="entry name" value="HTH_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_Tnp_4" evidence="4">
        <location>
            <begin position="34"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="102"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="29116" checksum="E5EB9F7E175477F7" modified="2014-01-22" version="1">MLSYPATISLSSQSLNHLATLIRHHRRQLRSRWRRLNPARQALLALAHLRNGDTYHRLATGFSISTTTAWRYVREAIDLLACAAPDLTTAMTRIRRLCFVILDGTLIPIDRVADGKAYYSGKHRRHGMNIQVVADPAGRLIWASPALPGRAHDLTAARTHGLIDALTHAGVITFADRAYQGTQSPLITPYKKHKGRRVFTAWNKQFNRDHAAIRANGERANATLKQWKVLVQVRCSPGRTTAILQAIQVLHSIEAADHPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAS5</accession>
    <name>U5WAS5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561402.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45065">
        <property type="protein sequence ID" value="AGZ45065"/>
        <property type="gene ID" value="AFR_34035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34035"/>
    <dbReference type="HOGENOM" id="CLU_2598125_0_0_11"/>
    <dbReference type="OrthoDB" id="2187465at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="79" mass="9330" checksum="1138DD924A38CB03" modified="2014-01-22" version="1">MAERIEHWFNRHYGPNRRDVYLLRTETGWQALGRQGGADGLTVTHYFDQEREARAMLHRMMATVPPELSDWAKMSQPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAW6</accession>
    <name>U5WAW6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_40545</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40545"/>
    <dbReference type="EnsemblBacteria" id="AGZ46363">
        <property type="protein sequence ID" value="AGZ46363"/>
        <property type="gene ID" value="AFR_40545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8206"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="38866" checksum="B41AB29016993881" modified="2014-01-22" version="1">MDNRTVARYTMVVIGLVLATLLLLLLAYETRQVLTWIVIAVFFAVALHPAVSWLDRKIGWMRRWLATLVVYLVAVAVIGGLIAVFVVPLVREGTQLADQLPKIIADVRAGRGPVGSLAERFHVLDYAESHAGQIRGYLSGLGASTLTVLRGAATGVAGAVTIFVLSYLMVLEAPKVVDGFLGLFSERRAERIRRVGHACARTITGYITGNLLISLICGLLTYAVLAILGVPFAGLIALFVGLADLIPLVGATLGAVVGVLAAFTDSVTAGVVVLVFFVIYQQLENHLLQPLIFSRTVRLNPLTVLLAILIGVELTGILGALLAIPVAGIIQIIARDIWDSRHGRPEPALPMSDSSGSETIRDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAY1</accession>
    <name>U5WAY1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562486.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39490"/>
    <dbReference type="EnsemblBacteria" id="AGZ46152">
        <property type="protein sequence ID" value="AGZ46152"/>
        <property type="gene ID" value="AFR_39490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7989"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_11_2_11"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="160" mass="17930" checksum="21196DFF3001CD20" modified="2014-01-22" version="1">MQAILATLGYVTSPDRRKVLMIRRDTRPDDIHYGYYNGLGGKLEPGEDVVTGMRREIREESGLESLSVELAGTISWPGFGRNGENWFGFLFRVPAWSGTPLAGNDEGSLHWMDIADVLAGKLPMWESDRHFLPLVFADEPRPFHGVMPFADGKAQSWSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB73</accession>
    <name>U5WB73_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Urea amidolyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_34845</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34845"/>
    <dbReference type="EnsemblBacteria" id="AGZ45225">
        <property type="protein sequence ID" value="AGZ45225"/>
        <property type="gene ID" value="AFR_34845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7050"/>
    <dbReference type="eggNOG" id="COG1984">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028967_0_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHS2" evidence="5">
        <location>
            <begin position="24"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="198"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="398" mass="39868" checksum="5F5E0B075AF13FA3" modified="2014-01-22" version="1">MTVTIIRPGPLTVLKDEGRPGHAAQGVAPSGAVDRTAYARANTLVGNPPGLAALECTLGGLRVRFDEPATVVLTGTDAVLSVDGARAGAEEVLTVPAGAVVSVTMPRRGLRSYLAVRGGFAVEPVLGSRSTDQLAGVGPAKPAAGDTLPIGPTPAFATTFADPTLTDPAPTDAASISPGAAGATRISSAEPASDNPRPAAPATQTGGHTGPADVSTQPPTNASGRPNILEVGHRPTDPAPQTGNHNHPADPAAQSGLGDSAAQPPTTAGPSDQLGRASDGAGSEGALEVDLGPRDDWFTGEAVETFLSAEFTVSPASDAVGLRLTGPALTRSRSAELPSEGVVRGSVQVPPDGHPIIFQADHPVTGGYPVIAVLTPEAADRAAQARPGAKVRFRVAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBC0</accession>
    <name>U5WBC0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Activator of hsp90 ATPase 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561572.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34900"/>
    <dbReference type="EnsemblBacteria" id="AGZ45236">
        <property type="protein sequence ID" value="AGZ45236"/>
        <property type="gene ID" value="AFR_34900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7063"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_6_3_11"/>
    <dbReference type="OrthoDB" id="1914312at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="146" mass="15514" checksum="8B68A826E4AE9AD9" modified="2014-01-22" version="1">MTRVELQGDELIARRWLAASPERVWAAFLTPASIAAFWGGSHATVPSESVTVDPRPGGEFALDTRGPDGSGRRLRFVYVSLDAPRELVFDEAVTGLRTTVTIRAAGEGTDLTIHQRRLPPELATAQAAGGLGSIVDALAVHLGETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBH5</accession>
    <name>U5WBH5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45340">
        <property type="protein sequence ID" value="AGZ45340"/>
        <property type="gene ID" value="AFR_35420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7167"/>
    <dbReference type="eggNOG" id="ENOG50334SM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149376_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021214">
        <property type="entry name" value="DUF2568"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10823">
        <property type="entry name" value="DUF2568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="117" mass="12317" checksum="359C94BB803E2C89" modified="2014-01-22" version="1">MRAINLLLRFVLELCALAALAYAGWQLPGAVWLRLLAAIALPLVAALVWAQWVAPKARRPVADPLRLVPEWVVFGGATVALAVTGHVILAGLLAVLAAANRLALHLLGAPTDDHAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBZ7</accession>
    <name>U5WBZ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36125"/>
    <dbReference type="EnsemblBacteria" id="AGZ45481">
        <property type="protein sequence ID" value="AGZ45481"/>
        <property type="gene ID" value="AFR_36125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7311"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022526">
        <property type="entry name" value="F420_Rv3093c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03841">
        <property type="entry name" value="F420_Rv3093c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="344" mass="37291" checksum="1B8A20B41075BFF7" modified="2014-01-22" version="1">MAMHVVDLTSMTGQDREMSTRWGMTVPLGGVPLTDHAAVFATLADAGFTDLWSSEVNGTDAFTPLALAAAWQPRVRLGSAITPVFTRGPGLLAMTAAALAEAAPGRFQLGIGASSPVVVGDWNAADFTKPYARSRDMLRFLRAALAGEVVDEEYETFRVRRFRLERPPATPPGLLLAALRPGMLRLASAEADGVILNWLAATDVSRCLSEAKSDRWNFEVVARIFVCPTEDADYARTVGRRMIAAYLTVPAYAEFHRWLGREAELGPMWKAWAAGDRKGALAAIPDEVVDELIVHGSPEQCRDHVARYAEAGVKVPVMALVPLPDQANLTPAELTDLLTRLGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC69</accession>
    <name>U5WC69_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563080.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42475"/>
    <dbReference type="TCDB" id="2.A.1.85.3">
        <property type="family name" value="the major facilitator superfamily (mfs)"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46749">
        <property type="protein sequence ID" value="AGZ46749"/>
        <property type="gene ID" value="AFR_42475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8596"/>
    <dbReference type="eggNOG" id="ENOG502Z89R">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025319_1_0_11"/>
    <dbReference type="OrthoDB" id="1885417at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="532" mass="55414" checksum="69C625DA77675D78" modified="2014-01-22" version="1">MAVGVRHFVRLKLRITANGLRGQTWRVALFVLGAVLATCAAFSGYVFLALPGLLGEERAAEVALPLAGAALTLGWLFLPLVFFGVDESLDPARFALLPLSRRALIGGLFVASLAGIPAVATLLGTLGMVHTAARLGGPFAAVVQLVGVVCGLLLCAAISRSLTSAFATALRSRRARDLATMLLAVVAALLGPLQLAALAGAQNADWGSVGRVADVVAWTPFGAAYSMGPDVVAGRIWAVPIKLVLVLATILLLLWWWSTTLERAMLGSVGRSGPRERKASAREPVAQLVPRWLPRTRFGALTAREVRYWWRETRRRASLITFGVVGVFLPVFIAVSSESAGPPTTILVFVGALAAVSLANQFGFEGSAYAANVVAGVPGRVELAARATGFSVYVLPAFLIIAAVVGIVVSEPAAVPSLIGMLLAAYGVGLAIVLPISVRVAYALPDTANPFALSSGGGMAKGLLTFGALFGAVIITVPLQLVAFLADGFWLWAGLPLGLLYGGGAVLLGLRLAGSMLDRRMPELLATVSARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCM4</accession>
    <name>U5WCM4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transketolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563053.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42340"/>
    <dbReference type="EnsemblBacteria" id="AGZ46722">
        <property type="protein sequence ID" value="AGZ46722"/>
        <property type="gene ID" value="AFR_42340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8569"/>
    <dbReference type="eggNOG" id="COG3958">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_1_11"/>
    <dbReference type="OrthoDB" id="900125at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31492" checksum="9704B65226C9FF1A" modified="2014-01-22" version="1">MRETFVATTSALLDEDPRTAVVLADISTDAFRPAQRKHPDRVLNVGIREQLMAGVAGGLALTGLRPFVHSYAPFVIDRAYEQIKLDFGHQDTGAVLVSIGASYDASAEGYTHQSSGDVALLDTLEGWTVHVPGHPDEVAAVLRAAATNDDRVYVRLSTQQNSQGYAPTSDLQVMRRGWAGAPVVLAVGPMLDPVLDATRDLDVTVAYTMTPRPLDGRGLRELVSREVVVVEPYLAGTSSRLVAAALSDVPHRELHLGVGRAEQRRYGTPADHARWHGLDPAGLRESITGFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCY0</accession>
    <name>U5WCY0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_42915</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563167.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42915"/>
    <dbReference type="EnsemblBacteria" id="AGZ46837">
        <property type="protein sequence ID" value="AGZ46837"/>
        <property type="gene ID" value="AFR_42915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8682"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021353_0_0_11"/>
    <dbReference type="OrthoDB" id="912616at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="316"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="514" mass="54733" checksum="7CD62014AA7FA3E5" modified="2014-01-22" version="1">MTQVGEGHETAADEAGPVITFGAPTVGEILAERYQLEEHVNDDSAGRQVWRGIDVILRRPVAVVLRHPGGDSATEMLQAAVDASRVIHPNLVGVYDAIDEDARAYVVREWVDGEALRELIAQEGPLDPARATSIAHSIADALAAVHATGMVHGNVHPGTTLIGDDGRVVLADARADSADSTETDVRAVGGILYFALTGHWPHTEIGRSSLPDARRDGNDAIVAPRQIRAGIPAYLDDLTMDLLDKRVATPEADALAAELARLDSADDDYEDVGPLRFAQGTSSEPVRSTRKIVIGVGALAVIAVLGLIFGIQAISNSGKDTPQNTTQANTAPQGNTSDDGSGTGTGEEPPAAAPKKIALTSDMVRIVDPPEGDRTDEGEAAYTVDDNKDTVWKTSGFRESKFYNKPGMGLLINLGSPRTVTDVRVETSAPGIAMDIRTGTSDPGNSSDGDDQIVKNYKKLGDGDTEKTDGTNAVFPVFDENQKYQYILVFITELPRDTDGKYRAEISKVEVYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDZ1</accession>
    <name>U5WDZ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562470.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39410"/>
    <dbReference type="EnsemblBacteria" id="AGZ46136">
        <property type="protein sequence ID" value="AGZ46136"/>
        <property type="gene ID" value="AFR_39410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7973"/>
    <dbReference type="eggNOG" id="COG2308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017048_2_1_11"/>
    <dbReference type="OrthoDB" id="517836at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025841">
        <property type="entry name" value="CP_ATPgrasp_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016450">
        <property type="entry name" value="UCP005522"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14403">
        <property type="entry name" value="CP_ATPgrasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005522">
        <property type="entry name" value="UCP005522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="527" mass="56725" checksum="829E274DC6A1B4C6" modified="2014-01-22" version="1">MADLFEEYRLGPGWDEMFSELGMPRETYEALHATLQPLSSAELGVRAEVLARAFLDQGITFALKGVERPFPLDIVPRIIAAAEWRKVELGVAQRIRALEAFLADVYGAGQVLADGVVPRRIIATSAHFLRAAAGINPPNGVRIHVAGVDLIRDEQGTFRVLEDNVRIPSGVSYVMENRRAMAQVLPEVFAASRILPVESYPGMLLRALRAAAPVGVSNPTVVVLSPGVYNSAYFEHALIAREMGVELVEGRDLVCVGNDVAMRTTAGEQRVDVIYRRIDDDFLDPVHFRADSVIGVAGLLNAARAGRVTIANAVGNGVADDKLLYTYVPDLIRYYLDEEPLLPNVDTYRLDEPGVLEDVLPRVRELVLKPVDGAGGAGIVIGSQATEAELAAVTEKMVADPRGWIAQREVKLSMVPTLIGNRLRGRHVDLRPFAVNDGDKIWVLPGGLTRVALPEGALVVNSSQGGGSKDTWVLADADDTVPFEVPHLPADPAPLPRAGSPDPGPAAGGGEAQQQQQQQQQQGGPPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFG8</accession>
    <name>U5WFG8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562992.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46661">
        <property type="protein sequence ID" value="AGZ46661"/>
        <property type="gene ID" value="AFR_42035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8506"/>
    <dbReference type="eggNOG" id="COG0645">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105030_2_0_11"/>
    <dbReference type="OrthoDB" id="1574819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="17513" checksum="D1B322247EEDE563" modified="2014-01-22" version="1">MADSRLIMVCGLPGAGKTTLSRELAAELGAVRFCPDEWLVDLGIDLFDIAARDRLERKLWQLTQDLLRGGLTVILEYGFWGRAEREEKRLFARELGVAVELRFLDVPLDELGRRVEARNQAGGAGAVPLTRAMIEEFLPLFEAPDAAELGLYDQPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VND1</accession>
    <name>U5VND1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00025"/>
    <dbReference type="EnsemblBacteria" id="AGZ38294">
        <property type="protein sequence ID" value="AGZ38294"/>
        <property type="gene ID" value="AFR_00025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5"/>
    <dbReference type="eggNOG" id="COG5512">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087206_0_0_11"/>
    <dbReference type="OrthoDB" id="1918294at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007922">
        <property type="entry name" value="DUF721/UPF0232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05258">
        <property type="entry name" value="DciA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="19918" checksum="783C1A92D97C65E5" modified="2014-01-22" version="1">MAEKPAEKPPAASSGGEKQDPAGPELARAVLDAALAKRRATQQRPRPRGEGGDGTGRRLRGYSGPGPDPRDPQLFGAVLERIMKQRGWQKPKAEATVFGAWEKVVGEDIAKHSRPIKLDAGVLTVEAESTAWATQLRMLAGKLLKSIAAEVGHNVVTKLNIHGPAAPSWNKGPRRVQGRGPRDTYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNX5</accession>
    <name>U5VNX5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357437.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01025"/>
    <dbReference type="EnsemblBacteria" id="AGZ38494">
        <property type="protein sequence ID" value="AGZ38494"/>
        <property type="gene ID" value="AFR_01025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.204"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_6_0_11"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="2">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="360" mass="37664" checksum="9B45890B0434A71B" modified="2014-01-22" version="1">MNAVRVGLIGAGVISDTYLKNLTSFPDLRVEMVADLDLGRAQARAAQHGVPRSGTVADLLADPGIELVVNLTVPVAHVEVGLAALGAGKHVWAEKPLALDRPSARKLLDRARELGLRVASAPDTVLGAGLQTARRAIDAGRIGEPRTALALFQSPGPESWHPAPEFLFQAGGGPLLDMGPYYLTALVHLLGPIRRVTAAGGRARETRTIGSGPRSGTEFAVTVPTTVSALLEFDRGGTAQVVFSFDSALPRTTLEITGTRGAAVLPDPNSFSGPTMLWPDQVELPAEGHQASRGTGVLELARAIRAGVPERASGALAFHVLDAMQSIDESMTAGQPISLQSKAEAPPALPADFDPYARTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPL4</accession>
    <name>U5VPL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38908">
        <property type="protein sequence ID" value="AGZ38908"/>
        <property type="gene ID" value="AFR_03095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.623"/>
    <dbReference type="eggNOG" id="ENOG5033DZ6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196126_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="51" mass="5465" checksum="7CA2DBD8F6BAA121" modified="2014-01-22" version="1">MLILGLVLLLLGIFLSVPILYTIGAILLVVGAVFWILGSVGRPVGGRKVWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR42</accession>
    <name>U5VR42_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_05770</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05770"/>
    <dbReference type="EnsemblBacteria" id="AGZ39443">
        <property type="protein sequence ID" value="AGZ39443"/>
        <property type="gene ID" value="AFR_05770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1171"/>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_18_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="220"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="287"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="548" mass="59728" checksum="7BA69EE2DA62F999" modified="2014-01-22" version="1">MGVRRGRRVSAPARRAWRRSLHLRVVTLTLVMSSLLVGGFGWFIADRSTQILLDRAEDEVNAQMQGKVEYAYQQLTVHRQVFDKTLPLTIIDTVNRLADGDSAESGGAIVSLQAERFPELKPVTSTKVDTGPLISPELVHTVADDGQVGQQIRTGDVNGVRTKYLVYGSPVPTSFGQVELYYLVPLTTEDRAANQIRTTVLVTGLALVILLGVVAGLVTRMVVTPVRVAARTAQRLSAGLLDQRMKVDGEDDLALLAASFNQMAANLQRQIVRLEEMSRLQRRFTSDVSHELRTPLTTVRMAADLLFSEREDFDPAVARSAELLQAELDRFESLLTDLLEISRFDAGFAALDAEHSDLVPIVERVADRLAGLAERVGVTIELRLPETPVIAEVDPRRIERVLRNLVGNAVEHGEGRPVEVTLATDEAAVAVTVRDHGVGLKPGEERLVFNRFWRADPSRARQTGGTGLGLSISVEDARLHGGWLEAWGEPGGGAQFRLTVPVRSGDRLVAAPLPLIPRDRAIDVLPLALEAAPVAGAEDAGEPAEVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRB8</accession>
    <name>U5VRB8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="3 5">N5-CAIR synthase</shortName>
            <ecNumber evidence="3 5">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 5">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">purK</name>
        <name evidence="7" type="ORF">AFR_05550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 5">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ATP + hydrogencarbonate = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="3 5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05550"/>
    <dbReference type="EnsemblBacteria" id="AGZ39399">
        <property type="protein sequence ID" value="AGZ39399"/>
        <property type="gene ID" value="AFR_05550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1126"/>
    <dbReference type="eggNOG" id="COG0026">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_0_2_11"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="100"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="169"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="254"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="39466" checksum="8568A9F913C534A9" modified="2014-01-22" version="1">MVGGGQLARMTHQAAIALGQSLRVLSESPDDSAALVAADVRIGTHTDLAALREFAKGCDAVTFDHEHVPTAHIAALAAEGVKIYPGAEAILYAQDKRQMRERLSALGAPVPQWRPVASAQDIADFAGSVGWPVVAKAVRGGYDGRGVWMLDDLAAAEELVGTGTELIVEERVPLRRELAALVARSPFGQVAAYPVVETVQQDGICVEVLAPAPDLPEDLAVEAQQLAIDLANALGVVGLLAVELFETEAGIVVNELAMRPHNSGHWTIEGARTSQFEQHLRAVLDYPMGETSLTAPAVVMANVLGGEPGGMSLDERLHHLFAADPGARVHLYGKQVRPGRKIGHVTVLGDTMTEVRARAVRAARWLQEGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRD0</accession>
    <name>U5VRD0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase sdr</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06195"/>
    <dbReference type="EnsemblBacteria" id="AGZ39528">
        <property type="protein sequence ID" value="AGZ39528"/>
        <property type="gene ID" value="AFR_06195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1258"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="25386" checksum="749CFBC66AC77B03" modified="2014-01-22" version="1">MVTGGGRGVGRAIVERLAAAGDDVAVIEFDPAALDWIEEHPAGRIVPVVGDASDPAVADRAVAAVPSPLSGWVNNAAVFRDAWLHEVPPGEVLALIGRNLDPVVVGSAAAVRAFLPQGAGAIVNVSSHQAQRVVRGALAYATAKAAIEGLTRALAVDYGPRGIRVNAVALGSITTERSEAHVAGLDEAGAEHFAREIRLLQPLGRMGRADEVADAVAFLLSDAAGFINGVVLPVDGGRAAQGRDPEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRG6</accession>
    <name>U5VRG6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heat shock protein 90</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_00155</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357264.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00155"/>
    <dbReference type="EnsemblBacteria" id="AGZ38320">
        <property type="protein sequence ID" value="AGZ38320"/>
        <property type="gene ID" value="AFR_00155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.32"/>
    <dbReference type="eggNOG" id="COG0326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028672_0_0_11"/>
    <dbReference type="OrthoDB" id="246194at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="PTHR11528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="HATPase_c" evidence="5">
        <location>
            <begin position="19"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="586" mass="64376" checksum="4345A311BAF43B3F" modified="2014-01-22" version="1">MSNTFQVDLRGIVDLLSHHLYASPRVYVRELLQNAVDAITAVQHDEQATAGHVSIESSDTTGDGSLRIADNGIGLTEEQVHELLATIGRSSKRDELGFARHEFLGQFGIGLLSCFLVADEIRVHTRRAGSPAVLWTGYADGRYDVKPGPEREPGTSVTLVPRRGAEMFLVGATVNQLATLYGSLLPVEVTVDGAKVTAGEVPWAAGRRASIAYAEQTLGFTPFDVIELNVPEAGLTGAAYVLPTPVNPATRGGHRVYLKRMLLAEAVEGLLPEWAFFARCVVDSTELRPTASREALYDDSLLEATREAIADQLRGWLVRLSSSDPRRLAEFLRIHHLGVKALALHDDEMLRLVEQWWPMETNTGQTTLAEFRARHGLLRYAATTDEFRQLAAVAAAQDIGLINGGYTYDAEIIERLPQLDREIKVERLEPTDLATRFEALDAATELRLRPFLSAAQRRLDRLGCEVVVRTFEPANLSALYLVSRAAHFHEEFTATREKADDLWGGVLDALADTEAPERPQLVLNHRNPLVRRVTALGDPDLTALAVEALYGQALLLGYHPIRPADAALLNTSFLGLLGRAVPGEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRU5</accession>
    <name>U5VRU5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06995"/>
    <dbReference type="EnsemblBacteria" id="AGZ39688">
        <property type="protein sequence ID" value="AGZ39688"/>
        <property type="gene ID" value="AFR_06995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1427"/>
    <dbReference type="eggNOG" id="COG2706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038716_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019405">
        <property type="entry name" value="Lactonase_7-beta_prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10282">
        <property type="entry name" value="Lactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="SSF51004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="36571" checksum="1F84810EA7D448BB" modified="2014-01-22" version="1">MGANDEYVFVGCYTGESDGEGEGVAVLRRDPATGDLTRLGVAARTPSPSFLAQHPSLPVLYAVNELDAGTVSAFAVGTDASLTPLAVRSTGGAAPCHLAVAAGAGHLLVANYSSGSVAVFPLDADGVPGERSDLLQLQGQGPVADRQEGPHAHMVFPDPNGPDVLISDLGSDRIWRARLDAVSGRLILAGPAAVAEPGTGPRHLLRSIDGALLAVGELSGTLTWFRPDPADGHLRPVGGEPTSATKPNQPSELTTGRDGRFVYVANRGPDTVSVFSWEPEGASLLTEVATGGVWPRHLTLLGDHLYVANERSQSVTVFRIDPDTGIPSSQGEPVSEGTPTCLLRWNGRQDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRZ3</accession>
    <name>U5VRZ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357385.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00760"/>
    <dbReference type="EnsemblBacteria" id="AGZ38441">
        <property type="protein sequence ID" value="AGZ38441"/>
        <property type="gene ID" value="AFR_00760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.153"/>
    <dbReference type="eggNOG" id="ENOG5033AI2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161187_0_1_11"/>
    <dbReference type="OrthoDB" id="2065970at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021426">
        <property type="entry name" value="DUF3073"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11273">
        <property type="entry name" value="DUF3073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="72" mass="8109" checksum="4C115129CD04AAAD" modified="2014-01-22" version="1">MGRGRAKAKQTKVARELKYHSPNTDLTALQRELAGAGGKSDHHFDDDNDQLVDDDDEDDADDDQDPWSPTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS08</accession>
    <name>U5VS08_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_00835</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00835"/>
    <dbReference type="EnsemblBacteria" id="AGZ38456">
        <property type="protein sequence ID" value="AGZ38456"/>
        <property type="gene ID" value="AFR_00835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.167"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_3_4_11"/>
    <dbReference type="OrthoDB" id="1864829at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="4"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="103" mass="11051" checksum="B2707F5FA58EB48A" modified="2014-01-22" version="1">MYVTEPTYQITREAAVVRLAGDFDINARDELRTTLLEAIHAAGPAEVVVDFDEAAFLDSEAMGALIEGLNAGREAGVPLRIVNAKGLVHRVLEVSGVLDLFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSH7</accession>
    <name>U5VSH7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative magnesium/manganese-dependent protein phosphatase with GAF domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07650"/>
    <dbReference type="EnsemblBacteria" id="AGZ39819">
        <property type="protein sequence ID" value="AGZ39819"/>
        <property type="gene ID" value="AFR_07650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1560"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_43_8_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="16"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="824" mass="89998" checksum="8C52607C59CB3E3D" modified="2014-01-22" version="1">MTQDLKVPWEEDPGDLYENAPCGYLTTLPDGTIVKVNATFLTWTGYERTSLVGHRRFRDLLGPGDKIYHETHYAPLLSMQDEVHEVAFDLECADGRRLPILVNSVLGRDQNGLPRVIRTTIFNATERRGYEKELLAARRAAEASERRVRVLQQIVADLAAAPTEAEVADAVVRAPGPAFGASSATVWLVDPDRDQLTAIAGTESAGLTRDDIPRDSSRAIAEVARRGDLHVIGSVEEAEEHFPELAATMLRSRRGTMVLLPLTSASADEPSVVEVLGVLGFDFTEQRELSDSELRVVRLLGHQAGQALDRARLYDDARRREIRATFLAETTRSLEVLPRLTPRARRLIERMVPEIAEWASVRLQVGPAGLLADAGGPAPDPERLTERITEVVASGEAQLTDKGPDSDCAVLPLVANGKVLGTLSLRMAPGRRTASAETVFLTDLADRAGLALENARLYEQERAIARTLQRSLLAADMPSDPRFAVETHYQAAAHDLEVGGDWFDAFMITPDKLAVVVGDVVGRGIDAATTMGQLRSAIRALATAEAGPARLLERLDRFVERVESARMATVAYAEVDLATNEMTYACAGHLPPLLTEPGGSPRYLLDARSGALGSRAGRNARLEHRIRLSAGSRLLLYTDGLIERRTRRIDAGFEVLARAYAGRRDAPLPGLTAGLADFLVGRDHADDVCLLCLSLGTEEKLERAIGADPVQIALLRKDLRGWLVSHGIDDESTQAVLLACSEAVANAIEHGYRDNPFGIVEVVARVLPESIEVCVRDQGNWPATAADVSRGRGMQLIRQVMDHVEFDRGEGTTVTMRRTRREAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSM5</accession>
    <name>U5VSM5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02130"/>
    <dbReference type="EnsemblBacteria" id="AGZ38715">
        <property type="protein sequence ID" value="AGZ38715"/>
        <property type="gene ID" value="AFR_02130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.431"/>
    <dbReference type="eggNOG" id="COG2252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024508_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904823">
        <property type="term" value="P:purine nucleobase transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="445" mass="46233" checksum="FACB05042F1DC274" modified="2014-01-22" version="1">MAYIVVLNPLILAAGVDAVGGTLPVAALAAGTALVAGVMTILMGVVARFPLALAAGLGVNALVAYEIAPEMTWADAMGLVFIEGVIIGILVLTGLRTAVFRAVPTQLKTAIGVGIGLFLMIIGLVDAGFVRRIPDAAGTTVPVELGIGGKLASWPALVFVLGVLLTLVLFVRKVKGAILIGILVTTIFAIIVEAIAKVGPSFVDGKPNPSGWSLNVPALPDKIVDLPDLSLLGKFNVFDSWSRAGWLVVLMFIFTLLITDFFDTMGTMVAVGQEGGLLDEEGMPPRTKEILLVDSIAAAAGGAASVSSNTSYIESASGVAEGARTGVANLVTGVLFLLAMFLAPLVVVVPFEAASTALVVVGFLMMTAVRQIDWTDYQIAIPAFLAITLMPFTYSISNGIGAGVISYVVIKLALGKAREVHPILYGVAVLFVLYFLRGPLESVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT70</accession>
    <name>U5VT70_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359570.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08750"/>
    <dbReference type="EnsemblBacteria" id="AGZ40039">
        <property type="protein sequence ID" value="AGZ40039"/>
        <property type="gene ID" value="AFR_08750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1780"/>
    <dbReference type="eggNOG" id="COG3214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047003_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009351">
        <property type="entry name" value="YcaQ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06224">
        <property type="entry name" value="HTH_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="330" mass="35296" checksum="B6AFFB9BE3E3C581" modified="2014-01-22" version="1">MTLEDVLRRRIERQHLGTTKARTVVDAARRIGGFHAQVASSALASATLRLPSAPDLDKALYEEKTLVRTWAARGTLHLLPAEDLPLWVAAMSTRTRETTGSWLKYHGVTAAQMTDIIAAVPDVLGAEPLTREELAGAIIGATRHEDLRGPLTQGFGAILKPLAFRGLLCSGPPRGRNVTFVAPRAWLGEWAEVPADDAIDQLALNYLETYGPAEAAEFARWFDLKPALAKKAFARIGDGPEPETPAGEDTVLLLPAFDPYVVGSLRQLDQVSSGPKSAVSRPQGWISPTLVVNGRIEGVWEPGGDGPVITAFGKLPAAVRTALPAGATII</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUL1</accession>
    <name>U5VUL1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_05630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358946.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05630"/>
    <dbReference type="EnsemblBacteria" id="AGZ39415">
        <property type="protein sequence ID" value="AGZ39415"/>
        <property type="gene ID" value="AFR_05630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1142"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_27_6_11"/>
    <dbReference type="OrthoDB" id="948440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="21"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="204"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="41107" checksum="65144F1A269D3BF7" modified="2014-01-22" version="1">MTAGSPPRVLVDATSVPADRGGVGRYVDGLLGALGAIGPDRLDLAVVAQRSDAERYSRMLVNAEVIAGPSAVAHRPARLAWEQTGLPLLAQQVGAKVLHSPFYTCPLRAGCPVTVTVHDATFFTEPEHYDNTKRTFFRSAIKTSLRRAARVIVPSKATRDELIRLLDADPTHIDVAYHGVDQTAFHAPTEEEKARVRARLGLTDAGYVAFLGAKEPRKNVPNLIRGWARAVADWPRPPALVIAGGQGHDDDIDRAVAEVPSHLRLLRPGYLRYADLPGFLGGALVACYPSFGEGFGLPILEAMACATPVLTTPRLSLPEVGGDAVAYTTEDPDRIAEDLADLLHDEPRRLTLAKAGFDRAKEFTWAGSAEVHVATWNRVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUS7</accession>
    <name>U5VUS7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_06005</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359021.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06005"/>
    <dbReference type="EnsemblBacteria" id="AGZ39490">
        <property type="protein sequence ID" value="AGZ39490"/>
        <property type="gene ID" value="AFR_06005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1220"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_9_11"/>
    <dbReference type="OrthoDB" id="1594866at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="11"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="447" mass="46608" checksum="EA74794EA5862B42" modified="2014-01-22" version="1">MTVRRLHPAWYVAAIAFIALVGAAGFRATPSVMLRPLNEEFGWSLGTISAAVSVNLLLYGLTAPFAAALMEKFGMRRVIAGALLLVSAGSGLTVFMQQSWQLILCWGVLVGLGTGSMALGFVAIVANRWFVARRGLITGVLTAGGAAGQLVFLPVLARLTDSQGWRVAALTVAAAALIVVPLVWWRLRDRPADLGVTAYGAGPDDVASLPVVAPGSAARNALRALKDAAKTRPFWLLAGGFAICGATTNGLVGTHFIPAAHDHGMTETTAASLLALVGLFDIAGTIASGWLTDRVDSRWLLGGYYALRGLSLLVLPSLFAATAHPSMVIFILFYGLDWVATVPPTVALCREYFGASGAVVFGWVFASHQFGAAIAATAAGLVRDQLGNYDAAWYGAGALALAASLLSLMLLMGKRRKDIGPAGPGIGLAFPPSHGQEAATGMTDLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV07</accession>
    <name>U5VV07_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transposase, IS605 OrfB family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AFR_12735</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40833">
        <property type="protein sequence ID" value="AGZ40833"/>
        <property type="gene ID" value="AFR_12735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2586"/>
    <dbReference type="eggNOG" id="COG0675">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021027">
        <property type="entry name" value="Transposase_put_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12323">
        <property type="entry name" value="HTH_OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="HTH_OrfB_IS605" evidence="13">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_IS605" evidence="11">
        <location>
            <begin position="175"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="12">
        <location>
            <begin position="297"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ40833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="409" mass="45829" checksum="F109C9B89F782B55" modified="2014-01-22" version="1">MQLRYNFRVYPTPSQQIELARAFGCARVVFNDGLRLRQQAREAGGKYVSDGELSRRLITEAKLTPERAWLGEVSSVVLQQALADLNTAYRNFFASISGKRKGRTLAPPRFRSRKDNRQAIRFTKNSRFKILDNSRLRLPRIGDLAVRWSRTLPSDPSSVAIIRDAAGRYFASFVVQSTDEALPPVESEVGIDLGLTHFAVLSDGTKVTAPKFLRRAARKLKRLQQALSRKQRGSQNRKKAVVKVARAHARVADTRRDWQHQLSTTIIRENQAVYVEDLCVVGLGRTRLAKSVHDAGWAGFTGMLEYKAARYGRTFGRVDRFFPSTRMCSDCGRINDKIALNVRSWDCPCGSYHDRDVNAAKNIKAAGQADFNDRRAQVRPGLVPAPRGEAVTHPDAAYSTRSVEGISVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVB2</accession>
    <name>U5VVB2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360873.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12640"/>
    <dbReference type="EnsemblBacteria" id="AGZ40814">
        <property type="protein sequence ID" value="AGZ40814"/>
        <property type="gene ID" value="AFR_12640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2567"/>
    <dbReference type="eggNOG" id="ENOG5032CI1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114908_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="12978" checksum="4C9D2D6BD6585956" modified="2014-01-22" version="1">MTRNIVGSSAIRKTAVAYLVVSVAAVVVLAVLSVSAPQQAPTTAWVRAVIVAVTSVLTVIFAHRAAAGHERALLRLRILVPIILVAVVLVLLFLPLPAWMVIEQLLCGVLLLGLAILIFRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVB7</accession>
    <name>U5VVB7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360878.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40819">
        <property type="protein sequence ID" value="AGZ40819"/>
        <property type="gene ID" value="AFR_12665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2572"/>
    <dbReference type="eggNOG" id="COG3795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2165538_0_0_11"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="23"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="110" mass="11521" checksum="AD7532EB5E4FFF5C" modified="2014-01-22" version="1">MAKFVTIGYGDRAGYDRVDPGVREAAHAHDARLRRSGADMGIAGRPVQVRNHGAAGVTTSDEPFMSSALPVAGFAIIEAATLAEAIHMVSQTPCAVAHGVVEVWPLQESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVI6</accession>
    <name>U5VVI6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_07155</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359251.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07155"/>
    <dbReference type="EnsemblBacteria" id="AGZ39720">
        <property type="protein sequence ID" value="AGZ39720"/>
        <property type="gene ID" value="AFR_07155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1460"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="145"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="23076" checksum="8FAD33333BBEA866" modified="2014-01-22" version="1">MVGEAITVLVVDGHQTFAELLGVALTGQPELRYVGHARTGEQAIRLVQELRPNVVLLDPDLPDADGIAIAELLRHRQPDTRFVILTANNESALVGRATAAGASGFLSKNGPLGDVLNAVHTAHGGGMTVSTDILARLLRSTTPVVGPRAGGLTAREHEVLVLMGSGLDPRAIARRLGISVHTCRGYVKAVLGKLGAHSQLEAVAVATRRGLLSPDRHNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVK5</accession>
    <name>U5VVK5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CMD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359271.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07255"/>
    <dbReference type="EnsemblBacteria" id="AGZ39740">
        <property type="protein sequence ID" value="AGZ39740"/>
        <property type="gene ID" value="AFR_07255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1481"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_7_1_11"/>
    <dbReference type="OrthoDB" id="1893149at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="58"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="193" mass="21014" checksum="45691F27B9B072AD" modified="2014-01-22" version="1">MSRDTRVPQAEITGVYGGLLKKFSKKMFGIVPDGLGVLWNNKPVLKVGLANGRKASKWNACDKDLKSYAHMATSSLIGCSFCLDYGYFMAHNEGLDEAKAREVPRWRVSDVFTSLEREVLEYAEAMSQTPPTVTDELSASLLRQLGAPALVELTAWVAFANQAARMNVALGIESQEFATSCGLPPLKTVASHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVR9</accession>
    <name>U5VVR9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361112.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13835"/>
    <dbReference type="EnsemblBacteria" id="AGZ41053">
        <property type="protein sequence ID" value="AGZ41053"/>
        <property type="gene ID" value="AFR_13835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2809"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_2_11"/>
    <dbReference type="OrthoDB" id="1439833at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="29"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="36611" checksum="FD734D225FC1824F" modified="2014-01-22" version="1">MTTPTLVSRTFGTARQTTHYLESGPADGPLMFFLHGWPSIGLIWRAQMEAFAADGWHCVAPDLRGFGNSSAPASHDAYTMEQVVVDLAELHDHLGGEPAIWVGHDWGSIVAGALAAHEPQRSRGVVLTSWAYFPASNSLATLVPLVDRTIYPADRYPDGQWDYYRYYTTHFETAVADLDAAPAAALASIYRSGDPSAVGQVSPMAEVARNGGRFGAAHHPPATQPDPALWPPADFDTLVEAFTARGFGPSSAWYTNDDANIAYARTAPDGGRLTQPVLFINGEWDVICTTTGNRQGDPMRAACADLVETSVPAGHWLPLERPADHIQAIRTWLSNRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVV4</accession>
    <name>U5VVV4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_13590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361063.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13590"/>
    <dbReference type="EnsemblBacteria" id="AGZ41004">
        <property type="protein sequence ID" value="AGZ41004"/>
        <property type="gene ID" value="AFR_13590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2759"/>
    <dbReference type="eggNOG" id="COG3268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_885417_0_0_11"/>
    <dbReference type="OrthoDB" id="1006830at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="358" mass="36587" checksum="78DDEFEE3FDDE2BE" modified="2014-01-22" version="1">MTDVWILGGTGRSARAIAAELRHRGVRPVLVGRDAGRLAAVADGLPTVVAGSIGETAAAIRRDRPAVVVNTIGPFTDTAAPLLDACLPSSDYLDLANDLAAVSATLGRGDSFAASGRTAVTGAGFGVTATESVVVKLCEGRPVPRRVRVDMVPSLAVEAGAVGDALAGTIVEGLPGVEGGSRFQGRRYRDGRLVPGKLAGDPIRLVLPDGSTVTTASMPLGELMAAQRASGAADVVSASSEAPSSPVVRAILPAATALLNLGPIRAFARRRLAAVQFKAAPKPREHSWGHAVIDFADGTTREGWLRVGEAQAWTGAVPAEVVRRLLDGQGKPGAYTPAALFGPGLAESCGGEYQIVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVW2</accession>
    <name>U5VVW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DnaJ-like subfamily C member 22 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_13305</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361006.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13305"/>
    <dbReference type="EnsemblBacteria" id="AGZ40947">
        <property type="protein sequence ID" value="AGZ40947"/>
        <property type="gene ID" value="AFR_13305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2701"/>
    <dbReference type="eggNOG" id="COG2314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2448054_0_0_11"/>
    <dbReference type="OrthoDB" id="1687355at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007829">
        <property type="entry name" value="TM2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05154">
        <property type="entry name" value="TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="TM2" evidence="6">
        <location>
            <begin position="27"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="106" mass="11871" checksum="A849644AF3920A2C" modified="2014-01-22" version="1">MNPSPVTLPGWKRYSVLMAEAEYESVKKDTKVAYALWCVVGIFGGHRFYLGDTARSITMLFTLGGLGLWTLADVFFVGRRVRAVNRSRRSAIMARHGIIEDLSSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVY9</accession>
    <name>U5VVY9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08005"/>
    <dbReference type="EnsemblBacteria" id="AGZ39890">
        <property type="protein sequence ID" value="AGZ39890"/>
        <property type="gene ID" value="AFR_08005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1634"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118076_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="20328" checksum="5C6E260AC67579AB" modified="2014-01-22" version="1">MLYLARHGDAENGHLSARGRRQAELLAARLADVPLSTVTHSPLPRAAETAAALPVPAVASEIVGDYPPYLPAHTPATFTPLVEGWTPDELRDGPKFAQEALSRFAVPAPEETHELVVTHSFLVAWFVRAALGAPEDRWMGLNNCNAALTVIRYTDERPPTLMLFNDMSHLPADLRWTGFPPHLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWB5</accession>
    <name>U5VWB5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH-type transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13855"/>
    <dbReference type="EnsemblBacteria" id="AGZ41057">
        <property type="protein sequence ID" value="AGZ41057"/>
        <property type="gene ID" value="AFR_13855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2813"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="29"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="272" mass="29501" checksum="0CC0C8952C7C4ADC" modified="2014-01-22" version="1">MAGMVGAPTLGTLLRGFRDRCDFALPGTVATARRAPGLRREELAMLAGVSVDYLVQLEQGRAARPSAPVVAALSRALRLDEEASALLHRAAGLAPATGSVTRVVPAGVERMVTRLPWPVAVYSADWWLLRWNPAWTALLGDSATLHGRARNVVWFEMTQPSPRVWVDPQEDEAYRDAIVGDLRVAYLEHPDDAELADLVTALHGTSPEFAERWNAARPARYLGLRKHIDHPDAGPIVLDGDVLQVPGCDLRMVVYSPAPGTPDAARLAQLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXH7</accession>
    <name>U5VXH7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Activator of Hsp90 ATPase 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16100"/>
    <dbReference type="EnsemblBacteria" id="AGZ41502">
        <property type="protein sequence ID" value="AGZ41502"/>
        <property type="gene ID" value="AFR_16100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3267"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="13843" checksum="81D527D58CAFE6E4" modified="2014-01-22" version="1">MFARWIGPDGMTTRIDHWDASTGGSWRYVSERDGTEYAFRGCFHDVRPDRIVQTFTFEGEPDGVALTTLWIDDLGDGRARLRTQSLLDSFEGRDGWLRSGMRTGLDQGYTKLERMTTDDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXI0</accession>
    <name>U5VXI0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361624.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16335"/>
    <dbReference type="EnsemblBacteria" id="AGZ41549">
        <property type="protein sequence ID" value="AGZ41549"/>
        <property type="gene ID" value="AFR_16335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3313"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_584799_0_0_11"/>
    <dbReference type="OrthoDB" id="1159993at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="467" mass="51702" checksum="85225B2637516081" modified="2014-01-22" version="1">MPSLDTELPIVLRGTGWSAEVTIAPPVHEPGDQLGESRQVYFSLPGDKVHTHGRKVLATVAYTRGRTVKFSGEASARWDDWFVKIYQCQQADVDAMRDYLHRQAVALQEANAAVPGRTRIPIEPPWAVTPVQIVRGSGEEDRAGDVATRLGAPVDALVVAERPMRTLPLWFQDHPSNSGLYLLAVSPLLIRPPWDPWCPTPAIEHLNHFASMAAGLDKLHDLGWAHCDIRPDNVCVYLIPGAREFVLVDTDSGTPTGQPPPELPTPDFYLRRPRLADGLRYDPGELRAQDRFGFALVLLTALAGRDCMERLLRTDNGERPVDSAEAVAGILRQHWRESHWQELITVLAEPFGRTGEAGRSVEDTGPWAVGWLGRLRQAVPSGAETPAPRPPGWVDPDPYADDIALFRTALRQRPHSQFELQNEANRLLERQSQRVAARTAARYAGLVLLGFAAVLIVVLFDVTGWGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXK0</accession>
    <name>U5VXK0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NLP/P60 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_16200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361597.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16200"/>
    <dbReference type="EnsemblBacteria" id="AGZ41522">
        <property type="protein sequence ID" value="AGZ41522"/>
        <property type="gene ID" value="AFR_16200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3287"/>
    <dbReference type="eggNOG" id="COG0791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1076178_0_0_11"/>
    <dbReference type="OrthoDB" id="2063919at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="SSF51110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665315" evidence="2">
        <location>
            <begin position="30"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="3">
        <location>
            <begin position="33"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="27592" checksum="C12D18D8D9598C1F" modified="2014-01-22" version="1">MRKTFKRTGLAVALVCAALAALQPTGAYAADTRDSLGVDQTLGTGQSLWSARHTYEARMQSDGNLVVYGPGGAIWSSNTRGSGARLVLQSDSNLVMYSNQGVLFSTGKARTGSNRLVMQDDGNLVLYGSSTLWASKSSSEAAIQWFYNHLGATNYEHKCELAVEHAFGTSGRYRTAAAAAGAAKDRRDHANWNNRAQRTPYTDAPRGSLVFYNTSIDGHVAISVGNGTVLSSSAPGAKIGTAPIGYFQRPYGWAWAPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXW4</accession>
    <name>U5VXW4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_17205</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17205"/>
    <dbReference type="EnsemblBacteria" id="AGZ41719">
        <property type="protein sequence ID" value="AGZ41719"/>
        <property type="gene ID" value="AFR_17205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3489"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="398"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="455" mass="48128" checksum="7FAF9DFE0E861706" modified="2014-01-22" version="1">MAGTQWARTVRQRARDRLAEAASRNAPPPFQATPDLQDTVTPVMVVQQQEPRSEDVLPRGVRTAGAWAWRFILFVVAGYLFLRVVGLLHVVIIPVVIAILLAAMFQPVSAALTKRGMKKSLAAGLVLISALLLVFGGLGLIIRTFIAQIDTLSSQVGDGIDEVQRWLSQGPLHISDAQLSQYVDRMQSALTENQGALTSGALSTATTLGEVATGFFLVLFTLFFFLRDGGQIWSFLCRLLPREARVATARAGHYSWHTLVSYVHATVLVAFVDAAGIGIGLFVLGVPLALPLSALVFLGAFIPVVGATVTGVVAILVALVANGPVTALIVLAVVIAVQQLEGHVLQPLIMGRAVALHPLAVILAIAAGIVVAGIVGGLIAVPLLAVLNTAIRYLVRHPSGEPTPGRVPPGTEPTDDDEAEVEDREQDAARASDASEPPPAETSVDEDAMPVAQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY88</accession>
    <name>U5VY88_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ricin B-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11560"/>
    <dbReference type="EnsemblBacteria" id="AGZ40601">
        <property type="protein sequence ID" value="AGZ40601"/>
        <property type="gene ID" value="AFR_11560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2356"/>
    <dbReference type="HOGENOM" id="CLU_1700466_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666203" description="Ricin B-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="31"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="154" mass="16177" checksum="24B566ACFBEA8D1A" modified="2014-01-22" version="1">MAAIALITGAGLLPAAATAAPAARTDTPAIMAGVFQIQNWGGDYECLTASPRGGAVTTAECDLDATDQLWWRTNTNEFVPWQGAIGPYYCLSASSTNVVSLPLCSGATQHVWTQPTETLRNSGNGRCLTQIANHKVATATCSGAETQRWKLFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYJ9</accession>
    <name>U5VYJ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chelated iron transport system membrane protein yfeD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_18810</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362411.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18810"/>
    <dbReference type="EnsemblBacteria" id="AGZ42038">
        <property type="protein sequence ID" value="AGZ42038"/>
        <property type="gene ID" value="AFR_18810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3821"/>
    <dbReference type="eggNOG" id="COG1108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_4_3_11"/>
    <dbReference type="OrthoDB" id="1421296at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="26462" checksum="41A1A35DD0FF4364" modified="2014-01-22" version="1">MTWALYEAVLAGALAGLIGVQVVLRRLSFFTMALTHATFPGVVAAAILGINIYLGGMLAGVLVACGVAALSRRRGQNAAAATGVILAAGFAIGVGLVATRNGFSRDLSSFLVGSILTVGPRDVVVTAVVLAVVAVVLLAGGRAFLFTGFDQAGAAAAGYRTGLVDLFLLITIEVVIVTIVPAVGTILSLALIVAPAAAARLWSDRLATVTALAVAFGVTSGLAGLLLSGRYDIAAGAAITLVATALLLVSLAATRLPRVALVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYQ2</accession>
    <name>U5VYQ2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L31 type B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">rpmE2</name>
        <name evidence="5" type="ORF">AFR_18995</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL31 family. Type B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362446.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18995"/>
    <dbReference type="EnsemblBacteria" id="AGZ42073">
        <property type="protein sequence ID" value="AGZ42073"/>
        <property type="gene ID" value="AFR_18995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3857"/>
    <dbReference type="eggNOG" id="COG0254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114306_2_2_11"/>
    <dbReference type="OrthoDB" id="2014569at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00502">
        <property type="entry name" value="Ribosomal_L31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002150">
        <property type="entry name" value="Ribosomal_L31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027493">
        <property type="entry name" value="Ribosomal_L31_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042105">
        <property type="entry name" value="Ribosomal_L31_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33280">
        <property type="entry name" value="PTHR33280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01197">
        <property type="entry name" value="Ribosomal_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01249">
        <property type="entry name" value="RIBOSOMALL31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00105">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01143">
        <property type="entry name" value="RIBOSOMAL_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="85" mass="9710" checksum="1DE4551D8B9D360F" modified="2014-01-22" version="1">MKTGIHPTYGYVVYRDRSADYSFLTRSTATSDKTVEWEDGNTYPVLDVDISSGSHPFWTGKQRLLDTAGRVEKFRAKYARARGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYZ0</accession>
    <name>U5VYZ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18975"/>
    <dbReference type="EnsemblBacteria" id="AGZ42069">
        <property type="protein sequence ID" value="AGZ42069"/>
        <property type="gene ID" value="AFR_18975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3854"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_13_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="433" mass="45478" checksum="A627904123ED3D5F" modified="2014-01-22" version="1">MTGSLWRSRSFLLLWGGQTAGEVGARISGVAVPLLAATVLHASVFQVSVLAVLGWVPYLIVSLPAGVLADRVDRRRLMIGCDLGRAVLLLALPVGALTGHLTLVLLYAVVTLTGVLTVFFTVAYRTELPGLVGPERLVDANAKVTMSQELAELAGPALAGALVGLAGAARTFFVNSVTYLISAMALALIRAPARERVAAERVGLRQAIAEGLSFVRRDAILVKLLACTAVSNFFVMATHGVDVVFLTRELGASAVQVGLVFSLGAVGGVLAGTVADRLTRWVGTARTIWVSMVVPGPLYLLIPAAQPGWGLLLFAAGFAAFGANAMLYNVSALSYRQRITPPPLLGRVNASFLWISYGSIPLGALAGGALGSAFGLRPALLIAVLGMWSASLFVVFSPLRGLRDFPAAATRSDDAPGDQRRGQQREREAGRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ42</accession>
    <name>U5VZ42_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362501.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19285"/>
    <dbReference type="EnsemblBacteria" id="AGZ42129">
        <property type="protein sequence ID" value="AGZ42129"/>
        <property type="gene ID" value="AFR_19285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3917"/>
    <dbReference type="eggNOG" id="COG2057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069088_0_0_11"/>
    <dbReference type="OrthoDB" id="1380130at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="252" mass="26451" checksum="0EC625BA6049FC28" modified="2014-01-22" version="1">MSWTADEMMTVAAARALRGDTRCFVGIGLPSTAANLARRTHAPDLVLVYESGTIGAKPDRLPLSIGDGVLAETADAVVSVPEMFNYWLQPGRIGVGFLGAAQLDRYANINTTVIGTDYDDPRVRLPGAGGAPEIAASCGEVIVVVRQSTRTFVDRVDFVTSVGHGKGPGDRERLGLRGRGPVLVITDLGVLEPDPGDCELTLTRLHPGVTAEQAVAATGWPLRIAPGVTTTEPPSDDELTALRSLTGAEEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ96</accession>
    <name>U5VZ96_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362685.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20205"/>
    <dbReference type="EnsemblBacteria" id="AGZ42313">
        <property type="protein sequence ID" value="AGZ42313"/>
        <property type="gene ID" value="AFR_20205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4098"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_705207_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="391" mass="42524" checksum="1F3FD39716097741" modified="2014-01-22" version="1">MSGGDAYRRARHLAEAGRCGEAESTLRAALATAPADADLLTLLGHVLRRQRDYAGALAASDAAVTARPDLADAHAERAESLLALVRDRDALAAAEEAVRLAPHDPWVHLVRARTLTSARRFPEARAAARHGLALAPRSVEGLLTVADVARCAGDRDEAEEAARAALALDPGNTYGRWLLAMVDAERLRVGRSMRALRDVARENPARPDVISMTWPIRSLLSALRRWFAVAIVLVGAGVLLSSWWPPAALLTRILAALFAVVIAGFALRVLIPAGRLPWRCLRLVPALLRRASLGGMVTVGVQVALLLAYAVTGWWIFAVLALVTVPVLWGLGFAELLGARLDDPGYLHALTEVGREFRDWGADLAQWWRDTKRELRETWNDDHPDTGQAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZF3</accession>
    <name>U5VZF3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-isomer specific 2-hydroxyacid dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362519.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19375"/>
    <dbReference type="EnsemblBacteria" id="AGZ42147">
        <property type="protein sequence ID" value="AGZ42147"/>
        <property type="gene ID" value="AFR_19375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3933"/>
    <dbReference type="eggNOG" id="COG0111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_0_11"/>
    <dbReference type="OrthoDB" id="1435073at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh_C" evidence="1">
        <location>
            <begin position="109"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="32440" checksum="FBB21156CE95DD38" modified="2014-01-22" version="1">MGNSRLTVSLPSASLAEALEDVEAEVLRWDMSGPAPRPEIDLVVVPYIPGSEHLRWLDGVTTRLVQAQYLGYDGVAENLPPGHVYANARGVHETSTAELALALLLASQRGIGAFVRAAGVRTPEPTWYPSLADRRVLVLGQGGVGRAIEERLTPFEVEIVRVARTAREGVHGLAELPQLLPSVEAVVVAVPLTDKSVHLVGAEFLARLPDGAVVVNVARGRVADTAAILREARTGRLRFALDVTDPEPLPVDHELFTLPNVLISPHAGGMSTAMLPRVARLVRRQIELLSAGAEPVNVVLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZI7</accession>
    <name>U5VZI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative GDSL-like lipase/acylhydrolase with Cellulose binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_19575</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362559.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19575"/>
    <dbReference type="EnsemblBacteria" id="AGZ42187">
        <property type="protein sequence ID" value="AGZ42187"/>
        <property type="gene ID" value="AFR_19575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3972"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_741091_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665907" evidence="2">
        <location>
            <begin position="28"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="3">
        <location>
            <begin position="24"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="373" mass="37939" checksum="45AC983C06CFD1EE" modified="2014-01-22" version="1">MSGKKLLACMAVAALVPIGLFAAPASAAAGCSVAYTVTNQWQGGFGASVAITNLGDPVAGWTLAFSFPSGQTVTQLWNGVVTQSGSAVSVKDAGYNGSIGTNASTSFGFNGAWSGSNTVPSSFTLNGVTCTGGVTTPPTTPPPSTPPPTTPPPTTPPPTTPPPTTPPPGAAVKVMALGDSITGSPGCWRALLWRQLQQGGYTGVDFVGTLPGQGCGFTYDGENEGHGGFLATNVADQNQLPGWLAATDPDVVMMHFGTNDVWSNRSPATILAAFTKLVGQMRASNPAMKILVAQILPMTPSNCADCNQRVLALNAAIPAWAASTSTAASPVTVVDQYTGFNTAIDTPDGVHPNDAGNVKIANRWYPALTAALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZK6</accession>
    <name>U5VZK6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PKD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13925"/>
    <dbReference type="EnsemblBacteria" id="AGZ41071">
        <property type="protein sequence ID" value="AGZ41071"/>
        <property type="gene ID" value="AFR_13925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2825"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_276310_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00801">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PKD" evidence="2">
        <location>
            <begin position="448"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1133" mass="117972" checksum="D7E66F429C06E850" modified="2014-01-22" version="1">MSSFVGSQPATAVALPSGFQEQIVFSGLSSPVDLEFAPDGRVFVAEKGGKIKVFDDLADTTPAVFADLSANVHNQWDRGLLGMTLAPNFPADPYVYVLYTYDAPPGQTAPVWNDVCANANDGRCVVTGRLSRLRAAGNTMTGSEQVLLHDWCQQFPSHSIGDITFGADGMLYVAAGDGASFSAVDYGQFPSGAPTNPCADPANEGGALRAQDIRTSGDETQLDGALLRLDPATGAAAAGNPNLGAADADTRRIVANGLRNPYRITMRPGTSEAWISDTGWNAWEEVNRVVDATAGVTNFGWPCWEGNARQSGYDSANLPICEALYTQPSGTHTAPFVAWNHSSKLVAGEACPTGSSSSTGVAFYPADGGSYPAAYNGAVFFADYSRRCIWASLPSTPGGLPNAAEVRTFVSDAASPVDLEVGPGGELYYADLGGTIRRVRYFPGNQPPSAVIDATPTQGPTPLTVTFNGAGSTDPDPADEGRLRYAWDFTDDGITDSTSPTASFTYTTAGSYTARLTVTDTLNATGTATIAVAPGNEAPTAIIDTPVATTTWKVGDTIGFSGHATDPQQGTLAASRLDWDLVMHHCSTPDTCHAHGIRSWNGVASGSFEAPDHEYPSYLELKLVATDQQGLTHAVTRRLEPRTVDLTFNSAPAGLQLAVGSSSGITPFTRTVIQGSTNSVSAPSPQNSSTFTSWSDNGAQSHVITAPTAATTYTATYAAAPVSQRIGYTQVGASLDSGDVNHMNGSRFVTGAEAATVTSMSVYSKGVQAAPNNQYQLAIYADSNGSPGARLATSTTGTLTANSWNTRPISAALTANTPYWLMYNTNGDNNMSYDAGTANQGAWSGSTAFGTWPATFGTASRWNAKFSIYATTGADTAAPTVTSTNPAAGATAVAPNAPITVRFSEAMAAGTITQANLELRGPGGTLVNRLVTYDTTQQTATITPSAALAASTPYTVTVRTGVTDAAGNALAANHQFSFTTADPSAPRFGYDRVGGSLDSGDMNFMNGSRFVNGSTALTISQISVYAKSVQASPGNQYQVAVYTDADGSPGTLVASSTSGSLTANAWNSRPVAATLAPNTAYWLVYNTNGDNNLSYDTGSANQGAWSQSRPFGTWPASFGSSSRWTAKFSIYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5W004</accession>
    <name>U5W004_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_20425</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362729.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20425"/>
    <dbReference type="EnsemblBacteria" id="AGZ42357">
        <property type="protein sequence ID" value="AGZ42357"/>
        <property type="gene ID" value="AFR_20425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4142"/>
    <dbReference type="eggNOG" id="COG1135">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_3_11"/>
    <dbReference type="OrthoDB" id="1058434at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033232">
        <property type="term" value="F:ABC-type D-methionine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03258">
        <property type="entry name" value="ABC_MetN_methionine_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026253">
        <property type="entry name" value="ABC_MetN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041701">
        <property type="entry name" value="MetN_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018449">
        <property type="entry name" value="NIL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43166:SF5">
        <property type="entry name" value="PTHR43166:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09383">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00930">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="2"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="328" mass="35028" checksum="6C4D5F06E9D13DF8" modified="2014-01-22" version="1">MIRIEGLRKTYHSRGRDVVAVDGVDLTVPQGEIFGVLGQSGAGKSTLLRCVNLLERPDAGTVVVDGVDLTALREKQLRAARRRVGMVHQHFALLASRTAAGNVAFPLEVTGMSRADRARRVAELLDLVGLGDRASAYPAQLSGGQKQRVGIARALAAGPQVLLSDEATSALDPETTRSILDLLLDLNRRLGLTILLITHEMDVVKRVCHSAAVMRDGRFVESGQVAELLRRPDSELARGLFPLPPAEPRPGETVVDVTLSGDTADEPILAGLARRFDLDVRILNGAVETLAATRAGRLRLALPGSAAETAPALAHLRERGVLVTEAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W063</accession>
    <name>U5W063_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyoxalase_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21260"/>
    <dbReference type="EnsemblBacteria" id="AGZ42524">
        <property type="protein sequence ID" value="AGZ42524"/>
        <property type="gene ID" value="AFR_21260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4312"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108054_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase_6" evidence="2">
        <location>
            <begin position="56"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="19582" checksum="335FDD8D92390C73" modified="2014-01-22" version="1">MSAGAGFVDDGAAGEGGSSEYEQVHGPTLPFVVPGAEDVIADSGNGVPGMALRVDLTFDCVRAEELAEFWKLALGYVDEPPPAPFRTRDEWVASFGPPGEDEGGGAWLHDPEGVGPRLVFLEVPEPKVAKNRLHIDVRVGKYGEAWGRVEQKVAQLVGAGASVRAVVGTHHVVLADPEGNEFCVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W075</accession>
    <name>U5W075_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="4 14">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">Bacitracin resistance protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 14">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">uppP</name>
        <name evidence="15" type="ORF">AFR_21310</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the UppP family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="4 14"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362906.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21310"/>
    <dbReference type="EnsemblBacteria" id="AGZ42534">
        <property type="protein sequence ID" value="AGZ42534"/>
        <property type="gene ID" value="AFR_21310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4323"/>
    <dbReference type="eggNOG" id="COG1968">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060296_1_0_11"/>
    <dbReference type="OrthoDB" id="1826154at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="PTHR30622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00753">
        <property type="entry name" value="undec_PP_bacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="6 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0133">Cell shape</keyword>
    <keyword evidence="14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ42534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="30528" checksum="60EE41E760639CD7" modified="2014-01-22" version="1">MNIFEAVLLGAVEGFTEFLPISSTGHLTIVEKLLGYQIDAGDITAFTAIIQSGAVIATVIFLRKDIARIISGWVRGVLNKDQREDPDYKFGWAVILGSIPIGIVGLLFQDTIESTLRSLWFVAGALILWSGVMAFADHAATQVRHEEDVTWKDTLIIGIVQCLSLIPGVSRSGATMSAGLLRDLDRVTVTKLSFFLSIPALLAASVLQTVTEYDNISADNGGVGWLPTIVATIVSFIVGWFAVSWLLKFIARHSYSIFIGYRLVLGSVLMVLLATNVIEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0J5</accession>
    <name>U5W0J5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative tricarboxylic transport membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15870"/>
    <dbReference type="EnsemblBacteria" id="AGZ41456">
        <property type="protein sequence ID" value="AGZ41456"/>
        <property type="gene ID" value="AFR_15870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3222"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="32339" checksum="C4117B52092E4BD7" modified="2014-01-22" version="1">MLAGALGLTLGGCAAPEADLRLMVPNPPGSGYDITARVLARALDASRIARGVEVFNLPGGSGVVGLRRLAYEHGNNHLVMLMGLGLVGAELAAGPPMILADATPVARLVQEPAIVVVRRDSPFGSLDELIEGWRADTGLRAGGGSAVGGPDHLATMLFAEAVGLSPARVAYTRHDGGGDLLAAILGREVDFALSGLSEYADQIESGQLRALAVTSEGPVPRVAVPTVRSAGYDIVVNNWRGMVAPPGLSAPDTEELRTLIGRLATSTEWAGARARFGWTDAYLAGDAFATFMADESRRVAGVLDRLGVTARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0L4</accession>
    <name>U5W0L4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16240"/>
    <dbReference type="EnsemblBacteria" id="AGZ41530">
        <property type="protein sequence ID" value="AGZ41530"/>
        <property type="gene ID" value="AFR_16240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3295"/>
    <dbReference type="eggNOG" id="ENOG502ZA20">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013425_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008757">
        <property type="entry name" value="Peptidase_M6-like_domain"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03296">
        <property type="entry name" value="M6dom_TIGR03296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665415" evidence="1">
        <location>
            <begin position="20"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="671" mass="72230" checksum="405A390B6DCAE233" modified="2014-01-22" version="1">MTAVLPVLLLALAATPAGAAPPSGDGPANPWQVRHWPQTQPWQQAQPAARTFAAGGPRAVDPQNYELPDTMTWEDYRPTPGTDWSDPSVPGSVENFKGALVLVDFPTQPFVVTQPAASTIYGNPSGVHDLARADVPGFYRDFLNKPGELNRGHTIHEYWMEDSGGRYGIELGSFGVYQMPAEGYEYGIESSMQRGMGCPAGHTCGRDLRRDARAAWIADVGEDVAAGYDFIFFLAAGQDESATWQEFGQMKFTTKEDVSDAFGPPDELLPNWNNTRYVDWTSWAASSAIWPNATNGSSLQAESSGMSTYAHEFSHILGVGDNYNNPYSVPARRDYSGPWEMLSRGTFNGPGGPHSRWLIPGTAGSSMGAQHMLRNKMKLGIIADDKVLRLSREDLATSGVVVARLTARAVEPGARGLSGINVTLDGGDRSPACTTATDPFCDGGGYDNYTVEVVDRMGFDSFTPDAGVLLAKTKDEDAAPFIWTVDANPQDIDKVDFVRPDGTPQKMTIGDYRQLSDALFHAGTDSGSEYEYVDTANRLHFYVLDLQRDRRGVLSYTVAVRSLDGAGPQARDVTALPALAAAGSSGWSRCRIPVRNTGGGPDVLRIATGAVEGWTSTLPRAVIDVAAGRTKLVDVYVQHTGGAARTAKIPVTVTSESDPSVNATTSCRVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0P5</accession>
    <name>U5W0P5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363065.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22120"/>
    <dbReference type="EnsemblBacteria" id="AGZ42694">
        <property type="protein sequence ID" value="AGZ42694"/>
        <property type="gene ID" value="AFR_22120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4484"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043786_0_0_11"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="150"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="30381" checksum="2497D62887527F28" modified="2014-01-22" version="1">MKITALRETGSSSSSYRLAWVAATGDGSPAATAYLRVFTVAGAGHRAEAEIYVHPAERRRGVGGTLLDTVVAAGRELGKRTLIAGPVDAGSAGESFLTARGLRPVLSLTYTRLALTDDPPQVATPAGYRLISWEGTVPDELAATFAESRRAMDDMPMDDADFGVETWDVDRVRAIAAAIKERGDILCTVAALDESDGTIAGFTELVVPGSGTGDAQHYGTGVLPAHRGHGLARWMKIEAIRHTRTRHPGITGLLADTADSNTVMRKINDSLGYAPTHRSVLFQLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Z3</accession>
    <name>U5W0Z3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">UbiA prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_16370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361631.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16370"/>
    <dbReference type="EnsemblBacteria" id="AGZ41556">
        <property type="protein sequence ID" value="AGZ41556"/>
        <property type="gene ID" value="AFR_16370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3320"/>
    <dbReference type="eggNOG" id="COG0382">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075132_0_0_11"/>
    <dbReference type="OrthoDB" id="1472516at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="103"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30890" checksum="DAB19C019D75A92A" modified="2014-01-22" version="1">MTTVRDRPAALLLAVSRPWFWPVSWVPAYLGTVLAGHAWLPARADTPRALVALLVLGPLIWGAVLAQNDLYDLSSDRANPRKATAPLVTGAVSAARLRSWYRVIAAASLACALFVGPLFVLGVLGVLVLGWAYSVPPLRLKTRPGWDVVVNAVVVGVVSPAAGWSITRAPWDFPWQFGLIGLLFAAALYLPTTVTDLAADTGAGDTTFAVRFGSRLAYVIGIVLWGAALAISLVCASLDVLVPRSTLAAQLVMVPVLLAAYAILTLRPTITKLALLSVVFGVPTAGFALAYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1A5</accession>
    <name>U5W1A5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23270"/>
    <dbReference type="EnsemblBacteria" id="AGZ42924">
        <property type="protein sequence ID" value="AGZ42924"/>
        <property type="gene ID" value="AFR_23270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4716"/>
    <dbReference type="eggNOG" id="ENOG5031TKJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745753_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="149" mass="15963" checksum="164955D861BDCA4F" modified="2014-01-22" version="1">MVLRGYEPAAVDALLDQAEAATASADESVQAAARGALQDASFAVALRGYDRLQVDEEVGRLRAALGDGAVSHTPAEPQPDDFTVVLRGYDRGEVDRALVAADAALETDGEFARAAARDALRDTRFAVTWRGYDRGQVDRSVTARLQRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1D5</accession>
    <name>U5W1D5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_17190</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17190"/>
    <dbReference type="EnsemblBacteria" id="AGZ41716">
        <property type="protein sequence ID" value="AGZ41716"/>
        <property type="gene ID" value="AFR_17190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3486"/>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029843_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006189">
        <property type="entry name" value="CHASE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042240">
        <property type="entry name" value="CHASE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03924">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01079">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50839">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="CHASE" evidence="8">
        <location>
            <begin position="127"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="358"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ41716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="589" mass="62900" checksum="0FFDC3E7413FC3F1" modified="2014-01-22" version="1">MIALGLAGTALAAFALRRTDADRLSRALDQQTAIVAQTLAGEMRRYTTSLLDLAAAVGSQSRLETAEFAAITAATDRQRLPGASGVSLVVPATTGQIGGVERAWRTAGSPGLRLRAAPGIRDGHLFVVQSRPIDGTAEAVGQDLAASTPAATALAKARDSHQVAISPTYRLLEDGDVPSARRQWSFVLAAPVYSTSPAAPDAGQFRGWMVMGLRGADFLHQAISRTAGNTVVLALSDSSAGRRTEVGRWQPAAEPARGQPARTVPIVVPQGTWELTVTPTDRLLPGTDLHLDMIAWAVGGFITLLLAALTGTVITARNRALRRVDAATAALRDDIDRREAVEVQLRQRESELVGFAGIVAHDLRSPLARITGYADFLREEAAPRLEPLHRDFLERLYGGAQRMQSLIDDLLDYATADNQDLVTAQVDLNRMVAEVVRDRVSGHGAQQPRIVVWPLPTVDGDATLLRQVVDNLIGNALKYTPEGEDPSIEITSRREEAGGWRVEVADRGIGIPPDQLDTIFDAFIRAGGSEGYHGTGLGLAIVHRIIERHGGLVGVDSNPGGGSRFWFSLPAHARLVAGTGATERIVHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1F0</accession>
    <name>U5W1F0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Family 1 extracellular solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17610"/>
    <dbReference type="EnsemblBacteria" id="AGZ41800">
        <property type="protein sequence ID" value="AGZ41800"/>
        <property type="gene ID" value="AFR_17610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3569"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035417_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="558" mass="60155" checksum="0592050559969C7D" modified="2014-01-22" version="1">MSSPAGPSADRRTFLSLIGLSAAAVAGGGVLGGCADKATRGGATQQLDKLAGILPEQRALTIAVPKPDIISTRPVADGYTKFPARLVDAITDKPGAGGPAIRAMVPVWGPAPPSGSSNAYLTAINTELGTEVDFSVQDGLKYAEKLGAILGARDVPDVLCVPGWEVSKLARFGDAVGALFEDLTPYLQGAAVGAYPMLGTFPAGAWRNACWNERLMAVPNPTDGPFPWGLFHRKDLLTKAGLAAPTTIDELFEVGQKVTDADKGVWAFSDIFAMVQMFHKVPGSKDGWRLKADGTPEFKYETAEFKAAAEFMAKVYQAGLVHPDIVASKGADAKQLLQSGKIMFHQDGIGMWQVMQAEQQKITPGFDVQPVKLFSATGGSPLAWGDDEPISYTFVKKGLGKARVEEILRIVNWCSAPFGTSEFDLREYGVEGKHHTRSADGPVKTELGFKEIQNQYFFISGRSPVVQPYPQTPQYVPQLLAYSNETVKHLEKDPWDGLKFEMPNKYKAGLVPTEDKITDIVRGRRPLTDLDTVVKEWRAAGGDEARQLLGTALTDAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1F4</accession>
    <name>U5W1F4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362083.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17635"/>
    <dbReference type="EnsemblBacteria" id="AGZ41805">
        <property type="protein sequence ID" value="AGZ41805"/>
        <property type="gene ID" value="AFR_17635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3575"/>
    <dbReference type="HOGENOM" id="CLU_177490_0_0_11"/>
    <dbReference type="OrthoDB" id="2220570at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="105" mass="10807" checksum="D7E98DE3D2CF485F" modified="2014-01-22" version="1">MAFATAVPAIDRTGSYGLARPDLAEAQESLRQVHGAAADDLWTALTTSAGLTGTETDSASFGRLIEAMLAADPVTALCGRALIIRGSAYVYLADAPPSDTPVAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1T0</accession>
    <name>U5W1T0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_23595</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363577.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23595"/>
    <dbReference type="EnsemblBacteria" id="AGZ42987">
        <property type="protein sequence ID" value="AGZ42987"/>
        <property type="gene ID" value="AFR_23595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4781"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665999" evidence="2">
        <location>
            <begin position="33"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="3">
        <location>
            <begin position="89"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="29291" checksum="70CC1458FFB83C6D" modified="2014-01-22" version="1">MPGRPTDRPILTRRRLLTALPGAAGLTLLAGAASTATIGAGSLAGHRMTTQPRTPPTESATAPMRAQHHPVHTLADFRRATTVPPFPGDAIALTIDDGPHPVWTPKILRLLDKHHVPALFCMIGNQVLGHEPVAKSVIRAGHQLANHTWSHPSALAGKSRARIRKEIHRAQEKIHDTTGYTPKIFRSPGGDWSPAVLQESAHAGLLPLDWSTDPRDWSRPGTAAIKRRMLAARPGQILLCHDGGGDRSQTYQALRTIIPALKARGLHFVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1X8</accession>
    <name>U5W1X8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362363.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18560"/>
    <dbReference type="EnsemblBacteria" id="AGZ41990">
        <property type="protein sequence ID" value="AGZ41990"/>
        <property type="gene ID" value="AFR_18560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3767"/>
    <dbReference type="eggNOG" id="COG2021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_1_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="28775" checksum="DC6BEC6C5C125240" modified="2014-01-22" version="1">MPLAAVNGIQLHYKEFGSGDPVVLIQGTGGGHSVWLLHQVPDLTAAGYRVIVFDNRGIPPTSVCAEGFTIDDLAGDVAGLIEHLRLGPSHVVGTSMGSFVAQELALSRPDLVRSAVLMATRGRSDRLRAELTRAEIELHDAGITLPPRYAAVVRALKSLSPRTLDDERKVADWLELFEFSPGAGAGQRAQMEISKLRNRLPAYAGITVPCQVIAFADDLITPPHLGREVADAITGARYELIADCGHYGYLEDPESVGKCIVDFLAAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Y8</accession>
    <name>U5W1Y8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MFS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18610</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18610"/>
    <dbReference type="EnsemblBacteria" id="AGZ42000">
        <property type="protein sequence ID" value="AGZ42000"/>
        <property type="gene ID" value="AFR_18610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3777"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046685_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="21"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="42097" checksum="AB1DAB3E5424BE06" modified="2014-01-22" version="1">MSARPSLLLRARSLFREPPADRTVRLLGWFNFFGDFRLYGPIMVIYFAQVSGSYTAAGSLLAVKMLSSAAFEVPAGILSDRLGRRGTMIAGAVVMVAAHLGYAAAPGYGLLLAAVVLEGLATALWSGNNEALLYDTLLEAGRADEFPRHSGRVNSMFQIALALGAAVGGVVAGASSLRTVVVLSVVPQVLCVLVALRIREPRVHGPLESSVLSHLGSAVRGIRRSPVLRRMTLVSALRYSSESAAQLSPVFVAGLWPFWALGLWRTFGHGVAFAGFRASGWVIGRFGAVRTLRGGELFDNVVNFVALVKPTVVSPVLLGSPAYGMSTIAQQTLLQREFSDRERATMGSLAALLGSVLYALVAVGAGLVADRWGIVAALFTIQAVTLIALPLAWRAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W215</accession>
    <name>U5W215_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25035"/>
    <dbReference type="EnsemblBacteria" id="AGZ43273">
        <property type="protein sequence ID" value="AGZ43273"/>
        <property type="gene ID" value="AFR_25035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5072"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030024_5_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="62"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="28128" checksum="6C7759172A384DDE" modified="2014-01-22" version="1">MTVTAMPALMYHSVSAVGGPLSDLAVPRGRLAEQLGALTAAGYRLVGLSEALDLLDAGCTDKLVAVTFDDGYRDFLTDGVPVLAEAGAGATLYASVGHLGERAGWLGRWAPDFGRLLTWDELADVAAAGIEIGNHSLLHHPLDVLPADRLREETVRSKDELEQRLQQRVRSFAYPHGYNGGKVRAAVLAAGHDNATEVGRRLHTPGERRFAVPRLQPTPGMTGADLVDLVAGGGSPVVPQLKRLAQPGWRVVRRVARAAGRNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2C0</accession>
    <name>U5W2C0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anti-sigma-factor antagonist</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362492.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19240"/>
    <dbReference type="EnsemblBacteria" id="AGZ42120">
        <property type="protein sequence ID" value="AGZ42120"/>
        <property type="gene ID" value="AFR_19240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3908"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2285366_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="12"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="101" mass="11669" checksum="A327229388386F33" modified="2014-01-22" version="1">MRSDHTIREATVVVTEALDGEAVERWRDRFTEAAARRPERLVVDLRHSPRIDAAAIVLLLQLHREMLRADGQLTLRAPVERVRRMLGLARVDHVLEVEESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2J5</accession>
    <name>U5W2J5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-(Acyl-carrier-protein) synthase III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362572.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19640"/>
    <dbReference type="EnsemblBacteria" id="AGZ42200">
        <property type="protein sequence ID" value="AGZ42200"/>
        <property type="gene ID" value="AFR_19640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3986"/>
    <dbReference type="eggNOG" id="COG0332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_3_1_11"/>
    <dbReference type="OrthoDB" id="872193at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACP_syn_III" evidence="2">
        <location>
            <begin position="111"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="ACP_syn_III_C" evidence="1">
        <location>
            <begin position="241"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="333" mass="34902" checksum="33FD1E48E4BBC881" modified="2014-01-22" version="1">MQSVPRVGILGTGSYVPDLEVTNDNLVERVTDTTSEWITRKTLIEARRFAAPDEATSDLAVKAAVAALDRAGISAERVDYLIVSTSTGDSPQPPTASLVQHAIGAYRAAAFDINVVCAGFVFGLALANSLVATRPDAVVLVVAADVYSRILDFDDRRTAILFGDGAGAAVVGAVPAPYGLVDLELVTRGDAHELIHVKAGGSRLPASAETVADGDHFFRMNGRGVRDFVMAGVPPVLADLLKRAGFTAEDVKHFVPHQANGVMIEELVEAAGLGSARTHLVLDRYGNTGSASAAIALDVAARSGELHDEELVLIAGFGGGMSVGAALLRWYER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2S9</accession>
    <name>U5W2S9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362662.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20090"/>
    <dbReference type="EnsemblBacteria" id="AGZ42290">
        <property type="protein sequence ID" value="AGZ42290"/>
        <property type="gene ID" value="AFR_20090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4075"/>
    <dbReference type="eggNOG" id="COG4572">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129869_0_0_11"/>
    <dbReference type="OrthoDB" id="1882650at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1740.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009317">
        <property type="entry name" value="ChaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037205">
        <property type="entry name" value="ChaB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011112">
        <property type="entry name" value="Rho_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06150">
        <property type="entry name" value="ChaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07498">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140376">
        <property type="entry name" value="SSF140376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho_N" evidence="2">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="15020" checksum="6EF763DC16AF09A3" modified="2014-01-22" version="1">MPARENLPSTIARSPQKAQDTYAETLDSAIAQYGDGERAHRTALASLKHSFEKVGDHWEPKEQRGPSDEQAERGAADTALPTAGGVDANASKQHLLEVAKRLDVRGRSRMTKDELVEAIGKANDAESRRTLKAEREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3T6</accession>
    <name>U5W3T6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Maltokinase</fullName>
            <ecNumber evidence="5">2.7.1.175</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Maltose-1-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_27105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-maltose = ADP + alpha-maltose 1-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31915"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17306"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.175"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aminoglycoside phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.175" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560020.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27105"/>
    <dbReference type="EnsemblBacteria" id="AGZ43682">
        <property type="protein sequence ID" value="AGZ43682"/>
        <property type="gene ID" value="AFR_27105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5493"/>
    <dbReference type="eggNOG" id="COG3281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029675_0_0_11"/>
    <dbReference type="OrthoDB" id="268166at2"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040999">
        <property type="entry name" value="Mak_N_cap"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18085">
        <property type="entry name" value="Mak_N_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Mak_N_cap" evidence="8">
        <location>
            <begin position="8"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="436" mass="47483" checksum="96E9FB7AD3670ACD" modified="2014-01-22" version="1">MTLPYAEWLPQQRWYAGRSRTLDDVKQASSTPLKDDLDLVLLDVSYTDGSSERYQVLVAWDSGPISEYSSVATIGTDGDRTGYDGLYDPAAATYLLSLVSSDATVGDIVFQSEPDATFPLEHASRVFDAEQSNTSVIFEQEAILKVFRRVDRGISPDIELNRVLGRAGNPHVAKLLGSFETTQDGEPYPLGMVTAYALNSAEGWAMATASARDLFADAELGADEVGGDFAGESYRLGEAVASVHQTLAAELGSGPAPFPVDSVIARLASATAAVPELATYGPAIEESFRKLTGEQITVQRVHGDLHLGQVLRVPAAWLLIDFEGEPGQPLDERRQPDSPLRDVAGVLRSYEYAAYQLLVDQDDDEHLAGRAREWVDRNRDAFCDGYAAASGHDPREQGALLAAYELDKAVYEAAYESRHRPGWLRIPLRSIARLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Y2</accession>
    <name>U5W3Y2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_27355</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560070.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27355"/>
    <dbReference type="EnsemblBacteria" id="AGZ43732">
        <property type="protein sequence ID" value="AGZ43732"/>
        <property type="gene ID" value="AFR_27355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5545"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="337" mass="36310" checksum="05D89B8D2B6EBE24" modified="2014-01-22" version="1">MVTSRDVARLAGVSQSTVSYVMSGRRSISPETRRRVEAAIEELAFQPNAGARALKSQRTQVIGLVVPFGPGADTSGLLPFIETIANAAREQDHDVLLVTRDEGSAGLTRLASRSLCDAIVLLDIEARDARIPVAAALTVPVILIGLPEEPLGLPCVDMDFELAGKLAVDHLAANHDRIVVIGHPPDIITRDLNFIRRFRSGTDAAARAHGLPYELHELPERGPAGVIAALDAAGRPRRGERLGLVVPNTEAVQPVLNILTERKLIPGHDVSVVGLCTDVLAESTTPPVTNVSLEPRDVSRRAMRTLFRLLDRDSSVPIDPVDLISPRLTRRQTTLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W406</accession>
    <name>U5W406_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28435"/>
    <dbReference type="EnsemblBacteria" id="AGZ43948">
        <property type="protein sequence ID" value="AGZ43948"/>
        <property type="gene ID" value="AFR_28435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5765"/>
    <dbReference type="eggNOG" id="COG3861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140819_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030077">
        <property type="term" value="C:plasma membrane light-harvesting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045156">
        <property type="term" value="F:electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019684">
        <property type="term" value="P:photosynthesis, light reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014747">
        <property type="entry name" value="Bac_photo_RC_H_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="17"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="14008" checksum="BF8E170EE571054C" modified="2014-01-22" version="1">MRTHGHGHPGDMFPAENLRDWRGEKVIDPDGDKIGSLEAIYFDTATDEPAFVTVQIGIIGRHRLAFVPLAGATVTPSAVRVRFDKKLVGDAPTIDLDGELTAADEPAVFAHYNLPYATGAAGERRLARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W473</accession>
    <name>U5W473_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alpha-galactosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_22550</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="6">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363199.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22550"/>
    <dbReference type="EnsemblBacteria" id="AGZ42780">
        <property type="protein sequence ID" value="AGZ42780"/>
        <property type="gene ID" value="AFR_22550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4571"/>
    <dbReference type="eggNOG" id="COG1486">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045951_1_1_11"/>
    <dbReference type="OrthoDB" id="277080at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001088">
        <property type="entry name" value="Glyco_hydro_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022616">
        <property type="entry name" value="Glyco_hydro_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32092">
        <property type="entry name" value="PTHR32092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02056">
        <property type="entry name" value="Glyco_hydro_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11975">
        <property type="entry name" value="Glyco_hydro_4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00732">
        <property type="entry name" value="GLHYDRLASE4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_4C" evidence="7">
        <location>
            <begin position="195"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="430" mass="47715" checksum="8C5ECDFE4114178C" modified="2014-01-22" version="1">MTTIAFLGAGSVVFTRELLADILTFDELRGVTLALHDIDPERLETAEAIARRTAEQLGAKPTITTSLDRRTVLDGADFVINAIQVGMHAATVRDFEIPAKYGLRQTIGDTLGVGGIFRALRTFPVLAGIAADMRELCPDAWFLNYTNPMAMNIGYLAAIAPDLKAVGLCHSVYWTVRDLSDLLGVPYDEVDYTGAGVNHQAWLLRFEHRGENLYPRLDALLERDPELRRRVRMDMYKRLGWFPTETSEHSSEYLPWYLHDDAEIERLRIPVGDYLRISADNVTEYHETRRAVLAGEPLDVSRDATEYAPQVIHSITTGTLRRIHGNVANRGLISNLPEDYAVEVPCVVDALGVRPERVGALPAQCAALNRSFVNVGQLTVQAALTGDKRMVRQAAMVDPNTAATLSVDRIWELCDELTAAHGDLIPEALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4D2</accession>
    <name>U5W4D2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560341.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28715"/>
    <dbReference type="EnsemblBacteria" id="AGZ44004">
        <property type="protein sequence ID" value="AGZ44004"/>
        <property type="gene ID" value="AFR_28715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5821"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_11"/>
    <dbReference type="OrthoDB" id="1582456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="29263" checksum="C96B7F72E57A188D" modified="2014-01-22" version="1">MTEKKVLLVTGAGRGMGVDIAQAALAAGYAVVATGRDPLKVEKALGAAEDLLTVRLDVTDRASAEAAVQAAVDRFGRIDVLVNNAGNFIAGFFEEISPEDFRAQVETNLFGPLTVARAVLPVMRRQRSGLVVSISSVAGFLGAEFTSAYAAAKFGLEGWMESLAPEVAPFGIRTMIVEPGFFRTDLLTPESTTFAEASIADYAERTEQTITAWTGMNGLQGGDPAKLAAALVHLTSSGEPPARWVAGADAVGALEQKAKTLLEQAGAHRQLSSSLDHDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4F0</accession>
    <name>U5W4F0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363260.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22655"/>
    <dbReference type="EnsemblBacteria" id="AGZ42801">
        <property type="protein sequence ID" value="AGZ42801"/>
        <property type="gene ID" value="AFR_22655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4592"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077963_1_0_11"/>
    <dbReference type="OrthoDB" id="1909281at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21746" checksum="3336325BAFAE009C" modified="2014-01-22" version="1">MIEDLRAALTRVPGKEWLDDALACVRSDPDTLGPSFARAERKLGREPLFPDSAWTAGRAGRALLLAEVGDPERIVTLYQQGDAAERLAVLVTLPLLDAGDTFVPLLHDALRTNDTRLVAAALGPYAEHLDAATWRQGVVKCVFMGIPLSAVDRLDDRADQELAGMLAALATERAAADRRMPADALALLDRLSLANPTRKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4K0</accession>
    <name>U5W4K0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363372.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22955"/>
    <dbReference type="EnsemblBacteria" id="AGZ42861">
        <property type="protein sequence ID" value="AGZ42861"/>
        <property type="gene ID" value="AFR_22955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4652"/>
    <dbReference type="eggNOG" id="ENOG50345NX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840809_0_0_11"/>
    <dbReference type="OrthoDB" id="2057947at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="15367" checksum="FA7EA61ACA25ACFA" modified="2014-01-22" version="1">MSQFFDRFRRPAEPTDEETAEAMRDLAEVFRSGSKAEGVPFGWGPDEADRLDGLCDAFLATGPTAERRHSVVMSMGAFLGELLVRNGGGLWAYDTKENAAVVVLPNGLRAFPHNKVAKRLDLGPEQSISAFYRYALTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4U2</accession>
    <name>U5W4U2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two-component regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28830</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560364.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28830"/>
    <dbReference type="EnsemblBacteria" id="AGZ44027">
        <property type="protein sequence ID" value="AGZ44027"/>
        <property type="gene ID" value="AFR_28830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5844"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="147"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="23392" checksum="8BE1464DEE824387" modified="2014-01-22" version="1">MTTVLIADDQPLQRLGFRMLLDGTAGMVVVGEASSGAEAVRMVDQTHPDVVLMDVRMPGMDGIEATRRITAAGSRTRVLILTTFDLDEYVYAGLRAGASGFLLKDARPDELIAGIRAVAGGDSVVAPSLTRKLMEAYLQDPAPAADTDPRLSALSEREREVLQAIGRGATNTEIADRFTLSESTVKKHVGRVLAKIGARDRIQAVIFAYDNGLVQPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W525</accession>
    <name>U5W525_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560581.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29915"/>
    <dbReference type="EnsemblBacteria" id="AGZ44244">
        <property type="protein sequence ID" value="AGZ44244"/>
        <property type="gene ID" value="AFR_29915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6059"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078449_0_0_11"/>
    <dbReference type="OrthoDB" id="1991518at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="28217" checksum="43A42A2F28E23121" modified="2014-01-22" version="1">MAKRVVKSFGTGALVARAALTWIRSDPRAAQLERTNFHGRTVTLAGGPALAAGATIGAVVGSTSPAVAAAVFTAGATSGAVGFYDDIAGNRPEQKAAKGFAGHLGALREGRVTSGLVKIAGVGAAGLAGAALLAGDPGVRAHRGRRKGGAVRRATDVLLGAGVIAGTANLLNLLDLRPGRALKAGALIGTPLAVGRGAGVAAGTLGAGAALLPSDLGEDIMLGDSGANALGALLGVALAARTGPVGRAVALSVLAALTAASEKVSFTKVIQDTPWLRELDALGRRPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W532</accession>
    <name>U5W532_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription regulator ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24035"/>
    <dbReference type="EnsemblBacteria" id="AGZ43075">
        <property type="protein sequence ID" value="AGZ43075"/>
        <property type="gene ID" value="AFR_24035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24035"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077964_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="31444" checksum="9D3412CE3BB7ADCC" modified="2014-01-22" version="1">MTAPALARLATLLADTTRASFCLALLDGRAWTAGELAGHAGVAPATATEHLHKLVAAGLLTEHRQGRHRYVRLADERVAHLIEDLAAHLSPPPAPPRTLRAATVSAALARGRTCYDHLAGRLGVAVTDALDRGGLLSRTAGFTLTPAGVTWFEAFGPLGPSRRPAARGCLDWTERREHLAGAAGALLCRHFLDQGWVTRIGTGRAIRVTPPGTEALTDLLGPDPAWTSPADGAVAVRVGAGRAGLVDGGQGDDGVGALGDGLVAGVAVEVGGRVARVDHVDADAGEELGVHEGEHVDRGLAGGIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5N4</accession>
    <name>U5W5N4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560846.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31265"/>
    <dbReference type="EnsemblBacteria" id="AGZ44513">
        <property type="protein sequence ID" value="AGZ44513"/>
        <property type="gene ID" value="AFR_31265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6328"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_4_0_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019945">
        <property type="entry name" value="F420_G6P_DH-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03557">
        <property type="entry name" value="F420_G6P_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="34186" checksum="D3B1F928225ABE86" modified="2014-01-22" version="1">MRIGYFLSSEEYTPAELLEQAKGAERAGFSGLWISDHYHPWVDAQGQSAFVWSTIGALSQVCSLPITTAVTCPTVRIHPAVIAQAAATSAVLTGGKFRLGLGTGEALNEHIFGDAWPEADVRLEMLEEAVEIMRELWKGGVVSHRGKHYTVENARVYTLPEKPVEILISGFGPKATSVAARIGEGYVSTMPDGDLVRQFRSTGGGDRTCQAGFKAAFAATEEEGARIAYEKWPNAGVPGELSQVLPSPKHFEQAAQLVTQDQVKEAFVCGNDPAAHLEMIEKYAAAGFDEVYVANTGPNWQGLFDLYAEHVLPKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5T9</accession>
    <name>U5W5T9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31245"/>
    <dbReference type="EnsemblBacteria" id="AGZ44509">
        <property type="protein sequence ID" value="AGZ44509"/>
        <property type="gene ID" value="AFR_31245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6324"/>
    <dbReference type="HOGENOM" id="CLU_150717_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665722" evidence="1">
        <location>
            <begin position="26"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="10760" checksum="4E075D0F66FA8080" modified="2014-01-22" version="1">MNTAAPRIAALFTSAALGLALLAGCSSDNVSCGLDQCTVTLERSTSASASVLGVEAKFVSADANTVTLEVAGEQIQLTKGQQAVDVAGLQVTLDSVTADNVGFVVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5U5</accession>
    <name>U5W5U5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363901.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25225"/>
    <dbReference type="EnsemblBacteria" id="AGZ43311">
        <property type="protein sequence ID" value="AGZ43311"/>
        <property type="gene ID" value="AFR_25225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5112"/>
    <dbReference type="eggNOG" id="ENOG502Z7XA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053011_1_0_11"/>
    <dbReference type="OrthoDB" id="1070946at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="28230" checksum="04D6144078468B96" modified="2014-01-22" version="1">MDSESIERFTTDGFIKMEQAVPPDVTAACAELLWEQIEAMPGDRSTWTRPVYWVGNMAQAPFSAAANTAVLHDAFDALVGPGRWKPRGSLGSFPLRFPHDEEPDDAGWHIEGSYQPPGESGYWVNVHTTGRALLMLFLFTDVGEGDAPTRIRVGSHLDVPPVLAPYGERGTPMMTVGSAVAAASEHRPVTLATGRAGDVFLCHPFLVHAAQPHHGEHPRFMAQPPLDPSDDLWLGPYNLRVTDAEPPPIGATIRTALHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W616</accession>
    <name>U5W616_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Urea ABC transporter permease UrtC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_30985</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560792.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30985"/>
    <dbReference type="EnsemblBacteria" id="AGZ44457">
        <property type="protein sequence ID" value="AGZ44457"/>
        <property type="gene ID" value="AFR_30985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6272"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_0_0_11"/>
    <dbReference type="OrthoDB" id="1425262at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017778">
        <property type="entry name" value="ABC_transptr_urea_perm_UrtC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482:SF4">
        <property type="entry name" value="PTHR30482:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03408">
        <property type="entry name" value="urea_trans_UrtC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="363" mass="38762" checksum="31A50AFEE48FC644" modified="2014-01-22" version="1">MTLTWSKRAAFVAVALLLFVAPLLFTPFRLDLLAKYLCYAIVAVGIALAWGRGGMLTLGQGVFFGLGGYAMGMHLKLVEAGPGQLPDFMSWSGVEQLPLLWKPFANPVFALVMVVVLPVTVALLLGLLIFRQRVRGAYFAVLSQALAAAFVILLVGQQGLTGGTNGLTNFQLFFGLDLYDDADRRILYFVIVVVLGLSYLAVRQLTRSRYGQLLVAIRDGEDRVRFLGYDPALVKTLAFAVSAGLAGIAGALFVPVVGLLTPANLGVIASLELLIGVAIGGRYSLAGAVGGALLFGYAKTIFSESWPDGWLYVQGALFVAVMLWAPRGLAGLANQLRDAVAARRRPPPPVPVPVTLTPEEVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W670</accession>
    <name>U5W670_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31210"/>
    <dbReference type="EnsemblBacteria" id="AGZ44502">
        <property type="protein sequence ID" value="AGZ44502"/>
        <property type="gene ID" value="AFR_31210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6317"/>
    <dbReference type="eggNOG" id="ENOG5033CPE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187779_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="66" mass="6743" checksum="DF268302DC7950CC" modified="2014-01-22" version="1">MKGWPWLTGGALALVIGVLWTAQGFDLLGGSVMSGVTIWAVIGPVVAVAGLALIVVGVRARARSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6J6</accession>
    <name>U5W6J6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dienelactone hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32900"/>
    <dbReference type="EnsemblBacteria" id="AGZ44838">
        <property type="protein sequence ID" value="AGZ44838"/>
        <property type="gene ID" value="AFR_32900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6658"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042393_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="40"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="474" mass="48713" checksum="771B55DC7884C08C" modified="2014-01-22" version="1">MLVTAAVGLWLLTGHRSTRQTVADGVPLVEVHPRLAPGERRPGVVVAHGFAGSARLMAPFGDSLAERGYVVVLLDFSGHGANTRPLPDSAASTAASTAALQHDLDVATRHLRGLPDVDPSAIALVGHSMGAGAVTRYAVAHPEITATVALSLPDAAPVLPDPPARLLVLVGALEFPGFRSEAARAVEPAGPDRSMVVVPGVEHIGILYAPRTHREVAAWLDQSFDTTGDPMPSPIRRLGGAALLVVALLAGLYPLARLLFGDARGSWPSPRIADLGRGVAVAAGAAALGALAARFLPTNRLPLAIGGYLLGLTGAAGAAMLAFRHRAPAIAPTGRLRLALATPVLIGYAAVTIAVPTHLGLTHAVPVGKRWWVLLLLWAGFALLAWAAGRLTAGNAFGGLAVSALVVIALTGAAVAGLTNGFVLLVVPLLAILMLWQAAWSAVLHRFAAPAWLIALVGSLLVAWPMATALPLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Q2</accession>
    <name>U5W6Q2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_32120</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32120"/>
    <dbReference type="EnsemblBacteria" id="AGZ44682">
        <property type="protein sequence ID" value="AGZ44682"/>
        <property type="gene ID" value="AFR_32120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6503"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="15"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="37424" checksum="2D8DC7C13BBAD001" modified="2014-01-22" version="1">MTAGTGRVPVGERLPTLEDVARVAGVSRATVSRVINGIRNVDPQLHEMVWNAVGQTGYVPNRLARSLVTRRTGTVALVVSDSESHDDDPFMGRFFADPYFGRVVGGLMSVLRTAGIQLALQIVGTEEQRTRLVGDLRHGLADGVIVLSLPGHDPLPRMLTDAGVPAVLIGRPADPVPINYVDLANDTGASLAADHLVTRGCQLIGMISGPADVPASNDRITGFRRSMARHGHAWVPLVSGNFTQESGEEAMRTLLAENPRLDGVFVANDLMALGALLALRDAGRQVPADVAMVGFDDSSAAVAASPALTTVRHPLEDMAAESARLLLARVEDPTMRISSVIYEPTLIVRQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6V4</accession>
    <name>U5W6V4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561216.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33105"/>
    <dbReference type="EnsemblBacteria" id="AGZ44879">
        <property type="protein sequence ID" value="AGZ44879"/>
        <property type="gene ID" value="AFR_33105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6699"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_11"/>
    <dbReference type="OrthoDB" id="1837259at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="2">
        <location>
            <begin position="14"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="16079" checksum="C2A0A7E71D5C3A59" modified="2014-01-22" version="1">MTDWDGNYEAGPHSSVHRLADVSSDMATATRSAVQAAQTAVAVIQRLEASSTEIGKVVELIATIAKQTNLLALNATIEAARAGEAGRGFAVVASEVKDLANETATATNEIGGQVGGIRSDTQNAVSAIEEMQGLIEELDRCQQVISGIVVEQQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7A1</accession>
    <name>U5W7A1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coagulation factor 5/8 type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33145"/>
    <dbReference type="EnsemblBacteria" id="AGZ44887">
        <property type="protein sequence ID" value="AGZ44887"/>
        <property type="gene ID" value="AFR_33145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6707"/>
    <dbReference type="eggNOG" id="COG5434">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009451_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14490">
        <property type="entry name" value="CBM6-CBM35-CBM36_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033801">
        <property type="entry name" value="CBM6-CBM35-CBM36-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024535">
        <property type="entry name" value="Pectate_lyase_SF_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12708">
        <property type="entry name" value="Pectate_lyase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666159" evidence="2">
        <location>
            <begin position="20"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="3">
        <location>
            <begin position="610"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="3">
        <location>
            <begin position="763"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="746"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="905" mass="94899" checksum="AF08D10B715D7CC3" modified="2014-01-22" version="1">MAAASVTAVITWSASPASAAGLSPFDIVGRGATVPFVETEAEAAATNGTKTGTSRYYGQLSSEASGREAVTLDAVGEYVEFTLTKPANAVTFRYSLPDGNNGAGRDASLDVRVNNAVVKSVPVTSKYSWFYGGYPFNNNPGDSNPHHFYDEARTLFGTTYAAGTKVRLQVSSTAQSPTFTIDLADFENVPGPIGKPANAIDVVADYGADPTGATDSTAKIQSAVNAGSAAGRTVYIPQGNFTLYSHVIVDRVTLVGAGPWYTVLGGRHPTQRNLAAGLYGKFNNQGGPSQNVTVKDLAIIGDIRERVDDDQVNAFGGAMSNSTIDNVWMQHTKVGAWMDGPMDRFTIKNSRILDQTADGVNFHIGVTNSTVTNTFVRNTGDDGLAMWAESVPNVGNSFTRNTVVAPILANNLVSYGGRDIVMTDNVVSDTVTNGGGIHVANRYPGVNGATGVQGTWTLARNTLIRAGNSDYNWQFGVGAIWFSALNEAFQNPTINITDTDILDSSYSALMWIEGQTNGIHLSNVNIQGAGTYALQVQAPSQVSFNNVRASGIAQAKPIHNCVGPSFQITRTGTNTGWFVDPPDCGPWPAPQWGNTTTPPPTTTPPTTPPTTPPTTPPPASGNLAQGKPVTVSSTSGPYVGANAVDGNAATYWESTNNAFPQTYTVDLGSSQNVGRLVLKLPPGWESRSQTIAVSGSADGSSYSSLVGATSYAFNPQATITLPSGSRRYLRLTFTANTGWPAGQLSELEAYGDGGTTTPPTTTPPPPSGNLAAGRPVSATSQAEVYTPANVADGNANTYWESTSNAFPQSLTVDLGQTRSVSRVVLKLPPAAAWATRTQTLSVLGSADGSSYSTVKASAGYTFNPSSGNTATVSFTASSQRYLRLTFTGNTGWPAGQISEFEAYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7I2</accession>
    <name>U5W7I2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Epoxide hydrolase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33570</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561309.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33570"/>
    <dbReference type="EnsemblBacteria" id="AGZ44972">
        <property type="protein sequence ID" value="AGZ44972"/>
        <property type="gene ID" value="AFR_33570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6794"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019414_0_1_11"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EHN" evidence="4">
        <location>
            <begin position="2"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="365" mass="40847" checksum="15E479098675731C" modified="2014-01-22" version="1">MITPFELHLADDTITDLRDRLRRTRWPEPATVPGWTQGVPLDYLRDYWADGYNWRAFEARIGALPQFRTTIDGLGLHFVHARSANPDAVPLILTHGWPGSIVEFAEVVGPLTESFHVVCPSLPGYGFSDRPTGPGWTVERIARAWVVLMDRLGYPTFAAAGHDWGTSITTSMAQQDPDRLLGIHLLPPLVAPDPATFDTLTDAERSARNDLSAGESGDGYSHEMSTRPQTIGYALVDSPVALAAWILEKFEAWTDGEPISRDALIDNLMLYWLPATGASAARLYWESFAEVRDRFRVGTTDKITVPTGCSIFPRENPHPSRRWAEKRFKNIRYWNEPPRGGHFAAFEQPALFVSEVQACFRALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8K1</accession>
    <name>U5W8K1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NDP-N-acetyl-D-galactosaminuronic acid dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_36385</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561869.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36385"/>
    <dbReference type="EnsemblBacteria" id="AGZ45533">
        <property type="protein sequence ID" value="AGZ45533"/>
        <property type="gene ID" value="AFR_36385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7363"/>
    <dbReference type="eggNOG" id="COG0677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_3_2_11"/>
    <dbReference type="OrthoDB" id="295459at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028359">
        <property type="entry name" value="UDP_ManNAc/GlcNAc_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500136">
        <property type="entry name" value="UDP_ManNAc_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="4">
        <location>
            <begin position="319"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="420" mass="45036" checksum="8C345C252117B39C" modified="2014-01-22" version="1">MTYDVVILGLGYVGLPLAQQATRAGLSVLGFDVNAGVVEALGAGRSHVDDLSDADVAEMIAGGFRTTADESRIAEADVAVICVPTPLSEGDGPDLRAVTGATEAIGRNLRPGMLVVLESTTYPGTTDDVVRPLLEKLSGLVAGRDFHLAFSPERIDPGNEKFGAHNTPKVVGGYTPGCTDRVAAFYGRFVQTVVRTRGTREAETAKLLENTYRHVNIALVNEMARFCHELDIDLWDVINAASTKPFGFQAFYPGPGVGGHCIPIDPNYLSHNVRSKLGYPFRFVELAQEINATMPAYVARRAQNLLNADGQAVHGATVLLLGVTYKANIADQRESPATPLARQLTALGATLTYHDPHVTRWSVGGAEVPRTDDLEGAAAAADLVILVQHHREYDADHLAGIAKRFFDTRGVTSSSEAHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8R7</accession>
    <name>U5W8R7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dihydroorotase</fullName>
            <shortName evidence="5">DHOase</shortName>
            <ecNumber evidence="5">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">pyrC</name>
        <name evidence="7" type="ORF">AFR_30625</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560721.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30625"/>
    <dbReference type="EnsemblBacteria" id="AGZ44385">
        <property type="protein sequence ID" value="AGZ44385"/>
        <property type="gene ID" value="AFR_30625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6201"/>
    <dbReference type="eggNOG" id="COG0044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_0_11"/>
    <dbReference type="OrthoDB" id="1319925at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01317">
        <property type="entry name" value="DHOase_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_B">
        <property type="entry name" value="PyrC_classI_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="6">
        <location>
            <begin position="44"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="317"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="421" mass="44277" checksum="FAA315BCA1A49BEC" modified="2014-01-22" version="1">MTSYLIKGVSLLGAAPADLLIKDGVIATGSAEGAEVIDATGLVALPGLVDLHTHLREPGREDAETVETGSRAAALGGYTAVFAMANTSPVADTAGVVEQVWRLGREAGLVDVQPIGAVTVGLAGKQLAELGAMATSAANVRIFSDDGHCVADPRLMRRALEYVKAFDGIIAQHAEEPRLTEGAQMHEGEVSTRLGLTGWPAVAEEAIIARDVLLAEHVGSRLHVCHVSTAGSVEVLRQAKARGVRVTAEVTPHHLLLTDELAASYDPVYKVNPPLRTQKDILALRQALVDGVIDIVATDHAPHAVEDKECEWAYARPGMVGLETALPVVLSVLGEEWDLIAERMSRAPARIAGLTGHGTDLTAGQPANLTLVDPSARRVVDPAELASRSRNTPYAGTTLPGRIVATFLRGEPTVLDGKAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8T4</accession>
    <name>U5W8T4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoenolpyruvate guanylyltransferase</fullName>
            <shortName evidence="1">PEP guanylyltransferase</shortName>
            <ecNumber evidence="1">2.7.7.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fbiD</name>
        <name evidence="2" type="ORF">AFR_36660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Guanylyltransferase that catalyzes the activation of phosphoenolpyruvate (PEP) as enolpyruvoyl-2-diphospho-5'-guanosine, via the condensation of PEP with GTP. It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H(+) + phosphoenolpyruvate = diphosphate + enolpyruvoyl-2-diphospho-5'-guanosine</text>
            <dbReference type="Rhea" id="RHEA:30519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:143701"/>
            <dbReference type="EC" id="2.7.7.105"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CofC family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.105" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36660"/>
    <dbReference type="EnsemblBacteria" id="AGZ45588">
        <property type="protein sequence ID" value="AGZ45588"/>
        <property type="gene ID" value="AFR_36660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7416"/>
    <dbReference type="eggNOG" id="COG1920">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076569_0_0_11"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043814">
        <property type="term" value="F:phospholactate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02114">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002835">
        <property type="entry name" value="CofC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392">
        <property type="entry name" value="PTHR40392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01983">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03552">
        <property type="entry name" value="F420_cofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="22395" checksum="335866E2DFF42DA5" modified="2014-01-22" version="1">MSGADWTAVIPVKRLSAAKSRLRGAVLDARHEELALAMVRDTVTAVVLCARVSEVLVVTDDPAASAAVAALGARAVPDRPGAGLNAAMRFGADLVAGLSRRRAVLAGDLPALRPDELGEALEAAGPGRSFVADAAGTGTVLLTADAGQPLDPHFGEGSAAAHSASLARTLTGSWPGLRHDVDTAADLRTVLALGAGEHTCGLLRDLGLVADCGSGGVPAGPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Y4</accession>
    <name>U5W8Y4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative pre-16S rRNA nuclease</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_30705</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YqgF HJR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560736.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30705"/>
    <dbReference type="EnsemblBacteria" id="AGZ44401">
        <property type="protein sequence ID" value="AGZ44401"/>
        <property type="gene ID" value="AFR_30705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6217"/>
    <dbReference type="eggNOG" id="COG0816">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098240_0_1_11"/>
    <dbReference type="OrthoDB" id="2075502at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000967">
        <property type="term" value="P:rRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16964">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00651">
        <property type="entry name" value="Nuclease_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317">
        <property type="entry name" value="PTHR33317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00250">
        <property type="entry name" value="RNAse_H_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="YqgFc" evidence="3">
        <location>
            <begin position="7"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16452" checksum="FDDA61F94C3D4492" modified="2014-01-22" version="1">MNAMPRGRRLAVDVGKVRVGVALSDPDGILATPLVTLARDMEAAADAVPRDIAELVRLVGEHEAVQIVVGLPVRLNGAEGAAAIDIRAYAGRLERAVGHVPIVLADERMSTVVATRRLAERGVRGKRQRAVVDQAAAVEILQSWLDAQRRQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W992</accession>
    <name>U5W992_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integral membrane sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_37595</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562108.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37595"/>
    <dbReference type="EnsemblBacteria" id="AGZ45773">
        <property type="protein sequence ID" value="AGZ45773"/>
        <property type="gene ID" value="AFR_37595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7604"/>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_30_11"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="186"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="417" mass="43876" checksum="ACDAA01A28DC3905" modified="2014-01-22" version="1">MDADERSIVRRARLRIGVLVGLAIGALLMLGGGISYAALLHSQENLIAGELEWGATRGTTSGPPGCSWIFVSDGRTVDTGVNPPPPGFPLQSALDTVATTRATETTKIARNGTVYYVRTQTRGNGVVQAVFDARFQLADRRHLLLAFSLAAAAGLIAAVLTGVLVARRAVAPLAEALARQRRFVADASHELRTPIAQVHTRAQLLARRARTGATAADQRDLDRLVGTTRRLGEIVDELLLSARLAATPADRPPTAAVDLAELTRAAIVTEADRAGERHVSVTLDAPGDPLPVPGIESALRRVVGELLANALTHTPEGGRIGVTVRAGNGHAELIVADTGDGFDQADADRIFDRFHRGAGAGDRRFGLGLALLREVVTSHHGTIEADGHPGRGATFTVRLPTGVASNRSTTFRGGSHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9N3</accession>
    <name>U5W9N3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_31805</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31805"/>
    <dbReference type="EnsemblBacteria" id="AGZ44621">
        <property type="protein sequence ID" value="AGZ44621"/>
        <property type="gene ID" value="AFR_31805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6438"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_419027_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024655">
        <property type="entry name" value="Unchr_glyco_hydro_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11790">
        <property type="entry name" value="Glyco_hydro_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="33"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_cc" evidence="7">
        <location>
            <begin position="410"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="349"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="354"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="630" mass="66844" checksum="9CB7C66D3BA5F269" modified="2014-01-22" version="1">MLHGEVTTPMAFQPDTATVVAARAGDPAAVDRLVAGYLPLVYTIVGRALDGHADVDDVVQDTMLRVLRNLGDLREPEAFRSWLVAITVRQVRERYRVRRASPDALLPDDVRDPGADFTDLAITRLELAGQRRETAEATRWLDEDNRELLSLWWLEASGELTRDEIVSAIEVTRQHAAVRIQRMKGQLETARVVVRALAASPPCADLGLLTADWDGRPGALWRKRIARHTRDCRQCSPAWSGLVAAEKLLVGMALVPLPHSLGGGGLAALGGAHTAAHAAGSHVAHAVSGKALAAKIAVKGGVGVGRRVLTAMLATGAVAGGGAAAVVYTNDDKPAANAAVVSTAAPAPKLSLPAPTPSATSVSPSAKPSPTKKKTKAPVVPVPPTETSAKKGVGTWQFAGIKGALDDVGAGWYYNWAPDNDSMPAPSDVEFVPMIWGKANVTSATFAKAKKEGDVLLGFNEPDMGGQANMSVEDALADWPKLQDTGMRLGSPAVAYGGDTDGGWLDRFMKGAKAKNLRVDFITLHWYGSDFSDAAVGQFLGYVDAVHKKYDLPIWVTEYGLMNFTGSPKYPTGAQEAAFIKGTTAGMEKRSFVERYAWFGLPAVGDSVDFGLYKDSSTPTEAGKAYKAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9U9</accession>
    <name>U5W9U9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative membrane-bound oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_37715</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562132.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37715"/>
    <dbReference type="EnsemblBacteria" id="AGZ45797">
        <property type="protein sequence ID" value="AGZ45797"/>
        <property type="gene ID" value="AFR_37715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7627"/>
    <dbReference type="eggNOG" id="COG2041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_2_0_11"/>
    <dbReference type="OrthoDB" id="1345710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="SSF56524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665700" evidence="2">
        <location>
            <begin position="23"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Oxidored_molyb" evidence="3">
        <location>
            <begin position="226"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="499" mass="52617" checksum="3247ED0B0F1ACF76" modified="2014-01-22" version="1">MRRFIRPALIGVLSAAFGVALAELLAAATRPQAGPLVAVGGAVIDAAPTPLKEFAVRTLGTNDKPVLLGTIALVLILFTAAIGVLSVRRRWVAVAGAAVFGLAGALAAAMRPTATAYDVVPSLLGAVVAGATLLFLHHRTRRPSATGGDFDRRAFLRAAAAVAGGVVVAGGASEVVKRVRGGAVTKSREAISLPAPADPAKPLPAGVGPGFVTANKDFYRVDTALSVPRIDVNTWSLRIHGMVGKEVDLSFDDLLKRPLIERDITLNCVSNEVGGPYIGTAKWLGVPLAPLLRELGVEPGADQVVARSAEGMTIGTPVETIFDGREAMLCVGMNGEPLPLEHGFPVRMLTPGLYGYAGACKWITELELTTFDRFDAYWVERGWGARGPVKTASRIDKPKPFAKLEAGKLTVAGVAWAQGRGIRNVEVRVDDRPWAKAELLPVPSTDTWVQWRYDWAATAGAHTIAVRATDSTGAVQTEQRATPFPDGATGWHTITASVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA59</accession>
    <name>U5WA59_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">50S ribosomal protein L36</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpmJ</name>
        <name evidence="5" type="ORF">AFR_32815</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_015624451.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32815"/>
    <dbReference type="EnsemblBacteria" id="AGZ44821">
        <property type="protein sequence ID" value="AGZ44821"/>
        <property type="gene ID" value="AFR_32815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6641"/>
    <dbReference type="eggNOG" id="COG0257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135723_3_1_11"/>
    <dbReference type="OrthoDB" id="2066169at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_L36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_L36_sp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="SSF57840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="40" mass="4677" checksum="31D539CD404E448B" modified="2014-01-22" version="1">MKVRNSLRSLKSKPGSVVTRRRGRMVVINRKNPRWNGRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA92</accession>
    <name>U5WA92_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold-shock DNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_38465</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_015625344.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38465"/>
    <dbReference type="EnsemblBacteria" id="AGZ45947">
        <property type="protein sequence ID" value="AGZ45947"/>
        <property type="gene ID" value="AFR_38465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7782"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_0_3_11"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="67" mass="7318" checksum="31B5D1E79674AC45" modified="2014-01-22" version="1">MVTGVVKWFNADKGFGFITPDDGGADVFAHFSAIQTSGYRSLDENQRVEFEVTQGQKGPQAANIRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAD1</accession>
    <name>U5WAD1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TfoX_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561287.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33460"/>
    <dbReference type="EnsemblBacteria" id="AGZ44950">
        <property type="protein sequence ID" value="AGZ44950"/>
        <property type="gene ID" value="AFR_33460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6772"/>
    <dbReference type="eggNOG" id="COG3070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136016_4_1_11"/>
    <dbReference type="OrthoDB" id="2040341at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007076">
        <property type="entry name" value="TfoX_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04993">
        <property type="entry name" value="TfoX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TfoX_N" evidence="1">
        <location>
            <begin position="15"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="108" mass="11823" checksum="815D2E6913F40A35" modified="2014-01-22" version="1">MAYDEALAERVRERLGVLPGVSDKRMFGGLAFLTEGNLTVCVTGDDLMVRVGKDTYDQALTLPGVRPVDMSGRPMRGWVVVDGAELDDEVLDGWLDRASVFVRALPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAK9</accession>
    <name>U5WAK9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="7">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_39895</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562566.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39895"/>
    <dbReference type="EnsemblBacteria" id="AGZ46233">
        <property type="protein sequence ID" value="AGZ46233"/>
        <property type="gene ID" value="AFR_39895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8072"/>
    <dbReference type="eggNOG" id="COG0303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_0_11"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6 7" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="8">
        <location>
            <begin position="205"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="436" mass="44976" checksum="EFCCEF4FD3420C11" modified="2014-01-22" version="1">MTATADAEAAANELMPLAEYLGSVLRRLRPLPPLDLDLTQAHGNVLANDVLAPHPYPAFDQSAIDGYAARWEDLAAAGRIGSHPSFGAFEGGPRPVRLNVVGDLGAASWRPVRLTPGTCFSVAAGAPLPIGADVVVPVHWTDQGMAAVEIMHAPKRGSGVRRAGDELPVGRVLAPAGSYVTPSMVAVFAASGIGHVVVRPSPRVVVVATGDELVDVGRPSQPGQVVDANSHALTAAAVEAGALAYRIGICDDDPEGLRGLLEDQTLRADLIITTGGTGTGPGDMLRRVLSRRDHGRGTVEFTDVALCPGATLGFGTVGGEEVPVVCLPGEPGAALIGFEVLARPVIQLLAGSEPVFRTSVKAHLLETVSSPGGLREFRPAHVAERRGGGYTVQPLAGGPYTLSGLAEANGLLVLGERVTSAAAGSTVDVLLLDRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB42</accession>
    <name>U5WB42_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40550"/>
    <dbReference type="EnsemblBacteria" id="AGZ46364">
        <property type="protein sequence ID" value="AGZ46364"/>
        <property type="gene ID" value="AFR_40550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8207"/>
    <dbReference type="eggNOG" id="COG0615">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046916_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="329" mass="36739" checksum="98EB19875772B8C7" modified="2014-01-22" version="1">MLHAHPRIAIPPETRFMLTAYWERRDFGDLSVASNRRALARRIVDRRETRFCDLGLDAEDIVERITAGPGTLGSALGTIFQQYAARFDKPRWGDKRPAYLHNIDILLRLFPDAQFINIVRDGRDCVASLKEMSWHRKDIYATVAAWARAVDDARRAARQLGPSQWHELRYEDLVADPHGKLGELCAYLGEDYDPAMAEPSAVADVAVPSFKTWHARTHTPVTTQRVQSWQTRLTSEEIALCEAALGSRLESSGYELSGTLRPAPAQLLRYGISAAPQRLIPARRGLSRAAGLLRRTDDLAYAPTVVLPQRTAPTPLTGHAETKHLSNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBS3</accession>
    <name>U5WBS3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562969.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41920"/>
    <dbReference type="EnsemblBacteria" id="AGZ46638">
        <property type="protein sequence ID" value="AGZ46638"/>
        <property type="gene ID" value="AFR_41920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8482"/>
    <dbReference type="eggNOG" id="COG2318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097062_1_0_11"/>
    <dbReference type="OrthoDB" id="2047691at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007061">
        <property type="entry name" value="DUF664"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04978">
        <property type="entry name" value="DUF664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="190" mass="21201" checksum="7CACEAB52E77468C" modified="2014-01-22" version="1">MPGQVGAIANERDGLLAYLTQMRYVLRLTAYGLSPEQLRAAPTASELTVGGLIKHCSSTEEGWIDTVRREPREVDYNAYAKNFVLEDGETIDDVFADYDRVAARTEKTIAEIADLGQDVPVDHSMPWNPKDLDNWSVRWVLLHLIQETARHAGHADIIREAVDGGTAYPLMAAAEGWPESPWMKPWKPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC16</accession>
    <name>U5WC16_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563000.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42075"/>
    <dbReference type="EnsemblBacteria" id="AGZ46669">
        <property type="protein sequence ID" value="AGZ46669"/>
        <property type="gene ID" value="AFR_42075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8514"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055817_1_1_11"/>
    <dbReference type="OrthoDB" id="1060221at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043917">
        <property type="entry name" value="DUF5753"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19054">
        <property type="entry name" value="DUF5753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="24"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="32959" checksum="2A3DC606F70EF2D4" modified="2014-01-22" version="1">MSAGSQEESPNSGPTVLRILLGAQLRRLREGKGISREDAGYEIRASGSKISRMELGRVSFKERDVADLLSMYGVKDAAERDALLGLARQANNPGWWHHYGDILPPWFQSYLGLEAAASLIRTYEIQFVPGLLQTPEYARAVILRGHAGANADEIDRRVELRRQRQQILDRIEPPQLWAVIDEAVLRRPIGGPEVMRAQIEALIEAAKKPNVRLQIIPFNAGGHAAAGGPFAILRFPEPELPDVVYVEQLTSAIYLEKRDDVDHYAIAMERVCIDAEPPNHTQEILGKLLHEVGRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCN5</accession>
    <name>U5WCN5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-dependent synthetase and ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562100.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37555"/>
    <dbReference type="EnsemblBacteria" id="AGZ45765">
        <property type="protein sequence ID" value="AGZ45765"/>
        <property type="gene ID" value="AFR_37555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7596"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_11"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="19"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="122"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="381"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="463" mass="48545" checksum="D5A43ED7E47CBAB3" modified="2014-01-22" version="1">MPTDVWPQPILDLLAAGGDRPVFEDGPRVVGAGELLVLVRRAAAGLRAAGIGPGAGVAVLAGVSAEAFAATIAAFAVGARVSGIRPGLPPEQLADLLSRDHAVLIGPDELDGLLAAPDEPLVAAGRPDDIARIIYTSGSTGRPKGCVQTYAAMSAAWAPYPDRWPPAIRELAGRLDRYLVFGSLSSQVMLEYGILTLAAGGTLVAAHAPAFPDSLVRHRATASVITVGKLHRLVRDQQEHPVDLSNLGALMVSGSPLEPGRLREALEVLGPVVFHGYGQTETGMIAMSTPDSASSEAALSSVGRPPAVVDVSIRDGELYVRTPSQASGYWHEPEESAEVFVDGWVRTRDLASLGDDGLLRLLGRVRDVIIVNAELLYAGPIERALATDPDVAEAYVVGRPDEDTGEAIHAYVVPVNGRTPDTGTLRKLVGERLGPNAVPRTVTLIDRVPVAPSGKPDKTQLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCR8</accession>
    <name>U5WCR8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit beta</fullName>
            <ecNumber evidence="11">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP synthase F1 sector subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">F-ATPase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">atpD</name>
        <name evidence="13" type="ORF">AFR_37335</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562056.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37335"/>
    <dbReference type="EnsemblBacteria" id="AGZ45721">
        <property type="protein sequence ID" value="AGZ45721"/>
        <property type="gene ID" value="AFR_37335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7553"/>
    <dbReference type="eggNOG" id="COG0055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_0_2_11"/>
    <dbReference type="OrthoDB" id="430176at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA" evidence="12">
        <location>
            <begin position="158"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="166"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ45721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="480" mass="52223" checksum="1D5FDB57240AE330" modified="2014-01-22" version="1">MTAVAEPTKTQAGVGRVVRVIGPVVDAEFPRDAMPEIYNALHVDVTLSGGTKTLTLEIAQHLGDNVVRAISMQPTDGLIRGAEVTDTGAPISVPVGDVTKGHVFNALGEVLNVDPSTLNITERWPIHRKPPKFADLEPKTEMLETGIKVLDLLAPYVRGGKIGLFGGAGVGKTVLIQEMIIRVARNFGGTSVFAGVGERTREGNDLILEMDEGGVLDKTALVFGQMDEPPGTRLRVALSALTMAEYFRDVQNQEVLLFIDNIFRFTQAGSEVSTLLGRMPSAVGYQPTLADEMGELQERITSVKGKAITSLQAIYVPADDYTDPAPATTFAHLDATTNLERSISDKGIYPAVDPLASSSRILAPEFVGAEHYAVAREVQRILQKYKDLQDIIAILGMDELSEEDKVTVQRARRIERFLSQNTYAAEQFTGVPGSTVPLKETIEAFKKISEGEYDNYPEQAFFMCGGLEDLEKNAHELMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCX9</accession>
    <name>U5WCX9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_37685</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562126.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37685"/>
    <dbReference type="EnsemblBacteria" id="AGZ45791">
        <property type="protein sequence ID" value="AGZ45791"/>
        <property type="gene ID" value="AFR_37685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7621"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_3_4_11"/>
    <dbReference type="OrthoDB" id="1864829at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="12"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12446" checksum="72B29CD21B8B4A10" modified="2014-01-22" version="1">MYLPITTRQLADGTVEIAPSGEIDLDNAHVMRDAVNDVLTSSTPGKIDLDLQRVMLIDSIGIGILVACFHTAAASGVKLVVSHPSPTVYRQLWISGLVGLLGCAEPPSPRTSVGLRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD70</accession>
    <name>U5WD70_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA polymerase factor sigma-70</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_38580</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562304.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38580"/>
    <dbReference type="EnsemblBacteria" id="AGZ45970">
        <property type="protein sequence ID" value="AGZ45970"/>
        <property type="gene ID" value="AFR_38580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7806"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043648_0_0_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014305">
        <property type="entry name" value="RNA_pol_sigma-G_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02960">
        <property type="entry name" value="SigX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="111"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="30989" checksum="6ACCAD08CD185931" modified="2014-01-22" version="1">MDLAALRPELLAHCYRMLGSIHDAEDAVQETLVRAWRGYDGFEGRASPRRWAYAIATRVCLTALDQRTRRALPSGLGTDEVAWLEPLPDALLHTGTPDPAGLALARSGIRLAFVAALQHLSPRQRSALVLGDVLGWPVAEVAEALGTTPASVNSALQRARAQLRRLDLDEDQIAEPAPGVLDRYAAAFEAADLPTLAGLLSADVTLEMPPRPDWYAGRDATLAFLRTNVLRPGAWQLIPARANNQPAFIVNEADGTPHGLQVLDIHDGRLTRLFAFLDPALARRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE98</accession>
    <name>U5WE98_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Flagellar motor switch protein FliN</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fliN</name>
        <name evidence="8" type="ORF">AFR_40505</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FliN/MopA/SpaO family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562687.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40505"/>
    <dbReference type="EnsemblBacteria" id="AGZ46355">
        <property type="protein sequence ID" value="AGZ46355"/>
        <property type="gene ID" value="AFR_40505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8198"/>
    <dbReference type="eggNOG" id="COG1886">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097058_0_0_11"/>
    <dbReference type="OrthoDB" id="1875967at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012826">
        <property type="entry name" value="FliN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001172">
        <property type="entry name" value="FliN_T3SS_HrcQb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00956">
        <property type="entry name" value="FLGMOTORFLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02480">
        <property type="entry name" value="fliN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliMN_C" evidence="7">
        <location>
            <begin position="172"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="25079" checksum="A3FAE430992BB795" modified="2014-01-22" version="1">MTAPTITDPQLAQVRTAAEAALAVLPTSRELLVGAPVAATADMLTTGQAITAKFSGAATGEVVVVVGQDLADALKESPLGELDLTAAVRPALEAAARAFGPVVLDPGQLMEPGVALSAIAAKDGAVAVPLLDGGDIRAVLAMALSPWPSDGAPAGVARRGDSAGPMRGGLDMLHDVEMEVSAELGRTRMSVRELLSLTPGAIVELDRAAGSPADLLVNGRLIARGEVVVIDENFGIRITEIISPGSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEB0</accession>
    <name>U5WEB0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein-glutamate methylesterase/protein-glutamine glutaminase</fullName>
            <ecNumber evidence="3">3.1.1.61</ecNumber>
            <ecNumber evidence="3">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheB</name>
        <name evidence="9" type="ORF">AFR_40580</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>[protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L-glutamyl-[protein] + methanol</text>
            <dbReference type="Rhea" id="RHEA:23236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="3.1.1.61"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CheB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.61" evidence="3"/>
    <dbReference type="EC" id="3.5.1.44" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562702.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40580"/>
    <dbReference type="EnsemblBacteria" id="AGZ46370">
        <property type="protein sequence ID" value="AGZ46370"/>
        <property type="gene ID" value="AFR_40580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8213"/>
    <dbReference type="eggNOG" id="COG2201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_51_0_11"/>
    <dbReference type="OrthoDB" id="1655418at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018277">
        <property type="term" value="P:protein deamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006482">
        <property type="term" value="P:protein demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16432">
        <property type="entry name" value="CheB_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00099">
        <property type="entry name" value="CheB_chemtxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008248">
        <property type="entry name" value="CheB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035909">
        <property type="entry name" value="CheB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000673">
        <property type="entry name" value="Sig_transdc_resp-reg_Me-estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01339">
        <property type="entry name" value="CheB_methylest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000876">
        <property type="entry name" value="RR_chemtxs_CheB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52738">
        <property type="entry name" value="SSF52738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50122">
        <property type="entry name" value="CHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="3"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="CheB-type methylesterase" evidence="8">
        <location>
            <begin position="193"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="131"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3 5">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="40817" checksum="CBFF51318546F550" modified="2014-01-22" version="1">MIKVLVVDDSVVVRRLIVDSLTGAPDIEVVGTAANGLLAQAKIDQLKPDAVTMDIEMPQMNGIEAVRELRKRHKHLPVIMFSTLSAAGASATLEALSAGATDYVTKPSNVGSIQESIAAVREQLVPKIHALAGRRRPAGPPARPGPPPGRPGLAPPAAARPGAPVPGRPGLAAARPGAPVAGGPARPVRRGGPQGRVDILAIGSSTGGPDALTKVLQGLPPDLPVPIVVTQHMPPVFTRMFAERLDRSTPLRVVEAGDGMELAPGTCYIAPGDKHLVLQRRSTATLTQLSGAPPENSCRPAVDVMFRSVASLFGAAAFATVLTGMGYDGRGGAKVLREAGAEVLAQDEASSVVWGMPGAVVGAGLADEVLPLDRIAAALVQRVQVGRGVARPAAVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEM0</accession>
    <name>U5WEM0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41180"/>
    <dbReference type="EnsemblBacteria" id="AGZ46490">
        <property type="protein sequence ID" value="AGZ46490"/>
        <property type="gene ID" value="AFR_41180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8335"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="2">
        <location>
            <begin position="304"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="40821" checksum="E7FE13FA42F03509" modified="2014-01-22" version="1">MTVSGTTPGRTAAARVGSVLIRPVPTLRWALDVLLAEAVGVLCWYAASESPLPPAGGVHEPAWVTVLVGVTLGAPLAVRRIWPVVVAVVVLVSAGVWLSLGVVPDYASTGPLAAAGITVYTVAVSVSGRRSYVVLVGSIATATAGMLIAGNSKAGPGRAEVAFVVVALGACWLVGWTLRERRASVAQATSQATAQAVIDERLRIAREIHDIVGHSLTVIAVKASIGSHVAEQRPEEASAALRVIASTSRGALTELRRAVGALRTEADFAPPPTLEELSRLAERAAEAGVQVDLTIQNGGTVPDGIALAAVRIVQESLTNVVKHVGPTTCRIKIRGGPGELQIEVANDGPPARSQTVDGAGLTGMRERVALHHGSLTAGPRAEGGFAVSATLPYEQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFN1</accession>
    <name>U5WFN1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative metal ion transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_42385</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42385"/>
    <dbReference type="EnsemblBacteria" id="AGZ46731">
        <property type="protein sequence ID" value="AGZ46731"/>
        <property type="gene ID" value="AFR_42385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8578"/>
    <dbReference type="eggNOG" id="COG0598">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007127_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="SSF143865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="SSF144083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="39786" checksum="945150F52223A7EC" modified="2014-01-22" version="1">MNRFLGTTDGVPAEPGREFHSTAVVDCGVYVNGKREPGEFTPDEALREACSRENTFVWLGLHEPTQAEMSAIARTYDLHELAVEDAVKAEQRPKLEQFGSVHFLVLRTARYVPHRELTENSQVVETGQMMIFVGERFVITVRHGDASRLAPVRADLESRGVLLEQGPWAVAYAVTDKVVDAYVEVADQVEADLDIIEEGAFSRGTGAPVQQIYQMKREIVEFRRAVVPLQRPLATITSPQSRLVPKEIRRYFRDVQDHLTRTVEQVSSYDDLLNSILQARLAQVTVDQNNDMRKIAAWAGIATVWTAIAGVEGMNFVHMPEQRWPYGYPVVFLLMLGISVLLYRAFRRNGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNG9</accession>
    <name>U5VNG9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_01120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357456.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01120"/>
    <dbReference type="EnsemblBacteria" id="AGZ38513">
        <property type="protein sequence ID" value="AGZ38513"/>
        <property type="gene ID" value="AFR_01120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.223"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="24384" checksum="05CBC071B0B4E82E" modified="2014-01-22" version="1">MSARETVLELRDVVKEYPTDPPTRVLHGVDLTVRAGELLAIVGPSGSGKSTLMNIVGTLDRPTSGQVKVAGHDVSDLSDQDLSALRGNAIGFVFQQFFLLSGMSSLDNVADGLLYHGVARQARRDRAAAALDRVGLGHRLHHNPEKLSGGERQRVAIARALVGDPAVVLADEPTGNLDSASSASIMGLLHELHAAGSTIVVITHDREIAQSLPRQVAVRDGRIEHGAFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNX0</accession>
    <name>U5VNX0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01000"/>
    <dbReference type="EnsemblBacteria" id="AGZ38489">
        <property type="protein sequence ID" value="AGZ38489"/>
        <property type="gene ID" value="AFR_01000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.200"/>
    <dbReference type="eggNOG" id="COG0510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077925_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="31275" checksum="31551D9756220543" modified="2014-01-22" version="1">MIQDLLGSAIVAEHPARAGFTPSAASVVVGSKGNRLFVKAAPVGEGLGEAVEAGVALAQVADELGPLLVGSATVKEWRVAAYEVVEGEPVKWWEAADHPRLLEVLQRMREVMDPCPVAGTSSYAEAFLPMLGTWQALNNNAGPASIGRPLPVDLPVPLLAEMESRWLDVLGGGSALHHGDLRRDNVIRERTGRLRIVDWTHLWTAPGWMDLIRLAPDVAACGHDPERLLQRSCWRDAPADEVNVALAGLAGRGWRDGHLPDIPGLPGLRRMQREQCLHTLRWLEARLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP87</accession>
    <name>U5VP87_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357542.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01550"/>
    <dbReference type="EnsemblBacteria" id="AGZ38599">
        <property type="protein sequence ID" value="AGZ38599"/>
        <property type="gene ID" value="AFR_01550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.311"/>
    <dbReference type="eggNOG" id="ENOG5031HG1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104650_0_0_11"/>
    <dbReference type="OrthoDB" id="1837726at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="22568" checksum="99C62C29F5B2DD74" modified="2014-01-22" version="1">MDHEDDELPPTDPAESLRLIESQRALTERRLTPDPRLLLWPWGVAWLIGFGVFFLRFGPDGRIFVDMPSFVPLTVLLGLITLAGIVTGVVGARAGRQVSGPSSRQGAMYGITWSVAFIGMAVLFSKVSNLLPEPEANLLWAGAMVALTGALHMAGGAVWNDRSLFILGASISFVNVVGVIIGPGWHALIVSVLGGGGMLLAGLLSWLRLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPA4</accession>
    <name>U5VPA4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative NADH dehydrogenase I chain M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_01715</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Membrane</location>
            <topology evidence="1 3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357575.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01715"/>
    <dbReference type="EnsemblBacteria" id="AGZ38632">
        <property type="protein sequence ID" value="AGZ38632"/>
        <property type="gene ID" value="AFR_01715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.345"/>
    <dbReference type="eggNOG" id="COG1008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_4_4_11"/>
    <dbReference type="OrthoDB" id="535707at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010227">
        <property type="entry name" value="NADH_Q_OxRdtase_chainM/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507">
        <property type="entry name" value="PTHR43507"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01972">
        <property type="entry name" value="NDH_I_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="461"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="5">
        <location>
            <begin position="134"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="512" mass="55109" checksum="7687B503EE2A82E1" modified="2014-01-22" version="1">MTDFPFLSILTLFPLVGAAVVAFIPKTRAQLAKTVALVWSLLVLALSVVMWIVFEAGGDRFQFRESYQWIPTWDARFTFAADGIALVMLMLIAVLVPAVILASWHDVDESKRSVPTYFALLLALECTMIGVFAAADVFLFYVFFEVMLVPMYFIIGSYGGARRQYAAVKFFLYSLVGGLFMLAAVIGLWVVGGHTFDWQKLSDVSAAFDTNTARWLFLGFFIAFAVKAPFFPFHTWLPDAGVAAPAPAAALLVGVLDKVGTFGILRYCLPLFPEASRWFAPAALILGVIGIIYAALLAVGQHDLKRLVSYTSIAHFGFIGIGIFAFTTQAGTGSVLYMVNHGLATGLLFLVVGMFVARRGSALISDFGGAGKLVPVLAGVLFFAGLASLALPGTAPFVSEFLVLIGTFTVHKVYAVIATVGIILAAAYVLWMVQRTTQGTLNPALTEVPAMHKDITLREKIVVAPLVLLLLILGFYPKPVTDVINPAVEATMQDIGTTDPAPTAVANGRVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPE9</accession>
    <name>U5VPE9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01990"/>
    <dbReference type="EnsemblBacteria" id="AGZ38687">
        <property type="protein sequence ID" value="AGZ38687"/>
        <property type="gene ID" value="AFR_01990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.403"/>
    <dbReference type="eggNOG" id="COG3226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_21_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041583">
        <property type="entry name" value="TetR_C_31"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17940">
        <property type="entry name" value="TetR_C_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20654" checksum="B3E392A912D4591B" modified="2014-01-22" version="1">MTAPARSPRRYDPERKTRIVDAAVDVIAEHGVAGTTHRRIAAAADVPLGSLTYHFDGLDDLLAQAFRRHAERMSRSYEAAFSQVETREQLIDAITDLIHGGAGADPHDWAVAYELYLAALRDPALRSVTESWMRTSRTVLERFMDATTARGVDALVEGLVMHKTLSTTRGATRAETRQIVARQLGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPT6</accession>
    <name>U5VPT6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357765.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02665"/>
    <dbReference type="EnsemblBacteria" id="AGZ38822">
        <property type="protein sequence ID" value="AGZ38822"/>
        <property type="gene ID" value="AFR_02665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.536"/>
    <dbReference type="eggNOG" id="COG3980">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023406_1_0_11"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="349" mass="36746" checksum="303AA4DB87993FBA" modified="2014-01-22" version="1">MTITIGIRCDAGPRTGVGHLLRCVALAEELTARGLAVHFLSDLGGLEWATQQLTSRGLPWHPAPYDTVGMVGAADRLGLDAVVIDSYEVPAAHSRALRASGRAVVAIVDGDLRDQDADIFVDQNLDAERRHPPLPPGAVRLAGLEYALLRHAVRDLRPATAPSGGSARTPKVVAFFGGTDAYRAAPVVGRLLVRTGMDCTATIVAADDSLRRELLALEPEPGQRFDIIAPTDDLPKLLADADLAISASGTSTWELLCLGLPAALVWVVDNQIIGYGRTTARGLAAGLGHLPDLTSSPFESGAAVDLLRTLLVSPSRRAALAARAWSAVDGRGTARVADALLDRLRPAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPU7</accession>
    <name>U5VPU7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative acyl-CoA reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357775.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02715"/>
    <dbReference type="EnsemblBacteria" id="AGZ38832">
        <property type="protein sequence ID" value="AGZ38832"/>
        <property type="gene ID" value="AFR_02715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.546"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_668632_0_0_11"/>
    <dbReference type="OrthoDB" id="1776958at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008218">
        <property type="term" value="P:bioluminescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008670">
        <property type="entry name" value="CoA_reduct_LuxC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05893">
        <property type="entry name" value="LuxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="394" mass="42435" checksum="9FFCB8324C80954A" modified="2014-01-22" version="1">MRFRFGDPGDLTAPSQDRLTVGDPRVVDFLSRFARKLLAPAVARRHPELGSLGYFLRPAELTRAAARLERPDAFVFPRGNVFHVPPANVDTIFVYSWALSALAGNHNVVRISARSAGAAETVLETLNATLEDADPVIGRTQRMVTYGRDDTITAALSAWCDLRVIWGGDSAVTTIRKHPLRPSARDLTFPDRTSWAALSVPGWRAADPAAKRAAATGFANDAYWFDQAACSSPRTVFLVGGDPAGVADEFLELLSEVVARRGWAVDAAMAVEKRVNAYGLAASGAVTAMAFPENAVTSLALAEVADAPRRWIGAGAFPFASVGKLEDLVPAMNRQDQTFSHFGFAPAELKAFALALGGRGVDRIVPFGSALTFSAIWDGFDLPREFTRLTTLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPV6</accession>
    <name>U5VPV6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxygen sensor histidine kinase nreB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_02765</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02765"/>
    <dbReference type="EnsemblBacteria" id="AGZ38842">
        <property type="protein sequence ID" value="AGZ38842"/>
        <property type="gene ID" value="AFR_02765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.555"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025828">
        <property type="entry name" value="Put_sensor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13796">
        <property type="entry name" value="Sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Sensor" evidence="3">
        <location>
            <begin position="30"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="251"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="437" mass="46554" checksum="1A923D659EE4E5E8" modified="2014-01-22" version="1">MGMDTTIAPGELSSSVAGRLFRQLGLDTQYILLGFPLGLITLVLSMAGFWVGIGTMIIWLGLPILVGTFMMARGFATVERARLAPVLGRRMPHPHYKRAAEGSTLVKRVVTPVTDGQSWLDLLHGIFRFIPSTIAFVFAIVWWVGALGGLTFPLWDWSIPRGPDEQDLPMLLGLGDAWSTRVIFHMAIGFFFAGTLYPVIRGMALLEALFARGLLSGVNELREQVAEAHAARDVAQAQKSAAVSAEATALRRLERDIHDGPQQRLVRLAMDLGRAEQQFSTDPEAARATVAEALSQTRETLDELRALSRGIAPPILVDRGLQAALTALAGRCTIPVDLDAPPLARLDPAVETTAYFVVAEALTNVAKHSYASEVQVSVQRASAGLLITVADDGVGGASLAKGHGLSGLSDRVQAAGGVMNVDSPAGLGTTLTVALPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ84</accession>
    <name>U5VQ84_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357920.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03440"/>
    <dbReference type="EnsemblBacteria" id="AGZ38977">
        <property type="protein sequence ID" value="AGZ38977"/>
        <property type="gene ID" value="AFR_03440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.692"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029929_1_0_11"/>
    <dbReference type="OrthoDB" id="1755236at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665136" evidence="2">
        <location>
            <begin position="22"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="28190" checksum="0297A0096321D13D" modified="2014-01-22" version="1">MSSLVIAVLLCIVSAAGYAAAAVLQERVAQRPLGVLVRLPVWWVAIALNGVGAALHVAALRYGPLSLVQPFGVLTLVLAVPLAATLARRAVSRVEGRGISLTVAGLVGILLLAGTTNSIAVLSTAQLVGLLLVTATVLTALGGWGAVPGASGLWTAAAAGVSFGVSSALTQTVAVHVTDEGFGALLDPTVAVAALAIAVLSTAGTLFTQRSYRDGLGAPLAVSTLANPVAAATIGLVLLGEQITGGTVGALIALASAGAAAAGVSLLTRAQLKLKVPVSVAPEVCPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ91</accession>
    <name>U5VQ91_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative response regulator receiver domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358033.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03995"/>
    <dbReference type="EnsemblBacteria" id="AGZ39088">
        <property type="protein sequence ID" value="AGZ39088"/>
        <property type="gene ID" value="AFR_03995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.807"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_11"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="13"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="17215" checksum="701B0B78781B5521" modified="2014-01-22" version="1">MTGPTRQDGTPIEVLLVEDDPGDVLMTQEAFEEHKVRNRLTVVSDGAEALSYLRREPPYEKAVRPDLILLDLNLPRRDGREVLEEVKKDDQLCRIPVVVLTTSAADEDILRSYQLHANAYVTKPVDFDRFISVIRQIDEFFVSVVKLPPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQA2</accession>
    <name>U5VQA2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03500"/>
    <dbReference type="EnsemblBacteria" id="AGZ38989">
        <property type="protein sequence ID" value="AGZ38989"/>
        <property type="gene ID" value="AFR_03500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.703"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="16403" checksum="E67F719B3E8C6CCD" modified="2014-01-22" version="1">MSATADVSAAWQAYQRMRLQLNGRISRELFRSTGLSEADFDILFFLEGSPDGSVRSIALRCGLEWEKSRLSHQLRRMEGRGLITKGADASVQLTDKGREQIAAAQACHSAAVSRYFGDVLTDEQLTALTAISDQVVSRLTQDRHHSPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQP3</accession>
    <name>U5VQP3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_04300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358094.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04300"/>
    <dbReference type="EnsemblBacteria" id="AGZ39149">
        <property type="protein sequence ID" value="AGZ39149"/>
        <property type="gene ID" value="AFR_04300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.868"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3979">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058767_0_0_11"/>
    <dbReference type="OrthoDB" id="1622507at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="GH16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665591" evidence="2">
        <location>
            <begin position="36"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="4">
        <location>
            <begin position="23"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="3">
        <location>
            <begin position="327"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="455" mass="48091" checksum="AC3DA7EFC37AF8E0" modified="2014-01-22" version="1">MGTKRRIAVSATVAATAAALAAVGIAAAPSSSAAAACGVLFDDFNYSSRTDAALSQRGWSIRGNAGGPGVPGASWLADNVAFPTVDGQKVAQLTARTNGTAAGTSHAEFSQSNRRFFEGTYLARIKFADAPVSGPDGDHVNQTFYTISPLAAPMDPTYSEMDFSEYLPNGGWGEAGPINYQTTWYTYVADPWYADNQHSQQARSINGWHDVMATVSAGHVKYFIDGVQVGDHSGKVYPRQNMSIDFNQWFIDLAGHSSGTSVYQQSVDYLYHAKKQVLTPAQASATIAAYRSAGTTHTDNVVSTNDCDGGGTTPPPATTPPTTTPPATGGTRLQSNFSGRCIDIPGGVPNSGSLLQMYDCNTSAAQTWKFEGDGTLRAMGKCMDPAGGALANGTRIQLTDCNTNPVQRFTLTAERTLKNVSSGRCVDIKDWNGSNGAGLQLWDCAATSNQIWGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRE0</accession>
    <name>U5VRE0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9 10">Chromosomal replication initiator protein DnaA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dnaA</name>
        <name evidence="15" type="ORF">AFR_00005</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 11">Belongs to the DnaA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357234.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00005"/>
    <dbReference type="EnsemblBacteria" id="AGZ38290">
        <property type="protein sequence ID" value="AGZ38290"/>
        <property type="gene ID" value="AFR_00005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1"/>
    <dbReference type="eggNOG" id="COG0593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026910_2_0_11"/>
    <dbReference type="OrthoDB" id="219876at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06571">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1750.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00377">
        <property type="entry name" value="DnaA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001957">
        <property type="entry name" value="Chromosome_initiator_DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020591">
        <property type="entry name" value="Chromosome_initiator_DnaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018312">
        <property type="entry name" value="Chromosome_initiator_DnaA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013159">
        <property type="entry name" value="DnaA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038454">
        <property type="entry name" value="DnaA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30050:SF2">
        <property type="entry name" value="PTHR30050:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08299">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00051">
        <property type="entry name" value="DNAA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00760">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00362">
        <property type="entry name" value="DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01008">
        <property type="entry name" value="DNAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="13">
        <location>
            <begin position="320"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_DnaA_C" evidence="14">
        <location>
            <begin position="532"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="80"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="174"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="110"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="328"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ38290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="626" mass="70191" checksum="FD831411980ED4A9" modified="2014-01-22" version="1">MADTVDLGGVWTAATDELADEIASAQQRAYLRLTRLRAIVEDTALLSVPDTYTRDVIESRLRLAITEALSRRLGRPIQVAVTVRPPEDGSGRPGTVYGTPPPEPPTQPPAHYADQQQHQQPAYQDHYQQGPSFGHPQTFDDQYENEGPTGSAQAPFARPVPVARDGQDALFAAPMPQQQPDSKSSAPEQKEVATRTDAEPQRREAPRQMSHFADPSNDPGGHRMAGDQSQLRDNQRRPDDRPPMHGQDRRDDTLQMRHGGDNGPGRAPMDMRGQSGDRRPGGNDGNRLNPKYMFETFVIGSSNRFAHAASVAVAESPAKAYNPLFIYGSSGLGKTHLLHAIGHYATTLGHARSVRYVSTEEFTNDFINSLRDDKTQAFQRRYRDVDILLIDDIQFLENRERTQEEFFHTFNTLHNANKQIVISSDRSPRQLATLEDRMRTRFEWGLLADIQPPDLETRIAILQKKAAQERMYAPDDVLEFIASRVSNSIRELEGALIRVTAFASLTRSSVQLSLAEEVLRDFMPDGAGPEITADQIMVSTADYFGVSLEDLRGHSRSRVLVNARQVAMYLCRELTELSLPRIGQAFGGRDHTTVMHADRKIRQHMAERRSLYNQIAELTNRIKQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRJ7</accession>
    <name>U5VRJ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357299.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00330"/>
    <dbReference type="EnsemblBacteria" id="AGZ38355">
        <property type="protein sequence ID" value="AGZ38355"/>
        <property type="gene ID" value="AFR_00330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.68"/>
    <dbReference type="eggNOG" id="ENOG50331SS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080168_4_1_11"/>
    <dbReference type="OrthoDB" id="1857091at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043231">
        <property type="term" value="C:intracellular membrane-bounded organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="24039" checksum="F028A5B8C3757061" modified="2014-01-22" version="1">MNLAEEFALLAYGDDGAPDTDTMRLDNGLGGALLLELALTGRVDVEDKKVIVRDGTPTGDPLVDQALERIVADDKQRKPGQWVTKFAKDARKLTLDRLVSQEVLVREQDKVLLVFPRTRYPAPHGGRPIPETEARQRLTAAVTRTEAVEPRTAALCALVAATDLDRKVFRDLDRNLVKTRLKEISQGAWAADAVKKAIEEIQLAIIMTVVAATSATATTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRX5</accession>
    <name>U5VRX5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_01030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357438.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01030"/>
    <dbReference type="EnsemblBacteria" id="AGZ38495">
        <property type="protein sequence ID" value="AGZ38495"/>
        <property type="gene ID" value="AFR_01030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.205"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_44_1_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039538">
        <property type="entry name" value="BetI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13977">
        <property type="entry name" value="TetR_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="21340" checksum="74D0F7C4D83E5721" modified="2014-01-22" version="1">MAEGYLKGRIRREDIITTAVSVYAEAGYHGSSLREIARRSGITHAGLLYYFPTKEALLAAVLERRDSEDTEREHLAVPPGLDALRHLIALAAHNVQHPGIVDVYARLAAEAVAAEHPAHDYFERHYKMARDYAVASFQVLAERGELRPGVDPAYAGLNLVALMDGLQVQWLSGGGAVDLVGALRSYLESLLAVPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS12</accession>
    <name>U5VS12_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ricin B-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357468.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01180"/>
    <dbReference type="EnsemblBacteria" id="AGZ38525">
        <property type="protein sequence ID" value="AGZ38525"/>
        <property type="gene ID" value="AFR_01180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.235"/>
    <dbReference type="eggNOG" id="COG3828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_681117_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029010">
        <property type="entry name" value="ThuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06283">
        <property type="entry name" value="ThuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665620" description="Ricin B-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="32"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="280"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="421" mass="45855" checksum="51998492C0D3E7DF" modified="2014-01-22" version="1">MARNLLRRVLVAALGILTAGTIGIAVRPADAAAAAPFKVLAFYNGTWDAAHIDFDKEARDWFPRAGAQYGFTWEATTDWSRLNTGNLAQYKVIMFLDDAPPSGQRAAFQQYMQNGGAWMGFHVSAFTTDANSWSWYYNTFLGSGNFATNTWGPTAETLKIENRNHPSTAGLAATIPSAVSEWYSWSRDLRQNPDIDILASVDTSSFPVGSDPNQQWRSGFYPLIWTNKNYKMLYTNFGHNAMNYETNTRTSSTFASAQQNQWLINGLLWLGGATSTPPNTPSWYALSSKNSGKCVDARAAGTTSGTAIQQYACNGSTAQQYQLAPTSDGYVRINNRGNSAQALDVTNVSTADNAPIQTWAYSGGTNQQWQPVAEADGSYHFVNRNSGKCLDVPAASTADGVQLVQYACNGTAAQSFRLTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS35</accession>
    <name>U5VS35_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Group 1 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_00960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357424.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00960"/>
    <dbReference type="EnsemblBacteria" id="AGZ38481">
        <property type="protein sequence ID" value="AGZ38481"/>
        <property type="gene ID" value="AFR_00960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.192"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_10_1_11"/>
    <dbReference type="OrthoDB" id="948440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="15"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="193"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="376" mass="39685" checksum="81CD7514C65AC3C5" modified="2014-01-22" version="1">MRIVRLANFVTARSGGLRTALRELGAGYLAAGHEPVLIVPGARYSDVDTEQGRIITLPGPEVPRMGGYRVLLDRRRVAETLRQVRPDHVEVSDRTTLRWIGRWARQHGVPSMMVSHESLDGLLRLFGGGRWTADRLNARTAADYDRVVCTTGWAAAEFERLGAGVARVPLGVDLDLFSPARHDPALRRRWAAPGDTLLVHCGRLSAEKQPERSLATLAGLRASGVPAVLVVAGGGPLRPALQASAAEQGLPVRFVGHLRDRGELAALLATADVALAPGPIETFGLAALEALASGTPVVASAASALPEVIGTAGIAATTDASFAAAVRTLLARPDRRAAARARAETFPWSASVAGFLSLITADGAVMLGSTGRRRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSC4</accession>
    <name>U5VSC4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07115"/>
    <dbReference type="EnsemblBacteria" id="AGZ39712">
        <property type="protein sequence ID" value="AGZ39712"/>
        <property type="gene ID" value="AFR_07115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1452"/>
    <dbReference type="eggNOG" id="ENOG5032DI7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165566_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="66" mass="7045" checksum="AFB5294806813B60" modified="2014-01-22" version="1">MSDDLQLLDAYLDDAFLSTERLSSGDLQRGAIAADLPATAMTRIDALPEGEYSQDEASEALRVLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSC6</accession>
    <name>U5VSC6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07375"/>
    <dbReference type="EnsemblBacteria" id="AGZ39764">
        <property type="protein sequence ID" value="AGZ39764"/>
        <property type="gene ID" value="AFR_07375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1504"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108054_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12799" checksum="B4F4916097B0E71C" modified="2014-01-22" version="1">MTLSGIVLDSPDARELAAFYQRLLGWSVVQDEPEWVKLGAPGGGPGLSFQTESAYVRPVWPAGPDDPQMSVHLDIHVEDLDQAGAHAEKAGATKADFQPQDDVRVYLDPAGHPFCLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSN3</accession>
    <name>U5VSN3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4129 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359529.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08545"/>
    <dbReference type="EnsemblBacteria" id="AGZ39998">
        <property type="protein sequence ID" value="AGZ39998"/>
        <property type="gene ID" value="AFR_08545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1738"/>
    <dbReference type="eggNOG" id="ENOG5032SSX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1376800_0_0_11"/>
    <dbReference type="OrthoDB" id="2025313at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025403">
        <property type="entry name" value="DUF4129"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13559">
        <property type="entry name" value="DUF4129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4129" evidence="2">
        <location>
            <begin position="113"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="21742" checksum="EEF0890EB107393A" modified="2014-01-22" version="1">MTRVWDEFVATVFDVIAPQTLLLLLLLLAGISGALWYWFPAWVPRRWPRLPRLRMPKLRWPRFRRTRKPKAARKGRKSKPEPVFAPTVVLADGTLLADRLAAEGRYAEAVRQRLRDVVGDLAAAGVVTPLPGTTAAEVAAVVATNRPAMAGSLGRATDLFSEIWYGNRPAQHTHDERMRALTGDVRNRMREGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSQ0</accession>
    <name>U5VSQ0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_01835</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357599.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01835"/>
    <dbReference type="EnsemblBacteria" id="AGZ38656">
        <property type="protein sequence ID" value="AGZ38656"/>
        <property type="gene ID" value="AFR_01835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.371"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_3_4_11"/>
    <dbReference type="OrthoDB" id="1864829at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="17"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12420" checksum="BF12C2C9E541E0CD" modified="2014-01-22" version="1">MENPVESSLGDDGHARVAVLGEIDFSNADEVAQGIRDVIADWSPAEVQVDLREATFIDSTGLGALIEGYRAATEGESRFVVTNPSASFRRVLSVTGLCELFGLTDQDESVALSSATGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUD1</accession>
    <name>U5VUD1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10975"/>
    <dbReference type="EnsemblBacteria" id="AGZ40484">
        <property type="protein sequence ID" value="AGZ40484"/>
        <property type="gene ID" value="AFR_10975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2235"/>
    <dbReference type="eggNOG" id="COG2032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095346_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="SSF49329"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665777" evidence="2">
        <location>
            <begin position="19"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="23148" checksum="7BFAA7869636370D" modified="2014-01-22" version="1">MRLLAVPLLLTAALSGCADSGLPTATEVSPPPGSTPWFVSGAPSPPGPSATPSRSDMGAPMTEGTVTGTFLPFPQGVRAITYDPKVVPAGATAQVVIGRTAKGTSVRLAVTGMVPRRTYGAHLHRRTCTSVPDDAGPHYQHSSDPKAAASPPSVDPSYANPRNEVWLDFTADGQGAGSATAAQAWSFDEIEPPRSLIVHALRTRTEVGKAGTAGARVACLTLATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUG4</accession>
    <name>U5VUG4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PHB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360458.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11125"/>
    <dbReference type="EnsemblBacteria" id="AGZ40514">
        <property type="protein sequence ID" value="AGZ40514"/>
        <property type="gene ID" value="AFR_11125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2266"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_2_1_11"/>
    <dbReference type="OrthoDB" id="1213601at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018080">
        <property type="entry name" value="Band_7/stomatin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01270">
        <property type="entry name" value="BAND_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB" evidence="3">
        <location>
            <begin position="22"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="333"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="39775" checksum="440D0E22FBEE6E39" modified="2014-01-22" version="1">MEVVIGVLIFVIALFAVITLVRSVRIVPQQRHDVVERLGKYKRTLSPGLNLLVPFVDAVRTKVDMREQVVSFPPQPVITSDNLVVSIDTVLYFKVVDPVRATYEIANFLQAIEQLTVTTLRNVIGSLDLERALTSREEINRHLSGVLDETTGRWGVKVTRVEIKAIEPPPSIRDSMEKQMRAERDRRAAILTAEGVKQSQILTAEGDKQSAVLRADGDRQARILTAEGQAKAIRTVFNAIHEANPSQKVLAYQYLQALPQIANGTANKVWIVPTELTRALEGMGGALGGLANMVGDLPSSSVDASAVEREAAEAAEAAAKEAAAVNAQVRAAEAQVTGGDGPKGLPAPDAVPPASALGSADYNGAGEPERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUV0</accession>
    <name>U5VUV0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-like N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11675"/>
    <dbReference type="EnsemblBacteria" id="AGZ40624">
        <property type="protein sequence ID" value="AGZ40624"/>
        <property type="gene ID" value="AFR_11675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2378"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099459_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="150" mass="16789" checksum="E52D7A669686CC76" modified="2014-01-22" version="1">MVRLYALVYAEPPYREGPEQVKHFRTRFTDDAQQPGFSLVTAEDDERLVGAAYGWTMPAGSWWPGADDTDIAAVDKFAVLEWLVDPHYRSKGIGGRLFRQLLQDRPEPLATLAADPRSPARAIYARAGWRQAGTLTLAWGTPMDLLVIET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVG8</accession>
    <name>U5VVG8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gamma-glutamylputrescine oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359147.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06635"/>
    <dbReference type="EnsemblBacteria" id="AGZ39616">
        <property type="protein sequence ID" value="AGZ39616"/>
        <property type="gene ID" value="AFR_06635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1354"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_3_2_11"/>
    <dbReference type="OrthoDB" id="1087129at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="35"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="463" mass="51074" checksum="1D8D69EFCFBAF201" modified="2014-01-22" version="1">MASRALADAVPEPYWLGQAGAPAAAEPLHGVETADLVVVGAGYSGLWTALLAKERDPGRDVIVVEAGTAGWAASGRNGGFCSASLTHGFDNGLSRFPDEMDTLERLGRRNLDEIEAAVARYRIKCDFSRTGELNVATADWQLRELTASHDEAVARGLDVQLLDRDEVRAEVDSPTYLGGVWDRTGCAMIDPARLVWGLRQACLDLGVRLFENSPVDRITTTRAGLRLHTWRGRIDAAHVALATGAHAGLLRRLHHYVVPIYDYALMTEPLTADQVGALGWRNRQGVGDGGNQFHYYRLTEDNRILWGGYDAIYYRGGRISPERDQREATFEVLADHFADTFPQLEDVRFTHKWGGVIDTCSRFSSFFGTAYSGRLAYAAGYTGLGVGATRFGANVMLDLLAGEQTELTELKMVRRKPIPFPPEPVRAGVIELTRWSIARADRHEGRRNVWLRTLDKVGLGFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVN5</accession>
    <name>U5VVN5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chorismate mutase</fullName>
            <ecNumber evidence="2">5.4.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_12930</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.5" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12930"/>
    <dbReference type="EnsemblBacteria" id="AGZ40872">
        <property type="protein sequence ID" value="AGZ40872"/>
        <property type="gene ID" value="AFR_12930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2626"/>
    <dbReference type="eggNOG" id="COG1605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090313_1_0_11"/>
    <dbReference type="UniPathway" id="UPA00120">
        <property type="reaction ID" value="UER00203"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008240">
        <property type="entry name" value="Chorismate_mutase_periplasmic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="SSF48600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01806">
        <property type="entry name" value="CM_mono2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665767" description="Chorismate mutase" evidence="4">
        <location>
            <begin position="35"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate mutase" evidence="5">
        <location>
            <begin position="26"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="203" mass="21669" checksum="FD362E4142201BB1" modified="2014-01-22" version="1">MKPKQGSPSRWRHAGASALTAAGLLIAGATPAQAATDRTPATNGLASLVSLSAERLLLADKVAAAKFGTTTPIEDPVREQQVLDQAAGLATEAGVDTEDTVAFFRAQIEMSKVVQRRLYDLWTRHPELAPAERPDLATEVRPQLDRITVAFIGQLAASDDLRGPTLRCVFSLIGAEAAAERRFHLDAQHENALRGAVRPLCAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVP1</accession>
    <name>U5VVP1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NlpC/P60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_07085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07085"/>
    <dbReference type="EnsemblBacteria" id="AGZ39706">
        <property type="protein sequence ID" value="AGZ39706"/>
        <property type="gene ID" value="AFR_07085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1446"/>
    <dbReference type="eggNOG" id="COG0791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034085_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665389" description="NlpC/P60 domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="NlpC/P60" evidence="4">
        <location>
            <begin position="194"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="32504" checksum="6305FFEB456863BD" modified="2014-01-22" version="1">MVAALAIAISGTTVTAAQAAPSTSELTKKIDKASDQLEDVVESFNKMKLSLKKTKAEEKKLAESVGPAKAALVAAGGQMKTLASSAYMTGNVGTMNVVLEGADNLMDRMSILEQISRSRSRDIAEYTATTQNYTERQAALKATQDKQAAEVKELSDRKKKIEADLKKLYSMRTAAYGSPTETGSKYTGSIPKIAGSAGKAVTFAYNAIGVMYDYGADGPNGYDCSGLTSAAWRAAGKSLPHNAAAQYSATTRISRSQLQAGDLVFYRSNGHVAIYVGGGQIIDASRAGQPVKKRTIDIMTPNGYGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW72</accession>
    <name>U5VW72_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glycyl-tRNA synthetase</fullName>
            <shortName evidence="7">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glyQS</name>
        <name evidence="9" type="ORF">AFR_08110</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of glycine to tRNA(Gly).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359442.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08110"/>
    <dbReference type="EnsemblBacteria" id="AGZ39911">
        <property type="protein sequence ID" value="AGZ39911"/>
        <property type="gene ID" value="AFR_08110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1655"/>
    <dbReference type="eggNOG" id="COG0423">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015515_2_1_11"/>
    <dbReference type="OrthoDB" id="626850at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00253_B">
        <property type="entry name" value="Gly_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022961">
        <property type="entry name" value="Gly_tRNA_ligase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="PTHR10745"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="7"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="208"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="320"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="193"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="203"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="280"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="324"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="459" mass="52833" checksum="D4541FDBE5E45083" modified="2014-01-22" version="1">MPADRIDAVVSLAKRRGLVFPSSEIYGGTRSAWDYGPLGVELKENVRRQWWRTMVQQRDDIVGLDSAVILARDVWAASGHLDAFVDPLTECQSCHKRFRADHLEEAWEHKHGSAPASLSELNCPNCGNKGTFTEPKMFNGLMKTYLGPTESADGLHYLRPETAQGIFVNYNNVATASRKKPPFGIAQVGKSFRNEITPGNFIFRTREFEQMEMEFFVPPGSDEEWHEYWLQQRWNWYRDLGLSESNLRFFEHPKEKLSHYSKRTVDIEYRFQFGGTDFAELEGIANRTDFDLTTHSKHSGVDLSYFDQEKQERWVPYVIEPAAGLTRAVLAFLLEAYDEDEAPNTKGGVDKRTVMRFDPRLAPIKVAVLPLSRNPELSPKARGLADLLRKRWMVEFDDSQAIGRRYRRQDEIGTPFCVTVDFDTLTDDAVTVRDRDTMKQERVSLDQIERYLIERLPGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWK3</accession>
    <name>U5VWK3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14835</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14835"/>
    <dbReference type="EnsemblBacteria" id="AGZ41249">
        <property type="protein sequence ID" value="AGZ41249"/>
        <property type="gene ID" value="AFR_14835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3012"/>
    <dbReference type="HOGENOM" id="CLU_1465260_0_0_11"/>
    <dbReference type="OrthoDB" id="2205037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665419" evidence="2">
        <location>
            <begin position="27"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="184" mass="19187" checksum="7C3DE06923F1BBEA" modified="2014-01-22" version="1">MVLTLALLAGGCGTATPDASPTPASSATSASPSASTPSAGATSSRAAPQPRFADLTQGSTTSVVILHAYDSKNASAVVEPIIFMTGDAYCRTFKIKRTDARCVNHAYVTEESNTKVTVPIDAQAKFFTWEDAQGDVCIDAPEKGGTCPMTAKEFGGWVELNKNSMIAVATEDGVITRMAIVYTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWN5</accession>
    <name>U5VWN5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP/GTP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359686.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09155"/>
    <dbReference type="EnsemblBacteria" id="AGZ40120">
        <property type="protein sequence ID" value="AGZ40120"/>
        <property type="gene ID" value="AFR_09155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1863"/>
    <dbReference type="eggNOG" id="COG3903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_8_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003953">
        <property type="term" value="F:NAD+ nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="SSF52200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="1">
        <location>
            <begin position="9"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="664" mass="71433" checksum="CC547B4B45BC6ECA" modified="2014-01-22" version="1">MGVATGGGRVDVFISYAGPDRPWASWAAQQLERAGMSVELTPWDWSAGDNFVLRMNDALGRANRVLALYSTAYFARDRFTTDEWTAVLAEPPDEHGGRRLVPVRVEKVTPPPILRVVTYRDVFGLPEQRARQVLLEAVAGRTRPSGDVRFPGGDPVTASVGGVRVPGSLPPVWNVRRRNPAFTGRGRELAGLRERLCSGERALVQALHGIGGVGKTELAVEYTHLFGNEYDLVWWIDAEVPELIGEQLTALAAAAGWTEIGDAAVITADVVLRRLQRESGWLLIYDNAETVNAIAALIPDGGGHVVITSRSQQTGGVAAAPISVDLLDRETASRLVRELAPALPEADAGRLAAAVGDLPLALGQAAGLVAETGMSVDEYLDELSANPAELLGEGPTGRYPQSLAAVVTASMRHLSGQDEAAGQVMRLVAVLAPEPVPLSWLTGTADGTLPRPLATVAASTTARRRMLGRIAAFGLARVDTDNLQVHRLTQAVITPTTTSLEIEHADRLLVAAAPGDESDPDQWPMWAALLPHLLARATVTASAALRMTAGRALFYLLLRGEHRTAQALATDWHQRWQQTVGPDDRAVLVAASQLANSLNSLGEHEQARRLHEDTLAGRRRVQGDDHPDTLHAANNLANSLNSLGEHEQARRLREDIAKRRETNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWV2</accession>
    <name>U5VWV2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361537.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15980"/>
    <dbReference type="EnsemblBacteria" id="AGZ41478">
        <property type="protein sequence ID" value="AGZ41478"/>
        <property type="gene ID" value="AFR_15980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3243"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_791525_0_0_11"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="354" mass="38766" checksum="6FBA42D02A198B2A" modified="2014-01-22" version="1">MTQALVRPVRNALRRAAGHRDLRRRTPPVVRYDAALSVRPTIYYLSPDDDVPSGGIRMLYRHVDLLNATGRDAAVLHDRRGFRATWFGNDTRVLSARDVTLGPDDILVVPEFYGPGLGALPPDCRKVVFNQNAYSTFTQVPFEGTGPGAPYAGVNGIEALLTVSEDNAALLRYAFPDLAVHLARVVVDEQVFHPGTRAAGRRLGYMPRRRAAEQEQLLHILRSRGCLDGWELVAIDGLSEAQTAEALRGCAIFLSFSEREGFGLPPAEAMASGAYVVGFTGLAGRDYFDPRFCTPVAEADLLAFALAAEEALRRHEEDPETLAAAGRDAAKQILGQYHEEGLRADLTTFYGSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWY8</accession>
    <name>U5VWY8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_16130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361583.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16130"/>
    <dbReference type="EnsemblBacteria" id="AGZ41508">
        <property type="protein sequence ID" value="AGZ41508"/>
        <property type="gene ID" value="AFR_16130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3273"/>
    <dbReference type="HOGENOM" id="CLU_201567_0_0_11"/>
    <dbReference type="OrthoDB" id="1996607at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="60" mass="6466" checksum="B4AC292FADE64DDD" modified="2014-01-22" version="1">MDDVLLAYSTGRADALAGERNADHAAHPATGADYRMGFLDGRIEIFRMLAGVREILDGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX03</accession>
    <name>U5VX03_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein ompR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359787.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09410"/>
    <dbReference type="EnsemblBacteria" id="AGZ40171">
        <property type="protein sequence ID" value="AGZ40171"/>
        <property type="gene ID" value="AFR_09410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1918"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127113_0_0_11"/>
    <dbReference type="OrthoDB" id="1950078at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="12"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="147" mass="15780" checksum="84FB899160CD6A74" modified="2014-01-22" version="1">MSDSKPKPDKYTVLLYSDDLAVRDRIRLAIGVRPAADLSVEFADASTWEECRILLDTYEIDLMVLDGEASPAGGLGIARQTKDEYADPPPTCVVIARAADRWLAAFAQVDATLVHPLDPVTTGQTVAGLLRGRRNGVIPLGTMGTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXF7</accession>
    <name>U5VXF7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15975"/>
    <dbReference type="EnsemblBacteria" id="AGZ41477">
        <property type="protein sequence ID" value="AGZ41477"/>
        <property type="gene ID" value="AFR_15975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3242"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="348" mass="38838" checksum="E437594FC2EAB7A7" modified="2014-01-22" version="1">MVIPCYNYGHYLQDCVQGVLDQDGVDVDVLIIDDDSPDGSVAVARKIADGDARVRLIEHHVNAGHIATYNEGLAAAEGDYVVLLSADDLVTPGSFQRATALMEAHPEVVMVHGFARTFKDVPPPPRTEMRSWTVWPGAEWVARICRAGGNPVATPEVMLRTSTMHQLGDYDPRVPHAGDFLMWLRAAALGGIGRVNDVDQAYYRVHGNNMHVQQFAGADTDLRQRYESFEIFFDEDGRDMPDADRLRTAARLAVAREALVIACACYETKPSTEEAALAGRLVALAEEIYPPARDSRLRRTYTRLVSRTEQGKGPLVPRSISAFNKRVRQHVEWRRWRRYGVESAVKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXI9</accession>
    <name>U5VXI9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Oxygen sensor histidine kinase NreB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Nitrogen regulation protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_16150</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Member of the two-component regulatory system NreB/NreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxygen, probably at the conserved histidine residue, and transfers its phosphate group probably to a conserved aspartate residue of NreC. NreB/NreC activates the expression of the nitrate (narGHJI) and nitrite (nir) reductase operons, as well as the putative nitrate transporter gene narT.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361587.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16150"/>
    <dbReference type="EnsemblBacteria" id="AGZ41512">
        <property type="protein sequence ID" value="AGZ41512"/>
        <property type="gene ID" value="AFR_16150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3277"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="12">
        <location>
            <begin position="287"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ41512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="379" mass="39714" checksum="EFF64E9CF9944FF1" modified="2014-01-22" version="1">MEADSRVRGWRLLAATAGLTVLVVVAAVTSPPGRVRLDLLSSVVLVLPALATAARARFPVAVLAVATLGLVVYQVRGYPGVAPALTVMVALFATVRAGHRWAAGVAVAVSLLGGFAGELAAPGDPDQGVFQRWLLLVGWLLASCVAAELSRQRRHQLGLVEERAATAERTREETARRRADEERLRIARDLHDALTHSISLINVQAGVAVHLARKNGESVPEALVTIQETGREAMRELRATLEVLRADDTAGGAGLDRLDRLVEGARKAGLPVTVTVNGLPRDLPAPVDRTAYRIVQEALTNVTRHAGPASAAVRLDYGECELSVRVDDDGTGSGAPGGTGVGLVGMRERVAALGGSLHAGPRSGTGFSVRATLPMEAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY35</accession>
    <name>U5VY35_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17070"/>
    <dbReference type="EnsemblBacteria" id="AGZ41692">
        <property type="protein sequence ID" value="AGZ41692"/>
        <property type="gene ID" value="AFR_17070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3463"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="13646" checksum="E184508219A1F86D" modified="2014-01-22" version="1">MGVTYYHMVVGEVDLDQVFHALADGTRRDIVVRTLRDEYSVSALSRLYPMTFAAVQKHVAVLERAHLVTKRRSGREQLVTGNVETIRHAARLLDQLEIVWRDRLDRFGDLLAEEDGGDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY59</accession>
    <name>U5VY59_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40566">
        <property type="protein sequence ID" value="AGZ40566"/>
        <property type="gene ID" value="AFR_11385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2321"/>
    <dbReference type="eggNOG" id="ENOG502ZPP7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_568193_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="393"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="480" mass="52920" checksum="94B6E5FD243AC5B9" modified="2014-01-22" version="1">MRERYAPGPLTGPDTARSDEQFSAIDLFAHLLVDGMHVTRVGEDVRVTGDMLVNDVTIEVSLEALQRLRQLPDEGKVVIPILRRKELMLESFEARDGADNRLAHLPQHLTDGLLAWAIKGLFQLAYAPDHELTTTQNSKLYRLIGLICRTDIIDPAEFEVAYTAIVGNTGGTGADDPQLLRSICGYFAGNTVSAVEVDVAEAEQIYVRFQDITTNNRLASSHDRHRTRLGIRPYRYRIPINLAYYAHIYDLSVTGHESHFVFKHYLVESAGQTVNEVLPSHLATEPGMGLRLDRNQGIPHTGLHIRGLNRAVARNLECVAEFEEIPPGALRRTLVISAVCSFVMLAFAFAMPNAVSDSKGTDLAALLLAVPGFAATLVGISTDRVQQSSLTTFIGLVVSASISFAASLLYVLQTLLWRESPQLRLSILGVVKLPSGDVVWMVFAALSICTTVYLTVEGTYRMHRYLAALRRRLTPSNAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYA8</accession>
    <name>U5VYA8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SyrP-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18500</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362351.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18500"/>
    <dbReference type="EnsemblBacteria" id="AGZ41978">
        <property type="protein sequence ID" value="AGZ41978"/>
        <property type="gene ID" value="AFR_18500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3754"/>
    <dbReference type="eggNOG" id="COG2175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044153_0_0_11"/>
    <dbReference type="OrthoDB" id="1742732at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD" evidence="4">
        <location>
            <begin position="12"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="33243" checksum="8DFB12B7F7CF74F3" modified="2014-01-22" version="1">MSTLEATGDTDWLAAHRDKIRSRAAIEGWVRIRGLPITDRAQAAAVIRTLTAGPLDEREGFAPRENHGAGVHSSSEWPPDQPMCMHNELSYRAEVPRQLVLACVTPPVSGGITALADTRAVLAGLPADLVRRSTATGWRLLRSYTGVVGISWQDAFGTTDREAAQAYCAEHGITTTWDADGTLRTVQQRPAVVRHPVTGEDCWFNQLAFLNEWTMDPDVREFLVAQFGSDGLPFTTAYGDGTPLDRDTVDTINEVYEKHTVREPWQRGDVLVLDNILTAHSREPYRGAREILVGMSDPVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYH5</accession>
    <name>U5VYH5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_17770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362137.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17770"/>
    <dbReference type="EnsemblBacteria" id="AGZ41832">
        <property type="protein sequence ID" value="AGZ41832"/>
        <property type="gene ID" value="AFR_17770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3604"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_3_4_11"/>
    <dbReference type="OrthoDB" id="2004291at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="108" mass="11138" checksum="9ADD777EB84D7165" modified="2014-01-22" version="1">MPKDAVVTYEQADADGLVIASLSGELDLDRADAVRDSLAEAASDPTCRYLNVDVAAVTFIDSYALGALVSARNTAASRGVSFTLANPSGPVRKAIQVTGLSHVFGLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYL2</accession>
    <name>U5VYL2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360869.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12620"/>
    <dbReference type="EnsemblBacteria" id="AGZ40810">
        <property type="protein sequence ID" value="AGZ40810"/>
        <property type="gene ID" value="AFR_12620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2563"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_4_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="128" mass="13752" checksum="8A380BD60AEED3D2" modified="2014-01-22" version="1">MHNATGDQAGKHGLALREYIVLSALHKTPGLTQGELGRALSLDKSTLMSRLDRLELKSLVVRRNAPGDRRLRMPEITARGEILRAAVAEASAAAEASVLDGFSPDDVASLRRMLFAVIGDSEDRGSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYN0</accession>
    <name>U5VYN0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18070</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362258.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18070"/>
    <dbReference type="EnsemblBacteria" id="AGZ41892">
        <property type="protein sequence ID" value="AGZ41892"/>
        <property type="gene ID" value="AFR_18070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3667"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_15_10_11"/>
    <dbReference type="OrthoDB" id="1745184at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039538">
        <property type="entry name" value="BetI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13977">
        <property type="entry name" value="TetR_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="20615" checksum="E9D4A1B2B8D1E9B9" modified="2014-01-22" version="1">MPKQVDHQARRTQIADALVRVAAEQGLEAVSLRHVAAAADVSTGMVQHYFRTKDEMMGFAMAVVRERNQERITGRMSRLGPEPGPRELLRTLIAGVLPLDDESRAYGRVALAFLAYTAVRPAAGDPLRKDTEELLGFVTDLIRAGGKASDPAGAAAGLLAVMEGLGVHLLAGHFTPERAIGALDTHLDLLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYP2</accession>
    <name>U5VYP2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molecular chaperone DnaK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360840.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12475"/>
    <dbReference type="EnsemblBacteria" id="AGZ40781">
        <property type="protein sequence ID" value="AGZ40781"/>
        <property type="gene ID" value="AFR_12475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2534"/>
    <dbReference type="eggNOG" id="COG0443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052973_0_0_11"/>
    <dbReference type="OrthoDB" id="1172183at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="351" mass="36020" checksum="F33AA89B1D7EDBAA" modified="2014-01-22" version="1">MPYLLGIDIGSGCTRAAVTRPGGGRDARGPLWEPVSLGVRTRTVPSALATTSDGAAFPAETERDMAIAVGGYLARVGDSLPMQFGGTHYPAHGLVAAMTRWVVDQVWQLLGETPERVALACPTGWGAGRLRLVHAALAEADLAGTALVTRARAVVESFQAAGRVSASGGMLVVYRLGAASVEISVVVPHGPGRVELLGSAEIDEISGYELDGTDPGHAREVLRSTVDLAVHTVRACAGVSGEVAAVLIAGDSGSVHPIVTDLLSAAFSVPVLVDHDPEMTVAGGAALAARPQPRVVPVPPSSVPPPGNAHGASALVGATPSWPEVADGRRVVPERPPRPPVRVAPVRTESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYS5</accession>
    <name>U5VYS5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362310.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18295"/>
    <dbReference type="EnsemblBacteria" id="AGZ41937">
        <property type="protein sequence ID" value="AGZ41937"/>
        <property type="gene ID" value="AFR_18295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3713"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058777_0_0_11"/>
    <dbReference type="OrthoDB" id="1478083at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="23"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28933" checksum="99B07B48579E369A" modified="2014-01-22" version="1">MKRFSLNQATTKHWPLPDLVNGCVEAGVTGVGLWREEVQAYGVERTAALVRDAGLAVTSLCRGGFFQEPGWQDENRRALDEAATLGAPSLVLVSGGLPPGSRDLDAARAHVGRAVGTLVPHAQDVGVQLAIEPLHPMFGSDRCVVSTLKQALDLASPYPSEAVGVVIDTYHLWWDDTVWDQITRAGRENRISCFQAADWVTPLPAGVLLGRGLPGTGCVELRRFREAVDAAGYTGPVEVEVFAENVWSRPGREVLDETIQRYLTHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYU9</accession>
    <name>U5VYU9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13095"/>
    <dbReference type="EnsemblBacteria" id="AGZ40905">
        <property type="protein sequence ID" value="AGZ40905"/>
        <property type="gene ID" value="AFR_13095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2659"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="26572" checksum="7944386A5E4ABF4E" modified="2014-01-22" version="1">MLVLLHGGGPGVDAAGNWRTVLPRLAERFECVTPDLLGFGTQIGPAPDREWPHGPAAWAQERADQILRLLDSQGRRQVHLLGNSAAGGAAALALIASAPERVDRAVVMGGAGTGPLPARVPFYDEPTRDSMRATLGRLVADEKDHLELLAQLTDIRYEQALRPGAEAAFRAMFDAGPGHPAPVDPAAVTRPVLALHGERDRVAPTDVSERLVAALPQARLEVVAGAGHWIHVDRPDEFCDNVERFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ17</accession>
    <name>U5VZ17_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362590.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19730"/>
    <dbReference type="EnsemblBacteria" id="AGZ42218">
        <property type="protein sequence ID" value="AGZ42218"/>
        <property type="gene ID" value="AFR_19730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4003"/>
    <dbReference type="eggNOG" id="COG4106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047561_0_0_11"/>
    <dbReference type="OrthoDB" id="1739932at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="41"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="27838" checksum="60430EB4DBFF0001" modified="2014-01-22" version="1">MTGEFSSTWLTLREPADAAARAVELVEALRLPAGGELVIRDLGCGTGSMGRWLAPRLPGPQRWVLQDRDPALLAHAAAHLPAAAADGSPVTVETAEGDVTALTVEDLAGTSLVTCSALLDLFTTDEVAALAEVCVASGTPALFTLSVVGEVTFEPEDPLDAEVAAAFNAHQRRAVGDRRLLGPEAVGAAAEAFAKAGATVGVRPSPWRLGPGEAQLTAEWLRGWVGAAAEQRPDLRLDAYLEGRIEAAEAGRLQVTVGHQDLLAQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZQ0</accession>
    <name>U5VZQ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14195"/>
    <dbReference type="EnsemblBacteria" id="AGZ41121">
        <property type="protein sequence ID" value="AGZ41121"/>
        <property type="gene ID" value="AFR_14195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2880"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_18_2_11"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="193" mass="20332" checksum="096F0965575D1C7D" modified="2014-01-22" version="1">MSVSPVPEFVAFSPAGPGDAGRIAALHADSWRRHYRGAYADAYLDGDLLTERTAVWSARLASPAGTATILAESGGRLAGFVHVVLDADPRWGSLVDNLHVEHSVHRSGIGTELMAYAARAVLARAASPALYLWVLEQNTRAQGFYQACGGVRVEKGIAAAPLGDPALLNGSPGKFRMSWPDATRLASATAPPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W054</accession>
    <name>U5W054_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GGDEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15390"/>
    <dbReference type="EnsemblBacteria" id="AGZ41360">
        <property type="protein sequence ID" value="AGZ41360"/>
        <property type="gene ID" value="AFR_15390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3125"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_555084_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="362"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="491" mass="52413" checksum="6BD3DA3231DD4013" modified="2014-01-22" version="1">METTKRPGWDTLGLMVLLVLGIIALAAIPLSGASDSLVFVTITGFSQLVFATLALGFAVRTARLKTLTPRVRRAWLLMLPSYALWPVTIVLYGIFPGQEFPSPPDVLRLLIPPATLIGILSFARIPAAPAERAKLRLDAGLVGVGSAMVLWYLMVSPALADSSVGWRELVPSVIHPLTGAALLFGIGVVLMRGPQAAASRRPLLAIVAATLLILTGDAVRAYTLNSGGPVIPTVLQSSLWTGGVFVLMLAPYVQAWYARRPEASRSGTDLRATPRPPRWPYLTVLPGYALLLITVGIRDPFPAGGLIVGALIVSTLAFARQMVSAQESRRLAVTDGLTGLANRVQLYEALPRALARAGRNGTRVGVLIIDMNGFKQVNDTLGHQAGDQLLIGFGELLRRCVLGSDLVARLGGDEFTVVLPDISDEVQAQAVIRRIRAAMAEPIDVGPTMVQPNASIGMAISAPGELAMDELLHRADLAMYETKNSRNAVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0E3</accession>
    <name>U5W0E3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lactamase_B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363059.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22090"/>
    <dbReference type="EnsemblBacteria" id="AGZ42688">
        <property type="protein sequence ID" value="AGZ42688"/>
        <property type="gene ID" value="AFR_22090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4478"/>
    <dbReference type="eggNOG" id="COG2220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1026444_0_0_11"/>
    <dbReference type="OrthoDB" id="767622at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="42"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="270" mass="29385" checksum="45014D43EDF9326A" modified="2014-01-22" version="1">MPQQAKAPPTQPFGADAFAPSDDTILRWLGMAGFLINSRGTTMMIDPVLGGFDMPVMIEMPVAAAEVPHLDAVLVTHSDNDHYSVPTCRDLAKVTDAHQSTRYVAGLMTEHGLPAHGHDIGDHFTVGAVRVQVTPADHAWQNESPGAATRVFRPEDACGFWITTPDGVIWAPGDSRLVPEHHLTRPAPDALLFDFSDSDWHFGLAGAVRMANAYPGTPLLLHHWGSVDAPDFPPFNGNPQDLYDLVENPRRIHVLAPGRPYRLHHSSAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0P3</accession>
    <name>U5W0P3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361591.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41516">
        <property type="protein sequence ID" value="AGZ41516"/>
        <property type="gene ID" value="AFR_16170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3281"/>
    <dbReference type="HOGENOM" id="CLU_951976_0_0_11"/>
    <dbReference type="OrthoDB" id="1897019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665420" evidence="2">
        <location>
            <begin position="30"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="31866" checksum="F3ADDAC10B4DB5ED" modified="2014-01-22" version="1">MITDKLVPRVVAVLAVGLAAAGCANGGAADTSTDGRQALTQSLQGLQTGNYTFTRTGGRAAEGSVHLPESSMISDAYGPTVLRTGRDFFMKYRIHGEGHEQYGKLYDQYAGKATTKKEAAELRKAKEILRVLDGTRWVRADEKRLVTAAAEDDLSGMENLPPTPTAAKADITGATALIDAAVTAKLSGSTITGTLDATKVDPELGILTNDPYYVYGPRAKSMTFKATVDDGGRLTQLTVDLPGPLQDAASQPPEGFPSDAPTTAAEPPLTITVSAYGETAVQGPPEGATDLDPAAYEMLTNDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Y9</accession>
    <name>U5W0Y9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_23290</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363474.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23290"/>
    <dbReference type="EnsemblBacteria" id="AGZ42928">
        <property type="protein sequence ID" value="AGZ42928"/>
        <property type="gene ID" value="AFR_23290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4720"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014629_4_0_11"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="PTHR10909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="8">
        <location>
            <begin position="133"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="276"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="ACOX" evidence="7">
        <location>
            <begin position="498"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="643"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="663" mass="72567" checksum="B4F279FC7E28A9AB" modified="2014-01-22" version="1">MKADSVDPARVQDVLDGRWGHVRRAARTQLAGDHFVAVPGETLEQARERITRLLRELPTDLGAAAMFPDEHGGAFEPGAAVTAAEMLAQLDLSLMVKAGVQWGLFGGAVAALGTRHHHETYLKEIISADLLGCFAMTETGHGSDVHRLRTVCEYDPATGTFDLHTPHEAARKDYIGNAARDGRMAVVFAQLVTQGEGRGVHAFLVPLRDDDGAALPGVTIGDDGHKAGLLGVDNGRLSFDHVAVPREMLLDRYGQVAPDGTYTSPIDNDARRFFTMLGTLVRGRVTVGGAASAATRQALTIAVRYGETRRQFTAPGQDREVVLNDYLAHQRKLLIALARTYAFTFAQEELVTTLQEVQTADGPVDEHRQRELESRAAALKAAQTWHATRTIQTCREACGGAGYLAENRLPGLKADTDVFTTFEGDNTVLLQLVAKGLLTGYRDNFESLDGWGRGTFVAEQVREMVLERTAARSLIRRLVDAAPGGSDAGAFTDRGWQLKMLEDREKHVLEGVIRRLRKGAATKKDRPFDIFNDVQDHVLEAASAHIDRVVLEAFVAGIERATDPAARELLARVCDLFALSTIEAGKGWFLEHERLTPGRSKLLTSAVNGLLKELRPHAATLVDAFGIPDAWLACAILREEPGRQEEMARDDQRRDSRRSTNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W190</accession>
    <name>U5W190_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative HTH-type transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_16915</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16915"/>
    <dbReference type="EnsemblBacteria" id="AGZ41661">
        <property type="protein sequence ID" value="AGZ41661"/>
        <property type="gene ID" value="AFR_16915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3432"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="16828" checksum="15FEE34DF6A69E82" modified="2014-01-22" version="1">MASALDDIDRQILAELTRDGRMSMRTLAETLHISRANAYARVERLRAANVIRGFRADIDPVAAGLGTSAYVTVNLHQAEWRVVGEQLRALPGVVHIALVGGEFDAILLVRAKDNADLGRLVLNVIQGMPGVINTRTLLVFEETEAPMGAVTTLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1F3</accession>
    <name>U5W1F3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FtsX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_17315</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17315"/>
    <dbReference type="EnsemblBacteria" id="AGZ41741">
        <property type="protein sequence ID" value="AGZ41741"/>
        <property type="gene ID" value="AFR_17315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3511"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089207_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="125"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="22436" checksum="696393DD7347F985" modified="2014-01-22" version="1">MWLGGLWFSVAGILAPVPLAPELDSAALVGWTTAARLLDFDGSPTTVYTRSVEQSVEEVRQVLAATVNPQAPNEVQVSRPSDALAAARATDAALNGLLLGLGAVALLVGGVGVANTMVISVLERRSEIGLRRALGATGGQVSVQFLMESLLLSALGGGAGVLCGVLATAGWSWWRSWPVAVPLWALAGGFTATLVVGALAGLYPALRAARLSPTEALAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1J1</accession>
    <name>U5W1J1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">von willebrand factor type a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17835</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17835"/>
    <dbReference type="EnsemblBacteria" id="AGZ41845">
        <property type="protein sequence ID" value="AGZ41845"/>
        <property type="gene ID" value="AFR_17835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3618"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018489_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="360"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="557" mass="56988" checksum="E5A38E54376CD3E2" modified="2014-01-22" version="1">MVAGVRVGINQFNGAECSGEVHLTVAAAPGLAEAVRSEADAWTGEKAEIDGSCIRIDVQSSDPADVAAVLSAKQRVSLTGAGAPREGVAAPDVWISDSRSWLQRLRSAAPSFAFTEEGSVALSPVVLAMPAPIAESLGWPRKKLTYNDLLTLITTSTTTRTGTADPTRDATALSGLLALTAAATAADQRKPGTSNGLLRSLATDTSVLRDDLMAQLPQSADDAALAAGLSLAAMPESDVVRYNAGNPPVPLAALYLEPAPVPLDHPFAVMPEISPDQAEAAHQFYGRLTTGSAFRAQLGTAGLRAPDGTAPSGLKRPEGAPARIDTGASAGSGEAAASDADGASIDRALAGWSAVIAPARMLAIVDSSTSMRTPVPTARNATRMQLTIAAARGGLSLFSDDWAVGLWQSAGGSGGGHSELVPIRPLTGNRTTVANALAGIRPSSGEAGLYRSILEGYRAVQNGWQAGRVNSVLVLTDGVGIGEGDLELNDLLDQLAEAKTADKPVQVIVLGIGNAVEQPPLKKITGVTGGGVFVAEDPAQIGAVFLEALSLRTTAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1P8</accession>
    <name>U5W1P8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methylisocitrate lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23410"/>
    <dbReference type="EnsemblBacteria" id="AGZ42952">
        <property type="protein sequence ID" value="AGZ42952"/>
        <property type="gene ID" value="AFR_23410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4744"/>
    <dbReference type="eggNOG" id="COG2513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027389_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="27237" checksum="93E53C4065A83EFD" modified="2014-01-22" version="1">MISTMTAADLRALLAADHVTHAPGVHDPASAALAVRAGHRAVHLSGQAIAATMLGRPGLGLTPSTQIADRAALLGAALEGVPLLADLDVAFDAPGDVVWTALAYQRAGISGLFLGDGEDIGLSASRVTSLVRQAPGVAVIAQARGNRTIERCRAYAAAGADAVLPTGIAERDLGRLRDALPGVPLVVNRSEAGAGDGRLTDAELAGLGVRLVLHPLAALLAALRAASLAYRAILEEGSAERVDRLPLATFATLTETLPVAVTDRIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Y9</accession>
    <name>U5W2Y9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator, XRE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20095"/>
    <dbReference type="EnsemblBacteria" id="AGZ42291">
        <property type="protein sequence ID" value="AGZ42291"/>
        <property type="gene ID" value="AFR_20095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4076"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085376_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="178" mass="19566" checksum="9F3F056A7F084F02" modified="2014-01-22" version="1">MAARVAELRVVRGWSLDDLAGHTGVSRSTLSRLERAEISPTASLLGKLCTAYGWTMSRLLAEVETEPPAVVRVADQQIWRDEASGFTRRSVSPPHPGLRAELIEGELAPGADITYERPPVTGMEQHVWVLGGRLEFTDNGTVHVLETGDCLRFRLWGGTRLRNPGDEPARYAIVVVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W302</accession>
    <name>U5W302_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_26235</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26235"/>
    <dbReference type="EnsemblBacteria" id="AGZ43509">
        <property type="protein sequence ID" value="AGZ43509"/>
        <property type="gene ID" value="AFR_26235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5317"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4783">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011707_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031634">
        <property type="entry name" value="PknG_rubred"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031636">
        <property type="entry name" value="PknG_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16919">
        <property type="entry name" value="PknG_rubred"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16918">
        <property type="entry name" value="PknG_TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="148"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="642"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="734" mass="78605" checksum="164F7CE71A2823E3" modified="2014-01-22" version="1">MNCQRNCPGTIQDGYCDTCGMAPAASAPSPSPSPKQRTATTPSVRTGALSTRTGGTARTGSARTGSRRRRFGGGLVDVAPLPERDPATAIMTVAEVPEDKRYCAKCGNAVGRSRGDRTGRTSGFCPSCGEAFNFTPKLVKGDLVGGQYEVIGALAHGGLGWIYLANDKRVSDRWVVLKGLLNSGDEDALAAAVAEQRFLAEVEHPNIVKIYNFVEHEGAGYIVMEYVGGESLKGILKDRRTANGNQADPLPLDQALAYVLEIMPAFGYLHDRGLIFCDFKPDNVIQTGDQVKLIDLGGVVHIDDQDAALYGTVGYQAPEMAQAGPSIQSDLYTIGRTLAVLTTDFRGYQSTFKESLPEHGDFEVYQRHESFYRLLHRATRADPAERFVDAGEMTEQMLGVLREVLAADGEPRPSTSRLFTGELRTDPRSDEPNWRFLPAPMIDLTDPSAAFLASVTVTEPQEVLTLLGKAPQNTFEVQLRALRALIDLGAARRTPDAFTDAVRLRDTIAAAAGGDWRLAWYDGVLALASGDLARAQAQFDAVYSALPGELAPKLALGLTLELAQRPAEAAGYYDVVSRTDPAYTTASSGLARCRLATGDREGAVEAYNRVPGTSAAYRSSQVGAVRALVRSHASAAADVASLAAAAELIDRLEMEAAQLAALRAELLERALTTLKAGTAVPSAVVGTTGGGLAGERDVRFALEDAYREMARAVHGPERIRLVDLANASRPRTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W346</accession>
    <name>U5W346_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydroxypyruvate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_20740</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the hyi family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20740"/>
    <dbReference type="EnsemblBacteria" id="AGZ42420">
        <property type="protein sequence ID" value="AGZ42420"/>
        <property type="gene ID" value="AFR_20740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4205"/>
    <dbReference type="eggNOG" id="COG3622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050006_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026040">
        <property type="entry name" value="HyI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006241">
        <property type="entry name" value="HyI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="3">
        <location>
            <begin position="38"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="257" mass="27385" checksum="20C982A10EF10E23" modified="2014-01-22" version="1">MCAEMVFLDLPFEERVRRIADLGFEVEIWDWTRKDITALRSTGATFSSMTGYVTGTLADSDGADELVRTAELALEVADRLDCPRLNLHGTGLDGSGLPVVPCATPTPAMWLTAARTLTRIAGLGERAGRVFTLENLNTAVDHPGTPFARAADTMTLVAAVGSPHLRMNLDLYHAQIGEGNLIALVRAALPLIGEIQVADVPGRCEPGTGEINYRNVARALEGYDGVVSLEGWASGDSGAALERFREAFTSGASPRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W348</accession>
    <name>U5W348_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SGNH_hydro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20420"/>
    <dbReference type="EnsemblBacteria" id="AGZ42356">
        <property type="protein sequence ID" value="AGZ42356"/>
        <property type="gene ID" value="AFR_20420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4141"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059193_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14606">
        <property type="entry name" value="Lipase_GDSL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH_hydro" evidence="1">
        <location>
            <begin position="173"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="396" mass="41619" checksum="9919437F88657CB1" modified="2014-01-22" version="1">MPDLITTPLTAGLVRGALELEPTEHGLLPHRLPARARAQCADPQLLMAESQPSGVRLAFRTRATTVELDTLPTRRVYAGAPPRPDGVYDLVVDGKLSGQASVDGGNTLVIDMATGAAESRTGPVGTVRFTDLPAEDKTVEIWLPHNETTELVALRTDAPVEPVQEQGRVWLHHGSSISHGSNAASPSTTWPAIAASLGGVSLINLGLGGSALLDPFTARALRDTPADLISLKIGINIVNADVMRLRAFGPAVHGFLDTIREGQPTTPLLVVSPILCPIHEDTPGPAAVDVEAMSRGELHFRATGDPAERAAGKLTLKVIRAELARIVAERATDDPNLHFLDGLGLYGPADADELPLPDDLHPDAATHRRIGERFAGLAFSAGGPFAAQVVGGSSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3L0</accession>
    <name>U5W3L0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21400"/>
    <dbReference type="EnsemblBacteria" id="AGZ42550">
        <property type="protein sequence ID" value="AGZ42550"/>
        <property type="gene ID" value="AFR_21400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4342"/>
    <dbReference type="eggNOG" id="COG4332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090982_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009412">
        <property type="entry name" value="DUF1062"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06353">
        <property type="entry name" value="DUF1062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="187" mass="20970" checksum="38096C3C2C5BE36E" modified="2014-01-22" version="1">MVSEVGLPAIVKACVSCRSTRHRPTGKIRVNANHKLLDVWLLICCELCGRTSKVPVHERVNVRALDHERLVKFHDNDPAVVRQLTMDATLAQRAGYQLDWTGTWRLDTDMPFYDLQRGDAAPHEVIVRFELPAPVRVEKLLTAGFGLSRPAVRGLVDSGRIRLPLPVYAKAREDFTFVVVTASPEHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3M7</accession>
    <name>U5W3M7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein translocase subunit SecA</fullName>
            <ecNumber evidence="11">7.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">secA</name>
        <name evidence="15" type="ORF">AFR_26775</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="11">Distribution is 50-50.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the SecA family.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.8" evidence="11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364491.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26775"/>
    <dbReference type="EnsemblBacteria" id="AGZ43617">
        <property type="protein sequence ID" value="AGZ43617"/>
        <property type="gene ID" value="AFR_26775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5428"/>
    <dbReference type="eggNOG" id="COG0653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005314_3_2_11"/>
    <dbReference type="OrthoDB" id="212453at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18803">
        <property type="entry name" value="SF2_C_secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026389">
        <property type="entry name" value="SecA_Actinobact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020937">
        <property type="entry name" value="SecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="PTHR30612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="SSF81767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="SSF81886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04221">
        <property type="entry name" value="SecA2_Mycobac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01312">
        <property type="entry name" value="SECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-1278">Translocase</keyword>
    <keyword evidence="9 11" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SECA_MOTOR_DEAD" evidence="14">
        <location>
            <begin position="1"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="84"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="411"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="100"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="489"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ43617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="762" mass="83429" checksum="F9A09DDE1CE39C39" modified="2014-01-22" version="1">MGVSQRFKNRFRKFLQRPGTTVDLAPLEKRLPDIEAREEALRELDDAALTEAAGAAKDYTEICAVGREAAWRALEQRPYDVQLVGAMALLSGKVAEMATGEGKTLTATIAAYGHVLLGNGPVHVLTVNDYLARRDATWMEPVYTLLGLTVGWVTEASTPEERRAAYAADVTYVSVSEGGFDYLRDQLVTDVEDRVQRELATCIVDEADSILIDEARVPMVLAGSIATESDPVHDAAELVRDLRPGKDYEVAEDGRSVAFTNAGLAALEEKMGIDLYADDQVAQLSAVNVALHARALLRRDVDYIVRDGGVELIDEMRGRVAQRRRWPDGLQAAVEAKEGLTATAEGEVLDTITVQAYIALYKTVCGMTATAVHVGEQLREYFKLEVAVIPSNTPNIREDEPDRIYSAHDMRDEALVEEIKIAHDKGRPVLIGTLDVKASELLAKQLAAAGVPSVVLNAKNDDEEASIIAEAGALGAVTVSTQMAGRGVDIRLGGSDGKDAEKVAELGGLFVIGSGRHDSRRVDDQLRGRAGRQGDPGGSVFFVSLEDDLITRHAGDILPSSPRMDMDGVVHDEQVDYAVEHAQRVAEGVNHEIHRNTWRYSVVIEGQRLALAERRERLLTSEIAAIMLLERSTEKAKEIDEDVLSEAARSIALYHLDRLWAEHLAELSEVREGVHLRALGKLDPLDEFHRSAVPAFQKVFTEIESRTIATFEELDLSDGYHPDNAEIVRPSATWTYLVHDNPFGSELDRLIAAVGRRLTSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3X9</accession>
    <name>U5W3X9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560176.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27890"/>
    <dbReference type="EnsemblBacteria" id="AGZ43839">
        <property type="protein sequence ID" value="AGZ43839"/>
        <property type="gene ID" value="AFR_27890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5652"/>
    <dbReference type="eggNOG" id="ENOG502ZA6T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087930_0_0_11"/>
    <dbReference type="OrthoDB" id="1602348at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="255" mass="27664" checksum="9B9596E4A83F66FD" modified="2014-01-22" version="1">MAYPRGLTDDVRTDWGDLGEATGKPAVFGEGDLSALPEPVRRWLTHSIAPETPLLTAVEVTTHGTVRDSAWRDFSATQRLSPARGFVWAANGTMAGMPVTGFDRYTRLTGQKRWRLLSRVPVRTADGPDLTRSSAARLAGDLLIVAPAAALSSRVKWQPLDADRAVARIRVDAGYHEVTLTVAADGSLTSMSMPRWGADTRGSFSERVFGADLTDETTFEGFTIPGTVVAGWDHGTAAWADGQFLRYTVDAAHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4P4</accession>
    <name>U5W4P4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363420.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23180"/>
    <dbReference type="EnsemblBacteria" id="AGZ42906">
        <property type="protein sequence ID" value="AGZ42906"/>
        <property type="gene ID" value="AFR_23180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4699"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_0_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="35"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="294" mass="32551" checksum="F960057C2F364ED7" modified="2014-01-22" version="1">MATEHPYARELGDFLRAKRGQLRPEDVGLEPGGRRKVAGLRREELALLAGLSTDYYQRMEQGREMRPSEEVLDALATALDLDDEERRHLVTLARAARRPVPARTRRAPERVPESTRRMLQIMATPALVLGRHLDVLAWNPLAAALLGDPDTVPAGQRNMLIAMFTDPEARERCAGWEDFAVEYIGMMRAAVAADPTHPRATTVIGELSIHSADFREMWARHDVREAVSGRKTFRHPAVGDITLDWDTYPLPGSPGPVLLVYTAEPGTPSADRIQLLASLQATTSQRLAGYPPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Y5</accession>
    <name>U5W4Y5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23690"/>
    <dbReference type="EnsemblBacteria" id="AGZ43006">
        <property type="protein sequence ID" value="AGZ43006"/>
        <property type="gene ID" value="AFR_23690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4800"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2535036_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="83" mass="9022" checksum="C946A164CF07DEEF" modified="2014-01-22" version="1">MSLGGQEYVIEPGDYLFLPRNVVHTFRNSADVEARVISVVSPAGLEAYYQALAELPPGPKDIATIQKIMVEFGIELQLPPGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5N2</accession>
    <name>U5W5N2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24825</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24825"/>
    <dbReference type="EnsemblBacteria" id="AGZ43231">
        <property type="protein sequence ID" value="AGZ43231"/>
        <property type="gene ID" value="AFR_24825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5029"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666103" evidence="2">
        <location>
            <begin position="17"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="364"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="3">
        <location>
            <begin position="504"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="767" mass="81048" checksum="AC52B8809B0926B0" modified="2014-01-22" version="1">MGRRRAQKRARPGILPVAAALLAVAAVVVWLLVRQDGSVMPGYAGGPVAMLAAFVACRRIGSMIVVARPIRGFWRLFSQSAICVGIGSVGSFVFANNDPGLSPYMAVPTLVGVVMSVLAFFQLPVERRSGLRWTQLILDGATVAVAGALIYWYVVLDMADPSISPVTQTGAAVVGVGALISLVVIGRAAMSPASTVDSVSLRILAVAPLVAVATTVLLIGGGETGRLIISVLGVPVVGAAMALAAYRQQVVLGRPAAAPAAPKARSIYNALPLVAVIATAALVITVSARQLGWHQRVVIIGAVLIAAFVVARQLLGLRANDKLLVGIRRQQAELEHLAMHDPLTGLANRARFGAVLDERLDAHRPAAVLLVDIDDFKMVNDTMGHAIGDQLLYEVALRLRNHSTVNHLPSRLGGDEFAVLLDTDEAGPAEDAAGRILAALAVPFKVGEHQLLANASIGVALAGPGDGGDEVLRNADIAMYAAKAAGKAAWARFEPRMRNEVVNHARLGSELHNAIIRHELFLAYQPVFDIVTGAMSGAEALVRWKHPVRGNVPPGDFIPVAERSGLIVPLGSWVLREACEQLAKWHAEYGSAAIRSINVNVAARQLREAGFVDEVAAVLSDTGLIPANLVLEVTESSVIDGRQVRETLEALHELGVRLALDDFGTGQSSLSLLRAFPVDVLKLDKSFVDGICDGEDRGRLAVAAAVAQLAEYLDLYAVAEGIETAAQLDRLRAMGYRLGQGYYLAKPLPPDEMAALMTQTARTVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Q0</accession>
    <name>U5W5Q0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FtsX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_30360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560669.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30360"/>
    <dbReference type="EnsemblBacteria" id="AGZ44332">
        <property type="protein sequence ID" value="AGZ44332"/>
        <property type="gene ID" value="AFR_30360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6147"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012341_3_0_11"/>
    <dbReference type="OrthoDB" id="1230252at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038766">
        <property type="entry name" value="Membrane_comp_ABC_pdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30287">
        <property type="entry name" value="PTHR30287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="87"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="439" mass="44593" checksum="955374D8A519AFA8" modified="2014-01-22" version="1">MIRQALRRNPWSFLGPATTQFLAAALVAGALAVMTSISRAPLDAATRQAVDDSGLPDMAMVFLMLAIYLSIIIVGVTMNATIAQQARDIALVRAIGATPGRVRRAIATQAAVVAVPATLLGVPLGTVGARAWLDGLVAHGIAPPAITFHAHGGALPIALAVTVGTSVIGALIAAIRPSRVRPAAALAETAAPRRRVGIVRTVVGLVLVCGGIGLSVAIADRSAQVANDLGLFVMLAMCVGAGCLGPALLTVAAPVARLFGDTGRLAADNVLVRARGLSGALVPLTLAVAFAAVKVVSYTTAAHVTGDPGSASDRWLEYSGTGVYTVFAAVAALNTLITMMLARRRDLAVTQLAGGTRRRTLGVVFCEALVVTGTALVVAAGVAVATLLPLLHTALGTWTPWLPPSWLLAGVLGTAALVLAGTWVPAAAALRRPPIEVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5W4</accession>
    <name>U5W5W4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_25630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25630"/>
    <dbReference type="EnsemblBacteria" id="AGZ43390">
        <property type="protein sequence ID" value="AGZ43390"/>
        <property type="gene ID" value="AFR_25630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5195"/>
    <dbReference type="eggNOG" id="COG2340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048111_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665580" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="172"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="30875" checksum="84B5DFB7FF0BEACC" modified="2014-01-22" version="1">MRKPAVVVTAAVSAVLAGGIMVATTAGAEETPDAVTAPSVQWPDLPAIPQLPGGADLPAVPAVPGNPGPAAGHAAPAHQARPDDQAGTADQAEPADDQAQPNDQRPAVIRPARPAAATTPATADAGEDKRNKATQPDKPKTLAAQPGNAKRDVTASQALSTDPRSSVQQQALELVNENRRRGGCENLSVDRRLIVAANKHAADMARRGYFAHEDSQGDRAGERVEDAGYRWKRFGENIARGQDSVYEVVNGWMNSPEHRENIMDCDLHQVGVGLAFSGDRTPYWVQDFATPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5X8</accession>
    <name>U5W5X8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lantibiotic transport ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_30785</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560752.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30785"/>
    <dbReference type="EnsemblBacteria" id="AGZ44417">
        <property type="protein sequence ID" value="AGZ44417"/>
        <property type="gene ID" value="AFR_30785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6232"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="26237" checksum="FF8F7F090D24289A" modified="2014-01-22" version="1">MNETSIVVDGLKKRFGPAVALDGMTFTVPPGQVTGFIGPNGAGKSTTMRVVLGLDRADAGTALVGGRAYRELRDPLRRLGSLLDAGALQPGRTARNHLLWLAHSQGLGAARVDEVIEQAGLGQVARRRAGGFSLGMKQRLGIAAAMLGDPPALMLDEPFNGMDPEGIVWMRGFLRALAAEGRAVLLSTHLMSELQGTADHVVVVGRGRVIADQSMAALLTGRTGLEQAYLELTRDAVEFRATGTETTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W659</accession>
    <name>U5W659_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH-type transcriptional regulator malT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561001.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32050"/>
    <dbReference type="EnsemblBacteria" id="AGZ44668">
        <property type="protein sequence ID" value="AGZ44668"/>
        <property type="gene ID" value="AFR_32050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6489"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3899">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_0_2_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="885"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="950" mass="101974" checksum="15ED3E8902B1C2BB" modified="2014-01-22" version="1">MASEGDDAPLVGRSGTLTAVRTDLLAAGPGGTAAVFVTGESGVGKSRLLRETAEALRSAGAAVLSGTCLDIGDASPLHPVLQALRRAGVHTEPGPADSGTGQDGAGALLERVSRELRSLAQGRRLLLVLDDLQWADRSTRQLLLYLLAGLGELNLSVLAAVRAESLHGSHPLRRVLAELRRLRSVRVLDLAPLDRDATDELVAAIAGADVAPEDADRVYKRSGGNPFVVEELARDLRDGRVELSDTLREIFLSRVDELPPHAHEVVHAVAAGVEPVEHALLARVLPLPETELIEAIRAAVAHRFVTSAADGYRLRHRVVAEVLEHEVLPAESAARHRRYAEAIEASPCGPDHARLAHHWRQAGEPVRALPSVIAAARDAEQLYGYVESHRYWTLALELTGDDDAQRTELLSRAAEAAHRCGEHQRALTRIEELAARPDGPGKCEIHLRRARYLAAAGRSATAEIEYERALAVGECTPAEQASAAAHLAELLVHLGRYADAGVRARDALELSEASDGSTADLVRASAALGFSLAFREDPDAGLAVIRQAVEIAERSGHPDDVGCAYLHLAELLTGPLNSVEEGVLVARRGAEKLAAMGLGRSYQTRLLAIAANGLFRVGDWAEAERVLDTAMRHRPSGADAVELLLSRCRLWVGFGDVEQANRDLDAVATILAGGGARHVLPMLTLRAGLAMWQGRHAEARTAVQRGLTETRSDDLVLLGVLAWHGLRAEAEAQAGGEVPVDPAAVRRLQTVVERMAKGAGNAAPAVRAVVDGYLDLCAAEQSRIDERNDPELWARAAASWDRRKQPYPAAYSRLRQAEASFARRPSRSARSRAAATTALREAYATARAMGAKPFAAEITAVATRARVALTGDEDTVPLRAPDRDTGDELGVLTDREREVLVLVAEGLTNREIGQALFISERTVGVHVGHIFDKLQVRTRVQASRVFLRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W677</accession>
    <name>U5W677_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrilase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364175.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25960"/>
    <dbReference type="EnsemblBacteria" id="AGZ43456">
        <property type="protein sequence ID" value="AGZ43456"/>
        <property type="gene ID" value="AFR_25960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5263"/>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_1_11"/>
    <dbReference type="OrthoDB" id="1583074at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="29242" checksum="0E692AB23452EF19" modified="2014-01-22" version="1">MAVTRIAAAAAHFGRDLDYDLGRIGKLVADAREAGAGLLVLPDAALGGYLADLRRPDPDALPPALEPDHPLFDKIAALARDMVVCVGYCEAGGDNRYNAAVSLTGDGVLGRHRKVHLPAAEVTAYAAGDTFAAFDTPVGRVGMLIDYDKTFPESARTLALDGAEIVACLSAWPASITNQAPKLSQDRQSRLFDLYDCARAAENQIVVVSSNQTGAVGGMRFLGQAKVVGPAGNILARTWAKAGLAVAELDVAAEIAAARRVLRHLDELRPATYRTAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6C8</accession>
    <name>U5W6C8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spermidine/putrescine import ATP-binding protein potA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_32500</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561097.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32500"/>
    <dbReference type="EnsemblBacteria" id="AGZ44758">
        <property type="protein sequence ID" value="AGZ44758"/>
        <property type="gene ID" value="AFR_32500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6577"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="216" mass="22034" checksum="5F8562168161B2E8" modified="2014-01-22" version="1">MRTLEVVAADVAYGDGVPVLRDVTVTVRPGELLAVTGPSGAGKTTLLAAMAGLLRPAAGRVLVDGEELRDRDQGVGLGVVLIPQENGLAAILTAAENVAVAMIATGGSTADARRGTSESLEKVGLAAQADQLIDELSGGQQQRAAIARGLALHGDVLLADEVTSELDAANRQKVIELLRAEAARGAAVVFATHDPEAAAACDHELHLADGDATLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6E7</accession>
    <name>U5W6E7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561051.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32305"/>
    <dbReference type="EnsemblBacteria" id="AGZ44719">
        <property type="protein sequence ID" value="AGZ44719"/>
        <property type="gene ID" value="AFR_32305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6539"/>
    <dbReference type="eggNOG" id="COG5015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139738_2_1_11"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019965">
        <property type="entry name" value="PPOX_F420-dep_Rv2061_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03666">
        <property type="entry name" value="Rv2061_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="13924" checksum="3635128FFA225FCB" modified="2014-01-22" version="1">MISLDTLALQRTVLLETRKRDGSWVATPVSIVADAGRAYFRSYDAAGKAKRLRNFPQVRVAPCTMRGRPTGPAVAGSARLLSDEEAAPVRRMLAAKHPVLHGRLVPALHRRKGWTTLHYELTLEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6G9</accession>
    <name>U5W6G9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31660"/>
    <dbReference type="EnsemblBacteria" id="AGZ44592">
        <property type="protein sequence ID" value="AGZ44592"/>
        <property type="gene ID" value="AFR_31660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6408"/>
    <dbReference type="eggNOG" id="COG2047">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055821_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008492">
        <property type="entry name" value="DUF774"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028754">
        <property type="entry name" value="UCP028754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="319" mass="35190" checksum="A8A8184C06BCC29A" modified="2014-01-22" version="1">MLSTYCPPLRAHLEEVKSVLDPHGLYELADDLPDLDSPVLVQALTGFVDAGSAIQLSREHLLEHLDGRVLATFDVDQLLDYRSRRPSMIFVEDHWESYEEPSLALHLMRDQLGTQFLLLAGPEPDLQWERFIEAITGLIERFGVKLTVGLNAIPMAVPHTRPVSVTAHATDQSLLGDRESWLQRVQVPASVGNLLEFRLGESGHDAMGYAAHVPHYLAQTTYPAAAQLLLDSVSQTTGLALPTGQLREAADLVREEVDKQVADDEQASRLVVSLEAQYDAFLRGRQGNLLADSGGPLPTAEELGAELERFLAEQSRDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6H1</accession>
    <name>U5W6H1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32455"/>
    <dbReference type="EnsemblBacteria" id="AGZ44749">
        <property type="protein sequence ID" value="AGZ44749"/>
        <property type="gene ID" value="AFR_32455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6568"/>
    <dbReference type="HOGENOM" id="CLU_2257654_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="103" mass="10615" checksum="19C88F66B9010781" modified="2014-01-22" version="1">MGALTLGLGAGLGIGMLGGRALGWPALVLLGIGAALLIMSIGDAPRPAEAQPARPARPALAGLGERTEQILKLAEEQAEDHVRQAREEARRIVAEARAGHLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6W7</accession>
    <name>U5W6W7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32445"/>
    <dbReference type="EnsemblBacteria" id="AGZ44747">
        <property type="protein sequence ID" value="AGZ44747"/>
        <property type="gene ID" value="AFR_32445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6566"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078527_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016259">
        <property type="entry name" value="Hygromycin-B_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000707">
        <property type="entry name" value="Hygromycin-B_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="60"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="33934" checksum="A1D34413581ACF27" modified="2014-01-22" version="1">MTRENVDSVWPAADDEEDFDFVARDEEALGPGVRALCASLGVTGAPSRYPAGSLPVYAVGDAVLKLFPPVHVEELPVEAGVLSAVQGRLTVPTPAVHASGTFERWGYVLMDRMPGVPLASVWPDLSPADRDSLAVQLGEAIASLHAVPVPEIEDWWPEDWPEFVEDQVTGCAGRQRALGLDPAWVAQIPSFLAGVPLGEPEPVLLHTEILGEHLLVEDGRLSGLIDFEPAMRGAPEYDFVGPAVFLADGDARFYDRMLRAYGAGPDRELRQRLMAWTLLHYYSNLGAYLERLPPPAEPTFASLADRWFGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W712</accession>
    <name>U5W712_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucanase</fullName>
            <ecNumber evidence="8">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_27300</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase family 6.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27300"/>
    <dbReference type="EnsemblBacteria" id="AGZ43721">
        <property type="protein sequence ID" value="AGZ43721"/>
        <property type="gene ID" value="AFR_27300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5532"/>
    <dbReference type="eggNOG" id="COG5297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015488_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016288">
        <property type="entry name" value="Beta_cellobiohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036434">
        <property type="entry name" value="Beta_cellobiohydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001524">
        <property type="entry name" value="Glyco_hydro_6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34876">
        <property type="entry name" value="PTHR34876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01341">
        <property type="entry name" value="Glyco_hydro_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001100">
        <property type="entry name" value="Beta_cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00733">
        <property type="entry name" value="GLHYDRLASE6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51989">
        <property type="entry name" value="SSF51989"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00655">
        <property type="entry name" value="GLYCOSYL_HYDROL_F6_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 8" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="4 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005147819" description="Glucanase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="10">
        <location>
            <begin position="25"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="126"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001100-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ43721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="581" mass="60183" checksum="D40F07AF61C31A39" modified="2014-01-22" version="1">MTRGLTAAAAITLLSGAGIAVAASQASAAAGCRVTYSVNQWSTGFTGNVSVTNLGDPISGGWNLTWAFGGNQRITQGWSATVTQSGQNVTATSPSWANSLATNASATFGFNADYSGTNATPATFSLNGTACTGTPGGPTTPPPSNPTTPPPTTPPPTTPPPTTPPPSGTKADNPYAGVQGYVNPEWKAKADAEPGGNRVSSNPTAVWLDRIAAIAGTTGSSSNGSMGLRDHLDAALAQGAGYVQFVIYNLPGRDCSALASNGELGVNDLPKYKSDYIDPIAAIQADPKYAGLRIINIIEIDSLPNLVTNTNVATCATMKANGGYVQGVGYALNKLGAIGNVYNYVDAAHHGWIGWDTNFGPTADIMYQAAVASGNVKNVHGFITNTANYSALTEPHFNINTNVNGTSVRQSKWVDWNYYVDELSFAQAFRTKLVSTGFDAGIGMLIDTSRNGWGGSGRPAAASTSTDVNTFVNSSRIDRRIHAGNWCNQDGAGLGERPKAAPAAGIDAYVWVKPPGESDGSSTLIPNNEGKGFDRMCDPTYTGNERNGNSATGALAGAPISGAWFSAQFRQLMANAYPALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W897</accession>
    <name>U5W897_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative threonine dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_35565</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561705.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35565"/>
    <dbReference type="EnsemblBacteria" id="AGZ45369">
        <property type="protein sequence ID" value="AGZ45369"/>
        <property type="gene ID" value="AFR_35565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7198"/>
    <dbReference type="eggNOG" id="COG1171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_2_11"/>
    <dbReference type="OrthoDB" id="1876944at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="19"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="315" mass="32650" checksum="3BF8D50D67F9E38F" modified="2014-01-22" version="1">MDLSFADVAAARDIVYRHLPPTPMWSYPVLDAAVGATVHVKHENVQPVGAFKVRGGLTLLDAMSAEDRARGTVSYSTGNHAQSLAYASAAYGAPCTVVMPAAANPAKANAVAALGAEVILHGPDLEAAQHHAEALARETGARLVSPGDTPELLAGVGTLYQEILESQPDLDAILVPIGSGTGASAATVVARELAPKCHVIGVQSEASRAAYDSWRTGELVKRPNRTRVDGLATGRGFDLPQRLMRNGLSDFLLVTDDQITQAQRLLATKAHTLAEGAGAAALAGVLSRPDLFTGKRIAVVCTGGNANDAELAALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8S1</accession>
    <name>U5W8S1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Elongation factor P</fullName>
            <shortName evidence="7">EF-P</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">efp</name>
        <name evidence="11" type="ORF">AFR_30650</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7 8">Belongs to the elongation factor P family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560725.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30650"/>
    <dbReference type="EnsemblBacteria" id="AGZ44390">
        <property type="protein sequence ID" value="AGZ44390"/>
        <property type="gene ID" value="AFR_30650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6206"/>
    <dbReference type="eggNOG" id="COG0231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074944_0_1_11"/>
    <dbReference type="OrthoDB" id="1260763at2"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04470">
        <property type="entry name" value="S1_EF-P_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05794">
        <property type="entry name" value="S1_EF-P_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00141">
        <property type="entry name" value="EF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015365">
        <property type="entry name" value="Elong-fact-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020599">
        <property type="entry name" value="Transl_elong_fac_P/YeiP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001059">
        <property type="entry name" value="Transl_elong_P/YeiP_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013852">
        <property type="entry name" value="Transl_elong_P/YeiP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011768">
        <property type="entry name" value="Transl_elongation_fac_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30053">
        <property type="entry name" value="PTHR30053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01132">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09285">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005901">
        <property type="entry name" value="EF-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01185">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00841">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00038">
        <property type="entry name" value="efp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01275">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EFP" evidence="10">
        <location>
            <begin position="67"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Elong-fact-P_C" evidence="9">
        <location>
            <begin position="129"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ44390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="20091" checksum="458BF825BF9DD9BD" modified="2014-01-22" version="1">MASTNDLKNGLVLNLEGALWAVVEFQHVKPGKGGAFVRTTLKNVLSGKVVDKTFNAGTRVETATVDKRTMQYLYQDGEDFVFMDLDTFEQITVPGPTVGENANYLLPEAEATVATHETVPLYIELPTSVFLEVTYTEPGLQGDRSTGGTKPATVETGATVNVPLFITTGEKIKVDTRDGRYLGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9U5</accession>
    <name>U5W9U5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Robl_LC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561088.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32460"/>
    <dbReference type="EnsemblBacteria" id="AGZ44750">
        <property type="protein sequence ID" value="AGZ44750"/>
        <property type="gene ID" value="AFR_32460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6569"/>
    <dbReference type="eggNOG" id="COG2018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118613_0_1_11"/>
    <dbReference type="OrthoDB" id="1736176at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="1">
        <location>
            <begin position="14"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="146" mass="15386" checksum="79E6F0366980F63A" modified="2014-01-22" version="1">MHSATTDQQYKAIRGELSALRHQVTGVQGCVIAGVDGLLILHDTMPGTEPHDLAALAAGAHGISRTCGSALNQGGFHECTIRNHQGYLAVYAVGDLALLAVLGDSGLNIARLHLEARQVTGRLAKLLELKPLQDSHPLDGRTEPIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9X6</accession>
    <name>U5W9X6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative glutamate--cysteine ligase 2</fullName>
            <ecNumber evidence="2">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Gamma-glutamylcysteine synthetase 2</fullName>
            <shortName evidence="2">GCS 2</shortName>
            <shortName evidence="2">Gamma-GCS 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37840"/>
    <dbReference type="EnsemblBacteria" id="AGZ45822">
        <property type="protein sequence ID" value="AGZ45822"/>
        <property type="gene ID" value="AFR_37840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7652"/>
    <dbReference type="eggNOG" id="COG2170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044848_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042398">
        <property type="term" value="P:cellular modified amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01609">
        <property type="entry name" value="Glu_cys_ligase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011793">
        <property type="entry name" value="YbdK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02050">
        <property type="entry name" value="gshA_cyan_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="372" mass="40907" checksum="D5243D233CA92ED7" modified="2014-01-22" version="1">MAGEAAAQGLLTLGVEEEYLLVDAVEPRAVEAVEEVFDQLSDDIRDSVQHELMRSQIETASPPQLELGDLFDSLRRLRTGLAASAERAGVRLIAVGAAPAPGQASRIVDSPRYHRMRERFGDLSPGAGLNGMHVHVSVPDPETGVQILNHLRPWLPILQAATANSPLFAGRDTGYASWRSMLWERWPTVGPTPYLESHEQYETMVSDLVASGAMLDEGMLYWYARLSANYPTVEIRMGDVCPTLDITILLAALTRGLVSTLLREVEDGKPAPNVPHPLVMAAHWRAAHDGLEGLNIDMATREPKPAWKLMRQLFDYVTPELDRHGDLELVTVLMDRLRSSGTGAARQRALLSKQGSVEDVVDWLAAATRGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9X7</accession>
    <name>U5W9X7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_38450</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38450"/>
    <dbReference type="EnsemblBacteria" id="AGZ45944">
        <property type="protein sequence ID" value="AGZ45944"/>
        <property type="gene ID" value="AFR_38450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7778"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="115"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="33535" checksum="6213076D3AC85F7A" modified="2014-01-22" version="1">MPIKYPSARAGLEGVSGALPAPGAKDEITDTAAADRDRLTGLDALELGPKKSKAAIGGRIWRSAWPKLLAIGIVVLIWQIVVWSQWKPVYVLPGPATVFGDLGTLITTGDFWDGVRLTMIRALTGFALAVLIGTVVGAAVSRFAPLRAAIGSLITGLQTMPSIMWFPLAILLFQISESAIMFVVIIGAAPSVANGLISGIDYVPRTWLRVGQVLGMKGFAKYRYLILPASLPSFISGLKQGWAFSWRSLMAGELLVIVPGALSIGVRMQQARDLSESSLVISFIIVVLVIGILIDVLFNAADNALRKRWGLTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Y3</accession>
    <name>U5W9Y3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cellulose synthesis regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562347.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38795"/>
    <dbReference type="EnsemblBacteria" id="AGZ46013">
        <property type="protein sequence ID" value="AGZ46013"/>
        <property type="gene ID" value="AFR_38795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7850"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_528576_0_0_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="386"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="515" mass="56893" checksum="5C78A6871F957144" modified="2014-01-22" version="1">MESNRLPVPVSPYGPYAALALHVHDLTIRGHHTETLNAADEAEAVTLLLGDHRTRRVSRLGRMYALSALGRLDEALTIAEELVSRPAGPRATDAKIMADTAEVLMHLGRIDDGLHYVARAMNLLDVAPNKGIRYFSAMASLCNAARSADLFELADEVMHKALNSFDSPDDLYRTGAELEHAEMWLEWALRLEQVGRIEEASALVDKAVALLEFWVDKGLDSPIGVSALAVGYAKTGRHDDVFRLVGELLLKMRDSGQQHEARLLHLAHGLSLRATGDLAAARREFRAAEELSVLASQRLLFRYELAVTAVLEFPGEAMQTVLESLRSHVGALWQLRLDRRTMLRQAYRRVELEAARTTADLAATSDALTGLGNRRMFDSRMDSVEGGGSLLLIDVDRFKDINDRFSHGVGDRVLGEIASILRSHCRHDEVAIRFGGDEFAMFLSTGEPEAAQIAERIRRVILARDWNTIALGLRVTLSMGLAACQDGEKGRDLYDRADARLYTAKRSGRNQLAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAA8</accession>
    <name>U5WAA8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562378.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38950"/>
    <dbReference type="EnsemblBacteria" id="AGZ46044">
        <property type="protein sequence ID" value="AGZ46044"/>
        <property type="gene ID" value="AFR_38950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7883"/>
    <dbReference type="eggNOG" id="ENOG5032UXI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1249538_0_0_11"/>
    <dbReference type="OrthoDB" id="1701022at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="232" mass="25836" checksum="8564616EF7323E36" modified="2014-01-22" version="1">MSTLLRRNIGFLICLAAAAVCAGIIWFALPHDREVKSLGIFLFKLVPFIFAAEALARLDVAFFQRIKAVRVLVPVAFMVYFLYFVPKIFFYAEDHPNLYYHILTLTPMIILSFVLAFRLGGGDAGTSRRLSYALLLIMLSGAEDLAYLTVNTFAPPYDKIPAVWDWASHMTVFLGHPASKYEAFAFIAVHLVLAALALFAPAAWFAKLNPWRRNTGTATAEAGTVEREPAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAD7</accession>
    <name>U5WAD7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39100"/>
    <dbReference type="EnsemblBacteria" id="AGZ46074">
        <property type="protein sequence ID" value="AGZ46074"/>
        <property type="gene ID" value="AFR_39100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7913"/>
    <dbReference type="HOGENOM" id="CLU_145448_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039470">
        <property type="entry name" value="Nuc_deoxyri_tr2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15891">
        <property type="entry name" value="Nuc_deoxyri_tr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="14080" checksum="B6735612961D6C56" modified="2014-01-22" version="1">MADWQAEAIGWLRPVWPAVYNPRRADFPMGDAGEGARQIRWEFEQLAVADAILFWFSFETTQPIVLYELGRWAASDKPLAVGADPRYERRFDVVEQLALARPGLTVHTDLPSTCAAANRFVGEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBA3</accession>
    <name>U5WBA3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative trypsin-like serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40065"/>
    <dbReference type="MEROPS" id="S01.494"/>
    <dbReference type="EnsemblBacteria" id="AGZ46267">
        <property type="protein sequence ID" value="AGZ46267"/>
        <property type="gene ID" value="AFR_40065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8108"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="292"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="392" mass="39117" checksum="8B18A79E17C7C3B8" modified="2014-01-22" version="1">MSDPWRDPYAPSAVVVPTAPVSTGPQLEPIADPDAPRRSMTPILLICLVTALLAGGLGGTLGFVFATRSGVGGGGTQLGGTPLAAPSAAQRAPESLAGVAKQVLPSVVTVRVTGAIGSGFVISSDGYVVTNDHVVESADKTLSVSFSDGSTASATVVGRDPESDLAVIKVAKDDMTAVQLGDSDSIAVGDPVLAFGSPLALVNTVTSGIVSALDRTIQAGDPGGQVRYYAAIQTDAAVNQGNSGGPLVDAAGRVVGVNSVIRSVGGSDTEAGNIGLAFAIPINQAKRIAKDIIDFGKARRTVIGAEVATGGSTGSTGSVGARLRSVEPAGPAATAGLKSGDVITKINGHVLEDGTDLIALVRKYAPASVVAVEYRRGTKIQSASVTLAADAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBJ5</accession>
    <name>U5WBJ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562856.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41350"/>
    <dbReference type="EnsemblBacteria" id="AGZ46524">
        <property type="protein sequence ID" value="AGZ46524"/>
        <property type="gene ID" value="AFR_41350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8368"/>
    <dbReference type="eggNOG" id="ENOG502Z86B">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082565_1_1_11"/>
    <dbReference type="OrthoDB" id="1352255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025633">
        <property type="entry name" value="DUF4291"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38567">
        <property type="entry name" value="PTHR38567"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14124">
        <property type="entry name" value="DUF4291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="191" mass="21826" checksum="7A997FBFB49DB348" modified="2014-01-22" version="1">MEVPQRQIRAAFTDKTITVYQAYPVQIAEPAVRAGHFVPPFKLERMTWIKPSFLWMMYRCGWGAKPGQERVLAIDISRTGFEWALAHACISHHSPGSGESQADWSRRLRKSPVRVQWDPERNIQLQPLPYRSLQLGLGREAVGRYVDEWTVAIRDVTDLAHQIQATRDPALLPAEQPYPLTPELATTLEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBM7</accession>
    <name>U5WBM7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45405">
        <property type="protein sequence ID" value="AGZ45405"/>
        <property type="gene ID" value="AFR_35745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7235"/>
    <dbReference type="eggNOG" id="ENOG50331MA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929482_0_0_11"/>
    <dbReference type="OrthoDB" id="1776231at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="33714" checksum="699AF3AB0AF0D97A" modified="2014-01-22" version="1">MGYSLDAWLALGPDPALAVLRSFPVWEPDEWQLESSCEAGALLDLDTRELLFYLDLDYERRAALLDGYGATWPGWQIRWAYNGISDVTDALGLPSTVLEHEPWDSTDLYRWGRAAADEEPIVRYLVTVGEVAYALDPYAGQPWQVGPALLEQLTDGHRVTGWPEVPRGGLHLDPGSRTAGIWCIEPVLGVAERFAGRWPGWTLQWWEDRYDEQERRAGGTFVPPELGAGRFELAERVQDHWVYATAEMSGHWPEFRNDYGTRDAGLTVDELQRGCDLLSGPEGDRVDVGAEAAKLRRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC33</accession>
    <name>U5WC33_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-like N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563030.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42225"/>
    <dbReference type="EnsemblBacteria" id="AGZ46699">
        <property type="protein sequence ID" value="AGZ46699"/>
        <property type="gene ID" value="AFR_42225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8547"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106319_1_0_11"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="23424" checksum="B03CA5F5266A1934" modified="2014-01-22" version="1">MITEHWPLFGLRLFTPRLELRVPSLDDLGQLAGVAATGVHDPDRQPFVVPWTDGSPAERARGTLQWHWGQWSAWSPAKWSIGLVTVLDGRVIGTQEMDARNFGVLREVGTGSWLGREFHGRGYGTEMRAAVLDLAFTGLGAEQATSEAFDDNAASYAVSRKLGYADDGIARHVVRGRAATGRRLRLDRAGWEAARTVEVRIEGLEPCLPMFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC98</accession>
    <name>U5WC98_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36820</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561956.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36820"/>
    <dbReference type="EnsemblBacteria" id="AGZ45620">
        <property type="protein sequence ID" value="AGZ45620"/>
        <property type="gene ID" value="AFR_36820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7449"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_15_11_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="203" mass="22350" checksum="4E6BF7E2E7025074" modified="2014-01-22" version="1">MTERLTRAQQQERTRERLLNAAETLIGDRGIHQTSLDQIAAAAGLTKGAIYANFGSKKELIAAILDRKLTSDDGEPHEPLSSLDRWASSLGDSYSVNVERPEIRRFALAMIELWLYGMREDAGRAPVAEWLRTIRALNSKKAESLATGELPMPVDQLSALMTALDIGVAMQHYLDPEAVPPEVYRAGLEAVMRRPEASTPPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCF5</accession>
    <name>U5WCF5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563169.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42925"/>
    <dbReference type="EnsemblBacteria" id="AGZ46839">
        <property type="protein sequence ID" value="AGZ46839"/>
        <property type="gene ID" value="AFR_42925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8684"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_836295_0_0_11"/>
    <dbReference type="OrthoDB" id="1941916at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="32389" checksum="CE159DEE400FFAC2" modified="2014-01-22" version="1">MTGAEFSGVDVDLLADYVGGALEGTPDEAVVAALIADDPAWREAHELLSGGVVTVTSQLAEFGSAPEPMPADVIARLDAALLAVDAPADVIPIDRAFRDATAPRKETGTGDAGAAPATRLVAVPSEAPRKRARRLRWAAPIGIAAGVIAFIGFGAQGFTGGSDDTQTSSAGSAADEAAPRAAAGKDLAVPGVVAVLEATGTDYTLETLGQAGARAMAAPSLPEDAGSVRKSEPTAGVMSDTAAKDALSRLRVNEALLACINAIADESGLAPITAQSADFARYRGAPAVIVNFTSTGGTWVWAVGPECGTRGVGADKLGSVQVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCH4</accession>
    <name>U5WCH4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_37230</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45700">
        <property type="protein sequence ID" value="AGZ45700"/>
        <property type="gene ID" value="AFR_37230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7532"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="386" mass="40558" checksum="CFBC1FB24EFAF92B" modified="2014-01-22" version="1">MEVPVTADDSPAEAEAVHPPADDPEPSADAPEEPKGPGQFGRPGPPLSRRNPFLIGLLGGLGLITAYGIFLGLRNAASILVLIFIALFLAIGLNPAVTRLRAWGLPRGPAVAVLALGIVLLLCGGVFALVPPLVTQTGQLIQNVPSFIESLQRNETVNDLVQRYDVLSKVQSALNAGTVTNALGGVVGGARLLFGTIFNVLTVIVLVIYFMAAFDRIKEGGYALVPRSRRDRVRLLTDEILSKVGAYMVGAIAIAVLAGLSTWVFAMIVGLAYPFALAVVVAVCDLIPQIGATLGAVIVSLVGFASSLTTGIVCLVFFLIYQQVENYLIYPNVMRRSVKVSDVAAVTAALLGVGLFGVIGALIAIPMVAAIQLIVREVVNPSMEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCN2</accession>
    <name>U5WCN2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamate dehydrogenase</fullName>
            <ecNumber evidence="2">1.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_37135</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamate + NAD(+) = 2-oxoglutarate + H(+) + NADH + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.2"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.4.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562017.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37135"/>
    <dbReference type="EnsemblBacteria" id="AGZ45681">
        <property type="protein sequence ID" value="AGZ45681"/>
        <property type="gene ID" value="AFR_37135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7513"/>
    <dbReference type="eggNOG" id="COG2902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003404_1_1_11"/>
    <dbReference type="OrthoDB" id="79339at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019551">
        <property type="term" value="P:glutamate catabolic process to 2-oxoglutarate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028971">
        <property type="entry name" value="NAD-GDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007780">
        <property type="entry name" value="NAD_Glu_DH_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43403">
        <property type="entry name" value="PTHR43403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05088">
        <property type="entry name" value="Bac_GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036761">
        <property type="entry name" value="GDH_Mll4104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1654" mass="182966" checksum="5615DF13EE73130A" modified="2014-01-22" version="1">MAVADEAAIESDPELDFGPGPGLALTGRLGTSDDELDEPLPNAERLVAQAVALAGEDHTTASLVGRFWRFAPDEELVGYTAAEMHAAAVAHRELAATRLPGQLRLDISAPDQLEPHTVISIVTDDMPFLVDSVTALLTAHQLDVHLLVHPLIVVRRRETGELGQVEADVEPDDAIDGDLVESWIRVEIDPVRKAEAREQLSQELCRVLTDVRDAVDDWPRMRQRAVVIADELAAARGSDRKLPVPDKDVTDSIELLKWLAHDHFTFLGYREYHLDGDVLNAVPGSGLGILRGDNSTPRKLSSMAPEAHQRALEKRLLVITKANSRATVHRSAYLDYIGVKVFNESGEVVGERRFLGLFSSSAYRTSVRELPVVRRKVMEVLDRSGLSPRGHSGKDLLQILETYPRDELFQIKTDDLYEAVVGVLRMAGRRQLRLFLRRDGYGRFISCLIYLPRDRFTTANRQSMQEILLRELNGIGVDYTTRVTERMLARVHFIVRTDPADPPGAVDPNTLAELLADATRMWDDDFSLVLERKLGEEPAKDLFHRYSIGFPDSYKDGHTPYEAVQDIAKLELLEETGQLEMHLYRKRHVNREGCAEPDDADVRFKVFRYGEPMMLSAVLPVLHSLGAQVVDERPYEVRRPDGTVYLYDFGLLPPASHRELSEVRPQVENAFAAAWRGEAEVDGFNELVLRAGLTWRQVVVLRAYAKYQRQTGNVFSQRYVESTFIAYPEIAGLLVQLFEVRFSPRLELVEEERERRGADLAEQITTLLDDVDSLDQDRILRSYLTLIQATLRTSFFQRGADSRPKSYVAFKLDPQAIPDLPAPRPKYEIFVYSPRFEGVHLRFGAVARGGLRWSDRREDFRTEVLGLVKAQMVKNAVIVPVGAKGGFVLKQKPGDRDEAVACYRLFIGALLDVTDNIHSGKIVPPVDVVRHDSDDPYLVVAADKGTATFSDIANEISVSKDFWLGDAFASGGSAGYDHKKMGITARGAWESVKKHFRDLGTDTQSQDFTVVGVGDMSGDVFGNGMLLSEHIRLVAAFDHRHIFLDPEPDAATSYAERRRLFDLPRSSWADYDAALISAGGGVYPRTAKSIPVSPQVRAALGLGDATAVSPADLMRAILTAPVDLLWNGGIGTYVKATAETHADVGDKTNDPIRVNGAQVRAKVVGEGGNLGLTQRGRIEYARTGGPQDETGTGHGGRIFTDFIDNTAGVDCSDHEVNIKILLGGAVTDGEMTVPARDELLAAMTDEVAALVLRDNYEQATALGNARSQAHSLLPVHRRMLITLEQSGQLNRELEALPTDKELAARYDAGEGLTAPEFAVLLAYVKISLERQVLADVLVDEEWTNGVLARYFPTPLRERFGARMAGHRLRREIISTALVNEVVNRGGTSFVFRAMEESGASAADVIRAYVVIRDVYGLPDLWAAAEKLDNNVPTPAQTLVYLETRRLLDRAVRWLVSNRRSPIDVAGEIARLRPGVDALLQQLPTVVIGKERSSLENHIEMLAERGIPEGLAESVSRVVYSFGLLDILETAAATDHAPTEVAKVYFVISERFQIDVLLSHISRLPRGDRWQTLARMALRYDLYAALAGLTAEVLQSTSAEATPEDRVSEWEQVNAASMARAGNAMGNVEESPADLAALSVLLRQIRTLVKTSAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCT0</accession>
    <name>U5WCT0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37780"/>
    <dbReference type="EnsemblBacteria" id="AGZ45810">
        <property type="protein sequence ID" value="AGZ45810"/>
        <property type="gene ID" value="AFR_37780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7640"/>
    <dbReference type="eggNOG" id="ENOG502ZREQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891705_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="128" mass="12804" checksum="5F9859554BD0381D" modified="2014-01-22" version="1">MALSSTFLLLAAGGAAACDNGNDYQDEGFYCADENGVIVDEDYCDDDNGGGGGGGGFFIWHSSGYRSGYPVGSRLPSGGSKFAYNDRAARSSFGLPSSGRISNGTVKTSVVGKGGSGTSGKSSSRSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCT1</accession>
    <name>U5WCT1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562071.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37410"/>
    <dbReference type="EnsemblBacteria" id="AGZ45736">
        <property type="protein sequence ID" value="AGZ45736"/>
        <property type="gene ID" value="AFR_37410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7567"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_540671_0_0_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="381"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="501" mass="53123" checksum="61B388F03A66D88A" modified="2014-01-22" version="1">MTWLDQLPDSVDELRDAGNFQQVGRSAEALAILDKVLATTADPLTRAYALVQRFGALINLGRAAEFATAMTLASNAVRETSDPYLRGQMHAFAALGAHLQSALDRGVTHLVQASRALAAVPDGDSETAWGWHDLAMAYSYLGFHGHALTAIEQARQVGAAAGMASEVFAAPGIRLRNAVSLDHQGDTDGCLRVLRDIDAELTRYVASGADVRLRPSSRPVYGYALARRAALGESTEADVAGLLTGGGDSVRSRDLRHLGTVCLDIAADRPADALRRLDAVPVSPEILGPAEPARLRSICYASAGNHEAAHTADRHAFRLAAQRIDRLRDGYLDGVAARLDAQETQRDVGRYGDETLTDPLTGLPNRRQLERYVAAMLARGERAAIGVCDLIGFTAVNARHGRHCGDLVLQRIAGVLNRVMRRGDFVARFAGDEFVVVLPGAGPTQAAEVSRRISAAAAGENWQVLVPGTPIGLAAGWSEVGTNGRSLSAALAAAASNRTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCU8</accession>
    <name>U5WCU8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_37880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562165.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37880"/>
    <dbReference type="EnsemblBacteria" id="AGZ45830">
        <property type="protein sequence ID" value="AGZ45830"/>
        <property type="gene ID" value="AFR_37880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7660"/>
    <dbReference type="eggNOG" id="COG1716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_721225_0_0_11"/>
    <dbReference type="OrthoDB" id="623871at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SSF49879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="3">
        <location>
            <begin position="263"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="40993" checksum="5B0CCD6F64B56D18" modified="2014-01-22" version="1">MRFEVSKVLDAIEARLTTDPALARAVVDLSEIVRYADLDGGRPASMLRLGLVIDALGRHVSEENVPVYAVVPRGLLSDADLTSNERMVVRRWADDGVVEVVPQLDDRVLEAAELLGLPVLTRTRAEQFRDRRPWVEEPGRLLAAVPGAGGPVLVARIGRGEIPAVGEPSPMGQKLLARVWSCPEPNCNAFGSGGDPDSPFSDMRKFTAPVAQPPPSLRSGAPTCPRHGSRLKDAGPRPEVEVLSVRIDGVVRQRFVVSTDKPVVVGRAPEGGGVMLGQWLTDDARKWISRGHVRLALHNGELTAQDVSTNGTGIRPGGSTDDDDRVTLNRDETRVLAGNDVVELYGGVNVGRAKLWATGGVTQPSSVMAEAPTMAIRKFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDY7</accession>
    <name>U5WDY7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative diguanylate cyclase with GAF sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_39905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39905"/>
    <dbReference type="EnsemblBacteria" id="AGZ46235">
        <property type="protein sequence ID" value="AGZ46235"/>
        <property type="gene ID" value="AFR_39905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8074"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021525_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="3">
        <location>
            <begin position="228"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="503"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="691" mass="72236" checksum="FB1BC2BD7E174CC4" modified="2014-01-22" version="1">MAVVLGPVLLGSFFVGMTVATVSQDRTVERLDHAATTVRTAVGAMCRQLQAAADAVAVLPEEARRPAADQLVTRGLASEIRIGPEGSADQRDCAAPAAGGSVTAIVARARQGEITVSAAQRLDAGFVNRLAASSGAEVTLLAGDSPGAGPALSSETDRVSRRVAAALPGSMTRRGHHHVRGIPAEPGQPLPLAVSVPSSDPMSLYAFLLGGVVIVACGAIVVARRLARSTTRPLEELVWAVGKVADGELGTRVPVGRDDELGKLAGTFNRMTRELQSYVQALTASRDQLRGHLAILGDTLSSTHDLHRILQVILQTALSATGARAGIVLLLDPVDGRLVARCAEGLTGRWDVPEADLPALRMTPGAGVLGAVAATGEPRRGSFGPGAGALDEPACRTYVAVPICAPATVGDPLVPGDALVSGQPATLGVLALYDRLGGDEFDDADLLTLRTFAGQAGVAVHNVRVHEEAQRLSLTDPLTGLWNYRYLRESLRREVERASRFGRMLTVLVLDLDHFKAVNDTYGHAAGDTVLGEFARRIRIGLREVDVAFRQGGEEFVVLLPETDAYGGVIVAERLGAAVRDIPVPIDPRRQDSVTGLPLDRVSISVSIGIAVYPEHGTTAQQVLDAADDALYAAKNAGRDTYRLAESVPLPATGVTETAVSAGVPEGQFDQTAGPDVSSGGPQPPRQGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEQ7</accession>
    <name>U5WEQ7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chaperonin GroEL</fullName>
            <ecNumber evidence="9">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Chaperonin-60</fullName>
            <shortName evidence="9">Cpn60</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">groEL</name>
        <name evidence="9" type="synonym">groL</name>
        <name evidence="12" type="ORF">AFR_40885</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 11">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Capsule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9 10">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562763.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40885"/>
    <dbReference type="EnsemblBacteria" id="AGZ46431">
        <property type="protein sequence ID" value="AGZ46431"/>
        <property type="gene ID" value="AFR_40885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8278"/>
    <dbReference type="eggNOG" id="COG0459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016503_3_0_11"/>
    <dbReference type="OrthoDB" id="265347at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042603">
        <property type="term" value="C:capsule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="29"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="477"/>
            <end position="479"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ46431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="540" mass="56571" checksum="ADE8667502DD6065" modified="2014-01-22" version="1">MAKIIAFDEEARRGLESGMNQLADAVKVTLGPKGRNVVLEKKWGAPTITNDGVSIAKEIELEGTFEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREGLRNVAAGANPMALKRGIEAAVASVSEELTKIAKDVETKEQIASTASISAGDSTVGEIIAEAMDKVGKEGVITVEESNTFGLELELTEGMRFDKGYISAYFMTDAERMEAVFDDPYILIANSKISAVKDLLPVLEKVMQGGKPLIIIAEDVEGEALATLVVNKVRGTFKSVAVKAPGFGDRRKAMLDDIAILTGATVVSEEVGLKLDAADLSLLGQARKVVITKDETTIVDGAGNAEQIQGRVNQIRAEIEKSDSDYDREKLQERLAKLAGGVAVIKVGAATEVELKERKHRIEDAVRNAKAAVEEGIVPGGGVALVQAGKTAFDKLDLVGDEATGANIVRVALDAPLRQIAVNAGLEGGVVSEKVRNLELGHGLNAATGEYVDLLAAGIIDPAKVTRSALQNAASIAALFLTTEAVVADKPEKNPAPAGGPGGGDMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF14</accession>
    <name>U5WF14_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="3">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Alpha-IPM synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">leuA</name>
        <name evidence="5" type="ORF">AFR_41755</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41755"/>
    <dbReference type="EnsemblBacteria" id="AGZ46605">
        <property type="protein sequence ID" value="AGZ46605"/>
        <property type="gene ID" value="AFR_41755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8450"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004588_3_0_11"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07942">
        <property type="entry name" value="DRE_TIM_LeuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00572">
        <property type="entry name" value="LeuA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005668">
        <property type="entry name" value="IPM_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039371">
        <property type="entry name" value="LeuA_N_DRE-TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00970">
        <property type="entry name" value="leuA_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="4">
        <location>
            <begin position="31"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="562" mass="62378" checksum="50A3507B8B34CB5B" modified="2014-01-22" version="1">MPFERYQPYEKQFAVDLPDRQWPAKRIEAAPRWCAVDLRDGNQALIDPMSPERKRRMFNLLVAMGYKEIEVGFPAASQTDFDFVRQLIEQDLIPDDVTIQVLVQCREHLIDRTFESIRGAKRAIVHFYNSTSTLQRRVVFGLDKDGITDIATSGARLCQKYAEIHTPDTEIFYEYSPESYTGTELEYALEICSAVIDVIDPTPDRPLIINLPATVEMATPNVYADSIEWMHRHLPRRESIILSLHPHNDRGTAVAAAELGVLAGADRVEGCLFGNGERTGNVDLVTLGLNIFSQGIDPMIDFSDIDEVKRAVEYCNQLPVHERHPYVGDLVYTAFSGSHQDAIKKGLTALQADAADAGVEVDKFTWGVPYLPIDPKDVGRSYEAVIRVNSQSGKGGVAYIMKEEHNLDLPRRLQIEFSGVVQHFTDTDGGEVGPQAMWDIFAGEYLVDHQQFGIVDFERYTTATVDGKVEIDAEIRHRGTRRPIVGVGNGPIDAYVQALAPLAIQVRVLDYAEHALSSGGDAQAAAYVECEVNGRSVWGVGLDANIVTASIKAVTSAVNRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFI7</accession>
    <name>U5WFI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42135"/>
    <dbReference type="EnsemblBacteria" id="AGZ46681">
        <property type="protein sequence ID" value="AGZ46681"/>
        <property type="gene ID" value="AFR_42135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8526"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135672_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="15868" checksum="D68411DA9BEAA90D" modified="2014-01-22" version="1">MVDDAFVVPEVFVGDGFRLELLGPQHNEADHAAWMSSIEHIRSTPGFGRGWPPAAGMTPAQNLADLESHAERSARRVDFAYSVIEDVTGDVIGCVYLKPAVAQGEVDVSSWVSAARAELDGPLTHVVAAWLREAWPFRAVHYTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFN2</accession>
    <name>U5WFN2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein jag</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_42980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563180.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42980"/>
    <dbReference type="EnsemblBacteria" id="AGZ46850">
        <property type="protein sequence ID" value="AGZ46850"/>
        <property type="gene ID" value="AFR_42980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8697"/>
    <dbReference type="eggNOG" id="COG1847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042512_3_0_11"/>
    <dbReference type="OrthoDB" id="1304273at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02414">
        <property type="entry name" value="jag_KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02644">
        <property type="entry name" value="R3H_jag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038008">
        <property type="entry name" value="Jag_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039247">
        <property type="entry name" value="KhpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034079">
        <property type="entry name" value="R3H_KhpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35800">
        <property type="entry name" value="PTHR35800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="SSF82708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="4">
        <location>
            <begin position="125"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="191" mass="20562" checksum="9A1DC9A203A4078B" modified="2014-01-22" version="1">MTDTSTPSAEQSTEETASATAAESAGTEETKKSETVTTDSDLFRQSEIAADYVEGLLDILDYDGDIDELVSAGRPMVEVVGGRLQPLVGQRGATLEALQELTRLAIFRATGSPSRLLLDIGGYRATRRKELTAVARNAVEKVKEHGDAVRLEPMSAFERKCVHDVVNAISGVQSESEGVEPSRRIVVRPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNE5</accession>
    <name>U5VNE5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">HTH-type transcriptional regulator betI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_00165</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357266.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00165"/>
    <dbReference type="EnsemblBacteria" id="AGZ38322">
        <property type="protein sequence ID" value="AGZ38322"/>
        <property type="gene ID" value="AFR_00165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.34"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_22_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041490">
        <property type="entry name" value="KstR2_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17932">
        <property type="entry name" value="TetR_C_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="22113" checksum="F8C8C2FC35E60F8C" modified="2014-01-22" version="1">MTVDQQQRPGPPAPRRRSRRDEILEIAVGLFAARGYHGVSMDDIGSAAGVTGPALYHHFAGKEAMLVAALIPVSEGLLHGGKERVERHPDDAQAALTDLIEFHVEFALANPAVIALHLHELDRLPEEPKRQIRRLQRLYVEEWVGVLAMLRPELEAGEARVLAHSAFGLMNSTPFLGGEVDRRRRAVLLREATVHALIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNF7</accession>
    <name>U5VNF7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357446.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01070"/>
    <dbReference type="EnsemblBacteria" id="AGZ38503">
        <property type="protein sequence ID" value="AGZ38503"/>
        <property type="gene ID" value="AFR_01070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.213"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_11"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="297" mass="32342" checksum="48B80AA74365D01F" modified="2014-01-22" version="1">MAEVRMSSDALAAFAVFAEHLNLTRAAEVLRISQPSLHSKLATLARHLGRPLYQRTGNRLELTPDGERVARFARDHEERLTAFLDELRGTPPTRPLVIASGHTAYLYILGDTIRTMLGERPGSLRPLHTNRHQMQAAVRTGRAHLGVFSLHVLPDDLVTLPIATYPQVLLMPDDHRLARKRSLGLSDLAGMDLIVPPPARPHRINLEQALTNAGVTWTVAVEAEGLPMTLDFAALGVGLAVVAGCVRPAPGLTTRRITDLPQVTFHAVHRPGALDDPRTAELLGRIRAGVPASARRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNP3</accession>
    <name>U5VNP3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38414">
        <property type="protein sequence ID" value="AGZ38414"/>
        <property type="gene ID" value="AFR_00625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00625"/>
    <dbReference type="HOGENOM" id="CLU_1912566_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="132" mass="13826" checksum="AEDB966F2C450452" modified="2014-01-22" version="1">MAPTLLTRGRYEAVRPGRTDSLVVPEVARSTPWPMRTWDLLVVVGPPSTRVVITTCPASPGCAPAVARTWKTPIGTTKAPASIMVVQVRTGLFPVQGEALGGLDAPCVTMMFCSMSSGTIVAKAATAEKSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPC3</accession>
    <name>U5VPC3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ESAT-6-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WXG100 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357587.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01775"/>
    <dbReference type="EnsemblBacteria" id="AGZ38644">
        <property type="protein sequence ID" value="AGZ38644"/>
        <property type="gene ID" value="AFR_01775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.358"/>
    <dbReference type="eggNOG" id="COG4842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151185_3_0_11"/>
    <dbReference type="OrthoDB" id="1930308at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036689">
        <property type="entry name" value="ESAT-6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010310">
        <property type="entry name" value="T7SS_ESAT-6-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06013">
        <property type="entry name" value="WXG100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140453">
        <property type="entry name" value="SSF140453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03930">
        <property type="entry name" value="WXG100_ESAT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="10599" checksum="44BC226124B1CFBC" modified="2014-01-22" version="1">MNDGLLKVNFSALAQAGADIQKALNELESQLSQLEADARPLVATWEGKAQTAYALRQQQWTQASTDLKGILRDIKVAVDQSATDYATTEGNAEKRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ28</accession>
    <name>U5VQ28_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03125"/>
    <dbReference type="EnsemblBacteria" id="AGZ38914">
        <property type="protein sequence ID" value="AGZ38914"/>
        <property type="gene ID" value="AFR_03125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.629"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="29678" checksum="0376D48862C8B0CC" modified="2014-01-22" version="1">MPGTPSGPNEEGNTMTTPTGRDFAVVTGASSGIGYELARQFAENGFDLLLAAEDEGITQAAADLRRDGVNDVQAVQVDLATYDGVEELYAAIRAAGRPVDAIAINAGRGIGGDFTRQTDLSDELNVIDLNVRSTVHLAKRVLPDLVSRGAGRVLFTSSIASTMPGTYQAVYNASKSFVQSFAEALRNELKDTGVTVTSLMPGPTDTNFFDRAEMEDTKVGASKKDDPAQVAEQGFKALMKGEEKVVAGSLKTKVQGAASKVMPDSVKAQMHRKMAEPGSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR45</accession>
    <name>U5VR45_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative cell envelope-related transcriptional attenuator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_04965</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358343.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04965"/>
    <dbReference type="EnsemblBacteria" id="AGZ39282">
        <property type="protein sequence ID" value="AGZ39282"/>
        <property type="gene ID" value="AFR_04965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1003"/>
    <dbReference type="eggNOG" id="COG1316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016455_0_2_11"/>
    <dbReference type="OrthoDB" id="491753at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665599" evidence="3">
        <location>
            <begin position="28"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="LytR_cpsA_psr" evidence="4">
        <location>
            <begin position="86"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="38574" checksum="ED2048CFA7FB2576" modified="2014-01-22" version="1">MRTNKTLWVVVGAVVVLLLAGAGIVVALRSGSDEPDPVAAPSAGPPSAPPASASPAPPPGADITGPLDVLILGVDTRVSIPDWQPHADAIMLLHVDADLKSGYLYSLPRDLRVDVPAFAKNGFDGGNHKLTEAMSYGARVRGTDKPNVEQGYELLARTIEKYTGIKKFDAGAILTFNGLSKLTDALGGVTLKIDQKVQSQHRRPDGTSRTLRSGGGGYVGPQATYLPGTRKLVGWQAIDYARQRYTAGGDYTRQRHQRQLVKALLTQAEDSGLATDQSKLQSVLTALGDTLVYSGERTPIEYAYALRDLTPSQLTLVGLPGASVSNGGGYIGEQLTTKGRGFLKALNAGTADAYLAKNRDLINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRR3</accession>
    <name>U5VRR3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00310"/>
    <dbReference type="EnsemblBacteria" id="AGZ38351">
        <property type="protein sequence ID" value="AGZ38351"/>
        <property type="gene ID" value="AFR_00310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.63"/>
    <dbReference type="eggNOG" id="COG3764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_405797_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05830">
        <property type="entry name" value="Sortase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042003">
        <property type="entry name" value="Sortase_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01076">
        <property type="entry name" value="sortase_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="677" mass="69060" checksum="6640DBEBB47EC32C" modified="2014-01-22" version="1">MVGSARPADPGRSASGFARPAGDVPAGDSFGRAAGGRLPANGAAAENDVYPTPTSSYARGDNGAQPQADGFRAPQGAAESFGRRNGAAQQASDGYPAGTFRQDNGHAEANNGFGPRSTPAERHHANDSESLATGTFSALGGDHSPTTNGFAPANGARSAPGDFARDAPGDRRSVADAFGARSSRRAATDAGAQSFDTPRSAAEIFGAQRSGNDAGPRGFGTPAPASDAGPQGFTPRSAGDGFGAPRSASGTSRPAGDAGPQGLAARSAMGGVDSGAQWSAGGGSQGFGARGLTSADDHDAGSDVAEPAGLLRRRGPGTEAAGFGQNGGQQAGSEHAAPQNGSDPSATAIIRTADAPTGLLPSVRPKAGSPSTPDVSGSVRTPLPPGPGDKGAASVAAAAAAVAAEPDAGAGGDEGDKPRRGEKVVKLRPEQTDEGYKSVYSELTRPTLGSRIRAGVRVSGELMITFGLIVLLFAGYEVFGNSAKVENEQDTLANQLDDQWNDPTVAPSAGPTKAAPAAPGKNLVGRLYIPKLGMEWVVVNGVRPQDIRYAPGHYPDTADPGKVGNFSVAGHRIRKIFWRLDELQPGDVIGVETRTNWYVYKVSRSEVVKPSAVEVVAPVPGKPNAKPTKAMLTLTTCNPKFNNYQRLIVHAELVDTVKRDASQPEAGKPAEMKKTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRW2</accession>
    <name>U5VRW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_00955</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00955"/>
    <dbReference type="EnsemblBacteria" id="AGZ38480">
        <property type="protein sequence ID" value="AGZ38480"/>
        <property type="gene ID" value="AFR_00955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.191"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="27"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="210"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="392" mass="41242" checksum="393DC113579DF568" modified="2014-01-22" version="1">MVRATGTAAHRVRMRIALVTESFLPDVNGVAHSVVRTAEHLVRRGHSPLVIAPQPPPGGRGVPDDLPYPVVRIPSLPMPGYPQIRLGLPTPALSAALRAHGTEVVHLASPFVLGAWGAAAARAAKVPSVAVYQTDVAAYARAYRVGMTEPAAWRWIRTVHNAAFRTLAPSTESVVALAAHGVRDVHLWRRGVDDVRFHPRHRNAGIRRALAPNGEVLVGFVGRLAVEKQVDLLAETSRLPGVRLVVVGHGPAAAALRRALPQAVFLGARHGAQLARIFASLDVFAHTGPYETFGQAVQEAMASGLPVVAPAAGGPLDLVADGRTGYLVPPFRADGFTAAIAALAADPAQRALLGAAGRVAIEGRTWAAVGDELIGHYTAALQPVRDRTLVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRX8</accession>
    <name>U5VRX8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidoglycan-binding domain 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359264.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07220"/>
    <dbReference type="EnsemblBacteria" id="AGZ39733">
        <property type="protein sequence ID" value="AGZ39733"/>
        <property type="gene ID" value="AFR_07220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1474"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057459_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665254" evidence="1">
        <location>
            <begin position="36"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="2">
        <location>
            <begin position="118"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="346" mass="35470" checksum="4A22FEFBCF247EE5" modified="2014-01-22" version="1">MSRRTVIAAAAVVLTAGAAAAVVGTLPDTPSEAEASTPPVVATVEKGTLTDTQASDGELGYGDTTTHTSRLPGVVTWLAAADSTVVRGKPLYRVDDDPVVLLYGSLPAYRTLSPGVTGRDVKQFEKNLWALGYRGFTVDRTYSSATAAAVREWQDDLGLGETGRIDPARTVYAPGAVRVDSRSAAPGDVAQPGTALLETTATGRVATVTLDISDARLARRGAPVEVTLPDGKEVRGLIKSVETVVTPAEGQQKASTSLTVTISFAKSPAGLDKAAVSVAFTAAKRENVLTVPVAALLALAEGGYGLQVVEGGSTRYVAVETGLFADGRVEVSGDGIAAGTSVGMPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRZ5</accession>
    <name>U5VRZ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mov34/MPN/PAD-1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_06340</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359088.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06340"/>
    <dbReference type="MEROPS" id="M67.009"/>
    <dbReference type="EnsemblBacteria" id="AGZ39557">
        <property type="protein sequence ID" value="AGZ39557"/>
        <property type="gene ID" value="AFR_06340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1286"/>
    <dbReference type="eggNOG" id="COG1310">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116765_1_0_11"/>
    <dbReference type="OrthoDB" id="1811367at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070122">
        <property type="term" value="F:isopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="2"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15533" checksum="B8E304B003727BD7" modified="2014-01-22" version="1">MLTIDSAIVDAIVAHARRDHPDEACGVVAGPIGSDLPTRHIAMDNAARSMTFYEFDSMEQLRVWREMDDNDEEPVVIYHSHTATEAYPSRTDISFAGEPGAHYVLVSTREPDSEEIRSFRIVDGVVTEEKVNIVDATVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS68</accession>
    <name>U5VS68_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07050"/>
    <dbReference type="EnsemblBacteria" id="AGZ39699">
        <property type="protein sequence ID" value="AGZ39699"/>
        <property type="gene ID" value="AFR_07050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1438"/>
    <dbReference type="eggNOG" id="ENOG5033BVN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_209514_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="48" mass="5112" checksum="91DDA4C829D7786D" modified="2014-01-22" version="1">MALLLWILAVILVVAGILALFRRQILWGVVLIIVGLLVGPGGVSIFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSD0</accession>
    <name>U5VSD0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359300.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07400"/>
    <dbReference type="EnsemblBacteria" id="AGZ39769">
        <property type="protein sequence ID" value="AGZ39769"/>
        <property type="gene ID" value="AFR_07400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1510"/>
    <dbReference type="eggNOG" id="ENOG5032Y20">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083318_1_0_11"/>
    <dbReference type="OrthoDB" id="1374065at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="22761" checksum="429A6C07A850FF10" modified="2014-01-22" version="1">MGAHGITGVADAGAFLARLVRLDRKLVVRLRSSAGERTALWAVLPWGVLVTREVAGPGPGDVTVAAADLLAALAEKSPELPSRRDSEWRWPLPPARSTAVESVAAGELSRLATAAAGTLREVTAGGLAGRAVGQRAVRDALLDHVALTVTPDDGRPVEVSQRLVQAVSKMGFLGPAEGDGPDARVCVAGRWVGLSAPYGVAWLQPVQVLTVMPIGGHPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSH0</accession>
    <name>U5VSH0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase</fullName>
            <shortName evidence="11">NN:DBI PRT</shortName>
            <ecNumber evidence="5 11">2.4.2.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">N(1)-alpha-phosphoribosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cobT</name>
        <name evidence="12" type="ORF">AFR_07365</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide = alpha-ribazole 5'-phosphate + H(+) + nicotinate</text>
            <dbReference type="Rhea" id="RHEA:11196"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15890"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="EC" id="2.4.2.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the CobT family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.21" evidence="5 11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359293.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07365"/>
    <dbReference type="EnsemblBacteria" id="AGZ39762">
        <property type="protein sequence ID" value="AGZ39762"/>
        <property type="gene ID" value="AFR_07365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1502"/>
    <dbReference type="eggNOG" id="COG2038">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002982_0_0_11"/>
    <dbReference type="OrthoDB" id="1765140at2"/>
    <dbReference type="UniPathway" id="UPA00061">
        <property type="reaction ID" value="UER00516"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1610.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00230">
        <property type="entry name" value="CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017846">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023195">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="SSF52733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03160">
        <property type="entry name" value="cobT_DBIPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="8 11 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11 12" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ39762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="350" mass="34568" checksum="2058A1CFAEE63A22" modified="2014-01-22" version="1">MTLETTLAAIGPADEPAMAAARDLQSRLTKPAGSLGALEELSVRLAGLAGVCPPPLPEPAAIAVFAGDHGVHAQGVTPWPQEVTAQMVANFLGGGAVINAFARQVGASLMVVDVGVAIPLHGGDTLLDANIRRGTRDLSVEPAMTTDEARAAVEVGISVAGALIDGGAKCLLTGDMGIANTTPAAALIAAFTGSDAAAVTGRGTGIDDKTHARKTAVVAAALQLHAPDPADPLVTLAALGGLEHAALAGFLLGAAERRVPVIVDGVIAASAAVAAAAFAPDAVTAMVAGHRSAEPGATVALEHLGLTPLLDLGMRLGEGTGAALALPLVAGAVRVLREVATFDSAGVSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSI0</accession>
    <name>U5VSI0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-like N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08320"/>
    <dbReference type="EnsemblBacteria" id="AGZ39953">
        <property type="protein sequence ID" value="AGZ39953"/>
        <property type="gene ID" value="AFR_08320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1693"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_4_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="19797" checksum="FF737FEF055773DC" modified="2014-01-22" version="1">MQPSDSTIIEFDSNQSEGSMVTVRAMSADDAEQVISIYQAGLDAGDASFEDTAPGWERFDAGKLPGHRFVAVDSTVLGWIAASAVSARPVYAGVVEHSVYVDPAAQRRGVGRALLDALITSTEAAGVWTLQTGIFPENTASLALHHAAGFRTVGVRERLGRHAAHNNRWRDVMFLERRSPYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSK2</accession>
    <name>U5VSK2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38690">
        <property type="protein sequence ID" value="AGZ38690"/>
        <property type="gene ID" value="AFR_02005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.406"/>
    <dbReference type="eggNOG" id="ENOG5033FKG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111536_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045773">
        <property type="entry name" value="DUF6226"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19736">
        <property type="entry name" value="DUF6226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17697" checksum="A7909E93E31FCB40" modified="2014-01-22" version="1">MHEAAETLLDELTERYVVDRREAKEPVGPEPGAEVVRTVRLVPRSPAAGPLSIAFTDFPGVVLRLGRWFLEPLPDCGCDDCGEDPALLVTDLRARVAAHVEGGLWERVRRGLTSSWLETRLIGSGLRTSRQAPIEAAEARAARREGFAAPVQWAPWPLRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VST6</accession>
    <name>U5VST6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase (ATP)</fullName>
            <ecNumber evidence="1">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_02060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357644.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02060"/>
    <dbReference type="EnsemblBacteria" id="AGZ38701">
        <property type="protein sequence ID" value="AGZ38701"/>
        <property type="gene ID" value="AFR_02060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.417"/>
    <dbReference type="eggNOG" id="COG1793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008325_4_0_11"/>
    <dbReference type="OrthoDB" id="1073940at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014146">
        <property type="entry name" value="LigD_ligase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02779">
        <property type="entry name" value="NHEJ_ligase_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_LIGASE_A3" evidence="3">
        <location>
            <begin position="106"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="34193" checksum="71E7617BAD8E8E50" modified="2014-01-22" version="1">MPGAPLSPMLATAGTLPRGPGWSYEFKWDGVRVLSSFSDGPPRLWARSGAAVTLAYPEVASLSLPPDSLLDGEMVVLDAAGRPSFTALAERMHVRDPGRAARLAATLPVTYMIFDLLRYDGESLLGLPYADRRDRLEKLDLGPGSWMVPPAFTDGAATEAAARENKLEGVVAKRLDATYLPGARSPDWIKVKFDRTGDYVIGGWRSGARKLGGLLVGVPGPDGRLRFRGRVGGGIGSASEQELLAALAPLVTRESPFATGAVPREDSRGAHWVSPDLVVEVRYGNRTPDGRLRFPRFLRLRPDKSPSECVEEDDDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSV0</accession>
    <name>U5VSV0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357743.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02555"/>
    <dbReference type="EnsemblBacteria" id="AGZ38800">
        <property type="protein sequence ID" value="AGZ38800"/>
        <property type="gene ID" value="AFR_02555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.514"/>
    <dbReference type="eggNOG" id="COG3266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036302_1_1_11"/>
    <dbReference type="OrthoDB" id="311112at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007795">
        <property type="entry name" value="T7SS_EccB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044857">
        <property type="entry name" value="T7SS_EccB_R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40765">
        <property type="entry name" value="PTHR40765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05108">
        <property type="entry name" value="T7SS_ESX1_EccB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03919">
        <property type="entry name" value="T7SS_EccB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="452" mass="47267" checksum="DC189936B9103A07" modified="2014-01-22" version="1">MQTQRDHVHAHTFMMGRLSSALVQGDPTSAEIPGRRAQTGLLIGIILVVLVAGGFAVYGWIVPGGSKAFRQAGAILVEKESGTRYVYLNGVLHPTPNLTSAMLIQGNAAKVKLISRNSLKDVPRGAAIGIAGAPQSLPAADALVPGPWLTCLPGSVIDNPGPQLGVNLDPRTDNAPLPGDRFAVAQNAKGVPYLLAEGRKFRVADDAVLVALGAANVRPIRAPQVWLDWLPDGEALAPAAIPGAGTKGPEVGGERRAIGTLFRQKPAAGEEQLFVLRRDGLAPMSRTEFLFADAKGAGAPVELDAAAVVDAPKSEDRSLTSRLPDLAGLRWQDPGRAVLCLRQQPASADSFSSTVVYVPQDRSAVDPDGRTSVYARPGTGMVVVAVPKESRITPEVSLISEEGTAFTLADSATVSALKFDKIGLVPFPKSLLATLPQGPLLSREAVSGLAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTC3</accession>
    <name>U5VTC3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative copper resistance protein CopC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_03110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357854.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03110"/>
    <dbReference type="EnsemblBacteria" id="AGZ38911">
        <property type="protein sequence ID" value="AGZ38911"/>
        <property type="gene ID" value="AFR_03110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.626"/>
    <dbReference type="eggNOG" id="COG2372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087859_0_1_11"/>
    <dbReference type="OrthoDB" id="1753926at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032694">
        <property type="entry name" value="CopC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007348">
        <property type="entry name" value="CopC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34820">
        <property type="entry name" value="PTHR34820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04234">
        <property type="entry name" value="CopC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665733" evidence="4">
        <location>
            <begin position="27"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="CopC" evidence="5">
        <location>
            <begin position="27"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="18981" checksum="7CF238FAEB6F07C7" modified="2014-01-22" version="1">MRLVRSVAAALVAATLAVLAGGPAWAHNSLVEAAPKKDAKLAKAPAEIRLKFIQKLDGRYMTIVVSDEAKQKVAVSAPKADGKVGSVTFSEPLPNGVYTVAYRVVSQDGHAVQGAYSFTVQAAGASTPSAVAPTTASPVAPASAAPVPVALVEDKTATWWPVATIAVAVLLVGAGAFVLVRRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTE2</accession>
    <name>U5VTE2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pyruvate dehydrogenase E1 component</fullName>
            <ecNumber evidence="2 8">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">aceE</name>
        <name evidence="13" type="ORF">AFR_08765</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H(+) + pyruvate = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10480"/>
            <dbReference type="Rhea" id="RHEA-COMP:10481"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="2 8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359573.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08765"/>
    <dbReference type="EnsemblBacteria" id="AGZ40042">
        <property type="protein sequence ID" value="AGZ40042"/>
        <property type="gene ID" value="AFR_08765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1783"/>
    <dbReference type="eggNOG" id="COG2609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009154_2_0_11"/>
    <dbReference type="OrthoDB" id="49937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02017">
        <property type="entry name" value="TPP_E1_EcPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035807">
        <property type="entry name" value="PDC_E1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004660">
        <property type="entry name" value="PDH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041621">
        <property type="entry name" value="PDH_E1_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825:SF3">
        <property type="entry name" value="PTHR43825:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17831">
        <property type="entry name" value="PDH_E1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000156">
        <property type="entry name" value="Pyruvate_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00759">
        <property type="entry name" value="aceE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 13" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="11">
        <location>
            <begin position="148"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="PDH_E1_M" evidence="12">
        <location>
            <begin position="494"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000156-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ40042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="912" mass="102134" checksum="AA8CDE3B63C2E1E0" modified="2014-01-22" version="1">MATERKRPVISDGLPSQLPDIDPEETAEWVESLDGVIDEGGAKRARYVMLRLLERARERQVGVPPLTSTDYINTISPEQEPWFPGDEFVERRIRAYIRWNAAMLVHRAQRPEIGVGGHISTYASSASLYEVGMNHFFRGKDHPGGGDHIFFQGHASPGMYARAYLEGRLSENDLDGFRQELSHPGGGLPSYPHPRLMNNFWEFPTVSMGLGPLNAIYQARYNRYLHNRGIKDTSQQHVWAYLGDGEMDEVESLGAIGLAAREELDNLTFVINCNLQRLDGPVRGNGKVIQELEAFFRGAGWNVIKVVWGREWDTLLAKDTDGALVNLMNVTPDGDFQTYKGESGAFVRENFFGRDPRTRKLVEGMTDDEIWGLKRGGHDYKKLYAAYKAATEHTGQPTVILAHTIKGWTLGSHFEARNATHQMKKLTLDDLKGFRDRLYLEISDKQLEENPYLPPYFHPGEKSDEMQYMLERRRELGGFVPTRRTKHKSLAIPESKTFGDIKRGSGKQKVATTMAFVRLLKDLMRDKEFGARWVPIIPDEARTFGMDSLFPTKKIYSPHGQTYTSVDRELFLSYKESTTGQILHEGINEAGSTASFIAAGTSYATHDEPMIPLYIFYSMFGFQRTADELWAAADQMTRGFLLGATAGRTTLNGEGLQHEDGHSLLIAATNPAVVAYDPAFAFEIAHIMENGLHRMYGEHQENVFYYLTIYNEPILQPVEPSDVDVEGLLKGIYKYSPAPETTGPKAQVLASGTGMQWALKAQQLLAQDWGVGADVWSVTSWTELRRDAMEIEEHNLLNPGDKPRKPYIETKLEGSEGPAIAVSDWMRAVPDLISRWIPNDYTSLGTDGFGMSDTRHALRRHFHVDAESIAVATLRQLALQGKVPAHVPAEAAKKYAIDDVNAAPVGETGGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTH6</accession>
    <name>U5VTH6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08965</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359613.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08965"/>
    <dbReference type="EnsemblBacteria" id="AGZ40082">
        <property type="protein sequence ID" value="AGZ40082"/>
        <property type="gene ID" value="AFR_08965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1824"/>
    <dbReference type="eggNOG" id="COG0057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030140_0_2_11"/>
    <dbReference type="OrthoDB" id="944149at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="PTHR43148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="6">
        <location>
            <begin position="3"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00030823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="353" mass="37497" checksum="0F6D50C0728A20F5" modified="2014-01-22" version="1">MSVAIGINGFGRIGRSVMRIIGANSDSGITVAAINDIAPVDRLAYALKRDSLRGRFGGTVAAGTGQLLVNNRPIWAFSESQPGNIPWAEAGVDVVIEATGRFRNGDLARDHITGGGARKVVISASADDPDAVLIYGANHERYDPLAHHVISPASCGVNALSVMAKVLLERFGLRSVHTSVVLAVQGWQKAQDAMTGSSRDDPRLGRATAESIIPHNHVAGDLLSVALPELGKVRYSYYCVPTPIGSLAELTGFTDRPVSREEVNRAMAEAAAGFMGGIIDYDPDPTVSIDVRDNPASCLFDPSGTQTTPDGGVQVRGWFDNEWGFSHRLLDLARLMGEREAVPVGAPYRSGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTI0</accession>
    <name>U5VTI0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cell wall synthesis protein Wag31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Antigen 84</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_10045</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DivIVA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360038.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10045"/>
    <dbReference type="EnsemblBacteria" id="AGZ40298">
        <property type="protein sequence ID" value="AGZ40298"/>
        <property type="gene ID" value="AFR_10045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2047"/>
    <dbReference type="eggNOG" id="COG3599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062236_0_0_11"/>
    <dbReference type="OrthoDB" id="875822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019933">
        <property type="entry name" value="DivIVA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007793">
        <property type="entry name" value="DivIVA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35794">
        <property type="entry name" value="PTHR35794"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05103">
        <property type="entry name" value="DivIVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03544">
        <property type="entry name" value="DivI1A_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="48"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="140"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="29535" checksum="E799DF98FFFB2784" modified="2014-01-22" version="1">MPLTPADVHNVAFKKPPIGKRGYDEEEVDAFLDEVERELARLIEENNELRAQVERGGRGGAPAGPSADPRLAAELNDMKAQLDRVQRDKSAAEQAARQMQAELEQVRAQGPVGAGAGAPTGADGEQQALRVLMMAQRTADDHVSDARREADKLLSDARSKAEEVTREARAKADALERDARQRHQEAMGGLDAKRSALQKHIEELKQFEREYRTRLKAYLESQLRDLDGRGQGLEAEMTRADANRSVGGSGGLAAAGLAGSYGGSRAGSIETGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTJ8</accession>
    <name>U5VTJ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03485"/>
    <dbReference type="EnsemblBacteria" id="AGZ38986">
        <property type="protein sequence ID" value="AGZ38986"/>
        <property type="gene ID" value="AFR_03485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.700"/>
    <dbReference type="eggNOG" id="ENOG502ZBAK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_402645_0_0_11"/>
    <dbReference type="OrthoDB" id="151976at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="626" mass="68872" checksum="E75AE1D9EEB7F8F1" modified="2014-01-22" version="1">MVGASAGAAGAAPKSSYHFLHPGGQPRLTEKVPVNVVFLGYEPGQVGKTAYLSGLPKKYEPIVRYRAVYGQTEKLGITYTYDYKVSYGTRSYEDRFFKQLGKLAKPAPLTQYQTAYNDQEKNVKDITSNNTIDAPSVEKWLAFNPPAGVDTRRNTIFFINWYGRSDFKFHVYTKTGEADPDTGYDFGKERDSRKIIAWGGTTADDEETGLGSTRRVWFHDLSAGPESWTTNYDVDNPDLDGDGVVDYRMPPTWEYTAGGFRAPSALAGDLGLITRYVAINLLFTSSPLYPAELPTAEPPKSINIDSNTYEGWPGVDASQQYIKPKLVVDELSELRWRNRLDFDNQDLPYDAKAQECYLGVLSDTSCYPDSGYPAFANLYLYNWANLDRTKDDDGRVDYELPVFNYAVGAGVGVPALGFADDNYVDGTQTGVFCFISPEVVQSGYGLSTTQIHEVGHHLGMSHPHDGYDSETGVDYEPGGEFLFANAGDESNSMMSYIDVNWDFSQFDRDNSDRFLTSAYNEAANRLAADVLGSGKESKVRGQLRAADVLLGLATTAFARHDYRAAYAFAEGAYNQVVAAAKRAGVDAAGPAKAMAAEADAARRSGEVHDPHEFIDTLAPDSPRSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTM7</accession>
    <name>U5VTM7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359749.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09315"/>
    <dbReference type="EnsemblBacteria" id="AGZ40152">
        <property type="protein sequence ID" value="AGZ40152"/>
        <property type="gene ID" value="AFR_09315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1898"/>
    <dbReference type="eggNOG" id="COG3694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071040_1_0_11"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010390">
        <property type="entry name" value="DUF990"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06182">
        <property type="entry name" value="ABC2_membrane_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="28283" checksum="92B6CF1A85761567" modified="2014-01-22" version="1">MAELRAYAALFGGQWRSLLSYRASFIVEMITNIGGTALDVLAVLVIFQATSTVAGFTLSQGLLIVSLSSCAFALADFTVGNIDRLKTYVRTGAMDAVLVRPLGVLMQLVLMDLPLRKALRLVVGFGVLAVALTVNPIDWTPARVTLLVITPLAGAVFFASIFVLSASLAFWWVDSGEVGSAFTYGGRDFTAYPTPVYAGWFRAVFAYGLGFGFVAYQPALALLGRADPLGLPTWAGFVSPLVALVAAALAAAVWRSGIRHYRSTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUG9</accession>
    <name>U5VUG9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360468.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11150"/>
    <dbReference type="EnsemblBacteria" id="AGZ40519">
        <property type="protein sequence ID" value="AGZ40519"/>
        <property type="gene ID" value="AFR_11150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2271"/>
    <dbReference type="eggNOG" id="COG1168">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_15_2_11"/>
    <dbReference type="OrthoDB" id="1462898at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="56"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="376" mass="40348" checksum="13FAC73D3D3B3384" modified="2014-01-22" version="1">MHVDPLETLRRRRSVKWRTYPADVLPLFVAEMDYALAPPIAAALRAAVDASDTGYSAAQPDLGIALSGFAARHWDWELDPASVTAVTDVGVGVVELLRLLVRPAGSVVISPPVYPPFFDWPPEAQAQVREVPLTAEFHLDLPALEQAFAAHPAVYILCNPQNPVGRVHTRDELTALVRLARLYGVTIVSDEIHAPLVLPGAEFTPLLTVPGAGQVAITVASASKGFNLAGLKCAAIVTGSPAMAAVVDRLPPDVRWRTGHLGVIATVAAYTEGDDWLASLHTALDARRTQLGELLRTRLPTISWRPPEATFLAWLDCRALGADDEPQQLFLTSAKVALEPGTRFGAAGAGYVRLNYATSPDILDEATARMASTTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUY1</accession>
    <name>U5VUY1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative IclR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12585</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360862.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12585"/>
    <dbReference type="EnsemblBacteria" id="AGZ40803">
        <property type="protein sequence ID" value="AGZ40803"/>
        <property type="gene ID" value="AFR_12585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2556"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_6_0_11"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="5"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="68"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="256" mass="27456" checksum="4DBB2C716676461D" modified="2014-01-22" version="1">MPRVVPAVIRALDVLELFLDRPELSARDVMERLDLPRTTVHELLVTLEARSYLISVPGQPVRYRLGMPVFQLGAAFAGRLDLVREAQDVARDVAAACDEAVHVAVLDGADVIYLVKVDSTHPVRMVSAVGRRLPAHCTAVGKILLSDLDQAALDDILAKGSLPGMTPTSITDPDLLRAQLEEVRAEGVAVDNGESDSAMRCVAAAVRDHSGAVVAAMSVSAPTIRWTAESHVEWTELVRDGAARLSAHMGHVAPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV03</accession>
    <name>U5VV03_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">YCII-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360748.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12010"/>
    <dbReference type="EnsemblBacteria" id="AGZ40689">
        <property type="protein sequence ID" value="AGZ40689"/>
        <property type="gene ID" value="AFR_12010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2445"/>
    <dbReference type="eggNOG" id="COG3795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130902_3_0_11"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="33"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13311" checksum="32EC9D2ADFE9B091" modified="2014-01-22" version="1">MARYLISFDDGAMDHIPDGDWAEVGKTSHAVIEEAVTAGVWVFSGGLQRQRASIVGTDGAVTDGPYPETKEVIGGFAVVDVATREEALEWAAKIAAGCRCAQEVREIMDDPETDDMLRRIGGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVP4</accession>
    <name>U5VVP4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360941.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12980"/>
    <dbReference type="EnsemblBacteria" id="AGZ40882">
        <property type="protein sequence ID" value="AGZ40882"/>
        <property type="gene ID" value="AFR_12980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2636"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_17_1_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="187" mass="20061" checksum="2840335043AE10D7" modified="2014-01-22" version="1">MTPRSTALRADAQRNRGHLIATAAEAFASGQTISLDAIAKRAGVGNATLYRHFPTREDLVEEVYRDQIRPLRDDAQALLATEQPAQALHAWMRRFAEWAGERRGICEALVAMSASGRFGTGPVCDEVQQILGMVLAAGAEAGELRSDIDPVDVGGILAGVLSVAGGTDQRPQLDRMLTIVVDGLRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVS7</accession>
    <name>U5VVS7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361122.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13885"/>
    <dbReference type="EnsemblBacteria" id="AGZ41063">
        <property type="protein sequence ID" value="AGZ41063"/>
        <property type="gene ID" value="AFR_13885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2818"/>
    <dbReference type="eggNOG" id="COG1262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004327_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025139">
        <property type="entry name" value="DUF4062"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005532">
        <property type="entry name" value="SUMF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042095">
        <property type="entry name" value="SUMF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13271">
        <property type="entry name" value="DUF4062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03781">
        <property type="entry name" value="FGE-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="SSF56436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="252"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1048" mass="115922" checksum="4C267BE7B7321675" modified="2014-01-22" version="1">MKIFLSGVSSEFRECRTSLASDLRAMGAEVMLQEDLQQGGRSLLEKLQSYIEFCDRVIYLLGDTYGYEAPISGGGPAPRSYTQWEYFFAMGEKLDGRRAPAKEIYLYVADAKFHPQSRLIQSEEHERRQKAFADMVLSSGYDRGRFSSLDALCRLALRDGFHLPTLPQTSPDVSSVHEYLRSLRSRLDQIDIGSPVQRVNSVATPLRHLNIIEAVCTRPRVRRILKLADSPDGIEVSETQREAFAGIGDAGRRQVILGHPGSGKTTLARWLVIRYAQDFLEGRSFSVPIYLPLREVALSSIGSLDDLIETATKLQPTLPAEAEVRRFLRENRNRLSLIIDGLDELPAISAAGEARAESISRLYGILDEFIDSSEGHQVVITSRIQDYLHNPEFQYSGAVCLLVDEMSIDQIKESILNWHRMAGRLASGDPDNADWLDRASRVFSLVQGNTDLASFVANPLCLNLLQMVYDDSELPYLSISRICEMAVRTLLRLRVEPKAPARDMPTDISHEQYLELLLSVLSDIAVAATDSIAGGGDGTVPLDEVQARVSACLGATTAKGFLARGALIHPIMLDLVRGHGVLISMNAQCFMFAHALFRDTLVGRALTQRGQTEYRDRASSLPWSKAFRHVAGLALADRGPSLAAVDLANALFQDYTRSRDTVMLASAAEIVGELNISSDVLSGALFGPFLAQIRVSLLEAATDRTAPAERRLLLGMLLGRVGDPRLAGTLRANNGQADGKFVRLPAAKVTLGAAMEPAITNPKYVSVFPRLVENLAFEEFSISRFLVTNAEYADFVSDGGYTTDVYWSDNGGVQWLHADGATLDRLRELMRTTSMTHFYTDLNNGRVSEAEISDAIDAMLLRKLPLYWFNHRLNGPNQPVVGVNWWEAKAYARWYSLRRAGHDASSPVVVDLPTEYEWERAARLGPRNDTDTIWAAGSATENAHWNALHRLPPGSAPVGLYPWGSPAMEGPHDLIGNVWEWTTSTNRPYAVAYIHQADDGYGLADRIVRGGSWLSSESLSSTISFRSFDPPCNAYDDLGFRIAATRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWE0</accession>
    <name>U5VWE0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LysR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_14460</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361233.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14460"/>
    <dbReference type="EnsemblBacteria" id="AGZ41174">
        <property type="protein sequence ID" value="AGZ41174"/>
        <property type="gene ID" value="AFR_14460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2935"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_39_0_11"/>
    <dbReference type="OrthoDB" id="1591366at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08417">
        <property type="entry name" value="PBP2_Nitroaromatics_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037402">
        <property type="entry name" value="YidZ_PBP2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="334" mass="35910" checksum="944EEE87EDB381A5" modified="2014-01-22" version="1">MRTASIDLNLLAALDALLAERNVTRAGRRLGMSQPAMSEALARLRRHFGDQLLVRVGNNYELTPLGAGLRSTCAAAMQLVEQTFTAAQGFDPEACEREFVLLASDYAAAVLGPPLMRELRQASPVASLRLDRLPTDCPPGAAPGRIRVPDGLVLPRSLAPPGLPGTTLFSDRWVCLAAADNPAVTGGLTVETLAALPWVLHQDMHADHPVLTNLRARGLRPEARFSVGGFHLLPELVRGTDHLCLIQERLAARVAGPDLCVLEVPFDLPPVVETLWWDPAQTHDPSHRWLRDLVAAAARGLTAEPGPVATPATSCKARPPGRTLSPSKRRSPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWF4</accession>
    <name>U5VWF4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41107">
        <property type="protein sequence ID" value="AGZ41107"/>
        <property type="gene ID" value="AFR_14125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2866"/>
    <dbReference type="eggNOG" id="ENOG5033H6F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180533_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="10887" checksum="6709EEE7F6D9EBF9" modified="2014-01-22" version="1">MEVWTPSDGYAAVMPLDPQPRGRRGTVGHAYSPLNLRLVLAAFVSVVSVVFAVLLLQTGVTVFGWLFVALAVVAAVDLVVVQLRRRARRRAEGDGHSLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWH3</accession>
    <name>U5VWH3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361417.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15380"/>
    <dbReference type="EnsemblBacteria" id="AGZ41358">
        <property type="protein sequence ID" value="AGZ41358"/>
        <property type="gene ID" value="AFR_15380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3123"/>
    <dbReference type="HOGENOM" id="CLU_1363797_0_0_11"/>
    <dbReference type="OrthoDB" id="2027598at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="21354" checksum="F221BBBF9093FBB8" modified="2014-01-22" version="1">MIEVVRSELYRMSTVRSSAMSLAVFTAAGILLSLVGSDFWALLAGVGAFGFGVTGVSQHYQHRTAVLLYLARPRRLRVLLGQLVTALIVSVGFTAVSGGVVFVQGSRERFLITLVVAPILALLGAAAAAIVRSATFLFVGFAVWVIFVEAMYGKMQEPLPFSAYLDAATGDVKKLLILLGWTLATIVGAAFAIRRDLNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWP6</accession>
    <name>U5VWP6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_09205</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359706.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09205"/>
    <dbReference type="EnsemblBacteria" id="AGZ40130">
        <property type="protein sequence ID" value="AGZ40130"/>
        <property type="gene ID" value="AFR_09205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1874"/>
    <dbReference type="eggNOG" id="COG4941">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_417781_0_0_11"/>
    <dbReference type="OrthoDB" id="1469807at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="8">
        <location>
            <begin position="412"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ40130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="656" mass="71473" checksum="A65FB258B1C99514" modified="2014-01-22" version="1">MTDTHRTIDAVWKLESARIIGGLMHLVHDIGLAEELAQDALVAALEQWPAAGVPENPAAWLTAIAKRRAVDHLRRARLHERKADAYARDLDRPAEPDHDDVLRLMFISCHPVLPAQDRAALTLRVVGGLTTKEIARAFLTSEPEITRRIADAKRALADAAVPFALPEPEELTGRLSSVLEVVYLIFNEGYSATSGDDLMRPALGHEALRLGRMLAELAPDQAEVHGLVALMEIQESRSAARTGPAGEPIQLHEQNRGRWDPLLIRRGFTALLRAKDLGGTPGPYVLQAAIAVTHAQARTAEETDWAGIAGLYAALARLLPTPVVQLNRAVAVGLARGPQAGLDLVDSLRTDAALRAYHLLPGVRGDLLLRLGRDEEARHEFERAAALARNAAESAFLLRRAAEIPAPAPAGSTLGEAVRDFLARDDLGPATLRSYRQTLERLSLAVGGGLPLTTVTAPEVARAFSSLWGSTSAKTWNRHLSAIRSFSTWSATGDLAATLERRPETRPRITPITATGLEAVWGLDLPLRERALWRLLHESAAGVRTVLSLNVEDLDLEDRRARAGATWVTWRSGAARLLDELLGGRTRGPVFLADRRPGPGRMPPEADRCPDTGRGRLSYERAEYLFKKATGPLDPDGRGFTLRRLKPFRDAGPGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWQ8</accession>
    <name>U5VWQ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coenzyme A biosynthesis bifunctional protein coaBC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359597.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08885"/>
    <dbReference type="EnsemblBacteria" id="AGZ40066">
        <property type="protein sequence ID" value="AGZ40066"/>
        <property type="gene ID" value="AFR_08885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1809"/>
    <dbReference type="eggNOG" id="COG0452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118224_0_0_11"/>
    <dbReference type="OrthoDB" id="1429052at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="1">
        <location>
            <begin position="12"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="21058" checksum="1F8938D75A9C7D9C" modified="2014-01-22" version="1">MSVNTSPGVLYVLVCGSPIARDVGVLVDLAQRDGWEVCVVTTPDGRKFVDAAALHAQTGHPVRSYYKNPGDEDLLPPADAMIVAPATVNTINKWSAGITDTLVLGLLIEGYGFGIPTVVVPYTNKVMALHPSLHDSIAKLRNWGVQVLYGDDVLRLGGPGQNERYRSQFPWRRALQALHTRFAADNRVEPGARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXA5</accession>
    <name>U5VXA5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="10">2.4.1.227</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">murG</name>
        <name evidence="13" type="ORF">AFR_10010</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-glucosamine = di-trans-octa-cis-undecaprenyl diphospho-[N-acetyl-alpha-D-glucosaminyl-(1-&gt;4)]-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:31227"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="ChEBI" id="CHEBI:61388"/>
            <dbReference type="EC" id="2.4.1.227"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 28 family. MurG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.227" evidence="10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360025.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10010"/>
    <dbReference type="EnsemblBacteria" id="AGZ40291">
        <property type="protein sequence ID" value="AGZ40291"/>
        <property type="gene ID" value="AFR_10010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2040"/>
    <dbReference type="eggNOG" id="COG0707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037404_1_0_11"/>
    <dbReference type="OrthoDB" id="1165736at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051991">
        <property type="term" value="F:UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050511">
        <property type="term" value="F:undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00033">
        <property type="entry name" value="MurG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006009">
        <property type="entry name" value="GlcNAc_MurG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01133">
        <property type="entry name" value="murG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="9 10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="11">
        <location>
            <begin position="7"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_tran_28_C" evidence="12">
        <location>
            <begin position="197"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-N-acetyl-alpha-D-glucosamine binding" evidence="10">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ40291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="39415" checksum="A86BD5F46E60756E" modified="2014-01-22" version="1">MGLLRSVVLAGGGTGGHIYPLLAFADALRRHFPEVRITTLGSPKGMENELIPPAGYDLRVIPAFQLPRSINLNLLRTPDRMYKSAHAAGQILDEVQADVVVGFGGYVSVPAYLAAWRRELPIVIHEVNVPPGVANRMGMKFTKNIAVGFPTQPQQAESLKDARVVGVPLRTAIARMDRQALRPQALAHFGLRPDLPVLFVSGGSSGARTINLAVAGAAKKLAHAGIQVLHVQGGRNDPFEVPSDLPVPYVVVPYLSDMQLGYAAADLMLCRAGAITVAETTAIGMPAVYVPYPFSNQEQKRNALPVVEAGGGVLVDNAELTPEWIERTIIPLARDPQRLATMGAAAGSYGRRDGDEALLDFVCEAVAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXA7</accession>
    <name>U5VXA7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361486.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15725"/>
    <dbReference type="EnsemblBacteria" id="AGZ41427">
        <property type="protein sequence ID" value="AGZ41427"/>
        <property type="gene ID" value="AFR_15725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3193"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_914757_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="33392" checksum="172AEFC8921A7B22" modified="2014-01-22" version="1">MAFESTDLRSTLPSAAAAPATPERFSGSEHVQFRDVPPVEKTETISTWYARGQNFVVGYSDLDGTAALSRTSQPDEYVVLLADDDLEARVEAPGEELTVSGRTMIVVPPGDSTVTITGRGRVLRLLTTKSEDIAALAANAGSYAEAHENIPPFQPWPDPVGGFRVRTYDLTVAPLANPPFRLYRCTTFMVNFIDPKQGPRDPAKLSPHKHDDFEQCSIVLAGEYVHHLRWPWTTNKANWREDEHYTVVAPSVTVIPPPSLHTSEAVGAGTNHLIDVFCPPRFDFSDMEGWVFNAADYPAPEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXK8</accession>
    <name>U5VXK8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable cell division protein WhiA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">whiA</name>
        <name evidence="8" type="ORF">AFR_10560</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in cell division and chromosome segregation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WhiA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360241.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10560"/>
    <dbReference type="EnsemblBacteria" id="AGZ40401">
        <property type="protein sequence ID" value="AGZ40401"/>
        <property type="gene ID" value="AFR_10560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2153"/>
    <dbReference type="eggNOG" id="COG1481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053282_0_0_11"/>
    <dbReference type="OrthoDB" id="1634356at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043937">
        <property type="term" value="P:regulation of sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01420">
        <property type="entry name" value="HTH_type_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018478">
        <property type="entry name" value="Sporu_reg_WhiA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003802">
        <property type="entry name" value="Sporulation_regulator_WhiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023054">
        <property type="entry name" value="Sporulation_regulator_WhiA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039518">
        <property type="entry name" value="WhiA_LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37307">
        <property type="entry name" value="PTHR37307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02650">
        <property type="entry name" value="HTH_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14527">
        <property type="entry name" value="LAGLIDADG_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10298">
        <property type="entry name" value="WhiA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00647">
        <property type="entry name" value="DNA_bind_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WhiA_N" evidence="6">
        <location>
            <begin position="19"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="LAGLIDADG_WhiA" evidence="7">
        <location>
            <begin position="128"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="HTH_WhiA" evidence="5">
        <location>
            <begin position="221"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="34924" checksum="947EF377B64AEF31" modified="2014-01-22" version="1">MAMTAAVKDELSRVDVPKPCCRRAEMAALLRFAGGLHIVSGRVVVEAELDTGAVARRLRKEIAEVYGYPSEVHVLASGGLRKGSHYIVRIVKDGEALARQTGLLDVRGRPVRGLPPHVVSANVCCAVAAWRGAFMAHGSLTEPGRSSALEITCPGPEAALALRGAARRIGITAKEREVRGVDRVVIKDGDAIAALLTRIGAHSSVLAWEERRVRREVRATANRLANFDDANLRRSARAAVAAAARVTRALEILADEAPNHLTSAGRLRLEHRQASLEELGALADPPLTKDAIAGRIRRLLALADKRARDLGIPDTEAAVTPEMMAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXL2</accession>
    <name>U5VXL2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Two component signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361607.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16250"/>
    <dbReference type="EnsemblBacteria" id="AGZ41532">
        <property type="protein sequence ID" value="AGZ41532"/>
        <property type="gene ID" value="AFR_16250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3297"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021898_1_0_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="452"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="640" mass="66816" checksum="C030619EFD75E5FD" modified="2014-01-22" version="1">MTRTAMLSSVAGAATAVLVAGGFALGLHVGNLHNGLIAASFTAVGLFVLSRRPGNREGLLFLVTGTAHAVMFAGRQYALHDGPLPGASWAGWLGVWPLPLVLFAAGLTLMCFPDGRLPSRGWRPVAAVLAVVGVALSAVSALWPVEYARTGLVAGHPLDLPGFAAADAFYSVARPAGYLLFQLTWAACVVVRLRRARGDEARQLRWFVYAVTLTAAIMVVGLAVWGSPVPGALAMPAVAVVAGAAILKYRLYDIDLVINKSLVLGAMATIVTLGYVAVVTGVGRLVGGNRTVLSVVATAIVAVAFEPLRRRVQRLADRIVYGHRVSPYESLARLSAHLAAPDAGLLLDGICRTVADGVGAREVVLSTGPPDQPQVVSRWPDTPAPRRPGEVVTVPVRHDGRALGTLTVRKVSGEALSGTERKLVGDLAAQAGLVLELRATAQRLVTSADAARRRLERDLHDGAQQRLVTVAMELGAVVRLARDSGAAQLADRADSVRAQLLEATADLREMARGLHPAVLTQDGLEAALGFLADRSPVPVRLGVTVDRRLRPEVEATAYFMVSEGLTNTAKHASAGSASVRAELADTFLIVEVADDGIGGATMPPGSGLEGLADRLAALGARLTMDSGPSGTRLTTVIPCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXP2</accession>
    <name>U5VXP2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360311.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10735"/>
    <dbReference type="EnsemblBacteria" id="AGZ40436">
        <property type="protein sequence ID" value="AGZ40436"/>
        <property type="gene ID" value="AFR_10735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2188"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_7_0_11"/>
    <dbReference type="OrthoDB" id="1122642at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="25326" checksum="F7FA81447B70051A" modified="2014-01-22" version="1">MGVVAELILIRHGQSAANVAFPLADEKGLLESGLSGRDTDVELTETGVEQARAVGRWLAGLPADQVPETVITSPYLRARETWRIAAETSGLSFPEPSTDDRLVDRLLGDLEMLTRAAVDQRFPDERARWEQATEYTYRPPGGEDFADIAARLTSFLDDLNATHAGERVVVVAHDSVVLMMRYVIEGLDWTRLAAVVAEAGRVLNASISRFDGSSGRLVLDHYNSVDHLPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY64</accession>
    <name>U5VY64_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH azoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361919.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17220"/>
    <dbReference type="EnsemblBacteria" id="AGZ41722">
        <property type="protein sequence ID" value="AGZ41722"/>
        <property type="gene ID" value="AFR_17220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3492"/>
    <dbReference type="eggNOG" id="COG0431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_4_3_11"/>
    <dbReference type="OrthoDB" id="1818437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="22000" checksum="86BD05D2A6E0F6F0" modified="2014-01-22" version="1">MTRILLISGSTREGSLHTSALRAAARLAPPGISTILYDQLRHLPAFVPGEPALPGAVTALRQSVEEADAVLFSTPEYAGSLPGTLKNLLDWLIAGGDLVGKPAAWLSVAAPGQDDGALAALEAVLANGNARLLKAACIRVPVSPEAVDADGVVDDPRLTMALTDTLRALGRLLAVPDPRKQPNWQAYSSVFPVVMRRDPTVTRHGRHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYC7</accession>
    <name>U5VYC7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Daunorubicin resistance ABC transporter ATPase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_18080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362262.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18080"/>
    <dbReference type="EnsemblBacteria" id="AGZ41894">
        <property type="protein sequence ID" value="AGZ41894"/>
        <property type="gene ID" value="AFR_18080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3669"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DUF4162"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DUF4162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="7"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="32991" checksum="0E3A13D02C770F37" modified="2014-01-22" version="1">MTTEHALVAQGLRKRYGDLHALDGFDLTVAAGTIHGLLGPNGAGKSTAVRALATLIDLDGGTARVAGLDVRRQPKQVRTRIGLVGQHPAVDEILGGRQNLVMFRRLYGLSTAAAKTRAGELLDAFGLAAAAERAVSTYSGGMRRRLDIAAGLILRPAVLFLDEPTTGLDPRARNEVWETVREVAGLGTTVLLTTQFLDEADQLADGISVLDHGRVVASGSPDELKRRLGGDRVTVTFPRHEPLRTLIDGPVSMDEEAHTATYASGSGVVLLAELVRALDRAGITPDDLVLRRPTLDEVFLSLTTEEANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ33</accession>
    <name>U5VZ33_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADP oxidoreductase, coenzyme F420-dependent</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360995.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13250"/>
    <dbReference type="EnsemblBacteria" id="AGZ40936">
        <property type="protein sequence ID" value="AGZ40936"/>
        <property type="gene ID" value="AFR_13250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2690"/>
    <dbReference type="eggNOG" id="COG2085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076368_0_2_11"/>
    <dbReference type="OrthoDB" id="1768241at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="1">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17030" checksum="E7B833561749E7DC" modified="2014-01-22" version="1">MTTIGILGAGQAGSTLARAAIAAGYDVVIANSRGPGTLAALIDELGPHARAAHATDAAAAADFVVTAFPYAPGDKLPVEALAGKVVLDTNNYMAWRDGNYPEVDSGLKTIHELRQEQLPTAKVAKAFTHIQFHARSPVRVDGQSRDELIRNLQSARRTPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ46</accession>
    <name>U5VZ46_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_19905</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362625.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19905"/>
    <dbReference type="EnsemblBacteria" id="AGZ42253">
        <property type="protein sequence ID" value="AGZ42253"/>
        <property type="gene ID" value="AFR_19905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4039"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012341_2_0_11"/>
    <dbReference type="OrthoDB" id="856767at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038766">
        <property type="entry name" value="Membrane_comp_ABC_pdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30287">
        <property type="entry name" value="PTHR30287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666226" evidence="7">
        <location>
            <begin position="23"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="712"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="766"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="802"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="8">
        <location>
            <begin position="296"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="8">
        <location>
            <begin position="742"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="838" mass="84291" checksum="81BBF959FAF8D993" modified="2014-01-22" version="1">MVRLAMKMAAQRITALLAVACAVLGGAALVTATGVISESGLRSHLPPGRLAGADVVVTAPQTARVGGDLPLALPERAPVPAGVAERLGRVPGVETVVADVSFPAALVVQGHVLPGEAGHGWSSTTLLADPRIEGSAPNRPDEVAVDSAAGISVGDPVEIVAAGRPARYRVSAVVSGAAGGIYFADTTAAGLAGRGTAAPDRTGSAAQPGLSAGPDLVGLRAAPGQADRVASAVRAELRGTGLTVATGSTRGDAAAPEAGAARQLLLLIAGSLAGIILIIIGFVVAGALGVALSGQRRDLALMRAVGATPRQIRRLAAGQALTVTAVALVPGVALGYPLAGQFGRLLARLGLLPDGLPLSLSPLPAIAAVLLLGLVVGVSAVSSAWRLSRMAPTEAVAESRTEPRALSPVRTTAGMLLMFGGTALSVVPLLVRTPVGAATTSMAGILGAVGLALAGPALVRRAGDALGKRLPRRASAPTWLAVANVRGYVLRVGGVVASLAMVLVFVLTYALTQTTVQAAADRETRAGTVAQHTIAAPALGGVPVGLEDEVRSTAGVEAVSSLTTTTVLWPYRMFGEEDVESHTAMVLTPAAADVLDLGVTGGSLQGLTGATIAVGSDVAGSRDAEVGRTVALVLGDGARVEAKVVAVYDRELGFGPLVVSRDLVAGHTSTGLDASLLVRADSTDALTALVAGRPGLTLGSARPEASDTPPEAWINLALVVVLLAYLLLSIANKLVAATAQRRTEIAVLRLSGTTPRQVRAMIRREAALIAAGALVLGLALAAVPLALLGIGFLDRPWPAGPVWLLPAAVLTVVVTAFLTMELPARRALRVPPAEALRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ76</accession>
    <name>U5VZ76_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362541.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19485"/>
    <dbReference type="EnsemblBacteria" id="AGZ42169">
        <property type="protein sequence ID" value="AGZ42169"/>
        <property type="gene ID" value="AFR_19485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3954"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148021_1_0_11"/>
    <dbReference type="OrthoDB" id="1900922at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="2"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="114" mass="12575" checksum="96C45690F6BE2119" modified="2014-01-22" version="1">MTVDLFAGIPVRDYATAVAWYERLLGGPPSFLPNDTEAVWELAENRYVFIEVRPAHAGHAMHTVFVSDFDTRIAEIAARGLEPADRETYENGVRKATFRDPDGNEIGLGGAPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZB8</accession>
    <name>U5VZB8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19150"/>
    <dbReference type="EnsemblBacteria" id="AGZ42102">
        <property type="protein sequence ID" value="AGZ42102"/>
        <property type="gene ID" value="AFR_19150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3889"/>
    <dbReference type="eggNOG" id="COG3733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092077_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665908" evidence="2">
        <location>
            <begin position="26"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="24403" checksum="68C241A88398778A" modified="2014-01-22" version="1">MSWGLGISTVTALAAFGVASWQANAAPPSPGGPGVAAEQPRVSDPSPAPEQATGTGSDALTTDEVGRARAVALAPLTGGTEDVTGAAGPEYLSAELVTDGAQRRADVYYYDYRAEKLIKQVVDLGTGKVTGSYAAAGMQPPAADREVGTALDLVLASPHAAELRDGYARATGRTYSGKDDIVVTAHVYKARPADTAVRNCGKHRCLQLIVQAADGPFIDLNQIIIDLSGRTVARLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZY6</accession>
    <name>U5VZY6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_20885</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20885"/>
    <dbReference type="EnsemblBacteria" id="AGZ42449">
        <property type="protein sequence ID" value="AGZ42449"/>
        <property type="gene ID" value="AFR_20885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4234"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="23"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="119"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="190" mass="21156" checksum="C52B8212A75EA446" modified="2014-01-22" version="1">MNPDDELAERFRAGDEHALRTAYDRYGGAVLYLAVRMLGNRADAEDVTQTTFVAAWQGRDTFDPERGGMLGWLLGIARRKTVDRLRSAAREERVATTVRAQPVADEESPERVLDRLVVADELAQLPVEQRRTIELAFYDDLTHPQIAAMTGLPLGTVKSHIRRGMANLRRRWEVDGAASGTRSAGPSRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W070</accession>
    <name>U5W070_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_15465</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361434.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15465"/>
    <dbReference type="EnsemblBacteria" id="AGZ41375">
        <property type="protein sequence ID" value="AGZ41375"/>
        <property type="gene ID" value="AFR_15465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3140"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_11"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="225"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="282"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="540" mass="55409" checksum="1929B29326C7DD90" modified="2014-01-22" version="1">MTTSAPSAAVPGRRGFRDLGTGVKIFTAVAVAALVALTVGVVGLLSLSRASTAADQIYTRNVSGVSAISRVRAEVLKARLDVSNQALALDTATTQKYETAFATAVGDFEKALKDYEQTSDTENPAAVADLQKQWQAYSSIVKTELLPAGATHDIATWEKIRDARVLPIMDRINTTVDDMAAAETAEAAAAAESARQAYRSSVTMSVSVLVAGLLAAILIAVLVTRGIVRSLARIEQVSEALAEGDLTRTSGLTSRDETGRVGQALDAAVARLRETVATINGSASSLAGASEEMSGTARKIAASAEETSAQARAVSTAAEEVSASVGTVSAGGEEMAASIHEISLNASEAARVAADAVAAAAVTSATMNQLGESSTEIGNVVKVITSIAAQTNLLALNATIEAARAGEAGKGFAVVAAEVKDLAQETARATEEISRRVATIQHDAAGAVTAIEGISEVIGRISDFQTTIASAVEEQTATTSEMNRSVAEAASGSGEISNTISGVAEAARLTSEGVAETRHATAELARMSTELTTLVSTFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0H8</accession>
    <name>U5W0H8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose reductase</fullName>
            <ecNumber evidence="2">1.1.1.133</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_16040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57510"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="1.1.1.133"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the dTDP-4-dehydrorhamnose reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.133" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16040"/>
    <dbReference type="EnsemblBacteria" id="AGZ41490">
        <property type="protein sequence ID" value="AGZ41490"/>
        <property type="gene ID" value="AFR_16040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3255"/>
    <dbReference type="eggNOG" id="COG1091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045518_1_2_11"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="PTHR10491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01214">
        <property type="entry name" value="rmlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD_sub_bind" evidence="3">
        <location>
            <begin position="11"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="290" mass="30220" checksum="A8496AAE17D18901" modified="2014-01-22" version="1">MGAAEGRVSLMRWLVTGAGGMLGRDLRTVLAEAGETDVVAATRADLDVTDQSAVRAAVAGADVVLNTAAWTDVDGAETAEEAATAVNGHAVHTLAAAAGKHLIHLSTDYVFAGDATAPYPEDAVPAPVNAYGRSKAIGEQAVLAAGGYVVRTAWLYGAHGPNFVRTMLRLAAERDTLDVVDDQQGPPTWSYALARQLVDLAGAARDGRAAPGAYHGTAAGSTTWYGLARAVFEEAGLDPDRVRPTTSATFRRPAPRPSYSVLGHAAWSRSGVDPLPDWRSMLTSAMPTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0R8</accession>
    <name>U5W0R8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363095.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22270"/>
    <dbReference type="EnsemblBacteria" id="AGZ42724">
        <property type="protein sequence ID" value="AGZ42724"/>
        <property type="gene ID" value="AFR_22270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4514"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_2_1_11"/>
    <dbReference type="OrthoDB" id="785019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665422" evidence="1">
        <location>
            <begin position="24"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="NMT1" evidence="2">
        <location>
            <begin position="70"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="332" mass="34861" checksum="A53B0F73CFACFD01" modified="2014-01-22" version="1">MKRLAALVALTLAFGIAACGSSASPASSDEITLKVGATGWAQAAAALEVAKLDDTPYKVEWPVFPGGDKQMQALTADAIDVGQASEIPPIFVAAAGGTDIKVIAVQRASTLLQEVIVGKDSTITDIAGLRGKKVGYVKNTTAQYFLYELLRQAGLGWRDIQAAPLLPKDGVAALGSGSIDALASYGNSIITARQGGARTIGTGKDILSGNFQWLAREKLLDDPAKSSAVVDLLARLHRAYAYIRDGHQQEYAQAVSDGTHQPVAEALQQLRDQEAQRPTVLQPTSAEAIKSQQAVADAFTDLGAIPGKLDVATIWTDRLNTELAAELAEPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1E6</accession>
    <name>U5W1E6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CDP-alcohol phosphatidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363347.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22860"/>
    <dbReference type="EnsemblBacteria" id="AGZ42842">
        <property type="protein sequence ID" value="AGZ42842"/>
        <property type="gene ID" value="AFR_22860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4633"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098617_0_0_11"/>
    <dbReference type="OrthoDB" id="1938728at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="20659" checksum="A536F1DCAE264263" modified="2014-01-22" version="1">MTALGLSPPVTLLNVPNTITAVRTAAAVGLAVAALTHRSAHLLVAAYLIYWIGDILDGAAARALGQETRTGAVFDIVADRACTSACAAALVVLRPDTALPIAVFLVQFMVIDQLLSLMFLRWPLLSPNYFARADRRIHRWNWSPPAKALNTAAVVLLALFAPAPAAVTVALAVTVVKVASLIAAARLPADLPLNRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W210</accession>
    <name>U5W210_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18745</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362398.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18745"/>
    <dbReference type="EnsemblBacteria" id="AGZ42025">
        <property type="protein sequence ID" value="AGZ42025"/>
        <property type="gene ID" value="AFR_18745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3806"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_11_1_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045823">
        <property type="entry name" value="TetR_C_32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19344">
        <property type="entry name" value="TetR_C_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="23"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="24024" checksum="5C8ED57AC689A64D" modified="2014-01-22" version="1">MNSADEGVNRPDGRSRRWADHRERRRTELVEAVIEAIRELGPEPGIDAVAAHAGVSKPVLYRYFTDKSGLWLAVGRHAAGAVVEAIAPAVSAVREERDVVAAAVDAYLAFIEADPHVYRFVVHQRGIARDTDVVADAMDTVASGLARILGDRLRALGLDSGPALPWAYGIVGYVQTTGDWWLRQQQPISRTALADYLTTFLWGGIAGIRSAADTPGGLAVSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2I9</accession>
    <name>U5W2I9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362493.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19245"/>
    <dbReference type="EnsemblBacteria" id="AGZ42121">
        <property type="protein sequence ID" value="AGZ42121"/>
        <property type="gene ID" value="AFR_19245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3909"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_6_4_11"/>
    <dbReference type="OrthoDB" id="1918349at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="16"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="119" mass="13018" checksum="51E25C3EBEC0A7F8" modified="2014-01-22" version="1">MTATLVELDEGEGAEVAVVHVRADLDATVVPTLRETLTDAVDRHRHVVVDLSAVPTLDDAGLGLLVRAHRRARRHDGMVVFAGPSRFVITVLHTMHVDGLFPIFDDCDAALEWLRAETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2L5</accession>
    <name>U5W2L5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Ribulokinase</fullName>
            <ecNumber evidence="3 4">2.7.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">araB</name>
        <name evidence="7" type="ORF">AFR_25550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + D-ribulose = ADP + D-ribulose 5-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17601"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17173"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>ATP + L-ribulose = ADP + H(+) + L-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:22072"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16880"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58226"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate degradation; L-arabinose degradation via L-ribulose; D-xylulose 5-phosphate from L-arabinose (bacterial route): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the ribulokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.16" evidence="3 4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364014.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25550"/>
    <dbReference type="EnsemblBacteria" id="AGZ43374">
        <property type="protein sequence ID" value="AGZ43374"/>
        <property type="gene ID" value="AFR_25550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5177"/>
    <dbReference type="eggNOG" id="COG1069">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_9_1_11"/>
    <dbReference type="OrthoDB" id="1619686at2"/>
    <dbReference type="UniPathway" id="UPA00145">
        <property type="reaction ID" value="UER00566"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019150">
        <property type="term" value="F:D-ribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008741">
        <property type="term" value="F:ribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019569">
        <property type="term" value="P:L-arabinose catabolic process to xylulose 5-phosphate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07781">
        <property type="entry name" value="FGGY_RBK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00520">
        <property type="entry name" value="Ribulokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005929">
        <property type="entry name" value="Ribulokinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01234">
        <property type="entry name" value="L-ribulokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0054">Arabinose catabolism</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="5">
        <location>
            <begin position="9"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="6">
        <location>
            <begin position="292"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="544" mass="57705" checksum="7B234A8AEDA74ECE" modified="2014-01-22" version="1">MPDVPSESYVIGVDFGTLSGRAVVVRVADGAELGSAVHEYPHAVLTDALPDGTPLGHDWALQVPGDYVEVLREAVPAAVRAAGVDPSLVVGIGTDFTACTMVPALADGTPLSEVEGLANRPHAYVKLWRHHAAQPHADRINQLAAERGEAWLPRYGGLISSEWEFAKALQLLEEDPELYERMDRWVEAADWIIWQLTGTYVRNACTAGYKGIRQDGHYPSPEFLAALNPAFATFVADKLDQPIGELGSAAGTLSAEAAAWTGLPEGIAVAVGNVDAHVTSAAAQALEPGQMVAIMGTSTCHVMNGAEVVEVPGMCGVVNGGIVAGLFGYEAGQSGVGDIFAHYLRYLAPEFTHEHLTELASRQRPGEHGLIALDWHSGNRSVLVDHELSGLLIGETLATRPEDVYRALIEATAFGTRTIVETFTAAGVPVSEFVAAGGLVKNSLLMQIYADVLRMPLSVIGSDQGPALGSAIHAAAAAGAYPDVRTAAAAMGRKETAVYKPDPEAAAVYDELFAEYTKLHDYFGRGGNKVMRTLRSIQRRVVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2S0</accession>
    <name>U5W2S0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19670"/>
    <dbReference type="EnsemblBacteria" id="AGZ42206">
        <property type="protein sequence ID" value="AGZ42206"/>
        <property type="gene ID" value="AFR_19670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3992"/>
    <dbReference type="eggNOG" id="COG3434">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044951_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014408">
        <property type="entry name" value="dGMP_Pdiesterase_EAL/HD-GYP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013976">
        <property type="entry name" value="HDOD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08668">
        <property type="entry name" value="HDOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003180">
        <property type="entry name" value="DiGMPpdiest_YuxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51833">
        <property type="entry name" value="HDOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EAL" evidence="1">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="HDOD" evidence="2">
        <location>
            <begin position="207"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="407" mass="43393" checksum="C49AD842B65274A7" modified="2014-01-22" version="1">MPFPLPGTQLVHVGRQPIFDVTGDVVAYELLFRGSMDAVDAGRRDTYATSQVIVNTFTEFGIDEVVGDRTCFINLTREFLTGELVLPFGPDHVVLEVLETVAVDDEVIAGVTALVGQGYRIALDDFVWGSGHEKLLPLASFVKLDLLEGDLTHLDEIVAACRAYPGIQIVAEGLETGEHLALSNRYGFELRQGYVLSRPQVLTATSLTPSRLRRLELLGALSAADTDLERILSIIAGDPALTMRVLRASNSAAVGATTKISSVRQAVVMLGIAHIRQWAMLMVVDDVAEATEVQMAEALTRARLCENVAVAFGASADAAFMAGLIGAVAGMLGLSNAAMAHQLPLSPDIAGALERGTGPIGQVLRVVNAYERGDLAEVAAAYSGDDLTGTVMDAMRWSRMAVQATQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2W4</accession>
    <name>U5W2W4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="1">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_19945</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19945"/>
    <dbReference type="EnsemblBacteria" id="AGZ42261">
        <property type="protein sequence ID" value="AGZ42261"/>
        <property type="gene ID" value="AFR_19945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4047"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_6_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665494" description="Peptidoglycan glycosyltransferase" evidence="9">
        <location>
            <begin position="33"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="11">
        <location>
            <begin position="78"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="10">
        <location>
            <begin position="427"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ42261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="714" mass="77062" checksum="CCDEFA86F6480FC4" modified="2014-01-22" version="1">MTSPTPPRGRNKFASAGSLLICGLLAGVVVAAASFPAVAMSGLAAKAGSETFAELPSELEQQTAPQVTRVFASDNKTQIAVMYDEFRSDVPLKDISVNMQNAIVSAEDHQFYKHNGVDLKGVARAFVNNKNGGGKQGASTLTMQLVRMTLAYSAKTPQEAVDATKDTPERKLSEMKYALQLEKELNKQQILERYLNIAPFGNGAYGVYAGSQVYFGKKPKDLTVPEAALLAGMVKAPTSFDPTTPSGYPQALDRRNYIIDNMRDLGYVTPEEATKAKTVKLGKTTNRPGNGCVSVAKNNWGFFCDYFYRWWLTQEAFGKTTYDRERQLKTGGYRVVTSLDVKAQSSARKEITKLIKDSDRNALLLAGVEPGSGKVRSLAANRKYKLDSATDPKNKLSSDPQKAAKGIRGTYPNTTNPIITGGGDITGYQAGSVFKMFTMVAALENGMELDYTINSQARYKSGYIIDPSSDAACPGTHFYCPSNASKSEVGNFNMWTGFGSSVNTYFVPLQEQVGAEKVVDVAKRFGVKFRASSDAKIADDEASAHQWGAFTLGVSASTPLDIANAYATLAGDGMYCVPTPIQQIYTQDGKKSDIGQPKCTRATSKDVARKALDAARCPVGDQAQLGNCSGATERNARSVVGHPIFGKTGTTDADKTAALVVGTQHLVVAGYLVNPDWAGHPDRMSHGIVNPAVYRTVADYMEGRPKEEFKKPND</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2X1</accession>
    <name>U5W2X1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell surface-attached carbohydrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_19995</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362643.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19995"/>
    <dbReference type="EnsemblBacteria" id="AGZ42271">
        <property type="protein sequence ID" value="AGZ42271"/>
        <property type="gene ID" value="AFR_19995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4057"/>
    <dbReference type="eggNOG" id="COG3507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5434">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019346_0_0_11"/>
    <dbReference type="OrthoDB" id="323324at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665642" evidence="2">
        <location>
            <begin position="34"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="3">
        <location>
            <begin position="410"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="561" mass="60408" checksum="EED0009FE6B35A04" modified="2014-01-22" version="1">MPVSRRTLLSTVAAGSAAASIPAVLTAAGPAAAASPPGDVVGKISVGYQGWFSCPGDNAPIGGWWHWSRDRFQPPSPGNTTIVSWPDLTDLTHGYPTAYPNLGNGQPARLFSSYDQQTVDTHFRWMQQYAIDTAALQRFNPFGDEGPTRDAMAVKVRGAAEATGRKFYIMYDVTGWTNMQSELKTDWTTKMSALTASPAYARQNGKPVVCIWGFGFDDPGRPFAPGPCLEVINWFKAQGCYVIGGVPTWWRQGINDSRPGFGDVYHAFNMISPWMVGRASTLADLDSYYANANVGDYADCQAHGIDYQPCVLPGDLSSGHRRHGDYYWRSLYNMIRLGSQGLYVSMFDEFNEGNQIAKTAPTAAWVPAGSGIRALDEDGTACSSDYYLRITTDGGRMLKGQLPLTATRPTPPIVTGNPDPGPDPTRDLAAGRPAAESSHTQVYGAGNAVDGNPGSYWESANNAFPQWLQVDLGSATAVARVVLKLPPGWETRTQTLQIQGSTDGGSFTTLSGSTGRTFNPATGNQVSVTFTRATVRHVRVTFTANTAWPAGQASSFEVYAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2X2</accession>
    <name>U5W2X2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25515"/>
    <dbReference type="EnsemblBacteria" id="AGZ43367">
        <property type="protein sequence ID" value="AGZ43367"/>
        <property type="gene ID" value="AFR_25515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5170"/>
    <dbReference type="HOGENOM" id="CLU_1522043_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="176" mass="17859" checksum="517578DE21C520CD" modified="2014-01-22" version="1">MHKTAAGRGKAILCTLAVAAVVGGGLWSRADRLFPDADRDEEIYTACVYAVPAGEHRALEGCGAAEERLAPGRTDDPALIQVAGRVVNVVVSSGEGLGGTPTEMVAGRARDGLVRLGYAGSVVRVARDGDPAARGTLVFGVPLTAATCLVGHLDTLSGPASWQMVGLLPGGTCLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Y6</accession>
    <name>U5W2Y6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20415"/>
    <dbReference type="EnsemblBacteria" id="AGZ42355">
        <property type="protein sequence ID" value="AGZ42355"/>
        <property type="gene ID" value="AFR_20415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4140"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_8_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="50"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="28490" checksum="CB48DEF3BEB6D5E6" modified="2014-01-22" version="1">MSPALMTTSQRRLAAAAPRPAQGFVSRRVEVAGLRLHVRSRAAATGRPWVLLHGLAVSHRYLMPTAAALEGGPVHVPDLVGFGLSVKPRPVLDVVGHSRVIAAWLDAEGIGPASVLANSFGCQVAVELAIRRPDLVAALVLVGPTVDPDAPTSAGQAGRWLLDLTREDPRQAALIAADVRDAGIRRVLTTLRLSVRHRIEQRLPLVTQPVLVLRGEHDPIVPPRWARDAGLLAPRGATGEIPGAAHNAVTTAGTEVAARALAFVGGPARPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W307</accession>
    <name>U5W307_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362683.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20195"/>
    <dbReference type="EnsemblBacteria" id="AGZ42311">
        <property type="protein sequence ID" value="AGZ42311"/>
        <property type="gene ID" value="AFR_20195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4096"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_35_0_11"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="26"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="31642" checksum="6C8F5FB3848A1B9B" modified="2014-01-22" version="1">MPVHHRYATVDGHQLFYREAGDPSAPAVVLLHGFPTSSFMFRDLIPALADRYHVIAPDHLGFGFSDAPDAADFDYSFDALAELTAKLLTGLGVTRYAIYVQDYGAPIGWRLALRPGTPVTAIITQNGNAYEAGLVDDFWKPVRAYWAHPGADTEAGVRAALDLEAIRWQYLHGVPDPTVVSPDTWTHDHALVSRPGNDVVQLRLFADYATNLALYPRLHAHLREHRPPVLAVWGRNDEIFGPAGAEAFRDDEPEAEIHLLDGGHFLLESHGAEVAALIRDFLRPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3B9</accession>
    <name>U5W3B9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative carbon monoxide dehydrogenase medium chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560041.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27210"/>
    <dbReference type="EnsemblBacteria" id="AGZ43703">
        <property type="protein sequence ID" value="AGZ43703"/>
        <property type="gene ID" value="AFR_27210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5514"/>
    <dbReference type="eggNOG" id="COG1319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058050_3_0_11"/>
    <dbReference type="OrthoDB" id="1017413at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="SSF55447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31205" checksum="F0350E9C35E8F446" modified="2014-01-22" version="1">MQVPAPFEYARATTVDEAIGLMERYGDTARLVAGGHSLLPMMKLRLANFEYLIDINDLHGELGYITTGPDEVRIGALTRHRELLESDALAAALPIFRDAERVIADPVVRNRGTLGGSLCQADPSEDLSAVCTTLGASCVIRSSSGTRTVSMEDFHRGPYETAVADGEILVEVRIPVRPRGGSAYAKVERRAGDWAVVSAGAAVWLDASGQVADARVGLAAVGPNTTGIPAISAALRGREPSEELYLEAGVIAAENCEPVTDQRGSADYKRHLAQELTVRTLRRAVARIAERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3N5</accession>
    <name>U5W3N5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27415"/>
    <dbReference type="EnsemblBacteria" id="AGZ43744">
        <property type="protein sequence ID" value="AGZ43744"/>
        <property type="gene ID" value="AFR_27415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5557"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_87_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="148"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26451" checksum="E2A9465319F72A6C" modified="2014-01-22" version="1">MSPVPSLILSIDRGESGPRDDFGTHVHPEPLLLWTWTATVLVTAAERDWLVPPGHGLWVPGDVEHSGAVLRGGAGSALVFDPATCPVAWPRPTGVSGGPLVQALITHLFETDPGDPDRVHAEALLFSRLTPLPPHDLHVTLPADPRIRVIAERLIADPADPRDLAAWADHTHAGLRTLSRLFQNETGLSFARWRTRVRVRAAIPLLAAGATVDSVARQVGYRKTSAFIAAFRRVTGQTPGTYLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3U0</accession>
    <name>U5W3U0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560025.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43687">
        <property type="protein sequence ID" value="AGZ43687"/>
        <property type="gene ID" value="AFR_27130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5498"/>
    <dbReference type="eggNOG" id="COG2315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138549_2_0_11"/>
    <dbReference type="OrthoDB" id="1817663at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007351">
        <property type="entry name" value="YjbR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038056">
        <property type="entry name" value="YjbR-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04237">
        <property type="entry name" value="YjbR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142906">
        <property type="entry name" value="SSF142906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="117" mass="13231" checksum="1BF31D5E1B3ADB44" modified="2014-01-22" version="1">MLAEVRRICLGLPGVTERLSHGAPTWFVNGQRTFVTLWEHGHHDNEFPHLWCAAPAGAQEELILADPARFFRPPYVGHRGWIGVRLDGDVGWDEIGELCQDAYDTVSRPKKSTSRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W464</accession>
    <name>U5W464_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferredoxin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560149.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27755"/>
    <dbReference type="EnsemblBacteria" id="AGZ43812">
        <property type="protein sequence ID" value="AGZ43812"/>
        <property type="gene ID" value="AFR_27755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5625"/>
    <dbReference type="eggNOG" id="COG0446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_4_0_11"/>
    <dbReference type="OrthoDB" id="1149616at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028202">
        <property type="entry name" value="Reductase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14759">
        <property type="entry name" value="Reductase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="7"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Reductase_C" evidence="2">
        <location>
            <begin position="325"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="415" mass="43977" checksum="E18D8D47298C16F5" modified="2014-01-22" version="1">MTGDRTFVIAGAGLAGAKAAETLRAEGFTGRIALIGTETEIPYERPPLSKGLLLGTAQRSEAFVHDEAWYASNNVELRTGTTVGAIDAEKQRVRLSDGDDLAYDKLLLATGASPRPLGVPGAGLGKVFSLRTLADSDRISETVRSDTRLVVVGSGWIGLEVAAAARARGATVTVVSPAALPLQKVLGDRLARVFLGLHQEHGVAFRFGTQVSEVREARGDTDVSQVVLSDGSVIDTDAVLVAIGVTPNTGLADQARLRVDNGVLVDGQLRTSDPDIFAAGDLANIDHPLLGARVRVEHWANALHTGPVAAKAMLGQDATWDRLPYFFTDQYDLGMEYAGWVPPGSTTEVVVRGDEEGREFIAFWTVGGRVAAGMNVNVWDVTDQIQDLVKAGLRGATVDPARLADPDVPLTDLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4D8</accession>
    <name>U5W4D8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560346.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28740"/>
    <dbReference type="EnsemblBacteria" id="AGZ44009">
        <property type="protein sequence ID" value="AGZ44009"/>
        <property type="gene ID" value="AFR_28740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5826"/>
    <dbReference type="eggNOG" id="ENOG50334H9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147936_0_0_11"/>
    <dbReference type="OrthoDB" id="1829674at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="17139" checksum="041F58DE814782A3" modified="2014-01-22" version="1">MTRPDRRVRLDDLGAWLLKGNADQSDLAGRFARDPRVTRWCLQPSYRLELMRAGQPVLFWASGSRRRDTAYGIWGRGELAGTPERDGDAGWWVPLDLVIAGPADRLPREELRADPRLSELEVLRQPQAANPSFVTVAQYAVIRCLSGDPAGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4K7</accession>
    <name>U5W4K7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560475.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29385"/>
    <dbReference type="EnsemblBacteria" id="AGZ44138">
        <property type="protein sequence ID" value="AGZ44138"/>
        <property type="gene ID" value="AFR_29385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5956"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034451_4_0_11"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="159"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="307"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="384" mass="41426" checksum="AB2260E41E46408B" modified="2014-01-22" version="1">MVAIPETREFVGRRPETDEALAVLPATSQAALRQFSIERTVGYGVDYADAVELRGRVVDGQDWQQAATDLARTCLSRAESAPAAFVPTRAGYLRRASALLRMSQMMMLSDTRERREIYAGAAHLYNQAAELLGDRRRVSIRTDREPLIGWLFSAARPAGASVVVIGGVEGHAMDFDSLGVAFAARGVDALVLDGPGQGESRFAHHHYLSAQWLDAYRHAIDYLDRRAPGRPIGFAGNSMGGSFAMTVAAADVRISACCDNGGILAPWLVPPSAGAFHSKMLAFCGTDDPEQAMQAWKTVAPGTTGPNAGYPLLIVHGGRDPLVSTEQARLVLDSVPTADKQMVVFTDGEHCIYNHKTDRDVLITDWMTARLTGTARPTTSNPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4V0</accession>
    <name>U5W4V0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29835</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560565.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29835"/>
    <dbReference type="EnsemblBacteria" id="AGZ44228">
        <property type="protein sequence ID" value="AGZ44228"/>
        <property type="gene ID" value="AFR_29835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6043"/>
    <dbReference type="eggNOG" id="COG1877">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101802_0_0_11"/>
    <dbReference type="OrthoDB" id="1808149at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="158" mass="17169" checksum="BA868DF3696F4600" modified="2014-01-22" version="1">MSTLIFLDVDGPLIPFGPRPDGHRGPPPHATADHPLLHRLNPADGPRLLALGAELVWATTWMREANEIIAPLLGLPQLPVADWPDDEPPAGLHWKTAGLVAWAAGRSFTWIDDEITDTDRRWVAAHHPGPALLHRVDPRAGLTDADFTVLRAGARLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W568</accession>
    <name>U5W568_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363710.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43120">
        <property type="protein sequence ID" value="AGZ43120"/>
        <property type="gene ID" value="AFR_24270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4916"/>
    <dbReference type="eggNOG" id="ENOG5032XAF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116748_0_0_11"/>
    <dbReference type="OrthoDB" id="1705442at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046080">
        <property type="entry name" value="DUF6098"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19593">
        <property type="entry name" value="DUF6098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="17743" checksum="1E93E167474D8410" modified="2014-01-22" version="1">MRHFVSEKLPTLAGLDDLTDLVTKSAEEDQLFVRWSAGPDADLRRDGTSRDSLTGVPLPGLSANPLAVEPWWGDRSPRLWVARRLYDYRHLRYLRGPEVRPWVLVGEQCGRGPDNEPLVRVRRPVAWIAEAALIEGEQLIEAQHSAECGPLDRRSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5B9</accession>
    <name>U5W5B9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30765"/>
    <dbReference type="EnsemblBacteria" id="AGZ44413">
        <property type="protein sequence ID" value="AGZ44413"/>
        <property type="gene ID" value="AFR_30765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6228"/>
    <dbReference type="eggNOG" id="COG4291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081563_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="24809" checksum="E7D04815F8809F88" modified="2014-01-22" version="1">MGIAKDLEQLPFWRQNSRGEHRWPSALAIAAMIGLQLALPDRYSVVAWWVLPGFELVIIVLLFGMDPGRVKRRTPVLRRLSLSLIAVASLGTAWAVVRLVQDIATGHGTGSAGQLLSTGGMIWLINVLTFAVWYWELDRGGPAERAAGSDPYPDFLFPPMTSPGMVPTDWRPQFADYFYLAFTNATAFSPTDTLPLSRWAKIAMLLQSAISLVTAALVVAKAVNALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5C0</accession>
    <name>U5W5C0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_29730</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyltransferase 87 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29730"/>
    <dbReference type="EnsemblBacteria" id="AGZ44207">
        <property type="protein sequence ID" value="AGZ44207"/>
        <property type="gene ID" value="AFR_29730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6023"/>
    <dbReference type="eggNOG" id="COG5650">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034641_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018584">
        <property type="entry name" value="GT87"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09594">
        <property type="entry name" value="GT87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="407" mass="43391" checksum="1722589F9ECC3620" modified="2014-01-22" version="1">MSQAVHPVRQPRAMRGLGPILAVIGAAAVLAGATAWAEIPAKLSVHGEFVDLSVYRYGGRLILDGLPLYGSRDPATGLAFTYPPFAAVALVALEPVPFWLATAWWTAASVGAMAGTILVAGHALGRPVPHRLVALLTVGALALEPVWQNLTFGQINLLLMLAVLIDLVHPDRRWSGVLVGIAAGVKLTPLVFVVLLVLIHRRAAAGRALLAFFATIAIGFAVIPGSAKAYWTDNLIEAGRVGPPELAHNQSVFGALTRLLDVPAPTLLWLAVAGPLALAIVVVGALWWRRGDRVLGAGFAALAMLFASPVSWSHHWVWAAPIGLALWSYNWWASIVWTAVFVARPFVWPPWGQSREYRWGPIEHIIGNAYLLAALALCVWAAVRLSAGGMAVPAADGHASLDQKAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W672</accession>
    <name>U5W672_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PG_binding_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364261.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43500">
        <property type="protein sequence ID" value="AGZ43500"/>
        <property type="gene ID" value="AFR_26190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5308"/>
    <dbReference type="eggNOG" id="COG3342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068244_0_1_11"/>
    <dbReference type="OrthoDB" id="1771686at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010430">
        <property type="entry name" value="DUF1028"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014927">
        <property type="entry name" value="PG-bd_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06267">
        <property type="entry name" value="DUF1028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08823">
        <property type="entry name" value="PG_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665739" description="PG_binding_2 domain-containing protein" evidence="1">
        <location>
            <begin position="35"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_2" evidence="2">
        <location>
            <begin position="209"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="28468" checksum="50BB840676AAFDB2" modified="2014-01-22" version="1">MTFSIVARSADGCALGVAVASKFLGVGAAVPAALAEVGAVATQSYANLAYRPQALALLGTGVPATETVAGLIAADDGPVDHRQVGVVGTSGPGATFTGPACHPWAGGAAGDGFAIQGNMLAGPQVVDRMRDEWLAGAEITRLAYRLLAALRAGDEAGGDRRGRQSAALLVVAKGQGYGGTSDVLVDLRVDDHTAPVTELGRLLEMHSLYFERPDPATLLELSGPVGDEVRALLAAAGHQAGPDLADLDEALASWAGIANLEERIVAGHIDPLVLDQLRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6A4</accession>
    <name>U5W6A4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_26085</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM175 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364224.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43481">
        <property type="protein sequence ID" value="AGZ43481"/>
        <property type="gene ID" value="AFR_26085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5287"/>
    <dbReference type="eggNOG" id="COG3548">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090238_3_0_11"/>
    <dbReference type="OrthoDB" id="1399320at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010617">
        <property type="entry name" value="TMEM175"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06736">
        <property type="entry name" value="TMEM175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ43481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="24614" checksum="300908DF715F5CE3" modified="2014-01-22" version="1">MTAENTDVEAGRTPERVGFFTDAVFAIAMTLLVIEIPRPEEAPEFSVGDGVSKTQAAGHLWHFLTGQTGSFVAYLLAFFMLWTAWRQHHTLFDRIGRLSPGLVAWHFPLLLLIGFLPYPATVYGHHTANPAAALLFVLTVGGLLICRAGVQSQALRDDLLHAHVDASEVAAGARASWIVAAYWLVTIVLCWWTPWVIIAWTLTPVLGAILNRGSRRRRTSIGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W725</accession>
    <name>U5W725_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ThuA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560074.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27375"/>
    <dbReference type="EnsemblBacteria" id="AGZ43736">
        <property type="protein sequence ID" value="AGZ43736"/>
        <property type="gene ID" value="AFR_27375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5549"/>
    <dbReference type="eggNOG" id="COG4813">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084426_0_0_11"/>
    <dbReference type="OrthoDB" id="1452706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029010">
        <property type="entry name" value="ThuA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009381">
        <property type="entry name" value="Trehalose_catabolism_ThuA_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06283">
        <property type="entry name" value="ThuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030013">
        <property type="entry name" value="ThuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThuA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="254" mass="28952" checksum="C693751F34CEA0BE" modified="2014-01-22" version="1">MPEIRVTVWGENRHEQVEEHVAKIYPDGMHETIAAGIRENLGEAATVRTVTLDDPEHGLTEEVLAQTDVLTWWGHAAHGEVADEVVERVHRHVLSGMGLVVLHSGHWSKIFGKLMGTTCTLRWRNEHDRELLWTVDPSHPIAQGVPHPIVVDEDEMYGEYFDIPAPDELIFISSFSGGEVFRSGCTWRRGYGKIFYFKPGDQDYPVYHHKDIRRVIANGVQWARSDRPARAVPELLRYDTGEFFEGRSYSGPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7A8</accession>
    <name>U5W7A8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28095"/>
    <dbReference type="EnsemblBacteria" id="AGZ43880">
        <property type="protein sequence ID" value="AGZ43880"/>
        <property type="gene ID" value="AFR_28095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5695"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064093_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="35321" checksum="D5FC2CEBABC48B26" modified="2014-01-22" version="1">MIRAMSVASAQPRPVFDLPAAYPEMGRVREALQAADWAGARAVIEAAPDPAARTMLVGFAADTDGIELLLREALDRDPGDALAGAVLGGHLVRTGWDVRTGARAGDVSAEQFRVFHDYLRRAEQVLIDAAARNPAEVSIWCQRLTSARGLQLGLSEVRRRYDRLAAYDPHHLPAQSQLLQSLCPKWSGTWEQVHGFAYERMLAAPKGAHNAVLVVTGHLERWLDADGGPAGAHYLGSPFARNQIYEAAARSVWDPGFRHTYGWVWVRSSFAMIFSLLGDEPAAAAQFAALGHLASEDPWHYLGDHVQVVTDRRARAYAKAGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7H8</accession>
    <name>U5W7H8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34030"/>
    <dbReference type="EnsemblBacteria" id="AGZ45064">
        <property type="protein sequence ID" value="AGZ45064"/>
        <property type="gene ID" value="AFR_34030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6888"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2068072_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="51"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12742" checksum="CBCF9AE55883BFDB" modified="2014-01-22" version="1">MFHPAATGRYPGKAPLPARPVPVLADPWPGVPVRGRNAAGRADACWLPIAPKLTPHGLRHTYKTIMVELGTPATLMDDQMGHEDGSVQARYAHITSGMTERLLGGLTELWLAALTARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7R1</accession>
    <name>U5W7R1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34505"/>
    <dbReference type="EnsemblBacteria" id="AGZ45159">
        <property type="protein sequence ID" value="AGZ45159"/>
        <property type="gene ID" value="AFR_34505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6980"/>
    <dbReference type="eggNOG" id="ENOG5032319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840812_0_0_11"/>
    <dbReference type="OrthoDB" id="2182960at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="15180" checksum="830A9BC40E0C82F1" modified="2014-01-22" version="1">MGYASGRAGGYLYGDALDGDIDLTDPLMRWQRLEAFAVMFRQAVLPWFDEAGDPERIASSRAGDCTNRPSALVEWLASRDQEARIGTYVHRYLARTPGAQQQYETGRAKGEAGLSHAELQGGDMAEALGWSVAKLTPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7S5</accession>
    <name>U5W7S5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MarR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560343.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28725"/>
    <dbReference type="EnsemblBacteria" id="AGZ44006">
        <property type="protein sequence ID" value="AGZ44006"/>
        <property type="gene ID" value="AFR_28725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5823"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142189_0_3_11"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027395">
        <property type="entry name" value="WH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13601">
        <property type="entry name" value="HTH_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_34" evidence="1">
        <location>
            <begin position="14"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="10761" checksum="4CF447A349CB0112" modified="2014-01-22" version="1">MTAGLDPIIHPTHRLRICAMLVAGKALELSVIKEHLDLSPSALSKQVAALVDAGYVNQERLGSDTRRVWLSMTRQGMKAYQGHVAALREIVNTTGVPSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Z6</accession>
    <name>U5W7Z6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560378.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28900"/>
    <dbReference type="EnsemblBacteria" id="AGZ44041">
        <property type="protein sequence ID" value="AGZ44041"/>
        <property type="gene ID" value="AFR_28900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5858"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079903_0_0_11"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="252" mass="27824" checksum="D4FC1A9871B3CC7C" modified="2014-01-22" version="1">MTWSTRELAELAGTTVNTIRHYHRLGLLDDPDRRYNGYKQYDVSHLVCLLRIRRLVELGVPLSEVGVARSAGDVPPEVLRQVDAQLAAEIERLSRARADIAVILRDGAPADTPAGFESVAPRLSTADASLIHVYSRLYDDDAMKDLVRMVETDTDPVNAEMNALPVDADEATRQSVAERLAPVIAQNLLDYPWLSDPAAHLSNSEQVTQKTFVDAVSTLYNAAQLDVLTRASLLAHEQVRRTRAGEQDADVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W807</accession>
    <name>U5W807_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GAF-sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28950"/>
    <dbReference type="EnsemblBacteria" id="AGZ44051">
        <property type="protein sequence ID" value="AGZ44051"/>
        <property type="gene ID" value="AFR_28950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5866"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_339712_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="35"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="201"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="353"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="836" mass="86733" checksum="557936C328C5714D" modified="2014-01-22" version="1">MGDGAPVTVPAGDDATDGQLDALRRVAELIARGAGRTAVLDAVVAEAYSLFPVEFTAILRYEPGGAASIVAVHHGPPGLLVGERAPYIPQGLVLRVFGSGRPARVEAYGDLPDRGLARMRELGISAGAAAPIRVDGRLWGVLTAMTQSGPVVPGLEHRLVEFAEVAAVAIASAQARAGLRLIAPEQAALRRVAEAMAHGGAEQQLFDTVTEEVSALLGAEGATLVRFSGRRASVIATVGPAVEIAEDAVAAILRTRLPVRLDTSGGPGSEQAGAGDGSSVAVPIMVKDRLWGMLGVSTTGHQLPADTERRLGQFTELVATAVATSEAWARAEQLAGHQSALRRIAELAARDAPGEQVLEAITVQAAKLTGVQFTAILRFEPDGSREIMALSGAPAGLRPGMRTPANDGATGRVWRTNRTARVEGLDDVGGPWAQIARDAGFSAGTAVPIRMHSRLWGTLVAVGRTEPLPASVEDDLTSFAELAGTAISATQARNDLRALADEQAALRRVAELVAIGTALDDVFQAVAGEAAKLLGSDHARLQRYERDGTTVTVAAYDAGAETTLRADQQVTVPVVVEGRVWGDLTAGTTGRALPESMEERLRQFAGLAAAAIANAENKAHLTASRARVVATADETRRRLQRDVHDGAQQRLVHTIIALKLARAAIARGEMPGAEVSEALQHAERANAELRDLVHGILPAGLAQGGLRTGLESLIDDLSIPVDLRFTAPRLPAQTEITVYFFVAEALTNVVKHARASRAGVTVQVDGTLLAAEVRDDGIGGADPSGGSGLTGLSDRIQAADGTLALISPAGGGTTVRAVLPLPGTDVKSLPGYASAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W824</accession>
    <name>U5W824_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560457.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29295"/>
    <dbReference type="EnsemblBacteria" id="AGZ44120">
        <property type="protein sequence ID" value="AGZ44120"/>
        <property type="gene ID" value="AFR_29295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5936"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_4_11"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="468" mass="49780" checksum="EBE370676F8665AF" modified="2014-01-22" version="1">MDSEIYFSDVTKLAHLIRTRELSPVEVMRAHLDRIEATNPQVNAVVSLADGALDAARRAEAAVTDGGDLGPLHGVPFTVKDSIDTAGVLTQRGSPIFKGRVPDTDATSVARVKQAGGILIAKTNLPEFSYSTESDNLLSGRTNNPWNLDRTPGGSSGGESAAIAAGMSPFGLGSDLAISVRGPAAHTGIIALKATHGRVPATGMWPRVPRRDWHIGPMARTVRDIALAYSILQGPDGADGYSIIPRGFDAGVGSAPNRPIRIGWLVEPGLGPIHPEVAATVEAAAAAFQGMGIAIEQVRIPALERDLALNVYNKQHVMELKPAFAEVTRGHEDEIGQITRTTLDTPDTSVADYVGAEQWIEQLRDGFAAYFQKFDALLLPATPIPAHAHGLQEFVINGETVPFIYMQNATVPFNMTGLPALSMRFGTSSEGLPIGVQIAAPWFAESTILHLASLLESVSPVRNLHPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W832</accession>
    <name>U5W832_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBM6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_29075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29075"/>
    <dbReference type="EnsemblBacteria" id="AGZ44076">
        <property type="protein sequence ID" value="AGZ44076"/>
        <property type="gene ID" value="AFR_29075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5891"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043495_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006584">
        <property type="entry name" value="Cellulose-bd_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03422">
        <property type="entry name" value="CBM_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00606">
        <property type="entry name" value="CBD_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666190" description="CBM6 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="3">
        <location>
            <begin position="25"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="453" mass="46240" checksum="3BA1D1BE6D501CB5" modified="2014-01-22" version="1">MAITGALVTGGLAAASGSASAATTVTVQAESYAAQSGAQLETTADTGGGQNVGYLANGDWLRYDNVDLGASGAITLGARVASDSGTTGSIQVRTGSATGTVLASIPVTSTGGWQTWRTSTAVANTHPTGAQTVFLTLASSQAGNFANLNWFSVTSGGGTTPTTPAPTGTSGPGWPTVNAAQQAADTAAFFALTPRPITGPVVRVPEFNAGCTVSHHLNDDPIIFPGMAGASHAHTFFGNTSTNANSTNQSLLGHTVTTCAPAEDLSAYWIPSLLQNGTVIDPAGVTVYYGSRLQDPSKTQPFPPGFRMIAGDAKLQVPSSGNQFWCAGPGGQTGRSTDGNWPICAQTANLTYQLVFPDCWDGVHLDSPNHKDHVGPTGPDGTCASGRFPVTIPSLSFVIDYPVHGTPAGFTLASGMASSMHGDFMNAWQPAAQAARVRNCIDQSVKCNSAGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W887</accession>
    <name>U5W887_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5-oxoprolinase subunit A</fullName>
            <shortName evidence="1">5-OPase subunit A</shortName>
            <ecNumber evidence="1">3.5.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-oxoprolinase (ATP-hydrolyzing) subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pxpA</name>
        <name evidence="2" type="ORF">AFR_34830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-oxo-L-proline + ATP + 2 H2O = ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10348"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58402"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.5.2.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex composed of PxpA, PxpB and PxpC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LamB/PxpA family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561558.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34830"/>
    <dbReference type="EnsemblBacteria" id="AGZ45222">
        <property type="protein sequence ID" value="AGZ45222"/>
        <property type="gene ID" value="AFR_34830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7047"/>
    <dbReference type="eggNOG" id="COG1540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069535_0_0_11"/>
    <dbReference type="OrthoDB" id="918580at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00691">
        <property type="entry name" value="PxpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005501">
        <property type="entry name" value="LamB/YcsF/PxpA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30292">
        <property type="entry name" value="PTHR30292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03746">
        <property type="entry name" value="LamB_YcsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="26639" checksum="DBD0515736BEA919" modified="2014-01-22" version="1">MDLNSDLGEGFGTWRLGDDTAMLDVVSSANIACGFHAGDPRTLLATCERAVATGVVIGAQVGYRDLAGFGRRFIDYETPDLVADVIYQLGALDGLARAAGDRVLYVKPHGALYNTIVHHEQQAAAVVDAVRRYDPDLPVLGLPGSVFLHLADEAGLRTVREAFADRGYHPDGTLVSRRDPGALLHDPAEVADRMLRLVTDRTLVAVDGTVIKVEADSICVHGDSPGAVGMARAVHAKLTAAGIPITSFAGSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Q0</accession>
    <name>U5W8Q0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36460"/>
    <dbReference type="EnsemblBacteria" id="AGZ45548">
        <property type="protein sequence ID" value="AGZ45548"/>
        <property type="gene ID" value="AFR_36460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7378"/>
    <dbReference type="HOGENOM" id="CLU_1691713_0_0_11"/>
    <dbReference type="OrthoDB" id="2156587at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="16806" checksum="E0B097937DDC4B92" modified="2014-01-22" version="1">MFSGKRQVYLLPRNSEATFALDKAGRMTLSEDFGDRALFVLTPVRDDKFWIRTAKVRSGGEALCVTVKGKSVVTAGCDTTTESQLFRFRRTGESNGKPTYTIRTGKDIYLVQDPDGKLGNTGTGVAAVQIGEGTPDIDTPFVLPDRGKATLPALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8X7</accession>
    <name>U5W8X7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561808.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36080"/>
    <dbReference type="EnsemblBacteria" id="AGZ45472">
        <property type="protein sequence ID" value="AGZ45472"/>
        <property type="gene ID" value="AFR_36080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7302"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119559_0_0_11"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="174" mass="18802" checksum="4406D697CCEE24EA" modified="2014-01-22" version="1">MDPTSEPLEHRVSALEERLQELITSITPPSPAAQSKPAARPDIFWALDGLRDRVDGSGAVLYTGTVDLPTGEHFDWQYGATVDDLFADDWSEWAATLSALGHPVRLLLLRRVLAGVRTAAELSADEALGTTGQLYHHLRQLVNAGWLRSAARGQYAVPGDRVVPLLVVLSGAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Y5</accession>
    <name>U5W8Y5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="11">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="11">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">gatB</name>
        <name evidence="13" type="ORF">AFR_36970</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561984.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36970"/>
    <dbReference type="EnsemblBacteria" id="AGZ45648">
        <property type="protein sequence ID" value="AGZ45648"/>
        <property type="gene ID" value="AFR_36970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7479"/>
    <dbReference type="eggNOG" id="COG0064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_0_0_11"/>
    <dbReference type="OrthoDB" id="497127at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatB_Yqey" evidence="12">
        <location>
            <begin position="349"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ45648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="497" mass="53401" checksum="6A1B09932773D90D" modified="2014-01-22" version="1">MSTTVLPSYEDAIARYEPVIGLETHVELGTNTKMFCGCPTVFGGEPNTQVCPVCLGLPGALPVANRAAIEATIRIGLALNCNIAEWCRMARKNYFYPDMPKNFQTSQYDEPLCVDGYVDVDIDGETFRVEIERVHLEEDTGKSLHVGGSTGRIHGATESLVDYNRAGIPLVEIVTKPVPGLGARAPEVAKAYVTELRDIIRSLNVSDVRMEQGSMRCDVNTSLNLPGDEWGTRTETKNVNSLRSVERAVRSEIIRQAMLLNEGTRIFQETRHFQETTGDTRPGRSKETATDYRYFPEPDLVPIAPDTAWVAELKAALPPVPSVKRARLAEEWGISKDEMQSVVNAGATELIEQTIAAGATPAGARKWWMGELARRANETGVELADVGATPAQVAQLQGLVDEGKLNDKLARQVLEGVVSGEGDPAAVIAARGLGVVSDTGALTAAVDEAIAANPDIAAKVRDGKVAAAGALVGAVMKTTRGQADAKTVRELILARLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W916</accession>
    <name>U5W916_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arginase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37145"/>
    <dbReference type="EnsemblBacteria" id="AGZ45683">
        <property type="protein sequence ID" value="AGZ45683"/>
        <property type="gene ID" value="AFR_37145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7515"/>
    <dbReference type="eggNOG" id="COG0010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_363574_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782">
        <property type="entry name" value="PTHR43782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="382"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="565"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="627"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="639"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="692"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="809"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="857"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="949" mass="99310" checksum="B76429835702BC3B" modified="2014-01-22" version="1">MRRIAVLDAPSNLGLRPPTPTSVPGCAKAPGALRDHGLLRRLDARDAGCLTPPRYDPGDWRPGDGVSHASEISAYSRKLADRIGAILDQGEFPVILGGDCSIVLGSALAMHRLGEAVGGRIGLVFVDGHSDFRHPGNASYVGAAAGEDLALVTGRGQHDLAAMEGRRPYYRDIDVVVMGIRAQDEYRLDLQAAGIVTRPVPTLRAEGAARSAQWARDQLVDCAGYWVHVDVDVLDPAVMPAVDAPEPGGIAFPELELLLSGLVESPHCLGVEITVFDPDYDPDGAYAEEITSTIVAGLKAAHTVDARPDLVAARRDLAGPGRLGPGRLSTPPAAAVEERRAAAARDDIRLAAAGMSSGPGEVALPAATLQAVENAPFPAAEFFPGATASDPADSDPAATASDPADSEPVAGVSGRADSEPVGGVSDRADSESESEHAAADLDPQAADTPEPLADSDHPALEDPDAALTVPDAALAVPDAALAVPDAALAVPDAALAVPDAALAVPDAALAVPDAALAVPDADLDDPDATVDDLEEADEQAKALAADAGPIDSEPVDFHEQPSNELAEHDAADHEPPGAEPVGHQPADAGPDDHDLTDQTADDRELPDQVLVALDRLAHQYPVHPSVVGQYPVHPSPADHDHSGPADHYYDHVHPGWADQDAVHSGLADQDPDHPGPADQVLGGGELVNHGLEDHGSVVHEPADDEPANSESADDELLDHTSVDHETDGAERADVESADHEQADTNLGHPVEADHDPAGHQPVSHEFDDHEFEDHEFEGHGSADHATVAIEPDDPQPADIEPTDIEPADQTPADQERADQERADQEPVGQELAEGTPTGQERADQESAGQEPADQEPADQEAVGRSERDRWSERNLVTADHAEHDGYDPEQDDSVVRTAPRPLGSAPLLDSKPLPATMPGMLRPRAVEEFSLPAQRPAFEIGPEQPADLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W994</accession>
    <name>U5W994_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560849.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31280"/>
    <dbReference type="EnsemblBacteria" id="AGZ44516">
        <property type="protein sequence ID" value="AGZ44516"/>
        <property type="gene ID" value="AFR_31280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6331"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_1_11"/>
    <dbReference type="OrthoDB" id="1497607at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="178" mass="20198" checksum="ED6CF71D56CD3980" modified="2014-01-22" version="1">MTELTTERLILRRWRPADLDALAEMNADPEVMRYIRDGTVQDRGESEAFLARAEANFEKNGYGLFAAELRDTGDLAGWVGMAVPEFLPEVLPAVEIGWRLRRAYWGSGLATEGAREAMRFGFADCGLDRLVSIRHADNGRSGRVMEKLGMSFDRRTTVPATKQPVDVYEISRDEWLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9R0</accession>
    <name>U5W9R0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sulfonate/nitrate/taurine transport system ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_38445</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562278.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38445"/>
    <dbReference type="EnsemblBacteria" id="AGZ45943">
        <property type="protein sequence ID" value="AGZ45943"/>
        <property type="gene ID" value="AFR_38445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7777"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="17"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="53"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="28589" checksum="C7C435B322100953" modified="2014-01-22" version="1">MSILEAADTRTDRDAVVSLDGVSKQYGSGSNALLALDKVSLTVAKGEFVCLLGASGCGKSTLLSLVAGLDEISGGTLDIGGRHVSLMFQEAALFPWLSVAGNVELPMRLRGVGKTERRERAQELLEVVRLKDFGHKRPHELSGGMRQRVALARSLAQNADILLMDEPFGALDAMTRDILHDELERIWREQQLTVLFVTHNVREAVRLGDRVILLSSRPGRVIEDFPITHQRPRRIDSPEVSGQAAEITDRLRAEVARHAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA61</accession>
    <name>U5WA61_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ricin B-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39020"/>
    <dbReference type="EnsemblBacteria" id="AGZ46058">
        <property type="protein sequence ID" value="AGZ46058"/>
        <property type="gene ID" value="AFR_39020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7897"/>
    <dbReference type="eggNOG" id="COG3828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032985_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010496">
        <property type="entry name" value="3-keto-disaccharide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029010">
        <property type="entry name" value="ThuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06439">
        <property type="entry name" value="DUF1080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06283">
        <property type="entry name" value="ThuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ricin B-type lectin" evidence="1">
        <location>
            <begin position="418"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="552" mass="58590" checksum="E4C1D5BB7C9461B9" modified="2014-01-22" version="1">MLLPAPAVVADDPYDVLVFSRTAGFRHDAIAVGTQAIRDLGAANSFTVTATEDPAAFTTAGLAKYEAVVFLNTTGDVLDNTQQTAFEGYIRNGGGYVGVHAAADTEYDWPFYGNLVGAYFASHPAIQPATVVTEDRAHAATAHLPQRWNRTDEWYNYRTNARSTAHVLATLDESSYSGGGMGADHPHTWCKTYEGGRSFYTGGGHTQESYADPAFRAHLLGGIRYAAKASNADCRPETGYTPLYNGSTAGWSQAGPGSFTNSDATLTTVGGLGMLWYSAKQFTNYSLKLDWKLAGDDNSGVFIGFPPSTDPWSAVNNGYEIQIDATDAVDRTTGAVYTFKSADVAARDAALNPAGEWNTYELLVEGERLQVFLNGTKINDFTNTDPVRSLAGHIGLQNHGDGDDASFRNVRIKELGGTPPPVPGTGPIVGLANKCLDIANSSTADGTKIQLYTCNGTNAQKWTVTPNSTVKALNKCLDVAGAGTANGTKVQLYTCNGSGAQNWSAGTGQTLRNPASGKCLDVSQNSSANGQQIHIWDCIAGAANQKWVLPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAD8</accession>
    <name>U5WAD8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">UMP pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_39495</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562487.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39495"/>
    <dbReference type="EnsemblBacteria" id="AGZ46153">
        <property type="protein sequence ID" value="AGZ46153"/>
        <property type="gene ID" value="AFR_39495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7990"/>
    <dbReference type="eggNOG" id="COG0035">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067096_2_3_11"/>
    <dbReference type="OrthoDB" id="1581906at2"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="Ura_phspho_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ46153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="22351" checksum="0283622A91C86950" modified="2014-01-22" version="1">MDVLVVDHPLAQSRLTVMRDAETDSGAFRAALHELTTMVVYEAARTFAVEQFPITTPVAPTEGTRLANPPLIVPVLRAGLGMSDAALALLPESSMGFVGLARDEATYEPRAYMESLPGDLSGLPVLVLDPMLATGGSLVHCCQLLVDRGCTDVIICCVLAAPEGIERLRDSGLPLRLVTASIDEGLNEKMYIVPGLGDAGDRQFGGMPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAP4</accession>
    <name>U5WAP4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562542.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39775"/>
    <dbReference type="EnsemblBacteria" id="AGZ46209">
        <property type="protein sequence ID" value="AGZ46209"/>
        <property type="gene ID" value="AFR_39775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8047"/>
    <dbReference type="eggNOG" id="ENOG5032T0S">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086041_2_0_11"/>
    <dbReference type="OrthoDB" id="1952768at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025492">
        <property type="entry name" value="DUF4383"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14325">
        <property type="entry name" value="DUF4383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="16463" checksum="11CFA3D98E49B2F4" modified="2014-01-22" version="1">MAHYPLNHHLRQPYRWLTAASGLYLTLFGILGIAFTWGDPFFHRGSDWVLGLRTNPAAAWLSTVLGLVVLAAALVGGNLHHRVNLVLGWGILGVAMTLMAMIQTDANVLNVSMVNIIVLTVLGLFVLVGGLYGKVGSDEESRHEREAGLLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB32</accession>
    <name>U5WB32_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar motor switch protein FliM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fliM</name>
        <name evidence="12" type="ORF">AFR_40500</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40500"/>
    <dbReference type="EnsemblBacteria" id="AGZ46354">
        <property type="protein sequence ID" value="AGZ46354"/>
        <property type="gene ID" value="AFR_40500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8197"/>
    <dbReference type="eggNOG" id="COG1868">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052646_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17908">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001689">
        <property type="entry name" value="Flag_FliM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02154">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002888">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00955">
        <property type="entry name" value="FLGMOTORFLIM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="12" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="12" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliMN_C" evidence="11">
        <location>
            <begin position="236"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ46354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="34069" checksum="5473C8DF22751D75" modified="2014-01-22" version="1">MTTSPTRSPGKMSRRGNTGPTAYDFRRPIKLSREHIRTLQIAFETYARSCGTLFTTRLRVVSSVSLVSIEQLNYDEYVASLSNPTVLAVVSIDPLPGTVLVEMASSAVMTAIDHMLGGPGGAQPERPLTEVEMPLLRGLLERMLGELRYGFETLVDISPKLKDIEYNAQFLRAHQPGDAVVIASFEMKIGTEECVASICLPFSTILPVLEKQETIELTAAERMVRDTSFRNLTAGLSAAPIDVAVRFQPIRMRTDDIVDLRPGDVVPLGHPTSVPLEVTVNETVFAHAVPGNQGARLACLVVPSPKEEERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBC3</accession>
    <name>U5WBC3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase</fullName>
            <shortName evidence="7">MECDP-synthase</shortName>
            <shortName evidence="7">MECPP-synthase</shortName>
            <shortName evidence="7">MECPS</shortName>
            <ecNumber evidence="3 7 8">4.6.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ispF</name>
        <name evidence="10" type="ORF">AFR_41270</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7 8">
            <text>4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP</text>
            <dbReference type="Rhea" id="RHEA:23864"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="4.6.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the IspF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.12" evidence="3 7 8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41270"/>
    <dbReference type="EnsemblBacteria" id="AGZ46508">
        <property type="protein sequence ID" value="AGZ46508"/>
        <property type="gene ID" value="AFR_41270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8352"/>
    <dbReference type="eggNOG" id="COG0245">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084630_1_0_11"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00095"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008685">
        <property type="term" value="F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00554">
        <property type="entry name" value="MECDP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00107">
        <property type="entry name" value="IspF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003526">
        <property type="entry name" value="MECDP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020555">
        <property type="entry name" value="MECDP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036571">
        <property type="entry name" value="MECDP_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43181">
        <property type="entry name" value="PTHR43181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02542">
        <property type="entry name" value="YgbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69765">
        <property type="entry name" value="SSF69765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00151">
        <property type="entry name" value="ispF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01350">
        <property type="entry name" value="ISPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YgbB" evidence="9">
        <location>
            <begin position="7"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="7">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="7">
        <location>
            <begin position="39"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="7">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="7">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ46508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="16540" checksum="5DE6E103315ACFC4" modified="2014-01-22" version="1">MSTLSQRVGIGTDVHAFDPSRVCWVAGLMWPGESGLAGHSDADVAAHAACDAMLSAAGLGDLGSNFGTAEPEWAGAAGVTLLAETVRRLREHGFAVGNVSIQVIGNRPKIGKRREEAQQVLSEAVGAPVTVAGTTTDGLGLTGRGEGLAAVATALIYQEGSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBI8</accession>
    <name>U5WBI8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561652.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35300"/>
    <dbReference type="EnsemblBacteria" id="AGZ45316">
        <property type="protein sequence ID" value="AGZ45316"/>
        <property type="gene ID" value="AFR_35300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7142"/>
    <dbReference type="eggNOG" id="ENOG5032TEI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109360_0_0_11"/>
    <dbReference type="OrthoDB" id="1905807at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021443">
        <property type="entry name" value="DUF3093"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11292">
        <property type="entry name" value="DUF3093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="160" mass="17467" checksum="44E9520CD58A5F2F" modified="2014-01-22" version="1">MTAEPSARTTAPRRAYAERLHIPWFAWPAGLGVTALLAAEVWMGSSGLRAWVPFAVLLPVALLLLWRADRIRVVVTDTELHVDDARLPLSVIADVIALDADGKREVLGVGSHPLAFVVQRPWIKGGVQVVLDDPADPTPFWVVSSRRPVELATALLRAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBP8</accession>
    <name>U5WBP8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-N-acetylhexosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_41770</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41770"/>
    <dbReference type="EnsemblBacteria" id="AGZ46608">
        <property type="protein sequence ID" value="AGZ46608"/>
        <property type="gene ID" value="AFR_41770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8453"/>
    <dbReference type="eggNOG" id="COG3525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007082_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102148">
        <property type="term" value="F:N-acetyl-beta-D-galactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015882">
        <property type="entry name" value="HEX_bac_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="PTHR22600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02838">
        <property type="entry name" value="Glyco_hydro_20b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="SSF55545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665766" description="Beta-N-acetylhexosaminidase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_20b" evidence="7">
        <location>
            <begin position="34"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_20" evidence="6">
        <location>
            <begin position="171"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="524" mass="56503" checksum="047E4363A1E54D90" modified="2014-01-22" version="1">MSRMFLAAALGLPVVVAPTAASAAPPSGSPALTSVIPAPAEVSPASGSFRLGPLTVIRTQSGSAAAKAVGEQLARALRPSTGYPLPVVAITPRVLPTISLLLGANESRLGDEGYRLQVDARAVTIRANKPAGLFAGTQTLLQLLPKEIDSPTFVRRSWNVAGGSIVDFPRFAYRGAFMDEARHFHTPAEIKAYIDEISRFKINHLHLHLADDQGWRIQIDSWPKLTTVGGGPGTGVDGVGPGFLTKADYRDLVRYAAARYVTIVPEIDMPGHVNAAQVAYPELTCDGVAPPPRTDTEVGYSSLCISSETTYRFVEDVIRELAAITPGPYLHIGGDEAHATTDADYLTFQQRVLPLVTKYGKTAYGWNEIAKSPASSNAVAQYWDTATTNPTVAAAVTRGTKVIMSPANKAYLDMKYDRNTPLGLSWAGYIEVQTAYGWDPGTHVTGIPESAILGVEAPLWSETLRSIDDIEFMAFPRLPAIAELGWSPATTHDWPSFASRLGAYGPRWTLRDVNFYPSPQITWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBS1</accession>
    <name>U5WBS1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562891.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41525"/>
    <dbReference type="EnsemblBacteria" id="AGZ46559">
        <property type="protein sequence ID" value="AGZ46559"/>
        <property type="gene ID" value="AFR_41525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8402"/>
    <dbReference type="eggNOG" id="ENOG5031XS6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2490842_0_0_11"/>
    <dbReference type="OrthoDB" id="2008997at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="86" mass="9249" checksum="586BD90C1A5A57E9" modified="2014-01-22" version="1">MASVEEVKANVAASVDGTQRAVASILQVSDQLDEALTRLRMTAIGSFHPSIAMAIAQLEQARNRLDEAQTLARSAMDSADAYRMIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBV3</accession>
    <name>U5WBV3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-hydroxy-3-oxopropionate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42095"/>
    <dbReference type="EnsemblBacteria" id="AGZ46673">
        <property type="protein sequence ID" value="AGZ46673"/>
        <property type="gene ID" value="AFR_42095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8518"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_0_7_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="NADP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_binding_11" evidence="2">
        <location>
            <begin position="140"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="24313" checksum="62C87177F1D5A9B8" modified="2014-01-22" version="1">MGAAITRRLLSRGHEVTVWNRTEERMKPLVAAGAKAASAPADVDADVVITMLTDAAAVRDVVAATSLRPGTVLVEMSTIGPEAVRELRVPEGVDLVDAPVLGSVGAAEAGQLNILAGGAVDHARPVLEALGEVHDCGPLGSGAALKLVANTALGTGLAALAETVAVARAANVDIDTALSVLAHGALGGAVRRAQQPDAAFPISLAVKDLDLALGLVSSPFVEAAAAVLRAAPDQSADLGTVVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC47</accession>
    <name>U5WC47_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563050.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42325"/>
    <dbReference type="EnsemblBacteria" id="AGZ46719">
        <property type="protein sequence ID" value="AGZ46719"/>
        <property type="gene ID" value="AFR_42325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8566"/>
    <dbReference type="HOGENOM" id="CLU_2646305_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="76" mass="7868" checksum="7807A6BA706DDF97" modified="2014-01-22" version="1">MKEGLIVTVPLDPEEGATHAFAQVVGFLPVGGVLVVHPETSAGVYAPEDLTVQDPRSVPPAILAAIVKRTSIQPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDB7</accession>
    <name>U5WDB7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein pmbA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_38805</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562349.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38805"/>
    <dbReference type="EnsemblBacteria" id="AGZ46015">
        <property type="protein sequence ID" value="AGZ46015"/>
        <property type="gene ID" value="AFR_38805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7852"/>
    <dbReference type="eggNOG" id="COG0312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046126_0_0_11"/>
    <dbReference type="OrthoDB" id="958356at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD_C" evidence="3">
        <location>
            <begin position="218"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="458" mass="47278" checksum="A9444F31570774F7" modified="2014-01-22" version="1">MSAELDIAGRVIELTRRLAGTGAEAEAVADHSAQALTRFANSAIHQNVADATTTVWLRLHLDGRTAAGSTTVTDADGLQSLVERTIAASRLSPPDAAWPGLTAPAPLRPVEAFDEATAAASPRERAERVRDFVQAADGLETAGFCRTAYSSGGFVNTAGQSVEGRAAEAAMDGIARLDGADGVSRRASEHLADLDGAALGARAAAKARAGGRPIELPPGRYEVVLEPTAVADLLSNLATFGFNGKSFAQHQSFAELGTQQFDPAITITDEPLGATLPFDLEGTPRDTLVLVRDGITTAVSHDRTSAAEAGATSTGHASGSSRSWGPIAANVRLAPGPATGAAADTGDGPTAASARALVSGIRRGLLITDFWYTRVLDQKSLVITGLTRNGVWLVEDGEVTAAVGNMRFTQSYPDALGPGSVLGIGAEAIPLPDRWSGVRYTAPALHLKSWNLTGNASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDH0</accession>
    <name>U5WDH0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38710"/>
    <dbReference type="EnsemblBacteria" id="AGZ45996">
        <property type="protein sequence ID" value="AGZ45996"/>
        <property type="gene ID" value="AFR_38710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7833"/>
    <dbReference type="eggNOG" id="ENOG5030HTK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_665046_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025519">
        <property type="entry name" value="DUF4407"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14362">
        <property type="entry name" value="DUF4407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="422" mass="46196" checksum="42CDAC91A73B4332" modified="2014-01-22" version="1">MLLRIANVNPDSVTTHSDRVLYSSIGVFIVLYFAYATVGGAAFVDASANYTHPWYQWLVGPLVATGVVAYDRAVVGRVAINYERLDSPDPEDLLRKPTFGLYAGRLALALLFAVIITEPLMLSRYQGEIDARLNEVHNEQISKVEVSGAIAAYDARLRDLKKQTVAENRAVQALNTRAAEKRRDARKLYDQAIADSEGNGVTRRSGCPDGGYCDTLVQRSRGLDDQAARLDAQAARLQDTQKAARTARAAEEADLTTKIADQRTVNAESIRADAGFGARTKAMWYLVNSDFWGIGVFYLGVALLLVALDCAAVGLKFVSHGNAYERTEAREARVHEHEAAIGYEQGLKDVRRYAEATSRVMAEGIGKASRDEQIADVAIERARAHLYDSVAGSALPPLPPIPLIERHPAAPRTQRNVDSLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE28</accession>
    <name>U5WE28_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562509.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39610"/>
    <dbReference type="EnsemblBacteria" id="AGZ46176">
        <property type="protein sequence ID" value="AGZ46176"/>
        <property type="gene ID" value="AFR_39610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8013"/>
    <dbReference type="eggNOG" id="ENOG502ZHN2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1912603_0_0_11"/>
    <dbReference type="OrthoDB" id="1973317at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="131" mass="13978" checksum="85C9028553B03D6C" modified="2014-01-22" version="1">MGTLVTLHLPPDSPVADLPWIITIGALDDNEEWEPLVCGPYERPHALALAQAVVADEDLMAVVEPLLPLAGADAIRDEIELARAGHNPDLDAEDFDADDEPRRTTPGRPPAEAEVRAGFARIAAKLTSAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFH2</accession>
    <name>U5WFH2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42060"/>
    <dbReference type="EnsemblBacteria" id="AGZ46666">
        <property type="protein sequence ID" value="AGZ46666"/>
        <property type="gene ID" value="AFR_42060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8511"/>
    <dbReference type="HOGENOM" id="CLU_165910_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="13863" checksum="23994A7B1863908A" modified="2014-01-22" version="1">MIPTRVTEHVHCARRRRAGATRTWSLAAPIIRSGRIQLAVPRWPAHVFAETDYCYGIGPLTLRVDRVEWTKPIPYEGDTWLEVEGTVIDPTGREGSRRQVLVRARRLPHPPTRKRPRLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VN44</accession>
    <name>U5VN44_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative sodium/proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_00545</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357342.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00545"/>
    <dbReference type="EnsemblBacteria" id="AGZ38398">
        <property type="protein sequence ID" value="AGZ38398"/>
        <property type="gene ID" value="AFR_00545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.111"/>
    <dbReference type="eggNOG" id="COG0475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_4_1_11"/>
    <dbReference type="OrthoDB" id="941006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="14"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="41166" checksum="21B7E729B36D2392" modified="2014-01-22" version="1">MHSTTILLIEVGALLFALGMLGRIGRRFGLSPIPLYLLAGLAFGHGGVIPLSASEEFIEVGAEIGVILLLVMLGLEYSASELVGNLRSAAPAGLIDALFNALPGAGFAFLLGWGWEAALVLAGITWVSSSGVIAKVLGDLGRLGNRETPVILSVLVIEDLAMAFYLPLVTAVLAGVGLLGGAKALAVAVLTVVAVLVVAIRYGHAISSFISVKDAEALLLSVLGLTLFVAGVAAELKVSAAVGAFLVGIALSGPVAHNATQILSPLRDLFAAVFFVFFGLSTDPAAMPPVILPALGLAVLTMLTKVLTGVLASRRAGIALPGQIRTGLALMPRGEFSIVIAGLAVASGVEPELAPLATAYVLITVVSGPMLARLPDYTWFKSMVRKRMAVQRPVGPVTAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNM5</accession>
    <name>U5VNM5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chaperone protein ClpB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">clpB</name>
        <name evidence="13" type="ORF">AFR_00565</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer; The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357346.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00565"/>
    <dbReference type="EnsemblBacteria" id="AGZ38402">
        <property type="protein sequence ID" value="AGZ38402"/>
        <property type="gene ID" value="AFR_00565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.115"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_2_11"/>
    <dbReference type="OrthoDB" id="44062at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017730">
        <property type="entry name" value="Chaperonin_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03346">
        <property type="entry name" value="chaperone_ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Clp R" evidence="12">
        <location>
            <begin position="3"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="414"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ38402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="862" mass="94061" checksum="73D17F0A79161176" modified="2014-01-22" version="1">MNAERLTTKSRDVITGAVADANQRGHATVEPWHLLLSLLDTGGSTAGALLRAVGANPADVRRAAVRAVEQQPSARGASTAEPSLSREFVNAIGEAELIAKPLGDEYVSTEHLLAGLARVGGVVGKALRDVGATEETLVAAFPSVRGGDRRVTTAEPEQTYKALEKYSVDLTALARDGKIDPVIGRDAEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVAGDVPETLRDKKLVSLDLGAMVAGAQYRGQFEERLKSVLEEIRNSDGQVVTFLDELHTVVGAGKGEGSMDAGNMLKPMLARGELRMVGATTLDEYREHIEKDPALERRFQPVVVGEPTVEDTIGILRGLKGRYEAHHRVQITDAALVAAAALSDRYISDRFLPDKAIDLIDEAASRLRMEIDSQPVELDQLQRQVDRMRVEKLALEKETDPASIARLERLERDLADREEELTALSARWERERSGLNRVGELKKRLDEQQADLERAQRDAEWEKASRLQYQEIPALMAEIEHASAAEEEKTEPPMVKEEVGADDIAEVIASWTGIPAGRMMEGETAKLLRMEETLQAKVVGQPIAVAAVSGAVRRARAGVADPDRPTGSFLFLGPTGVGKTELAKALAGFLFDDERAMVRIDMSEYGEKHSVARLLGAPPGYVGYEEGGQLTEAVRRRPYSVVLLDEVEKAHPDVFDTLLQVLDDGRLTDGQGRTVDFRNAILVLTSNLGSSVISDFTLGEEERREEVMASVRAHFKPEFLNRLDDIVVFQALTSQDLAAIVDIQLGRLRSRLADRRLELEVSEAAVLWLGERGYDPIYGARPLRRLVQSSIGDSLAKALLAGEIVDGNTVVVDLNDTKDGLSVHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNN4</accession>
    <name>U5VNN4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_00615</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00615"/>
    <dbReference type="EnsemblBacteria" id="AGZ38412">
        <property type="protein sequence ID" value="AGZ38412"/>
        <property type="gene ID" value="AFR_00615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.124"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="11"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="102"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="162" mass="18541" checksum="F98CF076A53E8FE0" modified="2014-01-22" version="1">MPVTYEEFVDSRLGALLRYAVMLTGDEHTAQDLVQDTMVRVQLNWRRVVRSDSPDGYVRKMLTNQFIDLRRGSWIKRVLLRAEPDPVRAEPSDHASETAERDSVWTMLARLPRQQRAVLVLRYYEDLPDAEIAEVLGCAVGTVRSSISRALATLRTELVEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPW4</accession>
    <name>U5VPW4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357941.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03545"/>
    <dbReference type="EnsemblBacteria" id="AGZ38998">
        <property type="protein sequence ID" value="AGZ38998"/>
        <property type="gene ID" value="AFR_03545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.713"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1694240_0_0_11"/>
    <dbReference type="OrthoDB" id="1914312at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="17108" checksum="F4F3E236EA137E2F" modified="2014-01-22" version="1">MTIDMRMTRQLPATPEEVFDAYTDAEKQKIWFSILDEQPGIVEIEVDLRVGGQQTAVWGPGPDTLFRETQTFLEIDRPHRLVTESTGSSPDGMSMTTRIEVTFEEKDGGTLMTVAQSGFPVPEVRDFFVGEVWVGAFDRLEAYVTRGKASADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ53</accession>
    <name>U5VQ53_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_03290</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357890.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03290"/>
    <dbReference type="EnsemblBacteria" id="AGZ38947">
        <property type="protein sequence ID" value="AGZ38947"/>
        <property type="gene ID" value="AFR_03290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.662"/>
    <dbReference type="eggNOG" id="COG1139">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027059_2_0_11"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019516">
        <property type="term" value="P:lactate oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004452">
        <property type="entry name" value="4Fe-4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024569">
        <property type="entry name" value="4Fe-4S-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153">
        <property type="entry name" value="PTHR47153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11870">
        <property type="entry name" value="DUF3390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00273">
        <property type="entry name" value="TIGR00273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="LUD_dom" evidence="8">
        <location>
            <begin position="86"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="324"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3390" evidence="9">
        <location>
            <begin position="403"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ38947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="472" mass="50944" checksum="94833E5406ECA9F5" modified="2014-01-22" version="1">MSTFLGMPATAPRGVGNLRGDESFPAAARKALGNDQLRRNLGHATTTIRAKSGAVIGELPDWQELRSAGSALKAATMARLPELLLQLEENVTRAGGVVHWAADANEANRIVTELVRATGEQRVIKVKSMATQEIGLNEALEEAGIEPIETDLAELIVQLGNDKPSHILVPAIHRNRSEIREIFLREMPGVDPALTDEPVVLAGAARRYLRETFLSTKVAVSGANFAIADTGTLVVLESEGNGRMCLTLPETLITVMGIEKLVPTWQDLEVFLQLLPRASTGERMNPYTSMWTGVTPGDGPQSFHLVLLDNGRSAVLADEVGRQALHCIRCSACLNVCPVYERTGGHAYGSVYPGPIGAVLSPQLTGVEDNASLPYASSLCGACFDACPVKIDIPSILVHLRNEVPHPRTERAAMAAAAYTMDHPKLYAAAQHAAKLTKLAGHRLPPPLNGWTASRDLPEPPPETFRDWWAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQN1</accession>
    <name>U5VQN1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_04040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358042.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04040"/>
    <dbReference type="EnsemblBacteria" id="AGZ39097">
        <property type="protein sequence ID" value="AGZ39097"/>
        <property type="gene ID" value="AFR_04040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.816"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_13_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="11"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="406" mass="42165" checksum="09B7584B549C0AA7" modified="2014-01-22" version="1">MRALFPRLGADFSKLWTASAVSNLGDGITMAAGPLLVASITTDPAAVAGAVFAQQLPWLLFALLSGAWADRLDRRRLVVTVNLVRAAALAGLVAATATDQVTVPVIYAVFFLLGTGETLSDTASSAFVPALVPSDRLPTANAWLGATFTVINQFAAKPLGAWLFVIAAAVPFGVNALTFAASAALIASIRSLPAPPARTRTSLRADIAEGVRWLWRHRLLRTLAVTMAFSNVIYCAAFAIFVLYAFERLGLGQVGYGVLLTAFAVGGLAGTVVAPRLLTTVDTTLLLRAGLFVEVALHGTLALTRNPFVAAAMIVVFGVHTMIWGNIVITLRQRAVPAALYGRVTSVYSLLDLGGAALGSLLGGAVAQAYGIVATFGTASAAMAVVLLLAWRSLGAGVPGGHIVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQZ2</accession>
    <name>U5VQZ2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Transcription elongation factor GreA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Transcript cleavage factor GreA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">greA</name>
        <name evidence="10" type="ORF">AFR_05520</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GreA/GreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358924.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05520"/>
    <dbReference type="EnsemblBacteria" id="AGZ39393">
        <property type="protein sequence ID" value="AGZ39393"/>
        <property type="gene ID" value="AFR_05520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1120"/>
    <dbReference type="eggNOG" id="COG0782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101379_0_0_11"/>
    <dbReference type="OrthoDB" id="1536415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00829">
        <property type="entry name" value="GREAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GreA_GreB_N" evidence="9">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="GreA_GreB" evidence="8">
        <location>
            <begin position="83"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="164" mass="17567" checksum="ACF0D417BE1B5079" modified="2014-01-22" version="1">MTSSEASKTWLSQDAYDRLQAELDELIAARPAVAAEINARREEGDLRENGGYHAAREEQGKQEGRILYLKEFLRKAEVGDAPSADSVAPGTVVTIYFDEDKSDTETFLLGSREIAATTDYTVYSPESALGKAILGAGPGQTVTYTAPSGADIKVTVVEFAPFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR27</accession>
    <name>U5VR27_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358959.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05695"/>
    <dbReference type="EnsemblBacteria" id="AGZ39428">
        <property type="protein sequence ID" value="AGZ39428"/>
        <property type="gene ID" value="AFR_05695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1155"/>
    <dbReference type="eggNOG" id="COG3284">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037518_1_0_11"/>
    <dbReference type="OrthoDB" id="685227at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="118"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="434" mass="46592" checksum="89F8B2F98ACD9197" modified="2014-01-22" version="1">MSNPWLALTLGADPTERISQVGRAHERFVTGEQAVTQVRSVVADSWRRSAGALVNPDSTAPIELSDGDLEAYRAAHPLARVLPIFRDLLGGLADDGDHMMAVCDAHGRLLWVEGTASVLRGAESMNFVPGARWDEAHAGTNAPGTALAVDHPLQIFATEHFSRGVQRWTCAAAPIHDPATGRLLGAIDVTGGDHLANPHSLALVRATALAAEAYLSAHQPAPEKHAATVSVSGREDAVLSVGGRRLRLGRRHSELLVLLLVHPEGRTGDQLAFDLYADDLNPVTVRAELSRLRRILGPELLDSRPYRLRAELGADFLTVTRLLECGLVAEALAAYDGPLLPSSDAPGIVRLRRLIDSRLRAAVLTGGDPRLIQTWVGAPWGADDLEMWEAYATALPPGSPARPHALNRVVELAGEYGLRPSVSGTPKMTRRLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRI7</accession>
    <name>U5VRI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative FtsW/RodA/SpoVE-family cell cycle protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_00280</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00280"/>
    <dbReference type="EnsemblBacteria" id="AGZ38345">
        <property type="protein sequence ID" value="AGZ38345"/>
        <property type="gene ID" value="AFR_00280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.57"/>
    <dbReference type="eggNOG" id="COG0772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029243_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="483" mass="51181" checksum="B3FF327F911E9DD3" modified="2014-01-22" version="1">MPRVPGTKTGRNAELGLLAFAMVIVAAYGATVEANQYDKITPTFWVPAALLSVLFLGLHLVVRFVAPHADPVLIPAVALINGLGVGFLRRLDIAKAISEKQPELGVFAGVGGRQLAWTLVALVLAAVLLLIVRDHKTVSRYAYTLGLVGIVLVMIPAVLPSSISEVNGAKLWIKMGAFSIQPGEFAKLALVSFFAYYLVRKREVLSLASRRFLGIDFPRGRDLGPVVTVWLLSLLVLVFEKDLGTALLYFGLFVVTLYVATERASWLIIGLVMFFGGVYVAYLLGASIGGPFANFYDRATVWLDPFASENYERIGGSYQLVQGLLGLGTGGMFGTGPGAGSPGFVPEVRNDFIFAGMGEEIGLFGLSALLVCYLLIVERGLRAALGVRDSFGKLVAGGLAFTLGLQVFVILGGITGLIPLTGQTTPFLSSGGSSLMANWLLIAMLLRISDAARKPANGGPAASGGPKQPPAQLHGAMTEVIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRJ2</accession>
    <name>U5VRJ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357294.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00305"/>
    <dbReference type="EnsemblBacteria" id="AGZ38350">
        <property type="protein sequence ID" value="AGZ38350"/>
        <property type="gene ID" value="AFR_00305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.62"/>
    <dbReference type="eggNOG" id="ENOG5033BI6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186178_0_0_11"/>
    <dbReference type="OrthoDB" id="2090823at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="10079" checksum="0CDA180E4176051C" modified="2014-01-22" version="1">MYAWIWRKLPFGLWGKLTGSLAMAAAIGALLWYVVFPWATPLLPFDDVQVGTGTEQGGPAEQDPNAVPGADDPGLGPDEIPYNEHTNDPEPTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRV0</accession>
    <name>U5VRV0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease adapter protein ClpS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">clpS</name>
        <name evidence="3" type="ORF">AFR_06325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds to the N-terminal domain of the chaperone ClpA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359085.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06325"/>
    <dbReference type="EnsemblBacteria" id="AGZ39554">
        <property type="protein sequence ID" value="AGZ39554"/>
        <property type="gene ID" value="AFR_06325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1283"/>
    <dbReference type="eggNOG" id="COG2127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153743_1_0_11"/>
    <dbReference type="OrthoDB" id="1829294at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00302">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022935">
        <property type="entry name" value="ClpS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33473">
        <property type="entry name" value="PTHR33473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ClpS" evidence="2">
        <location>
            <begin position="20"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="96" mass="10875" checksum="68669FCB452AF71A" modified="2014-01-22" version="1">MALPQVAPAETPQIEEVPADDRPWVTIVWDDPVNLMSYVTWVFQKLFGYSHDKAEQLMMDVHNKGKAIVSTGARERMEMDASQLHGYGLWATVDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS33</accession>
    <name>U5VS33_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06825"/>
    <dbReference type="EnsemblBacteria" id="AGZ39654">
        <property type="protein sequence ID" value="AGZ39654"/>
        <property type="gene ID" value="AFR_06825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1394"/>
    <dbReference type="HOGENOM" id="CLU_2756778_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021284">
        <property type="entry name" value="DUF2750"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11042">
        <property type="entry name" value="DUF2750"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="75" mass="8151" checksum="BF55698D380E24B1" modified="2014-01-22" version="1">MPFWSKQSRAKRVVGAVPAYQGFAVVEIPVSDFLDSWLPGLQRDGLLVGVNWAGARATGYDMAPTQVAGWFAELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS49</accession>
    <name>U5VS49_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-N-acetylhexosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_06925</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359205.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06925"/>
    <dbReference type="EnsemblBacteria" id="AGZ39674">
        <property type="protein sequence ID" value="AGZ39674"/>
        <property type="gene ID" value="AFR_06925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1414"/>
    <dbReference type="eggNOG" id="COG3525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007082_5_1_11"/>
    <dbReference type="OrthoDB" id="727559at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102148">
        <property type="term" value="F:N-acetyl-beta-D-galactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015882">
        <property type="entry name" value="HEX_bac_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="PTHR22600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02838">
        <property type="entry name" value="Glyco_hydro_20b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="SSF55545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_20b" evidence="6">
        <location>
            <begin position="13"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_20" evidence="5">
        <location>
            <begin position="146"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="498" mass="54103" checksum="4CD4F265CA497F0C" modified="2014-01-22" version="1">MSEPVFERRGLGDVIPAPAEVRPDQAVTFTIDEGTAIRTAPGSPEAHRVGEHLAAVLRPATGFALPVGAGDGTIALLLDGDDTLGDEGYELEIAADGVTARAAAPAGLFAAVQTIRQLLPAEIEAGTPQAGAWTLPGGRITDRPRFAYRGAMLDIARHFFGVDEIKSYVDSLVQFKINHLHLHLTDDQGWRLEIDGWPLLTEISGGAGTGVDGTGPGFLTKADYTELVAYAEERFVTIVPEIDMPGHVNAAQHAYPELTADGVAVPQRTDSEVGYSSFVAGKEETYAFLDDVIREVAALTPGPYLHIGGDEAQATTAEDYRTFIQRVLPLVAKYEKRVVGWHEMAGVELPETAIPQYWRIEATDEGTARAAANGSKVIMSPADRTYLDMKYTAESPLGLDWAGTIDVERAYGWDPADRLPGVGEEHLLGVEAPLWSETLRSLTDVETMTYPRLPAIAEIGWSPRVTHDWESFRTRLAAFGPRWRRQGVAFHASPEIPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSQ3</accession>
    <name>U5VSQ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01860"/>
    <dbReference type="EnsemblBacteria" id="AGZ38661">
        <property type="protein sequence ID" value="AGZ38661"/>
        <property type="gene ID" value="AFR_01860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.376"/>
    <dbReference type="eggNOG" id="ENOG5033B4A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157938_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025338">
        <property type="entry name" value="DUF4244"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14029">
        <property type="entry name" value="DUF4244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="63" mass="6623" checksum="DB80EA85AD4EADBA" modified="2014-01-22" version="1">MHRFTIRALTARFAGLHDDEGMNTAEYAVGTLAAVAFAGVLYKVVSSPSVQAALTGVINEALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSZ8</accession>
    <name>U5VSZ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359609.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40078">
        <property type="protein sequence ID" value="AGZ40078"/>
        <property type="gene ID" value="AFR_08945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1820"/>
    <dbReference type="HOGENOM" id="CLU_2535034_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="83" mass="9025" checksum="661AA005D9662F0C" modified="2014-01-22" version="1">MSATELQLLEAKLLAATWASEIAAIAQQAPTAKAAVEELMTRYSLHVDQAVTIMDSPFRFVTADVRERLEAEIAVSKAAHPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT03</accession>
    <name>U5VT03_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Threonine dehydratase</fullName>
            <ecNumber evidence="4">4.3.1.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_08970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359614.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08970"/>
    <dbReference type="EnsemblBacteria" id="AGZ40083">
        <property type="protein sequence ID" value="AGZ40083"/>
        <property type="gene ID" value="AFR_08970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1825"/>
    <dbReference type="eggNOG" id="COG1171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_2_11"/>
    <dbReference type="OrthoDB" id="1876944at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:L-threonine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="17"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="30721" checksum="AAA9AEEF738B2256" modified="2014-01-22" version="1">MTPTSEVEAAAQRIAGRVRRTPVLQDGPVLLKLEFLQHAGSFKTRGMFNKILTAAERGLPEAGVIAASGGNAGLAAAYAARELGVPAEIYVPVTAPPVKVAKLSKLGARVTQTGSEYAEAYAAAIVQAEVTGALFCHAYDDPDMVAGNGTLGLELLEQLPDGFDTVLVAVGGGGLIAGVAAALDGRAKVVAVEPRTSSALHAALAAGGPVDVPVSGVAVDSLGARRAGTIAYTTAVAAGLPSVLVEDQAIVDARRQLWEDYRVAVEHGTAAAQAALTSGAYVPAPGERVVVLLCGANTNPADLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTS2</accession>
    <name>U5VTS2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative cation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_04030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04030"/>
    <dbReference type="EnsemblBacteria" id="AGZ39095">
        <property type="protein sequence ID" value="AGZ39095"/>
        <property type="gene ID" value="AFR_04030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.814"/>
    <dbReference type="eggNOG" id="COG0569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="28"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="3">
        <location>
            <begin position="158"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="240" mass="25602" checksum="C7573D6C7C1F9D40" modified="2014-01-22" version="1">MADKNADKNADRNAAKNAEKKPGTGPDNVVVIGLGRFGGAVADSLVNLGHEVLGIDSNAHIVQDWSDRLTHVVEADATDTEAMRQLGVQDFSRAVVGIGTHLEASVLTVLTLAEIGVPEIWAKAISVKHGKILSSVGARHVIFPESEMGERVAHLITGRMLDYIEFDDGFAIAKVRSPQEAIGRTLADTSLRTKWGVTVVGIKLPGEDFTYARPETVVPPGCILIVAGDTSKVERFAAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTY2</accession>
    <name>U5VTY2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpmG</name>
        <name evidence="5" type="ORF">AFR_04405</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_015618978.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04405"/>
    <dbReference type="EnsemblBacteria" id="AGZ39170">
        <property type="protein sequence ID" value="AGZ39170"/>
        <property type="gene ID" value="AFR_04405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.891"/>
    <dbReference type="eggNOG" id="COG0267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190949_0_2_11"/>
    <dbReference type="OrthoDB" id="2034291at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00294">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="L33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001705">
        <property type="entry name" value="Ribosomal_L33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018264">
        <property type="entry name" value="Ribosomal_L33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43168">
        <property type="entry name" value="PTHR43168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00471">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01023">
        <property type="entry name" value="rpmG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00582">
        <property type="entry name" value="RIBOSOMAL_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="55" mass="6640" checksum="6EFAF7F24679FC5C" modified="2014-01-22" version="1">MAKATDVRPKITLACTECKERNYITRKNRRNDPDRIELKKFCPRDGRHTLHRETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU48</accession>
    <name>U5VU48_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cupin 2 barrel domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_04730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04730"/>
    <dbReference type="EnsemblBacteria" id="AGZ39235">
        <property type="protein sequence ID" value="AGZ39235"/>
        <property type="gene ID" value="AFR_04730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.955"/>
    <dbReference type="eggNOG" id="COG5553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130991_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="104" mass="11423" checksum="FF2619C058147911" modified="2014-01-22" version="1">MTEQVLSPVGQEIMLENDKVRIWHITLAPGETQPLHHHGLAYVVVAVQGAKNVIHTAAGERIDVEEKTGAVVFREPGQTHMLTNAGDTVYIGRIIELKTGFDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUB5</accession>
    <name>U5VUB5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">6-phosphogluconolactonase</fullName>
            <shortName evidence="7">6PGL</shortName>
            <ecNumber evidence="5 7">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pgl</name>
        <name evidence="9" type="ORF">AFR_10615</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="5 7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360263.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10615"/>
    <dbReference type="EnsemblBacteria" id="AGZ40412">
        <property type="protein sequence ID" value="AGZ40412"/>
        <property type="gene ID" value="AFR_10615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2164"/>
    <dbReference type="eggNOG" id="COG0363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053947_1_0_11"/>
    <dbReference type="OrthoDB" id="1828393at2"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00409"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="PGLS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="PTHR11054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine_iso" evidence="8">
        <location>
            <begin position="10"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ40412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="25729" checksum="588C1C606BCDC92F" modified="2014-01-22" version="1">MSETVVVVVPDADVLASAVSARLVIKIIDAQATRGWASIVLTGGRVAAKVLRAVKELPASAAVDWSRVDLWWGDERFLPSGDPDRNETQAREALLDSLPLDPAQVHAMPPSDGPDGDDAKAAAARYARELAAAARPDGSELPPFDVLMLGVGEDGHVASLFPGHPGLSETGTTAAVHDSPKPPPTRVTLTMPTIRTADEVWLIAAGPDKAAAVRTAIEGGADPVPAASATGTSKTLFLLDTAAAADLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUD0</accession>
    <name>U5VUD0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360539.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11345"/>
    <dbReference type="EnsemblBacteria" id="AGZ40558">
        <property type="protein sequence ID" value="AGZ40558"/>
        <property type="gene ID" value="AFR_11345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2312"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148715_1_1_11"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="114" mass="12447" checksum="611940B2877E9A11" modified="2014-01-22" version="1">MTNDSLPTTVRGFLTAHIAGDADTASSFFAEDAVAVDQGESFRGREQIHAFLRDGGSEFEYTTEQIGAAHIDDDHWVVTLRLEGTFPGGVADLDYRFTIREDVVTELVIANHGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV43</accession>
    <name>U5VV43_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39560">
        <property type="protein sequence ID" value="AGZ39560"/>
        <property type="gene ID" value="AFR_06355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1289"/>
    <dbReference type="eggNOG" id="ENOG5031TMN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1902202_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="12799" checksum="172C5A6423782B2B" modified="2014-01-22" version="1">MVPFGWETGPADAPEPIDGEEVYFRFPGVDDPAPGTRRLLAMSIYASFLGLAGVGVGIRGLVSQIGGGVPGWYVPVLAFLGMVSVAFSVGAFLSIHRRVLPWLLLLGAAVPLIADVMLAVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV49</accession>
    <name>U5VV49_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360937.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40878">
        <property type="protein sequence ID" value="AGZ40878"/>
        <property type="gene ID" value="AFR_12960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2632"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_0_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="40"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="31723" checksum="CC7C8DFEC0B3B3FD" modified="2014-01-22" version="1">MATRSTLNTALSRFLRSRRDALTPAQAGMVPFPGPRRVPGLRKEELAVLAGISPDHYSRLEQGRQTTLSDEMCNALARALRLDDVERRHLRALARPASPRGEWETPQRAEPGLLRILAALDHLPGLLLGRRSEILARNLLLDAVLGVSLPIGAAFVRWLLLDPAARERIVNWDEFGAAAVGGLRFETGRHPHDRRLSALIKQLRSHSDEVARWWDDQTVTDQTSLTKRLAHPAAGRLEFAIETVTLPHCPDQRLVVYTVAPDSPTADVLPLLSGWGADALIGGPHPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV60</accession>
    <name>U5VV60_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360746.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12000"/>
    <dbReference type="EnsemblBacteria" id="AGZ40687">
        <property type="protein sequence ID" value="AGZ40687"/>
        <property type="gene ID" value="AFR_12000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2443"/>
    <dbReference type="eggNOG" id="COG3371">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080422_1_1_11"/>
    <dbReference type="OrthoDB" id="1497902at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665789" evidence="2">
        <location>
            <begin position="22"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="228" mass="23787" checksum="112C7CC95BA47624" modified="2014-01-22" version="1">MTKIRPLAAATFLIGPVIYLAAEAIAAAAWKTPSYSYADNWISDLGSATAGVFQGRELDSPLHVVMNSGFIVQGLLFGLAAVLLSRTLSGRPRTFTAVTGIVTMIGYILVGTFHGSLQAQQNGTLALHFTGATLAIVGGNVLALVLGLHWRKAPETRLIGLVSIVLGAFGLVAVVALFLTFDAGLPSGAIERGSVYTIVIWQFAVAIALLRGRAESRAPRVAATAVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV97</accession>
    <name>U5VV97_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360791.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40732">
        <property type="protein sequence ID" value="AGZ40732"/>
        <property type="gene ID" value="AFR_12230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2486"/>
    <dbReference type="eggNOG" id="ENOG50337K6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121444_0_0_11"/>
    <dbReference type="OrthoDB" id="1885779at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="174" mass="19595" checksum="B3473BFD4E7E2187" modified="2014-01-22" version="1">MARFGIQDYEPDWLNGRDSITAAHGRRLAALTGRTLQHVWLVWDLDVDRWFEDAPVLLDFGDARVGIDHQKFDDLCITWNTVDPSRMIEEPSDEDPFNLAWRPEPTPQLAELAGRTLNRVALLEWSGADDDMANGSVAIGLDLGSTWLTIYNALDENGLALGPPDSSYRQHYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV98</accession>
    <name>U5VV98_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_13235</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360992.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13235"/>
    <dbReference type="EnsemblBacteria" id="AGZ40933">
        <property type="protein sequence ID" value="AGZ40933"/>
        <property type="gene ID" value="AFR_13235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2687"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_11"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="214"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="278"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="529" mass="53706" checksum="920C3C74F426DA2E" modified="2014-01-22" version="1">MKLGGAFDNQSLRTKIGAVVLTATISGLVVGAVAITTVQDLNDKGAAAQRQTLAVQAAAGAFGKNIEAFSGNISSLQLYPSLKDRINQSLAGNKQAITDALSVLSTNLAQESGGAQAVAKAQQDWNAYLAFNGGSSTSTTPLTAAQLAEAVKQYDALYNALGTDENTLQASAKAMSEANITESAERAREATVTIAIVLAAGVLLSILLGFRVANRLRGALTGVSRLADGLAEGDLTRVSGVGGRDEVGRMAASLDGAISRLRADVVQLAGNSSTLSDAAEQLTTVSGTVDAAAADAAAQAGSVASAADVVSNNLQVVSTGSEEMGSSIRDISVSTTEASAVAAQAVEVATATNAIVARLGESSAEIATVIKVITSIAEQTNLLALNATIEAARAGELGKGFAVVAGEVKDLAQETAKATEDISRRVRTIQTDTDGAVVAIGEISAIIERINGIQLTIASAVEEQTATTQEMNRTLSDAAAGASDIAVTITGMSDATRRTTDTVSATRHAAGELAVTAGQLKTLVSRFHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVE0</accession>
    <name>U5VVE0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_13435</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361032.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13435"/>
    <dbReference type="EnsemblBacteria" id="AGZ40973">
        <property type="protein sequence ID" value="AGZ40973"/>
        <property type="gene ID" value="AFR_13435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2728"/>
    <dbReference type="eggNOG" id="COG2229">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077970_1_0_11"/>
    <dbReference type="OrthoDB" id="1438064at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="191" mass="21011" checksum="499D604ADBAE07A8" modified="2014-01-22" version="1">MPFNDDSDGFPSALKLLIAGGFGVGKTTFVGAVSEIEPLHTEELLTTASVGTDHLTGIERKSTTTVAMDFGRITISQDHVLYLFGTPGQERFWFMWDELSSGAMGAVVLADTRRLDDCFPAVDFFESRGIGFLVAVNEFDRACRYETEDLRSALDLKPDVPIMLCDARDRRSATGVLISLMQHLLTAHACR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVF9</accession>
    <name>U5VVF9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359137.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39606">
        <property type="protein sequence ID" value="AGZ39606"/>
        <property type="gene ID" value="AFR_06585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1341"/>
    <dbReference type="eggNOG" id="ENOG5031ZV0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_764251_0_0_11"/>
    <dbReference type="OrthoDB" id="1338921at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="362" mass="38802" checksum="E877B69C1E12676E" modified="2014-01-22" version="1">MTAATMTRTRRSAFPTAIEELLPLARDYAAELGTTPSRNQLMRRFRVGDKKAKTLLAGLTPAPLEPEPLPPIIVAVDDRQDDVQAAFGAPSAPVEQAIPDVPELPLTNANAKPFTQYDKPLLTPLVDPAEAALGPWPTATAPPLALDVTIAEPAPRTRLSRYGMGALLTVVGVLLAGVIVAPIALSSQDIIAWAASPIGLGLTGVWPLVTFFALDAAAAVCVGLVVYCAWRGEGAGVFAWLVWAFAGMSAYANYSHSSGSLARDAVWFFPAMSLAGPFLLEIIVRRVRRWVQEGTGQRARHSVSFGFGRWIPGVGALRETYGAWRLARLDGINDASQAVTAYRRLCPDGSIRVLRALRKRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVG2</accession>
    <name>U5VVG2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361052.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13535"/>
    <dbReference type="EnsemblBacteria" id="AGZ40993">
        <property type="protein sequence ID" value="AGZ40993"/>
        <property type="gene ID" value="AFR_13535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2748"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_0_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="275" mass="29814" checksum="914CE734A37B3718" modified="2014-01-22" version="1">MATTALGDFLRLHRPKVTPADANLVTSGVRRVPGLRREEVALLAGMSADYYVRLEQGRERHPSAQVLEALARTLRLDPDAREHLHRLAGLSPRAHPLAATERADPQLRRLLDMWPENPALVLGRAYDVLAANQLGTALFGATGNLAFKVFLEPAARTFYPDWPDVAAHTAAGLRLAWGVAPNDPVLVRVVDTLLARSPDFGALWARQDARGKTSQTKNLLHPEVGPLTLAMQTFDVRAAPGQQLVVYHAEPGSPDAEALTLLGTLEATRIAVPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVL4</accession>
    <name>U5VVL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FMNH2-utilizing oxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359202.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06910"/>
    <dbReference type="EnsemblBacteria" id="AGZ39671">
        <property type="protein sequence ID" value="AGZ39671"/>
        <property type="gene ID" value="AFR_06910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1411"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_5_3_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="29728" checksum="314A1F87EE95A0CE" modified="2014-01-22" version="1">MTNLGAIFLPSNPPERLRDLARLADEAGLEQLWLWEDCFLNSGIAAATAALAWTERLKVGIGIMPVPFRNAALMAMEIATMRRMFGDRAIIGFGHGVQDWMGQVGAKAASPMTLLREYTTAVHSLLRGETVTTQGRYVKLDAVALDWAPDPAPRVLIGATGPKTLQLSGELAGGTILTGGTSPDGVRAAREHIGAAEDHELVVFMPAATGATAEERIARQQEREGVDWPGAAGDAKTVAAAVERLAEAGAATVILQPTNDDPDPEGFIRFVAEEVRPLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVM7</accession>
    <name>U5VVM7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_12880</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12880"/>
    <dbReference type="EnsemblBacteria" id="AGZ40862">
        <property type="protein sequence ID" value="AGZ40862"/>
        <property type="gene ID" value="AFR_12880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2616"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_852373_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="111"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="33382" checksum="448491F05666152D" modified="2014-01-22" version="1">MVTSALTGEVSAFVVLTERHRAGMRATAIAVLGYTDEAEDAVQDAVLTALQRLSDLREPAAAGAWLRQIVRNNCLMRLRQRQPVPVAEPESLLPPADDPRPDEVIERSHARDWVRHAVGLLSPALREVTLLRYFTEFRSYRQIAQLCGIPEETVGSRLRDGRRALTRTLQESSGDAHPEADLEADGHRRLARQHLAAMQGDEYERVIEDWYQPDLSVVALGGLAGDRSALRLMMDYTFSAGVGIRLRDATASGNVLVWETDFLNPASDPDHCPAGSAWLFRMSRGRVARLAVAYDTGVRSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW07</accession>
    <name>U5VW07_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Carbohydrate ABC transporter membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_14355</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361212.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14355"/>
    <dbReference type="EnsemblBacteria" id="AGZ41153">
        <property type="protein sequence ID" value="AGZ41153"/>
        <property type="gene ID" value="AFR_14355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2913"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_0_11"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="68"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="290" mass="31816" checksum="11F3B05959C10D6A" modified="2014-01-22" version="1">MKPGVRLANAFYVMVLPAFLLFFIFHTIPVIQGIYYSFTDSPGYGPANFVGLRNYIALFTDPRVLHAYWFTFLIAAVATVLVNIVSLAIAVGLNGRIKFKNGLRGIYFIPNVLAILVVGYIFNYLFSNSVPALATKIGIDSLSTSLLTKASTAWIAIVILAVWQSAAFNIIIYIAGLQTVPTEMYEASNLDGASAWRQFRSITFPMIAGFFTINMVLSLKGFLQIFDHIVALTGGGPGTDTESVALLIYKGGFQGGEYGYQSANSVIFFIVIVGFSIVQLRVLQRREVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW92</accession>
    <name>U5VW92_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359536.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40005">
        <property type="protein sequence ID" value="AGZ40005"/>
        <property type="gene ID" value="AFR_08580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1746"/>
    <dbReference type="eggNOG" id="ENOG5031V74">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727442_0_0_11"/>
    <dbReference type="OrthoDB" id="2221993at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="18948" checksum="0DE63779050EF9EC" modified="2014-01-22" version="1">MFSRKNTAERTAAQAWDYLSSAMTTAAESTKDAGKHTADLASAKAAVASSKAAKLADEASRRSSKLATQAGKRGSKFADRASKRSSKLADRAGKKSGKLADKATTKLNAASDEAWARANLAANALAGRKPGLPWGLIVGATLVGVALGWAAASTARAALERQAENEELELAETAIVVTPTYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWH4</accession>
    <name>U5VWH4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41132">
        <property type="protein sequence ID" value="AGZ41132"/>
        <property type="gene ID" value="AFR_14250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2891"/>
    <dbReference type="eggNOG" id="ENOG50336CR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181477_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665821" evidence="1">
        <location>
            <begin position="19"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="109" mass="12237" checksum="589F06D871BFEDDD" modified="2014-01-22" version="1">MMLSLIIRSWRGHTAAMARATAASSQWIDDNGVRQPGGEVHAWEPGTNQTVCGLALSRSRLRRFPHVVFDFRATDQITAADEVRYICPRCVAATTRRGERSWTRTAPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWK5</accession>
    <name>U5VWK5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08955"/>
    <dbReference type="EnsemblBacteria" id="AGZ40080">
        <property type="protein sequence ID" value="AGZ40080"/>
        <property type="gene ID" value="AFR_08955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1822"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2748598_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="4"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="70" mass="7555" checksum="9DE08C83B98AC4D2" modified="2014-01-22" version="1">MVLDVDLPGLDGFQLLSLLRQDGPGLRAVFITALWIEDVLGRIEEFAARYVAKPFTGAGLKAVVREALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWM6</accession>
    <name>U5VWM6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_14960</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14960"/>
    <dbReference type="EnsemblBacteria" id="AGZ41274">
        <property type="protein sequence ID" value="AGZ41274"/>
        <property type="gene ID" value="AFR_14960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3038"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="66"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="32422" checksum="30C28B1B482825DA" modified="2014-01-22" version="1">MSSTKRRDDRRAAWWFLLPVSAGFVIFYGYPAARGAWYSVTDYTMLNTPSFVGADNYAKLVQDDQFWHSLQVTAWYVILNIASQTLLALGIAALMHRLTGSVLLRATLLLPWLVPNVTVGLLWMWLLDTNLGFVNHLITSLGLAPVGFLTDATLAMPSVAAINTWAYTGYTALLLYAGMLQIPQHLYESAAIDGAGEWRMFQRITLPLLRPVLALVLVVSLIGSFQIFDTIAVTTQGDPASATRVIYLYIYEQAFKYFHLGYAAAAAVCLALILGVFTAIQMRLLRASRSDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWN9</accession>
    <name>U5VWN9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative acetolactate synthase large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_15705</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15705"/>
    <dbReference type="EnsemblBacteria" id="AGZ41423">
        <property type="protein sequence ID" value="AGZ41423"/>
        <property type="gene ID" value="AFR_15705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3189"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="6">
        <location>
            <begin position="4"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="4">
        <location>
            <begin position="197"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="5">
        <location>
            <begin position="395"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="559" mass="59115" checksum="2CB8C78AA06FFD2B" modified="2014-01-22" version="1">MTPRTGARVVADMLDGYGVSHLFLVPAILRRTLAELERHHPHIDRVVTHGEKAAAYMADGYARVSGRPGVVAAQVVGALNLAAGLREPFLAGSPVIALTGGRTPATKFRQVYQEIDDLPAFEPVTKFNATIDDAGRFPDMLRHAFRVATTGAPGPVHLQIQGNEGQLDTEETDAEPLVEPAFAQVPPVRPAPDDNDVAEVLRLLAGAERPVIVAGGGVRSSGAGAALVALAEALRLPVATSANGKDTFPGTHELSAGVAGTYSRECANQVVSRADLVLFVGTGTGSMTTHFWAVPPSGTPAIQIDIEPAHLGRNYPLRAGLTADARTALTRLLALAPRQPAVDRTAWLAEVDTLKADWRSRYREVLTSDAVPIRPERICAELTAGLPADAVLVVDTGHAGMWMAQFHDITSPAQSYLRSAGHLGWAFSAGVGAKCAAPGRPVVVFTGDAGLYYHLGEIETAVRRKVNLVTVVNNNHGGNQSKRGFDRAYGGQATDQARELWTYEDVDFARIATEMGALGLRVDRPGDLAPALDRALSAGRPVVVDVHTDIGVAAPPPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX42</accession>
    <name>U5VX42_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="1">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_10030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360033.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10030"/>
    <dbReference type="EnsemblBacteria" id="AGZ40295">
        <property type="protein sequence ID" value="AGZ40295"/>
        <property type="gene ID" value="AFR_10030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2044"/>
    <dbReference type="eggNOG" id="COG0325">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059988_0_0_11"/>
    <dbReference type="OrthoDB" id="1423012at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PTHR10146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00044">
        <property type="entry name" value="TIGR00044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01211">
        <property type="entry name" value="UPF0001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_N" evidence="4">
        <location>
            <begin position="11"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1 2">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="237" mass="25043" checksum="C17E4087E959C8A6" modified="2014-01-22" version="1">MTTRRDELAASLDEVRRRIGRACAAVNRDPGSVTLVAVTKTYPASDVVLLAGLGVTDVGENKDQEAAAKAAAVDEPLRWHFVGQLQRNKAKSVVTYADVVESVDSMRLAGALSTAAARLRERPLDVLVQVSIDGDPARGGAIDGAAEPDRDLWRVAAAVQEAEGLRLGGVMAVAPRGWEPARAFADLAGLAERLRADHPDATVVSAGMSGDLEDAVRYGATHVRIGTSLLGMRNTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXJ8</accession>
    <name>U5VXJ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360221.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10510"/>
    <dbReference type="EnsemblBacteria" id="AGZ40391">
        <property type="protein sequence ID" value="AGZ40391"/>
        <property type="gene ID" value="AFR_10510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2142"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_8_2_11"/>
    <dbReference type="OrthoDB" id="1442962at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="21765" checksum="4EB2E1BED203FFC2" modified="2014-01-22" version="1">MDETLQLDPARTAIVLIEYQNEFTSDGGVLHNAVAPVMDKTGMLANTVALVDAARAAGVTIMHAPITFAPGYGELSRHPYGILKGVVDGNAFVKGTWGAAIVDDLTPAEGDILIEGKRGLDTFASTNLDFILRGRGIETVILAGFLTNCCVESTMRSAYENGYRVITLTDCTAATSPEEHDNAIAYDYPMFSLPMESTKVTAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXN9</accession>
    <name>U5VXN9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_16730</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361721.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16730"/>
    <dbReference type="EnsemblBacteria" id="AGZ41624">
        <property type="protein sequence ID" value="AGZ41624"/>
        <property type="gene ID" value="AFR_16730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3392"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="23688" checksum="C70DEFC4E09EAD27" modified="2014-01-22" version="1">MTAPVVRFDAVAKTYPGPPPVHALHAADLTIERGEYVAVVGPSGSGKSTFLNLVGLLDRPSSGCYELDGINVGSLRESDRSAVRGRRVGFVFQSFHLLPYRSAVENVALAQLYTGPPGPERRQTALAVLDRVGLAHRAEALPTMLSGGERQRVAIARALANQPSLLLCDEPTGNLDSETAATVLALLDEVHAAGFTVIVITHDAEVAARSPRRITIRDGVLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY13</accession>
    <name>U5VY13_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_16945</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16945"/>
    <dbReference type="EnsemblBacteria" id="AGZ41667">
        <property type="protein sequence ID" value="AGZ41667"/>
        <property type="gene ID" value="AFR_16945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3439"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="297" mass="31550" checksum="572C6DDFAC0D0769" modified="2014-01-22" version="1">MAPHTRARVREVIDRLGYRPDPVARALASGHGDVIELVVVDDATVFGSSPYYGRVTAGILQELAGTNSQLSVHVVEKPDAAALLTRIADTVSAGVVLVNVAPALAAEFHARCERVVAMSPSAPGVPFVGVENAEGAYAAMVHLHETGRKRIAALHGQEGNPCAEGRRKGHQRAVRDLGIPDVAAAGKFRREAGYELTQRLLAEEPELDAIFVACDLMATGAMQALADAGRRIPEDVAVVGFDDSVIAACSTPPMSSVHQPVEQMAAAATRALVRRQVAPHWQRVFPAELRVRRSSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYN6</accession>
    <name>U5VYN6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Endoglucanase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18920"/>
    <dbReference type="EnsemblBacteria" id="AGZ42058">
        <property type="protein sequence ID" value="AGZ42058"/>
        <property type="gene ID" value="AFR_18920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3843"/>
    <dbReference type="eggNOG" id="COG4447">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004180_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666222" evidence="2">
        <location>
            <begin position="25"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="3">
        <location>
            <begin position="776"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="752"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="882" mass="92315" checksum="B5976E34AC0C4989" modified="2014-01-22" version="1">MRRPFIVLAAAALLVGATTYVAQAAGRPPAAWAAAAAAEPHTWKNVRIDGGGFVPGLIFNRTEKNLIYARTDIGGAYRWNQAGQSWVPLLDWVGQDNWGYNGVLSIATDPVDTDRVYAAVGMYTNSWDPNNGAILRSSDRGATWQVTKLPFKNGGNMPGRGMGERLAVDPHDNSRVYFAAEGGNGLWRSTDFGATWAKVTAFPNAGNYVQDPGDTNGYLSQNQGLTWVTFDDVTRDVYVGVADKENPVYRSTDGGATWARIPGQPTGYLAHKGVLDPVNHYLYTATSDTGGPYEGGKGQVSRFNTVTGEWTDVSPVKLSAGDDLYFGYSGLTVDRQKPGTLIVATQISWWPDAIFFRSTDSGATWTRIWDFTSYPSVSKRYTMDISANPWLDFNTSPQPPESTPKLGWMNESVEIDPFDSDRMLYGTGATVYGTTQLTNWDRNTPFTIKPMARGLEETAVLDLASPPSGAPLVSALGDIGGFHHADLDTVPATFHDTPSLGSNTGLDFAELDPQVFVRVGNADAAPHIGVSTDGGKNWYNGQEPAGVTGGGSVAVSADAGAVVWAPGGAGVHYSTTRGSSWTASTGLPAGAKVESDRVNPKTFYGYAAGKFYASTDGGATFTASTAAMPSTGRVNFHAVPGKAGDVWLAGETGLLHSTNAGTTFTAVAGVTSAINVAFGKAAPGAAYPAVFLVGTVEGVTGVFRSDTGGTSWVRINDDAHQYGNAGDALAGDPRIYGRVYLGTNGRGILYADRQGGATPPPSTSVSPPVPSASVSTPAPATGCSATYKITGSWQGGFQGEVSVRNTGNAATKSWTTGWAFTAGQSLSQVWGATGGQTGTTVSLRDAGWNGALAPNATATIGFLASWTGSNPVPAPITCAATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYU8</accession>
    <name>U5VYU8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative aliphatic sulfonates transport permease protein ssuC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_19305</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362505.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19305"/>
    <dbReference type="EnsemblBacteria" id="AGZ42133">
        <property type="protein sequence ID" value="AGZ42133"/>
        <property type="gene ID" value="AFR_19305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3921"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_3_11"/>
    <dbReference type="OrthoDB" id="1694312at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="63"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="27143" checksum="70664F3FF251B944" modified="2014-01-22" version="1">MTAAERGAPRTALLGLSGLAGLLLLVEVLPRAGIVSDSYLPPASRILAALGREAATGPFWVAVGDTLLAWAIGLAIAVVAGVTVGIVIGAVPVLRALTASTVEFLRPIPSVALIPLAVLLYGSDLGSTLLLVVYAAFWQVLVQVLYGVADVDPVAADTARSFRFSSWGRIRYVLWPTALPYVFTGVRLAASVALVLAVTTELVIGAPGLGAVIAVAQTSGAIPTMYALIVVTGLLGVTINVAARATERHFLSWHQSVRGEASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYV0</accession>
    <name>U5VYV0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18420"/>
    <dbReference type="EnsemblBacteria" id="AGZ41962">
        <property type="protein sequence ID" value="AGZ41962"/>
        <property type="gene ID" value="AFR_18420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3738"/>
    <dbReference type="eggNOG" id="COG1547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125317_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005500">
        <property type="entry name" value="DUF309"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023203">
        <property type="entry name" value="TTHA0068_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34796">
        <property type="entry name" value="PTHR34796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03745">
        <property type="entry name" value="DUF309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140663">
        <property type="entry name" value="SSF140663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16254" checksum="198CD61371D0FE70" modified="2014-01-22" version="1">MSPSRDRDAAGRPRNARPRDGLGRPLPHGAPGIPTTPDDLILPPAEALREAQRLLDAGRPFHAHEVLEGTWKAAPDTERDLWRGLAQLAVGVTHARRGNHTGAARLLQRAALRIEPYAAAAPYGIAVAELAAWARGLASGGPDQDAVPPRLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ02</accession>
    <name>U5VZ02_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13370"/>
    <dbReference type="EnsemblBacteria" id="AGZ40960">
        <property type="protein sequence ID" value="AGZ40960"/>
        <property type="gene ID" value="AFR_13370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2715"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30702" checksum="FBC684DEDE9F3A0D" modified="2014-01-22" version="1">MGLRVVDRVAALGSFTAAADTLGYTQSAVSRQVAAMEAAVGAVLFERHARGVQPTPVGQVLLRHAAVLLAAADAAQRELAAAREQLVGRLVVGGYPTGHVVLLPRALALMRDEHPAVEVAVHSGGSQSLLRMLRARQVELVVVAQMPGIEHDFTGLRSELLLSGRLRVAVPDGHRLAGLASVTPDDLREEPWIVGTGPAGDVHLGAWPGIEQPRIAYAAADWHARLGLVAAGLGIALVPDSVASLISRGMTVVPVDDDGPHTRHVFAVTQRSANPLAKVFVDALRQVATSSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ68</accession>
    <name>U5VZ68_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361084.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13695"/>
    <dbReference type="EnsemblBacteria" id="AGZ41025">
        <property type="protein sequence ID" value="AGZ41025"/>
        <property type="gene ID" value="AFR_13695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2781"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019843_1_1_11"/>
    <dbReference type="OrthoDB" id="1457558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="138"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="29210" checksum="A37CFBD5E2B52313" modified="2014-01-22" version="1">MESITKNRQSPDTLRAMIAKAYGPEQVPAGDDFAEELGHGWFNVAYKLRLRDASHVVLKIAPPPRVEVMTYERGAMATELAALALIREATAVPVPAVDFADRSHELCDADWFVMAFIDADNFGIVRPTLTRAEQADCLDEVTEPRFVEWDLWDSNVMVRDGTIVGIIDHERAYYGDPLIEAGFAATQLPAFGDSTAFLRGYGKTELTSGEQMRRRLYCLHLILIMIIETEYRGHTDTQQYDWARERLTEAMALFGRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W066</accession>
    <name>U5W066_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_20750</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362794.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20750"/>
    <dbReference type="EnsemblBacteria" id="AGZ42422">
        <property type="protein sequence ID" value="AGZ42422"/>
        <property type="gene ID" value="AFR_20750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4207"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="OrthoDB" id="664909at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="329" mass="34654" checksum="D5D3813272D6E734" modified="2014-01-22" version="1">MPGDRRTTLADVAAAAGVSTALVSIVMRDAAGASEATRRRVLEVAERLGYHPDSRARLLRSGRTRLLGVVFGVQHTFHGDLVTGLYAAAEQHGYELALSAVAPGRDERKAIAGLLQDRCEALILLGPQSPAATLAELAARMPVLVVARGVRHPAVDVVRTADAEGLHQAVDHLASLGHHRIAHIDGSRAPGATSRRRGYTQAMAGVGEPRIIPGGLTEEDGARAAESLLTDLPTAVTVFNDRCATGVLDVVRRAGLAVPGDLSVVGYDNSRLARLAHVALTTVAQDVDALTTQAVQRAIERIDGARPTPREIVIPPHLIIRATTGPPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W072</accession>
    <name>U5W072_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15170"/>
    <dbReference type="EnsemblBacteria" id="AGZ41316">
        <property type="protein sequence ID" value="AGZ41316"/>
        <property type="gene ID" value="AFR_15170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3080"/>
    <dbReference type="HOGENOM" id="CLU_1709343_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16328" checksum="3A4FB09E6329147A" modified="2014-01-22" version="1">MINSAADRPPMLEAGASLLAVVGVFSAGRVAYGVVTNLGQNGWSTGARGVFLVLNAIVLIFALFILVLAWQVRRGRTWAWVVSLVLLPFTIFFGGLLLLITALNDMVPFAGIGVVLFSLAALLTLTVPRSVRDHLLRKPAPAMQWAPGHPPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W079</accession>
    <name>U5W079_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_15515</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361444.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15515"/>
    <dbReference type="EnsemblBacteria" id="AGZ41385">
        <property type="protein sequence ID" value="AGZ41385"/>
        <property type="gene ID" value="AFR_15515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3151"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_40_0_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="20727" checksum="B038F6E9C4B7C9AD" modified="2014-01-22" version="1">MSADRGYHHGDLRAALIEASFDQLADGGLGRFSVAAVARRLEVSSAAPYRHFADRDHLLSAVSGAAARNLREAVLAAADAAGSDPAGRLAAAAGAYVRFVTRTGAGFHVIFAEELYQIPDDARREQTRALISTLLDLATEAGAASHDEALLLVEATVAVAHGYVSLFADGFFARNGRTLDSIAARATEAAHALVPTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0D6</accession>
    <name>U5W0D6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amino acid efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362869.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42497">
        <property type="protein sequence ID" value="AGZ42497"/>
        <property type="gene ID" value="AFR_21125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4282"/>
    <dbReference type="eggNOG" id="COG5006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057295_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="141"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="30672" checksum="7621EEB76BC7FDB3" modified="2014-01-22" version="1">MRTETRTGTSMALGAMVLVQLGLAASVGLLDRIGAGGAAWLRLAWAGVILLVLVRPRLRSFTRRTLLGSVALGVVTAGITMLFMEAVARLPLGTASALEFLGPLTVAVVRGRRGTKVWPALAAVGVLLLTEPWHGGIDLAGVGFALAAAVCWAVYILLTQWVGDEVSGLQGLAVSMPVAGLVATLVVGPSVLDGMTWQLVLLGAGLAIILPVVPFTLELLALRRLTTASFGTLMSLEPALALVIGLLLLGQVPGWAPVAGIAFVVAAGIGAERTGARAHPGPDLPRVVEAEGQTAAVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W199</accession>
    <name>U5W199_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361944.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17285"/>
    <dbReference type="EnsemblBacteria" id="AGZ41735">
        <property type="protein sequence ID" value="AGZ41735"/>
        <property type="gene ID" value="AFR_17285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3505"/>
    <dbReference type="eggNOG" id="ENOG5033PCM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340866_0_0_11"/>
    <dbReference type="OrthoDB" id="1488366at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="22515" checksum="50E428485AFC41D0" modified="2014-01-22" version="1">MDLEELAQVIAEVPEIEVSIEGGLVVTRVPAIGDAVRLEPVDVLDWEYLIAPSGVPGIELEVRRGHERLPLIITVDDVVFMPAYAADMVEPDAPLLRVPSSPNLVAYSEMHRDVRALGKAIDDPALELDPETLGATLLVHRCFLAGALRAGLWPVRVAAWWEYMWARVGNDMLAGPFRADPVWDQLMADVTEARRHVTPENVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1M0</accession>
    <name>U5W1M0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Orn/DAP/Arg decarboxylase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_24150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24150"/>
    <dbReference type="EnsemblBacteria" id="AGZ43098">
        <property type="protein sequence ID" value="AGZ43098"/>
        <property type="gene ID" value="AFR_24150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4892"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_1_3_11"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="PTHR11482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="3">
        <location>
            <begin position="4"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="2">
        <location>
            <begin position="238"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="350" mass="37573" checksum="7A1B70D18455B286" modified="2014-01-22" version="1">MRAARIVAALDAFRNAFPQVRPFYATKCNTDPGVLSVLHTTGARFEVASVEEIRILGTLGVRGQDVIFSNPVRAREQTRQASRAGVYRFAVDSREELHRVAEEAPGSCVYARLATGGRASVVPSEGKFGVDVRGAIDLLVRARDLGLVPYGITFHIGSQSLRPAALEAALGDVHTVLSRLVSAGIRLQMVDLGGGFPAAYDRPVPPLAAFGRAAAAGIAQLPYPVEVFAEPGRCIVAEAGTFRCRVIGVAQRPSGYWAHTDLGVFNGMMEVLESGGDLRYPIRDNRGSANRRRFHVTGPTCDGQDTYARDVPLSADLREGDEVLIGSAGAYTTVYASRFNGFARPRVVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W227</accession>
    <name>U5W227_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23895"/>
    <dbReference type="EnsemblBacteria" id="AGZ43047">
        <property type="protein sequence ID" value="AGZ43047"/>
        <property type="gene ID" value="AFR_23895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4840"/>
    <dbReference type="eggNOG" id="ENOG5031SST">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1821235_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="141" mass="14996" checksum="2C573AD5E02CD373" modified="2014-01-22" version="1">MKFFLWVGLPVIATMGFMFGVFDLVPAWQAHNGSGATGTFTAEREDCSRRSCNFVGSWAATDGSSSRTDIILYDEPDAFTTGRTIEALDTGARNGVFATTGGSTYLLVTAFVVGGIAALIGWVFVIRNAFRRRGAAVPVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W274</accession>
    <name>U5W274_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GlcNAc-binding protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18930</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362433.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18930"/>
    <dbReference type="EnsemblBacteria" id="AGZ42060">
        <property type="protein sequence ID" value="AGZ42060"/>
        <property type="gene ID" value="AFR_18930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3845"/>
    <dbReference type="eggNOG" id="COG3397">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063855_0_0_11"/>
    <dbReference type="OrthoDB" id="1005693at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03067">
        <property type="entry name" value="LPMO_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666302" evidence="2">
        <location>
            <begin position="28"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="3">
        <location>
            <begin position="270"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="373" mass="38696" checksum="98D5D55DE13E82F1" modified="2014-01-22" version="1">MRKRFLYAAAVLLATGVGVTVVQTAAEAHGAMMAPGSRTYFCWKDGLSGTNGSIVPKNPACAAAVSQSGTTPLYNWFAVLRSDGGGRTSGFIPDGQLCSGGTGGPYDFTGYNLARNDWPQTHLTAGASMRFDYNNWAKHPGTFSLYITKDSWSPTRPLAWSDLESQPFSQVVNPTSVGGPGTNDGRYSWTANLPSGKSGKHLIYSVWSRSDSQETFYGCSDVTFDGGNGEVTGVGDGTIPPPPTDPTTPPPSGPTSPPASPPTSGPTTPPPAGSTGCSAMYSVTGGWGGGFQGEVMVHAGSTAVKNWTVSWTFPGSQTVTQLWSGKYTASGSLATVKNETWNGAIAPNSHTTFGFIGTGTAPTTVQNLSCTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2E5</accession>
    <name>U5W2E5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Macrolide export ATP-binding/permease protein macB 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_25720</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364081.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25720"/>
    <dbReference type="EnsemblBacteria" id="AGZ43408">
        <property type="protein sequence ID" value="AGZ43408"/>
        <property type="gene ID" value="AFR_25720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5214"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012341_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="497"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="725"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="782"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="815"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="19"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="293"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="757"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="854" mass="86727" checksum="F0005FB3B3D0B7A3" modified="2014-01-22" version="1">MSVLQTQLAGVARRPTRLLLTGLAMLVASFVVYATVLAQQITVRTMLDGLSGTPASVDLVVGGFAPGEVGNAPYVTTAELAKIKAVSGVAAAEGRVEASGETGGQYLSLSADPGSGPLAVTHVTQGRYPKAPGEIAVSPRTADRMGLPVGTDITLFTSWDDNGKPTKPVKFSVTGLVDARDDYGFTGYAPQSTISALVPSGNLSQIDLSLKAGADAAVVQSAVDRIMQSAPVPADYQPAQVRTGLEVRTAEANNAASDLNALFAVVAMFVGIAVVAAGLVATSTFRIVFAQRMRQLALLRAVGAGRGSITRALAAEGALTGVVAGVVGVLGALALGHALPPILRGFDLSVSSPGIPVLPAIGVILLSVFITVLAVLAPAFSASKVAPLEALRSASTTGGRRDIGKARWVFGLLLVLGAAASAAYVITNLPGRDPRDYDPAPMLLMVVASGALAFFALMALGPVLVRPVLATVGWPLRRFGSVGRLAVGGVGGAPRRAAAVSVVVALGVTLIAGVVVTGASVRILADRELASSAPADFELTANAGEPMPAAVYDQAKASPDLTHVTPYRRLADIQLGNAEGKVEATDVALRQLTTSDKIDVSAGSLTDIGPGAIAISGFTAEVTGLKFGDTTTMKAGKKTTQVRVVATLPDSAPLNSYVLADPSDLTKLGAPAEYSGLLADAAKPGEDGRTAGQKVLQQLVQGRTGLGVEVLADQRDQLDTILNGVLAVALGLIGLTVLIAIVGVGTTTALSVVERVRESGLLRAVGLSRGGLRAMLTAESSLYGVIGATIGLVLGVPYAWLSIKALGVNAPLSLPVWQLLAVFAALVAFTALAGVLPARKASRVSPVAALATDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2L3</accession>
    <name>U5W2L3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363842.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43252">
        <property type="protein sequence ID" value="AGZ43252"/>
        <property type="gene ID" value="AFR_24930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5050"/>
    <dbReference type="eggNOG" id="ENOG5032K7Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_999765_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="30448" checksum="F9CEFFBF68156BDE" modified="2014-01-22" version="1">MTSVLAAEPFWQRFLTSAGFGGLMALTAALIAARIATQQLRHTKSQQANERWWTTLTWVYDRAVVEKDKRVALPHHVTFAMLTQLAERAQQPPADRLQQSSIKSILAMFEATDDGTAPQQGTIEVSDPAAAALLDDLRTTLSNDEELRERAEQLRYLESARTAVEREAAALGADVSGQNATVAVSWRGREVLVQIRYAREPIPTLTLLQAVDRLQRDVAAGHTTIGGILVVTALSRPAIDAFLAARNSTGIEVVAWTGSPDDRLLHEALQRLRPAPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2M3</accession>
    <name>U5W2M3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364218.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26070"/>
    <dbReference type="EnsemblBacteria" id="AGZ43478">
        <property type="protein sequence ID" value="AGZ43478"/>
        <property type="gene ID" value="AFR_26070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5284"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_336464_0_0_11"/>
    <dbReference type="OrthoDB" id="514542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="608"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="790" mass="83751" checksum="425945FEC53FBF05" modified="2014-01-22" version="1">MRAAGLAVKRFFSKAKPDDLLLLAITGHGKRDDRGKLFFVNRDTVPDSLWVTGLSAEHVSHEMRNSPARKTVVLLDCCFSGAFVHDEGLAIPLPPAAPGQAATEEDEEDRRTRGTVIITASTSIEQAFETNGDGDKIRSGLFTRCVVDGLETGEADLNRDGEVDVNELYQYVATQMGRIAKGRRQNPTYSAHRMQGVLRIAHSALPQVVDRPQPVRTPRPRPAPVPTNHGPLSRRVLVPLLVLGGLLSGCATQADSTIGGGGCPTPVQVRVAAAPGGLTAYREIAAGFEDWVAGRQHGCRGVDLYVYPVEAGEVTAGLRRGWTTGEDGADYLRDAGPQPDVWLPAAATDVPPGGLRDVIDRVEQVAQTPVVLGVPARARADDGLRRAVLSWPDLFAAVSRDPGVVRADFASSVIARMATAKLYADGTIDQAVARTTVEQPLEKALDAGTYPVGDEAGLLCRQRAARSSTAVIVTEQQLVRFNRGDPLDGACTGAAPPASADRLLAFYPADTPALEQVVVTLDWQGRAQSATTRAYATWFVRWLRQIPGRQVLLRAGLRPPGLDADEPLGPAYGALTVWPFARVVPDEPDELTRSDTATLHAATRRPGRFLVALDASGSMRTVTPDPDRTRFEVAAAAVEQAATRLGRRDELGLLTFSGRATREVLPIAPAGADPVGRVHRATAPIQPAGGTPLYEAVRRGASALRAGPGDPLRTLVVLTDGQDTSGQPRPSAAQTAGVRIFVIAVGDVSCADATLAGLATGTGGRCFDAGKGSLEPVLTGLFRAAWETGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2X7</accession>
    <name>U5W2X7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-arabinose isomerase</fullName>
            <ecNumber evidence="6">5.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">araA</name>
        <name evidence="8" type="ORF">AFR_25540</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of L-arabinose to L-ribulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>beta-L-arabinopyranose = L-ribulose</text>
            <dbReference type="Rhea" id="RHEA:14821"/>
            <dbReference type="ChEBI" id="CHEBI:16880"/>
            <dbReference type="ChEBI" id="CHEBI:40886"/>
            <dbReference type="EC" id="5.3.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate degradation; L-arabinose degradation via L-ribulose; D-xylulose 5-phosphate from L-arabinose (bacterial route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the arabinose isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.4" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364010.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25540"/>
    <dbReference type="EnsemblBacteria" id="AGZ43372">
        <property type="protein sequence ID" value="AGZ43372"/>
        <property type="gene ID" value="AFR_25540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5175"/>
    <dbReference type="eggNOG" id="COG2160">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045663_0_0_11"/>
    <dbReference type="OrthoDB" id="507566at2"/>
    <dbReference type="UniPathway" id="UPA00145">
        <property type="reaction ID" value="UER00565"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008733">
        <property type="term" value="F:L-arabinose isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019569">
        <property type="term" value="P:L-arabinose catabolic process to xylulose 5-phosphate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00519">
        <property type="entry name" value="Arabinose_Isome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024664">
        <property type="entry name" value="Ara_Isoase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038583">
        <property type="entry name" value="AraA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004216">
        <property type="entry name" value="Fuc/Ara_isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009015">
        <property type="entry name" value="Fucose_isomerase_N/cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003762">
        <property type="entry name" value="Lara_isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38464">
        <property type="entry name" value="PTHR38464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11762">
        <property type="entry name" value="Arabinose_Iso_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02610">
        <property type="entry name" value="Arabinose_Isome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001478">
        <property type="entry name" value="L-ara_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50443">
        <property type="entry name" value="SSF50443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53743">
        <property type="entry name" value="SSF53743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0054">Arabinose catabolism</keyword>
    <keyword evidence="5 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arabinose_Iso_C" evidence="7">
        <location>
            <begin position="358"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="496" mass="53754" checksum="ADAB2455AD19117B" modified="2014-01-22" version="1">MKPQIWFLTGSQHLYGPETLDQVASQSQQIQQLLTSSGGISAEIVSKPVLTDSSAIRTVMLEANADPSCLGVIAWMHTFSPAKMWITGLDALRKPLLHLHTQLNQTLPWASIDMDFMNLNQAAHGDREFGYIQTRIGVARKTIAGHASDPSVVTRIDGWARAAAGVQHLRGLRLARFGDNMRDVAVTEGDKVEAELKFGVSVNTYGVNDLVAVVDATPDSEVDALVAEYADVYRLDPQLARGGDRHESLRYAARIEAGLRTFLTDGGFGAFTTNFEDLGGLRQLPGLAVQRLMADGYGFGGEGDWKTSALLAAVKAMGTGTGRGTSFMEDYTYHFGPGEQKILGAHMLEVCPSIAADKPSCEIHPLGIGGREDPVRLVFDAAPGAGVTIGMADLGDRFRLVANVIEVTPPDEPLPKLPVARAVWKPAPSLATSAESWLTAGGPHHTVLTQAVGVEPLRDFATMLHTELLLIDENTTTHDFADRVRWNQAYYRQFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W390</accession>
    <name>U5W390_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20645</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362773.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20645"/>
    <dbReference type="EnsemblBacteria" id="AGZ42401">
        <property type="protein sequence ID" value="AGZ42401"/>
        <property type="gene ID" value="AFR_20645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4186"/>
    <dbReference type="eggNOG" id="ENOG50332K7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080168_2_0_11"/>
    <dbReference type="OrthoDB" id="1759242at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043231">
        <property type="term" value="C:intracellular membrane-bounded organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="23352" checksum="A1AE98E15EC51BB7" modified="2014-01-22" version="1">MATSQLPLADDLYLAAHDNARGRCLLSPATLGLGLAAGLLAELVLWRRLEIQDAGLTVVDERPTAEAATSAVLEQLLREGHHRQVRDWIAFLATGVATELVERRLARAGLVKRHEKRGLLGTKVRFVPTDLTVAGWPGTRIRIAATRGELLDSPDLVLTGLVLATGLDQHVLVTLEAAEREHLFGQLRRRLPVMLQHLVGQAEAAVGDAVMARRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W398</accession>
    <name>U5W398_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">1,4-alpha-glucan branching enzyme GlgB</fullName>
            <ecNumber evidence="9">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Alpha-(1-&gt;4)-glucan branching enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glycogen branching enzyme</fullName>
            <shortName evidence="9">BE</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glgB</name>
        <name evidence="12" type="ORF">AFR_27110</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27110"/>
    <dbReference type="EnsemblBacteria" id="AGZ43683">
        <property type="protein sequence ID" value="AGZ43683"/>
        <property type="gene ID" value="AFR_27110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5494"/>
    <dbReference type="eggNOG" id="COG0296">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004245_3_2_11"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102752">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02855">
        <property type="entry name" value="E_set_GBE_prok_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00685">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006407">
        <property type="entry name" value="GlgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044143">
        <property type="entry name" value="GlgB_N_E_set_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="PTHR43651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01515">
        <property type="entry name" value="branching_enzym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 9" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="5 9 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="11">
        <location>
            <begin position="247"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9 10">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9 10">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ43683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="720" mass="80712" checksum="78EB01A09976B375" modified="2014-01-22" version="1">MIGQTTPPTPAVTGDQRALNAVVSGDTHDPHGVLGTHPSGDKTVIRTMRKGAKDVFVVVDGEKTPMAQVHPDGIFAVELPGTVLDYRLDVDGTVCDDPYRHLPSLGELDLHLIGEGRHEKLWKVLGAQPRDGGVAFAVWAPSARGVQVIGDVVGWGPFDGWPMRSLGSSGVWELYVPGASSGTKYKFKILGRDGVWREKADPLAQHTEIPPRTASVVFASSYEWQDADWMAERAKKQWHQEPMSAYEVHLGSWRPGLSYVELAEQLVDYVVETGFTHVELMPVMEHPFGGSWGYQVTGYFAPTSRFGSPDEFRYLVDKLHQAGIGVILDWVPAHFPKDEWALARFDGTPLYEHGDWRRGEHPDWGTYVFDFGRREVRNFLVANANYWCEEFHADGLRVDAVASMLYLDYSRKEGEWTPNQYGGRENLDAISFLQEMNATVYRNNPGVVTIAEESTAWPGVTRPTHLGGLGFGFKWNMGWMNDTLSYMEKEPIYRQWHHHQMTFATVYAWSENYILPISHDEVVHGKGSLTGKMPGDDWQKLANTRALFGFMWAFTGKQLIFMGTEMGDRQEWSEERGLDWDLLADDSRGGGIHKLIQDLNRVYKESPALWTQDTTASGFRWITSEDSQHNTFSFLRFAPNGDTLACIVNFAAVPHENYRIGLPRPGVWREVVNTDSSLYGGSGVGNLGEVHAEEMPWHGLNASASLRVPPLGAVWLKLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3R3</accession>
    <name>U5W3R3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21380"/>
    <dbReference type="EnsemblBacteria" id="AGZ42546">
        <property type="protein sequence ID" value="AGZ42546"/>
        <property type="gene ID" value="AFR_21380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4337"/>
    <dbReference type="eggNOG" id="COG0699">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031445_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="438" mass="46608" checksum="700EAED99F8DBEB9" modified="2014-01-22" version="1">MAPTAVGECTRVVTRFRYGPADRVDVVRRDGTRSSLPLGDDGMIPQRLGVGAAQVAYVDVMLTSEKLRDLTVVDTPGLASTDTQVSARAAAELGETTAPFDADIDADSASEVAAAEAVVYVFTQAVRADDVRALEAFRTSSARLASSPINALGVFGKIDTLVGGAGDPWPVAGPLAEQQAALLARTVSEVVPVVGLLAETGEAGRLTAADCAALKELSVLPAAELRVLLASVDLFRTRPGPVGAQRRERLLGLLDLYGIGFALAQLGADPQLGTGELVRRLVHASGFPRLRQTLDQTLRWRADAIKAGWALARLERVAGHESDPRDREALRGATERLLRDPAYHRLRLLEVAQRVATGAVPLPVAWEQELTRLATSEDARWILRLPHATAPELAAAAVEAANRWRVYAVAGAGPAQSRVAQVAHRGFHLLAQAVREQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W426</accession>
    <name>U5W426_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cyclase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363032.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42661">
        <property type="protein sequence ID" value="AGZ42661"/>
        <property type="gene ID" value="AFR_21955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4451"/>
    <dbReference type="eggNOG" id="COG1878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030671_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="SSF102198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="25226" checksum="350D47C3AD6FDA5A" modified="2014-01-22" version="1">MLNLVSLSHVNDPATTIVYPGDPPFELETVATIGDDGFFLQYVRVGEHTGTHWGAPGHFTAGAALADDLEPADLCRPAVKIDVREQCARDADFELSVQDLQDWERRHGRIPAESVVVLWTGWDSRWGTEAYLNRDEDGVVHQPGFSLEAVEWLTATGRLGHRGGTGTDTFGPDPGRDTTYAVSRLVYQRHRISLEVLADLGALPATGAHILCGGQINKGGSGSTALIYGVLPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W467</accession>
    <name>U5W467_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363077.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22180"/>
    <dbReference type="EnsemblBacteria" id="AGZ42706">
        <property type="protein sequence ID" value="AGZ42706"/>
        <property type="gene ID" value="AFR_22180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4496"/>
    <dbReference type="HOGENOM" id="CLU_2165539_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="110" mass="12218" checksum="E8836942875B039A" modified="2014-01-22" version="1">MRKLLDEIDGGRFEISVTGLSISPGDGALSCSLLARSSSPAFFSWLRRELRANWEAAAPGDEGAPIEFVRIDDQLIDEWAVQGRLDPSSIMAIDLARKALNRRDDDQRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W470</accession>
    <name>U5W470_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28340"/>
    <dbReference type="EnsemblBacteria" id="AGZ43929">
        <property type="protein sequence ID" value="AGZ43929"/>
        <property type="gene ID" value="AFR_28340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5745"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_306675_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="840"/>
            <end position="906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="909" mass="97252" checksum="77CE539C9F3D8EB9" modified="2014-01-22" version="1">MAAAGEGCGGVLFVVGEAGIGKSRLAAEAARLATGAGQCVVRGRATTSSAQYRALREAFMSTLRRTGPPGDPQLVPYRAALSRLVPEWQLTRPPGVDDAPVVLAEAVLRLLVSLGGGHGCVAVLEDLHDADADTLAVVDYLVDNLGQEPVLVVATVRSGPGAGADLVRAAGRRRAATVLELDRLDDGEVRELAALCLEVPGDRVPEPILTRLLEVADGVPFHVEELLADGGNGVPRTLSEGVAARADQLGPQGRALVQAAALLGRRFAATVAGEAAGVTGDDLATCLRLAADAQFVRPGSEPDVYVFRHALTAEALRGGMIPAEAARLARRAAQVIGERDEDLAAELWCRAGDLRRAAGSRSRLARRALAEGAVSTAIVQAERGLELVKDQSAGEPVLGELREVLLDALIDAGEVERAVELGTWLDLHTTTARRATVHLRLARAAAAAGQWAEGLRQIAGVRRLAGPDAEVDAVEAWLTFLRPAPDRMLRARQLAGRALENGRPEVLCAALEVLASCARTTDLEASRVWCDRALAVAEDHGLTTWRVRFLFHLGVLDAIHDADPARLILAHDTAVASGAVVTALVIGCELAVVHLTRGDHDAAERCAQAAETAAARLRLAETRLIALALRVCVAAHRGSREQTEVLYDRYRALGGDDIDHAAAVQGLGLAFCSLLEDNPERAWQDLDRAIAREDNHPPHYLSFNRGPYLLLATLAGKAGRAEHDELATSAHGQARWNRQFVTLSRAVLDGCEGNGDEAVAAVAEFEELATPYPLAHHLGLRLIGERAHADGWGRPRDWLTAAETYFHRTDSVRLAAACRTQLRDMGVPVAQRRRGSDRIPPALRRMGVTVREYEVLELLVDRLGNKEIAGRLFLSPRTVEKHVASLLLKTGHDDRTGLARFAVRERESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W634</accession>
    <name>U5W634_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YceI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31890"/>
    <dbReference type="EnsemblBacteria" id="AGZ44638">
        <property type="protein sequence ID" value="AGZ44638"/>
        <property type="gene ID" value="AFR_31890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6455"/>
    <dbReference type="eggNOG" id="COG2353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071003_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007372">
        <property type="entry name" value="Lipid/polyisoprenoid-bd_YceI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036761">
        <property type="entry name" value="TTHA0802/YceI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04264">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00867">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101874">
        <property type="entry name" value="SSF101874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YceI" evidence="1">
        <location>
            <begin position="9"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="18015" checksum="EF482246F061B200" modified="2014-01-22" version="1">MTTTARPGTYRIDPRRTTVHLTATHFLGLKPVDATLTVRSGTLMIAADPEHSTVSAELDATSFTTDDKRRDKDVLGHRFLATDSHPVISFRSTAVTRTPEGWQLTGILALRGGSAPINLHLTEAVPVADGYRFTATTTIDRIAAGVTAGRGLIARHVKVRIECVATAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W647</accession>
    <name>U5W647_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31645"/>
    <dbReference type="EnsemblBacteria" id="AGZ44589">
        <property type="protein sequence ID" value="AGZ44589"/>
        <property type="gene ID" value="AFR_31645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6405"/>
    <dbReference type="eggNOG" id="ENOG502ZF26">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066207_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024787">
        <property type="entry name" value="EcsC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12787">
        <property type="entry name" value="EcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21154" checksum="0972E4898453BDF0" modified="2014-01-22" version="1">MKADPQYAPEHLALEAVRRLGPEAQRWAAQARADQPGVTPDALAQQAVKRFTNHARLSGAVSGATGLPGAVVDVGVLAWTQSRMVLHMAAAYEVDPTAPERAADLLVLQRVHKAAEAARLALGVAAGRERAARLFAGSSGRPLTGVMLKLGVKLAQMAGVRAAKRMFAKVIPGAAVVLGTWANSAATKDLARRTTEHFART</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6A1</accession>
    <name>U5W6A1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561050.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32300"/>
    <dbReference type="EnsemblBacteria" id="AGZ44718">
        <property type="protein sequence ID" value="AGZ44718"/>
        <property type="gene ID" value="AFR_32300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6538"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_27_3_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="175" mass="19561" checksum="A1C251743A97D2A4" modified="2014-01-22" version="1">MGLFGRQGYHATTIAQIEAAAGLSAGAGGLYRHFKSKRALLEEGLRRQFEAGAPLLASLEQTADLPRRERILAVARAGLRRLDQERDVNRLLLRDLATFPDLLDQVRDHELARVHRALTSWVERESPDHPDPAALASVLMSALSHYWVLTDVYAGRHPHGVDEDRFLRTLADLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6C0</accession>
    <name>U5W6C0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_26195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364263.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26195"/>
    <dbReference type="EnsemblBacteria" id="AGZ43501">
        <property type="protein sequence ID" value="AGZ43501"/>
        <property type="gene ID" value="AFR_26195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5309"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="143"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="22857" checksum="1C95FEB6AAA48512" modified="2014-01-22" version="1">MRVVIAEDGLIVREGIAGMLRHFGHEVVAAVADAPALLAAVAEHRPDVVVTDVRMPPGFSDEGLQAAIALRAADPDLPVLVLSQYVEQTYAAELLDSGRSAGVGYLLKDRIGDVSEFVDALVRVAAGRTVVDPEVVRQLLGRRRDPVQRLTPREREVLSLMAEGRSNTAIARALGVTEAAVAKHIGSLLAKLDLPPDADDHRRVRAVLAYLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6T9</accession>
    <name>U5W6T9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560033.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27170"/>
    <dbReference type="EnsemblBacteria" id="AGZ43695">
        <property type="protein sequence ID" value="AGZ43695"/>
        <property type="gene ID" value="AFR_27170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5506"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076586_0_0_11"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="33561" checksum="E7B183049D17A78B" modified="2014-01-22" version="1">MTPTQLRAYSAVVRLGSVKQAAAQLEVSESAVSLNIGQLRRELGDQLFTRTAAGLAFTPGGLRLASRAAELLGLQDRTVLEVSAAGRGRRLLRVAASSLFAEHAAPGLIELFAKRAADLDVELSVRNPAHFDTLLLTRTVDIAIGPQPAAVEPGLASRPVMNYRVVLVVGPTHPLAGVSAGPHQLREQTWLLGPSAAADVGAVPALLRRLGVPEENQQIFQSHAAALEEAKRGKGVAAGLSFTVAQDVAHGHLLHVTGPQATFETVWHSLMLTEQGSPSPAAELSRFAATPRAIQAMMRGAGVTVGHFRPSVHVTLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6U5</accession>
    <name>U5W6U5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">7,8-dihydro-8-oxoguanine-triphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561059.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32345"/>
    <dbReference type="EnsemblBacteria" id="AGZ44727">
        <property type="protein sequence ID" value="AGZ44727"/>
        <property type="gene ID" value="AFR_32345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6546"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_3_3_11"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="27228" checksum="D45BB2A7019697E9" modified="2014-01-22" version="1">MTDEREFLESYDPAEFAPVSVTVDVVALTIRDGQLHVLLVERGGHPFAGWRALPGGFVQDEALDAAALRELAEETGLRPGEGDLDRVHLEQLRSYGDPGRDPRMRVISVAYLAFAPSLPDPRAGGDASGAFWVPVDEVGELAFDHTTILADGLDRARSKLEYTPLATAFVGDEFTISELRAVYAAVWDEELHAGNFHRKVLSVPGFVESTGETSTRGGERGGPKARLYRAGDARLLHPALLRPAREDGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W733</accession>
    <name>U5W733_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561345.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33750"/>
    <dbReference type="EnsemblBacteria" id="AGZ45008">
        <property type="protein sequence ID" value="AGZ45008"/>
        <property type="gene ID" value="AFR_33750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6832"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102962_0_0_11"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="22"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="20819" checksum="D0AB51D14CF308E8" modified="2014-01-22" version="1">MSSNQEFPDYDLPDTVSVSTPAQLRAIADPLRTTVLDLVLERAATVAELSAAVGRPKSTVAHHVNVLLEAGLLRVVRTRRVRAIDERYYGRTGRTIHVGVVRRPGETETPVCVNGLSVAAAESVPAHEDDRLYTTLRHVRIAREDAAAFWQRVEELVRDFTQLPRSGDTVYGLVAGLYPTEHPTLPESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7A0</accession>
    <name>U5W7A0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560163.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27825"/>
    <dbReference type="EnsemblBacteria" id="AGZ43826">
        <property type="protein sequence ID" value="AGZ43826"/>
        <property type="gene ID" value="AFR_27825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5639"/>
    <dbReference type="eggNOG" id="ENOG5033HDJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1102309_0_0_11"/>
    <dbReference type="OrthoDB" id="1874682at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="27701" checksum="E9653765DA17607F" modified="2014-01-22" version="1">MFAFATAVLALLALGTAAMVIIRVAWPAPSPSPLIFRATTGWRLAGIVAGAAVAVLVARSGDLGRGFLVAGPAFGLTVFAGALIGELTRPGPDGEIRRATLRVRRARDYVPRVLGGVVVVSTVLLAAIATVTTAAGSADDLGRAGRALSCPGLLQTPWPGSYYTLPALALVAAGLAVAAYALHRVARGPQTDSSRRRSAEVITAAAGILVLLPLAGISLTAGVLLGSTDLTCNTPWWEPAGRSLMVLSLLAFGTAVWCGACLLTPSRRSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7B9</accession>
    <name>U5W7B9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561400.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34025"/>
    <dbReference type="EnsemblBacteria" id="AGZ45063">
        <property type="protein sequence ID" value="AGZ45063"/>
        <property type="gene ID" value="AFR_34025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6887"/>
    <dbReference type="eggNOG" id="COG4552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050659_1_1_11"/>
    <dbReference type="OrthoDB" id="1497065at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SSF55718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="396" mass="43262" checksum="68E6607D440693BC" modified="2014-01-22" version="1">MEIRQVSAAERTDTMFPLQTYAFMPSPWPEADREVYAGRMRFYETAVSLVAEEGGQALAGVAAFPMRQNARGAVHDMAGVASVSTHPAARRRGFVRELLWRLLHQMRDQGCTVSALYPFRPSFYGRFGFVGLPRVRTATFAPEGLSHLLRADLPGEVQRLPMAECFDDFNALTLRLLGERHGFSVYDEVRTAEFRESPARWVAVARSGGEVVGAVAYRIDQYGGDLIADDLLVTGSLGRALLLQFFARHVDQVSRVKLTIGTDEVPELWGTDFAVLTEGRVEHPRKGGPMARLLSLEALTGTSTAAKDEAVVVEVVDDEFIGGRYQLSADAGRLVVEPSKAEPQATLTCAGISALAYGVLDPIEVLTRGFGQVDAAAAQSLAALFPKQMPYLFADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Q4</accession>
    <name>U5W7Q4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Polyphosphate glucokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561564.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34860"/>
    <dbReference type="EnsemblBacteria" id="AGZ45228">
        <property type="protein sequence ID" value="AGZ45228"/>
        <property type="gene ID" value="AFR_34860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7053"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065796_1_0_11"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="27162" checksum="9AAE2F874F68E2EC" modified="2014-01-22" version="1">MAFTLTIDCGGGGIKGSVLDDAGTMRAQPLRVPTPYPLPPDLFVKTLLELGERLPTADRVTVGMPGMLRHGVVVATPHYVTRSGPRTKIDPELVEAWHGFDARTALSEAFGLPTLVLNDAEVHGAGVVAGTGCELMLTLGTGLGCALFDGGQLAPHLEMSQAPVRWGMSYDTYIGEHERRRLGDALWSRRVRNVVEGLRPVFLWDRIYLGGGNSRLITPTQLARMGDDVVVVPNTAGIVGGVRAWTLRADGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W858</accession>
    <name>U5W858_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_35365</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561665.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35365"/>
    <dbReference type="EnsemblBacteria" id="AGZ45329">
        <property type="protein sequence ID" value="AGZ45329"/>
        <property type="gene ID" value="AFR_35365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7156"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_91_5_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901564">
        <property type="term" value="P:organonitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006189">
        <property type="entry name" value="CHASE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042240">
        <property type="entry name" value="CHASE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03924">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666424" evidence="5">
        <location>
            <begin position="40"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="7">
        <location>
            <begin position="397"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="6">
        <location>
            <begin position="539"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="794" mass="85389" checksum="DE960E7CAB6E1363" modified="2014-01-22" version="1">MRSTSLGSNRRRWSGVALVVVVALLGTAVAWVATGNARRAEQRYAAQLMDRYTSDLTRAITTEIQRYGDTLDDVATALGAQTDLTADDFGWITNKISNRRLPGATSLGFVVSTPDSGVGALQSYWRERGASNLMLSPVDTAGEHAFAVFTRSFDGVPVVAGMDLSAAPEASDALQDAQFLGGFAVSRAYVLLKDRALPVTQQQLSFLFAVPVYRIGGAFRGWLTMGVHGRDLLTETLRTQSHGAVTAGLEDPTDRVERTIASASGGKARNYPAALDRTASVSAGLRTWRLEVHPTDQLLEETDRGIVGTTFAVAMTITLLLTLLVALLAGARNRAMSKVDAATAALRSDIERRQQVETRLRERELELEQMALHDPLTGLANRAGLDARLAEVVGTRSDVALLLIDLDDFKLVNDAYGHAAGDAMLTEFAQVLLAGVRTGDVAARIGGDEFVVLITDVPDDARAVAAAQRVLAAAASAPVRLGDDTLPVRASIGVATSRAGDSPKELLRRADIAMYQAKHLGTHGVQLHDPSMTDRRKADSLLAEDLAGALDRDELHVLYQPLVDLTDSRPIGVEALIRWRHPRLGLVSPADFIPIAERTGTITAIGLWVLETACREITRWDGLYVSVNLSPRQLQEPTLVQDVLGVLARTGLAPDRLVLEITESALVDESAGIAMLQEFREHGIRVAIDDFGTGYSSLHYLTRLPVDILKIDRSFVAELNGTPEGSGITEAILRLSQVLHLTAIAEGIETAEQAAELQLLGCGVGQGYLFARPLPPTELQDLLLSPSPRPSSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8V2</accession>
    <name>U5W8V2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">von willebrand factor type a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561949.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36785"/>
    <dbReference type="EnsemblBacteria" id="AGZ45613">
        <property type="protein sequence ID" value="AGZ45613"/>
        <property type="gene ID" value="AFR_36785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7441"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024570_2_1_11"/>
    <dbReference type="OrthoDB" id="736429at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024163">
        <property type="entry name" value="Aerotolerance_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07584">
        <property type="entry name" value="BatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="87"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="33686" checksum="4B7DEE0A180B01AC" modified="2014-01-22" version="1">MTWLSPERLWLLVAVAALAVAYVVMQRRRSRYAVRFTNLKLLDRVAPKRPQWRRHVPAGLFLAMLALLVVGFAQPQAEVQVPRERATVLVAVDVSRSMLATDVDPDRLTAAKAAAREFVANLPPEFNVGLVGFAGTASVFVPPVTDRAAVDAGIDRLSEGTAGRAGTAIGDAIATSLDAIRTLDAEAGEDVPPARVVVLSDGANTAGQDPADAATLATELGVPVDAISFGTANGTIGGSALGGGTGGGGQPVPVDGQTLQAVADATGGNYFEAGSADELRAAYADIGSSVGYETETQDISARFIGFGLALAVLAALASMFWFARLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Y2</accession>
    <name>U5W8Y2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Urea ABC transporter ATP-binding protein UrtE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_30975</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560790.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30975"/>
    <dbReference type="EnsemblBacteria" id="AGZ44455">
        <property type="protein sequence ID" value="AGZ44455"/>
        <property type="gene ID" value="AFR_30975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6270"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017780">
        <property type="entry name" value="ABC_transptr_urea_ATP-bd_UrtE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03410">
        <property type="entry name" value="urea_trans_UrtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="24988" checksum="8CAC9523C7A28464" modified="2014-01-22" version="1">MLEVRDLHVSYGRAQVLFGVGLEAPAASLVCLMGRNGVGKTTLLKAVMGVLPARSGTVTFDGRDITKLKVHERVRLGLGYVPQGHETFPQLTVAENLQVARETAGNRRAGVVDEAFDLFPALRGLLKRRAGFLSGGQQQQLAIARALVTEPRMLLLDEPTEGIQPSIVLEIEEAIRQLHAAGLGILLVEQYLELALRLADRFVILDAGEVVRSGPADDLRDESVRRLLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9E9</accession>
    <name>U5W9E9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562173.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37920"/>
    <dbReference type="EnsemblBacteria" id="AGZ45838">
        <property type="protein sequence ID" value="AGZ45838"/>
        <property type="gene ID" value="AFR_37920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7669"/>
    <dbReference type="eggNOG" id="ENOG5031T4H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_955222_0_0_11"/>
    <dbReference type="OrthoDB" id="2140586at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="29751" checksum="A0339AE4E7D621E0" modified="2014-01-22" version="1">MSDFDEVLERLLTDPSFQAQLANDAVDALAGYQLEASEVELLRQQVSPDSGASAALVEDRVSKSSTFGLFSSFEGLGGVTDAVGSGVAGQGPAPTGSGLGPAPVGGGLGEWAEFGSAAGHVAQTASGQPATEGFGSAPDGWGAGGGEMAARTGFGSAPGVDDAPDLDGLPPVARLGDAPRTGLGDAAQEVGEALGGPGRDDHLAPPKGYDNQVDADGDGTFDKATYRGREDGGAEIRVDLNRDGEADFVGVDKDLDNRVDFADYDKDHDGVFEKRMFDDDGDGWLDRTVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9N8</accession>
    <name>U5W9N8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative signal peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38025"/>
    <dbReference type="EnsemblBacteria" id="AGZ45859">
        <property type="protein sequence ID" value="AGZ45859"/>
        <property type="gene ID" value="AFR_38025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7692"/>
    <dbReference type="eggNOG" id="COG2932">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028723_11_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S24" evidence="1">
        <location>
            <begin position="3"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="98" mass="10956" checksum="F305B2EB1BBF9907" modified="2014-01-22" version="1">MPLFAVLVQGPSMVPALRSGDALLVRRSGRVRAGDVVVARFRSRPDLLVVKRALHEHDGGWWVQGDNEFVTDDSRAYGVADVIGRVVFRYWPRPGRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9T1</accession>
    <name>U5W9T1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Oxidored_molyb domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561067.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32385"/>
    <dbReference type="EnsemblBacteria" id="AGZ44735">
        <property type="protein sequence ID" value="AGZ44735"/>
        <property type="gene ID" value="AFR_32385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6554"/>
    <dbReference type="eggNOG" id="COG2041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094953_0_0_11"/>
    <dbReference type="OrthoDB" id="1729032at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="SSF56524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_molyb" evidence="2">
        <location>
            <begin position="36"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="199" mass="22791" checksum="C3BC4BCE35DA43DD" modified="2014-01-22" version="1">MSIISPGFGGRRRSSRPDLPPGQYLTRDFPVLSAGPTPRIPLDRWQFTITDEVGKKHSWSWKEFQELPAEDVTVDIHCVTKWSKLGTTWRGVALDTLFENIETTADYTMVHGYGGYTTNVPLEDLLDGKAWVAYKFDGEDLAPEHGGPARLVVPHLYFWKSAKWVNGIEMLQQDEPGFWEEAGYHIYGDPWREQRYQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9V8</accession>
    <name>U5W9V8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38670"/>
    <dbReference type="EnsemblBacteria" id="AGZ45988">
        <property type="protein sequence ID" value="AGZ45988"/>
        <property type="gene ID" value="AFR_38670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7824"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1159161_0_0_11"/>
    <dbReference type="OrthoDB" id="1117015at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="252" mass="27854" checksum="75B0B8768AC8D764" modified="2014-01-22" version="1">MDAVTAKVAILGASSPLGLGVRGRSYGVRVGESLGPGAEILQLSRSALTVADVEPSTLEQIREFAPDLIILSFGAAEAYVHPSRFLQALLDRFAPQSWRGPGGMEPRPYYSNARLKRLRQCLVSRAKVQVKRLIIACTGGFHRLPSAEFAARLREVLDQLGPAQKVLVGLWRVDDYMFPRSNPLLARNNALARDIAAERDDVLFISTADEVRYWDDFLADHAHLNDHGHDRIAEMILEATRSDRLTGTKPPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA79</accession>
    <name>U5WA79_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type 11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39145"/>
    <dbReference type="EnsemblBacteria" id="AGZ46083">
        <property type="protein sequence ID" value="AGZ46083"/>
        <property type="gene ID" value="AFR_39145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7922"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061789_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="47"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="29785" checksum="14826736F8AA195A" modified="2014-01-22" version="1">MDMDPRVRRRYEVYDEDQRLRRPGLGDLVRLRTWDIFDRYVPTGVRVADIGGGPGIHADRLARGDREVLLFDPMPGHVEIARGRGTFQAEIAEARQVPLDDASVDVVLLMGPLYHLVEPEDRLAALREAARVLRPGGLILAEIITRYAWVMDATGKGLLGEPGTWDDFDWIARTGVSKDPDQVVDGSFFAYFHRPEELRAELTQAGFEDVELLAVEGFAGLFGDLAERMREPGDLLRALRITEAEPSMLGASAHVLGLARSCPNPASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAB0</accession>
    <name>U5WAB0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_39345</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39345"/>
    <dbReference type="EnsemblBacteria" id="AGZ46123">
        <property type="protein sequence ID" value="AGZ46123"/>
        <property type="gene ID" value="AFR_39345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7960"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="909"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="254"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="931"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="289"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="486"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="568"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="687"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="753"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="780"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="965"/>
            <end position="991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1138" mass="116554" checksum="036F4E869E8A0CD0" modified="2014-01-22" version="1">MGRVWRATDVVLHREVAIKELVPPPGLTPDERREMRERSLREARAIARLNNINVVRVFDVLRTDADPWIVMEYVPSRSLQDILAADGPFAPVRAAEIGLGVLNALRAAHRAGVVHRDVKPGNVLISEDGRVVLTDFGLATVPGDPNVTRTGLVLGSPAYIAPERARDGTAGPGADLWSLGATLYAAVEGASPFARPSAIATLAALATENPPPARNAGPLKPVLNGLLRKDPAHRINAEEAERLLLRATGRRSKLSFPMSPTMRRPGVGRERPSAALSPPVVPGVSASAPVVPGPRPPVSPARPPVSPGRPPASAARPPVEPPAGGPPPRFTPGKAVVGRPPAETPTRVDGPKPPALDATKIENRSGTDTAPPTTPPASARPSRGAAPDATMRGSGSAPGSFTADEVAAARKRPTGRTAVPITPAVRPAPAASSGPAVADAPTEVKNPPAKRDSTAPATPAPAAGPATPAPATHAPAATAPATSAPAPAEPATPAPAPATPAPVPATPAPAPATTAPAPATTAPAASAPAAAASSPAAPAPVEAEQPEPPAKSTVTTPTDESAAPPVEAGTATDTQQPSTETPEAVDEASEARTPEAATSEAVTEAPEAAASEAAASEAATPESVTQAPEAVEETPEPTKPALAAASLATSKAARKKARAAGTTTKPDQPAEAVPAEAALAEAEPVTSKDAEPVENKDAEPATNKEATTPAEEEPTTAEPKAAEPVTAEPQAAEPVTAESQAAAPTTTEPKAEEPNPDTAESKAEEPKTAEAPTEPPTADSDPAPKTNSITPAAPETDGKPQAKPGTTPDRTRIIKPVTPADATKPRTTGGTPKRPQKTAAERKASTRVGAVPAARTEAASARAAAPTSGVPGFGQPSRPAWQPMNLREPSSGPRGITIFGTTLTRRQTVIAIAVLAAVLLALIVVIPQAFGGEEDKPDGRKAGVAPAASATKPTTAATTAAPAPAAPAPESPSPSPSSAAPSSPPPPAQGDVKIPKGWYRYSDDTGFSVPLPNGTSIERQGTEVYFTKNNRLLIIDQTDKPRPDPVADWEQQEDDRAGRNYRNYNRIKIQPVDYFVKAADWEFTYTTSSGNPQHAVKRGVITKAGKQAYGLSWYTSPGDWDAGKKDLQLIYQGFKPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAH3</accession>
    <name>U5WAH3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Lipid A export ATP-binding/permease protein msbA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_33440</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33440"/>
    <dbReference type="EnsemblBacteria" id="AGZ44946">
        <property type="protein sequence ID" value="AGZ44946"/>
        <property type="gene ID" value="AFR_33440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6768"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="317"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="351"/>
            <end position="358"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ44946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="561" mass="60892" checksum="BF01A50B6CA25156" modified="2014-01-22" version="1">MLVVLFQFVATLATLYLPTLNADIIDNGVVPGDTGYVMQVGLEMLGITLLQIASQVAAVYFGARTAMAVGRDIRAGIFSKVLSYSAREVDSFGAPSLITRTTNDVQQVQMLVLMTFLLMVSAPIMCVGGILLALHQDVPLSSLLLVIVPLLVAVVAAIIVRMRPLFRTMQVRIDKVNRVMREQIAGIRVIRAFVRDDRERVRFGGANEELTDVSLRVGKLMALMFPTVMLLVNISSVAVLWFGGHRIDDGSMQIGALTAFLSYLMQILMAIMMATFMFVMIPRAEVCAERIEEVLDTPSSVEIAEHPVTELSRHGHLELRDVDFHYPGAEAPVLSGINLTARPREITAVIGSTGSGKTTLLNLVPRLFDATGGTVLVDDVDVRALDPALLSKTVGLVPQKPYLFSGTVASNLRYGNPDATDEELWTALEVAQARDFVQRMDGGLDAPIAQGGTNVSGGQRQRLAIARVLVHRPEIYLFDDSFSALDYATDAALRAALTAHIADATVVIVAQRVSTIREADRIIVLDDGHVVGSGTHTELMDANPTYREIVLSQLTEQEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAL4</accession>
    <name>U5WAL4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Adenosine deaminase</fullName>
            <ecNumber evidence="1 3">3.5.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2 3">Adenosine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">add</name>
        <name evidence="5" type="ORF">AFR_39550</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2'-deoxyadenosine + H(+) + H2O = 2'-deoxyinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:28190"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17256"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine + H(+) + H2O = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. Adenosine deaminase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="1 3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562497.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39550"/>
    <dbReference type="EnsemblBacteria" id="AGZ46164">
        <property type="protein sequence ID" value="AGZ46164"/>
        <property type="gene ID" value="AFR_39550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8001"/>
    <dbReference type="eggNOG" id="COG1816">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039228_0_0_11"/>
    <dbReference type="OrthoDB" id="554648at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046936">
        <property type="term" value="F:2'-deoxyadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009168">
        <property type="term" value="P:purine ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00540">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028893">
        <property type="entry name" value="A_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="PTHR11409"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01430">
        <property type="entry name" value="aden_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="A_deaminase" evidence="4">
        <location>
            <begin position="13"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="3">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00031852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="355" mass="38783" checksum="5FF59F2FC44EFD9C" modified="2014-01-22" version="1">MVAITHADIVQAPKALLHDHLDGGLRPATIVELAAGIGHELPETDPERLGDWFVAAADSGSLERYLETFAHTVAVMQTEEGLHRVAKECALDLAADGVVYAEVRYAPEQHLERGLTLDAVVDAVHAGFREGCAEAAAQGNQIRIGTLLTAMRHAARSQEIAELAVRYRDTGVVGFDIAGAEAGFPPTRHLDAFEYLQRENFHFTIHAGEAFGLPSIWQAIQWCGADRLGHGVRIVDDITGTSLGRLSSYVRDKRIPLELCPSSNVQTGAAPSIAEHPIGLLHDLRFRVTVNTDNRLMSGTSMSREMALLAEAFGWGWSELQWFTINAMKSAFIPYDERLAIINDVIKPAYAKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBC2</accession>
    <name>U5WBC2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="4 11">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_40165</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="4 11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562619.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40165"/>
    <dbReference type="EnsemblBacteria" id="AGZ46287">
        <property type="protein sequence ID" value="AGZ46287"/>
        <property type="gene ID" value="AFR_40165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8129"/>
    <dbReference type="eggNOG" id="COG0113">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_11"/>
    <dbReference type="OrthoDB" id="677575at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="PTHR11458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ46287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="329" mass="35389" checksum="3C5FC9F2C29DB38E" modified="2014-01-22" version="1">MSFPDVRPRRLRRTAAMRRLVEETRVAPAELVLPLFLKEGLTEPRAISSLPGVMQHSRESLRKAAHEAVRAGVGGLMLFGVPDNANKDETGSGGINPDGILNVGLRDLISEVGDSTVVMGDLCLDEFTSHGHCGVLAPDGTVDNDATLELYGRMGVAQAEAGAHVVGPSGMMDGQIGVIRKALDQAGHQDVSVLAYSAKYAGAFYGPFREAVESTLQGDRRTYQQSPGNVREALREVELDVAEGADMVMVKPALPYLDVIAAVRERVDIPVAAYQVSGEYAMVEAAAANGWVDRQGVMLETLTSIKRAGAQIILTYWATEAAQLLRERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBD7</accession>
    <name>U5WBD7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glucokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41025"/>
    <dbReference type="EnsemblBacteria" id="AGZ46459">
        <property type="protein sequence ID" value="AGZ46459"/>
        <property type="gene ID" value="AFR_41025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8304"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_0_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="308" mass="30666" checksum="3DDA80322AB8BAF9" modified="2014-01-22" version="1">MSNDVVVALDVGGTGMKCALVLPDGTVHHAERHPTLASRGPAAVTENILEVAAGLADRARADGLTPVAAGVAVPGVVDEETGTAVWSSNVGFRDVPLRDLVTARLGLPAALGHDVRAGGTAEARLGAGRDQRHVLFIAIGTGIAAAHVVDGQAFAGAHGAAGEVGHVIVRPGGPPCGCGARGCLESIASASAVGRRYVELSGIEGVTAFDVATRAAAGEELANSVWTEAVEAFADGLLTAQALYDAGIVVLGGGLAEAGEALLAPLRVALDARITFHRMPQIVRAALGDTAGCLGAALLALDSLGAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBS5</accession>
    <name>U5WBS5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Winged helix family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_35775</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AfsR/DnrI/RedD regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561747.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35775"/>
    <dbReference type="EnsemblBacteria" id="AGZ45411">
        <property type="protein sequence ID" value="AGZ45411"/>
        <property type="gene ID" value="AFR_35775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7241"/>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004665_1_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15831">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005158">
        <property type="entry name" value="BTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03704">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01043">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="347"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="974" mass="102731" checksum="F0E09EFBFC7C5EC7" modified="2014-01-22" version="1">MLTCHVLGPTEIGAGRERVDLGGPMPRRLITALVAADGRPVSEDTLTLAVWGDDPPASPAVSLQAYVSRLRRALGQHRDALERVGDGYRLAGAGTDVADFEAGVARGRDLLGEQRPAEAVRAFDAALRLWRGEAFADLGASAEIETDRVRLAELRLVAVEERLAALLAVGDAPGAVAGLTPAVREEPYRERRWELLILGLYRSARQAEALSALRRVRSLLADDLGVDPGPALQSLERRLLSQDPMLLLPPPPSPPSPLPPTSSEVTRPLSTFVGRTAELGTLTATMRTARLMTLVGPGGAGKTRLAVEYAATSTGSDGPWLARLADVTDPALLLSAVATALGIRETPATDDRTGATDGLASTDLAGSGSPVTSASEWRPGPGVGGSGGLYSDGRTTRAFEGSATGKALVSALTGREALLILDNCEHLVGAAADLVVGLLARCPRLRIVATSREPLGVDGERLLPVTPLPSGAAVALLVDRIGAIRPGWQPDPEDLAELARLAEALDGIPLALELAAARARVLGLGELVALLGERFPELGRVPRGALTPHATLEATVAWSVDLLSVADRALLLRLWPFEGGFTLEAAAAVGSDLEALSALVTRSVVAADTTVAPTRYRLLEIIRAFCRERDPDPAASRAAHAAWVRALVDRSSKELRGERSARAIRVLNRELPNLRAALAHDLVADPAAALRTAGRLEWFWFRGGHVSEGLRLLTAALAGAPAADDVTRAQAWASCGILHYIAGNLDRAAECLRQAKDLLAEPPDRDGWRLLGQVLYYEGLLWSLWGDFDRSRLRATESIAVSRRIGEEWIEPAGAMTLGTALVGLGQVEEGRTVLADAAAHAGRLGQNWIAGMSELLLARSWLQVADPSPAAALDALRGAVDRFREEDDIGDVLACLHSGAYALVLSGRLAEGATLLASVRRHGRRRGMSLEASDPATSAALAGAGVPEAAGTLDEAAMIALLQSGQDEEHARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBW3</accession>
    <name>U5WBW3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_42145</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563014.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42145"/>
    <dbReference type="EnsemblBacteria" id="AGZ46683">
        <property type="protein sequence ID" value="AGZ46683"/>
        <property type="gene ID" value="AFR_42145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8528"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066229_0_0_11"/>
    <dbReference type="OrthoDB" id="1751860at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="36605" checksum="4C89C62994E8DE71" modified="2014-01-22" version="1">MIWMTWRQFRTSALTTGAVVLVFLAGLALSWAEVRELATQTGFTGCQGDGCRDAGEAFLRALQPSWMSEFHIATIGAVYLLPALVGIFWGAPLVARELESGTYRMVFSQSVSRGRWLLAKLAMGGGAAVLGAGLLSLMLTVWAQPIDGASADRMNPLVFAARGIVPIGYAALAFVVGATAGLLLRRSVAAMAVTLLVIVSIQVAMPFVVRPWLAQPVTTTAPLQVEGDFGISMNPDTREMTIEVEPDLRGDWIVSSTTLTSTGAEFKGPADLTQCGPDAPRDRETCPRWLEGQGLELEVVSVPASKFWTLQWREFGALFVLTAALALFSLWWIRRRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCB5</accession>
    <name>U5WCB5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="7">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_42725</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563130.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42725"/>
    <dbReference type="EnsemblBacteria" id="AGZ46799">
        <property type="protein sequence ID" value="AGZ46799"/>
        <property type="gene ID" value="AFR_42725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8647"/>
    <dbReference type="eggNOG" id="COG0303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_1_2_11"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6 7" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="8">
        <location>
            <begin position="176"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="408" mass="41991" checksum="1C0DABA828075BB8" modified="2014-01-22" version="1">MSAQHLPVDWDKARTLAYEAGRAAAGPAETVPLAGADGRTLAEPLVTLTDLPAFPTSSIDGWAVRGPAPWRPTGRVLAGGTAEPLTGDGTCVEIATGAMVPAGTEALIRVEESTRDSDGRVNGTPRPLPEWRLPGDEAHQGEELLPAGTPVDPAVLGMAATCGYDTIVVRPLPRAALLVFGDELLTSGPPGAGRVRDSLSPLVPAWLRRYGATVDPSAVRGPVQDTLEAHVAAIRSALEVADVVCTTGGTMNGPVDHLHPALAELGAEYVMNTVAVRPGFPMLLARVPGPAGRPRFLAGLPGNPQSAVIALISLVGPLLAGLHGRDLPVLPRVELTAPVPGRGGFTHLALAALDQDGRKATPVGHVGSAMLRGLAGSHGFVVVRPDTQAAAGELVPFLPLPLYAGERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCE9</accession>
    <name>U5WCE9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562011.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37105"/>
    <dbReference type="EnsemblBacteria" id="AGZ45675">
        <property type="protein sequence ID" value="AGZ45675"/>
        <property type="gene ID" value="AFR_37105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7507"/>
    <dbReference type="eggNOG" id="ENOG5032UIF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123725_0_0_11"/>
    <dbReference type="OrthoDB" id="1873256at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="164" mass="17734" checksum="8E9F1AE36D92C89B" modified="2014-01-22" version="1">MDHKVWMYLVYLIVSTGLTVWVATTLSRNGLVFLEDVFADKRLARAVNQLLVMGFYLLNLGYVAVAMRSSARIPDAARALETLSYKIGFVLLVLGVLHVLNVFFLGRYRRGRLRQQQVMPPLPPAGRLPMYPGPGGPSAGAPGAPPAGWPGQGMPGPVPPPTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCF9</accession>
    <name>U5WCF9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_37155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562021.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37155"/>
    <dbReference type="EnsemblBacteria" id="AGZ45685">
        <property type="protein sequence ID" value="AGZ45685"/>
        <property type="gene ID" value="AFR_37155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7517"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_2_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="33466" checksum="56B73E63EF9449FE" modified="2014-01-22" version="1">MALEFRFDDIPDQTGRTVVITGASSGLGLIAARELAARGATVIMAVRDTDKGHRVRAGLRGDLEVRHLDVADLDSVRAFSAALHEQGRTIDVLLNNAGTGEQTRRLTPQGHERVFATNHLGHYALTGLLLDLFRPDRDPRIVTVASNFYSRLRTGLDFGNLQGERSWSAGSAYVRSKLANVLFGAELERRLRRTDSPVRSFLAHPGMATTPMHDSNAGLAQKAFVAIATPLLARSAERGTEPILFAATSPAAEPLTFLGPSIRKWDSKVHFAPLVPPGDDLAAGSRLWSVSADLTGVRYLADDTSRLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCP3</accession>
    <name>U5WCP3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_42440</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563073.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42440"/>
    <dbReference type="EnsemblBacteria" id="AGZ46742">
        <property type="protein sequence ID" value="AGZ46742"/>
        <property type="gene ID" value="AFR_42440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8589"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_11"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="10"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="139"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="139"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="235" mass="25516" checksum="571FC0B2D0B828A1" modified="2014-01-22" version="1">MGVGDVTARKVLVVEDERTIAEAVAARLRAEGFEVEIAGDGPSAVDAARRTLPDAIVLDVMLPGFDGLEVCRRIQAERPVPVLMLTARDDETDLLVGLAVGADDYLTKPFSMRELSARVHALLRRAVRNAAPDATAVASPTMHLGDLEINLAERRVLRAGVEAKLTPTEFDLLVHLAGRPRTVLPRERLLADVWGWADASGTRTVDSHIKGLRRKLGADLIRTVHGVGYALEVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCQ2</accession>
    <name>U5WCQ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42490"/>
    <dbReference type="EnsemblBacteria" id="AGZ46752">
        <property type="protein sequence ID" value="AGZ46752"/>
        <property type="gene ID" value="AFR_42490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8599"/>
    <dbReference type="eggNOG" id="COG3162">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123372_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007436">
        <property type="entry name" value="DUF485"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04341">
        <property type="entry name" value="DUF485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="15579" checksum="0EDCAFE355A7A63D" modified="2014-01-22" version="1">MTTETTPTEKYLEVQQSEEFTRLRRTLRNFVFPMTVAFFLWYALYVLLSAYARGFMSVKLVGNINVALLFGLLQFVTTFLIAWFYSRFAARQVDPIADKIREELTAGAAARTSATTTTTTATADATPTEDKPEPEEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDW0</accession>
    <name>U5WDW0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_39235</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562435.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39235"/>
    <dbReference type="MEROPS" id="S54.029"/>
    <dbReference type="EnsemblBacteria" id="AGZ46101">
        <property type="protein sequence ID" value="AGZ46101"/>
        <property type="gene ID" value="AFR_39235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7939"/>
    <dbReference type="eggNOG" id="COG0705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067823_2_0_11"/>
    <dbReference type="OrthoDB" id="2053476at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="SSF144091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Rhomboid" evidence="6">
        <location>
            <begin position="69"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="23692" checksum="38EBB0CD048FCCBF" modified="2014-01-22" version="1">MGYPGEREDQAARFGTEAFFASLGRAFVAMCAVIPVLFLIEAIDVGLGAGRLDVAGGIIPHRIDGLDGVIFSPFLHAGWDHLYGNSIPLILVGTFALAGGTRRFLWSTLVIVLVSGFGVWLIGDPSSVVVGASGVIFGYLGLLFARGFVERSWWNLGVAAFIGLLYWYQLYNVLPTDQPISWQGHLLGLLGGVISAIAFRRRRPRPAPSLYDPGATLTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDY1</accession>
    <name>U5WDY1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39880"/>
    <dbReference type="EnsemblBacteria" id="AGZ46230">
        <property type="protein sequence ID" value="AGZ46230"/>
        <property type="gene ID" value="AFR_39880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8069"/>
    <dbReference type="HOGENOM" id="CLU_1399875_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="208" mass="21506" checksum="EBDA0567D4F6019C" modified="2014-01-22" version="1">MTEAAFFVSRAIVRCLQPSGFRLVAVSIRGWQLAGAGLVVLALVAAGAFALRPRGSTEAVVSTCLRLEASDEQQAAAEEALCDRRALDEARRAPVPAGAVQDSSATDIYKALNKAICHPPGGSICRRGSRPQPATDADVGVVRQIIADLRFGDAVVRLSGPGDPAPDGAVVYGVRLGGSYCAVSYVQMGVGAYPGDVRGLLPNGQCLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEF9</accession>
    <name>U5WEF9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562658.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40360"/>
    <dbReference type="EnsemblBacteria" id="AGZ46326">
        <property type="protein sequence ID" value="AGZ46326"/>
        <property type="gene ID" value="AFR_40360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8168"/>
    <dbReference type="eggNOG" id="COG1387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072555_0_0_11"/>
    <dbReference type="OrthoDB" id="1615112at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="25944" checksum="0163A86154F70BB7" modified="2014-01-22" version="1">MGAERESVLDGTALDLRADAHVHTGFAAGRDSVGVVVSAADLAGMTAVTFADQVGLETTWLPAYADTIRRARRRTELVLRVGAEVEVVRQDGWLAWPADLGHLEVVSVAVSQLPQADGPMTAREVRSLLAAGRLTPEQVAESVVTATIKGIERASRYAPVQLARPLSLLTQVGLDDTAMTPELLSELASACRHTGAAVEISEAWRSPSARVVALLREADVALVAASDARYAGEVGRWQYLRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFS2</accession>
    <name>U5WFS2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP_transf_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563112.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42635"/>
    <dbReference type="EnsemblBacteria" id="AGZ46781">
        <property type="protein sequence ID" value="AGZ46781"/>
        <property type="gene ID" value="AFR_42635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8628"/>
    <dbReference type="eggNOG" id="COG0746">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055597_1_2_11"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="2"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="193" mass="20506" checksum="D3F02701140BF637" modified="2014-01-22" version="1">MVLAGGAARRMGGADKPTIPVAGQSMLTRVLAAVHDADPQVVVGRVPPDLPVPVASTSEQPPGGGPVAATAAGLELVPDNVSFTALLAADLPLLTGEAIDVLRLTVESAPMQGAIYRDSEGRRQMLCGVWRTSALRQALGKVAEERGSLHGASMQTLLEHLRFIEVSWRRPGPPPWFDCDTDDDLRTAEEWAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VND0</accession>
    <name>U5VND0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00090"/>
    <dbReference type="EnsemblBacteria" id="AGZ38307">
        <property type="protein sequence ID" value="AGZ38307"/>
        <property type="gene ID" value="AFR_00090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.19"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096760_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="19162" checksum="F3B085EE5354937F" modified="2014-01-22" version="1">MNSIRPTRHSDLVVLQQIELAAGALFRDVGMPDIADNPLPTIEALAAFQEADRSWVTVDDDDQPIAFVLVMIVDGLAHVEQVSVSPAYARQGLGRRLIDHVDAWAVEQGMAALTLTTFRGVPWNGPYYQRIGFVTLAAEERGPELTQLMVQEAAHGLDPAERIAMRRPTRLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNG1</accession>
    <name>U5VNG1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF397 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357281.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00240"/>
    <dbReference type="EnsemblBacteria" id="AGZ38337">
        <property type="protein sequence ID" value="AGZ38337"/>
        <property type="gene ID" value="AFR_00240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.49"/>
    <dbReference type="eggNOG" id="ENOG50333C3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131550_1_1_11"/>
    <dbReference type="OrthoDB" id="1996962at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007278">
        <property type="entry name" value="DUF397"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04149">
        <property type="entry name" value="DUF397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF397" evidence="1">
        <location>
            <begin position="15"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="74" mass="8082" checksum="C970250A9B780613" modified="2014-01-22" version="1">MEVETKGFRVDLNRAQWFKSTRSGPNCDNCVEVAFVDEAIAVRDSKNPAGPALIFTAAEWDAFVGGAKDGEFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNL1</accession>
    <name>U5VNL1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_00490</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357331.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00490"/>
    <dbReference type="EnsemblBacteria" id="AGZ38387">
        <property type="protein sequence ID" value="AGZ38387"/>
        <property type="gene ID" value="AFR_00490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.100"/>
    <dbReference type="eggNOG" id="COG1071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_1_0_11"/>
    <dbReference type="OrthoDB" id="1248201at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016624">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017596">
        <property type="entry name" value="PdhA/BkdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF41">
        <property type="entry name" value="PTHR11516:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03181">
        <property type="entry name" value="PDH_E1_alph_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="E1_dh" evidence="4">
        <location>
            <begin position="68"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="391" mass="42611" checksum="434BB7497FC3279C" modified="2014-01-22" version="1">MANGESEVGAAPLPAEDRSPGSDAPAGGFVQLLTPGGERIDSVTTADGVTYSVDFTDDEYRELYRDLVTVRRLDAEATALQRQGELGIWASLLGQEAAQVGSGRALRPQDMAFPTYREHGVLYTRGIDPIMPFGLFRGVDQGGWDANKYKFNGYTIVIGSQTLHATGYAMGVTMDGKTGTPDGEAVIAYFGDGATSQGEVNEAFVWAGVFNAPMVFFCQNNQYAISEPLERQTRIPLYRRSAGFGFPGVRIDGNDVLASYAVTRAALDNARNGQGPTLIEAYTYRMGAHTSSDDPTRYRIASEVESWKAKDPISRLKAFLTKQQLADDSFFGEVDDTARTLALDLRERVLAMPDPQPVSMFDHVYPTGSPEVDAQREQFAAYQASFEGSDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP66</accession>
    <name>U5VP66_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vitamin B12-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01515"/>
    <dbReference type="EnsemblBacteria" id="AGZ38592">
        <property type="protein sequence ID" value="AGZ38592"/>
        <property type="gene ID" value="AFR_01515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.304"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_8_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="14"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="28475" checksum="E6320A083CD8A643" modified="2014-01-22" version="1">MTAGGVTLAQRPERIVSLGPSATEMLFAIGAGPQVVAVDDQSTYPADAPKTDLSGFKPNAEAIAAKNPDLVVATDDLNKIVDQLGKLKIPVLLVPAAKVLDDTYREIDELGRLTGHQTEATALTDRMRTQIDKIVKGVPARPSKLTYYYELDPALYTATSKTFIGSVLGLFSLENVADPSDADGSKGGYPQLSPEALIKANPDLIFLADTKCCQQSPATVKARKGWSAVTAVQKDQIVSLDDDIASRWGPRVVDLVQSVADAVSKVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPT2</accession>
    <name>U5VPT2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome P450</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03395</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38968">
        <property type="protein sequence ID" value="AGZ38968"/>
        <property type="gene ID" value="AFR_03395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.683"/>
    <dbReference type="eggNOG" id="COG2124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033716_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002397">
        <property type="entry name" value="Cyt_P450_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00359">
        <property type="entry name" value="BP450"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="SSF48264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="399" mass="43694" checksum="7AB41C77679C837C" modified="2014-01-22" version="1">MESPEVTTLLDELTRLPGRDDPYPRYRRLREISPVVRAEDGALVVTRHADCVAVTRHPKLGHMPSHMMEFVRPDWDRHPALQQLFTSILAINPPDHTRLRRLVSSSFTARRVQALQPRIAQMVEELLEPMSGEVDFVQAFGFPLPVNVIGELLGIPEPDRAQFQTLVRDWTQVLEVISPEVLATADPAAATIRSYLAGLAAERRRKPGDDLISALVAAEEDGEKLTEDELLTMAALLFAAGFETTTNLLGNSVVSLLRNPDQLELLRMQPGLAASAVEELLRFDTPVQLLSRVAWDDIEIAGVPVSGGERIVAYLGAGNRDPERFTDPDRLDLARPDNAPLSFGGGIHYCLGAPLARLEAQIAIPALLARFPGLALAGEPGRRDSLAIRGYTSVPVTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ83</accession>
    <name>U5VQ83_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_03945</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03945"/>
    <dbReference type="EnsemblBacteria" id="AGZ39078">
        <property type="protein sequence ID" value="AGZ39078"/>
        <property type="gene ID" value="AFR_03945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.797"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="19"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="144"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="144"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="27247" checksum="5AD6FCE63A866C4B" modified="2014-01-22" version="1">MANADTSTELRRPDGGPVRVLVVDDEPTLAELLSMALRYEGWDVRSAGDGLTAVRTARDFRPDAVVLDMMLPDIDGLEVLRRLRGESPEVPVLFLTAKDSVEDRITGLTAGGDDYVTKPFSLEEVVARLRGLMRRMGHSPMRTESQLIVGDLSLDEDSHEVRRSGDLVTLTATEFELLRYLMRNPRRVLSKSQILDRVWNYDFGGQANVVELYISYLRKKIDAGRNPMIHTMRGAGYVLKPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQS9</accession>
    <name>U5VQS9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358849.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05170"/>
    <dbReference type="EnsemblBacteria" id="AGZ39323">
        <property type="protein sequence ID" value="AGZ39323"/>
        <property type="gene ID" value="AFR_05170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1046"/>
    <dbReference type="HOGENOM" id="CLU_1352217_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="202" mass="21339" checksum="F5CE537B9E4384BC" modified="2014-01-22" version="1">MQPSTSRYQFSYGFTTSRSTRLLFLTFAGLLGVPAAGCVGAIVGGGIATLFDEPGPNGRVLIVIPIILVLLLGGGMLAWLVVSIRTVAKMGASLEGTRLTVRERRSATVDLARARSVSLQPSRIRWKTPPHAGVATSDQIPEVVVIGDEGEVRMRLSNAERVPMSPQELHILAGVLRTVQAPGAAQVAQHLQVLAGSAPNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRG0</accession>
    <name>U5VRG0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TPM_phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_06245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06245"/>
    <dbReference type="EnsemblBacteria" id="AGZ39538">
        <property type="protein sequence ID" value="AGZ39538"/>
        <property type="gene ID" value="AFR_06245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1267"/>
    <dbReference type="eggNOG" id="COG1512">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013689_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007621">
        <property type="entry name" value="TPM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04536">
        <property type="entry name" value="TPM_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665992" description="TPM_phosphatase domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="TPM_phosphatase" evidence="7">
        <location>
            <begin position="34"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="618"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="648" mass="66655" checksum="966A62EE134EAA12" modified="2014-01-22" version="1">MKFLGRTAVALVALLLLAPAPAQADAPSRLAVQVTDAAGALGSRAAAVDSALAALQRDTDVQLWVIFVDSFDGTPAEQWTEATARLSDLGDSDALLAVATQDRSYYVSFPADPRFTDAELADVANRDLEPALSRGDWPGAVIAAASGYSDAANSSNSSLIWVVVIFVVILAGALIWVVLRRRRARPAPAAPTGPPTAELTASANALLIELDDDLRASESELSLATAQYGAEATAPFKAALEASRQDVAEAFRLRMTLEEVPAPDEETRRRTLTEIIAKCRAADDRLDAESEAFDRLRDLEGRAAEVATELETRRLTLEATLPAASKTLQDLTHRYAGPTVTGISGNLDQARERLQFTAEAIGRARTALPANRAEAALAVRAAEQAADQAAQLITAIDRAAADFTTARTAADALLTELESEIAAGRAALTGGSVVPPGLGSAVTGAEQAVVEVRTQLAAPKTDPVTAVATLQAADAALDHALAEAQDAAERTARARSLLAQALPVARAEVAATTDFITTRRGAITTTARASLSEAQRHLALAESLAATDPAAALTEAQQAQQLAAAAGHSAHTDVQGWGGYTTQPTGGFDAGAFAGAVLGGILSGGGRSYGGGRSGGWGGGGFGGSATRTRRTGGSSGGSARRGGGGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRI3</accession>
    <name>U5VRI3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F420-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359005.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05925"/>
    <dbReference type="EnsemblBacteria" id="AGZ39474">
        <property type="protein sequence ID" value="AGZ39474"/>
        <property type="gene ID" value="AFR_05925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1204"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_6_3_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="31819" checksum="370C73C65D9561BB" modified="2014-01-22" version="1">MADFALQAQPTDAADWLDLARRAEAAGFDTLLAADHPGACASPFVALAAAAAVTTTIRLGSYVSNAAVREPILLATDVATLDVVSGGRAVLGLGAGHTPAEWDAIGRTRPDVRGRVDRCIAVARATMALLAGEKVTVDTPELTMYAAELTEPRPVQERVPLTIGTANSRLLRWAGEHADTVGLTGFGRTLADGHQHEARWQLAEIDAQVELARAGGAATLEALVQRVEITDDAGSALAEDAGRLGMSETDLQQVPFQLVGTEDEIVAAVRAHRERWGITRYAVRRAAVDALTPLLPRLAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRS4</accession>
    <name>U5VRS4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357379.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38435">
        <property type="protein sequence ID" value="AGZ38435"/>
        <property type="gene ID" value="AFR_00730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.147"/>
    <dbReference type="eggNOG" id="ENOG50331V5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_400982_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="SSF49464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="686" mass="67886" checksum="13939DF641C9A192" modified="2014-01-22" version="1">MTSLSGNVPSGGQTVLKYKVFNKNLNVPGAETGVAIVIQADGMECDKCDFSDDVAPQQTKEFSATLKAGNVAPGETQQVQVSISATIGNSPAGTAGRRVIIRGEDKPETVRQVSGKVKDQDGKAVSGALVGLQDSQGHQFSTNSNGDGGYSFTSSDDKPISVGVMAVGANKEGYKVTSVNVQGKAGKSVNVPLTLTSTTPTSPSASPSPSASPTTEPTDEATDDETAATDESAAAADTTPTSGDDEGSGSLLYIILGGLLVAAGIGAIVLVLMRRKAGDDPDGTDGPDSPLGVPPAGGGGRYAGADETRLAGAPVGGLASDATMIAPRSGAPSMADAPTMIHQAAPALDEFPDPYGAPIPQGGGYNAPGGWGTAGAAAGAAGAYGTATQFGGAPVPPQNGGYADQDDNAYGAYGAPQGGYNDADGYDQAAPYGAPQGGYEAPQQRYDEPTGMYRPEPNGGYPPEAAYPDQGGYGAEPAYGQPGYGQPGYGQGADDYAAPAAQPPVQGGGYQGGAYGAPAEPADQGGGYGAWDGQDDGGAYAPPAGGGTYGGGAAGTYGGAPAQGQPYGGDNYGGAPAGGDNYGAPAAGGGYGGAPAGGYGADQGGYDPRATYGRPEGYDQQGGGRGQQPPPPQGGGYGADQGGYGADQGGYYGADQQQGRHGGQPRQQPPEQARPGQRRPLDYLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRW4</accession>
    <name>U5VRW4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative capsular polysaccharide synthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359100.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06400"/>
    <dbReference type="EnsemblBacteria" id="AGZ39569">
        <property type="protein sequence ID" value="AGZ39569"/>
        <property type="gene ID" value="AFR_06400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1298"/>
    <dbReference type="eggNOG" id="COG3071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_410309_0_0_11"/>
    <dbReference type="OrthoDB" id="604239at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008441">
        <property type="entry name" value="AfumC-like_glycosyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05704">
        <property type="entry name" value="Caps_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666006" evidence="2">
        <location>
            <begin position="28"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="321"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="669" mass="74215" checksum="2F302D01140A9245" modified="2014-01-22" version="1">MRPNTSGLRRRLTQAATGNLRLPASRAARLVAGPAPVVNLAPNPSFRSAQADGTFDQPLDAGGVYVHFHSAARSAPVPGLGVTGALVTGSGTNNDSHIAPGGRDESQDFRLGLRAGGTYTASVSVFLTEPLTGAINPYALRIIPGCVRGGTANFHLTASPPARNEYGDHRISVTFTLPENATAAWVRLLAGMSQGHGQVYWHSFSLTEGEQHVTYFDGDTEDDNFFTYEWLGEPGASASRRTLRAYQEILTRTDRQGVADEAARLSRAGEAAEADLLVAALAGELDPISRLTTARRLRDRDEIPQARQTLRKMIKAGDEHGDAAFELGRLHERKHDWAGAEQLYRDAVEKKPGDSERFYRLAYTLDKLKRRDESKQTSRQGLAVDGELPFDGPAVLDLDVKCFGARREIGIFLSEHLDQIRTQARQRLDRPARTALEMPIFVYWAQGFESAPALVKRCVAELRANNPQAQVHELSDTNLGYYVDMPVDLVTALGDNKTNFSDLVRLALLEKFGGIWVDATVYAPKALSEPVGAALGDGDFFAFNYHGPYISNWFLAARPGSYVAHLWRAAMYLWWEKRGELIEYFLAHHIFEMLYHLDADFAAEWDKGNRVSTRPAHALQVAMLQPYEPESFERLLTGSFAHKLRYKYQPQQVMSDSNLAHLVRGDHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSE9</accession>
    <name>U5VSE9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_01730</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01730"/>
    <dbReference type="EnsemblBacteria" id="AGZ38635">
        <property type="protein sequence ID" value="AGZ38635"/>
        <property type="gene ID" value="AFR_01730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.349"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058822_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="377" mass="37196" checksum="716E0F55D2F3BD7C" modified="2014-01-22" version="1">MTGALTTTIACSVPVFLVGGLAVQIGEELRFSPAGLGLAVSAYFGASALASVPAGALVERFGSTTIARAGIALASVSLLTIAVAARSLWTLIAILALGAAANALGQLASNTSLSRHVPVRRQGLSFGVKQAAIPVSTLLAGAAVPVVALTVGWRWAFVLAAVGAAGALWLVPSEKDETKRRQTGGGERATAALIVIGVGATLAAGSANALGTFLVDSAAARGMAPGTAGLALTLGGAVCVIARVFGGWQADRFPSRQIAVIAALLAVGAVGLALMAVAGTVTLVAGVVLGFGFGWAWPGLMNFAVVRLHPQAPAAATSITQTGVYAGGCLGPLGLGALAAAAGYPVMWTVAAIAMVWAAAAMVVGSRMLARHAAAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSK6</accession>
    <name>U5VSK6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359380.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07800"/>
    <dbReference type="EnsemblBacteria" id="AGZ39849">
        <property type="protein sequence ID" value="AGZ39849"/>
        <property type="gene ID" value="AFR_07800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1592"/>
    <dbReference type="eggNOG" id="COG2258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092690_0_0_11"/>
    <dbReference type="OrthoDB" id="1877633at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="18"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="262" mass="26896" checksum="60BE7B77CC37C8E4" modified="2014-01-22" version="1">MQGAVAAVSCNDVYSFTKPTRGEITLVAGLGVVGDVHAGVTVRHRSRVATDPQQPNLRQVHLIHAELHDEVRPEGHEVPAGGLGENVTTTGIDLLGLPVGTILHFDPPAGSVTTSVPAAPAAPRFVADGLFEVEGPPGGAAVVLEAAAKATLEPHIAPAVAILAARIEAADQSGATEEQPGDRADGESQGPAVIIAGLRNPCVQIDRYQPGLLKQVLGRSPDGRIVRKAGVMAVVLRGGTIRPGDAITVDLPPGPHVALDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSM6</accession>
    <name>U5VSM6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359524.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08520"/>
    <dbReference type="EnsemblBacteria" id="AGZ39993">
        <property type="protein sequence ID" value="AGZ39993"/>
        <property type="gene ID" value="AFR_08520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1733"/>
    <dbReference type="eggNOG" id="COG1300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069787_0_0_11"/>
    <dbReference type="OrthoDB" id="1745181at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="36130" checksum="C493DFE289C1099E" modified="2014-01-22" version="1">MDLDAYVAERRGEWNRLEVLARRRRLSPDEADELVLLYQRAATHLSVVRSHSPDPILLASLSQLVIAGRSAVTGGRRFSWRPVGRFFTSSFPAELYRTRHWWLGVMVINVLLGWALMWYFSTNPDIISSFGTGQELQQYVEKDFVGYYSEFQAQNFAASVWSHNALIAAQCLASGVLILPVLYILGQNLFAIGMIGGVMIYAGHGDTFFSFILPHGFLELTSIFVAAGVGLRIGWAWIAPGPHRTRGQALTAHARGGMLVALGLVAMLAVSGVLEAYVTPSALPTSIRIGIGAAVWLLFLAYALVLGAAAHHRGETGDLAPEHTETPVPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSS4</accession>
    <name>U5VSS4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_07865</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359393.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07865"/>
    <dbReference type="EnsemblBacteria" id="AGZ39862">
        <property type="protein sequence ID" value="AGZ39862"/>
        <property type="gene ID" value="AFR_07865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1606"/>
    <dbReference type="eggNOG" id="COG1120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_11"/>
    <dbReference type="OrthoDB" id="1048863at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="28525" checksum="D2D592265C3A838E" modified="2014-01-22" version="1">MSLAAKGITIGYDKKTISERLDLDVPESRFTAIIGPNACGKSTLLRALSRLLKPTAGEVLLDGRDIHSRPAKEVARRLGLLPQSSIAPDGITVAELVARGRFPHQKLIRQWSPDDEAAVVAALAATGVTELSGELVDTLSGGQRQRVWVAMVLAQQTPIVLLDEPTTFLDIAHQIELLELCVRLNREQGTTMVAVLHDLNQAARYADHLVVMKAGAIVAQGDPRKVMTAALVEDVFGLGCRIIDDPETHTPLVIPRSSVTQRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT85</accession>
    <name>U5VT85_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug resistance protein mdtL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02910</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357814.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02910"/>
    <dbReference type="EnsemblBacteria" id="AGZ38871">
        <property type="protein sequence ID" value="AGZ38871"/>
        <property type="gene ID" value="AFR_02910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.585"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038683_0_1_11"/>
    <dbReference type="OrthoDB" id="626204at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="26"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="409" mass="40922" checksum="62FF1A20E8E874F9" modified="2014-01-22" version="1">MTSTTITPAASTDEQAVRRKRLSRPVAFAAIAAVFVTFTAASSAPSPLYVVYQELWGFSASTLTAVFAVYVVGLIAALLVLGALSDHIGRRPVLGAAIALEIVALVLFLAAGDVPVLLVARFLQGIATGAALTTLGAALVDLNPPHAPGRAGLLNSVVPPAGLAIGSLGTGALVQFAPGPTHLVYALLLVGMALAAVVVLLMPETSSRRPGGRSSLIPKLGVPARLRGAVLALVPIIVASWALGGLYLALGPSVAVGVFGLSNHLIGGLVVTLLCGTGAVTAFALRTWPTPRVLTIAGALLTVGTAVTLAGVLTESVVLGAVGTVLAGIGFGASALASFGTIALLAAPHERGELFAVALTIAYVAFSLPAVIAGFAATSVGLHTTAVVYSTVVVVLGVAALAAQRLRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTS4</accession>
    <name>U5VTS4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Anaerobic regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_09590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359859.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09590"/>
    <dbReference type="EnsemblBacteria" id="AGZ40207">
        <property type="protein sequence ID" value="AGZ40207"/>
        <property type="gene ID" value="AFR_09590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1955"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_3_4_11"/>
    <dbReference type="OrthoDB" id="1596937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="17"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH crp-type" evidence="3">
        <location>
            <begin position="151"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="24963" checksum="344AB59870063D53" modified="2014-01-22" version="1">MDHRLAEQGDALTGVEMFAGLEPDVRQRVIAAAVPRTYRKGQILFVEHDPGDSLIILRRGAIAVFRTAPTGERAVLTVVRPPDVLGEVSLLDALTRSASAEAIEDSQALALSRVAFIDLVHSNPRILDAVMRSVGALIRRLTEQNADHVFLDLPGRVAKTLVRLAGESQAPMITIELNQSQLAEMAGGSRQSVNQAIGSFASRGWLRTEGRRIVVTDVSALRRRAGMADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU49</accession>
    <name>U5VU49_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360116.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10240"/>
    <dbReference type="EnsemblBacteria" id="AGZ40337">
        <property type="protein sequence ID" value="AGZ40337"/>
        <property type="gene ID" value="AFR_10240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2086"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1287934_0_0_11"/>
    <dbReference type="OrthoDB" id="1914615at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017517">
        <property type="entry name" value="CHP03083_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017519">
        <property type="entry name" value="CHP03085"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03083">
        <property type="entry name" value="TIGR03083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03085">
        <property type="entry name" value="TIGR03085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="22870" checksum="1034E7AD42EFF179" modified="2014-01-22" version="1">MTDYARSERRQLADLLLEVGPDVPTVCVGWTTRDLAAHLVVRERRPDAMIGALIPPLAGHGEHVRLAKAAQPYTEIIHEIRTPPWWSPVSNPLVDSLSNTVEFFIHHEDVRRAAPGFTRRELDPGEQKAIWNAAKLTARLALRKVGFAVLVEAPGHGSVRIGDGEPRATITGDPGELALFSSGRQRVAEVDVTGDPDAVEKLTNARLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUA8</accession>
    <name>U5VUA8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05105"/>
    <dbReference type="EnsemblBacteria" id="AGZ39310">
        <property type="protein sequence ID" value="AGZ39310"/>
        <property type="gene ID" value="AFR_05105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1033"/>
    <dbReference type="eggNOG" id="ENOG5031U83">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188863_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="89" mass="9346" checksum="3A727C82345AD9F1" modified="2014-01-22" version="1">MTPPPYYGQQPPAAPGNDKVTLWGVLGIVFAFCCWPLGIVFGVLSLLEARKVGKQPTLAYVTFGVIVLALIVNIILAATGALGDIMSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUP6</accession>
    <name>U5VUP6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA acetyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_05410</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358902.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05410"/>
    <dbReference type="EnsemblBacteria" id="AGZ39371">
        <property type="protein sequence ID" value="AGZ39371"/>
        <property type="gene ID" value="AFR_05410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1096"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_11"/>
    <dbReference type="OrthoDB" id="330994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="290"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="413" mass="43593" checksum="C959B27286B924F8" modified="2014-01-22" version="1">MPEAVIVATARSPIGRAFKGSLKDLRPDDLAATIIDAALSKVPQLDRTLIEDLYLGCGLPGGEQGFNMARVVATLLGLDGLPGATVTRYCSSSLQTTRMAFHAIKAGEGDVFLSAGVETVSRFARGNSDSLPPDAQALVGGGWENPKFAEARARTTENAKTGGVWHDPREDGQLPDIYLGMGYTAENLAQAYDVSREDMDEFGVRSQNLAEKAIADGFWEREITPVTTPDGRVISTDDGPRPGVTMEAVSGLKPVFRPDGRITAGNCCPLNDGAAAVVIMSDTKARELGLTPLARIISTGVSALSPEIMGLGPVEASKQALKRAGMTIDDVDLVEINEAFAAQVIPSYRELGIPLEKLNVNGGAIAVGHPFGMTGARITGTLLNSLEWHDKTIGLETMCVGGGQGMAMVLERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV42</accession>
    <name>U5VV42_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360726.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11890"/>
    <dbReference type="EnsemblBacteria" id="AGZ40667">
        <property type="protein sequence ID" value="AGZ40667"/>
        <property type="gene ID" value="AFR_11890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2421"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108054_0_1_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase_6" evidence="1">
        <location>
            <begin position="8"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="127" mass="14153" checksum="AED85BEA733E98DB" modified="2014-01-22" version="1">MVASIHNITVDTTGDPYELAQFWSSVVGRPLHVEDQPGDPEASLMAEGQPTMLFIRVPDGKAVKNRLHLDLQPQDRTRDEEVDRLIGIGATLFEDHRRPDGTGWVTLTDPEGNEFCVERSLAERTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV47</accession>
    <name>U5VV47_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11915"/>
    <dbReference type="EnsemblBacteria" id="AGZ40672">
        <property type="protein sequence ID" value="AGZ40672"/>
        <property type="gene ID" value="AFR_11915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2426"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="24543" checksum="E31E1AF61506C348" modified="2014-01-22" version="1">MATELSGRTALITGGNSGIGRATADALAAQGATVVLTGRDAARGEQAVKRIEQAGGTAHFIAADLHDEASARALAAQATGVTGQIDILVNSAGIFPFGPTEQTTEEAFDDVFAVNVKAPYFLVAELAPAMAARGQGAIVNVTTMVAEFGASGMGLYGASKAALVLLTKSWAAEFGPSGVRVNAVSPGPTRTEGTAAMGEALDQLAAAGPAGRPGGPEEVAAAIAFLAGDAASFIHGAILPADGGRIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVD3</accession>
    <name>U5VVD3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359107.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06435"/>
    <dbReference type="EnsemblBacteria" id="AGZ39576">
        <property type="protein sequence ID" value="AGZ39576"/>
        <property type="gene ID" value="AFR_06435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1305"/>
    <dbReference type="eggNOG" id="COG3217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028286_0_1_11"/>
    <dbReference type="OrthoDB" id="1885467at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOSC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="109"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="29076" checksum="E67E4E328CEE1F58" modified="2014-01-22" version="1">MRISSLHTYPVKGGHRLDHDAALVEPWGLAGDRRWMIVDQDGNGITQRQAPILTGLRPVPYAGGLDLNGFRVDEPVDGPKATIRVFSSKPPLTARIAPAATDFISTFLARPARLAWLADTGVRPIETHARPSDRVSFADGYPLLLTTTASLDALNDWLLEAGDEPVPMTRFRPNVVISGTRPWAEDNWLGGRLQLGDVTFRAAKSCSRCVVTTIDQDTGETGRQPLRALGRHRRYTDGLLFGINLIPDITPGETGIIRAGESSLALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVL1</accession>
    <name>U5VVL1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Single-stranded DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_07280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359276.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07280"/>
    <dbReference type="EnsemblBacteria" id="AGZ39745">
        <property type="protein sequence ID" value="AGZ39745"/>
        <property type="gene ID" value="AFR_07280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1486"/>
    <dbReference type="eggNOG" id="COG0629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078758_1_2_11"/>
    <dbReference type="OrthoDB" id="1942216at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="128"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="177"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="240" mass="25809" checksum="8FAAEFB943869914" modified="2014-01-22" version="1">MFETNVVVVGNVLTAPEWRRPANTNALVANFRVASTVRRLDRETGRWVDGNQFRVRVNCWRRLAEGVASSITVGDPVIVSGRLSTRDWTDAEGNLRTSYEMEAVSVGHDLARGRGRFFRTKAVGATSAVETPEADGQVRGESSVPVSEDEAPTVYGDGILDGDEPTFEDVPAVAGLRGGSIEPFDTEMVMPSTDEPGPADEPKPSEEPEVEISVEAIAGESGGRRSTGRGRGSRRQPVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWC8</accession>
    <name>U5VWC8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361218.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41159">
        <property type="protein sequence ID" value="AGZ41159"/>
        <property type="gene ID" value="AFR_14385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2919"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_2_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="20841" checksum="76A342A44287DE1A" modified="2014-01-22" version="1">MGRWEPDTRGRLLHAALELFADQGYDATTAAQIAERAGLTKTTLFRHFADKREVLFQGQPALVAVAIAGVESAPEGATALDLLRAGLLALCEAHVPEQQEIGRQIDRLIAASPELKERAIFKRSAIAAALETTLATRLGDRRLAGVLADLGVRAYYEGFSLWSDTRRDDKLADVVADELAAYESVLPQVTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWI2</accession>
    <name>U5VWI2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative multicopper oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_14710</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361283.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14710"/>
    <dbReference type="EnsemblBacteria" id="AGZ41224">
        <property type="protein sequence ID" value="AGZ41224"/>
        <property type="gene ID" value="AFR_14710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2987"/>
    <dbReference type="eggNOG" id="COG2132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009100_6_2_11"/>
    <dbReference type="OrthoDB" id="971126at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="PTHR11709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="201"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="491"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="604" mass="64023" checksum="DD213267BFE47F39" modified="2014-01-22" version="1">MQAILILIDLTLAVLTLIAAPVAGIRRTRLWLISLAALAAGRLAVACLMLTGGLLLADSRLAVQVPLALLPALWAVWRPGRTAAHLSAAGAMLSVWWLLAPFAPQDTVLVLAGSVLALAGIGGLSVAVGRWRESRSRLSRLPWLTTAFLLVPAVTLVVAGQANASATDHDHGGAGQLSVDQLVGPRNQVPDVRTTLVAARSQVRLASGRTVEALTFNGSSPGPEIRVKKGRLIEVTLVNTDVAEGVTVHWHGVDVPNAEDGVPGVTQDAVPPGGRHVYRFTATRAGTFWYHTHRDGLLNVKKGLYGALIVEDTAPFTGTERTVFTHAWPGSGGDVTAFDRDDRPAPAQLPAGRNVLLRMINSSEEPQTLQVRGTPFQVAAIDGNTVEGATPLQPGTELLLAAGGRYDLSFTMPAGPATVALAGSPDVALTFSPDGAAGPAPAADGGQFDPLTYGSGTPPAAAPYQRTFDLRLDDGFGFSQGRLSYVSSLINGRLYPAVPSLEVERGDRVRVRLASRSLINHPFHLHGHRVRVLSRNGVPATGSPWWTDTLNVGSGELYEIEFIADNPGIWMDHCHNFQHGANGMIMHLAYRSVSTPYSSDHAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX15</accession>
    <name>U5VX15_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Respiratory burst oxidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_09855</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09855"/>
    <dbReference type="EnsemblBacteria" id="AGZ40260">
        <property type="protein sequence ID" value="AGZ40260"/>
        <property type="gene ID" value="AFR_09855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2010"/>
    <dbReference type="eggNOG" id="COG4097">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_19_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="215"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="449" mass="49092" checksum="0CF766D3FEF41C46" modified="2014-01-22" version="1">MSPTPRWWAGAAGTAAGLSLVVVTALWTANEGLSEVARGGWTGAGAAGRLAALWSADLMLLQVVLMARIPLVERAFGQDKLARWHRWTGFTSFQLLLVHIVLATLSYAQGERVVAQFVEFVADYPGMLLATAATAALVLVVVTSVRRARRRLRYESWHLLHLYAYLGVALALPHEIWTGRDFTGSPAARAYWCGLYAAAAGSVLIFRLGMPLWRTARHRLVVDHVVPEAHGLVSVYLRGRRLDRLPVRAGQFFVWRFLDGPGWSRGNPFSLSGPPNGDQLRITVKGLGDGSSRVSTLRAGTKVLIEGPYGRLTAESYRGGPVTMLACGVGITPLLALLWDLPYGHGQATLVYRARRAEEVAFLDEIEWLADQRGVRLVPLIGPRAHQASWLPAEYADYSDAEALREIAPDIAGHDLYLCGPDPWTAAVRTAARAAGVPAAQLHRERFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXE8</accession>
    <name>U5VXE8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MATE family efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361749.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16800"/>
    <dbReference type="EnsemblBacteria" id="AGZ41638">
        <property type="protein sequence ID" value="AGZ41638"/>
        <property type="gene ID" value="AFR_16800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3408"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_0_1_11"/>
    <dbReference type="OrthoDB" id="216729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="479" mass="49041" checksum="DEFBD607A1EC1C6F" modified="2014-01-22" version="1">MSTANLDNPATAAAVGTNRWYLSAAPITRALVHLCLPMAAAMIVGAVYNLINAGFIGSLHDTALLAAVTLGAPILGLVMAVGNVFGVGGGALVSRLLGAAEHDPARAGEIKHVSSFAFWGSVLVGVVIGGAGLLLLHPLVALLGADAAAVPATNAFVGVMLAFVPVLAASVCLEQLVRAEGAARQVMIGLIASTVANLVLDVLFILVLHWGVAGAALATGLANLVVIGYFAFWLQRNSEHIRLAPRWFTLSPAVLKPVLGVGVGTLLQSAFLIVTSLVLNNLAAAYGDGPLAAMGVAVRIAQVPEFLIMGVTFGVLPLLAYSYGKGDGGRLAAALRGSAVTVGGIALIFSVVVFVFREQLFSAFVADPSVLSIGVTIMTAQLVAMIANGFTGLITSLFQAAGLAMAATVMSVTQGVLFIPVVLLGNLWFGLPGIIWALTVTEGAVLVAGAVLWLAYRRSIDRGLAEGSPERAEEALEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXM0</accession>
    <name>U5VXM0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquinone/menaquinone biosynthesis methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360394.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10955"/>
    <dbReference type="EnsemblBacteria" id="AGZ40480">
        <property type="protein sequence ID" value="AGZ40480"/>
        <property type="gene ID" value="AFR_10955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2231"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115249_0_0_11"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="39"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="180" mass="19957" checksum="36C14A2B84750287" modified="2014-01-22" version="1">MKFAQADRYDQHSHRRLTRLRSRILDDIAAADLAPGSRVLDVGTGPGRLPLAIAATFPDLRVDGVDLAPEMISLARRLAADTSVTFTVGDVARLPFPDETFDLIVSSLSQHHWADVDGGIRDLHRVLRPGGRLWIYDVRWGLFRATAAARSSFPPESVTRVPVRPIRLPINFMARLSARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXN6</accession>
    <name>U5VXN6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fumarylacetoacetate (FAA) hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360301.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10710"/>
    <dbReference type="EnsemblBacteria" id="AGZ40431">
        <property type="protein sequence ID" value="AGZ40431"/>
        <property type="gene ID" value="AFR_10710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2183"/>
    <dbReference type="eggNOG" id="COG0179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_3_4_11"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="71"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="30508" checksum="E9F7B1B54AAFD300" modified="2014-01-22" version="1">MKLMRIGDAGAERPVVRVDESTYVDVADLGDQFFTAPDDFRSTVDARITAGQVASFAGERIGAPIPRPHQILCIGLNYRDHAAETGQAVPDEPILFTKSPNTLVGPHDDVRQPRGSTKLDWEVELGIVIGRRASYLDDHNAAREAIAGWVVTNDISERAFQTERGGQWSKGKSAETFNPAGPWLVTPDEIPDVNALGMWLDVNGVRRQTGSTSTMVFDPYEIVRYLSQFLVLEPGDLINTGTPPGVGMGFKPPIWLQPGDVMELGIDGLGSQRQTVVAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXZ5</accession>
    <name>U5VXZ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360550.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40565">
        <property type="protein sequence ID" value="AGZ40565"/>
        <property type="gene ID" value="AFR_11380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2319"/>
    <dbReference type="eggNOG" id="ENOG502ZT9Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2452873_0_0_11"/>
    <dbReference type="OrthoDB" id="1899431at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="89" mass="9756" checksum="9DA0D8B28ADD66E4" modified="2014-01-22" version="1">MIEPHITLDPASLGPVEEKLRGPLEDQLTSALQNATDTVSHDYAGEPVDDVQVKLLEQTRAGLHPDIAEGFNPDQTELRRVAEAIIDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY51</accession>
    <name>U5VY51_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17705</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362111.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41819">
        <property type="protein sequence ID" value="AGZ41819"/>
        <property type="gene ID" value="AFR_17705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3589"/>
    <dbReference type="HOGENOM" id="CLU_793727_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="349" mass="36043" checksum="46B53920549B7C7E" modified="2014-01-22" version="1">MTEQDALARLAAANPVPAEEVRDAAASSARAQFLLRTVLSRPRRRFAVTSRRRLRWALLPAGLVAAAVAAVAVLQTPAPPAPDPPVVTSAMVLNAAAEQAGQDAGRARFVHVTGTAGRVVHLDSAGGYDVIRVDAVRSVQPAGGLPGEGWLTIGEAGTSVRPLTAADAAAYARDGAPDAAALPRDPAGGLHPDLAGDHGFEGDVAALPDDPAQTGSAMIAWLAEQGLPVPPDPQGWLFRTGTGLLDTFTSVAGSKERAKIYRMLAGLTGVSSLGPAADPLGRPSRGLAYTERTTRFGLIEWQVYLGADSDLIMYSQAVVRQPGPANASLSPGAVQYSTAVTSVAWSDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYD2</accession>
    <name>U5VYD2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18105"/>
    <dbReference type="EnsemblBacteria" id="AGZ41899">
        <property type="protein sequence ID" value="AGZ41899"/>
        <property type="gene ID" value="AFR_18105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3674"/>
    <dbReference type="eggNOG" id="ENOG50320W7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1958305_0_0_11"/>
    <dbReference type="OrthoDB" id="1705089at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="12650" checksum="2B1B760B388B0D98" modified="2014-01-22" version="1">MLVLDLLSVTEGVNVSGDQAGFHALAVLVAGGGELALSDEGQLLGVLVEATEGPVVAGLHDRRLTVRGGDEGRSILADELRAVGDMDDGGHAHIEYFEGHFYLGAGSLPLILISPHGGMPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYR0</accession>
    <name>U5VYR0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362402.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18765"/>
    <dbReference type="EnsemblBacteria" id="AGZ42029">
        <property type="protein sequence ID" value="AGZ42029"/>
        <property type="gene ID" value="AFR_18765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3811"/>
    <dbReference type="HOGENOM" id="CLU_873270_0_0_11"/>
    <dbReference type="OrthoDB" id="1134822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665501" evidence="1">
        <location>
            <begin position="25"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="32780" checksum="53D57999DBDFBBE3" modified="2014-01-22" version="1">MARWTAIAIGGAAALIAPAGAAHAAEQATLTLQAPASVARAGSLVVTGTFSAGPASLTVTRKDLTGSHTLPAITADAGGAFTLRDKPMIGGTNTYTVVFAGDAGHDPTNQQVSVAVSRRATKVTITTDASTYRYAATAKITAKLGTTHDSRTLCVYAQPAGGDKKKIKCGSGTVTATYKPTRRTVLSASFAGDQWYAPLTASRTVKAGAKIEEVLVGQYATSGKYKLYRTSVDPALAVRVSPAHPGGCIGFTAEQYRSGKWRTLATAKCFRLDGDSIGGGTLLGSHSSGTTYRLRASFAGDSTSGAATGAWQYLKFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYT4</accession>
    <name>U5VYT4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Retinol-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362320.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18345"/>
    <dbReference type="EnsemblBacteria" id="AGZ41947">
        <property type="protein sequence ID" value="AGZ41947"/>
        <property type="gene ID" value="AFR_18345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3723"/>
    <dbReference type="eggNOG" id="COG0793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044498_0_0_11"/>
    <dbReference type="OrthoDB" id="1646508at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TSPc" evidence="1">
        <location>
            <begin position="69"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="34417" checksum="0F9EDD14F429DB9D" modified="2014-01-22" version="1">MSTVIDDALALLTTRYIFPEKAQAAAVAIRRRQEAGEYDGLDEEKLAERLTAELFEVCADKHLRVRTRPPEMHASLTEAELITAYKESVRTANYGIARVERLDGNIGYLDLRQIADASVGGRAIAAAMELVSHTEALILDLRRNRGGSPNGVTFWLSYLFADDMTHLNNIYDGESGGTRQFWSLAYLPGERYPDRPVWVLISDVTFSAGEEFCYNLQVLERATLIGRTTRGGAHPTQFFPLTPTLEITVPTARSVNPMTGGNWEGTGVVPDVDVPAEEAFDIAYRRALDHVLTTTAPATVLAEARAALEDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYY8</accession>
    <name>U5VYY8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18595</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18595"/>
    <dbReference type="EnsemblBacteria" id="AGZ41997">
        <property type="protein sequence ID" value="AGZ41997"/>
        <property type="gene ID" value="AFR_18595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3774"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_25_6_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011075">
        <property type="entry name" value="TetR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16859">
        <property type="entry name" value="TetR_C_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="22108" checksum="DB6EF633B3F74C29" modified="2014-01-22" version="1">MTTVSLDDAADQERKAPGRPRNARADAAIIEAVLELLSAGQSADALSMEAVAAKAGVGKATVYRRWPNKEALLIDAIRTMKGPPPVPAGESVREDLVMLIAGMRNKRMEHYGKVTACLVPELLKSPEMYTIYQSAMEPRRDVMRQVLVRGVANGDLRADLDIELTMLMLSGPTIAQNMLRWNPNVPDQGFAEALVDAVLRGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYZ8</accession>
    <name>U5VYZ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_13075</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360960.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13075"/>
    <dbReference type="EnsemblBacteria" id="AGZ40901">
        <property type="protein sequence ID" value="AGZ40901"/>
        <property type="gene ID" value="AFR_13075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2655"/>
    <dbReference type="eggNOG" id="COG1129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_92_3_11"/>
    <dbReference type="OrthoDB" id="551294at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="10"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="247"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="495" mass="52382" checksum="E8C68D46987C4F9F" modified="2014-01-22" version="1">MTAGPGRALLRIEGLTKSFGATRALDDVSVTIHSGEVLAVVGHNGSGKSTLVKIMAGFHTADHGTIDPRSEVGPPTELRFIHQDLGLVETLSTRENLGLGSGRDGPPWLPLRKAAERRLARELTAQFGVDLDVDALVQQLSPAERTIVAIARALRGWDQDRNMLLVLDEPTAALHGAEVDRLMAVVRRVAERGAGVLFISHRLDEVLGVADTVLALRNGRVVGNVRGAEVDYAGLVELVAGGSVAALDRRDTGTGDPVLRVRGLAGRSIVDLDVTVDAGEIVGVAGILGSGRDEVCSLIFGGRRRSSGTVELADHPLAPGDLQESIRHGAAFVPGDRHQDGAVMTMTATENLMAVRIPGTESRWRRLRKKHEADEAAKWFATCGVVPAQPSMDLSQFSGGNQQKVVLAKWLRTRPRLLLLEEPTQGVDVGAKAVIHRLIAETAGAGTGVLVASSEAKELAELCDRVLVLADGVVTAVLAGAELTETRILQASVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZB0</accession>
    <name>U5VZB0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative 17.2 kDa protein in melC2-rnhH intergenic region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361080.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13675"/>
    <dbReference type="EnsemblBacteria" id="AGZ41021">
        <property type="protein sequence ID" value="AGZ41021"/>
        <property type="gene ID" value="AFR_13675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2777"/>
    <dbReference type="eggNOG" id="COG5637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079860_2_1_11"/>
    <dbReference type="OrthoDB" id="1719670at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polyketide_cyc" evidence="2">
        <location>
            <begin position="10"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="180" mass="19809" checksum="F53661ADC2E88ADB" modified="2014-01-22" version="1">MDLTATTTIRRPASEVYAFWRDLENLPTFMAHLEQVRTTGDRTSHWAAAAPFGTDVEWDAEIIEETPGEKIAWRSTGDADVPNAGTVRFVPAPDGVSTEVHVVIVYDIPGGAVGKAVAKYFGEEPHQQLDDDLRRLKQVLETGEVVRSDGAPWGKRARKEFPQRPAQPLSDAELEKGADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZC6</accession>
    <name>U5VZC6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integral membrane sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20380"/>
    <dbReference type="EnsemblBacteria" id="AGZ42348">
        <property type="protein sequence ID" value="AGZ42348"/>
        <property type="gene ID" value="AFR_20380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4132"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="192"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="375" mass="38633" checksum="4C8E3961CAB747B1" modified="2014-01-22" version="1">MVRGLRAPIRGATYRRGAFLLLGGALLLPYGLVAVGLARQVRDGGTPLAVVVPMTIVAAAIGLVPPLLRGARSLEIAAVRGLLDVDLPEPPERISAEARLRAALWFAVHLTVGGGVGLALFVVVPTAAVLVTTRTGWAAAGPAALSVAVLVATVYAVAGLGALARLMAPVLLGPSADERVAALEAEARRLAERNRLARDLHDSVGHALTVAVLQAGAAGQLLATDPAFVARALTAIEETGRAAMEDLDRVLGVLRDDLPSEPDGTRPDLGDLAALAHRTGAGLATAGPLLAVPPALSQEAYRIVQEALTNALRHAGATPVSLDVTAAGTSLVVEVRNTLRGRGLAGMRERVTLLGGDLTAGPDGDQWRVRAELHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZD2</accession>
    <name>U5VZD2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulatory protein TetR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361114.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13845"/>
    <dbReference type="EnsemblBacteria" id="AGZ41055">
        <property type="protein sequence ID" value="AGZ41055"/>
        <property type="gene ID" value="AFR_13845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2811"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_25_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011075">
        <property type="entry name" value="TetR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16859">
        <property type="entry name" value="TetR_C_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="19036" checksum="DBFE94B669833BEA" modified="2014-01-22" version="1">MLTAIYTAVGELMGEGADKISFPVIAERAGVNPTTLYRRWADVNALLEDVAVAALTRNGESIPDTGSLEQDLMLWADIIARDITRPERVRYLRSMVAARVDVVAHCPITETRCEQAAEMVRRAAERGETAPTVPQILDHIIAPLYHHVVFALPVDHKYARRLVHDVLAMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZJ8</accession>
    <name>U5VZJ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362569.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42197">
        <property type="protein sequence ID" value="AGZ42197"/>
        <property type="gene ID" value="AFR_19625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3983"/>
    <dbReference type="eggNOG" id="ENOG5033BFR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161288_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="78" mass="7994" checksum="33DAC5E4EC19FEA4" modified="2014-01-22" version="1">MNSKWSIRSENSVLSICSKDSVLSIGSVGSGLSIGSVGSFASVASVGSVLCVGSLLSAVSLWSVMSWRSRKAVTAGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZQ2</accession>
    <name>U5VZQ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amidohydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362619.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19875"/>
    <dbReference type="EnsemblBacteria" id="AGZ42247">
        <property type="protein sequence ID" value="AGZ42247"/>
        <property type="gene ID" value="AFR_19875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4033"/>
    <dbReference type="eggNOG" id="COG2159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044590_0_0_11"/>
    <dbReference type="OrthoDB" id="1832218at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="PTHR21240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="19"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="290" mass="31754" checksum="F0F98C15CFF09BE6" modified="2014-01-22" version="1">MIGDVTEFWTELGLPGLADVHTHFLPERMQRRVWAHYDQAGPLIGRPWPIHYRLPEAGRVAELGRLGVRIFTALAYAHRPGMAADLTAWTLDFAGRTPGCLPSATFFPEPSATGEVLSALAHGARIFKLHLQVGGFDPRDDLLDDAWGVLAESGTPVVVHAGSGPVANGFTGPGPIGDVLRRHPRLALIFAHLGGPEYEGFLDLSERYEHARLDTTMAFTDFFEELAPFPPGLRPRLRDLGLAGKVLLGSDFPSIPHPYAHQLEALARLDLGDDWLRAVCWHNAARLFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZV5</accession>
    <name>U5VZV5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362791.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20735"/>
    <dbReference type="EnsemblBacteria" id="AGZ42419">
        <property type="protein sequence ID" value="AGZ42419"/>
        <property type="gene ID" value="AFR_20735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4204"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082655_0_0_11"/>
    <dbReference type="OrthoDB" id="1007505at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="21"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="31956" checksum="47247799FCEA572D" modified="2014-01-22" version="1">MKIALDPYMLRKVPLLSLPRVVADLGYHHIELSPRDDFLPFFLHPRASSADVAAFKGALAEAEVSVASVLPLYKWSGPDEDERQAAVRYWKRAIEITVDLGVDVMNSEFNGRPEAAPASEAQFWRSMEELLPIFEREGVRLVLEPHPDDFVEDGIAGVNLVKAINSPLVTFLYCAPHTFHQGGDLAAVIRHAGPLLTQLHLADSFDHRGSSGLRYIVNPPGSTVRVHQHLDIGQGEVDWNLFFRTLAEVGFDGTATVCVFAWEERAEESCRFNLDQVNQYVAKHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0B1</accession>
    <name>U5W0B1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative AraC-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362844.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21000"/>
    <dbReference type="EnsemblBacteria" id="AGZ42472">
        <property type="protein sequence ID" value="AGZ42472"/>
        <property type="gene ID" value="AFR_21000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4257"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047930_0_1_11"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035418">
        <property type="entry name" value="AraC-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14525">
        <property type="entry name" value="AraC_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="238"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="341" mass="36866" checksum="FA276FA84984A308" modified="2014-01-22" version="1">MDTAGAAVQDTEPATPDGDVAVCATLRTTDLDEARDFCRRMYYGPLEVKPAGSIDGFAFTGEVVQIGPVTIGEISYGTEICLASGDLETAYHVLAPLTGGVHVLHRGAATKADPSQAAVFRPIGDIDLRWSADCRLISVKVDRLALERELDAALDRRVGSPLPLGATFGLAGGPGRSWLALVRLLHAEARQPDGLTLFPHLLRQWRDLVVSGLAQAVEHPFSQNPAGRPTARRPRTVKRTLDAMHAEPGRSFTAAELAGIAGVGIRVLQESFRQHVGVPPLTYLRRLRLEGVHAELSRAGPDQISVSEVATRWGFTHLGRFAGAYRERYGETPSQTLRDQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0D8</accession>
    <name>U5W0D8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_22065</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363054.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22065"/>
    <dbReference type="EnsemblBacteria" id="AGZ42683">
        <property type="protein sequence ID" value="AGZ42683"/>
        <property type="gene ID" value="AFR_22065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4472"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DUF4162"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DUF4162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="32349" checksum="F383AE57A06D3FFA" modified="2014-01-22" version="1">MITATGLRKAYGDHTVLDGIDLDVPAGSVFALLGPNGAGKTTAVQILTTLKSADAGEIRVGGHDPATEPDAVRAAIGVTGQFSAVDALLTGRENLLLMADLHHLGRRAGRRRADELLVQFDLVDAAGKQVAAYSGGMKRRLDIAMGLVGDPRIVFLDEPTTGLDPRSRQAMWQTVRSLVAGGVTIFLTTQYLEEADQLADKIAVLDGGRIVAEGTAEQLKQLVPGGHVTLRFAEVQALRLAATALDVTEVDADQLTLDVPGDGSVRSLRALFATLDDARVEVDSLTVHTPDLDDVFLALTEKEPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1M9</accession>
    <name>U5W1M9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23310"/>
    <dbReference type="EnsemblBacteria" id="AGZ42932">
        <property type="protein sequence ID" value="AGZ42932"/>
        <property type="gene ID" value="AFR_23310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4724"/>
    <dbReference type="eggNOG" id="COG4671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050684_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_tran_28_C" evidence="2">
        <location>
            <begin position="224"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="34096" checksum="A3FE39AE5D5A6B14" modified="2014-01-22" version="1">MTGWYVHHHGAGHLTRFRAVRPHLAGEVVVFSSLPEPSSLPPQTSWVHLGRDDDRPAGAPDPADSDPTAGGLLHWAPLGHAGHARRLAVITDAIATRRFDRFVIDVSVEVALLVRLLGVATVVVTQPGDRSDDPHRLAYASATRVLAPWPGGAHRSQGFVEVGGISRFDGRVRDAEPDPGTVLVLGGGIVEGDLTERVARAAAASPGTRWQVAGGPTWIDDPWLALQRAEVVVAAAGQNSIADLAAAGARAVVVPQPRPFDEQAATGRILLDRSLAVVEPAWPADDAWPAVLDRARSLQPDWSLWGVAGAARRAADVIAEVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2B0</accession>
    <name>U5W2B0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362482.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19190"/>
    <dbReference type="EnsemblBacteria" id="AGZ42110">
        <property type="protein sequence ID" value="AGZ42110"/>
        <property type="gene ID" value="AFR_19190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3898"/>
    <dbReference type="eggNOG" id="COG2039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048887_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="SSF53182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666016" evidence="2">
        <location>
            <begin position="23"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="417" mass="44711" checksum="027CAE3CB8F5CD64" modified="2014-01-22" version="1">MRLFTVLAVSAALLAVPQAASASGSCYDRAAPITYEESRIETPALPGGTRVPYTLVEAGGFTAYAEGLSGALCRTRSVAAAKLLLQRRGAQLWSTAVQRAQGKVQQGTIERYDDRPLYWTRLLGTRDLRQWQPAFPVTDAERASLIKTFEYASRGVSSTTFGRGVKRVLVSGFDPYQLNGEIRRSNPSGASALQLDGLRFGDVLVQAVVLPVTWSGFDAGIVEDTFGPHLTARSRDRADLIMTISQGNRGLMNIEQWAGAWRGGSPDNNNEGVAEPVPSAPRWPQPDPQPEFIETTLPFEKMIAAGTGPWPTALNPRLCEWPAGTRPDPAAVRCHTNGPTPGSQAQSGGGGNYLSNESMYRSNRLRLALGATDVPGGHLHISALVYPADPLVIIDDAFSADRAATIEQTVALVKAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2F2</accession>
    <name>U5W2F2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PadR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362527.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19415"/>
    <dbReference type="EnsemblBacteria" id="AGZ42155">
        <property type="protein sequence ID" value="AGZ42155"/>
        <property type="gene ID" value="AFR_19415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3941"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_3_2_11"/>
    <dbReference type="OrthoDB" id="1706427at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="25"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="111" mass="12551" checksum="6CDCC816F3C13FB5" modified="2014-01-22" version="1">MGDDDLLATHLQELRRGTVVLACLLLLEQPGYGYALLDLLERRGFAVDANTLYPLLRRLEKQGLLTSEWNTDEARPRKFYRTSAEGTALAGDLSRDWDRLDTALRTLKGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2L6</accession>
    <name>U5W2L6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43473">
        <property type="protein sequence ID" value="AGZ43473"/>
        <property type="gene ID" value="AFR_26045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26045"/>
    <dbReference type="HOGENOM" id="CLU_2839799_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="65" mass="6839" checksum="649082F295236C71" modified="2014-01-22" version="1">MKLISMSLPLGAHCERHGSRWVGSPERLKPMGWLAITSLSLSPAAGMNQPSSGTPYADGKVISSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2T0</accession>
    <name>U5W2T0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mannitol dehydrogenase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25255"/>
    <dbReference type="EnsemblBacteria" id="AGZ43317">
        <property type="protein sequence ID" value="AGZ43317"/>
        <property type="gene ID" value="AFR_25255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5118"/>
    <dbReference type="eggNOG" id="COG0246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027324_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019594">
        <property type="term" value="P:mannitol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000669">
        <property type="entry name" value="Mannitol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013118">
        <property type="entry name" value="Mannitol_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023027">
        <property type="entry name" value="Mannitol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013131">
        <property type="entry name" value="Mannitol_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01232">
        <property type="entry name" value="Mannitol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08125">
        <property type="entry name" value="Mannitol_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00084">
        <property type="entry name" value="MTLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00974">
        <property type="entry name" value="MANNITOL_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mannitol_dh" evidence="1">
        <location>
            <begin position="31"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Mannitol_dh_C" evidence="2">
        <location>
            <begin position="221"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="494" mass="51346" checksum="E26E7E85FC65F27D" modified="2014-01-22" version="1">MTTTPLLGLGTLGRLPEHSRPLLEPGSVPAGIVHLGLGAFHRAHQAVYTEEAVAAGGGDWGIVGVSPRSSAVVDDLLAQDRLFSVTTLSAAGPHTRVVGALAEVRHAASDPAGVVALLADPAVRVVTLTVTEKAHRLDPVTGELTVDAALLADLAGAGPSVTIPGLLVRGLLARHRADAGPIALVSCDNMPSNGRRLHGMVEQARVLVPGAAPAFGDWVRESVTFPGTMVDRIVPASTAETLAIAEAALGARDLAGVHGEPYRQWVIEDRFPGGRPAWELAGAVLTGDAGPWERLKLRALNGVHSAIAYLGALAGRDTIVTALEIPGLRTAMERFIAEDVATSFEPPPGVSVVGYGASVLERFANPAIAHATRQVAMDGSQKLPQRVLHTILDRRAAGASPRWAALVVAAWMRFVQGRTDDGRELPLNDPLAAQIRTRLAAAPATPQGVADALFGLDAVFPAELAADATVRALVVDWLTALDRHGVEATLAGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2W6</accession>
    <name>U5W2W6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_20315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362707.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20315"/>
    <dbReference type="EnsemblBacteria" id="AGZ42335">
        <property type="protein sequence ID" value="AGZ42335"/>
        <property type="gene ID" value="AFR_20315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4119"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="222" mass="24642" checksum="C4E03B459121FBC2" modified="2014-01-22" version="1">MRLLVVEDEARLAAALQRGLQAEGFAVDVTGDGQDGLEMARHGEYDAMILDVMLPRLSGYRVVRQLRAEEHWLPVLMLSAKDGEYDQADGLDCGADDYLTKPFSYVVLLARLRALLRRGTPERPAVLRHGDLELDPAQRKVTVAGAEVVLTAREFALLEYLLRRPGEVVSKTELLDHVWDAALDTAANAVEVYAGYLRRKIGRERLETVRGAGYRLVAIDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Z7</accession>
    <name>U5W2Z7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_26210</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364269.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26210"/>
    <dbReference type="EnsemblBacteria" id="AGZ43504">
        <property type="protein sequence ID" value="AGZ43504"/>
        <property type="gene ID" value="AFR_26210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5312"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012341_2_1_11"/>
    <dbReference type="OrthoDB" id="856767at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666035" evidence="7">
        <location>
            <begin position="38"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="644"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="697"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="740"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="8">
        <location>
            <begin position="272"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="767" mass="77042" checksum="71F0807FB19E0400" modified="2014-01-22" version="1">MSLAWSMVRHRFAAFAGTFVAIALGVAVVAGSTTIWASSQPQTPARLAAAPVLVHSPSVGDTESGYPEYRSWTTAEATGLAQRLAAVPGVTAAIADPSFYVQRVVAGRATGDPEAARTEGHAWSSAVLGGYRLSAGAAPARPGEVALAGAAPGSRIEVLTARGPATWTVTGTTDGPGLYVADTAATALASGVRVIGLTVSGEPESIAAAARTAVGADGTVLSGSARDALEPESVTRIRWLGAQLLIAMVFLGTFVAVFVVASTCALSAAQRRREIGLLRAVGATGGQVIRMMYAETLVVALLGGVAGVVLGAVTAPLLAAPLVSAGLEPAGFTVTLQPVALGAAFVLGIVVALIGVWAAARRSSRVPALDALREAAIEPRPMTLLRWIAGPLAAAGGGALLVALPNLDAQAQSSATLGGAMLLLVGAALLAPVVIGPLVRLGTWPWRHGASGMLLREGTLTGTRRVASTAAPVLLAVGFAVLFTGTVATISDSAGVDAAAEVPAALVAAPDGTPGLSQAAVTGQPGDARFPTRLLVTRDGKTTGYDAVGTDQVDGVVTSTDLGPTLDVRFADGTTASLPVTAVRDDAPAPILLPRELVLRHDPGALAEAVLLTGPAAPAAGLQVLSARDYVQIEIDEEGDIIDIFLVVLLGLGVGYTGLAVANTLLMATAARRTEFRALRLAGGGTGHVLRVTSGEAFLAVVTGTLLGGSVSLICLLAARRSIERELGQAIDLVVPWGQTAVVVAVCAVVAVLAAGAPVLRAGAGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3B5</accession>
    <name>U5W3B5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_20770</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362798.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20770"/>
    <dbReference type="EnsemblBacteria" id="AGZ42426">
        <property type="protein sequence ID" value="AGZ42426"/>
        <property type="gene ID" value="AFR_20770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4211"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="OrthoDB" id="664909at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="35431" checksum="EF76F8EDD4012193" modified="2014-01-22" version="1">MDQRPRLDDVAARVGVSTASVSLVLRGAPGPSAETRRRVLEAAAELGYRADRTASLLARRRRHLLGVMLDIRSQFHAELVEEIQAEADRVGYEIVLSTLTRHRDEDRAVETLLDFRCEALLLIGPDAADDRLAALAAQVPVVAIGRRVAAGDVDVVRSADQVGVSEALDHLIGLGHRHIALVDGGRGVVAAARRRGYRDGMRRAGLADEIRIVPGDHTEEGGIRAGRRLAAASDRPTAVVASNDRCAVGLMDAFLRGGLDVPGQISVVGYDDSTLARLAHIDLTTINQDPAAQASHAVLAAVQRLDEGRKQRLEIVLNPRLVVRGSTAAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3U9</accession>
    <name>U5W3U9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560230.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28160"/>
    <dbReference type="EnsemblBacteria" id="AGZ43893">
        <property type="protein sequence ID" value="AGZ43893"/>
        <property type="gene ID" value="AFR_28160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5708"/>
    <dbReference type="HOGENOM" id="CLU_1933494_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="130" mass="14515" checksum="4863DE214D752C8F" modified="2014-01-22" version="1">MDRAARALIGSRPFPLSVRWGKALWHGELVFTEKGPAGTLWADRRSLRVRGDVHEWRVTGAGAFAEPDKIVFRLPDYQGRPVRLTCRFRDSHVTGAEVTCPELIAAPVEVSADELLQTIAAEVRRQHESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W445</accession>
    <name>U5W445_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363052.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22055"/>
    <dbReference type="EnsemblBacteria" id="AGZ42681">
        <property type="protein sequence ID" value="AGZ42681"/>
        <property type="gene ID" value="AFR_22055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4470"/>
    <dbReference type="eggNOG" id="COG4226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105073_0_0_11"/>
    <dbReference type="OrthoDB" id="1988756at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="17455" checksum="4FE869A2E187FD69" modified="2014-01-22" version="1">MDLTAYVERLREELAGAAELGGPEARALADRLTAPLESAFRLAMLDALSAAADEITQDLAPGSVEVRLRGGSPGFVVTPPPAEQATFEAAPPAAEPVEPDADTAVSRINFRLSDQLKSRIEEAAGRDGLSVNAWLVRAATTAVQTGGQTRTTRAPVGGQRYTGWAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4B2</accession>
    <name>U5W4B2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ANTAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_27980</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27980"/>
    <dbReference type="EnsemblBacteria" id="AGZ43857">
        <property type="protein sequence ID" value="AGZ43857"/>
        <property type="gene ID" value="AFR_27980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5672"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074354_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012074">
        <property type="entry name" value="GAF_ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036625">
        <property type="entry name" value="GAF_ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ANTAR" evidence="3">
        <location>
            <begin position="169"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="239" mass="25550" checksum="19863CFB3C20B052" modified="2014-01-22" version="1">MSMPSKTLDPHTAFAALARINLAETDLDGVLTHVAELAQRTVPGADDVSVTMVHRSTAETAVSTGDRALVLDEWQYREGLGPCLKAAAEKLTVSVPDLASDDRWPDWGAHAVSSGARSSLSVGLPIEDEIDGALNIYSTRRYAFGEDAILLAQTFAGYATVALSNAKRYGSTSDLVRDLQASMTSRAVIEQAKGIIMGRQRCSAEQAFTILTRIAQDRNRDVRDIATAVVARAEGRGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4W9</accession>
    <name>U5W4W9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">NagD-like Protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560595.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29985"/>
    <dbReference type="EnsemblBacteria" id="AGZ44258">
        <property type="protein sequence ID" value="AGZ44258"/>
        <property type="gene ID" value="AFR_29985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6073"/>
    <dbReference type="eggNOG" id="COG0647">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_1_0_11"/>
    <dbReference type="OrthoDB" id="1308416at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="33369" checksum="273013895531C664" modified="2014-01-22" version="1">MSLVETYDLVIFDLDGVIYLIDKPIDGAAEAVERLRSAGTAIAYATNNASRRAADVAALLEGMGVSAAAGEVLTSAGASAAMLAERLPAGAPVLVVGAEALRAEVRDAGLTVVDKLEDKPVAVVQGFGPDVGWKLLAEASLAVRAGALWMATNTDRTLPSPRGPLPGNGSLVGVLRTALNREPDVVVGKPAPALFETAASLSKSQRPLAVGDRLDTDIEGAVGAGMDSLLVLTGVSGPAELLQAPPERRPTFVSADLSGLFGDADAARIPVTADTLGGWQVADGKLTGSGTPVDALRLLTGVYWNGGEVKACSDEARDLLTGWGLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Z3</accession>
    <name>U5W4Z3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44204">
        <property type="protein sequence ID" value="AGZ44204"/>
        <property type="gene ID" value="AFR_29715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29715"/>
    <dbReference type="eggNOG" id="ENOG50327ME">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086897_0_0_11"/>
    <dbReference type="OrthoDB" id="2191469at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="25844" checksum="1B4B06C379A1A98F" modified="2014-01-22" version="1">MLGLLVASMVNCLGGEWWPMFAALGAVLIVVSVFVRPGRQPGTVFAPNFEVQPEEHRLLVASQERKTMSEVVEVVGRISATWSELDVMIDVVSAEHAVARATFDLAGLLERRERLRRTRDDLQTLPNGGLPASNPAVRSLTAQIDRINRAYSQVDAEISRRIAALEKTAEVGEMFVNEEALRRATQHAEQMLAELDQETLTSRLEPEPSTALADEMDAVLRAYRELIDIPNGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W549</accession>
    <name>U5W549_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29380"/>
    <dbReference type="EnsemblBacteria" id="AGZ44137">
        <property type="protein sequence ID" value="AGZ44137"/>
        <property type="gene ID" value="AFR_29380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5954"/>
    <dbReference type="eggNOG" id="COG1943">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3303261_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="SSF143422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Y1_Tnp" evidence="1">
        <location>
            <begin position="3"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="40" mass="4610" checksum="51441E3FE60810DE" modified="2014-01-22" version="1">MRHYYRVNKLWSGSYFAGSVGGALPSVVHQYIEQQNRPVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5H6</accession>
    <name>U5W5H6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative shikimate 5-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_30695</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30695"/>
    <dbReference type="EnsemblBacteria" id="AGZ44399">
        <property type="protein sequence ID" value="AGZ44399"/>
        <property type="gene ID" value="AFR_30695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6215"/>
    <dbReference type="eggNOG" id="COG0169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate_dh_N" evidence="3">
        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="SDH_C" evidence="4">
        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="27474" checksum="66A93811F86A94EA" modified="2014-01-22" version="1">MRKAAVCGKPIGHSLSPVIHTAGFAAAGLLSWTYTAIECAEAELPDLVAGLGPEWAGLSLTMPLKEAGLALASAASPVALATGAANTLVRRDDGWFADNTDVPGMVRVLRDAGLPQQPTVTVLGGGGTARAALAAAAAFGATEVTVVTRRPEARDELAPAASALGVTVLGREWSGAPAAFDADAIISTVPKGAADSLATAVHWRPGAVLFDAIYDPWPTPLAASAYTHGVRVVSGLDLLLAQALGQFEHFTGVPAPEEAMRAALTAAASVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5V1</accession>
    <name>U5W5V1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44543">
        <property type="protein sequence ID" value="AGZ44543"/>
        <property type="gene ID" value="AFR_31415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6358"/>
    <dbReference type="HOGENOM" id="CLU_2340498_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666112" evidence="1">
        <location>
            <begin position="23"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="10157" checksum="C91C499FA8FE10D0" modified="2014-01-22" version="1">MVSRIAVSLTATAALVLGTAGAAAAGSVTIGGCAVVTPTKGWVTQTVYVENYCGADRSFRVISVGPQLGFRCFTVASGQRGGWKFPTSKKYLWTEFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Y0</accession>
    <name>U5W5Y0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sensor histidine kinase yxjM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364085.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25730"/>
    <dbReference type="EnsemblBacteria" id="AGZ43410">
        <property type="protein sequence ID" value="AGZ43410"/>
        <property type="gene ID" value="AFR_25730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5216"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="181"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="390" mass="40923" checksum="600678FE51A1685C" modified="2014-01-22" version="1">MRRNLLLHVGQAGAVFFMAVLGLVDLNSGMGVGSSLDLAPVQVLSAVATAVVWLPAHRQGSTRLPKVAMLLAGFSLLLTIGIGVFGGGTGASFGSAEAVGLLGVLFVVTRRGDPNWAVPAVLLTGIAIGFLPLRGGLTTTYVIAGLALGLAATAVIALGVYLRTIESTRKRAMDSVRSEQRAEFARDLHDFIAHHVTGIVVQAQGARFIAEQDPKRVMLALEQIEAAGAETMASMRRMVGVLRDPNAQPDAPLAPLAGVDELGQLLESFTNQGDVLARLHVDGSTTGIPVEVSTSAYRVVMEALTNIRRHAQGARVADVWVRRTPDWLLVRVANDGAPPRAATPREAPGYGLVGLTERVRAVGGRITAGPGIDGGWVVDAALPLDQEVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5W604</accession>
    <name>U5W604_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25855</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43435">
        <property type="protein sequence ID" value="AGZ43435"/>
        <property type="gene ID" value="AFR_25855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5241"/>
    <dbReference type="eggNOG" id="ENOG5032DU7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1222620_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="25111" checksum="9AADBC5E2910A5D9" modified="2014-01-22" version="1">MRHNSTNRQAYLNLARLDLAEQLWAEVPDHEPDGRVPESAAVDVSSDYGRVTVLGHTIMEKNWDEISAEIDKLSSGEVNARTIKKHLADHIWCTLLVAVIQKVESLSRAVSFVSDGVKRFLQGALVERLGSAFSDGLRTAVAGIVVDKVWFALTKLLEVHFPFLGTDILRALRMLAVFSCPSIERHPEVYEHAVRPLLGDAGALISEDVKAQVSELFDAWGKRQGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6H2</accession>
    <name>U5W6H2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364391.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26515"/>
    <dbReference type="EnsemblBacteria" id="AGZ43565">
        <property type="protein sequence ID" value="AGZ43565"/>
        <property type="gene ID" value="AFR_26515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5375"/>
    <dbReference type="eggNOG" id="COG0421">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101666_0_0_11"/>
    <dbReference type="OrthoDB" id="1613081at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="237" mass="25658" checksum="66EC8332B7B65B9E" modified="2014-01-22" version="1">MSARFEELAWRETPIGEISLRRRLDPRLQVDVYEVKINDEFLMSSLFTVAEIELARLGLAALPAEARPDVVVGGLGLGYTARAALEDPRVASLLVVDAVGEVISWHERGLLPEAAALTTDPRSRLVEGDFFAMAAGDQGFDPDQPGRCFDAILLDVDHSPRHVLAPANAELYTVAGLRRLTAHLRPGGVFALWSDDPPDAEFEAVLAEVFEKSAAHVVTFPNPLTGGESANTVYVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6T5</accession>
    <name>U5W6T5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell division control-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561049.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32295"/>
    <dbReference type="EnsemblBacteria" id="AGZ44717">
        <property type="protein sequence ID" value="AGZ44717"/>
        <property type="gene ID" value="AFR_32295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6537"/>
    <dbReference type="eggNOG" id="COG0464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044189_0_0_11"/>
    <dbReference type="OrthoDB" id="585089at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045969">
        <property type="entry name" value="DUF5925"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19347">
        <property type="entry name" value="DUF5925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="198"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="40809" checksum="F206B70BA9DA0BEF" modified="2014-01-22" version="1">MRTPHQSVDRLASRTGDPAGASPLSASLPLSGSVDDGDRLVDVVDLLALDAFVTGREPYGRTTYLENVRTDAALTTDDARIVRDAVEEDGHGRLSVGEGWTLHVTRWKQSRRARVTVTAISAELAESVLAVAVDNAVEEPAPAADSVPIGFWHFGPHGPRRVQREIDAAPWDKIRTNYSDPVARTLERLMAIDGTAVNGRLLLLHGEPGTGKTTALRALAQQWRSWCQVDCVLDPERLFGDPAYLMSVALGSGDDDEPRWRLLMLEDCDELISGEAKAAAGQSLSRLLNLTDGLLGQGRNALIAITTNEDLASLHPAVVRPGRCLASIEVGRLPYAEAVSWLGTPAGVGPDGATLAELYALKTGARPVTVPMTSPTTGMYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6U7</accession>
    <name>U5W6U7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YggT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561265.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33350"/>
    <dbReference type="EnsemblBacteria" id="AGZ44928">
        <property type="protein sequence ID" value="AGZ44928"/>
        <property type="gene ID" value="AFR_33350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6750"/>
    <dbReference type="eggNOG" id="COG0762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136788_5_2_11"/>
    <dbReference type="OrthoDB" id="2069856at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003425">
        <property type="entry name" value="CCB3/YggT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02325">
        <property type="entry name" value="YGGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="92" mass="9693" checksum="DC87E3BCABCF68FB" modified="2014-01-22" version="1">MGLVLAIVSLVLLLMQVLLIARAVLDWTVALAGPTAYGSVRSRLLAGAHAVTEPVLAPVRRVLPPLRLGGMAIDLSFIVVFLAIVIIRSLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7G6</accession>
    <name>U5W7G6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561294.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33495"/>
    <dbReference type="EnsemblBacteria" id="AGZ44957">
        <property type="protein sequence ID" value="AGZ44957"/>
        <property type="gene ID" value="AFR_33495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6779"/>
    <dbReference type="HOGENOM" id="CLU_2476370_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666174" evidence="2">
        <location>
            <begin position="35"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="87" mass="8429" checksum="F294A9C11B1F6F69" modified="2014-01-22" version="1">MDRDKGLRRTTAITGALVAASLAGTLAVGLAARAAESTPQKPEAGTEAGTENSTGTDADNDSATTSTDSPQLSTGSDDSGQATSGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Z2</accession>
    <name>U5W7Z2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561674.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35410"/>
    <dbReference type="EnsemblBacteria" id="AGZ45338">
        <property type="protein sequence ID" value="AGZ45338"/>
        <property type="gene ID" value="AFR_35410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7165"/>
    <dbReference type="eggNOG" id="COG4326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057336_0_0_11"/>
    <dbReference type="OrthoDB" id="1033384at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009776">
        <property type="entry name" value="Spore_0_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40053">
        <property type="entry name" value="PTHR40053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07070">
        <property type="entry name" value="Spo0M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="29637" checksum="2D613D88FA1A7A50" modified="2014-01-22" version="1">MVFKRMMQAMGVGGPSVETVLANPNCRPGGFLEGQVHVMGGDHAVDIEYVAVGLMTRVEVESGDSEYNTDQEFHRQRLTGSFKLDAHARHDIPFRFDVPWETPITEVYGQHLHGMTMGLRTELEVARAVDKGDLDAVAVHPLPAQERILDAFLRLGFRFSRADVERGRVHGVHQTLPFYQEIEFYPPSQYAGRINQLEVTFIPTPHQLQVVLEIDKRGGLFTEGHDSFGRFDVDYHTVDQVDWTAQLNGWLQQSSQRRGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W812</accession>
    <name>U5W812_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Haloacetate dehalogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560393.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28975"/>
    <dbReference type="EnsemblBacteria" id="AGZ44056">
        <property type="protein sequence ID" value="AGZ44056"/>
        <property type="gene ID" value="AFR_28975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5871"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_1_11"/>
    <dbReference type="OrthoDB" id="1439833at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="26"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="33075" checksum="CD6C277DFF449C7D" modified="2014-01-22" version="1">MFDGFARHDLQTSRGPIFARVAGQGPPVLLLHGYPQTHVMWHTAADLLQKHFTVVVADLPGYGESFRPVPAADHTPHSKRALAVDLVQAMGLLGHERFAVAGHDRGGRVAYRMALDHPDRVTAAAVIDVVPTGDVWARADAKMALGYWHWAFLAQPAPLPERLIAADPDAFFDFHVRALGLGRAPDRYPADLMAGYRALLDDAGTVQAICEDYRAGAGVDREHDDADRGVRRIECPLLALWSANGALPRFYGDVLDVWRPWADDVTGQAMPASHFVVEDQPEATAEALTEFLLRKPSAFPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5W845</accession>
    <name>U5W845_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_29420</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29420"/>
    <dbReference type="EnsemblBacteria" id="AGZ44145">
        <property type="protein sequence ID" value="AGZ44145"/>
        <property type="gene ID" value="AFR_29420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5963"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="123"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="123"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="25069" checksum="0DD1A026E8D3676E" modified="2014-01-22" version="1">MLVAEDDESQAEVLRRYLEADGHHTVMVRDGQAALEQIRARQPELVVLDVMMPRLDGLNVCRILRDESDVPVLILTARASEDDVVIGLELGADDYLTKPYSPRELMARVRTLLRRGQRSPNPSTIRRVGRLGIDPQRHEVTAGGTPIDCTPGEFAILAAMAELPDRVFTRGQLLEHTSGLDRDSTERTIDVHVMNLRRKIEADPRRPAQLLTVYGIGYKLTAGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W881</accession>
    <name>U5W881_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45354">
        <property type="protein sequence ID" value="AGZ45354"/>
        <property type="gene ID" value="AFR_35490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7182"/>
    <dbReference type="eggNOG" id="COG2815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1092507_0_0_11"/>
    <dbReference type="OrthoDB" id="2191200at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="254" mass="26286" checksum="203EBF9252807279" modified="2014-01-22" version="1">MTAGATDRKNRWLRLPAGLVLLVVFPLIAAGWAVYTVALRILHRNTASAPSPARTAVAALIIIVLGTAGAGVYVLSARPSESVVAVQTQPVPAQPIPTPETTPAASVPPGAVMPDALGMPLPAAESLVRSIGLRYVSTDRSPLGRRLTIDTSWTVVASNPRAGRPVFAGGRVALYALKNNEAAWFAAHPVMPRLAAGATSASLVGPGDQLADVEELVSFTEGARPGTVPAAGRRLRPGQLIVVTTKPGNPTIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8U2</accession>
    <name>U5W8U2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative M16-family peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_35905</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35905"/>
    <dbReference type="EnsemblBacteria" id="AGZ45437">
        <property type="protein sequence ID" value="AGZ45437"/>
        <property type="gene ID" value="AFR_35905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7267"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665812" evidence="4">
        <location>
            <begin position="34"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16" evidence="5">
        <location>
            <begin position="79"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="6">
        <location>
            <begin position="235"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="490" mass="52244" checksum="79797E5540FFB1CC" modified="2014-01-22" version="1">MPPPLLRVRTTADARRARTVARVVRVVASPAAVASVPAATATDSRMTESSRGPAERPARVSTRTLQDGVKKTVLPSGLRIITEAIPTTRSAALGVWVAVGSRDETPAMSGASHFLEHLLFKGTEKRTALEISAEIEAVGGETNAFTTKEYTCYYARVLDADLPLAVDVLCDAVSRSILDPADVETERGVILEEIAMHDDEPGDEVHDVFTQAIFGADTPLGRLISGTEETISPMTRVTINRFYRSRYKAPQVVITAAGNLDHTRVVKLVRAALAGSPFDEGQASPAAKRASSGGPRPRKPTTLVRHRDTEQAHIVLGCAGIDRADERRFALGVLNNVLGGGMSSRLFQEIREKRGLAYSVYSYGSQYADAGLFAVYAGCAPGKAEEVLALIRAELAVVAAEGITPDELARGKGMVKGSYVLGLEDTGSRMSRLAKSELLHGDLLGVDELLAEVDAVTLDEVNVLAAELLSKPMSLAVVGPFDENAFSTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8V8</accession>
    <name>U5W8V8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36465"/>
    <dbReference type="EnsemblBacteria" id="AGZ45549">
        <property type="protein sequence ID" value="AGZ45549"/>
        <property type="gene ID" value="AFR_36465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36465"/>
    <dbReference type="HOGENOM" id="CLU_2115740_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="114" mass="11922" checksum="4FEAF7FDECE30A9D" modified="2014-01-22" version="1">MAVVPPDEGFVASDRDGVGEDVTGLDDDVTGLDDDADAVGEDADGETGEGDPRSDAAGGADPVWPPHAAWVRSPASTTKTADTRWKETRFRTDFSLLMGGVVPRQPSVGRVARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W928</accession>
    <name>U5W928_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase I</fullName>
            <ecNumber evidence="3">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_36305</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase S26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36305"/>
    <dbReference type="MEROPS" id="S26.025"/>
    <dbReference type="EnsemblBacteria" id="AGZ45517">
        <property type="protein sequence ID" value="AGZ45517"/>
        <property type="gene ID" value="AFR_36305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7347"/>
    <dbReference type="eggNOG" id="COG0681">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028723_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S26" evidence="4">
        <location>
            <begin position="11"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="31014" checksum="2E64C67DC5244A40" modified="2014-01-22" version="1">MIRRKEMPLWQELPLLLVVAFCLAVLIRTFLVQAFYIPSGSMENTLAIKDRVLVNKVVYDMRDPLRGEIVVFRGTDNWAPEQPLDPVSNTFGAKLGRTIGDLVGVSRPGERDFIKRVIGLPGDKVACCDDQGRITVNGEPIDEPYIFENSPLEAPPNPRQCTSRRFAEVTIPAGEMFVMGDHRMVSQDARCQGPVPIDNIIGRAFVVVWPTKHFTSLSVPDNWKAYGEAHPVAAAPAGVYPAREPDPGTVAVMLPFLLSVGVSARSGLWLRLRRRRLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W934</accession>
    <name>U5W934_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cellobiose ABC transporter solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561975.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36925"/>
    <dbReference type="EnsemblBacteria" id="AGZ45639">
        <property type="protein sequence ID" value="AGZ45639"/>
        <property type="gene ID" value="AFR_36925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7471"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_2_3_11"/>
    <dbReference type="OrthoDB" id="534010at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="427" mass="46175" checksum="402F0C32103C433D" modified="2014-01-22" version="1">MERFAKRGVALVAAGLAGTLAIAGCSGQSLEGGSDKAADGQITLKVNFWGDMGLDKLKAAYEKDHPNVKIVLNSGEYNAQHEDLQKKLVAGSGAPDISAVDEGFIVQFRGQSDKFVNLLDKGAASYEPKYLPWKWQQSMSADKKQIGLGTDVGGLAMCYRTDLFKAAGLPTDRESVSKLWPTWDDFIATGKKYVAATDKKFVDSGTNMFNPVLAQQPQGFYDESEALQMDVGPKVAFDVSMKAIEAGLSANLASFQPNWDQGFKKDQFATLACPAWMLGHIQDTAPEQKGKWDIAAIPGGGGNWGGSWWTIPSQGKNTDEAYKFVEWMVQPAQQIEVFKTVGNLPSQPALYKDAAVLDYKKEFMSNAPTGQIFAATAEGLKPQYLGKKNGPTRVAVENVITRVQQGSLKPEAAWPEAVKAAQKAATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAA0</accession>
    <name>U5WAA0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561208.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33065"/>
    <dbReference type="EnsemblBacteria" id="AGZ44871">
        <property type="protein sequence ID" value="AGZ44871"/>
        <property type="gene ID" value="AFR_33065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6692"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_19_1_11"/>
    <dbReference type="OrthoDB" id="1840839at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="136"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="36080" checksum="8F5E56723C28CEA7" modified="2014-01-22" version="1">MTATRIGLVGYGSGGRIFHAPLLASADGVEFAGVVTRSPERRAELAKAHPGVAAYDSLADLAAAGVQAVAISTPAATHVPLAREAVELGLAVVVDKPFALEAAAAREVTTLAGEAGVLLTVYQNRRWDSDLLTLQRLIGEGALGEIRRFESRFERWSPDREPPAAGGGTLLDFGAHLVDQAHLLFGPSTRVYAEMRGDGDRDDDVFVALHHESGVESHLWGSWRQAAPGPRFRVSGTTGTYIVDGIDGQEAALKAGRSPADLGERWGVEPEHAWGRLYRGATGAPVRSERGRWDSFYPAFAAAVRGEGPLPVDPWDSVRNMDVLDAARTSATTGRSVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAB5</accession>
    <name>U5WAB5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha amylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33140"/>
    <dbReference type="EnsemblBacteria" id="AGZ44886">
        <property type="protein sequence ID" value="AGZ44886"/>
        <property type="gene ID" value="AFR_33140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6706"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006462_2_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="1">
        <location>
            <begin position="32"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="548" mass="61841" checksum="3AB435CF8BEAC9BA" modified="2014-01-22" version="1">MRPKPHVETIRKQVIVAISDQDPWWRDAVIYQVYPRSFADSDGDGIGDIDGIRAHLDHLSDLGVDAIWMSPWYPSPMADAGYDVADFRDIDPAFGTLAGAEALVTEAHAQGIRMIVDIVPNHISSEHLWFKKALADPEAPERELFWFRPGRGPDGDQMPTDWVGEFGGTTWTRTTNPDGTPGDWYLHLFTPEQPDLNWDHPAVREEFEDILRFWFDRGVDGIRIDSAALLFKDRDLPAVVDGQRHPFHDLDEVHDVYRAWRRVSDSYPSRALIGEVWMPEVDRFANYLRPDELHAAFNFDFLGCAWDPQLMRECIDRTLHAHAAIGAPATWVLSNHDVTRHVTRYGRTETTFSFENNLDGTPVDLELGTRRARAAALLSLSLPGSTYVYQGEELGLWENENIPVDQMQDPMYARRGHTRDGCRVPLPWTGDEAPFGFTTGEPWLPQPAEWKDRTVEAQTGDPNSMLELYRSAIRARKTEAGLHQDGMTWLDSGPGVLAYTRGRDFACVLNMSGAAVALPDHHSVLLTSGPLDGTLLPQDTAVWLRIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAG5</accession>
    <name>U5WAG5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562516.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39645"/>
    <dbReference type="EnsemblBacteria" id="AGZ46183">
        <property type="protein sequence ID" value="AGZ46183"/>
        <property type="gene ID" value="AFR_39645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8020"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="11903" checksum="DF02D4E7F31E85D8" modified="2014-01-22" version="1">MATFKEHQGWEALGDPSRFAIVKCLAEGPRAVGEIADQLPISRQAVSQHLRVLKDAGLVADRAQGTRRVYRLNPAGVAALRDQLDTFWRRALDSYQSLAEDTTEEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAG8</accession>
    <name>U5WAG8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561278.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33415"/>
    <dbReference type="EnsemblBacteria" id="AGZ44941">
        <property type="protein sequence ID" value="AGZ44941"/>
        <property type="gene ID" value="AFR_33415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6763"/>
    <dbReference type="eggNOG" id="ENOG502Z7P1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025722_0_0_11"/>
    <dbReference type="OrthoDB" id="826678at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025566">
        <property type="entry name" value="DUF4331"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14224">
        <property type="entry name" value="DUF4331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665717" evidence="1">
        <location>
            <begin position="36"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="465" mass="49748" checksum="3641B78E2290799D" modified="2014-01-22" version="1">MRFTEFKRRRGLVIVTAAGVTAACVASLAPVQSIASSHREAPLIASTPSLDNTDLYAFVSPDDANTVTMVASWQPFEEPNGGPNFYPFAEKTAHDINIDNNGDAKPDITYRWMFTTSYQNPNTFLYNTGVVSSLQDKDLNVRQTYKLERITPSGTKTILSSARVAPSFTGKASMPNYESLSNAAVTSAGAGAKSFAGQSDDAFFADLRVFDLLYGGDLTEVGQDTLKGYNVNTIALKVPKSELALKGDATRNPVVGIWSTTSRMGASMHGKPATMNQVSRLGMPLVNEVVIPLGKKNEFNATKADSDAKFLSYVQKPEVPKLIKAIYNIPAPAEPRNDLVEVFLTGVCKACGPVAADLNSQKLNKDVNPKAFAPSEQLRLNMAVPTTATPNRLGVVGGDLAGFPNGRRLSDDVIDITLQAAEGILLPNPPAAVATLGDGVNTNDHAFRSSFPYVSLSNMTTVSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAN9</accession>
    <name>U5WAN9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-dependent synthetase and ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562386.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38990"/>
    <dbReference type="EnsemblBacteria" id="AGZ46052">
        <property type="protein sequence ID" value="AGZ46052"/>
        <property type="gene ID" value="AFR_38990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7891"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_11"/>
    <dbReference type="OrthoDB" id="961884at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="12"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="425"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="511" mass="54857" checksum="CFD425435C422E3C" modified="2014-01-22" version="1">MTMLSLAAVLAEGARRYPDKVAVVDGAARITYHELWEQSRSYAAGLRELGVGPGDTVAILIPNLADFPRAYYGALAVGAQLVPVHLLLTAEEMAYVLRDSRTDLLVAHSSQLKTAAAAAKLAGVKVASVGPMPPGIPDAPPRLEEAAAGVPPLPTYVSREAEDVAVIFYTSGTTGEPKGALLTHLNLVMNATVNVFDANEVNSSDVVMGCLPLFHTFGQTVGMNGTFRLGGTLVLLARFTGEAAIELMVREHVTVFHGVPTMYIGLLEAAAKADKLPELRICVSGGASLPVAVLERFSETFHSTVFEGYGLSETSPTATTNQPAFGTRAGTIGHPIWGVEVEIARAEIDDRIELLPAGELGEIVIRGHNVFAGYLNRPEETAQAVVDGWFRSGDLGTKDAEGFITIVDRKKDLVIRGGFNVYPREVEEVLARHPSVVQVAVIGVPDEVFGEEICAVVVADPAGITEDELIEWSKEQLGRHKYPRQVRFVDALPMGPSFKVLKRELRRTFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB36</accession>
    <name>U5WB36_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40870"/>
    <dbReference type="EnsemblBacteria" id="AGZ46428">
        <property type="protein sequence ID" value="AGZ46428"/>
        <property type="gene ID" value="AFR_40870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8274"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_13_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="408" mass="42500" checksum="883972483587ACA3" modified="2014-01-22" version="1">MFWRWWAAGTSSDLGSAVGGVALPLTALTVLDATAFEMGMIAAAGYVAWIVIGLPAGIIVQRLPLRGTQVAADLIRAAAVASIPVAWWLGVLTVTQLIVAALVVSFAHVIFFVANTTFLPEIVSRDQLQSRNSLTSGTHATTQLAGPSLGGLAVQLLGAVPTLLVDAVSYLVSAALLRTLPARHGHRPDRPTPMGAMIREGWAFVVKHPMMGPTMWAATAMNFVSGAYHALFALYLVRELHAPAGVVGVLLAMDGLGTLIGAALTTRVTNALGTARGLIILGFVEAAGAFLIPVGTGWAAYAAFTAGVLITACLSCIFSVTTRTYRQIASPPELLSRVMATVRFVSWGAIPVGGLVAGALAGPFGARATLFVFAFAALLGPLALFFSPVRGERDLVLPPVPEREASAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB63</accession>
    <name>U5WB63_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase molybdopterin-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561551.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34795"/>
    <dbReference type="EnsemblBacteria" id="AGZ45215">
        <property type="protein sequence ID" value="AGZ45215"/>
        <property type="gene ID" value="AFR_34795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7040"/>
    <dbReference type="eggNOG" id="COG1319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058050_1_0_11"/>
    <dbReference type="OrthoDB" id="1017413at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="SSF55447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="34409" checksum="A5F7CF7EBFA14ED9" modified="2014-01-22" version="1">MKTFDYAPATDVAAAVALASQGGRFLAGGTNLVDLMKLGVETPDVLVDIARLPLREVTELPGGGLRIGAGVRNSDLAAHPVVRRDFPVLSQALLAGASGQLRNMATTGGNLLQRTRCRYFMDMTKPCNKHAPGTGCPARNGDHRNLAILGASEHCVATHPSDMAVALVALDAIVQITGPDGDRSLPLTELHRLPGDTPHLDTVLTAGELITAVDVPALGFGGVSAYRKARDRASYAFAVGSVAAALHAEDGVVRDVRLALGAVAHKPWRAFVAEEALRGRPANRESFLAAADAELAAARPLRDNGYKRTLARNLVATTLERLTGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB71</accession>
    <name>U5WB71_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562570.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39915"/>
    <dbReference type="EnsemblBacteria" id="AGZ46237">
        <property type="protein sequence ID" value="AGZ46237"/>
        <property type="gene ID" value="AFR_39915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8076"/>
    <dbReference type="eggNOG" id="COG2331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136025_1_0_11"/>
    <dbReference type="OrthoDB" id="1914830at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013429">
        <property type="entry name" value="Regulatory_FmdB_Zinc_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09723">
        <property type="entry name" value="Zn-ribbon_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00834">
        <property type="entry name" value="CxxC_CXXC_SSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02605">
        <property type="entry name" value="CxxC_CxxC_SSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxxC_CXXC_SSSS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="12043" checksum="EC8D92800927B13D" modified="2014-01-22" version="1">MPTYQYACTECGEQLEAVQSFSDPALTECPNCSGKLRKVFNSVGIVFKGSGFYRNDSRSGNVGAEKAEKPAAAASSSSSDSKSSDSSSSTTPASTTPASSSSSSSSTSSASSSTSSSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBA5</accession>
    <name>U5WBA5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-like N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562761.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40875"/>
    <dbReference type="EnsemblBacteria" id="AGZ46429">
        <property type="protein sequence ID" value="AGZ46429"/>
        <property type="gene ID" value="AFR_40875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8275"/>
    <dbReference type="eggNOG" id="COG3153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074359_1_0_11"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="205"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="333" mass="34628" checksum="E0FDD71B982257B5" modified="2014-01-22" version="1">MDSEFWISELTDDDVPAVVALCRTALDLPEDAAEAAEIVLRLRETTGTAGWSATPRKLAGFIALRSAGAGPAAAGAALSGEPVSVTGGRVIGVVLGSVSHRDRSIGHVDLVAVDPRERRRGVARALVSRVEGALAGLGAGDIVIAGNAPYYAWPGIDVRYTPAICAAMAFGFEQDQPAWNMTVDTAVAGLESTADHEERLAGEGITVRRAGAEDVPALVSFALATFGGGWAGEITHSVGRDQAGCHLALAKDGSILGFAAYGSSRPSWFGPMGTATAARGLGIGALLLRRCLADQRAAGHETVQIGWVGPLPFYSKAVGARVERVFLLYRKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBE3</accession>
    <name>U5WBE3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562639.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40265"/>
    <dbReference type="EnsemblBacteria" id="AGZ46307">
        <property type="protein sequence ID" value="AGZ46307"/>
        <property type="gene ID" value="AFR_40265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8149"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_5_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="297" mass="31998" checksum="19E03E590435CC42" modified="2014-01-22" version="1">MAYIVTGPTAGIGRRTALELAKRDTVILVGRDPAKLDDVRKTIERDGGRAVPIVCDLSDLASVRRAAAEIIGLGLPLTGLLNNAGIMQMHPTTNTLGWDLTFATDHLGPFALTEALTPHLTDGAQVVFICSSCEDPERRPAVIAGFRGGRYLSAEASARGDWAPGGSKLPGRDAYATAKQCNLATVLVFAREQPRLRFNAVEPGFNPATDLGRDSNVFLRAVAKYVLSPLAPFIKYWSNPRTAARVITRVLTDTSGGTGVYYDENGRPFQGSELVHQPEFSERVVAETRALLARVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBH0</accession>
    <name>U5WBH0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41200"/>
    <dbReference type="EnsemblBacteria" id="AGZ46494">
        <property type="protein sequence ID" value="AGZ46494"/>
        <property type="gene ID" value="AFR_41200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8338"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_718929_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="367" mass="38031" checksum="2D20F29992FBA978" modified="2014-01-22" version="1">MTLPRALAALLVLAAAGLIGWRVLAPAEVIEEAVEPYPVAVTLPAGVTGKTTMAPLIVDGRIRVYAGTRLVKADAPVEAKTMYTPLWSYRRWPARLAGVVAVGTTVVSRWSDGRLVALDGRTGKVVWRADGPPADGFAGRTGSAAVWAPAGMHVAGTSVLVSDGKQVTARAVEDGRERWSTTLPPGCTDGFVTAGGRYVCGAGAWDVISGNEVRGWPAGPSTAMSCDVARSACAGLRDASGQVWLTGGRRPVPAPGSAVAGDLALTVSGGAVIASRAGREVWRWAGTAQVLGVRAGKVVLLAGNQLVVLDAATGAARATFPLFVANERVEAWQPDGWQLTDGYVAIQRVRPGTSEFYTTDPVVIAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBK4</accession>
    <name>U5WBK4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydropteroate synthase</fullName>
            <shortName evidence="1">DHPS</shortName>
            <ecNumber evidence="1">2.5.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dihydropteroate pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DHPS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41470"/>
    <dbReference type="EnsemblBacteria" id="AGZ46548">
        <property type="protein sequence ID" value="AGZ46548"/>
        <property type="gene ID" value="AFR_41470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8391"/>
    <dbReference type="eggNOG" id="COG0294">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008023_0_1_11"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00156"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="PTHR20941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="2">
        <location>
            <begin position="24"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="31107" checksum="C09A76E34F0854A4" modified="2014-01-22" version="1">MSDPIGSATTAQVSGDLLLQQDHPVVMGVLNVTPDSFSDGGRFAGVEAAVAHGLALRRAGADIIDIGGESTRPGADRVDGPTEIARVLPVVTELARAGVPLSIDTTRAEVAAAALAAGAVVVNDVSGGLADPGMATVVADAGCPWILMHWRGHSRRMLDLATYTDVVAEVRAELLQRVEDAVSAGVDPGRLILDPGIGFAKRAEHNWALSAHLDQLVALGHPVLFAASRKTYLGRLLASPDGEPRPVDGREAATIATSVLAVAAGAWGVRVHDVRATADALAVWRATGRPRFQRSSSGGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBS9</accession>
    <name>U5WBS9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative competence-damage inducible protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561752.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35800"/>
    <dbReference type="EnsemblBacteria" id="AGZ45416">
        <property type="protein sequence ID" value="AGZ45416"/>
        <property type="gene ID" value="AFR_35800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7246"/>
    <dbReference type="eggNOG" id="COG1546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_1_0_11"/>
    <dbReference type="OrthoDB" id="1834406at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="SSF142433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00199">
        <property type="entry name" value="PncC_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="15543" checksum="D2C6573C1D10ECE9" modified="2014-01-22" version="1">MIAAEATRALIERRQTLAVAESLTGGLLAATIVEVPGVSATFRGGLVVYATDLKHSLAGVDEALLADRGAVDPDVALALAEGARSRCGADWGLATTGVAGPDPQDGKPVGLVYIAAAGPGRTAVRELRLSGSRDAIRTATVSAVLELLATELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC30</accession>
    <name>U5WC30_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42200"/>
    <dbReference type="EnsemblBacteria" id="AGZ46694">
        <property type="protein sequence ID" value="AGZ46694"/>
        <property type="gene ID" value="AFR_42200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8541"/>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016537_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="542" mass="58659" checksum="52A1FA957535DA55" modified="2014-01-22" version="1">MLAHGARVLTDGAYPFFSDRMNYPDGVNMMANTSVLAVSLPMTPITVLFGPHVSFNAFLTLALAVTGISWYLVLSRKFVSSRLAAWVGALFATFAPSMIAHAGGHPNIVSQFLVPLIIWRTLALRTPGRAIRGGLILAGLLVWQAFINLEILFMTAVGLGIFCAVMAISRRKAKPAEIKTFLRGLGIAAAVTLAVLAYPLCVQFFGPQSYHGLPEFVRYFGADLGSFTAYAQRSVAGNSITAGRLAQNAAEENAFFGWGLVILFFGLVVWMRRSVAVVTLAGVALFFGAMSLGPSIRLNGVDTGVPGLWALLHSVPVLNSAVPTRWAMAIAPIVGILLALGCQRASDLMKAQPAARGPVRVAMITAVAMALVPLVPTPLRIAPMDPVPDFVTTGAWKQYVDDNHTLVALPLPDSSYPDPMRWSAYTGQDMRIASGYALLPNQNPLDPTDRSALFAPPWRPTSGLMSSIKQGNPIPEITETRREMTLADLRYWKAGVVVLTPQVRDIEMLRAMTELLGFQPTWTGGAWIWDVRNLVDAEKVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC45</accession>
    <name>U5WC45_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36445"/>
    <dbReference type="EnsemblBacteria" id="AGZ45545">
        <property type="protein sequence ID" value="AGZ45545"/>
        <property type="gene ID" value="AFR_36445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7375"/>
    <dbReference type="eggNOG" id="COG2318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097062_2_0_11"/>
    <dbReference type="OrthoDB" id="2047691at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007061">
        <property type="entry name" value="DUF664"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04978">
        <property type="entry name" value="DUF664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="19589" checksum="F2D504CA5BE54C2D" modified="2014-01-22" version="1">MTSWTAPAAQRVDEPFTGDERTMLEGWLDWHRQTLLSKCEGLTAEQLKLQAVEPSGLSLLGLVRHMSEVERGWFRRNAAGEDVPQIYCSDTDEDGDFDNIDTADAEADFATFHAEIEACRAAMTGVPLEHEFATPRRPVISVRWVHLHMIEEYARHNGHADLLRERIDGRTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC72</accession>
    <name>U5WC72_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561872.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36400"/>
    <dbReference type="EnsemblBacteria" id="AGZ45536">
        <property type="protein sequence ID" value="AGZ45536"/>
        <property type="gene ID" value="AFR_36400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7366"/>
    <dbReference type="eggNOG" id="COG4122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_796541_0_0_11"/>
    <dbReference type="OrthoDB" id="1900999at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="345" mass="37832" checksum="8C54DB9C0793CA5C" modified="2014-01-22" version="1">MSSLVRFGGRLAVRLRSLTRPQAVVLMAILALCLGVWAAAATGHQGLAVSLVAVLLLGVLLGILHLSRWIGGVYRANQTANREMRTVVEQLQRRVVATVEKERLSAGDRHQDLTRTITRGLRQSGHGTDLLLRAQSREIEAMFQLFQQFTPRAPMPSSGDFALNPTDLLDLLHLVRLREPRLVVELGSGTSSVWLGYALEQYGGKLISLDHDADYARKTRAMLAAHGLNAVVEVREAPLRELTATGKTYQWYDTQALDDLTGVDLLLVDGPPAATGPDARYPALSVLEKRLASTATVVLDDANRQDERDAVLRWTQEVPGLTEEQRLLGRHAVLSYSRTGAPVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCK1</accession>
    <name>U5WCK1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sensor histidine kinase and response regulator of a two component complex</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563028.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42215"/>
    <dbReference type="EnsemblBacteria" id="AGZ46697">
        <property type="protein sequence ID" value="AGZ46697"/>
        <property type="gene ID" value="AFR_42215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8545"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_44_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="186"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="400" mass="43958" checksum="B85BC244A8C55B77" modified="2014-01-22" version="1">MRAPLPTDENDRVAALHDTRVLDTLPEDDFDDIALLASEICGTPMGLVSLVDTDRQWFKAKVGLEVDEMHRDMSFCAHAVTGHDLFEVPDALADARFADNPLVLGDDNVRFYAGAPVVLDGTHSVGTVCVVDHVPRELSTGQRRALRSLARHASVQLELRRYARHAGDIADRMRQLDRMKDSFLAGVSHELRTPLASIRGYLEMLLEDDFDPDTSQRFLSVMQRNSDRLLRLIDDLLSVARLNGEGVDLDVVELDLAELAHQVTTSCRPLAEHREITLLNSTERPILARGDARRLGQALNHLIVNAIKFTSPGGEITICGTEGDEPEIIIRDTGVGIPAAELPHLFDRFFRSAAADQMAVQGPGLGLSIVRLIIDAHHGSIHVDSEPGIGTAIRLTLPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCM0</accession>
    <name>U5WCM0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Threonine synthase</fullName>
            <ecNumber evidence="6 10">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AFR_37480</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 10">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>H2O + O-phospho-L-homoserine = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="6 10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562085.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37480"/>
    <dbReference type="EnsemblBacteria" id="AGZ45750">
        <property type="protein sequence ID" value="AGZ45750"/>
        <property type="gene ID" value="AFR_37480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7581"/>
    <dbReference type="eggNOG" id="COG0498">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_0_0_11"/>
    <dbReference type="OrthoDB" id="428821at2"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="14">
        <location>
            <begin position="22"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="11">
        <location>
            <begin position="185"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="cross-link" description="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)" evidence="13">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ45750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="349" mass="36156" checksum="9FFA7BFC677F4927" modified="2014-01-22" version="1">MWRGLIEAYRDRLPVTEATPVVTLHEGNTPLVPAPVLSQRTGADVYLKVEGANPTGSFKDRGMTMAVSKAVEEGSKAIICASTGNTSASAAAYAARAGITCAVLVPQGKIALGKLAQALVHGAKLLQVNGNFDDCLALASKLSQDFPVSLVNSVNIFRLHGQKTAAFEIVEALGTAPDIHCLPVGNAGNISAYWMGYQEDHEAGNSKILPKMYGFQASGAAPIVNGQVVPEPSTIATAIRIGNPASWTKALDARDNSGGLISAVTDREILSAYRLLAREVGVFVELGSAASVAGLLQQAEAGMIPAGSTVVCTVTGHGLKDPEWAISTAPSPTTIQNDVLAAARALDMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD63</accession>
    <name>U5WD63_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycoside hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_38185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38185"/>
    <dbReference type="EnsemblBacteria" id="AGZ45891">
        <property type="protein sequence ID" value="AGZ45891"/>
        <property type="gene ID" value="AFR_38185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7725"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_846310_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003610">
        <property type="entry name" value="CBM_fam5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036573">
        <property type="entry name" value="CBM_sf_5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="GH16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00495">
        <property type="entry name" value="ChtBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51055">
        <property type="entry name" value="SSF51055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH16" evidence="3">
        <location>
            <begin position="23"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="235"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="334" mass="35661" checksum="92CC3118C31E32B0" modified="2014-01-22" version="1">MAAAGLATAIWIPARNDSADASERNRNGTNDRFTRDRGEARGAFSDDFSGRRGSTVDPVKWVLNTDRADNGLEFRESTRNARLDGDGNLTIVLRREDRGEFSSARLVTRDTLRGASGHVEARIKTPEGQGVRPAFELIGSGRQSFGSFDVLTEPLSDDFHTYAVDWAPGEVVLSVDGQEIDRLTPENLDTDQPFRLALSLAVGADRSGTGFPARMQVDSVSFAAAGDEAPPTTSPTAEPTTPPAEPTTPPTTAPTSEPPTAEPTTPPATTPPAETTAPTTPPTTAPAAKAWAPFTDYVAGQLVTFKGVEYQVQETHTSLPGWEPTALPSLFKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD83</accession>
    <name>U5WD83_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38285"/>
    <dbReference type="EnsemblBacteria" id="AGZ45911">
        <property type="protein sequence ID" value="AGZ45911"/>
        <property type="gene ID" value="AFR_38285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7745"/>
    <dbReference type="eggNOG" id="COG4899">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929484_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018697">
        <property type="entry name" value="DUF2199"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09965">
        <property type="entry name" value="DUF2199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="31875" checksum="9E3308B667C5DD12" modified="2014-01-22" version="1">MSPVDLAVRSAWPDPLLALSERKREATWGNEHLRHANGIGSFVRCLMPVRLSGGGSIEYSVWLQVSKDELKHAHKIWEKPEYAGLRLEGTVSNSIKPWDGLLGTPGRAEVRDPDSIPYLVADEGTLLHRILHDEWDRDDVLSRLTYALPTPVHQRITDDWSLERTAGLELKWHEDRIYFSGPGRTVHLDPLGAPPGYTPQRMIAELTEDAPRDRDGEATEDDGEIHRHAFWRPALGDGRIVHNLHGFVAVEGKLLHVACVFDDPSDLPWAQRVWRSVRPEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDC6</accession>
    <name>U5WDC6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_38485</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562285.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38485"/>
    <dbReference type="EnsemblBacteria" id="AGZ45951">
        <property type="protein sequence ID" value="AGZ45951"/>
        <property type="gene ID" value="AFR_38485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7786"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_0_11"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="36628" checksum="5A001FFC8EEBEE42" modified="2014-01-22" version="1">MPPQRATLLQVAQRAGVSRSTASFVLAGRHVDMRISEDARQRVLRAAQELDYRPNLMARSLRTKVTKTIALVSDTLASDPYAGRAIHGGLAAAVAHGHLLFIGETEGDAVVEEKLISDFLDRQVDAFVYASMFTRYVRVPKLLKGHPVVLLNCLTRAARPAYHSVIPDELAAGWSAAKVVIDGGHRDGIHLVGNPAQHVFAGRERLAGIREAFTAAGIKLAGTIECDWWPDSAYEAVTRALAGGPPPKALICFNDRVALGVYQALREAGLSIPGDVSVVSFDDSDLAAWLRPQLTSISLPHYQLGWQAVELLLGEAGRSEPAIRRIPMPVRSRGSTGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE36</accession>
    <name>U5WE36_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycoside hydrolase family 43 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_39660</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562519.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39660"/>
    <dbReference type="EnsemblBacteria" id="AGZ46186">
        <property type="protein sequence ID" value="AGZ46186"/>
        <property type="gene ID" value="AFR_39660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8024"/>
    <dbReference type="eggNOG" id="COG3507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_388142_0_0_11"/>
    <dbReference type="OrthoDB" id="442641at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665808" evidence="6">
        <location>
            <begin position="23"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="5">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="711" mass="76647" checksum="957F5507904CA629" modified="2014-01-22" version="1">MRRFTAALAAVLLLLTPAPARANPVSASFADTFADPMVLRGDDGYWYAYGTTDPLREGEKVAHRIPTARSSDLTSWTYLGDAFTAGQRPAFAAENAVFWAPDVRRSGDHWIMYVTVTETTVAPGASAIGAATAPTPAGPWTFADEPVVAPRPSGDGGWLWTFDPAQLTTPDGRQYLYYGSYFGGIWVSELSADGLRITGTPTRVAIDNKFEGAYVVRRDGWYYLFASSANCCAGPTTGYSVSAGRSRSPRGPFTDRDGLRLDVSRAGGTPVIAPNGNRWVGTGHNGLITDLAGQDWLAYHAIDRADPYLDEPFGINERPMLLDRLDWIDGWPTVRAGAWASDRLVTEPVTRTPLQDRFDTLSARTWQVVDGRWAVADGRLRGQGTLVARKPLPGDLRVEADLRGPAGLTIDTLSVRAEHSTLVIRSGRRTVAQTHIAPSTSLRHLSVERRGGQVTAMLDSVRLDARVHGRGPGIVGLTATAEAEWDNLSAGRPADPVRRAVAVPQPGRALNAYSDDFRGTALGAGWSFVRPDAAATVSGGALRWPVQNADLTGDSNTAGVLLRDAPAGRWIAETKVTLDLGEDTVRNYQQAGIVAYAGDDEFARLSAVAIWNTRQVEFGHEIPYAGATSYGGTIVGAPGLTTTWLRLAHSIDPRNGEHEYRAGVSRDGRTWTWGGVWTLPADSAPRIGLVAHGGAEPPVTAAFDYLRFSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEC9</accession>
    <name>U5WEC9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dsre family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40705"/>
    <dbReference type="EnsemblBacteria" id="AGZ46395">
        <property type="protein sequence ID" value="AGZ46395"/>
        <property type="gene ID" value="AFR_40705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8238"/>
    <dbReference type="eggNOG" id="COG2044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139144_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="12517" checksum="1204D728FB83904A" modified="2014-01-22" version="1">MARLLVVKATAGADAPERCSQAFTVASTAAASGVDVSFWLTGESTWFALPDRAAQFELPHAAPLPDLLELLLTSGRVTACTQCAARRNIGPDDVLPGIRIAGAAVFVEEIMSDGAQAVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEW6</accession>
    <name>U5WEW6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41455"/>
    <dbReference type="EnsemblBacteria" id="AGZ46545">
        <property type="protein sequence ID" value="AGZ46545"/>
        <property type="gene ID" value="AFR_41455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8388"/>
    <dbReference type="eggNOG" id="ENOG5033CMQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123281_2_1_11"/>
    <dbReference type="OrthoDB" id="2002261at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021517">
        <property type="entry name" value="DUF3180"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11377">
        <property type="entry name" value="DUF3180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="172" mass="17503" checksum="08F5125548425BAC" modified="2014-01-22" version="1">MSSNPGGGAPDPSMRPTSLSMLVVAGLAAAAVGWLLLSAFYTQMPRLPWLPIIVLATLAIAEAVLAQNTSARIQRKPGAPRVDPLAVARFVVLAKASSLAGALFGGFSAGLLAWLALEPTRAARDDVPAAVGGVAAALALVGAALWLERSCRVPEQPDQKGGGESGRQAGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEZ9</accession>
    <name>U5WEZ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative adenylate/guanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_41630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylyl cyclase class-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41630"/>
    <dbReference type="EnsemblBacteria" id="AGZ46580">
        <property type="protein sequence ID" value="AGZ46580"/>
        <property type="gene ID" value="AFR_41630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8425"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3899">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004435_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="35"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="784"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1182"/>
            <end position="1210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1210" mass="127209" checksum="9A3CE02FADC2FECE" modified="2014-01-22" version="1">MCGTVAVPGARFCHNCGAALPAAATLPAAERRIVTVLFGDLSDFTSWSEDLDPERVGAVTDRVLAALAGAVKTFGGHVDKLTGDGIMAVFGAPVAHEDDAERAVRAALSMQRAVRRVLDDERGGGAPLGLRVGLNTGEVVAGIQASIEYTVIGDTVNTAARLADAAAVGAVYAGSRTSAGTRHVASWRQLRALRLKGKREPVPAYELLGLHDAPGTRSGLGDEAPFVGRETELGRVSGRLAEVIDSGTPRVMVMTAEAGIGKTRFAGEVKRFAAGYDVGAGRFATHTGARVLRARCRAFGERRRFAPLADLVRKSVGLPKDVAATITRPVVEERLRKLVTRLTRDGEAPEVDIDRLLALLGYGDAPANPVGPAAVAEYQPTGGTRAETETIPVAVAGLLSALARETPLVVIVDDLHDATAETIDALGAVLSRLDGPVVLLLLGRPELVRTAGALTRLADAEIHTLPPLRGADASRLLTSYLNGGKLPQADADRLLATAQGNPFYLAELVTLLMERGALMPAVGANAAGKWQLAAGSMGSQLLSRDLAAVLAARIDALPTEPRSVLRDAAVVGDTVPTGVIEALRERRAPSDARPGAVAAVELERAVDELLQRRMLHRVRGGYAFSTPLMREAAYAGLGKADLADRHAYLARWAAPETVAALGYDSASRLSLGENERDAFVATHAECAVELADAVRLRPDATAREVTPLGVSALGRMARRALANIEPAASIQYAERAAGIAKDSLPLSDQLVHARALLRLGRASEALTYGEKIAESANEEPVCRAEALLVVGRAQEALGDSQRAVAAWQQALEVATEAELAPERANAMRRLGMADFLSGKLSQASSRFAAAYQVTLSAGDRHGQAWALQNLAWVTTTRGDFAGTDAVLGRAARLFAELGDPVGRSWLRGTTAFARLLAGRLHESRRLARIFLPFGDRVGEGWAVGTLRAVEAYAAAELGDLAEADGEARRAYREFAAVSDDWGRGLALVVRGAIARGLNEPEHAEDLLTDALGYADRTGHPLLLGMAGTLRGFVALQRGDVEAAEADARRVMTAVEPHNPLAPAQVGPRVLLAEARLRAGDAATAVGLLAPIASDTGGSLIFSRRHALASYASALLADGRVDTAAAWVRRAREVPAEDVRSGVVTAMVLARVNAAAGAMDEARDAAEEAVLLAYSTEQASERLSAEELRDALTHSTVEPPESVAYASDVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPK5</accession>
    <name>U5VPK5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_03045</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357841.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03045"/>
    <dbReference type="EnsemblBacteria" id="AGZ38898">
        <property type="protein sequence ID" value="AGZ38898"/>
        <property type="gene ID" value="AFR_03045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.613"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_2_0_11"/>
    <dbReference type="OrthoDB" id="1891092at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="9">
        <location>
            <begin position="33"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ38898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="29803" checksum="B0AE4826E25C1432" modified="2014-01-22" version="1">MTTMTRVPRTGSPILPTKVTPNPRPFRLVRHSLALAKRSLIKTARTPEALIDVTLQPVLFLVIFVYIFGGAVAGSTHDYLQFLLPGILAQTIATGCIAIGVNLNTDLAKGIFDRFRSLPVPRSAPLVGAVLGDVVRYVIVTVVTLAIGYVMGFRITTDPLSAIAGCALAVLFALCLSWLPVLVSMKVRTPGAVQGIMFALIMPLSFASNVFVGTSTLPGWMQSFVNVNPLTHLVGAMRGLFLGEPLGTHVWWTLAWCAGFVVVFMPLALRAYRKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPL0</accession>
    <name>U5VPL0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357846.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03070"/>
    <dbReference type="EnsemblBacteria" id="AGZ38903">
        <property type="protein sequence ID" value="AGZ38903"/>
        <property type="gene ID" value="AFR_03070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.618"/>
    <dbReference type="HOGENOM" id="CLU_1514773_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="177" mass="18586" checksum="6B66FE33E12E3DD8" modified="2014-01-22" version="1">MTSPAQSLDRRRDKASSAGAAADKAQAAVTDIDHQLDTIAGLTQQQKQALRHARDEAERLKRSLAAAAKRQSQLTRQRKKAVAQADKARGKAKAAEAKYDKEVLAELIRREKAASAGPAPVPADSAPDGSAREVAAKVTAAKASGARPPRRPAADPAPEKPSAATTTARRTAARATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ05</accession>
    <name>U5VQ05_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357978.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03720"/>
    <dbReference type="EnsemblBacteria" id="AGZ39033">
        <property type="protein sequence ID" value="AGZ39033"/>
        <property type="gene ID" value="AFR_03720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.751"/>
    <dbReference type="eggNOG" id="ENOG503375I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1293704_0_0_11"/>
    <dbReference type="OrthoDB" id="2061619at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="23798" checksum="F9790178EB052BD4" modified="2014-01-22" version="1">MEYLDLSPYEYRSFPLPLRNVGWLGTEWGVQGVDLPPLAAADLQLLRSASRLLGSVTLGTHRCEFCPEDAAVTGNGEYRYYLLNGDVYCAPEMVLHYLGDHGYRPPDVFLQGLRETGELEWDDRAERLRKVLLDPEADLGFRCAAVVDLPNWRDARALDAVQFAAHDEELAVIMGVEIGQSLVACLGDDLRAEDYPSTIGYGIDHARRLRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ37</accession>
    <name>U5VQ37_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative sodium/proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_03175</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357867.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03175"/>
    <dbReference type="EnsemblBacteria" id="AGZ38924">
        <property type="protein sequence ID" value="AGZ38924"/>
        <property type="gene ID" value="AFR_03175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.639"/>
    <dbReference type="eggNOG" id="COG0025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_8_2_11"/>
    <dbReference type="OrthoDB" id="1194802at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="PTHR10110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0915">Sodium</keyword>
    <keyword evidence="5" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="7">
        <location>
            <begin position="12"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="520" mass="55368" checksum="12920D1491B0C62A" modified="2014-01-22" version="1">MNGVQILLIIGAGIGISALAHRRGLQPGLIVVTLAAGVSFIPGVPRLELESELMLAIVVPPLLYSATRGASFTAFGRNFRAIFNLGVLLVVLTAGALGALSTWLLPTIGLAAAFVLGSVLAPPDTITTVTHGDELGLPKRVTTILTGESLVNDATALTLFSIAVAAVSGEHTTWGGGILEFFRNASIGAGIGGLLAIATLTLRKMLRNPTLETSLGLLVPFTAFLLAEQVHASGIIAVVVAAFSISINTSLDPRHQYPNAYRTRLQEDAFWPVVDFLLETFVFAYIGLQLKFVLEDLAAEEDPGLTRTLVAAGVLLLAAIVLRLTGVYALFGWWQLGDRNTKAKLARSPEFAERLNERRTRGRRNRRPREMPLDAPNARETMVVGWTGMRGILTLAAAASVPEMLANGEEFPGRSAIQAIALIVTLGTLLIQGTTIRRLVGLLHLDTSGERREAEEMRTRGVEVMTAAAPARDGGPTTDDYEKQRTAISTAVSDGTLDEETGRGLISDIDLRQAAAQASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQU2</accession>
    <name>U5VQU2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5 7">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplO</name>
        <name evidence="10" type="ORF">AFR_04600</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358203.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04600"/>
    <dbReference type="EnsemblBacteria" id="AGZ39209">
        <property type="protein sequence ID" value="AGZ39209"/>
        <property type="gene ID" value="AFR_04600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.929"/>
    <dbReference type="eggNOG" id="COG0200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055188_4_1_11"/>
    <dbReference type="OrthoDB" id="1874945at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_L15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="PTHR12934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L18e/L15P" evidence="9">
        <location>
            <begin position="28"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="147" mass="15432" checksum="00E2DF613FE9BA03" modified="2014-01-22" version="1">MAIKVHHLRPAPGAKTAKTRVGRGEGSKGKTAGRGTKGTKARYQVPARFEGGQMPLHMRLPKMKGFKNKNKVVFQVVNLERLAELFPNGGEVGPLELADAGAVRRGHPVKVLGSGELGSVTLQVSAHAFSESAKEKIAAAGGSATEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRI5</accession>
    <name>U5VRI5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphomannomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_05715</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358963.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05715"/>
    <dbReference type="EnsemblBacteria" id="AGZ39432">
        <property type="protein sequence ID" value="AGZ39432"/>
        <property type="gene ID" value="AFR_05715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1159"/>
    <dbReference type="eggNOG" id="COG1109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_9_2_11"/>
    <dbReference type="OrthoDB" id="256504at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular transferase activity, phosphotransferases"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="6">
        <location>
            <begin position="6"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="7">
        <location>
            <begin position="160"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="8">
        <location>
            <begin position="271"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="5">
        <location>
            <begin position="383"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="459" mass="48605" checksum="AF06C7F9FA6A91A0" modified="2014-01-22" version="1">MTDLSQLVKAYDVRGVVPDQLNETVARALGTAFVEMLRESGDAAERIVIAHDMRETGPGLAAAFAAGANSAGATVVHTGLGSTDQLYYASGVLGLPGAMFTASHNPAQYNGIKLCRAGARPVGQDSGLVVMRRYAEGLLDDLAAVGEVPPAIEHVNLLADYARHLRSLVDLSGIRPLKIVVDAGNGMGGYTVPAVLGDQELEALPLEIVPLYFELDGSFPNHEANPLDPANLVDLQAAVREHGADLGLAFDGDADRCFVVDENGDPVSPSAITALVAKRELAKFPGSTVIYNLITSHAVPEIIEENGGLPVRSRVGHSFIKAEMARTNAVFGGEHSAHYYFRDFWFADTGMLAALHVLAALGGQDQPLSLFAAEFERYSASGEINSTVADAAAKVAEVRAAYSGATFDELDGMTVQLADGAWFNLRASNTEPLLRLNVEAAKPDRMAALRDEVLAIVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSH4</accession>
    <name>U5VSH4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyridoxal-5'-phosphate-dependent protein subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_01435</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357519.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01435"/>
    <dbReference type="EnsemblBacteria" id="AGZ38576">
        <property type="protein sequence ID" value="AGZ38576"/>
        <property type="gene ID" value="AFR_01435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.289"/>
    <dbReference type="eggNOG" id="COG0031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046285_0_0_11"/>
    <dbReference type="OrthoDB" id="1677960at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="40"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="374" mass="39977" checksum="BAA60A67C66F1A05" modified="2014-01-22" version="1">MHQLDRCDDAARDWERNAIALVEADANRSADTHLLPFPLPRSWGIDLYLKDESSHPTGSLKHRLARSLFLYALCNGWIGPDTPIVEASSGSTAVSEAYFARMLGLEFIAVMPASTSPEKISLIEFQGGKCHLVDDPTAAVGAARTLAADLGGHFMDQFTHAERATDWRGNNNIAESIYAQLGLERHPVPAWIVVGAGTGGTSATIGRFARYRRFPTRLCVVDPEGSAFWPAYVASDWSVSTGRGSRIEGIGRPKVEASFQPSVVDRMVHVPDAASLAAMRSGSAVLGRGVGGSTGTNLWGAFGLIAEMLAAGRTGSVVTLICDGGERYADTYYSDDWVAAQGLDLEPHQATISTFLATGQWGQRGDGGPGRIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSP6</accession>
    <name>U5VSP6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isoprenyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359420.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08000"/>
    <dbReference type="EnsemblBacteria" id="AGZ39889">
        <property type="protein sequence ID" value="AGZ39889"/>
        <property type="gene ID" value="AFR_08000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1633"/>
    <dbReference type="eggNOG" id="COG0020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_1_2_11"/>
    <dbReference type="OrthoDB" id="1630604at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="205"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="29365" checksum="2E89260EFE6C3DD0" modified="2014-01-22" version="1">MTRPPIPHISGARPPALPAAAIPKHVAVVMDGNGRWAKERGLSRTKGHEQGEHSLFDTIEGAIELGIPYLSAYAFSTENWKRSPEEVRFLMGFNRDVIRRRRDQLVDLGVRVVWSGRAGRLWKSVISELQTAEEMSKDNKTLTLQFCVNYGGQAEIADATAAIARDVVAGKLKADKITEKTIQKYLYHPEIPEVDMFLRPSGEERTSNFLLWQSAYAELVFLDTLWPDFDRRHLWYACELYAQRDRRFGGAIPNPVAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSQ9</accession>
    <name>U5VSQ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein kinase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359378.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07790"/>
    <dbReference type="EnsemblBacteria" id="AGZ39847">
        <property type="protein sequence ID" value="AGZ39847"/>
        <property type="gene ID" value="AFR_07790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1590"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077925_0_0_11"/>
    <dbReference type="OrthoDB" id="1457558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="57"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="322" mass="33743" checksum="70765AFBE93801ED" modified="2014-01-22" version="1">MRVVSLPDVAYGATAVRPDWTDLPAAVRDAVSSRLGGPVTASASAGGGFTRAFAALVTTADGARAFVKAAPLSEPISDWYAREAAITAALPSEVPAARPRWTLQAGGYYVLCLEAVDGHVPNLPWTPADLAAALEAWRVSAEALARPSAELLGLGLPPLGAIVRDELSWWSEIAAGRAPLPPVPDWVPGRLGELAALEQGLPDLVAGPGMLHGDLRVDNVLLDAYGRARLCDWTWPCLGAQWFDTVTLLVTAYASGLDTDAALAPWDPPPDGVDGALAALGGYWLTRAAGGPSSASPHSRQHQRFSGNQALAWLAERRGWAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT14</accession>
    <name>U5VT14_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357734.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02510"/>
    <dbReference type="EnsemblBacteria" id="AGZ38791">
        <property type="protein sequence ID" value="AGZ38791"/>
        <property type="gene ID" value="AFR_02510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.504"/>
    <dbReference type="eggNOG" id="COG4842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_815427_0_0_11"/>
    <dbReference type="OrthoDB" id="1469466at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="375" mass="40987" checksum="F4A39560DC2CF4EE" modified="2014-01-22" version="1">MSQPSGQIWVNPEGVTQLGDNYGEHGALYQGYLDQLQLLRAQYGNSWGDDDMGKQFSEKFLKGLDALDGMVTGVKETLDYTSAGLRESGKAYRLADDDARDTGHKLARDFEGMPQGAGLNAYTAGEAQEAQPAQKLLGRTRRVAMSAGEVREAEPAQRLRGRLAVRAEPVEPVLRSEKNLQTGQDEQFVDVGDQSFRLKEIVWASNDEFVRKDGNTRLVSEGVDPETGEPKYTRISMDGKAYELEPQLRAMMSVMPAQEAQAHQAFPAISSYRMTPEYATALVDGRPLAEGYHLQALNRFPDGTTRVDANLFESIAPVVGSTVTGADGQPLDPGDDQFFVVKENPAADATAENYQPLIVSFTPDGTAVPLVTDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTB7</accession>
    <name>U5VTB7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08950"/>
    <dbReference type="EnsemblBacteria" id="AGZ40079">
        <property type="protein sequence ID" value="AGZ40079"/>
        <property type="gene ID" value="AFR_08950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1821"/>
    <dbReference type="eggNOG" id="COG4278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087628_0_0_11"/>
    <dbReference type="OrthoDB" id="1487865at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="181" mass="19351" checksum="86A93D937CA1AF2A" modified="2014-01-22" version="1">MRQRFASEHPALTADTIKTVEAGTRQWFRLAARHPKARLAMPSVVVDDLWHELVLHTREYAEFCDAAFGRFLHHTPEASTSGGLKETFRLAKQDEEGAVLPLIFRVDHDLGVPGGRHYLADCGGRGLCYELKGTVCIEHLAGPGKRPGGGWDFKRNAPDGGMSQAGGDPGGGCGGGCGGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUE8</accession>
    <name>U5VUE8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11050"/>
    <dbReference type="EnsemblBacteria" id="AGZ40499">
        <property type="protein sequence ID" value="AGZ40499"/>
        <property type="gene ID" value="AFR_11050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2251"/>
    <dbReference type="eggNOG" id="COG1361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000300_0_0_11"/>
    <dbReference type="OrthoDB" id="104673at1760"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665224" evidence="3">
        <location>
            <begin position="26"/>
            <end position="2433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2405"/>
            <end position="2426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1705"/>
            <end position="1724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="2433" mass="240179" checksum="9EE13466E6AFB449" modified="2014-01-22" version="1">MRSAALLLAVLVAVGLVGPPTAAQAAAGTINTTAQGVRAAITLLGGISLPITTPQSTWQTGQAANTRTTASIGGQNILGSGTVRAATGPSAGGGTASAEVEGLSLLGASTIGADAVSTTCLMTASSISGDTDAVNLNIAGNAVNPTANTNISIPSVAGVVVDRQTATYASSTGRLTYTVRGLDVSLLGGGLAGVASGSVVVAESTCSGIVNLSAITTSGRTIVPGNSGTPTVNVTNNGDIAAPNTVITIPAPPSGYTLGTVTTTGGGTCTSSTTNVRCTGVTVPGAGSVTVSLPVTVASSGADASNWAPSSGAITAISTPVAAVPGTTLSVTGNGTLVTVGAPQTTGGTITVTPMTVPAGKNASTPITVTNQGPSDATTTVTIPISGRPAGLSVMSATVGGNSCGVTSTAITCTGVTVPANGSTAISVRVAATTTTAPGVTWAVSGITATLNGRALSGGGQLVTVSDPDVNFTNGVTITSDTGVPGGPSAVPSVKVTNVGVVAATDAVITLPAPPAGYTVGTVTTTGGGTCTSTTTFVRCTGVTVPAMGSTTVSVPVRLASNVTADWVASTGAAVTATSGDSTGTATGPLVTANPGWTLSAEATGPAPNTVSAGQDTSMQVVVTNEGPSDATGAVFTVIAPGSTTFRTLTGTAAQVCTQTAPGALRCTVSLVADADLTLALPLTVSAQASTSAPLSGGCVSFNGDGDCADADDVALPDLTLRTPIGGRLDVRFTPPTITPGSTGPSRITLRSTRAESGLTVTVPTGGLPTGMRVTTSSVPSGSCTTGASQVSCTGISLTANQAIDLTLTVEVASSVTAGTRWIADGIVVADGPEQLTYNNITLAITGDPVVDVTATATGPAPGTVQPGGNGNLTVVVSNAGPSDATDYTFTVLAPTSTRFDTLAAPAATYCTLSSDTRAVCTVDLADDTSTPGLVFRLLVDASADPYTPLAGGCVDLDGRNGCGTNDTTVTPIVLGAPLDRRLTVTTDPVTVTPGRQDVGHIELTATRGDVAGLTVTVPLDDLPAGLTLITQSATSGSCTLGVGQVTCSGINVLNGATAFVNLTVRAAPGVAQGVRWTVPDITVAQGADSITFRGQLAVTGAPDYTLDVDVTPPAGPVTPGGPGADLTVDLDNTGPSDARAVTISVLAPDGSTFGPLTAPTSGSCRLDSPTRAVCTQDITVAVNPRYVFRLLVSGGVDPNQPLTGGCVDVNNNGVCGPAPDTGFPAIPLTLSFDRQIDVDTVPAVITPGNTGTAQVRLTATTALSNLTVTIPLAGKPPAITVVPLGLAPLCTLDLVTLNAIVCTNVSVPANGSTVIPIPVLVLPGAATTLVWSSRALTVENTAGDTATGAGILVRTGTPVYTLTAGITGPAAGTVVPGSTTSMRVVLTNTGPSTATDAVVTLRAPVGTGFGDLTAPMTNSCQRVSSSRITCAVTIAVNGTLTWDVPIVIPGNADPSVNLTGGCVDLDGNGACGGVADQVMPDIILRAQMGRIATISAVNTPITPGSSTTAPITVRLSEARDDVTVTIARDTLPAGVTLTGITGLPCTSTASAFTCGDFDFAANGSVTLTLALTATAAAVPGAVWTPTVTVAQSGDSVSRGVSVLTVAPDDARLAVTIQVPAPGTMMPGGTGDLTITITNSGPSTARGARYSFRAPAGTTFLALTSPVADYCVRAAADRVDCTVDVPGNSNLQFVLKIQVPASTGTDQPVGDGCVDTNRDGDCNDPTDQPIPDIELGSPLSGDITLNTQVGTGTPGLTATGTVTVAAEKAMTGITITVPLATVPAGFTVTGASGPTGSTCTLATDKITCTGVNVVVGTNTVASVTVKLASSLTAGVTWTAYAIAAARNGETVQTSGAIVTTGPPQPNVTSTVTLPSGPVTPGTTTTMTVTVTNAGPSDAVNQVALIYAPGQTTFGPLSGRAATDCSLLSTTRVSCQFDLAANATPRVWVLPVVVNSAADPTKPVAGGCVALNGDTTDCDELAVGLPLRNTTTVALNQVTIAPGESGTPTITIDSTTERESLSLVVPLEALPDEFTVTGAETDASPGQCDVAETEVSCTDMPVDAGDTITVALTVEVAGGAASDLVWTAGSVTLTRDDDANDQLISSGKLVTTGVTGDNVTTTFGRLTVNPAAPGQTTRLPVRWKNTGDTDADPHVMVLVIPDGLTPGTPLPSGCVWDEGTNSITCTRPIGAGETLSFSIPLVVGTEIAAGTVISGGCLDADPANYTCGDAGDQALPTLSVVAPKVDLVLGVKKKTTSAARGGLALIKLQYQNNGTESAADVSFLIDPPRGVTLASARILLDAFDDGGATVAAECGPADTGDDNTVSCVGPEAAVGTKSELWLYLRIASTAPSGTYPVRVTISTASAEGNVVNNTVEALLAIAGAPDDNSNSGGSGGDNLPTTGQNLVGLLILSVLLVIAGVMLRVGARRGRQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUR9</accession>
    <name>U5VUR9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein yycF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11475"/>
    <dbReference type="EnsemblBacteria" id="AGZ40584">
        <property type="protein sequence ID" value="AGZ40584"/>
        <property type="gene ID" value="AFR_11475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2339"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13213" checksum="F2BF35BC564A0AF1" modified="2014-01-22" version="1">MTVVLVADDDADIRDLVAFKLEQAGLEVIAVEDGQAAVEQARAKLPTLAVLDVSMPGLSGIDVCRMLRADPSTAGMLIIMLTARVQEQDVEGGFSAGADDYVTKPFSPRELVSRIQALLSRARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUT5</accession>
    <name>U5VUT5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mannose-1-phosphate guanylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358947.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05635"/>
    <dbReference type="EnsemblBacteria" id="AGZ39416">
        <property type="protein sequence ID" value="AGZ39416"/>
        <property type="gene ID" value="AFR_05635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1143"/>
    <dbReference type="eggNOG" id="COG0836">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035527_0_1_11"/>
    <dbReference type="OrthoDB" id="224217at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005976">
        <property type="term" value="P:polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001538">
        <property type="entry name" value="Man6P_isomerase-2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01050">
        <property type="entry name" value="MannoseP_isomer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="MannoseP_isomer" evidence="2">
        <location>
            <begin position="330"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="39637" checksum="4C476156D33EA10F" modified="2014-01-22" version="1">MSENPGELYAVVPAGGSGTRLWPLSRAGHPKFLHPLTGTDASLLQATVERLAPLADPSRVLIVTGVAHAAAVARQLTELPAENILVEPSPRDSCAAIALAAAVIARRDPEAIMGSFAADHLIAENDRFINVIRQAMTGARQGLLMTLGITPTRPETGYGYVQCGGPVGDGPVLSVEEFKEKPLYDVAESYVKSGNYLWNAGMFVWRVDVFLSELARQQPQLHAGIARIAQAWDSPSREEVLGEVWQTLPRISVDYAVMEGAATVGRVGTVPGDFGWNDVGDFHTLGEVLTADSGGNVVVGSDVLNTPSVLLRETENLVVVPNSGRLVAALGVRDLIIVDTPDAVLVCPRNRAQEVKSLVDELKSKGDLHYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV91</accession>
    <name>U5VV91_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Spermidine/putrescine import ATP-binding protein PotA</fullName>
            <ecNumber evidence="8">7.6.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">potA</name>
        <name evidence="10" type="ORF">AFR_06655</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.11" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359151.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06655"/>
    <dbReference type="EnsemblBacteria" id="AGZ39620">
        <property type="protein sequence ID" value="AGZ39620"/>
        <property type="gene ID" value="AFR_06655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1358"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_11"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015594">
        <property type="term" value="F:ABC-type putrescine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03300">
        <property type="entry name" value="ABC_PotA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017879">
        <property type="entry name" value="PotA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005893">
        <property type="entry name" value="Sp_pt_ABC_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01187">
        <property type="entry name" value="potA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="17"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="53"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="377" mass="40983" checksum="3C02D7FB7067C46D" modified="2014-01-22" version="1">MSIVNEDPGDGTAQPAIELAGVHKDYLSHGEKVQAVRRLDLTIGSGEFFSLLGPSGCGKTTTMRMIAGFEEPTGGTVFLDGQDVTRVSPNKRDVNMVFQSYALFPHLNAYQNVCFGLERKKVAKKEIQRRVGEIMEIVSLNGMEKRAPREMSGGQQQRVALARALVNRPRALLLDEPLGALDLKLRQQMQVELKRIQREVGITFVYVTHDQGEALTMSDRIAVMESGRIDQLGSPREIYEKPATRFVAGFIGTSNLLDGSIDSITEGVATLSYGEGERVLVPVVGAMKTGDTVEVSIRPEKIDLSQDAPAGTGDSVLTGVVTDVVYHGTSTNYTVKTSAGPDFVVFDQNAFSAEDLAGPGDRVFLTWNCQHSYPIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV96</accession>
    <name>U5VV96_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12565</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360858.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12565"/>
    <dbReference type="EnsemblBacteria" id="AGZ40799">
        <property type="protein sequence ID" value="AGZ40799"/>
        <property type="gene ID" value="AFR_12565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2552"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="33043" checksum="C0D0E6551612B3B6" modified="2014-01-22" version="1">MSIVDVQGLRKTYGAVTAVDDVSFTVAEGEVFGILGPNGAGKTTVVECVEGLRIPDAGTIRVAGFDPHRQRDEVTRILGVQLQASDLQPKLTVQETLEMYSAFYAGPADWRPLVERLDLEEQLSRRFGKLSGGQKQRLFIALSLLGNPRVVVFDELTTALDPRARRRTWELVRDINASGVTILLVTHFMEEAQYLCDRVAVFDRGEVVALGTPDELISSSAGAIVISFRLSEPLPEGKLESLPGTVSVAYEGAKVVVRGSDDTVDALISMIVRERISAQGLRIVDATLDDAFLSITANAEAPEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVD5</accession>
    <name>U5VVD5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13410"/>
    <dbReference type="EnsemblBacteria" id="AGZ40968">
        <property type="protein sequence ID" value="AGZ40968"/>
        <property type="gene ID" value="AFR_13410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2723"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840807_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="1">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="14528" checksum="C8381611CAD30FFC" modified="2014-01-22" version="1">MTAEDQIAVSGTLDGGAILSVHHRGGMPSGAGFSLTIDGTDGRLEVTSGEFPHLGPVTVHGTRGGGRPEKMTLPAGYDTFPGLSGTVIHTLAHAYAAIRDDLVHDTAVAPDFTHAVQRHRLLDAIVRSAATGRRTAVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVK3</accession>
    <name>U5VVK3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_06835</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06835"/>
    <dbReference type="EnsemblBacteria" id="AGZ39656">
        <property type="protein sequence ID" value="AGZ39656"/>
        <property type="gene ID" value="AFR_06835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1396"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_0_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="17114" checksum="2C0A805BF10AFAAF" modified="2014-01-22" version="1">MMAHLDELDTAILRELQVDARRTNRDIAAAVGVSPTTALDRTRALRERGVIQGASLTVDLAAIGRPVQALIAVRIRPPSRRNIEAFRNWVTALPDTLGVFVTTGTEDFLIHVAVPDNESLYAFVIDRLTEHAHVADVRTSVVYEHLRNDRITPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVK7</accession>
    <name>U5VVK7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD_binding_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360963.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13090"/>
    <dbReference type="EnsemblBacteria" id="AGZ40904">
        <property type="protein sequence ID" value="AGZ40904"/>
        <property type="gene ID" value="AFR_13090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2658"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_14_2_11"/>
    <dbReference type="OrthoDB" id="867226at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="11"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="542" mass="59138" checksum="32D0B6C661FE28B6" modified="2014-01-22" version="1">MRHAGDHPEKVGVLIVGAGPTGLTLGIDLAQRGVPCLIVEATTERPVNPRCNTTSARSMEIFRRLGVADGIRRAGLPLDYPTSIQYRTTLRGTELFRLTFPSSGEVLDGAGRADWPTPEPQHRISQLYLEPILEEHARRTTGLTLERDCRLVELRPHADHVEAVVESGGQTRVLHCDYVVGCDGAHSAVRRGLGVRYQGVEAIQKFVSTYLRSADLGRLAARDPAWTYWMFGRVQSSLIAIDGGDRWLHHVAFAPDHDTDAEDPGKLLDEALGGPVEHEVLGVVRWTGRQLVAERYQDDRVFLAGDAAHIWIPVGGFGMNTGIQDAATLGWMLAAVYRGWAPPELLRAYELERKPVGEQFAGAVGRAARASLAEVSPDIHIPGEAGDRARHEFAHRLATTEPHRYSPDGFSFGYHYAHSPLVLDGGEQPAIEMGVYDRRARPGFRLPHTWLPDGTPVLDALGREFTLLRTAGGDADAFKAAAAALGIPLTVLDLGSRWPERYPADFILVRPDQHVAWMGDAATRPGDVLAAATGRLLVSPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVM5</accession>
    <name>U5VVM5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative trehalose-6-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07380"/>
    <dbReference type="EnsemblBacteria" id="AGZ39765">
        <property type="protein sequence ID" value="AGZ39765"/>
        <property type="gene ID" value="AFR_07380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1505"/>
    <dbReference type="eggNOG" id="COG0380">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_7_1_11"/>
    <dbReference type="OrthoDB" id="556566at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="PTHR10788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="461"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="492" mass="54906" checksum="2F2AD7C3F17934FE" modified="2014-01-22" version="1">MAERSSFVVVANRLPVDEVMVDGERQWRPSPGGLVTALHPVLAEQRGTWIGWAGGDGEAPEPFELEGIKIHPVPLSAEELERYYEGQSNATIWPLYHDAVETPVYKRRWREAYRLVNQRFAQAAADVADEGATVWVQDYQLQLVPAMLREMRPDLRIGFFLHIPFPPIELFMQMPFRAEILRGLLGADLVGFQQRLAAQNFVRLARHLLGLRYEGQSIQVDGRKVKAGAFPISIDTADMEKMAADPAVQARAKQIRAELGDPETVILGVDRLDYTKGIELRLKAFRELLADGKLKVPEAVMVQVATPSRERVEHYQTLRVKVEREVGRINGEFGRVGMPAVHYLHQSYSKQELAALYVAADVMMVTPLRDGMNLVAKEYIACRTNGGGALVLSEFAGAATELRQSFLCNPHDPDGVKDALLRAVGAEPAELKRRMRVMQRHLRTHDVAEWARTFLNELGVPETDASDSAPEKDAPARNDAASETEAAESSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVU2</accession>
    <name>U5VVU2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361053.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13540"/>
    <dbReference type="EnsemblBacteria" id="AGZ40994">
        <property type="protein sequence ID" value="AGZ40994"/>
        <property type="gene ID" value="AFR_13540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2749"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_11"/>
    <dbReference type="OrthoDB" id="1261526at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="238" mass="25296" checksum="2B8F573D4D9943AF" modified="2014-01-22" version="1">MTDSKTILITGASSGIGAATARRLADQGHTVVLGARRVDRLEAIRDAGGDVHIDRLDVTDRADVTRFVDAAVSTHGRIDVLINNAGVMPLSMLDALLVEEWDRMIDVNIRGLLHGIAAALPHFQRQGSGHFITVASIGAHEVSPTAAVYCGTKYAAWAITEGLRREVDPSVRVTTISPGVVESELAESISDPVARAEMKAYRANSIPADAVARAAAYAIGEPDGVDVNEIIVRPAMQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW27</accession>
    <name>U5VW27_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359466.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08230"/>
    <dbReference type="EnsemblBacteria" id="AGZ39935">
        <property type="protein sequence ID" value="AGZ39935"/>
        <property type="gene ID" value="AFR_08230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1679"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061796_1_0_11"/>
    <dbReference type="OrthoDB" id="1169054at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="22"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="335" mass="37819" checksum="221F864276293A62" modified="2014-01-22" version="1">MARPITLFTGQWADLPFEEVCRLASEWGYDGLEIACWGDHFEVDRALAEDDYVDRKREQLAKHNLQVFAISNHLVGQAVCDHPIDERHQDILPPSIWGDGDAEGVRGRAAERMKDTARAAAKLGVDTVVGFTGSSIWHTVAMFPPVPPAMIERGYQDFADRWNPILDVFDEVGVRFAHEVHPSEIAYDYWTTRRTLEAIGNRPAFGLNWDPSHFVWQDLDPVNFILEFADRIYHVDCKDAKVRTGDGRRGRMSSHLPWADLRRGWDFVSTGHGDVPWEDCFRALNAINYTGPLSIEWEDAGMDRLVGAPEALQFVRRLAFDAPSAAFDAAFSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW88</accession>
    <name>U5VW88_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative 2',3'-cyclic-nucleotide 2'-phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_08555</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a nucleoside 2',3'-cyclic phosphate + H2O = a nucleoside 3'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19621"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:66949"/>
            <dbReference type="ChEBI" id="CHEBI:66954"/>
            <dbReference type="EC" id="3.1.4.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 3'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10144"/>
            <dbReference type="ChEBI" id="CHEBI:13197"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.1.3.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08555"/>
    <dbReference type="EnsemblBacteria" id="AGZ40000">
        <property type="protein sequence ID" value="AGZ40000"/>
        <property type="gene ID" value="AFR_08555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1740"/>
    <dbReference type="eggNOG" id="COG0737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005854_4_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008663">
        <property type="term" value="F:2',3'-cyclic-nucleotide 2'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008254">
        <property type="term" value="F:3'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07410">
        <property type="entry name" value="MPP_CpdB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006146">
        <property type="entry name" value="5'-Nucleotdase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041827">
        <property type="entry name" value="CpdB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="PTHR11575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="SSF55816"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00786">
        <property type="entry name" value="5_NUCLEOTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005147821" evidence="7">
        <location>
            <begin position="22"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="8">
        <location>
            <begin position="39"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="5_nucleotid_C" evidence="9">
        <location>
            <begin position="365"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="589" mass="63284" checksum="71BCDE8F3718FDFB" modified="2014-01-22" version="1">MLGAAAAGVAVPLVLGEAAQAAPSHGHGHGHAPKTYDLTVLGTSDTHGNVYNWDYYKDLEYDDSAHNDVGVAKLAALVNQIRAERRGKATLVLDAGDTIQGTPLATYYAKQEPITTTGEKHPMARAMNVLHYDAITLGNHEFNYGLPLLNLWIRQLGFPALAANAVDAKSGRPAFTPYVIKKVSLGHGAPTLRVGILGLTNPGVAIWDRANVEGKLDFEDMVATAAKWVPVMRARGADIVLISAHGGDSGTSSYGPELPNENPSALIAEQVPGIDAILFGHAHANVPQRFVTNTKTGAQVLLSEPSKWGQRLARMDFTLTRDRGRWTITAKKSDLLNTNTVAEDPKVLAAVRSQHAKTVAYVNQVVATSTEELSAAESRYKDTPILDYINKVQTDTVTAALAGTEYASLPVLSIAAPFSRTAVFPQGDVKIKDVAGLYIYDNTLEAVVLTGAEVKAYLEFSAKFFVTLPVGATPDPATISDPAVPDYNYDVFSGVDYDIDISRPVGDRITRLEIAGVAVAADARFVVAVNNYRRSGGGNFPGIVKTQVYNAQQEIRQLLIDWAQAEGEINPADFFVPNWQLVRAGVPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWE1</accession>
    <name>U5VWE1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ros/MucR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361146.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14005"/>
    <dbReference type="EnsemblBacteria" id="AGZ41087">
        <property type="protein sequence ID" value="AGZ41087"/>
        <property type="gene ID" value="AFR_14005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2841"/>
    <dbReference type="eggNOG" id="COG4957">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968497_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041920">
        <property type="entry name" value="ROS/MUCR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008807">
        <property type="entry name" value="ROS_MUCR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05443">
        <property type="entry name" value="ROS_MUCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="31153" checksum="1D57BFBED4EF828D" modified="2014-01-22" version="1">MICALSRPEAGVPEARRRRAASDSGHGHLWQLPDGTGLHARPAELTVDDATGRLCCHLCGRWYTSLGSHVRAHGHTADSYRSTLGLCAGEPLTARGLSAAIRDRQARRYRSNTELRDAFAAGAAQLRHTRDASARLEEPEQRVTRRRAALQAGRETTAARRRRELDDRLTRLGHPDLAGYLRSAYAAGAGLQALAAATGLGRARLRAALDVAGVAVRPTGVNTAEGRRSRALSADRAAAVRVGTDDLHAWLGRRRTEGWSLVRLAAAVGHSTHWVRWRLDKTDVRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWY3</accession>
    <name>U5VWY3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bac_luciferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359894.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09680"/>
    <dbReference type="EnsemblBacteria" id="AGZ40225">
        <property type="protein sequence ID" value="AGZ40225"/>
        <property type="gene ID" value="AFR_09680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1973"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_6_0_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="30818" checksum="2375DAEA0D84942D" modified="2014-01-22" version="1">MTVFGFGLDHTRALLDQAVQADRDGLDLVSVSDHPYLADRPDAYAAIGVLLGRTTRISGLVNVSNLPTRPAPMLARMVSGLSTLTGGRVVLGLGAGGLLADAARLGVRQLSPAEAVRALEEAMVVVRALFGGGAPVTFDGEFYRVTELAPADVPAPRIWTGSVGPRSLAVTGRHADGWIPGHAADWLSDRFRTSRPIIDEAAVAAGREPAHVATIYNLTGRITAGPLPVTRDPSGRWIGGSAAQWAEELTGAVLDHGAGGFTYFPDDDGTPIGVALGRWAREVVPEVRAAIGAAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX87</accession>
    <name>U5VX87_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Copper binding protein, plastocyanin/azurin family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_15625</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361466.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15625"/>
    <dbReference type="EnsemblBacteria" id="AGZ41407">
        <property type="protein sequence ID" value="AGZ41407"/>
        <property type="gene ID" value="AFR_15625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3173"/>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023405_0_0_11"/>
    <dbReference type="OrthoDB" id="1452706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028871">
        <property type="entry name" value="BlueCu_1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022038">
        <property type="entry name" value="Ig-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12245">
        <property type="entry name" value="Big_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00196">
        <property type="entry name" value="COPPER_BLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665813" evidence="1">
        <location>
            <begin position="24"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Copper-bind" evidence="2">
        <location>
            <begin position="46"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like_bact" evidence="3">
        <location>
            <begin position="309"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="498" mass="50691" checksum="B27ADEC8DAC62753" modified="2014-01-22" version="1">MRRFLLSLLLVLSPVVVASPAAAAQTLTWTADDDITRYKSTPSSAVAGATTIVFENSAATGNTTGMPHTLTFDTTAEGYNHDVSLNILASPFDAQDGRHQQAVTLTPGRYRYFCSIPGHSTMVGELVVTGGGGDPDTTAPTVSAQVSGDQNPDGDYLGTATVAVSASDAGSGVDTIEYQVDDTSFQAYTAPVAVTAVGDHSVQFRATDNAGNVSAVGSVSFSIVESAPGDTTPPVVTSQVTGDRDTQGRYLDRATVTLAATDTGSGVARIEYNVDGGAFAVYSAPVVVTGAGSHMVHFRATDVAGNTSAEQMASFTVVAAPVPDTTAPVTAATANTSQPGRAVVTITATDAGSGVASIEYALDAGAWAAYTAPVTVTAVGAHTMRYRATDVAGNVAAVASITFTVAAPPTGDCPVPDTRATVVIGTEDTGIPNTDTGNGCTINDLIDEHAGYPNHAAFVRHVETVTTPLVAAGQLTARQQGTIVRAAARSEAVKGVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXL7</accession>
    <name>U5VXL7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Flagellar biosynthetic protein FliQ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fliQ</name>
        <name evidence="10" type="ORF">AFR_10610</name>
        <name evidence="11" type="ORF">AFR_19185</name>
        <name evidence="12" type="ORF">AFR_31095</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Role in flagellar biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the FliQ/MopD/SpaQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360261.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10610"/>
    <dbReference type="EnsemblBacteria" id="AGZ40411">
        <property type="protein sequence ID" value="AGZ40411"/>
        <property type="gene ID" value="AFR_10610"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42109">
        <property type="protein sequence ID" value="AGZ42109"/>
        <property type="gene ID" value="AFR_19185"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44479">
        <property type="protein sequence ID" value="AGZ44479"/>
        <property type="gene ID" value="AFR_31095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10610"/>
    <dbReference type="KEGG" id="afs:AFR_19185"/>
    <dbReference type="KEGG" id="afs:AFR_31095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2163"/>
    <dbReference type="eggNOG" id="COG1987">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164516_2_0_11"/>
    <dbReference type="OrthoDB" id="1939288at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002191">
        <property type="entry name" value="Bac_export_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006305">
        <property type="entry name" value="FliQ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34040">
        <property type="entry name" value="PTHR34040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01313">
        <property type="entry name" value="Bac_export_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004669">
        <property type="entry name" value="FliQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00952">
        <property type="entry name" value="TYPE3IMQPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01402">
        <property type="entry name" value="fliQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ42109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ44479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="89" mass="9561" checksum="E9A2091E726C22A0" modified="2014-01-22" version="1">MTDTKIVELGLQAMTISAKMCAPVLITALLIGFAISLFQSVTQIQEATLSFVPKAVGVGAAILFSGNWMLHEMVTYTTQLFENIPSLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXU6</accession>
    <name>U5VXU6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11035</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11035"/>
    <dbReference type="EnsemblBacteria" id="AGZ40496">
        <property type="protein sequence ID" value="AGZ40496"/>
        <property type="gene ID" value="AFR_11035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2248"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="3">
        <location>
            <begin position="197"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="398" mass="42436" checksum="4612C4250BED20A3" modified="2014-01-22" version="1">MEVVGALRRVVSGPDRPPAPVLSRRLTKIQVYAVPLGLLALLGAGLANGQYLLENRSTGQFIAVVLSVWAVLPVLLAFRRPLLAWRISYPLLFVGALGKGPREAWPWTTVQIIGFLIVLILLVRREEPGVVAWVTALNIVPLFLLAPWTNAWGVTVWFIAVALAGDLVSRRRRSREALAQQTELTELEKARRAVLEERARIAREMHDVVAHHMSMIAVQAETAPYRITGLPEPALAEFTTIASGAREALTDMRRLLGVLRAEDSDTLLAPQPGLSDVGALVETARRAGVTVRLETEGIEHVPEAVGLAAYRIVQEALANAARHSPGGPVHVAVRGSAGLLRLEVGNGPGSPAVAAGGPGHGLIGMRERAALLGGTLEAGPQPGGGYRVDARLPLGEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY96</accession>
    <name>U5VY96_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycine betaine/L-proline ABC transporter ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362341.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18450"/>
    <dbReference type="EnsemblBacteria" id="AGZ41968">
        <property type="protein sequence ID" value="AGZ41968"/>
        <property type="gene ID" value="AFR_18450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3744"/>
    <dbReference type="eggNOG" id="COG4175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_2_2_11"/>
    <dbReference type="OrthoDB" id="1713578at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031460">
        <property type="term" value="P:glycine betaine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005892">
        <property type="entry name" value="Gly-betaine_transp_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01186">
        <property type="entry name" value="proV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="24"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="61"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="344" mass="37582" checksum="1D36C23A09E47E89" modified="2014-01-22" version="1">MTPTIAVEHLWKVFGPKPDRIVGTPLADLSRADLRAQTGCLAAVKDVSFDVAPGEVFVVMGLSGSGKSTLVRCLTRLIEPTSGEVRINGESVREMNPRRLRELRRHGVAMVFQHFGLLPHREVVDNIAYGLEIQGMSKADRHARAGEVLSLVGLDGHEHQYPHQLSGGMQQRVGLARALATDPAVLLFDEPFSALDPLIRRDMQAEVRRLHAEVGKTMVFITHDLAEALSLGDHIAVMRDGELVQVGTPEELVGAPADKYVSDFVSEVPRADVLSVRWIVRPAKDDEEIEDQPFPAGTVIRDAVPRVLRSAHAIRVVDGDEVLGVVDAEQLVPALIGHRPQVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYJ3</accession>
    <name>U5VYJ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_17870</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 3">Glycan metabolism; L-arabinan degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17870"/>
    <dbReference type="EnsemblBacteria" id="AGZ41852">
        <property type="protein sequence ID" value="AGZ41852"/>
        <property type="gene ID" value="AFR_17870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3625"/>
    <dbReference type="eggNOG" id="COG3507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009397_5_1_11"/>
    <dbReference type="UniPathway" id="UPA00667"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046558">
        <property type="term" value="F:arabinan endo-1,5-alpha-L-arabinosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031222">
        <property type="term" value="P:arabinan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016840">
        <property type="entry name" value="Glyco_hydro_43_endo_a_Ara-ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026534">
        <property type="entry name" value="Endo_alpha-L-arabinosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666218" evidence="7">
        <location>
            <begin position="33"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="175"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="195"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="6">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate recognition" evidence="6">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR026534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR026534-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR026534-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR026534-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="35602" checksum="0149997D73664D78" modified="2014-01-22" version="1">MSRTRTGRALWLGVATGALLAVAAVASVNAEAAVPVVAAATPTVPPAAYPNPGVVTGDTGTHDPSMVKTPAGGYLAAYTGDNVQLKTSPDRTAFRNAGAVFPGGASWTTAYTGGSRNLWAPDLSYRNGRYYLYYSASTFGSNHSAIFLATSTTGASGSWTNQGLVIESRTSDTFNAIDPNLIVDAQGRWFLSFGSFWSGIKQIALDPATGKRSGTSLRAIAGRNGGAIEAPTIFRHGNYYYLWVSFDLCCRGASSTYRIMVGRSVSVTGPFVDRNGTAMTAGGGTQVLASHGSIHGPGHQAVLADTDSDVLVYHYYANSGASYLGINLIGYDQAGWPFAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYK2</accession>
    <name>U5VYK2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17920"/>
    <dbReference type="EnsemblBacteria" id="AGZ41862">
        <property type="protein sequence ID" value="AGZ41862"/>
        <property type="gene ID" value="AFR_17920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3635"/>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="130" mass="14378" checksum="793D4880C0C9850A" modified="2014-01-22" version="1">METAGQTRAQACGQLPVEHGDFVREVIDRVGDKWTLLVIANLRDGPRRYSQLQQLVPGISQRMLTLTLRQLKDDGLVTRTAYAEVPPRVEYALTSLGASLLDTATALIDWATANHAAIRDNRLKRPSGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYP6</accession>
    <name>U5VYP6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fido domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12500</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360845.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12500"/>
    <dbReference type="EnsemblBacteria" id="AGZ40786">
        <property type="protein sequence ID" value="AGZ40786"/>
        <property type="gene ID" value="AFR_12500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2539"/>
    <dbReference type="eggNOG" id="COG3177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061806_0_0_11"/>
    <dbReference type="OrthoDB" id="1232677at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040198">
        <property type="entry name" value="Fido_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504">
        <property type="entry name" value="PTHR13504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="SSF140931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fido" evidence="4">
        <location>
            <begin position="107"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="197"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for autoinhibition of adenylyltransferase activity" evidence="3">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="379" mass="42415" checksum="563F1F933A89BF54" modified="2014-01-22" version="1">MLIPTPSLTAADRVVLASVDEMRDRLRHQVQDRPGKSTSGLRKFLTADAVAASNSIEGFRVSTQDVQDLMDGERDVDVSDENRRETLAYELMMTYVQSLHDVADFEYSKGLLNALHWMLQGHRHARNKPAGQWRAGPVYVTDPRDPLVAAYTAPAASAVPELMAELVAWLNADSDTHPLIRAAMAHLHLVSIHPWADGNGRMSRSLQTLLIAREGVLAPEFSSIEAWLGRPGNTWEYYQELQRRGATYQPDQDVSGWIRFNLTAYHQQAQTVQHRMDRSAAVWELLATFAGKSGLDERTISALHDVAVAGRVRRTRYEQAEGLTLQQAQRDLRDLATARLLVPVGRTRARYYTEGPQFPDSVLELARTPFSLASPYTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYU0</accession>
    <name>U5VYU0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18370"/>
    <dbReference type="EnsemblBacteria" id="AGZ41952">
        <property type="protein sequence ID" value="AGZ41952"/>
        <property type="gene ID" value="AFR_18370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3728"/>
    <dbReference type="eggNOG" id="COG0626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044561_0_0_11"/>
    <dbReference type="OrthoDB" id="1695669at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="500" mass="54497" checksum="A0E9E384F5E9B66E" modified="2014-01-22" version="1">MGVRRHRDPAGVMTAGTQAAPATGRRHRDELSLERELLTGLAALAEAQSAGDERQWARFRARLAAIDVAADPLAAEHRLETASEQLRALLLRRGHRLDEEHFRSPDSTGDRRLPSGLAFPHSYERGLPVAHLESRLASTPPDGWSGAVVTFNSCMAGLGHVLQSLVHMLRPDERRPLSVAYWGDYFETDLLMEYLGSAVLRYDKVGVGDLTETLQRGGHDVLLIEPVRYNWELDVLDVSALLRAWRSAPDRPRIIVVDTTLVSPLWPTRAFLDGLTGLGDVLVVELRSGLKLDQQGLELANLGIAEIYSHAGPPTADQLASTLRLARSTTGSGIAVAAAAALDTGFVADPAWTRRHAGQVFATNARLARELAEVRGIFAEVVHPALRPGTPGLAQSPFVIVKLAEDTLENHGLLLAALRREVARRGIRFTHGSSFGFRSHRFETIVPRVARREGLFKIAAGSRSGPFLDIITDVLRDLGGLSGMAALRARFPDLKAIPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYX7</accession>
    <name>U5VYX7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18900</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362427.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18900"/>
    <dbReference type="EnsemblBacteria" id="AGZ42054">
        <property type="protein sequence ID" value="AGZ42054"/>
        <property type="gene ID" value="AFR_18900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3839"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_5_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="13"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="106"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="175" mass="19633" checksum="E88FD40A00D5EA54" modified="2014-01-22" version="1">MRDTDHERAFRRFFEQHHADLSRLAYLVTGESGVADDLAADAFVEVWKHWERVEAADSPIAYARGILTNLARQWIKQRSRERLGLLGLGLLRRDRSEPDTPVVLDVRAALRHLPLRRRECVVLRYAFDVPEREVAEILGISVGAVKSLTSRGAAQLSEHLMDLPVRTGGLSASAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZA7</accession>
    <name>U5VZA7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362581.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19685"/>
    <dbReference type="EnsemblBacteria" id="AGZ42209">
        <property type="protein sequence ID" value="AGZ42209"/>
        <property type="gene ID" value="AFR_19685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3995"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_12_3_11"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="28263" checksum="E358E8EBBBE69088" modified="2014-01-22" version="1">MQVVLTGGTGYIGVALIKELVGAGHQVTALVRNEAGAAKVTALGAQATVGDLFDSAWVAEQFAAGDAVAHLAATGDATTQELDRGVVAAAVRAGKPYVHTSGIWIWGSNDTITEESPFAPPQLTAWRSAVEKLVLQADLTATIIAPGIVYGYGGGIPGGVFDGSGKLVGQGTQHWTTVHVDDIAALYRTVLERGEGLGYLIGASGENPTVRELGEAAAGNAGVTPESVDASRERLGALFADALLLDQQTTGAKARSLGWAPKAVSLLDDLRSGTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZP2</accession>
    <name>U5VZP2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_20435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nlpA lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362731.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20435"/>
    <dbReference type="EnsemblBacteria" id="AGZ42359">
        <property type="protein sequence ID" value="AGZ42359"/>
        <property type="gene ID" value="AFR_20435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4144"/>
    <dbReference type="eggNOG" id="COG1464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067080_0_0_11"/>
    <dbReference type="OrthoDB" id="1230456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004872">
        <property type="entry name" value="Lipoprotein_NlpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30429">
        <property type="entry name" value="PTHR30429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03180">
        <property type="entry name" value="Lipoprotein_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002854">
        <property type="entry name" value="MetQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665526" description="Lipoprotein" evidence="6">
        <location>
            <begin position="31"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-diacylglycerol cysteine" evidence="5">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR002854-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="272" mass="28831" checksum="E331710A1924834B" modified="2014-01-22" version="1">MRHRSFAASLAAAGLVLSLAACGGSDSGSAADDTLKVGVSPVPHAEILNYVKDNLAAAEGLKLEVVEFNDYIQPNVALSEKQIGANYFQHIPYLDEEVAAKGYKFTALKPVHIEPLGLYSKKVKTLAEVPAGGVVALPNDPSNSGRALNLLAKNGLITLKDGVGVKATEKDIATNPKNLSFKSLEAAQLPRSLEDTAVSVINGNYAIQTGLQPAKDALALEDGQDNPYANLLVVRTGDENDPRVVKLEKLLHSAEVKKFIADKYQGSVLAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZQ8</accession>
    <name>U5VZQ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bifunctional D-altronate/D-mannonate dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362746.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20510"/>
    <dbReference type="EnsemblBacteria" id="AGZ42374">
        <property type="protein sequence ID" value="AGZ42374"/>
        <property type="gene ID" value="AFR_20510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4159"/>
    <dbReference type="eggNOG" id="COG4948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_6_1_11"/>
    <dbReference type="OrthoDB" id="1825548at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:cellular amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034589">
        <property type="entry name" value="D-mannonate_dehydratase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="Mandelate_racemase_DgoD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="PTHR48080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080:SF6">
        <property type="entry name" value="PTHR48080:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00033">
        <property type="entry name" value="mannonate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00908">
        <property type="entry name" value="MR_MLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00909">
        <property type="entry name" value="MR_MLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MR_MLE" evidence="1">
        <location>
            <begin position="128"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="402" mass="44040" checksum="2AC0BEFDE74F2FA2" modified="2014-01-22" version="1">MRIAEARVIVTCPGRNFVTLKLVTDDGLTGVGDATLNGREQAVAAYLTEHVVPVLLGRDPARIEDTWQLLYKGAYWRRGPVTMSAIAAVDTALWDIKGKVAGLPVHELLGGRSRDGVTVYGHASVSSLDGILTDVGRLVDQGYRAVRVQAAIPGLPTTYGVSADARTYEPAGSALPAQEVWSTPRYLAHVPGVFERVRAEFGPDLRLLHDAHHRLTPIEAATLGRRLEPYALTWLEDPVPAELQEGYRLIRQHTTTPLAVGEVFNSIWDCQELITGQLIDYIRASVVHAGGISHLRRIFDLAHLYHVRSGSHGATDLSPICLAAALHLDVSIPNFGLQEYMPHTAATDEVFPHGYHYEDGYLHPSTTPGLGVDIDEELAARYPYRPASLPVNRLEDGTLHSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0G3</accession>
    <name>U5W0G3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21250</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IucA/IucC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21250"/>
    <dbReference type="EnsemblBacteria" id="AGZ42522">
        <property type="protein sequence ID" value="AGZ42522"/>
        <property type="gene ID" value="AFR_21250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4309"/>
    <dbReference type="eggNOG" id="COG4264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018524_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007310">
        <property type="entry name" value="Aerobactin_biosyn_IucA/IucC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022770">
        <property type="entry name" value="FhuF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037455">
        <property type="entry name" value="LucA/IucC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34384">
        <property type="entry name" value="PTHR34384"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06276">
        <property type="entry name" value="FhuF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04183">
        <property type="entry name" value="IucA_IucC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IucA_IucC" evidence="3">
        <location>
            <begin position="236"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="IucA_IucC" evidence="3">
        <location>
            <begin position="291"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="FhuF" evidence="4">
        <location>
            <begin position="429"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="591" mass="62406" checksum="B3C5720E9E4D811F" modified="2014-01-22" version="1">MGRVPTPPTDSVPPTAHTAGPAQTRLELRTLRPELVQPYDAALPAAKAAVLGRLLGAFDREVLPGVTHRERRAGMLHVTLGDGRTLTAPAVQPFSPTEPGLAAHVSRPGGGGTFTEPALTAHLSQASGGGTFTEPDLLSQPSGDRTFVEPGLVPQPSGGGTFTEPGLVPQGGGIGTFDDPAALVRAIGLGPHGERFAAEVDNSVANLALAHADPHRESARSVLNRAAGDPDGLGRVEQLVLDGHPLHPCCRTRGGMTVADVLAYGPEHRPVIRLRKLRVPADRWYGDAAPVLYAHPWQAERLRARHPWLVDAGETGPVRPLMSLRTVAPTDGGGHVKTAVDVQMTSAVRTVSPAAVHNGPRLSVLLEKLTADLHLTVLRETDAGAVLVDGRPQRHLAYLRRQAPRVGPTETIVPAVAALLNNGGADPYRWWESLVRVLVPPLVTVLDRGVALEAHGQNTLVVLDGGRPVRIVYRDLGGVRVSTRRLLRHGVEAPPVEGDLPSDDPAVLRTKLAAAVLGTVCAELIAAMTRQGADAGRLWDSVAAALDSTGTPDAVHLLKEPLPVKATTAMRLAEDPLDDVWTFFDNPMAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Q4</accession>
    <name>U5W0Q4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363075.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22170"/>
    <dbReference type="EnsemblBacteria" id="AGZ42704">
        <property type="protein sequence ID" value="AGZ42704"/>
        <property type="gene ID" value="AFR_22170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4494"/>
    <dbReference type="eggNOG" id="ENOG5033NHJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067815_1_0_11"/>
    <dbReference type="OrthoDB" id="1742133at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="325" mass="35066" checksum="20CF2439C9BE78E0" modified="2014-01-22" version="1">MTDLLPPGDPELSPDRARVLKEAFLQEITRPGAARRRIPVLLPVAAAATVVLVTLVAGVALHQGSPATPAPGPAPVRVAAPVIALPPVHTDRVTSRADKIATGVTLPPSPTIRADQFVYVRSKVGFQQVVFGGGPAPSDGFTSASEGVVTQALETIRIREMWLPQKGDRGFVQDGKESFELDPISNSEHSAIPADPDQLLRKIYAETKGQGPGPDYTAFDWIGENMYETMLPPQVYVTLFRAAARIPGVVLIDDAVDGAGRHGVAVAYEDQGERREWIFDRRTLVYLGQRNYLVEDTPMGKAGTITGITAVLQRAVVDRQGQRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0R9</accession>
    <name>U5W0R9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363354.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22890"/>
    <dbReference type="EnsemblBacteria" id="AGZ42848">
        <property type="protein sequence ID" value="AGZ42848"/>
        <property type="gene ID" value="AFR_22890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4639"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_1_11"/>
    <dbReference type="OrthoDB" id="1054546at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="16"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="34826" checksum="BC9C0721C4A43D83" modified="2014-01-22" version="1">MHTRTLGSTGPVVSALGLGAMGMSGAYGEADRAESIATVHAALDAGVTLIDTGDFYAMGHNELLVAEALRGRSRDSYQLSVKFGMLRGPGPQFGGQDGRPEAVKNFLAYSLTRLGTDHIDIYRPARLDPAVPIEETVGAIKEMIDAGHVRHVGLSEVDAATIRRAHAVHPIADLQFEYSLISRAVETDILPTLRELGIGLTAYGVLSRGLISGHWSAGRVGGAGDVRGFSPRFASENVEHNLTLVDALRRVAEAKGCTVAQLAIAWVAAQGGDIVPLVGARTRERLSEALPALELDLTADDLAEIEKAVPAGSARGDRYPAAFMSSLGAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0W6</accession>
    <name>U5W0W6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative cysteine dioxygenase type I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16550"/>
    <dbReference type="EnsemblBacteria" id="AGZ41590">
        <property type="protein sequence ID" value="AGZ41590"/>
        <property type="gene ID" value="AFR_16550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3356"/>
    <dbReference type="eggNOG" id="COG5553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102185_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="18903" checksum="606CEA803A02011A" modified="2014-01-22" version="1">MTMAVGTALPALPGRVLSPRELQLWVAELATRTEVWEPLTGHDPARRHYASLYRDSDIDVWVLSWKVEDDTGWHDHDVSSGAVAVTRGAVTESNPRMGGDPVTRVVPAGRSFAFGPDHIHRMGGAVDGSVSIHAYSPPLWRMGQYSISPAGVLRRHAVSYADELRPLEAVADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W120</accession>
    <name>U5W120_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Uronate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_16860</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucuronate = D-fructuronate</text>
            <dbReference type="Rhea" id="RHEA:13049"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="ChEBI" id="CHEBI:59863"/>
            <dbReference type="EC" id="5.3.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; pentose and glucuronate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Uronate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.12" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361773.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16860"/>
    <dbReference type="EnsemblBacteria" id="AGZ41650">
        <property type="protein sequence ID" value="AGZ41650"/>
        <property type="gene ID" value="AFR_16860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3420"/>
    <dbReference type="eggNOG" id="COG1904">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044465_0_0_11"/>
    <dbReference type="OrthoDB" id="167900at2"/>
    <dbReference type="UniPathway" id="UPA00246"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008880">
        <property type="term" value="F:glucuronate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006064">
        <property type="term" value="P:glucuronate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003766">
        <property type="entry name" value="Uronate_isomerase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02614">
        <property type="entry name" value="UxaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="469" mass="52129" checksum="3FB41253C56B0253" modified="2014-01-22" version="1">MATLTVHPDRLLPADPGVRDIARRLYAAVRELPIISPHGHVDPRILLDDTPFADPASLLIQPDHYVTRLLHAGGVQLDRLGVGEGPLTEDKAREVWRLLCTNWHLLRGTPVRYWFDSELAEIFGITQRPDAGNADDLYDRIAGQLAGEGHRPRALLERFGIEVLATTDDPADDLEVHAALAADPGIKARITPTFRPDRYLEPAAPGWAEAVKRLGAVAETEVGDYAGYVAALERRRRYFVDHGAVSADHSHTDTITTPLDRAEAERIYRWALAGQVSEAEATAFRRHMILEMARMSCDDGLVMTLHPGVRRNHHPDTLVRYGPDTGADIPLAVEYTDALRPLLGRYGTHPGFHLVLFTVDPDVFAREVAPLAGFYPSVYAGAPWWFLDNPALIRRYQESVSEIAGFGRMSGFIDDTRAFCSIPARHDMSRRLDAGFLAGLVAAHRLDEDEALDTITMLVTEQPRKVFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W124</accession>
    <name>U5W124_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361666.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41591">
        <property type="protein sequence ID" value="AGZ41591"/>
        <property type="gene ID" value="AFR_16555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3357"/>
    <dbReference type="eggNOG" id="COG2159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_915012_0_0_11"/>
    <dbReference type="OrthoDB" id="1832218at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="3"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="32512" checksum="DEE389F16D48B4C1" modified="2014-01-22" version="1">MRIDCHQHLWPARFVDALRRRDSYPYLRDWTLHLAGEAPYSVVAADHDVDARRARELADGSGRVLVSLSSPLGIEYLPGDEGAELVALWHASALELGDPFGVWAAAQVLDPDPEGLAKVLREDRVAGLQLPATALADPAAIEHLAPLLQEAERADKPLLVHPGPAPADTADVPAWWPALVPYVSQQHRAWLAWHVAGRRQHPRLRIAFVALAGLAPLHHERLAARGGALGPLDPFVYYETSSYGTRAIDALVRVVGVDPIVHGSDRPYAQPCDPGLGDAFTRALFTTNPRHLLTGEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W181</accession>
    <name>U5W181_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mannitol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361775.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16865"/>
    <dbReference type="EnsemblBacteria" id="AGZ41651">
        <property type="protein sequence ID" value="AGZ41651"/>
        <property type="gene ID" value="AFR_16865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3421"/>
    <dbReference type="eggNOG" id="COG0246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027324_0_0_11"/>
    <dbReference type="OrthoDB" id="1442117at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019594">
        <property type="term" value="P:mannitol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000669">
        <property type="entry name" value="Mannitol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013118">
        <property type="entry name" value="Mannitol_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023027">
        <property type="entry name" value="Mannitol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013131">
        <property type="entry name" value="Mannitol_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01232">
        <property type="entry name" value="Mannitol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08125">
        <property type="entry name" value="Mannitol_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00084">
        <property type="entry name" value="MTLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00974">
        <property type="entry name" value="MANNITOL_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mannitol_dh" evidence="1">
        <location>
            <begin position="6"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Mannitol_dh_C" evidence="2">
        <location>
            <begin position="168"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="405" mass="43236" checksum="C2553D1F571E8F69" modified="2014-01-22" version="1">MRAPVRIVHLGLGGFFRAHQAWYTGAAPDAGRWGIAAFTGRSRGLADDLARQDGRYTLIVRGPSHDEMAVQEAVVKALPGTDTAAWSAHLALPEVAVVTLTVTESAYAEGGVVARRLVDGLAARRAAGAGPLAVVSCDNLPRNGSRTADVVTALAASTDPALAPWIRDSVSFVDTMVDRITPRTRPEDVEVVLAETGWADAVPVVTEPFTEWVLSGDFPAGRPAWDARFVDDVHPYETRKLLLLNGGHSLLAYAGSARGHETIAAAVADPVCRGWLDDWWDEAARYVPLPAGDVLAYRKALLERFANPRIRHTLAQIAADGSQKVPIRALPVVRGERAAGRVPVGGVRILAAWIDHLRGIGAPVKDAGAAAYRERAGSARDVLTLLAPDLATDEVLLAEIRKALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1D8</accession>
    <name>U5W1D8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_22810</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22810"/>
    <dbReference type="EnsemblBacteria" id="AGZ42832">
        <property type="protein sequence ID" value="AGZ42832"/>
        <property type="gene ID" value="AFR_22810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4623"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="121"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="121"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="23076" checksum="C27F1BD09DF2F774" modified="2014-01-22" version="1">MVDDQPTVVDMLASVLTFHGFAVDTARTGEQAVEKAAEHSPDLVLLDTMLPDGDGLDVCRRLRAGGSAMGIMFLTAPDASADLIAGLAYGDEDWITKPFDVEVLLTRVRALLSRSGSPSGDRLLRYADVELDQDTHEARRGGEVVPLSRTELEVLRYFLQNSGRVLSRGQIEAATASPDVDADVTSLRTELDRLGAPLIVTHRGFGYALRAGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2A1</accession>
    <name>U5W2A1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24965</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363849.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24965"/>
    <dbReference type="EnsemblBacteria" id="AGZ43259">
        <property type="protein sequence ID" value="AGZ43259"/>
        <property type="gene ID" value="AFR_24965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5057"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_4_1_11"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="3"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="152"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="29733" checksum="8F75AEDF704286A9" modified="2014-01-22" version="1">MTAPVIVGVDGGESGADALVLARWTAQTLDAPLVVAVVHPAPSALGSGRVDAEWIADRHRAAEAVLDGARTVLAGATGEVTYRVVASTSAAHGLHDLAEELKAEVIVVGSGRAGPRHRLFAGSTAERLLAGSVCPVAVAPAAMETPPGAVGRIGVAYVDTPDGRAALDMAARLAARTGSPLRLYTVVAEADATLPFLVGQDAEHAFLETARETYELSLSRAAASVDVEADWEVRAGDVLESLADLDEVDVLFCGSRGYGPARRVLLGGVSARLVRRARRPVVVVPRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2C2</accession>
    <name>U5W2C2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43388">
        <property type="protein sequence ID" value="AGZ43388"/>
        <property type="gene ID" value="AFR_25620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5193"/>
    <dbReference type="HOGENOM" id="CLU_2696157_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="73" mass="8159" checksum="F7A1282699829EBB" modified="2014-01-22" version="1">MSLHFLPRSAAMLRADDLTVVHHDDTVSHFTDVIYTLTRDGLRILTLTGDEKIIMAHDILTTHAHFAHTPLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2E0</accession>
    <name>U5W2E0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362439.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18960"/>
    <dbReference type="EnsemblBacteria" id="AGZ42066">
        <property type="protein sequence ID" value="AGZ42066"/>
        <property type="gene ID" value="AFR_18960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3851"/>
    <dbReference type="eggNOG" id="ENOG5032R61">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1407364_0_0_11"/>
    <dbReference type="OrthoDB" id="1739497at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="184" mass="20312" checksum="7B10239CA9273A9C" modified="2014-01-22" version="1">MDVPELLRQAAREIPAGLPVADVDDYLAANEWEIALDVLLDLGDTYPATTSYWQTLGDAAHLMHLERNAAWCAWRRREVLHGIIRAELQLLPGSRTTPIPGDGKLRPLWDIGLVTPAGLPDLAVALIWVEYAPDLPPDGRGSVRLAPLTAARWHHLQPGDRITMHEDRPIAGTAFITELAFPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2E4</accession>
    <name>U5W2E4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerophosphoryl diester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363894.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25190"/>
    <dbReference type="EnsemblBacteria" id="AGZ43304">
        <property type="protein sequence ID" value="AGZ43304"/>
        <property type="gene ID" value="AFR_25190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5104"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_3_6_11"/>
    <dbReference type="OrthoDB" id="1542724at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="15"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="27842" checksum="0E12AD7F22C39DBF" modified="2014-01-22" version="1">MSVGLRFPYLDSPAPLAFAHRGGAAEGDENTAEAFGRAVALGYRYVETDVHATADGVPVVFHDRTLERLTGTEGTIAALRWSDLATLRVGGAGAVPRLDDVLAAWPQIRFNIDVKSEAAVAPTVEAIHHAGASGRVLLASFSDARLTRLRRLAGPSVATSLGRGEVARLRISSLTGTRLRLPPSAVAAQVPVRHGRLTIVDRRFLSRTHRLGLQVHVWTIDDPAEMHALLDLGVDGIMTDRTQTLRDVYTARGLWPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2L2</accession>
    <name>U5W2L2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_19370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362518.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19370"/>
    <dbReference type="EnsemblBacteria" id="AGZ42146">
        <property type="protein sequence ID" value="AGZ42146"/>
        <property type="gene ID" value="AFR_19370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3932"/>
    <dbReference type="eggNOG" id="COG2018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139202_1_0_11"/>
    <dbReference type="OrthoDB" id="1963330at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="123" mass="13329" checksum="F4D5D1448201BB66" modified="2014-01-22" version="1">MPDMDVALKDAMTIDGAIGVALVDHTSGMALGVAGGTRDFDLTVAAAGNTDVVRAKLRAMDLLGLDEQIDDILITLETMYHLIRPLKARSGHGLFLYLSLDRSRANLAMARHQLRRIESALDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2R8</accession>
    <name>U5W2R8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mannitol-1-phosphate/altronate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25800"/>
    <dbReference type="EnsemblBacteria" id="AGZ43424">
        <property type="protein sequence ID" value="AGZ43424"/>
        <property type="gene ID" value="AFR_25800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5230"/>
    <dbReference type="eggNOG" id="COG0246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027324_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019594">
        <property type="term" value="P:mannitol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000669">
        <property type="entry name" value="Mannitol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013118">
        <property type="entry name" value="Mannitol_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023027">
        <property type="entry name" value="Mannitol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013131">
        <property type="entry name" value="Mannitol_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01232">
        <property type="entry name" value="Mannitol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08125">
        <property type="entry name" value="Mannitol_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00084">
        <property type="entry name" value="MTLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00974">
        <property type="entry name" value="MANNITOL_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mannitol_dh" evidence="1">
        <location>
            <begin position="29"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Mannitol_dh_C" evidence="2">
        <location>
            <begin position="221"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="492" mass="54380" checksum="9897059BAB720F4D" modified="2014-01-22" version="1">MLPLNARNLSSLPGELPVPAYDRGALRTGIVHFGVGGFHRAHEAMYLDRLMNDGKALDWAICGVGVMPADRRMRDALDGQDGLYTLVVKAPDGTLEPRVIGSIKEYLFAPDDPEAVIEKMASADVRIVSLTVTEGGYNFNAVTGEFVADNPDVQHDLQPGNPPKTTFGLITAALARRRERGSAPFTIMSCDNIQGNGHAARRSFVAYANLVDPELGAWVEEHVAFPNSMVDRITPVTTDDDREEVRKRFGVDDAWPVVCEPFTQWALEDAFPLGRPPFEDAGVQVVADVEPYELMKLRLLNASHQALCYFGYLSGFRLVHDVTQDSLFADFLLAYMDREATPTLEPVPGIDLDEYKHQLIDRFSNAQVKDTVARLCAESSDRIPKWLLPVIRHNLETGGEILRATAVVASWARYAEGVDEQGQKIEVVDRLRDTLVATAQRQAEDPLAFIANRDLFGDLIDNERFVSTYRSVLASLHSKGARATLEELATAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2S7</accession>
    <name>U5W2S7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5-amino-6-(5-phosphoribosylamino)uracil reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19720"/>
    <dbReference type="EnsemblBacteria" id="AGZ42216">
        <property type="protein sequence ID" value="AGZ42216"/>
        <property type="gene ID" value="AFR_19720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4001"/>
    <dbReference type="eggNOG" id="COG1985">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036590_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23863" checksum="D020DD1CDF79EA31" modified="2014-01-22" version="1">MSIDGYVDTPDDERLLLSNDADFDRVDEVRAGCDAILVGAATVRQDNPRLLVRDETRRKARVERGEQPDPVKVTVTNSCDLDPDARFFTLGEADKLVYCVSSALPNARHKLGRVATVIDGGDPLDLGQVTADLHTRGIRRLMVEGGGMIHTGFLTAGLADELQLVVAPFFVGDSRAPRFVGDGTFPWGPRRRADLTEVRQIGDVVLLRYALSDRYRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Z9</accession>
    <name>U5W2Z9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Putative cytochrome oxidase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_20145</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome ubiquinol oxidase subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362673.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20145"/>
    <dbReference type="EnsemblBacteria" id="AGZ42301">
        <property type="protein sequence ID" value="AGZ42301"/>
        <property type="gene ID" value="AFR_20145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4086"/>
    <dbReference type="eggNOG" id="COG1271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030555_3_3_11"/>
    <dbReference type="OrthoDB" id="271551at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070069">
        <property type="term" value="C:cytochrome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002585">
        <property type="entry name" value="Cyt-d_ubiquinol_oxidase_su_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365">
        <property type="entry name" value="PTHR30365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01654">
        <property type="entry name" value="Cyt_bd_oxida_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006446">
        <property type="entry name" value="Cyt_quinol_oxidase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 12" id="KW-0349">Heme</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ42301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="463" mass="50184" checksum="EF2E10ED2D6EEF61" modified="2014-01-22" version="1">MDVLDLTRLQFAVVTIYHYFFVPLSISLAGAAAGLQLTWMRTGQEKYLHLTKFVGKLLIVTFAVGVVTGLVQEFQFGLGWSNFARFYGDVFGPTLAIEGMLAFFLEATFLALWYFGWDRLPRKLHAATIVIVAVGTVLSAFIILAANSFMQNPVAYSLDPVTGRARLDSFGELLLNKVNLAAFPHTLAGACMVGGSLLLGIAIWRRRSDAGFAPLARLGAWMTVTGGALTALTGDHLGKVITEVQPMKMAAAESLYETTTGAPFSIFAIGDISGGKPFFSIELPWLLSILAKGSPGAEVQGIDDLQAQYAAQFGPGSYVPMIPVAFWTFRLMIGIGMLAMAAAALYLWTTRKGRELDESRRWSRLFLRFLPLIPLLPTAANSLGWIFTETARQPWLAFGISRVADGISPGLTSGEVLASLLAFTAVYGIVAVVWLRLVRHLSRQPLAPPPVEAAAEQTAVPVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W322</accession>
    <name>U5W322_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF695 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362698.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20270"/>
    <dbReference type="EnsemblBacteria" id="AGZ42326">
        <property type="protein sequence ID" value="AGZ42326"/>
        <property type="gene ID" value="AFR_20270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4110"/>
    <dbReference type="eggNOG" id="ENOG5030BCD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_801252_0_0_11"/>
    <dbReference type="OrthoDB" id="976735at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016097">
        <property type="entry name" value="DUF695"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05117">
        <property type="entry name" value="DUF695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF695" evidence="1">
        <location>
            <begin position="213"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="36018" checksum="E850C1F9E880FB6A" modified="2014-01-22" version="1">MIFKRRAKGDPATAVTAFWTWWATGRSRVEQAIAGGSWGPVLIDEINALVTAIDPGLAWEFGAGTASQHLLVVTANGDRALRAAAERWRRAGPAPDTAFGYAASRQANPSALDSRLDLDGHRFELGALRFAADVDDDAVQVDVCVWHPAFPDLPDTTRVQIAFLALDWLLGEDGVEIWVREIDTTGEEGPDLTGAELAATVAALMPAGGEPQWTVLSGNRGGRPVLASVCVPLKAARWPAADTHVRLDVPFRSANAGGLPVDGSLAALHAFEDRLFAYADGPVLVAHESADGVRTYHLYADRQAAVDALAPITASWPEGRVRTTVTPDPAWKGVSHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3J6</accession>
    <name>U5W3J6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP_transf_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560037.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27190"/>
    <dbReference type="EnsemblBacteria" id="AGZ43699">
        <property type="protein sequence ID" value="AGZ43699"/>
        <property type="gene ID" value="AFR_27190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5510"/>
    <dbReference type="eggNOG" id="COG2068">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061980_2_2_11"/>
    <dbReference type="OrthoDB" id="1432587at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="5"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="20500" checksum="AA62C4799C05102E" modified="2014-01-22" version="1">MFVTGVVLAAGNSVRFGLGEAKQLLPYRGRTLLDAGLDLARACAFDQRLVTLGGSAAAVRARADLTGFDTVDVPAAGTGCGASVSTAVRSVDPRADGLVLLLGDQPGVRVATVTALLTAGGPLRVSRYADSLGHPFWFGREMFGALGELHGDKAVWKLLHSGTHPVTEVEVAGPVPIDVDTREDYERLLAGDHLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3J9</accession>
    <name>U5W3J9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_27215</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27215"/>
    <dbReference type="EnsemblBacteria" id="AGZ43704">
        <property type="protein sequence ID" value="AGZ43704"/>
        <property type="gene ID" value="AFR_27215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5515"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="146"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="23318" checksum="663CA7A5B219D3BF" modified="2014-01-22" version="1">MVLVDDQSLIRAGIAVLLAGGDDGAIEVVGEASDGGGVVALVRRTRPDVVLMDIRMPGTGGLRATRELRADPDCGGVAVLMLTTFDTDDEVLGALRAGADGYLLKDAAPDALRAAVRAAAAGQAVLAPGITRQVMAHAATAVPARPDPRLALLTTREREVLIGLARGDDNPAIARAMRLSPETVRTYVSRILSKLGATSRAQLVALAHRSGLAATEAGADVDSGMPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W460</accession>
    <name>U5W460_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560256.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28290"/>
    <dbReference type="EnsemblBacteria" id="AGZ43919">
        <property type="protein sequence ID" value="AGZ43919"/>
        <property type="gene ID" value="AFR_28290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5735"/>
    <dbReference type="eggNOG" id="COG0308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_527634_0_0_11"/>
    <dbReference type="OrthoDB" id="1514411at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="461" mass="50257" checksum="EB36132A76686741" modified="2014-01-22" version="1">MIETPHRSRTGLFIGLAAAALVVVVGAGVAVGVVVGGKDDGPAATGPKTAVLGQDSTSVRVDKRELDTLANDRSTALVKGDLDDFLAPLDPAATTMITKERQLFANLKKIKFARAQYAPFDQVGRTADRFGRAVTVNLDVAFVHQIDGFDTDPVEEWYRWTVVKKDAKAPLVITAIGPAPADAVSNSESVMYPAPWDKWPDMQVVKTEHTLFLVDKPMLGKTRKYAPVAETAARRVLADWKASGVDAPVFDRFVSSLVPTRAQLGSLYQVIKGETTEAGRMMPMSTYGSDRRVGGTRSLFDAGSGFFSGGDTAEIFRHEYAHAAVEALNANDNPDAGIWGPERWIIEGFAEYVANRGRPTVGIRGPSAAKLVRSDFKGTLPAAVAWDVPDSAVMNYHYYLGHQAFRFMAKKYGSNKVFEFTATYYRGGQSLDEVFQKVLGTSRSAFESQWASFVRTDARNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W484</accession>
    <name>U5W484_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28810"/>
    <dbReference type="EnsemblBacteria" id="AGZ44023">
        <property type="protein sequence ID" value="AGZ44023"/>
        <property type="gene ID" value="AFR_28810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5840"/>
    <dbReference type="eggNOG" id="ENOG5030RFJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087604_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="24319" checksum="184D997D2859F85D" modified="2014-01-22" version="1">MPVVTSMFKQWKKQWAIGRVKAGDGHPLKPFRWWQTFTGRKLFSLRVQQDGQTIEYAVEVRQGSERTMAHVFLNGRHHAQAPLPAVIPVAHGTIEIAKSNFGLKRASFVSDDNVEHQLVADPSSLIGRRLRYEREHPQASRIIGAVSIAVLLVGVGLNLIQLLEPLSAIPPVQERFGTFESPVSLPVWLNVALGAAAVLGSMERGLRLKYSWLLDGMAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4W1</accession>
    <name>U5W4W1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sialidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560496.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29490"/>
    <dbReference type="EnsemblBacteria" id="AGZ44159">
        <property type="protein sequence ID" value="AGZ44159"/>
        <property type="gene ID" value="AFR_29490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5977"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_519386_0_0_11"/>
    <dbReference type="OrthoDB" id="1691648at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666080" evidence="1">
        <location>
            <begin position="32"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="18"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="524" mass="54206" checksum="3CB612CA79187833" modified="2014-01-22" version="1">MRIRTAASACAVALVAAAALVAGNLHGNAFAADSLSAGRSATASSATAGNTAAKAVDGDSGTRWESAQGLDPQWLQVDLGSAQALGRVELDWERAAAKSYSLQLSDDGQTWRTVYSTTTSAGGKQSVPVSGTGRFVRMYGTARTTQYGYSLYEFGVYAPDGTGGQPSPSGAAAISGVSLIDNTARQPVAGFNPLRDGATVDLAQLAHRTLSLRADPSSGAAVGSVFFTLTGAGGNTYTRVESKAPYFLCNDFIDCPQLLVAGTYTVTVQAYTGADRQGAVLGAPLTVHFTVRGDAAPTDALDVLFIGNSLIGTRTGATNQDTADVVKSLAATSGRALNLTEVIHFGNTLQETWDAGEVKSALSSAKKYDLILLQEQSTLVTKNFAAAEKALLDTYAPAVVKNLEPGGRVLLFENWALADNAGFPSRAANVAAIESGYARLSAELTVPNTIVPISDAFEKIIAADGTSGLIVADGRHPNDKAIYLDAAVLYGILFTTSPENLAGLYLPAATAARLRAVAADVLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4W4</accession>
    <name>U5W4W4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29515"/>
    <dbReference type="EnsemblBacteria" id="AGZ44164">
        <property type="protein sequence ID" value="AGZ44164"/>
        <property type="gene ID" value="AFR_29515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5983"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976782_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="28"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="13683" checksum="35575795EFB945BC" modified="2014-01-22" version="1">MVGPGSFAGLPSRQVQRLGRESAAVQVRIAAKARPIGPHQIRDLTASTLLDAGYGVHEVAERLGHDPATLMRYYTRVDATRRLQATDRITDLMTVSEVPPRPLRNWGGCTASLLFDDGKSVETLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Z4</accession>
    <name>U5W4Z4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH/Ubiquinone/plastoquinone (Complex I)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_30140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560625.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30140"/>
    <dbReference type="EnsemblBacteria" id="AGZ44288">
        <property type="protein sequence ID" value="AGZ44288"/>
        <property type="gene ID" value="AFR_30140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6105"/>
    <dbReference type="eggNOG" id="COG0651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_9_2_11"/>
    <dbReference type="OrthoDB" id="1887859at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="3">
        <location>
            <begin position="130"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="501" mass="52144" checksum="9AFDF13177A1690F" modified="2014-01-22" version="1">MTFLVPLPVVMPLIGAALTLMMAGKPRAQRTVSLTVLTGTLLVSIALLVLSTMDGPLVVAVGGWVAPLGIVLVADQLAALMLVVSSAVTVCVLVYSIGQGMADGNEETPLSIYHPTYLILTAGVTNAFLAGDLFNLYVGFEILLVASYVLLTLGGTENRIRAGTTYVVVSLVSSLIFLTAIGLVYAATGTLNLAQLVGRLDALPDSLRLMLQGMLLLAFGIKAAVFPLSAWLPDSYPTAPAPVTAVFAGLLTKIGVYAIIRTETLLFPGGRAANLLMVAALLTMVIGILGAVAQSDIKRLLSFTLISHIGYLLFGVGLSTRLGLASAIFYVVHHITIQTTLFLTAGLIERRGGSTGLERLGGLARLSPLLAGLFFVPALNLAGIPPFSGFIGKIGLLQAGADDGGPLAWSLVAGGVLTSLLTLYAIARVWNLAFWRGPNPALPEASRALLPRLMVAPTLALVVLGVGLTVVAGPLFDISDQAAGYLLERSGYVHAVFPEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W556</accession>
    <name>U5W556_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24185"/>
    <dbReference type="EnsemblBacteria" id="AGZ43105">
        <property type="protein sequence ID" value="AGZ43105"/>
        <property type="gene ID" value="AFR_24185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4899"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009318_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019405">
        <property type="entry name" value="Lactonase_7-beta_prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011964">
        <property type="entry name" value="YVTN_b-propeller_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10282">
        <property type="entry name" value="Lactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="SSF50969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02276">
        <property type="entry name" value="beta_rpt_yvtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666361" evidence="1">
        <location>
            <begin position="23"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="354" mass="37001" checksum="AFDBAD9231653EB5" modified="2014-01-22" version="1">MRGVTQAGLGVLALVVSAGVGAATVVVTRPPAGPVTIAAAPVSAVPSATSTRRSSIPGPRVVATLRVAAEPGGVAVSPDSRTVYVAGQSSRFVSVIDAATRSVTDVRVPRPPRFVTVARDNSRAYVAMYEDDGSHSGIAVIDAETREVTKVVATGPQPYDLAVAPDGRVWVPIHSAGRVEVYTGDELRRVVTVPVAPNPHSVRFGPEGLRAYTPAHESSVVSVIDMWDDSVRQSVPVSKAPHSLAVSPDGRTILVACYDADRVDRIDSLTLRRSGSFPVGDKPQSVTFAADGRHAYVADEDDNTLTVLNVRTGATTAKVRVGRSPRAVAVAPDGLYAYVSAGDDNTVTILRVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5F8</accession>
    <name>U5W5F8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560715.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30595"/>
    <dbReference type="EnsemblBacteria" id="AGZ44379">
        <property type="protein sequence ID" value="AGZ44379"/>
        <property type="gene ID" value="AFR_30595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6194"/>
    <dbReference type="eggNOG" id="COG0099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151796_1_0_11"/>
    <dbReference type="OrthoDB" id="1950120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="105" mass="11326" checksum="C3CD4405A0E2C26F" modified="2014-01-22" version="1">MPLPSLSPEQRAAALEKAAEIRKARAELKDQLKQGKTTLAAVLDRAEGDDVVGKLKVSAVLQALPGIGKIRATQIMEKLKIADSRRLRGLGEQQRKALLGEFAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5K9</accession>
    <name>U5W5K9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560764.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30845"/>
    <dbReference type="EnsemblBacteria" id="AGZ44429">
        <property type="protein sequence ID" value="AGZ44429"/>
        <property type="gene ID" value="AFR_30845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6244"/>
    <dbReference type="HOGENOM" id="CLU_2056313_0_0_11"/>
    <dbReference type="OrthoDB" id="2200755at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="12899" checksum="2C5BD7D18B7066DA" modified="2014-01-22" version="1">MSASELRDQTLGRQRELAARHEVLNRLEPGPDRYDDQLARVFDATNDLVAVLDRAASRTWRRRMTVSFVLLGLVVVVAGLVAAGLLPEYGLIAALGLLVAAVAVWASTRTLAGADSVETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5S9</accession>
    <name>U5W5S9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oxidoreductase FAD-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_25125</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363881.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25125"/>
    <dbReference type="EnsemblBacteria" id="AGZ43291">
        <property type="protein sequence ID" value="AGZ43291"/>
        <property type="gene ID" value="AFR_25125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5091"/>
    <dbReference type="eggNOG" id="COG1018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_14_2_11"/>
    <dbReference type="OrthoDB" id="1885467at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="37"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="5">
        <location>
            <begin position="267"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="37888" checksum="B28C745EE6FA1AFA" modified="2014-01-22" version="1">MAVTQTLRSGAWRVVELITTPLVPADYLDVVAPLRNASVLRAKIESVRPETRNSVTLVLRPGRGWQPHEPGQYIRIGVDVDGVRLWRAYSVTCAPGRLSVTVNAVRDGVVSNYLIRNAKRGMIVHLDNPAGEFTLPTPRPEKVLFVTAGSGITPVMGMLRSALHELSDVVVLHSAPEREAVVFGEELRTLAADGRIRLIERHTRTDGRISTTELAELVPDLFERHTWACGPNEMLDDLGAHWALAGAAERLHVERFRPTIIAAGEGGKVTFGRSGTVVDAEGGVPILEAGENAGVLMPSGCRMGICFSCVLPMTEGSVRDLRDGSITTAVEGDRIPVQTCISAAAGPCQLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Y2</accession>
    <name>U5W5Y2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_30810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30810"/>
    <dbReference type="EnsemblBacteria" id="AGZ44422">
        <property type="protein sequence ID" value="AGZ44422"/>
        <property type="gene ID" value="AFR_30810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6237"/>
    <dbReference type="eggNOG" id="ENOG5032MRU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217694_0_0_11"/>
    <dbReference type="OrthoDB" id="2150659at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666378" evidence="2">
        <location>
            <begin position="31"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="23229" checksum="EB318C2FFC022C23" modified="2014-01-22" version="1">MAVAFLAVLVAVGSLGVAGWAVHRANVAVAASERLTVAAPAAGQPTPEPPAVESAAPAPSPTTEASTETSTGEESGGEAPTLSPTAVFTVQYEKRKLSVQMPANRCSSRSIDLDEPQLDVGGYTNDLEVKGTCSGTMTLNFGQGVSGAADAEDLSTTPKACNERIQLSALPRDEPLPLRKGQVLCVRTSSAQAKTLGISQKMTLVNVTGVGATGLVTLEVTAWNVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6T0</accession>
    <name>U5W6T0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561044.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32270"/>
    <dbReference type="EnsemblBacteria" id="AGZ44712">
        <property type="protein sequence ID" value="AGZ44712"/>
        <property type="gene ID" value="AFR_32270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6532"/>
    <dbReference type="eggNOG" id="COG2856">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054419_3_1_11"/>
    <dbReference type="OrthoDB" id="1812319at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="17462" checksum="70B709387AE68610" modified="2014-01-22" version="1">MTPLQFRRGDATSPQAKGPKVIAHICNDLGGWGKGFVLAVSRRWPEPERDYRQWHRERSKNDFRLGAVRLIQVRPDTWVANMIGQHGMKHGSSGPPIRYDAVEKCLTTLGEQAAGLEASVHMPRIGCGLAGGKWERIEPLVQSALSDRGIPVTVYDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6V7</accession>
    <name>U5W6V7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561070.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32395"/>
    <dbReference type="EnsemblBacteria" id="AGZ44737">
        <property type="protein sequence ID" value="AGZ44737"/>
        <property type="gene ID" value="AFR_32395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6556"/>
    <dbReference type="eggNOG" id="ENOG5032ZBB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166645_0_0_11"/>
    <dbReference type="OrthoDB" id="1917445at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045423">
        <property type="entry name" value="DUF6510"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20120">
        <property type="entry name" value="DUF6510"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="88" mass="9309" checksum="5A0224BEE453CD47" modified="2014-01-22" version="1">MTDQLDGNAMAGDLREIFAIDLTSAVATCAGCKEPSVIATLRIWGPAPGLVARCPSCDDVVVRLVRGPEEAWLDLRGASSLRIPMPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6V9</accession>
    <name>U5W6V9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chemotaxis protein CheR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560058.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27295"/>
    <dbReference type="EnsemblBacteria" id="AGZ43720">
        <property type="protein sequence ID" value="AGZ43720"/>
        <property type="gene ID" value="AFR_27295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5531"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_985636_0_0_11"/>
    <dbReference type="OrthoDB" id="1414192at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="49"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="30386" checksum="53093A320E9CAD6E" modified="2014-01-22" version="1">MTTDRWADWLLRHRDGGSAELRDLFAPQLLAFRDGVLGRAAVAPGDTLLDVGTGTGLIGLGALDLVGPSGHVIFSDVSPALLDECRRHADPERSSFVEAGADDLSAIPDASADVVTTRSVLIYVTRKAEAFAEFRRVLRPGGRLSIFEPINSFPVRREVHDMFGLGPTPVADLVSKVRQVYTGHAAMVDFDERDLLGWAVEAGFTQVTLDYRAEVDVPGPPIGDWAALKRTAPNPLAPTYGEAIEQALTPAERDRLDACVESLAGLPTRHTMATAYLSAVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Y9</accession>
    <name>U5W6Y9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bilirubin oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33550</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561305.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33550"/>
    <dbReference type="EnsemblBacteria" id="AGZ44968">
        <property type="protein sequence ID" value="AGZ44968"/>
        <property type="gene ID" value="AFR_33550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6790"/>
    <dbReference type="eggNOG" id="COG2132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009100_2_2_11"/>
    <dbReference type="OrthoDB" id="971126at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="PTHR11709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="82"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="3">
        <location>
            <begin position="398"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="507" mass="55622" checksum="F9DD4EB006196965" modified="2014-01-22" version="1">MTGPLSRRGLLTLAGVAGLAGLAGCGRPVDGPGTGSLLTSSLPLPERFRTPLPIPPVARPVLTNSAGDHIVLTQRVADAHLLPGVTTQIWGYDGIFPGPIIRARRGRPLTVTMRNQLPVPTSTHLHGGIVPADSDGFPLDLITPAGYSPAPPVQHGTGARVHEVSRDYRYPMDQRAGLLWYHDHRMDFTAPQVWRGLAGMMIVSDDEEDALPLPRGDRELPLLICDRAFEADGSLRYPLRDMAMHHPGADGDFLSGIFGDVILVNGAAWPRHEVANVRYRLRLVNGSNARRYRLGLSPGTDLIQIGTDDGLLAAPVMHREIVLSPGERCDVLVDFSAYPIGSEVTLTNTLGTGPAAEIMRFAVTRSATDDTRVPPKLADVPVLERSEAVEVRQFDFRFGAGRWQINNQPYDPAGSVAAPRLGTVELWRFTSDFHHPVHVHLGHFQVLSRNGRDPDPWEGGWKDTVDVRPYEAVEVLVKFAGYRGRYMLHCHNLEHEDMAMMANFDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W798</accession>
    <name>U5W798_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative LacI-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33805</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33805"/>
    <dbReference type="EnsemblBacteria" id="AGZ45019">
        <property type="protein sequence ID" value="AGZ45019"/>
        <property type="gene ID" value="AFR_33805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6843"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="335" mass="35991" checksum="85BF94284B99A99A" modified="2014-01-22" version="1">MDQVAEAAGVSRATVSRVINGAPSVDAKIREIVQRAIADTGYVPNVAARSLVTRKSNSVALVISEPDRPYDSSFLNRIFTDPYFGRVTAGATGALRPHDIHLVIIPTDSTDHHHVLRYLRQGHVDGVLLISSHEQDPLPRQVHELGIPAVVSARPASPLAASFVDVDQRLGARLAAEHLLARGCRRLATISGPLDIPSGQDRLDGFRSYLANRGMGDVPAVEGDFTRNGGEEGARQLLGLYPDIDGLFVASDLMSEGALRAVQDLGRRVPDDIAVVGFDDSSAALDCRPLLTTIRQPVEEMAAEMAEVLMAHIAAPGRLPRSVTFQPTLVIRESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7G0</accession>
    <name>U5W7G0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28370"/>
    <dbReference type="EnsemblBacteria" id="AGZ43935">
        <property type="protein sequence ID" value="AGZ43935"/>
        <property type="gene ID" value="AFR_28370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5752"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="88" mass="9255" checksum="5319C747F165A70D" modified="2014-01-22" version="1">MSVHGELVMEEAPRLRNLLTALLNRGDVTAVELDLADVTFIDASGAGTLIVAHRIAANLRVDLRLSAVSAPTAQVLILMAAADLIPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W840</accession>
    <name>U5W840_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Threonine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_29125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29125"/>
    <dbReference type="EnsemblBacteria" id="AGZ44086">
        <property type="protein sequence ID" value="AGZ44086"/>
        <property type="gene ID" value="AFR_29125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5900"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="9"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="35683" checksum="F7DA299F877A5994" modified="2014-01-22" version="1">MTKGAAYEGAGQLRTVSVQVEPPGPGELQIAVAYCGLCGTDLHIAHGNMDARVRTPLVFGHEASGTVAAVGPGVSDWEVGDPLTVMPLIWDGTCAACLAGHQHICQNLTFVGIDSPGALQQRWNVPASIAVRLPAELDLRTAALVEPVAVAVHDVRRSGLQVGDRAVVLGGGPIGVLIACVARQAGADVLIAEIDESRRDRIAGLGFTVSDPAGTDLGAEVERWTGGVGADVVFEVSGAAAAVRSATGLARVRGTIVIVAIHAAPREVDLQRLFWRELRVLGARVYQRSDFERAISLLSLGAIPVSELITDVVPLDRTGDAIADLSAGRAMKILVDVAGQDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W879</accession>
    <name>U5W879_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44126">
        <property type="protein sequence ID" value="AGZ44126"/>
        <property type="gene ID" value="AFR_29325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5942"/>
    <dbReference type="eggNOG" id="COG5485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100997_5_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07366">
        <property type="entry name" value="SnoaL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="13718" checksum="0B3CE3BB2B25F2D9" modified="2014-01-22" version="1">MDQFTHDEINFNEQMITRAEYCAVIQGNIDAVPDFFWWVDELVIDGNLLIARLVDHGTPAKPWLGLEPTGRQINFIEYAIYRFQDGRFNWINVVLDRPSIARQLSGVPEQVYAAQQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8F1</accession>
    <name>U5W8F1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560538.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29700"/>
    <dbReference type="EnsemblBacteria" id="AGZ44201">
        <property type="protein sequence ID" value="AGZ44201"/>
        <property type="gene ID" value="AFR_29700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29700"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727436_0_0_11"/>
    <dbReference type="OrthoDB" id="2014094at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="22"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16544" checksum="3C70F78D3952119B" modified="2014-01-22" version="1">MHTPKPHHEFFKVSRLVPEDQNLATVDTQTKVRDALTLMAGSGFDQVPVTHGSRVVGVFSHRSLCAGLNAVRRTDDPQDHLVVDFLEDLSFVRTGTDFGDVLRAMEKTDVALLGDEENLSLSSPAATLRGFSGTPHDRSSSCRTSSWPLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8G8</accession>
    <name>U5W8G8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29800"/>
    <dbReference type="EnsemblBacteria" id="AGZ44221">
        <property type="protein sequence ID" value="AGZ44221"/>
        <property type="gene ID" value="AFR_29800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6038"/>
    <dbReference type="HOGENOM" id="CLU_1302735_0_0_11"/>
    <dbReference type="OrthoDB" id="2177833at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="22508" checksum="468A0B4087AABE49" modified="2014-01-22" version="1">MFPISTTPSPSGSDRLRFLGLPKFAGFIAIVSAVIGLLAYIGDVQERWQPDDVAAAPASPVQSRSATPSASPSTAAPGPSPATKPKQRNGAFQLTAQPLDARTVEVAVTPPTGLAAGRDYWFFVEIDWRDGNTDYYPREKLTGATQTLVIDIPPDATQQADRTGRVYALTTDQSREAAVRLQRQRTSKEDDFFADAPGATASQATGLPFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8S6</accession>
    <name>U5W8S6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_30675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560730.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30675"/>
    <dbReference type="EnsemblBacteria" id="AGZ44395">
        <property type="protein sequence ID" value="AGZ44395"/>
        <property type="gene ID" value="AFR_30675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6211"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_3_2_11"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="5"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="233" mass="24835" checksum="4A03C706460098E0" modified="2014-01-22" version="1">MTTYVLVPGLWIGAWAWREVTAALRTAGHDVFPLTLTGVADRAHLLGPDTDLDLHTEDIVRLVETEDLHDVVLVGHSYGGMPVSAAALRLKDRVSRVIYVDSGPLPEGTTQASMDPGDPPAGDTVPAREWDADKDPVLLAGLDDAALTLLRERSTPHPAASVVQPLGARATSADLPTTLVACTFPIEQIREMMAAGHPFFAGLGDAEIVPLATGHWPMLSEPVALAALLEQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8V4</accession>
    <name>U5W8V4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="7" type="ORF">AFR_30580</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30580"/>
    <dbReference type="EnsemblBacteria" id="AGZ44376">
        <property type="protein sequence ID" value="AGZ44376"/>
        <property type="gene ID" value="AFR_30580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6191"/>
    <dbReference type="eggNOG" id="COG0452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_0_3_11"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00353"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 4" id="KW-0288">FMN</keyword>
    <keyword evidence="3 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="5">
        <location>
            <begin position="5"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="DFP" evidence="6">
        <location>
            <begin position="186"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="191"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="409" mass="42223" checksum="BE80676E00735DBB" modified="2014-01-22" version="1">MLMPEVVLGVCGGIAAYKACELLRLFTESGHGVRVVPTASALKFVGAPTWAALSGRPVATEVWDDAHEVPHVRIGRHADLVVVAPATADMLAKAAHGIADDLLTNTLLTATCPVVFAPAMHTEMWENPATQANVATLRSRGAIVIEPAVGRLTGADTGKGRLPDPAQIFAAVLRILRRGATAEADLAGRHVVVTAGGTREPLDPVRFLGNRSSGKQGYALARAAVARGARVTLVAANVTLTDPAGADVVRVGTTEELRKATVEAAGGADVVVMAAAPADFRPATVAGQKIKKSDEGTAPTIELVTNPDIAAELGAAKRPGQLLVAFAAETHDALENARGKLVKKRADLIVVNEVGVDLVFGADHNTVTILGADGTTTALGELPKDDVSDAIWDQVVTRLDPQVGAAGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W960</accession>
    <name>U5W960_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560863.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31350"/>
    <dbReference type="EnsemblBacteria" id="AGZ44530">
        <property type="protein sequence ID" value="AGZ44530"/>
        <property type="gene ID" value="AFR_31350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6345"/>
    <dbReference type="eggNOG" id="ENOG50329AT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184524_0_0_11"/>
    <dbReference type="OrthoDB" id="2002197at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="7604" checksum="675A5FE8BB5957CE" modified="2014-01-22" version="1">MTDDGVWRDEDKLSVADLSEAMATWDNDGQGDPTGNDAGEEQAFGHDAHVTTERGNTDPDREARPGTTGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W976</accession>
    <name>U5W976_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_31180</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31180"/>
    <dbReference type="EnsemblBacteria" id="AGZ44496">
        <property type="protein sequence ID" value="AGZ44496"/>
        <property type="gene ID" value="AFR_31180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6311"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_33_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="141"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="201"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="452" mass="47762" checksum="02343F690A0012BD" modified="2014-01-22" version="1">MREDHRDKAISAAEQRSAKVAGAIVSGANKKALDATVAAAGEGLLVHPPGNTGNTRAGADDVLDAAKGTEPVVVDVTGGLVLLEPARAGSASASADKTSVVEVFVSDEALSEGTSRDWWILLGIMLGLVIGSVIVVDRLARGAVASVRNLVHAAMAVGGGDLGVRIQPSGPRELAEAGYAFNRMADRLVTSRTSERELVADLSHRLRTPLTVLRLDAEALDPDDTHIGEFSAAELDRRRGIRRIRQAIVTLEGEVDQLINTTRQAVAAQVAAPEEGVCDASEVVRDRMVFWSALAGDQDRQYRVVGAHLRIPVPVARAELAAALDAVLGNVFRYTPQGTAFEVGITRRDGWVAVRVDDAGPGIDDPEKALRRGESEMGSTGLGLDIARRAAQATGGSVSLDRAAMGGASVVMLLADAEASPKQASRFGLVGRLARERDPVKRKWPHQRTDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9C8</accession>
    <name>U5W9C8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative M16-family peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36805</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561953.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36805"/>
    <dbReference type="EnsemblBacteria" id="AGZ45617">
        <property type="protein sequence ID" value="AGZ45617"/>
        <property type="gene ID" value="AFR_36805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7446"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_6_1_11"/>
    <dbReference type="OrthoDB" id="1077305at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="1">
        <location>
            <begin position="31"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="2">
        <location>
            <begin position="185"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="46936" checksum="84099F4C1532699D" modified="2014-01-22" version="1">MSLVATRPGAGEARPYTFPAIQRVSVAGGEVVAAHMPGQHLATAALLLDAGGARETAGREGTATVLAKCLEEGTSARDSAAFALALEGLGAELSTSVDWDAFRVGVSVPSALLPRAVALLAEAARTPRLDPADVLRVRDDEVTGLRMDWAQPGPRADAALRMDLFGAGERYGRPLHGDPTSVAAVTAEDVVEFHKSWFQRAGVLLVAGDLARLDLAELGAAAFAGTSGSPLTPALPLPATVGSARRVLLVDRPGSVQSTLRLGHRAPERAHPDYVPMTLAATVLGGAFTSRLNHLIREVKGYTYGIRADFAMTRRFGRFGVSTSVQTAVTAPALVDTVGEITRTWQEGVTEEELAVGRTWRAGQLTVDMQTPGSIAGSLATLVVHGLPDDYYATLRRDLLDATVADVSAAAQQHLHPEGLTLVVEGDAASIRDELVASGIGEIVDVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9R4</accession>
    <name>U5W9R4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histidinol-phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Histidinol-phosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_38150</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-histidinol phosphate = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562219.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38150"/>
    <dbReference type="EnsemblBacteria" id="AGZ45884">
        <property type="protein sequence ID" value="AGZ45884"/>
        <property type="gene ID" value="AFR_38150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7718"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_4_0_11"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011809">
        <property type="entry name" value="His_9_proposed"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02067">
        <property type="entry name" value="his_9_HisN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ45884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28620" checksum="B6B9DAD679260BDC" modified="2014-01-22" version="1">MAAYADDLALAHLLADTADSISMARFRALDLHVEAKPDLTPVSDADTAVEKAIRGTLARARPRDGVIGEEFGASTPPAGQGNRHWVIDPIDGTKNFIRGVPIWGTLIALMEGERPVAGLVSAPALGRRWWGATGHGSYAGKHQRSATKLSVSAVRKISDASFCYASLNSWEEAGRLEPMLDIMRDSWRSRAYGDFYGYMLLAEGALDIMVEPELSLWDVAALIPIVTEAGGTFTDLDGQRAPGDGSAIATNGHLHEEILGRLARGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9S6</accession>
    <name>U5W9S6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562292.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38520"/>
    <dbReference type="EnsemblBacteria" id="AGZ45958">
        <property type="protein sequence ID" value="AGZ45958"/>
        <property type="gene ID" value="AFR_38520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7794"/>
    <dbReference type="eggNOG" id="ENOG502ZVZW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148720_0_0_11"/>
    <dbReference type="OrthoDB" id="1890385at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12561" checksum="F4A006BAA243126E" modified="2014-01-22" version="1">MSRAGLRTDEELLTVDVPEVIATAMSTADPARQLLIGAAVPAALQAALIGTQPRSMTYLAEIVRRGGLRFAAQLAEPLPTTEQTELVRSWLAVDGDAHVFAGWLDAVAAILEARLSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAA7</accession>
    <name>U5WAA7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arginine exporter protein argO</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_38540</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562296.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38540"/>
    <dbReference type="EnsemblBacteria" id="AGZ45962">
        <property type="protein sequence ID" value="AGZ45962"/>
        <property type="gene ID" value="AFR_38540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7798"/>
    <dbReference type="eggNOG" id="COG1279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087840_0_0_11"/>
    <dbReference type="OrthoDB" id="1953934at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="197" mass="20453" checksum="C7F2565A384DF33F" modified="2014-01-22" version="1">MLTSALAGFAASVVLIVAIGAQNAFVLRQGVRREHVIPVVVICAVSDLMLILAGIAGLGAVVTAQPTAVTIIRWVGAAFLLTYAALAAKRAWRPGALKPADHTPATLRATLLTCLALTWLNPHVYLDTVLLLGSVAQQHPHRWLFGAGAAVASGAWFSALGLGAHRLAPVLSRPAAWRVLDGLIAIVMVAVAVGLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAF1</accession>
    <name>U5WAF1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38740"/>
    <dbReference type="EnsemblBacteria" id="AGZ46002">
        <property type="protein sequence ID" value="AGZ46002"/>
        <property type="gene ID" value="AFR_38740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7839"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_19_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019880">
        <property type="entry name" value="OxyQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03539">
        <property type="entry name" value="DapC_actino"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="28"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="361" mass="37729" checksum="8976F2F2FDBDD14D" modified="2014-01-22" version="1">MSAALPDFPWDLLEPAKAVAAAHPDGLVDLSIGTPVDPVPALIRAALAEASDAPGYPLTAGTPALRTAIAEWLARTCGASGKLGVLPTIGSKELVAWLPTLLGVGPGDVVVIPQVCYPTYEVGVRLAGAEVVRADSLTALGPDPRVKLVWINSPSNPTGRVLPAAHLRKVVDWARERGAVVVSDECYLTLGWDETPVSVLADEVCGGDYTGVVSVHSLSKRSNLAGYRAGFLGGDPALIAELLAVRKHAGMIVPAPVQAAMVAALGDETHVADQRALYAARRESLREALLKAGFEIEHSAAGLYLWSTRGEDCWATVDWLAQRGILAAPGAFYGPSAARHVRIALTATDERVAAAVARLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAP8</accession>
    <name>U5WAP8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="6">2.1.1.182</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">ksgA</name>
        <name evidence="6" type="synonym">rsmA</name>
        <name evidence="9" type="ORF">AFR_39800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>adenosine(1518)/adenosine(1519) in 16S rRNA + 4 S-adenosyl-L-methionine = 4 H(+) + N(6)-dimethyladenosine(1518)/N(6)-dimethyladenosine(1519) in 16S rRNA + 4 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:19609"/>
            <dbReference type="Rhea" id="RHEA-COMP:10232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10233"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.182"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.182" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39800"/>
    <dbReference type="EnsemblBacteria" id="AGZ46214">
        <property type="protein sequence ID" value="AGZ46214"/>
        <property type="gene ID" value="AFR_39800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8052"/>
    <dbReference type="eggNOG" id="COG0030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052908">
        <property type="term" value="F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="rADc" evidence="8">
        <location>
            <begin position="64"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="33974" checksum="190EDDA7F313A377" modified="2014-01-22" version="1">MSSFYRAGPGHRCPVTDRHPRAAVGSDLMADALLGPAEIRELAARLGVAPTKKLGQNFVHDPNTVRRIVAAAGLTAADVVLEVGPGLGSLTLGLLGAAAHVHAVELDPVLAAALPETVAERSDPAKLTVHHADALRIKGSTLSPAPTMLVANLPYNVAVPVVLHLLAELPSLRGGLVMVQKEVADRLVAGPGSKVYGVPSVKLAWYSTARAAGKVPPAVFWPVPNVDSGLVAFTRRDVPADVERRLVFQIVDAAFAQRRKTLRAALAGWAGGAETAEKLLVAAGVSPQARGESLTVEQFAAIAAAAVTAGHTGKLSPSSRRHEEVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAR4</accession>
    <name>U5WAR4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CheW protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562645.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40295"/>
    <dbReference type="EnsemblBacteria" id="AGZ46313">
        <property type="protein sequence ID" value="AGZ46313"/>
        <property type="gene ID" value="AFR_40295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8155"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040928_1_0_11"/>
    <dbReference type="OrthoDB" id="1711729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="2">
        <location>
            <begin position="168"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="2">
        <location>
            <begin position="347"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="491" mass="51178" checksum="BF01548A080CC5BB" modified="2014-01-22" version="1">MTTPALFGVFHAGDLRVALPLDELREVIMRPPSFSPLPATATGLLGAVNLRHVIIPVLDLCRLAGHEDGNTSGKVIVIVARGDQLFGLLADEIEGVVRLTADALLETTVAGDPPALFSHTFERPEDGAVVSLLDAEAISKLPGIPVVRDTGPRGAVTGLGGETADASRRTVLLLRCGPIGLCIDVNHVHSVIPQLTVHSSPLEGTTCRGVVHLHDKAVPAVDPLVLLGLGSVPDDGNLRGLVLAMPRGLVVLTVSDIADIASVPVSDVLPLPPVGMRKGGFLTGVLRSESGDQHLLLDGEALRADAELDALGSLGVSLRGRTETPPPPPVKQQDKDRPDGRQVVPSVRKFLTYSVGMEAATPLSQITEIVPYPPNAIALDGGGPLMAIFTHRRLSVPLLSLQALLGVAGSPDTSTARVLLVDAPGGELIGFVVPALHAIEDSVWEETAPKEPGEPGTMLQRGPLVKIGTEAAGRLLPNVDLTELVAAELVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAT4</accession>
    <name>U5WAT4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_39265</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46107">
        <property type="protein sequence ID" value="AGZ46107"/>
        <property type="gene ID" value="AFR_39265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39265"/>
    <dbReference type="eggNOG" id="COG5006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057295_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="129"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="32304" checksum="FBF49701A143E9B6" modified="2014-01-22" version="1">MLGSATSNQVGAAIGAHAFAAIGPAGVVAVRQLVAAAVLFPLARPNLRRFTWAQWWPTLLLAAAFAIMNLSLYTAIDRIGLGLAVTLEVLGPLGVALAASRTRFDLLCAAGAAAGVYVLVLPGPASDYLGIGLGLLAAACWASYILLNRLLGARLPGIQATAAATAVSALAYLPVAAVLLAQGRLDGRALGYAAAAGVLSSVIPYAADLIALRRVPARFFGMFMSVHPVLAALAGLVLLGQALRVHEWTGILIVIGVLVVNSVRRRSAAAAPRRTATPAGSRCGGNPASWRRGSRTPNAPTARAGTRTTAAHRSCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB07</accession>
    <name>U5WB07_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_39615</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39615"/>
    <dbReference type="EnsemblBacteria" id="AGZ46177">
        <property type="protein sequence ID" value="AGZ46177"/>
        <property type="gene ID" value="AFR_39615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8014"/>
    <dbReference type="eggNOG" id="COG1295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050028_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017039">
        <property type="entry name" value="Virul_fac_BrkB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30213">
        <property type="entry name" value="PTHR30213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03631">
        <property type="entry name" value="Virul_fac_BrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="33776" checksum="BBB98BD18E0F1584" modified="2014-01-22" version="1">MGTVGGVNVIDRAYAAVEARVMALRHRSAYFDHFCRAILRYDEVLGGRLAAAIAYYGFFAVFALALIGYSIFGFLIKSNIGLFEIVQEFLGQNLPFLDVQAILDSGRTVGIVGIVGLVFTGIGWVEAIRSSQRLIWRLNEQPGYIGVRQVVDLLVMIGILLLLAISVFAVYGLEALLAWIADGHVSFLRSAVSLILTVGINMLLAAALLAAVPRLRMTVRRMAPPVLQVGIGITLLNTVGKSFVGLVERNPAYGLVASAVGVLVYLYVFNQLLLFGAAWAATSPHGRVVDLSADDKTAPVGQNTWIRNDRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB66</accession>
    <name>U5WB66_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative phosphosugar isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562790.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41020"/>
    <dbReference type="EnsemblBacteria" id="AGZ46458">
        <property type="protein sequence ID" value="AGZ46458"/>
        <property type="gene ID" value="AFR_41020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8303"/>
    <dbReference type="eggNOG" id="COG2222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_4_0_11"/>
    <dbReference type="OrthoDB" id="1046291at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="32322" checksum="347F1EB3742404AE" modified="2014-01-22" version="1">MSHVKVEIASQPDCWLQAAKLSDSPGLPAAGERVAVAGCGTSWFMAKAYAALREEAGLGETDAFQASEFPLGRHYDRVVTITRSGTTTEVIDLLDALDGRTPTTVITADGAEPAAAVARDAIVLDFADELAVVQTRFATSALSLLRAHLGTDMEPIAADAEVAVRLPLPVHPALAQQITFLGRGWTVGLAEEAALKCRESASWWAEAYPAMDYRHGPISIAGPGRVVWAFGEIPAGLADDVEATGAAFVHSRYNGGYAALGSWCGGRAPLDPMADLVVAQRVAVLLATSQGLDPDHPRNLTRSVVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBJ6</accession>
    <name>U5WBJ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_35570</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561706.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35570"/>
    <dbReference type="EnsemblBacteria" id="AGZ45370">
        <property type="protein sequence ID" value="AGZ45370"/>
        <property type="gene ID" value="AFR_35570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7199"/>
    <dbReference type="eggNOG" id="COG2346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103526_1_0_11"/>
    <dbReference type="OrthoDB" id="1101420at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="SSF46458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16829" checksum="58080A339EAC9381" modified="2014-01-22" version="1">MGETPTLYEWAGGTDAFVRLTDVFYDRVVKDDLLGPLFARMDPGHARYVALWLAEVFGGPPAYSAEHGGYATMVAKHIGMAITEPQRRRWVNLIQDAADEAGLPGDPEFRAAFLGYVEWGTRLARLNSQPGADVVCQAPVPRWGWGVAPPWQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBL4</accession>
    <name>U5WBL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561682.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35450"/>
    <dbReference type="EnsemblBacteria" id="AGZ45346">
        <property type="protein sequence ID" value="AGZ45346"/>
        <property type="gene ID" value="AFR_35450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7174"/>
    <dbReference type="eggNOG" id="ENOG5032UF6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1719952_0_0_11"/>
    <dbReference type="OrthoDB" id="1727996at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16604" checksum="49F3B0EA14AF9CAD" modified="2014-01-22" version="1">MPSKKELVEQIVRLLDIRLLDPLEVLLEGDDAVEALRRLVRVRAARWADQMHDGDDVTAAGAAIRLIATLYPDDRAFDPPVDWWRTPLGAVVARRVGHPAAESVSYATAGAMLGITRQGVHDLVRRGRLPRHPDGGVPSAAVRERLLAQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBP0</accession>
    <name>U5WBP0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562929.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41720"/>
    <dbReference type="EnsemblBacteria" id="AGZ46598">
        <property type="protein sequence ID" value="AGZ46598"/>
        <property type="gene ID" value="AFR_41720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8443"/>
    <dbReference type="HOGENOM" id="CLU_2420413_0_0_11"/>
    <dbReference type="OrthoDB" id="2068791at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="98" mass="10471" checksum="3CB00EF6AF2B4FCE" modified="2014-01-22" version="1">MTPFALLFLLALAGSSCFAIGRALGRGERYEKGYREGFRDGETGSLARAARLIQINERRPIAPALESMLVPSPVPQQLPARSVSPAVVGVPARPQLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC68</accession>
    <name>U5WC68_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_36645</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561921.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36645"/>
    <dbReference type="EnsemblBacteria" id="AGZ45585">
        <property type="protein sequence ID" value="AGZ45585"/>
        <property type="gene ID" value="AFR_36645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7413"/>
    <dbReference type="eggNOG" id="COG0626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_2_11"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080146">
        <property type="term" value="F:L-cysteine desulfhydrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044540">
        <property type="term" value="F:L-cystine L-cysteine-lyase (deaminating)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="367" mass="38065" checksum="B1F0714AFC0D5B56" modified="2014-01-22" version="1">MTYEGDGTRVVHAGLPDPVPGDPFLPGPVFAAPYHLDPVEGPGVNGYGRPDNPTRRALEGAIGSLEGGAVLAFASGQAAITAMLLSTLRTGDTVALPSDGYFTVRAFAQGTLRDLGVKTVLVPTAGPYPSFEGVRLVLLETPANPGLDVCDIRAVAAAAHEAGALVAVDNTTPTPLGQRPLELGADLVVASGTKALTGHSDVLLGYVATNNEDLLAKVTTWRAQTGGVPGAFDSWLAHRSLATLDLRLARQSENAAVLAELLAARPDVQHVRFPGLANDPSYAVASAQMRRIPGIISFDLGSAERVARFLAASELVFAATSFGGLHTTADRRAQWGDDTTPGFVRLSCGIEDATDLVADLTAALDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCC9</accession>
    <name>U5WCC9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563145.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42800"/>
    <dbReference type="EnsemblBacteria" id="AGZ46814">
        <property type="protein sequence ID" value="AGZ46814"/>
        <property type="gene ID" value="AFR_42800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8658"/>
    <dbReference type="HOGENOM" id="CLU_1127189_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="27316" checksum="BEDCB0D94D79B353" modified="2014-01-22" version="1">MVMGGKGSRSSVYVYEYGEAYDDLRTAFFIDERDYNDPELAESALESAEAAMTRAGVIYEVEVTSGIHATRPIGVPTWEAFKQRHFVDGVPLPVRPMQQQLTIPPSWHAMVEAVDTAYHMFRAELMELLAEAMPSGQIAVTWDRGPLRRSPGSVDWSEEKFGFEIRLKAGLVDTSPAEALNSVGTALARRGWALKEPDPEQAEYQASQNLFTVSIRADQTTLMLFGESPLYWSPAEAGSSYVVEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCQ9</accession>
    <name>U5WCQ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_37285</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562046.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37285"/>
    <dbReference type="EnsemblBacteria" id="AGZ45711">
        <property type="protein sequence ID" value="AGZ45711"/>
        <property type="gene ID" value="AFR_37285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7543"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="31331" checksum="3C195A4350B0A708" modified="2014-01-22" version="1">MREPAIEVNGLRKVYGDTVAVDGLDLTVARGEIFALLGPNGAGKTTTVEILEGYRRRDGGDVTVLGVDPAVVHSRGARTDVSARRWRERVGIVLQGTGEFDELTVGEVVNHFARFYPDPDDPASVIDRVGLGAKAKARTHTLSGGQKRRLDVALGIIGRPELLFLDEPTTGFDPEARREFWALIRDLAGGGTTIVLTTHYLEEAETLADRVGVIAAGRLIAVDSPRQLGNRDSALATVSWRTGEGVWEHEQSATPTVLVAELAERFGGEVPGLTVTRPTLEDIYLTMIGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDZ7</accession>
    <name>U5WDZ7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDDEXK_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39955"/>
    <dbReference type="EnsemblBacteria" id="AGZ46245">
        <property type="protein sequence ID" value="AGZ46245"/>
        <property type="gene ID" value="AFR_39955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8084"/>
    <dbReference type="eggNOG" id="COG2887">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084765_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDDEXK_1" evidence="1">
        <location>
            <begin position="28"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="32734" checksum="4FD7BF84FF48515F" modified="2014-01-22" version="1">MPRTSPRSAAVPEQLGFAGMPERLFVCTPSKLGSYADCPRRYRYTYIDRPSPQKGPPWAHNSLGASVHTALKNWYAVTPDRRVPESMPMLLKSTWVREGYRDVDQERIAYRKALGWLDTYVTGLDPEDEPLGVERVVATKTAVLAFNGRADRIDSRVGDDGPEAVIVDYKTGRSGLDADDARGSQALALYAYAAERVFRRPCRRVELHHLPTGTVASHEHTEESLARQVARAEDTARDIMAAEKAVAAGADPDAEFPTNPGTLCSWCDFRRSCPAGQEVAAKEPWASVEHLERPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE69</accession>
    <name>U5WE69_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Polyketide_cyc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562657.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40355"/>
    <dbReference type="EnsemblBacteria" id="AGZ46325">
        <property type="protein sequence ID" value="AGZ46325"/>
        <property type="gene ID" value="AFR_40355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8167"/>
    <dbReference type="eggNOG" id="COG5637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978728_0_0_11"/>
    <dbReference type="OrthoDB" id="1719670at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polyketide_cyc" evidence="2">
        <location>
            <begin position="8"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="270" mass="28688" checksum="A0815B0C5192DB28" modified="2014-01-22" version="1">MVQQAVEIGAPLHAVYQQLETFENYPQFMTGVQQVTRTGDNQTHWVMDVEGHHREFDARITECSLDERVSWATTEGPLLAETITLRPMGETRTQIVAQLEADVAFLLPNDRHGQETLSRRLKADLNAFKGLIETGKLGDLHQLGASSKQLSGTSSTVFSRDRVNPASPASVAARARGTRPHPGAGWGTSAGEFSNANMGTNVDITSAPGITSASDLDDVLAPGRRIGGTGALRGGAAGGAAPMGGKAMSSRDPRRDGVISEEDSGSAHDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEK9</accession>
    <name>U5WEK9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562718.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40660"/>
    <dbReference type="EnsemblBacteria" id="AGZ46386">
        <property type="protein sequence ID" value="AGZ46386"/>
        <property type="gene ID" value="AFR_40660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8229"/>
    <dbReference type="eggNOG" id="ENOG5032HJX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135072_2_1_11"/>
    <dbReference type="OrthoDB" id="2058880at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019662">
        <property type="entry name" value="DUF2516"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10724">
        <property type="entry name" value="DUF2516"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="11103" checksum="2BC75669F148D24A" modified="2014-01-22" version="1">MAYSATPIFYDDVANLISFAVLILSLVVQAVALIHCVTQRGDGFQAIGTLPKGAWLAILAVCIILTLLGVGGGATGLFGLIGVAAALIYLLDVRPGLRDLSDGKGFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF36</accession>
    <name>U5WF36_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">POLIIIAc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562971.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41930"/>
    <dbReference type="EnsemblBacteria" id="AGZ46640">
        <property type="protein sequence ID" value="AGZ46640"/>
        <property type="gene ID" value="AFR_41930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8484"/>
    <dbReference type="eggNOG" id="COG1387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069822_0_0_11"/>
    <dbReference type="OrthoDB" id="1615112at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="DNA_pol_b-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017078">
        <property type="entry name" value="UCP036978_PHPhdr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036978">
        <property type="entry name" value="UCP036978_PHPhdr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="SSF47802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="1">
        <location>
            <begin position="104"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="358" mass="38475" checksum="28EECEED839B76D7" modified="2014-01-22" version="1">MASRDPVADLRRIAFLLERANEATYRVKAFRSAAATVAALPAAELRERVEAGTLAKLQGVGDVTARCVTESLNGEEPAYLRRVESTEGTDLQGAAAALREALRGDCHAHSDWSDGGSPIEEMALAAVELGHEYLVLTDHSPRLTVANGLTAARLRKQLDHVAKVNDALPEGFRILTGIEVDILADGSLDQEDELLARLDVVVGSVHSNLRDDSAKMTRRMLAAIANPHLDILGHLTGRKVSASGAGDKGHRAGRTRPPSDFDVKKVLAACAEHGKAVEINSRPDRLDPPKRMLTVAVEAGCLFSIDTDAHAPGQLDWQRFGCERAALCGVPVDRVVNAWPADRLLEWTAGHAPGNQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP61</accession>
    <name>U5VP61_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357530.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01490"/>
    <dbReference type="EnsemblBacteria" id="AGZ38587">
        <property type="protein sequence ID" value="AGZ38587"/>
        <property type="gene ID" value="AFR_01490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.299"/>
    <dbReference type="eggNOG" id="COG4997">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257651_0_0_11"/>
    <dbReference type="OrthoDB" id="2089688at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="103" mass="11032" checksum="3B554D716E145301" modified="2014-01-22" version="1">MGDVRSEPAAVLAEVAAERRAQDKKWGLQNHPDGTGPAYAAEAALARKECDEAAATGSLSWRHILLEEVAEATAEDDPEALRRELIQVAAVAVAWAEALDRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPB4</accession>
    <name>U5VPB4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357751.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02595"/>
    <dbReference type="EnsemblBacteria" id="AGZ38808">
        <property type="protein sequence ID" value="AGZ38808"/>
        <property type="gene ID" value="AFR_02595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.522"/>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007925_0_0_11"/>
    <dbReference type="OrthoDB" id="212469at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="979" mass="103472" checksum="471E8095A26A92C1" modified="2014-01-22" version="1">MRTASIEKHTVGNALVLHARERISPEAQSLALAVDEDADNDIVVLDLHDDLPVGIWESVAGALHRQRRGIRLVICGAGQDTTVLAGQWLSDRLGRPVIAPYGHMIRGAHGGLFVHAAEGSGWVRYSPGRPPAWEAKRYPTPAWDGAAADFLPTSAVGAVEPLPGGVWIRDTRDQPAIGDHWQRLAAALPCQPQALTIVLGCPGTPPLALDDVARFWRDLGEEGRSRARFVHYGPVQLPEGEPLGQALADLLEAPIVCFTGVPVGRPERPRIHTVTEDGDLGWQVFARELGYTPRVRSTMPARTPKILSYRAPLMLGDPAGPLIYRYAEDAVVEIIQSGLWIRGPVAPRHAERVRARAADPSLHAVVVEDTDPSRVVRLRELAEDLVARLDPPTRSRSAMQLATAVVRTGQRAQAAAGGVVADGSTFRFSKADLALDQIPTPPAAGIEVAAAGSLEVAVPAGLASDELESDRHAAPAVVLGAASRGKAGGSLAAAEPAVPERRVRDSPPSRPGPQQDRSVSVPLWDEASSDVVIPRFDPHPTRLPTRPSGSRSGQASSPGERVITPEVAVRVLEALDQAGGRPVAESVSRSASTVDGSGSVLLEDPPATDYVPAHAGPSRVGPSELTGRGSSDLGSSDLGRFGGAGSRPAELSFDPSDLGRSGSAPSSPALGYSGTVGLGPPLNPPAPTVPGQRDSGPSEPVRFESGKPEDGPAVLPLAATPRHLRFQTTPESDARGLPTPVGLAEERAWLRSTFSREYDAAASSVSRVLSEHPGFQAGPDTMVDAVAVRLYLSALGVGLDAALRDGAIGPQVPFARCVASGLGRLPSHRGATVLSAELSDADLREIRDRGVLTDWGFTSALTEPPPGLPGNVDILIWSMTARRTRLLEPDDDHRADGRVLFLPGTSFKVLDAAEPGSETRGHLLLRELSTDEPDRGHVPFDDLALSSLRRSVEQWARSTSQPALGPAATHRFTTVPGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPX0</accession>
    <name>U5VPX0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357946.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03570"/>
    <dbReference type="EnsemblBacteria" id="AGZ39003">
        <property type="protein sequence ID" value="AGZ39003"/>
        <property type="gene ID" value="AFR_03570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.718"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_11"/>
    <dbReference type="OrthoDB" id="1821409at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="25571" checksum="AF2AA69B25E9D557" modified="2014-01-22" version="1">MTDTQRRVAIVTGGSRGIGRAVSERLAADGQAVVIAYAGNDVEAKATVEAVEAAGGNAVAVQADVADETAVRSLFDLAEQRFGGVDVVVNAAGIMVLSPLADAGLDDLDRMFRTNLRGTFVIGREAARRVRSGGAIINFSTSVTRLNLPTYSGYAATKAAVEALSPILAKELRGRDITVNAVAPGPTATSLFLDGKDQETIDRMAKMNPMERLGRPEDIAEVVSSLAGPARWINGQTIFVNGGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQG7</accession>
    <name>U5VQG7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03740"/>
    <dbReference type="EnsemblBacteria" id="AGZ39037">
        <property type="protein sequence ID" value="AGZ39037"/>
        <property type="gene ID" value="AFR_03740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.754"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_10_6_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="26827" checksum="9702437C6652596E" modified="2014-01-22" version="1">MERLTDQGRPVRVGSRSGKPPFDWNDESTWAPALEGVTAAYITYYPDLALPGAVAAVDGFARLAAHSGVEQLVLLSGRGEPEAEAAERAVQGAGTGWTVVRSSWFSQNFSESFLLDPVQLGDIALPAGDAREPFTDADDIADVVTAALVDSRHRGKVYEVTGPRLLSFAEVAAELSAATGRTITYTPVSRDEYSKVLEENGLPLEFADLFAGILDGRNAHLTDGVQQALGRAPKDFADYARESAAAGAWER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQK6</accession>
    <name>U5VQK6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane lipoprotein lipid attachment site</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04745"/>
    <dbReference type="EnsemblBacteria" id="AGZ39238">
        <property type="protein sequence ID" value="AGZ39238"/>
        <property type="gene ID" value="AFR_04745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.958"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027939">
        <property type="entry name" value="NMT1/THI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31528">
        <property type="entry name" value="PTHR31528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMT1" evidence="1">
        <location>
            <begin position="49"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="335" mass="34676" checksum="7587298DBA3593D9" modified="2014-01-22" version="1">MAAALATTLFAAAGCSDDGDDTSAGSGSQALEKITYLTSFGNFGRDSYAYVAKEKGYFRDAGFDVEIKPGSGSGENIKQIVAGQAQFTPIDLTGGLLAVGGEGKVTGFTAVAAIQQRTMAAIMTLDGYGISSPKDLEGKTLADLPGSVVRNLFPTYAKAANVDPAKVKFVNGAPTTLFGTLAQHKVDGIGQFVVGKPTIEGIAKGKTAVLLPYSDYLQDLYGNALITNSTYAKENPEKVKKFTAALLKGLQDSITSPDEAGTVLKKNVPTADPKSAAAELTLMAPFVRSEASGVPVGALDSQRVARSIAILQGSGQIQAGLTPEQVINFDLVPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR09</accession>
    <name>U5VR09_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative subtilase-family protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_04975</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04975"/>
    <dbReference type="EnsemblBacteria" id="AGZ39284">
        <property type="protein sequence ID" value="AGZ39284"/>
        <property type="gene ID" value="AFR_04975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1005"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_9_0_11"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665979" evidence="4">
        <location>
            <begin position="40"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="5">
        <location>
            <begin position="145"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="579" mass="59723" checksum="E4AA614C9E167701" modified="2014-01-22" version="1">MTVRPFHRTGPTVVVMTLRALAVGFLVGGFALVGAPAVAAPADEAPVSLIVGLRSVTDVVTPLERRVDVVASEPLTGAVTVDVPADQVAEAADVLRADPAVAYVERDQVARIAAVTPDDPYYSDQWGITKTGVNRAWDTTKGSGGIVVAVVDTGVKKLPDLSGRVLAGKDFVNNDSNPDDDQGHGTMTAGVIAAGGNNGVGVAGICWYCKILPVKVLDSSGAGSYSNIAQGIRWSADKGADIINLSLGGASDSQLLRDAVAYATGKGALVIAAAGNDGKSSQHYPAAIPAVLAVGASTSNDSRYSWSNYGSSWVDIAAPGCNPAQARNGLVGQFCGTSSATPFVSGVAALLASTSPAPTSAAIRAALTTTATKISGNWVASSSGRINAATALAVLSGDDHVVPAASFVTPGGNAYVHGTVGVTARATDNIGIARVELLADGVVVATDRVAPYAFSWRTTAPAATVALGLRAYDYSGNVATATRRVTVDNQAPTVRATSTTANGTRSVRKTRYVTATAADAHGIKRVELLVDGKVTYWYAGTVRKFPVQTWKYGKVMTVQVRAYDRAGNVRYAPARKWYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRK5</accession>
    <name>U5VRK5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiosulfate:cyanide sulfurtransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358983.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05815"/>
    <dbReference type="EnsemblBacteria" id="AGZ39452">
        <property type="protein sequence ID" value="AGZ39452"/>
        <property type="gene ID" value="AFR_05815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1181"/>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="32"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="14568" checksum="AFBDF33DB529FFAA" modified="2014-01-22" version="1">MTMSAERAAEIFAERLATQTDVADVQAALTAAVPGFVLVDSRSDAAWAQGHVPGALHLPTAEIAERAAEVLDPAVPVVTYCWGPGCNGATRAALALSIQGYAVREMIGGVEYWIREGFPLRTAGGDVAREADPLVVACGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRM7</accession>
    <name>U5VRM7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="3">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_00480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357329.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00480"/>
    <dbReference type="EnsemblBacteria" id="AGZ38385">
        <property type="protein sequence ID" value="AGZ38385"/>
        <property type="gene ID" value="AFR_00480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.98"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_0_11"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="6">
        <location>
            <begin position="190"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="128"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="482" mass="49877" checksum="B9D5791DA6489AA6" modified="2014-01-22" version="1">MSRIKEFTMPDLGEGLTEGEILSWLVEVGDTIELNQMIAEVETAKAAVELPAKWAGVVTQIFYEAGATVEVGSPIIAIDTDPGAGPLPSAESLAAVEVAPAEGSVEPGMIGGPAPGGRTAVLVGYGPKTATAKRRPRIGTPNGSTTAAPPTPPPAADAGSPRAGVAPNIVTPGPTAAPPAASVRPAGPVLAKPPVRKLARDLGVDLASIAGTGPLGSITRDDVHKAAAPPAPVTARPAVAATAAVFDASREQRIPIKGVRKLTAENMVASAFTAPHVTEFLTVDVTRSMKSLERLKTRPEFRDVRISPLLLVAKAVLLAVKRHPMVNSTWSGATNEIVVKDYVNLGIAAATTRGLIVPNVKDAGRLTLRELADALNALVQTAKSGKTPPSDMSGGTFSISNVGVFGVDTGTPILPPGEAAILAFGAIRPTPWVHKGKIKIRQVTTLGLSFDHRIVDGELGSKFLRDIGRFLEDPEGALLSWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRQ0</accession>
    <name>U5VRQ0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359154.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06670"/>
    <dbReference type="EnsemblBacteria" id="AGZ39623">
        <property type="protein sequence ID" value="AGZ39623"/>
        <property type="gene ID" value="AFR_06670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1363"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053995_2_0_11"/>
    <dbReference type="OrthoDB" id="688156at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="39"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="398" mass="42343" checksum="1C30B94E2EC20C35" modified="2014-01-22" version="1">MLTSARLDVANDLLGLLRDSGTEPRPDTQLEALTLAVAADLPVLLWGEPGIGKTAALGQLAEALDLPLTTVIASVHEPSDFSGLPVIGDDPAVQGVPMAPPDWAVRLVRAGRGLLFLDELSTAPPAVQAALLRVVLERRIGTLRLPPGVRIVAAANPRSSAADGWELSPPLANRFVHLQWVHDHDVVVRGLGGTWPRASLPRLDPALLPDAVAYARRAVCGFLAARPALVHQLPTSETRRGGPWPSPRSWEMALFLVAFATAAGTSREVLSLLVRATVGDGPGLELLAALDRMDLPDPETLLADPGNAVLPERGDLRQAVLDGVVEAVRKRPERSRWEAAWVVLVKAMETGAPDLVVVPAGDLAALRREDWDVPAAIDRLTGAISVSRRADREGVRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS02</accession>
    <name>U5VS02_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06625"/>
    <dbReference type="EnsemblBacteria" id="AGZ39614">
        <property type="protein sequence ID" value="AGZ39614"/>
        <property type="gene ID" value="AFR_06625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1352"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26245" checksum="831085166FBA8380" modified="2014-01-22" version="1">MTGDGTPVLLLHSTVCDRRMWDPQVPVLAGAGCQVIRCDFPGFGETPMPTESQNEADDVSELLDRLDVGATAVIAASGGGQVGLEFASRWPARVTSLILLCTAWGGHERGPELRAFGAREDELLEAGDVAAAVELNVETWVGPAADEATRDRVRVMQRHAFEVQLAAVDVPPSIGHETELKAITAPTLLVSGAHDLPDFRQTAAELAGLLPGSQHVELDWAGHLPSLERPDLVNPLLLSFLRDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS09</accession>
    <name>U5VS09_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359155.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06675"/>
    <dbReference type="EnsemblBacteria" id="AGZ39624">
        <property type="protein sequence ID" value="AGZ39624"/>
        <property type="gene ID" value="AFR_06675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1364"/>
    <dbReference type="eggNOG" id="COG3864">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038906_0_0_11"/>
    <dbReference type="OrthoDB" id="801007at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025154">
        <property type="entry name" value="Put_metallopeptidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018698">
        <property type="entry name" value="VWA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09967">
        <property type="entry name" value="DUF2201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13203">
        <property type="entry name" value="DUF2201_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2201_N" evidence="2">
        <location>
            <begin position="6"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2201" evidence="1">
        <location>
            <begin position="269"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="408" mass="44334" checksum="A28B942314B57857" modified="2014-01-22" version="1">MTTLDSEKLFAARLHAARVRPYLATALFALHPVESRTVPTMAVDRHWRCYISPGFVDRTPLDELAGVWVHEVSHLLRDHHGRGDRYALAHGLSGPGERLRMNIAADFEINDDAYGDGLTQPAGVVLPSLVRLPEGQLMEEYLHEFRLGPHTQNLVWLDCGSGADGLEREWDLGPGDAHGLTDQEQDAVRFRVANGINGRPGRASRNWQRWAEDVVHPPQAWRDLLGAAIRSAVSGGGAGEDYTYSRPARRAASLPKVVLPSLRRKPPRVSVVIDTSGSVSDAELGSALLEVAAIARAVGGRRDLVTVLSCDAAARVVHPLCRAEGIPLVGGGGTDLRTGFTKALRTNPRPDVIVALTDGHTPWPASRPSCRTVIGLFARDTGHSSWSEDDPDYVPKHPPDWARVVSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS61</accession>
    <name>U5VS61_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357449.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01085"/>
    <dbReference type="EnsemblBacteria" id="AGZ38506">
        <property type="protein sequence ID" value="AGZ38506"/>
        <property type="gene ID" value="AFR_01085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.216"/>
    <dbReference type="HOGENOM" id="CLU_2821351_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="66" mass="7476" checksum="C9C813FAD6702A42" modified="2014-01-22" version="1">MTSKAQDPRTTAETMHETAEALERSEDILHRSADNSPDEATRHRLHELGHAVTREAKDIERRADAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS67</accession>
    <name>U5VS67_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable nicotinate-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Deamido-NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Deamido-NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nicotinate mononucleotide adenylyltransferase</fullName>
            <shortName evidence="6">NaMN adenylyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nadD</name>
        <name evidence="8" type="ORF">AFR_07720</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NadD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.18" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07720"/>
    <dbReference type="EnsemblBacteria" id="AGZ39833">
        <property type="protein sequence ID" value="AGZ39833"/>
        <property type="gene ID" value="AFR_07720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1575"/>
    <dbReference type="eggNOG" id="COG1057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069765_1_1_11"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00244">
        <property type="entry name" value="NaMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00482">
        <property type="entry name" value="TIGR00482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="9"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="22019" checksum="6DEC74F60B3DC49E" modified="2014-01-22" version="1">MQKRRRIGIMGGTFDPIHHGHLVAASEVQSRFDLDEVVFVPTGQPYQKGPVSPAEDRYLMTVIATASNPRFQVSRADVDRDGPTYTVDTLRDLHAIFGPATDLFFITGADALAKILSWKDALEMLSLAHFVGVTRPGFELSDEHLPADSVTLVEVPAMAISSSACRARVAEGQPVWYLVPDGVVQYIAKRGLYRPEAPVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS80</accession>
    <name>U5VS80_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359193.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06865"/>
    <dbReference type="EnsemblBacteria" id="AGZ39662">
        <property type="protein sequence ID" value="AGZ39662"/>
        <property type="gene ID" value="AFR_06865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1402"/>
    <dbReference type="eggNOG" id="COG2320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086407_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007344">
        <property type="entry name" value="GrpB/CoaE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34822">
        <property type="entry name" value="PTHR34822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04229">
        <property type="entry name" value="GrpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="18963" checksum="F77FF497610254BF" modified="2014-01-22" version="1">MTIAEPDERWAALGRQACVVLAPLFAEIEHVGSTAVPGLPAKPVIDLMASLEDLDDVNDDALETLGYRMVRTDMPERLFYRREDYDSTAYHLHVVTAESWPTRNERLLRDHLLDHPADRDRYAALKRELMDAHGPGDAYTRGKTALIQELTDAARAERGLPSVPVWEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSW6</accession>
    <name>U5VSW6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative signaling protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_02655</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02655"/>
    <dbReference type="EnsemblBacteria" id="AGZ38820">
        <property type="protein sequence ID" value="AGZ38820"/>
        <property type="gene ID" value="AFR_02655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.534"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="344"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="486"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="735" mass="78261" checksum="55E482CEDB81CCE4" modified="2014-01-22" version="1">MLAGVGGGYVVSLVIVFAASPEVAAIAFGMGILLSTGVGFVAGLRAAGRPGTDRRSRLIWRLIAVSFLFQIATMVLFAVPGAKMTFPAPADGARISFVVLLCVATWAFPLSRATSLERRKSVLDALTVVVSGSMLLWYLILGPYMADRGTSAGIVAAAAVYPMLDLLLIFGLARVLLRGTDSSARRPILLLAVGTLFLFAGDAWVGYMQAHTPDVYRDGKWDFACVLTMHFLFTAAAIDQNRPRTGLASRPGRYRLGGNLPYAAIGVGYGLMIIAAWHEQNRFPWSGLTVGGIAITVLVVLRQIMVQRESDEVAATDGLTGLANRFRFSEVLGRGLDRGGRNGHTTAVLLVDMNGFKQVNDTMGHKAGDALLTAFARMLRDGILGSDLAARLGGDEFAVVLHDIGGVANAEAVARRIAAATDEPVMIDGTPVRVSVSIGIAVSGPAELSPDELLHRADVAMYHAKRRGGATRWACFDDLMGGDDGEPSLEDDLRTAVAGGQLRLLYQPIVGLPDGELIGVEALVRWEHPRRGTIAPDVFIPLAERIGVIGELGLWVLREACTSAQRWPGLQVNVNVSPCQLDEDRFSTDVRAIVRATGMDPGLLVLEVTESALIAEEVPRAHLQILSDEGIRIALDDFGTGYSSLRYLTQLPIDTLKIDRGFVASLDGTAEGAAVAQAVLRLSRVLGLDTVAEGVETAAQARELTLLGGTKAQGFHFARPQPAEQISELVTAALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT87</accession>
    <name>U5VT87_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative rhamnose kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_03280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03280"/>
    <dbReference type="EnsemblBacteria" id="AGZ38945">
        <property type="protein sequence ID" value="AGZ38945"/>
        <property type="gene ID" value="AFR_03280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.660"/>
    <dbReference type="eggNOG" id="COG1070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039395_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008993">
        <property type="term" value="F:rhamnulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019301">
        <property type="term" value="P:rhamnose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07771">
        <property type="entry name" value="FGGY_RhuK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013449">
        <property type="entry name" value="Rhamnulokinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0684">Rhamnose metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="3">
        <location>
            <begin position="65"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="4">
        <location>
            <begin position="244"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="4">
        <location>
            <begin position="436"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="347"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="561" mass="58081" checksum="EE9180EFA8E78314" modified="2014-01-22" version="1">MNTFAAVDLGASSGRVMVGRVEAGRVTLEAVNRFANEPVRTGGTLHWDILGLYRGVLEGLRLAGPVDGIGIDSWAVDYGLLDATGALLGNPVHYRDSRTEGTAAKVSSVVAEDKLYALTGLQKLPFNTIYQLVAAAGTPQLEAARQLLMIPDLLAYWLTGEVGAEFTNASTTQLLDLRSGKWAGELMTSLGIRPSLFPPLRAPGTVIGPVLSSLGIGTPDVIAVGSHDTASAVVGVPAASSNFAYISCGTWSLVGVELDSPVLTEASRLANFTNEGGVDGTVRYLRNVMGLWPLQECLREWGSPDLAALLREAAAAPAFGALVDLDDPIFLPPGDMVARLTQVSKRLPTGRGGDHAASVSERLPTGSGGDRAASVSERLPTGRGADRAALVSERLPTGSGAGGDGAPSVGERLLTGQGEGAAASVGERLLTAGAGGSDRSTPRGAVVRCILDSLALAHRRAVRQAQELSGRHVDAVHIVGGGARNELLCQLTADACGLPVLAGPVEATALGNILVQARTAGAISGDLSALRAVLRETQQIVRYEPRGDAGAWRAAERRLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTS5</accession>
    <name>U5VTS5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 10">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="2 7 10">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 12" type="primary">pgk</name>
        <name evidence="12" type="ORF">AFR_10570</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 10">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7 11">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="2 7 10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360245.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10570"/>
    <dbReference type="EnsemblBacteria" id="AGZ40403">
        <property type="protein sequence ID" value="AGZ40403"/>
        <property type="gene ID" value="AFR_10570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2155"/>
    <dbReference type="eggNOG" id="COG0126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_11"/>
    <dbReference type="OrthoDB" id="462069at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="354"/>
            <end position="357"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ40403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="398" mass="41236" checksum="EDF35A83B7DA2B58" modified="2014-01-22" version="1">MRTLDDLLGEGVTGRRVLVRADLNVPFDKSEAGKISDDGRIRAVLPTLIALRDAGARVIVCSHLGRPKGAPDPKFTLAPVAKRLGELLGSPAAFADDTVGASASAAVDVLGDGQVLLLENLRFNAGETSKDDAERGAFADQLAAFAEYYVDDAFGAVHRKHASVYDVPARLPHFAGGLVLKEVEVLRKVTETPEQPYVVVLGGSKVSDKLAVIKALLPKVDKLLVGGGMCFTFLKAQGHEVGKSLLEAEMIQTCADLLAEADGRIVLPVDVVAATAFDASADHDVVKAEAIPADRLGLDIGPESVALFADAIGSAKTVFWNGPMGVFELAPFAAGTRGVAEAITKIDGFSVVGGGDSAAAVRTLGIDESGFGHISTGGGASLEYLEGKTLPGLAALES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUI2</accession>
    <name>U5VUI2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39385">
        <property type="protein sequence ID" value="AGZ39385"/>
        <property type="gene ID" value="AFR_05480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1111"/>
    <dbReference type="HOGENOM" id="CLU_2271336_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="102" mass="10318" checksum="6DF88C9F0E28AC54" modified="2014-01-22" version="1">MVGLSVAALMLWGVIALIWGPGLIGVSIANCGDTTTAICTPAGKVFGPWSAPVGAGLGLAAIIYGCWIRVREHPALWLTAGLFLTIVGFLITQSLASGAPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUM7</accession>
    <name>U5VUM7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fic family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_05310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05310"/>
    <dbReference type="EnsemblBacteria" id="AGZ39351">
        <property type="protein sequence ID" value="AGZ39351"/>
        <property type="gene ID" value="AFR_05310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1075"/>
    <dbReference type="eggNOG" id="COG3177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047250_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025758">
        <property type="entry name" value="Fic/DOC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040198">
        <property type="entry name" value="Fido_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026287">
        <property type="entry name" value="SoFic-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504">
        <property type="entry name" value="PTHR13504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13784">
        <property type="entry name" value="Fic_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038925">
        <property type="entry name" value="AMP-prot_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="SSF140931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fido" evidence="4">
        <location>
            <begin position="98"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="190"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="191"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR038925-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="38954" checksum="D0F5F805E3F7EA79" modified="2014-01-22" version="1">MKLDLQQESYVAAANATAAVARLDQAAFKLPNPGLLARPAIRREAVATSALEGTYAALDDVLEADFLERDQLTASVSEVVNYVEAAEMAFEWIKDKPITVGMLEQLQKVLVRGTRGDSADAGRIRSTQVFIGAGGGRVGDARFIPPPPGDQLRAGVDAWATWINAGEVFPLIVKMALGHYQFETLHPFNDGNGRLGRLVCVLQLACRGELRVPVLNLSPWLEPRRRDYQDHLLRCSATGEFDAWIRFFCEAVRAQAVRAVEKIDALHEWRDEALKTLEKEHIRGVANRITEDLIGYPMVTPTVAANRNKVTYPAANTAVKRLVQLGILEERTGRQYGRVFAARQVLKIINAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUZ6</accession>
    <name>U5VUZ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_11615</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11615"/>
    <dbReference type="EnsemblBacteria" id="AGZ40612">
        <property type="protein sequence ID" value="AGZ40612"/>
        <property type="gene ID" value="AFR_11615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2366"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_0_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="14"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="371"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="385" mass="41838" checksum="390EC5975E070CC5" modified="2014-01-22" version="1">MMRVLHIITTLSEGGAQQQLRLLVRGLDHHCEIATLSQTGPAGEQLRAGGTTVHEFSSTGDRDLPTVIRLRRLIRLGRFDVVHTHLYRACVQGRFAAWLAGVRHVVATEYHVHGTGPRALYLASERLGRITIAVSPAIAGRLRAWGVPPERIAVIAKGVDAAEFRYDPQLRAAARERLGIAADVPVIGALGRLEPGKRFDRLIRAVGEVPDAVLLLVGDGPARASLERLAVIEGVADRVRFAGAVTHAREMLCAMDVFASPSDEDTFGLVVLEALACGLPALYAVCRPLEELAAARTAVDGTERLTPHDPESLPRKLRTELLSFAERRGARLPARSAGSRYDAAHVAATVGGLYERIAGERPGTRAQRSATRRTTSKRSRRFRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVV0</accession>
    <name>U5VVV0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360996.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13255"/>
    <dbReference type="EnsemblBacteria" id="AGZ40937">
        <property type="protein sequence ID" value="AGZ40937"/>
        <property type="gene ID" value="AFR_13255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2691"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_0_11"/>
    <dbReference type="OrthoDB" id="968232at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="23330" checksum="FFAB4C3FA790DC05" modified="2014-01-22" version="1">MTVTLITGANKGIGYETAKQLLARGHTVYIGARDAERGQKAAAELGANFVQLDVTDDASVGSALSTIGAAEGRLDVLVHNAGVLATTLDGPAALRSFDTNAVGLVRVTEAALPLLRKSSNANVVTISSSAGSFWAVNNPERPEYGLPLALYSASKAAATMLTVQYAKSQPGIKFNALEPGTTATDMTAAFGIGRPVEQSAAVVVRLATLGTDGPTGTFQDESGPLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW10</accession>
    <name>U5VW10_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">zf-CGNR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361217.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14380"/>
    <dbReference type="EnsemblBacteria" id="AGZ41158">
        <property type="protein sequence ID" value="AGZ41158"/>
        <property type="gene ID" value="AFR_14380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2918"/>
    <dbReference type="eggNOG" id="COG5516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087298_2_1_11"/>
    <dbReference type="OrthoDB" id="1478254at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010852">
        <property type="entry name" value="ABATE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023286">
        <property type="entry name" value="ABATE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021005">
        <property type="entry name" value="Znf_CGNR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35525">
        <property type="entry name" value="PTHR35525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07336">
        <property type="entry name" value="ABATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11706">
        <property type="entry name" value="zf-CGNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160904">
        <property type="entry name" value="SSF160904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-CGNR" evidence="1">
        <location>
            <begin position="146"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="191" mass="20094" checksum="EBFFCACA5D2E2450" modified="2014-01-22" version="1">MAGESEPRLVRLMNTIWADRRGVHDSLSTPGELAQWLSATGLTAGESSATAAHLEAAVTLRDALRRLAALKTEDTRPAAASAMQDVETAVAAVNNAAALIPVQPALGLSGGKLHLRPREAPADAGAALTVVAVEAIDLLTGPDGVLNACHAPGCVLYFVKDHPRREWCSTACGNRARAARHYERHRPASAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW54</accession>
    <name>U5VW54_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase domain protein hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359422.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08010"/>
    <dbReference type="EnsemblBacteria" id="AGZ39891">
        <property type="protein sequence ID" value="AGZ39891"/>
        <property type="gene ID" value="AFR_08010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1635"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_18_0_11"/>
    <dbReference type="OrthoDB" id="1484726at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="23111" checksum="DC2D5C5FD55EB82F" modified="2014-01-22" version="1">MWDIDYTLLRGGGVAAQAWRAAFTEVTGVAWRAMPILGGRTDLDICAEVFATHGVTDCTPERFFARYVEEVQGLRHLFVEQGALMPGVRAVLDELGRRGDVVQTLVTGNVPEVAAAKVAAFELDDVFDAEVGGYGTDDSVRATLVRRSLERAEAKYGERFQPVVIGDTAFDVTAALANQALAVAVATGSTSMADLALAGAHAVLPDLSDVDAAVAVITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWA7</accession>
    <name>U5VWA7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbonic anhydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible hydration of carbon dioxide to form bicarbonate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08655"/>
    <dbReference type="EnsemblBacteria" id="AGZ40020">
        <property type="protein sequence ID" value="AGZ40020"/>
        <property type="gene ID" value="AFR_08655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1761"/>
    <dbReference type="eggNOG" id="COG0288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053879_4_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="PTHR11002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="SSF53056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="21645" checksum="BEBEC489D2704DD5" modified="2014-01-22" version="1">MTGSSPADQAGTTTAPARIARPADALAELIAGNRRFVSGLPEHGHEVSAAAAASGGQVPHAVVLGCIDSRVPLEAIFDQTFGSICVVRSGGHVVDRAVLGSVGFAVEALRVPLIMVLGHVRCGAVDATVKALRDGERPEGDVGYLVDEIAPAVNSVGLDDPEVNAKAMRAHVARTVRRMRDVTGVPAAVASGEVAVAGAVYDLDTGLVELLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWG7</accession>
    <name>U5VWG7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative endoribonuclease L-PSP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_14200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361181.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14200"/>
    <dbReference type="EnsemblBacteria" id="AGZ41122">
        <property type="protein sequence ID" value="AGZ41122"/>
        <property type="gene ID" value="AFR_14200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2881"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_1_11"/>
    <dbReference type="OrthoDB" id="1850770at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="PTHR11803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13048" checksum="5E9755174ABA1E6C" modified="2014-01-22" version="1">MRQIVSTPDAPSSPLYSQAVKAGGLVYVSGLVGLDVTTGSMAGPTIGDQTRQALANCRTVLQAADAAWEDIVEVGVLLTDPADFAGMNEEYATWFSASPPTRYVAKLGVELPGVLVSIRMTAFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWX5</accession>
    <name>U5VWX5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_15050</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361351.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15050"/>
    <dbReference type="EnsemblBacteria" id="AGZ41292">
        <property type="protein sequence ID" value="AGZ41292"/>
        <property type="gene ID" value="AFR_15050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3056"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="23995" checksum="7280C7E3F40EDF1F" modified="2014-01-22" version="1">MRVLVVEDFEILARSIGTGLRREGMAVDVVLDGTDALERLAVTRFEVVILDRDLPGVHGDEICRRLAGARSDTRVLMLTAAGTLEDRVEGLGLGADDYLPKPFAFAELVARVRALARRATPPLPPVLSCEDISLDPARRVASRAGRRLELSPKEFALLECLLGTPGLVLSAEELLDRVWDEAADPFTSAVKHTMHRLRAKLGDPPVIHTVREGGYRIGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX73</accession>
    <name>U5VX73_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative permease, DMT superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09860"/>
    <dbReference type="EnsemblBacteria" id="AGZ40261">
        <property type="protein sequence ID" value="AGZ40261"/>
        <property type="gene ID" value="AFR_09860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2011"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="148"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="31643" checksum="F498985E6F483823" modified="2014-01-22" version="1">MIMVLAAAGMFGINGAVSKLVLQAGFTAPQLTLLRAAGAFLGLLVLSAVIRPGVRRLRVTRGELPLLLVYGLSGFFLVPMLYFVAISRIPVGIALLFEYTAPLLVVLWARFGDKQRVRPRLWGGLALSLAGLACVAEVWGDLRLNTVGVVAALVNAVLLAAYYVLGARGVAKRDTLSLTTWAFGAAAVAGLITRAVTAGAGGWEPIADVKIALLCAYVVVFGSILPYLLVAGSLRYLPATSVGILGMAEPVIAAAFAWVTLGASEALNAAQLAGGALVLTGVAIAETARVRLDAPEVRETAGVPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXH2</accession>
    <name>U5VXH2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Response regulator NasT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_10385</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10385"/>
    <dbReference type="EnsemblBacteria" id="AGZ40366">
        <property type="protein sequence ID" value="AGZ40366"/>
        <property type="gene ID" value="AFR_10385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2116"/>
    <dbReference type="eggNOG" id="COG3707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_65_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008327">
        <property type="entry name" value="Sig_transdc_resp-reg_antiterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036382">
        <property type="entry name" value="RR_antiterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="ANTAR" evidence="3">
        <location>
            <begin position="120"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="193" mass="21160" checksum="305E9DCEFB9D3181" modified="2014-01-22" version="1">MLIAEDEALIRLDLAEMLVEEGYDVVGEAGDGETAVRLAEDLTPDLVILDIKMPIMDGLAAAERIAGGRIAPVVILTAFSQRELVERARAAGAMAYLVKPFQKSDLVPAIEIALSRYSEISALESEVAGLTDRLETRKSVERAKGELMTKYSMTEPQAFKWIQRTAMDHRMTMREVADRILAEGEETPGTPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXI8</accession>
    <name>U5VXI8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dephospho-CoA kinase</fullName>
            <ecNumber evidence="3">2.7.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Dephosphocoenzyme A kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">coaE</name>
        <name evidence="4" type="ORF">AFR_10460</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CoaE family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the UPF0157 (GrpB) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the CoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10460"/>
    <dbReference type="EnsemblBacteria" id="AGZ40381">
        <property type="protein sequence ID" value="AGZ40381"/>
        <property type="gene ID" value="AFR_10460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2131"/>
    <dbReference type="eggNOG" id="COG0237">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067032_0_0_11"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007344">
        <property type="entry name" value="GrpB/CoaE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04229">
        <property type="entry name" value="GrpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00152">
        <property type="entry name" value="TIGR00152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="391" mass="41971" checksum="E2C7AA63EBD64397" modified="2014-01-22" version="1">MGLTGGIGAGKSAVAQRLVQRGAILIDSDVLAREVVAAGTDGQAEVVAAFGQEILGADGELDRPALGAKIFGDPAARRHLETIIHPRVRAGAAEMLSSAAPDAIVVNDVPLLVETGIAATFHLVIVVEADTDRRIRRLVETRGMTEEQATARIAAQADDAVRRAAADVVITNNTGFDELDARIEDLWTTRLLPYEENVRLKRHAPHERALHVVPSDPTWPEQAERVIARIRHALGGEAEVSHIGSTAVPGLPAKDLLDLMLAVPTLDDADKAAEALARAGLPQREGEWFDNARGVPGKTWPKRLHGNADPGRSVNLHVRVTGSPGWRFALLMRDHLRADTAARDGYAAAKERWAAQHSDVESYADAKEPWFDAEADAAQSWAEATGWQAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXX7</accession>
    <name>U5VXX7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein tolB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361717.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41622">
        <property type="protein sequence ID" value="AGZ41622"/>
        <property type="gene ID" value="AFR_16720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3390"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_888316_0_0_11"/>
    <dbReference type="OrthoDB" id="821953at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666195" evidence="1">
        <location>
            <begin position="29"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="DPPIV_N" evidence="2">
        <location>
            <begin position="162"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="30913" checksum="0686680CC947F180" modified="2014-01-22" version="1">MANKLSRTLSIVAGFALVTAVQAAPAQAAARPTGVLLLSGGGATHAVDVRSGRVIAEVGDGFDAAVSRRASVAYGRDVDPCHPDVEGCFGAADLLTSSVAGTGERLLVHNPEAQGGVSDPDWSPDGALIVYSWHTPGERGLNLVRADGSGNEQIVSFSGPGTFAPSGRSVAFVKDGNLQVIDLGTRQVRQVTSDGLAAWSAPDWSPDGRFLVYAGESTFFVVPAAGGTPVAAAWPAMMHSVGSPVFSPDGRSVAFTATESFPDPDQPWVRRVFTAAVDGSRLTPVKDTYDEATDWFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY62</accession>
    <name>U5VY62_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11780"/>
    <dbReference type="EnsemblBacteria" id="AGZ40645">
        <property type="protein sequence ID" value="AGZ40645"/>
        <property type="gene ID" value="AFR_11780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2399"/>
    <dbReference type="eggNOG" id="COG4225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_638822_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="SSF50939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665849" evidence="1">
        <location>
            <begin position="23"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="444" mass="48663" checksum="E250D9A75C73F317" modified="2014-01-22" version="1">MRTVMILSVVVLMAVTATPAQAIGKAEAQVMTAATAVAARSDRRPVAGGVHDTVARQTFISWAGQFEDNYVQAYDHRAKTWSTPVRVASGDNDSHNYPTMIQADDGHLLVFRGLHNRELWVARSPQPHSIEGTWTDVNIAEGLGATYPMPFRTADGTIFVFFRETAGDFDPAFPTDTRPMKYVRSTDNGATWQSSTTLTGDRWAIAPLNRPDNMNEIYIGQLRYEPATLLHRERVGIVYTLAGGGPEGHLHDRYHRNIYYTAFDPGTLTFRSASGRSLGTTIDDADQETHLKVAETPLQTVNPRSPDYINLVGTTLGVAPFVVWMQLDAAGVLHTYTGVWSRLRGWQNREVTTGVRVRDMEASGPASWTVYGTPANGAPGISTHRLTAGMTWHDESVIATPKAVQRIEVIGDYRDPARLLATGASSAREVAVADGDIYVAGYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYE1</accession>
    <name>U5VYE1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17595"/>
    <dbReference type="EnsemblBacteria" id="AGZ41797">
        <property type="protein sequence ID" value="AGZ41797"/>
        <property type="gene ID" value="AFR_17595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3566"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_7_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="22698" checksum="BE98C8DFA0766BB9" modified="2014-01-22" version="1">MRGGDRSARADPARLDMTTPFGATLGWGDRPALLLIDMMRAYFTPGSPFDLGSRAAVDGCAELLGVARAAGIPVVHTRVAYAADLTDGGLFVRKVPGLAVLAEGAGHLGEFVPELEPGAGEVVVVKQYASGFFGTSLAATLTARGIDTAVIAGVSTSGCVRATATDALQHGFRPIVAADACGDRTRAVHDASIFDLGAKYADVTTVAEAGAQLRARNRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYG4</accession>
    <name>U5VYG4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362117.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17720"/>
    <dbReference type="EnsemblBacteria" id="AGZ41822">
        <property type="protein sequence ID" value="AGZ41822"/>
        <property type="gene ID" value="AFR_17720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3593"/>
    <dbReference type="eggNOG" id="COG1574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009942_6_1_11"/>
    <dbReference type="OrthoDB" id="751534at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01300">
        <property type="entry name" value="YtcJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033932">
        <property type="entry name" value="YtcJ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="47"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="541" mass="57326" checksum="810FD58D894AB3EC" modified="2014-01-22" version="1">MLTPVPTADLLFTGGSDAWGASTLAVHDGRILAVGEEAREFAGPRTEVVELRGRLLLPGFQDAHVHAVMGGLELGQCDLTGTTDVRVYLERIGAYAEANPDAAWIVGSGWSMESFPGGTPARGLLDDVVRDRPVCLTNRDHHGAWVNSRALELAGITADTLDPPDGRIDREPGGAPAGGLQEGAMSLVARVLPPATPADRLAGLLRAQRLLHSLGITAWQDALLCGTNGYPDISDAYLTAAGDGSLVSAVVGALWWDRESGLEQIAGLVEKRERFTIGPMRGGSVKFMLDGVVENFTAAMTVPYRDACGSPTDNHGLSFIDREELARSVTAVDALGFQAHFHALGDRAVRDALDAVAAARDANGRRDTRPHLAHLQVVHPDDIPRFRTLGATANIQALWAAHEPQMDELTLPFLEPGPAGRQYPFGDLLRAGAPLAAGSDWPVSSPDPLLGIHVAVNRAHLGGDRPAFLPEQALGLRAALGAYTAGSARVNHLDDTGELRPGFRADLVVLDRDPFAGPPAEIGETRVALTVVNGERVYSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYI2</accession>
    <name>U5VYI2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362322.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18355"/>
    <dbReference type="EnsemblBacteria" id="AGZ41949">
        <property type="protein sequence ID" value="AGZ41949"/>
        <property type="gene ID" value="AFR_18355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3725"/>
    <dbReference type="eggNOG" id="ENOG5031XP9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_764252_0_0_11"/>
    <dbReference type="OrthoDB" id="549077at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="362" mass="37850" checksum="CF2EBEFF69D4774E" modified="2014-01-22" version="1">MTAALVSALERKLAGGTFGRGDDIRRTGTAYYRGLGALCAQTCGLDPADLAPAAAPQLESLRRGVLDAALRLLLAGDVPQGRALLAEHAAYRRARQDDPALTADTEPSGPRPAALKAWHGNPAGEELVARFNTSMAGTADTSVVATVPVTTELEAALAEAAAVLTTALPLIGPDVVDGIAVGIYDGAMNSGYYAGTPLVIYVSDRVFADPVRAAEIVLHESLHQKLADLGVARDILRAGYVDESSELLPVPWGGRGRLFSADRSLAAYHVYTHQSLFYLSLLCRGAGDEARLTSSLVKSWARAHHFARGAETPAFAAEYGVDGRRLTAWLAEVVDALAGVPLADGSVLRAHAEGYSGVAVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYL9</accession>
    <name>U5VYL9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360879.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12670"/>
    <dbReference type="EnsemblBacteria" id="AGZ40820">
        <property type="protein sequence ID" value="AGZ40820"/>
        <property type="gene ID" value="AFR_12670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2573"/>
    <dbReference type="eggNOG" id="COG1418">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105441_0_0_11"/>
    <dbReference type="OrthoDB" id="1433958at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="26"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="21113" checksum="36213DFF5E0BF74A" modified="2014-01-22" version="1">MSLGLATLSTTAESLARELLAPLGNRWRHTSGVAARAAELAPLLDVDRDVLVAAAWLHDIGYSDVVRVTGFHPLDGARHLAELSWPAPIPGLVAHHSGARFVAAARGLAEELAAYPDADEHMSDALTYADQTVGPDGTRVTTTQRYTEMICRHGAASWNAQVDSVRRPHLESVAARVHHRLAAHYDRPWSHRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ23</accession>
    <name>U5VZ23_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transport system inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_13200</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360985.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13200"/>
    <dbReference type="EnsemblBacteria" id="AGZ40926">
        <property type="protein sequence ID" value="AGZ40926"/>
        <property type="gene ID" value="AFR_13200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2680"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_11"/>
    <dbReference type="OrthoDB" id="1584669at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="68"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="30210" checksum="BEF90D1FBBB5CAAA" modified="2014-01-22" version="1">MIVTRRETLIGRALLVLLMVLTVLPFISLFVTALHPSGTYPDGLSWPSNPQWGNFADAFSAANMDRLLVSSALIVLGVVPLSVLFGTMAGFAFGHLRMPGHRIGFLVFVLGLTLPFEGLITPLYYQVRDLGLLNTRWAIILPLIGLFMPFSVLWMRAHFVNMPDELSEAARIDGASTWQLFWRVHLPLAKPALSSLAILLFLWTWNQFLLAIVLVDDPAKRTMAGALGAFQGQWGTDIPLLCAGSLLILAPTLIIFLIFQRRFVAALLQGSLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZF2</accession>
    <name>U5VZF2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362750.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20530"/>
    <dbReference type="EnsemblBacteria" id="AGZ42378">
        <property type="protein sequence ID" value="AGZ42378"/>
        <property type="gene ID" value="AFR_20530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4163"/>
    <dbReference type="eggNOG" id="COG4134">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_722939_0_0_11"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665519" evidence="1">
        <location>
            <begin position="34"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="405" mass="42891" checksum="E8ED35E12F3E2126" modified="2014-01-22" version="1">MPPLARRRVVTAAATTALLLLSACSAPDSGGSAQDDGGEVPAKPGKAVTLNILDVAGNLQLTQGMIDDFVSKNSGVVSKVTYSKSPAPELVGKVKAQQNANRVDIDLVLTGVDGLSAGIEQGLWNPLLTKYADRLPGVQEYLPGAAAMQKLAGDSGVTVTYYPSGPLIEYLPAKVPNPPKTAQELLDYAKANPGAVQYARPSNSGPGRTFLMGLPYLLGDSDPKDPTGGWDKTWAYLGELNKYVTLYPSSTGETMKNLANGSAKIIASTTGWDINPRVLGTVPKEAAIGTLEGFHWVTDAHYAVVPKGVSTDKQSAILLLLAHMLTKEQQAKAYDKGYFYPGPAVKDVDLAMAPADSRAAIEEFGRPEYPDLIAKNPLEVPLGSKELVAAFDRWDREIGGAKVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZP0</accession>
    <name>U5VZP0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361170.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41111">
        <property type="protein sequence ID" value="AGZ41111"/>
        <property type="gene ID" value="AFR_14145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2870"/>
    <dbReference type="HOGENOM" id="CLU_2244148_0_0_11"/>
    <dbReference type="OrthoDB" id="2228419at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="104" mass="11105" checksum="5E66FECA55106628" modified="2014-01-22" version="1">MRDPAVELRRVVGKFALLLAFVYVLALVAGVTALFKGTSMAVLGLVILLLPAAAFGVSVRDAVRLHRTSDPARMKVLWPRCALWAGVGSGLLIVAVVVVDRVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZW8</accession>
    <name>U5VZW8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">YfiK-like transcriptional regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362900.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21280"/>
    <dbReference type="EnsemblBacteria" id="AGZ42528">
        <property type="protein sequence ID" value="AGZ42528"/>
        <property type="gene ID" value="AFR_21280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4316"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="134"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="203" mass="21440" checksum="E0AC9D04C26BC6AC" modified="2014-01-22" version="1">MIRLLLADDQALVRGAMAALLDMEPDLKVVAEVGRGDEVVDAVRDHGVEVALLDVEMPGLDGVAAARELRKAVPGCRVLMVTTFGRAGYLRQAMAAGASGFIVKDTPARQLADAVRRVHEGLRVVDPGLAAQSLAHGDSPLTERESDVLRAARDGGTVADIARELHLSEGTVRNHLSSAIGKTGARTRAEAVRLALDNGWLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0D0</accession>
    <name>U5W0D0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15815"/>
    <dbReference type="EnsemblBacteria" id="AGZ41445">
        <property type="protein sequence ID" value="AGZ41445"/>
        <property type="gene ID" value="AFR_15815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3210"/>
    <dbReference type="eggNOG" id="COG3209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003050_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1055" mass="111226" checksum="326CD678D265A921" modified="2014-01-22" version="1">MPCDGVRAFRPCLRESTAVTVPRFLRYFVRTRTRLICTAGLVIAALVAASVPWWPSTSDSPAATAGAPAPAPGAVQFRDREAALTEARQSKKEVLVDSATTGNSQTWALPDGQLRTRTHAVTQQTQDSQGAWKPVDMNLASAKDATPGGLSVRPGNPPTPVRFAGASTPKAGEADTVLAELEIGGRTIAYTWPGALPAPVLDGSRALYPEVLPGVDLLLVARQEGGFGQLLIVKTREAAKNKELASLSYGLRSDSAVFRRNATTGGVRILDAKTKTEVGSIPTPMAWDSSGKDPESPEAPRLSVATDADVLKLSGLGGFEPGSREAPMPTRLDGDGTGSARLHLDVASTGLLSDARVRFPVFLDPPINSGEQAWLFVHKKNPNSNFLNGTSYNGGTTDARVGYESENGGTARSFWRMGFKNIKGAKVSSASFKVLNNHSWSCTSREFQLYFTGAISSGTTWNKQPSWTALQQKKSFAYGYGSNCSDEYVAFNVQNAAQRAADAGATNVTLGMRATSEDDTQTWRKFAATTATLEAVYNTPPKEPTNGVSSPGGACVPGPTGQLGVTVGKTNIVLSAKAVDPDSNLSKLRFQFWKAGTSLPAGTAVTPDSTGKASLTIASSTLVDKTTYAWAVRAEDSSGEESSYFPPGDYACLLTIDATKPPAPDVESDVWLEATPDGATWSTVKFGATGPITFKSDGAKKFEYSFEYVLPTTVTTTTGTYTVPALAPRHSGPTTLVVYAYDAAGNRSDATLYQFYVPPRDVADGPGDVGGDGRADLLVIDASGRLLTLPGDTDGELYGSLSASYSTGGKLNPAGHWLDAATGKAALIAKFGDVYPGDGTTDLFARTPDGGFWLYPGDGYGSFNIDDRLKVLLPSNTPAPSTWTQIKAVGDITGDKLPDLALRAGTAFWVLSGYTGGSFQTATLMEGTAWGRGEIVNIADIDLDGTPDLTWRHLDTGVMSLRHGKPGTVAGSVNLDSLKTAAASRSGDVQYGTGWSASGIYAVIGVPDVNGDRVPDMWAVTGSDGTTRVYHATTTWGGSQKTVLSVDWRTIKAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0F2</accession>
    <name>U5W0F2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362884.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21200"/>
    <dbReference type="EnsemblBacteria" id="AGZ42512">
        <property type="protein sequence ID" value="AGZ42512"/>
        <property type="gene ID" value="AFR_21200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4298"/>
    <dbReference type="eggNOG" id="ENOG50330PX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191540_0_0_11"/>
    <dbReference type="OrthoDB" id="2031580at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046041">
        <property type="entry name" value="DUF5999"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19462">
        <property type="entry name" value="DUF5999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="66" mass="6958" checksum="65BC8A7B837DCA84" modified="2014-01-22" version="1">MCQHLNSCPTAEATDREAARIVATFPEQGWSLLCNGVIVFEDTGELLPDGSTIAPHRGPAVHALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0L9</accession>
    <name>U5W0L9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363193.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22540"/>
    <dbReference type="EnsemblBacteria" id="AGZ42778">
        <property type="protein sequence ID" value="AGZ42778"/>
        <property type="gene ID" value="AFR_22540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4569"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_10_5_11"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665953" evidence="1">
        <location>
            <begin position="32"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="439" mass="46828" checksum="E3DB3563A23F2DA2" modified="2014-01-22" version="1">MTTFTRGRSVRLLAATSAATLLLTACTGGDSDDGKDAAAGYDPAATVELTWWTGQTSEAEKVAEKLAADYHTAHPNVTIKTSAGAPTTDDLLTKLSAGFAGGSYPDISYAFGNWAGDLGASGKTQDLTSFVADPSFAWNEMPEAARTVATADGKVIGVPALVDNLALIYNPKLFDDAGIAYPTDEWSWEDFRAAAKKLTDPGTNTYGTAYSVSGSEDTTWHLWPLLWQNGGKILDGTKPAFNSEAGVKALETLRAMAVDDKSMYLDQTDEKYGPLFNSGRVGMMLSGPWALLDLKDAKLKYGVTALPGVNGDHQTVSGPDLWVLFDHDDANRAGASRDFIKWLTSAEIDAKWNLAVGNLPLRSVEQSTPEFAAYAKEYPGGQKFFDNLANAKQARPTVPGYEVMSRNVGDAIAKVLQGAASPKEALDEAARKSADALEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0T4</accession>
    <name>U5W0T4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_22990</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the DedA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22990"/>
    <dbReference type="EnsemblBacteria" id="AGZ42868">
        <property type="protein sequence ID" value="AGZ42868"/>
        <property type="gene ID" value="AFR_22990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4659"/>
    <dbReference type="eggNOG" id="COG0586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_2_2_11"/>
    <dbReference type="OrthoDB" id="1928533at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032818">
        <property type="entry name" value="DedA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30353">
        <property type="entry name" value="PTHR30353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="21366" checksum="D6E3BB8F9961F6F8" modified="2014-01-22" version="1">MLDHLLGLTSSPWLYLIVLVVVAVDGFIPVVPSETVVIGLGALSANGSPNVVALAAAVVAGSMIGDRISYGLGGRAGGRMTTGKLATAKDKAQRALRRHDGAAILVGRFLPYGRTATTLTSGSMALPMGRFRLFTALAGTAWAIYTIGLGRLGGAAFAGSPLLGTIAGLAFGMSLAGVCALVEKRRRGAPGAKSDRYDLPERSAAAAWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W119</accession>
    <name>U5W119_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cellulose synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16520"/>
    <dbReference type="EnsemblBacteria" id="AGZ41586">
        <property type="protein sequence ID" value="AGZ41586"/>
        <property type="gene ID" value="AFR_16520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3350"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016349_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="94"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="518" mass="57950" checksum="917A4CDA0F27A30A" modified="2014-01-22" version="1">MPLPPNDKEKYLYYGRQGRWIFCVFLLAFAGVMFGMARLAANSMWTSLLYELIALQVAAVFISLLSSTRKRRGSRAGHEALIAAYRPVVHPSVDVFLPSAGEPLSILTNTYRHVAALEWPGPLTVYVLDDSGRDSVAALAHDHGFHYLSRPNRGELKKAGNLRYGYEHSTGDLIHVFDADFAPRADMTRELAPYFEDPAVGIVQSPQFFDVQQDHFNWLQRSAGATQEMFYRWIQPARDAVDAAICVGTNAVYRRSALQAAGGFAQIGHSEDVHTGVNMAKAGFRTRYVPVNLAKGVCPDNFDGFANQQYRWCTGSMSLLSDPGFHRSRLTVKQKLCFWTGFLYYITTAIAAFTSQLPAFLMLWFFPDQIRPLNYLPLIGTIFVWSTLMPRVTDYRWSPAVVRVQMLIGFCHALALLDFLRGRTAAWVPTGAAKRTPTAKRVLRLLRVWLITTQVLTWTGIVLAVRSHGLDLLWATILFAGPMIYFVWPLLLGGRATPERRPAARPAIPGARTSAVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W133</accession>
    <name>U5W133_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_16935</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361805.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16935"/>
    <dbReference type="EnsemblBacteria" id="AGZ41665">
        <property type="protein sequence ID" value="AGZ41665"/>
        <property type="gene ID" value="AFR_16935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3437"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_11"/>
    <dbReference type="OrthoDB" id="1108236at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="23"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="494" mass="50907" checksum="18C842F126D288FC" modified="2014-01-22" version="1">MTTATRELPASTAPDRHTGRWWALVVLAMAQLMVVLDATIVNIALPTAQRDLAFSDAGRQWVVTGYALAFGSLLLLGGRLSDNVGRRKMFLIGLIGFALASALGGAAQGIEMLIVARVLQGAFGAALAPAALSLLSTTFTDPAERGKAFGIFGAISGAGGAVGLLLGGVLTEYASWRWCLYVNLLFAVAAVVGATFKLRDEPVRNRARLDWPGVVTAVGGLVALVYGLGNAETDGWDDPMTYGFIIAGALILIAFVIIERRVANPLLPLRVVIDRNRGGAYAAVAIAGAGMFGVMLFLTYYLTNTLGFTPIQSGLAFLPLLAGVMLTATTVGSTLTPKIGPRPLVPLGALIAAGGMVLLTRLDLDSSYVTGVLPGLVLIGLGMGLIFAPTQNTATSGVQHQDAGVASAMINTAQQIGGSIGTALLSGFAATAATNAMVGRAPGPETAALAAISSYHTVFWWSAGFFLVCAVVAAVVFRTGPLEVDPDAAPVIAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1M6</accession>
    <name>U5W1M6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integral membrane sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_24200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363698.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24200"/>
    <dbReference type="EnsemblBacteria" id="AGZ43108">
        <property type="protein sequence ID" value="AGZ43108"/>
        <property type="gene ID" value="AFR_24200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4902"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025828">
        <property type="entry name" value="Put_sensor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13796">
        <property type="entry name" value="Sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Sensor" evidence="3">
        <location>
            <begin position="21"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="224"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="414" mass="42886" checksum="500B57B2088DA0FB" modified="2014-01-22" version="1">MTVQLGSRDLVSGRPWRAAGYLLVSGLTGVVTLLAAVLLLTAGTALAVLLVGLPLLAGLALLGLPVARAGRWALRLVDPRPAPGGHRPPAYAGLWPWLTTRLTEPLTWRELGYALLLAVVLGPLDLLVVTLAVAVPAALIASPVLLAATGQVNVLKVWAVTSWPAAFAVCLLGLGALAVCAYGMVVVAGAQAALTRLLLAGPDPGVVTELTRSRIRLVDAFEAERGRIERDLHDGAQQRLVSLTMMLGVARLDAPPGPLEAQLARAHDEAEAVLVDLRELIRGIHPPVLTDFGLPAAVADLADRSVVPVDVTLPLPGRFSPAIESAAYFVVCEALSNLAKHSGAARAEVSGGYRDGRLEVRIHDDGRGGAAAGAGTGLVRLADRVSVVEGRLSLSSPAGGPTVLTVEIPCRPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1P7</accession>
    <name>U5W1P7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="1">PPIase</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_17840</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17840"/>
    <dbReference type="EnsemblBacteria" id="AGZ41846">
        <property type="protein sequence ID" value="AGZ41846"/>
        <property type="gene ID" value="AFR_17840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3619"/>
    <dbReference type="eggNOG" id="COG0652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_8_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5006530883" description="Peptidyl-prolyl cis-trans isomerase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="3">
        <location>
            <begin position="69"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="24606" checksum="6472109378907A7B" modified="2014-01-22" version="1">MVAAVLMAGALGLTGASPAVASGGDPDAPTAGPCAYTPTPDDPAARPVPLPRDPERTPSRGVVEVLLRTNLGPIPLVLDRAAAPCTVQSFVHLTRQRFYDRTICHRLTTYPTLLVLQCGDPSGTGEGGPGYRYADELPTGLPPAPTDPTGERRVYARGVLAMANAGPDTNGSQFFLVYGNSALRPNYTIFGSVTTTGLETLDEVAAGGVTPTAEDPAPLDGPPALRTEIRTARVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1T3</accession>
    <name>U5W1T3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18040"/>
    <dbReference type="EnsemblBacteria" id="AGZ41886">
        <property type="protein sequence ID" value="AGZ41886"/>
        <property type="gene ID" value="AFR_18040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3661"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018489_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="389"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="588" mass="60563" checksum="8C68FAECEE72587A" modified="2014-01-22" version="1">MERVSGRHRRNFSLTGIILASATAAVVVIGAGSWVGYEQLAAQDCSGQVKLNVAAAPEIAGAVRTTAQKWMEGEDAQVGGVCVAVSVEDVESAAGAATIAQRHGVTLTGLGAGQGKGTVPDVWLPDSSMWLLRVSSEAPGFVPTDREPIAESPVVLAMPAPIAQKLGWPKKKLQWKDMLATITGGGNALRPGIVDPTRDAAGLAGLLALGGAAGSDAAGTQKKVFALRALAANSSSIRQDLLEKFPRSVEDVTTALSAAPLSEEDVVAFNAGKPPIQLAALYLDPAPAALNYPFAVMPETDPQKQAAAGAFHDVLRGQGFRDALAAAGLRGPDGAAGAGFIAPAGAPAVAAPVNVVPADAAGQAAAATRAVTGINQVLGSWAAITLPGRVLAVFDVSGSMLEKVPTAGNITRAEVTQRAAAQGLALFDDRWAVGTWIFSTDMVGSRPWKELVPITPLTSGRTELNGAISQIIPKPDGDTGLYDTALAAYRNVQDSWQPGRVNSVILFTDGANENPGGLKRGELLAKLKKLRDPKRPVRLVFIGIGTGVDRNELTQIASATSSGGVFIAEDPARISDIFLEAIATRSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Y4</accession>
    <name>U5W1Y4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18585"/>
    <dbReference type="EnsemblBacteria" id="AGZ41995">
        <property type="protein sequence ID" value="AGZ41995"/>
        <property type="gene ID" value="AFR_18585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3772"/>
    <dbReference type="eggNOG" id="COG2503">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105429_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005519">
        <property type="entry name" value="Acid_phosphat_B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03767">
        <property type="entry name" value="Acid_phosphat_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665603" evidence="2">
        <location>
            <begin position="32"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="21592" checksum="8B77C287C9B958D4" modified="2014-01-22" version="1">MTDMKSLLRYAVVSAAAATACVLAVPAAAHAASPTGAATQVAPLALPPYETWIADVGAVAATASDYLEGRLPDASVRPAIVLDIDNTALQTQYRPGLVSPATEAVLDVAQQASADGAAVFFVTARPEILGWQSEANLRGVGYPFAGIYLRPWFNTQPDAELKTDAREDIESKGYTIVANIGNNTSDLSGGHAERTFKLPDYDGQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2F9</accession>
    <name>U5W2F9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Uronate isomerase</fullName>
            <ecNumber evidence="4 6">5.3.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glucuronate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Uronic isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">uxaC</name>
        <name evidence="7" type="ORF">AFR_25265</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>D-glucuronate = D-fructuronate</text>
            <dbReference type="Rhea" id="RHEA:13049"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="ChEBI" id="CHEBI:59863"/>
            <dbReference type="EC" id="5.3.1.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>aldehydo-D-galacturonate = keto-D-tagaturonate</text>
            <dbReference type="Rhea" id="RHEA:27702"/>
            <dbReference type="ChEBI" id="CHEBI:12952"/>
            <dbReference type="ChEBI" id="CHEBI:17886"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Carbohydrate metabolism; pentose and glucuronate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the metallo-dependent hydrolases superfamily. Uronate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.12" evidence="4 6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363909.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25265"/>
    <dbReference type="EnsemblBacteria" id="AGZ43319">
        <property type="protein sequence ID" value="AGZ43319"/>
        <property type="gene ID" value="AFR_25265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5120"/>
    <dbReference type="eggNOG" id="COG1904">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044465_0_0_11"/>
    <dbReference type="OrthoDB" id="167900at2"/>
    <dbReference type="UniPathway" id="UPA00246"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008880">
        <property type="term" value="F:glucuronate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006064">
        <property type="term" value="P:glucuronate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00675">
        <property type="entry name" value="UxaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003766">
        <property type="entry name" value="Uronate_isomerase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02614">
        <property type="entry name" value="UxaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="467" mass="51609" checksum="84CCCB608D5EB1F7" modified="2014-01-22" version="1">MSDLLFAAEPRQRDIARELYTLARDLPLISPHGHVDPGLLAGDEPFPDPARLLIVPDHYVTRMLLSQGIPLAQLGVPSRTGAEVEQDPRVIWRLLAENWYLFRGTPSRLWLERTLSAVFGVDERLGPDTADQLYDTIAERLTEPEFRPRALFERFGIEVLATTESPLDDLSRHAKLAADGWGGPGGRVITTFRPDDVVDLEFDGWAGNVARLGEITGEDTATYSGYLAALRSRRQAFQAAGATSSDHGHPTARTVRLSPSDAAKIFERGLRGTASAADAETFRAHMLMEFARMSIEDGLVLQLHPGAVRNHNKSLYAVHGRDVGGDIPRATDYVHDLAPLLDAYGNDPRLRVVLYTLDEDVFSRELAPLAGGYAALYLGAPWWFLDSPDGLRRYRDAVTETAGFYNTAGFADDTRAFCSIPVRHEVARRADAAYLARLVAEGRLPLDEAAETIADLAYHLPKKVFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3G8</accession>
    <name>U5W3G8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560096.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43758">
        <property type="protein sequence ID" value="AGZ43758"/>
        <property type="gene ID" value="AFR_27485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5571"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_17_1_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="17988" checksum="0CBA5F45DCD224F9" modified="2014-01-22" version="1">MRADAQRNRERILDAAEEVFAQLGASASTEEVARRAGVAIGTVFRHFPSKDDLLAAILKRLLARLVQDASELGGADGLFTFFERLVAQAADKRMVVELVGISIPSAVATLGDAVGNLLHQAQAAGTVRRDIALPEVMALLVSLCQGALQGGWPPDLQTRTLAVIYAGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3J5</accession>
    <name>U5W3J5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_27660</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560131.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27660"/>
    <dbReference type="EnsemblBacteria" id="AGZ43793">
        <property type="protein sequence ID" value="AGZ43793"/>
        <property type="gene ID" value="AFR_27660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5606"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="46"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="364"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="398"/>
            <end position="405"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ43793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="598" mass="64251" checksum="E8392580BEFD2CA3" modified="2014-01-22" version="1">MESPPVETGRRGRSQTVTAAEKKQAKEVSLRRIGRLFTAHRTPLTLVTAIIVASSVIAMASPFLLREVIDRALPQQDLTLLVWLVAGMVAVAAVTSALGVVQTWISTTVGQRVMHRLRTDVFTHLQRQSVAFFTRTRTGEVQSRITNDIGGMQSVVTSTATSIASNLTTAVATAVAMAALSWQLSLVSLVVLPPAIYLTRRVAKMRKEITAQRQRELADLNVTVEEGLSISGVQLSKTMGTGPALVERFTASSTRLIDLELRSELAGRWRMAAMSIIFAAIPALIYLSAGLPMTAGKLSIGTLVAFTSLQAGLFRPLMGLLGVGVQLTSSLALFARIFEYLDLPVEVDDPAEPVTLTRPVRGHLRFEDVTFAYPGSETAAIAGVSLDVPAGTSLALVGETGSGKSTLAALIARLYDPDAGRITVDGIDLRDLRLADLAGIVGVVSQETYLLHTTVRENLRYARPEATDEQIEQAARAAQIHDLIAGLPDGYDTVVGSRGHRFSGGEKQRIAIARTLLRDPRILVLDEATSALDTSTERAVQRAFDELARGRTTVTIAHRLSTVRDADQIVVVDHGRILESGTHESLVSVGGRYADLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Z0</accession>
    <name>U5W3Z0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362997.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21780"/>
    <dbReference type="EnsemblBacteria" id="AGZ42626">
        <property type="protein sequence ID" value="AGZ42626"/>
        <property type="gene ID" value="AFR_21780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4413"/>
    <dbReference type="eggNOG" id="COG3030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085083_2_1_11"/>
    <dbReference type="OrthoDB" id="2023211at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007313">
        <property type="entry name" value="FxsA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35335">
        <property type="entry name" value="PTHR35335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04186">
        <property type="entry name" value="FxsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="176" mass="18041" checksum="397E59E38444F412" modified="2014-01-22" version="1">MRRGLALLPLGLLLLAVAEISVFVAVVHAIGAGWALLIIAVSSVAGLALLRREGIRGWRAFRSAAEAGRPPGAQVSNSLVGLLGALLLAVPGFISAVAGLLLVLPPGRILARRGVERATERRVSSAVAGDLFGPRHVRVRQSSPVDDPAPADAGPVTAGPPGEIVQGEVVEGEIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W414</accession>
    <name>U5W414_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">6-aminohexanoate-dimer hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560211.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28065"/>
    <dbReference type="EnsemblBacteria" id="AGZ43874">
        <property type="protein sequence ID" value="AGZ43874"/>
        <property type="gene ID" value="AFR_28065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5689"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030169_0_2_11"/>
    <dbReference type="OrthoDB" id="803444at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="83"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="393" mass="43581" checksum="02DA7278D6C51F77" modified="2014-01-22" version="1">MTSTTEQTPIRAAAEPSWWDRQGDLIMRRPFNEWTFTHMSWLMPTERVSRGGAHLPLPLSPCPLDLTYRFEDADHTLTELHRRTFTTAFVVLHHGEIVHETYPGAFAGPRTRMQLFSVSKSVTSLLVGIALADGTIGSIKDRVTDYRPDFLGTAYEETTLAELLDMTSGVGDAEVWDVPDSGIKRFEQAVMGGGSVTDVVRSAPRRAAPGERFNYSTFDAQILGWVLEAATGRSLAAYASERLWQHLGADRDAYYWLTRGRPRTALGAGSLNATPRDLARIGLLMANDGMVAGEQIVPRDWVRRSRGSDQPQLGVGSLGPSGYDHYGYSNQWWTLGDSAFQAFTGVGVHGQYLFVDPGADVVIVKCSAWPTQDDERRDRETITALRRIADHFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W462</accession>
    <name>U5W462_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363072.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22155"/>
    <dbReference type="EnsemblBacteria" id="AGZ42701">
        <property type="protein sequence ID" value="AGZ42701"/>
        <property type="gene ID" value="AFR_22155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4491"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_4_0_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019945">
        <property type="entry name" value="F420_G6P_DH-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023907">
        <property type="entry name" value="Non-F420_Flavin_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03557">
        <property type="entry name" value="F420_G6P_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03885">
        <property type="entry name" value="flavin_revert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="34652" checksum="EB96DA856991F3DE" modified="2014-01-22" version="1">MTEYGIHASHEQIPPAELLAAVVAAERAGFQAAMCSDHFSPWSARQGQSAFAWSWLGAALQATNLSFGVVNAPGQRYHPAIIAQAIGTLGAMFPGRFWAALGSGEFSNEHITGDPWPRKEVREARLLECVDVIRDMLAGKEVTRDGLVTVDRARLWTRPETPPALIGAAVSTKTAAWCAGWADGLVTVNAPEEQLRAMISAYRDAGGTGPVCLQVHLSWAPTEAEAEAIAHDQWRSNIFPPPVCWDLETVEHFDVVSENVTVDQVRKLVNVSADLEEHTELLRGYAALGFDRIYLHHVGQDLNPFIDAFGAKVLPALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4K9</accession>
    <name>U5W4K9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptide ABC transporter ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363489.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23325"/>
    <dbReference type="EnsemblBacteria" id="AGZ42935">
        <property type="protein sequence ID" value="AGZ42935"/>
        <property type="gene ID" value="AFR_23325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4727"/>
    <dbReference type="eggNOG" id="ENOG502ZCJ8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_879360_0_0_11"/>
    <dbReference type="OrthoDB" id="1031980at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041307">
        <property type="entry name" value="WcbI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18588">
        <property type="entry name" value="WcbI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WcbI" evidence="1">
        <location>
            <begin position="30"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="32831" checksum="8148684997378AFD" modified="2014-01-22" version="1">MIETAGPDPRTRHFGVFYGLDEPTGDGDVALVVGNCQAESLRIMLDGAGLRTVRMPAVHELVTGDLPHLDRWLARTTLLISQPIRDDYHGLPLGTAQLAGRIATGGRTVRVPVIRFAGLYPTHAIVRPPSDPGLAPPIVGYHDLRVLAEAAGLTTRTELDPTTVRAIADHSLTQLRTREAAHGTVVISDLFTAPAFTLMRTLNHPGNLVFSALAARVRAALGLPEHPVDPGRPLLDSVHAPREAAVLETWGLDGDERPYWVVDGKIIETGAVREAHLAWYSRHPDAVQAGLLRHADAFRILGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4S4</accession>
    <name>U5W4S4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23660"/>
    <dbReference type="EnsemblBacteria" id="AGZ43000">
        <property type="protein sequence ID" value="AGZ43000"/>
        <property type="gene ID" value="AFR_23660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4794"/>
    <dbReference type="eggNOG" id="ENOG5031VPE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1905533_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="14726" checksum="64DFB98254EF3F1E" modified="2014-01-22" version="1">MEWSATDTETGRFSPTVMRSDLDEVENHFISMRARGEGFLEVSLPTADSPQVALSFRGPHAVVEQLRAVDEDPKSFLLVGDGSVPWDGKVEVPGTEGDSVFTGHFVMSVDHAWDAVRQFVRTGSPAGLGEWYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4T3</accession>
    <name>U5W4T3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363513.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23380"/>
    <dbReference type="EnsemblBacteria" id="AGZ42946">
        <property type="protein sequence ID" value="AGZ42946"/>
        <property type="gene ID" value="AFR_23380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4738"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2033086_0_0_11"/>
    <dbReference type="OrthoDB" id="2227269at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="27"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="12815" checksum="A7B1D00B71BED5CC" modified="2014-01-22" version="1">MLSSPHRTVVAAVVGLDDVVIATVYTHTETDDQAPDLHVITVDGDVDRDTAPYLERALVRGLSVGQRVCCDLSRADFFGSAGAKVLFTAAATGGAFRVRGARGITRLVLDAVGFNRTLLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W524</accession>
    <name>U5W524_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363655.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23985"/>
    <dbReference type="EnsemblBacteria" id="AGZ43065">
        <property type="protein sequence ID" value="AGZ43065"/>
        <property type="gene ID" value="AFR_23985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4859"/>
    <dbReference type="HOGENOM" id="CLU_1275456_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666360" evidence="2">
        <location>
            <begin position="30"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="19023" checksum="F622A3207C007B10" modified="2014-01-22" version="1">MRLRRTAVAAVLAAGIVIPAVSGAAVAFAQPPAPSATAAKATPKPSKTTKPAKPSRTPFAASGTVSSTDVAAGTVTVLVKGGTKDVRRQTVTVTVAANARIEINGRRGTIAGLGAGHRITVTGTRVSGVYTATRISAKGAKTPVVKPSPTVTPSPTVTPSPTPSIEPTSPAPDDDVTEDPEDHEDETSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W563</accession>
    <name>U5W563_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD-dependent oxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560489.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29455"/>
    <dbReference type="EnsemblBacteria" id="AGZ44152">
        <property type="protein sequence ID" value="AGZ44152"/>
        <property type="gene ID" value="AFR_29455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5969"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018354_10_0_11"/>
    <dbReference type="OrthoDB" id="330793at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="2">
        <location>
            <begin position="47"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="470" mass="49219" checksum="341405E057FF162B" modified="2014-01-22" version="1">MTESSNDYPAVVDPAAAAELSHRVAGPVLLPGDKGYAEECATYNPAVPNNPALVVGAAGPSDVQAALAFAKAHDLAVAVMNTGHTTVVANDRTLLITTRRMDEVTIDPVARRARVGAGVRWQQVVDAAAKHGLAPLNGSSPLVGVVGYTLGGGLSATMGRKYGWASDHVTALDIVTADGQVRQATADAEPELFWAVRGSKSNLGIVTALEFELFPVTRLYGGVLIFPGEHAEAVLRAYATLTARAPDELTTSIAFMRLPDVPVFPEPLRGTFTVHVRVSFLGAAAEADALLAPLRTAAPVMIDTVADMPYTDYAAIYSDPEGPAPFAERTLSLSPLTGDLVAELLASIGPAADVPADIIELRHLGGALRDTPAGAGAAGLRTSEFVLWVVAVGGPDLTGPALRWTDGLFAALEPWALEGKHLNFIGQEDLSDEAVRAAYPAATYSRLARAKSGYDPANVFRLNHNIKPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5X5</accession>
    <name>U5W5X5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">5-hydroxyisourate hydrolase</fullName>
            <shortName evidence="6">HIU hydrolase</shortName>
            <shortName evidence="6">HIUHase</shortName>
            <ecNumber evidence="6">3.5.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_31540</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.17" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560901.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31540"/>
    <dbReference type="EnsemblBacteria" id="AGZ44568">
        <property type="protein sequence ID" value="AGZ44568"/>
        <property type="gene ID" value="AFR_31540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6384"/>
    <dbReference type="eggNOG" id="COG2351">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115536_1_0_11"/>
    <dbReference type="OrthoDB" id="1955791at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033971">
        <property type="term" value="F:hydroxyisourate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05822">
        <property type="entry name" value="TLP_HIUase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023418">
        <property type="entry name" value="Thyroxine_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395">
        <property type="entry name" value="PTHR10395"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="SSF49472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00768">
        <property type="entry name" value="TRANSTHYRETIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TR_THY" evidence="7">
        <location>
            <begin position="15"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="13804" checksum="C1CA20AA28A65B1D" modified="2014-01-22" version="1">MPSTDGTAAPFHTRISTHILDTATGDPARDVYVRLERRDSEGWRTIAEGRTDDDGRLRHQVPLHDWQAGGYRLMFYVEPYLGGDAFFPEITVAFHVHDPERHYHVPLLLSRYGYTTYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W609</accession>
    <name>U5W609_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MarR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31445"/>
    <dbReference type="EnsemblBacteria" id="AGZ44549">
        <property type="protein sequence ID" value="AGZ44549"/>
        <property type="gene ID" value="AFR_31445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6364"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="158" mass="17282" checksum="4B48D5D0CD167493" modified="2014-01-22" version="1">MVLGMTEPLSDSQQAAWRAFVESSWALHTRLEDELRAATGLSMADYHVMVVLSEAHEHRVRMGELASQLVFSPSRCTYQIASMVKRGLVRKQSCSDDGRGQEAVLTDAGMAALTAAAPLHLATVRELFIDDLDDAEIATVTRVFGRLGPRLRSLPVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W636</accession>
    <name>U5W636_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycogen operon protein GlgX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31595</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560912.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31595"/>
    <dbReference type="EnsemblBacteria" id="AGZ44579">
        <property type="protein sequence ID" value="AGZ44579"/>
        <property type="gene ID" value="AFR_31595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6395"/>
    <dbReference type="eggNOG" id="COG1523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011725_1_1_11"/>
    <dbReference type="OrthoDB" id="99080at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004133">
        <property type="term" value="F:glycogen debranching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02856">
        <property type="entry name" value="E_set_GDE_Isoamylase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044505">
        <property type="entry name" value="GlgX_Isoamylase_N_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011837">
        <property type="entry name" value="Glycogen_debranch_GlgX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02100">
        <property type="entry name" value="glgX_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="4">
        <location>
            <begin position="132"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="464"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="703" mass="79862" checksum="9E99D59C7287876F" modified="2014-01-22" version="1">MQVWPGHRYPLGATYDGTGTNFAIFSEVAEAVELCLFDPSGNERKVQLHEQDAFVWHAYLPGVEPGQRYGYRVYGPYDPSRGLRCNPHKLLLDPYARAVDADIAWHPSLYAYDFDNPDEMSDLDSAPHMAKGVVVNPYFDWGNDRRPDIPYHHSVIYETHVKGLTERHPEVPRDLRGTYAAIGHPAIIEHFKKLGVTAVELMPVHQFVHDNRLADLGLRNYWGYNTLGFFAPYHGYSAMGSLGQQTQEFRGMVKALHAAGMEVILDVVYNHTAEGNHLGPTMSLKGIDNRTYYRLVDDQPNFYMDYTGTGNSLNVRSPQSLQLIMDSLRYWVTEMHVDGFRFDLASTLAREFYDVDRLSTFFEVVQQDPVVGQVKLIAEPWDVGPGGYQVGNFPPNWTEWNGKYRDTVRDFWRGEPATLAEFASRITGSADLYQDDGRKPFHSINFVTAHDGFTLNDLVSYNDKHNEANGEENRDGESHNRSWNSGVEGPTDDKQVLQLRAKQRRNYLATLMLSQGVPMISHGDELGRTQSGNNNAYCQDNEISWIDWENADETLLEFTRKLTAFRHRHQVFQRRRFFTGLPVTARGGGDPLPDLEWFTPDGRPMAGDDWGNDFGRAVALFVNGEGIRERGQYGQRHVDSSFLLFFNAHDAPIEFHTPPTEYGEKWEKVIETAEPSPDRPSVVEAGSSLLVPDRSLIVLDRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6A0</accession>
    <name>U5W6A0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560860.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31335"/>
    <dbReference type="EnsemblBacteria" id="AGZ44527">
        <property type="protein sequence ID" value="AGZ44527"/>
        <property type="gene ID" value="AFR_31335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6342"/>
    <dbReference type="eggNOG" id="COG1233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019327_1_0_11"/>
    <dbReference type="OrthoDB" id="453021at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="529" mass="56864" checksum="A9E73FA73A0C172E" modified="2014-01-22" version="1">MERADAIVIGAGHNGLVAANLLADAGWSVEVLEATPQPGGAVRSGYVTAPGYLSDLFSSFYPLGYASPVMKNLELQDQGLAWTHSPDVLTHLLPDGRAATINRDLETTMASMEQFAPGDGERWRHAYDDWSSVSPHMLKVLFQPFPPVRSGLGLARQLRVAGSLRLARRLVLSVRELGSELFEGEGAKLALAGCTLHTDLSPEEAGGGVYGWLLAMLGQEYGWPVPVGGAQQITAALVRRLEERGGRITYDTPVSRILVAQGKAMGVRTADGRNWRARRAVLADIPAPTLYLDLVGERWLPSRFTEDLEFFRWDGATVKVDWAVNGKIPWKNPAAATAGTVHLGADLNGLTRYAAHLATDEMPPHPFLLMGQMTTADPSHSPEGTESAWAYTHLPHRKHWSPEDIAAHVERMEAVMEEHAPGFGRMVVGRNVFSPADLERENPSLVGGALGGGTAAAFQQLFLRPIPGLGRPDTPVERLFLASASAHPGGGVHGAPGANAARAALARDRALTGRAYQAVINAAHDAIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6G6</accession>
    <name>U5W6G6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD_binding_9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32725"/>
    <dbReference type="EnsemblBacteria" id="AGZ44803">
        <property type="protein sequence ID" value="AGZ44803"/>
        <property type="gene ID" value="AFR_32725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6623"/>
    <dbReference type="eggNOG" id="COG4529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020215_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038732">
        <property type="entry name" value="HpyO_NAD-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13454">
        <property type="entry name" value="NAD_binding_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_9" evidence="1">
        <location>
            <begin position="2"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="389" mass="40754" checksum="71350E8ADC93CCF9" modified="2014-01-22" version="1">MLVEPGVPGGGVAYGTAQPWHLLNSRAGAMSADPDDPDHFVRWAGTTPAAFRPREEYGRYLRDVLDEAAREHPGRLQLRRARAAAITPDGTVALDDGSAVTADHVVLATGGPASARQAIVHPHYVNDPWRPGVLEALPADRPVLLIGTGLTAVDVALTLAADGTRTAPIVAVSRRGLLPLTHTTTAAPPAVPSLDDCATLRDVVRAVRHAAGEAGDWRAIVDGLRPYLDELWTALTPGEQDAFLRHLARPWECHRHRMAPPVAARVAELRADGRLEIRAGGVAAWPGLADYAAVINCAGPGRLPGAAGPLVGGLLAAGLARVGPHGLGLDIDPAGRLIGAGGQVQERLWLIGPLRRGARWETTAVPEIRAQARRLVTDLSAGVPVAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6S3</accession>
    <name>U5W6S3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase</fullName>
            <shortName evidence="3">GMPMT</shortName>
            <ecNumber evidence="3">2.4.99.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">(1-&gt;4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">glgE</name>
        <name evidence="5" type="ORF">AFR_27095</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1-&gt;4)-glucans. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate = [(1-&gt;4)-alpha-D-glucosyl](n+2) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42692"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:10183"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
            <dbReference type="EC" id="2.4.99.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.16" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27095"/>
    <dbReference type="EnsemblBacteria" id="AGZ43680">
        <property type="protein sequence ID" value="AGZ43680"/>
        <property type="gene ID" value="AFR_27095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5491"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015798_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030979">
        <property type="term" value="P:alpha-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02124">
        <property type="entry name" value="GlgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026585">
        <property type="entry name" value="GlgE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021828">
        <property type="entry name" value="GlgE_dom_N/S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF203">
        <property type="entry name" value="PTHR10357:SF203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11896">
        <property type="entry name" value="DUF3416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="4">
        <location>
            <begin position="183"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Maltose 1-phosphate binding" evidence="3">
        <location>
            <begin position="510"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>alpha-maltose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:63576"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="3">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="643" mass="72667" checksum="620C1D113BF1E9EC" modified="2014-01-22" version="1">MSCGRYPAKAVVGELVPISAVSYREGHHSLGVNVVWQGPHGEAEPFTRLVPGEPGLDQWHGVIKPDRVGRWTFTVEAFDDPYLTWRNAVTKKIGAGQGLEDLANDLAEGADVLDLASKIVPQAEEERVRAAAAALRDEDRSLFSRVSPALDLEELLWQHPVRQLVTTTRSYVIWVDRQRALYSSWYEFFPRSEGAEIGPDATPIKHGTFKTASERIPAVAAMGFDVLYLPPIHPIGKINRKGRNNTLVARPGIDVGSPWAIGSDEGGHDAINPLLGTLEDFRDFIGVAHANGLEIAMDLALQAAPDHPWVKTHPEWFTTRADGTIAYAENPPKKYQDIYPINFDNDPKGLREEVLRIVLHWVENGVKIFRVDNPHTKTLNFWQWLISKIKAVDPDVLFLAEAFTRPAMMGGLGKIGFTQSYTYFTWRTTAWEMREYMGQLKASLDYMRPNFWPNTPDILHESLQHGGPPMFKIRTVLASMLSPSWGMYAGLELFEHVNRPGAEEYIDNEKFELKPRDWAGAERTGRTLAPYITKLNAIRRENPALQWLRNLEFHEIDNPSLLCWSKRDPETGNTVLVICSFDSANVQWGNTTLDMPTLGLDWHERFTVTDQISGATYEWGQHNAVRIDPYVEPAHVFVVHPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6V2</accession>
    <name>U5W6V2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364559.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26950"/>
    <dbReference type="EnsemblBacteria" id="AGZ43651">
        <property type="protein sequence ID" value="AGZ43651"/>
        <property type="gene ID" value="AFR_26950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5461"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="101" mass="11636" checksum="29C01D1B0611A79A" modified="2014-01-22" version="1">MPVDAIAVLAEPTRRRILDELRLADSSVSRLVETLAMSQPAVSKHLKVLREAGFVSSRTSAQQRIYSLEPEQFRALDAWLAPYRRLWNRHLDALERHLEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W730</accession>
    <name>U5W730_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27400"/>
    <dbReference type="EnsemblBacteria" id="AGZ43741">
        <property type="protein sequence ID" value="AGZ43741"/>
        <property type="gene ID" value="AFR_27400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5554"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035614_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="351" mass="37391" checksum="B88E7FED5031A1D3" modified="2014-01-22" version="1">MFDRNLADGTDLGAAFCVTVDDEVVIDLHGGFADEARTRPWVADTIVGVYSTTKTMAALTALLVADRGELDFAAPVARYWPEFAAAGKAEVTVAQVMSHSSGLCGWREPVTSDDLYDWEKVTALLAAQEPYWKPGTASGYHVVTQGYLIGEVLRRITGRTLGTLFREEIAGPLGADFHIGLPASEGSRVADLIPPADGPPPGTAELWKVVADNPDLDPLLTRTREWQAAEIPAGNGIGNARAVAGIHAILANGGVSQGHRFLSEEGCRRALEEQVSGVDLVLGTTMRWGLGFALGAGLMPNDNTIFWGGYGGSLAIVDLDARTSIAYTPNRMIGGTTGDFRALELAMEFWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W740</accession>
    <name>U5W740_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561155.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32795"/>
    <dbReference type="EnsemblBacteria" id="AGZ44817">
        <property type="protein sequence ID" value="AGZ44817"/>
        <property type="gene ID" value="AFR_32795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6637"/>
    <dbReference type="eggNOG" id="ENOG5031UK4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163906_0_0_11"/>
    <dbReference type="OrthoDB" id="2089797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="238" mass="25575" checksum="54548EFF72961754" modified="2014-01-22" version="1">MNLLEEAPNVMEEIVDLPDPEKQFLVHPLDLPEARVQFRNGWLVGAATSIPVAALAAAIIGYLGQTVVGPLIAFLALSIFGAMASRWATDRAWDYVPRKRQDRNRPLPRSWELGSSAILALVLGVALLLIVFRLDDSDVPLDFRSYTFGMCAVAALLVVADAVVGLFRPAGRRRALASLPGVAVVAVAAVLAYLAWFDGNADGGLLFWGAVTMAGAAVLVGAAKLWDRRRVTVNEQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7J7</accession>
    <name>U5W7J7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28325"/>
    <dbReference type="EnsemblBacteria" id="AGZ43926">
        <property type="protein sequence ID" value="AGZ43926"/>
        <property type="gene ID" value="AFR_28325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5742"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_0_2_11"/>
    <dbReference type="OrthoDB" id="537440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32582" checksum="91DDDB228AAB0DF3" modified="2014-01-22" version="1">MVNADQVASLVRLAGYGPRDPVLVGAQLDDAPPVLLPGGRAPLVYIASLSKQFTAACAALLVRQGRLDVESSLARWMPELPAWAATVRVRHLIHHTSGLSFDEARHAECDRTTAGVLAALDHLDSRPGTEFRYSNAGYVCLATVVERAAGQPLPRFAQDHLFAPLALNDTRYWSGPAPHPPGAAPTDHRHPAPLSLGDGGVWSTTPDLLRWNQALDHDELGISALLHTPGHLDDGTPLDYAWGVGVRTRGNDRIHRHGGSWPGVTAQLVRIPSRRSGFVVLALDNNQDRTAALAEAMLQALTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7P5</accession>
    <name>U5W7P5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560308.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28550"/>
    <dbReference type="EnsemblBacteria" id="AGZ43971">
        <property type="protein sequence ID" value="AGZ43971"/>
        <property type="gene ID" value="AFR_28550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5788"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049704_1_1_11"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035418">
        <property type="entry name" value="AraC-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14525">
        <property type="entry name" value="AraC_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="196"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="34784" checksum="6F35A6BF74CA574B" modified="2014-01-22" version="1">MIGNLETFQDELAASGFPPLVNKLAGAGFRGRITTRDLGPLRLVSLETPESDCAGWERDASDGENMAIKVMIRGRTRIEQGRGDADLGPADLVLLDPTRTFRFESTASAHVTILVPRRELRIRPAQIDRLVGRRIDGSHGPGALVSVLARESARSATEFREAEALRSAAAVIELITVALEARLGDEQPAPDEWLRNRIAGYIEARLADPDLSPLGIAAAHHMSVRRLHKLFEDQPLTVAALIRRRRLERCRAELTAGGRTVTAVAARWGFPDPTHFSKLFKTTYGYNARALVTNNRARTTRTRTAGPEQDGGDQGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7W5</accession>
    <name>U5W7W5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA polymerase sigma factor SigL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_34890</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34890"/>
    <dbReference type="EnsemblBacteria" id="AGZ45234">
        <property type="protein sequence ID" value="AGZ45234"/>
        <property type="gene ID" value="AFR_34890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7060"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="2"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="7">
        <location>
            <begin position="104"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="162" mass="18397" checksum="376A24AFBB26A664" modified="2014-01-22" version="1">MLYEEHAGPLLMFVLRLTGGDRQRAEDIVQETLLRAWRNAHRLGAQGQQSLRPWLVTVARRIAIDDHRSVSARPPETYDRELESFPSTADDTDRVLQLMTVTDALRTLSQSHREILVETYFRGKTVPEAAEVLGLPLGTAKSRVYYALRALRTALQQRGVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7X8</accession>
    <name>U5W7X8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent dethiobiotin synthetase BioD</fullName>
            <ecNumber evidence="5">6.3.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DTB synthetase</fullName>
            <shortName evidence="5">DTBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Dethiobiotin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bioD</name>
        <name evidence="7" type="ORF">AFR_34965</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)-dethiobiotin + ADP + 3 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the dethiobiotin synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.3" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34965"/>
    <dbReference type="EnsemblBacteria" id="AGZ45249">
        <property type="protein sequence ID" value="AGZ45249"/>
        <property type="gene ID" value="AFR_34965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7075"/>
    <dbReference type="eggNOG" id="COG0132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072551_1_0_11"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00161"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="PTHR43210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="60"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="157"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="247"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="285" mass="29743" checksum="034725389DA8CE3C" modified="2014-01-22" version="1">MRTVTDEVIEATVVEPEPEPEPRSEPRSEAQPEPAARPAQRPVEAGEWRGIVLVTGTDTDVGKTIVTSAIAASAQAAGLRVAVVKPGQTGTAEGGPTDIDRVTHLANPHTTRTLASYPEPLAPLAAAKISGLAPLELYEVVDAVRAEGEKHDLVLVEGAGGLLVPMGVRPSGEAWTVADLATTLGVPTIVVSRAGLGTLNHTALTLEALERRGVAAKVVLGAWPAEPELVHWANLTELVPHLVGALPDGAGGMEPGVFQRSAPGWLTPALYGVLDDWRVWADEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8I9</accession>
    <name>U5W8I9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560632.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30175"/>
    <dbReference type="EnsemblBacteria" id="AGZ44295">
        <property type="protein sequence ID" value="AGZ44295"/>
        <property type="gene ID" value="AFR_30175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6110"/>
    <dbReference type="eggNOG" id="ENOG5033SIV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065342_0_0_11"/>
    <dbReference type="OrthoDB" id="1597787at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="290" mass="30463" checksum="39429A13632FBA42" modified="2014-01-22" version="1">MKRRVTPFGLAVVGCLILAGWALWSGGILDGPIARQVRSSSVYAAPGVGLDQAAAERVIGNRRLVVVMLEPGTEDLRQGCDAVRQAAKGTLVLVLSRQDDEFDNYGCALLPGVDDENFGKAAVAETTIASGVDGFADRPVEALKVVAVNYDGLVKAGVVPDGARTISPSLPRYLIAAAAILAVLLGSLILYLGGRRAGRLAAAAAENRQAATDARTEVGAAATVLAQGIIDLDQRYAQGRRSRKFDRQYRKLTADYTGLLTELADGAAPAALTTRIDSLTDRCRKLSATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8J9</accession>
    <name>U5W8J9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative heavy metal-dependent transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561805.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45469">
        <property type="protein sequence ID" value="AGZ45469"/>
        <property type="gene ID" value="AFR_36065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7300"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_7_4_11"/>
    <dbReference type="OrthoDB" id="1661714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="132" mass="14374" checksum="B88E6BF7E7CA8318" modified="2014-01-22" version="1">MSTAFDNADFPAYTMGRAADMIGVTPAFLRSLGTAGLIEPERSLGGHRRYSRHQLQLATRVRQLLDDGILLTAACRIVMLEDRLAAAHRHILALGGTLTAEDLADLPTQTHPDIATTHYYPSPRPDRSPSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8P0</accession>
    <name>U5W8P0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribonuclease HII</fullName>
            <shortName evidence="11">RNase HII</shortName>
            <ecNumber evidence="11">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rnhB</name>
        <name evidence="15" type="ORF">AFR_36290</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 11 12">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11 12">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11 12">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561850.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36290"/>
    <dbReference type="EnsemblBacteria" id="AGZ45514">
        <property type="protein sequence ID" value="AGZ45514"/>
        <property type="gene ID" value="AFR_36290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7344"/>
    <dbReference type="eggNOG" id="COG0164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_1_0_11"/>
    <dbReference type="OrthoDB" id="1685277at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07182">
        <property type="entry name" value="RNase_HII_bacteria_HII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_B">
        <property type="entry name" value="RNase_HII_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022898">
        <property type="entry name" value="RNase_HII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF18">
        <property type="entry name" value="PTHR10954:SF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase_HII" evidence="14">
        <location>
            <begin position="31"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ45514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="272" mass="28903" checksum="39C21032D41C62AF" modified="2014-01-22" version="1">MLSPPRTVVRREGGLYALERALQRRGFRHVAGADEAGRGACAGPLVAAAAILPEGKRGEIDELADSKLLTAAARERVYAEVVARALAWSVVIIPPAEVDGRGLHVCNLAAMRRALASLTTRPEYVLTDGFPVDGLGAPGLAVWKGDRVAACVAAASVLAKVTRDRLMVELDGRFPDYGFAVHKGYITDEHSAALTRHGPCAEHRFSYVNVAAVSGRGNRPPRARRPANLPADTAEPLMLWPAVEGTVGVASGEQPRPPVPVGEDEAMEGEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W932</accession>
    <name>U5W932_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Urease accessory protein UreD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ureD</name>
        <name evidence="5" type="ORF">AFR_30930</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for maturation of urease via the functional incorporation of the urease nickel metallocenter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UreD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560781.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30930"/>
    <dbReference type="EnsemblBacteria" id="AGZ44446">
        <property type="protein sequence ID" value="AGZ44446"/>
        <property type="gene ID" value="AFR_30930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6261"/>
    <dbReference type="eggNOG" id="COG0829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055097_2_0_11"/>
    <dbReference type="OrthoDB" id="1764668at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01384">
        <property type="entry name" value="UreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002669">
        <property type="entry name" value="UreD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01774">
        <property type="entry name" value="UreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0996">Nickel insertion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="27214" checksum="4F83E1999D20D3A5" modified="2014-01-22" version="1">MRAEARIVAEADGRGGTRLAVLRGESPLLLRRTGPRAAGPVTVHLVGGAAGPLRGDDLRLDIEVGPGARLEVRSVAAQLALPGRPGAPPSRLEIRASVAAEATLRWLPEPLIAAAGCHHLAVTRVEVADGGSLLWRDDLVCGRHNEESGDVRADVTVRYSGSTLYRHELAVGPQAPGWQGAAVLGGGRAAGSVVLAGPGRPDPRLLAGEAVLMPLAGPGLLASAVGDDIRRVRAALDPLCVLPGAPQSGTAEPFRRGQAATAIGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W972</accession>
    <name>U5W972_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_31155</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560825.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31155"/>
    <dbReference type="EnsemblBacteria" id="AGZ44491">
        <property type="protein sequence ID" value="AGZ44491"/>
        <property type="gene ID" value="AFR_31155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6306"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="9"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="94"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="18502" checksum="9FC8F50981C6D17A" modified="2014-01-22" version="1">MRFEDFAAARLPALVRYARLLSGDQEQARDIVQDVLARALVRWARIDRADDQYAYVRRMVTNEYLSWRRRRKLPTVPLDLAPEPSAGRPAEHSDELWRLLAGLPRQQRAVLVLRYYEGLTDHEIAEVLGCRPSTVRGYASRALATLRIELRTAPEHEGALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9F0</accession>
    <name>U5W9F0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562109.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37600"/>
    <dbReference type="EnsemblBacteria" id="AGZ45774">
        <property type="protein sequence ID" value="AGZ45774"/>
        <property type="gene ID" value="AFR_37600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7605"/>
    <dbReference type="eggNOG" id="ENOG5032F0U">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177487_0_0_11"/>
    <dbReference type="OrthoDB" id="2056507at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="104" mass="11409" checksum="ED28A952342CD706" modified="2014-01-22" version="1">MADEQINTEAAEGERDRRFHGGAPAHLDDDQLARRTEAERVEAGIDAYDPNDVPPATDEDPQTDPATSEVYQDITAVTARQESEGETAPLSPDNPFPPTRYAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9T5</accession>
    <name>U5W9T5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PABS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_38250</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38250"/>
    <dbReference type="EnsemblBacteria" id="AGZ45904">
        <property type="protein sequence ID" value="AGZ45904"/>
        <property type="gene ID" value="AFR_38250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7738"/>
    <dbReference type="eggNOG" id="COG0421">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068637_2_0_11"/>
    <dbReference type="OrthoDB" id="1613081at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="285" mass="30511" checksum="62ED0718B6DC2E84" modified="2014-01-22" version="1">MAQRKRGERRVETVASGVAELVGDPDRSSGWTLLLDGAPQSHVDLADPTYLEFEYIRRMAAAIDLTAPPGTPLRVLHLGGGALTLPRYVAATRPGSPQRVVEIDGPLVELVRKALPWDPKAKLRVRVGDAREAVTGMRDDGYDVVIVDVFAGSRTPAHLASVEFIREVARVLAPGGRLIANVADGPPLKYARSQVATIRAVLPEACLVADAAVLRGRRFGNLVVIAGRTPLPVTELSRRAAGDWFPGRVETDLDRFTSGQPAATDIDAVPSPAPPEGLFSSRPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Z3</accession>
    <name>U5W9Z3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561076.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32415"/>
    <dbReference type="EnsemblBacteria" id="AGZ44741">
        <property type="protein sequence ID" value="AGZ44741"/>
        <property type="gene ID" value="AFR_32415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6560"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092792_1_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041483">
        <property type="entry name" value="TetR_C_34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17929">
        <property type="entry name" value="TetR_C_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="24136" checksum="564A58445AE443EC" modified="2014-01-22" version="1">MTFQRARSEEQRAARRQAILDTASAMLDEMPVAAVTLNELSRRVGLAKTAMLRYFESREAVLLDLMDDRTATWLTELEQELAVVDPDRSGEERAEQVADVLSRSLAARTVLCDLYGAQGGVLEHNVSVEVVRRHKRASLANLAVMAGHVRKHLPELGDHAEIFCLNALLMGGALSAYTSPPPSLLAVYESEPALAVHQIDLRSALRLTIITSLVGLLPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA00</accession>
    <name>U5WA00_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative branched-chain amino acid ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_38870</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562362.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38870"/>
    <dbReference type="EnsemblBacteria" id="AGZ46028">
        <property type="protein sequence ID" value="AGZ46028"/>
        <property type="gene ID" value="AFR_38870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7866"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_1_1_11"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="29962" checksum="42E0B6B6D3E01B43" modified="2014-01-22" version="1">MDRFVFLTFDGLSRGAVYAAFALALVLIWRATRIVNFAQGAMAVSTAYVGYSVSTATGSYWLGFVVAVVAGLLLGAVTERVVMRFVDHSSPLNGVVVALGLVLVIQGVLGMIYGNEFRPAGAPFSRDAFTVGGVALLSRFDLFVFAAVFVVVALLAVLFTKTAVGLRMRASAFAPDVSRLLGVNVGGMLTLGWALAAAVGSLAGLLVIPTELGLHPNAMDIVFVSSFTAAVVGGLDSAFGAVAGGLIVGLLLSYTSGYLGATVAPIAVLVLLVVVLLGRPGGLFSRSAARAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA70</accession>
    <name>U5WA70_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562402.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39070"/>
    <dbReference type="EnsemblBacteria" id="AGZ46068">
        <property type="protein sequence ID" value="AGZ46068"/>
        <property type="gene ID" value="AFR_39070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7907"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_3_11"/>
    <dbReference type="OrthoDB" id="1617768at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="93" mass="10802" checksum="A0BE944FDA0EB691" modified="2014-01-22" version="1">MARAATTTDAFNAVAEPWRREILDLLVDGERPVNDLVELLGLTQPQVSKHLRVLREVDLVRVRDVGRRRLYRLNPEPLKPLQEWLGQYDFALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAI3</accession>
    <name>U5WAI3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="3 9">2.7.1.180</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_33490</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ApbE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="3 9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561293.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33490"/>
    <dbReference type="EnsemblBacteria" id="AGZ44956">
        <property type="protein sequence ID" value="AGZ44956"/>
        <property type="gene ID" value="AFR_33490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6778"/>
    <dbReference type="eggNOG" id="COG1477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044403_5_0_11"/>
    <dbReference type="OrthoDB" id="2021063at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="5 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ44956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="31139" checksum="07EBBA294DA1B18A" modified="2014-01-22" version="1">MPLVETLPVGAGTAQWQAWGTVARIVVTDPAALDDARELVEAELAAVDAACSRFRPGSELSVACRAGGRPVTVSPLLADLVAAALEAARQTAGDVDPTVGAALCGLGYDRDFAAITGRQVAPAVRIFTAPDWRAVRLHDLSLTVPDGVLLDLGATAKAAAADRAAARVALRMGVGVLVALGGDIATAGPAPEGGWRVLVQDRPGDPRCTVHLPAGAALATSSTAGRTWGRPGELLHHILDPRTGQPAESVWRTVSVAAFSCLRANTLSTAAIVRGQAAPNLLHGVPARLVTGDLDVLRLGGWPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAS4</accession>
    <name>U5WAS4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40345"/>
    <dbReference type="EnsemblBacteria" id="AGZ46323">
        <property type="protein sequence ID" value="AGZ46323"/>
        <property type="gene ID" value="AFR_40345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8165"/>
    <dbReference type="eggNOG" id="ENOG503060T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184972_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="75" mass="7878" checksum="D3D943AF3E7C87E6" modified="2014-01-22" version="1">MMTTMLSPEIVTVPSLVDRCDSCSAAAKAEIALTSGGSLVFCGHHANKNAEKLTRVASRITVEDGFEWAAQSPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAS7</accession>
    <name>U5WAS7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exodeoxyribonuclease 7 small subunit</fullName>
            <ecNumber evidence="3">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Exodeoxyribonuclease VII small subunit</fullName>
            <shortName evidence="3">Exonuclease VII small subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">xseB</name>
        <name evidence="4" type="ORF">AFR_39215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the XseB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562431.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39215"/>
    <dbReference type="EnsemblBacteria" id="AGZ46097">
        <property type="protein sequence ID" value="AGZ46097"/>
        <property type="gene ID" value="AFR_39215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7935"/>
    <dbReference type="eggNOG" id="COG1722">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145918_0_2_11"/>
    <dbReference type="OrthoDB" id="2057189at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00337">
        <property type="entry name" value="Exonuc_7_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003761">
        <property type="entry name" value="Exonuc_VII_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037004">
        <property type="entry name" value="Exonuc_VII_ssu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34137">
        <property type="entry name" value="PTHR34137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02609">
        <property type="entry name" value="Exonuc_VII_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006488">
        <property type="entry name" value="Exonuc_VII_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116842">
        <property type="entry name" value="SSF116842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01280">
        <property type="entry name" value="xseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="67" mass="7577" checksum="D546DC437DE302D0" modified="2014-01-22" version="1">MTDEKLSYEQARTELATVVERLEQGGTSLEESLALWERGEKLADVCQHWLDGARERIEAARAARTDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB80</accession>
    <name>U5WB80_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mycothiol acetyltransferase</fullName>
            <shortName evidence="2">MSH acetyltransferase</shortName>
            <ecNumber evidence="2">2.3.1.189</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Mycothiol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mshD</name>
        <name evidence="4" type="ORF">AFR_40750</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transfer of acetyl from acetyl-CoA to desacetylmycothiol (Cys-GlcN-Ins) to form mycothiol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-alpha-D-glucopyranoside + acetyl-CoA = CoA + H(+) + mycothiol</text>
            <dbReference type="Rhea" id="RHEA:26172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16768"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58887"/>
            <dbReference type="EC" id="2.3.1.189"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. MshD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.189" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562736.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40750"/>
    <dbReference type="EnsemblBacteria" id="AGZ46404">
        <property type="protein sequence ID" value="AGZ46404"/>
        <property type="gene ID" value="AFR_40750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8249"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068014_0_0_11"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035447">
        <property type="term" value="F:mycothiol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010125">
        <property type="term" value="P:mycothiol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01698">
        <property type="entry name" value="MshD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017813">
        <property type="entry name" value="Mycothiol_AcTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021524">
        <property type="entry name" value="MSH_acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03448">
        <property type="entry name" value="mycothiol_MshD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="140"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Acetyl-CoA binding 1" evidence="2">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Acetyl-CoA binding 2" evidence="2">
        <location>
            <begin position="225"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-alpha-D-glucopyranoside</name>
            <dbReference type="ChEBI" id="CHEBI:58887"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-alpha-D-glucopyranoside</name>
            <dbReference type="ChEBI" id="CHEBI:58887"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-alpha-D-glucopyranoside</name>
            <dbReference type="ChEBI" id="CHEBI:58887"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-alpha-D-glucopyranoside</name>
            <dbReference type="ChEBI" id="CHEBI:58887"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-alpha-D-glucopyranoside</name>
            <dbReference type="ChEBI" id="CHEBI:58887"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="30890" checksum="693DE5204ADDEF05" modified="2014-01-22" version="1">MTDVRSQDRLSPAEAADVLSLAAAADEADGVYPISEDGVLGLKGTGRTHLLAYDGDSLAGYAYVDGDSGELVVHPKHRLRGVGTALLTAAGPAARQFWAHGDEEAARAFAGKAGFTRSRVLWQMRRPLADLPDVPLPDGVKVRTFVPGADDEAWLGVNSRAFSHHPEQGRWTADDLRLRFAEPWFDPAGFLLAVDPADDRLLGFHWTKVHPPEGDEPAIGEIYVLGVDPGGHRRGLGAALSIAGLRHLAGLGLSDALLYVDESNEAAVALYRRLGFDIYSTDVMYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBD6</accession>
    <name>U5WBD6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35000"/>
    <dbReference type="EnsemblBacteria" id="AGZ45256">
        <property type="protein sequence ID" value="AGZ45256"/>
        <property type="gene ID" value="AFR_35000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7082"/>
    <dbReference type="HOGENOM" id="CLU_2857519_0_0_11"/>
    <dbReference type="OrthoDB" id="2215302at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="64" mass="7057" checksum="9A38BDDE0AA58CCB" modified="2014-01-22" version="1">MTAATVIVIGYRLGRAHAAWRDLRSAKRDIPAKRKLAWGHTRPLVWGALFLLGTFVAAAYDAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBJ1</accession>
    <name>U5WBJ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562694.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40540"/>
    <dbReference type="EnsemblBacteria" id="AGZ46362">
        <property type="protein sequence ID" value="AGZ46362"/>
        <property type="gene ID" value="AFR_40540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8205"/>
    <dbReference type="eggNOG" id="ENOG5031TMQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2679419_0_0_11"/>
    <dbReference type="OrthoDB" id="2092917at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="78" mass="8246" checksum="A6B75A1165FD702C" modified="2014-01-22" version="1">MIRPSVLLFALLMSAPALYRYVTDEVDISQVLIRFLIAVPIAAVLLAGMRFITAGYGRTEEPGTPATPTPGEPGPGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC50</accession>
    <name>U5WC50_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46793">
        <property type="protein sequence ID" value="AGZ46793"/>
        <property type="gene ID" value="AFR_42695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8641"/>
    <dbReference type="eggNOG" id="ENOG5030HTG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087581_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="233" mass="24358" checksum="BFB687A826AEC11D" modified="2014-01-22" version="1">MSQNGPYPGQGWSAGGSSGSDEPYTEPADPWGDAATHSSPAPDVAWGGQPMSVPPANESSTGYHPYSAAVPNGAPPMWQPPPAAPLPPKRRNTPIIALVIVLGLLICGGLGTAAWLVQRGDNDPTAGRTGSPTASATTNGTVPEPQSSEDARFVAKGQCVRNEGNADDSPDLKIVSCASGTYEVLKRVDGRTTGEADAESKCGKVAKYTKWYFYDSELDSLDFVLCLKEYGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC58</accession>
    <name>U5WC58_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562874.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41440"/>
    <dbReference type="EnsemblBacteria" id="AGZ46542">
        <property type="protein sequence ID" value="AGZ46542"/>
        <property type="gene ID" value="AFR_41440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8385"/>
    <dbReference type="eggNOG" id="ENOG5031MQ8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1480439_0_0_11"/>
    <dbReference type="OrthoDB" id="1751895at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="17606" checksum="C75FD80E936A5F45" modified="2014-01-22" version="1">MALTNRYKTAPGAGTVAALILVLVFGSPWYSDWVTDNTNPETAGGWWLRLLAWPHWRFDSDDSLRSIIIGDLKAILVVVLTLLFLYLLPGSQLARARGTISQFFAGWAAYIFAGGFAALLTTLFLANPSLLGAFRTAGEGATYGLFVGWIIGLATLGGRRGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC99</accession>
    <name>U5WC99_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561882.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36450"/>
    <dbReference type="EnsemblBacteria" id="AGZ45546">
        <property type="protein sequence ID" value="AGZ45546"/>
        <property type="gene ID" value="AFR_36450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7376"/>
    <dbReference type="eggNOG" id="ENOG5033YYY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151275_0_0_11"/>
    <dbReference type="OrthoDB" id="1827290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="101" mass="11648" checksum="EAB6E61276A73EE2" modified="2014-01-22" version="1">MTPEIWRLHAVADDTLLGELRVHERDWPWVHAKFEPAPAFAPLRPLFDEEARLVEADGPVEVWEAVYDRVRAAVRLADPDGVCPAEFILHVEGDDAWWRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCL3</accession>
    <name>U5WCL3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inner membrane protein yidH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_37035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37035"/>
    <dbReference type="EnsemblBacteria" id="AGZ45661">
        <property type="protein sequence ID" value="AGZ45661"/>
        <property type="gene ID" value="AFR_37035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7493"/>
    <dbReference type="eggNOG" id="COG2149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053359_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="6">
        <location>
            <begin position="21"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13584" checksum="657E91AA69BD025D" modified="2014-01-22" version="1">MLDQWFNPQKTPVVGEKPDYRFSLANERTFLAWIRTALALLAGGLACAQFLPPLPIAHLREGIAVALLLLGGVVAVRAVDHWARTERAMRLGTDLPKSRFPAILALFVAVGALLLVVAVLIKAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCT8</accession>
    <name>U5WCT8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Membrane protein insertase YidC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Foldase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Membrane integrase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Membrane protein YidC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AFR_37830</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the Sec translocase complex via SecD. Specifically interacts with transmembrane segments of nascent integral membrane proteins during membrane integration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXA1/ALB3/YidC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562155.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37830"/>
    <dbReference type="EnsemblBacteria" id="AGZ45820">
        <property type="protein sequence ID" value="AGZ45820"/>
        <property type="gene ID" value="AFR_37830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7650"/>
    <dbReference type="eggNOG" id="COG0706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036138_6_0_11"/>
    <dbReference type="OrthoDB" id="524437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ45820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="23392" checksum="1017A8AEEC23DB82" modified="2014-01-22" version="1">MSISAALDAVLGVAHSALEGLGTFLTPTLAIIVFTVLIRLLISPLTWLQIRSAQRGAALAPQLAVLREKHRDDPMVLATETLALQRANGASPFAAMLPALAQAPFFMLMYHLVQSGWAGTLFGVPLAAHLSAGLPVFAVLLALSVALAWWPARRTRMQMVASGSAADPLTRIMGLLPYLTVVMVAWLPLAGGIYLVTSTAWTALEQLVLRRPMDQFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCX5</accession>
    <name>U5WCX5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative lipid kinase yegS-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38055"/>
    <dbReference type="EnsemblBacteria" id="AGZ45865">
        <property type="protein sequence ID" value="AGZ45865"/>
        <property type="gene ID" value="AFR_38055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7698"/>
    <dbReference type="eggNOG" id="COG1597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045532_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="31965" checksum="E7D90CF327756D4E" modified="2014-01-22" version="1">MLVRALRSEVDLTVEYTRKRGHAAALARSAAESGVDVVVTLGGDGTVNEAVNGLMTAEPGLAARGTAPAFRLPALAVVPGGSTNVFARALGLPRDWVEGTGVILDGLRSGRHRVIGLGRADDRYFTFTAGLGLDAAVVRRVEQARLRGRTSTPSLYFRSIFSQIVSGEDRKSPPLSIERPGEEPETGLGTVIIQNTAPWTYVGDRAINPNPDASFDRGLDLLAVRNLYIASTTRTVTQMVSRNADPHGKHVLRMHDQAEFTVIASRPQAFQLDGDYLGERQKVHFLSVPEALRVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDJ9</accession>
    <name>U5WDJ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MarR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39055"/>
    <dbReference type="EnsemblBacteria" id="AGZ46065">
        <property type="protein sequence ID" value="AGZ46065"/>
        <property type="gene ID" value="AFR_39055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7904"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_2_5_11"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="14"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="18158" checksum="485F24EA0B87B909" modified="2014-01-22" version="1">MSATPPPVPLSGEEEAVMRAMGRLMMILPRSLDADLQRDQRMSLSEYQVLRHLSESRSGLMRMSELAAACDMSLSGMTRLAAKLESQGHLRRVPCERDARGFNAVLTDAGLARLQQAWPSHLASVRRHLIDHLNGLDLCTLAKALEGMTDPAGAACQSASHDQHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE14</accession>
    <name>U5WE14_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39535"/>
    <dbReference type="EnsemblBacteria" id="AGZ46161">
        <property type="protein sequence ID" value="AGZ46161"/>
        <property type="gene ID" value="AFR_39535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7998"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074078_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007995">
        <property type="entry name" value="DUF742"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36221">
        <property type="entry name" value="PTHR36221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05331">
        <property type="entry name" value="DUF742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="13537" checksum="810A5775AE54C431" modified="2014-01-22" version="1">MTMPERDEPTGALVRPYAVTRGRTRPKLEIALEALVETTVRGRSTGTGRGGTEHQYIAAMCDGGRVQSLAEIAARMQLPLGVARVIVADMATDGLVAVYEPTSLEDESDAVGTELLERVLSGLRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE55</accession>
    <name>U5WE55_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_40280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562642.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40280"/>
    <dbReference type="EnsemblBacteria" id="AGZ46310">
        <property type="protein sequence ID" value="AGZ46310"/>
        <property type="gene ID" value="AFR_40280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8152"/>
    <dbReference type="eggNOG" id="COG0704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_1_0_11"/>
    <dbReference type="OrthoDB" id="1648549at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="4">
        <location>
            <begin position="17"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="4">
        <location>
            <begin position="122"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="23787" checksum="4CB9CC13C20A1B35" modified="2014-01-22" version="1">MREEFQADLTEVSRLLVTMAESVRAAMRKATTALLTADREAADAVVDRDAEVDEIYRQVEAKVADTIARQAPVASDLRMVITALHISADLERMGDLAEHVAKTALRRHPSPAVPAELRPVFRQMAEVADRMADKITVVLASPNADLAAELEKDDDAMDDLERDLFKVLLHDDWPYGAETAIDGALLGRFYERYADHAVNAGEQMIYLITGEPVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEX5</accession>
    <name>U5WEX5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Maltotriose-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41235"/>
    <dbReference type="EnsemblBacteria" id="AGZ46501">
        <property type="protein sequence ID" value="AGZ46501"/>
        <property type="gene ID" value="AFR_41235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8345"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_10_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665822" evidence="1">
        <location>
            <begin position="25"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="430" mass="46313" checksum="30B08803FA76B2EC" modified="2014-01-22" version="1">MIAAAAALLLTAACTPAPKEQKIADPGTQVSGTVELWHFFTEREAAAIDTVIKDFTSTHPNIKVTVKAGQDDSKMTQAIGAGNGPDIGLSYSTDIVGKFCSSGAWVDLAPYIKRDNVDLNRLNATTRSYTEFDGKRCAMPFLADAYGIFYNKDLFAKAGIAGPPKTLDELTEDAKKLTVKNPDGSLKTVGFLPLFDFYENAAAHMAPAVGAKWLTPDGKSAVGTDPAWKTLMTWQKNLVDWYGYDNLKKFRSSLGEEFSADNAFQRGQVAINIDAEYRLAFMKDQAPGVKYGVAPIPTADPAKYGGGYVTGNIMGISKNAKNPEAAWELIKYLTTDDKTIVKLANSLKNVPTTTGSFSDPGLQVEPEFKVFMDIFANPNSLTSPPAASGPAYQETFGTFVTKWQSGDIKDLDAGLTDADKQINSVMTLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VMW1</accession>
    <name>U5VMW1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_00120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydrogen cyanide + thiosulfate = 2 H(+) + sulfite + thiocyanate</text>
            <dbReference type="Rhea" id="RHEA:16881"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:18022"/>
            <dbReference type="ChEBI" id="CHEBI:18407"/>
            <dbReference type="ChEBI" id="CHEBI:33542"/>
            <dbReference type="EC" id="2.8.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357257.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00120"/>
    <dbReference type="EnsemblBacteria" id="AGZ38313">
        <property type="protein sequence ID" value="AGZ38313"/>
        <property type="gene ID" value="AFR_00120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.25"/>
    <dbReference type="eggNOG" id="COG2897">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031618_1_3_11"/>
    <dbReference type="OrthoDB" id="1385159at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="31"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="175"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="33372" checksum="E5257746150F3B37" modified="2014-01-22" version="1">MSVENDPAPSLQAYAHPDKLVTTEWLAAHLDTPGLVVVESDEDVLLYESGHIPGAVKVDWHLELNDQLTRDYLDPEGFAALCEAKGIGRDDTIVFYGDNFNWWAAYALWVFSLFGHRDVRLLDGGRQKWAAEGRELTRDRTERPKASYPVPVREDGQIRAFRDQVMGHIASGRPLVDVRSPQEYTGELTHMAAYPQEGALRGGHIPGAVSKPWKSAANEDGTFKPADELTKIYRDELGLETGDDVIAYCRIGERSSHTWFVLQHLLGYPQVRNYDGSWTEWGNLVRAPIAKGSEPGGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNF1</accession>
    <name>U5VNF1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357441.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01045"/>
    <dbReference type="EnsemblBacteria" id="AGZ38498">
        <property type="protein sequence ID" value="AGZ38498"/>
        <property type="gene ID" value="AFR_01045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.208"/>
    <dbReference type="eggNOG" id="COG3655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_31_8_11"/>
    <dbReference type="OrthoDB" id="2040098at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13443">
        <property type="entry name" value="HTH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="86" mass="9262" checksum="E0D33B4F2F1C8FF3" modified="2014-01-22" version="1">MPPEEGHRVDVHIDELLAARGMTLTALADEVGLTLANLSILKNGRARAIRFSTLTALCDALGCQPADLFSVRPENSHRRKPAGSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP31</accession>
    <name>U5VP31_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_02145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02145"/>
    <dbReference type="EnsemblBacteria" id="AGZ38718">
        <property type="protein sequence ID" value="AGZ38718"/>
        <property type="gene ID" value="AFR_02145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.434"/>
    <dbReference type="eggNOG" id="COG1171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="3"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="29104" checksum="6063B35675597D95" modified="2014-01-22" version="1">MSIKLETANPVRSFKGRGMELVASRLADQGATAVVCASAGNLGQALGWSGSRRGLDVTVVARRLAPAVKLDRIRSFGANLELVDGDFEVARERAVTLARQRGVRLVEDSLDLDTCEGAATIGLELVSALSPGDVVLIALGGGAMATGIGHVLKTLAPGVEVVCVQPQGAPALTLSWRARRVVTTDSMDTIADGVAGRFPIAAVLDDLLQVADDAVLVREESIIAGMRMLLEHAGLVVEPSAALGVAAVLEDPDRFAGRHVVTVICGSNVDLHAYQGWVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP88</accession>
    <name>U5VP88_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Demethylmenaquinone methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.163</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">menG</name>
        <name evidence="5" type="ORF">AFR_01640</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2-demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9539"/>
            <dbReference type="Rhea" id="RHEA-COMP:9563"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18151"/>
            <dbReference type="ChEBI" id="CHEBI:55437"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.163"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Quinol/quinone metabolism; menaquinone biosynthesis; menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.163" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01640"/>
    <dbReference type="EnsemblBacteria" id="AGZ38617">
        <property type="protein sequence ID" value="AGZ38617"/>
        <property type="gene ID" value="AFR_01640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.330"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_0_0_11"/>
    <dbReference type="UniPathway" id="UPA00079">
        <property type="reaction ID" value="UER00169"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043770">
        <property type="term" value="F:demethylmenaquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102094">
        <property type="term" value="F:S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102955">
        <property type="term" value="F:S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102027">
        <property type="term" value="F:S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="1 4 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0830">Ubiquinone</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24728" checksum="C24B644CB794EB56" modified="2014-01-22" version="1">MTRADLDKQPHEVAEMFDGVAQRYDLTNTVLSFGQDRGWRRATRAALGLRPGERVLDVGAGTGISTEELGRSGAFAVGADLSTGMLRAGRSSGRTVPLVAGDALKLPFPDEAFDAVTISFALRNVVDTGAALRELARVTRPGGRLVVCEFSHPTNTAFRTVYLSYLMRSLPVVARGVSSNPDAYVYLAESIRAWPDQEGLAGRIAAAGPWSRVGWRNLTGGIAALHRATKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPF3</accession>
    <name>U5VPF3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38679">
        <property type="protein sequence ID" value="AGZ38679"/>
        <property type="gene ID" value="AFR_01950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.395"/>
    <dbReference type="eggNOG" id="ENOG5033K2H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033308_0_0_11"/>
    <dbReference type="OrthoDB" id="2051578at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="462" mass="46022" checksum="7D3715486F0965E0" modified="2014-01-22" version="1">MPAGAAPAPCEQAQRYAAQSGSGILRLAKLDLGPAGRQEKPITDVGVGDAKSALVAQSPINSAALARVLDGGPADREDLTDVLQQQAPPSHKKAKSRDIDATEIGPIRLGRGEMTVHAKWDPGMACGAAEGDVTRSEAELNRAGVLEPLVRVPRKVSSVSTTAVEGRGKAARTVASASLTGGDVSVLDGAVKVKVIEPPSLLAGMSLAKGGEVRYLPAVIEVSGKGIKTERLDTAGDNIEVVVDEGTPKSLSGTGEAADDSGDEDTTPGQDSESATAPHDDESPNGAPGANEPADQGDGKVGVLSGLPKMGGLKSASPTPAPGLPAIPDLPLIKETDTEATPAAGPGTRVKVSLGDVRQAASGHAIAAKATAIHIAVTQGSDPQAYGDSGTGVVLDLEMGLLEVAAVSPEPAGGAGVSDVAGSGGGLPITGPRVDVLALSGLALLIAGAAALVFGMRGRPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPJ5</accession>
    <name>U5VPJ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357667.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02175"/>
    <dbReference type="EnsemblBacteria" id="AGZ38724">
        <property type="protein sequence ID" value="AGZ38724"/>
        <property type="gene ID" value="AFR_02175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.440"/>
    <dbReference type="HOGENOM" id="CLU_757857_0_0_11"/>
    <dbReference type="OrthoDB" id="821953at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="365" mass="38145" checksum="3C6E06BAEA50144C" modified="2014-01-22" version="1">MKDITVEGVRRTLQTLADQARPVELGEAALAGAERRRRRALVGGAAAVLLLITVVAVPLGLSSVRRGAAPPAAGQPTTGPRVVTSYVVGGRSFVIDPTTGRYRQAPGGVAAVSPNLDRYANVVTVNDRSTLRIMSTSGDPAPRTLDLAGWAGQPAWSPDGNWLALPIWEVPADELTAHGFHDIYLVDTINLTARRQTLELGAGPGESLRWDETDRLLVTTPGRLSTVRTDGTLVASRALPQGLPCTRQATTMLPAPLFNGRLLLCKLDGSTASLFTVVPSGADEQGSDIGSFTVTDEQRLTPVAWAGGDTVVLSVEALGRNSVNVVRLGYPALGPPAGRLPRGADQILVGSTDGLAGTATAVVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPS9</accession>
    <name>U5VPS9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38809">
        <property type="protein sequence ID" value="AGZ38809"/>
        <property type="gene ID" value="AFR_02600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.523"/>
    <dbReference type="HOGENOM" id="CLU_2893687_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="62" mass="7107" checksum="A22F48000020DBE3" modified="2014-01-22" version="1">MTDAEHVRNAMARFDQVKGVTDAERDEAFENIKVAAKHYGIEMTETSWRQFGKKPHTTNSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPZ1</accession>
    <name>U5VPZ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357820.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02940"/>
    <dbReference type="EnsemblBacteria" id="AGZ38877">
        <property type="protein sequence ID" value="AGZ38877"/>
        <property type="gene ID" value="AFR_02940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.591"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1232871_0_0_11"/>
    <dbReference type="OrthoDB" id="1364972at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="239" mass="25933" checksum="237380E65803EAB1" modified="2014-01-22" version="1">MTDLHTAPHKVQKPGSPGLTTPTEIGVARAAALFERVDPAAAAAHLEPILAADPESAAGWILLARIRLVLDEVDKALDAANRAVALVPEDPRPLAMASRALTLLGRHEEAVTMAYRAVIVEPKNPLWHDRVAWSLLAADRQYADAEQAARTAVGLEPNEAHYYFTHGVTLDALGHADQARQAFLVSLKLEPENPVAQHRLAVLNGESVRAPEKKKRGWKLFGKRGGNSAMPARPEEYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ71</accession>
    <name>U5VQ71_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Citrate synthase (unknown stereospecificity)</fullName>
            <ecNumber evidence="2">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358008.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03870"/>
    <dbReference type="EnsemblBacteria" id="AGZ39063">
        <property type="protein sequence ID" value="AGZ39063"/>
        <property type="gene ID" value="AFR_03870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.782"/>
    <dbReference type="eggNOG" id="COG0372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070533_1_0_11"/>
    <dbReference type="OrthoDB" id="1412360at2"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="27520" checksum="8A7312AC8315EE42" modified="2014-01-22" version="1">MADEGLRFPTAIGTSDEHQIRLLGQDLAADLMGKVGFGELAYWLVAGRRPTPGEVRVFETVLVALADHGFTPTAIAARLTYLSAPESLQGALAAGLLGGGSRFLGVTEDCGRFLAEVLKNGVPTDDAGFDALALEAVTKARAEKKFVPGLGHPVHKQQDPRTPVLIRIADEEGLRGPHLRLFEAIGRVHPQVLNRTLPLNGAGVCGAALADLGLPVDLLRGFALLARAAGLLGHLAEERRNPIGMDVYLTVDRNAVYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQU6</accession>
    <name>U5VQU6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_04625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04625"/>
    <dbReference type="EnsemblBacteria" id="AGZ39214">
        <property type="protein sequence ID" value="AGZ39214"/>
        <property type="gene ID" value="AFR_04625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.934"/>
    <dbReference type="eggNOG" id="COG4758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102484_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012551">
        <property type="entry name" value="DUF1707"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08044">
        <property type="entry name" value="DUF1707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="179" mass="19730" checksum="33811D60F46D74DB" modified="2014-01-22" version="1">MLEGMGRDEMRAADGDRQAVADQLKAALDEGRLDLHEYDERLQQAYAAKTYGELQPLLGDLPSAAVPAPRSPESAALVSASNAELTRRWLLEVWGSWFPVVGITTAIWLISWIASSGENAYFWPIWVAGPWGAFLVFATVAGLASGEPRKQAEKKARKEQAKQLKRERKALAEKEHKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR20</accession>
    <name>U5VR20_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_04840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358294.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04840"/>
    <dbReference type="EnsemblBacteria" id="AGZ39257">
        <property type="protein sequence ID" value="AGZ39257"/>
        <property type="gene ID" value="AFR_04840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.977"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_6_2_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="93"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="39148" checksum="0A71039EDB5BC7AC" modified="2014-01-22" version="1">MSQAKPPVKAGPRRRAKQVGIVGAAIGVAAAGLATAFAVERVLVRKSLNAPGDPHADEGFGDQRFDTELTVTAADGTDLHVEIVEPRTDAGKPTIVFVHGFALDMGTFYFQRQALTERGDQRLVFYDQPGHGRSSRLQSGDYDIAALGKSLAAVLDATVPDGHIILVGHSMGGMTIMAFAEQYPEWFGNRVTGVVLMSTSAGLVDKTKLGIQSLVARASAPFFPIWGGAARLGGGAIDKARYASSDLAWLLTRRYGFGDARPSPSLVTFVEQMNSKTSVETLTKYLHTLYTHNRFPALSALRGVPVLVLVGTKDYLTPVTHSEEILRHLPEAELVKIDNSGHVVMLEKADEVNAALIPFLEKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR68</accession>
    <name>U5VR68_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0301 protein AFR_05895</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0301 (AlgH) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358999.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05895"/>
    <dbReference type="EnsemblBacteria" id="AGZ39468">
        <property type="protein sequence ID" value="AGZ39468"/>
        <property type="gene ID" value="AFR_05895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1197"/>
    <dbReference type="eggNOG" id="COG1678">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057596_2_0_11"/>
    <dbReference type="OrthoDB" id="1736059at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00758">
        <property type="entry name" value="UPF0301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003774">
        <property type="entry name" value="UPF0301"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30327">
        <property type="entry name" value="PTHR30327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02622">
        <property type="entry name" value="DUF179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="19641" checksum="8C71FF143499F8C9" modified="2014-01-22" version="1">MESMTGQLLVATPTLKDPNFDRTVVLLVAHETGGALGVVLNRATEVPVADVLGNWGELAGDPAVLFEGGPVQPESAICLARTRPQVKKRVSGFHQVSGALGTVDLSADPDRLRDSVAGIRVFAGYSGWSPGQLEEEIAAGSWFVFDALPGDPFVSRPDDLWAMVLRRQGDILAAVAHFPPDVSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR99</accession>
    <name>U5VR99_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase_AAA_core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358868.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05240"/>
    <dbReference type="EnsemblBacteria" id="AGZ39337">
        <property type="protein sequence ID" value="AGZ39337"/>
        <property type="gene ID" value="AFR_05240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1060"/>
    <dbReference type="eggNOG" id="COG4637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032808_0_0_11"/>
    <dbReference type="OrthoDB" id="318943at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_AAA_core" evidence="1">
        <location>
            <begin position="239"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="594" mass="66551" checksum="3439A8D1FEE84B08" modified="2014-01-22" version="1">MQEEGRFLHGVSIGGYRSFSGEQQTLGGLSRINILAGQNNAGKSNIIRFLKGYLRPDAITPDELDLPAGRPRGSGMTMSLARPIRDDDLEAALEPYRQQGSIDAGLRALKKIFHASAMDRGYDDLVWFDYRLPDPSSGSWGTWRLGEQQIGQIVSAEFHGYGETQLRDATMSVFHRAGSSADNLRRLLELFSPLRHLPKFEVVEAFRQIRVDSATDTAQISTNGENLVRALQRLERPTIDRLSDEDRFEAINKFVQVVLDDESARLRIPHDAQIIQVRRASGTLPLSHLGTGVHQVVILAAAATLLEDHVVCIEEPEVHLHPLLQRKLLRYLATETTNQYVIATHSAHLLDSSDATVFHVQLTENGTEVQRAGTPQDLSNICHDLGYQPSDLLQANSVIWVEGPSDRIYLKHWISLVDPSLVEGIHYSIMFYGGRLLSHLSANDPEVDEFISLRRLNRHIAIMIDSDKRSSGASINATKRRIRDEFDDPTYPGFAWVTACREIENYVPRDVLREAIVATHPNAVPIHDGQAWSAPLELRGGRAHVDKVRVAREACLRIGPDNLRQFKLDQRLRQLITFISEANGATAPKPQNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRJ0</accession>
    <name>U5VRJ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-dependent transcriptional regulator, malt-like, luxr family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359084.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06320"/>
    <dbReference type="EnsemblBacteria" id="AGZ39553">
        <property type="protein sequence ID" value="AGZ39553"/>
        <property type="gene ID" value="AFR_06320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1282"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015308_1_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="797"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="863" mass="91102" checksum="11AAFA41A3D0B4F8" modified="2014-01-22" version="1">MEHWEFVGRADELARLTTVATIDAERGLILSGAAGIGKSRLLNEGVAELPPEHYAVHYASANIASSGLPFGGLAQILPPDPPAGLSPAGLLRWAVDGLHDAAGDRPIVLAIDDAHLLDAPSAALAHLLVREGATLLATLRIAEPVPPPISALWTEGLLSHAELAPLTEDESRALLTGLVGGPVEAGSAQRLARLGGGNPLLLRELVLAAVGGGELVRTYGFWRWTGRLTLAPSLADLVDTRIGGLTGGVRDVLELVSFGEPVGLSLLLRAAEPRDVETAEERGLIRVGVDERRRDVRLAHPLYGEVVRRRCPVTRSRRLLATLADLVEGAGAHRRDDLLRVAVWRLDSGTAQGGALLLDAARQAFARFDIDLARRLAAAAHDAGAGYSAAELLATVLLFADSPQEALAVLDATPDDSARRLTSRAAVEFFGLGQAAAADRLAAAELADPAEAARVRAFEAFIRLQLDELATARSLARGVLDEPASGVATKALARCVLAFLAAAGGDPRGSAELIAAVEADTAAWRRDTPTLQYALQMAEGTLVSVSMDLPAIDKILAAEFAHLAQAGGFGFGSGWVSLLQAQACLLRGQTEEALRATEQACAALAPARVYDGSAHFARANAAALRGEVTVAVDSLAVAEASAGTAVGLYYAWQEQARAWTLACRGELADAVRVLHRSATRLRADGFHAHELLALYDLVRLGRPELAADRMDALATTVGGRATPLLVRHARAAADEAAEDLFAVAREFAVLGYQLFAAEAAAGAVRIFRQARDPRALAASTLMADVLARCGVMRTPALMAVQPALTVRERQVAELAAEGVRSREIADRLYLSPRTVENHLQRVYTKLGVNGRVELAPALRLLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS31</accession>
    <name>U5VS31_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00935"/>
    <dbReference type="EnsemblBacteria" id="AGZ38476">
        <property type="protein sequence ID" value="AGZ38476"/>
        <property type="gene ID" value="AFR_00935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.186"/>
    <dbReference type="eggNOG" id="COG3087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1004061_0_0_11"/>
    <dbReference type="OrthoDB" id="1866549at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="270" mass="26424" checksum="A0719A0FDA018FCF" modified="2014-01-22" version="1">MLALAVSVAAGCSAPDGGGRDYNPAHQPGYVLPDDQAPGVPGPAPTWPGSGNGLGLPGIGQADPSEPDRIVRGGEVTAGKPASFALVNGADVVRVSVGDLGTDLFEVSTPAESKVVPKVDVDGSSVVAGLRDTGLGGPAVVTVVLSDDVRWGVRLAGGASDQAVDLTGGPGGDVDFAAGTSRAEVSLPAATGTQRVTLGGGASQLLVHLTGDAPVRVATKNGAGDVTIDGQNRTGVAGGTVFVAPGWDTASNRFDVDATSGVSSLTVARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSB5</accession>
    <name>U5VSB5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359233.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07065"/>
    <dbReference type="EnsemblBacteria" id="AGZ39702">
        <property type="protein sequence ID" value="AGZ39702"/>
        <property type="gene ID" value="AFR_07065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1441"/>
    <dbReference type="eggNOG" id="COG4914">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054928_1_0_11"/>
    <dbReference type="OrthoDB" id="1578466at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039498">
        <property type="entry name" value="NTP_transf_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14907">
        <property type="entry name" value="NTP_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="21627" checksum="F07076B6C2479EDF" modified="2014-01-22" version="1">MPHRVDEGLVNTLKRVASVLKQAEIPFALGGSFAVYAHGGHSSDHDVDFLIREQDKARALEELSAVGFEMEQPPEDWLVKVFDEGRMVDLIYRPVESPVTDATLRDTVMRPVEAINMPVLSATQLMIHKLLSYTQHYCDFATGLPVARSLREQIDWERVRAETAKSPYAEAFLVLLDRLDVVPLPADAQLTAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSE6</accession>
    <name>U5VSE6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359268.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07240"/>
    <dbReference type="EnsemblBacteria" id="AGZ39737">
        <property type="protein sequence ID" value="AGZ39737"/>
        <property type="gene ID" value="AFR_07240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1478"/>
    <dbReference type="eggNOG" id="COG3795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130902_2_2_11"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="16"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="111" mass="11789" checksum="00E83435D43FEA0C" modified="2014-01-22" version="1">MAQYAIFIYGGGPEHSTDAHDRYAQELITAGTMTLAYALEPIETSTSIRGDVVTDGPFIDAKEVVAGFYVIEAPDFDAALDIARRNPATQQGGGVEIRPIAGGFVRPQTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSG5</accession>
    <name>U5VSG5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39938">
        <property type="protein sequence ID" value="AGZ39938"/>
        <property type="gene ID" value="AFR_08245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08245"/>
    <dbReference type="HOGENOM" id="CLU_2630114_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="77" mass="8449" checksum="EDCC017F26EBA647" modified="2014-01-22" version="1">MDAVMQAIDVLGLDDPDNARTRLHLESTILQSATPSQDRARMVLRLATEVLATAEPTTLQMILDAAVREMAQRTGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSJ8</accession>
    <name>U5VSJ8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08395"/>
    <dbReference type="EnsemblBacteria" id="AGZ39968">
        <property type="protein sequence ID" value="AGZ39968"/>
        <property type="gene ID" value="AFR_08395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1709"/>
    <dbReference type="eggNOG" id="COG0433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011350_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="655"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1046" mass="112894" checksum="466874CCC4FEE063" modified="2014-01-22" version="1">MMNESERAALAALRFNWAPVPDDVWRPLPFHVDGLHPTVVREVLDGVADARASTGGSPIGLVLQGQRGSGKTHLLGWTRKQVQEQGGYFFLVGLLDAAGFWDSVLAAMLDGLSRPVPGSDETQLVVLLRRLSAMVGAPRTARRAVMGEKTLTRPALDAFIEGLGEFDRHVARASQETARALALSASDDVAHRDLAENYFISGEESEPGERHAWGMRRAPRHAQQIVQDLSWLLALTGPSVIAIDQVDTLIAQSALQDDPQLPRTALVDPEQALMLARIAEGLMSLRQVTRRTLTVVACIPTSWTIIKTSAADTVSDRFRLAPQLVKINDDDLGRAIIEKRFAVRYQEIGFTPPYPSWPVLPQAFAAAGDFTPRQLLIEIDKHIRTCIADDEVRELDHLGEPPATEPRAPRPAVVPVPVAEFDRLDARFAELRKNADVTAPLDRASEDAMVPPLLTAGLTAWIVERGDGGEAFSVDPPPSTNPPLHGGLRLTLNEHNEDQLHWFFRAISDGHHGVAALSRLRKACMAAGLDQRVAKRRLFLLRNGQWSGKKTLEAVAAFEAAGGRTLPFEAEDLRVLSALQIMLAEETIDLQSWLAERRPAHGLALLQQALSDVSEPAPEATPSEPLPMAEGSEPAFTIGYGYDSGTPARLPLEALRKHTTIFAGSGSGKTVLIRRIVEECALQGVSAIVLDPNNDLARLGEAWPEAPSSWRPGDEVQAADYLANTDVVVWTPRRASGRPLSFQPLPDFRSVRDDPDEFNEAVEAAVASLAPRAMVAGRAVKAHLGLAVLRKAVEHYGRRGGTRLKGLIDTLAGLPDGIIDLDGADKIGLSLAQTLTAAMVNDPLFGGDGTPMDPGMLLTPAPGKRARVSVISLVGLPSDDVRQSFVNQLQMALFAWIKKNPAGDRPLLGLLIMDEAQTFAPSGAMTACTQSTLALAAQARKYGLGLIFATQSPKGLHNRIPGNAATQLYGLLNSPIQIEAAREMARAKGGDVPDISRLTVGEFYLAAEGAAPQKIRTPLSLTNHPRSPLTVEQVLHIAGAHHAVPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSQ5</accession>
    <name>U5VSQ5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08645</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359549.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08645"/>
    <dbReference type="EnsemblBacteria" id="AGZ40018">
        <property type="protein sequence ID" value="AGZ40018"/>
        <property type="gene ID" value="AFR_08645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1759"/>
    <dbReference type="eggNOG" id="COG3973">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010312_3_1_11"/>
    <dbReference type="OrthoDB" id="114076at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027785">
        <property type="entry name" value="UvrD-like_helicase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13538">
        <property type="entry name" value="UvrD_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="6">
        <location>
            <begin position="182"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="203"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="705" mass="77791" checksum="A6BC2AC991E40B83" modified="2014-01-22" version="1">MSDATVLQQEIAVEQEHVDRVYARLADLRQDASRAEKEGYQLAGVGTFGALVERDAMVYHRARRRHALDAEYEGLVFGRLDLNTGAVHYVGRMGIRDENSQSLVVDWRAPAAAAFYRATPADPLGVIRRRMIQSARERVTNIEDDLLDPEAAPDDMRVVGDGALLASLSKATGTGMRDIVATIQREQDDAIRSPASGVTIVTGGPGTGKTAVALHRAAYLLYADRSRFAGGGILVVGPSGVFVNYIATVLPSLGEDTATLRSLGSLVVGYDAVRTDPTDVAALKGSLRIRRVLERASHDAVPGGPTELKLLYRGALLRLDNNELERVRRKALQRGARRNEVRGVGFDRLFDALWAQAKGSVRNLPEKREFEAELADRTDFRDFLRAWWPRLTPMRVLRWMAEPARLRAYANGLLSRDEIALLQGSFDDLAEHGPTIADVALLDELDELLGRPRQAQKKSGNPFHVRDGVQEVSTFSDRQAATRAQVVREEDYRDYAHVVVDEAQDVTPMQWRMIGRRGQYASWTVVGDPAQTAWSGDVDELDRSRDRALGSRRRNTYSLTTNYRNSSEIFAVAARVIRTIMPDLPLPSAVRSTGVEPVDLVVGAAQLPDAVRESAEKHLSDVDGTIGVITPVERRDEMAQWVAGLPERVQVVTALQAKGMEYDAVVLVEPAEIATDASGTRTLYVALSRATQRLTTVGTRDWTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSQ7</accession>
    <name>U5VSQ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08050"/>
    <dbReference type="EnsemblBacteria" id="AGZ39899">
        <property type="protein sequence ID" value="AGZ39899"/>
        <property type="gene ID" value="AFR_08050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1643"/>
    <dbReference type="eggNOG" id="COG4721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089225_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017195">
        <property type="entry name" value="ABC_thiamin-permease_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09819">
        <property type="entry name" value="ABC_cobalt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037394">
        <property type="entry name" value="ABC_thiamine-permease_YkoE_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="193" mass="20618" checksum="4DC35629633353E1" modified="2014-01-22" version="1">MTKYGWRTIDIVIASIIAVTFGVIFWAWNIVWGATETAFAFFPPAQTLLYGVWLIPAVLAGLIIRKPGAALFTETVAAVISALLGAKWGATVIPQGLVQGLGAELAFALLFYRSWKLPAALLAGLLTGVSAAVFDFFAWNAATDLWDYRIPYALLTIVSSTIVAGVGSWALTRALAPTGVLDRFAAGRDRALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSS8</accession>
    <name>U5VSS8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF397 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357634.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02010"/>
    <dbReference type="EnsemblBacteria" id="AGZ38691">
        <property type="protein sequence ID" value="AGZ38691"/>
        <property type="gene ID" value="AFR_02010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.407"/>
    <dbReference type="HOGENOM" id="CLU_131550_1_3_11"/>
    <dbReference type="OrthoDB" id="1996962at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007278">
        <property type="entry name" value="DUF397"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04149">
        <property type="entry name" value="DUF397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF397" evidence="1">
        <location>
            <begin position="8"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="66" mass="7278" checksum="440D0BF2B0E64ED1" modified="2014-01-22" version="1">MTKQTDDAGWRKSSLCGSGACVEVAKVGDEYLIRDSKDPGMTPLTFTQDEWLAFEAGVKAGEFRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSX5</accession>
    <name>U5VSX5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ADAM 12 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359448.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08140"/>
    <dbReference type="EnsemblBacteria" id="AGZ39917">
        <property type="protein sequence ID" value="AGZ39917"/>
        <property type="gene ID" value="AFR_08140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1661"/>
    <dbReference type="eggNOG" id="ENOG50318UG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061329_0_0_11"/>
    <dbReference type="OrthoDB" id="1098494at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="333" mass="35112" checksum="F5A931041D60F45C" modified="2014-01-22" version="1">MKPSKTAAPGRLLTQVAGERQTGALVVGGQPGGTVYLIEGQVTYAESAAAPGVGELLTASGRLAPRTWQAALDAGTSRAQVGQLLLDQGHLTQGELELCVLGATYDAAYFALAPATAPVDFLEGATHWLGPIVRADPAAVTREARRRARILDEIYPYPEIDTAAVIPVPRPPTERVTLTALQWELLVSADGQRTPADLAQLLGRAGFATIQELRKLAALGLVERPRPPADNPDFVRLPRARGTAVDVRRPQVAAPAVTGDRPAPASGDPPAAPEPGVVTTNGTDHHPAQPFQLARRKPGAKLPKDVAADTGQIHPGTDDVLLKRIRTALRALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT95</accession>
    <name>U5VT95_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative cyclase/dehydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359841.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09545"/>
    <dbReference type="EnsemblBacteria" id="AGZ40198">
        <property type="protein sequence ID" value="AGZ40198"/>
        <property type="gene ID" value="AFR_09545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1946"/>
    <dbReference type="eggNOG" id="COG2867">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128243_0_0_11"/>
    <dbReference type="OrthoDB" id="1691158at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="150" mass="16864" checksum="0C2A5C2EAF91E33C" modified="2014-01-22" version="1">MADTSTQSIQVHAPLAQVAAVICDFPRYPEWADAMKKVEVLEEYEDGYAAQVRFVIDAGPLADDYTLDYAYAEDLSRIEWHLVAPSKMQKTLNGSYDLADNSDGTTTVTYMLEVELSIGMLGMFRRKAEKMIMDTALKELKRRVESLAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTE4</accession>
    <name>U5VTE4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357955.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03605"/>
    <dbReference type="EnsemblBacteria" id="AGZ39010">
        <property type="protein sequence ID" value="AGZ39010"/>
        <property type="gene ID" value="AFR_03605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.725"/>
    <dbReference type="HOGENOM" id="CLU_2299635_0_0_11"/>
    <dbReference type="OrthoDB" id="2084415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022062">
        <property type="entry name" value="DUF3618"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12277">
        <property type="entry name" value="DUF3618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="10511" checksum="B252FA4438650AD3" modified="2014-01-22" version="1">MSSDVTVRLNGSTPGDPARLKAEIEQTRQELGETAAALAAKTDVKTRAAALAGRVRERVVVTADEIKQDPAQAVRRPVPVALLATVAAAVAVVLVRRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTK2</accession>
    <name>U5VTK2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359680.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09140"/>
    <dbReference type="EnsemblBacteria" id="AGZ40117">
        <property type="protein sequence ID" value="AGZ40117"/>
        <property type="gene ID" value="AFR_09140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1859"/>
    <dbReference type="eggNOG" id="ENOG502ZJZN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2165536_0_0_11"/>
    <dbReference type="OrthoDB" id="2191221at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022536">
        <property type="entry name" value="EspC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10824">
        <property type="entry name" value="T7SS_ESX_EspC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="110" mass="11966" checksum="B2EC2EE15CAC673E" modified="2014-01-22" version="1">MSFEVDPQAVRKYADELAAVRRAADTANAYVNKHGSFSTHESGLMGYISGAHADFVASLNTMLKHLMDLADSSEVALKQLAAKYEQTDESAAAKVDSTYPVVPRTSFKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTR4</accession>
    <name>U5VTR4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mycothiol-dependent maleylpyruvate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360225.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10520"/>
    <dbReference type="EnsemblBacteria" id="AGZ40393">
        <property type="protein sequence ID" value="AGZ40393"/>
        <property type="gene ID" value="AFR_10520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2144"/>
    <dbReference type="eggNOG" id="ENOG5031RF6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077935_0_0_11"/>
    <dbReference type="OrthoDB" id="1548598at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017517">
        <property type="entry name" value="CHP03083_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024344">
        <property type="entry name" value="MDMPI_metal-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11716">
        <property type="entry name" value="MDMPI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SSF55718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03083">
        <property type="entry name" value="TIGR03083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MDMPI_N" evidence="1">
        <location>
            <begin position="12"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="232" mass="24584" checksum="982BB31896A5F764" modified="2014-01-22" version="1">MTVDPLVLMTDVEQATERLIRTAEGLDDAAVKAPSALPGWTVGHVLTHVARNADAYTNLLTWARTGVETPAYASPAARTDGIDAGAERPLAEQIADIREAHERLADAGASMPAEAWTFFIPPTGASAAAIPWARLREVEVHHVDLGAGYSPEDWDDAFALRLLREIVTGASDLSLVLRPHGVDHPLTIGDADGAPTVAGPTRSIAAWLSGRGDGADLTVSPEGELPQPPRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU88</accession>
    <name>U5VU88_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 13" type="primary">rpsC</name>
        <name evidence="13" type="ORF">AFR_04535</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358178.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04535"/>
    <dbReference type="EnsemblBacteria" id="AGZ39196">
        <property type="protein sequence ID" value="AGZ39196"/>
        <property type="gene ID" value="AFR_04535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.916"/>
    <dbReference type="eggNOG" id="COG0092">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_B">
        <property type="entry name" value="Ribosomal_S3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005704">
        <property type="entry name" value="Ribosomal_S3_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_S3_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01009">
        <property type="entry name" value="rpsC_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="12">
        <location>
            <begin position="38"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="211"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ39196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="32022" checksum="DEB3E407A4542437" modified="2014-01-22" version="1">MGQKVHPTGFRLGISTDWRSRWFADKLYKDYIGEDVKIRRMMSKGLERAGISKVDIERTRDRVRVDIHTARPGIVIGRKGAEADRIRGELEKLTGKQVQLNILEVKSPESDAQLVAQGVAEQLSSRVSFRRAMRKAMQSAMKNPVCKGIRVQVSGRLGGAEMSRTEFYREGRVPLHTLRANIEYGFFEARTTFGRIGVKVWIYKGDAVPGREAAPEAPARPRRDRADRPERPRRGRSGSSGTTAGGTEAGRAARGRGGRPRSGGGCAHDRHRRCSGCGRNAAGGLTDADAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUF1</accession>
    <name>U5VUF1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative septum formation initiator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_05330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358886.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05330"/>
    <dbReference type="EnsemblBacteria" id="AGZ39355">
        <property type="protein sequence ID" value="AGZ39355"/>
        <property type="gene ID" value="AFR_05330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1079"/>
    <dbReference type="eggNOG" id="COG2919">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085342_0_3_11"/>
    <dbReference type="OrthoDB" id="1711819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007060">
        <property type="entry name" value="FtsL/DivIC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04977">
        <property type="entry name" value="DivIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="207" mass="22402" checksum="AA2C4D17A3A45FDC" modified="2014-01-22" version="1">MTQRRTPSGQGPARRPGGHAGRPGSRTAARPARTRDTAATRIEPRRTPATRTTSGIRSGNRPAAARRAAAAGATKRTVATRPKAFTGRATVLIIVLVVLALAYTYPVRVYLAQESQIAQMQAEQAAQQAKISGLTEEVEKWKDKEYVRIQARDRLFYVRPGEVPLLVLNDPEGAARAAGQTSASDAPDRWYDTLWGSVAAANAESRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUQ6</accession>
    <name>U5VUQ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12160"/>
    <dbReference type="EnsemblBacteria" id="AGZ40718">
        <property type="protein sequence ID" value="AGZ40718"/>
        <property type="gene ID" value="AFR_12160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2473"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_9_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13586">
        <property type="entry name" value="DDE_Tnp_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="26"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="14958" checksum="0928ED3FE946AA6B" modified="2014-01-22" version="1">MTLIDAVSVARPGGRGRPRKRLDHLTADKAYGSRANRRSLRSRRIPHTIPERDDVLAGRARRGARGGRPPIFNPDRYRDRNQIERAFNRLKQFRAVATRYDKLRDRYHATVTIASIIIWLRANPDRAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVH7</accession>
    <name>U5VVH7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06685"/>
    <dbReference type="EnsemblBacteria" id="AGZ39626">
        <property type="protein sequence ID" value="AGZ39626"/>
        <property type="gene ID" value="AFR_06685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1366"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_477058_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019405">
        <property type="entry name" value="Lactonase_7-beta_prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011045">
        <property type="entry name" value="N2O_reductase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011964">
        <property type="entry name" value="YVTN_b-propeller_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10282">
        <property type="entry name" value="Lactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50974">
        <property type="entry name" value="SSF50974"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="SSF51004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02276">
        <property type="entry name" value="beta_rpt_yvtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="571" mass="59231" checksum="9C5FA88832AF0A2D" modified="2014-01-22" version="1">MPPQVLAMIDSSVRAARLRGVDEAELLANSTDLAMAAAAWRVLRSLTDEDSRSVAAAATAALERSAVRFNPDRVDFGTVVRGTPELAAEVLIEGAPLAIATSTVTVAGPGLRARMDGRRLHIVWEPESDWLDGSVTVRGPTGWAEVRVTGLATAPPPSSRAEIEARLPSADGANDFAMSRMTVLSAPPPRRRSGATVLLAALTAGVVLGGGGVAVAMNRNEQAARQQVAALDGTSAPSTTGATPRTLAGPSVAAPKGTTVPRIPLARSVVSVAKPAVTGTIRVGKEPEGVAVSPNGRTIYVANQGERVLSVVDAVSRRVTSIKLRNTPRFVTTSRDGRLVFVSMYENDKKTGSGVAVVDAAQRKVVRYLITGDQPFTLAVGPDDRLWVPIHSSGRIEVFAAKDQHPDGRIAVKPNPHAVAFSANLMRAVTPNHESNAVSIIDMGSGKVLRSIGVSKAPHSVAVSPNGRTVLVAGYEADAANLIDLVTMRRSGPFKVGSKPQSVAFATDGAHAYVVNEGGGSVSVLNGRTGAVTATVRVGGSPRSVAVSPDGTLAYVSNGDDNTISVLRIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWR4</accession>
    <name>U5VWR4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359602.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08910"/>
    <dbReference type="EnsemblBacteria" id="AGZ40071">
        <property type="protein sequence ID" value="AGZ40071"/>
        <property type="gene ID" value="AFR_08910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1814"/>
    <dbReference type="eggNOG" id="ENOG50333N5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183045_1_0_11"/>
    <dbReference type="OrthoDB" id="2071349at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021741">
        <property type="entry name" value="DUF3311"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11755">
        <property type="entry name" value="DUF3311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="78" mass="9031" checksum="26DF64612C549F74" modified="2014-01-22" version="1">MSSPRSDRSPWNWLLIVPIVLPLLTPLYNFDSPRLWGFPAFYWLQFAFILVGVATTSIVYQMTRRPREAVKSEAATDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWV9</accession>
    <name>U5VWV9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361545.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16005"/>
    <dbReference type="EnsemblBacteria" id="AGZ41483">
        <property type="protein sequence ID" value="AGZ41483"/>
        <property type="gene ID" value="AFR_16005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3248"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030024_5_3_11"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="45"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="221" mass="24618" checksum="F0784D76A6F98163" modified="2014-01-22" version="1">MGEQVVNVCFHGIGVPARELEPGEDRYWVTEDQFHAVLDEIRTWPSVRISFDDGNASDARIGLPALVERGLTADFFVLAGRLGTAGSLDEDDVRELGKQGMGVGSHGMAHRSWRGMDEQTRRAELVEARDRIAGVSGSPVEAAACPLGRYDRELLNSMRSLGYTRVYTSDRRHARGGSWLQPRFSVRREDTPESLRAEVLHRPPLVRRAKLAAIGTVKRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX30</accession>
    <name>U5VX30_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lactamase_B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361488.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15735"/>
    <dbReference type="EnsemblBacteria" id="AGZ41429">
        <property type="protein sequence ID" value="AGZ41429"/>
        <property type="gene ID" value="AFR_15735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3195"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_3_3_11"/>
    <dbReference type="OrthoDB" id="1815576at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="34"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="30551" checksum="BD13CBA452502CB9" modified="2014-01-22" version="1">MSYSIWILEYGYVDRFPASNLFAAQPNEGYRRMPYCFGLVRSADRCILVDTGFADRAVHDRLTTKYGETRWRSPVEALAQVGVTAGQVDTILLTHNHFDHAGCVDVFPDAHVWIQRREIDSYVAASARASRFEFLTRSCQTDLPEQLGRRPSTLVEGVAEVAPGIELRPAHDTHTAGSQVVAVTNAADGTWVFAGDNVYSYENLDGLHGDGVLAPIAMTTGSAATWLDFADDLLTGVGGDSRRILPFHEAAVWDRFPSATGDDGLHVAEVSLAGGHPSVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX92</accession>
    <name>U5VX92_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361471.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15650"/>
    <dbReference type="EnsemblBacteria" id="AGZ41412">
        <property type="protein sequence ID" value="AGZ41412"/>
        <property type="gene ID" value="AFR_15650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3178"/>
    <dbReference type="eggNOG" id="COG0599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096750_0_0_11"/>
    <dbReference type="OrthoDB" id="1792151at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="38"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="161"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="28564" checksum="71F5C03C4A47EB60" modified="2014-01-22" version="1">MSLTPRQTEIKEEFIRVRGTWSDTWEGVLRLDPEFLLAYLNFSAVPWRFGTLDPKVKEFIYIAIDANATHMYLPGVRQHIKAAFALGATPQEIMEVLELSATLGIHAMNIGVPILVQVLEEKGLRTGPAPLTERQEQIKAEFTKTRGYWHTFWDEILELTPEMFEAYTEFSSVPWRTGTLEPKVKEFIYIAFDTAATHLYVKGLKLHIENAIDHGATAQEILEVMEIASVLGIHGVTAAAPILLEEIEAQRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXN2</accession>
    <name>U5VXN2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome P450</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361702.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16680"/>
    <dbReference type="EnsemblBacteria" id="AGZ41614">
        <property type="protein sequence ID" value="AGZ41614"/>
        <property type="gene ID" value="AFR_16680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3382"/>
    <dbReference type="eggNOG" id="COG2124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033716_0_2_11"/>
    <dbReference type="OrthoDB" id="816674at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002397">
        <property type="entry name" value="Cyt_P450_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00359">
        <property type="entry name" value="BP450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="SSF48264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="390" mass="42310" checksum="D6131704D94652E2" modified="2014-01-22" version="1">MEVDFADPGFVRDPYPVLGLLREQAPVSRHEPTGRWLAASHAAANAVLRDRRLGRFWQDREPAEVYEPFNLLHRHQMMENEPPVHTRLRSLVAKAFVRGHVERLRPAVAAEAGALLRAAGPEFDLLADVAEPLAVTVIAELLGVPVADRHRLRPWSAAIVRMYEVTRTPEVEAAALTAAAEFADYLRDLADRRRAEPRDDLVSHLVTVRDGTDKLSADELVASAILLLNAGHEASVNALGGGVVALLRHPDQLARLRADRGLIPAAGEEMIRYDPPLHMFVRIAAADVQVEGVTIPAGAEVAALLGAANRDPAVFAEPDTFDVGRDPNPHLGFGAGLHFCLGAPLARIELQAGLAALLDLPALALAAEPVQRPTFVLRAYTAVRLTTRQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXX3</accession>
    <name>U5VXX3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TROVE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_11280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ro 60 kDa family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360513.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11280"/>
    <dbReference type="EnsemblBacteria" id="AGZ40545">
        <property type="protein sequence ID" value="AGZ40545"/>
        <property type="gene ID" value="AFR_11280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2298"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_514729_0_0_11"/>
    <dbReference type="OrthoDB" id="912523at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040322">
        <property type="entry name" value="TROVE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008858">
        <property type="entry name" value="TROVE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037214">
        <property type="entry name" value="TROVE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14202">
        <property type="entry name" value="PTHR14202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05731">
        <property type="entry name" value="TROVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140864">
        <property type="entry name" value="SSF140864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50988">
        <property type="entry name" value="TROVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="TROVE" evidence="4">
        <location>
            <begin position="12"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="506" mass="55657" checksum="D2E2C4275E0E7325" modified="2014-01-22" version="1">MAKFNVKQRQTLVTAEGAPGFARAPRSELFLLAVSNLVGEDTFYEGAADRDARFRELVRTVAVADPEWFARFVRWLRLGANLRSASVVAALEGARAQVSEGIPGSRAVVDSALQRADEPGEALAYWLGRHGRSLPKPVKRGVADAAVRLYHERSLLKYDSEAASVRFGDVVDLTHPVAKDERQGELFRHALDRRHKRDNPVPASLSLLAARAELMALPVEQRRDTTDPEVLRAAGMTWEALAGWRQTAMDAAAWEGVIPSMGYFALLRNLRNFDAAGVSDAVAEQVAARLCDPDEVAKSRVLPMRFLSSYNAAPSLRWAYPLEKALQLALANVPALDGRTLILIDTSGSMNSDFSRDGSLRCWDAATVFGLALAARAKKPAVVSFSTTSKVFPSRRGESVLAGVRRFREDGYFLNAGTQTAQAVWKHYDGHTRVVVLTDEQAHWHGQEDVVAAVPREVPVYTWNLAGYRLGHTAPEDNRYTFGGLSDSAFSMIPLIEAGSREVWPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYT3</accession>
    <name>U5VYT3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_19205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19205"/>
    <dbReference type="EnsemblBacteria" id="AGZ42113">
        <property type="protein sequence ID" value="AGZ42113"/>
        <property type="gene ID" value="AFR_19205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3901"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="3">
        <location>
            <begin position="34"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="480" mass="51442" checksum="2314062642A8AE38" modified="2014-01-22" version="1">MLVGDMVLQKDQSGAVEVDQIDFLALHPAAAPAKGVTTWLVDELRAAITCGKLPAGSRLPATRVLAVELGLSRGVVVEVYQRLTDEGLLSGRTGAGTVVTALAPKPRQESRTRETSGMRLPLPPSPDIDLDLSPGVPDLSAFPRAAWLRAERQVLASASNADLGYGDPRGNPQLRAGLASWLGRTRGVQASADDLIVTSGVAQALALLSQTLRSHGVTDVAVEDPGSRGTRDELAHWGLRPVPVPVDDQGVRVDALDATGLETVLLTPAHQFPTGVVLSPARRRLLLDRPGLIIEDDYDAEHRYDRAPVAALQASAPDRVAYAGSVSKSLAPGMRLGWLIAPRRLHADLVTAKHASDLGNPALPQLIFARLLATGDYERHLRTVRRRQRTRRDALLLGLREHLPRARVEGVAAGLHLLITLPGRADDLALAARIEGAGVRVHPLSWHRQLPGPPGLVIGYAALPPDRLQEAARRIGRVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZN8</accession>
    <name>U5VZN8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362830.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20930"/>
    <dbReference type="EnsemblBacteria" id="AGZ42458">
        <property type="protein sequence ID" value="AGZ42458"/>
        <property type="gene ID" value="AFR_20930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4243"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035614_3_0_11"/>
    <dbReference type="OrthoDB" id="1439085at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="365" mass="38858" checksum="05D9D0DADFAD344B" modified="2014-01-22" version="1">MSLTHGTVAPGFEKVREVFAAEAARQDDVGAQLAVYRDGRQVVDLSTGDPDALTGVFSVTKGAAYLVVALLVQDGLIDVDRSVRHYWPQFTSDVTVRELLGHRAGLIGVDGGFGPREIADDRIIAERLAKQRPYWPRGSGYGYHALVIGALAGEIVRRVTGRSLQQTYEQRVRAPYDIDFHLGLPAELEPRYHPVRSSTVEELPPDAGSLAGIALNRHADPPTYLPAFPNSRTVRRLGQASAGGVANARGIARLYAAALSGVEGSPPLLRPRTITDFGTPGEPAPDLLTGVTGNQFGLGYEALTIRYPFLGPAAFGHFGAAGSLGFADPRSGVAYGYTRDRFAPPDGGAVENDQFAAAVIQAVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZT9</accession>
    <name>U5VZT9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_21155</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21155"/>
    <dbReference type="EnsemblBacteria" id="AGZ42503">
        <property type="protein sequence ID" value="AGZ42503"/>
        <property type="gene ID" value="AFR_21155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4288"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="16"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="139"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="139"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="239" mass="26397" checksum="370BE082FE64A696" modified="2014-01-22" version="1">MAGVDSLGQNPFVTARILVADDDPKHAQLIRLYLEREGHQVLTVGDGRAALEQARARKPDLVILDIMMPLVDGLDVCRILRAESSIAILLVTARSSENDMLLGLDIGADDYLAKPVSPRELTARVRALLRRIGATDTNAATLRVGALEVDAGRFEVRVGGEPVNLTAKEFGIVELLAREPGRVFTRGQIIDKTFGFEQEVSERTVDAHVVNLRRKIEENPAEPRYVQTVYGRGYRMAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZU0</accession>
    <name>U5VZU0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362659.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20075"/>
    <dbReference type="EnsemblBacteria" id="AGZ42287">
        <property type="protein sequence ID" value="AGZ42287"/>
        <property type="gene ID" value="AFR_20075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4072"/>
    <dbReference type="eggNOG" id="ENOG50322Q4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105743_0_0_11"/>
    <dbReference type="OrthoDB" id="1966197at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022062">
        <property type="entry name" value="DUF3618"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12277">
        <property type="entry name" value="DUF3618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="22253" checksum="F8E5AD9D32449283" modified="2014-01-22" version="1">MAEEPDRLRQDIENTRASLTRDVDLLAEKTSPAKVAQRRWTSVKEKVMGTSEHARHSAGGAASTVQDKASSAAGAVQDKASQLSDKAGEKAHDAADAVKSAPQAVAERTQGNPLAVGIIAFGVGLLASTLLPVTEAEKRAGEQIKERSGDLTDKAKEVASEFKDDLSGTVQQAVSEVKDTATDAAQTTKQQAQSSAQDVTQQSKQAVSEARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0R4</accession>
    <name>U5W0R4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ketopantoate reductase ApbA/PanE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_22245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363090.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22245"/>
    <dbReference type="EnsemblBacteria" id="AGZ42719">
        <property type="protein sequence ID" value="AGZ42719"/>
        <property type="gene ID" value="AFR_22245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4509"/>
    <dbReference type="eggNOG" id="COG1893">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2897">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_439891_0_0_11"/>
    <dbReference type="OrthoDB" id="920668at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="PTHR11364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="351"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="495"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="614" mass="63211" checksum="9A59735786B33464" modified="2014-01-22" version="1">MTRLVIVGAGAVGASVAAELHLAGGDTVLIARGAQLAALREHGLRYVRPGGTATVSVPVVGGPEEVDLRDGDVLVLATKSQDTEAALRQWAWQPVKRADGSSGVAATDLPVLVLQNGLANERAALRHFGQVLGAVIWIPAGHLTPGEVVSAGEPVPALVWLGEYPSGAGGHAGAVAAELTRAGLRVEVVDDIARWKAGKLLGNLVNGLDALYRASPLRERAVAALRAEAEAVFAASGIDPISPAADGFRSVAIPGHDRGGNSTRQSLARGATPEIDYLNGEIVLLGRLAGVGAPLNAAVQARVHRAAAEGTAAGSLGDDDLAATLPTLAEPTGDRAGVLITAAQLHRRLAEPGPPVLLDVRWALGDPDGEQHYRDAHLPGALYADLETDLAGPHEPGAGRHPLPSVARLQTAARRWGITRKRGVVVYDNSGGLAAARAWWLLRWAGVPDVRILDGGLAAWLAAGHGVATGQAQPQPPSDIVLTGGHLPTVTAEEAAASPVLLDARAGERYRGEVEPIDPRAGHIPGARSAPTTANLTGEIPLFRPTAELRERFQALGAGTGDVAVYCGSGVTAAHEIAALAVAGFDAALYPGSWSAWSSDPDRPIATGSEEIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5W117</accession>
    <name>U5W117_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_22845</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22845"/>
    <dbReference type="EnsemblBacteria" id="AGZ42839">
        <property type="protein sequence ID" value="AGZ42839"/>
        <property type="gene ID" value="AFR_22845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4630"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043648_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="7">
        <location>
            <begin position="7"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="8">
        <location>
            <begin position="107"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="31166" checksum="A49AD6BA8D130A98" modified="2014-01-22" version="1">MSLTEAFEAERRGLLAHAYRMLGAFHEAEDVVQDTYVRALRGWKTFEGRSSVRTWLYRIATNVCLTVLDGRGRRALLTGLVPDDLEVWVAADPGDLITDRESVRLAFVAGLQHLAPRQRAVLLLREVLAFSAAETGVALGMSVPAVKSALQRARSRLAEVAPTRDDVLDVSSPRARELLDGYMTAWEASDADAFRDLLCADASIEPVGAHSSYAGREACLAFATPSMGAAGDWRMAPTEANSQPAASVWFRGEPWGLAVLTIVQEGIRAVTLFPNQELIADNSARVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W143</accession>
    <name>U5W143_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_16985</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 130 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361825.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16985"/>
    <dbReference type="EnsemblBacteria" id="AGZ41675">
        <property type="protein sequence ID" value="AGZ41675"/>
        <property type="gene ID" value="AFR_16985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3447"/>
    <dbReference type="eggNOG" id="COG2152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_640768_0_0_11"/>
    <dbReference type="OrthoDB" id="1622507at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007184">
        <property type="entry name" value="Mannoside_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34106">
        <property type="entry name" value="PTHR34106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04041">
        <property type="entry name" value="Glyco_hydro_130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="496" mass="53539" checksum="D35444C7D4F169A4" modified="2014-01-22" version="1">MTLSPTPDLLAARQSVSLTPDPSRVIVKLFVPGEDAAVVRTRAQALIDRIAGLPGDEVDRLLGETVALFADRHRDLESTFLHHFELVRHRVEHAADLSPQRRLLVGAYFTNEYAVEAAALCNPSAVEHPDQTGLGAGQLRVALSVRQIGEGHLSSIGFATAVVGPGTELTVAGRTGPLVTGSRVPARHRRDLLAAGLADDGWDNEVAAALLGSLPEHFDDATFESVLGTLPDGLHSRENAQRTLEQLRRTNAAGYATAFPEDTHLHQRVLWPATPAESNGMEDARFVRFVGDDGVADYWATYTAYDGREIAARTISSPDLRQFHIEPMRGPGVRNKGVALFPRTVGGRRLALSRADGETIGLTELDDQARWRTPVPLHAPGRGWELIQTGNCGSPLETSAGWLVLTHGVGTMRRYAIGAILLDLDHPERVLAELPGPLLSPDATERDGYVPNVLYSCGGLIHDGVLWLPYGASDARIGFATVEVEALLAAMVRADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W148</accession>
    <name>U5W148_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361835.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17010"/>
    <dbReference type="EnsemblBacteria" id="AGZ41680">
        <property type="protein sequence ID" value="AGZ41680"/>
        <property type="gene ID" value="AFR_17010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3452"/>
    <dbReference type="HOGENOM" id="CLU_1056146_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665432" evidence="1">
        <location>
            <begin position="24"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="27083" checksum="AA22A6609704AF08" modified="2014-01-22" version="1">MHRSFVLATAALLAGTVPAVAGAPDTSPPRLVSVSFSRTSVAVSGLTLVPVRIQLRLTDTSGVAENATGMGPSPQITLGPVPGFQERLHPRLALTSGTARDGVWGATVNVPSTWDGVVRMTSVDAVDTAGNELSVRPADAALRVIGTHRPALTLTYTLLPGGGFRIHGRAYFTDTGRPLAGQPLAVAYDSGCDLEGGAADSIVTDARGRYEQRWDNGEPGAAGCAALTGAAAPGQFRTLLAYRLGVTRPGDSAATQEKLVGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W169</accession>
    <name>U5W169_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_22435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363143.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22435"/>
    <dbReference type="EnsemblBacteria" id="AGZ42757">
        <property type="protein sequence ID" value="AGZ42757"/>
        <property type="gene ID" value="AFR_22435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4548"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_11"/>
    <dbReference type="OrthoDB" id="1584669at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005713527" evidence="5">
        <location>
            <begin position="41"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="70"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="275" mass="30348" checksum="075E09E7D6BEC9FD" modified="2014-01-22" version="1">MTKRYPARYIVLAVLIVLLLLTVAPLLVVALNAVKSPADYASNGPLSIPKSLHWSGIVDFWQRVDFSRKLLNSFITASVVAVLGVLLSVLNAYALGIGRVKGRVWFLVFFLVANLLPQEVLVYPLYYLSKSLHLYDSLLSIIIIFTVIQSAFGTYLLSSVYAEFPTELLDAAAVDGAGRWRTLWRVVVPVSGPTLAVLFTFFFIWTWNEFFLPLVFLISNANQTVPVALGVLQGDRQMDATTTSASALIGILPAMLFFLIFQRTLTRGITAGAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W198</accession>
    <name>U5W198_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase S8 and S53 subtilisin kexin sedolisin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_16965</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16965"/>
    <dbReference type="EnsemblBacteria" id="AGZ41671">
        <property type="protein sequence ID" value="AGZ41671"/>
        <property type="gene ID" value="AFR_16965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3443"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_259580_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="SSF117281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="SSF49464"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665978" evidence="4">
        <location>
            <begin position="21"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="5">
        <location>
            <begin position="172"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1448" mass="148638" checksum="15195E15CF0BB31A" modified="2014-01-22" version="1">MAGILAAGLVAIGSATPASAAPAASKDDKIRPELTAQLKDKNAGDFWIRFGAKADLSAASAIKDWDKRGKAVADALRKTAADSQAGIKAELDGTGTKYQTFWASNAIKVTGGSQAMVQSFAAHAEVQGLYPTREYKVPEVTKGATEKTVNALEWGIANINADDVWDQYGVTGEGITVANIDTGVQFDHPALVKSYRGNKGDGTFDHNYNWFDVADSCNGAPCDTNNHGSHTMGTMAGSDGANQIGVAPGVKWIAANGCCPSDAALIESGQWMLEPTDLAGANPDASKRPNIINNSWGTTLPSNDPFMEDVTLAWTASGIFGTWSNGNSGSGCNTSGSPGSSVSNFSSGAYDINNNIASFSSRGAGQNGEIKPNISAPGVNVRSSVRGSGYASFNGTSMAAPHLAGAIALLWSAAPALVGDVAATRALLDNTAIDKADTQCGGTTDDNNVFGEGRLDALALLNAAPIGDTGTLNGTVTAEATGAPIPNATVTLTGAAARDLTTGADGKFSSLLPAGEYQVAVTAFGYAGKTASATVTTGATTTVNFALAAVPSVTVSGAVTDGSGHGWPLYAKVSVAGPSGVSDYTTPANGRYSFSLPAGATYAITVDPEYPGYETVTKDLVVGTRNVTNNVAVPVDLEACTTAPGYAYKSDGEYETFDGSTAPAGWSVVDNAGNGQVWKFTDSGARGNLTGGTGNFAIIDSDDYGAGGRQDTSLVSPVIDLSGVATPVVRFNQDYDWLGTDKADVDVTVDGGTTWTNVLRQSADARGPRTTEVPLPQAAGKSAVQVRFHYYDATYEWWWEVDDVLIGSARTCEPVDGGLVLGNVKDENTGTYVNGATVTSDDAPGEKATSMATPDDTGLADGFYWMFSSKTGSHKFTAKAGNYVSQTKTVDVEADWATQANFELAAGQLSVSPTALAGTARMPSGKVSKSFTVTNTGKAPVDVEFSERDNGFELLRADGSKLTKQQILNSEGAPEQRITAPTSFAAKPGKGIAAVAPAVQGPQAAPWSDISDYPATVMDNRVVNVDGKVYSIGGTDGTASTAKSFVFDPVAQTWAPIADLPGARSAMTVGVLDGKIIATGGWAAAGPDATTWSYDPAADAWTKKADNPAPRSAAGQAVVDGKLYAIGGCTTSSCLPMSNDVVRYDPDADAWETVADYPKALAFAACGGIDGAVYCTGGNDGSVSQKAGYTFDPSAGTWAAIADAPSDNWASSFAVANGTLLVVGGSQAGAISNAGFAYDPASNSWANLPNANTARYRGGAACGFYKVGGASGGFTATADSEALPGLEECAAGGADVSWLSIDKTEATLAPGAKVKVTVGMTANVDQPGTYSGGVAIKENTPYSVPPVDVTLTAQPPTTWGKLLGTVSGKTCSGTSAPLSGATVQVDSWAMSFTFTTDAAGKYAYWMDKRNNPLTMIVAKDGFKPQTRSTKINTSTPAVEDFALLPVRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1A4</accession>
    <name>U5W1A4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RhaSlike HTH-type transcriptional activator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17310"/>
    <dbReference type="EnsemblBacteria" id="AGZ41740">
        <property type="protein sequence ID" value="AGZ41740"/>
        <property type="gene ID" value="AFR_17310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3510"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047930_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035418">
        <property type="entry name" value="AraC-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14525">
        <property type="entry name" value="AraC_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="219"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="320" mass="35230" checksum="16B69B38F10E7644" modified="2014-01-22" version="1">MRTVHLNSSDLDQARGMLAEHFYPNVIDGLSPGALPAVDFEITRAGLFTIGDLRFGTDVRLRFGELGAYHVDLPSGGDLLWRQGRREPILATPRSAAVFSPDGDTTLDRWDAATRVLAIKIERPVLEAELERMLDAPVTTPLRLSPELDVSRGAGATWLRLAHLIAADAAQPEGLTGHPVLGRRLRESLVAGLLLAVPHNYRDRLERRRTTLATPRAVQRAIEVIRADPARPFTVADLAAAAGVSARSLQDSFQRHLSTSPMAYLRDVRLAWVHDELRRADPGRTTVTEVAYSCGFLHLGRFAGLYRSRYGVPPSATLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1A8</accession>
    <name>U5W1A8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17015"/>
    <dbReference type="EnsemblBacteria" id="AGZ41681">
        <property type="protein sequence ID" value="AGZ41681"/>
        <property type="gene ID" value="AFR_17015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3453"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="11"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="423" mass="44822" checksum="C16168290A71E229" modified="2014-01-22" version="1">MVTSPARIGSYRIERLLGIGSFATVWLGHDTVLGAHVAIKVLAENWSHDLRVRERFLDEARLLWRLDDERVVRVHALGELPDGRPYLVMGWAEGGSLRDRLAGDPIPAGPALTLLREICKGVAVLHDQGIVHRDLTPGNILFSGGRAVIADLGLAKALAAASGLTARAGTPGYMAPEQDDPLAIIDRRADVYGLGRLGLRLLPAPPPKIAEVLRTATARRPENRYPDASALGAALDRATIGRSRASPLALAAGRGVLGLASAVLLVALASDSIGDRLPPRPGTVVDAAGRLSLPVPAGWTARSGHWTRPGPVVVISPDPARWTTDTTVPGAFAWLWADAATTPSQFVAERPNQGCRPLPVRTLRAAGLDWYIAAFRACPDGRAEIVEAVGTRPGDPGLVYVQIAPPPDSPAFTDTLLAGVRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1H3</accession>
    <name>U5W1H3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363633.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43043">
        <property type="protein sequence ID" value="AGZ43043"/>
        <property type="gene ID" value="AFR_23875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4836"/>
    <dbReference type="eggNOG" id="ENOG502ZN96">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1923151_0_0_11"/>
    <dbReference type="OrthoDB" id="2084577at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666277" evidence="2">
        <location>
            <begin position="29"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="14208" checksum="5CBAD531872D44B5" modified="2014-01-22" version="1">MRLSLSGSIVVILLLAGGCSSTDKPPAAAPSVSGSPSATTSAVPSPAPSSKAPFAAADGTNLKACADGACEVIVRSGDSVPAAGGLGKVKISVRGDEVTISKKTADGFTSTLTGTPGLVQQINKQLFLIVAVEDGRAVLRMALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1S4</accession>
    <name>U5W1S4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_17990</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362224.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17990"/>
    <dbReference type="EnsemblBacteria" id="AGZ41876">
        <property type="protein sequence ID" value="AGZ41876"/>
        <property type="gene ID" value="AFR_17990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3650"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_0_11"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="82"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="308" mass="32844" checksum="BF47F3BEEE040F6E" modified="2014-01-22" version="1">MTALAPPPVRVADRSTSTGGRSKSVVWMVLPALVMFAAFGIIPLLGVLGLSFTQWDGIGAINPAGADNWFSVLSEPGLLHALWVTFLIMALSWVFQTPVSLLIGVFLASRKRYRGFLAVLYFIPLLLSSAAIAITFSALLNPNFGLGAGLGVEFLNQDWLGQSNLAIATLIFVVSWQFIPFHSLIYQGGVRQIPTSMYEAAQIDGAGRVRTFFSITLPQLKYTIITSSTLMVVGSLTFFDLIFVLTAGGPGDATRVLALEMYQTGFRANLMGPASVIAVILVLVGLGLALLLRRLGGGSASDSQLEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1U7</accession>
    <name>U5W1U7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4037 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363788.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24660"/>
    <dbReference type="EnsemblBacteria" id="AGZ43198">
        <property type="protein sequence ID" value="AGZ43198"/>
        <property type="gene ID" value="AFR_24660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4996"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056336_0_0_11"/>
    <dbReference type="OrthoDB" id="681174at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025117">
        <property type="entry name" value="DUF4037"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13228">
        <property type="entry name" value="DUF4037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4037" evidence="1">
        <location>
            <begin position="96"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="337" mass="36426" checksum="F4AA0BB2FEADFC1F" modified="2014-01-22" version="1">MHGIALSRRLYEEAVRPSLGGRPHAAALLGAGSEVLGFDDEVSTDHDFDPRVQIFLPRGVDPPAPAPPGAEFTSVRAFFGERLGFDPAAGMRPADWLLTPTQVLATLTAGAVFHDPVGEVARYQSLLRWYPDDVWRYALAASWLKVAQEEAFPERTGGVGDDLGSRLVTARLTRELVRLAFLIERRWAPYTKWLGSAFARLDLAAPVGPLLTAALSAPGWREREDALVRAASLLAAATNDLNLAEPIDPSPRQYYTRDVRVIGADRLVVALTTAITDPDLRALLDRLGGRRDGPIGRLPGTIDQAVDSTDILGDPPRCHAAAPMLGLTADSARHPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1U8</accession>
    <name>U5W1U8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleoside-diphosphate-sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18115</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362274.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18115"/>
    <dbReference type="EnsemblBacteria" id="AGZ41901">
        <property type="protein sequence ID" value="AGZ41901"/>
        <property type="gene ID" value="AFR_18115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3677"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599196_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="2">
        <location>
            <begin position="7"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="17345" checksum="D7DF17C7C78A6211" modified="2014-01-22" version="1">MDIFVIGATGFVGGAAARHLAARGHSVSGLARSDAAARALSEQGIVAVRGDLEEGRAAVVASVADAVIVAAQLDPTVEQVLVDELLDALAGTGRTLLLTSGTGAFLQRTGGAWSEDVYAEDDPFEVEPLARQRFAVEQAVRRAGRHPRPRRSGTQPEPVRGEPRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1V4</accession>
    <name>U5W1V4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363793.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24685"/>
    <dbReference type="EnsemblBacteria" id="AGZ43203">
        <property type="protein sequence ID" value="AGZ43203"/>
        <property type="gene ID" value="AFR_24685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5001"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070586_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025847">
        <property type="entry name" value="MEDS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14417">
        <property type="entry name" value="MEDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="197"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="30692" checksum="B4B865249EF3886B" modified="2014-01-22" version="1">MADATMAGRLRPGDHVCWTYADDAERRRITAACVRAGLRDRHKVLWCTLDLEPAAALAELEDAGIRTSAAVRAGRLTVMTAASSYLVSGSFDAEATCRIWPDEFRRARREGYAGMRVLGDMSWAGGTTPGADRVGWYELQVNHILADGYALGICLYDRRRFTGDTLTTLCRAHPVTLSERSSAREPLLRIIRTRAGLRLSGEADVSNRDALATMLGAAIDEPADRPVVALDLSELCFADAATGKLIVDAGRRAGGRLRITAARPPVHRLLAQFGGEAVPGLLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2D3</accession>
    <name>U5W2D3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spermidine/putrescine import ATP-binding protein potA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_19315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362507.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19315"/>
    <dbReference type="EnsemblBacteria" id="AGZ42135">
        <property type="protein sequence ID" value="AGZ42135"/>
        <property type="gene ID" value="AFR_19315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19315"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="29078" checksum="0E282AD22CBC10DB" modified="2014-01-22" version="1">MLEVKGLRKVYRSGTREVEALRELTFTVAAGELVCLVGPSGCGKTTLLRCIAGLLEPTSGQVEVGDKAGLAIVFQEYGRSLFPWLTVRDNVDLPLRQKKVPRARRRQLVDEALTAVGLAGTESAYPWQLSGGMQQRVAIARAVAYEPSVLLMDEPFAAVDAQTRADLEDLVRSLWQRLGVTIMFVTHDIDEAVYLGQRVLMLSASPTVIQDELTVDLPAERDQLHTRADPRFTALRTRVYEQIQLAKVRTGTASGAAGELSSADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2I5</accession>
    <name>U5W2I5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_25400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363955.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25400"/>
    <dbReference type="EnsemblBacteria" id="AGZ43344">
        <property type="protein sequence ID" value="AGZ43344"/>
        <property type="gene ID" value="AFR_25400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5146"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_11"/>
    <dbReference type="OrthoDB" id="1510342at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="84"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="30964" checksum="3D13ABB49C07B66F" modified="2014-01-22" version="1">MTTALHRAAPPDAVPKKKDRGGPWLSLLAWVVGILFVLPVVWMVLTSFHTEADAATNPPAVFAPLTLEGYREFFSGGATPWPPLLNSLTASLVSTALVLLLSFPAAYALSIKPVKKWTDVLFFFLSTKMLPVVAGLLPLYLFAQNTGLLDNIWLLIVFYTSMNLPIAVWMMRSFLSEVPVEMLEAASMDGASLSKTLRSVIAPVVMPGIAATALICFIFSWNELLFARVLTATVAETAPVFLTSFVTSQGLFLAQLCAAAFVVSLPVLVAGFAAQDKLVQGLSLGSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2N6</accession>
    <name>U5W2N6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative PfkB-family carbohydrate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_19865</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362617.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19865"/>
    <dbReference type="EnsemblBacteria" id="AGZ42245">
        <property type="protein sequence ID" value="AGZ42245"/>
        <property type="gene ID" value="AFR_19865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4031"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_10_2_11"/>
    <dbReference type="OrthoDB" id="1030724at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="4">
        <location>
            <begin position="2"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="PfkB" evidence="4">
        <location>
            <begin position="286"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="189"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="379" mass="37180" checksum="3251A33F8F1BF92E" modified="2014-01-22" version="1">MHVVCVGLATVDLVQRVDRMPGPDEKAEALSASVAAGGPATNAAVTAAALGATVTLVTAVGSHPLAALIRADLAECGVTLLDATPEATTPPPVSAVTVLAATGQRTIVSRNARGSEVAAPAGLASVLASADVVLVDGHHPALARAAAASGRLLVLDAGSWRPIFAELLPLADVVAASAAFRLPTPGLSTAGLPTPGPTDATHPAADFATPDANPPAAAYAAPDADPPAADLAAPNANPPGADLAAPNANPPAAGSAALGVGPPTADLAQPGPTDVVPPPGDHEGISAREALRVAGVRRGAITRGPDPIIWWSGDENGELAVPAVPAVDTAGAGDVFHGALAVALGRGLTLVPALRFATEIAGIRVRHAGPRSWLAALRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2T4</accession>
    <name>U5W2T4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25875"/>
    <dbReference type="EnsemblBacteria" id="AGZ43439">
        <property type="protein sequence ID" value="AGZ43439"/>
        <property type="gene ID" value="AFR_25875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5245"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="30624" checksum="02E1B4AC9F18036D" modified="2014-01-22" version="1">MPLPPQVTDLTAFDLLLSVARLGSVGRAAAAHHISQPAASARLRELERRLGLTLLHRSPRGSTLTGTGALVADWARAAVDAADALATGVAALRTTHESRLAVAASQTVAEYLLPAWLVALRSGHPGLSVTLRAANSAEVAAAVLAGEVDLGFVEGPDVPAGLHAEAVAADRLAVVVGTGHPWAKRRRGVTPDELAATPLVSREAGSGTRRAFEEALQPLVAAAPLLELSSTTAIKHAIAAGSGAAVLSSLAVTAELAAGTLVAVPSAMPLQRQLRAVWPAGAQLTGPARDLYAIARRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2U0</accession>
    <name>U5W2U0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SMP-30/gluconolaconase/LRE-like region-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362603.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19795"/>
    <dbReference type="EnsemblBacteria" id="AGZ42231">
        <property type="protein sequence ID" value="AGZ42231"/>
        <property type="gene ID" value="AFR_19795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4017"/>
    <dbReference type="eggNOG" id="COG3386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036110_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGL" evidence="1">
        <location>
            <begin position="10"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="27390" checksum="53072CCCA38C5AEF" modified="2014-01-22" version="1">MRVVLDGLVMGESVRWHDGRLWFCDWGAGQIVMLDAGLPSVVARVDDFPSCIDWLPDGRLVVITGRGPLLRQEADGSLAVFTTLTSPFLFNDIAVDGRGRIFVNNIGYDFPGGSPAPGTVSVVTPDGVAREVAGGLEFPNGMAVDGETLIVAESHAGRLTAFDIAPDGSLSGRRVWAPLGEGAAPDGIFAAGDGTVWYADVPNQQCVLVREGGQVLRTIPLDRGGFDCAVGDGVLYAATAVYPSAEPSGQLVAVDLADYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W301</accession>
    <name>U5W301_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hybrid signal transduction histidine kinase K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26555"/>
    <dbReference type="EnsemblBacteria" id="AGZ43573">
        <property type="protein sequence ID" value="AGZ43573"/>
        <property type="gene ID" value="AFR_26555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5383"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025828">
        <property type="entry name" value="Put_sensor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13796">
        <property type="entry name" value="Sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="2">
        <location>
            <begin position="352"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="442" mass="45881" checksum="3D710D420555DDED" modified="2014-01-22" version="1">MTITAVAPFTRRHLSGLAFCVAALPFALVSPPVLVVLGTDLIWWAADGGRGNPSPAQIAGAGAGAGLLLVLLVSSPAARRLGALQIRLADRLLGTRVALPPPVRRAWPGPRDGTGWRVVAYLLVKLPVGLLQLYAVALWAGGLVNLAYPLIWGAFRNHPPGTRLSPLPVFTPFGWFGDGTFHVTTLPGTFAAAAAGAAMLLVAPWVTRAVTAVDAFLIRGLLGPGRLAQRVHDLETSRALAVDDSAALLRRLERNLHDGAQIRLATVALNLGLARKKLGADGEVEDLAAVRELVGTALLGAKEALGELRDLARGLHPPVLDNGLPDALASLAATSAVPVELEVSIPVRPTPAIETIVYFCAAELLANAAKHSRATRLAVRAAGQRDVLRLSVADDGTGGADPGRGTGLTGLAQRVAVVDGSLRIASPPGGPTRVTVELPLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W318</accession>
    <name>U5W318_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative LacI-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_26310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364309.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26310"/>
    <dbReference type="EnsemblBacteria" id="AGZ43524">
        <property type="protein sequence ID" value="AGZ43524"/>
        <property type="gene ID" value="AFR_26310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5333"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="OrthoDB" id="664909at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="35183" checksum="43548DED2AAAE333" modified="2014-01-22" version="1">MSAGLREVAARAGVSVRTVSNVVSGFALVAPETRARVQRAIDELQYRPNAAARHLRGGRSGLVALAIPEIASPYFGELAGLLVREAEQHGWMLLVQQTDGDPDQERRLLEGVRGQVIDGLILSPWALSPEDLRRGPGGVPLVLLGEQDADGLLDHVAVDSVAAAYEATTHLIGIGRRRIAAIGLQPHLENGTAARRLDGYRQALHAAGLEPDPALEVPVQRLHRADGAAGLRSLLDADASFDAVFCFSDQLALGAMQAAQQHGLRIPADLAVVGFDDVEDGLYATPSLTTVSPDKAAIAREALTCLADRTPDKPPRRIVAAHRLVIRDSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W320</accession>
    <name>U5W320_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo-keto reductase-like family 1 member C1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364444.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26655"/>
    <dbReference type="EnsemblBacteria" id="AGZ43593">
        <property type="protein sequence ID" value="AGZ43593"/>
        <property type="gene ID" value="AFR_26655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5402"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_11"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="16"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="35965" checksum="DF834578744BAF7D" modified="2014-01-22" version="1">MHQRPLGRSGLAVSRLALGTMTWGRDTDPDDAAAQLKLYLEAGGTLLDTADVYGDGDAENVIGSLLDHLVPRDEVLIATKAGLGQHGFRRRDSSRGHLLRTLDASLRRLGTDYVDLWQVHGYDPQTPLEETLSALDHAVNSGRVRYVGVSNFAGWQTARAAAWQQAFPARAPIVATQVEYSLLERGIEREVLPAAAALGIGVLPWSPLGRGVLTGKYRNGRPLDSRAATEHLAPFVQTYLEPRSSSIVEALVIAAGGLGVSPLEVALAWIRDRPGVVAPILGARTAGQLQGALLSDELTLPYEIAAALDDVSAITVGYPEREGLTGPGTGDTPDLGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W350</accession>
    <name>U5W350_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364522.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26860"/>
    <dbReference type="EnsemblBacteria" id="AGZ43633">
        <property type="protein sequence ID" value="AGZ43633"/>
        <property type="gene ID" value="AFR_26860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5443"/>
    <dbReference type="HOGENOM" id="CLU_2021759_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="12095" checksum="A1B7DD94484BFDCC" modified="2014-01-22" version="1">MTISAPFKLGLAAFATAGLLDVLQLFALNSSAPAPVGVILLAAGLGLLSMIGAAVAWSGSRAGLLTAVAARVVATALGLPAFGLGAPLWVCVIIALSIVLSVVGLFLTAGTLRRRSVQAARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W393</accession>
    <name>U5W393_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560016.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27085"/>
    <dbReference type="EnsemblBacteria" id="AGZ43678">
        <property type="protein sequence ID" value="AGZ43678"/>
        <property type="gene ID" value="AFR_27085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5489"/>
    <dbReference type="eggNOG" id="ENOG5032R8Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1509722_0_0_11"/>
    <dbReference type="OrthoDB" id="1404858at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20169" checksum="FD95138C5737A93B" modified="2014-01-22" version="1">MAGPSTALSPVIAASTHWLIRAYPSSGADRAADTLAELQARQAVTVAAWLRYPTPVDAELVAMAGPGGSAVLDWKAGSEPDDEMAEDEGWRTWVDEVVVSWAACLLADPLLAARAVDSVAAVVPINDDRPRPRPRRAGDLPTEFRRLTAPDSRDLEAAALLRHPDLLETVADLHRDDLLYLLPSSMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3G1</accession>
    <name>U5W3G1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glucose-1-phosphate adenylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ADP-glucose pyrophosphorylase</fullName>
            <shortName evidence="9">ADPGlc PPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">ADP-glucose synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">glgC</name>
        <name evidence="11" type="ORF">AFR_21020</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the biosynthesis of ADP-glucose, a building block required for the elongation reactions to produce glycogen. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-glucose 1-phosphate + ATP + H(+) = ADP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.27" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362848.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21020"/>
    <dbReference type="EnsemblBacteria" id="AGZ42476">
        <property type="protein sequence ID" value="AGZ42476"/>
        <property type="gene ID" value="AFR_21020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4261"/>
    <dbReference type="eggNOG" id="COG0448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_14_1_11"/>
    <dbReference type="OrthoDB" id="1557977at2"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008878">
        <property type="term" value="F:glucose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00624">
        <property type="entry name" value="GlgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005836">
        <property type="entry name" value="ADP_Glu_pyroP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023049">
        <property type="entry name" value="GlgC_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="PTHR43523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02091">
        <property type="entry name" value="glgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00809">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00810">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 9" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="10">
        <location>
            <begin position="7"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha-D-glucose 1-phosphate binding" evidence="9">
        <location>
            <begin position="178"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
        </ligand>
    </feature>
    <feature type="site" description="Could play a key role in the communication between the regulatory and the substrate sites" evidence="9">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="site" description="Could play a key role in the communication between the regulatory and the substrate sites" evidence="9">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ42476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="410" mass="45132" checksum="EEE184C1DB91ABFF" modified="2014-01-22" version="1">MAVKVLAIVLAGGEGKRLMPLTADRAKPGVPFGGIYRMIDFVLSNLANGGFLKIVVLTQYKSHSLDRHITKTWRMSTLLGNYVTPVPAQQRLGPRWFAGSADAIYQSLNLINDESPDYVIVFGADHIYRMDPKQMVDDHIASGAAVTVAGIRQPKSMSDQFGVIDVGPDGRRISAFREKPVDVEGLPDAPDEIYASMGNYVFTTRALCEAVTADAQNPDSKHDMGGNIIPMLVERGEANVYDFRDNEVQGSTERDRGYWRDVGTLDSFYDAHMDLIATLPVFNLYNMDWPIFTNYGSWPPAKFVHGYDDRQGRAVDSMISPGVVVSGSLVERSVVSPNVRVNSWAHVEGSVLMEGVQVGRRAVVRNAIIDKNVIIPEGAQIGVDLDRDRKRYTVSDSGIVVVGKGQRVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3G9</accession>
    <name>U5W3G9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_26425</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364354.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26425"/>
    <dbReference type="EnsemblBacteria" id="AGZ43547">
        <property type="protein sequence ID" value="AGZ43547"/>
        <property type="gene ID" value="AFR_26425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5356"/>
    <dbReference type="eggNOG" id="COG1141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_6_4_11"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001080">
        <property type="entry name" value="3Fe4S_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00352">
        <property type="entry name" value="3FE4SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="10473" checksum="77DDDA99713C1379" modified="2014-01-22" version="1">MSTQTETGELEVWVDQDLCTGDGLCVQYAPEVFEFDIDGLAYVKSSTGELLQNPGVRTDVPARLRLEVIDAAKECPGDCIHVVRSSDDVEVAGPDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3K3</accession>
    <name>U5W3K3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IucA/IucC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362893.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21245"/>
    <dbReference type="EnsemblBacteria" id="AGZ42521">
        <property type="protein sequence ID" value="AGZ42521"/>
        <property type="gene ID" value="AFR_21245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4308"/>
    <dbReference type="eggNOG" id="COG4264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018524_3_0_11"/>
    <dbReference type="OrthoDB" id="856726at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007310">
        <property type="entry name" value="Aerobactin_biosyn_IucA/IucC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022770">
        <property type="entry name" value="FhuF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037455">
        <property type="entry name" value="LucA/IucC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34384">
        <property type="entry name" value="PTHR34384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06276">
        <property type="entry name" value="FhuF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04183">
        <property type="entry name" value="IucA_IucC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665661" evidence="2">
        <location>
            <begin position="20"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="IucA_IucC" evidence="3">
        <location>
            <begin position="127"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="FhuF" evidence="4">
        <location>
            <begin position="369"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="539" mass="56682" checksum="5EFE73A7399046DD" modified="2014-01-22" version="1">MTRILAARRLAITASHAGAALAVHAPELADGFTAALPEAAEIVGRRLRGALVREGLLPTGRGDRVHAFHRVEYDRAGAADPADLLPDGLPGNAAGFAAELRNGVVNLAIAMARQEKVTGADPDHAVIAGERLAVAGHNLHPCGRTRLGWDTSDVLAHDLESGHTRLGFVAVRDDAHLGDDVGAALRAVYPQVPEPPPGFRLQPVHAWQRDSVLGERYRELISDGVLRILDAELDTVPTAALRTLLLPVAADGSRRYVKVSLDIQVTSTRRSISVASTRNGPAVSAMLHRLVAEDPAADRLLLMAETAGAAVPVGSGRDMSAIVRDGLTGRLEAGEQAVAGSALPFAGAGLVADFAATTGRRDNTTAAAAFLDAYSRLVLPPLLRLTVRGVALEAHLQNCIPTFKKGVPHRLAVRDFAGLRLHPGRLAAAGHEVALWPGSVVGTPDAAVLRAKIGYTAFQAHLGELIIRLGESYGLDEDAAWRLVRAVVDETYAGLGPVAAEDHAAFTAPTVPHKALVRMRLAGAGDEYLPVENPLHAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3N0</accession>
    <name>U5W3N0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364500.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26800"/>
    <dbReference type="EnsemblBacteria" id="AGZ43622">
        <property type="protein sequence ID" value="AGZ43622"/>
        <property type="gene ID" value="AFR_26800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5433"/>
    <dbReference type="eggNOG" id="COG3920">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087828_0_0_11"/>
    <dbReference type="OrthoDB" id="1832160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="14"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="26106" checksum="870ADACD63EEAC47" modified="2014-01-22" version="1">MPVDCTISDDGSRLLATLSGTLEMADVSRLHERLLKCLAEQPDALLVELSGLGVADPLALSVFVAVRRQAARWPGIPMVLCAPSALARLALDSAAYRSLPVLATLAEARVQSGIERESLSTITDELLPITGASRQGRDVATDACARWGLPDLIGPASLITSELVSNVIDHAGTMMTLRLSLRPRFLQIAVRDGSTAEPVRLPPQGATAMRGRGLALIEGSAQSWGWLPCEGGKVVWASLSIDPERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4F5</accession>
    <name>U5W4F5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363275.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22680"/>
    <dbReference type="EnsemblBacteria" id="AGZ42806">
        <property type="protein sequence ID" value="AGZ42806"/>
        <property type="gene ID" value="AFR_22680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4597"/>
    <dbReference type="HOGENOM" id="CLU_2230674_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022536">
        <property type="entry name" value="EspC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10824">
        <property type="entry name" value="T7SS_ESX_EspC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="105" mass="10738" checksum="52D5C7CA22A36DE2" modified="2014-01-22" version="1">MYEPVQEVDIPGLTRVGSTAEQASVTWGKAHSAHAGDLTPGSSFDGWASGPALGAAEQGWSAFMTRLKGELHTYAAGLTQSAKDYQAADQAAAQRAGGAYGGGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Q1</accession>
    <name>U5W4Q1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha-galactosidase</fullName>
            <ecNumber evidence="5">3.2.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Melibiase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_29165</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29165"/>
    <dbReference type="EnsemblBacteria" id="AGZ44094">
        <property type="protein sequence ID" value="AGZ44094"/>
        <property type="gene ID" value="AFR_29165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5908"/>
    <dbReference type="eggNOG" id="COG3345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013093_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052692">
        <property type="term" value="F:raffinose alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000111">
        <property type="entry name" value="Glyco_hydro_27/36_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="PTHR11452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00512">
        <property type="entry name" value="ALPHA_GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665687" description="Alpha-galactosidase" evidence="6">
        <location>
            <begin position="25"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="7">
        <location>
            <begin position="408"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="538" mass="57102" checksum="D4E60908825080E5" modified="2014-01-22" version="1">MLAAGLLLLSGTAFVATGASPAQALDNGVSRTPPMGWNSWNTFFCNINESLIRGMADTMVSSGMRDAGYQYVVVDDCWMNPNRDSSGNLQADPTRFPSGMKALGDYIHARGLKFGIYQAPLDKTCAQYFGSYPGATGSQGREAQDARSFAAWGVDYLKYDWCSPSGSINDQVTTFAKMRDALAATGRPILYSINSNSIHSKTGPQRNWGDVANIWRTTEDIQLVWNTDQVNDYPMGVQNIINVNVPLAGYAKPGGFNDPDMMEVGRGTLTDTEQRSHFAMWAVMAAPLIAGNDLRNMSAATQTILKNPRLIAINQDSLGLQGVQVAGDSNQRVLAKRLANGDVAVALFNQSGATRTISTTAAAIGKSGSSFSLAEAWNGGTTTTSGAISASVPAHGTVVYRVSGGQTGEPPVPTSTSLVGTQSGRCLDLPNSSTTNGTQPVIWDCNGATNQKWTVSGQTLQALGKCLDAPVGATAGAKAQIWDCNGGTNQQWTFQSNGTVRSNQSGLCLDVNNNQTANGTLTLLWTCTGAANQQWTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W527</accession>
    <name>U5W527_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LuxR family two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_30315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30315"/>
    <dbReference type="EnsemblBacteria" id="AGZ44323">
        <property type="protein sequence ID" value="AGZ44323"/>
        <property type="gene ID" value="AFR_30315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6138"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="146"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="23063" checksum="4B325AC536BD7CE2" modified="2014-01-22" version="1">MSRRLIRVLVVDDEPMVCAHLRTILGSVPDIEVVDRAHDGAAAIISVLRHRPDVVLMDLRMPGLDGLTAIERLAALPAPPIVVALTTFDADHFVTRALRAGAVGFLLKSTPPEDPIDLVRVAAEGHTVLSPVVTRRLLGTPAADRRLALVGELTERETQVLACLGDGLPNAQIGARLHLAEATVKGYVSRLLVKLQCANRTQAGLLAQQAGLAGGAGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W596</accession>
    <name>U5W596_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferredoxin fas2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560656.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30295"/>
    <dbReference type="EnsemblBacteria" id="AGZ44319">
        <property type="protein sequence ID" value="AGZ44319"/>
        <property type="gene ID" value="AFR_30295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6134"/>
    <dbReference type="eggNOG" id="COG1141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_6_0_11"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="65" mass="6830" checksum="8F1B8648DDFBC7E5" modified="2014-01-22" version="1">MTRIVADTTRCVGAGQCVLTDPATFAQDSTQGTVVLLRDDLSDEALATARQALYLCPSRALSLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W598</accession>
    <name>U5W598_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type 11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363745.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24445"/>
    <dbReference type="EnsemblBacteria" id="AGZ43155">
        <property type="protein sequence ID" value="AGZ43155"/>
        <property type="gene ID" value="AFR_24445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4952"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1057310_0_0_11"/>
    <dbReference type="OrthoDB" id="1448234at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="44"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="28613" checksum="9C82811DD94D985A" modified="2014-01-22" version="1">MSAQARGWAGEVDGYEAYYVPRFAPWVAAATEAVAGAELPDGPVLVPCCGTFPELDALVTTMPGRELRGVDLSPGMVRRARERGAAHAQVSVVEGDASTLEPQSAAAVVSVFGLQQLPEPDAALRSWAEALRPGGRLAVVFWPAVTEDDGPFAIASELVRRHVPPHDWSWQERLVPALTAQGLTVDRDEEPSYPISHPDAAEFFDAYTRSGPMRSLADARGAEFVAGLRREFLARAPQGRWEHRPHARLIVARAPVSSPPSGAGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5W5</accession>
    <name>U5W5W5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="10">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">alaS</name>
        <name evidence="12" type="ORF">AFR_30710</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560737.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30710"/>
    <dbReference type="EnsemblBacteria" id="AGZ44402">
        <property type="protein sequence ID" value="AGZ44402"/>
        <property type="gene ID" value="AFR_30710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6218"/>
    <dbReference type="eggNOG" id="COG0013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_1_1_11"/>
    <dbReference type="OrthoDB" id="91428at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II_ALA" evidence="11">
        <location>
            <begin position="1"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="585"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="683"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="687"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ44402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="894" mass="96609" checksum="E99CFFB70475E746" modified="2014-01-22" version="1">MKTAEIKRRFLAHFEANGHTVVPSAPLPAIDDPNLLFINAGMVQFVPFFLGQRTPPYKRAVSVQKCIRTPDIDEVGKTSRHGTFFQMNGNFSFGDYFKAEAIRLAWELSTNPIEQGGFGLDPSRIWPTVYLDDDEAFEMWRAIGVPAERIVRRGKKDNWWHMGIAGPGGTCSELFYDRGPEYGKEGGPEVDEDRYMEYWNLVFMQYEITDVKSKENFTIVSDLPKKNIDTGMGLERVASILQGVDNLYEIDEVKPILARAAELSGKVYGAHSGHEASESHPDDVRLRVIADHVRTALMLIGDGVTPSNEGRGYVLRRIMRRAIRAMRLLGWQGATFPELLPVARDCMAPSYPELAEEFGRISTYAYAEEEAFLSTLRAGTTILDTAITDTKKSGGAKLSGAQAFQLHDTYGFPIDLTLEIASEQGLDVDQEGFRRLMADQRSRAKADAAARKTGHADLSAYRELLDNGGPVEFTGYAEVTRESRVRGLLGEGGTSLEAAGEGDYVELVLDTTPFYAEGGGQQADTGLINVGGGQVEVVDVQQPLPGLIVHKARVVRGEVRAGESGFAEIDVTRRRAISRSHTATHLIHQTMRAFLGESATQAGSLNAPGRLRFDFNTPGAVSPAVLHDVEQQVNEVLLRDLEVHAFITTQEEARRLGAMALFGEKYGDEVRVVEVGDYARELCGGTHVARSGQLGLVKILNESSIGSGVRRVEALVGIDAFGFLAKEHLLVSRLAELFRVPGDQVADRVEQTVTALRDAEKELEKMRAQLVLGGAGALAGEARDLRGVAYVGTEAPEGAATNDVRTLAQEIRGKIDNARPAVVAVAAKANGKASLIVAINAAAKSRGLSAADLVKGALSGRGGGNADLAQGGGVPADQVTVLLAAVEKAVGEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Q0</accession>
    <name>U5W6Q0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative signaling protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_26970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364572.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26970"/>
    <dbReference type="EnsemblBacteria" id="AGZ43655">
        <property type="protein sequence ID" value="AGZ43655"/>
        <property type="gene ID" value="AFR_26970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5465"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="353"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="496"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="765" mass="81581" checksum="5ECF6E1E7624A4A5" modified="2014-01-22" version="1">MTVLTTAPSTRGRRWWLLFGVAGVLATVLYTVNLGAAVSTAAFAVIGIGTVWALFAAPRRFRAEPRKAWPFMGAAGIFFLVGVVVRPAVESRPLLADAFTIPGYVLLGLFFVMLLRARGSIERHALLDGLMVCLAGGLASALLLAAPAAAIEGRPQLVSVLAGLYPLFDVVLLALGINLTFTARTWPVSLAALVAGMALMVVGDLAYAIIGVSGRTYASPLLDVPFLLAYTLLGVSALHPSVVELGRAARHPVQAWSWRRMLLLVPSVITPFVLLVTVGGRSVEYRMLIGACGIAIVALLLLRAVSAVQAQVAAQLHSEHQAMHDPLTGLPNRRMISAEIERLLAATPPEGTRRVWVYFLDLDGFKWVNDSWGHDTGDQLVIEVALRLRSAVPAGTMLARVGGDEFLIASVGDEQAALRLVEDIHRCFARPLPVRDTEVVISASIGIAHAPGDGTPAVTAEALQRDADTAMYRSKAEGPGRATIFDTSMHDRVRERIELEGALRTALAENQLSVVYQPIVRMETGRPVGAEALVRWNHPERGPIPPMTFVPVAEDAGLIGPLGDWVRNEALGQLSRWRADGTVGADFYLSINVSPRQLSQAELPMVVSGELMTHGVPASAVALEMTESVMADGGSVTAKVLFELRELGVQLLVDDFGTGFSALGYLRKFPVTGVKIDRSFVSGLGEGGEDEEIVRAVVAMGRALGLSIVAEGVETRVQRDALAAVGVTQGQGWLWGPGVPAGDFASHWSARQHASGAERLGARNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W716</accession>
    <name>U5W716_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561271.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33380"/>
    <dbReference type="EnsemblBacteria" id="AGZ44934">
        <property type="protein sequence ID" value="AGZ44934"/>
        <property type="gene ID" value="AFR_33380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6756"/>
    <dbReference type="eggNOG" id="COG2318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097062_2_0_11"/>
    <dbReference type="OrthoDB" id="2047691at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007061">
        <property type="entry name" value="DUF664"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04978">
        <property type="entry name" value="DUF664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="19167" checksum="56FB3D3B374091DD" modified="2014-01-22" version="1">MTDDRIGPPLLGGERETVRAFLDYHRATLEMKCAGLSDDELRQRSMPPSTLTLMGLLRHLAEVERTWFRRVINAEDIPLIWSDSGDFQAAYDPAGSTGAEAFTVWREEVEHSRRIEREAESLDVTGYQARWEEKVSLRLVMLHMIHEYARHNGHADFLREGVDGTVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7C6</accession>
    <name>U5W7C6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase S8 and S53 subtilisin kexin sedolisin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_34075</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34075"/>
    <dbReference type="EnsemblBacteria" id="AGZ45073">
        <property type="protein sequence ID" value="AGZ45073"/>
        <property type="gene ID" value="AFR_34075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6895"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_15_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666402" evidence="5">
        <location>
            <begin position="32"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="6">
        <location>
            <begin position="75"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="507" mass="51931" checksum="8A3FEA840847DA00" modified="2014-01-22" version="1">MADSTKRVVMSAVSAALAVLPLGVVATPASAAPPRPLARNWASWLGSTSTESATTLRQVRTVIGADTTATAALTGRGVGVALIDTGVAPVPGLPAGRIVNGPDLSMESQAPQLRYLDTFGHGTHMAGIIVGNDAATGTVGLAPDVKLTSIKVGTATGTVDVSQVIAAVDWVVAHRNDDTANPIKVINLSYGSGGTPNTWNDSLGFAVEKAWQAGIVVVAAAGNDGNGAGVLANPASDEWIITVGATATKGTATTYDDELTTFTNLPKAGKQINVLAPGTSILSLRDNGSSVDLANPSARVGDTLFRGSGTSQAAAVTSAAVALLLQAKPWATPDQIKEWLIRSATYLPYGTAAALGLKEINVNAALARSGSAVQAQSWNRSSGAGTLDNARGDSRVMFDNVPLSGQRSVWGPLATSGWAAKSAAGAAWAGGVWMGYRLAGDGWTGTSWASRTWSPAVWSNSAPWTGASTTWADPSWSGRSWSGRSWSAGAWSGRSWSSDDWSSASWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7K0</accession>
    <name>U5W7K0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561324.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33645"/>
    <dbReference type="EnsemblBacteria" id="AGZ44987">
        <property type="protein sequence ID" value="AGZ44987"/>
        <property type="gene ID" value="AFR_33645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6809"/>
    <dbReference type="HOGENOM" id="CLU_2091569_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="117" mass="12526" checksum="CE59FB63BA70AB08" modified="2014-01-22" version="1">MTLEIERAPLSRIDVAIKSPQELRDERRAAALRTEVILKITLVTLAWLVSVSLLLGWGLTGPEKQPVTVLLAALLAVVGPFVGAVIATRHGQFFLGGVYVVLTLAMVLPALSMVRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7L3</accession>
    <name>U5W7L3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560278.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28400"/>
    <dbReference type="EnsemblBacteria" id="AGZ43941">
        <property type="protein sequence ID" value="AGZ43941"/>
        <property type="gene ID" value="AFR_28400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5758"/>
    <dbReference type="eggNOG" id="ENOG50348D8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165400_0_0_11"/>
    <dbReference type="OrthoDB" id="1883804at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="96" mass="10892" checksum="00685662A8EF2C86" modified="2014-01-22" version="1">MIDLQVRDDSGDVVAHASGFEWTQELIDLEPAEFPMLAGLCAYLDTIFNQRQIPLLLAELDRLPATLIPDPARREIRRLAAVVEQGQHLYLWFCGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7T1</accession>
    <name>U5W7T1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33945</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561384.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33945"/>
    <dbReference type="EnsemblBacteria" id="AGZ45047">
        <property type="protein sequence ID" value="AGZ45047"/>
        <property type="gene ID" value="AFR_33945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6871"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="142"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="22652" checksum="EFBF7DDCC63156EF" modified="2014-01-22" version="1">MRIVLAEDSALFREGLARLLAEGGHEVVAAVADADALVAAVAEQRPDFAVVDVRMPPTMTVDGALAARRLREEHPGLPILLLSQHVETRHCVPLVATGGFGYLLKDRVLGVDDFLDAIRRVAAGGSALDPEVVAALLASTGRADPLAVLTPREREVLAHAAEGWSNGAIAARLSLAERTVETHMRSVLLKLRVPDSGDGHRRVLAVLTYLSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7W3</accession>
    <name>U5W7W3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35235"/>
    <dbReference type="EnsemblBacteria" id="AGZ45303">
        <property type="protein sequence ID" value="AGZ45303"/>
        <property type="gene ID" value="AFR_35235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7129"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050005_0_0_11"/>
    <dbReference type="OrthoDB" id="1702303at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="458" mass="49125" checksum="D6223D5957BD1202" modified="2014-01-22" version="1">MVLPRLTRPIFVHPAHNGGVGDVLIDLGEQHGRLPDVPAPRRRPAHRFRIALAVLTAVLLITITGAAPRPAPAEPITVPARIGDNMFVDRTRVYVVSAGPALHDSAVNNKIVSTYALPDGQLLSRTTVAVTGAIFSVMSVGETILVSYQVDTVGAEATVALTAGSDQALWRVPARLLSVSGPAGLVLLRENSPQFGDLHWYGVDLLTGRPRWTFEQPVQGYTTYAGSDSGFPPHLVTATTGGHIEVRDTVSGRLVTETDVEAPPGWSRRGITVWPVGDLLLVGGLGGVTAYALSDLSRRWHSSVELSGHWVQECVTAICVFGYQGGVEALDPVTGRVRWTADRWTNAEPVGRYLLVNGREGLEASYPLAVVEPETGRVHGDFGMWRSTGEVRADGTVVGLRQRIGEDLVFYALLDPATVSLRVLGSATHVSGDCRSTPEVLVCRRIDASLAIWPLTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W829</accession>
    <name>U5W829_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-ACP reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_35585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561709.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45373">
        <property type="protein sequence ID" value="AGZ45373"/>
        <property type="gene ID" value="AFR_35585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7201"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_0_11"/>
    <dbReference type="OrthoDB" id="968232at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="237" mass="24546" checksum="9899A534513C995B" modified="2014-01-22" version="1">MTNIALVTGANKGIGREIAAGLVELGTTVVIGARDEQRGKEAAAELGVESVVLDVTDAGSVRAAAGRLTDRFGRLDVLVNNAGISGGWEQAPSTVDPALVRAVFDVNFFGVLTVTNAMLPLLRRSPAARIVNVSSKGGSLTAQSDPDDPLHRSSPVSAAYVPSKAALNSLTVQYARELSSEGILVNLACPGFVATDLNGFRGTRTTEQGAAIVLRLATLGPDGTTGGFYDDNGRIPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8X1</accession>
    <name>U5W8X1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Urease accessory protein UreG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ureG</name>
        <name evidence="7" type="ORF">AFR_30925</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by UreG.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SIMIBI class G3E GTPase family. UreG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560780.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30925"/>
    <dbReference type="EnsemblBacteria" id="AGZ44445">
        <property type="protein sequence ID" value="AGZ44445"/>
        <property type="gene ID" value="AFR_30925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6260"/>
    <dbReference type="eggNOG" id="COG0378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072144_1_0_11"/>
    <dbReference type="OrthoDB" id="1134430at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01389">
        <property type="entry name" value="UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004400">
        <property type="entry name" value="UreG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31715">
        <property type="entry name" value="PTHR31715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00101">
        <property type="entry name" value="ureG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 4" id="KW-0996">Nickel insertion</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cobW" evidence="6">
        <location>
            <begin position="37"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="235" mass="24577" checksum="2346AC640F78C738" modified="2014-01-22" version="1">MHPDLHDHGPDEVPHTHPEPGVDPHAPLVNGPRALRIGIGGPVGSGKTALVAALCRALGEELRLAVVTNDIYTTEDADFLLRNGVLPADRIRAVETGCCPHTAIRDDISANLDAVEDLEATLGPLDLVLVESGGDNLTATFSKGLIDQQIFVVDVAGGDKVPRKGGPGVTTADLLVINKTDLAPLVGADLSVMDRDATARRGDLPTVFLSLVQDRAATPVADWIRALVAARIPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W900</accession>
    <name>U5W900_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561935.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36715"/>
    <dbReference type="EnsemblBacteria" id="AGZ45599">
        <property type="protein sequence ID" value="AGZ45599"/>
        <property type="gene ID" value="AFR_36715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7427"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_0_1_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TetR_C_1" evidence="1">
        <location>
            <begin position="102"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="262" mass="29065" checksum="93AFFF16BADB87E9" modified="2014-01-22" version="1">MTEFSGTDPDRILPLLWRHRSPAKGRTGRPPRLTVDAVVVAGLAIADAEGLEAASMARVAARLEVATMTLYTYVPSRAYLVELMVDEVLAGRRLPGPGDARPSGWREQIQLYADRTITMYREHPWLTEVSRVRPPLGPGMLRESEYVLSTLGGVPVERRNTAAVTITMFVTAAARQEGENAQLRRTDGQSNDAWWSERGQLWEDWFDVEQHPAMTELWNAGGFDRGPDQQAVDAFAYGLRLLLDGIEAQSSDTTGLRSSPMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9I0</accession>
    <name>U5W9I0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_37115</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37115"/>
    <dbReference type="EnsemblBacteria" id="AGZ45677">
        <property type="protein sequence ID" value="AGZ45677"/>
        <property type="gene ID" value="AFR_37115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7509"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072073_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041673">
        <property type="entry name" value="TetR_C_23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17931">
        <property type="entry name" value="TetR_C_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="43"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="256" mass="28418" checksum="D0B3CC13C8F7F44F" modified="2014-01-22" version="1">MTTAAADSPADEPGASPAAPEAKTPAAVEGDPGVEKPRRARGEQTRQLILETALRLFRERGYTETTMRAIAKEAGVAVGNAYYYFDSKEHLIQGFYDRNQAAHRVAAEPVLANEKDFAARLRGVLHAGIDVNEPYHSFAATFFKSAAEPSSPLSPFSRESSPAREAAISIFRDVVDGSSAKVDPELRKELPELLWLSWMGVVLFWVYDHSPEQRRTRRLIDGIVPLVDRLVALSRLRVLRPALRQILALIDSIRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9K2</accession>
    <name>U5W9K2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome P450</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31630"/>
    <dbReference type="EnsemblBacteria" id="AGZ44586">
        <property type="protein sequence ID" value="AGZ44586"/>
        <property type="gene ID" value="AFR_31630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6402"/>
    <dbReference type="eggNOG" id="COG2124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_638788_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002397">
        <property type="entry name" value="Cyt_P450_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00359">
        <property type="entry name" value="BP450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="SSF48264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="370" mass="38527" checksum="E864F6180ECAECCA" modified="2014-01-22" version="1">MNARSRDRRVYLAGHPVLFALLAATRSRPTWRLGRTLLVHDREAFTAALTRIPLDRTAEGTTGGAATRLTSGDLLFDQHGDTHRRTRRSTADSLGAAGVARLRPTWTEILDRGLKPLHDGDTVDLVPLTAELAGTTAAALIGRTLDGPSLAAAACAAAATAARAHVPGLPRFGAARRAREATERLSALVAPDGGPSSGLATMLAVAAITTTVAALPRAAAWTADANLWGYAHSPALVDELLRVTAPTPLLPRVAAAPGLLPASPGKPRAQGRSGGSGGCPVRAGDRMLLIARHAVEAHRLDPDPARPVPAQIAQLVFGVGPHACPGARLARAQLADLLQALAPLRPVVVKARADRHSALPSWRSLIVRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Q2</accession>
    <name>U5W9Q2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31905"/>
    <dbReference type="EnsemblBacteria" id="AGZ44641">
        <property type="protein sequence ID" value="AGZ44641"/>
        <property type="gene ID" value="AFR_31905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6458"/>
    <dbReference type="HOGENOM" id="CLU_208507_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="51" mass="5307" checksum="AD1967C04DAB9B1E" modified="2014-01-22" version="1">MAGLAGVAATGAVLARSERKRRAYTPDEIRGRLHARVAEAADEAPATTGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Y7</accession>
    <name>U5W9Y7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="1">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>9-ribosyl-trans-zeatin 5'-phosphate + H2O = D-ribose 5-phosphate + trans-zeatin</text>
            <dbReference type="Rhea" id="RHEA:48564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16522"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:87947"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N(6)-(dimethylallyl)adenosine 5'-phosphate = D-ribose 5-phosphate + N(6)-dimethylallyladenine</text>
            <dbReference type="Rhea" id="RHEA:48560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="ChEBI" id="CHEBI:57526"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38500"/>
    <dbReference type="EnsemblBacteria" id="AGZ45954">
        <property type="protein sequence ID" value="AGZ45954"/>
        <property type="gene ID" value="AFR_38500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7789"/>
    <dbReference type="eggNOG" id="COG1611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058336_0_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102682">
        <property type="term" value="F:N6-(Delta2-isopentenyl)-adenosine 5'-monophosphate phosphoribohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00730">
        <property type="entry name" value="TIGR00730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="24048" checksum="F0B31A8E061A14E1" modified="2014-01-22" version="1">MLDSHHRGEWKTKDAWRALRILSEFVEGFDTLADLPPAVSVFGSARSTPDSPECELAAQLGAALAEAGYAVITGGGPGVMEAANRGATEAGGMSVGLGIELPFEQGLNDWVDVGIDFRYFFVRKTMFVKYAQAFVVLPGGFGTLDELFEAITLVQTRKVTRFPVILMGTEYWGGLLDWIKGRLLADGKVGPGDMELMMVTDDVEEAVQIIVDADAALAENGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA74</accession>
    <name>U5WA74_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562262.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38365"/>
    <dbReference type="EnsemblBacteria" id="AGZ45927">
        <property type="protein sequence ID" value="AGZ45927"/>
        <property type="gene ID" value="AFR_38365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7761"/>
    <dbReference type="eggNOG" id="ENOG502Z890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063460_0_0_11"/>
    <dbReference type="OrthoDB" id="811477at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665853" description="DUF1996 domain-containing protein" evidence="1">
        <location>
            <begin position="37"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1996" evidence="2">
        <location>
            <begin position="81"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="33793" checksum="8437CCD9BC742DF2" modified="2014-01-22" version="1">MARRSRIFALGALVATAVTVAALPVLRSSSGGEAAAATAPTGYIRLDKPVQPIPVPASKLPFAPYREMHADCGVTKQAADDPLVFPGMPGASHLHSFVGNTAPEAFSTPKSLTEKGGSSCRDPKDTASYWFPTLLQNGVPVPNTTVTFYYKSGVTDYRTVRPFPAGFRVLVGDMKTPDAASFGGEWDCGNKGGTTREIPASCPKGNVLIIRYKAPSCWDGVNLDTPDHKSHLTYPVKGRCTPSHPVAVPMLQSKVAYKLEDGNTKGLKFSSGPSFSFHYDFMNGWDQDRLEELVEHCINGGRQCNGYGVDEHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAJ5</accession>
    <name>U5WAJ5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uma2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39795"/>
    <dbReference type="EnsemblBacteria" id="AGZ46213">
        <property type="protein sequence ID" value="AGZ46213"/>
        <property type="gene ID" value="AFR_39795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8051"/>
    <dbReference type="eggNOG" id="COG4636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076312_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06260">
        <property type="entry name" value="DUF820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012296">
        <property type="entry name" value="Nuclease_put_TT1808"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008538">
        <property type="entry name" value="Uma2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05685">
        <property type="entry name" value="Uma2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uma2" evidence="1">
        <location>
            <begin position="26"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="191" mass="21369" checksum="0E431A42F4DA6EF8" modified="2014-01-22" version="1">MTAQPAAHVMWTPDPIRQRLADHTVEDVLHLPDDAPRVELRDGVMIVVPSPTIGHQNIGNLLWFWLRQHAPDTFESVTAVGVAMGLRDSLEPDVLLIRQPVTLTNHFVLPSQVVVAVEVVSPGTRRRDGLEKPAEYAAARIPHYWRIEQDPIHVFAYDLVGNQYEPVADSDTELVLSAPFDIRLPISEITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAU2</accession>
    <name>U5WAU2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative flagellar assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fliH</name>
        <name evidence="3" type="ORF">AFR_40445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40445"/>
    <dbReference type="EnsemblBacteria" id="AGZ46343">
        <property type="protein sequence ID" value="AGZ46343"/>
        <property type="gene ID" value="AFR_40445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8186"/>
    <dbReference type="eggNOG" id="COG1317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103308_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018035">
        <property type="entry name" value="Flagellar_FliH/T3SS_HrpE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02108">
        <property type="entry name" value="FliH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FliH" evidence="2">
        <location>
            <begin position="85"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="23631" checksum="6F5BD70E9661DA99" modified="2014-01-22" version="1">MSSSPDDAPILRGTVAESATAARFGVDLRRGVPEDSAPVERAKQQARTAGYAEGWAQGQRAAALEAEAAAERARAVEQAHDQRRAAALAQAINALGRAVTGLETQMMPTLHELQEAVLAHAFELAEAIVGRSLDDPEGRAVSALRRAMNAAPDQGEIVVSLHPDDFRNLVGTATDADYNYEGRPVHLRPEPALRPGDAVAETGTTTVDASIATAVARAKEALRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB02</accession>
    <name>U5WB02_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative membrane lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_39590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BMP lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39590"/>
    <dbReference type="EnsemblBacteria" id="AGZ46172">
        <property type="protein sequence ID" value="AGZ46172"/>
        <property type="gene ID" value="AFR_39590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8009"/>
    <dbReference type="eggNOG" id="COG1744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038813_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003760">
        <property type="entry name" value="PnrA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02608">
        <property type="entry name" value="Bmp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Bmp" evidence="3">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="32888" checksum="A928064955DF96CB" modified="2014-01-22" version="1">MVTDTGGIDDRSFNASAWAGLQAAKEAQSSVDPKYVASTAEADYEPNLRQFVTQKCNFILSVGGLMGGATEKVAKENTSQQFGIVDSSIAGATNVFPMQFATEQAAFLAGYLAAGYSKSGKVATYGGLKIPPVTVFMDGFADGVAYFNKQKSKNVAVLGWDKAKQNGTFAENFGDQNKGKSITNTFVAQGADVVMPVAGGTGLGTADVAKASGGKVSVIWVDQDGCKSAEQYCDVFLTTVVKNITDAVEKAVADGASGKTLGSTPGYLGTLENEGVSLAPYNQFDSKVDAGLKSEVDKLKADIIAGTVKVESANAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB11</accession>
    <name>U5WB11_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561453.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34290"/>
    <dbReference type="EnsemblBacteria" id="AGZ45116">
        <property type="protein sequence ID" value="AGZ45116"/>
        <property type="gene ID" value="AFR_34290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6938"/>
    <dbReference type="eggNOG" id="ENOG5032YNM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102838_0_0_11"/>
    <dbReference type="OrthoDB" id="1967255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="23824" checksum="8B873D7EC9D545EA" modified="2014-01-22" version="1">MTDLRNHLEGIAGPAVPPTMTEIDADLKRGRHALRRRRAVQVAGGSTFAVAALVAAVAFAGSGAQTPGDSPQAGAPAVAEGVTHLVAYTGKQPKGFTIDKAPDGWFVQADDESSLLLAPSRIKGKPGVDPSQDPLYDDRSFVGKIAVMLQSKDEQGPRDGKAVKVGDKDGVLVKSLDSTDGRTLWVKQPKGAYLLIQFWDGIGLSQDEMVVFGAGVHVNKDAKQGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBG2</accession>
    <name>U5WBG2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">4-hydroxyphenylpyruvate dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_41150</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562816.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41150"/>
    <dbReference type="EnsemblBacteria" id="AGZ46484">
        <property type="protein sequence ID" value="AGZ46484"/>
        <property type="gene ID" value="AFR_41150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8329"/>
    <dbReference type="eggNOG" id="COG3185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034004_1_1_11"/>
    <dbReference type="OrthoDB" id="402639at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009072">
        <property type="term" value="P:aromatic amino acid family metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="PTHR11959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009283">
        <property type="entry name" value="HPP_dOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VOC" evidence="6">
        <location>
            <begin position="19"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="6">
        <location>
            <begin position="185"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR009283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="384" mass="42382" checksum="3C0EE24307EF86FD" modified="2014-01-22" version="1">MTQVMETATETVDVFPVKGVDYLQFLVGNAKQAAHYYSTAFGMTCVAYRGPENGYRDHAEYVLVSGSARFLITGAVHAGAPGADHVTRHSDGIYDIALHVPDVDKAFAHTVSAGARPVTEPHDLTDEHGTVRIAAIATYGDTIHTLIDRSRYDGPFLPGFVARSPIVDRQPAIDAGLQPKRYFQAVDHVVGNVELGKMDEWVAFYGRVMGFTNMAEFIGDDIATDYSALMSKVVADGTRKVKFPLNEPAVSQRKSQIDEYLEFYGGPGAQHIAVATNDILSSVDAMRAAGLEFLDTPDSYYDDPSLRARIGQVRVPIEELKKRSILVDRDEDGYLLQIFTKPVQDRPTVFFELIERHGSLGFGKGNFKALFEAIEREQELRGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBN1</accession>
    <name>U5WBN1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Diaminopimelate epimerase</fullName>
            <shortName evidence="8">DAP epimerase</shortName>
            <ecNumber evidence="4 8">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">dapF</name>
        <name evidence="10" type="ORF">AFR_35550</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="4 8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35550"/>
    <dbReference type="EnsemblBacteria" id="AGZ45366">
        <property type="protein sequence ID" value="AGZ45366"/>
        <property type="gene ID" value="AFR_35550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7195"/>
    <dbReference type="eggNOG" id="COG0253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053306_4_0_11"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="85"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="214"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="224"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="283" mass="29096" checksum="AEEF589F7FF024D7" modified="2014-01-22" version="1">MDRVLFTKGHGTGNDFVILGDPDGALDLTPELVAALCDRHRGIGADGVLRVVRSAKHPDGAGFAADAEWFMDYWNADGSIAEMCGNGVRVFTRYLLANGLATPGPSGLPVATRAGVVRAVIDDQIIAVHMRTPDVYAASTATVGPLTFPGVAVDCGNPHLVCGLHDSLPLSSLDLHHAPGFDPALFPAGVNVEFTTQGEPVEGADLHVNMRVYERGSAETLSCGSGALAVGAVALRDAGLTSGVVAVDLPGGRLTVTADNSAWWLAGPAVLVATGEVDLAALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBS6</accession>
    <name>U5WBS6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35995</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35995"/>
    <dbReference type="EnsemblBacteria" id="AGZ45455">
        <property type="protein sequence ID" value="AGZ45455"/>
        <property type="gene ID" value="AFR_35995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7286"/>
    <dbReference type="eggNOG" id="ENOG502ZGVP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1657108_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="14987" checksum="F45CFC7DFB3CBC0F" modified="2014-01-22" version="1">MKRRQLIGAAAGTALSATTLAGCGLFDDDPEPAPQPDALQPVLDEAVRLAAAYDRVALAQPGLAGRLTPLADDHRAHVAELSRVIGAAVPSGAPASSAPSGDAADSVASLRKAEQAAQKTAAALCRTAPAARAGLVGSIAAARAAHAEALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBU0</accession>
    <name>U5WBU0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_35850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35850"/>
    <dbReference type="EnsemblBacteria" id="AGZ45426">
        <property type="protein sequence ID" value="AGZ45426"/>
        <property type="gene ID" value="AFR_35850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7256"/>
    <dbReference type="eggNOG" id="ENOG5033408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098258_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021215">
        <property type="entry name" value="DUF2752"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10825">
        <property type="entry name" value="DUF2752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="18953" checksum="5FEE8A81035C19B9" modified="2014-01-22" version="1">MAPMSSTVTGPPPVPPQYPGYQEPQQDRVTRFIQRKWRDSPVWVAPAAMLVCMAGAVGYTYATSPTEANAGAAPTCLLKYATGFICPGCGGTRAAWYLLHGDIPAAARHHALFVFVVPFLLYMYVAWAGRRLFGWKIPQLNLSPGVIGVFIAAWGIFSVLRNLPWAPFTSFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDF1</accession>
    <name>U5WDF1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamate--cysteine ligase EgtA</fullName>
            <ecNumber evidence="2">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Gamma-glutamylcysteine synthase</fullName>
            <shortName evidence="2">GCS</shortName>
            <shortName evidence="2">Gamma-ECS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">egtA</name>
        <name evidence="4" type="ORF">AFR_38610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the synthesis of gamma-glutamylcysteine (gamma-GC). This compound is used as substrate for the biosynthesis of the low-molecular thiol compound ergothioneine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2 3">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; ergothioneine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the glutamate--cysteine ligase type 2 family. EgtA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38610"/>
    <dbReference type="EnsemblBacteria" id="AGZ45976">
        <property type="protein sequence ID" value="AGZ45976"/>
        <property type="gene ID" value="AFR_38610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7812"/>
    <dbReference type="eggNOG" id="COG3572">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037109_0_0_11"/>
    <dbReference type="UniPathway" id="UPA01014"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052699">
        <property type="term" value="P:ergothioneine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02034">
        <property type="entry name" value="EgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017809">
        <property type="entry name" value="EgtA_Actinobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035434">
        <property type="entry name" value="GCL_bact_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011556">
        <property type="entry name" value="Glut_cys_lig_pln_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34378">
        <property type="entry name" value="PTHR34378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34378:SF1">
        <property type="entry name" value="PTHR34378:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017901">
        <property type="entry name" value="GCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03444">
        <property type="entry name" value="EgtA_Cys_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR017901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="398" mass="42378" checksum="8D6D8D46890554E9" modified="2014-01-22" version="1">MHHVAQAADHISGICFKTGPPSRIGVELEWTVHRDGAPAAYVEAAELREALGRHAPPALGSTHPGDPLPGGGAVTVEPGGQVEISSAPAHSLTELYTSLSTDQSRLSALLARSGLVLGDSGLDPHRPPRRILNTPRYAAMQRRFDAQGRHGHTMMCSTAGLQVCLDAGPADRLPARWNALHEVGPALLAAFATSGRHAGRETGWASARMATWYGIEPRRTRPVTTSGDLATGWARYALAAPLLCVRNGADRWDAPPGVTFADWIGGALPRPPVIDDLDYHLSTLFPPVRPRGYLEVRYLDAQPGEEWIAPVAVLAALLSDDTITDAARALAAPTAGQWVSAARHGLADPVIRAGATDLLDLACRHLDSTGLPSGVRDVVTDIVDRRLVRAGLRKDLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDH8</accession>
    <name>U5WDH8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562340.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38760"/>
    <dbReference type="EnsemblBacteria" id="AGZ46006">
        <property type="protein sequence ID" value="AGZ46006"/>
        <property type="gene ID" value="AFR_38760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7843"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="31391" checksum="AD63324CA015EED1" modified="2014-01-22" version="1">MSDIDLRHLRYLIAVADEGGFTRAAERLGMTQPALSRAVRGLEDEVGVPLLIRLHRGVELTTAGKVLVEAARGITTAMETALRRSRQAAEESTRLRISARGCEVAAAEELKRGWAEPVEVLVTDRRSQSDVLREGGAELALIRGPFDDRGLDSDVLSTEPRIVLLHASHRLADSAVIARADLSPDPVVVWSGSGRQERDYWLGIDSGHRDVIVGPEVDDVLQMLARVRLGEAIAFAPLAMLRLLTLPDDVRTVPVDGLTDTEIRIVWVQEETSPAIARFVRYAVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE54</accession>
    <name>U5WE54_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Beta-ketoacyl synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_39760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562539.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39760"/>
    <dbReference type="EnsemblBacteria" id="AGZ46206">
        <property type="protein sequence ID" value="AGZ46206"/>
        <property type="gene ID" value="AFR_39760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8044"/>
    <dbReference type="eggNOG" id="COG2091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_31_5_11"/>
    <dbReference type="OrthoDB" id="1123553at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="SSF55048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="5">
        <location>
            <begin position="4"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="PKS_AT" evidence="6">
        <location>
            <begin position="555"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="887"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1076"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="895"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1001"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1083"/>
            <end position="1100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1669" mass="176077" checksum="6C6DFDDBE1467230" modified="2014-01-22" version="1">MEQIAIVGMAALFPGAGNLDEYWRNLVAGQDNISDVPEDRFDALFYDPDNAHRPDRLYTRRGGFLDDATFEPLKFGIMPNSVGDIEPDQLIALEVASAAIADAGGPEKMPDADRIGVILGRGGILSPAAARYAQRVRMSSQVIGILRELFPDLDEDRLDLVRARFDERLGKHQPEGTIGLVPNLAASRVANRLNLRGPAYTIDAACASSLLAVDQGMTELSNGRLDAVLAGGVHHVHDISFWSVFSQLQALSRKGTIRPFDADADGVLIGEGTGMVVLKRLADAERDGDRVYAVIRGSGTSSDGRSASMFNPASSGQTLAIRRAWEMAGLDPTAPDALGLLEAHGTATPTGDAAELTTVAEVFGPHVTGDRPVIGSVKSMIGHAMPAAGVAGLIKATLAVYHGTLLPTLNVTNPRSEMAATRFQPIPAARPWESEGPRRAGVNAFGFGGINAHVIIEQHAAPVGAVQVREPERVLRLAAGSVPELAALLDRPDVRDLPQDTEAGPVRLGLFDPSDQRLGIARKMVARGEAWRGGRDIWFTPRPLLGAGGGKLAFVFPGVEAEFAPRTADVAAHFGLPHREWTAADLGQHGTGLVEVGRQLDIALRRMNIKPDAVAGHSIGEWTAAAVTGQMSTQGVDAFLGIFDADSVEVSGYAFAAISAGVESIEPVLVDYPGVVLSHDNAPHQCVVNGPRDQVQRLVEDLRARTILCQLLPFRSAFHTPVFAEGLQAIGAALGHWEVHPTEVPVWSGTTSAPFPTDPDEVNALFIRHMIEPVRFRQTVDAMYASGIRVFLQVGAGQLASLIDDNLRDREHLAMPVNVSRRSGLEQLHRVATALWVDGGAPDLAAITPAAAPARVNTVAGPAARKGPAIKLDLGGPLVKLGEGADRLLAPSGTPTAQRPMSPAPTSALPTQRPAIPAQRPASTTQRPPVPAQRTATAAQGPAVAAQGVAAPAQRAGVAAQRPAPPAQRPANPGQRPAVPAQGAAAPVQRPAGSTPRTVVPAQRPPAPAPSHVAPRPEPQRPAVPAASAGASAEAHSAALSALGKLAGSSKAAAELAALLQETANDAVTVMTAATTTNRATPAAPPRVPPAAPPRGVPATPARGVPAAQTRTAPAAPSRTAPGVPGRVGPAAPGSGHASLPGSGSDGPGSRASAVPATGRLGEPGTSTRPVPEKVFKTTLDVSVETMPYLRDHCFFVQPDDWPQVEDRWPVVPATTVVQHMMDAAEQAVPGLKAVAVLEAKFHRWLIAAPAQQVEITVKTIAPNRVAVVFGGYAKSIVELAADYPEPAEAPWTHDPATERPPTTSAETMYAERLMFHGPQFQGVTAVHALGDMHVRGVVTAPVPPGALLDNALQLIGNWLITTQPFRTVALPVGLRHVKFYGPPPAAGTAFECVARVREITDGELIADTQLSVGGRVWAQIDGATDRRFDSHPTARACERFPEKFPMSQRRPEGWTLVFDAWTDLVTRGMAARGILGGEAAVEYERMPGARKKQWMLGRIAAKDAVRFRQWDAGHTDVYPIELTVRNEPSGRPYVELRPGRGLVESEVSIAHTAEIGVAIAGDKGSEVGIDVTEIVARDDSTIRYALTDAEIAALGPDRELAFARMWAAKEAVGKALGTGLDGAPRRFVTDIEAGTVTAGDRTFRVGTTVVENPPELPPRRYVVAWTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNC7</accession>
    <name>U5VNC7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-binding region ATPase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357416.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00920"/>
    <dbReference type="EnsemblBacteria" id="AGZ38473">
        <property type="protein sequence ID" value="AGZ38473"/>
        <property type="gene ID" value="AFR_00920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.183"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133205_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HATPase_c" evidence="1">
        <location>
            <begin position="14"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="13147" checksum="3E45DB873A753C92" modified="2014-01-22" version="1">MPEVLLEQSFDEDTLYGVRSAVAAHVAAVTDDRTVDTMVLIAHELASNAVRHGGGSGRLRLWVADGALLCEVTDAGPGLDEPAYAGQTLPPPELPGGRGLWIARQMSDLRISTSPAGSVITATLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNJ5</accession>
    <name>U5VNJ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_00350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357303.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00350"/>
    <dbReference type="EnsemblBacteria" id="AGZ38359">
        <property type="protein sequence ID" value="AGZ38359"/>
        <property type="gene ID" value="AFR_00350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.72"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="101"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="19576" checksum="A81EFF0254570B5A" modified="2014-01-22" version="1">MVRSRRFDGLDTLIAERGNALLGTAVLLTGNRAAGEDLLQAALERLMRSWGRVREDRERYLRRTMYHLAIDQWRHRRRRPEVFLGHEPPGGPDATDALDLRDALIMALAQLSPRQRAVLVLRYWEQLTEPETAAELGCSVGTVKSTASRGLARLRELTSTWDLHHATLNGTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPX5</accession>
    <name>U5VPX5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03595"/>
    <dbReference type="EnsemblBacteria" id="AGZ39008">
        <property type="protein sequence ID" value="AGZ39008"/>
        <property type="gene ID" value="AFR_03595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.723"/>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092418_0_0_11"/>
    <dbReference type="OrthoDB" id="1865374at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="50"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="20960" checksum="EB4C9BC3AEAC9653" modified="2014-01-22" version="1">MENLWLQKIAENPGHSRWYIDRFRKLAKDGADLAGEARLIDTMVPRGSRVLDAGCGAGRVGGFLAAAGHDVTGVDLDPELIAAAEEDYPGPRWLTGDLAELELPERFDVIVCAGNVMTFVAPGTRVEILRRLRLHLAEGARVVIGFGADRGYAFDDFFVDVAAAGLAPDVLLSTWDLRPFGAGSDFLVALLRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ93</accession>
    <name>U5VQ93_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357922.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03450"/>
    <dbReference type="EnsemblBacteria" id="AGZ38979">
        <property type="protein sequence ID" value="AGZ38979"/>
        <property type="gene ID" value="AFR_03450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.693"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087580_2_1_11"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="179" mass="19933" checksum="D98A3DBE5C865D79" modified="2014-01-22" version="1">MEEPARLNDARVMRALAHPIRLRIIALLSEIGPASVSALAGHTGESTASVSYHLSQLARFGLVEEAVEARTGRERPWRAVHRGLSWDWNDGDHGLRAAYLKERLRVVDEFVAAEDALTPEWKTAAFFLDDVGHLTTAELAEVSDRIKALLAGYRRPDPADRPADAQRVVFFAYGLPHER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQN2</accession>
    <name>U5VQN2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_04225</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04225"/>
    <dbReference type="EnsemblBacteria" id="AGZ39134">
        <property type="protein sequence ID" value="AGZ39134"/>
        <property type="gene ID" value="AFR_04225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.853"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="6"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="96"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="18709" checksum="5BFBAC2646AC5887" modified="2014-01-22" version="1">MSFDELYAAHYGDLTVQLYAYFGDRQEAQDVVQEAFCRALARWRTVSRYDDPVAWIRRVAWNLAVSKWRRTRTALGFLRRQRVTEAQVAGPSPERVALVAALGTLPPAQRRAMVLRYLADLTVAEIAEREGVAEGTVKSWLHRARASLAAQLAPTETTHQEVRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQU4</accession>
    <name>U5VQU4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358146.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04440"/>
    <dbReference type="EnsemblBacteria" id="AGZ39177">
        <property type="protein sequence ID" value="AGZ39177"/>
        <property type="gene ID" value="AFR_04440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.898"/>
    <dbReference type="eggNOG" id="ENOG5033MFI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1208689_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="23770" checksum="2B56CC82AB25C134" modified="2014-01-22" version="1">MTALVRMRLAGFLRSGRALAPIITALVVLGIIHGGGRSPAAPAYGYSAVMLFPILAWQTKLVLDAEPDVQRRLARLAVGARREAAAGLLAAAVLGLACCAVAMLIPWWPFHAISTPEPGTTSLFAGIAVGAFAHLLTIPAAVALGALASRAVTRTVRNGITVLVSGAVLAIVLGLSGSIAPWLVPPVMATARALAGDTLPSGGTLWLLTGWALAWCAAVLTGYARLRRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQY8</accession>
    <name>U5VQY8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="4">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">truA</name>
        <name evidence="9" type="ORF">AFR_04665</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358225.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04665"/>
    <dbReference type="EnsemblBacteria" id="AGZ39222">
        <property type="protein sequence ID" value="AGZ39222"/>
        <property type="gene ID" value="AFR_04665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.942"/>
    <dbReference type="eggNOG" id="COG0101">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_0_2_11"/>
    <dbReference type="OrthoDB" id="1075262at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PseudoU_synth_1" evidence="8">
        <location>
            <begin position="152"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="29833" checksum="928F9F29424CD428" modified="2014-01-22" version="1">MDETTRLRLDVSYDGRDFSGWAVQPSRRTVAGVLLEALERLTGPDVALGLTVAGRTDAGVHATGQVCHIDLPTATWSALESSLVRRLAALMPPDARVRAVTPVPSTFDARFSATFRRYEYRIADTTFGPEPLRRHDTLWWVRPLDLDRLQQAADGLVGLHDFAAFCKRKEHATTVRAITRLDWRREADGVAVATVQADAFCQAMVRSLVGAMLTVGDGRRAPEWPAKLLSLRERSSEVTVAPAHGLTLIAVGYAAEADYAERAEATRRLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRF2</accession>
    <name>U5VRF2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Iron permease ftr1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_05750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358970.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05750"/>
    <dbReference type="EnsemblBacteria" id="AGZ39439">
        <property type="protein sequence ID" value="AGZ39439"/>
        <property type="gene ID" value="AFR_05750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1167"/>
    <dbReference type="eggNOG" id="COG0672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077905_0_0_11"/>
    <dbReference type="OrthoDB" id="2018349at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="PTHR31632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="290" mass="31283" checksum="23DCD075C240F52A" modified="2014-01-22" version="1">MLATYLIGLREGLEATLVVSILIAFLVKSDRKDRLVQVWAGVGLAVALSVFFGWLLSYTSTTLLQKYEHRELFEAVTSILAVVFVTWMIFWMRKAARSIAGELRGKLQDALAVGSISVALMAFLAVVREGLETSLIFYSAVQGASDNSRPLYALLAGIASAVAIGYLMYATAVRINLSVFFTWTGALLILVAAGILKYGVHDFQEANVLPGLNTLAYDISGVLDPSAWYTALLTGMFNITPTASVLESIAWAAYGIPVLVLFLRPSRKKPAGEKPVATTSEPTPASAPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRP3</accession>
    <name>U5VRP3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_00555</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Set transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00555"/>
    <dbReference type="EnsemblBacteria" id="AGZ38400">
        <property type="protein sequence ID" value="AGZ38400"/>
        <property type="gene ID" value="AFR_00555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.113"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055598_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004750">
        <property type="entry name" value="Sugar_efflux"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23535:SF2">
        <property type="entry name" value="PTHR23535:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="9">
        <location>
            <begin position="19"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ38400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="404" mass="42338" checksum="6340F618CA363EF1" modified="2014-01-22" version="1">MAAMAQETSTRTATRLGRALLPLGLLFLAVGISVALFNPFLALFLSTEVDAGPVKTTVFLIVAPLAGVLGSSLVGRVSDRRPIRRRLLIIGAVAGVVGTGVTAVVRDYWVLLALAITAFALAGSLFPQSFAYARQVLLRDDPARAPMGISALRTVFSLAWVAGPSLAAFLLDSGSFVWVYGTAALTYAFAALIAIFWLDEVPAPAPPAADEPAAVSGDAPRWTLWLTAAAFTLLQTPLTLAVQALPLFISTELGGDVGDAGLLLGLCAALEIPLMLGLGLLSTRLPLRLLLFIGAACGIGYYGIAAVSTGLWMLVAGQVLNALFIAAVSGLGISYMQDMLPHHPGRATTLFTNSFPLGAMLAGPLFGLAQHFDYRLAYWLCTALCAAGLLLLIVVRNDHATRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRQ8</accession>
    <name>U5VRQ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06065"/>
    <dbReference type="EnsemblBacteria" id="AGZ39502">
        <property type="protein sequence ID" value="AGZ39502"/>
        <property type="gene ID" value="AFR_06065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1232"/>
    <dbReference type="HOGENOM" id="CLU_2821410_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026334">
        <property type="entry name" value="FxSxx-COOH"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04268">
        <property type="entry name" value="FxSxx-COOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="68" mass="6858" checksum="7204730745CB95B9" modified="2014-01-22" version="1">MDGGTSTTGATPEWRSAMVDVSGLSLAELGADTAPDPADDSALAQSLRRLADDLARPGEPIAGFNSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS26</accession>
    <name>U5VS26_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359175.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06775"/>
    <dbReference type="EnsemblBacteria" id="AGZ39644">
        <property type="protein sequence ID" value="AGZ39644"/>
        <property type="gene ID" value="AFR_06775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1384"/>
    <dbReference type="eggNOG" id="ENOG50334PR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2115736_0_0_11"/>
    <dbReference type="OrthoDB" id="1911904at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="114" mass="12286" checksum="0411DA59D4812E80" modified="2014-01-22" version="1">MSDNTAPEDLGVPQSFLVLDDGTAVYDRAGDKVGTVEHVLADDQEDIFHGVVVKTSDGHRYASAAQIDGIFEHGVIVAVPAAELKEPAENPAPDDDSSFTEGLRKAWNWLIQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSD3</accession>
    <name>U5VSD3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359439.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08095"/>
    <dbReference type="EnsemblBacteria" id="AGZ39908">
        <property type="protein sequence ID" value="AGZ39908"/>
        <property type="gene ID" value="AFR_08095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1652"/>
    <dbReference type="eggNOG" id="ENOG5031VYU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168932_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="86" mass="8977" checksum="A7AAFC426C5AE9A5" modified="2014-01-22" version="1">MTTPPSENLLRRLARVNPTTAFLIALALLLAGLFLPGIIGAALLFVLGAGLAALTFTTWPVQSPGVRAIRVTLLVLLFAAVVAKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSE8</accession>
    <name>U5VSE8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">DNA primase</fullName>
            <ecNumber evidence="12">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dnaG</name>
        <name evidence="17" type="ORF">AFR_08170</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 13 14">Binds 1 zinc ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer. Interacts with DnaB.</text>
    </comment>
    <comment type="domain">
        <text evidence="12">Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="12"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359454.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08170"/>
    <dbReference type="EnsemblBacteria" id="AGZ39923">
        <property type="protein sequence ID" value="AGZ39923"/>
        <property type="gene ID" value="AFR_08170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1667"/>
    <dbReference type="eggNOG" id="COG0358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013501_3_1_11"/>
    <dbReference type="OrthoDB" id="1071997at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03364">
        <property type="entry name" value="TOPRIM_DnaG_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00974">
        <property type="entry name" value="DNA_primase_DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013264">
        <property type="entry name" value="DNA_primase_core_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037068">
        <property type="entry name" value="DNA_primase_core_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019475">
        <property type="entry name" value="DNA_primase_DnaB-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006295">
        <property type="entry name" value="DNA_primase_DnaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013173">
        <property type="entry name" value="DNA_primase_DnaG_DnaB-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030846">
        <property type="entry name" value="DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034151">
        <property type="entry name" value="TOPRIM_DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002694">
        <property type="entry name" value="Znf_CHC2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10410">
        <property type="entry name" value="DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08278">
        <property type="entry name" value="DnaG_DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08275">
        <property type="entry name" value="Toprim_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01807">
        <property type="entry name" value="zf-CHC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002811">
        <property type="entry name" value="DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00766">
        <property type="entry name" value="DnaG_DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00400">
        <property type="entry name" value="ZnF_CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01391">
        <property type="entry name" value="dnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 12 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 12 13" id="KW-0639">Primosome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 12 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="16">
        <location>
            <begin position="265"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CHC2-type" evidence="12 14">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR002811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR002811-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ39923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="615" mass="66946" checksum="7DE1D3D2A486075E" modified="2014-01-22" version="1">MAGRVKDEDIALVRDRTSIADIINETVTLRSAGGGNLKGLCPFHDEKTPSFTVSPARNVYFCHGCGAGGDAIKFLMDAEHLTFIESVERLAGRAGIQLRYDESGFKNDGPRQQPGQKQRLIAAHAAAAEFYQEQLTTPGARKAREFLAQRGFGRDAAERYACGFAPDGWDLLTKHLRQRGFTAEELTAGGLAKPARSGSLIDRFRRRLLWPIRDLTGDVIGFGARKLFDDDDGPKYLNTPESPLYKKSHVLYGIDHAKREMAKQGRAVIVEGYTDVMACHEAGEPTAVATCGTSFGIDHIGVLRRLLMDSDSFTGEIIYTFDGDAAGQKAALRAFEEDQRFVGRTFIAVSPDNMDPCELRLARGDLAVRDMIASREPLVDFALRQTLARFDLDTVEGRVEAMRRAAPLVAKIKDREKRPEYARKLAGDLGMDLEPVQRAVASASSPESSGAPAPRRPVDSPQRLVEREALKLALQHPVLAGPMFDALGPETYGDEVFQGIRKAIAVAGGTTTATGGAGWIESVRDACDDLGGKALVGELAVVPLHVDGEADLRYVQVTLARLQVGAVTTRIKELKSKVQRINPVAHKDEYLALAGELFSLEQHARALRDQAAGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSQ8</accession>
    <name>U5VSQ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative anti-sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01885"/>
    <dbReference type="EnsemblBacteria" id="AGZ38666">
        <property type="protein sequence ID" value="AGZ38666"/>
        <property type="gene ID" value="AFR_01885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.381"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119460_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HATPase_c" evidence="1">
        <location>
            <begin position="8"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="15576" checksum="E3C37B185E9F8E8E" modified="2014-01-22" version="1">MATVRLSFSPAPVHVRTARLVGVAVARRAGVAEELLDEVRLAIGEACTRAVALHRQYGLPDLVMVEMSDSDAYTVRVIDRAPIEASVGLTKLPPDELADESLTDEALTTGVGFALLAGFVDDLQVRPVEEGAGTEVRMVWPVSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSV6</accession>
    <name>U5VSV6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative cobalt transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_08040</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08040"/>
    <dbReference type="EnsemblBacteria" id="AGZ39897">
        <property type="protein sequence ID" value="AGZ39897"/>
        <property type="gene ID" value="AFR_08040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1641"/>
    <dbReference type="eggNOG" id="COG0619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="27746" checksum="441938366F357EED" modified="2014-01-22" version="1">MTLVLEPVADATAALARRNPVAKLGAALIFSLPLVATLDPLTPAIALVIELAVLPFFGVRYGVLARRAWPLSLAAAGILVTMVLFAADRSGSLLFSLGPFAITTGVVLSALGLVLRVFAVALPGIIVFATTDPTDLADALVQNAKAPARFAIGALAAFRLIPLLGEEWQMLGLARRARGIDAGRNPLAKLRLFGSTAFALLVGALRRGGRLAVAMDARGFDSDTPRTYARRQVFGAADWLLLAGAALLSAGALTASVLTGLFRPIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSW8</accession>
    <name>U5VSW8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HATPase_c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02260"/>
    <dbReference type="EnsemblBacteria" id="AGZ38741">
        <property type="protein sequence ID" value="AGZ38741"/>
        <property type="gene ID" value="AFR_02260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.457"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133205_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HATPase_c" evidence="2">
        <location>
            <begin position="36"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="17626" checksum="119A3335F76EFA3C" modified="2014-01-22" version="1">MLRPAPGRESPDGTAVPANPDRQFTLLDRTFGRDEITVVRHEVTARLDRAGLGDRLSGFVLAINEVITNVVLHAGGHGRIVLWGTSGSLWCTVTDSGPGIPERYRHPPEVPEAFEVGGRGIWLAHQLCDEVTMATGPIGTTIGLRVIVSPRNNTSDLVNGASTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSZ3</accession>
    <name>U5VSZ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359604.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40073">
        <property type="protein sequence ID" value="AGZ40073"/>
        <property type="gene ID" value="AFR_08920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1816"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_15_0_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16764" checksum="FCDBB5460FC21609" modified="2014-01-22" version="1">MADDDAALGMIETNVAMLMRLGEATRRSSPRPHRALDRAAYVILRFLQEAGPQNVSAVATRLNLDGSTVTRQVGALHRDGLVERSADPEDGRGTVIAATDKGLSQVDKVSQARRELYGSVLKDWSPKERRELAVTLERLIHDMDAYLKGRGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT90</accession>
    <name>U5VT90_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative HTH-type transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_08715</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359563.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08715"/>
    <dbReference type="EnsemblBacteria" id="AGZ40032">
        <property type="protein sequence ID" value="AGZ40032"/>
        <property type="gene ID" value="AFR_08715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1773"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_3_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="187" mass="19859" checksum="E2B84FF75DCA779F" modified="2014-01-22" version="1">MDDKRRLILDQALALVDEHGLGAMSMRAVAERVGLTSMALYPYVGGKDAMLDGLVDLLHLELGSEYGEDPAGIDWRQRLRALGRAVRALSRAHPGAYPLLLNRPAAGASASWLTAALRGCLHDAGVPDGQVPRLTRMICAFLLGYTVGEVTGGLPADEAAGHDDDAAEFDADLDDLVRLVEVTVSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTA3</accession>
    <name>U5VTA3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase M48 Ste24p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_03380</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357908.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03380"/>
    <dbReference type="EnsemblBacteria" id="AGZ38965">
        <property type="protein sequence ID" value="AGZ38965"/>
        <property type="gene ID" value="AFR_03380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.680"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052979_0_0_11"/>
    <dbReference type="OrthoDB" id="1918093at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M48" evidence="8">
        <location>
            <begin position="75"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="327"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ38965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="370" mass="40369" checksum="FCD5AC767A490366" modified="2014-01-22" version="1">MTIDDDNRPARQRVTLTDISSRAWEHPADRGALTALRELRGFDDVVKAFFGMWNERGFRLSYLAGSIRVDHRQYPRVYQRYAEAATTLDVAELPELFVTQSPMIHGQAIGMDKPFIVITTGAVEKLDDDELRALLGHEIGHVRSGHAVYKTIMMILTKWATSVSWLPIGAIALRAIIAAMYEWWRKAELSADRAGLLAGQDPSASIRLLMKLAGGGDLSQIDTAAFLEQAAEYQGGGDLRDSIHKISMTAWSSHPVPVARAAELRRWIDSGDYARIVGGDYPRRDSDGNASVTGDVKAAASHYRESFQNSQDPLVTLARRVTGGAADLGDWAGTQAGKARAWASTAADAAGRAARRESRPADNPGDTPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU76</accession>
    <name>U5VU76_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">DNA-directed RNA polymerase subunit beta</fullName>
            <shortName evidence="6">RNAP subunit beta</shortName>
            <ecNumber evidence="6 8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">RNA polymerase subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Transcriptase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 15" type="primary">rpoB</name>
        <name evidence="15" type="ORF">AFR_04460</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6 8">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="6 8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358154.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04460"/>
    <dbReference type="EnsemblBacteria" id="AGZ39181">
        <property type="protein sequence ID" value="AGZ39181"/>
        <property type="gene ID" value="AFR_04460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.901"/>
    <dbReference type="eggNOG" id="COG0085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_4_1_11"/>
    <dbReference type="OrthoDB" id="9601at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01321">
        <property type="entry name" value="RNApol_bact_RpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042107">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_ext_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019462">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_external_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010243">
        <property type="entry name" value="RNA_pol_bsu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10385">
        <property type="entry name" value="RNA_pol_Rpb2_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02013">
        <property type="entry name" value="rpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA_pol_Rpb2_1" evidence="12">
        <location>
            <begin position="35"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_2" evidence="11">
        <location>
            <begin position="161"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_3" evidence="13">
        <location>
            <begin position="406"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_45" evidence="14">
        <location>
            <begin position="484"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_6" evidence="9">
        <location>
            <begin position="611"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_7" evidence="10">
        <location>
            <begin position="1031"/>
            <end position="1105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ39181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1146" mass="127274" checksum="1642ED54173E4CDB" modified="2014-01-22" version="1">MAASRPAKTSRTSSAYAPRRISFGRITEQLEVPNLLALQTDSFDWLVGNEAWQARTTDDPHAHSGLAEILEEISPIEDFSGTMSLSFSAPRFDEVKASIEECKEKDLTYCAPLFVTAEFTNNTTGEIKSQTVFMGDFPMMTPKGTFVINGTERVVVSQLVRSPGVYFTKEPDKTSDRDLSSVKVIPSRGAWLEFDIDKRDTVGVRIDRKRRQAVTVLLKAIGWSAEQIRERFGWSELIMTTLEKDHIAGQDEALLDIYRKLRPGEPPTRENAQTLLDNLFFNPKRYDVAKVGRYKFNKKLDIDVPIVRGTLTEEDIVKTVDYLARLHAGEEGYEADDIDHFGNRRLRTVGELIQNQVRVGLSRMERVVRERMTTQDVEAITPQTLINIRPVVAAIKEFFGTSQLSQFMDQTNPLAGLTHRRRLSALGPGGLSRERAGFEVRDVHTSHYGRMCPIETPEGPNIGLIGALSTFARVNPFGFIETPYRKVIDGTVTDNVDYLTADEEDRYVKAQANAPLKSDGTYAEDRVLVRRKGGEIDYVPGEQVDYMDVSPRQMTSVATAMIPFLEHDDANRALMGANMQRQAVPLVKAEAPLVGTGMEYRAAVDAGDVVVAEVGGLVEDLCADYVTVHQDDGHRRTYLLHKFRRSNAGSCVNQKPVVFEGDRVEAGQVIADGPCTDEGEMALGRNLLVAFMCWEGHNYEDAIILSQRLVQQDVLTSIHIEEHEVDARDTKLGPEEITRDIPNVSEEMLADLDERGIIRIGAEVVPGDILVGKVTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGETGTVIGVRTFSREDGDELPPGVNELVRVYVAQKRKIQDGDKLAGRHGNKGVISKILPIEDMPFLEDGTPVDIVLNPLGVPSRMNIGQVLETHLGWIAKTGWEVEGDDADWKRQLRAIEAHESPKDSNVATPVFDGAQEEEIKGLLNSTLLNRDGKRLVNADGKAQLFDGRSGEPLPDPISVGYVYILKLNHLVDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMECWAMQAYGAAYALQELLTIKSDDVLGRVKVYEAIVKGENIPEPGIPESFKVLLKELQSLCLNVEVLSSDGVALEMRETDDEVFRAAEELGIDLSRRPNEGVSSVEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUD3</accession>
    <name>U5VUD3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_05230</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05230"/>
    <dbReference type="EnsemblBacteria" id="AGZ39335">
        <property type="protein sequence ID" value="AGZ39335"/>
        <property type="gene ID" value="AFR_05230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1058"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="33518" checksum="36656C33A9FAC02E" modified="2014-01-22" version="1">MTTVELKNVSRWYGNVVAVNDITMTLEPGVTGLLGPNGAGKTTVLHMMAGFLSPSRGEVTIDGAPTWRNPDIYRRLGLVAEREAVHGFLTAREFVQASARMQKLPDPKAAAEWALQMVEMTDAADRRIETYSKGMRQRTRVAAALVHNPQVLLLDEPFNGMDPRQRMHMMGLLHELGDRGHTILFSSHILEEVEQVSGTVQVIVAGRLAASGDYRRIRRLMTNRPHVFALQSSDDRRLAVALISEKSVSGVEIDNTGLTVRASDYGSFTRALPRIALGEGIRLRKLLPSDESLESVFSYLLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUF8</accession>
    <name>U5VUF8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360588.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11495"/>
    <dbReference type="EnsemblBacteria" id="AGZ40588">
        <property type="protein sequence ID" value="AGZ40588"/>
        <property type="gene ID" value="AFR_11495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2343"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006462_2_3_11"/>
    <dbReference type="OrthoDB" id="1573900at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022567">
        <property type="entry name" value="DUF3459"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11941">
        <property type="entry name" value="DUF3459"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="3">
        <location>
            <begin position="29"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="561" mass="61305" checksum="2668DED8CE19ED04" modified="2014-01-22" version="1">MTDQLTVPSAAPSPAAEPSPWWRDAVIYQIYPRSFADSDGDGIGDLPGIRSRLPYLRDLGVDAVWLSPFYASPQADAGYDVADYRTVDPMFGTLADAAALITDAHGLGLRVIVDLVPNHSSDQHEWFRRALAEGPGSPLRDRYHFRAGQGPDGSLPPNDWRSIFGGPAWTRTTNADGSPGQWYLHLFAPEQPDFNWDHPAVADEFRTILRFWLDLGVDGFRIDVAHGLVKAEGLPDVGATTEWHLLDVGVSPCFDRDGVHDIYRSWRRILDEYPGERIAVAEAWAPSLDRVANYVRPDELHQAFNFSYLGTAWEAGAQRQVIDDSLATLRTVGAPATWTLSNHDVVRHTNRLLQTDAGDVAGRRPTAVDPVAGLRRARAATLLLLALPGSAYLFQGEELGLPEVVDLPPEVRQDPAFHRASGQDGYRDGCRVPIPWEGDRAPYGFGPPDGPSWLPQPASWAALSVAAQQGDPGSTLELYRAALALRRADPALGAADNLSWRDAPDGLLVFDRAPHFRCTVNMTAAPVRLPLPGRALLTSGPVDLSGTEVEIPADTTIWWAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUK4</accession>
    <name>U5VUK4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360694.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11795"/>
    <dbReference type="EnsemblBacteria" id="AGZ40648">
        <property type="protein sequence ID" value="AGZ40648"/>
        <property type="gene ID" value="AFR_11795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2402"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079365_0_0_11"/>
    <dbReference type="OrthoDB" id="1389691at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038740">
        <property type="entry name" value="BioF2-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13480">
        <property type="entry name" value="Acetyltransf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="124"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="30037" checksum="9773198ABEAAA801" modified="2014-01-22" version="1">MTDLDALLAMHDEWMRIPPQIPLPGVGYEWDGRLLRITGQMRGMLRGPRDIGVEGDDLDRLIQRQVDYFGARGEAFEWKVRGHDLPADLPNRLLAAGFVPELQSAVLIGFSEQIAAEPVLPDGVQLRRVTAEADMRRIAEMQSAVWGGDLSWIGDYLWGLVVTTPDDIVVLVAEAAGEVVCAAFILCGGEGSYGALLGGSTLPAWQRRGIYRALVHARAQIAVERGLELLQVDASPHSAPILQKMGFHQVSTSALYAWAPPQAQGSSNSQSSNGGSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUM3</accession>
    <name>U5VUM3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05705"/>
    <dbReference type="EnsemblBacteria" id="AGZ39430">
        <property type="protein sequence ID" value="AGZ39430"/>
        <property type="gene ID" value="AFR_05705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1157"/>
    <dbReference type="eggNOG" id="COG3564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134343_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008497">
        <property type="entry name" value="DUF779"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05610">
        <property type="entry name" value="DUF779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009151">
        <property type="entry name" value="DUF779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12847" checksum="0AB60E991B369D5F" modified="2014-01-22" version="1">MTPAAADLMRSLRGQHGPLMFHQSGGCCDGSAPMCYLEGEFRTGASDVLLEELHIDGVDEPISFWMSTSQYQAWKHTHITVDVVPGRGSGFSLEAPEGVRFLIRSRVLTPEEVAALPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUW7</accession>
    <name>U5VUW7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome hibernation promoting factor</fullName>
            <shortName evidence="2">HPF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hpf</name>
        <name evidence="4" type="ORF">AFR_05785</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase; 100S ribosomes are translationally inactive and sometimes present during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with 100S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HPF/YfiA ribosome-associated protein family. Long HPF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358977.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05785"/>
    <dbReference type="EnsemblBacteria" id="AGZ39446">
        <property type="protein sequence ID" value="AGZ39446"/>
        <property type="gene ID" value="AFR_05785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1174"/>
    <dbReference type="eggNOG" id="COG1544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071472_0_0_11"/>
    <dbReference type="OrthoDB" id="1766263at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00552">
        <property type="entry name" value="RaiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00839">
        <property type="entry name" value="HPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034694">
        <property type="entry name" value="HPF_long/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003489">
        <property type="entry name" value="RHF/RaiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032528">
        <property type="entry name" value="Ribosom_S30AE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038416">
        <property type="entry name" value="Ribosom_S30AE_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16321">
        <property type="entry name" value="Ribosom_S30AE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02482">
        <property type="entry name" value="Ribosomal_S30AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="SSF69754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00741">
        <property type="entry name" value="yfiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1 2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Ribosom_S30AE_C" evidence="3">
        <location>
            <begin position="146"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="23598" checksum="134BBF76BEE1386F" modified="2014-01-22" version="1">MDIVVKGRNVEVPDHYREHVADKLAKIERYDQKLIRVDVELFHERNPRQSDNCQRVEITVVTRGPVIRAEACAKDFYAALDLAFNKLDGRLRRAADRRRVHRGRHAPVSVAAATAGLPTDLPSLTPQDEESSVATDLLEHDNDQPWRIVREKEHPADPMTVDDALFQMELVGHDFYLFHDKESGRPSVVYRRRGYDYGILSLETTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVT7</accession>
    <name>U5VVT7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_13515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361048.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13515"/>
    <dbReference type="EnsemblBacteria" id="AGZ40989">
        <property type="protein sequence ID" value="AGZ40989"/>
        <property type="gene ID" value="AFR_13515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2744"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="26507" checksum="686001B3042B6FE9" modified="2014-01-22" version="1">MTNSTATTATGLLAGKVIFITGASRGIGAAAARLFTQEGAAVVLAARSTDALDRLVTEIRAEGGVADAITVDLADSSSIRAAIARVEELHGRLDGAFNNGATIQQPGPLDTTSDADIDEQFAVNFRAHWTAMNAEATLMRQGGGGAIVNTSSIGSRRANPFLPAYGAMKRALNSITESAAVTWAPAGIRVNGITPGGTTTEMMQAWDEATPGVIAANIAATPMGRMAEPREVAEVAAWLLSDRASMVTGAIVPVDGGAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVZ1</accession>
    <name>U5VVZ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14255"/>
    <dbReference type="EnsemblBacteria" id="AGZ41133">
        <property type="protein sequence ID" value="AGZ41133"/>
        <property type="gene ID" value="AFR_14255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2892"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060110_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="31821" checksum="A50707076EA21050" modified="2014-01-22" version="1">MIITHQRRDEALAAVERLVALPEQPPVVLVDNGSTDGTADAVRARFPQVDVLALAWNAGAVGRNLGVGRLTTPYVAFCDDDTWWESGSLTTAADVLDAHPQLAVLNARIVVEPSGREDSIVAELRDSPVPGPDWLPGPALGSFLAGASVVRRDAFRAVGGFSSRLWLGGEEELLATDLLSAGWEICYLEDLVVHHAASPIRDTVRRRRDGLRNTLWFTWLRRPLAPALRRTLFLAVTAPHDRISALAWWDAVRGLPWVLTRRRTRPRSVEARLAPLDAAQRASTARRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWF3</accession>
    <name>U5VWF3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative cold-shock DNA-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_14535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361248.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14535"/>
    <dbReference type="EnsemblBacteria" id="AGZ41189">
        <property type="protein sequence ID" value="AGZ41189"/>
        <property type="gene ID" value="AFR_14535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2952"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_0_3_11"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="67" mass="7311" checksum="DD630AE787A6FE5F" modified="2014-01-22" version="1">MTTGTVKWFNADKGFGFITPDDGGADVFAHFSAIATSGYRSLDENQKVEFDITQGQKGLQAENIRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWG0</accession>
    <name>U5VWG0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">zf-CGNR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359571.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08755"/>
    <dbReference type="EnsemblBacteria" id="AGZ40040">
        <property type="protein sequence ID" value="AGZ40040"/>
        <property type="gene ID" value="AFR_08755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1781"/>
    <dbReference type="eggNOG" id="COG5516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087298_3_1_11"/>
    <dbReference type="OrthoDB" id="1478254at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010852">
        <property type="entry name" value="ABATE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023286">
        <property type="entry name" value="ABATE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021005">
        <property type="entry name" value="Znf_CGNR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35525">
        <property type="entry name" value="PTHR35525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07336">
        <property type="entry name" value="ABATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11706">
        <property type="entry name" value="zf-CGNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160904">
        <property type="entry name" value="SSF160904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-CGNR" evidence="1">
        <location>
            <begin position="152"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="21848" checksum="77EFD49C9B2975AC" modified="2014-01-22" version="1">MRVRHPDGQVFSFDAGALCLELSSISGGEGFRARFELLHSPGDFVRWAFAGRLDLAGWGLRPEDVVATEEHLALVKRLREAIWAGAWATATGSAIARVDADVLNEVAGGAVPVPEIGPELRRTWQRPVRADQLATLFARDAVTTLTPPTATRVRMCAADNCALIYLDTSRPGNRRWCSMERCGNRAKVRSHRSREDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWL2</accession>
    <name>U5VWL2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ubiquinone/menaquinone methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09005"/>
    <dbReference type="EnsemblBacteria" id="AGZ40090">
        <property type="protein sequence ID" value="AGZ40090"/>
        <property type="gene ID" value="AFR_09005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1832"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057148_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="34"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="29344" checksum="2E621D2B7C6D3995" modified="2014-01-22" version="1">MAAVYTHGHHESVLRSHRWRTAENSAAHLLPRLKSGDTLLDIGCGPGTITGDLATLVTPGRVTALEITAPALDLARAEITRRGLGNVDFAVGDVHALDFPDDTFDVVHAHQVLQHVGDPVAALREMHRVTRPGGVIAVRDSDYAAFTWFPQMPELDEWLDLYQRVARGNGGEPDAGRRLLSWAHAAGLTDVTATSSTWCFATPEDRQWWGGMWADRILESDMARTAREKHGATEADLQRISEAWRTWAAEDDGWMSLLHGELICQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWL7</accession>
    <name>U5VWL7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361462.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15605"/>
    <dbReference type="EnsemblBacteria" id="AGZ41403">
        <property type="protein sequence ID" value="AGZ41403"/>
        <property type="gene ID" value="AFR_15605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3169"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_2_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="30623" checksum="8784B183C8E5001C" modified="2014-01-22" version="1">MTADLSDRTFVVTGASSGLGLATTRALAARGAHVVLAVRDEEKARRVAPGHEIEHVDLADLDSVRDFAARLHDRHPRIDVLINNAGVMAPPRTLSPQGHELQFATNHLGHFALTGLLLDLLLAGRDPRVVTVTSVNHRQAKPPFGERAYSPMGDYNTSKLANAIFGVELHRRLTAAGLPLSSVLAHPGYTVTNLQFSATTPMWRFLLGRIGNPLLAQPVESGARPLLFAATDPAAQPGQLIAPDGPGELRGRPAPGKLAPAATDPENGRRLWELSEKTTGVRFDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWR6</accession>
    <name>U5VWR6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SMI1_KNR4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361507.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15830"/>
    <dbReference type="EnsemblBacteria" id="AGZ41448">
        <property type="protein sequence ID" value="AGZ41448"/>
        <property type="gene ID" value="AFR_15830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3214"/>
    <dbReference type="eggNOG" id="COG4282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1479077_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09346">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SSF160631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMI1_KNR4" evidence="1">
        <location>
            <begin position="27"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="19631" checksum="42EB83D89231D147" modified="2014-01-22" version="1">MPEPTGQEILELLRLVPHAPGDTPSPGATEEQVAAVQARYPMALPPAYLEWLRLCNGSMSGPGGIYGVGVGKEWIDALVVLDGHPGWAELGWVPVAGDGNGNTYVLDASRSHIDRDAVFFVDAMGDGGLTYLAGSSLMVSFRGLLRKEAREEDRWPFDANYVSATDPHILLVRDAGLLPWFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX56</accession>
    <name>U5VX56_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15475"/>
    <dbReference type="EnsemblBacteria" id="AGZ41377">
        <property type="protein sequence ID" value="AGZ41377"/>
        <property type="gene ID" value="AFR_15475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3142"/>
    <dbReference type="eggNOG" id="ENOG503260E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082059_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="207" mass="22385" checksum="5807BC5802081476" modified="2014-01-22" version="1">MVALLSGFVLPVGLYYLLRTLGVGPLLALLAGGVPTLVRIVLTGVRERAIDRISVFTLSLLVAGALASLLTGSPRWLLAKGGVFTGIIGVWLLWSLRGRTIAFEGILGFQRTAAAVEAWEANWRDSPEFRHVMRAVTVIWGAGFLVEAVVRIVLAYTLPVDVVPVVTSVQFLVLIVLMLWIGPRYGRGYMSRRGLTTGPDGIRTVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX89</accession>
    <name>U5VX89_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Antibiotic efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361643.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16430"/>
    <dbReference type="EnsemblBacteria" id="AGZ41568">
        <property type="protein sequence ID" value="AGZ41568"/>
        <property type="gene ID" value="AFR_16430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3332"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_13_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="416" mass="43405" checksum="1CEAFB788E65EA59" modified="2014-01-22" version="1">MAATRGSGLFHCYWASVFVSYLGDGIRLTAFPLLAVSLTTDPGLVAVVSAAATVPWLLFGLHAGVLVDRVPRLPLMVVLQLVRAAAGTAAAVGVVTGRLTVAELAVIAFVMGTCEVGYDMASQAVLPELVPADRLQWANSRLVSTEAVVLEFAGPALGGVLFAVAASLPVAVDTVTLLASAALLGSMAVKSMARLKGKPRETVVPEPVSQQLKDGVRWFLRSPLIRSITVLTTCINLGSGGLEAVLVLFARNHLGLGPAGYGLLIAVGAIGSFAGGLLAERVVTARARRTAILWTAPVNGTCFVLIGLFPDRVVTFAMFIAFGITISVFRVHASSLRQSKVPTEMLGRVLGVHRVVCWGALPVGALGSGAIASAYGIRWAIIASGATVVLSWLVLIRFLARPAVDEYTIAESVSRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX99</accession>
    <name>U5VX99_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360151.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10330"/>
    <dbReference type="EnsemblBacteria" id="AGZ40355">
        <property type="protein sequence ID" value="AGZ40355"/>
        <property type="gene ID" value="AFR_10330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2103"/>
    <dbReference type="eggNOG" id="COG3393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_993264_0_0_11"/>
    <dbReference type="OrthoDB" id="1563557at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013653">
        <property type="entry name" value="FR47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08445">
        <property type="entry name" value="FR47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="161"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="30402" checksum="A1DAC5A6BC37B6D9" modified="2014-01-22" version="1">MALEDTDVYTRLERFYDAVPRDAAVVEDIGGFVLFVNDGWGWPYYARPRLGGAETPSAADITAVRRRQRELGVPEAFEWVHETTPDLLAVARSAGLDVLLAPLMVLESAALVPDLPLPGATIRFLDPASATFAQDISISRAVGRLGFGAPANATAVQTGSLAIEVAGPAQRDAVPPLSEEAIAQQVRRHASGRFVSAVVESPADGILACGAVQRVESVAEIAGVATLPSARRRGYASQLTASLARRTLLDGSDLVLLSAGDDDVARLYSKVGFRRIGTACIAEPAAAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXD8</accession>
    <name>U5VXD8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AFR_15875</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361516.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15875"/>
    <dbReference type="EnsemblBacteria" id="AGZ41457">
        <property type="protein sequence ID" value="AGZ41457"/>
        <property type="gene ID" value="AFR_15875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3223"/>
    <dbReference type="eggNOG" id="COG3290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020211_11_1_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033463">
        <property type="entry name" value="sCache_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016120">
        <property type="entry name" value="Sig_transdc_His_kin_SpoOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039506">
        <property type="entry name" value="SPOB_a"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17203">
        <property type="entry name" value="sCache_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14689">
        <property type="entry name" value="SPOB_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55890">
        <property type="entry name" value="SSF55890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="328"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ41457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="519" mass="54948" checksum="BA5E96C31C945965" modified="2014-01-22" version="1">MRQRLSLAGQLLILQLAIVLLVVGAVAAVSITEADAAFRRVEGERLRSVGENTAINRTVRLGLGNPLGREALETVAESARAVSGASYVMVTDAAGTIVTGPDAGRTAPMGDSTGLTGRSWVGVSTEGGKSLTAHVPVLDERDGRFLGLIIVGRTYPTLADQLTAALANLLTYLVLGIVLGVGGSLLLARRVKRQTLGLEPREITGLVEHREAMLHGIKEGVIGTDSAHRVTLANDEAIRLLGLPADAVGRSLHDQDLEPALLDVLTGRSEGIDQVVLSDDRVVVLNRQPVVMRDRTVGSVTTLRDRTELTALRRELDVSRHTTDTLRAQAHEFVNRLHTIAGLVELGEHDEVVRYITRTSELHESLDREVTEVVGDTALAALLIAKASLAGEQGVTLSIGDGTDLPPVSDRLSADLVTVVGNLVDNALDATGPGGAITVTVRESDGEVAVTVVDTGPGVDPELADRVFQQGYSTKDEKHGHHGLGLAMIRLICVHRGGTVQVSGSTFTARLPYELEAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXJ1</accession>
    <name>U5VXJ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PKD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_16410</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361639.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16410"/>
    <dbReference type="EnsemblBacteria" id="AGZ41564">
        <property type="protein sequence ID" value="AGZ41564"/>
        <property type="gene ID" value="AFR_16410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3328"/>
    <dbReference type="eggNOG" id="COG4771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307927_0_0_11"/>
    <dbReference type="OrthoDB" id="913951at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665452" evidence="3">
        <location>
            <begin position="24"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="30"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="122"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="22058" checksum="8E3157EC1BD76ADD" modified="2014-01-22" version="1">MRIAATLALIAGAVLVTPAAAGAAGVDHTPPTRPGPLVFDLTSGLTISWAPSTDDRGPVTYEVYESNSLRTTTTGTSYVYSTGGTLPPRIFIFAVRAVDPAGNPSPYVYRTVGQIWSGTEIPAAPTDLRVEAQSRRLLKLSWTGPAETSPFVQPPVAGYEVFLDGVPIGETGATSLVVARPRPGAHTYGVRTINAVDQRSPVTAERSGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXL4</accession>
    <name>U5VXL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mannose-6-phosphate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17175"/>
    <dbReference type="EnsemblBacteria" id="AGZ41713">
        <property type="protein sequence ID" value="AGZ41713"/>
        <property type="gene ID" value="AFR_17175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3484"/>
    <dbReference type="eggNOG" id="COG1482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020529_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="34496" checksum="D0F783F0F7A5BB36" modified="2014-01-22" version="1">MVSAPDAPVVLPANTLPSFYAGAGRIAAFRGDPAVPATDPEDWVASATARTGMAPRGRTRLPDGTDLGERFAADPQGWFGPGHVARHGDRAALLVKLLDAGQRLPLHVHPDRPFAQAHLGSPYGKTEAWLVLRADPGASVHLGFARDVTAAELAGWVSTQDVAALLDACNRVPVRAGDVLLCPAGVPHAIGAGILVVELQEMTDFSIMLEWDGFPIAPEDVFLGLDAELALSAVRRTAVPESELAGHVLRAEPGITSLLPAAADEFFHAQQIVGEATLDARFAVLIVDRGAGTLTGPGVTEITAGQTLVVSYAAGPLTLAGDVSVLRCFPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXW2</accession>
    <name>U5VXW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360452.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11110"/>
    <dbReference type="EnsemblBacteria" id="AGZ40511">
        <property type="protein sequence ID" value="AGZ40511"/>
        <property type="gene ID" value="AFR_11110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2263"/>
    <dbReference type="eggNOG" id="COG2513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027389_2_3_11"/>
    <dbReference type="OrthoDB" id="1485205at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="24712" checksum="FB55A51938E9049A" modified="2014-01-22" version="1">MTDFRDLHVPGKPLVLVNAWDAASARIVEAAGAVAVATTSAGVAWSLGFPDGDALDRDLAVAAVARIAAVVSVPLSADIEKGYGDVAETVRLVQAAGAAGVNIEDGVTAEPDRIAAARAAAPDLFLNARIDTFLAGAGGLDETLERAAAYLAAGADGIFVPGVTDPDTVAALVEGIAAPVNVMAGPGAPSIAELAELGVARISLGSAVAQAAYGLVRRAALGVGDYSDLAGAEDYGVLNSLLTPGASGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXX0</accession>
    <name>U5VXX0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Arabinofuranosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_17825</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362159.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17825"/>
    <dbReference type="EnsemblBacteria" id="AGZ41843">
        <property type="protein sequence ID" value="AGZ41843"/>
        <property type="gene ID" value="AFR_17825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3615"/>
    <dbReference type="eggNOG" id="COG3940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081200_0_0_11"/>
    <dbReference type="OrthoDB" id="1832114at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046556">
        <property type="term" value="F:alpha-L-arabinofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046373">
        <property type="term" value="P:L-arabinose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007934">
        <property type="entry name" value="AbfB_ABD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036195">
        <property type="entry name" value="AbfB_ABD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05270">
        <property type="entry name" value="AbfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110221">
        <property type="entry name" value="SSF110221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="AbfB" evidence="3">
        <location>
            <begin position="125"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="255" mass="27358" checksum="CCC7072F21618599" modified="2014-01-22" version="1">MPVSPAGAHRLAPRLRPRTVVAGAATLSALFVIGLVLAPEEKEARRPPMFIALPTVPEATVPAADPSSVPASVPSSTAAPRPFPSRSTVRVTSDSISTVRPPAARRVASPRPSLPVVGSSIGLGSASVPGLRVRHRDYRAYLEEVGARSSAQDRADSRFTVRPGRARTGCLSFESQNFPGYFLRHRNFVLRLDRVDGSALFDADTTFCAVPVTGGGFVLRAHNYPDHYLTERDSLLSLIRTRPAEATAFVTRPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY31</accession>
    <name>U5VY31_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative LuxR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11605"/>
    <dbReference type="EnsemblBacteria" id="AGZ40610">
        <property type="protein sequence ID" value="AGZ40610"/>
        <property type="gene ID" value="AFR_11605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2365"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="95"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="17647" checksum="1FD014094940DA38" modified="2014-01-22" version="1">MPARAAELWPTVIVIDTEYMVSQVLPIAEELHTAIPSCSMLLLCDPAKRGMLPPRRWAGGLNFVLKDASDAVLADSIRRLAAGERVVSPMLQAASLNTDRGLSTRELEVLGLAAEGESVREIAGRLYLSGGTVRNYLSAVITKTGARNRLDAIRIARKGGWLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYI3</accession>
    <name>U5VYI3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17820</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362157.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17820"/>
    <dbReference type="EnsemblBacteria" id="AGZ41842">
        <property type="protein sequence ID" value="AGZ41842"/>
        <property type="gene ID" value="AFR_17820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3614"/>
    <dbReference type="eggNOG" id="COG3087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_859519_0_0_11"/>
    <dbReference type="OrthoDB" id="1523132at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="34150" checksum="B5E6250237B082AE" modified="2014-01-22" version="1">MDNTDSDEIYRRPAAPASPTFPSISDYWPDAPHRIGPPADHYSDEPTAASVDSLLRKSRTFHPVAATSAPPPPRRRGRRVYGFVLAAVLLAGAGLFVAKVARDQEREPGPVRAAPTPVTTTPNSLSAPARGRTEARFELVSDTATIDLSTAALGADLYRISTPERGSVVPRATIGDTGVRLFLDDNGERGDAAVKVLLNTDVRWSLRISGGVRQGVFDLGGAKVGTVDFAGGAARIDLTLPAPDGTLPIRMSGGVNRFEVRTGRDVPVRVRTRKGAGQVKLDGRTDDGVARGAAFLSPGWSRSTDRIDLDAVAGVGTLRVGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYK1</accession>
    <name>U5VYK1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glucose-methanol-choline oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18455</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18455"/>
    <dbReference type="EnsemblBacteria" id="AGZ41969">
        <property type="protein sequence ID" value="AGZ41969"/>
        <property type="gene ID" value="AFR_18455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3745"/>
    <dbReference type="eggNOG" id="COG2303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002865_7_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="PTHR11552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMC_OxRdtase_N" evidence="4">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="518" mass="56429" checksum="0A925C1FB38AFA00" modified="2014-01-22" version="1">MPALHTDEFDVVVVGGGTAGAVVASRLSADPGVRVCLVEGGPSDVGDDRVLQLRNWLNLLETEYDYDYPTVEQPRGNSHIRHSRARVLGGCSSHNTMISFVPPPGDFADWVAAGATGWSWDEMQTYWQRLAVQIQPVAAKDRNPLTEAFVASCHTALGVPVHEDFNTKPFADGTGFFPVAYYPETGVRSSSSVAYLHPYLDRPNLTVLTETWASRLDVDGDRVTGVRVTRDGHEEVVRGAEYVLCAGAIDTPRLLLLSGIGPATDLRDLGIEVALDVPGVGEHLLDHPESLILWESTRPIPPQSAMDSDAGLFVRRDTGDERPDLMFHLYQLAFTVNTKRLGYDVPEHGFGMTPNIPRPRSVGRLWLTSADPSVKPALDFGYFTDPDGYDEQTIVDGLRIAREVAATEPFKSWIAREIAPGPHLQTDEELSAYGRAAAHTVYHPAGTCRMGAISDDLAVVDPELRLRGCTNVRIADASVFPTMPSVNPVVAVLMIGERAADLVAEGLARTQEHPPPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYM1</accession>
    <name>U5VYM1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MbtH-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362362.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18555"/>
    <dbReference type="EnsemblBacteria" id="AGZ41989">
        <property type="protein sequence ID" value="AGZ41989"/>
        <property type="gene ID" value="AFR_18555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3766"/>
    <dbReference type="eggNOG" id="COG3251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181321_1_0_11"/>
    <dbReference type="OrthoDB" id="2067093at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005153">
        <property type="entry name" value="MbtH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038020">
        <property type="entry name" value="MbtH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037407">
        <property type="entry name" value="MLP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38444">
        <property type="entry name" value="PTHR38444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03621">
        <property type="entry name" value="MbtH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00923">
        <property type="entry name" value="MbtH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160582">
        <property type="entry name" value="SSF160582"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MbtH" evidence="1">
        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="70" mass="7794" checksum="68BB09CB23EAC571" modified="2014-01-22" version="1">MSTNPFEDPEGTYLVLVNDEGQHSLWPAFVEVPAGWETVLPEGPRDAALEYIDTTWTDMRPKSLIAAMDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYP5</accession>
    <name>U5VYP5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative nitrite reductase small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18690</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362387.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18690"/>
    <dbReference type="EnsemblBacteria" id="AGZ42014">
        <property type="protein sequence ID" value="AGZ42014"/>
        <property type="gene ID" value="AFR_18690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3794"/>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_1_11"/>
    <dbReference type="OrthoDB" id="1681886at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="4">
        <location>
            <begin position="2"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="11215" checksum="2159C97833357941" modified="2014-01-22" version="1">MTRLGPVDDIPLGEGRTYAVDGEMVAVFRLRDGSLRALQAVCPHRGGPLADGQIDLKVVVCPLHLNAFDLTTGCSLSGQPDLTVYAVTVDATDHLVISSLASAEVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYZ7</accession>
    <name>U5VYZ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative cobalamin synthesis CobW-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362447.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19000"/>
    <dbReference type="EnsemblBacteria" id="AGZ42074">
        <property type="protein sequence ID" value="AGZ42074"/>
        <property type="gene ID" value="AFR_19000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3858"/>
    <dbReference type="eggNOG" id="COG0523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_2_0_11"/>
    <dbReference type="OrthoDB" id="1267701at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="Cbl_biosynth_CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="1">
        <location>
            <begin position="252"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="399" mass="43589" checksum="A60717316B5C7F3E" modified="2014-01-22" version="1">MSTRPITTVAPSPVDRRPRVTVLAGFSPHATEAVARSLLVTDPRLLLVRHDLTRVRDGHVRRIVRTATTLLEDETVHLVHGCVACTLSEDVLPTLVRLSRTHPASDVILALPGVVEPESLARCAAGAPVRFDSYVTVVEADGFLDELSTTDDLRDRGLHAADEDRRSVASVVARQVEYADTIVVWGRPDHRTETMLHHLAPWATQLRVGDTTTIDCTGLAARVRGTGRHDPRKPGLLGRALEGYPIGVHDPGDSMLFRARRPFHPQRLFDALPELTGEALRGRGQLWLATQPDVAIGWESAGGGISLTSLGYWLDALDAERWDEASDLRRLAADLDWDPYHGDRRTVLAFIGLDGDADDLTARLTDCLITDDELAGGSEARAGISDPFAGFFPVDPEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ73</accession>
    <name>U5VZ73_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase S1 and S6 chymotrypsin/Hap</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13720"/>
    <dbReference type="EnsemblBacteria" id="AGZ41030">
        <property type="protein sequence ID" value="AGZ41030"/>
        <property type="gene ID" value="AFR_13720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2786"/>
    <dbReference type="eggNOG" id="COG5640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1076176_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665510" evidence="1">
        <location>
            <begin position="22"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="30"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="26653" checksum="0AB0AD46D6B1C354" modified="2014-01-22" version="1">MLALTAAVASTFFVASSPATAAFSSGPVRITGGTLEDDSAAESGWMATIVTPAKPCSATLIAPSWVLTAGECFSGDVPGAAPGSVAGPDDVVRAAGQTRKVTWASHRGYLTLARLDRPIQSAYYPPLAQTDPVAGARTYMYGAGTGCVMCRQVGRYRSAPLTVIQNDYWNLYYGSTLLSASPVGTFTLGDFGGAQMQDGVLAGVLVGSGETQYNTAPTNVISYKVAPARSWILQTKAANPPPGTIVTVPTCPVFVCGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ95</accession>
    <name>U5VZ95_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoside-diphosphate-sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13600"/>
    <dbReference type="EnsemblBacteria" id="AGZ41006">
        <property type="protein sequence ID" value="AGZ41006"/>
        <property type="gene ID" value="AFR_13600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2761"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025711_4_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="5"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="203" mass="21542" checksum="77AB5B9C4EAAB516" modified="2014-01-22" version="1">MVVLGASGATGRHVVSTALSRGHQAVALVRRPGTFTPQEKLLEVTWPDVKDTATLTAALPGADIVISTLGGAGNGPTTVCTDGIRSAVRAMGATGVNRLIALSAHGVLETHDKSLYSRAVWANVAERMRDKETMEPLITASGLNWTIVRPPKLSDHDAIGKYTAGTDLPIRLWSSIGRADLAAFLIDEAETPRYAHACPRVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W046</accession>
    <name>U5W046_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362881.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21185"/>
    <dbReference type="EnsemblBacteria" id="AGZ42509">
        <property type="protein sequence ID" value="AGZ42509"/>
        <property type="gene ID" value="AFR_21185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4295"/>
    <dbReference type="eggNOG" id="COG3541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084778_1_0_11"/>
    <dbReference type="OrthoDB" id="974296at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018775">
        <property type="entry name" value="RlaP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34817">
        <property type="entry name" value="PTHR34817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10127">
        <property type="entry name" value="RlaP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="24691" checksum="29223CA7F09D1A75" modified="2014-01-22" version="1">MITLVTEHTILAVVVGSRAYGLAGPGSDHDRRGVYAAPTRMFWHLDKPPTHLDGPAEEQFSWEIERFCTLALQANPTVLEVLWSPLISSVTADGRELLAVRSAFLSQRVASSYGSYARDQLKRVAARRSKTGETNHKQAMHMIRLLLAGAHVLRTGEVLVDVSPLRSRLLAVRQGEVPWESVVAWADTLFEDLTAAAATTTLPPLPDRAAIDNLLHSVRERGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0D9</accession>
    <name>U5W0D9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme F390 synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15570"/>
    <dbReference type="EnsemblBacteria" id="AGZ41396">
        <property type="protein sequence ID" value="AGZ41396"/>
        <property type="gene ID" value="AFR_15570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3162"/>
    <dbReference type="eggNOG" id="COG1541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036174_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="509" mass="57066" checksum="2D75C23D34E73A54" modified="2014-01-22" version="1">MRQWTLRIASSALSGLYTLYRIHPAAWRLMARNYRPQLGRFARLNAWMTCQFAALDVPAYRDFLGRNGWAFRWFELTSYPPTGKRSYVDAYPENERCWHGELEIAGSVVDESSGSSGRPYNWVRGRRELRSVHKNLAGYATMVFPSKRRFVVNAYSMGAWATGTNTGIAMARIAMVKNTGPDLRKIVDTLRHFGPGFEYIVTAYPPFLKHLRDELDAEGFPWADYRISGLVGGEGMTEALRDYLEERFHKVRSGYGASDLTIGMAGETDFSVWVRRRLRTDAALRTALLGPDEHRLPMVFQYNPLETYLETTADSEILCTINSMAVLTPKVRYNIGDEGRLATFPEVLAAITDPGARAEAEAAFRVDRMRLPLLFLFGRKDSTISYMGANIYPQDVEYGLYTGNTLAHLLQGFCLELREHADLESRPIVHLQLRDDAVLDAAQRSGLAETCRAGIVAHLTAVSRDFAESLAEDPSAADLRVAVHDYATGPFTDANNKIKSAYLVKGGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0E5</accession>
    <name>U5W0E5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361519.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15890"/>
    <dbReference type="EnsemblBacteria" id="AGZ41460">
        <property type="protein sequence ID" value="AGZ41460"/>
        <property type="gene ID" value="AFR_15890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3226"/>
    <dbReference type="eggNOG" id="COG0110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_9_1_11"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="202" mass="20477" checksum="01446D58A83B5EBA" modified="2014-01-22" version="1">MADVSPDGATVAPTADVDPRATIGAGTRVWHLAQIREDATLGSNCTIGRGAYVGPGVVVGDNVKLQNHALVYEPARLGDGVFIGPAAVLTNDEYPRAVTPDGRLKSGDDWTAVGVTIGEGAAIGARAVCVAPVSVGRWALVAAGAVVTKDVPDFALVVGVPARRIGWVGRAGRPLVAKGDGAWVCPETGAGFTETGGLLTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0S4</accession>
    <name>U5W0S4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH-type transcriptional regulator betI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362974.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21660"/>
    <dbReference type="EnsemblBacteria" id="AGZ42602">
        <property type="protein sequence ID" value="AGZ42602"/>
        <property type="gene ID" value="AFR_21660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4390"/>
    <dbReference type="eggNOG" id="COG3226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_21_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="178" mass="18729" checksum="6BDD86F857F7E9C1" modified="2014-01-22" version="1">MSPAKRDPEGRRRALASAAVEVIADSGVGRTTHRAVAARAGVPLGATTYYFPTLNDLVAAGLQQASDAMQAELDGWAGRLTAGNLAPTLVELSRGYLADRPRAILEYEVYLAAARDEQLRPLAQAWLDGLYSLLTPLTDPFTARAVAMLLDGALAQALATGSALDGATLQRAITRLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1C7</accession>
    <name>U5W1C7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362014.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17460"/>
    <dbReference type="EnsemblBacteria" id="AGZ41770">
        <property type="protein sequence ID" value="AGZ41770"/>
        <property type="gene ID" value="AFR_17460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3540"/>
    <dbReference type="eggNOG" id="ENOG50336JZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155188_1_0_11"/>
    <dbReference type="OrthoDB" id="1852398at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="104" mass="10966" checksum="F72BC8D28753EEF3" modified="2014-01-22" version="1">MDTVAGTWDLSVRTPIGTLAAVYVFTESEGVVSGTATGSGETVVLTDIGLTDTADGLRVTWRQAVTRPLRLNLDFEVIVRGAGLTGFSRAGRLPRSKVTGARQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W212</accession>
    <name>U5W212_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24415</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363739.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24415"/>
    <dbReference type="EnsemblBacteria" id="AGZ43149">
        <property type="protein sequence ID" value="AGZ43149"/>
        <property type="gene ID" value="AFR_24415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4946"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="27143" checksum="67745DCEFFEBD642" modified="2014-01-22" version="1">MSSAEAVVLDGVEHAYGDVTAVGPVSLTVPAGEFLVLVGASGCGKSTLLRLIAGFEQPTTGTVRTGGEAPVPGRGAGLVFQQPRLFPWKTVGGNVELALRYAGITDRGNRVEELLASVGLHDVAHRRTWQISGGQQQRVAIARALAVDNPLLLLDEPFAALDALTRERLQEDLRRVSADTGRTCVFVTHSVDEAVFLGSRVVVLSARPGRVALDLPIDLPRTGVAPDELRGLPAYAALRSEIGHAVRAAATVPEGVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W222</accession>
    <name>U5W222_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_24490</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24490"/>
    <dbReference type="EnsemblBacteria" id="AGZ43164">
        <property type="protein sequence ID" value="AGZ43164"/>
        <property type="gene ID" value="AFR_24490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4960"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="12"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="481" mass="50024" checksum="871DC35F8EF89495" modified="2014-01-22" version="1">MSAEQDTNRWKAMSVALVAGFMTLLDVSIVNVALPAIRADLDLSPGGLQWVLSGYALTFGLLLVPAGRFGDARGRRNLFIVGLALFTLASAAAGLATSALWLIIARLVQGAAAGVVNPQVSGLIQQLFKPAERGRPFGLLGATIGISTAVGPLLGGLLIQVFGAEQGWRWIFFINVPIGIAAIVLGYRWIPARRADQQDRQSLDSVGVLLLGLGVLLILLPLVQEREWQGPAKWLLLVAGLGVLGGFLAWERRYAAHGTPVIELGLFKQRSYGLGALIGLLYFAGFTTIFFIYTLYLQNGLGYSALLAGLAITPFALGSAGAAALGGRIVNRYGRPLVATGLTLVAVGLGGTVLALDLVRGTNGGWAAAFPLLIAGLGSGLVISPNQTLTLADVPVERAGSAGAVLQTGQRIGTAVGIAVVGAVFFNRLAAGNNDWSAALRMGLLVTIGLVLVALVAALADVVAARRDKTSEPVEGTLARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W251</accession>
    <name>U5W251_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363667.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43077">
        <property type="protein sequence ID" value="AGZ43077"/>
        <property type="gene ID" value="AFR_24045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4871"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_1_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="29935" checksum="BE26C02621670FBA" modified="2014-01-22" version="1">MPVRGDFAALLRAWRDRLQPAAVGIPAGIRRTSGLRREEVAMLAGISVDYLVRLEQGRSERPSEEVVASLARVLQLSDAERDQLFIAAGLPAPSPRTVPHHIPGSVQRLLARLPDVAVGVYTTHWTLLTANAAWVALLGPLDRARNLIVQQFTGTAVPVVQSAEQVEHFERALVSDLRTARLKYPDDPSVRDLVERLTGIDRFARLWAEGTIVEHRSKVKTFVHPQVGTVTLDCDVFTATGTDIRIVTYTAAPETEDASRFDLIRALGAAEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W283</accession>
    <name>U5W283_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362448.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19005"/>
    <dbReference type="EnsemblBacteria" id="AGZ42075">
        <property type="protein sequence ID" value="AGZ42075"/>
        <property type="gene ID" value="AFR_19005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3859"/>
    <dbReference type="eggNOG" id="COG3864">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038906_0_0_11"/>
    <dbReference type="OrthoDB" id="801007at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025154">
        <property type="entry name" value="Put_metallopeptidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018698">
        <property type="entry name" value="VWA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09967">
        <property type="entry name" value="DUF2201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13203">
        <property type="entry name" value="DUF2201_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2201_N" evidence="2">
        <location>
            <begin position="9"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2201" evidence="1">
        <location>
            <begin position="258"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="386" mass="41907" checksum="1E0910350F7C6197" modified="2014-01-22" version="1">MSGTLDRTKLLAARYRAAIDRPYLATALFSITVVESARVPTMGVDRRWRCYVSPAFVARTPVPELAAVWIHEVAHLLRDHHGRATRLPPGTGDDHYRVNVAQDCEINDDLVADRLPLPADRVEPRTYGLRAGLVFEKYLQLLPKEVRSVDCGSGAHGVDRPWEHDDPGGVSDVEAEAIRRDTARQLRSRGDVPGGWRRWADQLLESKVDWRRQLAGAVREAVAWASGAVDYAYQRPSRRSAALRGVILPSLRRPVPRVAIVVDTSGSMGEPELAAALAEVAAVLRVVGVGGNRTTVLACDAAVQVTRSVYSAADVQLAGGGGTDLRVGVAEALRSRPHVVIVLTDGFTPWPDAPLERTRLIAGLIGAQAPEPPGWITAVRVDPTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W295</accession>
    <name>U5W295_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19090"/>
    <dbReference type="EnsemblBacteria" id="AGZ42090">
        <property type="protein sequence ID" value="AGZ42090"/>
        <property type="gene ID" value="AFR_19090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3876"/>
    <dbReference type="HOGENOM" id="CLU_965177_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="25895" checksum="B6B2B26BC7972E16" modified="2014-01-22" version="1">MRLRQRRRTGGSSTGVNRKAIGGAVIAGVLVVGGVTGLQYASAGEQKKASAIIVVEGQNFDVAGCEKLEINAGAVVCDGEQLAPEEEIGAADAAAASAQALEDSCDQFAIDQAAADEEAAGAGEEDAAGEEGAAEDEAAAPAAPADMSKKAKKAVAKKWAKGMGKEKGAEQKEEAGAGAAEEEGAGAEEGAAQEDLDAAVTDARAALLVACLDLSGKKAAAAEAAGDAGAGEEAGAGEEAGAGEEAGAGDEAGAGEEQEAPAKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2K9</accession>
    <name>U5W2K9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTP cyclohydrolase-2</fullName>
            <ecNumber evidence="5">3.5.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP cyclohydrolase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ribA</name>
        <name evidence="7" type="ORF">AFR_19715</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 3 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362587.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19715"/>
    <dbReference type="EnsemblBacteria" id="AGZ42215">
        <property type="protein sequence ID" value="AGZ42215"/>
        <property type="gene ID" value="AFR_19715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4000"/>
    <dbReference type="eggNOG" id="COG0807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_2_1_11"/>
    <dbReference type="OrthoDB" id="900513at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00179">
        <property type="entry name" value="RibA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydro2" evidence="6">
        <location>
            <begin position="31"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="108"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="23139" checksum="D3E12F43897CD26C" modified="2014-01-22" version="1">MTEALGLGTIPKATVRTRVTVPLRFADGYSTTARVLTFDGLVDGKEHLALGLGDWERAVQATPLVRPHSECLTGDVFGSQRCDCGPQLREAVERITDTGGLLLYLRQEGRGIGLYAKLDAYALQEAGLDTYEANVALGRGEDERDYTAAAQMLLALGAGRIRLLSNNPDKAAQLEARGVRIAERIPTGVHLSSANLRYLATKATHTSHTLDLPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Q1</accession>
    <name>U5W2Q1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative antibiotic biosynthesis monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362558.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19570"/>
    <dbReference type="EnsemblBacteria" id="AGZ42186">
        <property type="protein sequence ID" value="AGZ42186"/>
        <property type="gene ID" value="AFR_19570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3971"/>
    <dbReference type="eggNOG" id="COG1359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131496_7_0_11"/>
    <dbReference type="OrthoDB" id="1909149at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="2"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="108" mass="12156" checksum="E18E60F5AC05BEB2" modified="2014-01-22" version="1">MIFITAKFLVKAEDADRWPEISRAFTEATRAEPGCLWFEWSRSLDNPHEYVLVEAFRDGDAGGAHVGSAHFKQAQQELPPHLAATPKIVSQSVEQEDWSELGEMAVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2T5</accession>
    <name>U5W2T5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Family 1 extracellular solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363912.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25280"/>
    <dbReference type="EnsemblBacteria" id="AGZ43322">
        <property type="protein sequence ID" value="AGZ43322"/>
        <property type="gene ID" value="AFR_25280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5123"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_5_0_11"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="443" mass="48123" checksum="4F7FB226A83B794E" modified="2014-01-22" version="1">MHPDKTPAADARTTRGHRTATRRRLLAAALVIPLALGAAACGGDDSGDDGKTELSIFWWGAEGRAALTEKALELYTQRHPDVTFKKTWQANQGYFDKLATLTAGGNAPDIFQIDDNYIAEYAGRNVTLDLTPYSGDGKLDLTKFPPGLRDYGVVDGKLAGVAFGENTQGLVFNKTLLDKFELPAPTTGMSWEEHIAWAENVSKVAKVPGTQDASAIYQALWVWLRQQGKDLYKDKELGFTEQDITAWFDLWKGARDRGATPTPDVIHEGNATDISKQLVVTGKAATSWVWANQMPELKKNTADELGVVAYPGDPKSLWPRASMYFSVFRGTKHKDQAVDVLNFLANDPEAGKILGTDRGLPSNLDVRKLVADTVTDPNMKQTIAVQNDLGKNFGPSPAVPLKGHSKVRSELIRIAEEVQYGRQTPAQGAAAFVAAAKTAIGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3N3</accession>
    <name>U5W3N3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362952.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21550"/>
    <dbReference type="EnsemblBacteria" id="AGZ42580">
        <property type="protein sequence ID" value="AGZ42580"/>
        <property type="gene ID" value="AFR_21550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4370"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040638_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666048" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="94"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="499" mass="52783" checksum="AD11B2A75CA545E8" modified="2014-01-22" version="1">MKKQRFAAALAAVAVGVTGCAAAPMATAGARSTLTASAETVRALAECPELPGLPTARCGQITVPRDRSNPGKGTIDVGFALVPHTDTSQPGLGTIVPNPGGPGSSTIDLAGQLFAESLQPLLDRRDLLLIDTRGVGRSTPLSCAALDGPARVFAPLPQQRRLIGECGDQLGDTVSDYTSIAVADDVDDIRATLGLSRLDLLGISYGTYLMATYAQRYPDNVRTISLAGAYAINVDTTGEVNAVAFRRAVRLVCERTGRCDGDRVISDLAALMKRLRKHPEKLTVVHEGVKHRVTLDEWQMTSVAGKVYSNQPDTATELALAKAAAAARHGDLAPARALVRKSLLKSAEDYSYGPGLLSDAQSWAVTCHDYLRDFDYADPVAKRTRQYTSTQAKLDNADFRPFSAQVWVSRADYDTGACLRWPADATAQPPFPAGAAMPDVPVLVLSGDLDANTPIESGDAAAAQFPHATHKVITGAGHTPASTPEGVGEIIDFIRDAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3P8</accession>
    <name>U5W3P8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364542.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26905"/>
    <dbReference type="EnsemblBacteria" id="AGZ43642">
        <property type="protein sequence ID" value="AGZ43642"/>
        <property type="gene ID" value="AFR_26905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5452"/>
    <dbReference type="eggNOG" id="ENOG5030871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2327546_0_0_11"/>
    <dbReference type="OrthoDB" id="1961996at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="98" mass="10537" checksum="1B311E9A79846DC9" modified="2014-01-22" version="1">MPFGVVQEMPGVSEAEYRQVEKHLGPDRPPGLLAHVAGPTTTGGWRIINVWADEAAFRTFQSERLVRAAGLAAQEDGFDPAKAAHFSSVSVDGAEMPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3R5</accession>
    <name>U5W3R5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362992.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21750"/>
    <dbReference type="EnsemblBacteria" id="AGZ42620">
        <property type="protein sequence ID" value="AGZ42620"/>
        <property type="gene ID" value="AFR_21750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4407"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097480_0_0_11"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="20895" checksum="5512002ED3A8EB93" modified="2014-01-22" version="1">MESVAVIEDPAAAEVSLDPIRSRLLALLAVPGSATMLAAQVGLPRQKVNYHLRTLEQHGLVELVEERRRGNVTERVLQATAASYVISPTAMAAVAPDPARAPDRLSARWLLAVAARLVRDVGELLTGAAKAKQKVATFAIDGEVRFATAADRAAFAEELTVAVTQLVSRFHDEDAPGGRSHRLIVAVHPTVVRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4A7</accession>
    <name>U5W4A7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_22405</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363132.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22405"/>
    <dbReference type="EnsemblBacteria" id="AGZ42751">
        <property type="protein sequence ID" value="AGZ42751"/>
        <property type="gene ID" value="AFR_22405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4542"/>
    <dbReference type="eggNOG" id="COG0531">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_12_11"/>
    <dbReference type="OrthoDB" id="527053at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="454" mass="47937" checksum="CB3B80FE0E49C933" modified="2014-01-22" version="1">MADPGTQDSTEEREQPTLNRVIGPGLLLLFVVGDILGTGVYALTGKVAAEVGGAVWLPFLVAFVVALLTAFSYLELVTKYPRAAGAALYTHKAFGIHFLTFMVTFAVMCSGLTSASSAAKAFAGNFAEAFDLSLGEGLGLTAVAVGFMTLIALVNFRGVGESVKVNVLLTCVELTGLLIVICIGAWALGGGDGDLSRLTEFNSPPGESAFFSVTAATALAFFAMVGFEDSVNMAEETKDPVRIFPKVMLTGLCITGLIYVLVAISAVALVSPEDLSEGDAPLLKVVSAGAPGFPLTIFAFITMFAVANSALINMLMASRLLYGMANERVLPKILGKVHRTRRTPWVGIIFTTLIAFGLIWFADLTALGGTTALLLLCVFTIVNVAVLVLRRDRVEHKHFKAPTAIPVIGAVACAYLASPLSGRATADYKVAGILLVVGVVLWAITYFTRRRHTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4E0</accession>
    <name>U5W4E0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560390.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28960"/>
    <dbReference type="EnsemblBacteria" id="AGZ44053">
        <property type="protein sequence ID" value="AGZ44053"/>
        <property type="gene ID" value="AFR_28960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5868"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="11"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="153"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="228" mass="24800" checksum="A232201DCEEB2CB2" modified="2014-01-22" version="1">MTSTDAGRPLRVAIGEDDVLLREGVARILTDAGLEVVSRSGDADDLLHRTLAYRPDVVVADVHMPPGFTDDGLRAAMELRRRSPGIGVLILSQFCEPAYVMDLVGERPEGVGYLLKERVGDVTAFVDAVTRVAAGGSALDPEVVARMLGRRTREGPLQQLTPRELAVLAAMAEGLSNLGVAESLLISHASVEKHVTAIFRKLRIAPLDSENRRVQAVLTYLRTDDRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4M6</accession>
    <name>U5W4M6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transcriptional regulator ycf27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28505</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560299.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28505"/>
    <dbReference type="EnsemblBacteria" id="AGZ43962">
        <property type="protein sequence ID" value="AGZ43962"/>
        <property type="gene ID" value="AFR_28505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5779"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="23887" checksum="B88D80CF14986886" modified="2014-01-22" version="1">MRVLVAEDERVLADMVAKGLRRLSMAVDVVYDGESALEKLGINRYDVAVLDRDMPGRTGDEVCRHIAGSGSGTRVLMLTAAAGIRERVEGLGLGADDYLTKPFAFAELVARLQALSRRSVAALPPVLEQDGIVLDVTRHTASRDGLALSLSPKEYAVLHVLLRAEGRVVSAEDLLEQAWDENTDPFTNTVRTTVMTLRRKLGDPPVLHTVARIGYRLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4R3</accession>
    <name>U5W4R3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_29635</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29635"/>
    <dbReference type="EnsemblBacteria" id="AGZ44188">
        <property type="protein sequence ID" value="AGZ44188"/>
        <property type="gene ID" value="AFR_29635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6007"/>
    <dbReference type="eggNOG" id="COG1386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045647_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005234">
        <property type="entry name" value="ScpB_csome_segregation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298">
        <property type="entry name" value="PTHR34298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04079">
        <property type="entry name" value="SMC_ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00281">
        <property type="entry name" value="TIGR00281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="127"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="219"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="135"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="444" mass="46667" checksum="86E9021C23D300E6" modified="2014-01-22" version="1">MSDERPESLAAQAASWVPPWERTQRPPDQNYAAEQRAAAGESDDEARSEANLSDDDDDFDPAFEGPNPAPAELPPFPEETGGSPTVDAPTEVPGEEELDPTDVGVGPEVAIPGAIEEAAENEIGSAPVVDAPLPEDGDDAGDVFDAAADDDVADDDEVDLPFTEEVPGPEADEPEDGDGPAASLTTALPGTDDVGELSAEAAAAIGAADVDAAVARGEAVAGARPSSGIPAQGRRSKGARAASEATSTRTEEEAEVKPEPEPIADEELRAALEAILLVVDEPVAEMQLAQIMEQPTERVGVMLEDISARYTAAGHGFDLRRAAGGWRLYTRPEYAPYVERFVLDGQSVRLTQAALETLAVVAYKQPVTRSRISAIRGVNCDGVMRTLNTRGLIEECGTEGETGAFLYRTTALFLEKLGLNSVDQLPPLAPFLPDDVEEVLDASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W541</accession>
    <name>U5W541_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_30390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560675.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30390"/>
    <dbReference type="EnsemblBacteria" id="AGZ44338">
        <property type="protein sequence ID" value="AGZ44338"/>
        <property type="gene ID" value="AFR_30390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6153"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="31337" checksum="E7D0F147DE1E0CB1" modified="2014-01-22" version="1">MTATGFALRTDGVGKRYRKGWALRDCTLALPAGGVIALVGPNGAGKTTLLRLAVGLLAPSTGTVEVLGQDVTASTPQTLSRIGFLAQDHPLYKRFTVAEMLRFGRACNLRFDHGLAERRLAKLGIPLDRRTGTLSGGQQAQVALALALAKRPDLLVLDEPVASLDPLARHEFLQVLMGAVAEGGVTVLFSSHVVHELERVCDHLVVLNQGRVTLTGDLDTLLTEHRLLVGPRTATDLDRAGAVVEAVHSDRHTTLLVRDGALPAAPGWQTQPVGLEDLVLAYLRRPSEPEETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6D3</accession>
    <name>U5W6D3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Histidinol-phosphate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364294.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26270"/>
    <dbReference type="EnsemblBacteria" id="AGZ43516">
        <property type="protein sequence ID" value="AGZ43516"/>
        <property type="gene ID" value="AFR_26270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5324"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_4_0_11"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="26637" checksum="314F5CC84ABCEA7C" modified="2014-01-22" version="1">MQSDLELARTAVLAGAAVGLRYFAALADLPQERKTDGSVVTEADRAVEAAVREVLTAARPDDAILGEEGGATGAGERRWIIDPIDGTAQFVAGDNRWLVLLALEEAGEITTAVAAVPAQGRVWWASRGGGAFSADLDGTGEQRLHVGQATTALAGARLGAIPVPPNLTAADEAITAPLAAVASVVDWDVHAALLVANGELDLALQTRGQVWDFAATSLIVSEAGGSYGGADGIRGPRAGVSLFAANPALWHAAHAVLWPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6H6</accession>
    <name>U5W6H6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364401.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26540"/>
    <dbReference type="EnsemblBacteria" id="AGZ43570">
        <property type="protein sequence ID" value="AGZ43570"/>
        <property type="gene ID" value="AFR_26540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5380"/>
    <dbReference type="eggNOG" id="COG3576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1576692_0_0_11"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="23"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="18712" checksum="6820476509BF9A5A" modified="2014-01-22" version="1">MPVTNPWLAGPAPTERLDRDRLSERILNLLSSQNMCVLATTGPAGPLATPVRYFHLDFTLMFTAAAGSPKLRNIAGDPRVSVGVFAPLVGQASSRGAQIFGEARVLLPGDDDFATYWPAVRWQSDHVERSRSLDDPPQGPLVVIPAGRIVYTEHWLRRDGYAPRQVWKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6I6</accession>
    <name>U5W6I6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561103.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32530"/>
    <dbReference type="EnsemblBacteria" id="AGZ44764">
        <property type="protein sequence ID" value="AGZ44764"/>
        <property type="gene ID" value="AFR_32530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6583"/>
    <dbReference type="eggNOG" id="ENOG50348RI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1105289_0_0_11"/>
    <dbReference type="OrthoDB" id="2028156at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="27270" checksum="EE7A53D2B2BF0446" modified="2014-01-22" version="1">MATFAALVLGLGICLLVVAGLVQLLMLGDLHAVRTIRRTTPSPAASWRAGTGRIAAEGIVDDGPAGPQSGPLSAARCSWYRVRLVREPSRSWSDEVHEDVLLDFSSPAPPTFSDHSGRVVLDPRLFEDTSGPEPRVTETTTVVHRSAAPADLPPFVPRPQIDDLRRGEKLTLTETRIPYAREVYALALPAGEPLLLMPNPQGLTVFTTDTREQVLAARRDSIATARWMTRIFVLAGLALIVISSAALWTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6M0</accession>
    <name>U5W6M0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364383.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26495"/>
    <dbReference type="EnsemblBacteria" id="AGZ43561">
        <property type="protein sequence ID" value="AGZ43561"/>
        <property type="gene ID" value="AFR_26495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5371"/>
    <dbReference type="HOGENOM" id="CLU_932658_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="30982" checksum="0C55084047308BC2" modified="2014-01-22" version="1">MITAAGFLRSAALAFGWIPAALLTAPVVLDASEYGGYRPDSDDLVMAVTVLAAIAGLCLGYAAARRRTAPLAGLVLIVVAAVVCFVANDAVYRWIVPLAQDLREGGWGPLNVLAVVVAAAAGFGLGLFRKPREAPLSRRLLLVAAALTAVAGLVVVPEFVRVGGELSTSTFVPPGTTLIDGGQRATVQLDAGRHALFGYFGSEPCDLGRKLSRPSVEFTDNSDSIVTELLGTFELPQAGPVTVVCGGRPGERFEVGDLPVIRGPLSHLVYAPVGVPLGLGLLPGALLAAWALRRPRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6V8</accession>
    <name>U5W6V8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561275.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33400"/>
    <dbReference type="EnsemblBacteria" id="AGZ44938">
        <property type="protein sequence ID" value="AGZ44938"/>
        <property type="gene ID" value="AFR_33400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6760"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_2_11"/>
    <dbReference type="OrthoDB" id="1617768at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="12079" checksum="0E231DD276E81B21" modified="2014-01-22" version="1">MDELSLVFGALADPTRRTIVERLARGNATVTELAELFAISQQAVSKHLKVLEHAQLISRTRSAQSRPCVLEAERLDAAVGWVAENKQVWADRHDRLEQHLARLRGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W739</accession>
    <name>U5W739_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclopropane fatty acid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFA/CMAS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561350.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33775"/>
    <dbReference type="EnsemblBacteria" id="AGZ45013">
        <property type="protein sequence ID" value="AGZ45013"/>
        <property type="gene ID" value="AFR_33775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6837"/>
    <dbReference type="eggNOG" id="COG2230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026434_0_2_11"/>
    <dbReference type="OrthoDB" id="1518440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003333">
        <property type="entry name" value="Mycolic_cyclopropane_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003085">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR003085-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="412" mass="45543" checksum="57AAA7B78892D247" modified="2014-01-22" version="1">MTTVLSPVADRIDPGRWPDVAATPRSRTRGVVAAAVFRRIVERLPLRVAYPGGAVTGSTGPTMRIHRPQAFFARLGADGLIGFGESYQAGDWDADDLVGVLTVFAQQMGTLVPPRLQWLRRFHGPRAPRAERNTVEGSRRNIHRHYDLSNDLFALFLDESMSYSSALFDRSQDFTLAQHRKIDRLLDATGVRSGTRVLEIGTGWGELAIRAARRGARVLSVTLSTEQRSLALRRAAEAGVADRVDVRLRDYREIEPVAGGYDAILSVEMIEAVGEAYWPTYAATLERHLAPGGSVGLQTITMADDRLAETRNTYTWIHKYVFPGGLVPSVPAIEAVLGRAGLAVRDRLDFGLDYAETLRRWRATFNARRGDVAALGFDATFLRTWNFYLAYCEAGFAAGYIDVSQLVSGRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7E8</accession>
    <name>U5W7E8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative PadR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561274.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33395"/>
    <dbReference type="EnsemblBacteria" id="AGZ44937">
        <property type="protein sequence ID" value="AGZ44937"/>
        <property type="gene ID" value="AFR_33395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6759"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089258_0_1_11"/>
    <dbReference type="OrthoDB" id="1629615at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="174" mass="19451" checksum="84F255ED41ACEC56" modified="2014-01-22" version="1">MTVPLTLLGLLEREPSHGYDLKRDYDAFFGRGKPLSFGQVYATLSRLARDGKVVISDVEPGEGPDRKRYIITDVGATEVDTWLKEPVAAEPHLQTVLFAKVTLALMLGRNAEAYLDTQRAAHLQRMRELTEIKRNGSLVDALLADHGLFHLEADLRWIELTSVRLESLAKAVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7M0</accession>
    <name>U5W7M0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acidPPc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45188">
        <property type="protein sequence ID" value="AGZ45188"/>
        <property type="gene ID" value="AFR_34660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7012"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072573_8_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="84"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="21842" checksum="F3D7FED54F12C72C" modified="2014-01-22" version="1">MLSVAAGVIAWLSPGGLGQSGAVTVKDGTSASLYLSLTGPVADAPSWLGTILEVASEGTLVVLGVLLVWSWWTGLRRRDARATAGAVLIGAGTIAAYAVSEAVKLVVDEERPCRALRAAADVIAACPEPGDWSFPSNHATLAAGLAAGLAILWPRLAAVTLPLAGAAALLRVLVGVHYPHDVLAGAILSSTVVAAVLLVFLPLAQRTVSRMIPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-02-10" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W864</accession>
    <name>U5W864_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Triacylglycerol lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="U5W864"/>
    <dbReference type="STRING" id="1246995.AFR_29250"/>
    <dbReference type="EnsemblBacteria" id="AGZ44111">
        <property type="protein sequence ID" value="AGZ44111"/>
        <property type="gene ID" value="AFR_29250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5927"/>
    <dbReference type="eggNOG" id="COG4188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052605_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665796" evidence="1">
        <location>
            <begin position="30"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="DLH" evidence="2">
        <location>
            <begin position="130"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="290" mass="30549" checksum="F217131FCC39902E" modified="2014-01-22" version="1">MFRGRTVAGAALAAALTAGSIALVPSADAATNPFERGPSPTTTSIEATRGSFAVSQTSVSSLSASGFGGGDIYYPTSTTAGTFGAVAIAPGYTARKSSMAWLAPRIASQGFVVFNIDTLTTSDQPASRGRQLLAALDYLTQRSTVRTRIDSSRLGVMGHSMGGGGTLEAADDRPALQAAIPLTPWNLTKLWPGVTVPTMVIGAENDSVAPVASHSERFYSSLPSTLNKAYLELNNASHFAPNSSNTTIAKYSIAWLKRFIDDDLRYDQFLCPAPRVSTAISEYRDTCPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8D0</accession>
    <name>U5W8D0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561598.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35030"/>
    <dbReference type="EnsemblBacteria" id="AGZ45262">
        <property type="protein sequence ID" value="AGZ45262"/>
        <property type="gene ID" value="AFR_35030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7088"/>
    <dbReference type="eggNOG" id="ENOG5030J0W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097505_0_0_11"/>
    <dbReference type="OrthoDB" id="1550125at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="20593" checksum="C8990B47CBA221BB" modified="2014-01-22" version="1">MRYRPPSGARTEPPPADDAAAWITGASPDGWFTEPPEVIVDRDEIIIWGRVPTPELSADATEADRAAAQAGRIHQFREDTRDDRIKVALQVEHRYQRKVSWGVRCGDTQDLFTHLSAPVMTRLRQPERQVLDTLVDAGVARSRSEALAWCVRLVGEHTEDWLTELRSAMTKVEDLRRQGPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8E1</accession>
    <name>U5W8E1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561745.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35765"/>
    <dbReference type="EnsemblBacteria" id="AGZ45409">
        <property type="protein sequence ID" value="AGZ45409"/>
        <property type="gene ID" value="AFR_35765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7239"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069431_0_0_11"/>
    <dbReference type="OrthoDB" id="1175268at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="27152" checksum="07689D100AE5A780" modified="2014-01-22" version="1">MELDIATAAERPDRTGLWSELDGFWPAFMTKDNNGAFFYGYETTQYPEYQLLAVDKETGRAVAKAHSVPVSFSGAIDDGLPEDGWDWAVRRAAHDRMRGVAPTFVSALEITVRPDLRGQGLSGRMLAAMRANVAARGFQDLVAPVRPTGAHGQPIDRYAYEQRPDGLPVDPWLRVHVRAGARIVNVAHSSMVMMGTLAQWREWTGLPFDKSGDVTVPYALGAVHCDVTQNHAVYVEPNVWVHHRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8N0</accession>
    <name>U5W8N0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="5">2.5.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Dimethylallyl diphosphate:tRNA dimethylallyltransferase</fullName>
            <shortName evidence="5">DMAPP:tRNA dimethylallyltransferase</shortName>
            <shortName evidence="5">DMATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Isopentenyl-diphosphate:tRNA isopentenyltransferase</fullName>
            <shortName evidence="5">IPP transferase</shortName>
            <shortName evidence="5">IPPT</shortName>
            <shortName evidence="5">IPTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">miaA</name>
        <name evidence="9" type="ORF">AFR_35555</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5 7">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5 6">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5 8">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35555"/>
    <dbReference type="EnsemblBacteria" id="AGZ45367">
        <property type="protein sequence ID" value="AGZ45367"/>
        <property type="gene ID" value="AFR_35555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7196"/>
    <dbReference type="eggNOG" id="COG0324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032616_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="PTHR11088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="5">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="5">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="5">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32869" checksum="4EDA0F741C97CEE0" modified="2014-01-22" version="1">MLPPPGVVAVVGPTAAGKSALSLALAHALGGEVVNADSMQLYRGMDIGTAKLRTDEREGVPHHVLDVWDVSEPASVQAYQALARAAVDDIAARGKVPLLVGGSGLYVRAVLEEFEFPGTDPAIRDRLERELASEGTFFLYERLRELDPVAAEKILPTNGRRIVRALEVIELTGAPFTAALPSPTPHYQSVQIGVDRDTAELDQRIADRVDLMWAAGLLDEVRGLDGLREGRTAGRALGYQQALAQLDGDLTEDQAKDETVRATRRFVRRQRSWFRRDPRITWLDGASGTLTEDALAACAAVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8T9</accession>
    <name>U5W8T9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560745.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30750"/>
    <dbReference type="EnsemblBacteria" id="AGZ44410">
        <property type="protein sequence ID" value="AGZ44410"/>
        <property type="gene ID" value="AFR_30750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6225"/>
    <dbReference type="eggNOG" id="COG2223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046685_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="425" mass="43547" checksum="46767772932EE4D3" modified="2014-01-22" version="1">MSALTAPQVRRRFLWLIALRWLPVGLMIPITVLLPLDRGLTLAQYGSAAALQGILVLLLELPTGGLSDAIGRRPVLLLAGVVEIGALTLLTFADTVVLFLIFYTLQGVYRALDSGPLDAWYVDQSLAADPGADIETGLSHSGVVLGVAVAAGALLTGGIVALGPYGPVAALTMPVFVALGLQVVSFAAVVLLMTEHRPRRGLVALAESVRGVPAAIGGALGLLRRSRIIVALVAVEIFWGFGMVAFESLMPVRLAEVVGNTDHASALMGPVGSAGWLASALGAALIPLLSRRIGAPWTGFALRILQGVTVVGMALFAGPVGVIAAYLVCYTVHGASSPVHMGLLHRQVEGPYRASLISLNSMVAQPAGALGLVTLTAIASAATLPVALLVGAVMLALAAPLYLVARGNSPAPAPATPPAEMTPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W978</accession>
    <name>U5W978_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_37520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45758">
        <property type="protein sequence ID" value="AGZ45758"/>
        <property type="gene ID" value="AFR_37520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7589"/>
    <dbReference type="eggNOG" id="ENOG5033HJG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076587_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20300" checksum="999F5E84507D22D3" modified="2014-01-22" version="1">MSMFSRLFGGGGRSVAPLPYPPMSPEGLAARWVRWVAGIGPVRNPVGDDSGLYADINQPDDVWFLAGTFGDNAERSCPVPAGRPLFFPAFNMWHWPAQGPPSSMPHAFGTLLVDGRPYELDVIATPIPFEVAGAALNPVTRTKARIPTTVWGIWRKLDPLPPGPHVVQFSGGDGHGFTVNVTYHLMVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9F2</accession>
    <name>U5W9F2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_36965</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36965"/>
    <dbReference type="EnsemblBacteria" id="AGZ45647">
        <property type="protein sequence ID" value="AGZ45647"/>
        <property type="gene ID" value="AFR_36965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7478"/>
    <dbReference type="eggNOG" id="COG1408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037730_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="3">
        <location>
            <begin position="238"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="526" mass="54916" checksum="7A446B65EB8E26EC" modified="2014-01-22" version="1">MATAAARRLWARPWARRLRVWAGVVAVSLTGVVVGTMLFAQSEIAVGPFRADMSISPSIEGGTEVDIPPLGSLHLDSHDGPIHLKVDLGSLDQGRTEALIDDPTAISAAGQSAVDDVTKGVLQLGLRALGAAIVCSLILSALIFRNIRRVASAGVLALVVTGGSLGLAVATIRPDSISEPRYEGLLVNAPAVVGDARRIADNYGRYADQLQELVSNVSRIYTTVSALPVFDPAGGTTRILHVSDLHLNPTSWGVIRTVVKQWDIDAVVDTGDIVDWGSSAETSYVNSIGQLGVPYVYVRGNHDSTAIQAAVARQTNAKVLDNDVVTINGLTIAGIGDPEFTPDKSEAPAANADDPGSSPLLRSGEELATTIRASKTKVGVALVHDPAMAPPLSGVVPLVLAGHLHKREVRTLDQPSPDPSASPAPSASAGTQAPLETRLLVEGSTGGAGLRGLENEEPTPLTMSVLYFDESQSLKAYDDIQLGGTGEQNVTMQRKVIGTDPEPTTNPSVFNGVTPGLTPNSAPPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9P7</accession>
    <name>U5W9P7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561032.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32210"/>
    <dbReference type="EnsemblBacteria" id="AGZ44700">
        <property type="protein sequence ID" value="AGZ44700"/>
        <property type="gene ID" value="AFR_32210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6520"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065561_0_0_11"/>
    <dbReference type="OrthoDB" id="489714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_5" evidence="1">
        <location>
            <begin position="73"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="353" mass="38250" checksum="ABE487E321F2CB7C" modified="2014-01-22" version="1">MTALRPHAEDQYAEELALLAGTDDRPRPPGWRLSPQAVVTYLLGDGAKITPKYVGPRRLMEVAVSTLVTDRALLLLGVPGTAKTWVSEHLAAAISGDSTLLVQGTAGTAEEAIRYGWNYARLLADGPSEAAIVPSPIMRAMQTGTIARMEELTRVPSDVQDALITVLSEKTLPVPELNTEVQAQRGFNLIATANDRDRGVNELSSALRRRFNTVVLPMPASADDEVEIVARRVAQLGRSLALPEVPTALEEIRRVVTVFRELRSGLTEDGRTKLKSPTGTLSTAEAISVITSGMALAAHFGDGRLHPSDVASGIVGAVIKDPVSDSVVWREYLETVVRDRPDWRDFYRAARDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA50</accession>
    <name>U5WA50_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562237.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38240"/>
    <dbReference type="EnsemblBacteria" id="AGZ45902">
        <property type="protein sequence ID" value="AGZ45902"/>
        <property type="gene ID" value="AFR_38240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7736"/>
    <dbReference type="eggNOG" id="COG4102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032896_3_1_11"/>
    <dbReference type="OrthoDB" id="663130at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010869">
        <property type="entry name" value="DUF1501"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07394">
        <property type="entry name" value="DUF1501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666455" evidence="1">
        <location>
            <begin position="41"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="417" mass="42240" checksum="94B80896C130EA34" modified="2014-01-22" version="1">MSVVNCGCAPVSRRKLLAAGAAGAFAGLVGEQLSTQLAFAADNSYAGDVLVLLSLRGGFDGLSAIVPAGDAGYYRARPGIAVPKSQLIGGDAYFGLNPALAPMLPFWRAGTFGAVQAVGQSAPNRSHFSAMEELERAAPGTTVRTGWLDRMMGGLGADSPLDAVSVGSAMPARVLAGPAPNLGLAAIDDFVLSGEDRTRPMAATMAALYAGAPEALAATTAMTTGALATTRTLKTAGYTPANGAVYPDTDLGAALRDVARLVKADIGLMSATVDSGDWDMHENLGTAAPGKKMYDHLADLAEALAAFATDLGAAGLARVTLVTVSEFGRRVAQNGSGGLDHGYGNTMLVLGGGVRGGKVHGRWPGLAAAQLVDGDLAVTTDYRAVIGEILRARCGVTDVAGVFPGVRPSSLGLVAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAE2</accession>
    <name>U5WAE2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562413.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39125"/>
    <dbReference type="EnsemblBacteria" id="AGZ46079">
        <property type="protein sequence ID" value="AGZ46079"/>
        <property type="gene ID" value="AFR_39125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7918"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762700_0_0_11"/>
    <dbReference type="OrthoDB" id="889006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="357" mass="39993" checksum="CAE2BD6A8665920A" modified="2014-01-22" version="1">MADDGRRLGARLRALRESGFSNQPVTQGVVATALGRSVPLVSSWEKGKAQPSEEWLNAYARFFASPRSIEDGHPRLLTEEDLREEELDRFEGLFRELLQLRVAGSGTPPVPSNADELTTRSPWEGMWHFPDQASITLVCARLPAELRASEIYSSPASPDYVELYSYSDLDALMELYGHVYAANPGVTVRRKLADELTQDDVTSHLVLMGGVDWNPMTRRVTQSLNLPVAQDRREGNDPDVGSFRVLDHNGQATVFRSKLEGDRLTEDVAQFCRAPNPFNRKRSVTICNGNYGRGTYAAVRTLTDPRFRDRNRAYIEQRYTPDDTYSILARVSIVAGEVITPDWTMAGTVLHEWSNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAG2</accession>
    <name>U5WAG2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33610"/>
    <dbReference type="EnsemblBacteria" id="AGZ44980">
        <property type="protein sequence ID" value="AGZ44980"/>
        <property type="gene ID" value="AFR_33610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6802"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052530_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015814">
        <property type="entry name" value="Pgluconate_DH_NAD-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09130">
        <property type="entry name" value="DUF1932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1932" evidence="2">
        <location>
            <begin position="176"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="27194" checksum="156D5E96556B3C6C" modified="2014-01-22" version="1">MGAGLGKSLREGGARVVATVAGRSERTRRLAEGLELLPSLAEVVSAADVVLSVTPPGSALAAARSIADAARSTGSSPVVADLNAISPATMAAVAEACAGLPVVDGSISGPPPSTRPGARIYLSGVRATDVAGLPWNSQVEPIVLGTELGSASALKMCTAGVYKGLNALVTQAMRTAGRYGVLDQVLADLARNDLDGSAGVAVSATKAHRFVDEMREIAATQAGAGLTPALFEALAAVYAEVATTVLAAGDPESADRSLSPAEIVRRLSPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAV7</accession>
    <name>U5WAV7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein, fmdb family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40150"/>
    <dbReference type="EnsemblBacteria" id="AGZ46284">
        <property type="protein sequence ID" value="AGZ46284"/>
        <property type="gene ID" value="AFR_40150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40150"/>
    <dbReference type="eggNOG" id="COG2331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136025_4_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013429">
        <property type="entry name" value="Regulatory_FmdB_Zinc_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09723">
        <property type="entry name" value="Zn-ribbon_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00834">
        <property type="entry name" value="CxxC_CXXC_SSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02605">
        <property type="entry name" value="CxxC_CxxC_SSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxxC_CXXC_SSSS" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="73" mass="7248" checksum="4E990351A7F97A55" modified="2014-01-22" version="1">MPRYDYRCRACGDTFEMNRPMAESAAPAACPQGHADTVKLLSTVAVTGRGGAMPPAPARAAGGGGCCGGACGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBN4</accession>
    <name>U5WBN4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate-binding protein PstS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_40765</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of the ABC transporter complex PstSACB involved in phosphate import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562739.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40765"/>
    <dbReference type="EnsemblBacteria" id="AGZ46407">
        <property type="protein sequence ID" value="AGZ46407"/>
        <property type="gene ID" value="AFR_40765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8252"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034528_0_0_11"/>
    <dbReference type="OrthoDB" id="1222928at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665764" description="Phosphate-binding protein PstS" evidence="5">
        <location>
            <begin position="32"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="6">
        <location>
            <begin position="39"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="4">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="4">
        <location>
            <begin position="188"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR002756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR002756-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="363" mass="36671" checksum="0C36044D625CD49B" modified="2014-01-22" version="1">MKLQRYYVAGIALTAALALTACGSDNTDSPAAGGASIAPGSCTAGQLTAQGSSAQKNAMDEWIKAYQGQCADTKIGYEGTGSGAGIQAFIAGTADFAGSDSALKEEEQPQADAKCVGGQALNLPMVIGPVAVVYNLDGVDGLQLSSATIAKIFAGTIKKWNDPAIAGENSGAKLPDTAINAVHRSDESGTTDNFTKYLSKTAESDWTFGNAKAWKAPGGTGAAKSDGVAGLVKSTVGAISYVELSFAENSDLKMAKIKNGAGEYTELSAESAGKTIAGAKVKGTGDDLALDIDYATTEAGAYPIVLVTYEIACSKGSAKAAAIKGFLTYTASTGGQSALAELGYAPLPEAVRAKVEASVAKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBP3</accession>
    <name>U5WBP3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35600"/>
    <dbReference type="EnsemblBacteria" id="AGZ45376">
        <property type="protein sequence ID" value="AGZ45376"/>
        <property type="gene ID" value="AFR_35600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7204"/>
    <dbReference type="eggNOG" id="ENOG5031GUS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140155_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="13430" checksum="1CD6AE5664FC0DB8" modified="2014-01-22" version="1">MTTLDVPVDGAVLCDVWHVDGDFPTLIECYLAPADLAEATIASAVSVRLGAELLLPDDTLNPSRYVLAEPDGTLRAVHVDEIETDDGTERRHLRPCTGDDPACALGPGCGRSRWKPVPTPERPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBQ5</accession>
    <name>U5WBQ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562949.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46618">
        <property type="protein sequence ID" value="AGZ46618"/>
        <property type="gene ID" value="AFR_41820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8463"/>
    <dbReference type="HOGENOM" id="CLU_2646304_0_0_11"/>
    <dbReference type="OrthoDB" id="2193438at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="76" mass="8520" checksum="50CCEC84029E4C34" modified="2014-01-22" version="1">MTDTEIKFDDETAVEPAPELIATHYLASIDELVEHLRAADQLGLGVRVTSYLVAEEPRWELELLTASPTHQEDEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCH6</accession>
    <name>U5WCH6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type multidrug transport system, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_36825</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36825"/>
    <dbReference type="EnsemblBacteria" id="AGZ45621">
        <property type="protein sequence ID" value="AGZ45621"/>
        <property type="gene ID" value="AFR_36825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7450"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="260"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="31018" checksum="11DD09775E38C621" modified="2014-01-22" version="1">MLHIENLTKVYRRGARANDGISLDVAAGEVLGLLGHNGAGKTTLLSQIVGLVRPTGGHITLLGRDPVADPAYARKVCSFQPQAQAPLTGVTARQAIEIVGRIRGGKRRVVQRRTAELLAALDIEEWADQPGERLSGGVRRLTAFSMAIVEPGGLVMLDEPTNDVDPVRRRLLWEQVRALATEDRAVVLVTHNITEAERAIDRVVVLSQGRVTAAGTPASLATDQEMRLEVWLPGADPAPPLWARTVRRDGDRLVLSLAAADGPSRPPGPTACRRVFRSTPPASRTSTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCJ1</accession>
    <name>U5WCJ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell envelope-related transcriptional attenuator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_37330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37330"/>
    <dbReference type="EnsemblBacteria" id="AGZ45720">
        <property type="protein sequence ID" value="AGZ45720"/>
        <property type="gene ID" value="AFR_37330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7552"/>
    <dbReference type="eggNOG" id="COG1316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016455_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LytR_cpsA_psr" evidence="3">
        <location>
            <begin position="92"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="377" mass="41077" checksum="70C6617CCED3FA47" modified="2014-01-22" version="1">MAKSSEPKTKRRAPLWARLCAIFGCVLMVVSGGALVTSQVLVSRYAGAVETKDLFGNAANGATKAPVSDIKGPLNILLVGIDPRNDTTAPLSDSIIVVHIPKGLNQAYLFSIPRDLRVEIPPFQKTGFRGGTSKINAAMSYGSSVGNGKHDVAQGFDLLAKTVSKLTGIKKFDAGAIINFNGFKDIVDAMGGVTMTIDQNVKSEHLQPNGKPRPRRPECASNNCEHPYVGPQATYKKGTYHLEGWQALDYVRQRYGLPRSDYDRQRHQQQFIKAMASQALSKNVITNPIKLDSVLRAAGDALIFDGNGHTVVDWGLALKGLRADDMTLVKLPGRSDIQNGRYLGESLESSADDFFKSVDNDSIATFLIEHPEFFQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDL4</accession>
    <name>U5WDL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562424.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39180"/>
    <dbReference type="EnsemblBacteria" id="AGZ46090">
        <property type="protein sequence ID" value="AGZ46090"/>
        <property type="gene ID" value="AFR_39180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7928"/>
    <dbReference type="HOGENOM" id="CLU_1438272_0_0_11"/>
    <dbReference type="OrthoDB" id="1718855at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="19637" checksum="019A27205D89C0C4" modified="2014-01-22" version="1">MSIPPDEVEAPADDAVLFRSSPWRTFALLSGAVLLAYVAVSPLVELLMGAPDDPWWSTVAQGAVIGALVAGGYAVSARSGLSTWVRASSGGLELAAQGSDPVLLAWPDIDHVVVRRDGFRTVLDVTPVDLDRVHPVSEEHEGWPTIRDDDADPSGRPAFTADLTQIWPGPRVLRRELARRMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE41</accession>
    <name>U5WE41_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_40205</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40205"/>
    <dbReference type="EnsemblBacteria" id="AGZ46295">
        <property type="protein sequence ID" value="AGZ46295"/>
        <property type="gene ID" value="AFR_40205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8137"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014298">
        <property type="entry name" value="RNA_pol_sigma-X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02952">
        <property type="entry name" value="Sig70_famx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="149"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="252"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="313" mass="34206" checksum="28BED258761B7908" modified="2014-01-22" version="1">MYAGDPYSRDVFAARLALTEGLSALRRGIDDSIAHAIRSDGPKRTRNRPHINESPSRHLPPTGNAKPGSGRVAVPGRPQAPQQAGPDRRPGTPEADSTVVVPTQSTTGPPSESEPPKYPARPDPGDAAAEVWGLVERAQAGESAAFGLIYDRYVDTVFRFVYFRVGNRQLAEDLTSDTFLRALKRIGSFTWQGRDLGAWLVTIARNLVADHFKSGRYRLEVTTGDVLDADREDRGPEGSPEAAVVDHITNVALLTAVKQLNPEQQECIVLRFLQGFSVAETARTMGKNEGAIKALQYRAVRALARLLPEGFQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEH5</accession>
    <name>U5WEH5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562777.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40955"/>
    <dbReference type="EnsemblBacteria" id="AGZ46445">
        <property type="protein sequence ID" value="AGZ46445"/>
        <property type="gene ID" value="AFR_40955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8291"/>
    <dbReference type="eggNOG" id="COG0718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149293_0_0_11"/>
    <dbReference type="OrthoDB" id="1969833at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="15026" checksum="9C075D39F598A6F1" modified="2014-01-22" version="1">MQTGTGNSDPLLEPGEALDRLAAWKGRVDQLAVSTKSMSDQLAQLRVTAADDTKTVQVTVDAQGALVDIRFGRRIQQIEPDAVARTVMSTIRLARQQIAERAENIISETVGTDSAAGRAIAERVVHRLQDQRTGEDSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF77</accession>
    <name>U5WF77_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase I</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_41535</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41535"/>
    <dbReference type="EnsemblBacteria" id="AGZ46561">
        <property type="protein sequence ID" value="AGZ46561"/>
        <property type="gene ID" value="AFR_41535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8404"/>
    <dbReference type="eggNOG" id="COG0302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049768_3_3_11"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035998">
        <property type="term" value="P:7,8-dihydroneopterin 3'-triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="PTHR11109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP_cyclohydroI" evidence="6">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="16442" checksum="5091110A2FE01839" modified="2014-01-22" version="1">MFAGLRVDPAKVLTTTFEANHEELVLVRDIELMSLCEHHLLPFKGVAHVGYIPGVDGRITGLSKLARLVEVFARRPQVQERLTSQIADLLMEKLSPRGVIVVLECEHLCMEMRGIRKVGARTVTSAVRGAFQRDGKVRAEAMALINAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNT3</accession>
    <name>U5VNT3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357397.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00825"/>
    <dbReference type="EnsemblBacteria" id="AGZ38454">
        <property type="protein sequence ID" value="AGZ38454"/>
        <property type="gene ID" value="AFR_00825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.165"/>
    <dbReference type="HOGENOM" id="CLU_175281_0_0_11"/>
    <dbReference type="OrthoDB" id="1941802at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="105" mass="11778" checksum="0BEFBDB61A116BDC" modified="2014-01-22" version="1">MLKFKNAEEQAAWNMAEALSEKGFSCMRQAEEAAENFRSGKMQMRRNFKARGMSEVDADIRWSGTTAAKKALSDNGWYMSQATMYNEAAAAQYAKALYLKNSDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPL3</accession>
    <name>U5VPL3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nicotinamide riboside transporter pnuC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02315</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nicotinamide ribonucleoside (NR) uptake permease (TC 4.B.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357695.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02315"/>
    <dbReference type="EnsemblBacteria" id="AGZ38752">
        <property type="protein sequence ID" value="AGZ38752"/>
        <property type="gene ID" value="AFR_02315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.468"/>
    <dbReference type="eggNOG" id="COG3201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076589_2_0_11"/>
    <dbReference type="OrthoDB" id="1301128at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034257">
        <property type="term" value="F:nicotinamide riboside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006419">
        <property type="entry name" value="NMN_transpt_PnuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36122">
        <property type="entry name" value="PTHR36122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04973">
        <property type="entry name" value="NMN_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01528">
        <property type="entry name" value="NMN_trans_PnuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="22602" checksum="C049AE99A29586BB" modified="2014-01-22" version="1">MIDWLSSVAFTTFGVGTTWAEVLGFATGLVNVGLLVRQHILNWPVGILNVLLLMLVFWSAGLYADAGLQIVYVILGFYGWWAWLYGGARHTRLVVRSTTRHEWLLLAVAGVLLTGVMWFFLDRFTGSTVPLADAVTTALSLLATYGQTRKLVESWWLWIAADLIYIPLYGYKDLWLTAILYVAFLILCILGLRAWRAAERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ51</accession>
    <name>U5VQ51_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03770"/>
    <dbReference type="EnsemblBacteria" id="AGZ39043">
        <property type="protein sequence ID" value="AGZ39043"/>
        <property type="gene ID" value="AFR_03770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.761"/>
    <dbReference type="HOGENOM" id="CLU_1821233_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="141" mass="16290" checksum="74EEFE265BF3F333" modified="2014-01-22" version="1">MPAVATVRAVSSPIHHLTVIRYFRDGRHWESTDILSPSWPDVVTAIERMDDFCYPIVQLNCTADEEDETIFNVIGGDGSYALFHQMGEWEYTDPNGSDEEVRLWQSDQGYFCKRRNVLTDLDEVLRLTRIFYETGSYESLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQA7</accession>
    <name>U5VQA7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative heme oxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_04095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358053.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04095"/>
    <dbReference type="EnsemblBacteria" id="AGZ39108">
        <property type="protein sequence ID" value="AGZ39108"/>
        <property type="gene ID" value="AFR_04095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.827"/>
    <dbReference type="eggNOG" id="COG5398">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057050_2_0_11"/>
    <dbReference type="OrthoDB" id="1371367at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004392">
        <property type="term" value="F:heme oxygenase (decyclizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006788">
        <property type="term" value="P:heme oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19165">
        <property type="entry name" value="HemeO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002051">
        <property type="entry name" value="Haem_Oase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016053">
        <property type="entry name" value="Haem_Oase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10720">
        <property type="entry name" value="PTHR10720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01126">
        <property type="entry name" value="Heme_oxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000343">
        <property type="entry name" value="Haem_Oase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00088">
        <property type="entry name" value="HAEMOXYGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="SSF48613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000343-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000343-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="23649" checksum="F3737AFAE4886372" modified="2014-01-22" version="1">MTLADPMEKLSVRLRTGTRMDHDAAQGSGFLDRLAAGELPRQAYADLATQHWFIYETLEQAAAVMATDSVAGSFVFPELTRLPALTDDLETLLGPGPQLTPLPATTEYCARLRAVAFDHPWGFVAHHYTRYLGDLSGGQYLGPAIAKAYALNGAGHRFFVFEGVSPPAFRTRYRELLDQAAFAPDDEQKFLAEVTEAYRLNIAVLRELKERWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQQ6</accession>
    <name>U5VQQ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_04190</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358072.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04190"/>
    <dbReference type="EnsemblBacteria" id="AGZ39127">
        <property type="protein sequence ID" value="AGZ39127"/>
        <property type="gene ID" value="AFR_04190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.846"/>
    <dbReference type="eggNOG" id="COG2211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027408_6_0_11"/>
    <dbReference type="OrthoDB" id="1249292at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039672">
        <property type="entry name" value="MFS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328">
        <property type="entry name" value="PTHR11328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="16"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="439" mass="44978" checksum="EB6DA3020AAE0D89" modified="2014-01-22" version="1">MTVAGPSQDVRLPRRVVAGYSLGSLVTGPFGTVPGLLLLPYLTDTLGVTAGVAGLLVLLPKAWDVLVNPIAGRISDRSGDRRAFLLGAGLALGVLFAAIFAGPFATGAAAGAYVAVAFLAAATAFAFFQVPFVAMPAEITSGYAERTRLMTWRVAVLALAILASGALAPLVVDLGGGGLTGHRWSGAFVGALIVVGTLGVFAGTRTAPSRSPAEQEPSLRAQLRIAAANRSFRVLLLCYIVQAAGIGTMLAGVQYFADHIIDQDAGATFLFAAFVGPALLVMPVWSRVGARHGKLRGLIAASLLFAAGALALLLSPALPAVPIYLIVALVGCGYAGQQVFSLAMLPDLIEQDTVRTGRRQAGVFTGLWTAGETLGLALGPGIYALILQLFGYVSSSTGEAAAQDSTARLGVLLGFTLVPALLVGAAVTLLRRFSPPDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR02</accession>
    <name>U5VR02_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_05570</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358934.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05570"/>
    <dbReference type="EnsemblBacteria" id="AGZ39403">
        <property type="protein sequence ID" value="AGZ39403"/>
        <property type="gene ID" value="AFR_05570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1130"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_11"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="11"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="125"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="232"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="384" mass="41481" checksum="B2B752FC7F9D3A46" modified="2014-01-22" version="1">MPDFDVYQLPEDHETIRAAVREVCDARVAPNAAEADESGEFPKASYDALRSSDFHAPHIPVEYGGAGADALATAIVIEEVARACASSSLIPAVNKLGTMPLILAGSESLKQRYLTPVASGEAMFSYCLSEPEAGSDAVSMTTRAVRDGDYWVLNGVKRWITNAGVSEYYTVFAVTDPAARSRGISAFVVEKSDEGVSFGAPEKKLGIKGSPTREVYFDNVRIPLDRMIGDEGTGFATAMKTLDHTRVTIAAQAVGIAQGALDFAKGYVKERKQFGKAVADFQGIQFMLADMGMKLEAARQLTYVAAGKSERGDADLTYFGAAAKCFASDAAMEITTDAVQLLGGYGYTRDYPVERMMRDAKITQIYEGTNQVQRVVMARQMLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRP7</accession>
    <name>U5VRP7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YceI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357275.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00210"/>
    <dbReference type="EnsemblBacteria" id="AGZ38331">
        <property type="protein sequence ID" value="AGZ38331"/>
        <property type="gene ID" value="AFR_00210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.43"/>
    <dbReference type="eggNOG" id="COG2353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071003_3_0_11"/>
    <dbReference type="OrthoDB" id="1767227at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007372">
        <property type="entry name" value="Lipid/polyisoprenoid-bd_YceI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036761">
        <property type="entry name" value="TTHA0802/YceI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04264">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00867">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101874">
        <property type="entry name" value="SSF101874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YceI" evidence="1">
        <location>
            <begin position="21"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="20839" checksum="CB3F174ABA4B50A0" modified="2014-01-22" version="1">MTDSVVSTREFEGLQIPAAGTYQLDAAHKRVGFVVRHLMVSKVRGNFSEADATIIIAEDPLQSSVTATITTASVQTGTVDRDNHLRTGDFFEAEKYPVMTFRSTGIKSHAGAEFVLDGELTIKDVTKPVELVVEFEGATTSPYGQAVFGFSATTEIDREDWGLTYNMALESGGVMIGKKVKIEIEGEAIRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS19</accession>
    <name>U5VS19_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359113.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06465"/>
    <dbReference type="EnsemblBacteria" id="AGZ39582">
        <property type="protein sequence ID" value="AGZ39582"/>
        <property type="gene ID" value="AFR_06465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1311"/>
    <dbReference type="eggNOG" id="ENOG503263F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108887_1_0_11"/>
    <dbReference type="OrthoDB" id="1776279at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="17321" checksum="82D78CF9078FB16F" modified="2014-01-22" version="1">MTVTVRTADVPDAVLALAGLDPIHYAEAFRFETPAIRSPLEWSRLILEGAPPIKRLQMRAVWTGLGIRLAMPFSSGQVLGWRIRQSGADAVVLGVRAAIGLTARLVIRAEPGQVTQSMLVRIDRDRARGVWDRLAPRHRHFLTELLDRDDLRAPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSP1</accession>
    <name>U5VSP1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GTPase Era</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 13" type="primary">era</name>
        <name evidence="13" type="ORF">AFR_07975</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07975"/>
    <dbReference type="EnsemblBacteria" id="AGZ39884">
        <property type="protein sequence ID" value="AGZ39884"/>
        <property type="gene ID" value="AFR_07975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1628"/>
    <dbReference type="eggNOG" id="COG1159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038009_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00367">
        <property type="entry name" value="GTPase_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTP-bd_Era"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="PTHR42698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00436">
        <property type="entry name" value="era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51713">
        <property type="entry name" value="G_ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Era-type G" evidence="12">
        <location>
            <begin position="90"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="11">
        <location>
            <begin position="288"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="8">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="8">
        <location>
            <begin position="124"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="8">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="8">
        <location>
            <begin position="207"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="8">
        <location>
            <begin position="236"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="145"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="207"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ39884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="378" mass="41648" checksum="FC83B75907C6F965" modified="2014-01-22" version="1">MTAGERNELRRQERRAARREQSDPGAAKRGAAEGVTKRPAPKQTATSAPAAPVSQDPRPSGITAPARTERKKHRHKAASRSVEVDTTTYRAGFACFVGRPNAGKSTLTNAIIGQKIAITSNKPQTTRHVIRGILHQENSQLVLVDTPGLHRPRTLLGERLNDLVRETWSEVDVIGICFPADEPVGAGDRFISAEMAELKATVIAVVTKVDLVDPATLTKRLLDVSKLYDFADIVPVSAVSGHQVETLVDVLVKHLPESPPLYPDDVLTEEPEQVLIGELIREAALEGVRDELPHSIAVLVEEMLVEGRTTKIYANLFVERDSQKSIVIGTKGARLKDVGTRARAEIEQLLGRKIYLDLHVRIAKEWQRDPKQLRKLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT05</accession>
    <name>U5VT05_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative diguanylate cyclase/phosphodiesterase with GAF sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357803.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02855"/>
    <dbReference type="EnsemblBacteria" id="AGZ38860">
        <property type="protein sequence ID" value="AGZ38860"/>
        <property type="gene ID" value="AFR_02855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.573"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_34_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="353"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="1">
        <location>
            <begin position="495"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="757" mass="81754" checksum="0606D220716D98D1" modified="2014-01-22" version="1">MGMLATQQLTEFLSAVADRPDGPAAQFAAVECAARALEAEVAVLVIDDCVVAALGVPAEQVRPEVLAEVAAGRRSTLMLAGTAYAVTGAPIGGQTPGVLVLGRTGPDFSAEDVCLLRGMARVLELSLQALTVVERERLQVEENDRLTDSLQERQRLFEHLTRIQRLIARRAPLQHILDAITAGAAELLDLELASLNLLEKDDPSVGSMVSSVGFPPDVLPRMKRVPLPSAGVAGLAVLGDELVMVEDYANSPIAIPEVVRSKMKAVMAVPVHENGEVVGCLTVGTSTGPREWDKPAQEVLRAFAEHVSLALTDARTLEAMNEAFHDSLTGLASRALFQARMERAFVTAEHENAGVAMLFVDLDRFKVVNDSLGHAAGDKLLIAVADRVRGCLRSADMAARLGGDEFAVLLPLTHGVDEAVPVARRILEAMREPFELGGKETFISCSIGVAYSVAGGHEAQELMVSADLAMYQAKKQGKDRFEIFEPALQAVFQAGLQLEADLRRAVVRHEFELRYQPIVHLRTGEITGLEALVRWQHPERGIIPPLDFIPLAEETGMIVPIGEWVLREACRQAAQWNLRRVGEKPLTVSVNLSAVQLDQADLPQRVASALEDSGLPAECLVIELTESLLVDHRPSTLHQLEQIKSLGARLAIDDFGTGYSSLAYLRRFPVDIIKIDKSFVDDVGDEPTAAALTLGIIQLGQALHLSTIAEGIEDLEQLNELTDGNCELGQGYYFAEPLTDDDMSELLFPAAGGSVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT72</accession>
    <name>U5VT72_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_08615</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359543.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08615"/>
    <dbReference type="EnsemblBacteria" id="AGZ40012">
        <property type="protein sequence ID" value="AGZ40012"/>
        <property type="gene ID" value="AFR_08615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1753"/>
    <dbReference type="eggNOG" id="COG1064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_20_1_11"/>
    <dbReference type="OrthoDB" id="969875at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="11"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="35223" checksum="E8588CCFF8C7FFB2" modified="2014-01-22" version="1">MTTYRAIEVTGARRFALVDRELREPGPGHVRIRAEACGVCHTDVLAVEGLRADPSTPIVPGHEVVGVIDAVGPGVTHWRPGERVGVGFLGGQCGECDRCRRGDFVNCTDQPRTGTTVDGGYAEIVYARTSGLVRVPEGLTSTEAAPLLCAGLTTLQALRRIDARPGARVAIQGIGGLGHLALQYAARLGYRVVAVARGTEKEALARRLGADEYVDSSAQDVAAALQALGGADAIVATAANGASMSALVPGLAPRGVMVVVGAATDPIEIDTNDLIFGTRTVTGSLTGSSIDNEDTLAFSVRQGVRPMIEVLPLGEAARAYERMMAGAARFRLVLDLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT94</accession>
    <name>U5VT94_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">PTS system mannitol-specific transporter subunit IIC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_02960</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02960"/>
    <dbReference type="EnsemblBacteria" id="AGZ38881">
        <property type="protein sequence ID" value="AGZ38881"/>
        <property type="gene ID" value="AFR_02960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.595"/>
    <dbReference type="eggNOG" id="COG2213">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028721_2_0_11"/>
    <dbReference type="OrthoDB" id="554815at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013014">
        <property type="entry name" value="PTS_EIIC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004718">
        <property type="entry name" value="PTS_IIC_mtl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00851">
        <property type="entry name" value="mtlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51104">
        <property type="entry name" value="PTS_EIIC_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-2" evidence="10">
        <location>
            <begin position="21"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ38881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="355" mass="36592" checksum="11D6931F101BEC7A" modified="2014-01-22" version="1">MAELYAPAAQDSGVKARVQRFGGYLAGMVMPNIAAFIAWGLITALFIPSGWLPNEKFAALVDPMVHVLLPILIGYTGGRMVHGQRGAVVGAVATIGIVVGSDVPMFFGAMIVGPLAAYALQLVDKLTIDRIRPGFEMLIDNFTAGIVGGLAALAGVVAIGPVMRIFTNAAGNGVEWLVTHHLLPGAALLIEPAKVLFLNNAINHGVLGPLGVAESAETGKSILFMLETNPGPGFGILLAYLLFGPSALRPSVPAAMAIQFLGGIHEIYFPYVLMKPSMVLAAIAGGGAGILTFMLTGAGLVATPSPGSIFAYLAETPRGGYFGVLAGIVVAAGVSFVIGSLLLGFGRLTRDEEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT97</accession>
    <name>U5VT97_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative sodium/proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_03330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03330"/>
    <dbReference type="EnsemblBacteria" id="AGZ38955">
        <property type="protein sequence ID" value="AGZ38955"/>
        <property type="gene ID" value="AFR_03330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.670"/>
    <dbReference type="eggNOG" id="COG0025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_8_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004705">
        <property type="entry name" value="Cation/H_exchanger_CPA1_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="PTHR10110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00831">
        <property type="entry name" value="a_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0050">Antiport</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0915">Sodium</keyword>
    <keyword evidence="5 6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="7">
        <location>
            <begin position="9"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="550" mass="59046" checksum="8F749155B9DB5378" modified="2014-01-22" version="1">MNVETTLLFILACVAVVVAIHWFAHKTGLPAAALLTLIGILYAVLPGPNIALEPELILTLVLPPLLYSAALDSSLIDIRHNLRTVISLSVVLVLLTALLIGLGFSLFVAGATLAAGVAVGAAVAPPDPVAALSVGKRVGLPRNIVTLVQGEGLLNDATALTLLTVAVAAAVGEGFSFYAAVGEFVLAATGGLVAGIVVAYAVRAARPIMRDPLIANAISLATPFVAYLLAEQVHVSGVLAVVVAGLIVGHDAPRLSSGASRLQVSAVWRLVDFLLEGLVFLLIGQQLPAVVKGLTEYKTSTIIIAVAVSVGVVLLLRPLFLILTQMLPQSLHTRLGGTNEDASAPARTRERRLNGKEITALSWSGTRGVITLAAIFTVPEFTESGAPFPDRDLLLFCAFVVVLVTLVGQGVTFAPLVRMLGLRANEADNARLRNEARSASVQAALDRLDEIDHETHGELEDKAIETMRKQLGHRMERYQRRLDLLDKSDSDEVPVSPQYEAALRIRRSTIDAQREELLRWRDANRLSDENLRVLERELDHEERLLPDRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTP7</accession>
    <name>U5VTP7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_09750</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09750"/>
    <dbReference type="EnsemblBacteria" id="AGZ40239">
        <property type="protein sequence ID" value="AGZ40239"/>
        <property type="gene ID" value="AFR_09750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1988"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665752" evidence="7">
        <location>
            <begin position="25"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="449"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ40239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="491" mass="49270" checksum="7F00EE8FF9CAEEE1" modified="2014-01-22" version="1">MLCVMALMIVLDSTIVAVAVPAIQADLGFSPAGVAWVVNGYLIGFAGLLLLAGRLGDLVGARRVFLAGLVVFTAASLLCGLATTAPLLVAGRFVQGVGGALASAVIFSLIVRLYPEPGAQARAIGVYSFTQAGGAAFGFVAGGLLTEAAGWPWIFLVNVPIGVVVLVAALRVVPRETGLGLGAGLDVSGATLITAGLSLGVYAIVDGGPWYGLAAFLLVTAFLIRERYARSPLAPLPVLRRRRLLGTGAAVVLIFATGMGFQFVNALFLQRVAGLDAIGTGLAFVPTPLVIGVMSLIVAPRVTARFGARPVLLAGLGVLAAGLLLLVPVPADPSYATDILPALLIMGLGIGVVIPAIIMLAMSNAAPQETGLVSGLTNTAQQAGAALGLAVLAVIAARVTESSLASGVSEVAALRDGYSRAYLTAAGFAAAAFVIAALALRRPATIPTDDPAVTPAPPTSAAATDPAVTPAPPADAAAAERQTCALVADQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU20</accession>
    <name>U5VU20_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acyl_transf_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360418.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11020"/>
    <dbReference type="EnsemblBacteria" id="AGZ40493">
        <property type="protein sequence ID" value="AGZ40493"/>
        <property type="gene ID" value="AFR_11020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2245"/>
    <dbReference type="eggNOG" id="COG1835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039835_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl_transf_3" evidence="2">
        <location>
            <begin position="8"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="38435" checksum="92CCD928E10F28FB" modified="2014-01-22" version="1">MTGDGRDRSVDAVRAFAVLGVVAGHWLVTGLVPDADGLHTVSPLVAMPGAAPVSWVLQTLGLFFFAGGFAAARSRTPVRLGRYAWPVLGLLAVWAPLLLLAAAFDAPAATLHTIAGLVVSPLWFLLPYLALSLATPALQRILHRTGPVLLVVPIAVVALVPGLVAVPAAWAVPWLLGMLVAGYTRTSDRNTMLAYTGAALAFGGAAAMVALIRYGHFPASAVGIPGAERSNLSPPSLVAVALAVTQTGVFLLLRGPILRLMRHDRVWRPVAAINRAAAPIYLTHQSVLLAVAGVAALVNPAMPGLLTAPTGPSWAAGRALWLPVLALVLAAATRGRHHGEPSFFKRGLRGTLFDGSRARTTDGGDRCAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUT6</accession>
    <name>U5VUT6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative signaling protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_06055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06055"/>
    <dbReference type="EnsemblBacteria" id="AGZ39500">
        <property type="protein sequence ID" value="AGZ39500"/>
        <property type="gene ID" value="AFR_06055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1230"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_49_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="164"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="306"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="562" mass="60919" checksum="9F911A64A23B393A" modified="2014-01-22" version="1">MGRTGKTMDRIRRGVDDTMIFTSLLLLAWPAMFDDVVRGDAGVPGLIIGIATVFFLGVGVILLAGRWPLLQYAPVASAVIMAGFWFGRNDTISPELTWLIIAVAAIILIRQFLGARTNEGLVRDLTRQRAQLARQAFRDPLTELGNRKLFMDHATDALADADDTMTAVILFDLDGFKEVNDTYGHATGDELLRTAAERLTANVRTNDTVSRLGGDEFVVLLPRLVDDQIADTVANRILRDLSQPLVIGDTVLTIPASAGIAITRGSGMAVDDVIREADLALYQAKADGKGVARRFDPAQFAEAEKRRREESDLRRALDAGEFEVHYQPIVDLDGERTVGVEALVRWNHPERGLLPPAAFLEMAEALGLLPRLGGWVLSEACRQAAEWQRQEPGFELNVNLSASQLGNPDLIDEVRAVLESTGLPPAQLVLELTESVALVDLVESARILGALKTLGVRIALDDFGTGFSSLSHLGTLPVDVVKIDKSFVQAMQESSGASVAEAVLQIARTFNLAPVAEGVEDAGQASRLRELECAQAQGYHFARPMPAGELTDLLDRQSALSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUZ9</accession>
    <name>U5VUZ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360743.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11985"/>
    <dbReference type="EnsemblBacteria" id="AGZ40684">
        <property type="protein sequence ID" value="AGZ40684"/>
        <property type="gene ID" value="AFR_11985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2440"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_2_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="23"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="29287" checksum="93148BC1167590E5" modified="2014-01-22" version="1">MTENLSIAGNTLAYEVSGQGPLVVLAHGIGDSRHSYRFLAPALVDAGYRVANVDIRGCGDSGLGWDGYSRTDIAGDLVAVVRHLGGPAVIIGQSISGGAATIAAATAPDLINGVIELAPFTRVQPFDFGGLVRVKRFRSGYTQMAQVMVRGSLSSWKKYLDAAYPVKPADWDAELARIEAKLSEPGRMKVLQAMCKTSPTDAGAQLANVTCPVLVIEGSLDPDWADPRAEGEKILADLPRGLGELVVIEGAGHYAHVQTPDQVLALARPFLGKTLANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV38</accession>
    <name>U5VV38_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360721.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11865"/>
    <dbReference type="EnsemblBacteria" id="AGZ40662">
        <property type="protein sequence ID" value="AGZ40662"/>
        <property type="gene ID" value="AFR_11865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2416"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049704_1_1_11"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="211"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="34941" checksum="CE8A8B8D085CC44E" modified="2014-01-22" version="1">MALLLDTRNLPPRESADAVYATLTSPSAPASVRLSPGIRSLIEGWPIGAGMTLLSNDSSGGLHMQRTARHVRADSPERLSLTLDTRGICDARQFQTIVRRRNELHLLDLTSVYESRWRGAFAATAFLADYTALGFPVDVARAVIPLVRTSPLHDLTMRHLRDLPAIARRTAPGPALGMLGVATADLVRALIASVAPGNPHSRAAHARSLRTVVLAYIDAHLHEPGLTPARVAAEHKVSLRYLYQLFADEAESPAEAIWQRRLEGARRELAHRATHHILISATARRWGFTDPRHFARRFRAAYGISPGDWVRMHLGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVC5</accession>
    <name>U5VVC5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361017.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13360"/>
    <dbReference type="EnsemblBacteria" id="AGZ40958">
        <property type="protein sequence ID" value="AGZ40958"/>
        <property type="gene ID" value="AFR_13360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2713"/>
    <dbReference type="eggNOG" id="ENOG502ZACP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1293291_0_0_11"/>
    <dbReference type="OrthoDB" id="2030038at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="21996" checksum="2B4C0D4CEECD2681" modified="2014-01-22" version="1">MSLVLQPLAILREHRRAFVVLAVLCPVMFLVGVLVTAVIPELRPSGLGGLQADSGAPGLGTLIADAYRSENVAWAAAVTLGVNLVSASFLQTTLPSLVIPFIGVVITVVRAFSWGILFSPLGRPDSSYLVHYVTLLIEGGAYLIVGFAAWVQARRFLQPQRFGRPNRRAGYLDGLAATAKLYVWVVVLLVIGAVYEAFSVIHLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVG0</accession>
    <name>U5VVG0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coproporphyrinogen 3 oxidase, aerobic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360918.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12865"/>
    <dbReference type="EnsemblBacteria" id="AGZ40859">
        <property type="protein sequence ID" value="AGZ40859"/>
        <property type="gene ID" value="AFR_12865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2613"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_4_0_11"/>
    <dbReference type="OrthoDB" id="1706427at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="8"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="115" mass="12543" checksum="9CCE84AE8FBAD604" modified="2014-01-22" version="1">MREPTFWILTALATESRHGYALIQEADRLSDGRVKLQAGTLYAALDRLVDEGLVEPDRDEIVRGRARRYYRLTNEGATALDAEAARLQASVNAANGQLSARRFGLGQATAGLVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW03</accession>
    <name>U5VW03_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_14330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14330"/>
    <dbReference type="EnsemblBacteria" id="AGZ41148">
        <property type="protein sequence ID" value="AGZ41148"/>
        <property type="gene ID" value="AFR_14330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2908"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_666710_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024655">
        <property type="entry name" value="Unchr_glyco_hydro_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11790">
        <property type="entry name" value="Glyco_hydro_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665773" evidence="2">
        <location>
            <begin position="31"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="3">
        <location>
            <begin position="41"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="422" mass="44225" checksum="25895E2EAD7449E5" modified="2014-01-22" version="1">MRSRRRVLAVAVSATVVAGLGVLGITTAMAADAGPIKGLGGKCVDVAGANSANGTAVQLWDCNGTGAQRWTVDGQQIQALGKCLDVTAASAADGAKVQLYACNNSGAQKWSAQGGRLVNAGSGKCLDATGQSSASGTRLQIWTCTGAAGQTWQVPGATTAPTTAAPTATQPPATQPPATGVKKKGVSSWEFAGVAGGVKDVGAGWYYNWGTNNDKMPADAEFVPMIWDETVVTAANLAKVKTEGTTLLGFNEPDLNEQAKMPVQQALDLWPQLQGTGMRLGSPAVAYGGDTPGGWLDQFMTGARQRNLKVDFITLHWYGSDFSDAAVGQFMGYVKAVHDRYQLPIWVTEFGLMNFSGTPKFPNTAQITAFIRNATAQLQSAPYVERYAWFSLPAVGDSVDYGLYRDATTPTEAGKAYRSAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW70</accession>
    <name>U5VW70_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Maltose or maltodextrin ABC transporter, permease protein MalF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_14780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14780"/>
    <dbReference type="EnsemblBacteria" id="AGZ41238">
        <property type="protein sequence ID" value="AGZ41238"/>
        <property type="gene ID" value="AFR_14780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3001"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="59"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="32487" checksum="BF7E9DB3ED366566" modified="2014-01-22" version="1">MVAFLSPALVLFALLVLTPIVIAAYSSFFEWNGFGVPTTFVGLDNFHRLLTDEIFAGDLRRALLLVVLSLAIQLPVSLALAMLLNQPLRGRRAYRMLFFTPYVLSEVVTAVLFTMIFSPNQGLANHVTRLIGLGDLGATWLADPDTVMYSLFLVISWKYFGFHTILYLAGRQGIPPELHDAAAIDGAGPWQAFRHITLPLLGPTIRISVFLSILGTIQLFDMIWVLTGGGPIRSSETLAVTMFQEGFKRFHVGYASAISMAIFLISLTFGLLYQRFVLRRDLEGAITTIGDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWG1</accession>
    <name>U5VWG1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359532.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40001">
        <property type="protein sequence ID" value="AGZ40001"/>
        <property type="gene ID" value="AFR_08560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1741"/>
    <dbReference type="eggNOG" id="ENOG5030W37">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1003368_0_0_11"/>
    <dbReference type="OrthoDB" id="1950683at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="29030" checksum="76439B0DF679E765" modified="2014-01-22" version="1">MSTEYPQFATGYAQYPPAQDQYAAPVSAPPVTDQGEAFPPVAYPQEGAADYPEPVAYPQLTVPAGYPGEVPEASGLPQAGAYPPPSAYPTAPPWAAASPEPEPAPSYPGQAVTGHGGLLVPFPDEMQHASRAQPPAVWPVAIFTLFFGILGAFSASRRAGAARRGRNSTAPYWITYLVTLAAAGFVWFVMSAVVILPLVDSIQESSRLSAVQDAVVNDGQLKGANIDVTSAQCRAISDRNAAGMREYLCRLTLGDGHTGSLMLTADTDGHWTSPPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXA1</accession>
    <name>U5VXA1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16110"/>
    <dbReference type="EnsemblBacteria" id="AGZ41504">
        <property type="protein sequence ID" value="AGZ41504"/>
        <property type="gene ID" value="AFR_16110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3269"/>
    <dbReference type="HOGENOM" id="CLU_1648491_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665843" evidence="1">
        <location>
            <begin position="20"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="160" mass="16818" checksum="A89F1EDB9461AD0F" modified="2014-01-22" version="1">MRRAVQALLISGVLFPAAACGTAASTPSASVSEAPAVVTAADLSTRAACESLSQVYSRNLAPLAQSLNEMVAARTSAGADKEQERKVKQSLTNFATALELATHDSNDTAVRTDGEKTADQLRARAGDAKFLRTIKTAEDAQKVMGPTLKEWLAPMAKHCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXI1</accession>
    <name>U5VXI1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">8-amino-7-oxononanoate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17000"/>
    <dbReference type="EnsemblBacteria" id="AGZ41678">
        <property type="protein sequence ID" value="AGZ41678"/>
        <property type="gene ID" value="AFR_17000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3450"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_12_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="48"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="419" mass="45128" checksum="6F990F6197DD05E7" modified="2014-01-22" version="1">MNPILLSGLPALSPGQQGIAELHDAGLWDQVIEEIDGRRIRVGDHWMTDFASCNYLGLDLDPQVQASVGEQIARWGTHPSWSRMLGSPRLYPQLEERLAELLGAPDVLTLPTISQIHLSAIPALAGKGGTILLDSRAHRTIYDGCVYARAQGATVKRFASGDLEQAAELLRQAPADQPRLVCMDGVNSMTGNHPDLTAFAELCREHGALLYVDDAHGMGVIGERRPDETSPYGARGNAVVKHTGGTYENIVLVGGLSKSYSSLLAFIACEPAVKRHLKITCPPYLYSGPVPTASLATAHAGLDVNAERGDALRAHLHHLTARVLDQLDAFGVKTPNESGFPLIEVPLRDPALLQPVGRALLDRGAYVTLAPYPGVPRDEVGFRIQLTAANTDAEVDALLDTLKWLHHDFSDAGSPMRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXP6</accession>
    <name>U5VXP6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10760"/>
    <dbReference type="EnsemblBacteria" id="AGZ40441">
        <property type="protein sequence ID" value="AGZ40441"/>
        <property type="gene ID" value="AFR_10760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2193"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055817_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043917">
        <property type="entry name" value="DUF5753"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19054">
        <property type="entry name" value="DUF5753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5753" evidence="1">
        <location>
            <begin position="67"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="256" mass="28534" checksum="5ACF5EBDEDBA26AA" modified="2014-01-22" version="1">MIRRTHWSVSKLHRIETGAVTVQPVDLEALLRMYGVRDRGQVAELIDLALISRRRQWWSSYRLDPEYREFVAYESEASRITVYQALLIPGLLQTELYAKAATASIGKKSDDTDVQNAIDVRMRRQAELFGRIAGSRPPELVALLDGAVLERPIGGADVMRAQLDHLVEVARQPHVRLVVVPLRHGARPGPGGLFELLQFRGTADPDVVFVQSPVKDFVVKDPDATAAYHDIVDAIVGSGLTRDAAVEEIRRIRDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXS3</accession>
    <name>U5VXS3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Tyramine oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_16955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16955"/>
    <dbReference type="EnsemblBacteria" id="AGZ41669">
        <property type="protein sequence ID" value="AGZ41669"/>
        <property type="gene ID" value="AFR_16955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3441"/>
    <dbReference type="eggNOG" id="COG0405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="426" mass="42431" checksum="A8581C23E94547A9" modified="2014-01-22" version="1">MAGPAAVLSLGAAAAAADPDALRAAGTTVMPDTGPLTITVPGLVAGWAAVASLGARLGWADRLAPAIALAAGGAAVSPGLGRALREGRDTISADPGLRAVFGDAGEGTVVRQPALAASLTELAGDWTTFYRGPLGQRLAAGLTRLGSPLTAADLAAHRAEITDPLLRYAHGAEWAAAPPPSQGATFLALVGSGRLLTDARRAQAARDALLGDPRTGPVDLPGLLLDAPRPAAGAATGPIPTGDTVAVTAVDADGTAVSLIQSLFHSFGSGLLEPDTGIVLHNRGSMFSLDPRHPGRLVPGSRPPHTLCPVVAIDGDTVLALGCQGGRSQPWILAQLAADALRGEDPQAIVDRPRWIIGPGGLVLEPGTPGAGDLTAAAGDLPVTTTAALHDSGGHVQLARLRGTTLDAASDPRADGVAAVLGTLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXS8</accession>
    <name>U5VXS8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative dienelactone hydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361637.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16400"/>
    <dbReference type="EnsemblBacteria" id="AGZ41562">
        <property type="protein sequence ID" value="AGZ41562"/>
        <property type="gene ID" value="AFR_16400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3326"/>
    <dbReference type="eggNOG" id="COG0412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054590_1_0_11"/>
    <dbReference type="OrthoDB" id="1889569at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DLH" evidence="1">
        <location>
            <begin position="18"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25789" checksum="B52E44F7F5FB2872" modified="2014-01-22" version="1">MKVRENEVQVASPGGDIRSIVISPVGDGPWPGLLLYTDIFQLTESTLRTARRLAASGFVVCVPEIYPHGELAGVALEFDDAGKQAGLGGAAATTAAQFDADRVAVLDFMEQRSDITTLFATGFCIGGHLAFRAAFDPRVAATVCFYPTGVHNGALGADADAGSLARAADIRGRLMIVFGSQDPHVPADARLQILSALYAAGHDDLELHVYSGGEHAFMRDVGARHDPELTDLALTEAVSFLHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXV9</accession>
    <name>U5VXV9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361678.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16620"/>
    <dbReference type="EnsemblBacteria" id="AGZ41602">
        <property type="protein sequence ID" value="AGZ41602"/>
        <property type="gene ID" value="AFR_16620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3370"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_11"/>
    <dbReference type="OrthoDB" id="384611at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="2">
        <location>
            <begin position="12"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="468" mass="46779" checksum="BC2497721DE8D3F3" modified="2014-01-22" version="1">MYETRHLIAGAWTTGDSSRHLTVLDPADGSPVTRCAIAGPAEVGAAVDAARAAAPGWARTSPGARAAALHAAANAVEAATGELAAIMSAEMGKPVGGAADSIAAGVGTLRQYAELGPTHRGKALAGNDDAIDLMAYGPRGVVAVITPWNDPVAVSCGLIGAALVTGNTVVHKPSERTPATGHRLAELLAPHFPDAVLSLLSGDGETGAALSGAPVDVVAHVGSTATGRAIAAACARTGAKALLENGGSDPLVVDADVDPVWAAGQAALGAFANSGQICTSVERIYVHADQAEAFSAALVTEAEKWAGEIGPLVDTRLRDAVHAQVQAAAGAGARILTGGSVPEGPGAHYPPTVLTGCTDDMAVVREETFGPVAAVVTVDSFDVALERAADSSYGLAATVLTASMSHAQRAWRELAAGTVKINAVFGGAPGGAAQPRRASGDGFGYGPELLDELTVLKAVHLEAPPGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY53</accession>
    <name>U5VY53_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361899.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17170"/>
    <dbReference type="EnsemblBacteria" id="AGZ41712">
        <property type="protein sequence ID" value="AGZ41712"/>
        <property type="gene ID" value="AFR_17170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3483"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_11"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="255" mass="27269" checksum="9644A4E425BE9574" modified="2014-01-22" version="1">MTVLDLFRLDGKVAVVTGGNRGIGKAIAIALAEAGASVVVAARDAERNEQAVAELSKIGPAALAVTTDVTSRSDLTALRDTVTERLGPVDVLINNAGVGIHGESLTIDDESWQRVMATNLDAVWKASQVFGEQMVARRSGSIVNVGSMSGMIINRPQWHAPYAVSKAGVHHLTRSLAAEWAPHRVRVNAIAPGYTKTEISDIDAPEFKHYWIDEVPMQRYAQSSEISPSALYLASDASTFVTGSVLVIDGGYTLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYF5</accession>
    <name>U5VYF5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362097.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17670"/>
    <dbReference type="EnsemblBacteria" id="AGZ41812">
        <property type="protein sequence ID" value="AGZ41812"/>
        <property type="gene ID" value="AFR_17670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3582"/>
    <dbReference type="eggNOG" id="COG4249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016759_0_0_11"/>
    <dbReference type="OrthoDB" id="601140at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="795"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="855" mass="90658" checksum="A81BAD17F91E9EDF" modified="2014-01-22" version="1">MTPKTISARARRRARYTGETYAQARDAVLRLTAAGATALPEAHNAAQQRLESRIMSAILSRPAPAGLLPVRAVRPFGDGIELLITEGLTEPFCGDVFAPLDGFTARPRGHAIEVSDAASSARVLLRNLSLRRYRTAVAAGSDEPASVDPARQGEVSAMLRRLALLDEPDVAGWASDWFRWVARLSAEPARQPGEQLVAMLADPLFGCAVSGVDGLENVRRRRLAPFRAPAPRRRRVFALMVGIDRYLGPMPALRGCRNDIEAAISHLRGIIPDDLHVVALYDEQATRAAVLAGFHDHLGQAGQGDSVLFWFSGSGSQAQSPLDPGPVQTLVCSDSRVDDVPDLYAPELTRLVDDIAGRGAHVVTVLDACHSGGLSARPSIVRSPEELAPDLPLGSLPGLLREDGRTAVRPEHVALAACRADETAQEMVIDGATRGLFSSALLSALTRLGPGATYRELMIAARAQVEDRIEHQVPTMSPVDGRLIDQPFLGGPVRRPATRAVMRRVREQWEIDLGACHGFAADGDGPTLVAVAGSGPLRQAQVLEVQVAKSLVAPVGWEPDPGAQYPVALAAVPRPAVSLAFDNTAGAAAAGLRAAIETAGPGGRPSPYLRVVETGPPDVIVSEADGLTALDGNTGKPLGILPDQDPVRELERLSQWLQLRRLDNPGSPLAGAVKIELISGGGGRLPVGADGTVELAYRFAGDGWQSPEVRISLRNTTDRRLYCILVDLAGEARSHVDLFPGGWVDPGKVAAAMRGRQIRVSLPQSRPVMPGATATDWLKLLVAEQPLDAEKYLLPRTGQGGRAGESRLSRPATAAPVSGSLVGHRDVAAAYRGDLGGGDWAVTTLRLITSVPVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYJ8</accession>
    <name>U5VYJ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360790.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12225"/>
    <dbReference type="EnsemblBacteria" id="AGZ40731">
        <property type="protein sequence ID" value="AGZ40731"/>
        <property type="gene ID" value="AFR_12225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2485"/>
    <dbReference type="HOGENOM" id="CLU_1567343_0_0_11"/>
    <dbReference type="OrthoDB" id="2150192at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665877" evidence="1">
        <location>
            <begin position="29"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="17444" checksum="8D7D89646753C303" modified="2014-01-22" version="1">MKKPLGVAVVVIGLAAAASLNSIGVASAGQQPVPAMVISTPVASVAVPAPPAIKDSDPSLTFRQNGRIVTITRRSTPVATVTLTSATWSAHSATAVLTVTATRPIIVDPAMFTLYDAEGWENEAEQSKPVRFGAGTAALTLTFTGTPARPEALGWVAKPDGDAVAVWERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYU3</accession>
    <name>U5VYU3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protocatechuate 3,4-dioxygenase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_19280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362500.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19280"/>
    <dbReference type="EnsemblBacteria" id="AGZ42128">
        <property type="protein sequence ID" value="AGZ42128"/>
        <property type="gene ID" value="AFR_19280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3916"/>
    <dbReference type="eggNOG" id="COG3485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027719_5_0_11"/>
    <dbReference type="OrthoDB" id="1456634at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018578">
        <property type="term" value="F:protocatechuate 3,4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019619">
        <property type="term" value="P:3,4-dihydroxybenzoate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024756">
        <property type="entry name" value="PCDO_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012785">
        <property type="entry name" value="Protocat_dOase_b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12391">
        <property type="entry name" value="PCDO_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="SSF49482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02422">
        <property type="entry name" value="protocat_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00083">
        <property type="entry name" value="INTRADIOL_DIOXYGENAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INTRADIOL_DIOXYGENAS" evidence="4">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="24592" checksum="83664AC651CCC2FC" modified="2014-01-22" version="1">MSAHPPLLSPGYKSTILRAPSEAPLLLAQSMTELTGPVLGEGPVAAADADLTLGQGGEAQGQRIVVHGQVRDADGRPVRNTLLEVWQANAAGRYLHKWDRHDAPLDPHFTGLGRTLTDADGHYRFVTVKPGAYPWGNHPNAWRPAHIHFSVFGQAFTQRLVTQMYFPDDPLFRYDPIYHSVPEFARDRLVAAFDLTATVELWALGFRFDIVLSGASATPMETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYX3</accession>
    <name>U5VYX3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_19480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362540.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19480"/>
    <dbReference type="EnsemblBacteria" id="AGZ42168">
        <property type="protein sequence ID" value="AGZ42168"/>
        <property type="gene ID" value="AFR_19480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3953"/>
    <dbReference type="eggNOG" id="COG0187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1325114_0_0_11"/>
    <dbReference type="OrthoDB" id="1280332at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="19877" checksum="670A396DB90805DE" modified="2014-01-22" version="1">MKEYTAADLTVLYFDESVRRRPAMYFGTDPHLATSVLTALVIASLHPGPKAAPTGPPDVTAEILDDLVLSITDNWPTGPDGSTAGYFGSVLTSCRWVHAAAAAVSTRTTIETWHAGRAWQQTLTGLRPTGPPRDLDAPPGTGTSLTYHLDPSRFAPSTLGALTRDVHAEGCCELPGEFRLHDRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ54</accession>
    <name>U5VZ54_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_13620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361069.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13620"/>
    <dbReference type="EnsemblBacteria" id="AGZ41010">
        <property type="protein sequence ID" value="AGZ41010"/>
        <property type="gene ID" value="AFR_13620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2766"/>
    <dbReference type="eggNOG" id="COG4334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133265_1_0_11"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016888">
        <property type="entry name" value="UCP028498"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10012">
        <property type="entry name" value="DUF2255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="123" mass="13393" checksum="D2F6580693EB7DED" modified="2014-01-22" version="1">MTDWIGQQAAQIAAPQEVQVATRRSDGSLRRATTIWIVRDGDRVFIRSTNGRTADWFRGATATGTGQIISGGRATDVVFTEAAEANLPAVDSAYRRKYSQYASIVDHLVQEGPRSATLQLQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZC1</accession>
    <name>U5VZC1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arsenical resistance operon repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20355"/>
    <dbReference type="EnsemblBacteria" id="AGZ42343">
        <property type="protein sequence ID" value="AGZ42343"/>
        <property type="gene ID" value="AFR_20355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4127"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13823" checksum="3A4AE23EB10DE612" modified="2014-01-22" version="1">MRAVHAFDVLGDPVRRRILELLAEGEQPSGAVTDVIRAEFGISQPAVSQHLKVLRDNGFATVRPDGARRLYTVDDTALREADAWLGRFRRFWTPHFAALATEVARGKRARRLDPPDDSEGDTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZC8</accession>
    <name>U5VZC8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Potassium-transporting ATPase KdpC subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP phosphohydrolase [potassium-transporting] C chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Potassium-binding and translocating subunit C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Potassium-translocating ATPase C chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">kdpC</name>
        <name evidence="13" type="ORF">AFR_13820</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit acts as a catalytic chaperone that increases the ATP-binding affinity of the ATP-hydrolyzing subunit KdpB by the formation of a transient KdpB/KdpC/ATP ternary complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The system is composed of three essential subunits: KdpA, KdpB and KdpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the KdpC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361109.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13820"/>
    <dbReference type="EnsemblBacteria" id="AGZ41050">
        <property type="protein sequence ID" value="AGZ41050"/>
        <property type="gene ID" value="AFR_13820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2806"/>
    <dbReference type="eggNOG" id="COG2156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077094_0_0_11"/>
    <dbReference type="OrthoDB" id="1912405at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00276">
        <property type="entry name" value="KdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003820">
        <property type="entry name" value="KdpC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30042">
        <property type="entry name" value="PTHR30042"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02669">
        <property type="entry name" value="KdpC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 11" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ41050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="29700" checksum="8E3FE6BB9678F77D" modified="2014-01-22" version="1">MRLPSWLSQHLAALRALLVFTVLLGLAYPLLLVGVGRLPGLNDRADGSLITVGDTTMGSKLIGQLFTDADGNPVPRYFQSRPSAAGDGYDPTATSASNLGPNSMVDTLSDDPEVASQSLLTQVCARSLAIGELEGVDGRRPYCTADGVGAVLGVFHRDGLTGPITRVVSVNQTGTPFTATYDGVTVEPAKNGEDYQALGAIMTPVRGDAPAKPAVPADAVTASASGLDPHISPAYAALQVPRIARERGLTEDAVRALVAGNTDDRALGFLGEPAVNVLELNLALDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZS7</accession>
    <name>U5VZS7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UvrABC system protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362865.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21105"/>
    <dbReference type="EnsemblBacteria" id="AGZ42493">
        <property type="protein sequence ID" value="AGZ42493"/>
        <property type="gene ID" value="AFR_21105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4278"/>
    <dbReference type="eggNOG" id="COG0178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001370_2_1_11"/>
    <dbReference type="OrthoDB" id="152379at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="2">
        <location>
            <begin position="449"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="487"/>
            <end position="494"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="754" mass="80958" checksum="178C97D55695EBA6" modified="2014-01-22" version="1">MPAEHVADSHGVIQVRGARENNLRDVSLDLPKRRLTVFTGVSGSGKSSLVFGTIAAESQRMINETYSAFLQTFMGSPSRPDVDALHNLSAAIVVDQERMGANVRSTLGTATDAYTMLRIVFSRIGVPSAGSALAYSFNAEGMCPGCEGIGKVSTIDIEGLIDRDKSILEGAITVPQFSVDGWYVKGYADSGYFDPAKKLRDFTEQELDDFLHRPATKMKLNGINTTYEGLVPKVQRMYLSKDKEAAQPHIKAFIDRVATFAPCPDCGGTRLRPEALASRIGGRNIAECSAMQVSDLAVFLADLDEPSISPVLLTLRHTLDSLVEIGLGYLSLDRESGTLSGGEAQRVKMVRHLGSALTDVTYVFDEPTVGLHPHDIQRMNTLLLRLRDKGNTVLVVEHKPETIAIADHVVDLGPGAGPAGGEICFEGTVAGLRASDSLTGRHLDHKVALRESPRPFTEVLPIKGASLHNLKDVSVDIPLGVLTVVTGVAGSGKSSLIYGSMPRRDDVVVVDQSAIRGSRRSNPATYTGLLDPIRTAFAKANKVKPALFSANSEGACPNCKGIGLVYVDLAMMAGVASVCEECEGKRFLPEVLTYKLRGRDISEVLGMSVTEARDFFTEKQARAVLDRLVDVGLGYVSLGQPLTTLSGGERQRLKLAIHMAEKAGTYILDEPTTGLHLADVDQLLALLDRLVDDGNTVIVIEHHQAVMAHADWIIDLGPGAGQDGGRIVFTGTPADLVATSQTLTAQHLREYVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZT8</accession>
    <name>U5VZT8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362776.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20660"/>
    <dbReference type="EnsemblBacteria" id="AGZ42404">
        <property type="protein sequence ID" value="AGZ42404"/>
        <property type="gene ID" value="AFR_20660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4189"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069129_7_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="30"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="24542" checksum="96954DC003FE3BAC" modified="2014-01-22" version="1">MIYDLVNPWDPARNPADAFYHRLVMGAESVLDVGCGTGAMLHHAREAGHRGTLAGIDPNSAFLERARRRDDIEWVFGTAAAMPWEQEFELAVMTGHAFQCLIGDGEIRASLAGVRAALHEGGRFVFETRHPQARAWETWTPSHAVEVAGVRVVHQVESVVGDVVTFTETAYATERHVLRFLDLPVLNTLVTQAGFHIDAQYGDWHGGPLTPASTEIITVAVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZW9</accession>
    <name>U5VZW9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362689.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20225"/>
    <dbReference type="EnsemblBacteria" id="AGZ42317">
        <property type="protein sequence ID" value="AGZ42317"/>
        <property type="gene ID" value="AFR_20225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4101"/>
    <dbReference type="eggNOG" id="ENOG5030Z5E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1958413_0_0_11"/>
    <dbReference type="OrthoDB" id="1690796at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="14101" checksum="3C76D8E236E608E9" modified="2014-01-22" version="1">MSLTVSPDLLAVAERGEVPDAEFVDCVRTSLPYAWDVVSKVMADLEAGGEFADHEIPPPSDVERGQLLRALASDSIRGGLERHFGAKLAFQNCHRVAAFRFDAVGGDRYQAFVSPRGQLLNQSPELRNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0N4</accession>
    <name>U5W0N4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_21360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362914.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21360"/>
    <dbReference type="EnsemblBacteria" id="AGZ42542">
        <property type="protein sequence ID" value="AGZ42542"/>
        <property type="gene ID" value="AFR_21360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4333"/>
    <dbReference type="eggNOG" id="COG2270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017518_3_0_11"/>
    <dbReference type="OrthoDB" id="1249292at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024671">
        <property type="entry name" value="Atg22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11700">
        <property type="entry name" value="ATG22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="262"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="456" mass="48640" checksum="49C30CCC80CD1D7B" modified="2014-01-22" version="1">MTATTAPVSTRRERTGWYFYDWANSAFSTTVITVFLGPFLTTVTELAAGCELGADECNGRVHPLGISVAAGSYFPYLVSLSVLLTVFVLPVMGAIADRAERKKPLLATAAFIGAGATIAMAFVTGERYLLGGLLFVIANIAFGASVVVYNSFLPRLAGPDDRDKVSSRGWALGYLGGGVLLLINLAVVTMLSVDGDDQRTLDLARWSIVSAGVWWGAFTLLPLRWLKEHPAAEVVAGRGGNVLTDGFRQLGHTIRSLQAYPLSLFFLVAFLVYNDGIQTVISLASQFGTEELHLEQSALIITILIVQFLAFGGALLLGGLAAKIGARKTVLIALAGWLGVVLAAFWLPEGEPVPFMLLGVGIGLVMGGSQALSRSLFSQLIPEGREGEYYGFYEISDKGTSWLGPLAFGLVFQLTNSYRIGIVSLVVFFVVGGILLALVPMRRAIVAAGNTPPRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0P6</accession>
    <name>U5W0P6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21435"/>
    <dbReference type="EnsemblBacteria" id="AGZ42557">
        <property type="protein sequence ID" value="AGZ42557"/>
        <property type="gene ID" value="AFR_21435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4348"/>
    <dbReference type="eggNOG" id="COG3903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004665_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="2">
        <location>
            <begin position="105"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="773" mass="82118" checksum="9B50F763B48EE1AD" modified="2014-01-22" version="1">MVPLLIVTSGLFSVLLAVAVNVATGGVLPGGLQPWAWLAWPLVGLLAMVGVGLALWQQRLSAAASPEAAPPPVALPAELPAAPVVLGRDGELAALESAVGGGAQLLVIAASAGTGKSALGLRFAQVMRDSYPGGQLYAALQGASADPVLPEDVLVRFLRALGRPDDELRGDVVELSARFRSVVADRRVLVLLDDARDAAQVRHLLPGGEHSLAVVTSRRLLSDLLGASVFTLGGLDPGSALELLAVTAGGGRVAADPDGARRVVQLCGGLPLAVRIAGGRLKARAQWTPTALADRLVDEERRLDELRLADRAVRSSFQAVYDELAPGEQVVFRLAGSYPGASVGLGAAAARCDLDEQAVADALERLTDAFLVESPAPDRYRLHDLLRLFAQELAGGEAAECLERHLRWLSTRARPGVWLAEEREDIVAVLHAAVAAGLEAPARRLIGAVHPLVTGGTEHAYRLRLWQAGVAAAGEDEAFRVRALRWVSHSYGMAGQVALELQTAEQALDGAERLGSDVREIALAAWRVGDALRAQDRFTESEAALLRALDLLAGLGAVDDEIEVRLALGTLYNTFWKPELSGPLLARALELLPDGPQPVRGWAVLGLGLAHKLGGDQAKARDLYAEAFVIAQRSGDDFLLGYCLQERGWLAAQQRAFDAARADFTEMLAIFERMRSGTGVGGAHAALGEIADMQGQWEAALAHYDAGAAEFERLGMWVRLGEVLLHRSTVLKMLGRELEAEAARERGEALVGDAPLHRGPGLVRRLNEPTEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Z1</accession>
    <name>U5W0Z1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16710"/>
    <dbReference type="EnsemblBacteria" id="AGZ41620">
        <property type="protein sequence ID" value="AGZ41620"/>
        <property type="gene ID" value="AFR_16710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3388"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012705_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKD" evidence="2">
        <location>
            <begin position="359"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="1">
        <location>
            <begin position="625"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="1">
        <location>
            <begin position="770"/>
            <end position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="922" mass="97494" checksum="19F2ACCD3039C262" modified="2014-01-22" version="1">MLYTYDAPPGQTAPVWNDNCTSVGGTNGGRCIVTGRLSRLQAAGNVMTGPEQVLVQDWCQQFSSHSIGDLHFGGDGMLYASAGDGASFNVADYGQLGTPINPCADPPGGTMTPPAAQGGALRSQDVRTTGDPAGLDGTILRLDPLTGAAAAGNPAIGSADLNTRRIVASGLRNPYRFAMRPGTNEVWIGDVGWNTWEEINRLSNPTAGVANFGWPCFEGTGRQGGYDGANLDMCENLYSAGGQTAPFYTYQHTEKVVSGETCPTGGSSTSGMAFYPTSGGDYPAAYQGAVFFSDYTRDCIWAMRPASPGGVPVAANRITFAASAANPVDLAIGPGGDLYYVDAGGTIRRVRYFAGNQPPNAVLVAAPTTGPGPLTVSFDATASDDPDPADEGLLRYEWDFTDDGTVDARTATATFTYPPGGPYTARLRVIDTLDVADTQTVAIQAGNTPPEAVIDTPEAGTTFAVGQNLTFTGHATDPELGDLPASALRWRLLQHHCFTLDSCHIHTAQEWNGVAGASFPAPDHEYPSYLELVLTATDSGGLSSTTTRRIDPRTVDLTFTSNPSGMMVAVGSTAQATPFTRTVIQGSENSVSGVTPQSAAGTPQIYAAWSDGGAQTHVIVAPSTPKTYTATFVRQRVTQSQMRVRSVDSQETAGQDGRGLNVLDGNPATMWHTQWQAADPPHPHQIQLDLGATYSVTNLYYLPRQNSANGRIARFEISVSTDGTTWGTPVATGTWPSTTAEQTVTFAGKTGRYVRLRSLSEVGGRAWASAAELNVGVAPRVPRVTMKVRSVDSQETAGEDGRGVNALDGNNATYWHTQWQAASPPHPHEIQLDLGRAGSVSCVYYRPRQNSPRGRIATFEVHTSTDGTNWGTAAATGTWLDTVNEQNACFSARTARYLRLRALSEVAGNPWTSAAEVGVIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W121</accession>
    <name>U5W121_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363350.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22870"/>
    <dbReference type="EnsemblBacteria" id="AGZ42844">
        <property type="protein sequence ID" value="AGZ42844"/>
        <property type="gene ID" value="AFR_22870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4635"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_9_0_11"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="117" mass="12356" checksum="53DCF1D7165EF909" modified="2014-01-22" version="1">MSKGEWELTGDRLVEVLATLASPHRLRVLAALTGGRTYVSQLARDLGISRALLQVHLKKLEKAGLVTAQLELSEDGKALKFYEATAFSLHLTPDVVAAAVTTLSTAGDGDAVPKGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1B2</accession>
    <name>U5W1B2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17360"/>
    <dbReference type="EnsemblBacteria" id="AGZ41750">
        <property type="protein sequence ID" value="AGZ41750"/>
        <property type="gene ID" value="AFR_17360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3520"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="151"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="308" mass="32056" checksum="3358D63E89987DDD" modified="2014-01-22" version="1">MGLIGAGGVAQRHARVLSGLPDVQVVGVTDVVPEAAEKLAAEHGTRALPDVAALLESGLDAAYVCVPPFAHGPAERAVIAAGLPLFVEKPIALNLDDAQEINGLIAERDLLTSVGHHWRYLAIVEQARELLGDRPVRLVSGAWLDKVPPVGWWPHRDRSGGPVVEQAAHVLDLARHLAGEVVEVSAIGNPAPPVDGADVDSATAATLRFAGGAVGTLAATCVLGWKERAGLEVYADGLALAITETSLVVRDADGTRTYESDPDSARVAVDRAFIEAVRGEGSDIRVPYAEALKTHALAVAVAESVAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W207</accession>
    <name>U5W207_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363853.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24985"/>
    <dbReference type="EnsemblBacteria" id="AGZ43263">
        <property type="protein sequence ID" value="AGZ43263"/>
        <property type="gene ID" value="AFR_24985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5061"/>
    <dbReference type="eggNOG" id="ENOG50341M7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149990_0_0_11"/>
    <dbReference type="OrthoDB" id="1903502at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="116" mass="13672" checksum="A19B87D3E67B0AED" modified="2014-01-22" version="1">MLDFKDLAPAIHRQRMVVEGYPTFVITDEHIKDYLRKLSDVTEMITIIDPVTHVSDLYGWAGWIHWETSGAHFYSWERPISFFSVDIYTCKAFDPQLVLDFTEAYFRTTEIVAKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W262</accession>
    <name>U5W262_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362404.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18775"/>
    <dbReference type="EnsemblBacteria" id="AGZ42031">
        <property type="protein sequence ID" value="AGZ42031"/>
        <property type="gene ID" value="AFR_18775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3813"/>
    <dbReference type="HOGENOM" id="CLU_627949_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="436" mass="46854" checksum="2BCB11FE788122FE" modified="2014-01-22" version="1">MERNELVLYADGANDHLDLALRPGRPLGWATLDTGGTLAGTLGELGAERLGRRYPVLFDDGDRQELTESLARRAHGSPYFDPALILLDAFAPAGLPVPGFVALVSSAQLSVLPPCRSPEGVVLGNARLIRTVRPADPAPGTLEYQGAEYLTEHAPGPRDGELLLRTTPSSPFADLTGNRHSYFCVLNPVHAPHFTTDYLYVRNPFLAADHPGLETAIPVRVVFDDRVPAARVVLDSTTLFAIGLPQGEPAVLGPCHGRAGRVRNTLFGHRHAVARVGRTAVIDIEKPVTRLPEEMLDLLGISQGDRVIVEAAAHRAGHIEIARTTLRALPWREQSPLLLARSRTGVPDHQLLVGDEDFPMISLDLNARTTLGIVPGSAVYVRPALSSLLGREFTSVSFVLIAAIFSAAALQSLALAVVSASIYLVLTVFIVGARLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2H7</accession>
    <name>U5W2H7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">C4-dicarboxylate transporter/malic acid transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_25870</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25870"/>
    <dbReference type="EnsemblBacteria" id="AGZ43438">
        <property type="protein sequence ID" value="AGZ43438"/>
        <property type="gene ID" value="AFR_25870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5244"/>
    <dbReference type="eggNOG" id="COG1275">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030057_6_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="360" mass="37639" checksum="68F0952A9DE6EFA4" modified="2014-01-22" version="1">MEIMTALLERPARRTTPVTLFGPNWFASVMGTGIVATAAASLPVHVPGLRVLATVVWALAAVLLVVLLVATVRHRTSYADDPVLFHFWGAPPMALMTVGAGTLLLGRDWLGEPLAVVVDLVLWTAGTGFGLVVAVLVPYRMMTRTGDRPDAAFGGWLMPVVSPMVSAATGALLVPHVPARTELLLACYAMVGISLFAALVVISQIWGRLVRHGVGPARLVPTLWIVLGPLGQSVTAVHLLGRASDGVLSEPYAQGAQLAALLYGVPVLGFAAFWAALAGAITVRTARAGLPFSLTWWSFTFPVGTVVTGVSGLAVRTGSALLTAVAVLLYAALVAAWTVVATRTLRGVRRGDLLKVAPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2W5</accession>
    <name>U5W2W5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43474">
        <property type="protein sequence ID" value="AGZ43474"/>
        <property type="gene ID" value="AFR_26050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26050"/>
    <dbReference type="HOGENOM" id="CLU_2679340_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="74" mass="7704" checksum="04DDE48FAA4131B7" modified="2014-01-22" version="1">MQQPLELVAANGHLMPVPQLTEPRAASGELVDEPDGVRIRRVPTGDAGAQVGDQGVCLRVMVLGRKLGAYAGIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3E3</accession>
    <name>U5W3E3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362887.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21215"/>
    <dbReference type="EnsemblBacteria" id="AGZ42515">
        <property type="protein sequence ID" value="AGZ42515"/>
        <property type="gene ID" value="AFR_21215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4302"/>
    <dbReference type="eggNOG" id="ENOG50320SQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136162_0_0_11"/>
    <dbReference type="OrthoDB" id="2086605at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25271" checksum="FE31F84ED81A0AF5" modified="2014-01-22" version="1">MTDELRDLLRAELDAERPPPLGDLVGTALADGRRIRRTRRLAAIGGGTAVAGVMAVTIALGDPFGSAGAPAAVIGGPATALPAVSPPPKRSGPQATATPEAMLVLLQKLVPPGRTTGFAKTGQRELHVQMYLDRGKGPGMIRLSVSGTSRAAPAAGRPAVIVDGLPDNCIQSKIVRAEWPGGLTVQADLSTCLAWDGRRNKPSHLALTDDEAVAVVTDPRWGPTMDADLVKAGAKEFPRVAAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W456</accession>
    <name>U5W456_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560335.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28685"/>
    <dbReference type="EnsemblBacteria" id="AGZ43998">
        <property type="protein sequence ID" value="AGZ43998"/>
        <property type="gene ID" value="AFR_28685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5815"/>
    <dbReference type="eggNOG" id="ENOG5031FJR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151343_1_0_11"/>
    <dbReference type="OrthoDB" id="2104395at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045428">
        <property type="entry name" value="EACC1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19953">
        <property type="entry name" value="EACC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="12942" checksum="230D016F44DFE925" modified="2014-01-22" version="1">MNVFLTASGDDDQVRSIDEVQDWLDHEPDLQGRISRTPAKPRPGELGAGWDTLTVAVGAGGALSVLGASLKVFFAQPRRTDLKLTVRAADGRSVVLDAKRVKEADVVTVLRETLGGATSDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W489</accession>
    <name>U5W489_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560365.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28835"/>
    <dbReference type="EnsemblBacteria" id="AGZ44028">
        <property type="protein sequence ID" value="AGZ44028"/>
        <property type="gene ID" value="AFR_28835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5845"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987856_0_0_11"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13520" checksum="10EE19A5C93FBCCA" modified="2014-01-22" version="1">MAWSTRELAELTGTTVNTIRHYHRLGLLDEPKRSSNGYKQYGDEELDRLVRIRRLVELGVPLARIGEAGTDGTLPPETLRQVDADLAANIERLARARSDIAAILRDGAPAAAVASRLCAAGSQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4D0</accession>
    <name>U5W4D0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DeoR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_22555</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363202.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22555"/>
    <dbReference type="EnsemblBacteria" id="AGZ42781">
        <property type="protein sequence ID" value="AGZ42781"/>
        <property type="gene ID" value="AFR_22555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4572"/>
    <dbReference type="eggNOG" id="COG1349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060699_0_1_11"/>
    <dbReference type="OrthoDB" id="1629121at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014036">
        <property type="entry name" value="DeoR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018356">
        <property type="entry name" value="Tscrpt_reg_HTH_DeoR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00455">
        <property type="entry name" value="DeoRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08220">
        <property type="entry name" value="HTH_DeoR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00037">
        <property type="entry name" value="HTHLACR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00420">
        <property type="entry name" value="HTH_DEOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00894">
        <property type="entry name" value="HTH_DEOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="27127" checksum="2DF062303F1A244E" modified="2014-01-22" version="1">MPQSSLRRADRVSAILERVASHGSVDAGHLADEFEVSPATIRRDLQVLEDQRLLSRTHGGAVAVDVAYELPVRYRVGQHREEKALVAQRVAALLPKGPLTLGLTGGTTTHLLARLLAERVDLTVVTNALNIAAELALRPRLKLIMTGGVSRTQSYELVGPIADQALAGLNMAVAVVGVDGISARGGLTTHDEIEANTNATMIRRADRVIVVADGSKVGRVCLASICAITGVATLVTDSSADPAGVEAIRRTGTEVIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4G3</accession>
    <name>U5W4G3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23050"/>
    <dbReference type="EnsemblBacteria" id="AGZ42880">
        <property type="protein sequence ID" value="AGZ42880"/>
        <property type="gene ID" value="AFR_23050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4671"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="825"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="890" mass="95585" checksum="C6726220B4EC124E" modified="2014-01-22" version="1">MVAAARSGESRVLVVHGPPGVGKTALLDHARQAATGLRVLRVFGVESEMELAFAALHQVCAPLLELLPRLRAPQRTALETVLGLRAGAPPDRFLVGLAVLSLLSEASEQGPLLCVVDDAQWMDRSSAQTLSFVARRLLAESVALLFGTRDRDPDLLGLPELEVTGLRDADAHALLDAVTRGTLERHIRDRIVAETRGNPLALVELPHGLSVTELAGGLGLLRAGALPGRIEQSFLGRIRELPDETRTLLLVAAAEPVGDPAPVWRAAGRLGVTPATALAKGTDGLLSIEERVTFRHPLVRSAVYHSAEPGERRAVHLALAEVTDGTTDPDRRAWHLASAAAAPDEAVAAELERSAGRAQARGGLAGAAAFLQRAVQLTTDPARRADRALAAAGATLQAGDIEAAGRLTAIAVRDAPDEFTRARAALIRGHIAFSAGRHDEAPRGLLAAAQRLEPFDAKLARETYMLAWGAAFMGGGQEDLLAISRAVAALRRRGGPPLPIDLVLDGFSSLVLGSRTDAVRVLQQATVALADLPVEDILLWGWVANTVPVSVWDDVAMQTITDRWVDVIRAAGALGHLPLYLTGAGMSRSFTGDLATAGDLASEADTVAAALALPISPYLQLRLTALRGREPEASALITETIARADAAGEVLGVTIAQWSAAVLDNGLSRFDQAMSAAQVCTRIAELWISVWALPELVEAAARLGELEIARAALDRLTESAGPCDTDWAQGTLARCRALLSESSAAEGHFRTAVERLGRTELRPELARAHLLYGEWLRRDRRRIEAREQLRTAYEMFVLIGMEAFAERARRELLATGETVRKRRVEAAAPDQLTPQERQIAVLVREGFSNPEVATRLFISPRTVEWHLRKIFTKVGVTSRRQLREALPRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4I4</accession>
    <name>U5W4I4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative non ribosomal peptide synthetase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_29235</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29235"/>
    <dbReference type="EnsemblBacteria" id="AGZ44108">
        <property type="protein sequence ID" value="AGZ44108"/>
        <property type="gene ID" value="AFR_29235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5924"/>
    <dbReference type="eggNOG" id="COG1020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_0_9_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensatn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="514"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="1565"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="1646"/>
            <end position="1721"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="549"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="1600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1988" mass="215116" checksum="7641C85305B47AD7" modified="2014-01-22" version="1">MLDSSPGNPPTLPELFAAQVEHGASEVAVVGDDASLSFAELDERSNRLARLLIDQGVGPEVVVALRMSRSVDFVVAALAVVKAGGAYVPVDVAYPAARAEFMMADARARCVVTTTSAASPGSSAIPQVVLDDPKVVDELAGLDGHAVRDADRHEELLPDHAAYLIFTSGSTGVPKGVVVTHRSAQNLVRAQAQILGLGPGRRRLQFASVSFDASVSEVWTTLLSGAALVVADASRLVPGPALVDLVRSRGVTHVTLPPSLLSAVEDAGGLPDAVTLVVAGEACPPAVAARWCRDRRMLNAYGPTEATVAATVSDPLTGDGTPPIGRPLPNVRVYVLDELLREQPVGVPGELYLAGAGLARGYAGRTALTAQRFVADPFGTPGERMYRTGDVVRRRADGQLEYQGRADDQVKIRGVRVEPGEIESALMGLAGVDQAVVVAHGDGADRRLVAYVVADADGPDAAGLRRNLAAVVPDHLVPSVFTMLDSMPVTVNGKVDRKALPDPAGDAGVRDFVPPVGAVEQTLARLWEDVLGVPRVGRTDEFYRLGGHSLLATQLVNRVRTAMNVELPVRDLFETSTLADLARRVSVLKSGTRPVLRRQVRAATVPLSYPQQQMWLTAQLRGGDGAYHIPVVVRLSDGLDEAALRAAITDIVRRHEPLRTRFPVLEGQPRQQVVPMADLGELLVREPVAAGDREDTVRRVVREGFDLTTQIPLRARLLEFGPDDFVLVLVLHHITADGASMEPLMRDLQEAYERRRAGRPPQWTELPVQYADFAVWQRELVRLARPQIAYWRDLLADAPHELALPVDRGRPAEPTYRGRSVPISVPAEVHEQIAATARRHGATVFMVLQSAVAALLSRLGAGTDVPLGWPVSGRVDEALEDLVGFFVNTLVLRADLTGDPSFDELIGRVREQDLLAFGHQDVPFESVVEELNPPRVPGRHPLFQVMVASQVGRSARFGLAGVRSERMGVGSGSAKFDLSVQFVEFLDESGRPSGLGGEVMYACDLFDHDTVERMVGMLGRLLAEAVAQPGLPIGRLPVMSGEERLAVEAASTGDVRDWAARPWPHQLVEQHARRNPDAIAVRFEGVDLPYGELNRRANQLAHHLIELGAGRETLVGISMERGFDLVVGVLAIWKTGGAFVPLDPDLPAARLTSMIGDAAVGVVLTHRATQDRLAAHDDLLLCVDGPDTDLAARPSHDPGVPVDGEDAVYAIYTSGSTGTPKGVVNVRAAIRNRLTWMNERFGAGTDGRVLQKSPFTFDTSVWELFWALTNGATLVMARPDGHRDPRYLVDLIQREQVTLVDFVPSMLEMFLREPDAARCTSLRHVIAGGETLTRSLRSRFAEALPQTEIYNLYGPTEAAIGVTSWLCRDDDDSRSAPIGVPIANARVYVLGPDLGHVPPGMPGEIYIAARPLARGYLNRADLTAQRFVADPFVAGERMYRTGDRARRRPDGAIEFLGRDDEQVKIRGVRVELGEVEAALLGLHGVEQAVVTVHGDGVDKRLVAYVVGFDPDPVRLRRQMPAVVPAHLVPSAFVAMDAFPVTVNGKVDRRQLPDPGVVDTDRAYEAPAGPIEESLAGLWQELLSVARVGRDDNFFALGGHSLLAMQMVNEIRSLLRVELPFRTVFESSTLADLAARIGDLPLLPDADSQASPEDRMRVLVAQALGVDEVAAEDNLFDLGGGRETVEALTRAIEAEFGMTVNPLVVSAVPTAKDLAGWLAAEPEEATLDVVVPLRPGTTPESLFCIHPLGGLSWLYYPLVNAIPSAVGVYGVQARGLGPGEELPTTMTAMAADYVQQIRLVQPEGPYHLLGLSTGGEIAHEMGVQLQRGGEEVALIAMLDARPTPQRPATQVERLNAVAHHFDLNVPAEERPHLTGDILYEQLRQRQGPYSFLMRQKGRATVDFYENMIKVADGHKFSVFDGDVLFIEAIAARPEEHYFAPMWHPYVSGAVDVMALDYKHRQLARAEVLQLIGARVAEHLRLTPTEGPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4J9</accession>
    <name>U5W4J9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44128">
        <property type="protein sequence ID" value="AGZ44128"/>
        <property type="gene ID" value="AFR_29335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5944"/>
    <dbReference type="HOGENOM" id="CLU_1264674_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="24370" checksum="F16632DFADC001B7" modified="2014-01-22" version="1">MLRRFVGLLEADLAAAGFVRRGPVFRYFDPEGNGIALNIQRTTALQGEVAFFINVGVLLAPHLRYYFGEDDARRDAMPHHSVWNHRLVATDDTAELPDHTFTLSTEADVEYAATIVRVWLAKNLPGMKTWLGDTDAMLAAAEADRERAARARTEQLASGRWKSGAWPEGRWSTRLIAAYRHAERGDVDAVMDFPHSDPDSVGADIVALARRRAAERKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4P3</accession>
    <name>U5W4P3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_29115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560421.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29115"/>
    <dbReference type="EnsemblBacteria" id="AGZ44084">
        <property type="protein sequence ID" value="AGZ44084"/>
        <property type="gene ID" value="AFR_29115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5898"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="89"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="310" mass="33291" checksum="A9CE510B0D6F7F68" modified="2014-01-22" version="1">MTVIDNAAGAAAVATGRSPGRPARRPGSAMSYGFVSIYAFLALVFGILPAVYALYLAFTDAEGGFAGLANFTKVIGDFRFWPAATHVALYLVIWLVALVVLVTLLAVIVHAIRVRWLSRTLRFVFYLPGALAGASSVLLWLFVLDPTASPVSGLLRLLGFDSFVQVIVPGNLPVVFAIIAFWTGAGGWIVIMYGALNNISAEVIEAARVDGANAVQIAWRIQLPMLRKWISYMGIMSLAAGTQLFVEPQLLSQASNAVVPNDYSLNQLAYQYAFQQNDFNGAAAISLLLLVIALALSAVFVTRGGLFERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Z0</accession>
    <name>U5W4Z0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560536.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29690"/>
    <dbReference type="EnsemblBacteria" id="AGZ44199">
        <property type="protein sequence ID" value="AGZ44199"/>
        <property type="gene ID" value="AFR_29690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6018"/>
    <dbReference type="HOGENOM" id="CLU_738955_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="374" mass="40509" checksum="9D201A559D3DC721" modified="2014-01-22" version="1">MREASIETLRDELAVAADTAVQRPVLIQSPGRQTIRAALAPWVREAARQRQVTIIASANESLMRWVDWLSDYEDVSLHKLNAATALESLDEHLDSARVTLATFAALRRQAVKEGLAASHRDVVIVDTLPLPGGQAYQLVAELTAGARYAVAISDSQAPESLPGAETIVVPAQKLYPGHRHDVVVKPINVLQSTAERQILAEAAPLLAGDADVSRSAAHAKLLVAAAQITDGDFGRTDAIWGLIDRIEALTADERLSAFTDLVAQYADRGPVVVLTGPLRAEAEYVRSYLESAGIAVVAQGRAELRLGRVTEEIPDHVRPVIISSWQSIEPDEVWLRDSTVIFWSQPAIRQAPLISLLSDIGAVREIIHLVDTPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5G5</accession>
    <name>U5W5G5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peroxiredoxin Q</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24450"/>
    <dbReference type="EnsemblBacteria" id="AGZ43156">
        <property type="protein sequence ID" value="AGZ43156"/>
        <property type="gene ID" value="AFR_24450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24450"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_5_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AhpC-TSA" evidence="1">
        <location>
            <begin position="38"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="203" mass="21859" checksum="ECB6345A109E4237" modified="2014-01-22" version="1">MFFMTTAPLHLADADHAAARRQFERDAVWSRMIAPGGRVPALPLIEVDLGPIHLDRLLQTGPLVLAFFRYEGSAAGDAMLVDYQRTLLPACTDLGAHLVAVSPQRFDLLAPVKHRHELGFLVASDPRHVLIDAFRIGYGSPAASDTLGTRRSVLPYPAVVVADRTGTVRYADVRAESAPHSGAAPIIAALRRSAGSSPVPARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5U0</accession>
    <name>U5W5U0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">DNA-directed RNA polymerase subunit omega</fullName>
            <shortName evidence="11">RNAP omega subunit</shortName>
            <ecNumber evidence="2 11">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RNA polymerase omega subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 11">Transcriptase subunit omega</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">rpoZ</name>
        <name evidence="12" type="ORF">AFR_30585</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9 11">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the RNA polymerase subunit omega family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_015623990.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30585"/>
    <dbReference type="EnsemblBacteria" id="AGZ44377">
        <property type="protein sequence ID" value="AGZ44377"/>
        <property type="gene ID" value="AFR_30585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6192"/>
    <dbReference type="eggNOG" id="COG1758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125406_1_1_11"/>
    <dbReference type="OrthoDB" id="1864134at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00366">
        <property type="entry name" value="RNApol_bact_RpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003716">
        <property type="entry name" value="DNA-dir_RNA_pol_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34476">
        <property type="entry name" value="PTHR34476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00690">
        <property type="entry name" value="rpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 11 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ44377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="87" mass="9407" checksum="B5D00F8C86136C68" modified="2014-01-22" version="1">MGTIANPEGITNPPIDELLDKTSSKYSLVIFAAKRARQVNAYYSQLGEGLLEYVGPLVETTPQEKPLSIAMREINAGLLTAEATDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5V9</accession>
    <name>U5W5V9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_25605</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364035.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25605"/>
    <dbReference type="EnsemblBacteria" id="AGZ43385">
        <property type="protein sequence ID" value="AGZ43385"/>
        <property type="gene ID" value="AFR_25605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5190"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_11"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="21"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="300"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="394" mass="41055" checksum="BC8EF7D96EFB61A2" modified="2014-01-22" version="1">MRLAEAWRVALDALRANRLRSVLTMLGVVIGVAAVVALVAIGTGTKQQIEQQVEGLGSNLLIVVPGRLEAGSAPSVSTLSLDDVDTVSRVVGDRSRVAVTVASGETVRAGSRTAFASMQGVLETTPSVFVRRLDRGSYLTRSDVSTARRVAVLGAGVATQLFGDRDPIGRQVTIGGVRFRVIGTFEPLGQSLGVDRDSEVHIPVTAAQRLLGTDRIDALAIRAPDRERIGTLGADIVAALTRSHPDSDFSAVTQEQILGVLGDILGVLTGVLAAIAGISLLVGGVGVSNIMLVSVRERTREIGLRKAVGARPRDIGLQFLLEAVLLTTIGGVIGMALGITAALLVDRLSPVPAAVTWWSLALAFGVSALVGIIFGVVPAQRAGRLDPVVALRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6B3</accession>
    <name>U5W6B3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561002.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32055"/>
    <dbReference type="EnsemblBacteria" id="AGZ44669">
        <property type="protein sequence ID" value="AGZ44669"/>
        <property type="gene ID" value="AFR_32055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6490"/>
    <dbReference type="eggNOG" id="ENOG50335CN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_958556_0_0_11"/>
    <dbReference type="OrthoDB" id="1695614at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046037">
        <property type="entry name" value="DUF5995"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19458">
        <property type="entry name" value="DUF5995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="257" mass="28816" checksum="B41434E8FB3830F9" modified="2014-01-22" version="1">MTADSVWGPVQQDMADVLAGHPDDVPAVVDQLTKLQDVLLRVPPLLGSNPLADFNKLYLTITTSVLERLYAGRFADPAFLSRLDVEFAARYFDALRQWSDMSAGCPRAWSVLFHRIPGPDARPLPSAAAGVNAHINFDLPFALVTTFDHLGSDPCDGSDQHHDYLQINAIFAESIPGLRRGFLEKWQLIIDMMNGDLDDWWQGELVEYTRNVAWRNSQKLWAVRHDLKALGRERERLDCTAEAFGKLLMSPMGAVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6C4</accession>
    <name>U5W6C4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561092.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32475"/>
    <dbReference type="EnsemblBacteria" id="AGZ44753">
        <property type="protein sequence ID" value="AGZ44753"/>
        <property type="gene ID" value="AFR_32475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6572"/>
    <dbReference type="eggNOG" id="COG4753">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139202_1_0_11"/>
    <dbReference type="OrthoDB" id="1963330at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13289" checksum="76756E204A7ACC73" modified="2014-01-22" version="1">MANIETSLKEAMTIEGAIGVALVDYTSGLTLGVLGGGPNMDMTIAAAGNTDVVRAKVRTLEMLGLSDSIEDMLITLDSQYHLIRLLEKSRGGDALFLYLALSKSRANLGLARHQLRKIEADLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6K2</accession>
    <name>U5W6K2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32925"/>
    <dbReference type="EnsemblBacteria" id="AGZ44843">
        <property type="protein sequence ID" value="AGZ44843"/>
        <property type="gene ID" value="AFR_32925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6663"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="6"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="30138" checksum="3C7D3E944778A491" modified="2014-01-22" version="1">MFDRYGPPEVVHIVELPDPVPGPGQVRVRVRAAGVQPFDVGVRRGWMRPGPEHFPHGIGQEYSGVVDLLGEGVSGLSPGDPVLGSTMMNGQATHVVVDAGTVVPKPAEVDFPTAAALVAAAQTASGALLELGLTAGDVLLVHAAAGSVGTVAVQLARLAGATVIGTASPANHDYLRQLGAIPVAYGEGLVAAVRAAAPGPVTVALDAAGRGAIAASVELGVPRDRIGTIVDDKAAADFGTRVVRAGRSPARLAEVVGLAARRVVTMPVRAYPLSEAAEAHAAVENGHGRGKVVLIVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6K6</accession>
    <name>U5W6K6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA ATPase forming ring-shaped complexes</fullName>
            <shortName evidence="2">ARC</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">arc</name>
        <name evidence="5" type="ORF">AFR_26420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer. Assembles into a hexameric ring structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26420"/>
    <dbReference type="EnsemblBacteria" id="AGZ43546">
        <property type="protein sequence ID" value="AGZ43546"/>
        <property type="gene ID" value="AFR_26420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5355"/>
    <dbReference type="eggNOG" id="COG1222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036054_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02112">
        <property type="entry name" value="ARC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041626">
        <property type="entry name" value="Prot_ATP_ID_OB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022482">
        <property type="entry name" value="Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17758">
        <property type="entry name" value="Prot_ATP_OB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03689">
        <property type="entry name" value="pup_AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="232"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="8"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="243"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="555" mass="61646" checksum="617112719F3164EC" modified="2014-01-22" version="1">MTESPRHVRQIEERLAATQTQLARLTENNDRLVATLKEARSQIVTLKEEIDRLAQPPSGYGVFLAAHEDGTVDVFTGGRKLRVAVSPSLEVEELKRGQEVLLNDALNVVDAFGYERVGEVVLLKEVLENPAGGPGDRALVISHADEERIVHLADSLQVSKLRAGDSLMIEPRSAYAYERIPKSEVEELVLEEVPDVDYTDIGGLHSQIEQIRDAVELPFLHAELFREHQLRPPKGILLYGPPGCGKTLIAKAVANSLAKKIAERRGEEKHTSYFLNIKGPELLNKYVGETERHIRLVFQRAREKAGEGTPVIVFFDEMDSIFRTRGSGVSSDVENTIVPQLLSEIDGVEGLENVIVIGASNREDMIDPAILRPGRLDVKIKIERPDAEAAKDIFAKYILSGLPLSTDDLAEHGNDRDVTVAAMIEAVVLRMYTETEENRFLEVTYANGDKEVLYFKDFNSGAMIQNIVDRGKKMAIKEFLTSGKKGLRLQHLLDSCVDEFRENEDLPNTTNPDDWARISGKKGERIVYIRTLVSGGKGAEAGRSIETASNTGQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6P3</accession>
    <name>U5W6P3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26620"/>
    <dbReference type="EnsemblBacteria" id="AGZ43586">
        <property type="protein sequence ID" value="AGZ43586"/>
        <property type="gene ID" value="AFR_26620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5395"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_7_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="26"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="18490" checksum="3CEAAF60828F71FD" modified="2014-01-22" version="1">MIADTPVTATLTCMGEREVVPGAAAGGPISHTIFRLARIHRLIAGRLLREVGLHASQELLLMRLWDGGPQRQTDLGAELGTDSAATTRIVQRLEESGYVRRVPDPADRRATLVENTAQGLALREHVERIWEHLEELTVGEMTPEQQQTALDCLLRLECNLHTGVTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6W9</accession>
    <name>U5W6W9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA polymerase sigma factor rpoD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_27050</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27050"/>
    <dbReference type="EnsemblBacteria" id="AGZ43671">
        <property type="protein sequence ID" value="AGZ43671"/>
        <property type="gene ID" value="AFR_27050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5482"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014322">
        <property type="entry name" value="RNA_pol_sigma-B/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02980">
        <property type="entry name" value="SigBFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="7">
        <location>
            <begin position="34"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r3" evidence="6">
        <location>
            <begin position="113"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="8">
        <location>
            <begin position="198"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="27607" checksum="C2DFEBEFB6514BB6" modified="2014-01-22" version="1">MSEASAPGSPAELLDRMAATPPGARRRAARDRAIEAWLPLARHLAHRYSGRGEPTDDLIQTATVGLIKAVDKFDPERGVDFAGYAIPTIIGEIKRHFRDRTWSVRVPRRLQELRLSITEANSTLTHTLGRSPTVADIAVHLGVTEEDVLEGLEGARAYNATSLSTPISADGTTELGDTLGGEDHEFELAETRVALGPALALLDEREQKILTLRFYGNLTQSQIADQVGISQMHVSRLLTKALTKLRTELANSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W745</accession>
    <name>U5W745_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33800"/>
    <dbReference type="EnsemblBacteria" id="AGZ45018">
        <property type="protein sequence ID" value="AGZ45018"/>
        <property type="gene ID" value="AFR_33800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6842"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_586131_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665769" description="F5/8 type C domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="22"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="423" mass="44882" checksum="F4CD8B7DE47F14E3" modified="2014-01-22" version="1">MRRRSLTIAAAVAAVLTTYVVGTQTGASAADGLLSQNRPATASSTESGAFPASNAVDGNAGTRWSSAFSDPQWIQVDLGGTATLSSVILDWESAYSRTFNLQTSANGSSWTTIKANVTGTGGRQTVAVSGSGRYVRLQSTARATQWGVSLAEFEVYGSGGGTAPPPTIPPGAVRVSEFLADCPYSHRLPDDPIVFPNMPGASHMHSFFGSTVTNAYSNVNDLLNANSNCNPSIDKSSYWIPTFYNNNVPVEPTTGIFYYLGEGVSDALIAQTQPLPLGLRVVAGNAKATGPSDNTISRWSCLHAGEVGSSHDFVTCPAGTMLESYLDFPHCWDGVNLDSPDHKSHMAYPVNNACPASHPVVVPKLRQVMRYPVNGSTAGFRLASGAGYTMHGDFFNAWPVEEMARRVNDCIRIIVKCGTNGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W763</accession>
    <name>U5W763_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_33605</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33605"/>
    <dbReference type="EnsemblBacteria" id="AGZ44979">
        <property type="protein sequence ID" value="AGZ44979"/>
        <property type="gene ID" value="AFR_33605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6801"/>
    <dbReference type="eggNOG" id="COG3485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027719_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="SSF49482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="INTRADIOL_DIOXYGENAS" evidence="6">
        <location>
            <begin position="108"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="30138" checksum="BB0B1877707AECB4" modified="2014-01-22" version="1">MPVVQEQKDQDNAAYVRRVHDKGLQFDLRTMSRRRLLAAFGGAGALALVGGVAACDSTAEEATGTTGDLTEVESETAGPFPADGSNGPDVRTEQGIVRKDIRPSYGDASGTAEGVPLEFSLVVTDLDGAAVTGAAVYAWHCDREGNYSLYSSGVTDQNYLRGIQETDSTGKVSFTSIFPACYSGRWPHIHFEVYSSLVNATSGDGPIVKTSQIALPSTAADAVYATDGYSRSVSNLSQVSLATDTVFGDDSAATELATVTGSVTDGYLATLTIAIDGGAPGSAPPSRTAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W796</accession>
    <name>U5W796_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560158.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27800"/>
    <dbReference type="EnsemblBacteria" id="AGZ43821">
        <property type="protein sequence ID" value="AGZ43821"/>
        <property type="gene ID" value="AFR_27800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5634"/>
    <dbReference type="eggNOG" id="COG0161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_0_11"/>
    <dbReference type="OrthoDB" id="478143at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="455" mass="49996" checksum="AF4638925FA2E203" modified="2014-01-22" version="1">MTEDLSASARDHLWMHFTRMSSYTGGATEVPVITRGSGPYVWDQNGKRYLDGLSGLFVVQAGHGRSELADAAAKQASELAYFPLWSYAHPTAVELAERLAGLTPGDLNRVFFTTGGSEAVESAWKLARSYFKLTGKPMKTKAISRTIAYHGTTMGALSLTGLPLIKQEFEPLVPGAIKVPNTNYYRRPDESMSEEQFGIWAADRIAEAIEFEGPDTVACVFLEPVQNAGGCFPPPPGYFQRVREICDRYDVLLVSDEVICAFGRLGEYFGSTHYGYTPDIITCAKGLTSGYVPLGAMIASERLVEPFLQGANSFSHGVTFGGHPVASAVALANLDIFEREDLNGHVRANSALFRSYLERLTDLPIVGDVRGDGYFFGIELVKDKATKTTFDVDESERLLRGFLSKALFESGLYCRADDRGDPVIQLAPPLICEEEHFAEIEHALRGVLTEAWTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7F9</accession>
    <name>U5W7F9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_34300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561455.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34300"/>
    <dbReference type="EnsemblBacteria" id="AGZ45118">
        <property type="protein sequence ID" value="AGZ45118"/>
        <property type="gene ID" value="AFR_34300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6940"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="10"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="440" mass="46217" checksum="2DE9EBC5DEC1EAB0" modified="2014-01-22" version="1">MSVPARIGRYRVTRRLGSGAFATVWLAEDDALKSSVAIKVLADNWAHHPDVRARFEQEAQVLRRADSERLVRIHDVDELPDGRPYQVMTYAAGGTLAERLDAGPLPVREALRIAAEIAHAVTDLHESGVLHRDLKPSNILFATVRGSERLLVADLGLAKAIAHASGFTVVAGTPGYMAPEQHLPGGGLDVRADVHAVGAMTYQMLTGRTPQAGAPPPSKLRAGVSKQVDRIVLRALNAERDRRWPTAASFAAALDDARGASPFRRRSRVLLSAGLLVAVAGTVALGGSATAAEGWTRVADATGVLSVAVPDAWADQLIDGGWDPAAVGLAAGPAPGLLVGSDLPRWPDTASRNPGVFAGVSATMRGARLPVHAGCTRLPARAVTASGLTGAVQRWTNCPGTPVSYSEAALLSSGGDGVYVQIRQADDVDRTDQILAGLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7H7</accession>
    <name>U5W7H7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SARP family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_28225</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AfsR/DnrI/RedD regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560243.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28225"/>
    <dbReference type="EnsemblBacteria" id="AGZ43906">
        <property type="protein sequence ID" value="AGZ43906"/>
        <property type="gene ID" value="AFR_28225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5721"/>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004665_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15831">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005158">
        <property type="entry name" value="BTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03704">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01043">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="BTAD" evidence="3">
        <location>
            <begin position="98"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="543" mass="58234" checksum="C9A63C428E1EA7A8" modified="2014-01-22" version="1">MLFHVLGPLEVHTTIPCRPGAGKPAAVLAALLLRPDAWVGVDRLVEAVWPGRAAPASAEANLRTYVWQLRRMLPDHQDGPRIERTADAYRIRVGPGELDAQRAAELGAAARSARGTEALGLLHEALGLWRGRPFEGVEVEPEPVAQLEQLHLDLREHLGEVQLTLGRGPDAIATLRSVVADAPLRETAWTLLVRAQHTSGLRTEALVAYREAAEVFRAELGIEPGPALTAAHRLALGSTRRELPRDVHLIGRDTELARLEEPAPVVIVDGMPGVGKTALVVHAAHRLVPGFPDGQLFVSLGGSEPVAQRLLRAVGSTDVPSDPEEQAALWRSEVARRRLLIVLDGARDAAQVRPLLPATPGSRMLITTAHRGWHLDGAVRIRLQPLGEEDATALFASAAATRTLDRRVLHGCGGLPAALLDAAARLLTRPYWTPQRLADELAEDPCQVFSGAVRQSVGAALAGLSVTEQSAWRALGSAPAEFGAEPGTRAAYESLVDKGLLDSTGPDRYRSHPVLRHLAACRPARTPRHRLLALATPPIGGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Q2</accession>
    <name>U5W7Q2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44020">
        <property type="protein sequence ID" value="AGZ44020"/>
        <property type="gene ID" value="AFR_28795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5837"/>
    <dbReference type="HOGENOM" id="CLU_1727435_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665788" evidence="1">
        <location>
            <begin position="24"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16154" checksum="E955CDEC944F1094" modified="2014-01-22" version="1">MSRSQCYLAIVMSALMVGGCADGNKSRTPEPQQPSSPVPASTRASAFCLDLGTFQFAVVVYVGEAGKAIEGKPLDFEELRRQSDNIARIGKPMRADAPADIRKEFGIVLDAVATSRKALRAGGSPKDLVDPLYGSENRPAFDAVNKYDCAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7U6</accession>
    <name>U5W7U6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative cholesterol oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_34765</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561546.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34765"/>
    <dbReference type="EnsemblBacteria" id="AGZ45209">
        <property type="protein sequence ID" value="AGZ45209"/>
        <property type="gene ID" value="AFR_34765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7034"/>
    <dbReference type="eggNOG" id="COG2303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002483_2_0_11"/>
    <dbReference type="OrthoDB" id="365211at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMC_OxRdtase_N" evidence="3">
        <location>
            <begin position="4"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="GMC_oxred_C" evidence="4">
        <location>
            <begin position="443"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="526"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="551" mass="58408" checksum="557E2B5A83478880" modified="2014-01-22" version="1">MTDYDIVVVGSGFGGSVTALRLTEKGYRVGVLEAGRRFTPETLPKTSWDLRSFLWAPALGLRGIQRITLLKDIVVLSAAGVGGGSLVYANTLYQPPAPFFADPRWAGITDWARELEPHYAQASRMLGVTVQPSMTPSDEVIRSVAQDMGVGGTFRRTPVGVFFGEPGKTVPDPYFGGAGPARTGCHECGDCMIGCRTGAKNRLDLNYLYLAERAGAVIHPDTTVRALRPVEEGWRLTTSKGTFTARQVVLSAGALGTQRLLHAMKDSGVLPQLSARLGALTRTNSEALLGAQAKSVPGKPFSAGVAITSSFHPDADTHIEPVRYGPGSNAMGLLTTLLVDGGGRVPRPVRFLGQALRHPGQLLRSLSNRRWSDKTIIALVMQTLDNSLTVRRTRRGRLTTGPGHGPANPTWIPVGHEAVRRMATKIGGFPGGSVGDVFDIPMTAHILGGVTIGASAEDGVVDPYQRVFDCPGLHVVDGSVIPANLGVNPSLTITALAERAMSLWPNAGEPDPRPALGQEYQRLDPVAPRSPAVPAHAPAALRITPAAPPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7V3</accession>
    <name>U5W7V3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative diguanylate cyclase/phosphodiesterase with PAS sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_34815</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561555.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34815"/>
    <dbReference type="EnsemblBacteria" id="AGZ45219">
        <property type="protein sequence ID" value="AGZ45219"/>
        <property type="gene ID" value="AFR_34815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7044"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="469"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="540"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="5">
        <location>
            <begin position="623"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="4">
        <location>
            <begin position="764"/>
            <end position="1020"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1025" mass="110579" checksum="9C52746D099F4670" modified="2014-01-22" version="1">MPTRLAAALFGAWMVVLGVTFYAEPAWQLPVRLLAGLSAVLVIGTGIRRQRPDHRGPWHVLSAAVVLSVAGTAVFTAGPAAERAVSHLHAIGTGILLVVYLLLATVLFWFVRLRTGGRRDRGGLLDALTLTSAVALLAWTFVIGPHLRDTDLTTIERWISVAFTVGDLLCLALLTRLLTVSGRGVRAAGWLAVGILAMLLADVGFNLVAVREDLAYLAAFGRIPLYAAAGLAALSPTMAALTRPAAAPTAELGRIRLALLALASLVAPAVLVFQVRVDGEVPDLNVVAALSACTFLLVLARMAGIMGSHRLAMARERGLREASAALVSAADADEVGDAVRTAVAQLLPADVPHRVVLAMAYTEPEEPVSPETAAQSDHDRRTGTAAGLVATRDVDWAVAVRLTQFNTVLRCPLVLQDRPTGDPLVGVLHVGGPTWALLGLQRALEVLASQVALALERIALSNEVTRRNSEAYFRTLVQNTSDVILIVDDEDRVRYASPSAVHVLGGDPVGARLPDIIHPGDRPRLADVLSAVRGGEGTQEGLDFRAVGRRRTEVMLEMHCRDLRADQTVAGLVVTLRDVTERRQLERELTHQAFHDSLTGLANRVLFADRLQHALARGARDGSVVGVLFIDLDDFKIVNDTLGHAVGDQLLIAVAERISGALRADDTAARLGGDEFAALVENVQDPGAVEETATRILAALAEPIIVDGEPLQAVASIGITTTPEADTADALLRQADLALYVAKGAGKNQWRRYQSHLHDVMVERLELRSALDHAVNEGHFLLQYQPIVDLDTDEAVGFEALVRWHHPTRGVIAPDQFIEVAEESGLIVPMGRWVLDQALHTVAQWRRILPRTRQPYVSVNVSVRQFRQSGFVEQVRASLDYAGVPPQALMLEITETLLMGDDEQIWADLAVLQELGVRIAIDDFGTGYSSLSYLRQRPIDVLKIDKTFIDDMIGSEQQLALVAGIVSLAQTLNLTVVAEGIEDATHRQLLARLGCPLGQGYYYSEPLDPTEALAWLRNPLPLAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8M3</accession>
    <name>U5W8M3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Beta-lactamase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Penicillinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_30375</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560672.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30375"/>
    <dbReference type="EnsemblBacteria" id="AGZ44335">
        <property type="protein sequence ID" value="AGZ44335"/>
        <property type="gene ID" value="AFR_30375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6150"/>
    <dbReference type="eggNOG" id="COG2367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071490_0_0_11"/>
    <dbReference type="OrthoDB" id="991474at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="PTHR35333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665806" description="Beta-lactamase" evidence="4">
        <location>
            <begin position="32"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase2" evidence="5">
        <location>
            <begin position="74"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00030171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="335" mass="35960" checksum="BC41FBA098B2E2D5" modified="2014-01-22" version="1">MSANVSRRSALGLGAAAAAVASVGMSSPAQAAKSGTAQIKKVYEKEKAKAGGIWHTHIAAVDATGTIKPIVEDDADHVTYGYSVQKLAVATAVMDKIDRGQLTLGTKLDLQADIILGGSGIYHLHGVYGDEITVANFLTAMLLVSDNTAVRMCGRVVPALEINEILAAKGFVHTRVEPVANPNRFFLGPTTPREMHDLLWRLANKTLLSPESCDFLLRITRWVNGYHDGVRRLMSSKERERIATKYGADFNDLGESRHEAGIMFSTDGVPLLTYAMFAEGLGEPENYGATHPAVEAHAKIGRTMLDNLPPVPSAARARSVAPRSLPKPFHPVKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8S5</accession>
    <name>U5W8S5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560683.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30430"/>
    <dbReference type="EnsemblBacteria" id="AGZ44346">
        <property type="protein sequence ID" value="AGZ44346"/>
        <property type="gene ID" value="AFR_30430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6161"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_11"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="25578" checksum="E9AF204666EEE2A5" modified="2014-01-22" version="1">MTISGSALVTGASRGLGAQIARRLGADGWPVAVNYRSDPAGAAKVVDEITAAGGRAAAFRADVTDESEVAALVAAVTAELGPVQVVVANATGPQPLAPAQDVTWQAHLDQLTFFVKSPTLLLQAVLPGMRSLGTGRFVHIGSDSFERALPGASAYNAAKGAQIGLARTWARELGAYGITVNVVAPGWIPVERHGEVTAEDSAGYVRDVPLGRLGRPEDIADVVAFVASDAARFMTGERLTVNGGHTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8W6</accession>
    <name>U5W8W6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="5">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Aspartate transcarbamylase</fullName>
            <shortName evidence="5">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">pyrB</name>
        <name evidence="9" type="ORF">AFR_30630</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560722.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30630"/>
    <dbReference type="EnsemblBacteria" id="AGZ44386">
        <property type="protein sequence ID" value="AGZ44386"/>
        <property type="gene ID" value="AFR_30630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6202"/>
    <dbReference type="eggNOG" id="COG0540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_2_0_11"/>
    <dbReference type="OrthoDB" id="1844275at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="8">
        <location>
            <begin position="3"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="7">
        <location>
            <begin position="152"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="32891" checksum="D9939352086914D5" modified="2014-01-22" version="1">MIKHLRSAADLDADTATLILDTAGEMAALAGREVKKLPTLRGRTVVNLFYEDSTRTRISFEAAAKRLSADVINFSAKGSSVSKGESLKDTALTLQAMGADAVVIRHPASGAPHRLASWVDGSVINAGDGTHEHPTQALLDAYTMRSRLGKLDGLNVAIVGDVLHSRVARSNVLLLTTLGAKITLVGPPTLIPVDIAAALSPETKVSYDLDSVLPGSDVVMMLRVQTERMQDSYFPSAREYSRRYGLDVPRMRKLPEHAIVMHPGPMNRGMEIAPEVADSSRSTIVEQVANGVSARMAVLYLLLGGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W995</accession>
    <name>U5W995_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative HTH-type transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561913.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36605"/>
    <dbReference type="EnsemblBacteria" id="AGZ45577">
        <property type="protein sequence ID" value="AGZ45577"/>
        <property type="gene ID" value="AFR_36605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7405"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170329_1_0_11"/>
    <dbReference type="OrthoDB" id="2031076at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AsnC_trans_reg" evidence="1">
        <location>
            <begin position="6"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="77" mass="8186" checksum="5B16E84005E9D5BF" modified="2014-01-22" version="1">MVQAYILIQTEVGKARDVAAAIDKINGVVRVDAVTGPYDVVVLAEAPNVDEVGKMIVSKIQYVPGITRTVTCSVVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Q1</accession>
    <name>U5W9Q1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32235"/>
    <dbReference type="EnsemblBacteria" id="AGZ44705">
        <property type="protein sequence ID" value="AGZ44705"/>
        <property type="gene ID" value="AFR_32235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6525"/>
    <dbReference type="eggNOG" id="ENOG5032YZA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148715_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="105" mass="11197" checksum="0C94F29471BDDE45" modified="2014-01-22" version="1">MSLPPPVQAVFDATNAGDTAAFLSAFAENGVVDDWGREFHGREAIKQWSDAENIGAHSTFEVTEVSHAADAYVVTATVGGDGFNGPSHFTFQVDGDHVTRMTIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9W6</accession>
    <name>U5W9W6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a quinol + H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = a quinone + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:39663"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the F420H(2)-dependent quinone reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561043.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32265"/>
    <dbReference type="EnsemblBacteria" id="AGZ44711">
        <property type="protein sequence ID" value="AGZ44711"/>
        <property type="gene ID" value="AFR_32265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6531"/>
    <dbReference type="eggNOG" id="COG4405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114921_1_0_11"/>
    <dbReference type="OrthoDB" id="1622823at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004378">
        <property type="entry name" value="F420H2_quin_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04075">
        <property type="entry name" value="F420H2_quin_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00026">
        <property type="entry name" value="hi_GC_TIGR00026"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="16180" checksum="F70C787DC26965B1" modified="2014-01-22" version="1">MPLSGEYAPSTSGWARKQAETYEATNGERAGDIQGRPVVVLTSIGARTGLLRKTALMRVEHNGDYAVVGSLGGGPKNPVWVYNLRRNPEVELQDGDRKRDYVAREVSGDERAVWWERSVEAFPTYAGYAKKTDRLIPVFVLEPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA22</accession>
    <name>U5WA22_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561167.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32860"/>
    <dbReference type="EnsemblBacteria" id="AGZ44830">
        <property type="protein sequence ID" value="AGZ44830"/>
        <property type="gene ID" value="AFR_32860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6650"/>
    <dbReference type="eggNOG" id="COG1975">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041115_1_0_11"/>
    <dbReference type="OrthoDB" id="1879870at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003777">
        <property type="entry name" value="XdhC_CoxI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027051">
        <property type="entry name" value="XdhC_Rossmann_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13478">
        <property type="entry name" value="XdhC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02625">
        <property type="entry name" value="XdhC_CoxI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC_CoxI" evidence="1">
        <location>
            <begin position="12"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="XdhC_CoxI" evidence="1">
        <location>
            <begin position="114"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="XdhC_C" evidence="2">
        <location>
            <begin position="199"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="368" mass="38513" checksum="E34EE08264E6611D" modified="2014-01-22" version="1">MRDVLDELLRTWRAGEQAGLATVTATWSSAPRQPGAAMLVGADGTATGSVSGGCVEGAVYELCREAAESGEPRTTRFGITDDDAFEVGLPCGGTIELFVERAGFPELPLLADEVAAGRPVALLTCVEGPPGQVGGHLVVTPDGRHGSLGGDRIDDAATDDGRGLLAAGRTGLLHYGPDGQRRGSGLTVLVTSFAPAARMLVFGATDHAAAVARIGAFLGYRVTVCDARPVFATARRFPEADEVVVDWPHRYLRAESEAGRLDARTVVCVLTHDPKFDVPVLEVALTLPVAYVGAMGSRRTHDDRLKRLRETGLGPAEIARLSSPIGLDLGARTPEETAVSVAAEIVAVRWSGSGERLTATSGPIHADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA67</accession>
    <name>U5WA67_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561168.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32865"/>
    <dbReference type="EnsemblBacteria" id="AGZ44831">
        <property type="protein sequence ID" value="AGZ44831"/>
        <property type="gene ID" value="AFR_32865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6651"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053995_1_0_11"/>
    <dbReference type="OrthoDB" id="688156at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="21"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="353" mass="38617" checksum="463231FE9CB3B735" modified="2014-01-22" version="1">MHSAITVTPAQLPGLLLHVAVVRPVFVWGAPGIGKSSLVRDFAASLGLDCVTLVGTQLAAEDLIGVPELRDGRSRFAPPESIARDEPYCLFLDELNSSSPEVQKAFYSLILDRRIGTYELPAGSIVIGAGNRSSDNALARPMASALVNRLIHVHLRASHTDWLNWAMTAGIHPWIIGYLDERPDHLWAAPPKTEEAFSTPRSWHMLSDALHSYGDDPDEETLRLLAGGTLSPAHAAAFCGYLKIVRHRYGLEAILRGDAGWPKAPNDRDLLYFLAETFRVRLVKELPADKEKAPASVRRFAHRAKGLLVELSEISLECGQLVIAAEPDGAPALPAWFLVEVSRDLPRLVAART</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAJ1</accession>
    <name>U5WAJ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_39770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562541.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39770"/>
    <dbReference type="EnsemblBacteria" id="AGZ46208">
        <property type="protein sequence ID" value="AGZ46208"/>
        <property type="gene ID" value="AFR_39770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8046"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_11_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045823">
        <property type="entry name" value="TetR_C_32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19344">
        <property type="entry name" value="TetR_C_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="36"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="25403" checksum="FD3F9107A21CE086" modified="2014-01-22" version="1">MTDQSETDPPRGRRARAAAAPGRAAPATSRVRMSAAQRREQLIATGRQLFAERGYEAASVEEVASRAKVSKPVVYEHFGGKEGLYAVVVDREVRSLLDRIAAALTAGHPRDLLEQAALALLDYIEEEPHGFQVLVRESPVLSPAGNFSSVMNDVAHQVEHILGAEFKSRGIDPKFAELYSQALVGMVALVGQWWREARKPKKEMVAAHLVNLAWNGLSHLEPKPTLITRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB00</accession>
    <name>U5WB00_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561487.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34460"/>
    <dbReference type="EnsemblBacteria" id="AGZ45150">
        <property type="protein sequence ID" value="AGZ45150"/>
        <property type="gene ID" value="AFR_34460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6972"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_17_1_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="180" mass="19166" checksum="7DB43F51ED818D68" modified="2014-01-22" version="1">MRADARQNHTRLLEAAARAFAEHGPEASLKAIAKDAGVGIGTLYRRFPTREDLVEATYRNEVVRLAGTAGELLAEHPPVDALRAWMVRYADFMATKYGMAGALRAVLADEGGRMQTRALLTEALASILAAGVADGTIAAGLDPYDVLMSLGGISIVAGEKDQGDRVHRLIDLLLNGIAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBC4</accession>
    <name>U5WBC4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34925"/>
    <dbReference type="EnsemblBacteria" id="AGZ45241">
        <property type="protein sequence ID" value="AGZ45241"/>
        <property type="gene ID" value="AFR_34925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7067"/>
    <dbReference type="eggNOG" id="COG0433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031028_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="576" mass="62324" checksum="8156A5E8D7650978" modified="2014-01-22" version="1">MNEPVGRVLGTADATPLQFWTAVTPGSYLQLDDVVVTKRELPDREPVTIAGVVTQVRARHEGAQFDSDVFAIADGTLPALVQEAAEITTTRVDPELYVPPAPGAVVHRASGAARDAALHFDRMERRVPMGTGRDGVPVFLNADFLDGTRGAHVSISGISGVATKTSFATFLLYSVFRSGQLGGDAVNARALIFNVKGEDLLFLDHPNTKLDEATTAAYAKLGLPATPFPDVRVYAPPRAGDSSGAPDVQSRLTGVDSFYWTLEEFCEKRLLPYVFADADDERQQYTMIVHSVTAHLARHAIPAEGGISIDGRRLGSYGDMVDHIVDQLTDDETRSMWAGSAVNMGTVNAFARRLIGSKRDLARLIRGDLASRRPHQIKTAESAQVTVVDLHNLPDRAQRFVVGVTLKTEFDDKEKSGTGRPLLFVVLDELNKYAPREGSSPIKEVLLDIAERGRSLGVILIGAQQTASEVERRIVTNSAIRVVGRLDPAEASRPEYGFLPPAMRQRALLARPGTMFVNQPDIPVPLCVEFPFPAWATRKSESGPPPAATMRSIVQGVDPFAVVGGRGGSSDDDIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBD5</accession>
    <name>U5WBD5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 12">Coproporphyrinogen III oxidase</fullName>
            <ecNumber evidence="6 12">1.3.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AFR_35170</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 12">Involved in coproporphyrin-dependent heme b biosynthesis. Catalyzes the oxidation of coproporphyrinogen III to coproporphyrin III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coproporphyrinogen III + 3 O2 = coproporphyrin III + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:43436"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="ChEBI" id="CHEBI:131725"/>
            <dbReference type="EC" id="1.3.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:43437"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Porphyrin-containing compound metabolism; protoheme biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Coproporphyrinogen III oxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.15" evidence="6 12"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35170"/>
    <dbReference type="EnsemblBacteria" id="AGZ45290">
        <property type="protein sequence ID" value="AGZ45290"/>
        <property type="gene ID" value="AFR_35170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7116"/>
    <dbReference type="eggNOG" id="COG1232">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009629_3_1_11"/>
    <dbReference type="UniPathway" id="UPA00252"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0274">FAD</keyword>
    <keyword evidence="8 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 12" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="13">
        <location>
            <begin position="12"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ45290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="464" mass="47639" checksum="F9C66BC4AB5DBABF" modified="2014-01-22" version="1">MRKRVAVVGGGIAGLAAAVRIRDRAPADTEIIVYEQSGRLGGKLRTGELAGTVVERGAESFLIGGPDGGESAAVRFVRRVGLGDSLVHPAAVPAALAIAGRLEPVPKGTLVGVPGDLTSLGAVARPAADADRDEGRPLLAPGADIAVGALVRARYGDEVADRLVDPMLGGVYAGRADRLSLATTMPALARTARTEHTLAGAVRAAQAASVRVPGRPIFGAVAGGMGRLVAAAAGPRRPAQPRPAVRELARTSHGWRLLIGPVWEPQTDDVDAVVLAVPARPASRLISGLDLSAAAEIEELDYASVALAAFALPPGTPLPDLSGFLVPPTEGTLVKAATFFTHKWSHLHREGGPVIIRASLGRVGEEERLQHDDTVLAATAHAELGKLLGDALPPPVASWVQRWGGGLPQYAPGHLDRVALARAALATSPGLSLAGAAFDGVGIPACVASGEKAADDVLKYLEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBI1</accession>
    <name>U5WBI1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="8">2.2.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">1-deoxyxylulose-5-phosphate synthase</fullName>
            <shortName evidence="8">DXP synthase</shortName>
            <shortName evidence="8">DXPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dxs</name>
        <name evidence="10" type="ORF">AFR_35250</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D-xylulose 5-phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:12605"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561642.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35250"/>
    <dbReference type="EnsemblBacteria" id="AGZ45306">
        <property type="protein sequence ID" value="AGZ45306"/>
        <property type="gene ID" value="AFR_35250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7132"/>
    <dbReference type="eggNOG" id="COG1154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_4_11"/>
    <dbReference type="OrthoDB" id="566385at2"/>
    <dbReference type="UniPathway" id="UPA00064">
        <property type="reaction ID" value="UER00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052865">
        <property type="term" value="P:1-deoxy-D-xylulose 5-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="PTHR43322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00204">
        <property type="entry name" value="dxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transket_pyr" evidence="9">
        <location>
            <begin position="324"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine pyrophosphate binding" evidence="8">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine pyrophosphate binding" evidence="8">
        <location>
            <begin position="155"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="641" mass="68194" checksum="8F640380928B3B3C" modified="2014-01-22" version="1">MSELSDQTTAGLLPGIKTPADLKRLDPDQLTLLAAEIRDFLVAKVSRTGGHLGPNLGVVETTLALHRVFDSPRDRVLFDTGHQAYVHKMVTGRQDGFERLRQRGGLSGYPSQAESEHDLIENSHASTALSYADGISKAFALRGEERHTVAVVGDGALTGGMCWEALNNIASAKNRLVIIVNDNGRSYAPTIGGLADHLSTLRLNPGYERVLDLVKDALGSTPVVGKPVYEVLHAVKKGIKDAVSPQPMFEDLGIKYLGPVDGHDIQAMESALRRAKGFNAPVIVHAVTRKGYGYRPAEDDEADCLHGPGAFAIETGKLLAAPSVKWTNVFAEELVAIADERPDVVGITAAMAEPTGIAALQKKYPERTYDVGIAEQHAVTSAAGLAMGGLHPVVAVYATFLNRAFDQVLLDVAMHGLPVTIVLDRAGITGPDGPSHYGIWDMSVFGAVPGLRIAAPRDATTLREELREAVAVDDGPTIVRFPTGSVAADLPALRRVGPVDVLREDDRKDILLVAVGAFAKLGMEVAERVAGQGYGVTVVDPRWVRPVPIELASLSRGHRLVVTLEDGIRAGGVGDAVASMLRDAGVSVPLRDLGVPAGFHPHGTRAEILTSLGLTAQDIARDVTEWVSQLEEAAAEHQPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBK2</accession>
    <name>U5WBK2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nicotinate-nucleotide diphosphorylase (carboxylating)</fullName>
            <ecNumber evidence="4">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_41375</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562861.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41375"/>
    <dbReference type="EnsemblBacteria" id="AGZ46529">
        <property type="protein sequence ID" value="AGZ46529"/>
        <property type="gene ID" value="AFR_41375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8373"/>
    <dbReference type="eggNOG" id="COG0157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039622_0_0_11"/>
    <dbReference type="OrthoDB" id="1718114at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="PTHR32179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="QRPTase_N" evidence="11">
        <location>
            <begin position="27"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="10">
        <location>
            <begin position="116"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ46529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="30084" checksum="AB0B7D52E224F122" modified="2014-01-22" version="1">MTDGLDLAEVERIVYTALAEDLGDPPRDVTSEATIPAGQFDTAELVARAPGVVAGLPVAAQVFAATSGGTAEFDQRVEEGTRVVRGDVLAVITGPTRSLLTAERTALNLLCRMSGVATHTRRWADELAGTKATVLDTRKTTPGLRVLEKYAVRAGGGTNKRMGLYDVAMIKDNHKLAAGSITAAYRAVRSTFPDVAVQVEVTTVAEAVEAVEAGADFLLCDNMTPDLLREVVAAVGDRAELEATGNLTLATVKDYAATGVDYLSVGGLTHSSPILDIAMDLRPRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBS4</accession>
    <name>U5WBS4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40990"/>
    <dbReference type="EnsemblBacteria" id="AGZ46452">
        <property type="protein sequence ID" value="AGZ46452"/>
        <property type="gene ID" value="AFR_40990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8298"/>
    <dbReference type="eggNOG" id="ENOG5033978">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1389347_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666271" evidence="2">
        <location>
            <begin position="27"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="197" mass="21270" checksum="EBF04ED05093A011" modified="2014-01-22" version="1">MLGWCAATLTSVALAWVAMLPVLRTATPDESTLVSVEQLRESGEVEPTPQPQSSLAEPEPDPEPTNATTTTTRTPNRTKSKPPSTPPSKSPATTTPTTTPAESTTTTEDGWTVTTDGDGEKTYVRSFRVEGGQTVIRVTEDRVRLVTATPSDGFSVATVQNEPDNLAVYFNEVNHSFVIHVVWNVDKPFAEVNEIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBT5</accession>
    <name>U5WBT5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562794.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41040"/>
    <dbReference type="EnsemblBacteria" id="AGZ46462">
        <property type="protein sequence ID" value="AGZ46462"/>
        <property type="gene ID" value="AFR_41040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8307"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078789_0_0_11"/>
    <dbReference type="OrthoDB" id="1928533at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="88"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26739" checksum="EE2CC542DB9F2AED" modified="2014-01-22" version="1">MREVRPRGWWFDLVLLGGFAALTVALARGHLLTLDVQVADWLSSHRPAPVYWVLRVLNYLGQGGQVLMPITIVLACLVAWRRRSVRPLLAFAGVFVLTYVTIGPLKIWLDRAAPAFTGADREILFNPAADGPLAMSYPSGHVANALAWYGVIAVLLGALLRSLDKPALSPRAYLALRVLPPAIVFVTTTWLAFHWITDSVAGLLLGLILTRLLTRIPWDSIPLPALPNGLDRTAGLDPAPALARH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBX4</accession>
    <name>U5WBX4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rpsB</name>
        <name evidence="7" type="ORF">AFR_36220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561836.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36220"/>
    <dbReference type="EnsemblBacteria" id="AGZ45500">
        <property type="protein sequence ID" value="AGZ45500"/>
        <property type="gene ID" value="AFR_36220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7329"/>
    <dbReference type="eggNOG" id="COG0052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040318_2_3_11"/>
    <dbReference type="OrthoDB" id="1623045at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_S2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_S2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="PTHR12534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="229"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="315" mass="34262" checksum="D77BE354083B7CF0" modified="2014-01-22" version="1">MAVVTMRQLLESGVHFGHQTRRWNPKMKRFIFTERNGIYIIDLRQTLEYIEKAYAYVRDTVAEGGHILFVGTKKQAQEAIAEHATRVGQPYVNHRWLGGMLTNFQTVYKRLQRMKELEAIGDLTGTAAGYTKKETLQLFREQTKLTKTLGGLRDMTKVPSAIWVVDTKKEHIAVDEARKLGIPVIAVLDTNCDPDEVDFPIPGNDDAIRSAELLTKVIAAAVADGLIKRSGRNRGSDDKPEPGTVAAGEPLPEWERDLYEGADKKAADAEPAAEPAAAPAAPAAAATPGNDATPVVDAVPDNPTAVAEPVAATAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC34</accession>
    <name>U5WC34_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HMP-PP phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563104.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42595"/>
    <dbReference type="EnsemblBacteria" id="AGZ46773">
        <property type="protein sequence ID" value="AGZ46773"/>
        <property type="gene ID" value="AFR_42595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8620"/>
    <dbReference type="eggNOG" id="COG0561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_0_0_11"/>
    <dbReference type="OrthoDB" id="1374424at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="270" mass="29094" checksum="465CF77E8232B11D" modified="2014-01-22" version="1">MTKPGLPKLIATDLDGTLVRSDDTVSAYTHEVLDRVRAAGIRIVGATGRGPRLTELTRNDIRAADFLVLAQGGWVIDQGENQVLRSARLPGRDLSVVLENLEAEVGPLSVMVEALEHNDAPLWGDYDPTWRYPVVLERRSRAECLTGDVIKAFARSFDHHVDDLLAAAQRIVPADVASVTQAGLDYVEICPPGVDKGTGLAVVAQAVGVDPADVLVFGDMPNDLPMFAWAGWSRVAVQNAHPTLLAVADEVTLSNDEDGVAVYLDQLLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC88</accession>
    <name>U5WC88_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acetyl/propionyl-CoA carboxylase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562909.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41615"/>
    <dbReference type="EnsemblBacteria" id="AGZ46577">
        <property type="protein sequence ID" value="AGZ46577"/>
        <property type="gene ID" value="AFR_41615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8422"/>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_0_0_11"/>
    <dbReference type="OrthoDB" id="123223at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="PTHR22855"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="483" mass="50977" checksum="FDBEECC69072798B" modified="2014-01-22" version="1">MTVLDTSIDPRTPAYLESRSAMLERLAELEAALEAARAGGGEKWVTRHHARGKLLPRERVEMLLDQDSPFLELSPVAAWGSDFPVGASVVTGIGVVEGVECVVIANDPTVDGGAVNPYTVEKIRRAARIASMNRLPLVNLVESTGAAAPSGPPPGGAIARDLSQLTADRVPTVCVVFGATTGDAAYLPALSDYTIMVRGHAKMLTIHPQPVRAAGANGRPAPEVRSAPTVPAGVTGPADQLAEDERDGLRLARQCVRRFNWRKRGPAPRAWPPIPPKYDAEDLLTLAGADPSTVFEPHEVLARILDGSDFDEFKPSQGTALVTGWGELHGYPIGVLANLGGAFSPAETQKAVHFIQLANATATSLLFVQYTGAAAAEAHEAADVRHGAPLVHAVAKSTVPHLTLMIGGTPAEEAGGIYGRAYEPRFLFSWPVERPAEGSSPRGKLPDDGVIDPRDTRTVLGLCLSAVHSAAVEGAEHVGVFRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCF0</accession>
    <name>U5WCF0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polynucleotide adenylyltransferase/metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_42900</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563164.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42900"/>
    <dbReference type="EnsemblBacteria" id="AGZ46834">
        <property type="protein sequence ID" value="AGZ46834"/>
        <property type="gene ID" value="AFR_42900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8679"/>
    <dbReference type="eggNOG" id="COG0617">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015961_6_1_11"/>
    <dbReference type="OrthoDB" id="2018439at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014065">
        <property type="entry name" value="tRNA_adenylyltransferase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02692">
        <property type="entry name" value="tRNA_CCA_actino"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HD" evidence="4">
        <location>
            <begin position="262"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="476" mass="52952" checksum="6E154D1099AAF736" modified="2014-01-22" version="1">MSELNKAQRNAVAELLRVSPVADELGRRFGAAGHELHLVGGSVRDALLGRLGDDLDFCTDARPEQTLALVKGWADAIWETGREFGTIGIQKDGLRIEITTFRAEAYDGVTRNPVVEYGDNLLDDLRRRDFTINAMAVSLPGHDFTDPFGGLEDLAAGVIRTPDSPALSFGDDPLRMLRAARFAAKLRFTVDKPVVEAMTEMAADLDRITAERIRDEFTKLLCGLDPIAGLRLLVDTGLADRFIPEISGLKLEIDEHAQHKDVYEHTLIVVSNAIRLEGDAGPDFVLRMAALMHDVGKPATKAVGRDGRVSFHHHEVVGARLTKQRMKALRYPKDVTSEVVELVALHLRFYGYGRGEWTDSAVRRYVTDAGELLPRLHKLTRSDCTTRNRRKAAALSADYDALEERIDRIAAEEDLARVRPDLDGNAIMELLGVPPGPIVGQAWRFLKELRLDRGPLDRDEAEAELQRWAREHGHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCI7</accession>
    <name>U5WCI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coagulation factor 5/8 type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42140"/>
    <dbReference type="EnsemblBacteria" id="AGZ46682">
        <property type="protein sequence ID" value="AGZ46682"/>
        <property type="gene ID" value="AFR_42140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8527"/>
    <dbReference type="eggNOG" id="ENOG5030HYY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043495_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666514" evidence="1">
        <location>
            <begin position="29"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1996" evidence="2">
        <location>
            <begin position="141"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="386" mass="40962" checksum="8199777EAB23EE6F" modified="2014-01-22" version="1">MSKHASRKQGLTVVGACLAVGVIAVAGAAVYLGAEPGPTSAVAQSVPYAPPSAGGSTATTAASAVPSAVPSVSTTASKKPKKAAAPVVETPKGGWVPVDQAAWKAQVSAYKGRKTDPVPAGVGNLPEFRADCKYSHRLADDPIVLPGLPGASHMHSFVGNKALDADTKAGDLMKFTATTCKPVEDHSAYWVPTLYDNATNKPVETTGFRVYYRSLRNNSAGQMPMPNGLRMVVGDAKKKVPTPRGAQGQFYCAFYGPGDLDGIARSTNGNWPICGQPATLHFMMQFPDCWDGKHLDSPNHKDHVVFGSDSACPRSHPVRIPAITFDIQYGAKGTKAGYYLSSDKTGKSASSMHGDAFVMWDADAMNKRTKNCILQRKTCDNYGYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCK0</accession>
    <name>U5WCK0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GSDH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_36960</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36960"/>
    <dbReference type="EnsemblBacteria" id="AGZ45646">
        <property type="protein sequence ID" value="AGZ45646"/>
        <property type="gene ID" value="AFR_36960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7477"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012253_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666299" description="GSDH domain-containing protein" evidence="2">
        <location>
            <begin position="41"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="GSDH" evidence="3">
        <location>
            <begin position="72"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="392" mass="40824" checksum="A1C09C3FF42EE86C" modified="2014-01-22" version="1">MIERSPRVRARAVTRGAVALVAVLALTAACSFGPPEPDVAGEPPNLPTPSVSAGPDSDGDRAAAATVLAKGLEVPWGLAFLPDGGALVTERDTGRILRVGPESDAEGLKVVEVQRLSEAEAGGDGGLLGIAVSPKFADDNTAYVYYSTAKDNRVAKLQLKGKPQPILTGIPRSENDNGGQLAFGPDNFLYVTTGDGTAAGTQAQNPKNLGGKILRITTAGKPAPGNPVKDSPVWSSGHRNVQGIAWDANKKMYAGESGQKTNGELNVIQKGKNYGWPTVDGKGTNTKFTNPLVTFPIEDSYCSGVAVLESMIATACLLGQRLWMVDVTGNGTLLGQPRALLTGEYGRLRALVAAPDGSFWVTTSNQEEGGEPGPDDDMIIRLVFSDGGAGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEE7</accession>
    <name>U5WEE7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40805"/>
    <dbReference type="EnsemblBacteria" id="AGZ46415">
        <property type="protein sequence ID" value="AGZ46415"/>
        <property type="gene ID" value="AFR_40805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8261"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_870532_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="319" mass="34313" checksum="F80F74F6E8A18175" modified="2014-01-22" version="1">MAALLGGLAGVAGVAGAATLGFAVARRPRAYLPAGHALVGPQIADPLPGVLRRGLAGITVPVRPGPHGELYLGPGNPQPGRTLRRLVLSPLFARAHARGGRLCRDQRVPFRLVVEFTGPTRDADLLLRAYRMLDQQLRDHAPLLSRCADGQLDPGAVTVSITGIVDVRDLLADQRRRYAFADGSFDDIGTASAPPALVPMISEPWGRRFGWDGREPISAEERHLLHALVRSAHEDGRTIRISGLPEGSRRARRAIWTELGAAGVDVIADFDQVGLARHLRRHPVGRQAEPRTPSVATPGRRVTPTPRATNPRPDIAQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF21</accession>
    <name>U5WF21_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_41830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562951.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41830"/>
    <dbReference type="EnsemblBacteria" id="AGZ46620">
        <property type="protein sequence ID" value="AGZ46620"/>
        <property type="gene ID" value="AFR_41830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8465"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_13_2_11"/>
    <dbReference type="OrthoDB" id="498436at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022324">
        <property type="entry name" value="Bacilysin_exporter_BacE_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01988">
        <property type="entry name" value="EXPORTERBACE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="203"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="400" mass="41430" checksum="0C04455127FAD94D" modified="2014-01-22" version="1">MTRFGKLWAGQTISQFGDRISELALPIIAVLSLAATPAQVGLLTAAVWLPNLLSLLVGSWVDRQAHRRRLLVAADVLRAAVLLSLPIAHWLDVITLAQLFIVALLSGAGQVLFSAAYPSFFVALVDRSGYVDANSKLSMSRSASHVAGPAVGGFLIQVLTAPVAMLVDAVSFLFSALLISRIPVSPVRVEPRRSLLVDARDGLTYVLTHSYLRAGLACVTTVNFFGFVAQALVVLFATRTLGLPVGVLGLAMGLGALGGLAGAALAPRLSRRFGLGRMLLLGAVLFPAPTAAIALAGGPVWTAATVLGLAEAVAGFGVMLLDINLNSIQAAVMADDMRSRVAGVFGTINYGVRPLGAVVGGLLGGTIGLRPTLIVAAAGGMLGALWLIPSPLPRVQEVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNL4</accession>
    <name>U5VNL4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00475"/>
    <dbReference type="EnsemblBacteria" id="AGZ38384">
        <property type="protein sequence ID" value="AGZ38384"/>
        <property type="gene ID" value="AFR_00475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.97"/>
    <dbReference type="HOGENOM" id="CLU_217443_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="45" mass="5277" checksum="12BBA50019DA1BE7" modified="2014-01-22" version="1">MSTIERIRNRRNAKRDARAIERALSSAPTQSMRNEIAIFSQQRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNZ7</accession>
    <name>U5VNZ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357611.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01895"/>
    <dbReference type="EnsemblBacteria" id="AGZ38668">
        <property type="protein sequence ID" value="AGZ38668"/>
        <property type="gene ID" value="AFR_01895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.383"/>
    <dbReference type="eggNOG" id="ENOG502ZCPR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130252_0_0_11"/>
    <dbReference type="OrthoDB" id="1735165at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665899" evidence="2">
        <location>
            <begin position="33"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="176" mass="18768" checksum="52F3F72771F5D779" modified="2014-01-22" version="1">MRTARSAPLLIVLSFVVTLGGCAGGPAPRAWAASVCTVLSPWRSEIGTLTTRTQQQMTAETTPAQAKENLMRLFGGAESASEKARAGVQKAGVPDVDEGKEVADSFTTSLSAMRDAYGRARTGVEGLATSPSKDFYSKVQAVVATLNTEYKESELDTSRLDSEELKKAFDEAPECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPX6</accession>
    <name>U5VPX6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sugar ABC transporter solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357805.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02865"/>
    <dbReference type="EnsemblBacteria" id="AGZ38862">
        <property type="protein sequence ID" value="AGZ38862"/>
        <property type="gene ID" value="AFR_02865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.576"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_9_0_11"/>
    <dbReference type="OrthoDB" id="1303718at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665559" evidence="1">
        <location>
            <begin position="29"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="453" mass="48699" checksum="3272E517CD0D30CF" modified="2014-01-22" version="1">MQKPGKKLSFLIAGAAASALVLSGCSGAGSTGGGDAADNTITVLMVGNPQMEDIQKLTADNFTKTSGVTVKFTILPENELRDKVTQDVANQGGQYDAVTVGAYEVPIWAKNGWLHELSTQAGADSAYDTADLIKPMVQSLTGDDGKMYAAPFYGESSFLMYNKEMFEAKGLTMPEKPTWAQVADFAAKLDDKNKGVAGICLRGLPGWGEMFAPLTTVVNTYGGTWFEKDWTPKVNAPEFKEAANFYVDLVKKHGESGAPQAGFTECLNTFGQGKAAMWYDATSAAGTLEDPNSSKVAGKVGYAYAPVNKTEYSGWLWTWAFAMPKTTKKQDNAWKFISWATSKDYEKLVGEKLGWSRLPSGKRTSTYSIPEYKESAKAFADITLGSIENANPTNPGLQPRPALGVQFVGVPEFADLGTKVSQELSASLAGSKTVDQALNDGQKLAEEVAKNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ21</accession>
    <name>U5VQ21_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357847.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03075"/>
    <dbReference type="EnsemblBacteria" id="AGZ38904">
        <property type="protein sequence ID" value="AGZ38904"/>
        <property type="gene ID" value="AFR_03075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.619"/>
    <dbReference type="eggNOG" id="COG0586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_4_2_11"/>
    <dbReference type="OrthoDB" id="1928533at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="228" mass="25186" checksum="4F747FFDA3F7CC48" modified="2014-01-22" version="1">MPPPDDLPSFLHSVAPILDRWGYLAVAGVIGVESFGVPAPGQTIMVAAAIYSSWGRLDIFAVAAISFVAAVLGDNVGYWIGVRGGRRAVHRFGKYIFITPARLERAEKFFARRGNRIVVVARFIDGLRQLNGVIAGITAMPWRTFLLYNAIGAALWVGWWTTIAYLLGTHLVEIIERAEEYKFWAIGAIVIAVGTYIWLHLRHIRKRRARQAAEAAEAAACEPEDVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ22</accession>
    <name>U5VQ22_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357855.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03115"/>
    <dbReference type="EnsemblBacteria" id="AGZ38912">
        <property type="protein sequence ID" value="AGZ38912"/>
        <property type="gene ID" value="AFR_03115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.627"/>
    <dbReference type="eggNOG" id="COG4894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108507_1_0_11"/>
    <dbReference type="OrthoDB" id="1864379at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="entry name" value="PTHR31087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="SSF54518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="19376" checksum="6294D8E24B735AC5" modified="2014-01-22" version="1">MYVIRERFFSIGDDFDVLDEHGTKLFHVDGKVLSVRNKVVIEDPSGTEVASVHRHLVALRPTYEIRIGGEKAAEVKKKLFTPFRDKFTIDVPGPEDLTMKGDLLDHEYVIEREGREVAAVSKRWLTIRDTYAVQVGAGEDPLLIIASVLALDLALERDEKKKEEKDEKDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQB6</accession>
    <name>U5VQB6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdopterin biosynthesis-like protein MoeZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_04145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358063.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04145"/>
    <dbReference type="EnsemblBacteria" id="AGZ39118">
        <property type="protein sequence ID" value="AGZ39118"/>
        <property type="gene ID" value="AFR_04145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.837"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_1_2_11"/>
    <dbReference type="OrthoDB" id="1408716at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="301"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="393" mass="42464" checksum="0486CAD95DDFB48C" modified="2014-01-22" version="1">MSLPPLVEPAPELSVDEIRRYSRHLIIPDVGMDGQKRLKNAKVLAVGAGGLGSPALLYLAAAGVGTLGIIDFDTVDESNLQRQIIHGMSDVGRPKAESAADSIREINPLVNVVIHNTALDRDNVKDIFSQYDLIVDGTDNFATRYMVNDAAVLLGKPYVWGSIYRFDGQASVFWEEHGPCYRCLYPEPPPPGMVPSCAEGGVLGVLCASIGSIQVNEAIKLITGIGEPLVGKLMVYDALEMEYRKIKVRKDPNCALCGENPTVTDLLEDYDDFCGAVSEEAQEATLNATITARELKDWQDSGKDLFLVDVREPAEFEIVSIPGATLIPKGEILSGEALSRFPQDRQIVLHCKSGVRSAEALAALKAAGFKDAVHVQGGVVSWVNTVDPSLPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQM7</accession>
    <name>U5VQM7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358037.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04015"/>
    <dbReference type="EnsemblBacteria" id="AGZ39092">
        <property type="protein sequence ID" value="AGZ39092"/>
        <property type="gene ID" value="AFR_04015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.811"/>
    <dbReference type="eggNOG" id="COG3883">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073856_0_0_11"/>
    <dbReference type="OrthoDB" id="1180964at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665638" evidence="1">
        <location>
            <begin position="32"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="35977" checksum="B0745A882FBE5118" modified="2014-01-22" version="1">MAATLLRRLQPVLALFAAIAVLVVAAPAAHADPDEEGGTKTLRSALESAAKGHIEAKAKLESSKKRQVKLNETLKQSKLKATTLEARVGEIANRSYRQGRFNTMTLLLNSTSPDTFMERALRLDQMAQVDGKALTGYQDAVVTAQQAKTAIDLEVKEQKKQVTVMAKKKQQAEQALASVGGGAVAGGFINANSPLAKAAPRNSDGSWPKESCTVNDPTTSGCITPRLLNSYQQSTNAGFKRYTSCFSERASGEHPKGRACDFSAAKGGFENVNASGGDRTYGNNLAAYLVKNASRLGVLYVIWYRQIWHPGTGWRAYSGSGGPAATHTNHVHLSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQV8</accession>
    <name>U5VQV8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative exopolyphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358889.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05345"/>
    <dbReference type="EnsemblBacteria" id="AGZ39358">
        <property type="protein sequence ID" value="AGZ39358"/>
        <property type="gene ID" value="AFR_05345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1082"/>
    <dbReference type="eggNOG" id="COG0248">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025908_1_2_11"/>
    <dbReference type="OrthoDB" id="1862004at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx-GppA" evidence="1">
        <location>
            <begin position="16"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="31634" checksum="6D829B55BEBD7C5C" modified="2014-01-22" version="1">MRVAAIDCGTNSIRLLIADVGDGRLSEVSRRMEIVRLGEGVDRTGRLAPEAIERTRKALLGYAMEIAELGVDRVRMCATSASRDAANAADFTDMVRATLGVDPEVITGDEEARLSFAGAVHGLEAEAPYLVVDIGGGSTEFVVGSTEVKHAISMDIGCVRMTERHLHSDPPTPAEVAAAEADITRAVDTALRTVSGHGTATLVGLAGSVTTVAALALHLPGYDATRIHHARVPYDEVAKVTFDLLGATVADRLDLPVMHPGRADVIGAGALILRIIMERAGKTSVVASEHDILDGIAFSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR81</accession>
    <name>U5VR81_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PadR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358890.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05350"/>
    <dbReference type="EnsemblBacteria" id="AGZ39359">
        <property type="protein sequence ID" value="AGZ39359"/>
        <property type="gene ID" value="AFR_05350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1083"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_3_1_11"/>
    <dbReference type="OrthoDB" id="1706427at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="16"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="109" mass="12158" checksum="286B62AD5301EF8A" modified="2014-01-22" version="1">MDTTQLLKGVLDLACLAALRDEDGYGYDILRRLRTAGLTDVGDASVYGTLRRLFNAGLLTTYVVPSDEGPHRKYYALNAAGRAELQRFSKTWHGFASTMDDLLRERETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRY0</accession>
    <name>U5VRY0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357443.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01055"/>
    <dbReference type="EnsemblBacteria" id="AGZ38500">
        <property type="protein sequence ID" value="AGZ38500"/>
        <property type="gene ID" value="AFR_01055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.210"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_13_11"/>
    <dbReference type="OrthoDB" id="1268974at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="16390" checksum="7EDC45DFAE0B1E90" modified="2014-01-22" version="1">MSRAVEELNRRLLRARDTIDRAYAEPLDIRAIAAVAHLSPAHFSRCFRAVFGETPHRYLQRRRIERSMFLLRETGRSVTDICSDVGFTSLGTFSRTFREIVGETPSGYRRGHGPVVTPHCVQLVNMRPRQDRAVSDKPLTGPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRZ9</accession>
    <name>U5VRZ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357458.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01130"/>
    <dbReference type="EnsemblBacteria" id="AGZ38515">
        <property type="protein sequence ID" value="AGZ38515"/>
        <property type="gene ID" value="AFR_01130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.225"/>
    <dbReference type="HOGENOM" id="CLU_2191326_0_0_11"/>
    <dbReference type="OrthoDB" id="2132515at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="106" mass="12283" checksum="31409E52CDA5B3BC" modified="2014-01-22" version="1">MIYDVYGYGEPGLGDQPQRLAEALGVEWALHESDYRGSYRAARIGTGKLRLQSNDLRDGEDRYHQEPGFPECRYLLFVDKSDRPDEVRSRLASLPQWRFLYRSVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS21</accession>
    <name>U5VS21_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zeta-phytoene desaturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_00885</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 3">Carotenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the carotenoid/retinoid oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00885"/>
    <dbReference type="EnsemblBacteria" id="AGZ38466">
        <property type="protein sequence ID" value="AGZ38466"/>
        <property type="gene ID" value="AFR_00885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.176"/>
    <dbReference type="eggNOG" id="COG1233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019722_2_1_11"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00612"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014105">
        <property type="entry name" value="Carotenoid/retinoid_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02734">
        <property type="entry name" value="crtI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="4">
        <location>
            <begin position="15"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="492" mass="52817" checksum="B4CAF15DFC330BCE" modified="2014-01-22" version="1">MTGPTDHVVVVGAGLGGLACALHLAAAGRQVTVVEREAVPGGRAGRLSLGGYEFDTGPTVLTMPELIAEPLAAVGEELSDWLELMPVNPAYRAYYPDGSTLDVRTDTVEMAAEISKVCGPREADGYLRFVDFARGLWHLERDNFIDRNLDTPLDLVNLSLLKLLAKGGFRRLQPKINQYFRDPRTQRIFSFQAMYAGLAPHDALAIYAVIAYLDSVAGVFFPRGGMHAVPKALAGAAEKHGVQFRYETTVTGVETSNGRATGVITDTGERISADVVVLNPDLPVAYRDLLPESRRPRKLTYSPSCVVLHIGSTQTYSKIAHHNIHFGKAWKGTFNEIIDQGLLMSDPSLLVTNPTRSDPSGAPAGKQSYYVLAPTPNLDKAPLDWKGGLGRRYADELAGVLEQRGYIGFRDGIEVEQIITPDDWAAQGMAAGTPFAAAHTFAQTGPFRPANLHPTLGNVVFTGSGTQPGVGVPMVLISGKLAASRVTGGAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS57</accession>
    <name>U5VS57_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Two component signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_06715</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359163.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06715"/>
    <dbReference type="EnsemblBacteria" id="AGZ39632">
        <property type="protein sequence ID" value="AGZ39632"/>
        <property type="gene ID" value="AFR_06715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1372"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_6_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="3">
        <location>
            <begin position="314"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="407" mass="44252" checksum="5C5A7D3EA372361F" modified="2014-01-22" version="1">MWRERAGVGQWVYQVLYRLLLLLYAGVVAGLCAVVVTWPHRPIASLADIPWWSHTIALVLVAVTWAPVSGQISRGVHQLAFGERDNAYEVVERVHQHLHAGPGPDELLPTLAATLAHTLKLPYVSIETGEDTVAAYGSMGDGMQVVRIPLRYQSLTLGTLSVSARRRFDGLSSADLRLLDDLAGQVGITLYAARLSEELQTSREKLVTAREEERLRIRRDLHDGLGPTLASLGLQLGALHRTLRTDSDGAERLVKELRDDVREATAEIRRLVYELRPPMLDEFGLIDALRNLRPVDGLSRSIRAPGPLPALPAAVEVAIYRIAAEALHNAARHAEAGRCVVELTGRDDTVTLTVTDDGRGLPADYLAGVGHRSMRERATELGGSITIVPAGTGGTRVTATFPIKGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS60</accession>
    <name>U5VS60_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MerR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359168.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06740"/>
    <dbReference type="EnsemblBacteria" id="AGZ39637">
        <property type="protein sequence ID" value="AGZ39637"/>
        <property type="gene ID" value="AFR_06740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1377"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065103_2_0_11"/>
    <dbReference type="OrthoDB" id="1748122at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="31033" checksum="7A800A92D5C56B2A" modified="2014-01-22" version="1">MLSIREFSEMCHLSPQALRFYHAEGLLVPAETDERTGYRRYAFEQVEQAMLITLLRDTGMSVKLVRQALDEPDRATTLLARHSDEVHCQRAAQDEAIQAARSVFEARPEPQESHVPARTVIARLVPGTPVGRDAQEWEEAERIIAETVTELLDTVEKAGATCLGRPWRTLALETPEQDRRALDGEGPFWIVKVAVTAPDEVLAALPAGFLVRNEEAGDELSIFMPGRNTMAKYCTVILRLLNAQPVDGDSFVNVARVRQELRPDGVLSSAPVERRPTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSG7</accession>
    <name>U5VSG7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39809">
        <property type="protein sequence ID" value="AGZ39809"/>
        <property type="gene ID" value="AFR_07600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07600"/>
    <dbReference type="HOGENOM" id="CLU_1954883_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="128" mass="12982" checksum="4D526E078CDE9DE6" modified="2014-01-22" version="1">MNFGRGVVVRRAGAGSSGGPGRGGLGSDSFARGGLLFADLPRGRFQVAQQVVVGGSGSGSLRFGVEEAARAVTGAFGCGPGGRGAGRVGVDRRCHHVIGHGGSGLRLELRGWFRVAVQGQVDLRIVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSU8</accession>
    <name>U5VSU8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4097 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359418.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07990"/>
    <dbReference type="EnsemblBacteria" id="AGZ39887">
        <property type="protein sequence ID" value="AGZ39887"/>
        <property type="gene ID" value="AFR_07990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1631"/>
    <dbReference type="eggNOG" id="COG3595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076844_0_0_11"/>
    <dbReference type="OrthoDB" id="1508494at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025164">
        <property type="entry name" value="DUF4097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13349">
        <property type="entry name" value="DUF4097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4097" evidence="1">
        <location>
            <begin position="40"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="26327" checksum="037B94F184CD56D4" modified="2014-01-22" version="1">MTPTLARRAGAATLIAATAAVLAGCGGGIGARLTYDDTEKVKVSEIVLTGGSGDLTVRTAAVSETRIKRIVRTDSAADPQASYRLDGTVLNIDTSCGESCRVSYEIEAPTGVAVRGKLGSSDVALADVATVDVTVGSGDVVITGATGSVAIKTGSGDITANNLKGAVQLVSGAGDVKAVDLSGGAAVRAQASSGDVDVQMTAVAPVTARTGSGDITLLVPDGAYQIKTHTGSGDEAVVGLTNDPAAKNLLDVQTGSGDATITALPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTA6</accession>
    <name>U5VTA6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357913.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03405"/>
    <dbReference type="EnsemblBacteria" id="AGZ38970">
        <property type="protein sequence ID" value="AGZ38970"/>
        <property type="gene ID" value="AFR_03405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.685"/>
    <dbReference type="eggNOG" id="ENOG50345V2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042795_1_0_11"/>
    <dbReference type="OrthoDB" id="1513329at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="386" mass="41343" checksum="605F6683A6379639" modified="2014-01-22" version="1">MNGALTGILLVVLWGTVAARLPTLWRDAQQRAMWATLCALALTKTVATPGVNSVLGELIPHPQILPHLLGVAAAYFLLRFISLITAYDAAHPRAARYQPLLAGVVVVLLIVLMNATPGGISTTGADLMAATLTPSATAYWVVLNGYLGTVLVIATALFWRISRSAAARLLRNGLRAIAMGTFLVAAYAVLKTGVIVAHGSGLTLPLQEIEPIANALRSAGTILAVVGAAVPAGGKLRSVIRSYRSLWALRPLWRVMRRTFPQVILFSRRRALVELAGVDDVQLRLYRRVIEIRDGMLTLRDYLPAGTLAEARLFVGESPALIEACGITLALHRHDSGLPPVDNGDHWAQVGGEVADEVAWLSAVAAAFRRKEPAAFTRRRTATVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTT4</accession>
    <name>U5VTT4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative OxPP cycle protein opcA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10620"/>
    <dbReference type="EnsemblBacteria" id="AGZ40413">
        <property type="protein sequence ID" value="AGZ40413"/>
        <property type="gene ID" value="AFR_10620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2165"/>
    <dbReference type="eggNOG" id="COG3429">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046988_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004555">
        <property type="entry name" value="G6PDH_assembly_OpcA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38658">
        <property type="entry name" value="PTHR38658"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10128">
        <property type="entry name" value="OpcA_G6PD_assem"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="342" mass="36556" checksum="E5D0561CEE830FDA" modified="2014-01-22" version="1">MEASMIGLWDTTGNEVVKALAAERRSAGGVASGLALTLVAVVEEKKVREAEAAATIAASAHPCRLLIVVRSDLEGRSRLDAEIVVGGRLGPAEAVVMRMYGRLALHAESVVMPLLAPDVPVVTWWHSEPPDVIANDFLGVVADRRITDCKLAPDPVAALGQRARDYAPGDTDLTWTRITLWRTLVAGAFDTTEERVTGATIVAPLNDPTAALMKGWLKSRLGIEPHWEHTDRSPLMRSVELKCANGDCVRVTREEGTALFSRTGQEDRYMPLPRRTVGDELAEELRRLDADQIYGEALGAMAGVAGLDSKPAMRVHVWKDPALAARAGDAAGAYAGPGGSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTZ2</accession>
    <name>U5VTZ2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L7/L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rplL</name>
        <name evidence="7" type="ORF">AFR_04455</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Part of the ribosomal stalk of the 50S ribosomal subunit. Forms a multimeric L10(L12)X complex, where L10 forms an elongated spine to which 2 to 4 L12 dimers bind in a sequential fashion. Binds GTP-bound translation factors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358152.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04455"/>
    <dbReference type="EnsemblBacteria" id="AGZ39180">
        <property type="protein sequence ID" value="AGZ39180"/>
        <property type="gene ID" value="AFR_04455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.900"/>
    <dbReference type="eggNOG" id="COG0222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086499_3_0_11"/>
    <dbReference type="OrthoDB" id="1822695at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_L7/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_L7/L12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_L7/L12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_L7/L12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="PTHR45987"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="SSF48300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00855">
        <property type="entry name" value="L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L12_N" evidence="6">
        <location>
            <begin position="6"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L12" evidence="5">
        <location>
            <begin position="61"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="127" mass="13170" checksum="56521A831AEB020D" modified="2014-01-22" version="1">MAKLSTDELLGAFKEMTLIELSEFVKQFEEVFEVTAAAPVAVAAAGGGAAPAEAEAEKDSFDVVLEGDGGKKIQVIKVVRELTGLGLKEAKDAVEAAPKAILEGVNKEKAEAAKAKLEGEGAKVTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU25</accession>
    <name>U5VU25_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="5">IGPD</shortName>
            <ecNumber evidence="5">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">hisB</name>
        <name evidence="6" type="ORF">AFR_10115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360066.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10115"/>
    <dbReference type="EnsemblBacteria" id="AGZ40312">
        <property type="protein sequence ID" value="AGZ40312"/>
        <property type="gene ID" value="AFR_10115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2060"/>
    <dbReference type="eggNOG" id="COG0131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044308_3_0_11"/>
    <dbReference type="OrthoDB" id="1278103at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="PTHR23133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4 5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="203" mass="22076" checksum="59A16F3A9BFE2E65" modified="2014-01-22" version="1">MSRFARIERVTNETKVLVEIDLDGTGKGDLSTGVGFYDHMLNQLAKHGGFDLTVRTEGDLEIDAHHTMEDTAIALGDAFSRALGDKAGIRRYGSATIPMDEVLVRAAVDLSGRPYVVHEEPELAPYIGPVYPTSMTRHIFESFGHAARVTLHVSVLRAARDGGKADAHHVVETQFKAFSRALREAVEIDPRNAGALPSTKGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUB2</accession>
    <name>U5VUB2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358834.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05130"/>
    <dbReference type="EnsemblBacteria" id="AGZ39315">
        <property type="protein sequence ID" value="AGZ39315"/>
        <property type="gene ID" value="AFR_05130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1038"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056519_1_0_11"/>
    <dbReference type="OrthoDB" id="1815576at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="60"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="28749" checksum="CB094CC302E9E135" modified="2014-01-22" version="1">MSALRRIGPLTVIALQDAEGPHFDRREDAIPGATGDQWAAADRFDPGARAEDGRWWLRFRSFAIRYGDSGPVTLVDAGIGPAHSLAADWAPVPGQLPEEVAAAGIAAEDVEAIVLTHLHTDHIGWAVPADSPFTNARVVVQRADVEAYAAASAWSRQEEVLLEPLRRLGRLHVVDGDTELGPGKKIIFTPGHTPGHQSVLVEGGGDSLLITGDLLVHAVQLVDPSLAYGHDMDADLARISRHAALTTARTGDSWLAVSHLGQPYCRTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUB3</accession>
    <name>U5VUB3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360372.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10900"/>
    <dbReference type="EnsemblBacteria" id="AGZ40469">
        <property type="protein sequence ID" value="AGZ40469"/>
        <property type="gene ID" value="AFR_10900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2220"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_3_11"/>
    <dbReference type="OrthoDB" id="1204073at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="215" mass="23710" checksum="1B8009E7A69025F0" modified="2014-01-22" version="1">MPLVFLALDDTLLDRSGAFRLWAKGFLDEIAAPHDDLEWLLSVDADGLTSRWDLADLIRDRYQLDVPSIDLLEELHEGPLAYERLDPLVACALQIAGDAGWVPVVVTNGPHEQQETRIRRTGLDRYVADWVISEQAGVSKPNPRIFALAAQRVRMRLGGAWIVGDSPEADIGGAAAMGLPSVWLHRGRDWIDNRFAPTRVVGNVIQGLSAVMAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUH5</accession>
    <name>U5VUH5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05010"/>
    <dbReference type="EnsemblBacteria" id="AGZ39291">
        <property type="protein sequence ID" value="AGZ39291"/>
        <property type="gene ID" value="AFR_05010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1012"/>
    <dbReference type="eggNOG" id="ENOG50308AJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123834_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="133" mass="14147" checksum="B469628579A30D06" modified="2014-01-22" version="1">MKPHRTDGISLSFGLIFLLIAGWWAITRVMSVHLPAAGWLVAGGLILFGVIGLLGAIRSGKRTEVPAPVQAEAQVETPGDLPPEMHADIVRELLDNPADRVDLHPAYLSHPVSTPPAPQSPAAPPAETDKKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUH8</accession>
    <name>U5VUH8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative polyphosphate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_05455</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyphosphate kinase 2 (PPK2) family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358911.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05455"/>
    <dbReference type="EnsemblBacteria" id="AGZ39380">
        <property type="protein sequence ID" value="AGZ39380"/>
        <property type="gene ID" value="AFR_05455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1106"/>
    <dbReference type="eggNOG" id="COG2326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048699_2_0_11"/>
    <dbReference type="OrthoDB" id="953793at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022488">
        <property type="entry name" value="PPK2-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022486">
        <property type="entry name" value="PPK2_PA0141"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03976">
        <property type="entry name" value="PPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03707">
        <property type="entry name" value="PPK2_P_aer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPK2" evidence="4">
        <location>
            <begin position="69"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="324" mass="37894" checksum="9ED931DA2D92E439" modified="2014-01-22" version="1">MQADQEHDDHNGHQWDEELTTEPYRGPIAELDGYRVADNDDDDPVLLLPDGSPVDTWREDYPYDDRMPRNDYDHDKRLLQIELLKLQNWCKRTGERMVILFEGRDAAGKGGTIKRFMEHLNPRGASVVALEKPNERESNQWYFQRYISHLPASGEIVLFDRSWYNRAGVERVMGFCTRAEYLEFMRQAPDLERMLVRSGINLIKFWFSVSQGEQRTRFAIRQVDPVRQWKLSKMDLESLDKWGEYTEAKEAMFFYTDTADAPWTVVKSNDKKRARLEAIRYVLNRFNYDDKDLSVVGTPDPQIVGPASLAVEGTEMSPRVFPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUR8</accession>
    <name>U5VUR8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359011.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05955"/>
    <dbReference type="EnsemblBacteria" id="AGZ39480">
        <property type="protein sequence ID" value="AGZ39480"/>
        <property type="gene ID" value="AFR_05955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1210"/>
    <dbReference type="eggNOG" id="COG4967">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1764066_0_0_11"/>
    <dbReference type="OrthoDB" id="1786325at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="147" mass="15926" checksum="155CBB1E7CADF65E" modified="2014-01-22" version="1">MTRKAPGDAGFTLVEVLTALGVIGVVATAVTTFFVRSMVLIDIEGTRQTAIQVASLGMEQLRSQPAATALTWLTDHRLEKVEKVQPEGADGKTFKRWWDVPAAGSLIPATVHVTWTERGCTPERCSYTTTTMISTSTLEPVFDSVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUY0</accession>
    <name>U5VUY0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360716.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11850"/>
    <dbReference type="EnsemblBacteria" id="AGZ40659">
        <property type="protein sequence ID" value="AGZ40659"/>
        <property type="gene ID" value="AFR_11850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2413"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049704_1_1_11"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="212"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="35525" checksum="2F2A0493BCF4CAA2" modified="2014-01-22" version="1">MPLVLDTSLLPAAQRAEAVREAMTSSIAPASVAVAPPGHARIAHWVLGPGAEFLHHVSSGHRLTRTSRHLQSDNPERISLGLPVSGAVRMRHRDLPGGDRIGELQLVDLTSPYDFLVEETSTVQALIVDYSHLGVPVDSLRNAIPRLAASPMYELVRRHLLELPGVLDNLQPDPALTFLGSSTVELVRGLIASAADPDASWLRAASTGTLFTRVTEYIRQHQREPDLSAARLAAAHDVSVRTLYAAFAAENEQLGEWVIRGRLREAHRELAECPTATVASIARSWGFANARHFARRFRDEYGISPGEWQHGVSRPAVVTPATNSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV24</accession>
    <name>U5VV24_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359037.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06085"/>
    <dbReference type="EnsemblBacteria" id="AGZ39506">
        <property type="protein sequence ID" value="AGZ39506"/>
        <property type="gene ID" value="AFR_06085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1236"/>
    <dbReference type="eggNOG" id="ENOG502ZN0K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399862_0_0_11"/>
    <dbReference type="OrthoDB" id="1518967at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="20347" checksum="5D9E704E0C992113" modified="2014-01-22" version="1">MIRLSTLPSTREQARRALLLIGAPAPARLVVDVHGALFDGDLSIPALAALLRDEEREFAGDAQAAYTICPALQPDLTAARGLITLSTWPVAGRITAPATDTLAAVVRTAEFVAMRETAGPAAAALLRRLAEDVPGGPEAYAVHNPVALADAARTALAETAGVPLPPGIADRWAGLERRQQLFGVLGVPQQRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVG7</accession>
    <name>U5VVG7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator CdaR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_13560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CdaR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361057.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13560"/>
    <dbReference type="EnsemblBacteria" id="AGZ40998">
        <property type="protein sequence ID" value="AGZ40998"/>
        <property type="gene ID" value="AFR_13560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2753"/>
    <dbReference type="eggNOG" id="COG3835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039160_0_0_11"/>
    <dbReference type="OrthoDB" id="1439158at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041522">
        <property type="entry name" value="CdaR_GGDEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025736">
        <property type="entry name" value="PucR_C-HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042070">
        <property type="entry name" value="PucR_C-HTH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17853">
        <property type="entry name" value="GGDEF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13556">
        <property type="entry name" value="HTH_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF_2" evidence="3">
        <location>
            <begin position="158"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="HTH_30" evidence="2">
        <location>
            <begin position="341"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="414" mass="44654" checksum="90C1C1FF34F3F0DB" modified="2014-01-22" version="1">MTERAQRRGGRRPAKPQAGDAWLIDVAEGAGRDAGAVPVELLGDYLPLLADAATHGRRAKRRELDAVELLGRRAAEQGISAGKVVQLYLSAARRLWQDLPTVVRSRDPEEVRAAAAAVLQVVDDAVATLAEGYAVARRDLVRREESLRRELIDDLLRGDSDLGTLVGRAEPFGLDLARVHQVALAAPSRRLPDADAAISALEAVVFDRLGDRDVLVATKDGLLVVLAPADAGATGRMSSGMDASNELGRLMHRELSRLHRGRPWRVAVGRAHPGLYGIARSYEEAREALTMAGRLNTNTPVINAHDLLIYRVLLRDQPAIVDLVQAALTPLVQARGGAGPLLDTLEAYFATGAVATESAKRLHVSVRTVTYRLARIKTLTGYDPADPAHRFTLQAAVLGAKVLNWPQEPLPIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVR3</accession>
    <name>U5VVR3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39810">
        <property type="protein sequence ID" value="AGZ39810"/>
        <property type="gene ID" value="AFR_07605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07605"/>
    <dbReference type="HOGENOM" id="CLU_2091552_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="116" mass="12440" checksum="15EBDFA4E64321D5" modified="2014-01-22" version="1">MAVAGGSEMALTSAEPETQARAPRRTTLTMPMPMARASMATPRATIAQLDMPRPNSITNTAMVPTTAATSFRAGAIGRPFWFATVDLPGYPSGYVAPRPVESALPGGCRSRRRRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWD3</accession>
    <name>U5VWD3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a quinol + H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = a quinone + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:39663"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the F420H(2)-dependent quinone reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361136.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41077">
        <property type="protein sequence ID" value="AGZ41077"/>
        <property type="gene ID" value="AFR_13955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2831"/>
    <dbReference type="eggNOG" id="ENOG502ZHNE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1805154_0_0_11"/>
    <dbReference type="OrthoDB" id="1994448at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004378">
        <property type="entry name" value="F420H2_quin_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04075">
        <property type="entry name" value="F420H2_quin_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="141" mass="15606" checksum="C491C5EB57A1F60F" modified="2014-01-22" version="1">MTALDHLPDRIRRAIEITPAAGTHERIVDITTIGRRTGRPRRIEIFFYRAHGATYLCSGAGGGATSWHANLLANPAFTFHLKNGVRADLPAHAFTVTDPTERADVLAAIVDDLNQPHDPGTIRPTRLQDWASSRLMRVVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWR1</accession>
    <name>U5VWR1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Endonuclease i</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_15805</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15805"/>
    <dbReference type="EnsemblBacteria" id="AGZ41443">
        <property type="protein sequence ID" value="AGZ41443"/>
        <property type="gene ID" value="AFR_15805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3208"/>
    <dbReference type="eggNOG" id="COG2356">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_673868_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007346">
        <property type="entry name" value="Endonuclease-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045939">
        <property type="entry name" value="YhcR_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33607">
        <property type="entry name" value="PTHR33607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19886">
        <property type="entry name" value="DUF6359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04231">
        <property type="entry name" value="Endonuclease_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="SSF54060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666155" evidence="2">
        <location>
            <begin position="31"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6359" evidence="3">
        <location>
            <begin position="33"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="382" mass="40314" checksum="B051702DFB04E808" modified="2014-01-22" version="1">MALLMPAILALFALTVTMFRAPAGMRPAAAATLTVAQAISNQSGTGTVRGYIVGQPTGTSTVLRSNFTGDTALAIADSRTETGTGAMLYVQITTSYRSAFGLQTNPGLIGSVLTVTGSLEAYFTHPGLKSPSAMTLGGATPTPTTPTPTPTGSTSAYYAAAAGKSGENLKNALHGIISSGVTKLSYDAVWNALKATDQDPANSSNVILLYSGLSRSKTLNGGDSGDWNREHVWAKSHGDFGTSVGPGTDLHHLRPEDVQVNATRDNKDFDNGGSTVSGAPGNKTDADSWEPRAAVKGDVARMIFYMAVRYEGGDSYPDLEVDDTTGSGTAPRLGRLSALKAWNLQDPPDAFEKRRNELIYSTYQHNRNPFVDHPEWVTSIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWS6</accession>
    <name>U5VWS6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative LysR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359620.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08985"/>
    <dbReference type="EnsemblBacteria" id="AGZ40086">
        <property type="protein sequence ID" value="AGZ40086"/>
        <property type="gene ID" value="AFR_08985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1828"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_11"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32280" checksum="6F83B27BDFA4AAD2" modified="2014-01-22" version="1">MELRHLEYFVTVADERSFTRAAARLHVVQSGVSAAIKALEHELDAALLERTSKRVTLTDAGAALLPKARAALDAARAARDAVDEVRGGLRGTLRIGTLTSIGLVDQPALLGRFHRAHPDVILQLTVSTRGSEGLVDALTDGSLDVALVSVPGRAPTGVHVRQLLWHPLELVVPAGHRLTRLPVVRVEDLADERFVDFPIGYGNRTVVDRAFAAAGVDRQVSLEVIDITTGAAFVRHGLGVAILPRFAVPEDLDLVLVPVADVDLNWPLGVATSAVRAPSAAARALLGMIDDHLRPSGGLRDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWS9</accession>
    <name>U5VWS9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Esterase LipW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361388.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15235"/>
    <dbReference type="EnsemblBacteria" id="AGZ41329">
        <property type="protein sequence ID" value="AGZ41329"/>
        <property type="gene ID" value="AFR_15235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3093"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_1_11"/>
    <dbReference type="OrthoDB" id="1361074at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_3" evidence="1">
        <location>
            <begin position="85"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="320" mass="34083" checksum="285007D94381988E" modified="2014-01-22" version="1">MTTVRPPFDPELGALLAGLPPVPALDRDMLAQIRPYSHVPVEPLLEGRTVERREVTVPASDGTPLPLSIFSPAAADRVPGAPCVYWLHGGGMVMGDRFSQLDIPLEWLDRFGAVVVTIDYRLAPEVTGTTLVDDGYQGLRWIAANAAELGIDPARIVVAGISAGGGLAAGLTLMARDQGTPEIAAQLLICPMLDHRNATTSSEQFAGGPGVWSREMNAFGWESVLGDNPGRAVSGYASPAVADDLSNLPPTYVDAGTAEVFRDEDVEYASRIWAAGGRADLHVWAGGFHGFDALFPQAGLSEAARRTRTEWLARTLETGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWY5</accession>
    <name>U5VWY5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Luciferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361443.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15510"/>
    <dbReference type="EnsemblBacteria" id="AGZ41384">
        <property type="protein sequence ID" value="AGZ41384"/>
        <property type="gene ID" value="AFR_15510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3150"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_6_2_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019952">
        <property type="entry name" value="F420_OxRdatse_Rv1855c_pred"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03560">
        <property type="entry name" value="F420_Rv1855c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="33844" checksum="6C2356149FE3CB2E" modified="2014-01-22" version="1">MSIRLGYQIPNFTYPGIPVEDTFATVAAQAQEAEASGFDTVLVMDHFYQLPGIGAPENAMLESYTTLGALASLTSTVQLSALVTGNTYRNPALLAKTVTTLDVVSKGRAILGIGAGWFEREHHDYGFEFGTFGQRFERLEEALTIIEPMLKGDPGTLDGKWYTARGAMNNPRVRPDIPIMLGGSGEQKTFRLAARFADHMNIICDVADLARKVGVLRERCEEIGRDPATLATSFLVMGMVGENDQEIKELAETIPEDRRNRAFLGTPAQVADQIHENILGAGIDGITINLIRNGHRPGMVAQVGAALKPLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX36</accession>
    <name>U5VX36_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360013.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09980"/>
    <dbReference type="EnsemblBacteria" id="AGZ40285">
        <property type="protein sequence ID" value="AGZ40285"/>
        <property type="gene ID" value="AFR_09980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2034"/>
    <dbReference type="eggNOG" id="COG2919">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110221_0_0_11"/>
    <dbReference type="OrthoDB" id="1975421at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="20144" checksum="DF95207D7BDBDB6A" modified="2014-01-22" version="1">MSERITTARSGGRTAEQVGQGGRKPAAKTGATTGGGARGARHYPTVGATEGSAALKLTAEQQHETAARPRLRVAPPAPINAPRAPFIALVVAVVVAGVIGILLINTKTAENSFRIDTLQQQQKKLDSQQQDLENQIAANNSPGSLDAAARRLGLVKADSPAYIRLPDGKILGVPKPATGEVAVTAQDSAATSATGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXI7</accession>
    <name>U5VXI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Multiphosphoryl transfer protein (MTP)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AFR_16385</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:23880"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:9746"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:64837"/>
            <dbReference type="EC" id="2.7.3.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dihydroxyacetone + phosphoenolpyruvate = dihydroxyacetone phosphate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:18381"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.7.1.121"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361634.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16385"/>
    <dbReference type="EnsemblBacteria" id="AGZ41559">
        <property type="protein sequence ID" value="AGZ41559"/>
        <property type="gene ID" value="AFR_16385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3323"/>
    <dbReference type="eggNOG" id="COG1080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007308_3_3_11"/>
    <dbReference type="OrthoDB" id="467393at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047324">
        <property type="term" value="F:phosphoenolpyruvate-glycerone phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008965">
        <property type="term" value="F:phosphoenolpyruvate-protein phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00367">
        <property type="entry name" value="PTS-HPr_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012844">
        <property type="entry name" value="DhaM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000032">
        <property type="entry name" value="HPr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035895">
        <property type="entry name" value="HPr-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006318">
        <property type="entry name" value="PTS_EI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004701">
        <property type="entry name" value="PTS_EIIA_man-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036662">
        <property type="entry name" value="PTS_EIIA_man-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008731">
        <property type="entry name" value="PTS_EIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001020">
        <property type="entry name" value="PTS_HPr_His_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036618">
        <property type="entry name" value="PtsI_HPr-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03610">
        <property type="entry name" value="EIIA-man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05524">
        <property type="entry name" value="PEP-utilisers_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00381">
        <property type="entry name" value="PTS-HPr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47831">
        <property type="entry name" value="SSF47831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53062">
        <property type="entry name" value="SSF53062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55594">
        <property type="entry name" value="SSF55594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02364">
        <property type="entry name" value="dha_pts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01003">
        <property type="entry name" value="PTS_HPr_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01417">
        <property type="entry name" value="PTS_I_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51096">
        <property type="entry name" value="PTS_EIIA_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51350">
        <property type="entry name" value="PTS_HPR_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00369">
        <property type="entry name" value="PTS_HPR_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665826" evidence="14">
        <location>
            <begin position="22"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-4" evidence="15">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="HPr" evidence="16">
        <location>
            <begin position="147"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ41559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="789" mass="79970" checksum="CBB38E164398B1AE" modified="2014-01-22" version="1">MVGLVVVSHSRALALAAVALATEMVHGPPVPIEIAAGLDKTTFGTDAVAIMTAVTTADQGDGVVVLMDLGSAVLSAELALELLDDDTRARVILCPAPFVEGLVAAAVTAASGADRHEVAGEAAGGSAGKESQLGAPTPDPSPGTLTAPSTTFTVTDPHGLHARPAARLVAEARAHDARVELRNSSTGSAWVPASSLSRVATLGALPGHTIEVRASGPQAEQATAAIVALASEKAPAAGAETAGRAASPGIGIGPARHLRVPDPELPDVASQGPEAELGRIEEALATVRRDLRKIRATGEAAAIFDAHLLLLDDTVLLDDVRARLAGGLAAPQAWSAATGRIAAEFAALADPYLKGRAADVRAVADQVLRALLGLPEATVPESGVIIAPDLTPAQASGLDPARVAAVVLASGSRTSHAAILARTRGIPAVVAQGPGVLDIGEGTTVAVDGSTGEVEVAPSEAVLAAYRDRAVELEARRARAGARASTPAVTREGVAVTVAANVGSAADARAAAAAGADEAGLVRTEFLFLDRDTAPTAAEQLTAYREIADALGGRRLTLRTLDVGGDKPLRYAPAAAEDNPFLGVRGLRHSLAHPALFEEQLLAIVRVAHEVPVSIMFPMVAVVPELVRARQLLDEVIRRDGGGRPSGLRVGIMVEIPAAALKTAAFAPHVDFVSIGTNDLTQYALAAERGNAALAGLADGLDPGVLRLIDAVCRGAGTRVTVAVCGELAGDEAAVPLLTGLGVRELSVAPPAVPAIKEAVRATTLDRAALDSARALDLPDAAAVRALLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYE0</accession>
    <name>U5VYE0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lactamase_B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11860"/>
    <dbReference type="EnsemblBacteria" id="AGZ40661">
        <property type="protein sequence ID" value="AGZ40661"/>
        <property type="gene ID" value="AFR_11860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2415"/>
    <dbReference type="eggNOG" id="COG2220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051050_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="17"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="28426" checksum="B860546F64E7740E" modified="2014-01-22" version="1">MTHIGGPTVLIEVAGWRILTDPTFDPPGRRYAFGWGTSSRKKTGPSLQPEEVGPVDVVLLSHEHHADNLDDRGRELLAQVPLVVTTRAGAERLGRAADGTRGLRPWDRTTLDAPARQPISVTATPCRHGPPLSRPIAGPVIGFALQWEGQEHGVLWITGDTVLFHGVREVPRRMTVDTVVLHLGAVRFPITGPLHYSLTADEAVELCTLVQPRTIIPVHYEGWSHFAQGREAVETAFAASPDHLGERLHWLPLRAVDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYH1</accession>
    <name>U5VYH1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c biogenesis protein, transmembrane region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360819.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12370"/>
    <dbReference type="EnsemblBacteria" id="AGZ40760">
        <property type="protein sequence ID" value="AGZ40760"/>
        <property type="gene ID" value="AFR_12370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2511"/>
    <dbReference type="eggNOG" id="COG0785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055985_0_0_11"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="DsbD" evidence="2">
        <location>
            <begin position="11"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="29152" checksum="1EB756FA2B0B839D" modified="2014-01-22" version="1">MLTDAPYGLALSAGVLAAVNPCGFALLPAYVSLLVTGDDPADTPTPGMALRRALTLTAAMTAGFVAVFGTFGLLAAPAADWLANRLPWVTVAIGILLAGLGGWLAAGRELPSPVPKLSRAPKLSRGVGSLLLFGMSFAVASLGCTIGPFLGVVVASFATGSVAQGVGLFVAYAAGMALVVGAIAVAVAVARQSVVTWMRRTASVLSRTAGALMFIAGIYVAWYGWYEIRIQADPTTTDLVVDTAGQIQRTLAAALDRAGPAGIAIATAVLCAVIAALALRRRRARSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYJ4</accession>
    <name>U5VYJ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase S51 dipeptidase E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S51 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360785.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12200"/>
    <dbReference type="EnsemblBacteria" id="AGZ40726">
        <property type="protein sequence ID" value="AGZ40726"/>
        <property type="gene ID" value="AFR_12200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2480"/>
    <dbReference type="eggNOG" id="COG3340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_775297_0_0_11"/>
    <dbReference type="OrthoDB" id="1851937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009326">
        <property type="entry name" value="DUF984"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005320">
        <property type="entry name" value="Peptidase_S51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39203">
        <property type="entry name" value="PTHR39203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03575">
        <property type="entry name" value="Peptidase_S51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="ASCH" evidence="4">
        <location>
            <begin position="232"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="357" mass="37708" checksum="C2409F715BC4C0FE" modified="2014-01-22" version="1">MKLLLTSAGVNNASIHEALLGLLGKPIAEATALCIPAGMYGHPWAKPTGAWEFIAGKSRQPMTELGWKSMGVLELTALPSIDRNHWVPWVEQADVLLAGGGDALFMAHWMRESGLAALLPSLGDKVWVGFSGGSMAMTPCIGDDFIGWKPPSGADEALGVVGFSIFPHVDHPDLSENTMAAAERWAATLPNPAYAIDDDSAIRVVDGAVDVITEGHWKLLSPTVSTDGLPVLELAAPGPLRDSGVAAILDGTKTAMTGLPALHESLPTVGDRFCVVNSNGDPAAVIEMTRVTTEPIGSVTAAYAHAEGRGYPDTTAWRTAHEEFFCSDIVTGFLGYTPDLTDDTPVLTQRFVLVGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYZ1</accession>
    <name>U5VYZ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cellulose-binding family II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_19580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362560.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19580"/>
    <dbReference type="EnsemblBacteria" id="AGZ42188">
        <property type="protein sequence ID" value="AGZ42188"/>
        <property type="gene ID" value="AFR_19580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3973"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3401">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002616_2_0_11"/>
    <dbReference type="OrthoDB" id="149095at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10318">
        <property type="entry name" value="RGL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041624">
        <property type="entry name" value="RGI_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034641">
        <property type="entry name" value="RGL11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43118">
        <property type="entry name" value="PTHR43118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18370">
        <property type="entry name" value="RGI_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665365" evidence="2">
        <location>
            <begin position="28"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="3">
        <location>
            <begin position="24"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="729" mass="76577" checksum="F2358A8F9DE9ABDB" modified="2014-01-22" version="1">MRTIGKIAGLGVVAIGATLLTVVDARAAAGCRVTYAVTNTWQGGFGASVEVTNLGDPVNGWTLGWTFPSGQTVTQLWNGVVTQAGSAVSVKDAGYNAALGTNTSATFGFNAASPGANTNPSSYTLNGVACTGGTTPTTPPTTPPPTTAPPPANAAGRQMERLDRGLISVRSGSGNLVSWRWLAADPADVAFNVYRGTTRVNSTPITASTNYLDAGAAAGSAYTVRAVVGGVEQAASGTALNFANGYLDVPIQNPNGSAYAANDASAADLDGDGQLEIVLKWEPLNAKDNSQSGVTDPVYVDAYELNGTRLWRINLGRNIRAGAHYTQFQVYDYDGDGDAEVAMKTADATVSGTGQVIGSASADHRNSSGYILTGPEFLTMFDGRTGAALSTVDYVPPRGTVSSWGDSYGNRVDRFLAGTAYLDGTRPSLIMARGYYTRTVVVAWDFRNGTLTRRWTFDSNSSTNSGTAGQGNHQLSVADVDADGRDEIVYGSLAIDDNGYKLWNTGLGHGDALHVGDLDPSRSGLEVFKVDEDASKPSSWFADARTGQVLWSTPAGGDNGRGVSADVWAGSAGAESWSAAADGLRGPSGSVVQSRKPGSMNFLAWWDGDPVRELLDGTHIDKYGTSSDSRLLTAANVHSNNGTKSTPSLSGDLWGDWREEVIWPTADNTALRIYATPVATDRRITTLLQDSQYRVALAWQNTAYNQPPHPSFFLGSGMATPPRPTINQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ45</accession>
    <name>U5VZ45_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cbs domain-containing membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_13570</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361059.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13570"/>
    <dbReference type="EnsemblBacteria" id="AGZ41000">
        <property type="protein sequence ID" value="AGZ41000"/>
        <property type="gene ID" value="AFR_13570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2755"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_1_1_11"/>
    <dbReference type="OrthoDB" id="2014094at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007055">
        <property type="entry name" value="BON_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04972">
        <property type="entry name" value="BON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50914">
        <property type="entry name" value="BON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="92"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="BON" evidence="2">
        <location>
            <begin position="145"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="28847" checksum="67D18D7DF73BEDFF" modified="2014-01-22" version="1">MQTWRVRDLMTTDVITAPDTASMADLVALLTERQISAVPITDRFDVVLGLVSWTDLHRKISVSSQGNGRRAGWLSRFAPSLHWPKGTAVEVMSGPAVTIGPDATPAAAAREMHRTKVGRLVVVDEQHRLRGIITRSDLLGVHSRLDAVIRDEVLQVLGGTLPIQPGAVEVSVDGGVVVIAGRTARRTTAVAAVRLTETVPGVTEVVDRLTADVDDTAAVVPERRTSSRDPLLGWWTERLPVWWREDDTGTRNAHRKSEKELAALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZE3</accession>
    <name>U5VZE3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_19910</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19910"/>
    <dbReference type="EnsemblBacteria" id="AGZ42254">
        <property type="protein sequence ID" value="AGZ42254"/>
        <property type="gene ID" value="AFR_19910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4040"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="237" mass="25221" checksum="4C53D5F761C4D5E2" modified="2014-01-22" version="1">MNAVELTHVSKSYGEIRALRDVSLSVPEGTFLAVMGPSGSGKSTLMHCAAGLDTPTGGTVVVDGADIGRLDENSRTELRRDRIGFVFQAYNLVPSLSLLDNITLPLRLAGRAPDAAWLDKLVARVGLSDRLEHRPAELSGGQQQRAAIARALVTRPAVVFADEPTGALDLRSAHEVLGLLRDLVDEFRQTVVMVTHDPAAAARAHQAVVMADGRIVDVLPRPTAADLAARLVTLGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZS9</accession>
    <name>U5VZS9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362766.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42394">
        <property type="protein sequence ID" value="AGZ42394"/>
        <property type="gene ID" value="AFR_20610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4179"/>
    <dbReference type="eggNOG" id="ENOG5032P37">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2581826_0_0_11"/>
    <dbReference type="OrthoDB" id="2083344at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="81" mass="9460" checksum="586E3860B1411414" modified="2014-01-22" version="1">MQLTTKIRDVRVNLANRRTERVAHRRLSAELAAFQTPAERAELDFILGRHTHEETRQIREILNAQDYERQRTGNGIGSYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W022</accession>
    <name>U5W022_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362920.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42548">
        <property type="protein sequence ID" value="AGZ42548"/>
        <property type="gene ID" value="AFR_21390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4340"/>
    <dbReference type="eggNOG" id="ENOG5033WEW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529414_0_0_11"/>
    <dbReference type="OrthoDB" id="1722196at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="180" mass="19130" checksum="B0EF8C0540FDC4FB" modified="2014-01-22" version="1">MTRQPDAPRHVVSSGADADTVVRRLTRDGWTVREGFALPDPAWDVTSARLVLHGRITDPENLQLAVLAAARGAGIVAVCDAETQLGRALIDDLTRLGSVHLGPGGQSAPAEPGDTVADLVPEQRALLDRLAAGDTIAAAAAAEFLSLRTANRRIAEARALFGVRTTREAVLAYLRQRRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0V3</accession>
    <name>U5W0V3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">1-phosphofructokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_22495</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22495"/>
    <dbReference type="EnsemblBacteria" id="AGZ42769">
        <property type="protein sequence ID" value="AGZ42769"/>
        <property type="gene ID" value="AFR_22495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4560"/>
    <dbReference type="eggNOG" id="COG1105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050013_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01164">
        <property type="entry name" value="FruK_PfkB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017583">
        <property type="entry name" value="Tagatose/fructose_Pkinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000535">
        <property type="entry name" value="1PFK/6PFK/LacC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="6">
        <location>
            <begin position="22"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="PfkB" evidence="6">
        <location>
            <begin position="246"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="351" mass="35070" checksum="93AD6EB0747CF10C" modified="2014-01-22" version="1">MIVTVTLNPALDLTYRVGTLVPHGTHRVSQVAERPGGKGLNVAGVLHALGEPVLATGLLGGTTGGRVTTLLAGDAVPAAFVRIAGETRRTVAVADDVDATGFWEPGPQVTPGEWAAFVAHFRDLLGTADVVALSGSLPPGVPVDAYATLVHLAAEYAVPSILDTSGEALRHGLAARPDLAKPNTTELSALLASASVPGGAAITAGRDRTFADAAPGRAGTDAGPSGARDADAGPALLEARGGGRELADAARALELGARAVVVSQGPDGLLALTVDEAWRGVPSERLEGNPTGAGDACVAALARGLRDRTPWPALLADAVALSAAAVAAPVAGAVDAATYRRLLPAVRVSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0V6</accession>
    <name>U5W0V6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_21860</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363013.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21860"/>
    <dbReference type="EnsemblBacteria" id="AGZ42642">
        <property type="protein sequence ID" value="AGZ42642"/>
        <property type="gene ID" value="AFR_21860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4431"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_11"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="9"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="123"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="229"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="380" mass="40536" checksum="E101D217FA62F294" modified="2014-01-22" version="1">MTVERILPTEEAYELLGLTREIADGELAPRVARFEEQGEFPREVLRTIGRSGLMGLPYPETDGGAGQPYEVYLQVLEILAGSWLAIAEAVSVHTLSCFPVYAFGNERQRKSLPDMLGGDLLGAYCLSEPQGGSDAASLTTRAEKDGCDWVVTGTKAWITHGGHADFYNVFCRTGGPGPTGISCVIADADTPGLLPQIPERTMGLRSSPPAQIVFDGARIPAERLVGDEGAGFRIAMQALDAGRLGIAACAVGLAQAAVDYATAYAKERQQFGKAIGEFQGIGFMLADMATQVSAARALTLAAARLKDAGRPFSIEAAKAKLFATDAAMRVTTDAIQVLGGAGYVSDHPVERWFREAKVLQIVEGTNQIQRLVIARSLTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W141</accession>
    <name>U5W141_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Endoglucanase celA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_22970</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363375.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22970"/>
    <dbReference type="EnsemblBacteria" id="AGZ42864">
        <property type="protein sequence ID" value="AGZ42864"/>
        <property type="gene ID" value="AFR_22970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4655"/>
    <dbReference type="eggNOG" id="COG2730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012932_2_0_11"/>
    <dbReference type="OrthoDB" id="478827at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665575" evidence="4">
        <location>
            <begin position="33"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="5">
        <location>
            <begin position="35"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="481" mass="51363" checksum="1D6530A3C1976840" modified="2014-01-22" version="1">MRRPRFRTQAAALAAVALVGGGLVFAGGRADAATTAYEAESATITAGVVESNHAGYLGSGFVNTDNAVGSAVQWSPQAVAGSATLRFRFANGTTVNRPMRITVNGTDVSGAVTFAGTGAWTTWQEATVTATLRAGTNTVLATATTANGGPNLDRLTVVDGATTPPPTGVTPVAQNGQLRVCGVKLCNRYGQPIQLRGMSTHGLQWYPQCVNDASLDALAGDWGADHVRLSMYVQEGGYETNPRLFTDRMHTLIEQVSARGMYVIVDWHQLTPGDPNANLAMAKTFFTEIAQRHGSKPNILYDVANEPNETPWSRIKAYHEAIIPVVRAADPDAVILLGTHGWASLGVSDGGSETDIINNPVNATNIMYTFHFYAGSHGTEYLNTLSRAADRIPVFVTEFGTQTASGDGANNFTRSQQYLDLMAQKKISWTNWNYSDDSRSGAVFTPGTCPNGPYAGTSRLKPAGVWVRDHIRTPADNFPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1F8</accession>
    <name>U5W1F8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362093.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17660"/>
    <dbReference type="EnsemblBacteria" id="AGZ41810">
        <property type="protein sequence ID" value="AGZ41810"/>
        <property type="gene ID" value="AFR_17660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3580"/>
    <dbReference type="eggNOG" id="ENOG50330BK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1101041_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="252" mass="27447" checksum="64FC487ACADBE869" modified="2014-01-22" version="1">MRHFLRHLAEMIIAMVVGMVVLAPFWPLPTDRPDVASFVMATNMSVAMAVWMWHRGHSARATAEMTAAMYASFLVFLIPWWAGLLPPAAVLTGGHVLMVPAMILTMLHRRAEYTAPHPAVERTGLLARWPTVLAVLMTIDVIVEPQSMAPWTLLVLAFAYVAIGAVRGTLRPRRTLVVQVAALVAYLVLLAAALTAGPVLSVVLVGSGWLAHAGWDLWHHRHNAVVPRAFAEWCGVLDAITGISVLTYAATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1M4</accession>
    <name>U5W1M4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363459.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23260"/>
    <dbReference type="EnsemblBacteria" id="AGZ42922">
        <property type="protein sequence ID" value="AGZ42922"/>
        <property type="gene ID" value="AFR_23260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4714"/>
    <dbReference type="HOGENOM" id="CLU_2420369_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="91" mass="10164" checksum="74D5285350C6E54E" modified="2014-01-22" version="1">MSTDIRFRTTRSLEDVAAALGAPVSLEDAEDHWEWVLADLDGVIIDITRTHTVPAGDTDTRIFRYDNQPFSAELRQHLVDRLTAAGLLTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1W4</accession>
    <name>U5W1W4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxygen-binding heme protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363803.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24735"/>
    <dbReference type="EnsemblBacteria" id="AGZ43213">
        <property type="protein sequence ID" value="AGZ43213"/>
        <property type="gene ID" value="AFR_24735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5011"/>
    <dbReference type="eggNOG" id="COG2346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103526_1_0_11"/>
    <dbReference type="OrthoDB" id="1697277at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="SSF46458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="147" mass="15712" checksum="F21E75728D15BB0B" modified="2014-01-22" version="1">MTLYEAAGGAPAMRALAADFHARCLADPVLEHPFSHTGNPEHVQRLADYWGEVFGGPPVYSASFGGHSAMLHLHAGQGAQGDLGDRFVACFVSAMDGAQLPEVPEVRAALRDYMRWATDEVMGLSPAGSVVTAGLPMPRWDFDGLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Y7</accession>
    <name>U5W1Y7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative branched-chain amino acid ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18315</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362314.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18315"/>
    <dbReference type="EnsemblBacteria" id="AGZ41941">
        <property type="protein sequence ID" value="AGZ41941"/>
        <property type="gene ID" value="AFR_18315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3717"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_2_0_11"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="295" mass="31433" checksum="BF170A8F2539402A" modified="2014-01-22" version="1">MPSGGLLFQSIILGLLLGGLYALLAAGLTLYFGIMRVVMIAHSAFLILAAYLAWWSHERLGVDPLLSMIVTVPLFFGAGVLLQRMLLSRLRPITLTMMSVLLTFAIALIIEGLLGYAFTGTQRRIQLGYGSASFGLFGARIAVVKLIAFGLAVVALAALYLLMKKTTFGWALRATIQHRDAARLVGIDTEKVAGYGFGLGLATAAIGGTALSLDTTIYPSLHWHWIGPLMAIIVVGGLGSVPGAAAAAVLLGLMQSLLQIPMGTTWAQTIFYLALFATLAFRPQGFFGGRLAQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2T6</accession>
    <name>U5W2T6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_19770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19770"/>
    <dbReference type="EnsemblBacteria" id="AGZ42226">
        <property type="protein sequence ID" value="AGZ42226"/>
        <property type="gene ID" value="AFR_19770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4012"/>
    <dbReference type="HOGENOM" id="CLU_2730841_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="7319" checksum="C09F9C54C1C0033D" modified="2014-01-22" version="1">MSYAALNTTSVTGTAKTVASQATCRAVDQAIVAYVAQNSTVPTTIRQIKPFVKGDISAYRIVKGQAAGPGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3D1</accession>
    <name>U5W3D1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364484.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26760"/>
    <dbReference type="EnsemblBacteria" id="AGZ43614">
        <property type="protein sequence ID" value="AGZ43614"/>
        <property type="gene ID" value="AFR_26760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5425"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2013880_0_0_11"/>
    <dbReference type="OrthoDB" id="1906370at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13691" checksum="45FCC8B0BD4F17D8" modified="2014-01-22" version="1">MTDGVHVGRAGVDGAANQGWLLGHFMPKGELLHSDDVEVKWGVHPPGDRRAAWATGEIRTALLVLIKGAFDIELRDRTVLLREPGDYVVWGPGEDHSWQAGDVETVVLTVRWPSLPGWRLPPSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3P2</accession>
    <name>U5W3P2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD-dependent monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42590">
        <property type="protein sequence ID" value="AGZ42590"/>
        <property type="gene ID" value="AFR_21600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4380"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="2">
        <location>
            <begin position="2"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="497" mass="53754" checksum="E445BDBA09595493" modified="2014-01-22" version="1">MIDVIVAGGGPTGMMLAAELRLHGVGVLILEKDAEPTTVVRALGMHARSIEVLDQRGLLERFLALGKKHPLGGFFAGISKPQPEGLDTAHGYVLAIPQPVTVRLLTEHAEGLGAEIRRGTEVAGLSQDDHGVTVDLTDGTQLRSRYLVGCDGGRSTVRKLAGIGFPGEASTVDTLLGEMELTASMEELTAVMTEVRKTQLRFGAGPLGNGVFRVIVPAAGVADDRTVAPTLEEFREQLRATAGTDFGVHSPRWLSRFGNATRLAERYQSDRVLLAGDSAHVHPPTGGQGLNLGLQDAFNLGWKLAAEVDGWAPAGLLESYGTERRPVAEAVLNNTRAQMELMSLEPGAQAVRRLVAELMDFPEVNRFLIEKIIAIGIRYDLGDGPELVGRRLRDLTLRNGRLYELMHAGRGLLLDRTGRLSVRGWADRVDHVVDGSEELDVPAVLLRPDGHVAWAGDNQQELLERLPRWFGRGVPDEDDPVDAAQVGGIEREVAEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3P4</accession>
    <name>U5W3P4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction histidine kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_26880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364530.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26880"/>
    <dbReference type="EnsemblBacteria" id="AGZ43637">
        <property type="protein sequence ID" value="AGZ43637"/>
        <property type="gene ID" value="AFR_26880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5447"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021898_1_0_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="593"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="662"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="682" mass="71880" checksum="A7AE712F468F2783" modified="2014-01-22" version="1">MRGVPAVFAVAGGVALAAAVVLRLAAGDPGAAGWTIVGPGPFYAAGLYAVVRRPAERTGSWMLICGALFAIEGVLGGSVLPLIADRSWAWLVVLLRQAAEVGSVVAGTALIGLFPAGRIRRTGERWVLWCVAGIGAGLVVLSAMSWPTLSAGTFRPPLYPVVSPIYVEALAPVGPVAGLASKAFFASTLLGVGMLYLRYRTAPPADRRRVRWLLLGCGSALVLWLALAAVVSAAPAASFAVAVAVLWPLTVVLILGSFLLGVSGDGVLDIDRPARRRLVYRLLWLLIAAVYTIAAAAAGVVASRYLSIGPAVLLATTAALAFQPVRRRLERLADRWVFGARLDGYAVLTRFGAVLESTPAPARLLPDLADAIRQGLDLRWVRVRLDLSTGVAGDDDGGDPELVVPLTYAGTGLGVIECGPRRDGQLLDEDRRLLGHLAGQAATVVHAAHLSTELTERMTVIREQTAELAASRARVAQARDAERRRLQRDLHDGVQQDLVVLSAKVALTRERLRRGDERAGASLAEVQTDLTALLGSLRDFAHAIHPPVLADRGLLEAVEATASRLPLEVVIEADAALRGVRYPPPIEAAGWYVVAEALTNVVKHANAARVVIGLRESGGSLVVRVGDDGRGFDLDAPRGLGLTGLADRVSIVNGTMRVDSDPARGTTVTADLPVRDEEAGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3V6</accession>
    <name>U5W3V6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363036.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21975"/>
    <dbReference type="EnsemblBacteria" id="AGZ42665">
        <property type="protein sequence ID" value="AGZ42665"/>
        <property type="gene ID" value="AFR_21975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4455"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011139_0_0_11"/>
    <dbReference type="OrthoDB" id="271104at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00609">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="2">
        <location>
            <begin position="20"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="300"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="778" mass="83041" checksum="06A3C8E19969F2A7" modified="2014-01-22" version="1">MTLSVTPMDAVELGRVRILPDAGIGALRTERGNLPLDRLDVQASISGLVARTTVTTEFVNAFDTALEATYVFPLPDRAAVTGLTMTADGRSVEAELQERAAARETYDRAIEAGQRASIAEEERPDVFTMRVGNILPGERVTVKLALVGPLPWEDGAATFRFPLVVAPRYVPGTPLPGPAVGSGQASDTDAVPDASRITPPVLLPGFPNPLRLTVGVDIDPAGLPLGEIRSSLHTVTTEGRHVTIAPGERADRDFILRLPYAGDGSGAVCVPDETADEPEGTFQLVLLPPDDTTTATRPKDVVLLLDRSGSMGGWKMVAARRAAARVVDTLTSEDRFAVLTFDHQIDRPEELGQGLSDASDRHRFRAVEHLARANARGGTELLAPLRDGLSLLADSGKRDRVLVLVTDGQVGNEDQILAEITPLIGSTRVHTVGIDRAVNAGFLGRLAALGAGRCELVESEDRLDEAMEQIHRRIGAPVLTDVTVTGTGFTVEAVPPQTVFPGVPLVVFGRYAGTPGSALSVRGRTRDDQDFAREVPVRTTAESAVTAQWARARLRELEDRYATGQRDLEPRIVATSLRFGVLCRFTAFVAVDSRVVNEGGETRRVTQPVESPSGWEPMQDVSAPAFLVAGSASPMPPPAGAPAPGFAPVRAAAARSGPKPFAAVPSGMMAAGGPRSKMAAPTSSVLTVADLKAIVEIEAKRLREAEGRPVFERRDLLADLGSRLGALLEGMSGDELKGLRDLVERIEAGGDFEQQWALARDTLAQFAPPPERKAFWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W506</accession>
    <name>U5W506_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560419.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29105"/>
    <dbReference type="EnsemblBacteria" id="AGZ44082">
        <property type="protein sequence ID" value="AGZ44082"/>
        <property type="gene ID" value="AFR_29105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5896"/>
    <dbReference type="eggNOG" id="COG4948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_3_1_11"/>
    <dbReference type="OrthoDB" id="743369at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MR_MLE" evidence="2">
        <location>
            <begin position="137"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="395" mass="42484" checksum="E94A4E4EE143770A" modified="2014-01-22" version="1">MKVTGYRSLLTKHDWGRLTGDANGVMPEPLTDVHVLILETDEGLEGIGLGAHGDIDRVFGAVEGEDPRAVTALYDRMLAWVFKTGHAGATFGAIGAIDMALWDLKAKVAGEPLWRTLGALDRFVPGYASGLDISLDDDALVALYEGFADRGFGAAKLKGGHDVTHDLRRLVAVRDVLQRNTSHPALMLDANESWNRTQAVRFIEELERTVDLAWIEEPVRRWDAAGLASVRQHIRAAVATGENLTGLEQFRPLLDADAVDIVQVGNVWGITNFLRVAAVAHSYDLPVSPVAYHANPVAHAAAAIPNHLSFEVQHLTAPIGLTIDQEIQDGGIVLGDEPGLGVRVDEKHIEAVRAANPVARLAGPHVRPPRAGLSLVPDLGPVDASGETPARWARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5K0</accession>
    <name>U5W5K0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_24650</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24650"/>
    <dbReference type="EnsemblBacteria" id="AGZ43196">
        <property type="protein sequence ID" value="AGZ43196"/>
        <property type="gene ID" value="AFR_24650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4994"/>
    <dbReference type="eggNOG" id="ENOG5030KE1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071531_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018649">
        <property type="entry name" value="SHOCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039519">
        <property type="entry name" value="YokE-like_PH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14470">
        <property type="entry name" value="bPH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09851">
        <property type="entry name" value="SHOCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="bPH_3" evidence="3">
        <location>
            <begin position="77"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="SHOCT" evidence="2">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="238" mass="25831" checksum="5360D7EFB9B31F8A" modified="2014-01-22" version="1">MDDFLQVGLGSYRLRLYPQDRQCWEDFVILGDTFLITLPEVHNMADTPLRPDIAAAKAQMPSSLGTGREVKNLEGYLWEGETVERMVGGQYGKGTGLLVVTDRRLLFVVHGMMSQQSEDFPIERISSIQWSAGMVLGAITIFASGNKAEIKNVEKNAGKALVDMVRDRVNSKGAFSAGPIAAPPAPAPTSAPAAPAASSATEKEDIIDRIRRLGELHASGILTDEEFTSKKTDLLGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5M2</accession>
    <name>U5W5M2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold-shock DNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_31215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013732668.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31215"/>
    <dbReference type="EnsemblBacteria" id="AGZ44503">
        <property type="protein sequence ID" value="AGZ44503"/>
        <property type="gene ID" value="AFR_31215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6318"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_0_3_11"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="67" mass="7347" checksum="B22BA2D20B9E8371" modified="2014-01-22" version="1">MAQGTVKWFNADKGFGFITVDGGGADVFVHFSAIQSSGYRTLEENQRVEFEIAQGQKGPQAEQVRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5U8</accession>
    <name>U5W5U8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25250"/>
    <dbReference type="EnsemblBacteria" id="AGZ43316">
        <property type="protein sequence ID" value="AGZ43316"/>
        <property type="gene ID" value="AFR_25250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5117"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041547_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="376" mass="39896" checksum="35AABCFF35B04485" modified="2014-01-22" version="1">MKVALIGANGHGHSHRRRIAELQAQGVLELVALADIQPIDDVPPGVAVFTDHRELLAATRPAVVVICTPPHTHLPIALDALDAGCDLLLEKPPVPDRQAHATLEAAAAEAGRSVQVGFQALGSYAAQQLFDAVHDGRLGRIETVATVASWRRDDAYYARAPWSGRRIVDGRPVLDGALVNPLAHAVMQCLAVAAAAGAGEPSRLEFERYRTRDIEVDDTAFARITFREGLRMLAAVTLAGEDYIPGEVIVTGTGGRAVLEYPTDRLDMPGPPAPEGRIDLLENLIAHRRDPAGVPLVAPLARTAAFTTVVQALTGPGVPVPHLIGDEFVTSVGTPPERVRVIGGINAVLREAAETLKLPSESGVVWAVPAHTENLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W643</accession>
    <name>U5W643_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AMP-dependent synthetase and ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560917.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31620"/>
    <dbReference type="EnsemblBacteria" id="AGZ44584">
        <property type="protein sequence ID" value="AGZ44584"/>
        <property type="gene ID" value="AFR_31620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6400"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_11"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="14"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="483" mass="49366" checksum="FCF6A939324F0037" modified="2014-01-22" version="1">MLDELIAGLRDDDDRPAVLGARRTGQTVVRATRGDLSRLTGGYAAALHARGLVPGETVGIAVRPGPRSLAVLLAAYHLGLRVAVLDPTAGPDVLLARLGVAKPRLVLADAAAQAVAGWAAPLARRAQLALPDLAALAPTVTIGRRLPGTAPGLAPAPGPRPPGHDGDGDAVIVFTSGTTSRPRAVVHTRASVAAGMRTVAELVRPVPDRAVLGGTFFVLVPSLTRGAPVALPARRPRTLARQTARLAPQATYLTPPQLRSALAASTPFTGRVYSGSAPVSAGLLAAVRAAGADEAWGVYALTEVFPAAVVEGTAKAAFDGEGDLVGELLPGLRARVDETGQLLLAGPNAADRYLGEEPMEWVATGDIGRVDGSTVVLGGRCKDMILRGAENIYPGLYEPALHVSGVELAVIVGVPAGDGDERVVAVVQPASGVSPEAVHAALREPLDRMGSARPDAVLLADVPLAGRSRKPDRAAASRLAASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6C3</accession>
    <name>U5W6C3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26220"/>
    <dbReference type="EnsemblBacteria" id="AGZ43506">
        <property type="protein sequence ID" value="AGZ43506"/>
        <property type="gene ID" value="AFR_26220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5314"/>
    <dbReference type="eggNOG" id="COG5278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038241_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="440" mass="45885" checksum="25DDFCF82B9EDADE" modified="2014-01-22" version="1">MSTAQLHRVAPVVADTRGGVLSRVGRLRRTSPGRLQLILAALVALALLTGLVAGLTAASASRGTDDLGNRAQPLLVEAETIYSALARADTTAAQAFLAGGLEPSGLTDLYEEDLDRAATALTSAARRTPEDGRSAEAIRALSADLPRYAALVASARANNRQGLPIGAAYLSSASTLSRESLQPQAQALFQAAQAEVNDGYADARSSWWTALLLILLAGLLGALLLAQRYLSRTTRRTFNLPLVTATAVTLILALGVGGVLIAQRAHLSEADSEGSRPVALLAEARIAALRERGDEALTLAARSGEGDLEEDFDATTKQLDGLLGDAGAMMPTGFAGQAVQSAITSHRSYVAAHDEVRKLDNGGNYDGAVKLAVGSGTTQTFTGLTTFIDRALESRKDVFSAEISDAGRGLGLLTVLGPLLALVVCGLAFAGLRTRLEEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6F0</accession>
    <name>U5W6F0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Uricase</fullName>
            <ecNumber evidence="5 7">1.7.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Urate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_31535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5 7">
            <text>H2O + O2 + urate = 5-hydroxyisourate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:21368"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="EC" id="1.7.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the uricase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.3.3" evidence="5 7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560900.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31535"/>
    <dbReference type="EnsemblBacteria" id="AGZ44567">
        <property type="protein sequence ID" value="AGZ44567"/>
        <property type="gene ID" value="AFR_31535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6383"/>
    <dbReference type="eggNOG" id="COG3648">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048151_1_0_11"/>
    <dbReference type="OrthoDB" id="1150949at2"/>
    <dbReference type="UniPathway" id="UPA00394">
        <property type="reaction ID" value="UER00650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004846">
        <property type="term" value="F:urate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019628">
        <property type="term" value="P:urate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002042">
        <property type="entry name" value="Uricase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874">
        <property type="entry name" value="PTHR42874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01014">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000241">
        <property type="entry name" value="Urate_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00093">
        <property type="entry name" value="URICASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03383">
        <property type="entry name" value="urate_oxi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 5 7" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="31504" checksum="E05959F561BA3FC0" modified="2014-01-22" version="1">MAIVLGSNRYGKAEVRLVRVTRPGDTHGLVDLNVSIALSGDLAATHLTGDNAGVLPTDTMKNTVYAFAREHGVTSPEAFGLLLARHFVTTQEQVTGARISIESYGWERLGPHSFRRAGDYVRTARIFVTPDGTQVVSGLENLVLLNSTASEFRGFPRDKYTTLAETTERILATEVDARWRHRGDSADWDESFEGAKDALVNAFVNTYSASLQQTLFSMGTRVLDSRTELAEIRLALPNKHHYLADLSPFDLENPGEVYIAGDRPYGLIEGTVTRDDAPPASSEWYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6H7</accession>
    <name>U5W6H7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560935.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31710"/>
    <dbReference type="EnsemblBacteria" id="AGZ44602">
        <property type="protein sequence ID" value="AGZ44602"/>
        <property type="gene ID" value="AFR_31710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6418"/>
    <dbReference type="eggNOG" id="ENOG50318MJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082430_0_0_11"/>
    <dbReference type="OrthoDB" id="1959030at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="22120" checksum="7D13A4390EC9F486" modified="2014-01-22" version="1">MTGGQLAVLAALGAFHGLNPAMGWLFAVARGMQELSRAVLLRSLPPIAAGHLASVAIVAAVVSATQSVVAGNVVAIGGGAVLVGFGLWRLLSARHFRWAGMRLSSAQLAGWSFLMSSAHGAGLMLLPVLATTSVTAGGGHAAHLAGAAPDGLAGTPAAALDGLFAAGVHTLAMFAVMATCALLVYEFVGLKVLRRAWFNMDKVWAGVMVAAGALTIALTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6U9</accession>
    <name>U5W6U9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Kynureninase</fullName>
            <ecNumber evidence="4 5">3.7.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">L-kynurenine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">kynU</name>
        <name evidence="7" type="ORF">AFR_33080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + H(+) + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:25143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>H2O + L-kynurenine = anthranilate + H(+) + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:16813"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the kynureninase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.3" evidence="4 5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561211.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33080"/>
    <dbReference type="EnsemblBacteria" id="AGZ44874">
        <property type="protein sequence ID" value="AGZ44874"/>
        <property type="gene ID" value="AFR_33080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6695"/>
    <dbReference type="eggNOG" id="COG3844">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_4_1_11"/>
    <dbReference type="OrthoDB" id="1474443at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00329"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061981">
        <property type="term" value="F:3-hydroxykynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030429">
        <property type="term" value="F:kynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD biosynthetic process from tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01970">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010111">
        <property type="entry name" value="Kynureninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084">
        <property type="entry name" value="PTHR14084"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038800">
        <property type="entry name" value="KYNU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01814">
        <property type="entry name" value="kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4 5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="6">
        <location>
            <begin position="95"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="409" mass="44424" checksum="CC78A4AD12E2126A" modified="2014-01-22" version="1">MTDDLLKRAEALDAADPLAHLRERFLTPDGFDVVSYLDGNSLGRPLQATAQLLDSFVREQWAGRLIRGWTDGWMEWPLQLGDRLGAVALGAAAGQVVVADSTTVLLYKLSRAAVDARPGRRKIVLDTDNFPTDRYVLEGIAAERGLSLTWIETDPATGIRPEQVAEVVDEDTALVLFSHVAYRSGWLADTAEINRIAHAAGALTLWDLSHSVGSVDVHLDEWGADLAVGCTYKYLNGGPGAPAFAYLRRELQGELRQPIQGWMGHRASFEMGPGHEPAEGARALLSGTPPILAMVPLHANLDLLAEAGIEAVRAKSVLLTGYVLDLADEWLAPLGVEVVSPRDAARRGGHVTLRRPGFEQLLESLWDNGVIPDYRRPDGIRVGPAPLSTSFAEVHRGLSVLRALLEKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Y7</accession>
    <name>U5W6Y7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33255</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561246.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33255"/>
    <dbReference type="EnsemblBacteria" id="AGZ44909">
        <property type="protein sequence ID" value="AGZ44909"/>
        <property type="gene ID" value="AFR_33255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6730"/>
    <dbReference type="eggNOG" id="COG3250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032886_1_1_11"/>
    <dbReference type="OrthoDB" id="552273at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037398">
        <property type="entry name" value="Glyco_hydro_64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032477">
        <property type="entry name" value="Glyco_hydro_64_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042517">
        <property type="entry name" value="Glyco_hydro_64_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38165">
        <property type="entry name" value="PTHR38165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16483">
        <property type="entry name" value="Glyco_hydro_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665763" description="CBM2 domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="3">
        <location>
            <begin position="25"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="542" mass="56488" checksum="99B7C5FF804EC93B" modified="2014-01-22" version="1">MRRKRTLILSVAAVVAAAGTAWIALPASAAAATAAFTKTSDWGTGWQGDVTITNGGSSAMTSWKVEFDLPAGGTIGSFWEAEMVASGSHRTFTNRSWNGNVPVGGKVSFGLVGAGGTPLNCLLNGQPCGGGGPTAPTTAPTTRPTTVAPTKPPTTAPTTKPPTTSPTTPPTTDPSVPPASSLLPVKITNNTGRNDAVFLYLLGVNLTTGKLGYVNSGGAFTNWTGGQPTPVPAPDVSIPGPANGSSTTIKMPKNLSGRLYMSFGKKLDFRLSTDGLVQPAPWAGADPNKDILFDWSEFTLNDSGLWLNSSQVDMFAVPHVVSVQGGNGVTTKTGEVKAGGRQKVIDQVKAQAGFEKTVYTRSDGTVLRVLAPGKAADGGQFSPTYLDPYITSAWNAYTGKNLTVVPFGDQPNTKFFGRTSGNVMNFTDSTGKTVASFTKPSTANVWGCDGALGAPNDLVVGPIARTLCAAMHRTTLGTLDTQPSGTAADFYKGNLTNHYSRIIHEQMVDGKAYGFAFDDVQNQESLVHSGDPRQAGIIMTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7I3</accession>
    <name>U5W7I3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_34055</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34055"/>
    <dbReference type="EnsemblBacteria" id="AGZ45069">
        <property type="protein sequence ID" value="AGZ45069"/>
        <property type="gene ID" value="AFR_34055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6891"/>
    <dbReference type="eggNOG" id="COG2409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005108_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="509"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="565"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="606"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="641"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="208"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="556"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="699" mass="74046" checksum="AF6B88251CD7D8EE" modified="2014-01-22" version="1">MAGRWVPWLVIGLWLALAVVMVPLSGKLSSVTTDRAVDTLPAGAESTKVAVLEDSLPGGEDNTFVFVYHRAGALTDADRATVQRHYNTLAKRYPPKTAAAGEDDDDSPTRLSTDRQAMVFTLDVSTTHGAPEALVGPLRDAAKDRPAGLQLEVTGPAAIDGDLDAVFDGIDLQVFLTTVIVVTVLLILTYRSPVLWFIPLVAVGAAALTSMATVYLLVKGFGIVVNDQNSALLTILVFGVGTDYALLLIARYRESLHHHENVRVAMVHALRGAAPAIVASAATVVAGLLCLLVADLNSTSGLGPIGAAGILCALVAMLTLFPALLVVLGRRIFWPAIPRFNAAVEAKPGLWGRLGTAISRRRWVATLGSLGILGVLAIGLSGNTGALREQDQFLSAPESVTGFTVLRQHFPELGGQPMTIYTRPAYQDQILDVVKRTPGVAQAAPGETSGGWTDISVFPADAPDTAAEYDTIKRVRTAVHAVNGAEAIVGGPSAENLDTEVTTSRDEKLVIPLVLAVVLIVLGLLLRAIVAPLILMATVIVSFAAAFGGSVFVFDTILGFKGVDYSVPLLAFLFLVALGVDYNIFLTSRAREETVRLGTREGMLKALSATGGVITSAGLVLAATFAVLATLPLVMLIEVGFLVAFGVLLDALLVRSVLVPALTLLIGRRMWWPSRLSRPVELPDDRRPLVDDQEPALQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7N8</accession>
    <name>U5W7N8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_34380</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561471.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34380"/>
    <dbReference type="EnsemblBacteria" id="AGZ45134">
        <property type="protein sequence ID" value="AGZ45134"/>
        <property type="gene ID" value="AFR_34380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6956"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_5_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003012">
        <property type="entry name" value="Tet_transcr_reg_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00400">
        <property type="entry name" value="TETREPRESSOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="7">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="26380" checksum="051F1EFD08E53268" modified="2014-01-22" version="1">MAVDPSRRRAARHAQPGTVPLDPAIPRTPITVERITDAALQVVATDGYDALTIRSVAKVLGTGPSSLYAHIVNKADLDDLLVGRLCSQIKLPRPDPARWQEQLKNVYAQLRDRYLEYPGVSRAALAVVPTNLETLRVGEGLLAIALASGAGPRTAAWAVDTLSLYVAGYALEASMVQQRRKDPGAIWVLSRDDLVSRFEALPEDKFPHTRRYATELTSGTGHDRFDFALDMVIDNLARAGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7P4</accession>
    <name>U5W7P4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561364.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33845"/>
    <dbReference type="EnsemblBacteria" id="AGZ45027">
        <property type="protein sequence ID" value="AGZ45027"/>
        <property type="gene ID" value="AFR_33845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6851"/>
    <dbReference type="HOGENOM" id="CLU_156547_0_0_11"/>
    <dbReference type="OrthoDB" id="2057744at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="10164" checksum="A05FEBBDDD61676A" modified="2014-01-22" version="1">MRPDACTLPTAEVPMRLAEFDALLATARLRDRTGSTRLRLVLDAPIETVRDLAARESECCSFFDFGVTPLGDVVEVTVTVPPAYVGVLDALAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7S4</accession>
    <name>U5W7S4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35010"/>
    <dbReference type="EnsemblBacteria" id="AGZ45258">
        <property type="protein sequence ID" value="AGZ45258"/>
        <property type="gene ID" value="AFR_35010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7084"/>
    <dbReference type="eggNOG" id="ENOG5033JQ7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153805_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="14281" checksum="48B50F5DEC54264F" modified="2014-01-22" version="1">MCRYAETTYKVHYVCVPCRRSSKLPWDDLDHPCPRCRKPMIPAGHDFAAPRHRDTSAWIAVAAVLAEGLRYDGFETCGCARQPKFRPRTSPQIRQRRRLATRRGLPFATLLTTRDPYDTPPPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7V8</accession>
    <name>U5W7V8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560382.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28920"/>
    <dbReference type="EnsemblBacteria" id="AGZ44045">
        <property type="protein sequence ID" value="AGZ44045"/>
        <property type="gene ID" value="AFR_28920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5862"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3899">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_4_1_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="838"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="910" mass="96698" checksum="9E09344175B2E0BF" modified="2014-01-22" version="1">MELFGRRAELAVLDGLLARAAHGSGSGLVLWGEPGIGKSALIEYAVAAASNAVVLRCRGTRMESELPFAALHELLWPVVDRLETLAAPQAAALRGALGMSRDQAGRFLVGAAVLSLVSGLARDRPVLVVVDDAQWVDEASAQCLGFLARRVAAEPVVVLLTEHEDPASGPWETLPALAVTGVAEDDARKLVAAAVPGADEALVDRTVRAAGGNPLALQELATLEHGSDDHAPPEVGPRLRRAFRDRAAAMTPPARALLLLATAEDRGDRHLVQRAGAQWGAGSSTWDEVLRSGLIRATGDRLEFRHPLIRAAVYDGASFPDRQAAHRALAAALPGDAVAERAWHLSAAADGPDEEVAALLEEAAEQCLRRSAGPVAVRTLRRAAELSPTSADAGRRLAAAARAAWDGGRAGAARQLLDEAEGLCGERAVVRLSGGLRGLLEFADGIPERAHRYLVYDMAEVPDAVRAIELGTVAVRAAWSAGRPDLQQEALEQVRAVRTDGEDALAELVPVLRNWWSCYDESGEITPASLDLDSRTVGRLGTAAWTLLPPVPLVQAWGIEGGLLDVLRVQAAQLRRRHELAALAMVLAQTAVLDLAAGRWAAAEASAAEGLQLAGEAGADHVAAQCHSCLGLLAAARGDSRAVAEHTTQILEVSVPRGVRALSASAYWHRGRAALFDGRPQEALSDLRCLADPVHEAAHPTFALFAAVDTVEAAVQAGDTGLVEAQVDRMERWAKRTDAPWVRTASHRLRALAHGRTPAAEDSFRAALDGVGADPFEYARLRLLYGEWLRRARRRADARYQLAEAAEIFDRLGAVALRSRALREHALTERPASSRAPGPAAAAGLTAQELRVAALAADRLTNREIAEQLLISPRTVGHHLASVYPKLGITSRAELARIDFAAGLRLRPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W898</accession>
    <name>U5W898_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34880"/>
    <dbReference type="EnsemblBacteria" id="AGZ45232">
        <property type="protein sequence ID" value="AGZ45232"/>
        <property type="gene ID" value="AFR_34880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7058"/>
    <dbReference type="eggNOG" id="COG4315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053665_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005297">
        <property type="entry name" value="Lipoprotein_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03640">
        <property type="entry name" value="Lipoprotein_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665799" evidence="2">
        <location>
            <begin position="20"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="23919" checksum="094BC0A1BD26AD45" modified="2014-01-22" version="1">MVVGAAIAAAFAVTGCAPAGLDNASDYGAQPAANAVDATATPTPGAEAEGDDAAEDDAADTDAPELSADEVTSELTAKEVKRMGETVQNQDGFVLYRFDKDKVKPEVVSNCKGDCAKVWPPAVVNKGEKPKLKGVDADLVGTVEREDGTLQLTIDKWPVYTYIGDKKPGQWKGQNVGTTWFVVTPEGKKNLTCLPAPSKAVAPPADDEGSDSDESGGDAGSDDSAGSDYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8E0</accession>
    <name>U5W8E0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_35080</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561608.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35080"/>
    <dbReference type="EnsemblBacteria" id="AGZ45272">
        <property type="protein sequence ID" value="AGZ45272"/>
        <property type="gene ID" value="AFR_35080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7098"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="23706" checksum="9D30F677C0938BD9" modified="2014-01-22" version="1">MTDPVIELRGVTRRYDEGPPALHDVSLTVAAGESVAVLGPSGSGKSTLLNLVAGLDRPSEGTVTVAGTRVETLGEAASARFRRAHVGLVFQFFNLLDDLTVLENVLLPAHLAGAPKRGARENATRLLDSLGIARHADAYPGRLSGGERQRVAVARSLINRPALLLADEPTGALDTASGEEVRRLLTDLHAGGQTIVLVTHDLRLAQACATRTIGMRDGRVESDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8T2</accession>
    <name>U5W8T2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional uridylyltransferase/uridylyl-removing enzyme</fullName>
            <shortName evidence="2">UTase/UR</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Bifunctional [protein-PII] modification enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Bifunctional nitrogen sensor protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="2">[Protein-PII] uridylyltransferase</fullName>
                <shortName evidence="2">PII uridylyltransferase</shortName>
                <shortName evidence="2">UTase</shortName>
                <ecNumber evidence="2">2.7.7.59</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="2">[Protein-PII]-UMP uridylyl-removing enzyme</fullName>
                <shortName evidence="2">UR</shortName>
                <ecNumber evidence="2">3.1.4.-</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="2" type="primary">glnD</name>
        <name evidence="5" type="ORF">AFR_36415</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[protein-PII]-L-tyrosine + UTP = [protein-PII]-uridylyl-L-tyrosine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13673"/>
            <dbReference type="Rhea" id="RHEA-COMP:12147"/>
            <dbReference type="Rhea" id="RHEA-COMP:12148"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:90602"/>
            <dbReference type="EC" id="2.7.7.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[protein-PII]-uridylyl-L-tyrosine + H2O = [protein-PII]-L-tyrosine + H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:48600"/>
            <dbReference type="Rhea" id="RHEA-COMP:12147"/>
            <dbReference type="Rhea" id="RHEA-COMP:12148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:90602"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.</text>
    </comment>
    <comment type="domain">
        <text evidence="2">Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GlnD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.59" evidence="2"/>
    <dbReference type="EC" id="3.1.4.-" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36415"/>
    <dbReference type="EnsemblBacteria" id="AGZ45539">
        <property type="protein sequence ID" value="AGZ45539"/>
        <property type="gene ID" value="AFR_36415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7369"/>
    <dbReference type="eggNOG" id="COG2844">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012833_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008773">
        <property type="term" value="F:[protein-PII] uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00277">
        <property type="entry name" value="PII_uridylyl_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010043">
        <property type="entry name" value="UTase/UR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47320">
        <property type="entry name" value="PTHR47320"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2 5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HD" evidence="4">
        <location>
            <begin position="413"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="584"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="686"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Uridylyltransferase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="751" mass="79422" checksum="FD9261B93A60409A" modified="2014-01-22" version="1">MVSEPSAPPKVPSGGSLDTLRDHLGAAARVDRAAALDAWLQTLFPAHLTGVSLVAVGGLGRRDCAPYSDLDLVLLHNGTAGIDRIASALWYPIWDARLGLDHSVRTLPEALSVAHDDVKVSLGLLDARHVAGDAALSAELIAATSDQWRRTAVRLLPQLKEITASRWAAHGELAFLLEGDLKEAAGGLRDLTILRGIGRAGVADTMRPAVRAANLRLLDTRDALHLAVGRRVDRLVAQERAAVAELLGLDDGDALLRRVAGDARTIAHALDDAWRAADRLRSGRRRGTPAGAPARRPVARDVVEQDGELVLARTAIGPVPDPTLSLRVAAAAATVRLPIARATCEWLAAFCPPLPMPWPPAARAALVSLLGSGQGLLPTWEACDRYGLIDAWLPEWARLRSLPQHHPIHRFTLDRHLVQAAYEATAFTREVDRPDLLLIGAFLHDVGKGLPGDHSIVGAPIAADIATRIGLPPADVATIEKLVRLHLLLPDVATRRDLSDPVTITTVAEAVGDPATLDLLHALTRADSHATGPAAWSDWKGRLIAELVRRVHTALDTGALPTPPEPDPELLEGELPAVHLDGDRVAVAAADRRGLLGSVAACLAMHRLDVIAADASTVDGRAIVEFTTQPRYGSPADPVALAADLRRVAAGDVSVTQRLRARAMSARGGAAPRVVWHRGAATDAAVLELRAADSAGLLFRVASALDDAGAEVRAARISTLGGDVVDAFYLVGAWAEAADRDRVEAAVIAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W905</accession>
    <name>U5W905_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562004.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37070"/>
    <dbReference type="EnsemblBacteria" id="AGZ45668">
        <property type="protein sequence ID" value="AGZ45668"/>
        <property type="gene ID" value="AFR_37070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7500"/>
    <dbReference type="eggNOG" id="ENOG5031QNW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117155_0_0_11"/>
    <dbReference type="OrthoDB" id="1918333at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045629">
        <property type="entry name" value="DUF6232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19744">
        <property type="entry name" value="DUF6232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="16823" checksum="AA25619066B922C3" modified="2014-01-22" version="1">MITYYRDPDVLVTSTGVRMGGRAYRLGDFAQVWHTRGRRSWSAVAGRGALGIAMITPIVIGAVGILIALVIDASTAMTIALAGGGVLIGLAAAPLADVLLEFVDRSYDRGSRRLEIWARVRGTDVLLLTTDNAQRFGQIYRALQRALDHDSVPRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W919</accession>
    <name>U5W919_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endo/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36815"/>
    <dbReference type="EnsemblBacteria" id="AGZ45619">
        <property type="protein sequence ID" value="AGZ45619"/>
        <property type="gene ID" value="AFR_36815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7448"/>
    <dbReference type="eggNOG" id="COG3021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052333_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="112"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="332" mass="35177" checksum="841B55B5D96EBFEC" modified="2014-01-22" version="1">MTITSSAPTTKPVRVRRVVWLVLLWLLVLPGTVWAVLRLGGWEQGALVQLFAFTPYVAAWSVMPVLAALVTRRWLAAAVATVTVALFAVMVLPRAFPDNNRGPQGGVALHVMTSNMLFGNADPAELVRLVRENDVAVLALQEFSPNAQTQLAAAGLGELLPYSSLGAEYGAGGSGLYSRFPITEPGVRQNGGGFRQAYGTIQPPGASAVVVESAHPLAPYSIEILNQWRNDLGEEPRADPKGVPRILLGDFNSTLDHAPLRDLISSGYRDAADAAGQGLIGTWGPYDGDPLPPVTIDHVLADKRLGVGAVSVHTIPGSDHRAIVAGLRLPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W961</accession>
    <name>U5W961_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_37075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562005.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37075"/>
    <dbReference type="EnsemblBacteria" id="AGZ45669">
        <property type="protein sequence ID" value="AGZ45669"/>
        <property type="gene ID" value="AFR_37075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7501"/>
    <dbReference type="eggNOG" id="ENOG5031YQY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2423108_0_0_11"/>
    <dbReference type="OrthoDB" id="2020860at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="91" mass="9470" checksum="1CA012D8291AA3D7" modified="2014-01-22" version="1">MTAPGEHQDLIRRWLAGEVVGNQVGIKIVGGPFDGRTKIVGLDQNQKPPPGFRASGGPVGPIRHLYEVAPSSSAASGWIYAHIGADPDTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W970</accession>
    <name>U5W970_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative cation/multidrug efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562083.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37470"/>
    <dbReference type="EnsemblBacteria" id="AGZ45748">
        <property type="protein sequence ID" value="AGZ45748"/>
        <property type="gene ID" value="AFR_37470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7579"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_11"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="427"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="530"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="855"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="881"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="906"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="956"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="986"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1111" mass="114501" checksum="D90CE16E7BC479B5" modified="2014-01-22" version="1">MSFLTRLSLANRGLVALIAVVITGFGIYTVPSLKQQLLPSLEFPGAFIGVVLPGASPEIMEEQVTKPIEDAVKGADGLDTITSTTREGSATIQVLFEFGTDLDNAVNQLTTSVNRIQPTLPDDVEPTIFAGSTDDIPAIILAASDGKNDQAALANRLQQTVVPELNAIEGVREVAVTGTRDQQVVIVPDRAKLGAAGVDPRSIATLLQNNGISIPAGAVTDGSRSLSVQVGTPIATVDQLKGLYLNGSKGPVRLDSVASVTQQLAAATSFTRTDGVESLGIAVTASPDGNPVNISHEVRDKLAELESASGSTLTVITDQAPFVERSIESLTTEGLLGLIMAVIVILVFLLSVRSTLVTAVSIPLSVVIALIALKLGDYSLNLLTLGALTIAVGRVVDDSIVVLENIKRHLGYGEAKTHAITAGVKEVAGAVTASTLTTVAVFAPIALVGGFVGQLFAPFAITVTVALIASLFVSLTVVPVLAYWFLSPAAGGSESEEVRKAAEEKELRSPLQRAYLPIIRWVTRSKGTRWSTVAIGVVVLIGTLGLASRLETNFLDESGQDTITVSQDLPVGTDLAATNEAAKKVEAILATRGDVKNYQVTVGNGDFNPFVGSGGASGASFNVALKDDVDAAEVSDELREQFKGMTDVGEVTIGSDSGTGFDGSALAVVVQAADQTALESATKQVQDAMAGTKDVTDVSSSLAASVPRLDVKVDRAAAAARGLTESAIGQSVAGAFRAAPAGQISIDGVSQSVVVTLGAPPKDAAALRNLPLTAPNGQVVTLDSVADVSEVDGPEEVTRIDGNRSVTVNGTATGSNLSKTTADLTARLDDLQLPPGASYEIGGVSQDQADAFADLGLAVLAAIAIVFVIMVATFRSLLQPLLLLVSIPFAATGAIALLLATGTPLGVPALIGVLMLVGIVVTNAIVLMDLINHYRQAGLGVQEAVIEGGRHRLRPILMTAIATIFALLPMALGLTGEGGFISQPLAIVVIGGLVSSTLLTLVLVPTLYTMAENTKLKARTRRERKRALKAVESSGAGGDAPKHAADEPEVVVPADDPTPASSGGAHEAPEGEPAGSPARSSADPQSGALRGYTDQFEVLKMPRRPANPPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W981</accession>
    <name>U5W981_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TM helix repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560868.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31375"/>
    <dbReference type="EnsemblBacteria" id="AGZ44535">
        <property type="protein sequence ID" value="AGZ44535"/>
        <property type="gene ID" value="AFR_31375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6350"/>
    <dbReference type="eggNOG" id="COG0668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049564_0_0_11"/>
    <dbReference type="OrthoDB" id="1896788at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008910">
        <property type="entry name" value="TM_helix"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05552">
        <property type="entry name" value="TM_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="447" mass="47676" checksum="A8A851AA5F319FA5" modified="2014-01-22" version="1">MTGADLSDALSDMWRSVLLFVPAALAFIAILVIGYVLARLVRTVVGKTLRRVGFDRAIERGPVGRALGGRVEPSDLCAKIAFYAVLLFALQLAFGIWGPNAVSDLLTALVAWLPRAFVAIVIIVVAAGIGGAVRDLIGSALGGLAYGRVLAQAAYWLIVGLGIIAALEQVQIATAVTQPVLIAVLATVAGVLIVGVGGGLIRPMQQRWELWLVRASAESAAIREHARSYNEERARLAAELKAAEEKAAAEKKAAEERAAAEKKAAEEKAAAEKRAAEEKAAAERKAAEEQAAARAAEEARLAEQARIAEAERVARETALAEARRIEADQAAEAQRQAAEAQRQAEEAERQAAEAQRAVEAEHEAAAEKTQVIPVQRRGDEDDRPHPVPGFGGPDPADTTTRLPVGLAGDDSDTTETISQSEHRRVLPQPDEAADDERTQVIRRPKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9I5</accession>
    <name>U5W9I5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydroxydechloroatrazine ethylaminohydrolase</fullName>
            <ecNumber evidence="2">3.5.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.99.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560899.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31530"/>
    <dbReference type="EnsemblBacteria" id="AGZ44566">
        <property type="protein sequence ID" value="AGZ44566"/>
        <property type="gene ID" value="AFR_31530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6382"/>
    <dbReference type="eggNOG" id="COG0402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_3_11"/>
    <dbReference type="OrthoDB" id="1592010at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="56"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="450" mass="47575" checksum="8015AA4E2C32C1B3" modified="2014-01-22" version="1">MIVIENVAVATVDANGTEYTDGHVVVGDDGRIRAVGSGHAGRFPGSHRHVDGTGCLVTPGLVNTHHHLYQWVTRGLAQDDNLFGWLTTLYPIWGRIDAETVGAAAGAGLGWLALSGCTTSMDHHYVFPRDGGDVLAAEIEAAQQIGLRFHPTRGSMDLGQSSGGLPPDHVVEETDAALAATEAAIDRWHDPSPESMLQIGVSPCSPFSVTSRLMSEAAELARRKGVRLHTHLAETDDEEDFCREKFGCTPVEYAERLGWLGEDVWLAHGVHLDDAAVAKLAATGTGVAHCPSSNARLGAGLARVRDLLDAGAPVGLGVDGSASQEAAHLGEELRQALYVARLRGGPKAMSARESLRLGTMGGARCLGRQDDIGSLEVGKLADLVLWRLDGLGHDGIDDKVAALVLGPAAPVELALVGGRPVVERGQLVNADAEALTRRARRAHRRLLSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9M9</accession>
    <name>U5W9M9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">ATP synthase subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP synthase F0 sector subunit a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">F-ATPase subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">atpB</name>
        <name evidence="13" type="ORF">AFR_37365</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11 12">Cell membrane</location>
            <topology evidence="11 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562062.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37365"/>
    <dbReference type="EnsemblBacteria" id="AGZ45727">
        <property type="protein sequence ID" value="AGZ45727"/>
        <property type="gene ID" value="AFR_37365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7559"/>
    <dbReference type="eggNOG" id="COG0356">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041018_0_1_11"/>
    <dbReference type="OrthoDB" id="867266at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01393">
        <property type="entry name" value="ATP_synth_a_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023011">
        <property type="entry name" value="ATP_synth_F0_asu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045083">
        <property type="entry name" value="ATP_synth_F0_asu_bact/mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410">
        <property type="entry name" value="PTHR11410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="SSF81336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01131">
        <property type="entry name" value="ATP_synt_6_or_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00449">
        <property type="entry name" value="ATPASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11 12" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 11 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 11 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ45727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="29761" checksum="097B19CF9774B566" modified="2014-01-22" version="1">MIGQSTVLAAEFPPSVEDFYLPSILPWGAHDSYWFTKITLLVWVAVALIIIYFLVSYRKPQLVPTKKQWLAESLYGFVRNNISVEMIGPRGVAFAPYLTTLFCFILVMNFFSIVPLIQISPNSHIAFPAILAVISYVMFIYVGIRHHGSFFKYMKSALIPPAPWFILPLLIPIEFFSNFLVRPFSLAVRLFANMFAGHMLLLVFTLGGFAMISANVWFAPFSIVSWIMTIALTFLEFLVICLQAYVFTVLTASYVQGALADEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9R2</accession>
    <name>U5W9R2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_32285</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561047.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32285"/>
    <dbReference type="EnsemblBacteria" id="AGZ44715">
        <property type="protein sequence ID" value="AGZ44715"/>
        <property type="gene ID" value="AFR_32285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6535"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_1_0_11"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="354" mass="36067" checksum="6FA42B7720ECD200" modified="2014-01-22" version="1">MTTTAAERAGYTSADARTRARGGLTARGLGLVLALAGLIVVSVVSLAVGTKSIPLGTVWDALFSRGSTTEDVRIILDLRVPRTLIGLAAGVALGLAGALMQALTRNPLADPGILGINAGAATAVVLAIRVLGVGSLTGYIWFAFAGAAGAFVVVYLLGTRGRSGASPERLALAGAAVGAALLAVTNAVALLDVTTFDRYRLWTVGALSGRDLDVLWGVLPFIVIGTVLALGLGPSLNVIALGEDTARALGANLNRTRVLSAIAITLLCGAATAAVGPIAFVGLAVPHIARMLVGPDQRWLLPYSLVFAPTLLLIADIAGRVLARPGEVQAGLVTAFIGAPVFLAVILRRRIVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9W0</accession>
    <name>U5W9W0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37765"/>
    <dbReference type="EnsemblBacteria" id="AGZ45807">
        <property type="protein sequence ID" value="AGZ45807"/>
        <property type="gene ID" value="AFR_37765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7637"/>
    <dbReference type="eggNOG" id="ENOG5033QRR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_835861_0_0_11"/>
    <dbReference type="OrthoDB" id="1532395at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="34563" checksum="75D179391AA53B8F" modified="2014-01-22" version="1">MEDLVRAAQEKQANLTPDPQRVLAAFPRRHTQLVRRRRLTVAVVAAAAVTAVLAVPVASLTDRGAGPVPQPAIKPTVSVPLPPAHVALRYQPGWLPDGYTEFYRNNRLGGTSRVWTPDDLTVPLAKNRRFVFLNVDPLRSLDGENGEQVDIAGSRGVYVPEANQVTWLSGGYTLAVGTINLALPKSTLLRIARSLRADEEVFPIPFRTPELSAGLEVVGWTVDGAGPQNWVGTVELGKNPGEPSGQVSISVGTATSAPKGGSERLVGGRPARLVKTSTGTSYLVVTLGGGRLLTVRSSVLPEVQLSRLAEGVGSPAAGKLSWIGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAG4</accession>
    <name>U5WAG4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uma2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38790"/>
    <dbReference type="EnsemblBacteria" id="AGZ46012">
        <property type="protein sequence ID" value="AGZ46012"/>
        <property type="gene ID" value="AFR_38790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7849"/>
    <dbReference type="eggNOG" id="COG4636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076312_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06260">
        <property type="entry name" value="DUF820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012296">
        <property type="entry name" value="Nuclease_put_TT1808"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008538">
        <property type="entry name" value="Uma2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05685">
        <property type="entry name" value="Uma2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uma2" evidence="1">
        <location>
            <begin position="21"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="172" mass="18618" checksum="09655F8E75DD88C6" modified="2014-01-22" version="1">MTSALIGRSLPMEALATTEAEYLQLGETSARTELWDGGVLTCPRDTPRHQLILNALANALRANRTDLNVISGVPVRLGPGRIAVPDLIIAGTIDLDQPLVEASSVRLVCEITSPASMTVDWTLKMHAYAQARIPCYLVAEPERGLLHSNMLSAQGYVEQSVMQLVRVTGLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAN3</accession>
    <name>U5WAN3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Tryptophan 2,3-dioxygenase (Vermilion)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38965</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562381.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38965"/>
    <dbReference type="EnsemblBacteria" id="AGZ46047">
        <property type="protein sequence ID" value="AGZ46047"/>
        <property type="gene ID" value="AFR_38965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7886"/>
    <dbReference type="eggNOG" id="COG3483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063240_0_0_11"/>
    <dbReference type="OrthoDB" id="1660023at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004833">
        <property type="term" value="F:tryptophan 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004981">
        <property type="entry name" value="Trp_2_3_dOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138">
        <property type="entry name" value="PTHR10138"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03301">
        <property type="entry name" value="Trp_dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="SSF140959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="26680" checksum="BDA01B1F1CC5B9D8" modified="2014-01-22" version="1">MRKLQRDETYRDLLRLDELLDSTLVRDGGPDRMLFLVTHQICEMWFALILGHLDEARSAILAGDPGTATARIGRLPAIMQVLSAQFDVLATLTPAAFERIRADLGSASGIQSSQWREIEFACGLRETRYLTVPGFTEQETARLVRRMDEISVADAYAQLAKQRRDAPDPGAMVAVEQLRAAMLDFDEAVVAWRARHASLAERFLGGRPGTAGSEGSSYLWRMTARRLFPDAWPGANGGTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAR9</accession>
    <name>U5WAR9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562650.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40320"/>
    <dbReference type="EnsemblBacteria" id="AGZ46318">
        <property type="protein sequence ID" value="AGZ46318"/>
        <property type="gene ID" value="AFR_40320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8160"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_11"/>
    <dbReference type="OrthoDB" id="1261526at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="252" mass="26101" checksum="542778EC962CFD1A" modified="2014-01-22" version="1">MVQKKIAVVTGASSGIGAATARRLATEGFHVVAAARRQDRLEQLVAEIGPDATAVVCDVTSDESVAALATTVTGLGAPVALLVNNAGGARGLDPVSDGSVADWQWMYDVNVLGTLRTTQALLPALEASGAGTVVTVGSTAAFTVYEGGAGYTAAKHAQNALVGTLRLELAGRPVRVIEIDPGMVRTEEFSLKRLGDQGKADAIYAGVKEPLVADDIADCVAWVATRPQHVNIDRLVVRPIAQAAQHKVARES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAT3</accession>
    <name>U5WAT3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562665.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40395"/>
    <dbReference type="EnsemblBacteria" id="AGZ46333">
        <property type="protein sequence ID" value="AGZ46333"/>
        <property type="gene ID" value="AFR_40395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8176"/>
    <dbReference type="eggNOG" id="ENOG5031TG8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130806_0_0_11"/>
    <dbReference type="OrthoDB" id="1624759at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007809">
        <property type="entry name" value="FlgN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036679">
        <property type="entry name" value="FlgN-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05130">
        <property type="entry name" value="FlgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140566">
        <property type="entry name" value="SSF140566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17572" checksum="FEF02C0F77CB8AD8" modified="2014-01-22" version="1">MSLTDLSSVLWRARELLELLLFKLEEEQLLLASNRSRWLAHATREVEVVLDQIRQTEVARAAYAQAVAIELGLPAEASLGQLADAAPAPWSELLHQHRKAFLTLTSEIGAVAEANRDLLTAGQRAARETMLAFAGTVETYSPQGKTVSGGVRRPSLVDEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB44</accession>
    <name>U5WB44_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45156">
        <property type="protein sequence ID" value="AGZ45156"/>
        <property type="gene ID" value="AFR_34490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6978"/>
    <dbReference type="HOGENOM" id="CLU_2021760_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="12984" checksum="75CE30B956D1F4D4" modified="2014-01-22" version="1">MDYVICQYPKRNSTDPGVEINVRAFRGQNGTAQATTLADVDQGSTSDSYADFVTVPGSGDNGGFAWSDDKELGLATHAGNAYVVLVVFPSDEANKNADSRPALQKQVPGLTKIMADMLTALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBE8</accession>
    <name>U5WBE8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_40290</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40290"/>
    <dbReference type="EnsemblBacteria" id="AGZ46312">
        <property type="protein sequence ID" value="AGZ46312"/>
        <property type="gene ID" value="AFR_40290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8154"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_60_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="15"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="411"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="467" mass="49094" checksum="DB0DA4BEA5B32AF3" modified="2014-01-22" version="1">MRRWLQQATGGLPRQFWFLWTGTLINRLGSFVIIYLAIYLTQQLDFSQSQAGLVLGAYGVGGAVGTMTGGVLADRWGRRPTLLTAQFGAAALMLGLGFAQGFWQLVAGALLLGLFAEGVRPAFQAMMIDIVPDRDRLRAYSLNYWAINLGFASAAVLAGFAAQFDYLLLFVVDAGTTLITALISLIFLAETRPSGTRGVPRTKGAPGLGTVFRDRVFLIFLGLNFFVVLIIMQHMSTLPIAMSADGLGPATFGWVIAVNGIMIVAGQLFVPKLIGGHDRSRVLALATLIIGVGFGLNAFAGAAALYVLSVVIWTLGEMLQTPSNSALVAELSPAALRGRYQGVNSLSWSAGSALAPIVGGFVQQHLGSTALWLGCAAIGVVVAAGQIFSGPSRERRVTALRAALPATDRLAEPEIAAAGPEAEPVAAPGTRPATTAEEPAVSPAATEQAHPEDVTPPEITLKVPARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBF4</accession>
    <name>U5WBF4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="11">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">cysS</name>
        <name evidence="14" type="ORF">AFR_41100</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562806.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41100"/>
    <dbReference type="EnsemblBacteria" id="AGZ46474">
        <property type="protein sequence ID" value="AGZ46474"/>
        <property type="gene ID" value="AFR_41100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8319"/>
    <dbReference type="eggNOG" id="COG0215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_1_11"/>
    <dbReference type="OrthoDB" id="952207at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="PTHR10890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DALR_2" evidence="13">
        <location>
            <begin position="346"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="270"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ46474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="471" mass="51875" checksum="2A0B9E296FE8855E" modified="2014-01-22" version="1">MTLRLHDTATRSVRDFVPMTPGQVGVYLCGLTVQSIPHIGHLRSAVNYDVLRRWLLHIGLEVNFVRNITDVDDKILVKSQETGRPFWAIAYANKLVLDADYRSLNVLPPTYEPLATGHITEMHELIRELIERGNAYESAEGNGDVYFDVPSFKDYGSLSGQRPEDMLSAGEAPERGKRHPADFALWKGVKADEPGDASWASPWGRGRPGWHIECSAMARRYLGSEFDIHGGGLDLTFPHHENEIAQSRSAGLPFARYWVHHALLNLGEAKMSKSLGNVIDVQAVLEMGIRPVELRYYLGVPHYRSRIDYSDDALREAATAYRRIEGFVQRAVEVVGSGRPKDVPPAFAAAMNDDLNTSAALAVVHDTIREGNTALAASDETGVRAALTEVRAMLGVLGLDPLDPAWHDGESGGDLKPIVDSLVALALEQRAQARSRKDWAAADSVRDQLKNAGVQVEDTPAGPRWTVGEQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBF9</accession>
    <name>U5WBF9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_35125</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(R)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561617.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35125"/>
    <dbReference type="EnsemblBacteria" id="AGZ45281">
        <property type="protein sequence ID" value="AGZ45281"/>
        <property type="gene ID" value="AFR_35125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7107"/>
    <dbReference type="eggNOG" id="COG0229">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_8_5_11"/>
    <dbReference type="OrthoDB" id="1650261at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081">
        <property type="entry name" value="PTHR46081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="SSF51316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00357">
        <property type="entry name" value="TIGR00357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MsrB" evidence="4">
        <location>
            <begin position="12"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="15042" checksum="84687BCC347B09D6" modified="2014-01-22" version="1">MATDKTTLPTTEEEWRVRLSPDEFRVLRQGATEPAWRGEYVDTKTEGTYECRACGAALYPSDTKFDSHCGWPSFDDAIPGAIKEIEDRSLGMVRTEIQCARCGSHLGHVFKGEMMTAKNTRHCVNSLSIKLDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBT4</accession>
    <name>U5WBT4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35825"/>
    <dbReference type="EnsemblBacteria" id="AGZ45421">
        <property type="protein sequence ID" value="AGZ45421"/>
        <property type="gene ID" value="AFR_35825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7251"/>
    <dbReference type="eggNOG" id="ENOG5032V8W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108795_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019660">
        <property type="entry name" value="Put_sensory_transdc_reg_YbjN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10722">
        <property type="entry name" value="YbjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="15340" checksum="AA5371FE33E22392" modified="2014-01-22" version="1">MSVLPQPLSHEMIVDALNAREFAHFVDDDGDIGGNWQGCLIYFFRLGKEQEVLQVRSLTRTVFSTDDVPRLYAFCNAWNHDRLWPKAYVHTADDGTVRVVGEVVGDWERGVTPEQLDQVMICGIATGCELADAVAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBU9</accession>
    <name>U5WBU9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">GatB/YqeY domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562920.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41675"/>
    <dbReference type="EnsemblBacteria" id="AGZ46589">
        <property type="protein sequence ID" value="AGZ46589"/>
        <property type="gene ID" value="AFR_41675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8434"/>
    <dbReference type="eggNOG" id="COG1610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079430_2_0_11"/>
    <dbReference type="OrthoDB" id="1498741at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016884">
        <property type="term" value="F:carbon-nitrogen ligase activity, with glutamine as amido-N-donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019004">
        <property type="entry name" value="YqeY/Aim41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042184">
        <property type="entry name" value="YqeY/Aim41_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055">
        <property type="entry name" value="PTHR28055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09424">
        <property type="entry name" value="YqeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="150" mass="15675" checksum="B45CC9DDB885D28F" modified="2014-01-22" version="1">MGTLKDRLNDDLHSAMKGRDELTTSTLRMALTAVRNAETSGTAAKVLTDEEVLAVLTKEAKKRREAANAFAGAGRTDQAAKENAEGEILDRYLPAQLSDAEIDGLVADALQAGGFTSKCQMGPAMKAAQAAVAGRAEGGRVAASVRKQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBX0</accession>
    <name>U5WBX0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FhuF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42195"/>
    <dbReference type="EnsemblBacteria" id="AGZ46693">
        <property type="protein sequence ID" value="AGZ46693"/>
        <property type="gene ID" value="AFR_42195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8540"/>
    <dbReference type="eggNOG" id="COG4114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1068804_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022770">
        <property type="entry name" value="FhuF_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06276">
        <property type="entry name" value="FhuF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FhuF" evidence="1">
        <location>
            <begin position="83"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="28281" checksum="EF073B2F98A0C6BB" modified="2014-01-22" version="1">MTAALDRALASDYAQQPLAPVTATLRAMFGTSTELPGLAPDLLVQDPTGWLPTSDLAGASLDTLLDSSRQRWQAQPHAAAALAWKAYTYWLTLPAVLGWASARRVPLLTGTDVLMHFSDPRPLVTIGLRHDITVAVLPSDPLAVSGLPQVRVVADEAALLEELRRSLLDEHLTPLLDAIHTKVRLGSRTLLGSLASGVAYAVLRSADVLPGSSAESIAALLGTLGVDDLIELVPGRNGKLDVQRKTCCLAFTLPKPKVCMGCCIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC53</accession>
    <name>U5WC53_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative molybdopterin biosynthesis protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563129.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42720"/>
    <dbReference type="EnsemblBacteria" id="AGZ46798">
        <property type="protein sequence ID" value="AGZ46798"/>
        <property type="gene ID" value="AFR_42720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8646"/>
    <dbReference type="eggNOG" id="COG0314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089568_1_1_11"/>
    <dbReference type="OrthoDB" id="1877633at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="SSF54690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="15141" checksum="1B0D568E78D7BF95" modified="2014-01-22" version="1">MTTIESVRIADIRDATLSLAAHEEAVSDPRAGAVVSFQGVVRDHDGGRGVTLLEYEGHPTAAAILREVADEIAADPDVYAVAVSHRVGVLQIGDVALVASVSTAHRAAAFAACARLVDEAKARLPIWKRQVFADGTEEWVNCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC55</accession>
    <name>U5WC55_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561901.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36545"/>
    <dbReference type="EnsemblBacteria" id="AGZ45565">
        <property type="protein sequence ID" value="AGZ45565"/>
        <property type="gene ID" value="AFR_36545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7393"/>
    <dbReference type="eggNOG" id="COG0711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078484_2_1_11"/>
    <dbReference type="OrthoDB" id="1602369at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="18759" checksum="C98D45D38477DA3F" modified="2014-01-22" version="1">MDPLDRIDEIISMVETARSVPMSRTNFMFDRGEMVAILDELRAELPSDLRRAVALLEERDKIIDAGKREADRIIGEGETEHARLVSVNEITVSAEHEGARIIAEARAEAQRLREEVDDYVDTALANFEQFLTRALGSIERGRDKMHALREIGTFQGEDHERPLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE45</accession>
    <name>U5WE45_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proline dehydrogenase</fullName>
            <ecNumber evidence="3">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_40230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + L-proline = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562632.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40230"/>
    <dbReference type="EnsemblBacteria" id="AGZ46300">
        <property type="protein sequence ID" value="AGZ46300"/>
        <property type="gene ID" value="AFR_40230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8142"/>
    <dbReference type="eggNOG" id="COG0506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061158_0_1_11"/>
    <dbReference type="OrthoDB" id="1746057at2"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00373"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:proline catabolic process to glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008219">
        <property type="entry name" value="PRODH_bac_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PTHR13914"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000196">
        <property type="entry name" value="Pro_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pro_dh" evidence="8">
        <location>
            <begin position="42"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="224"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000196-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000196-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="33526" checksum="27CA2614CDDF89F5" modified="2014-01-22" version="1">MLRSVFLAAAGSSRMERLVSSAPVTKGVVRRFVAGEGVDDALRTSRELADDGLAISLDYLGEDTLTAEQATATRDQYLTLLGRLKSAGLTPAAEVSVKLSALGQKVDDRMAYDYAHRICTAAAEAGTTVTLDAEDHTTTDSTLEILAELRKDHPGTGAVLQAYLRRTEGDCRELAAAGSRVRLCKGAYAEPESVAYQSALDVDKAYVRCLNILMSGDGYPMIATHDPRLIAIAEDRARWFDRSADEFEFQMLYGVRPEEQTRLAAAGHTVRVYVPYGDQWYGYLMRRLAERPANVAFFGRALASRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF82</accession>
    <name>U5WF82_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (Penicillin-binding protein 4)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41560"/>
    <dbReference type="MEROPS" id="S13.004"/>
    <dbReference type="EnsemblBacteria" id="AGZ46566">
        <property type="protein sequence ID" value="AGZ46566"/>
        <property type="gene ID" value="AFR_41560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8409"/>
    <dbReference type="eggNOG" id="COG2027">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017692_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000667">
        <property type="entry name" value="Peptidase_S13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30023">
        <property type="entry name" value="PTHR30023"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02113">
        <property type="entry name" value="Peptidase_S13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00922">
        <property type="entry name" value="DADACBPTASE3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00666">
        <property type="entry name" value="PBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="454" mass="45934" checksum="320CBC2408889B20" modified="2014-01-22" version="1">MLVVRPGPVEGWLAAEPTTAPTVAPTPDPTPTPVLAAVDTTGTAPTPEGVKDAIGPLVKAPALGNQVNVSVVDTATSESLFEQNADTMTTPASTTKLLTAATVLAARGPAYRLSTRVVAGPNPGEVVLIGGGDPTLSVNAKGQFPGAARLDKLADQVSKALAGQKPTKVIIDTSLYTGPLTAKGWDSDVISGGQVSRIQSLMTNAGRIKPVHNDFGGDPRFNDPAMAAGRAFAKELGLPSGAVAKGKAPAAAETPASTGPSTAPGTELGKVDSPPLVHVVDWMLEQSDNVIAEAMARQVALAAGQEASFEGAAAAMVAKLGELELPGDEANLYDASGLSRHNGISPKLLTDVLTLAASGKHPELSAMFGGLPVAGWSGTLRTRFATPSPNKVGQGLVRAKTGSLTGVNTISGQVVTKDGRLLVFAIMADATGESGAARQALDKIAAKLVTCGCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFH6</accession>
    <name>U5WFH6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563002.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42085"/>
    <dbReference type="EnsemblBacteria" id="AGZ46671">
        <property type="protein sequence ID" value="AGZ46671"/>
        <property type="gene ID" value="AFR_42085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8516"/>
    <dbReference type="HOGENOM" id="CLU_2930745_0_0_11"/>
    <dbReference type="OrthoDB" id="2098925at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="62" mass="6439" checksum="1E72E0584F561226" modified="2014-01-22" version="1">MTGAVALAALIGLASLLAGVALGWYLRRTNDWCSHCGDQLSCEGCGSKAAWPKTRETESLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VN33</accession>
    <name>U5VN33_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polycystic kidney disease protein 1-like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00495"/>
    <dbReference type="EnsemblBacteria" id="AGZ38388">
        <property type="protein sequence ID" value="AGZ38388"/>
        <property type="gene ID" value="AFR_00495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.101"/>
    <dbReference type="HOGENOM" id="CLU_486304_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="560" mass="55646" checksum="9EAE09066211A91C" modified="2014-01-22" version="1">MPEQPDDDGLVPPIKVMRKRLNGVYSAERGFAGPTRRYVLIVAMLVGLASLPTLAAITAGSNELDDGRTGAMDVPFIPPASTGPVQVRPTASAGTGTPSPDASEPVQGQAQKRKTKPKGYAQSNRGAESPDPSRDRGDDSGSASGNGNGNGSGRGNGTGSGSGSGSNSGSGTGSASGAGDGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGASPERPAKPAPGLPSIPSKPAVPDESQQIPDFPDEPDSPDDPPGHSPGDDSGSNGGDGHSGSDGDGSNDGGDGHSGSDGDGSNDGGDGHNGSDGDGSNDGGDGHNGSDNGGSNDGGSDGGSGHGGSGHGGAGDGDSGQGGTGDGDSGHGGSGDGDGDGPVKPPAKPVRPPWCWDRTDCSARPSHHHRPDRSRDKDCDDSRTERDRSGKQHRRSKITEAPDREDAGSSPVPARKPVSIRSVKAARTDSSRRSEVTERPQNLRPARGGDRTHNSHRYQQSSSDRGGEGGPTATALRAYRGSHRAERMHRADDRTGAHHRSSRVGRHHADPQQQDHQNRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VN55</accession>
    <name>U5VN55_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357352.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00595"/>
    <dbReference type="EnsemblBacteria" id="AGZ38408">
        <property type="protein sequence ID" value="AGZ38408"/>
        <property type="gene ID" value="AFR_00595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.120"/>
    <dbReference type="eggNOG" id="COG0235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_3_4_11"/>
    <dbReference type="OrthoDB" id="599627at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="1">
        <location>
            <begin position="10"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="222" mass="23851" checksum="1F8F8115D857A7E9" modified="2014-01-22" version="1">MNYVPGDLRDQLAHVGYDVVQAGLVCGSGGNLSARMPDEDACWVTASGAWLDRLSRTTFAPVRISDGAPATIGTLPAPRLTPTSEIALHLALYRARPDVNAVIHLHPQTALLLDALGEHIRIVTTDHAFYLKRVSTVPFRLPGGTEVAALTAAMAADGTDCLVLSQHGCVVMADSVELAHKRARNLEEAALLTYRALTLGRLDNLREVPEEFLDRLPSAISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPV4</accession>
    <name>U5VPV4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357777.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02725"/>
    <dbReference type="EnsemblBacteria" id="AGZ38834">
        <property type="protein sequence ID" value="AGZ38834"/>
        <property type="gene ID" value="AFR_02725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.548"/>
    <dbReference type="eggNOG" id="ENOG502ZKC6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_358556_0_0_11"/>
    <dbReference type="OrthoDB" id="689428at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="513"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="543"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="575"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="756" mass="79784" checksum="B6E303BF2E8BFF18" modified="2014-01-22" version="1">MTTLLTRPAPAPAAASRPALRPWLAAVPWLLVAVVLAGALYRADVPGREIATYGLYFLAGVVPPGTLVHRAIRGSRGNLPEDLGYGAATGLFVQIVGWALAAATGLQALLIGWPVLVLVAFVAVPRLRQHWRIAEPEPLPLRWSWLICGALLGVIAWGAAAWSGTPLPPADAVYYQDLMYHLALVQEMTRAMPFEVPQLAGDTLRYHYLSDADMAAASMITGISPATVLLRLWMVPIGATAVLVVAALTRELSGKWWAGPLAGGVAFAGMPLTLGAPIDAYGGSSLSYASPSQTYAMPLLGLLVAIVVDLLRGRKLGPASFLAPPLALVCAGAKSSALPPLIAGLGLAALVVWWRDKRFPRAAAGLLLALTVVMLIGTRLFAGGGAGTLGVQPLSLLRWMEPYSKTLGTGDGVAPGGLIAPGLQGIGLTGGGFVAGVIGWWVLMQSPRLLGLLSLRRRSDPAAWLLGGMTVAGAGAAWALWHPSASQLYFFLCAAPFGVVLTVWMLAERTRHWWVPVAGLLAGALWSVVAPVVAAPARNTLGGWAWALAVPVLRTLGLVVIAAILVLALRRRFRVLATAVTAAVLGASLATGLAGFVRVLGQETGQPSTARMVTQEEMGAALWLDEHAADDDVVATNVHCTPMARYQPCDARAFWVAGLGGHRTLVESWAYSDETVAANGVGGLKYVLQPPPDQALYDLNQRAFTTADPADLTRLRQQYGVKWLFADTRAGEVSPDLARLAQLRHTSGPVTIYELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ62</accession>
    <name>U5VQ62_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phytanoyl-CoA dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357900.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03340"/>
    <dbReference type="EnsemblBacteria" id="AGZ38957">
        <property type="protein sequence ID" value="AGZ38957"/>
        <property type="gene ID" value="AFR_03340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.672"/>
    <dbReference type="eggNOG" id="COG5285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063392_0_0_11"/>
    <dbReference type="OrthoDB" id="1070946at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="40586" checksum="11E1C2E6BDC57961" modified="2014-01-22" version="1">MTSWFSAADCRLSDFRAVVERQTDRAGYPHAADVRQNVLIYDRTVLDGDRREVQAELARALMDGPGIVVFTGAFAADVVDRATGVFAALIDEQKASGAVAGDHFAKPGANDRIWGGQEKFALRDPEVFADYYDNATLALICEAWLGLGYQVTSQVNVVNPGGQAQVAHRDYHLGFMSREQAQSYPAHIHALSPVLTLQGAIAHIDMPVETGPTLYLPHSQKYPAGYIAFHQPEFTAYFEENHVQLPLAKGDAVFFNPALFHGAGTNRSADVKRMANLLQVSSAFGRAMESQDRAAMCRALYPVLQKFEGNVGNVIAASAEGYAFPTNLDRDQPIGGLAPQTQAELMHQALAEKWDPTQLSEALNAQYERRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQF9</accession>
    <name>U5VQF9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">50S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">rplJ</name>
        <name evidence="9" type="ORF">AFR_04445</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and the large rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358148.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04445"/>
    <dbReference type="EnsemblBacteria" id="AGZ39178">
        <property type="protein sequence ID" value="AGZ39178"/>
        <property type="gene ID" value="AFR_04445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.899"/>
    <dbReference type="eggNOG" id="COG0244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092227_1_0_11"/>
    <dbReference type="OrthoDB" id="1470978at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00362">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022973">
        <property type="entry name" value="Ribosomal_L10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002363">
        <property type="entry name" value="Ribosomal_L10_eubac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="PTHR11560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01109">
        <property type="entry name" value="RIBOSOMAL_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="181" mass="18784" checksum="8FED97564053224B" modified="2014-01-22" version="1">MADKPVRADKATAVAELTDSFRNSAATLLTEYRGLTVSEITELRRSLGNETKYSVSKNTLAKRAATDAGIEGLDALFTGPTALAFVGGDPVEAAKGLRAFAKAHPVLVIKGGVFEGKAISAEEVSKIADLESREVLLAKLAGAMKGNLTKAAATFQAPLAQVARLAAALQDKRAQDGSAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQY1</accession>
    <name>U5VQY1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Holo-[acyl-carrier-protein] synthase</fullName>
            <shortName evidence="8">Holo-ACP synthase</shortName>
            <ecNumber evidence="8">2.7.8.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">4'-phosphopantetheinyl transferase AcpS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acpS</name>
        <name evidence="10" type="ORF">AFR_04825</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="EC" id="2.7.8.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the P-Pant transferase superfamily. AcpS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358288.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04825"/>
    <dbReference type="EnsemblBacteria" id="AGZ39254">
        <property type="protein sequence ID" value="AGZ39254"/>
        <property type="gene ID" value="AFR_04825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.974"/>
    <dbReference type="eggNOG" id="COG0736">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089696_0_0_11"/>
    <dbReference type="OrthoDB" id="1893660at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00516">
        <property type="entry name" value="acpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACPS" evidence="9">
        <location>
            <begin position="4"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="12949" checksum="9056FFFE578EE210" modified="2014-01-22" version="1">MIVSVGIDVVLVERFGRALERTPLLADRLFTDSERLTSSGNPRSPESLAARFAAKEAVAKALGAPVGMRWHDCEVVTDPDGRPWLTVSGTVAAVAAERGVNRWHLSLSHDGGIASAMVVAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS43</accession>
    <name>U5VS43_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_06640</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359148.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06640"/>
    <dbReference type="EnsemblBacteria" id="AGZ39617">
        <property type="protein sequence ID" value="AGZ39617"/>
        <property type="gene ID" value="AFR_06640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1355"/>
    <dbReference type="eggNOG" id="COG1177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_3_0_11"/>
    <dbReference type="OrthoDB" id="1264541at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="80"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="30059" checksum="502F5F4EC018F558" modified="2014-01-22" version="1">MTQAVLEDAPVVKPRRSRLTGGRALYIYTWVIMAWLILPVLFMIAFGFNDTPGRYNQTWEGFTFKWYGRVFDLDELTKALVVSLGVAVVSSFVAGALGTGIGYALGRFRFRGSGTLNLIMFATISSPELVMGTSLLTLFVSVGAGLGVVTITIAHIMFSISFVAIVVRARVLTLDRSIEEAAADLGANAWTTFWRITFPIILPAVGAGMMLAFALSLDDFVVTNFTAGTTTTFPLWIWGATRVGLPPQVNVMGTLIFMAGVLLAVASSIRSRPRKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSF5</accession>
    <name>U5VSF5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c-type biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357499.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01335"/>
    <dbReference type="EnsemblBacteria" id="AGZ38556">
        <property type="protein sequence ID" value="AGZ38556"/>
        <property type="gene ID" value="AFR_01335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.267"/>
    <dbReference type="eggNOG" id="COG0785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053225_2_1_11"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="DsbD" evidence="2">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="DsbD" evidence="2">
        <location>
            <begin position="81"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="28338" checksum="FB0BBDB1986BB54C" modified="2014-01-22" version="1">MGETFADVASNGPLLLAIGAAAIAGLVSFLSPCVLPLMPGYLSYVTGLAGADLDSALGVAPKEEVAAAGSVAVKTKPPETQTAGRGRVLAGTVLFVVGFAVVFMLLATLVANIGLALKTHERTLNIVLGSLIIVLGLVFLGVVPGLQGQARVKWIPNAGLAGAPVFGALFGLSWLPCTGPTLGAVLGLATTSGQTDRAVVLALAYSLGLGIPFVLFGLFFRRLLGVFKAVRRNSRWVTRVGGVLLIAVGIALVTGGWNNFLIWLVTTFNFDAGTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSH9</accession>
    <name>U5VSH9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative globin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359303.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07415"/>
    <dbReference type="EnsemblBacteria" id="AGZ39772">
        <property type="protein sequence ID" value="AGZ39772"/>
        <property type="gene ID" value="AFR_07415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1513"/>
    <dbReference type="eggNOG" id="COG2346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103526_3_1_11"/>
    <dbReference type="OrthoDB" id="1697277at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="SSF46458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="141" mass="15929" checksum="53AF89FBAD9987CC" modified="2014-01-22" version="1">MSAANEQQEASFFEAVGGEPTFRRLVNAFYEGVAKDPILRPMYPEGDLGPATERMTLFLMQYWGGPSTYSETRGHPRLRMRHAPFQVGPAARDAWLHNMRAAVDSLHLPEPHHSALWDYLERAAYFMVNAEDPQPGGQLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSI1</accession>
    <name>U5VSI1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359355.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07675"/>
    <dbReference type="EnsemblBacteria" id="AGZ39824">
        <property type="protein sequence ID" value="AGZ39824"/>
        <property type="gene ID" value="AFR_07675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1565"/>
    <dbReference type="eggNOG" id="ENOG5031Y41">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1615498_0_0_11"/>
    <dbReference type="OrthoDB" id="2081135at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665272" evidence="1">
        <location>
            <begin position="27"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="175" mass="17571" checksum="DB10A10ABDA60416" modified="2014-01-22" version="1">MRRLLCATAVSGALLLGAGCSTDKSAETTTVAPTSAASAAAGPAEGAVTASAGAGQAGAGDAALSGNTDAICQQAAKTGGDSAKNFAEDRKLLKAAKSSKDKDQVAKAKEKATRDVENYSFALKDMSELTTDKKLKATLADMSKQVAALKGDVTKLDSAQLTKLNDTLGKACGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSM8</accession>
    <name>U5VSM8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07900"/>
    <dbReference type="EnsemblBacteria" id="AGZ39869">
        <property type="protein sequence ID" value="AGZ39869"/>
        <property type="gene ID" value="AFR_07900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1613"/>
    <dbReference type="eggNOG" id="ENOG50320QV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107443_0_0_11"/>
    <dbReference type="OrthoDB" id="2094022at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="25119" checksum="48E549C55DFABF08" modified="2014-01-22" version="1">MTGMGMRYERYDDAEVTEFRVVAAPDELVPALQRSAFTERDGCWVRSCPAGTPHVDDAWVNFQRLAEPWLRQLAGLEPVLWEETLDLVCRRLEGIDWWLTGSAALAARGMAITPGDLDLVVSGADSHRAGELLLDGLVEPVAAVDWFCDWFGRAMFGARVEWAGGVGPAADQPEITDFGPHAAARLDKVRWRDHDIRVPPLDLQRAVNVRRGLTERVRLIDQFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSP0</accession>
    <name>U5VSP0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38646">
        <property type="protein sequence ID" value="AGZ38646"/>
        <property type="gene ID" value="AFR_01785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.361"/>
    <dbReference type="eggNOG" id="ENOG5033BVD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2025642_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="13620" checksum="ACB007E2DC3BE2F2" modified="2014-01-22" version="1">MRDGYVVTWHGREYDAAPDGENVRIYAPEPGDGFEEARPGRYVRVLEPGEFGDLVYVRTTCSWRGEPFIVLAEADSWLRLEYTGGRAPVAHGLGLEEFDFGVYQGWAPAHEVTDLYEHRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSU6</accession>
    <name>U5VSU6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08745</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359569.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08745"/>
    <dbReference type="EnsemblBacteria" id="AGZ40038">
        <property type="protein sequence ID" value="AGZ40038"/>
        <property type="gene ID" value="AFR_08745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1779"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040011_1_1_11"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="176"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="417" mass="43565" checksum="4941E7A25BD45CD2" modified="2014-01-22" version="1">MSVTQGAPAALAEPVVPVRRSWIGLLFAANLGLWMAFFTPVQVLLPEQISEIGIVGKEAALGWVTALGALVAIVVNPAAGAFSDRTRLRIGGRAYGRRHVWTLGGAAISCASLLLLSGQQTIAGVALGWAGTQLGLNIMLATLTAAVPDRVPVGQRGAVSGWIGMPQALGLVLGAVLVTAVVTGVASGYVVMAVVLVVLAVPFALLTPDDPLPVEAERPRSRTKIWRVFRENPDFAWAWGTRFLVQLGNALGTLYLLYFLTDRVKLADPDTGLLIMILLYTLGMVATAVIGGRLSDRSGKRKIFVIWSGVVIAVAALLLAIWPTWPASMVAAVLLGAGYGVYLAVDTALITQVLPAAVDRAKDLGVINIATAAPQVLGPAIAAPLVTHLGGYPTLYALTAVVTLLGAVMVVRIRAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSY4</accession>
    <name>U5VSY4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359510.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08450"/>
    <dbReference type="EnsemblBacteria" id="AGZ39979">
        <property type="protein sequence ID" value="AGZ39979"/>
        <property type="gene ID" value="AFR_08450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1720"/>
    <dbReference type="eggNOG" id="ENOG50335GU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160021_0_0_11"/>
    <dbReference type="OrthoDB" id="1785527at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003489">
        <property type="entry name" value="RHF/RaiA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02482">
        <property type="entry name" value="Ribosomal_S30AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="SSF69754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="131" mass="14675" checksum="00A7CCD1C7761486" modified="2014-01-22" version="1">MSAVANPATVEDCLRVGAGFTQGDRNWIAEQFATLDARLAAFHADTTELEVSVKDRAARGQKVTLECWIAGRQKVVTTSLEDDLHAALNDVRDDLRRKLNDAKTKQEPRHNRHLRDNSLPEETELPEALQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSZ7</accession>
    <name>U5VSZ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative N-acetylneuraminic acid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02805"/>
    <dbReference type="EnsemblBacteria" id="AGZ38850">
        <property type="protein sequence ID" value="AGZ38850"/>
        <property type="gene ID" value="AFR_02805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.563"/>
    <dbReference type="eggNOG" id="COG2089">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040465_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006190">
        <property type="entry name" value="AFP_Neu5c_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036732">
        <property type="entry name" value="AFP_Neu5c_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013132">
        <property type="entry name" value="Neu5Ac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020030">
        <property type="entry name" value="Pseudaminic_synth_PseI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03102">
        <property type="entry name" value="NeuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51269">
        <property type="entry name" value="SSF51269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03586">
        <property type="entry name" value="PseI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50844">
        <property type="entry name" value="AFP_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AFP-like" evidence="1">
        <location>
            <begin position="291"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="346" mass="37200" checksum="9609ED62264A1B87" modified="2014-01-22" version="1">MIVNDVEIGAGRRPYIVAEMSGNHNGSLDRALALVDAAADAGAHAIKIQTYTADTMTVDVKHPRFQISQGHELWGNEYLYQLYERAHTPWEWHEAIFERARGRGLTPFSSPFDRTAVDLLEKLDAPMYKIASSEITDLPLIRLCASTGKPLIISTGMASVQEIAAAVDAARGAGCQDLVVLSCTASYPAPPEHTNLRRIPVLAEAFRVPIGLSDHTLGIGVPIASVAFGACLIEKHVTLDRGDGGVDSAFSLEPAELSSLVVESERAWQALGTTSIGPTEAEKEGLRFRRSLYVVADVRAGDTVTEDNVRSIRPTGGLAPDAIGMVLGRTFTQDAAKGTPLTWDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT76</accession>
    <name>U5VT76_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359548.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08640"/>
    <dbReference type="EnsemblBacteria" id="AGZ40017">
        <property type="protein sequence ID" value="AGZ40017"/>
        <property type="gene ID" value="AFR_08640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1758"/>
    <dbReference type="eggNOG" id="COG3386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056731_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666051" evidence="1">
        <location>
            <begin position="26"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="32069" checksum="3CE5CA7D8C50C6E8" modified="2014-01-22" version="1">MSRRTLAIVTATALAVVVGAAPAEASHRAPAPTTIQLPDGFQPEGIAIGKAPYAYFGSRVDGDIYRVSLGSGRGKVISQGPGTPSLGLKLDNRGRLFVAGGNGGDARVLDAGSGKVLKSYTLQTGTAFINDVILTGGAAWYTDSANPVLFKLPLGRHGKLPAEATRVPLTGAITYQTGNNANGITATPDGRGLIIVQSNTGKLFKSSYSGVTTEIDLGGENVLNGDGLWLRGSTLYVVQNRLNVIARIQLDKHATKGTVVSRTTDPRFDVPTTIAEYGKRFYLPNARFTTTPTPTTPYTAVAVARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT96</accession>
    <name>U5VT96_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type 11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08825"/>
    <dbReference type="EnsemblBacteria" id="AGZ40054">
        <property type="protein sequence ID" value="AGZ40054"/>
        <property type="gene ID" value="AFR_08825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1796"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_2_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="51"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="283" mass="29780" checksum="A05E61AE6ED9842E" modified="2014-01-22" version="1">MTEAYLDFDRHEREMWAGRASTYERSFAPLCAGVRDQVLDLAGVSGGKRLLDVGTGRGTLAAAAAGLGALVTAVDAEPSMVEAAGRNVPGAEVRVARLPDLPFEARSFEAVTANFVLNHVGDPAAAARELVRVTATGGRVAASIWPFPAPPLQAFWGEVVAGSGVVPPGGSPRVEEALDFPRTAAGLTTLLHDAGLDGVRVETVTWTHHFDPEDWWSGPAGGLSTLGTLLRSLPAESVTRIRASYDRAIDSRRDSNGRVGLSTTALLGSGTRLEHHTRASQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTG5</accession>
    <name>U5VTG5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">Transcriptional regulator MraZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mraZ</name>
        <name evidence="10" type="ORF">AFR_09970</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Forms oligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MraZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360009.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09970"/>
    <dbReference type="EnsemblBacteria" id="AGZ40283">
        <property type="protein sequence ID" value="AGZ40283"/>
        <property type="gene ID" value="AFR_09970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2032"/>
    <dbReference type="eggNOG" id="COG2001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107907_0_5_11"/>
    <dbReference type="OrthoDB" id="1684767at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009295">
        <property type="term" value="C:nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16321">
        <property type="entry name" value="MraZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16320">
        <property type="entry name" value="MraZ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01008">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003444">
        <property type="entry name" value="MraZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035644">
        <property type="entry name" value="MraZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020603">
        <property type="entry name" value="MraZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035642">
        <property type="entry name" value="MraZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038619">
        <property type="entry name" value="MraZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34701">
        <property type="entry name" value="PTHR34701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02381">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00242">
        <property type="entry name" value="TIGR00242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="10" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="9">
        <location>
            <begin position="5"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="SpoVT-AbrB" evidence="9">
        <location>
            <begin position="76"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="16044" checksum="93E20ED7E6E194B7" modified="2014-01-22" version="1">MFLGTHTPRLDDKGRLILPAKFRDELAGGVVITKGQERCLYVFPMPEFQRIAGQLREAPVTNKAARAYSRVFFASAHDEIPDKQGRVTIPGHLREYAALDRELVVIGASTRVEIWDKQSWEQYLSASEDEFADIEEGVLPGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUA9</accession>
    <name>U5VUA9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">50S ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 7" type="primary">rplQ</name>
        <name evidence="7" type="ORF">AFR_04660</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Contacts protein L32.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358224.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04660"/>
    <dbReference type="EnsemblBacteria" id="AGZ39221">
        <property type="protein sequence ID" value="AGZ39221"/>
        <property type="gene ID" value="AFR_04660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.941"/>
    <dbReference type="eggNOG" id="COG0203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074407_0_0_11"/>
    <dbReference type="OrthoDB" id="1838961at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_L17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="PTHR14413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="SSF64263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="20667" checksum="F996A7694BC5F3D5" modified="2014-01-22" version="1">MPTPTKGPRLGGSPAHEKLLLANLATELFRHGKIKTTETKAKRLRPLAEQLITKAKRGDLHSRRRVLGVVKDKDVVYSLFEQIAPRYTNRPGGYTRITKTGPRKGDAAPMAVIELVEELQVAATTAPAKKAARKAAAQQDKVEALAREDEAPATTTTSETAGDQDAEPPVNASGDKGAEGPGDKAEGEDPDAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUW2</accession>
    <name>U5VUW2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360562.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11415"/>
    <dbReference type="EnsemblBacteria" id="AGZ40572">
        <property type="protein sequence ID" value="AGZ40572"/>
        <property type="gene ID" value="AFR_11415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2327"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124277_3_1_11"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="20"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="14751" checksum="0471B88B3126B190" modified="2014-01-22" version="1">MSLSHHQIFERYVYAGAILRDAGAVAALFTEDGVFEAPLVPAGHRLPRRLVGRAAIEEGLAVYHRDMTYPGVVDTERSTYVLHATTDPDVFVAEIDTVFETPAGQHTTMSLAQIFRLRAGHIAVLRDYFAPPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV39</accession>
    <name>U5VV39_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase-like FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360793.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12240"/>
    <dbReference type="EnsemblBacteria" id="AGZ40734">
        <property type="protein sequence ID" value="AGZ40734"/>
        <property type="gene ID" value="AFR_12240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2488"/>
    <dbReference type="eggNOG" id="COG3467">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123922_3_1_11"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019920">
        <property type="entry name" value="F420-binding_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03618">
        <property type="entry name" value="Rv1155_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="10"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="14911" checksum="E068F5EA52B6069B" modified="2014-01-22" version="1">MTSQPEIIPGKFLDLLERPLIAHLATVREDGAPQVNPMWFSWDGAFIRFTHTNFRRKYRNVTANPAVSLSIVDPANPYRYLEVRGVVERIDADPAGAFFMELAQRYGAPFGAEPPADAAARVIFVVRPTATSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVL8</accession>
    <name>U5VVL8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD_binding_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360911.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12830"/>
    <dbReference type="EnsemblBacteria" id="AGZ40852">
        <property type="protein sequence ID" value="AGZ40852"/>
        <property type="gene ID" value="AFR_12830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2606"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_20_3_11"/>
    <dbReference type="OrthoDB" id="867226at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="5"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="497" mass="52628" checksum="611B849A543FBF7E" modified="2014-01-22" version="1">MTTDTVLIVGAGPTGLTLACELARRGIPFRLIEAAKDPQPGSRGKGVQPRTLEVFDDLGIVDRVLAHGRMAMPIRSTGPDGRVTLSGAEPAGKNPGIPYPASLITPEWRVKEALRLRLAELGGAVEFGTALAGFEQSDEGVSAKVVSGGVTATITAHWLVGCDGGHSVVRKLAGVAFVGETREDVRMIVADVEVDGLDCDAWYVWRHEEGLVNLCPMPSTSVFQYQAGVAPGERPELDVATMQAILERRTGRSDIRLHEPRWSSLWRANIRLADRYREGRVFLAGDAAHIHSPAGGQGMNTGIQDAYNLGWKLAAVAGGTASPALLDSYEAERRPVAVGVLALSNARLEQAIREKTVPTRRDADTTQLGISYRGSSLARDDRAATAPLRAGDRAPDAVLTTVQGERRLFDLTRGTHFTLLSFGSTPAIGATPNDVRVLGVGPRPAGPDDIADTDGGLAAAYGATGNTLVLIRPDGYIAMISDSGNGAALRRHLSAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVV8</accession>
    <name>U5VVV8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uma2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359312.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07460"/>
    <dbReference type="EnsemblBacteria" id="AGZ39781">
        <property type="protein sequence ID" value="AGZ39781"/>
        <property type="gene ID" value="AFR_07460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1523"/>
    <dbReference type="eggNOG" id="COG4636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076312_4_0_11"/>
    <dbReference type="OrthoDB" id="2040599at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06260">
        <property type="entry name" value="DUF820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012296">
        <property type="entry name" value="Nuclease_put_TT1808"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008538">
        <property type="entry name" value="Uma2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05685">
        <property type="entry name" value="Uma2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uma2" evidence="1">
        <location>
            <begin position="23"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="20372" checksum="49D7D3F6FC9E4CFC" modified="2014-01-22" version="1">MAIGTNSLVRWTAPDGPWTEPDLHLFPQDGHRYEIVDGSLHVTPPLDGPHETVVRALVTVLRGAAPDGWWVCARLGIESGTSNLIPDVTVLRPHSSGAIWADPADVALVVEVETATSRRFDRLLKPTLYADAGIETYWRIEPDPEPVLHTYALGPDGYVHEKTMQGADLVKVDAPFPVRVSPSAWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW68</accession>
    <name>U5VW68_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08455"/>
    <dbReference type="EnsemblBacteria" id="AGZ39980">
        <property type="protein sequence ID" value="AGZ39980"/>
        <property type="gene ID" value="AFR_08455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1721"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="2">
        <location>
            <begin position="55"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="523" mass="55442" checksum="06A0F087657AEFAA" modified="2014-01-22" version="1">MSRGFAQWGMSLTLAPGTAWNDVLARVAAAAPEAVAADRLRNLYAGRWHDGGTPAPLRTPVDGTLLTSLAKVDASTAAEAVRAATAAHLLWAGTPLADRKARVAAAVEALTLHRDLLALTLVWEIGKPWRLACADVDRALDGVRWYAEQIDRQTAGREPLPGPVSNIASWNYPMSVLVHAELVQLLAGNAVIAKTPSQGGAVCLTLAHALMARAGLPVTLLSGSGEELSEVLVRAPEIGAVAFVGGRSNGGKVAAALLDSDKRHFIEQEGLNAWGIWDFSQWDLLAGHLRKGFEYGKQRCTAYPRFVVQRDLVDAFLDVYLPVVKAVRFGHPLAVETAGDPLPELDLGPLISAAKAGELRRKVDEAVRGGAIPLHRGSGSAGRFLEGQDTSAYVAPTALLAPPGRSRLMHAEPFGPVDTIIVVDTQDELLSAMNASNGALVASLAVDDEELGAKLAQDVHAFKVGINRPRSRGDRDECFGGRGASWKGAFVGGDLLVHAVTIGEPGERLYGNFPDYSSYPPGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWD8</accession>
    <name>U5VWD8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_15180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15180"/>
    <dbReference type="EnsemblBacteria" id="AGZ41318">
        <property type="protein sequence ID" value="AGZ41318"/>
        <property type="gene ID" value="AFR_15180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3082"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="221" mass="24405" checksum="F36FA9DB67ABF202" modified="2014-01-22" version="1">MTMLSTQVYAELRDAILRGDFAPGDALKPQELAHRHGVSLAVVREALTRLVGDGLADRLVNRGFAVPLQSDQRWQDIAEARRTVEPVMLRMAIDRGDLDWEARVRAAHHRLARTPPLQPGEGPHVSAEWSRAHHDFHRALLEGCGNPVLLETFDRLWLASELSRRWSGAGNPHRDYLEEHRLLEEAALARDAGAAAGLLERHVTRTAAALVAGEASGRPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWU9</accession>
    <name>U5VWU9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361408.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15335"/>
    <dbReference type="EnsemblBacteria" id="AGZ41349">
        <property type="protein sequence ID" value="AGZ41349"/>
        <property type="gene ID" value="AFR_15335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3114"/>
    <dbReference type="eggNOG" id="COG5373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840808_0_0_11"/>
    <dbReference type="OrthoDB" id="2042592at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="14369" checksum="393B1D4B257B5871" modified="2014-01-22" version="1">MKVVKLAAGMAIGYVLGSRAGREKYEQIVETVRKVNNQPGVVRAKEKATEEVGAKPAGSTPEPKAATPSEPKPAPTSEPKAATTSEPKATSEPKATSEPKSTTTSEPKADAVPRPPRRKPAAATTTTTVVTPADASPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWZ5</accession>
    <name>U5VWZ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361371.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41312">
        <property type="protein sequence ID" value="AGZ41312"/>
        <property type="gene ID" value="AFR_15150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3076"/>
    <dbReference type="eggNOG" id="COG4334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133265_1_0_11"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016888">
        <property type="entry name" value="UCP028498"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10012">
        <property type="entry name" value="DUF2255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="115" mass="12471" checksum="6387872A5945CC61" modified="2014-01-22" version="1">MTPEELTLVCAAEELEIAARRWTPIWVVCVGAHPYVRSWYRRDTGWFAHVLKAGRAAVRVPGLQIDVTVEDIGALPAGLRSDIDAAYRTKYGRYGAGGVGPMVTDAAAATTLRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX33</accession>
    <name>U5VX33_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA_12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361411.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15350"/>
    <dbReference type="EnsemblBacteria" id="AGZ41352">
        <property type="protein sequence ID" value="AGZ41352"/>
        <property type="gene ID" value="AFR_15350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3117"/>
    <dbReference type="eggNOG" id="COG1112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_307098_0_0_11"/>
    <dbReference type="OrthoDB" id="616258at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_12" evidence="1">
        <location>
            <begin position="602"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="913" mass="97626" checksum="29F6C930BD761256" modified="2014-01-22" version="1">MLPSTVLAVLAELAPAGADRVLDVTRGGQPLAWLDDAERAPRRAETDRLAALAALHRDERILRRGWGFLAGRTEVDGKVRKVRVPLMSQPVQLERGLTGYRVVPIGDVEITPLVADRQLAAAFEAAPGLAAPGWLDAIGSRAWLWSAAQATGLPVDAVVADRRKLPNDRIVLVATAGLFVVRDVYGPGLADSLRTWSRRDLSGTALAAVYGDERTDRSDDTPVVSPLPLTEAQARVVARVRTAPVTVVSGPPGNGKSHTVVAAALEVVHRGGSVLVATQSPHAAEVLASLLGRYPGPVPVLFGDAQRRDDLAAQLAAGADRGYSDRELRADADSVQLALRGVGQERARITEALDEEVRAAALPRWEPLLPELAADVPGAFTGVRTDDFDRPGLRGWWARRRLGVRGGVPAERVRAALTAAAAQLSAARLAATGGTDLDRRWSTLRYLEDGLARTAGEAMRRAARSEKRWDREARRGVAALATALRAGRNQRRELLARMDAAPLVRALPLWIGTVADVEDLLPAAPGLFDLVVIDEASHVDQVRAAPVLARARRALIVGDPRQLRFVSFVSTVDINEVLARHDADDRLDVRRVSTYDLATAAAPATWLAEHHRSTPHLIGFSARRFYDDRVAVMTRTPAAELADGIDVVRVPAPGSLAAEVADGIDVVRVPAPGSLAAEVAAVLEVVAAQAGAGTRSIAVISPFRAQAEALEAALVAAYPVDRIEELGLRVGTVHAFQGSEADVVVVSLGLLPGDPAGRRRFVADPHLFNVMVTRARHRMVVVTSLGTPDGLLADYLTYADTPPGPAGQEVVEANPWPAALHEELRRLEVEVRPRYAVGPWTIDLCIGPAEAPIGVFCEVHPDGTGAHLARRQALHRAGWRLADAFASRWAGDPRRAALELAQLKATTELTPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXD9</accession>
    <name>U5VXD9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360229.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10530"/>
    <dbReference type="EnsemblBacteria" id="AGZ40395">
        <property type="protein sequence ID" value="AGZ40395"/>
        <property type="gene ID" value="AFR_10530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2146"/>
    <dbReference type="eggNOG" id="COG1287">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016537_0_0_11"/>
    <dbReference type="OrthoDB" id="796556at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="598" mass="64263" checksum="2B1A67866EECFBF5" modified="2014-01-22" version="1">MPAVDEVLDEPGLSVEAEPKKRAYRYADVWAAISYLALGVLVLIQLWISPGGRVLSSNDDDHGFFSFVMAHGERVVFHGANPLFSDRMNVPDGVNMMANTTVLGLSVPMAPVTHFFGVGVSVVLLLTLGLAGTAFAWYWVLSRHLVTSRAAAWIGGLWCGFAPAMVSHANGHINFVSLFVVPFIVLQVLRLREPGRVVRGGIILGLLVVLQVFINEETLLFTALTLGVFVIAYAALAWRTAVGYVRRMAAGLGVAIVTALVPLAYPLYFQFTGPQSYSGQPFEPGKFVTDLLALGAYARQSLAGNGAIARALSVSPTEDNTFFGVPMLVMIVVSVVLLWRNIAARSAAIAGLVLLIISMGPELRVGGYNTGIPLPFALIAHVPIIDLVSVTRFGMVPATIIGLLLALAADRAPLLPHRKRVAFGIGLALAIVPVLPKPLPVVTADPLPPFIAQQMWRDYVPGNRTLVTVPLPEVTTGRAGMRWFALSGLDYSVPRGYFMGPANPPADKTGSWNAAPRPTSDLLRIAGAYGRMPVVTGADRRAAITDLTYWRAAVVVLVPGTPNHDLLQTLVSDLLEKEPQQVGGVQVWDVRDQVRPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXG9</accession>
    <name>U5VXG9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_16050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361564.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16050"/>
    <dbReference type="EnsemblBacteria" id="AGZ41492">
        <property type="protein sequence ID" value="AGZ41492"/>
        <property type="gene ID" value="AFR_16050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3257"/>
    <dbReference type="eggNOG" id="ENOG5031K3Q">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1227772_0_0_11"/>
    <dbReference type="OrthoDB" id="1671042at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="25059" checksum="99A33BDB23D4DBEA" modified="2014-01-22" version="1">MAQRKLDVVVVRWYERLDRSPDVVPRWLAAARDHLPEATPRRYGDSEPLRDRFDRDGEAGLARAYAEADSLLFLAGTPPVFHASLSTRGTGRLRGGPVGAHTLHAELDPADERLHRFALALTHPGTIYVSASVSGGETLDRGTLYGPGDRPAEPYLAPLGFWLGLPPTRPAWHFFGTAYTRALPADLGTGWVPERFQARLQEDDIAQRHAPRLPRGLGRSRLRFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXV0</accession>
    <name>U5VXV0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S58 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41818">
        <property type="protein sequence ID" value="AGZ41818"/>
        <property type="gene ID" value="AFR_17700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17700"/>
    <dbReference type="eggNOG" id="COG3191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044458_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005321">
        <property type="entry name" value="Peptidase_S58_DmpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36512">
        <property type="entry name" value="PTHR36512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03576">
        <property type="entry name" value="Peptidase_S58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="27617" checksum="440D0EE938B9BD89" modified="2014-01-22" version="1">MTVILPPAGTVGSGEVRGGAPATREFALLDPARLVDRVDAVVLSGGSAFGLSAADGVMGLLRDRGAGFPTPAGPVPIVVGMSIFDGSVRTGPPGAEAGRAAAEAALNSADLGGPAVGGPAVGGAGLRSGRIGAGMGASTGKWRGLLDPGGLGRAQRDVDGVRVEAVVVVNAWGDVLGRDGEPLFGAGEDAAHPGTPFGLDNTTIAVLLTTARLSKADCFLLAQSGHTGFARTIHPAHSRYDGDAVVALATGESEAEADLDLLRAVAVDVMADAIRSAVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY37</accession>
    <name>U5VY37_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">von Willebrand factor type D protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18175</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362286.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18175"/>
    <dbReference type="EnsemblBacteria" id="AGZ41913">
        <property type="protein sequence ID" value="AGZ41913"/>
        <property type="gene ID" value="AFR_18175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3689"/>
    <dbReference type="eggNOG" id="COG0810">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_326697_0_0_11"/>
    <dbReference type="OrthoDB" id="1048626at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665334" evidence="3">
        <location>
            <begin position="30"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="4">
        <location>
            <begin position="430"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="881" mass="90707" checksum="5F6DD693B1F6EFE5" modified="2014-01-22" version="1">MLSSVVRSMITGAALAAAVIAGWGSPAAAADSGVHLDVTTPEAVHEPGAGVPLRLVVTNRHDEPCVLASVADASVSVLAATRDGRPLTPSFARALIGSGSGTILTRYARSIAPGDTASFTLDAVQPQGLTTVTPLRDQSALETTWPTADAGTYRVDLAYQVPAIVGADACTGRSNVVTVAFEVGEPGPSRLLLALLTAGALLVILLLVVVILLVVRRRSTAAPVLVLLLVVAGTLTLPGRARADIVYSGGSDTASNVVYGGCAGLIGDFNPDLWEQLDGRGKGAPTVRIYQWIYSERVQLGGDSRSSLIKWDYRDKSHIEGEAAGVNYDPCAELYHELVHAQDAANNKLSDAKCGDSNVFVDEIRATYAENAYRTSHGLKPRTGYGGIALPATLDDCAPGKKTKKDKTAKPAPGTSAPAKKARRCASGSCAVSNGDPHLTTFDGQQYSFQAVGEFVAAGDPGIQVRQAPLPGYRTVSVNSAVALRAGDTRLGFYLVDGHIVVHRDGDPATVETGDTPLPGGAVLGRSVDQYTGDIYDVTWADGAVASVWRTGAYGLVLTVAPAANRRDDRSGLLGDFPGTAQLYTAFADKWRVTDDTSLFDYADGQNTATFTDRAFPSESEPTQSPARAEAASVACSAAGVTGATDLANCVLDVSLTGSADFATIASDIAALVSAPAVGDPASSVASVEQAGAVGHQTFAGKEGERAYIEVESSSFDDQCGSLTVRAPDGRTIGLGCVKTGGVIDGAALPVDGEYRIDVDPDGDAVGEVRLGIVVSKDQREKISIGGGPVTATIGDPGGLARFTFTGTAGQTVAVDASGASIPDQCGVLQIQASDGAGLAEGCLARGAGRLEDVTLPRDGTYRLVVDPARAGTGEVTLQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYI5</accession>
    <name>U5VYI5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CspA family cold shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12125</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360770.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12125"/>
    <dbReference type="EnsemblBacteria" id="AGZ40711">
        <property type="protein sequence ID" value="AGZ40711"/>
        <property type="gene ID" value="AFR_12125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2466"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_0_3_11"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="4">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="74" mass="8047" checksum="0569DE8D8158A778" modified="2014-01-22" version="1">MAQGTVKWFNSDKGFGFITPDGGGADVFVHFSSIQTNGYRSLDENQRVEFEITQGQKGPQAAQVRPLGASREIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYZ2</accession>
    <name>U5VYZ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_13320</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13320"/>
    <dbReference type="EnsemblBacteria" id="AGZ40950">
        <property type="protein sequence ID" value="AGZ40950"/>
        <property type="gene ID" value="AFR_13320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2704"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="108"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="108"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="23428" checksum="26EFBAE5D7A879BC" modified="2014-01-22" version="1">MLRRYLVAEGHETAVVHDGRTALERVRQRAPHLLVLDVMMPGLDGLSLCRILRRESDVPILMLTARSSENDLLTGLDLGADDYLTKPYSPRELLARTRALLRRSRGSTPVLTIGRIVVDTTRHEVSADGRPVECTPGEFALLAAMAAQPDRVFTRAQLLQHTNGVERDSIERTIDVHVLNLRRKIEPNPRRPSRLLTVYGLGYKLTAGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ50</accession>
    <name>U5VZ50_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavin-dependent oxidoreductase, F420-dependent methylene-tetrahydromethanopterin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19930"/>
    <dbReference type="EnsemblBacteria" id="AGZ42258">
        <property type="protein sequence ID" value="AGZ42258"/>
        <property type="gene ID" value="AFR_19930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4044"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_6_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022480">
        <property type="entry name" value="F420_MSMEG2906"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03856">
        <property type="entry name" value="F420_MSMEG_2906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="252" mass="27585" checksum="6F2F129CB3916CF0" modified="2014-01-22" version="1">MQIQPQHASYASIRRAAAQAEELGVDILFNWDHFYPLYGDPDGLHYECWTMLGAWAEATSRVEIGALVTCNSYRNPELLADMARTVDHISDGRLILGIGAGWFEKDYDEYGYEFGTPGSRLADLAQALPRIESRWAKLNPPPTRKIPVLIGGGGEKKTLRLVAQHADVWHSFGDAETVERKLGILRQHCADVGRDAGEIEVSAGVDGASDPAVAGAAMVAKGVSLLTLNAGGPDYDLEPLKRWLAWRDEQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZH6</accession>
    <name>U5VZH6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361169.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14140"/>
    <dbReference type="EnsemblBacteria" id="AGZ41110">
        <property type="protein sequence ID" value="AGZ41110"/>
        <property type="gene ID" value="AFR_14140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2869"/>
    <dbReference type="HOGENOM" id="CLU_2630115_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="77" mass="8523" checksum="810588CD039C96A0" modified="2014-01-22" version="1">MSDEIIRDDRPDRDTSDNLPTDAADPHQPAARRRQQIGSAGGGPYAAGRPEDQHPDRPDEDEPARSSPADREQTFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZJ1</accession>
    <name>U5VZJ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362681.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42309">
        <property type="protein sequence ID" value="AGZ42309"/>
        <property type="gene ID" value="AFR_20185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4094"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_11"/>
    <dbReference type="OrthoDB" id="1209997at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="27585" checksum="4EEDD46024A697CC" modified="2014-01-22" version="1">MRLDLSGRTALVTGSTQGIGLAIATGLAEAGARVAINGRDPEKVRAVLPERGDFVAAPADLTTDEGVAALLEQLPSVDILVNNLGIFGAQPALEITDAEWRRYFEVNVLTGVRLTRAYLPGMKERGWGRIQYIASDSAVVVPAEMIHYGMTKTALLAVSRGFAKEAAGSGVTVNSVIAGPTHTGGVEDFVYELVDKDLPWEEAQREFMRLHRPQSLLQRLIEPEEIANMVVYLSSPQASATTGGAVRVDGGYVDAILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0G5</accession>
    <name>U5W0G5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362956.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21570"/>
    <dbReference type="EnsemblBacteria" id="AGZ42584">
        <property type="protein sequence ID" value="AGZ42584"/>
        <property type="gene ID" value="AFR_21570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4374"/>
    <dbReference type="eggNOG" id="ENOG5033T88">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089977_0_0_11"/>
    <dbReference type="OrthoDB" id="1571466at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="238" mass="24603" checksum="58ABBA6A3214294F" modified="2014-01-22" version="1">MSAETTGPPFTFPGPWLGGVSLIAGPLLLLAGTLLRLGVPFFFPHQLAAYQQNPTLTATAYALFLAGTIALWPGIVAVAMRVGATRPAWALWGGSLVMAGLFARTFHYGVNTFAFSLVDSAGLGAASPAVSAYYRYPEWVVSSLTLAVMTGWVVLAIGCFLSGTLRLLPAIALALMSGLMIGVLKGSTWASAVEVAGLTVALVPLGVAFLRNAGRPSRSAVPLVLLFVVGSIVLGQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0U3</accession>
    <name>U5W0U3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23040"/>
    <dbReference type="EnsemblBacteria" id="AGZ42878">
        <property type="protein sequence ID" value="AGZ42878"/>
        <property type="gene ID" value="AFR_23040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4669"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="25800" checksum="D9B4D467D23F57C7" modified="2014-01-22" version="1">MTSRRPEPLEGAVALVTGAGDGVGAATARRLAREGAAVALVGRRRDRLDTVVGEIKLAGGHAVAMDTDITEPDRAYEAVQDTMDRFGRLDVLVNTAAIMLLNSALHAGVEEWERMIALNVRALLHVTHAAVPFLIDAASTSPRQVADLVNVSSTAGRVARPGSSVYNLTKFGLNGFTESLRQELIPERVRVSVVEPGTEPVIAGAEALWADDIADAIGYIVTRDRRVAVNEMLVRAGDRTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W159</accession>
    <name>U5W159_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361725.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16740"/>
    <dbReference type="EnsemblBacteria" id="AGZ41626">
        <property type="protein sequence ID" value="AGZ41626"/>
        <property type="gene ID" value="AFR_16740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3394"/>
    <dbReference type="eggNOG" id="ENOG50332CB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057517_1_0_11"/>
    <dbReference type="OrthoDB" id="1331840at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666270" evidence="2">
        <location>
            <begin position="32"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="33366" checksum="EBD6390B64ACC06B" modified="2014-01-22" version="1">MNRYTTPVRLAAALALCLVAAGCSDDKQPGAASDALTVNSPPVVGAAPQLDLLDGKVFPVEAYKLTPEKTRQLNNAQTVLANKCLARFGFPPSLREVPASQPMDASNSRRYGITDGAVAAKYGYHQPGGSDGQTSRPPAEPVTKELSLVLYGAPDPTGTGKQTGTAANGAEIPAGGCMGESKSKLSADAYERVDPGQFASDIELAAGKQADADRRVTGKFAEWSACMKTDGFDYSDPFQPLDGEILKSSLAQPVPSALEIRTAVADVECKQKVNLVGVWFTVEAAYEEKLIEKNQERLADIKKAIDAMLQSAAGVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W185</accession>
    <name>U5W185_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_23170</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363416.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23170"/>
    <dbReference type="EnsemblBacteria" id="AGZ42904">
        <property type="protein sequence ID" value="AGZ42904"/>
        <property type="gene ID" value="AFR_23170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4697"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_4_0_11"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="9">
        <location>
            <begin position="23"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ42904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="29782" checksum="EC991F242DC9677A" modified="2014-01-22" version="1">MTNAVVAGLNRGRIELRHLLTTPQDLWNSVFPSLIYLAVLFFTRGKTVPGTDFALASRTLPSLLGTIVVFTGVTTMMLALVVEREDGTLLRARTLPRGLAGYLVGKIFLAASVTLVNITVVLVPSLFLLDGLRTSSVSSWLTLLGVLVLGLLAALPAGAILGSLLNGVRTTGLVTAPLMGLVAISGIFYPITGFPEWLQAVAHVFPIYWLGLGMRSALLPDALAAAESEGSWQQLETAGVLGAWAVAGLIVAPIVLRRMARHESGSTVEARRDRAFGVTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1S7</accession>
    <name>U5W1S7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363639.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23905"/>
    <dbReference type="EnsemblBacteria" id="AGZ43049">
        <property type="protein sequence ID" value="AGZ43049"/>
        <property type="gene ID" value="AFR_23905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4842"/>
    <dbReference type="HOGENOM" id="CLU_1369666_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665450" evidence="2">
        <location>
            <begin position="30"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="199" mass="20622" checksum="D46366C192215044" modified="2014-01-22" version="1">MNETVTRRITPAGFLLALLLFLVLPLATASCDVPPDSASSGGTLGTSISGADLVVSHEHLDADGGLALSPAQQDRADLYVAVPRGVQILALLTVAALLAATATPLIRRVRVRAAVAAGAAVAALALVAVMAYLIRAKLTEAAEFFLLMGSYLPSVEGRDLDGRAGEVVHLGWGLWTTTAVLLLIVALQIMTLVRPPRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W238</accession>
    <name>U5W238_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyridoxal-5'-phosphate-dependent protein subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18640</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362379.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18640"/>
    <dbReference type="EnsemblBacteria" id="AGZ42006">
        <property type="protein sequence ID" value="AGZ42006"/>
        <property type="gene ID" value="AFR_18640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3783"/>
    <dbReference type="eggNOG" id="COG0498">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_0_11"/>
    <dbReference type="OrthoDB" id="428821at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="61"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="39057" checksum="DD1E623FA8C8AA7F" modified="2014-01-22" version="1">MLVDRAGRRYPLDEPRWQGDDGSPLLVEDQPGITRGDVDTRVRSQWRYAAALPLTYDRPVSLGEGCTPLLPLEVDGMTLAVKPEWFNPTASFKDRGTTVMVSTLARQGVTEMLEDSSGNGGSSVAAYAAAAGIRATILAPSSTSAAKIQQSRVHGATVELVPGTRQATADEAVRRSATTFYSSHNWHPFFLQGTKLIAYEIWEDLGFTAPDAVILPAGAGSLVLGCHVGFGELLRSGAITRRPRLLVSQPRNCSPLVSAFAAGAESVSPGEWTPTIAEGTAIAMPVRDREVLAAIRTSGGDMTAVDETDLGPATFELASRGLYAEPTSAAILPAIREFVRRGSLRPGETAVAVLTGTALKAADTIGRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W241</accession>
    <name>U5W241_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_23970</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23970"/>
    <dbReference type="EnsemblBacteria" id="AGZ43062">
        <property type="protein sequence ID" value="AGZ43062"/>
        <property type="gene ID" value="AFR_23970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4856"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="100"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="35847" checksum="C0AC8B045C7F4AE5" modified="2014-01-22" version="1">MFDTADTTAEKFTQMFAAILLFAAVVGIILFIAGRFGGRRGDRIVAYSYLAPTLIMLIVGLLYPGLRTIYESFFDAAGSAFIGIDNYKTIFTDSDQLTVLRNTVFWVVVTPFVATGVGLLYAILVDKAKLESFAKALIFLPMAISFVGASIIWKFVYEFRPDQANVKQIGLLNQVLVWLGGSPQQWLVDSPLNTFLLIVVMIWIQAGFAMTVLSAAIKAIPDDIIEAARLDGVGPWGMFRFVTLPAIRPAVVVVLTTIGIGTLKVFDIVRTMTGGQFDTSVIANEFYSQSFRSDNQGLGSALAVLLFVLVIPIVAYNIRQIRQSEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2N1</accession>
    <name>U5W2N1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YkuD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_25625</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25625"/>
    <dbReference type="EnsemblBacteria" id="AGZ43389">
        <property type="protein sequence ID" value="AGZ43389"/>
        <property type="gene ID" value="AFR_25625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5194"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056729_2_0_11"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665625" description="YkuD domain-containing protein" evidence="3">
        <location>
            <begin position="34"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="4">
        <location>
            <begin position="135"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="26736" checksum="B045B09B5BB7CB89" modified="2014-01-22" version="1">MQRVSARLAAAFVAVAVGGGLGAVALGPAAAGAAGRTVSATPITAVVVTKAAAASCSTGKYQKQVEGYLKKIGGYGTVTVDGKQSAADCKAIKKFQTRFGIKPAAGLAGPTTNGVAKRLAATKTSKCKAKSSGYTFCVDLTHQTTWVMKNKKVYLKPTVTRTGMKGYRTPSGTYKINKRTKKEWSDPYEVWLPYWQRFIGGRGFHQTTTYIHDAWRGSHGCVNLLPADAKAYYKIGKIGMRVSVIGRRSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2P6</accession>
    <name>U5W2P6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_26205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364267.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26205"/>
    <dbReference type="EnsemblBacteria" id="AGZ43503">
        <property type="protein sequence ID" value="AGZ43503"/>
        <property type="gene ID" value="AFR_26205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5311"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="10"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="26344" checksum="D013D7672C3A197B" modified="2014-01-22" version="1">MTITTIGTAVRLRAVTRIFGTGESAVTALDAVDVEIPPATFTAVMGPSGSGKSTLLHCAAGLDRATSGEVAIDGVSLTGLSERALTKVRRERVGFVFQAFNLIPALTAEQNVGLPLRLARRRPAPGQVEQMLAEVGLADRARHRPSELSGGQQQRVAIARALVSRPAVLFADEPTGALDTTASREVLRLLRTAVDRHAQTIVMVTHDPYAAAHADRVLFLADGRVVDALDNPSGASEIAVRMARLEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2P9</accession>
    <name>U5W2P9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25105"/>
    <dbReference type="EnsemblBacteria" id="AGZ43287">
        <property type="protein sequence ID" value="AGZ43287"/>
        <property type="gene ID" value="AFR_25105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5087"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="843"/>
            <end position="905"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="905" mass="96604" checksum="F32B9FE6E76FBC2B" modified="2014-01-22" version="1">MMLGRTAERALIDGLLTGARDGRSDALVITGEPGIGKTTLIGYAIENATGLRVLTARGWEDESEIPFAGLADLLRPVLDGLPGLPAPQAAALRSALALGPPVAGDRFTVCAATVGILAAAAETSPLLIVVDDLQWLDASSREAVLFAGRRLHADGIALLVATRNPADAADLRAQHVPLSGLSIEDVDELCRATLPAGPPGAAARLHAATAGNPLGVIELSREHPGTADDDGVTAPYVPPGSRIERALQARLAELPDATRRALVLAAAAVGGDTGILLLAAQATGLGLRDFAPAETQDLITIDDRRTEFRHPLLRSVVYHAATMDERCAAHLALATALANVPGDAAADARAWHLATATLSPDETVATTLQETALRASSRAGYVAAARAFGQAARLSLGPDRARRLLRAARCWQLAGRNDQVLPLLDEALPLTTDPSRRALVQHMSAYVRMWRERPDAVLEYLVAAAEAVEEADPGRAALMYSDACIPYFMVGDIEQLQAVVRRGHELASRTGGAPQLVAAVALAGGLTLAGERQAAAELLLAGHAELRLADPLVRAQDLCHAALTWIWLEDYDRAGELLDRVVTAARAAGALGVLPQALGIESELHFRTGRWHDARVSAAESLRLASETRQADLYGLFFAGRLDAVQGRPDDCRRRVERTMSVAGRLGVDCMALYTGHELGLLALSEGDTSTAIARLEEVRALPVVAHIHDPAVVPWVFDLVEAYIRDGRTDAARELLDELEDRTGGLWAAAAAARCRALLAGPEELDDAFRIALESPGCALMPFERARTQLCLGERLRRHRQRAQARKHLHDALETFTRLGAEPWADRARSELRATGSTIGRSADALPRLTPQELQVALVVGRGATNHEAAATLFLSRKTIEYHLSNIYRKTNIRSRTDLAGIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2V2</accession>
    <name>U5W2V2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_25975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364181.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25975"/>
    <dbReference type="EnsemblBacteria" id="AGZ43459">
        <property type="protein sequence ID" value="AGZ43459"/>
        <property type="gene ID" value="AFR_25975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5266"/>
    <dbReference type="eggNOG" id="COG2516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041526_0_0_11"/>
    <dbReference type="OrthoDB" id="660510at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016779">
        <property type="entry name" value="rSAM_MSMEG0568"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01107">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04043">
        <property type="entry name" value="rSAM_MSMEG_0568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="94"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="350" mass="37082" checksum="D4CAD38E685703F5" modified="2014-01-22" version="1">MTLGFRAELAVRGVRVATPVRRPAGAGPSDDGHLVINGANAALPINPDSPYEIRDDKVWLGETDTGLSLQVVRRPQFYDLTTADGVRYEQIARLHGRDVLATTVVQTCIRYAEEQRCRFCTIEESLRSGSTIAAKTPAQLAEVAEAAVRLDGVKQMVMTTGTTTGPDRGARNLVRSVRAVLEAVPGLPIQVQIEPPGDLDVIDDLHAAGASSIGIHVEALDDTIRRKWMPGKGSVPMAEYEAAWDRAVAVFGRNQVSTYLLIGLGEDPDDLVEGAGKLIARGVYPFVVPFRPMTGTLARRDGIPGPAPSLVRDISARVAALLREAGMLGADQKAGCAACGACGVLQAAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W397</accession>
    <name>U5W397_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LuxR family two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_26560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26560"/>
    <dbReference type="EnsemblBacteria" id="AGZ43574">
        <property type="protein sequence ID" value="AGZ43574"/>
        <property type="gene ID" value="AFR_26560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5384"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="4"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="146"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="216" mass="22773" checksum="E677EAD58285CC26" modified="2014-01-22" version="1">MTIRVVIAEDAVLFRAGLARLIEDEGHQVCAAVGDGGALLEAVAEFQPDVVVADIRMPPTHTDEGLRAALEIRTRQPRTGVLVLSQYIETKYTARLLEGNAAGAGYLLKERVADVADFADALGRVAAGGTALDPEVVGQLLRVSRHAGGVAALTARERQVLALMAEGRSNAGIAGTLVVTGGTVEKHVASIFDKLGLPQDEADNRRVLAVLRYLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3A8</accession>
    <name>U5W3A8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26635"/>
    <dbReference type="EnsemblBacteria" id="AGZ43589">
        <property type="protein sequence ID" value="AGZ43589"/>
        <property type="gene ID" value="AFR_26635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5398"/>
    <dbReference type="eggNOG" id="COG5032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085318_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022292">
        <property type="entry name" value="CHP03843"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03843">
        <property type="entry name" value="TIGR03843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="29043" checksum="E89B856620E3DCF9" modified="2014-01-22" version="1">MLAEDEALALLSRGRMELEGRLVDASNTTLRAEITLDGLTRRCVYKPVQGERPLWDFPDGTLAGREVSAFLVSQATGWGLVPPTILRDGPLGPGACQLWIDEPEDAESLIGFVPAYDVPEGWLPIAQARDEDGDAYALAHADDPRLSRLAVLDAVINNADRKGGHVLYAPDGRIHGVDHGVSFHVENKLRTVLWGWTGKTIPAESIEVLNRVGELLDGTLGEALEEHLTVSEVQHARLRVRRLLRSTRFPKPPQDWPAVPWPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3P9</accession>
    <name>U5W3P9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH-type transcriptional repressor yvoA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362913.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21355"/>
    <dbReference type="EnsemblBacteria" id="AGZ42541">
        <property type="protein sequence ID" value="AGZ42541"/>
        <property type="gene ID" value="AFR_21355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4332"/>
    <dbReference type="eggNOG" id="COG1725">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_10_1_11"/>
    <dbReference type="OrthoDB" id="1860081at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12397" checksum="EB92B69FC2FADABA" modified="2014-01-22" version="1">MDIVVDADSAVPPYEQVRLRIAELAADGTLAAGTRLPPVRQLATDLGLAANTVARAYRELEQAGLVETRGRAGTLITARAGRTPAEAQQAAQRYADRATALGLTPQAALDLVRAALHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Y4</accession>
    <name>U5W3Y4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27915"/>
    <dbReference type="MEROPS" id="S12.950"/>
    <dbReference type="EnsemblBacteria" id="AGZ43844">
        <property type="protein sequence ID" value="AGZ43844"/>
        <property type="gene ID" value="AFR_27915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5657"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_1_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="2">
        <location>
            <begin position="86"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="439" mass="45862" checksum="3F96E07923B0B58D" modified="2014-01-22" version="1">MLLGAGFAATVAGCGSGDSSPQAGPTWAEPAAPPEPALAGGTPTRPAPAVTDRPAGEAVVAATYGDKVKSVLAKYLKPTSDNPRHPGYAGAVALVMVDGRTTVHTAAGHALRYKAGPVELDASKRVTMKTDAIFDVASLTKVYTAILVLQLVDRGKVELDAPVQKYLPEFTGTGKGAITIAMLLAHTSALPVGAKVTGLPDNTARWKSVLSTPLVKGGVPGTTFRYSSVGLMVLGRLVEKLTGKSLDKALRDNLTTPLGLKDTGFLPLKWVGAKNRLVATDARSSRGLLRGTVHDDVCNHLGGVAGHAGIFATARDVAVIGQMLLNGGTHNGKRILTSATVRKMLTNVNKGKPAIDPERPKRTADHGLGVEMNQSWFMGKLSTAATFGHTGFTGTSLLVEPRRKLVLVLLTNRAHPNWSWSDPDTHRVAVANAVAGGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4H6</accession>
    <name>U5W4H6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alpha-n-arabinofuranosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_29185</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29185"/>
    <dbReference type="EnsemblBacteria" id="AGZ44098">
        <property type="protein sequence ID" value="AGZ44098"/>
        <property type="gene ID" value="AFR_29185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5913"/>
    <dbReference type="eggNOG" id="COG3507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009397_11_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006584">
        <property type="entry name" value="Cellulose-bd_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003305">
        <property type="entry name" value="CenC_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02018">
        <property type="entry name" value="CBM_4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03422">
        <property type="entry name" value="CBM_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00606">
        <property type="entry name" value="CBD_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666353" evidence="4">
        <location>
            <begin position="41"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="5">
        <location>
            <begin position="342"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="3">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="616" mass="64895" checksum="19693298DE65D66F" modified="2014-01-22" version="1">MHPHPIGRPALARPRRAVAVIAALLLAVTLMPLLGGPARADNPIVQTIYTADPAPLVHNGRVYLYTGRDEDNSTYFTMKEWRVWSSADMVNWTDHGSPLNLASFSWASSNAWAGQAVERNGKFYWYVPMTARATGRMSIGVAVADSPTGPFRDALGHPLVENNEIDPTVFIDSDGQAYLYWGNPNLTYVKLNADMISYSGSPTRIPLTTAGFGTRTGDANRPTLYEEGPWVYKRGGTYYMVYAAKCCSEFIAYSTAPGPLGPWTYRGTVMPTQGGSFTNHPGIVDFNGSSYFFYHNGALPGGGGFTRSVAVEKFSYNADGSIPTITMTTAGAPQVGTLNPFVRQEAETIATEAGVETEASSEGGMNVANIENGDHVRVKGVAFGSGATSFTARVASATSGGRIEVRTGSPTGALAGTCTVPGTGGWQAWTSVSCPVSGLSGTKDLYLRFTGGSGFLLNVNWWQFTGAATGGNLLTNAGMEDGTSGWLVNGSGTLSTVTDVVRSGSRALAGTGRTGAWNGPGQDVTSRMTNGKSYSTSVWVRTPSGSPAAKATLALTANGTTSYLQLTPTATVNASGWTQVSGTATVSWSGTLTRAIFYVETAAGTDNLYLDDASFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4J4</accession>
    <name>U5W4J4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363442.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23225"/>
    <dbReference type="EnsemblBacteria" id="AGZ42915">
        <property type="protein sequence ID" value="AGZ42915"/>
        <property type="gene ID" value="AFR_23225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4707"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_0_2_11"/>
    <dbReference type="OrthoDB" id="1167152at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044999">
        <property type="entry name" value="CbbY-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896">
        <property type="entry name" value="PTHR42896"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="255" mass="27093" checksum="7C4BD346414B026E" modified="2014-01-22" version="1">MTALIFDCDGVLADTERDGHLPAFNSTFEQFGLPVRWSEDDYAEKLKIGGGKERMASLFADPAFAAAAGDGDRAELLLTWHRAKTATFKQLVALGKIPARPGVARIVRAALDTGWTVAVASTSAEDSVRAVLTNAVGATTAARIPVFAGDVVPAKKPDPAIYRLTVDRLGLDPADTLVIEDSRNGLLAATGAGLRCLVTVSSYTRDEDFGEAALVVSELGDPGRPPIEVLANRSSARPEAYLTLDDLRACLEEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4S2</accession>
    <name>U5W4S2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560535.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44198">
        <property type="protein sequence ID" value="AGZ44198"/>
        <property type="gene ID" value="AFR_29685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6017"/>
    <dbReference type="eggNOG" id="ENOG5031YIV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1131705_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="27680" checksum="36F09899643ECE8B" modified="2014-01-22" version="1">MLIHAATAEAPSWTDKLEAWSTFGAAIFTATAVVVAFLVWRHDQRLRREDKQDADAAQARLVFVTMIQALGSKEEGWLGVKVAIRNNSPGSISSVRLKAEAAASSTLIRLVRAIGPGEAPQQELMFTTPRPWPAATVRPSSEKFRRRVRCRLSFHDSAGLSWTRWDRDEPFRGSSTVSTQLRVLPLLAEYLRLMEPIEWIKTRIWKLHTLAAMALQHKINARWELDMDDEELSSAKPEQIQAPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W500</accession>
    <name>U5W500_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363611.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23765"/>
    <dbReference type="EnsemblBacteria" id="AGZ43021">
        <property type="protein sequence ID" value="AGZ43021"/>
        <property type="gene ID" value="AFR_23765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4814"/>
    <dbReference type="eggNOG" id="ENOG502Z7YZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071811_0_0_11"/>
    <dbReference type="OrthoDB" id="1520004at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="27080" checksum="EE0D0CF96BAE6D2F" modified="2014-01-22" version="1">MSKLNQIIAVEKGVKSKAFADLSEAHHAVQKTGPLAGISRAYQPKDEEGEQLPAESTRVQIKAEDILREVAGTLTRLFDVTATKDWTNCVARADVTVDGRTIATQVPVTYLLFLEKQLVDLHTFVKKLPVLDAAESWVRDDSTDSWRTEPVRTNRTKKVPRNHVKAEATDKHPAQVEVYYEDITVGYWTTVKFSGALPAKRVNEILERVLALQTAVKFAREEANNTEVADQRVGAAVFGYLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W503</accession>
    <name>U5W503_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23885"/>
    <dbReference type="EnsemblBacteria" id="AGZ43045">
        <property type="protein sequence ID" value="AGZ43045"/>
        <property type="gene ID" value="AFR_23885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4838"/>
    <dbReference type="eggNOG" id="ENOG5031VK5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691746_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="17890" checksum="8D9F2665E2417755" modified="2014-01-22" version="1">MRGMAEPAVPTTAPRLELPLAEARTRFVQLARLAGLTRQTTVVTDKGRPLAAIVPIPAPAAPSPSARAPGPTGASGLVGVPHRAGAASPARSLAPAAEPARERASEQSAAGWIRRIETLRADLRRQHAGLEQALDQAWHELDKLSPPGTDAQLDALRTAHADLRTGRTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5S3</accession>
    <name>U5W5S3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histidine triad (Hit) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31145"/>
    <dbReference type="EnsemblBacteria" id="AGZ44489">
        <property type="protein sequence ID" value="AGZ44489"/>
        <property type="gene ID" value="AFR_31145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6304"/>
    <dbReference type="eggNOG" id="COG0537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="39"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="3">
        <location>
            <begin position="133"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="177" mass="19361" checksum="0B55D13236505106" modified="2014-01-22" version="1">MSDVGEPDGLDRLWTPHRMAYISGEDRPEGGYEKPGGCPFCVAPGLPEGESLVVARGELVFAVLNLYPYNPGHLLVCPYRHVADYTDLTEEETVEVAAFTRRAMRVIRQVSSPHGFNLGMNQGAVAGAGIAAHLHQHVVPRWGGDGNFMPVIGRTKVLPQLLGDTRDLLRKSWSETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5X7</accession>
    <name>U5W5X7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25705"/>
    <dbReference type="EnsemblBacteria" id="AGZ43405">
        <property type="protein sequence ID" value="AGZ43405"/>
        <property type="gene ID" value="AFR_25705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5210"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106319_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="22893" checksum="C42B5306D7E125F7" modified="2014-01-22" version="1">MNDWPLFGLRMRCRDVTLRPVREVDLPVLADLLPPDYDQDPRLESFPGLSVAADQRRLFCQGYWRALGTWSPSSWVLHLAVEVSGRPVGVQTLEGDDFPALRTVDTASWLTPSVRGRGFGVAMRTAALGFAFSTLEAVAAVSSAVPENAASLGVSRRLGYEDNGVGLVSAPGGRVVELRHLRLTAETWRSARHEVTVTGFEGCRPWFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6X2</accession>
    <name>U5W6X2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560014.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27075"/>
    <dbReference type="EnsemblBacteria" id="AGZ43676">
        <property type="protein sequence ID" value="AGZ43676"/>
        <property type="gene ID" value="AFR_27075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5487"/>
    <dbReference type="eggNOG" id="ENOG5031WKI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_902585_0_0_11"/>
    <dbReference type="OrthoDB" id="2131458at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="31941" checksum="807B407EC274814A" modified="2014-01-22" version="1">MPNDLESAFAALSDDAARARLAPAASLRRRSDRRVVTRSLAGVAAAAVLVAGVTVGARLVLADEALPPLPPAQSTAPTVAPSPAPSLSPSATPSSPPPSSPSSPPPSSPPETEEPSIPKSIPARAFLTRSDANVAQLYRSDSAHGSPDLCSAASYPSDSKDGVRGTSNIVYRNPGDGEDYTPVGQVADTVVVYRGDGAEEFMDELRAAVRNCPEGKRDNLTYKYRSLGSVGVGDESVLIQGSTPARGDNGELESDGSTYKIYFAAVRVGDSVALVENTGYESISAERPVAESFARKAAQRLENWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W774</accession>
    <name>U5W774_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">L-carnitine dehydratase/bile acid-inducible protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560177.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27895"/>
    <dbReference type="EnsemblBacteria" id="AGZ43840">
        <property type="protein sequence ID" value="AGZ43840"/>
        <property type="gene ID" value="AFR_27895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5653"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_5_0_11"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="38625" checksum="713D6F27EEA1B046" modified="2014-01-22" version="1">MTGAQGPLRGLKVVELAGIGPAPFAGMLLADLGADVVRVDRPGPGSPLAGDPRLDLLNRGKRSITADLKQDSGRELVLALASRAAVLIEGYRPGVTERLGIGPAQVLARNPALVYGRMTGWGQDGPLAGSAGHDITYLAITGALHAIGRADGPPQVPANLLGDFGGGALYLVVGLLAALREADRTGRGQVVDAAITDGVTHLTTLLYGLSAEGRWEDTRGVNLLDTGAPFYDVYPASDGGHLAVGALEPQFYAEFVRLLDPGDNLPAQYDRARWPELRTRLAATFATRTRDEWAVIFEGTEACVAPVLSFAEAARHPHLVHRATLADRDGVRQPAPAPRFSATPPGPVGAPALPGEHAASILADWEISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7D9</accession>
    <name>U5W7D9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_28025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28025"/>
    <dbReference type="EnsemblBacteria" id="AGZ43866">
        <property type="protein sequence ID" value="AGZ43866"/>
        <property type="gene ID" value="AFR_28025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28025"/>
    <dbReference type="eggNOG" id="COG3226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_21_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041583">
        <property type="entry name" value="TetR_C_31"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17940">
        <property type="entry name" value="TetR_C_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="22023" checksum="493A4B4E555C1EDA" modified="2014-01-22" version="1">MTRFIVITDAAVAVVAELGMRGLTHRAVDSRAGLPPGTTSAYFRTRKALIEALVQRLADLDRADLERAELEGQRLPADPDALAASIAAVIDSWLTTGRDRTLARYACLLEATHHPELRTILGHGESSRVQARALLAAAGAAHPDQAGDHFVACVDGLLFDRLIGAGALTAPTPGTPQSRADLATAVRAVLRGLSEQPGPPAPPGDPQPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7I4</accession>
    <name>U5W7I4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_34450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561485.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34450"/>
    <dbReference type="EnsemblBacteria" id="AGZ45148">
        <property type="protein sequence ID" value="AGZ45148"/>
        <property type="gene ID" value="AFR_34450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6970"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="23858" checksum="9C2CE37D5CBA5D12" modified="2014-01-22" version="1">MSDPLIEVDGVGRDYGTGDRTVHAVREVSFEAARGELVAVRGRSGAGKTTLLNMIGGLDRPTRGVVRVAGVDVTSANEAQLLDLRRSTIGFVFQSFGLIPILSAAENVSVPLRLNRRPAAEREERVQLLLELVGLGGQAAQRPGELSGGQQQRVAVARALANDPPLLIADEPTGQLDSETGRSIMDLLRALVDARGMTALVATHDPSLIDRADRVLTLRDGRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7M7</accession>
    <name>U5W7M7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclopropane fatty acid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFA/CMAS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561349.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33770"/>
    <dbReference type="EnsemblBacteria" id="AGZ45012">
        <property type="protein sequence ID" value="AGZ45012"/>
        <property type="gene ID" value="AFR_33770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6836"/>
    <dbReference type="eggNOG" id="COG2230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026434_6_2_11"/>
    <dbReference type="OrthoDB" id="1518440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003333">
        <property type="entry name" value="Mycolic_cyclopropane_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003085">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="423" mass="45644" checksum="84D9D609DF180C3A" modified="2014-01-22" version="1">MTALAVESGIAGQLAALVSPLAGGALPVRLRAWDGSESGPAGGPVLVIRDRKALRRLMWSPDELGLAQAYIAGEIDVEGDLGAGLRQVWQQARARGLARGGVRVGLGDKLRAVRTLLRLGVLGPKPPAPDVQARIVGDLHSRERDRAAISYHYDLSNEFYALILDERMAYSCGYWTSDAPGYGLAEAQTDKLDLVCRKLGLEPGMRLLDVGCGWGALGLHAAKNYGVTVTGVTLSREQLAFAQARVGGLPVDFRLQDYRDVADGPYDAISTIEMGEHVGQGQYPVFAAQLHRLLRPEGRLLVQQMSRGATAPGGGAFIESYVAPDMHMRPVGQTVDLLEQAGLEVRDVHALREHYVRTVGVWLETLEARWDAVVALVGEPMARVWRLYLVGGALTFEEGRMGVDQILAVRPTETGVSGMPATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Q9</accession>
    <name>U5W7Q9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28625</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560323.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28625"/>
    <dbReference type="EnsemblBacteria" id="AGZ43986">
        <property type="protein sequence ID" value="AGZ43986"/>
        <property type="gene ID" value="AFR_28625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5804"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="24177" checksum="3D29C9F6E1EB089D" modified="2014-01-22" version="1">MRVLVADDERLLAETIAVGLRRAAMAVDVAYDGEQAIEKIQINRYDVAVLDRDMPGGTGDDVCRWIGEHQVGTRVLLLTAACGVYDRVEGLGLGADDYLTKPFAFAELIARVQVLARRTAPALPPVLQRDGVVLEVATRTASRAGRPLALTHKEFGVLHTLMRAQGQAVTTEDLLEQVWDEHTDPFTNVVRVTVSTLRRKLGEPQVVQTVPRAGYRLGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W833</accession>
    <name>U5W833_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560467.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29345"/>
    <dbReference type="EnsemblBacteria" id="AGZ44130">
        <property type="protein sequence ID" value="AGZ44130"/>
        <property type="gene ID" value="AFR_29345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5947"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096009_0_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="178" mass="19211" checksum="0B3B3EB397D821D3" modified="2014-01-22" version="1">MPAAGTTTKSDERRQHVVDAAVSCFARRGFYGTTTNEIAERAGISQPYLYRLFANKQAIFAAAVTHVGGLLTETLTAAKAADTTMSPPQALRAAYSTLITDGDVLRFLMHANCATDEPVIRDAVRTCYAQQVTIVRKLLDGDDEAVRQWFAAGMLDNVITMLGLADVAEPWAATLIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W889</accession>
    <name>U5W889_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YuaI-like N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561764.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35860"/>
    <dbReference type="EnsemblBacteria" id="AGZ45428">
        <property type="protein sequence ID" value="AGZ45428"/>
        <property type="gene ID" value="AFR_35860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7258"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_18_2_11"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="19291" checksum="4FE83AD23E1480ED" modified="2014-01-22" version="1">MVDIRPLTDADIDGVAVVHVRSWQVAYAGIVPAEHLDRMDPAVNAERRRTRAEIPGSRTLVADDNGTVVGFVSFGPYRVDQGDGVNPALGELYAIYVHPDRWHAGIGRRLLRAARAELTAAGYPVMRLWVLEDNHRSRRFYSRAGLAPDGERSIYTPRGTTVELPEIRYSARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8G9</accession>
    <name>U5W8G9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpsO</name>
        <name evidence="7" type="ORF">AFR_35915</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="3 6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561775.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35915"/>
    <dbReference type="EnsemblBacteria" id="AGZ45439">
        <property type="protein sequence ID" value="AGZ45439"/>
        <property type="gene ID" value="AFR_35915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7270"/>
    <dbReference type="eggNOG" id="COG0184">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148518_0_0_11"/>
    <dbReference type="OrthoDB" id="1990141at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_S15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_S15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="PTHR23321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="89" mass="10460" checksum="ABD5283874C1DE92" modified="2014-01-22" version="1">MALDQETKTKIMTEYATLEGDTGSPEVQVAMLTKRIADLTEHLKVHKHDHHSRRGLLLLVGRRRRLLNYVQKKDIARYRTLIERLGLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8J7</accession>
    <name>U5W8J7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29950"/>
    <dbReference type="EnsemblBacteria" id="AGZ44251">
        <property type="protein sequence ID" value="AGZ44251"/>
        <property type="gene ID" value="AFR_29950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6066"/>
    <dbReference type="eggNOG" id="ENOG5030F1S">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056295_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="119" mass="12384" checksum="838911CE7D2D1412" modified="2014-01-22" version="1">MTYPNQQPPVNPRPGPPSGGFRPGPPPGGFRPGPPQPQAQPQAPAYRPEPQHSPQQPQQYSPQQPPAVSPPDGGGTGHPAVDAVLQSLANAAALAPADQIAEYEAAHQVLQETLASIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8K8</accession>
    <name>U5W8K8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36115"/>
    <dbReference type="EnsemblBacteria" id="AGZ45479">
        <property type="protein sequence ID" value="AGZ45479"/>
        <property type="gene ID" value="AFR_36115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7309"/>
    <dbReference type="eggNOG" id="COG1511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004534_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017501">
        <property type="entry name" value="Phage_infect_YhgE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017500">
        <property type="entry name" value="Phage_infect_YhgE_N"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03062">
        <property type="entry name" value="pip_yhgE_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03061">
        <property type="entry name" value="pip_yhgE_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="560"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="591"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="621"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="675"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="714" mass="73472" checksum="4A75D84B74DF2909" modified="2014-01-22" version="1">MTGLSSFRLAGFELRRFLRGRLPVAALVVLAVIPLLYGALYLYAFWDPYGRLNHIPAALVVEDVAATASDGTKVDAGHDLADELIKREVFDWHVTDERGAEAGLADGSYQLSLRIPKDFSTDLTTGPDANATPKSAQLVVINDDATNYLSGVFSRTAFQEVQAAAAQTAQAGYFDKMLIGFTDLKSQTQEAADGATKIDNGLTDANQGAGELEDGLTDADRGAGKLSGGLGTAAQGADDLASGLATLQSGAAQLATGTQQAAAGGKQLATTVDAAADRIGPVLRDNAELIEDAANQVADGADLLARNVGRLSSAADDAVEQAEDLQAYLNGLPDETEGIDQARAAAKELVTAAKRVQSTIRAADLDALSDQLHEVAATAHKVARAAPHLADDVAAARRNVDDLAAGLGDLATGAKQLNSGAADAVAGATDLRGGIYQLASGARSLDNGLDRLTTGGQQLADGLGDLQSGAAKLSSGLSDGAKQIPGYGDDPQARADLLSDPVALDRTVRHPAATYGVGFAPYFLALALWVGAMITFMVLRPLNRRYVVSGAPAYRVTLAGLLPAVAIGLAQATLLFVVVVFGLGLGPVNPVVTLGLLMLTATAFAAIMQLLGAALGPAGRIVALALLMLQLTSSGGTYPVQTTPGFFQVIHPLLPMTYVVDVLRHAIDGGPAGTVVTGMLALLGYGLGALALTVAVARGKRRLTPSDLHPELVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8N9</accession>
    <name>U5W8N9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="11">ATP-PRT</shortName>
            <shortName evidence="11">ATP-PRTase</shortName>
            <ecNumber evidence="4 11">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">hisG</name>
        <name evidence="14" type="ORF">AFR_30475</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Feedback inhibited by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ATP phosphoribosyltransferase family. Long subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="4 11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560692.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30475"/>
    <dbReference type="EnsemblBacteria" id="AGZ44355">
        <property type="protein sequence ID" value="AGZ44355"/>
        <property type="gene ID" value="AFR_30475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6170"/>
    <dbReference type="eggNOG" id="COG0040">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038115_1_1_11"/>
    <dbReference type="OrthoDB" id="1419568at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP_PRibTrfase_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HisG" evidence="12">
        <location>
            <begin position="49"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="HisG_C" evidence="13">
        <location>
            <begin position="206"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ44355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="30134" checksum="4172D41CF3624645" modified="2014-01-22" version="1">MLRIAIPNKGTLSAPASQMLKDAGYRQRTDPKDLLCRDEANNVEFFYLRPRDIATYVGSGDLDLGITGRDLLVDSGVPAQEVLDLAFGGATFRWAAPAGTLTSADEIGGRRIATAYPGVVERYLSEHKLEADVVRLDGAVENAVRLGVADLIADVVETGTTLRQAGLVTLGDPILRSSAILIGREAAGETGPAAQLVRRLQGVLVARSFVMLAYDVRADLLDQATALTPGIESPTVSPLHREGWVAVQAMVRRSDTHKIMDALYDLGARAILVTDISNCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8P3</accession>
    <name>U5W8P3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 13 15">tRNA (guanine-N(1)-)-methyltransferase</fullName>
            <ecNumber evidence="6 13 15">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 17" type="primary">trmD</name>
        <name evidence="17" type="ORF">AFR_36315</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13 15">Specifically methylates guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13 15">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 13 15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13 15">Belongs to the RNA methyltransferase TrmD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="6 13 15"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561855.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36315"/>
    <dbReference type="EnsemblBacteria" id="AGZ45519">
        <property type="protein sequence ID" value="AGZ45519"/>
        <property type="gene ID" value="AFR_36315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7349"/>
    <dbReference type="eggNOG" id="COG0336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047363_0_0_11"/>
    <dbReference type="OrthoDB" id="525632at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N(1))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18080">
        <property type="entry name" value="TrmD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00605">
        <property type="entry name" value="TrmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002649">
        <property type="entry name" value="tRNA_m1G_MeTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023148">
        <property type="entry name" value="tRNA_m1G_MeTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016009">
        <property type="entry name" value="tRNA_MeTrfase_TRMD/TRM10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46417">
        <property type="entry name" value="PTHR46417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01746">
        <property type="entry name" value="tRNA_m1G_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000386">
        <property type="entry name" value="tRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00088">
        <property type="entry name" value="trmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 13 15" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA_m1G_MT" evidence="16">
        <location>
            <begin position="22"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="13 14">
        <location>
            <begin position="133"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ45519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="254" mass="27601" checksum="A2530F22BD4E39B1" modified="2014-01-22" version="1">MRVDIVSIFPDYFAPLDLSLIGKARGSGLLDLAVHDLRTWTSDVHRTVDDSPYGGGPGMVMRPEPWAAAFDAVARPESVLVVPSPVGKPFTQAAAHELAALPHLIFACGRYEGIDQRVLEDAADRMPVREVSLGDYVLFGGEVAVIVIMEAITRLLPGVLGNADSLTEESHAAGLLEAPVYTKPAVWRDRAVPEVLRSGDHGRIARWRRTESLVRTATRRPDMFAAYPGEQLDKKDRAALDEAGFQITPPGVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W931</accession>
    <name>U5W931_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Laminin subunit alpha-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562033.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37220"/>
    <dbReference type="EnsemblBacteria" id="AGZ45698">
        <property type="protein sequence ID" value="AGZ45698"/>
        <property type="gene ID" value="AFR_37220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7530"/>
    <dbReference type="eggNOG" id="COG3599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017257_1_0_11"/>
    <dbReference type="OrthoDB" id="937807at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019933">
        <property type="entry name" value="DivIVA_domain"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03544">
        <property type="entry name" value="DivI1A_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="333"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="401"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="289"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="432" mass="47936" checksum="7FDE2E621FB4AF1E" modified="2014-01-22" version="1">MPQQQDANLAFFESANSQHDFTVVLRGYDRGQVDAHLGRLVAALNQSEQARGEAEQRMNDAQRRLRQAEQRLNALEQKLADSNKLLEENNRPTLSGLGTRVEQILRLAEEQANDHRSEAKRESEGILSAARLEAREITDKARAEAAAMKATAEREAGQVRTQAERESAENRVQARREADTLRSDADRETKQLRTVTAHEVAELKSTVEREVASLRATAEREITQLRAKAAREAEEKRAEATKMLTDARDKRDKDLQALALETAERREKSEREESQRHAAQVNATQKMVAEAEERARAAEDRAKEIEQRAESRRVESERTAIETVEKARALAEKTVTEARSESSRLLSEARTEAELTTQAAKREVDDLTRQKDAVTNQLGQMLSGLSGLVPTVGAAAKAAEVVKQAEAKSDAPAPREPEAEEAGQPEAVAKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W985</accession>
    <name>U5W985_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560839.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31230"/>
    <dbReference type="EnsemblBacteria" id="AGZ44506">
        <property type="protein sequence ID" value="AGZ44506"/>
        <property type="gene ID" value="AFR_31230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6321"/>
    <dbReference type="eggNOG" id="ENOG5032SHV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106273_0_1_11"/>
    <dbReference type="OrthoDB" id="2060858at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009937">
        <property type="entry name" value="Phage_holin_3_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07332">
        <property type="entry name" value="Phage_holin_3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="14850" checksum="E02B9F65E1F23C33" modified="2014-01-22" version="1">MADVLNGSPVRPATDQSTSELVQKASEQLSRLVKDEIALAKAELTEKGKHAGIGVGLFGGGGVLALYGVGALFATLIIVLDLFLPLWLAALIITVVLFATAGVLALLGKKQVTQAVPPEPSAAIASAKADVDEVKHAIKTRSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9G2</accession>
    <name>U5W9G2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_37675</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37675"/>
    <dbReference type="EnsemblBacteria" id="AGZ45789">
        <property type="protein sequence ID" value="AGZ45789"/>
        <property type="gene ID" value="AFR_37675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7619"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_2_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="511"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="54"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="557" mass="57731" checksum="85ECADF83CD291CA" modified="2014-01-22" version="1">MSPEKETGTTATRAEAVVAEDAVALAGVPVPDDAVAGEATAVAGRMSHREVIQALSGLMLGMFVSILASTIVSNALPRIIADLGGTQSVYTWIVTTELLAMTATVPLWGKMADLYSKKLLIQLSLGLFVAGSLIAGFTPNVEILIVSRVVQGIGAGGMTALAMIVMAAMIPPRELGRYSGMFGAVFGVATIAGPLIGGVLVDTSWLGWRWCFFIGVPFSVAAILLLQRTLHLPTVRREAVIDWLGAFLITAGVSDLLIWSTLAGNHFAWASWETAVFVAAGVILLALAVWAESRAPEPIIPLSIFRNRTVSLTIVASVLVGVAMFGGTVFLSQYFQVSLGKSPTVAGLMSLPMIFGLLVSSTVAGALITKYGRWKAYLVAGSIVMTGGMLLLSTISATTSALMLSIYMAVLGIGVGMLMQNLVLAAQNDVPANELGASTSALTFFRSMGGAIGVSALGAVLAARVTTLMTERFGPAATGGSGTAAVPDLATLPAPVRAIVADVYGQATAELFLVGAPIAALAVIAVLFIKEKPLHTMSGDERRAQEEKAAATGVPMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9L3</accession>
    <name>U5W9L3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_37290</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562047.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37290"/>
    <dbReference type="EnsemblBacteria" id="AGZ45712">
        <property type="protein sequence ID" value="AGZ45712"/>
        <property type="gene ID" value="AFR_37290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7544"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_4_0_11"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="9">
        <location>
            <begin position="14"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28490" checksum="CE8DFDCD613EAA19" modified="2014-01-22" version="1">MSAAKLGLGQGGLEVKQFLRSREQVMFTLAFPAILLLVFGSIFKGDIGQGVKFTQYFVTGMIATGLMTAGFQALAIQIPMERDRGVLKRLLGTPMPKWVYFAGKVLMVAFIAVLETAILLAVATLLFGLDLPDTGAKWLTFLWVSALGITACTLCGVAFSSLARTGRSAPAVVTPVALILQFTSGVFFVFTDLPAWMQNLASLFPLKWMCQGLRSVFLPESFGAREPGGSYDLPMVALVLGVWCLVALVLCLTTFRWTTKRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9N1</accession>
    <name>U5W9N1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pentachlorophenol 4-monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561018.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32135"/>
    <dbReference type="EnsemblBacteria" id="AGZ44685">
        <property type="protein sequence ID" value="AGZ44685"/>
        <property type="gene ID" value="AFR_32135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6506"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_20_1_11"/>
    <dbReference type="OrthoDB" id="867226at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="7"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="469" mass="49940" checksum="D5EE71D049894994" modified="2014-01-22" version="1">MGEPVHDVVVVGAGPVGLLLAIELETAGVKPLVLERLAEPDRGIKAAAVGAVGAEALERRGFLPALDGEQVTMLMKKPGGGHFSALFVVDQELQDDPGRHSRVVPQDALERILTVRAGERGIEIRRGVTVESFEQDPGGVSVQTSAGPLRTRYLVGCDGGRSRVRKLAGFAFPGTDPTITGYQALVEFDAPGKLTQGWQRTPRGLITSIPGRVFTAEFDGPPPDRDAPVTRAEVEASIQRVSGTDVRVLSMTKASRWTDHARQATTYRLGRVLLAGDAAHVHSPFGGQGLNLGLVDAANLGWKLAAAVRGDDRLLDSYTAERHPVGARVLENTRAQVALVRPDPLTTALRSIVSELIRLPDGNRLIGGMLSGTGIRYDLGDDDPLVGTLAKDQLLTLADGTEGRLYALMENGGGLLVSPSERLLPPGVQQAQTKEGPSLLVRPDGCIAWTETSSTSLDEALARWFPRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9V7</accession>
    <name>U5W9V7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44701">
        <property type="protein sequence ID" value="AGZ44701"/>
        <property type="gene ID" value="AFR_32215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32215"/>
    <dbReference type="HOGENOM" id="CLU_2679341_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="74" mass="8175" checksum="130B1524A20D44A5" modified="2014-01-22" version="1">MLHRPFDRLPFRGEDREGPERRAARVVGGVAEEGDRGFEARVGGPADQGVGFRWAFDQDEVGALAIELLDHRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9W3</accession>
    <name>U5W9W3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45993">
        <property type="protein sequence ID" value="AGZ45993"/>
        <property type="gene ID" value="AFR_38695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7829"/>
    <dbReference type="eggNOG" id="ENOG50327ET">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1640168_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665701" evidence="1">
        <location>
            <begin position="31"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="16762" checksum="498E6BEA98C8C20F" modified="2014-01-22" version="1">MDRSKFAVGTAAMLTALLLAAASGTPSASAGETMTITGTRDKAHGYNQQTFGIAMGGKAPKGLYPGVVREMKLTLRNPYDFALSVKSLEGKVVSTSKRKCKPTASNLVAGDYSGKLPLVVPPRGKVDAKTIPLFMPPNASAACQGAVFTIELTGTATKARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA25</accession>
    <name>U5WA25_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative SsgA-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_32615</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell septum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SsgA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014446635.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32615"/>
    <dbReference type="EnsemblBacteria" id="AGZ44781">
        <property type="protein sequence ID" value="AGZ44781"/>
        <property type="gene ID" value="AFR_32615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6600"/>
    <dbReference type="eggNOG" id="ENOG5032RFA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126599_0_1_11"/>
    <dbReference type="OrthoDB" id="1627707at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006776">
        <property type="entry name" value="SsgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038658">
        <property type="entry name" value="SsgB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04686">
        <property type="entry name" value="SsgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0749">Sporulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="15561" checksum="26FEEF7CFB1433E8" modified="2014-01-22" version="1">MSTIRPTTVEVETSLRLVAPDATALPVRASLRYDPADPYAVHVLFHAESAGGEAVSWSFARELLVTGLDEPAGIGDVRVWPWATPRGDFVALALSSPDGNALFEVPRSVLVRFLRRTYVVVPRGRESDHLDVDAAVNRLLAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA97</accession>
    <name>U5WA97_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562286.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38490"/>
    <dbReference type="EnsemblBacteria" id="AGZ45952">
        <property type="protein sequence ID" value="AGZ45952"/>
        <property type="gene ID" value="AFR_38490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7787"/>
    <dbReference type="eggNOG" id="COG4312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066898_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010296">
        <property type="entry name" value="DUF899_thioredox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05988">
        <property type="entry name" value="DUF899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="28205" checksum="4FB5DF599829A1BE" modified="2014-01-22" version="1">MSENKLPKIVSRDEWTAARRELLTSEKEAVRAKDALNTRRRELPMVELDKDYRFEGPDGEVSLLDLFDGQRQLVVQHFMFPPEWEDGCASCTAAVDELSDGLLGHLRARSTVYAVVARAGLATLEKYRAKRGWTIPLYSSSGSDFNYDFNVTIDASKAPVQFNFRGPDELRAAGFDWMLETEDQPMEQPGLSCFLRDGDRIFHTYSTFARGTEQVGGSYGILDMTALGRQESWELPEGRADTSRGAVPDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAE8</accession>
    <name>U5WAE8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_39545</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562496.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39545"/>
    <dbReference type="EnsemblBacteria" id="AGZ46163">
        <property type="protein sequence ID" value="AGZ46163"/>
        <property type="gene ID" value="AFR_39545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8000"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002554_0_1_11"/>
    <dbReference type="OrthoDB" id="811938at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013587">
        <property type="entry name" value="Nitrate/nitrite_sensing"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08376">
        <property type="entry name" value="NIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="552"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="690"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1033"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="729"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="758"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="845"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="950"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1061"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1115"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1204"/>
            <end position="1218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1224" mass="130460" checksum="8EBC5A366E5E0CA7" modified="2014-01-22" version="1">MSKRPKTASGVMSRLRRPAGRLRDLPIWSKLGLIMLVPTLATVIVGTSGLIDHINEASNAERARTLSVLSQAAGGLVDHLQNERAFGVMIATTKPNTPERAAADTAYKREHAKVDAAKLPYSQQKAALDDVPSQVATLLLRLDRNLEELPSTRSQTANGKLEPKAVEDDYNPLIEDLLNVRDASAQLAQDTTLSDRLRVVAAVARAKNFIALQRDVGHKVLQNNDFTPTLRRQFLETDTGYTIATSSMRQVATNDERALFDRVVNGPALRGATNLTGPLQNLGANRSKEIAFNREQWETAMIGYNDLFRSVENRLDVQIVERATEIRDDVQTRVFIETSVLLGLLLLAIVFAWLVARSMARSLRELRQGALNVAQFGLPQAVERLRDPALTGSLTPAQVADQVAEPLPVRSRDEFGQVTEAFNAVHLEAVRTAAEQAALRSSVATMFVNLARRSQILVDRLIGHLDRLERGEEDPDRLAELFQLDHLATRMRRNDENLLVLAGADSTRVQREPAALIDVLRAAQSEVEHYTRIEFGMIDRDIEVSAHAVNDMVHLVAELFDNATAFSPPNSHVIVEARRLGDGAVLSVEDRGIGISREQLRDLNERLANPPMVDVAVSRMMGLVVVARLATRHGVKVELRPADTERGTVADVGLPVSVMVMSASANRLAPAAHNPYEQQQQQRPQFAEPPLALESGSNGRGGYPAGRPFDPNPPMPAGGMLGTGGNRSVPAWSDLTGAPSNGFEPSANGFGGPSNGFGGPNGFSNGSNGSNGSNGANGFHGPRSNEPIPPLPQSPAGPQGFSGPDGLPQRRNGENQRPEDTGSYGHTIPRQLPANPETQSRSPFVPPVSAPPVPPVSAPPVPSAPPYGGGRPVSSPPASGPPASAPPYQRPESAPPAPQSAASAPPAWPPVTTDRESGHTPHVPESLAAALDMTAELPRYRADRGATGSQPQVARPTNPQNGTPQSEAERSAAAAAKAHEAAEAQAAAAQATAAAQIAAAQAAARQRAAGDSQGQFADETMELPIFRELESAWFTTARPNEPAKQQAPVEPKPKPAPSEGPDSVVTTQRFSTGEPARSAGVPQQAASPEKLNGEPAFDREPARDRDPATVGAASSAGAVNGNGSGPWQTAADDGWQAARKAAEMPVDTTTTAGLPRRTPMAQLVPGGVDRAETSVQRRTPEAVRGLLSAYHRGVQRGRTKENSTNPEETPGGQQSSQAGKEHEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAK6</accession>
    <name>U5WAK6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561318.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33615"/>
    <dbReference type="EnsemblBacteria" id="AGZ44981">
        <property type="protein sequence ID" value="AGZ44981"/>
        <property type="gene ID" value="AFR_33615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6803"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087580_2_1_11"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="20061" checksum="BB57A9641290BC4B" modified="2014-01-22" version="1">MPQIKDAAVLKAVAHPVRRRLLDVLRVDGPSMPSVLARTTSQAVANVSHHLRVLAEAGLIEEAPELARNRKEHWWRMPDRSISWQLADFDGDPADLAAADAAMAIGLQRQVELISTWLGSPASREEPWTGAAFSTDGFLRLSPAELAAMGEEIQQIVDKYAGRPEAPDREPVFVIGRGFPARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAX1</accession>
    <name>U5WAX1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45120">
        <property type="protein sequence ID" value="AGZ45120"/>
        <property type="gene ID" value="AFR_34310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6942"/>
    <dbReference type="HOGENOM" id="CLU_1944086_0_0_11"/>
    <dbReference type="OrthoDB" id="2089573at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666227" evidence="2">
        <location>
            <begin position="17"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="13488" checksum="7C413E30E9FC18E4" modified="2014-01-22" version="1">MALVAGATIGAGPAVAATSTTQGASATTQSAQQQAKTDRDRVVRIYRSERQCERAGRIGQFFGQWQRFDCERTRRGFYALEVSWRNNHGGGHGHGHGNGNGMGNGHGGGHGHGHNGGGHGHGHGGGHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAX8</accession>
    <name>U5WAX8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two component response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_34115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34115"/>
    <dbReference type="EnsemblBacteria" id="AGZ45081">
        <property type="protein sequence ID" value="AGZ45081"/>
        <property type="gene ID" value="AFR_34115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6903"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="15"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="142"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="142"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="26790" checksum="8AFEF1AADA843042" modified="2014-01-22" version="1">MTGRSINGKGQAGVRIMIADDEVAIRESLERVLQVEGYDTSTVANGLAVLDGVGGAGGDTLDLLILDVMMPRLGGLETCRRLRAAGQDLPVLMLTARDQVSDRVAGLDAGADDYLPKPFATEELLARVRALLRRRAPGDGESQILSFADVRLDPDRFEAWRGARPLRLTRTEFSLLQVLVRNATRVLTRDALFEAIWGFDMSATANNLQVYVSYLRRKMEAEGEPRLIYTLRGLGYTLRETPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBA6</accession>
    <name>U5WBA6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561596.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35020"/>
    <dbReference type="EnsemblBacteria" id="AGZ45260">
        <property type="protein sequence ID" value="AGZ45260"/>
        <property type="gene ID" value="AFR_35020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7086"/>
    <dbReference type="eggNOG" id="COG4894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412529_0_0_11"/>
    <dbReference type="OrthoDB" id="1343711at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="21930" checksum="377FC8660283A324" modified="2014-01-22" version="1">MTVDTLQTQQQLIVRQRLRLMVNQYEVHAVGPDGSEAGVLAFAQQKRMAFKEQVTLYTDDTKSTPVLGFKARQRLDLAATYDITDAAGSPIGLFRKNFKESLLRSTWHLEQPGYGEMVGRETNMTVAILRRFVDSLSWLPYHFEFLLNDRPAFSVVKKWGLRDKYVVTIHDPQLDRRLVAAMAVALDALQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBG3</accession>
    <name>U5WBG3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_35150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561622.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35150"/>
    <dbReference type="EnsemblBacteria" id="AGZ45286">
        <property type="protein sequence ID" value="AGZ45286"/>
        <property type="gene ID" value="AFR_35150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7112"/>
    <dbReference type="eggNOG" id="COG0655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051402_0_2_11"/>
    <dbReference type="OrthoDB" id="796211at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="6"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21446" checksum="140DB27B6F3D5D52" modified="2014-01-22" version="1">MAAVKLAIIYYSSTGTIHSMAERLSQAGEKAGAEVRVRQVGELAPPEAIASNAAWSQHFDKTKNEPKATADDVVWADAVLFGTPTRYGNVASQLKQFMDTLGPQWQQGLLADKAYAGFTATMTAHGGQESTLLALYNTIHHWGGVVAAPGYTDPLKFADGNPYGVSHVTGGNNDAPLTEVQNAALDHLAQRIIKIGGRLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBG7</accession>
    <name>U5WBG7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 9">Uroporphyrinogen decarboxylase</fullName>
            <shortName evidence="8">UPD</shortName>
            <shortName evidence="8">URO-D</shortName>
            <ecNumber evidence="4 8 9">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemE</name>
        <name evidence="13" type="ORF">AFR_35175</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>4 H(+) + uroporphyrinogen III = 4 CO2 + coproporphyrinogen III</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8 9">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 10">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="4 8 9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35175"/>
    <dbReference type="EnsemblBacteria" id="AGZ45291">
        <property type="protein sequence ID" value="AGZ45291"/>
        <property type="gene ID" value="AFR_35175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7117"/>
    <dbReference type="eggNOG" id="COG0407">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040933_0_1_11"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 8 9" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen_deCOase" evidence="11">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Uroporphyrinogen_deCOase" evidence="12">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="20"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ45291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="334" mass="35821" checksum="D8F641B061C34709" modified="2014-01-22" version="1">MFVRACRGRSVPHTPVWFMRQAGRSLPEYRKIREGVGMLESCRRPDLVTEITLQPVRRHGVDAAILFSDIVVPLAAAGVDLDIVPGTGPVVAEPIRTAADVAALGTLEPGDVSYVDEAVRLLVPELGATPLIGFAGAPFTLASYLIEGGPSRTYLKTKAMMYGDPDLWNELCGKLADITLAFLRVQVGAGVSAVQLFDSWAGALSEADYRRFVLPHSARVLSGLQDAGVPRIHFGVGTGVLLQAMGEAGADVVGVDWRTPLDRATTVIGPDRSVQGNLDPAILFAPWEVVEREARRVVAEGRAAPGHVFNLGHGVMPETDPEVLTRLVELIHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBH2</accession>
    <name>U5WBH2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561671.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35395"/>
    <dbReference type="EnsemblBacteria" id="AGZ45335">
        <property type="protein sequence ID" value="AGZ45335"/>
        <property type="gene ID" value="AFR_35395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7162"/>
    <dbReference type="HOGENOM" id="CLU_184971_0_0_11"/>
    <dbReference type="OrthoDB" id="2089437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="9420" checksum="B91FBC9F4339D846" modified="2014-01-22" version="1">MSDNSDYAPETLDEPSTSRPEKGNDDPGLSSTIAAGTDTDQLINGGPPPRDGGAVTTTGGTAGPNSFRPKARHGEGVANTTTGDATTGSMEPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBZ0</accession>
    <name>U5WBZ0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42295"/>
    <dbReference type="EnsemblBacteria" id="AGZ46713">
        <property type="protein sequence ID" value="AGZ46713"/>
        <property type="gene ID" value="AFR_42295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8560"/>
    <dbReference type="eggNOG" id="ENOG502Z890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063460_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1996" evidence="1">
        <location>
            <begin position="30"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="31160" checksum="220280D757539BCB" modified="2014-01-22" version="1">MKPSIEVPAHRMLHEFQANCAVSRTNLPDDPIVFPKLPGASHSHTFMGNTTTNAATTTESLSAGDTKCLAEGDLSAYWMPTLYKGDGAINPVGAQTIYYKTGVNDYTSVRPFPTGLRFVVGNAKNTDPAAFLKATVEGFECGESYKNADFPASCPSTASTQLNIRYQAPSCWDGIHLDIPDHQSHMAYPTIKAHPIGAACPDDHPVAVPMLEFKMAWPVNGDMSKVRFASGAGYTFHYDFMNAWDPKTLEALVKACINTGRQCNARGYDEREPASTAVLNENYQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC64</accession>
    <name>U5WC64_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4173 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_42450</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42450"/>
    <dbReference type="EnsemblBacteria" id="AGZ46744">
        <property type="protein sequence ID" value="AGZ46744"/>
        <property type="gene ID" value="AFR_42450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8591"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025121_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025231">
        <property type="entry name" value="DUF4173"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13777">
        <property type="entry name" value="DUF4173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4173" evidence="3">
        <location>
            <begin position="288"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="533" mass="56682" checksum="5689EF9605A17EBF" modified="2014-01-22" version="1">MPQGATALATRPQPAQTGPGMPVAPLHGPWANPSIFGHRWPGPALPPAPAAISALLIAGVMAALSIPLDRAGIGWLITAIAGVAALTAARMAPHRISPTAPKPLVTRTRNTLTRARFGWSAATVALLGVGTFRSAGWLFVLCLLTAILTGALAVAGGRSLRSMSVATIMTPLAGFRALNWIRRGLATAGRAGGSGLRITATVAVSLVLLLVFGSLFASADAAFADLIEAAVPDISAITIVRWIFVFLLTTFILGGAAFLRAAPPNLDGLDSPGKRRVARLEWAVPLTLLVLLFAMFVAVQLTVLFGDSKHVLDTDGLTYAEYARSGFWQLLVVTGLTLVVLAGAARWAPRGTSTDRLLIRGILGLLAALTLVIVASAIHRMDLYADTYGLTRLRVLVAVCELWLGLTFVLILIAGIRLKAAWLPRAVVALGVLSLLSLAVANPDHLIADRNITRFEQTGRIDRSYLSDLSPDAVPALTRLKGDDRTCTLIPIARDLADNTDDWRAWNLGRHQAREALKANPPYPTRPCLDLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCE0</accession>
    <name>U5WCE0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36625"/>
    <dbReference type="EnsemblBacteria" id="AGZ45581">
        <property type="protein sequence ID" value="AGZ45581"/>
        <property type="gene ID" value="AFR_36625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7409"/>
    <dbReference type="eggNOG" id="ENOG502ZZW3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1238045_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24504" checksum="D806F3F905AF98D9" modified="2014-01-22" version="1">MSRYRLVTSELFRTYRPVALWFWSIMIVGVAIGSTVVNLVTEPKFSLWLVVVGASAKWWLGVVGVLLVSLHLRQFVANGITRRDFMTGMAVFGLAGSLLTALIVPIGHALEQLLLGIAGPLPPDYPALSAGAAALEFGHVLPASLAFFVAGATATAGFYRYGAWGGLALVVPAVLPIGFAEVLLGVDQHGGFDSRFVPYAAAAALSLVVTGLVALLHEREMRHVAIRRGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCU6</accession>
    <name>U5WCU6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 17">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="6 17">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">AFR_37485</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-homoserine + NAD(+) = H(+) + L-aspartate 4-semialdehyde + NADH</text>
            <dbReference type="Rhea" id="RHEA:15757"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 17">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="6 17"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562086.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37485"/>
    <dbReference type="EnsemblBacteria" id="AGZ45751">
        <property type="protein sequence ID" value="AGZ45751"/>
        <property type="gene ID" value="AFR_37485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7582"/>
    <dbReference type="eggNOG" id="COG0460">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_1_0_11"/>
    <dbReference type="OrthoDB" id="1464088at2"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016204">
        <property type="entry name" value="HDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000098">
        <property type="entry name" value="Homoser_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14 17" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 17" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="13 17" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11 16 17" id="KW-0521">NADP</keyword>
    <keyword evidence="12 17 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="19">
        <location>
            <begin position="357"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="13"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AGZ45751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="435" mass="44983" checksum="00FB81549C6E4ABE" modified="2014-01-22" version="1">MSSEKPAVRLALLGCGTVGSEVVRLLHEQAADLTARIGAPLELVGIAVRRPGRQRGTIDPALFTTDALGLVKRDDVDVVIEVVGGIEPSRTWLTEALRAGKSVVTANKALLAEDGATLHDAAAEGGADLYYEAAVAGAIPLLRPLRESLHGDRVTRVTGIVNGTTNFILSSMDATGAGFGEALDEATELGYAEADPTADVEGFDAAAKAAILASLAFHTRVTAADVFREGMTGVTAADMASAKEMGCTIKLLCIAQRGPDSTGNESVSVRVHPAMIPRSHPLAGVGDAFNAVFVEAEAAGQLMFYGRGAGGTPTASAVLGDIVAAARNRISGTRAPSESSYAHLPIRPIGESVTRYHISLDVADRQGVLATVAGVFAKHEVSIATVRQSGREEDATLVIVTHGAPDANLAATVEDLRSLDIVRSIASVLRVEGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WED2</accession>
    <name>U5WED2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5667 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40210"/>
    <dbReference type="EnsemblBacteria" id="AGZ46296">
        <property type="protein sequence ID" value="AGZ46296"/>
        <property type="gene ID" value="AFR_40210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8138"/>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061725_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043725">
        <property type="entry name" value="DUF5667"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18915">
        <property type="entry name" value="DUF5667"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5667" evidence="2">
        <location>
            <begin position="132"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="388" mass="40644" checksum="1F91A71D006B995E" modified="2014-01-22" version="1">MLTVNLNIFDRRSAERFAELLDETNGGRRHHARGQADEQLAELVAIGHSLSAARPGAQVDPEFRMGLRAMLVATAERDGIGATATDREQQAEPAVPARKLFGRRIRARGAIVIGVAAGAMAVSGISAASENAAPGDALYGVKRSTERAQLAMAGSDVTRGQLSLTFARNRLAEAAAMQGDNPKFGDVLDDMDNDTRQGVKLLTSSAVTRKDKAPLNSVENFVESQRRVITPKLDKLNTNNRERALGSMSLLDSVQERSKDLRSGLQCDTVTQSGSDAIGPKLRQCATGAANSTSPGQQQGQGQKEATTGSGTKGDDEGKKAKPKKTGEPTADPTTDGEPGTPGATDRSADPTSTDSPEPLISDIPLDDGGTADEDKGLLGGLLGIFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WED3</accession>
    <name>U5WED3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_40730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydrogen cyanide + thiosulfate = 2 H(+) + sulfite + thiocyanate</text>
            <dbReference type="Rhea" id="RHEA:16881"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:18022"/>
            <dbReference type="ChEBI" id="CHEBI:18407"/>
            <dbReference type="ChEBI" id="CHEBI:33542"/>
            <dbReference type="EC" id="2.8.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562732.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40730"/>
    <dbReference type="EnsemblBacteria" id="AGZ46400">
        <property type="protein sequence ID" value="AGZ46400"/>
        <property type="gene ID" value="AFR_40730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8245"/>
    <dbReference type="eggNOG" id="COG2897">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031618_1_3_11"/>
    <dbReference type="OrthoDB" id="1385159at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="18"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="160"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="31343" checksum="59F12F34701F0CD2" modified="2014-01-22" version="1">MSRDTALVSADWAEKNLETPGVVFVEVDEDTSAYDGGHIPGAIKIDWKSDLQDPVRRDFVNQEQFSQLLSERGISNDDTVILYGGNNNWFAAYAYWYFKLYGHRDVKLIDGGRKKWELDARPYSKDVPARAKTQYQASSPDLSIRAFRDEVVQAIGVKNLVDVRSPDEFAGRLLAPAHLPQEQSQRGGHIPTALSVPWSKAANEDGTFKSDDELAKIYGDAGLDGSKDTIAYCRIGERSSHTWFVLKELLGHDNVKNYDGSWTEYGSLIGVPIALGDEPGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEI3</accession>
    <name>U5WEI3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fliO</name>
        <name evidence="6" type="ORF">AFR_40510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FliO/MopB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562688.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40510"/>
    <dbReference type="EnsemblBacteria" id="AGZ46356">
        <property type="protein sequence ID" value="AGZ46356"/>
        <property type="gene ID" value="AFR_40510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8199"/>
    <dbReference type="eggNOG" id="COG3190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131987_0_0_11"/>
    <dbReference type="OrthoDB" id="2084487at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022781">
        <property type="entry name" value="Flagellar_biosynth_FliO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04347">
        <property type="entry name" value="FliO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03500">
        <property type="entry name" value="FliO_TIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="1 5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="15239" checksum="C060302C5F5BB205" modified="2014-01-22" version="1">MFELVLRIGFSLLVVFGLMWGLAKVARRGGVGRRGSGSLSVLNRQSLSRGSSVAVVQVADRALILGVTDTQVSLLGETDLDAFGAGAHPAPAKRDTLAPGADDVLPPAIPTVLPHHSGKLDGSILSPRTWSSTLDFLRDRTTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF95</accession>
    <name>U5WF95_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PadR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563041.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42280"/>
    <dbReference type="EnsemblBacteria" id="AGZ46710">
        <property type="protein sequence ID" value="AGZ46710"/>
        <property type="gene ID" value="AFR_42280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8559"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_10_0_11"/>
    <dbReference type="OrthoDB" id="1706427at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="25"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="115" mass="12702" checksum="19039883F0DDF835" modified="2014-01-22" version="1">MTEQPGRITAPLLDVLATFIAAGGNELHGWAIIKESKRGGPTVYKILERLTGMGWITARWEDLPTEPDPSEPNKPRRRYYKLTGEGATRAKALLAERRPATTRATLRPALEGGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFA1</accession>
    <name>U5WFA1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cellulose-binding family II protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_42305</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42305"/>
    <dbReference type="EnsemblBacteria" id="AGZ46715">
        <property type="protein sequence ID" value="AGZ46715"/>
        <property type="gene ID" value="AFR_42305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8562"/>
    <dbReference type="eggNOG" id="COG3858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019399_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666300" evidence="1">
        <location>
            <begin position="16"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="12"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="3">
        <location>
            <begin position="163"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="450" mass="46513" checksum="818EA9FCE2EDC0D4" modified="2014-01-22" version="1">MVGALGVAGVTNAMAATTGTITGLGGKCLDVTDGSTANGNLPQLWTCSGGPNQSWTIADNGSIQGRGKCLDVANNSTADGGTVHLWDCYDTVASQKWTVSNGALVNTASGKCLDVKDNSTANGAKLQIWSCTGAANQKWTTAAGGGGGGTTPPPTTGGPGAKAVAPYYYTGWGNPPNLTTVKNATGVTWFTMAFMLNSGNCDPKWDGSRPLTGGADQTAINTIRANGGDVVISFGGAAGPWLEHYCTSATALAGAYQKVINAYGLKAIDIDIEGTPYNTPADQQKTVDALKIVKANNPGITTYITLGSGTSGPDNSLINRAAGAGLQVDGWGIMTFDWGNTTGNQGSLTIQAADALKNKLKTAYGYSDADAYRHVGISSMNGITDEKAVVSLDNMRAITAYAQQHQIARLTFWSLNRDRQCSGAYPNDDTCSGVSQSAYEFTKIISAYKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFF9</accession>
    <name>U5WFF9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyl-glucosamine transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562982.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41985"/>
    <dbReference type="EnsemblBacteria" id="AGZ46651">
        <property type="protein sequence ID" value="AGZ46651"/>
        <property type="gene ID" value="AFR_41985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8495"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023978_3_1_11"/>
    <dbReference type="OrthoDB" id="724641at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="48765" checksum="B93BC6F0EC18B52A" modified="2014-01-22" version="1">MTAAHSWYLVVVGYAIMLAWPVSTLALVSFAVRYVASHRSVVVGKALEHGDGTPQHFWIIVPALNEEVVVGNTVNAALRLSGPGGTLARVLVVDDGSDDRTPEVLAAIDHPRLQVLRRELPNARKGKGEALNAAYRYIAERTAEAGISPDKVVIGIIDGDGQGSHNILLEVSRMMRDTKIGAVQVQVRIRNRNKLLGAVQDLEFGAIVNACQTLRDTIDTVGLGGNGQFTRLSALLALGDAPWSACLVEDLELGLRMHLDGVGIRYTSRASVTQQAVVDLRRLTRQRTRWAQGNIQCVRYLRQLMASKRISTTSLLEVMHYLISPWINAVVTVVLLVAVTVATAGLLIGHPVPYLHTWGALGVSGGTWFAVTFFPGFVWALVHRYRLHDESLGRLLTAAAAYPIFLLLGLVATYRAIGRQASGRQAWAKTERLAEEPLPQHLQVALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNK2</accession>
    <name>U5VNK2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00400"/>
    <dbReference type="EnsemblBacteria" id="AGZ38369">
        <property type="protein sequence ID" value="AGZ38369"/>
        <property type="gene ID" value="AFR_00400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.82"/>
    <dbReference type="eggNOG" id="COG2350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110355_6_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="93" mass="10015" checksum="0D01D58111D202A1" modified="2014-01-22" version="1">MYLMISTYLAPLDQVDQAREAHLAFVGELESAGVLVSAGRQNPPAGGVILLNVDTEAEARELMAPDPYLQQGLAEYAATGWTPTRGVLADWKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNZ8</accession>
    <name>U5VNZ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_01125</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357457.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01125"/>
    <dbReference type="EnsemblBacteria" id="AGZ38514">
        <property type="protein sequence ID" value="AGZ38514"/>
        <property type="gene ID" value="AFR_01125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.224"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_11"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="33"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="307"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ38514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="402" mass="41221" checksum="320B310F35AD5266" modified="2014-01-22" version="1">MSALTVRRSSLHPGDLLRVGSIGLRTRRMRATLSALGIAIGIAAMVSVLGISESSKADLLSSLDRLGTNMLTVEAGTGIGGGGTTAVLPTSTETMLRRITPVEQVASVTPVDATVRRTDLVPETHTGGIAVKAADPALLDTLAARPADGVWLDPARAALPVVVLGSVAAERLGITEVADGTRIYVGDQWFAVIGILESVELAQDIDRSVLIGRPIAASKFNTDEAPGTVYIRSDPDAVDDVRAVVAATANPANPDQVTVSRPSDAIEARAAASNAFTSLFLALGAVALLVGGVGIANVMVISVLERRSEIGLRRALGATRRHVSIQFLAEALLLSALGGLGGAILGALVTAGYDLLRGWDVLIPYVGMAGGVVAAVLIGAAAGLYPAVRASRLSPTEALRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP06</accession>
    <name>U5VP06_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357621.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01945"/>
    <dbReference type="EnsemblBacteria" id="AGZ38678">
        <property type="protein sequence ID" value="AGZ38678"/>
        <property type="gene ID" value="AFR_01945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.394"/>
    <dbReference type="eggNOG" id="ENOG5031TFI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1358049_0_0_11"/>
    <dbReference type="OrthoDB" id="1605238at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21090" checksum="96321E0D26821F6E" modified="2014-01-22" version="1">MDRLDRILDTAAPLLRRIDAILAAGGAPPQHRVWAELRRVRLLPGDAVRSVAVLRPEELLDAAPELRADARAYVVLAASLPGPGEWAGAAADNYDRARSRAADHLSGTEQSLGARLDRTADLADNLIAWMQDTRDAVADALAQALSSTEAIALAGDKPDLASARELEAAADVAALLLAAVADGCVTATDLLHDTAELAEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP38</accession>
    <name>U5VP38_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative cytochrome c biogenesis membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_01340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357500.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01340"/>
    <dbReference type="EnsemblBacteria" id="AGZ38557">
        <property type="protein sequence ID" value="AGZ38557"/>
        <property type="gene ID" value="AFR_01340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.268"/>
    <dbReference type="eggNOG" id="COG1333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023092_0_0_11"/>
    <dbReference type="OrthoDB" id="1518243at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023494">
        <property type="entry name" value="Cyt_c_bgen_Ccs1/CcsB/ResB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007816">
        <property type="entry name" value="ResB-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31566">
        <property type="entry name" value="PTHR31566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05140">
        <property type="entry name" value="ResB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="ResB" evidence="7">
        <location>
            <begin position="36"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="539" mass="59484" checksum="CC49F8F2037FD64A" modified="2014-01-22" version="1">MTVVEERPAPADAPPPRRPNRAWALLRNSWRQLTSMRTALVLLFLLAVAAIPGSVLPQRSVNAENVAGYFREHPELAPFLDRLGGFDVYASPWFAAIYLLLFTSLIGCVVPRLADHFRALRTVPPEAPRRLERLPQHAAAEARPDDPAAAAATIAATLRRRRFRTVVRERSDGTWTVSAEKGYLKETGNLFFHFALLAVLVGVGFGHWYGWHGNRLLVQGNDRGFCNSVTQYDESSLGPRIDASNLPFFCLRLTDFSATFQDTGQPKSFGAKVDVQDKQDGPWRAEEFTVNHPLRLPQASVHLLGHGYAPILRYTDRFGEQQTRIVPFLPRDAMQTSEGVVQFPDVNIDPKTNKRDEDLQVAFEGVYLPTVDPKGTPNSVFPAERDPALYLSAYRGNLGVDAGLPGSVYALDRSQLANGQLKQVSGVRPQRLAVGQTWTLDDGTKLEFLGTRQFATLSVRYDPTQPLVLGGAVVGLLGLMLSLGGRRRRVWFRVSPPETSGAGDGGSLMEAGGLPRTDYPGFEEEFAQVVAAVRDERTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ06</accession>
    <name>U5VQ06_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03015"/>
    <dbReference type="EnsemblBacteria" id="AGZ38892">
        <property type="protein sequence ID" value="AGZ38892"/>
        <property type="gene ID" value="AFR_03015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.606"/>
    <dbReference type="eggNOG" id="ENOG502ZIHB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122411_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046172">
        <property type="entry name" value="DUF6174"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19671">
        <property type="entry name" value="DUF6174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665210" evidence="1">
        <location>
            <begin position="25"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17459" checksum="CB0403A78BF7F305" modified="2014-01-22" version="1">MEGTVRLRTVLPGLLLITLTGACANDEPNVMNEPPVTWTVPDKYAFTLASECGERALIGRFQVSVVGDKVVAAKGLDDSARNALMLRMADLVPTLQQLEAEAETARREGAEVVEVERDLGDAHPAKITIDYSRNAIDDEACYTIEDYTIGLTEAPSPLPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRL7</accession>
    <name>U5VRL7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357245.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00060"/>
    <dbReference type="EnsemblBacteria" id="AGZ38301">
        <property type="protein sequence ID" value="AGZ38301"/>
        <property type="gene ID" value="AFR_00060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.12"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_40_2_11"/>
    <dbReference type="OrthoDB" id="1501005at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="162" mass="17886" checksum="5709C03843483DF8" modified="2014-01-22" version="1">MTRLERLDERHADELLRFERENRAFFARSIPDRGDDYFVSFAERHAALLTEQASGVIQFHVMVDDNGAVLGRFNLIDLVDGQAELGFRVAEHATGRGLATAGVQEILVLARDDYGLDRLEASATLGNAGSLGVLRRTGFVAVGEVVLNGLPGLRHVRDLEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRS7</accession>
    <name>U5VRS7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Daunorubicin resistance ABC transporter ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_06165</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359053.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06165"/>
    <dbReference type="EnsemblBacteria" id="AGZ39522">
        <property type="protein sequence ID" value="AGZ39522"/>
        <property type="gene ID" value="AFR_06165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1252"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DUF4162"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DUF4162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32080" checksum="AD31E78C2F9D5D83" modified="2014-01-22" version="1">MGVRTEGLSKAYGGVTVLRGIDLQVAPGSVFALLGPNGAGKTTLVRILTTLTRPDAGTATVAGHDVLRDPRGVARAITLTGQFAAVDELLTGVENLVMLGRLRRLGRAGARRRAHELLEQFDLTDAGGRLAKTYSGGMRRRLDLAMSLVRRPEVLFLDEPTTGLDPRSRQTTWAAVRALAAEGVTVFLTTQYLEEADQLADRVAFLDRGAIIADGTPRRLKAQLAGERVELHLPAGQLGRAATVLGSGATSDATRGTVTVGTSGSAAEVRELLNLLHTHDIPVDTVAVHRPTLDDVFLTVTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRT0</accession>
    <name>U5VRT0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">1-aminocyclopropane-1-carboxylate deaminase</fullName>
            <ecNumber evidence="9">3.5.99.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_06190</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-aminocyclopropane-1-carboxylate + H2O = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16933"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58360"/>
            <dbReference type="EC" id="3.5.99.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ACC deaminase/D-cysteine desulfhydrase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.7" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359058.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06190"/>
    <dbReference type="EnsemblBacteria" id="AGZ39527">
        <property type="protein sequence ID" value="AGZ39527"/>
        <property type="gene ID" value="AFR_06190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1257"/>
    <dbReference type="eggNOG" id="COG2515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048897_2_1_11"/>
    <dbReference type="OrthoDB" id="1714795at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008660">
        <property type="term" value="F:1-aminocyclopropane-1-carboxylate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009310">
        <property type="term" value="P:amine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027278">
        <property type="entry name" value="ACCD_DCysDesulf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005965">
        <property type="entry name" value="ACP_carboxylate_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780">
        <property type="entry name" value="PTHR43780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006278">
        <property type="entry name" value="ACCD_DCysDesulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01274">
        <property type="entry name" value="ACC_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="8">
        <location>
            <begin position="11"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006278-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006278-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="35756" checksum="4F067C45696FD61D" modified="2014-01-22" version="1">MALDDFDRYSLLFGPSPVHRLDRLTRHLGGAAVWAKREDCNSGIAYGGNKTRKLEYLVADAIAQGCDTLVSIGGVQSNHTRQVAAVAAYAGRKCVLIQESWVDWPDSVYDKVGNILLSRLAGADVRLVKAGFGIGFKESWDAAIAEVEAGGGKPYAIPAGASDHRLGGLGFANWAREVAEQEAQLGVFFDTVVVCSVTGSTQAGMIAGFAALGDHRPRRVLGIDGSAKPVETHDQVSRIARATAALIGVDRDLRDDEILLDDRYHGGIYGIPDEATLDAMRVAARTEGMITDPVYEGKSMAGLIDLVSRGEISKDSTVLYAHLGGQPALNAYSAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS29</accession>
    <name>U5VS29_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359180.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06800"/>
    <dbReference type="EnsemblBacteria" id="AGZ39649">
        <property type="protein sequence ID" value="AGZ39649"/>
        <property type="gene ID" value="AFR_06800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1389"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033160_3_0_11"/>
    <dbReference type="OrthoDB" id="1370117at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="391" mass="42518" checksum="8BE7A29F15275E3A" modified="2014-01-22" version="1">MTYYVYVEPDAERRNTIGGLYSDFGFDIVADLADLEAHLPRNPETLLVMIGATVDLDAALTFTAYQRVQRPLIGVLLLRRTLEPDVLARAMRSGVREVVEELDVEGIRNSAARSIDLSKALSTTMRAASDQGQRAKIVTVFAGKGGCGKSVVATNLAAALSADGSRRVLLVDLALQFGDVAIMLQLPPNRSVSDAISMAGKLDEAGLRSVLTPYRPGLDALLAPASPAEGEHVSRELVTEILEVARGMYDFIVLDTPPAVTDQVLSALDLSDWFVPIVTPDLPTLKSVRLTIEMFDLLEYPRDRRLVVFNRSNTEVGLTTDDVEEAVGAHLSVHMPSSRDVPLSVNRGVPMVLSDPTHPVSLAVRKLADRCAGVTSSQESRRKIFAFGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSV9</accession>
    <name>U5VSV9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359433.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08065"/>
    <dbReference type="EnsemblBacteria" id="AGZ39902">
        <property type="protein sequence ID" value="AGZ39902"/>
        <property type="gene ID" value="AFR_08065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1646"/>
    <dbReference type="eggNOG" id="ENOG5032U4U">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104647_0_0_11"/>
    <dbReference type="OrthoDB" id="1956015at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="203" mass="22042" checksum="7EF37CC3F4354A0A" modified="2014-01-22" version="1">MKMYADRLPTAVRQLLTDLLVVFWVYAWIRAGIWVNDMVEKLGVPGQKLQEAGSGIAGNMSDIGGKVGRVPLVGDELTAPFNSAADAANQLAEAGRQQQEIVDNLALTMALLIIAVPLGLVLFLWLPLRLRWMRRAAVARRVSSEPAGQDLLALRALATRPLNELSKLGPDIAQSWRNGDASAVQALAALELKDLGLRTAKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT48</accession>
    <name>U5VT48_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cortactin-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_08490</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359518.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08490"/>
    <dbReference type="EnsemblBacteria" id="AGZ39987">
        <property type="protein sequence ID" value="AGZ39987"/>
        <property type="gene ID" value="AFR_08490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1728"/>
    <dbReference type="HOGENOM" id="CLU_938853_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="3">
        <location>
            <begin position="261"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="30441" checksum="E2789A4DA2B51A59" modified="2014-01-22" version="1">MTSTRTRPATVTVATALMAFTATGYLITAIALFGQVGHTRTWAGDKFRDFGGDTGTALVFAESTTVIVAVLTIVAALTLLASAASVRAGSQPGRIFAFTMMAILLLCGISAATRGGTPDFGNNVTMSTSRSNGTVTRTTVSALPESYGSAYRIGSGVFAGLAMLAMIAAIILLTRPSANRWFRPEPLPAGPPAGYRPYAAQPSPPPGHFGAPPPPAQFAGPPPAGTFAGPPPPGAYGGPPPPETFVPVAVPPQPPQWHAGPAPAADSEIAVLTRRHQRGELTDAEYAAGLNRLRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTH3</accession>
    <name>U5VTH3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase-related FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357904.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03360"/>
    <dbReference type="EnsemblBacteria" id="AGZ38961">
        <property type="protein sequence ID" value="AGZ38961"/>
        <property type="gene ID" value="AFR_03360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.676"/>
    <dbReference type="eggNOG" id="COG3467">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115408_0_0_11"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="26"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="18271" checksum="C18B16865931FB6D" modified="2014-01-22" version="1">MTAPIVEFNEGFSEPGAGPVPWADVDDLLTKSEMFWLSTVRGDGRPHVTPLPTVWLDNILHFCAGDQEQKTKNLLGRPACVLTTGTNDLHSGVDVVVEGVAERITDHARLVELAALWKSKLDWDYVVGEDAFGDGAGRTGLVFGVRPRKVLSFGKNPYTQTRYRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTX3</accession>
    <name>U5VTX3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39160">
        <property type="protein sequence ID" value="AGZ39160"/>
        <property type="gene ID" value="AFR_04355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.879"/>
    <dbReference type="HOGENOM" id="CLU_764811_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665321" evidence="1">
        <location>
            <begin position="26"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="42095" checksum="93B5377F5A769216" modified="2014-01-22" version="1">MLHGTAAAILTAVLAVTLAPQPAQAAIAVIGTVYKEPQPYEARFPDIIKLDDGNLMAVWHRAELHVGTVEKPANGTIQLSIWNGTVWSAPVLALAHPRFLGDVDMRDPKLAKMNDGQVIMTYFVGAKLYYSYWREGWTRFTDPLQLTMPGAPASMASHGSVLPLQQGGNPTDQVLVPFYSSGTEGGAFYLRATYHPNQTERLEPTFVKRIIKNDNPVGRRYTEPSFVQIGEKIVAGIRSENKVDGNDELQARPLAIGRWDAHDDTTNPTFELFQLGAGNPVLASSHHLLKTVKNGQEKIFFTYGNRFTPTRRPTYGIMIDNPLATWTGTSRLLYDSGAYDQANPSSVEAAEGWYWTLAFNANPGSTDNGRLLMVRSLHTDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU62</accession>
    <name>U5VU62_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_04805</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MmpS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04805"/>
    <dbReference type="EnsemblBacteria" id="AGZ39250">
        <property type="protein sequence ID" value="AGZ39250"/>
        <property type="gene ID" value="AFR_04805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.970"/>
    <dbReference type="eggNOG" id="ENOG502ZWSU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097057_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008693">
        <property type="entry name" value="MmpS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038468">
        <property type="entry name" value="MmpS_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05423">
        <property type="entry name" value="Mycobact_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="29098" checksum="61FF9094ED166961" modified="2014-01-22" version="1">MSGQPWSSPSGDPLAAEPYQPPPPPAPTFQPGYASLPTPSEQASGPPDGYPPTSMFPADPGQYPPPGYQPPLGQAPPGQMPPPGYGPPPGYGPPPGYGAQPPPPPKRSNAPLIAVLIAVTLLLCAGGVTSAVILVNNATNKAKETIDSLPRPTLPTELPTDLPTDLPTELPTDLPTGLPNLPDVDPNQQIEVEYEVTGDGPVEIVYMEELGGEPQRVRNASLPWTKKLTMKGSSLVSVVAVRGSTEEGELNCSAKVDGEEVAQKSTSGTFITASCTKVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU75</accession>
    <name>U5VU75_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_10675</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360289.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10675"/>
    <dbReference type="EnsemblBacteria" id="AGZ40424">
        <property type="protein sequence ID" value="AGZ40424"/>
        <property type="gene ID" value="AFR_10675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2176"/>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_2_11"/>
    <dbReference type="OrthoDB" id="1681886at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="4">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="110" mass="11626" checksum="561724032B2B9AEC" modified="2014-01-22" version="1">MSFERVGPAADLAKGSVTTAEIDGVDVAIVHADDGQFYAVRDECSHASIALSEGEVDGCTLECWLHGSRFDLRTGEPSGLPAIEPVATFPVEIRDGDIYVSMTPSNGVMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUA3</accession>
    <name>U5VUA3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39300">
        <property type="protein sequence ID" value="AGZ39300"/>
        <property type="gene ID" value="AFR_05055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1021"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_10_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="123" mass="13390" checksum="6FC4CB9967223B89" modified="2014-01-22" version="1">MVVENYDSAIDFFVGALGFDLIEDSPAKTTDGRPKRWVVVRPPGAVTGLLLAEADSDTQRAAIGNQLGGRVGFFLNVDDFDAQFKRMTDAGVHFNEEPRSEPYGKVVVFTDIAGNKWDLLGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUJ0</accession>
    <name>U5VUJ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative isobutyryl-CoA mutase chain B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_05085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358815.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05085"/>
    <dbReference type="EnsemblBacteria" id="AGZ39306">
        <property type="protein sequence ID" value="AGZ39306"/>
        <property type="gene ID" value="AFR_05085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1028"/>
    <dbReference type="eggNOG" id="COG2185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128233_0_0_11"/>
    <dbReference type="OrthoDB" id="1335734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006159">
        <property type="entry name" value="Acid_CoA_mut_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="3">
        <location>
            <begin position="4"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="14190" checksum="2078BE3495073D2D" modified="2014-01-22" version="1">MQARIRVVVAKPGLDGHDRGAKVVARALRDAGMEVVYTGLHQTPEQIVETAIQEDADAIGLSVLSGAHMTLFRRVVELLAERDAADIVVFGGGIIPADDITELEKLGVAHIFTPGATTASIIEWVRANVATPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVG1</accession>
    <name>U5VVG1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_07030</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07030"/>
    <dbReference type="EnsemblBacteria" id="AGZ39695">
        <property type="protein sequence ID" value="AGZ39695"/>
        <property type="gene ID" value="AFR_07030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1434"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_12_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="PTHR23505"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="456" mass="47967" checksum="8EF7274597B32D65" modified="2014-01-22" version="1">MIVFVVLASLDNVAIGLVPPLYGSIGRDLGVGEGRIALATTVMFLISAVAAIGWAYAGDRTDRKPVLIAGTLIWVLGTAWSGLAGSYPSFLLSQVLAAIGLGGVASVSFAVVSDLISPRRRGLVMSFWGLSQGVGTLAGTLVGGLLGSSDWRRPFLIVAVAGVVATVAYVLTYNVPRGDSQPELEGVEYDERIHRDDLPRILARRTNIWLILQGFTAQIAFGSLVWLPVLFRARAEDQGYSAATAVIVGSVFATLFQLGGALSILGGLVGDRLQRRTPRGRALVAAVGVLAAVPFYVVLFFVPFRIDVPDGGSTGAVIRAILRNVWTEPTVGLSLATAIFALALTSANSPNWFALIADVNPPEHRGTVYSLGNLVNGFGRAGGNAFIAVAFRGLSGAFPPPLNYAVGLAAFQLFFIPTGVMYWLAGRTVARDMADTHAALATYSAKADEVRPAPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVH1</accession>
    <name>U5VVH1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_12915</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360928.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12915"/>
    <dbReference type="EnsemblBacteria" id="AGZ40869">
        <property type="protein sequence ID" value="AGZ40869"/>
        <property type="gene ID" value="AFR_12915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2623"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_2_4_11"/>
    <dbReference type="OrthoDB" id="1558246at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="24"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="514" mass="53196" checksum="43BE815F36FE391B" modified="2014-01-22" version="1">MTVLNDAPAPAGPAPMTRAQIRQALSGLYLGIFVAILSSTIVTNALPAIVADLHAGQSVYTWIIIATLLAMTVSAPVWGKLADLVSKKLLIQLGLTIFTAGSVLSGLASDPALLIAARAVQGIGAGGLLALIQVIIATMIPPRELGRYSGYFGAVLAVGTSAGPLLGGFIVDTGWLGWRWCFFVGVPFAVAALVVLQKTLHLPVAKRPARIDWAGTTLLTAAASLLLLWVTFAGDKYAWLSWQTAVMLGGTVLLSLVFVLVERRAKDPVLPLWLFRSRTIVLAIGASLVIGIALYAASTFLSQYFQVAEGQSATRSGLLALPLILSLAVASTIGGRIITTTGRWKPVLVAGSLLITLGLGVLGLTRDGTAYWQLSIGMGLLGLGLGTTLQNLVLAVQNSVNPSELGSASSSVAFFRTLGGTIGVSALGAVLAGQVSRYTSDGLAALGVPAVSGGSSPRPADLPAPVRVVVEGAFGHATGNLFLYAAPLALIALVCILFIREVPLRTSNVVRDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVK6</accession>
    <name>U5VVK6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359192.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06860"/>
    <dbReference type="EnsemblBacteria" id="AGZ39661">
        <property type="protein sequence ID" value="AGZ39661"/>
        <property type="gene ID" value="AFR_06860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1401"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_332245_0_0_11"/>
    <dbReference type="OrthoDB" id="116882at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="861" mass="91515" checksum="87FAE1B0467A527B" modified="2014-01-22" version="1">MDSTVTEAVPQSLDSGPEGPGRGGLISVLRGHLDLFLNAGSLMATTAITSLFGFAYWWLAARSASAEAVGQASAAVSAMTLIGTIGMFGMGTMLISDLSAMRSRRWQLISTCLLVAGSASTVGGLIYVTLAHLAVPGLQNALGSTPATVLLVFGIALNAMTLVLDEALVGLLQGPMQLMRNAWFSVIKLALLGLLAVLPLTLTGGQLLLTWIAGMVASVAILGRALRRKGMIDSVRPQPSLLRGRGRATFDHNLLNLATYLPRAALPLVVTAVLSAEANASFYTAFMVLSFLAMVPGNVALTLFAVASGDKAALRSKVRVGLLICLGVGFPASLVVVFAAKPIMGLFGASYAESAGTALMILGLTYLPFVFHHFFLAISRVQGRVRGAGIFSVFAGVAELVAAWYGGSRGSLTELVTWVALVMAVETVLVAPTVLRVVFARTPLEENVMSTTSLTLHERAWLPLEYIRTVGPLTGVTAERLRAALIGLHAADPTHRAVSRLDRAGARWEHLSAEAFAGYVREAVTDLGDGPVDFDAMTRRLQAEPRAHHPVRILAGGGYVAMKVAHAYGDAGPVNTLLRELVRAAGEERAAVIGPSVRHRWALPKAWWKQFGTKPGRWKEGLSFARPPHTEDVPTRPWTADLTVVTARSADVLGKMRVWRDQYSPGVTTSAITFAAFTAALRELGLNPDLSGGTFLADARRYVDKGVSIDSNFCMGPYLSPPSLTDPQAIHTTLKAELATGRILTMMLLREGKLALTGTPGMPDPYPGEAPIDPRPRLTFSNQGRHDVLADLPWAADVPDRINQSVPTLNGPEGITLTTSEMGGVLHLEATFHASTYDPAVVARALQLVCTDPAALIMATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVU1</accession>
    <name>U5VVU1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ComE operon protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_07755</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07755"/>
    <dbReference type="EnsemblBacteria" id="AGZ39840">
        <property type="protein sequence ID" value="AGZ39840"/>
        <property type="gene ID" value="AFR_07755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1582"/>
    <dbReference type="eggNOG" id="COG1555">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052011_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="3">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="3">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="310" mass="31179" checksum="75CDF6F3CB23C910" modified="2014-01-22" version="1">MTLEPDDPGRPDEPPAPAPRAMTPEELAYRPAWAAAETSARRQIRPPSPYGSEPSDLSPPGVSPAGPSLTGRPSLGVDDLDSDAFEDPLTGAGAFDAAPRSRLGAFDPGKRGARALAAVAVIVVLVAAFLAWRARPRVDPVVPPVVDSVATDAPFADISGSTAASGPVEVVVAVGGKVRKPGLVQLPPGARVADALKAAGGANPGVDVAPLNLARKVTDGELIMVGVPLPPGAAGPAAPAGPGQPGAGPVNLNTATLADLDTLPGVGPVLAQRILDARDAQGGFKAVADLRKVQGIGTSRFDQLKDLVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVY2</accession>
    <name>U5VVY2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361182.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14205"/>
    <dbReference type="EnsemblBacteria" id="AGZ41123">
        <property type="protein sequence ID" value="AGZ41123"/>
        <property type="gene ID" value="AFR_14205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2882"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_0_1_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="23"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31401" checksum="B214F6CD1C968444" modified="2014-01-22" version="1">MMIASNGDVHLAADDLGGAGGDPLLLLMGLGVSRFYWPDGLVAALIGAGFHVACFDGRDSGGSTHFDDAPASDPLTGLLRRRPLAYTAEDMADDAVAVLDALGWQRAHLFGISQGGLVAQRTALRHPERVQTLTTYAAVPSDARGLAVLRYIRLPFLVRLARMRQPAGRDGDIAAGLELLRAVASPDYPFDEDDARLRVERELDAGLPSGVRDSRAQARQTSASWHGPRLRELSVPTLVLHGEKDPLCRPSAGRRIAALVPDARYVELPGTGHDLPREIWSTVVRELRALAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW29</accession>
    <name>U5VW29_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361242.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41183">
        <property type="protein sequence ID" value="AGZ41183"/>
        <property type="gene ID" value="AFR_14505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2945"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_0_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="35"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="295" mass="32581" checksum="1C57D893F04F449A" modified="2014-01-22" version="1">MDNRAEVREFLTTRRAKISPQQAGLPATGQRRVPGLRRSEVAALAGMSVEYYAKLERGALAGVSPGVLEAIARTLRLDDAERAHLLRLAHEADGSNAVLRPHRRPRQTQIRPSLQWSLDTITTGPAIVVNNRQDLLAANHLGRAMYSDVYADPSRPPNFARFTFLDSAARRFYPNFDVAADMTVANLRTAAGKDPHDKGLHDLVGELSTRSDDFRRRWGSHNVRTHGTGVKTFHHHAVGDLELAYESMDLRADPDLTMTLYAAEPASPTEDALRLLASWAATHGTDRGSVRPVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWA1</accession>
    <name>U5VWA1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359546.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08630"/>
    <dbReference type="EnsemblBacteria" id="AGZ40015">
        <property type="protein sequence ID" value="AGZ40015"/>
        <property type="gene ID" value="AFR_08630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1756"/>
    <dbReference type="eggNOG" id="ENOG5031VGD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2317138_0_0_11"/>
    <dbReference type="OrthoDB" id="2053120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="103" mass="11480" checksum="D875D5CFCFCE316C" modified="2014-01-22" version="1">MAAVIHQPKIAFDGARAFVRAAGTPAYDALATQMLSRLGTDWYGELANTRQRLLTASVESSDNDRFTADTETSKWRVRLEDLLRRRPDLATAVRELTEAVSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWG3</accession>
    <name>U5VWG3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hflX</name>
        <name evidence="10" type="ORF">AFR_14175</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361176.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14175"/>
    <dbReference type="EnsemblBacteria" id="AGZ41117">
        <property type="protein sequence ID" value="AGZ41117"/>
        <property type="gene ID" value="AFR_14175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2876"/>
    <dbReference type="eggNOG" id="COG2262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019597_1_0_11"/>
    <dbReference type="OrthoDB" id="1592033at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="PTHR10229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="9">
        <location>
            <begin position="221"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="227"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="252"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="273"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="339"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="363"/>
            <end position="365"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ41117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="399" mass="42773" checksum="CBBB6E81648399C0" modified="2014-01-22" version="1">MAGAWLLGAETRATADERPRAVLMAVVQDDRTDEGAPGTPASLRELERLADTDGLTVAETLVQSRDRPDPATYLGSGKAGELAAAVERCAADVVIADGELSPGQARNLQDKVGVPVVDRTALILDIFAGHARTSEGRLQVELAQIAYQLPRLQGAGRSMSRVGGGRVVGGAGMGVRGPGEMRLEIQRRRLRRRAAGLREQVARLGRRRERMQWRRTRNRVPSVAITGYTNAGKSALLNRLSGADALTQDVLFATLDPTVRRVTAQELRFTLTDTVGFVRNLPHQLIEAFRSTLEEVARADLALHVVDASAADALSQISTVHEVLREIGAGQVPEMLVLNKIDVASPETVSALRRVYLDALQVSALTGAGINELRDALAGRLQEREAQRSAGTSGTPSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX39</accession>
    <name>U5VX39_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_10005</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10005"/>
    <dbReference type="EnsemblBacteria" id="AGZ40290">
        <property type="protein sequence ID" value="AGZ40290"/>
        <property type="gene ID" value="AFR_10005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2039"/>
    <dbReference type="eggNOG" id="COG0772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029243_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018365">
        <property type="entry name" value="Cell_cycle_FtsW-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00428">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="10" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="451"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="484" mass="50997" checksum="06537125ED2A8637" modified="2014-01-22" version="1">MGEDKPDSRPVVVSPAATAQPAKAAAKPKSLSQQLLPAVHGLLARPLSSYYLLIASSGLLLLIGLTMVFSATSVKAYAQDGNAFSAIRNQLIFAVLGLVGFWVCQRLPSGTLRFLGKYVLGAAIVLLAILDLLMALKAVKLVDSPRLGPVHAELLWLYIGPVGVQPSELAKLGVVLWGADIIARKGASLGHWRELAMPLFPVVGLLFVLVGYNDLGTMLVLLALIIGLLWAAGVRLRVFAALGVLGLSGIGLLIAAASRGAGSGTDGAENYRLLRLTTFLKAPENCDLDLCYQLLQGRSAIYEGGWFGVGLGKGALKWNWLPAADNDFIYAVVAEELGVVGCAVILALFAVLAYTGFRIARRVEDPFRRLAATAITTWLVAQAVINIGGVVGLLPITGLPLPFISAGGSALVVTLAAIGMLASFARAEPDAARALHARPPARWVRLVWAPLPPLPPPRRPGPSRPSAQKTGNRAPANAGGDRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX93</accession>
    <name>U5VX93_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta_helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361648.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16455"/>
    <dbReference type="EnsemblBacteria" id="AGZ41573">
        <property type="protein sequence ID" value="AGZ41573"/>
        <property type="gene ID" value="AFR_16455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3337"/>
    <dbReference type="eggNOG" id="ENOG5032YH1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712959_0_0_11"/>
    <dbReference type="OrthoDB" id="1290413at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666173" description="Beta_helix domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Beta_helix" evidence="2">
        <location>
            <begin position="155"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="387" mass="40782" checksum="D9872F6C75376ED9" modified="2014-01-22" version="1">MFRILKAALVTAVVGLSTCLVTAAPAAAAGVQTIYMNAAGSDTQTGGTPGTAVKTLARVQQLVAAGPLDQDVEVRVHAGTYIAQGIVWTTYRPGHTISFMPDDYEYGEGLDGIAALPVFQNARASQSNRYITGPWFFACPGGPGQPLEDGGVSGLRFYYLKVTYYANAGISLDGSGGSTCGGKYAATSGPGQPSARGLDGNTIFGVRFNNIGNLYTGGTCADPDFTRCGYGGIVLTESSGNRIANNQFVNLRNSELSYIHAIYVTHKSSHNQFTANQVTNVSSDPVKIRDASNYNSFENNIFGANGFVRSSTPPAVHYLDEVSTTEGECSSFHNRFAYNDLGTYLVGSTENLPVWYLSPGGATWPGSTGCPALPAGETRLITAGNTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXA3</accession>
    <name>U5VXA3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_16505</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + 2 NAD(+) + UDP-alpha-D-glucose = 3 H(+) + 2 NADH + UDP-alpha-D-glucuronate</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16505"/>
    <dbReference type="EnsemblBacteria" id="AGZ41583">
        <property type="protein sequence ID" value="AGZ41583"/>
        <property type="gene ID" value="AFR_16505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3347"/>
    <dbReference type="eggNOG" id="COG1004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_2_11"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="12">
        <location>
            <begin position="336"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="156"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="262"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="454"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ41583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="480" mass="51005" checksum="D224D0F545A461E1" modified="2014-01-22" version="1">MTLPRLTFVGTGYLGATYAVCFAELGYEVLGFDVDTAKIAALSDGVVPFHEPGLGVLLRRNLAAGRLRFTTSYAETADFGDIHFVCVGTPQRADDTGADLSYVESAVTGLARRLTRKALIVGKSTVPVGTAEWVQRLVDKHAPAGLGAEVAWSPEFLQEGFAVQDVLRPTRIVAGVRSDWAATMLSAAHRGVLDLAAREQREVPVVVTDFATAELVKVAANAFLATKISFINAMAEVCEAAGADVDQLARAIGHDPRIGSRFLRAGAGFGGGCLPKDLRAFQARAQELGVGESLRFLHEVDLINQRSRSRVLRRAAELLDCPTGPAGPDFGNTRVAVLGATFKPHSDDVRDSPALAIAAALSASGADVHVYDPEGMGNAARVHPNLIFEPGLEEAVRGSELVCVLTEWPEFSAADPVHLGRLAAGRLVVDGRNCLDVAAWTAAGWRYRGMGRHAEPATDLRPRPVRPAIPRPANRSVAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXU2</accession>
    <name>U5VXU2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11010"/>
    <dbReference type="EnsemblBacteria" id="AGZ40491">
        <property type="protein sequence ID" value="AGZ40491"/>
        <property type="gene ID" value="AFR_11010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2242"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054927_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="50"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="337" mass="36859" checksum="E8B04211CB349EB0" modified="2014-01-22" version="1">MTTADLPPATKDASTARAEAVLSRLQLLVTRKLDGLLQGDYAGLLPGPGSEAGESREYRPGDDVRRMDWPVTARTTLPHVRRTVADRELETWMAVDLSASLDFGTAAMLKRDLVLAAATAMAHLTVRGGNRIGAVVGTGSGVVQREQRGFPWRRRPERAEQAPESAPVVRMPARPGRKEAQGMLRAIARTRIRPGRTDLGALIDLLNRPPRRRGVAVVISDFMAPVDGWSRPIRKLGVRHDVLAIEVVDPRELELPDVGVLTLADPETGALHEVQTADPELRARYAEAAGNQRGEIARALRAAGAAHLRLRTDTDWLLDVVRFVAAQRHARTRGTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY87</accession>
    <name>U5VY87_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative anti-sigma regulatory factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360729.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11905"/>
    <dbReference type="EnsemblBacteria" id="AGZ40670">
        <property type="protein sequence ID" value="AGZ40670"/>
        <property type="gene ID" value="AFR_11905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2424"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133205_1_0_11"/>
    <dbReference type="OrthoDB" id="1832160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HATPase_c" evidence="1">
        <location>
            <begin position="12"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13390" checksum="766054FFA456407C" modified="2014-01-22" version="1">MTDLPATMTYSEVGDLKAVRDFVAARASSLGLPPVRVELLILAVSELTTNTLQHTDGAGRIHVWVRAGQLICDVVDSGPMRTFGRDMPAAGIVGGRGLAIVERICDDVETFPVEGGTMVRIRLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYC1</accession>
    <name>U5VYC1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA topoisomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_17495</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17495"/>
    <dbReference type="EnsemblBacteria" id="AGZ41777">
        <property type="protein sequence ID" value="AGZ41777"/>
        <property type="gene ID" value="AFR_17495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3546"/>
    <dbReference type="eggNOG" id="COG3569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046978_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.66.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035447">
        <property type="entry name" value="DNA_topo_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55869">
        <property type="entry name" value="SSF55869"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Topoisom_I" evidence="5">
        <location>
            <begin position="76"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="35028" checksum="D852B3833A544663" modified="2014-01-22" version="1">MTGPGLSRRRSGKGFRYLGPDGQAVKDAGTLDRIKALVIPPAWEEVWISPDPQGHIQATGKDAAGRKQYLYHRVWRSTQDEAKFDHALEVAGHLPELRSRLCTDLSGRGLTRARVLAAVVRLLDMGMFRVGSDQYARRDEDPSYGLSTLLPEHVRVRGGCVVLEFTGKAGVEHANPVEDGEVCQVLRDLKRRRRGEQRLFAYWDGSGRRWREIRADDINDYLREATGEEMTAKDFRTWHGTLRAAEELAAAGPQETKTGRKKVVTHAVKEVAGLLGNTPAVARASYIDPRLIEKYEDGEVTGASEEEVLDLLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYF2</accession>
    <name>U5VYF2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12270"/>
    <dbReference type="EnsemblBacteria" id="AGZ40740">
        <property type="protein sequence ID" value="AGZ40740"/>
        <property type="gene ID" value="AFR_12270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2493"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013364_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013595">
        <property type="entry name" value="Pept_S33_TAP-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08386">
        <property type="entry name" value="Abhydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666206" evidence="2">
        <location>
            <begin position="28"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="119"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Abhydrolase_4" evidence="4">
        <location>
            <begin position="456"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="550" mass="58005" checksum="CB7EB8E642822A4B" modified="2014-01-22" version="1">MSRVLPRILAALVTAGAGLVAVPWAHAATSPVQHSVDAPVSQDRFVGVPASPLPAEAATRTTRSVVSKLRWGSCPTDAPAPQQCATLQVPLDYRHPHGRAISVEVSRIPAADPAKRKGVLMLNGGGPSANLDVPTAFGTLLPAEVRDSYDLVAFDPRGIGYSTPMSCGRGADELIRDLQLSLLSFPAADGSIGTNVSFAKRMAKQCAANSGDLLKHITTANVARDMDQIRRALGERTVSYYGLSWGTYLGSLYRALFPRTVDRMVIDSSVDPNVRGYADFRTFSGAMEDRWPDLAAFGVANAGSVRLGTTPRQVRKNYLALTARLDHHPVMLADTPAPINGNFVRLFTWLLSYSDAALIGTEEAPLPQMAQLWRAAGAVAAGHAIAADGAFLAGLANGLVASGTLPNVPQDNLLSVGWAYSCGDKAWPRKVGTYARNVAADRATFPLTAGAPANIAPCSAWAVQPVGPEPTVEPIGRRNVLILQNYRDPSTPLRGARGMRRAMGSDAVLVTVDASGHGVLIHPRPNACAIGALTSYLTTGALPATDKTCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ59</accession>
    <name>U5VZ59_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361074.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13645"/>
    <dbReference type="EnsemblBacteria" id="AGZ41015">
        <property type="protein sequence ID" value="AGZ41015"/>
        <property type="gene ID" value="AFR_13645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2771"/>
    <dbReference type="HOGENOM" id="CLU_2766530_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="69" mass="7173" checksum="C6DCBC285FF6869D" modified="2014-01-22" version="1">MSANGDTDPTSEPVGPGRPSDELEHWLTDLRTEVSADPSGWIDKDSGGEPAGVEEAAEPSTGGRHRAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZB5</accession>
    <name>U5VZB5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Multidrug export protein MepA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_13700</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13700"/>
    <dbReference type="EnsemblBacteria" id="AGZ41026">
        <property type="protein sequence ID" value="AGZ41026"/>
        <property type="gene ID" value="AFR_13700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2782"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13143">
        <property type="entry name" value="MATE_MepA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045070">
        <property type="entry name" value="MATE_MepA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="427" mass="44133" checksum="200B48238D94B1D3" modified="2014-01-22" version="1">MLWHACTQTTLSVGVYGIYALTNAWFVARGVGPDAMAAVNLVAPVLLVLGAVTTTVGIGGASLVSRHLGAGDTAGAARAAGNAFAIFWAAAVTVCVAGLLAIEPLLTLLGARGDIRADARDYAVIILAGALVSTGFSSLVRAEGRMRFSTLLWVVPVVVQITLDPLLIFGFDLGVRGAALGTVGGQSVSAAMSIWFFFLQRRRPYRITRTDLIPYGHTVRALLSVGAPSFLAGSGATLLALLVNNTLVHAGGAVALAAYAVCSRIRTFVSMPQLGISQGLQPIVGYNAGRRLFDRVRRAETLSLRATLTYGVLTTTAVVLFAGPLVGIFLEEGPTAAAAREALRILAIGLAVAGVAPLVSACFQSLGRATPSYVISIGTLVVVKAPLVLLLSPAGTTAVWIALAAGDIISALIAIVILRRLRPRGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZG0</accession>
    <name>U5VZG0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_20580</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362760.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20580"/>
    <dbReference type="EnsemblBacteria" id="AGZ42388">
        <property type="protein sequence ID" value="AGZ42388"/>
        <property type="gene ID" value="AFR_20580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4173"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="26232" checksum="993B15C78FE31A27" modified="2014-01-22" version="1">MTRPVLDVRDLTKIYGTGAATVHALRGVTLTVDRGDYVAIMGSSGSGKSTLMNILGALDVPTAGTYRLDGVDVSRLADRQLALARNRLIGFIFQAFNLIPRTTAVANVELPLAYAGVKSRQRRERAMAALETVGLADRARHEPNQLSGGQQQRVAVARALVTEPALLLADEPTGNLDTRSTADVLEVFDRLSAAGRTIVLITHEPEVGDRTKRLVRLVDGRIVTDVRQSPLDRVPVGAPGRHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZI8</accession>
    <name>U5VZI8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonate-semialdehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_20705</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362785.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20705"/>
    <dbReference type="EnsemblBacteria" id="AGZ42413">
        <property type="protein sequence ID" value="AGZ42413"/>
        <property type="gene ID" value="AFR_20705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4198"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_10_11"/>
    <dbReference type="OrthoDB" id="490746at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="PTHR43866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="3">
        <location>
            <begin position="19"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="498" mass="52564" checksum="5C0D8D85CB572BF3" modified="2014-01-22" version="1">MTTIEHWIGGSSTAGAGSRRAPVFNPATGQQQHEVVLADTGDVEAAVAAAKAAFTTWGQASLSARTKIMFNFRELVNANVDRLAELVSDEHGKVLSDAAGEVQRGLEVVEFACGIPQLLKGDYSDQASTGVDVFSFREPLGVCAGITPFNFPVMVPMWMYPVAIACGNTFVLKPSERDPSASNFIADLWRQAGLPDGVFNVVHGDKTAVDALLDHPDVAAVSFVGSTPIARYIHEKASATGKRVQALGGAKNHAIILPDADLEYAANHLSAAAFGSAGERCMAISAAVAVGGAGDPLMDILTRKANDVVVGSGRDPKSEMGPVVTAAARDRIESLIGTGEQQGAKVLVDGRGRRVPGFEEGFFVGPTVIDQVDTSMDVYTEEIFGPVLSVVRSESVEAAIDLINANPYGNGTAIFTNSGEAARRFQRGVHVGMIGINVPIPVPMAYYSFGGWKDSLFGDKHIHGPEGVSFYTRGKVVTSRWPHVEAAHGASMHFPTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZK0</accession>
    <name>U5VZK0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361125.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13900"/>
    <dbReference type="EnsemblBacteria" id="AGZ41066">
        <property type="protein sequence ID" value="AGZ41066"/>
        <property type="gene ID" value="AFR_13900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2820"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_1_2_11"/>
    <dbReference type="OrthoDB" id="2089619at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041467">
        <property type="entry name" value="Sco4008_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17926">
        <property type="entry name" value="TetR_C_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="20207" checksum="0B052B8D176A1643" modified="2014-01-22" version="1">MDRSQASRRRLLDAGAIEFAAYGVAGARVDRISVDAKVSKAQIYSYYAGKENLFDAVLEDQVNDILDRAPLTADLPDYAVRLYDAYLEHPQLVRLATWARLERVPSGPLFPQADETAKTEVIAAGQQAGRIRSDMSAADIHSMVIAMAMTWSPSSLTYTATPADAAGDHERRRAGLAAAVQGAFMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZR8</accession>
    <name>U5VZR8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative macrolide-specific efflux protein macA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_20560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20560"/>
    <dbReference type="EnsemblBacteria" id="AGZ42384">
        <property type="protein sequence ID" value="AGZ42384"/>
        <property type="gene ID" value="AFR_20560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4169"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_14_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666245" evidence="3">
        <location>
            <begin position="32"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="4">
        <location>
            <begin position="58"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="382" mass="38189" checksum="4B33E821525A8F73" modified="2014-01-22" version="1">MWAAGIVVVLLLGAGAARLVTAGGNPKPAVAATVAVDRGAVTLEVATTGTLEPAQTRSLSFAVAGTVESVKVRAGTLVTPGQTLATIDDTDAAAAVDSARDTLTEAQDALTEAREAPTSSCGVVPAAWTSPSTQPSPSRSSAPAETPAPARTTAPARTSAPARTSAPARGAAPTTCAAKGGRADPVLSAQQRVNQAEVALANAEDRLDGTTITAPIAGRILSVSGKVGSQIGAGTAFLTLADVYDMQISADFPEADADRLAVKQKAAVTLADRAGKIFGATVVQVDPAGTNDGTMVRYGVLLSFDDAPDDLLVGQSAGVRVTTGSAADVLRVPSTAVHDVTGGSGTVLRDGARLEVGVGLRGDLYTEITSGLAEGAAVVRSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZV9</accession>
    <name>U5VZV9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_20175</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362679.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20175"/>
    <dbReference type="EnsemblBacteria" id="AGZ42307">
        <property type="protein sequence ID" value="AGZ42307"/>
        <property type="gene ID" value="AFR_20175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4092"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019414_0_1_11"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EHN" evidence="3">
        <location>
            <begin position="4"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="39944" checksum="BEA5CE8F3AF77B4E" modified="2014-01-22" version="1">MNDIEPYRVHVPQADLDDLADRLGRARWPDELPGVGWDYGIPLERVRELAERWRTTYDWRVHEAALNAYPQFVTEIDGQRIHFLHVPSANPSALPLVLVHGWPGSVLEFLDLIEPLTDDFHLVIPSIPGFGFSGPTHEKGWGLERIARAFAELMSRLGYERFGAHGGDWGSGVARALGGVAPDRVVGVHVNYLPTPGPVDGLSGQDRERAERIAAFAANRPGYQVLQATRPQTIAYALTDSPIGQLAWIAEKFTEWADPASSISDDVLLSDVSIYWFTRTAGSSARLAKESGRGTPCPVPLGVAVLPHDIVQSVRPLAERSFDIRHWTELPRGGHFAALEVPQDLAADIAAFFQLQGAVTSSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZX3</accession>
    <name>U5VZX3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mannosyl-D-glycerate transport/metabolism system repressor mngR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20250"/>
    <dbReference type="EnsemblBacteria" id="AGZ42322">
        <property type="protein sequence ID" value="AGZ42322"/>
        <property type="gene ID" value="AFR_20250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4106"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_2_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21866" checksum="47F4FE33D3940628" modified="2014-01-22" version="1">MTLRQALRQLEDDGLLSQQPGRGTFVASPKATYRLDSLRGLAEDLRAQGRTVSTEILGQTLRRPPAWVAAQLGPGRVLRLERLRLLAGRPAVHQLSWVRSPVGDELRDTDLSGTSLYAAIAEHGVVVHRASEVLRPEVLTAPVADLLRQGAGTPVFVSDRITYGLDDQPIVVDRATILGTVMEVRTERAATGLSMQWTSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0F9</accession>
    <name>U5W0F9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyridoxal-dependent decarboxylase exosortase system type 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_15940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361529.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15940"/>
    <dbReference type="EnsemblBacteria" id="AGZ41470">
        <property type="protein sequence ID" value="AGZ41470"/>
        <property type="gene ID" value="AFR_15940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3236"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_3_11"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017530">
        <property type="entry name" value="DCO2ase_PEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03099">
        <property type="entry name" value="dCO2ase_PEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="3">
        <location>
            <begin position="52"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="2">
        <location>
            <begin position="292"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="408" mass="43162" checksum="926D3CF5816736E3" modified="2014-01-22" version="1">MTQHPTAAAFARIDGQLAVGGVPVGRLAERVGSTPFFAYDRGLLTGRVGLLRATLPADVQLSYAIKANPMPAVVQHLSGLVDAFDVASALEMRTALDSTMPATRVSFAGPGKTDAELRQAVAAGVTIELESENEARRIGLAGAALGIRPRVAVRVNPDFAVKGSGMRMGGGPQQFGVDAERVPALLKDLAGTDLEVLGFHVFAGSQNLRADIICEAQQRTVELLLRLAGDAPEPVRYLNLGGGFGIPYTDRDVPLDLAMIGDNLTTLMRDEVRPRLPEARVVVELGRYLVGECGVYVTRVVDRKESRGKTYLVVDGGMHHQLAASGNFGQVIRRNYPIAVANRIAEEPAEQVSVVGSLCTPLDLLGDNVTLPEAGVGDLVVLYQAGAYGLTASPTAFLSHPAPAEVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0H1</accession>
    <name>U5W0H1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363089.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22240"/>
    <dbReference type="EnsemblBacteria" id="AGZ42718">
        <property type="protein sequence ID" value="AGZ42718"/>
        <property type="gene ID" value="AFR_22240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4508"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1123615_0_0_11"/>
    <dbReference type="OrthoDB" id="1694750at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="24733" checksum="C8C8191DC869CD94" modified="2014-01-22" version="1">MTEPSFDEPEGINPRGTVIVAAGRGETGQAYARLGRRLAADGYRVRVLPDVTTDLTASLAHAEKVLVDDALPGPKVIAGSDTGALFALALAGRHVPGLDAVILAGIPVPVATGGSVDLIGWEAEVEARTACPNHQRLLGDGGARPGALFSAPIPTELIELAGDGSGGLPALGLHGSADLVSPLGQARDLYPSGGSARLVAIEGGRHDVLNDVTHRTVAALIVLFLERLRLGTDLPLIARPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0N1</accession>
    <name>U5W0N1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Type I phosphodiesterase/nucleotide pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363251.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22640"/>
    <dbReference type="EnsemblBacteria" id="AGZ42798">
        <property type="protein sequence ID" value="AGZ42798"/>
        <property type="gene ID" value="AFR_22640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4589"/>
    <dbReference type="eggNOG" id="COG1524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047974_0_0_11"/>
    <dbReference type="OrthoDB" id="191097at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023116">
        <property type="entry name" value="Phosphonoacetate_hydro_insert"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="460" mass="50092" checksum="585F0BE742D2A67E" modified="2014-01-22" version="1">MKPVVVLDVAGLTPRLLDHMPRLARLAGTGFRATLGTVLPAVTCSVQSTILTGALPAEHGIVGNGWYFRDLGEVLLWRQSNALVGGDKLWHAARRQQPGYTVANVCWWYAMGADVNWTVTPRPVYHADGRKDPDCYTDPPELHDELTAKLGTFPLFSYWGANAGLPSSAWIVKAAEQIMADHQPDLTLVYVPHLDYDLQRYGPSAPQAAAAAAELDVTLGPLLDAAAARDATVVVLSEYGITDVSQPVDINRLLRSEGLLNVYTQAGMEYLDPWTSRAFAVADHQIAHVYVRDPADLEAVTKLCEGLPGVAEVLDATAQHEYGLDHERSGDLVLVAEPYAWFTYYYWLDDAKAPDFARLVEIHRKPGYDPAELFFDPAGPGAAKGRAGVALLRKKLGMRYMMSVVGLDAGARAVRGSHGRLPSDERDAPVLICSDPAFARDRVEATDVKDLLLRLAGLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0U7</accession>
    <name>U5W0U7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23065"/>
    <dbReference type="EnsemblBacteria" id="AGZ42883">
        <property type="protein sequence ID" value="AGZ42883"/>
        <property type="gene ID" value="AFR_23065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4674"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_12_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="150" mass="16428" checksum="739683B1F785891D" modified="2014-01-22" version="1">MVAALCALRPAAVWSSPYRRAIQTVQPAADVLGLPVRTLGELREWDDGLAYTDDWERHYVQSWADPSYARPGGESLDQLSARSVAVVRRLVREHPGQVVLVGGHGTIISRVLAGFGVPVGWETVKRMPMPAIFRLRFAGPDTRPEVSGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1B8</accession>
    <name>U5W1B8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17410"/>
    <dbReference type="EnsemblBacteria" id="AGZ41760">
        <property type="protein sequence ID" value="AGZ41760"/>
        <property type="gene ID" value="AFR_17410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3529"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4995">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009955_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024983">
        <property type="entry name" value="CHAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12770">
        <property type="entry name" value="CHAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHAT" evidence="1">
        <location>
            <begin position="590"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="832" mass="88210" checksum="02B4B7F2E347FB23" modified="2014-01-22" version="1">MARAAGDIEALVVGLRAQAWARHVALDNDGARRLIEQAVRLAESHGLPGRLGDLLVTRSVALHELGRYDAAARDLRRAEPLVAPEERPDLHLQLAILDHNRGRVRAAEQAYQEVLADPACPPVVWVKAANNLANALTQLGRPHEALDHLAHAAELCRELSPRLLAVIANSRAWSTFHAGQLAASVRRFEEAGRLHAAAGLPLGEHQLEYAEVLNDLRLLDEAMAAARSAEVEFDRHGARLMAPEARLRCARLALELGDCATALTDAAAAADDFRRQRRPAWAARAAVTEAQARAACDGHSPEVLRRLGGAAATLQRLGLRTEAVYGHLAAGRTALALGQRPAARRALTLAGDLARDQSLLVRLRGNLARALLAEAEGTGDVVSECSRGLRDLARHRAALPSLELRVLAAGHGAELGELGLRRLLPTASAGRILGWLERTRTAALLRVQPPATGVDQDVIALRGVEQELRTARLERGEEPPALLTRQSALETRIRRRSWAGEDVGGEAAALASAADLRAALDGQWLAEFAVVDGQILAVVVEPRRTRMVELGPIAPVLQEADAAAFGLRRLLHGTRFSAAARTAAQSAFDALSRQLIVPLGVPADVPLVVVPSGELLRVLWSPLHPAPVSVTPSGGLWARARRTRTDSPVRVAVIAGPDLPGAIEEAHAVAAGHKEVVTLLPPDSTVDAAVELIKGADLAHLACHGLFRSDSPLFSALELSDGPLTLYEMLARGVAPHRVVLASCDSGTQQPYGGNEMLGFVSALMSNGTAGLVAAEVPIPDGACAGPMTVLHERIGQGDTLATAVWHARSALADGTPEDYVAWSGLTAYGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1H4</accession>
    <name>U5W1H4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative serine/threonine protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17760"/>
    <dbReference type="EnsemblBacteria" id="AGZ41830">
        <property type="protein sequence ID" value="AGZ41830"/>
        <property type="gene ID" value="AFR_17760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3601"/>
    <dbReference type="eggNOG" id="COG0639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055447_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="20"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="26133" checksum="3B00EE44F93B1B7F" modified="2014-01-22" version="1">MREALREAGLTGADGRWAGQDAQLWLLGDYVDRGPDGIGVVDDIRRLTGEARATGGEVGALLGNHEVQLLAAHRFGHAPVAGWGRPGGFRELWARYGGQAGDLRRLTPEHLAWITALPAMALVDGHLLMHSDTTRYLEFGRSVEAVNAAVAEALRGTDVDDWWEFVRRISGRGTFRADGGEDEAAVMLGVLGGEVIAHGHSTLIQHFGVAAGDVREAVRYAGGRVVAVDGGVFEGGRLLLTRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1H8</accession>
    <name>U5W1H8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363638.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23900"/>
    <dbReference type="EnsemblBacteria" id="AGZ43048">
        <property type="protein sequence ID" value="AGZ43048"/>
        <property type="gene ID" value="AFR_23900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4841"/>
    <dbReference type="eggNOG" id="ENOG5033ZN1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093862_0_0_11"/>
    <dbReference type="OrthoDB" id="1407370at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665969" evidence="2">
        <location>
            <begin position="23"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="22154" checksum="89A96C004D9DE863" modified="2014-01-22" version="1">MGAVGVAVLLLGALHVVPPSSAVSPYRRTISEYALTDAGPVFNVAVLILAAGSVATLLAALGAGLFPPLSGGAVSLVLWSAALAVVVYFPKHNWAVGPSAGGTIHRIASVVAFLSLPIGALLIGRAWRRHKRWRAHATWTLGLGVWSLLSFLPIVGAIVAQPWTDVAWWRAIPLGAVERVLTISEVGIVVVLAAWAFAAARVRNGAAREALVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1N2</accession>
    <name>U5W1N2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate-binding protein PstS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18060</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of the ABC transporter complex PstSACB involved in phosphate import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362254.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18060"/>
    <dbReference type="EnsemblBacteria" id="AGZ41890">
        <property type="protein sequence ID" value="AGZ41890"/>
        <property type="gene ID" value="AFR_18060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3665"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034528_0_0_11"/>
    <dbReference type="OrthoDB" id="1222928at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665445" description="Phosphate-binding protein PstS" evidence="5">
        <location>
            <begin position="20"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="6">
        <location>
            <begin position="28"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="4">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="4">
        <location>
            <begin position="173"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR002756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR002756-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="345" mass="34442" checksum="94C5841E3D49FBA8" modified="2014-01-22" version="1">MRFLPVLLVALVAGCGTSAATATGAEGISCAAGSITAQGSSAQTTVVSAWIKGYQIACPEATIAYASTGSGAGVRAFGAGTGDFVGTDSVLSGEGRRTVGARCPAVHLPLVVGPIALAYNVAGVGGLQLRPATVAKIFAGVVTVWNDPAVVADNPGVTLPSTKIRTVHRKDNSGTTDNFTRFLTAAGGADWKFGAGSVWSAPGGSAEQGSNGVATAIARTDGAIGYVEGSYARFHQLATARIGNGAGEFAVLTDEAAALTIAGARVSGTGGDLRLTVDHRVAAPGAYPIVLVTYEVVCGKGSPALVRSFLAYAASPAGQAAAARLGYAPLPEKLRAQVVAAINRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Q8</accession>
    <name>U5W1Q8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18210</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362293.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18210"/>
    <dbReference type="EnsemblBacteria" id="AGZ41920">
        <property type="protein sequence ID" value="AGZ41920"/>
        <property type="gene ID" value="AFR_18210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3696"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_11"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665597" evidence="4">
        <location>
            <begin position="44"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="216"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="273"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="531" mass="54442" checksum="9352F441BD765917" modified="2014-01-22" version="1">MAAPRSLNDLSVNIKVVAAVGAAALVALVIGSMGLLSLSDASASAQTIYRDNVASIKAVGQLKAAVAQTRVDLANQALSQDEAATTRFAQGFAADLQTFDAAMAAYRAGGPAADPAVIDELEQTWRAYAGIARDKMLPAGTRNDLVGWAKIRDTETVPLLKEVYDTVAALDAMETDDAAANAAAAKSGYESSRLTSIVVLSVGLVIALGLGVLVARKIVQSLARVRNVCDGLAEGDLTRTTGLTSLDEPGQMGRALDTAMVRLRETISTIDGSAATLASATEQMTGTAAQIAASAEETTVQAQAVSAAAEQVSRSVDTVSSGSEEMGASIREISQNATEAARVAEEAVSVTAETSATMGKLGESSAEIGNVIKVITSIAEQTNLLALNATIEAARAGEMGKGFAVVASEVKDLAQETARATEDISRRVQAIQSDTSGAVTAIEGISAVIARISDFQTTIASAVEEQTATTAEMNRSVSEAAGGTGEIAQNITGVAEAARLTSQGVGETRQATAEMARMSTELSGLVAAFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1R4</accession>
    <name>U5W1R4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362303.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18260"/>
    <dbReference type="EnsemblBacteria" id="AGZ41930">
        <property type="protein sequence ID" value="AGZ41930"/>
        <property type="gene ID" value="AFR_18260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3706"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_5_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="26687" checksum="D8BF6DA3434F3738" modified="2014-01-22" version="1">MATILVTGSSDGIGLQTARTLIEAGHRVILHARSESRAEGLPSEAAGVVIGDLTSLDETKALAAAAGACDVVIHNAGVGGGQSEPVLTGDGVERIFQVNVLAPYVLTALVPWPARLVYLTSGLQSGGEVHFDDLSWRSRRWDGMQAYSDSKLYDVMLAFAVARRRPGTLSNTVDPGWIRTRMGGAGATGELSEGADTPIWLATSDEPAAKVTGRYFNRRQELRANPLAYREDLQEELLAACAELSGVKLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1X0</accession>
    <name>U5W1X0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363689.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43099">
        <property type="protein sequence ID" value="AGZ43099"/>
        <property type="gene ID" value="AFR_24155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4893"/>
    <dbReference type="eggNOG" id="ENOG5032X7T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097423_0_0_11"/>
    <dbReference type="OrthoDB" id="1846417at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="22959" checksum="E6C53C01016E73C3" modified="2014-01-22" version="1">MAETTVVLGRKRRHWPAVAGATVVVVLAVTAGLAVHGRDDAAPPAAFAVPSVVPSVPVSVAPVDGRLGDVVATGLPASGGQTWVLYFTPIDWDHTPGIRMGLSIGERDARGTIEEALNIAVTSGSDRTAGFHPMQAPMQLEDRVVQPAFGYYVGRPARITATFDGVTTVNAVLAPWSADPDVTIFWFDPAAGAVEEISQLGAFDAAGARLPDGIIKASYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W263</accession>
    <name>U5W263_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport system inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_25285</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363914.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25285"/>
    <dbReference type="EnsemblBacteria" id="AGZ43323">
        <property type="protein sequence ID" value="AGZ43323"/>
        <property type="gene ID" value="AFR_25285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5124"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_11"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="96"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="325" mass="35726" checksum="EAFD67823F750037" modified="2014-01-22" version="1">MTTVRPAQSRLRAVPPGPADRQGPHGRRHSENLSGYAFLSPWLLGLFAITAIPMLLSLYLSFTDYDVLTPLSEASWVGWANYERMFTGDPSYWHAVRVTLTFALVAVPLKLAAALGVALLLNRAIRGIGLFRGLFYLPSLLGGSVALAIVWVNMFNRDGAFNSFLGLFGVEGAPWVNDPDWALQTLILLAVWQFGAPMVIFLAGLKQVPVELYEAASVDGAGAWRQFRNVTLPMLSPVIFFNLVLETINGFQGFTSAFVLSNGTGGPVDSTLMYTLDLYIKGFVELDMGYASAMAWVFLLAIGLITVVLFRTGRFWVHYSDGEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W287</accession>
    <name>U5W287_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363834.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43244">
        <property type="protein sequence ID" value="AGZ43244"/>
        <property type="gene ID" value="AFR_24890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5042"/>
    <dbReference type="eggNOG" id="COG4122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079870_0_0_11"/>
    <dbReference type="OrthoDB" id="1120353at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="43"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="22723" checksum="E6E8115AD68224D9" modified="2014-01-22" version="1">MDWAAWHDDYDDPESALSRRLAEVQGVVRAALDRAPDGPLTALSMCAGQGRDLIGVLATHPRGHDVRARLVELDPRSTAVAEANARAAGLDGVEIVQADAGLMRHYRDLAPADLVLACGVFGNVTDADVLRTVEACAQLCREGGTVVWTRHRRPPDLVPVIRDWFSARGFELVHGGESGVGAHRLVHPPKPLTSADRIFSFVAEKQLRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2H5</accession>
    <name>U5W2H5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362479.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42106">
        <property type="protein sequence ID" value="AGZ42106"/>
        <property type="gene ID" value="AFR_19170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3894"/>
    <dbReference type="eggNOG" id="ENOG5031ZVT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_320716_0_0_11"/>
    <dbReference type="OrthoDB" id="1262187at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="912" mass="97506" checksum="BA12888619A132E1" modified="2014-01-22" version="1">MEVLLAGGIRPKPDHRVHAVARTALLPGTPVAVGPADEGPANALARLHALVAAGGVIAAGAGVDLGDGFRSARIDGGVGDRRDAVLAALSVPAFADHLGAPTALLVALFGPFATRPLGTVTLETIAAGRWPILRYAVACSDLLGPEQLVQLLALRAPEGVDPFPAGLPSVVATQLRRVTAGFSAPRRLRVLTDLWEQACGSSADRARRERIRGSQGRHDRYEELFERRRRHESDLITAALHRHFCAEPSLADVALWSPPAQVWVARADRIRDDAFAATVLARVAATAAEAGPEEALRRHQHEIAAGAATLSKRAASAASRAVPGLTGHPARPAVYLRDIHERIPVGVPLSPKRVVFLRDRFALARDYGQVALAACNLFLTGNPESSADGRDARSIWLRTGLRPWREQVGYFSPDRLPGWDLEPEDGAEPLGRRPADTETVADLLWYAELADAVARLNGHAAARLERERHQLFPDTNPAPEQEEPATVADFAAGAAQLAELGGAVPPRARTWTEVVAGLHQVVEAATAQSGRFVVPPAVDAFEGQLLPGSGMRMEIARTALQLVGWAGYMGNCIAEEPYPEDAAKGRVMLVALRGPDGRIRANVEVRLTGRGWRLAQIQGRFNDDPDPELRRSTEEWVASLPRTVVPPPADSVPEPAPARRPARPSPARRIVADLGDQLGDLAAGTVGPAPVLAALIDAPDGPEALVALRRSSPATLLRAVRRALAGPVTLDELWAASARRPLAEAVAALSDEEVRDRLAPLTVDAPLPGALRKLARLPRIAPARTADQVAIRVRAALGTLIRIDAPELSAAMTSRPHAELLRAGALAVTSWGGLDGNRPVIAVMARRRIRVPGYPQTTLRDDLWQQAWPGAEELGAARDVFWDAIAAKGLLVPASWLEPAGWPALWARAHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2I3</accession>
    <name>U5W2I3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363812.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24780"/>
    <dbReference type="EnsemblBacteria" id="AGZ43222">
        <property type="protein sequence ID" value="AGZ43222"/>
        <property type="gene ID" value="AFR_24780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5020"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098986_0_0_11"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="24961" checksum="EBCA209C537A27E4" modified="2014-01-22" version="1">MIRRIKACLNGDRAQGVPVTAAEVARAAAEAVAAGAEAIHVHPRDDHGAEFLAAADIGAAVAAVRGACPGTPVGVSTGLWITGGDVARRMAAVGGWAGLPVDRRPDFASVNVSEDGFADLARVLRGSGIAVEAGVWSAGEVGTLLGVPVDRILIEVLHTPAADAVASADTILERLDRLGVSGPRLLHGEEEACWPLIRHAGRLGLPTRIGLEDTLDLDDGSPATGNAELVARALEIWAKARLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2U6</accession>
    <name>U5W2U6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">zf-CGNR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362682.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20190"/>
    <dbReference type="EnsemblBacteria" id="AGZ42310">
        <property type="protein sequence ID" value="AGZ42310"/>
        <property type="gene ID" value="AFR_20190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4095"/>
    <dbReference type="eggNOG" id="COG5516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087298_0_1_11"/>
    <dbReference type="OrthoDB" id="1478254at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010852">
        <property type="entry name" value="ABATE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023286">
        <property type="entry name" value="ABATE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021005">
        <property type="entry name" value="Znf_CGNR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35525">
        <property type="entry name" value="PTHR35525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11706">
        <property type="entry name" value="zf-CGNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160904">
        <property type="entry name" value="SSF160904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-CGNR" evidence="1">
        <location>
            <begin position="111"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="160" mass="17563" checksum="AEF0081568CD3DAA" modified="2014-01-22" version="1">MTDEDLLLWMLNSTPVIDGRRTDRLGDPAARGGTGTGAELTAVRTARDRLQGVVRGDRPAADLAPLLEGVHRSPVIGDTGLEWQLDAPADRLLAVRAVLAWAELHEQHPGRLRACANDECRLFLLDRSRAGTARWCSMATCGNRMKARRHQGRYGRIAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W377</accession>
    <name>U5W377_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364368.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26460"/>
    <dbReference type="EnsemblBacteria" id="AGZ43554">
        <property type="protein sequence ID" value="AGZ43554"/>
        <property type="gene ID" value="AFR_26460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5364"/>
    <dbReference type="eggNOG" id="COG4226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105073_0_0_11"/>
    <dbReference type="OrthoDB" id="1988756at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008651">
        <property type="entry name" value="Uncharacterised_HicB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05534">
        <property type="entry name" value="HicB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="16815" checksum="DC4BEFE3E46B910C" modified="2014-01-22" version="1">MDLTPYLESLRADLSAAAAPGGPETVRAAELLGHAIEPSARLALLQALSDATAEITTRLNGPVVDVRLRGREADLVVSHTEPGPDPIAPPTAAPADGGDLARLTLRMPESLKTHVEQAAAAEGISVNAWLVRAVSSAATRPATEGQVTRGSRSGKRITGFAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3F8</accession>
    <name>U5W3F8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="9">Glycine--tRNA ligase beta subunit</fullName>
                <ecNumber evidence="9">6.1.1.14</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="9">Glycyl-tRNA synthetase beta subunit</fullName>
                <shortName evidence="9">GlyRS</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="8">Glycine--tRNA ligase alpha subunit</fullName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">Glycyl-tRNA synthetase alpha subunit</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="8" type="primary">glyQ</name>
        <name evidence="9" type="synonym">glyS</name>
        <name evidence="10" type="ORF">AFR_27410</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560081.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27410"/>
    <dbReference type="EnsemblBacteria" id="AGZ43743">
        <property type="protein sequence ID" value="AGZ43743"/>
        <property type="gene ID" value="AFR_27410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5556"/>
    <dbReference type="eggNOG" id="COG0751">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007220_0_1_11"/>
    <dbReference type="OrthoDB" id="213210at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00254">
        <property type="entry name" value="Gly_tRNA_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00255">
        <property type="entry name" value="Gly_tRNA_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015944">
        <property type="entry name" value="Gly-tRNA-synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002310">
        <property type="entry name" value="Gly-tRNA_ligase_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30075">
        <property type="entry name" value="PTHR30075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02091">
        <property type="entry name" value="tRNA-synt_2e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02092">
        <property type="entry name" value="tRNA_synt_2f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01044">
        <property type="entry name" value="TRNASYNTHGA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00388">
        <property type="entry name" value="glyQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00211">
        <property type="entry name" value="glyS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ43743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="978" mass="106002" checksum="B2F1A4C118F721FC" modified="2014-01-22" version="1">MLTMQDALARLTAYWTDQGCLVVQPMNTEVGAGTWNPATFLRILGPEPWRVVYVEPSVRPDDSRYGENPNRLQTHTQLQVILKPDPGNPQELYLGSLTALGIDVAAHDVRFVEDNWASPALGAWGLGWEVWLDGLEITQFTYFQQAGGLNLEPPSVEITYGIERIIMALQGVTHFKDIEYAKGVSYGEVFGQAEYEMSRYYLDDADVATNRRLLELYAAEAQRMIDEGLPVPAHSYVLKCSQAFNVLDSRGAVSTADRAAEFGRMRRLAGDVARLWVARRDELGHPLGVVEPLPAAAAFETVVEGTAPRTLVFEIGTEEMPPSEARTARTQVQRELTERLAGTRLAHGDIRVHATPRRLIAVVDAVAAREEDHVRTVKGPKTTAPEKAIAGFARSQGVSVESLQTAEVNGVAHLVVEKHEAGRAAPAVLGDVLAKVAGSLRSAKNMRWNDPELAFTRPVRWLVALWGDEIVPVAVSALLAGRETRVLRTAAQPRVSVASAESFMETLAINGIVADADDRRELIVTGAQDLVWDSGKIDVAGESALIDQITYLVEQPTPLLGTFDERYLELPSAVLTTVMRKHQRYLPVRDADGALLPMFVTVANGLVDVELVRAGNEAVLRARYEDAAFFYRADRQLPLAAMRDKLSRLTFTDKLGSMADRAGRIAALAGALADGPSPTLERAAQLVKFDLGSQLVTEMTSLAGVMARDYALHAGETREVAQAVYEAELPRQTGDDLPVSLPGALLSLADRLDLVTGLAATVGLPTGSSDPFALRRAVLGLLAVHRAHPALSGISLTDALAAAAALQPVEVPGRVLVDTGEFLAKRLEQVLVEEGHPVDRVRAVLPHAGRPSFADLLLAQLGRLIADPRFLALAEAIQRSRRIVPDGTAASYDPSVLKEPAEVALHEAVVSVRGELDAATDLTHFTAVAGRITDPINTFFDDVFVMADDPAVRAARLGLLATIRDLGADLLDWPQLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3M9</accession>
    <name>U5W3M9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bac_luciferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362947.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21525"/>
    <dbReference type="EnsemblBacteria" id="AGZ42575">
        <property type="protein sequence ID" value="AGZ42575"/>
        <property type="gene ID" value="AFR_21525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4365"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_6_4_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022480">
        <property type="entry name" value="F420_MSMEG2906"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03856">
        <property type="entry name" value="F420_MSMEG_2906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="2">
        <location>
            <begin position="19"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="285" mass="31506" checksum="51166D977E09A605" modified="2014-01-22" version="1">MTSSPRRLRIAVQLAPQHAGYTAIREAATAAEALGVDVIFNWDHFFPLGKIREGKHFEAWTMLGAWAESTSRAEIGALVSCVSYRNPDLLADMARTVDHVSDGRLVLGLGAGFREWEYTQYGYEFGTPAERVRDLGLALERIERRFAELNPPPTRPIPVLVAGGGPKTLRLVARHADIWHTFASGDELGEKSRLLDGFCHDRGRDPGEIERSTFVSGDPWEVAPAYREHGVTLFVVLASGPDFDLTDLKRWIAWRDEVTADARGGDRPAGTVPAQEASARTENRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W418</accession>
    <name>U5W418_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M16 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560216.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28090"/>
    <dbReference type="EnsemblBacteria" id="AGZ43879">
        <property type="protein sequence ID" value="AGZ43879"/>
        <property type="gene ID" value="AFR_28090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5694"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_547064_0_0_11"/>
    <dbReference type="OrthoDB" id="1790151at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="505"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="568" mass="60774" checksum="95D6FAA91D7236A4" modified="2014-01-22" version="1">MIRHTEVDGVPTLIAPTSGPMTAGLMFRVGRADETLPRTGITHLLEHLVLHPLGQADYHYNGATAPVTTYFHMQGPEKDVAGFLSGVCDALNDLPLHRLETEKEILRTEGSSRSSHVTEPMALWRYGARDHGLVSYPEWGLNMITPDDLLAWSARYFTRDNAVLWIAGRDVPPGLTLRLPGGERRPVPAPSSALPRTPAYFAGDSRAVALEAVVRRRTAASVFAGVLERELFRGLRQEGGLSYTAGTDYNPRGDGNAVITALADALPEKQDAVLGGFIDILAKIRVGRIEQTDIDAVVAKTVDALDNAEVYASRLPSHAFSLLTGDRQVPVDELAGELRKVTVADVHEVALEAFGSALLMVPEGRSADWAGFTEAPTTSDRAVSGTVYPHLENGAVRLMVGPEGVSVAGIQDVVTVRYDQCACVLGWPDGRRMLIGTDAVTVAVEPTMFGGAAAALPFIDAALPAQLRVVMPARDPQSIPQPDPQRHHAAVAGPAGATGSRSWSIAGIVVLVLMFLLFGCLSGSLALGLLVEDKPDNDLWVIVGGGTLLAGYLGYAVVRNIQRFRRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W442</accession>
    <name>U5W442_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Usp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363130.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22400"/>
    <dbReference type="EnsemblBacteria" id="AGZ42750">
        <property type="protein sequence ID" value="AGZ42750"/>
        <property type="gene ID" value="AFR_22400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4541"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881301_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="2"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="135" mass="14295" checksum="931A980B151B6B89" modified="2014-01-22" version="1">MRIVLAVKPGTDQPWITDAVARLARQTGASVAVVAVDHVELERFSPAPRTVFVQSARESADLAAQRLTAAGIDADATVRSGRPLEGTLAFAEEQQADLIVVGASTRPAVAERLLGSVPLELIKRSARPVLVITHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4I9</accession>
    <name>U5W4I9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363430.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23200"/>
    <dbReference type="EnsemblBacteria" id="AGZ42910">
        <property type="protein sequence ID" value="AGZ42910"/>
        <property type="gene ID" value="AFR_23200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4702"/>
    <dbReference type="eggNOG" id="ENOG502ZG2D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_971924_0_0_11"/>
    <dbReference type="OrthoDB" id="2109703at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="31205" checksum="BE9E8435AAEE2956" modified="2014-01-22" version="1">MSPNEPLPTPSEAGAILAQFRTSKQLFNDLVMKVEKEWENLCKQVDAMLARGVHRVNSDSAWDSFTGWIADVNPFQQALVELLEDIRTAFNKVRDIVNKLLPMLRKVVERSVPVLSLCEHAFIYLSNVNSPLGDLYTASDSLVPDAYYWGGPTKEHYVKEVVGDQQGALDRVTEHVQGLSTWLAQTASKNTDYMATIVHNISPIPNALSAAAANATAATVDFASAFFAIDNLAEALGNTVQAVLDQTADLMSYLVGVVAGIQELTVIKTERRDLAGGKWPQSVADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4V5</accession>
    <name>U5W4V5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560486.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44149">
        <property type="protein sequence ID" value="AGZ44149"/>
        <property type="gene ID" value="AFR_29440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5966"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_46_0_11"/>
    <dbReference type="OrthoDB" id="1309950at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041669">
        <property type="entry name" value="TetR_C_15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17918">
        <property type="entry name" value="TetR_C_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="23435" checksum="37BB71CD17A3C8AC" modified="2014-01-22" version="1">MPSDDRHLQPRRQPRQVRAELTRERILTAAAHIFAEYGYAAGTTNRIAERARISIGSLYQYFPNKAAILAKLLVRHIDRGTWTQADQLDLSPGSLRTTVQAMVRDAIDNHDDDPQLLRIMIEEAPVSKELLETINNHGRTRVTQIRDLLARHPDVKVQDLDTAADLITFTVEINTHRLMADPNAVAVETLEKELVDLVTRYLRGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W508</accession>
    <name>U5W508_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560640.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30215"/>
    <dbReference type="EnsemblBacteria" id="AGZ44303">
        <property type="protein sequence ID" value="AGZ44303"/>
        <property type="gene ID" value="AFR_30215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6118"/>
    <dbReference type="eggNOG" id="ENOG5033MZW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_948746_0_0_11"/>
    <dbReference type="OrthoDB" id="723872at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="32526" checksum="185576293D484B11" modified="2014-01-22" version="1">MTAVEAHFPGFADNMQKLLGHGLEFYLASNETLRPYLNEVPYGLKLSWLDFTDTTEDKGWDTGEFLNLFNVMNGIAFGDRGIPMPQWVMVDLILMPSAAVIAALPQSAFTEILETSAFDEDAKRHMRAVFDKAKQSDYNGPIPIAGYCAAPSAAPGTWVGWSLWSLWTSVGLGKALKALALSAYQAEKLDGVTQYDNSALRVHTRFGRLRVLVATVPFHTSPGSFVYENDFTVPVTATEPEPSFLLDPFDYERQRAMQKAIENRECTYYVLAPGHLVQDGTTYVPVLEIPTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5D3</accession>
    <name>U5W5D3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_24620</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363780.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24620"/>
    <dbReference type="EnsemblBacteria" id="AGZ43190">
        <property type="protein sequence ID" value="AGZ43190"/>
        <property type="gene ID" value="AFR_24620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4988"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_2_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="21"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="109"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="172" mass="19639" checksum="662478346811E56A" modified="2014-01-22" version="1">MSDELLVVRCQLGEREAFTELVQGWHPKVERYVGRMLGAPDDDVVQEIWLAVVKGLPRLRHPDRFAPWFFTVARRAVMNRLREAYTRPEPEPIDDVQGDDAAEGVVHRETLAVALAAIPAREREVLLLFYLEDLPLEACAQICAVPVGTVKSRLNRARRLLRQELVRKGYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5I8</accession>
    <name>U5W5I8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_30745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560744.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30745"/>
    <dbReference type="EnsemblBacteria" id="AGZ44409">
        <property type="protein sequence ID" value="AGZ44409"/>
        <property type="gene ID" value="AFR_30745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6224"/>
    <dbReference type="eggNOG" id="ENOG502ZM1D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200596_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="74" mass="7648" checksum="D975A18249FFA1B0" modified="2014-01-22" version="1">MLAIAAAIVFGLWLLLDLFDTNLGAPDLFNFSTMASLGLLLLSLYLAGVGSGPRGGSGSGSSGRRFYGRRPGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5K2</accession>
    <name>U5W5K2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363855.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24995"/>
    <dbReference type="EnsemblBacteria" id="AGZ43265">
        <property type="protein sequence ID" value="AGZ43265"/>
        <property type="gene ID" value="AFR_24995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5063"/>
    <dbReference type="eggNOG" id="COG4424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017703_1_0_11"/>
    <dbReference type="OrthoDB" id="1555988at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="PTHR10605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransfer_1" evidence="2">
        <location>
            <begin position="4"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="34968" checksum="5DA58F1254B89DD1" modified="2014-01-22" version="1">MPLPDFLIAGVPKAGTTALHAALVRHPGLFLPAVKEPKFFLSDGRPPVSGGPGDVQTYQEHVWRRADYEALFDPAPVGALRGEATPFYLYDLEAHDRIRTLVPDAKLILLLRDPVDRAHSNWTHLWNAGLEPEADFLTACRAEGQRRADGWADFWHYVSLGLYGRQVQHLYKLFPREQVLLLRYRDLKDTPAATLDRVCDFLGVPAGQLKEIPRENVNRHVVEDNAINRVLRGLLRTGGQFGHRFPVPLRLAARGPILTLLHRKHGTRPVTTPEERAALLPLFADDIALLQDVTGESYDDWMSVDRHAKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5R4</accession>
    <name>U5W5R4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560684.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30435"/>
    <dbReference type="EnsemblBacteria" id="AGZ44347">
        <property type="protein sequence ID" value="AGZ44347"/>
        <property type="gene ID" value="AFR_30435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6162"/>
    <dbReference type="eggNOG" id="COG1912">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080244_0_0_11"/>
    <dbReference type="OrthoDB" id="1345246at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002747">
        <property type="entry name" value="SAM_Chlor/Fluor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023228">
        <property type="entry name" value="SAM_OH_AdoTrfase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01887">
        <property type="entry name" value="SAM_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102522">
        <property type="entry name" value="SSF102522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="255" mass="26370" checksum="AC0EE05B6FFFA1D8" modified="2014-01-22" version="1">MLITVVADYGTGDLAFAEVRQTFAALVPQADVSPLPVPAFDTVSAGFCVAQLAFGAGPADRVIYANVAPRQDEDQPREDNAGERLAAARLPSGVLVVAVGSGASLSFLAAENIPLYAVKVADAGSQFRSRDVFPAAVAGLIAGDDSLLGEQLTVPPPPSRSVIYTDGYGNLKTSWYEPPAPTGTKVRVRIGDAEAEAVVSDGVFAVPSGALSFAPGSSGWPTTTGRRTCFELLSRGGNAAELFNFPRSGTPVELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5S2</accession>
    <name>U5W5S2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363871.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25075"/>
    <dbReference type="EnsemblBacteria" id="AGZ43281">
        <property type="protein sequence ID" value="AGZ43281"/>
        <property type="gene ID" value="AFR_25075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5081"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052492_1_1_11"/>
    <dbReference type="OrthoDB" id="1623593at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="34033" checksum="4B025F3C6F17A92E" modified="2014-01-22" version="1">MLIPAPPLTPYHRLARTAAHRWWRPLVGTVLVVVGGGALMLLLIGVLEIAGLVAGRPRNADGLSSFGPLTDLAGAFLMIAVFLPVTLLAARWVQRRPAGSLSSVTGRLRLRWLLTCLPVAAVAVVALLGGGITLAAATGEDVGLDTALAGWGPFVVATAVLLLVVPVQAAAEEYLTRGWLLQAVGTVCRSPWIPIAVQALVFAALHGWGTPWGFADLVVFGVVIGWLTVRTGGLEAAIALHVLNNLLSGLLGAAFGDLTVDETAADMPWQFVAVDVPVLLAYAALVLRLARRRGLDTRTPAERTTNLIESGQHAEPRMLAGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5T5</accession>
    <name>U5W5T5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560837.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31220"/>
    <dbReference type="EnsemblBacteria" id="AGZ44504">
        <property type="protein sequence ID" value="AGZ44504"/>
        <property type="gene ID" value="AFR_31220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6319"/>
    <dbReference type="eggNOG" id="ENOG50330B5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157972_0_0_11"/>
    <dbReference type="OrthoDB" id="2080374at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025329">
        <property type="entry name" value="DUF4235"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14019">
        <property type="entry name" value="DUF4235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="90" mass="9331" checksum="30C1F0FEBF83705F" modified="2014-01-22" version="1">MSSKLQKVAYKPVGLALGLGAGAIAGFVFKEIWKLASGDDDAPNATDEDRGWGEILAAAALQGAIFAAVRALVDRGGAVGVRRFTGEWPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5U1</accession>
    <name>U5W5U1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative alpha-L-arabinofuranosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_25200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 51 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25200"/>
    <dbReference type="EnsemblBacteria" id="AGZ43306">
        <property type="protein sequence ID" value="AGZ43306"/>
        <property type="gene ID" value="AFR_25200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5107"/>
    <dbReference type="eggNOG" id="COG3534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010060_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046556">
        <property type="term" value="F:alpha-L-arabinofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046373">
        <property type="term" value="P:L-arabinose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010720">
        <property type="entry name" value="Alpha-L-AF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06964">
        <property type="entry name" value="Alpha-L-AF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00813">
        <property type="entry name" value="Alpha-L-AF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666374" evidence="3">
        <location>
            <begin position="25"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-AF_C" evidence="4">
        <location>
            <begin position="475"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="835" mass="90364" checksum="B1AD3E43CF32A43F" modified="2014-01-22" version="1">MFGLTAVTVLLVGLGGAAPVFAEAAPDYTITVEPGARGPEINEAMYGVFFEDINFGADGGLYPELVRNRSFEFLPADNAAYTGLTAWTPSSVGGGAGEASTVNDDARLNERNRTYLKLDLDNPAGGRFGVTNDGWNTGFAVERGKTYDFSVWARSDAAGGTPISVVAADAGGEAWAKPSALTARGDRWTRYSVPVKATRTTDRGRLAVLAGGTGTLRLDMVSLLPRDTYRGHGLRRDLAEKVAALEPGFVRFPGGCLVNTGSHEAYDAASDFPRARSYQWKDTVGPVETRATNTNFWGYNQSYGLGYYEYFQFSEDLGAMPLPVVPALVTGCGQNRATDDPELLQRHIQDTLDLIEFANGPVTSTWGKLRRDMGHPRPFGLTHVAVGNEENLPDAYFANFLKFREAIAAKYPGITVVSNSGPDDQGATFDSLWAKNRAAGVAMVDEHYYNSPAWFLGNNKRYDTYDRSGPKVFLGEYASLDNKLVNSLAEASYMTGLERNADVVRMASYAPLFANVDHVQWRPDLIWFDNDESWGSTSYQVQKLFMTNVGDRVVPSRATGRVIQPKPITGAVGLSTWATAAAYDDVRVTSPGGAVLFSDDFSDGAGQWTPVEPRGAWSVVDGAYRQIDTAALDTLVKAGNITATDYDLTLKATKQSGAEGFLVAFGVQDSGELYWWNLGGWNNTQHAIQKGAGGSKEIVLAKPGSITAGQTYNIKIEVRGTTVRLFLDGAEWGSFTDDQVTEPFAQVITHDDRTGELIVKVVNAQDTPAVTTVDLAGRKVASRGRMTVITGDPQEQNTRTAEPIQPVTTTVTGLGSTFTRTFAPNSVTFLRLETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W603</accession>
    <name>U5W603_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364028.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25585"/>
    <dbReference type="EnsemblBacteria" id="AGZ43381">
        <property type="protein sequence ID" value="AGZ43381"/>
        <property type="gene ID" value="AFR_25585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5185"/>
    <dbReference type="HOGENOM" id="CLU_149127_0_0_11"/>
    <dbReference type="OrthoDB" id="2069053at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="15428" checksum="E2AC1510EC36A268" modified="2014-01-22" version="1">MSLLDVGKLALITVTPTLIGALVVYAPKWCSAVARAWPRTTADEPVPAGPPIEQLAADLRRLLRLHDELTGSAHIAMRAHRLWAVEAAISVRAVEAAQALDVPHLEPAPDGMLSRTELGRLLHALADSGLVLPATVGPFTKDGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6N6</accession>
    <name>U5W6N6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561210.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33075"/>
    <dbReference type="EnsemblBacteria" id="AGZ44873">
        <property type="protein sequence ID" value="AGZ44873"/>
        <property type="gene ID" value="AFR_33075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6694"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062375_0_0_11"/>
    <dbReference type="OrthoDB" id="1456605at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="328" mass="33958" checksum="597FD852FE82C8F1" modified="2014-01-22" version="1">MTWVVADTIWWDGRVHRESALCLTPGARKVVPVAEVPAGEPRRRVPGAVLPGLCDAHVHSALIDLPTLRAGGIAYVWDLGGVPAAVAAWAGRFPMRYAGPFLIAPGGYPSDRSWAPAGSCREVLSAGDAAAAVAEVVAVGATLVKVTAHAGGPLLPPATLEALVSAAHTAGLPVVVHAEGPGTVVAAYRAGADLLAHTPWTSALDAGLVRDAAARMTWISTLDIHGRDTPTPAWETALGNLRLFLEHGGKVRYGTDLGNGPLPPGINVRELRALQSAGLDPASVLATMTEADHAEPTWLPAGLDLDPETFAGSLATARVLTPEVRPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W719</accession>
    <name>U5W719_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561135.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32695"/>
    <dbReference type="EnsemblBacteria" id="AGZ44797">
        <property type="protein sequence ID" value="AGZ44797"/>
        <property type="gene ID" value="AFR_32695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6616"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076820_0_0_11"/>
    <dbReference type="OrthoDB" id="1824113at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="252" mass="26232" checksum="547E60FF57E57804" modified="2014-01-22" version="1">MALLPSPGAFVELSRYAIGQVVETAASVATVPVRVLGLLGQTELLVSRISLLAERAEGLIGRVEGVIDGVDTTLRETKVIMAAAALTVDEASAVALRAAQVVDGAASAVTGASAIVTEAGTVVTKAGKTADDAGGVVVQAKATADEAGALLDGYAPTLRSAAPLAQRFVEELTPEEVTAAIRMVDELPRLRQHLSEDVMPLLGKLDQVGPDLHKLLEVTNDLHLAIIGLPGLKRLRRRGEERAAEEEAANDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7V0</accession>
    <name>U5W7V0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561550.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34790"/>
    <dbReference type="EnsemblBacteria" id="AGZ45214">
        <property type="protein sequence ID" value="AGZ45214"/>
        <property type="gene ID" value="AFR_34790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7039"/>
    <dbReference type="eggNOG" id="COG1529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_2_11"/>
    <dbReference type="OrthoDB" id="117507at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="PTHR11908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="Ald_Xan_dh_C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="SSF54665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="SSF56003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ald_Xan_dh_C" evidence="1">
        <location>
            <begin position="23"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="688" mass="73463" checksum="06D0066FE28F0301" modified="2014-01-22" version="1">MTTTFSSAVGQPLARIEGPAKVTGAALYAAEYPVDGLAYAWVVQSPIARGTLNAVVVDPTTEDNDVIAVLWHGNAPRLEPADDPELDVLQSEHISYRGQAIALVVARTLEAARRAAQVLDLDLTEEPHDSLLTADHPELYTPEKVNPSFPAVTDTGEFEAAYAAAPVTVDVTYETPALHNNPMEPHATTAVWEGDSLVLYDSNQHPPGVAAAVGKVFGLSADRVHVITEHVGGGFGSKGTARPNAVLAALAAKVVGRPVKLALPRQAMFSMVGHRTPTIQRLRLGAQTDGTLTAIGHDAVEQTSRLFEFAEQTTVATRHMYAAANRHTGHRLVRLDMPTPRWMRAPGEAPGMAGLECALDELAEKLGQDPVEFRIRNEPAAEPETGKPFTSRNYVACLREGATRFGWADRDPRPRARREGQWWIGTGVAGATYPTMAQPSTARARRLGDGRFEVAIAATDLGTGARTILTQIAADALGAPAEAVTLRIGDSTLPMASVAGGSSGSASWGWAVTGACTELRGSGRDEAFFDSTELVQQQEKDGKHAYGAHFAEVRVDADTGEVRVSRLLGVYAVGKVLNPRTARSQFIGGMTMGMGMALHEEGVLDPAMGDWVNHDLAEYHVPVHADIESIEATWIEEEDTTVNPMGSKGIGEIGIVGSPAAIVNAVWHATGVRVRDLPVRLDKLLPHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7W1</accession>
    <name>U5W7W1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34865"/>
    <dbReference type="EnsemblBacteria" id="AGZ45229">
        <property type="protein sequence ID" value="AGZ45229"/>
        <property type="gene ID" value="AFR_34865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7054"/>
    <dbReference type="eggNOG" id="COG0122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052970_0_0_11"/>
    <dbReference type="OrthoDB" id="1781735at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32932" checksum="2F18756AB7A81574" modified="2014-01-22" version="1">MTRVTRTRRLSLPDGYHFRATLRSLLVVRYDPCGTLTDTDFWLSARTPEGPGSLHLARAGAELTATGHGPGADWLVDRADAFAGLRDDVSDFPALAAAHPLVARLARTFPGVRYPASGQVFHRLIRAILEQKVTGIEAHRAYRSMMKHFGEPAPGPAELLLPPDPEAVAATPYWTFHPFGVEQRRTQALLRAALVAARLERCVDSAEATRRLTAVPGIGPWTAAEVVRTAFGDADAVSVGDFHIPNTVAWGLAGEARGTDARMLELLEPFRGHRGRVCDLLAMGGISAPKFGPRMPIRSFAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8N5</accession>
    <name>U5W8N5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative rRNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560687.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30450"/>
    <dbReference type="EnsemblBacteria" id="AGZ44350">
        <property type="protein sequence ID" value="AGZ44350"/>
        <property type="gene ID" value="AFR_30450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6165"/>
    <dbReference type="eggNOG" id="COG0566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021322_3_1_11"/>
    <dbReference type="OrthoDB" id="1697536at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="1">
        <location>
            <begin position="29"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="26821" checksum="384E7D968695E4A6" modified="2014-01-22" version="1">MTFSARTPRVVAARRLQRRRDRDQTGRFLAEGPQAVRSALAAGVVLELFGTDTALSRHADLAAQAPLVSPVTDDGLEALAETVQPQGLVAVCEQVDVPLTEALSKAPRLVAVAVEIRDPGNAGTVLRTADAAGAGAVIFAGDAVDPYNGKCVRASAGSLFNVDVVQAPLQVVDQLRDFGLQVLATTGKGDAGLDELLDDGALAQPTAWLFGSEAHGLPEHLLKAADKRVRIPIYGGAESLNLAAAAAVCLYASARAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W971</accession>
    <name>U5W971_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_37150</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562020.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37150"/>
    <dbReference type="EnsemblBacteria" id="AGZ45684">
        <property type="protein sequence ID" value="AGZ45684"/>
        <property type="gene ID" value="AFR_37150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7516"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046120_0_0_11"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="28"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="31700" checksum="C36B4874779ABE8C" modified="2014-01-22" version="1">MSDPRTTPSIFTRGAVDLGALRPANKPAPAGPAGPATSAAPAPSPGGAPPAVIEVTEENFQTTVLELSMTTPVILDFWAEWCEPCKQLSPVLEKLAAEGAGSWVLGKVDVDTNPRLAQALRVQGIPMVIAVVGGQLVEGFTGVLPEAQVRQYVDAVLKAGGVEVAAPEDPRLDAADDAMMTGDLDAAEAAYKKILAESPQDHAAESGLAQVELYRRITGVDAEKALAKAAADPDDLEAQQLAADIEVLSGQAPEAYDRLVSAVKRSSGEERDAVRKHLLSLFNVAGPDDPAVASARRALARALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9J0</accession>
    <name>U5W9J0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_31555</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31555"/>
    <dbReference type="EnsemblBacteria" id="AGZ44571">
        <property type="protein sequence ID" value="AGZ44571"/>
        <property type="gene ID" value="AFR_31555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6387"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="361"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="501"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="769" mass="81839" checksum="459E9D18203825D9" modified="2014-01-22" version="1">MTLVLVAAVTAIGAWVVPDGPAQRLYDDLAQLAAGVLAAVICFRSGLKRSGARRRWRLLIAAGLTTWAAARLWWVVRDLVDPDRSAAAVPADVGFLTLPVFTFLALIAGSMALPRPAQASLRRDRVVLVIDAFLITGSLLALIWSALPGRIFDEVGDNPLVVGAAAVYPIADLMLVVVGVLLLTTRPTSAAGRVPLRLMTLAMLAFGVSDTLRMLHLSTGVLTPWESAGYLLGPGLLALAALTNPTLTDQPVVAPAEADWLHLLLPYVPVVATGVVIAVRTGAGGQLTSYEAYLGWLGLGLVVARQMFTIVDNTVLLARVSEGQQRLHHQAYHDPLTGLANRALFRERLVLALDGHRHRRMPVALLFADLDDFKLINDSFGHAMGDRLLRAIGDRLIASVGPGDLVARLGGDEFAVLLERPASEAEPAAQRILTALREPFEIDGHTVGISASLGLVLPEPGDVDLSADTLLRRADAAMYVSKRRGKNALARYTGGSDGGPNADLPHLLARALSGSPAANGFEVHYQPIVRFSDGAIVAVEALARWTDPIAGPVDPDVFVTVAERTGLVAAIDDFVLDRACADASALANTYGRPIDMHVNVSAARLGQHGLEEAVDRALQRHALRPSRLVVEITETRRIPDLPRAIEVAERLRRRGVRVALDDFGSGYNALAQLHSLPVDIVKLDSTLTDVDVSPERAGALCRSVLAICAELNIAVVAEGIETPARARALAALGCDLGQGYLFGQPGPVHRLTATIPTQPTPHSSPHLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9M5</accession>
    <name>U5W9M5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coagulation factor 5/8 type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_32085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32085"/>
    <dbReference type="EnsemblBacteria" id="AGZ44675">
        <property type="protein sequence ID" value="AGZ44675"/>
        <property type="gene ID" value="AFR_32085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6496"/>
    <dbReference type="eggNOG" id="COG3250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014141_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666201" evidence="3">
        <location>
            <begin position="24"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="4">
        <location>
            <begin position="9"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="4">
        <location>
            <begin position="156"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="4">
        <location>
            <begin position="997"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1132" mass="119699" checksum="B86CCD8D37138C40" modified="2014-01-22" version="1">MIPVTVLAVVAAYLGVVQLTAHAADSLLSQGRTATASSQEGADVAAANAVDGNAGTRWSSVFADPQWLQVDLGATATINQVVLQWEGAYGKAYKIQTSANGTTWTDVYSTTIGAGGTETLAVTGSGRYVRMYGTARASGYGYSLYEFKVYGSTGATPPPAECGTSNAAQGRTATASSQEGADVSAAKAVDGDAGSRWSSVFADPQWLQVDLGSSQTICQVVLQWEGAYGKAYKIQTSANGTTWTDVYSTTTGAGGTETLTVSGTGRYVRMSGTVRGSGYGYSLYEFKVFTGGGGTPPTDPTTPPPIPGNFTTVWTDDFTGGANTSPNSANWLLRTGTQYPGGAANWGTGEVETASDSTANVYLDGAGKLNIKAIRDGAGKWTSGRIETQRTDFEPLAGQQTKFTAVLKQPDVANAAGYWPGFRATGAAYRGNYTNWPGVGETDIMTDVNGRSQLSQTLHCGTAPDGPCAEYNGRQSGLASCTGCQTGFHEYTQIVDRTRTDEEIRFYLDGRQTWVVRQSQVGVTAWNAAVHHGFFLRFDLAIGGSLPNAIAGFTTPTPETTSGGVLSVDSVTVARATGTTPAAMTDPATPSGPSTVRVTGSQGNWQLTVNGSPYEVKGITYGPPQAAADGYMRDLKNMGVNTIRLWGVDDTNTPVLLDRAAQQGIKVVVGHWLNQGADYVNDTAYKTNTKNEIVARVNALKGKQGVLMWDVGNEVILTMQDHGLSAAEVEARRVAYAKYVNELAVAIHAADPNHPVTSTDAYTGAWKYYKADSPALDLLAVNSYGAIGGVKQDWISGGYTKPYILTEGGPDGEWEVPNDVNGVPTEPTDLQKRAGYTASWNAIKSHPGVALGATEFHYGLENDFGGVWLNTFTGGWRRLGYHALKQAYTGQASANTPPEINSMSVSNQTAVPAGGTFTINAGVSDPNGDLIRYNLMYSNKHITGGTGLTNVVFTDNGNGSFTAKAPEQLGVWKVYVYAFDGQGNVGIDQRSFKVVPPTIPGTNLSQGKTVTASSYQPTGTNGPQLPAYAVDGNYSTRWASEWVDTAWVQVDLGSVQSFNRVLLAWEAAYAKGYTVQVSDNGSTWTTVHTATAGNGGFDDLAISGTGRYVRVNGTARATDYGYSLWELGVYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9N4</accession>
    <name>U5W9N4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetate kinase</fullName>
            <ecNumber evidence="6">2.7.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ackA</name>
        <name evidence="8" type="ORF">AFR_38000</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>acetate + ATP = acetyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:11352"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Mg(2+). Can also accept Mn(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the acetokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.1" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562189.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38000"/>
    <dbReference type="EnsemblBacteria" id="AGZ45854">
        <property type="protein sequence ID" value="AGZ45854"/>
        <property type="gene ID" value="AFR_38000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7685"/>
    <dbReference type="eggNOG" id="COG0282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020352_0_1_11"/>
    <dbReference type="OrthoDB" id="537106at2"/>
    <dbReference type="UniPathway" id="UPA00340">
        <property type="reaction ID" value="UER00458"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008776">
        <property type="term" value="F:acetate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00020">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004372">
        <property type="entry name" value="Ac/propionate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000722">
        <property type="entry name" value="Acetate_prop_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00016">
        <property type="entry name" value="ackA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01075">
        <property type="entry name" value="ACETATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="181"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="255"/>
            <end position="257"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="303"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="366" mass="38597" checksum="F41168597BF13270" modified="2014-01-22" version="1">MKILVLNTGSSSVKYRLFDGVETLAKGLIERIGEQGGEVPDHAAALQRVMSSVDLEGLGAVGHRVVHGGAEFAGPTVITDEVVATIERLVPLAPLHNPAALTGISVARKLLPDVPQVAVFDTAFHQTIPPEGVAYAIDADLADRWQIRRYGFHGTSHAYVARQTAELLGLSPDDAKVITLHLGNGASACAVDGGRSVATSMGMSPLAGLVMGTRSGDLDPTVIFHLHRVAGLPVDEIERSLTRYGGLQGLTGDNDMRTVQRRRDEGDPAATLAFDLYCRRIKEYVGSYLAVLGGADAITFTAGVGENAPAVRAASLAGLSALGVTIDEARNQRGDRIISPENARVAVCVVPTDEELEIAQQVTAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9P0</accession>
    <name>U5W9P0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_37415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562072.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37415"/>
    <dbReference type="EnsemblBacteria" id="AGZ45737">
        <property type="protein sequence ID" value="AGZ45737"/>
        <property type="gene ID" value="AFR_37415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7568"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050005_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="449" mass="48794" checksum="920E4644C6A46796" modified="2014-01-22" version="1">MTVIELGLVTDGGDQPLSDHERRPLGRRDVRRALVAVVALFSVLTVTGSARPDPKGLPQLWSIPYTEGADTFMLDAGNVYVLSQPDGSRITAYDSRTGARRWSTQGVTDTTWMGWARSGVLLMPAGFTTTRYQDVDGSEVSREFSRETVALDTATGRVLWRRTGEFSVAVSGRALLTEWNAEGSKARTFQVVRVSDGATVWSRPAAGLETWATDWMNHDSSDRLVAAAPDGRIEVFDLSDGRLVAGGTVPWVRQSTADNEFTSLSIDGHRLYIDRSMRERTAVTAYDTETLGRLWEVERPSSGGVYGCGPVLCVNGPDDTAGYDRDTGEVRWRLAGAANGFPMPGGRVLLDDDETGARHHLVDGATGKRLADLGAAVPVWNATATDLPYLLRRTKEPPGLMSISRIDENTGEELLRGTLPQVLDYGCQSEDDLLVCATPDSRLVVTDVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Z1</accession>
    <name>U5W9Z1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_37915</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562172.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37915"/>
    <dbReference type="EnsemblBacteria" id="AGZ45837">
        <property type="protein sequence ID" value="AGZ45837"/>
        <property type="gene ID" value="AFR_37915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7668"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_11"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="42913" checksum="EDF6BC6D1702C1D6" modified="2014-01-22" version="1">MVAIAARGLTKSFDDDGVAVDHVDLDVAPGELLVVLGPTGCGKSTLLRLLAGIEEPTSGAVLFDGVPVTEIDPRDRNIAMVFQDYALYPHLTVAQNIGFPLRTVPVPAADIGVRVAEVARNVGIADLLNRYPEHLSGGQRQRVAMARALIRHPSVFLLDEPLSNVDAGARSALRGEIVALVRRLGVTTVHVTHDQIEAMSMADRVAVLRAGVLQQVGPPAEVYADPDTLFVAAFLGTPRTSLLEAAVYAEDNRVVLDFGSQVLELPADDPRTAVLAEHHTDRVTVAMRALTPGVDGMVLRGKVTTVDNLGHELLAHVDVGGVPSPWLDTPPGLRAAARAESTPYGFYPAYDVVEPLPMGEVMVRVAMPGRVLPGDEMAVGVTLDDLLLFDRRGLRIRFIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA75</accession>
    <name>U5WA75_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39120"/>
    <dbReference type="EnsemblBacteria" id="AGZ46078">
        <property type="protein sequence ID" value="AGZ46078"/>
        <property type="gene ID" value="AFR_39120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7917"/>
    <dbReference type="eggNOG" id="ENOG502ZYGE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_665298_0_0_11"/>
    <dbReference type="OrthoDB" id="1095437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="373" mass="41758" checksum="6C53BDA108790E49" modified="2014-01-22" version="1">MVERGMTAALVAAHRYSHGRLLAHPEAAVAGDVPAIIVPTVRNPATMRDSLELARELQRPMIALCSQWSSAEIVRKQAELLGAPVVAVDVTAKNGLPAFECDTLLAKQKHLNRANDVSIKRNLGLAVARMQRWEKVAFLDDDITGLRPEAIRNAGGLLRDYNVSALRNDGFPDNSVVCHARREVGLPQDVFVGGGAMAVRVNSRTPFFPTIYNEDWLFLVGRRNVERVAVYGRVSQQPYDPYLSPERARSEEFGDCLAEGLFSLSDHDRPMQAADERFWRAFLDHRIAMIDDILRRLDGQRHGERRTRIIQALKVARGRCQIIEPAFCVAYLKAWQTDLDVWKRFQAALPRHDNPVAAFTALGLKAYAYTPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAA4</accession>
    <name>U5WAA4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0225 protein AFR_33090</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0225 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561213.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33090"/>
    <dbReference type="EnsemblBacteria" id="AGZ44876">
        <property type="protein sequence ID" value="AGZ44876"/>
        <property type="gene ID" value="AFR_33090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6697"/>
    <dbReference type="eggNOG" id="COG3012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099590_2_0_11"/>
    <dbReference type="OrthoDB" id="1472440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00612">
        <property type="entry name" value="UPF0225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023006">
        <property type="entry name" value="UPF0225"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="133" mass="14755" checksum="C878433D1FF39D7E" modified="2014-01-22" version="1">MARRSPRLTTCPCGLGQPYAECCGPAHEGKPAATAEALMRSRYAAFALDDAAYVLRSWHPDTRPADVEPDPQMRWTGLDVLESTGGGLFDAEGVVEFTAHYRDGGRPGEMRERSRFVRHDGQWVYWGPVLTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAD5</accession>
    <name>U5WAD5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562321.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38665"/>
    <dbReference type="EnsemblBacteria" id="AGZ45987">
        <property type="protein sequence ID" value="AGZ45987"/>
        <property type="gene ID" value="AFR_38665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7823"/>
    <dbReference type="eggNOG" id="COG1807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_536154_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="558" mass="59716" checksum="C82A5CA5D3EA2EB2" modified="2014-01-22" version="1">MLTAERTEHRTSVAERLDRRPIVIYAGGVALAVAVYFVNLSAGLDFDLDEVMYALAGQNVATDGSVSWGSQPTTVHPPLHFLLIGAWTLLSGSAHGDLVGAILHARQLGAFFSVLTVLMVGLLARRYAVGRGAAARTWIVAGAMVLATFDGFLVRFGRTGLIEPTAIFVGLLVVYLSLRLRNATSPVYLGVVGVVSGLALLVKEPLIFTIVVPALAGLLERDWAYLRRSLAALLIGLLVWGTFPLWAVVNGAGGWWWSEHDTSLNRLAGFLQVSGLNRSGVSSAGVFGDTFTMYLSGYLIFSIGAAGLLIAAYRGGLFHRRRLGRQAAGLVAFGVVSYGFLAYCVLLGQANEQLTVYSAAPAVLLSVLAWGRPTPRAAIVACALAALVGVTTWTTDVVLTRDDATIRMGRYLATHNACSPVNATGNAMRWAPALTANHVYAFTDGPTALNAGLHLFLLSPKDSRLRYGNSSPELDTWVRTRGRPVLQLPSRRYERIELWSVPGPSVVTGEQCGNDRPPVSAQASSTRFLVLLAALIICLAAALYAWSIVRDRPDGDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAJ3</accession>
    <name>U5WAJ3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Ribonuclease H</fullName>
            <shortName evidence="5">RNase H</shortName>
            <ecNumber evidence="4 5">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnhA</name>
        <name evidence="7" type="ORF">AFR_33540</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="4 5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33540"/>
    <dbReference type="EnsemblBacteria" id="AGZ44966">
        <property type="protein sequence ID" value="AGZ44966"/>
        <property type="gene ID" value="AFR_33540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6788"/>
    <dbReference type="eggNOG" id="COG0328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030894_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09278">
        <property type="entry name" value="RNase_HI_prokaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00042">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022892">
        <property type="entry name" value="RNaseHI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="6"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="146" mass="16172" checksum="7778B236386DE887" modified="2014-01-22" version="1">MEIYTDGACIPNPGPGGWGAVLRWGTTEKDLCGGEAGSTTNNRMELMAPIQALESLTRAPLLVMLYTDSIYVRDGITKWLPRWKANGWQTSAKQPVKNVDLWQRLEAAVTRHDVQWHWVKGHAGHPENERADRLAARGLQEAIAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAU1</accession>
    <name>U5WAU1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">T-cell immunomodulatory-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34135</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34135"/>
    <dbReference type="EnsemblBacteria" id="AGZ45085">
        <property type="protein sequence ID" value="AGZ45085"/>
        <property type="gene ID" value="AFR_34135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6907"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_564571_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13517">
        <property type="entry name" value="FG-GAP_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666249" evidence="3">
        <location>
            <begin position="21"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="512" mass="54683" checksum="B1B36B1C474D0191" modified="2014-01-22" version="1">MLVAVTLAAGSGFLAAPARAEGPLVPAAPALQNAPIRHAPCVAGGPTRADKKTADRVRSSLNGRRLGRSVSAQSISCARAIVRNVQASGLGWRAAVIAVTTAITESTLHNYVVAVDHDSLGLFQQRPSSGWGRPGQLVDPGYATRAFLNAMIRKHPSAGWTTGDIGQICQRVQGSAYPEAYAPEVHDAQLIVAELWTRAAAPDPTGTAAAPAVVDKPKTPKKPKGPFQNPLVTTVTGMGSTDARSDMVTADWNANGTTDLAVVQRNGTGSGQTELFIFEGAPTFQRHFQRLLLHTATVLGPTDERHSFSLADWNGDGRLDLFVVQKSGTASGRTELRIVDGASSFRSYLLEVSTGFGPGDDRHAFSVADWNADGKQDLVMIQKSGNTNGRTDIRVLDGASNFQRYLVDTPTALGPTDDRHEVSVTDWNADRRLDLVVVQKSGTASRKTEVRVLDGASTFRRTLVKATTAQGPADDRHELLVTDWNRDGRPDLMVVQKADTVSGWTEARVLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB68</accession>
    <name>U5WB68_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-asparaginase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562721.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40675"/>
    <dbReference type="EnsemblBacteria" id="AGZ46389">
        <property type="protein sequence ID" value="AGZ46389"/>
        <property type="gene ID" value="AFR_40675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8232"/>
    <dbReference type="eggNOG" id="COG4448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062004_0_0_11"/>
    <dbReference type="OrthoDB" id="1434447at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010349">
        <property type="entry name" value="Asparaginase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42110">
        <property type="entry name" value="PTHR42110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06089">
        <property type="entry name" value="Asparaginase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="32324" checksum="47BC611900BE5D6C" modified="2014-01-22" version="1">MILAEVVRSGFAESVHHGSVVVLDTAGQVVAEAGDVTGPTFPRSSNKPLQTVGMLRAGLRTAEPADLALISGSHFGEPFHVARVRQILETAGLDESALRCPASLPLSEDERDIVLRDGRGAERILMNCSGKHAGMLATCVANGWPLDDYRDVKHPLQVTLAATVSELVGEPIAATGIDGCGAPVLGFSLTALARGFQRLVQAPPQSFERLVADTMRADPQLVAGTGEDDTRLMQGVPGLLAKGGAEGVVAVAVEGAGAVALKIEDGAMRARMPVLVSALRRLGVDAPVLEELATVPILGGGVRVGEVRTAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB75</accession>
    <name>U5WB75_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561528.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34675"/>
    <dbReference type="EnsemblBacteria" id="AGZ45191">
        <property type="protein sequence ID" value="AGZ45191"/>
        <property type="gene ID" value="AFR_34675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7016"/>
    <dbReference type="eggNOG" id="COG2358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033215_4_1_11"/>
    <dbReference type="OrthoDB" id="991310at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011852">
        <property type="entry name" value="TRAP_TAXI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42941">
        <property type="entry name" value="PTHR42941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16868">
        <property type="entry name" value="NMT1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02122">
        <property type="entry name" value="TRAP_TAXI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666483" evidence="1">
        <location>
            <begin position="30"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="334" mass="34606" checksum="2293FD0A36EDFAA5" modified="2014-01-22" version="1">MRKAFALAAAGTLTATALVGCGGKQDAAAEDAGGTVTCEVANDTRIGIATGNATGVYFALGNAYAEQIGASGGKVKATASETGASVQNIQQLVAGEFDVAFSLADSAADAVNGTNSFTTKQPVQALARIHTNYTQVITRKGSGIKDVAGMKGKRVSTGSPRSGTEVIAKRVLTAAGLDPDKDVQAQRLDLTKTVDGVKDGSIDALFWSGGLPTPGITDLLTTSADQVEFLDITPLLPELQKINPVYETGTIPATTYKTPADIPTIVVPNLLLVRENLDANVACVLTRTLFDRKPQLEQANAAAKEITLDKARKTTPVTLHRGATTALDALNAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB77</accession>
    <name>U5WB77_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferric uptake regulator family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562575.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39940"/>
    <dbReference type="EnsemblBacteria" id="AGZ46242">
        <property type="protein sequence ID" value="AGZ46242"/>
        <property type="gene ID" value="AFR_39940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8081"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_4_0_11"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="15732" checksum="68F006339973D2CA" modified="2014-01-22" version="1">MPTGEQLLRANGLRVTRPRMAVLDVLTQGGHLEVEEITRQVRERLDSVSTQAVYDVLGALSRAGLARRIEPAGSPARFESRVGDNHHHIVCRSCGTIEDVDCAVAERPCLDPGTGHGFEVDEAEVTFWGLCPSCQRRRGADRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCI5</accession>
    <name>U5WCI5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="8">KARI</shortName>
            <ecNumber evidence="8">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="8">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvC</name>
        <name evidence="12" type="ORF">AFR_36885</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561967.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36885"/>
    <dbReference type="EnsemblBacteria" id="AGZ45631">
        <property type="protein sequence ID" value="AGZ45631"/>
        <property type="gene ID" value="AFR_36885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7462"/>
    <dbReference type="eggNOG" id="COG0059">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033821_0_1_11"/>
    <dbReference type="OrthoDB" id="188901at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="PTHR21371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="IlvN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8 9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="8 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="10">
        <location>
            <begin position="3"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="11">
        <location>
            <begin position="184"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="26"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ45631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="337" mass="36235" checksum="07AA0200B7F8D36E" modified="2014-01-22" version="1">MTAEVFYDDDADLSIIQGRKVAVIGYGSQGHAHSLSLRDSGVDVVVGLKEGSKSRAKAEEQGLKVLTPAEASAWADVIMVLAPDTAQRKIYTESIEPNLTAGKALFFGHGLNIRFELIKPPANVDVAMVAPKGPGHLVRRQYVDGKGVPALIAVEQDPSGGAQALALSYAKAIGGTRAGVIKTTFKEETETDLFGEQAVLCGGTAALVQTGFEVLTEAGYAPEIAYFECLHELKLIVDLMYEGGISRMRYSVSDTAEFGDYVSGPRVINASVKQEMKKILAEIQSGEFTKALIADDEAGGTRLQQFRKEGAEHPIEVTGKKLRDMMSWVDRPITETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCT7</accession>
    <name>U5WCT7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">GTP 3',8-cyclase</fullName>
            <ecNumber evidence="3 12">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">moaA</name>
        <name evidence="14" type="ORF">AFR_42665</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer and homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="3 12"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42665"/>
    <dbReference type="EnsemblBacteria" id="AGZ46787">
        <property type="protein sequence ID" value="AGZ46787"/>
        <property type="gene ID" value="AFR_42665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8634"/>
    <dbReference type="eggNOG" id="COG2896">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_11"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019008">
        <property type="term" value="C:molybdopterin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_B">
        <property type="entry name" value="MoaA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="Mob_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 12" id="KW-0408">Iron</keyword>
    <keyword evidence="8 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="5"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="265"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ46787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="333" mass="36120" checksum="9A63642795FC2518" modified="2014-01-22" version="1">MLADRFGRVATDLRVSLTDRCNLRCTYCMPEEGLAWMPKEQQLSDDEVGRLIRIAVEQLGVTEVRFTGGEPLIRRGLVGIIAAAAALNPRPRLSVTTNGIGLERMAGPLLEAGLDRVNVSLDTLLPERFLALTHRDRHADVLKGLAGAARAGLTPVKINTVLMRGVNDDEAPALLQFALDHGYELRFIEQMPLDAQHQWDRHEMVTAEAILASLEPFGLMPDPVSRGTAPAETWLVPGYRDAAGNPARVGVIGSVTRPFCGDCDRTRLTADGQIRNCLFATDESDLRGLLRADAADADIAAAWQTAMMGKRAGHGIDDPLFLQPTRPMSAIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD10</accession>
    <name>U5WD10_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translation initiation factor IF-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38255"/>
    <dbReference type="EnsemblBacteria" id="AGZ45905">
        <property type="protein sequence ID" value="AGZ45905"/>
        <property type="gene ID" value="AFR_38255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7739"/>
    <dbReference type="eggNOG" id="ENOG5030HTA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_513548_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="530" mass="54685" checksum="F3DC5A8E8396CF3E" modified="2014-01-22" version="1">MVMLDQHFLSPSATPSPFGPLQGGERELWRGRTAVAEYAFDEAASLPRWALPETTEVLVTNHRVVYAYTASGLPDDVEVTSGELRWLWPQHLRVQPGARSQGRAASAAQIQLVCGSADGSFPALVFAGGDLTTVADADRLANLIRHAIAGFRVDNADKLGLSTSQARMLSRLLIGPEFSNYQGGEGQTVSVLGALPVTRPAPDTTPALEPAPVLPATPALDATLVLETPPVLETAPVFEPASTPALTSATVDDFTDDDLAASDAFTSSDAFPSSDAFTSSDAFPSANAFAESDAFAAEHGFDTGRGYDTGRGYGTELGYGTGLGYGTGRGFGTERSVAAGHAVDAEPGYVDPQQYDEFHPADVSSATLRLPGYRPGLAADHARALQAAAAEEATQISQPGLASRAADLAARVASLVQSTDAQADPAPPAYLIPAAPPVTSALSADTVNLIPLPAANSPTSAGPVPTASSAGSAATDSADSDDVPTADLTARADNVRRAAARFAANSARGKAGPRRPEHEIGSGTRGNRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDY6</accession>
    <name>U5WDY6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Biotin carboxylase</fullName>
            <ecNumber evidence="2">6.3.4.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_39385</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562465.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39385"/>
    <dbReference type="EnsemblBacteria" id="AGZ46131">
        <property type="protein sequence ID" value="AGZ46131"/>
        <property type="gene ID" value="AFR_39385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7968"/>
    <dbReference type="eggNOG" id="COG4770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_2_11"/>
    <dbReference type="OrthoDB" id="361205at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0092">Biotin</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="11">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="10">
        <location>
            <begin position="119"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="503"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="481"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ46131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="581" mass="61602" checksum="096C5DA6BEB0B89E" modified="2014-01-22" version="1">MRKVMIANRGEIAVRVIRACKDAGLTSVAVYADADRDGLPARLADEAYALDGDTATETYLRIDKLLDIAARSGADAVHPGYGFLSENADFAQAVQDAGLTWIGPTPQAIRDLGDKVTARHIAQRAGAPLVPGTAEPAANADEIVAFAREHGLPVAIKAAFGGGGRGLKVARTLEEIPALFESATREAVAAFGRGECFVELYLDRPRHVEAQVLADTHGNVIVVGTRDCSLQRRHQKLVEEAPAPFLTDDQRARIHSSAKAICREAGYHGAGTVEYLVGQQGTISFLEVNTRLQVEHPVSEETSGLDLVREQFRIADGGELALTEDPAPRGHSIEFRINGEDAGRGFLPAPGQVTALTWPSGPGVRVDAGVEAGSVIGGNFDSLLAKIIVTGADRTEALERARRVLDETVIEGLATVLPFHRLVVRDPAFVDFTVHTRWIETEWQGGVDAFTPAATAPLETEERETVVVEVAGKRLEVRLPANLRSGTRTGAPSRPRARQERAATKAGGASGAAALTAPMQGTIVKVAAHNGDTVAEGDVIVVVEAMKMEQPLQAHRAGTVSELTVEVGATITAGTTICEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDZ6</accession>
    <name>U5WDZ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562475.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39435"/>
    <dbReference type="EnsemblBacteria" id="AGZ46141">
        <property type="protein sequence ID" value="AGZ46141"/>
        <property type="gene ID" value="AFR_39435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7978"/>
    <dbReference type="eggNOG" id="ENOG50339C4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087212_0_0_11"/>
    <dbReference type="OrthoDB" id="1438002at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26092" checksum="7A65CBF34AAB0BA6" modified="2014-01-22" version="1">MTPEQAAARAKPGLAKLVGAFAESPQTLRRARLMGLSGWAYHVSARAGALGDVRPETVAAAIGFIAPDAVTDGWEAAAKTTPPVEVATWHLHELCRWGVDELGGFPRLSRLLELSKRVVSAVDAAGLPLFAAWRAMPVPETSPGAQAAVMLHLLHEHRLGVHVVAVRASGLTPLQAIMAGPDGETDAVAFGWQPPYPSPGPIVRRLMWAESVADSLTGQAYAQLDRAERVELCGLLESLSHRLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEI5</accession>
    <name>U5WEI5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562787.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41005"/>
    <dbReference type="EnsemblBacteria" id="AGZ46455">
        <property type="protein sequence ID" value="AGZ46455"/>
        <property type="gene ID" value="AFR_41005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8300"/>
    <dbReference type="eggNOG" id="ENOG5030WPT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_569421_0_0_11"/>
    <dbReference type="OrthoDB" id="1061345at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="439" mass="46480" checksum="7ABAD5A104CA3A97" modified="2014-01-22" version="1">MIRLLGDEMLQWSDPAPVRGEALFPLVAVAHGRTLIAGPHGPALIGQVPADDLTLLVRGLTDGEALAARFPAATVCVGGLAKFTAGPPFDTVIALAGLEPLTSAEGVELSWGESLDLLLGLLRPGGTLLLGVENFLGLHRLVALPSRPTDADWAPVAEHDPTRPRGLRRVRDRVGVATSAFAVYPSLSAPTLVLDADDLDREEHLGALSALITRAVEPSAPVLTDPRRLAVETLRHGTAADVAPAWIVTAGVRVDLARALPSGRTLQDLLLEAALGRDLPTVRELLGRWWDGPCAGVPADQIVVGSEWVALAPAGDHGAALLRFATDLIDGGYPHPWPAPPGPADLAVTLAAMTGRTIDPPAPADRPDLVLRDLVAARDRLTAELAEARAKHLWYEQMLVSREQALQRARYLLGASAPVRAVVSGARAAKRTLRVLRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEZ1</accession>
    <name>U5WEZ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Peptidase S8/S53 subtilisin kexin sedolisin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AFR_41580</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41580"/>
    <dbReference type="EnsemblBacteria" id="AGZ46570">
        <property type="protein sequence ID" value="AGZ46570"/>
        <property type="gene ID" value="AFR_41580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8413"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_13_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023834">
        <property type="entry name" value="T7SS_pept_S8A_mycosin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03921">
        <property type="entry name" value="T7SS_mycosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666531" evidence="13">
        <location>
            <begin position="31"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="14">
        <location>
            <begin position="82"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ46570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="435" mass="44301" checksum="DD2080F8A1BDF9F8" modified="2014-01-22" version="1">MNVRSLPVRLAATTAAAGLCVGLSGTPVLAETGGQPPQAVPLASPFASPLASPEPWVGDSVRAEQWHLKTLNVAGAWTYSMGAGVTVAVIDSGVDSDHKDLQGQVLPGLDLVDPDGDGDTDLVGHGTTVSAIIAGRSDDDAGVVGIAPKAKILPVRVLDRENRYDDAMIVAKGVRWAVDHGARVINLSLGGNGSSPALAAALDYAFAKDVVVVACTGNASTSSSSGVWYPAREPGILAVAGMEKDGDILWSGSITGKETVVTAPATQLVGARPDGYWRVQGTSFAAPMVSGTAALIRSRWPTMSAGEVINRIIKTAKDRGATGRDPQYGYGMVDPTGALTAELPTVLRNPLDTTASPGIARFGNAPISGQAQSAPDSAAAGTVGTRLPGLNAGTAARAGDPAATGSNHGWWAAGLLFLLSVVAAIFTVRRFATIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF57</accession>
    <name>U5WF57_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562996.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42055"/>
    <dbReference type="EnsemblBacteria" id="AGZ46665">
        <property type="protein sequence ID" value="AGZ46665"/>
        <property type="gene ID" value="AFR_42055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8510"/>
    <dbReference type="eggNOG" id="COG3315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067079_1_0_11"/>
    <dbReference type="OrthoDB" id="1186918at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006764">
        <property type="entry name" value="SAM_dep_MeTrfase_SAV2177_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04672">
        <property type="entry name" value="Methyltransf_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017393">
        <property type="entry name" value="MTase_SAV2177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="270" mass="29630" checksum="B4673DBAC335D592" modified="2014-01-22" version="1">MTQESAEVAPPGVNINVPHSARIYDYWLGGKDNFAVDRAVGEAMMKAIPAMRYMAGENRKFVHRAARDLVAKEGIRQFLDIGTGIPTRPNLHEIAQKLAPETRVVYVDNDPIVLVHARALMISTDEGRSEYISADIRKPQSILTEQVLTETLDLTEPVGLTLIAILMLLADADDPWSLVAQLRDAMPSGSILGITHPTADFNPEAVGEAVSAATSAGMTLVARTREEVERFFGDWEMLEPGLVPVSAWRPDEPVENPEAAYYWAGVARKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFS6</accession>
    <name>U5WFS6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the fructosamine kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563117.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42660"/>
    <dbReference type="EnsemblBacteria" id="AGZ46786">
        <property type="protein sequence ID" value="AGZ46786"/>
        <property type="gene ID" value="AFR_42660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8633"/>
    <dbReference type="eggNOG" id="COG3001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036517_0_2_11"/>
    <dbReference type="OrthoDB" id="1457558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016477">
        <property type="entry name" value="Fructo-/Ketosamine-3-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12149">
        <property type="entry name" value="PTHR12149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03881">
        <property type="entry name" value="Fructosamin_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006221">
        <property type="entry name" value="Ketosamine-3-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="31614" checksum="C9135564D914D242" modified="2014-01-22" version="1">MDLAYLRAHPQHLPTFLTHQRIRETPVGGGDVCTASRLTLDDGASLFAKTWPSGSAPEHFFATEAAGLEWLREAGAVPVPEVLVALPEMLALEWIDSGPATPGAAERFGRELAAMHRSGADSFGASWPGFLGPLPQDNTPSEGPWGAWFAERRLRPYLKISADSNALSAADIAVVEQVIHKIEGYAGEEPPARIHGDLWPGNVLWAADGRAWLIDPAAHGGHRETDLAQLALFGGAPHLDRIISAYEEEWPLADGWRSRVAWHQLHLLLLHTAAFGGSYRGAVRAAADSALVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VN88</accession>
    <name>U5VN88_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="3 12">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">purM</name>
        <name evidence="16" type="ORF">AFR_00745</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="3 12"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00745"/>
    <dbReference type="EnsemblBacteria" id="AGZ38438">
        <property type="protein sequence ID" value="AGZ38438"/>
        <property type="gene ID" value="AFR_00745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.150"/>
    <dbReference type="eggNOG" id="COG0150">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_11"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="14">
        <location>
            <begin position="88"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="15">
        <location>
            <begin position="204"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AGZ38438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="380" mass="40313" checksum="5D17C798CB56417A" modified="2014-01-22" version="1">MSERNSAGSSGAEGADHQPWSAGAGRSGRKRTVTYADAGVSIHAGERAVELLKSKVKKTTRPEVMGDLGGFAGLFKLNTQKYKSPILASSTDGVGTKLVIAQQLDIHDTIGIDLVAMVVDDLVACGAEPLFLLDYIACGEVVPDKVAEIGAGIADGCRYAGCALLGGETAEHPGVLRPDEYDVSATGVGVVEESEILGAHRVEIGDAVIAMRSSGLHSNGYSLVRHVLLGAGRMRLDTVMEDFGRQRTLGEELLTPTKIYAKDCLGLIEETDVRAFSHVTGGGIPGNLVRILPEHVDAVVDRSSWRPQPIFDLIQAKGRIEDVEMEATFNMGVGMFAIVSSDDADRAMAYLTGRGVEAWQVGEVIEGTGQVQMVGQYTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNG8</accession>
    <name>U5VNG8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00265"/>
    <dbReference type="EnsemblBacteria" id="AGZ38342">
        <property type="protein sequence ID" value="AGZ38342"/>
        <property type="gene ID" value="AFR_00265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.54"/>
    <dbReference type="eggNOG" id="COG1716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047963_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022128">
        <property type="entry name" value="FhaA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042287">
        <property type="entry name" value="FhaA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032030">
        <property type="entry name" value="YscD_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12401">
        <property type="entry name" value="DUF3662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16697">
        <property type="entry name" value="Yop-YscD_cpl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SSF49879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="179"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="252" mass="27215" checksum="02515E1B5A4BB13C" modified="2014-01-22" version="1">MSVLQRFEKRLEGLVEGAFAKVFKGVVHPVEILNAMQREAEAHKAILAGGRTLVPNRYVIDLSPYDHSRLAPYAAALAQELAQSQAEFIGEQAWTVYGDVIVEIERGDGLDTGMFRVTAEVYTGGEVAPVQQPAYDAAPAYSPYDQQQQGGYQQHGHNGRNVGLVSGDGRTYPLQMGSTVIGRGDQANLRLPDVGISRRHARLDYDGSQVVLTDLGSTNGTMVNGQRVSAVALNPGDMIQLGTTTLTFRVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP52</accession>
    <name>U5VP52_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_02270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 65 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357686.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02270"/>
    <dbReference type="EnsemblBacteria" id="AGZ38743">
        <property type="protein sequence ID" value="AGZ38743"/>
        <property type="gene ID" value="AFR_02270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.459"/>
    <dbReference type="eggNOG" id="COG1554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006285_2_2_11"/>
    <dbReference type="OrthoDB" id="179460at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005194">
        <property type="entry name" value="Glyco_hydro_65_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005195">
        <property type="entry name" value="Glyco_hydro_65_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005196">
        <property type="entry name" value="Glyco_hydro_65_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037018">
        <property type="entry name" value="Glyco_hydro_65_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017045">
        <property type="entry name" value="Malt_Pase/Glycosyl_Hdrlase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03633">
        <property type="entry name" value="Glyco_hydro_65C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03632">
        <property type="entry name" value="Glyco_hydro_65m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03636">
        <property type="entry name" value="Glyco_hydro_65N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036289">
        <property type="entry name" value="Glycosyl_hydrolase_malt_phosph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_65N" evidence="7">
        <location>
            <begin position="16"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_65m" evidence="5">
        <location>
            <begin position="327"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_65C" evidence="6">
        <location>
            <begin position="700"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="361"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="592"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="764"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR036289-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR036289-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="788" mass="87408" checksum="A2F97D762C53C1E5" modified="2014-01-22" version="1">MIRERAYPVDPWHIRETRLDLDLLAQSESVFALANGHIGIRGNLDEGEPHGLPGTYLNSFYELRPLPYAEAGYGFPESGQTMVNVTNAKLMRLLVDDEPFDVRYGELRSHERTLDLRAGTLERIVEWVSPSGQAIRVRTVRMVSFTQRAVVAFLYEVEPLTSSARLILQSELVANEQLPPTSKDPRVAAVLDHPLQAEEMLEQRTGGLLVHRTKASDLRMAAAMEHLVEAPGKHAITTEGHPDWLRTTVATKLEPGQKLRVVKLAAYGWSSNRSLPAVRDQVGAALASARLDGWEGLVEAQRDYLDAFWDHSDVKVEGDPEVQQAVRFGLYHTLQAGARAEQRPIGSKGLTGPGYDGHTFWDAETFVLPALTYTQPAAAADVLRWRHSTLDLARERAQTLGLQGAAFPWRTIRGQECSAYWPAGTAGFHIGADIADAVRRYVSATGDYDFERDYGLELLVETARLWRSLGHHDRHGRFHIDGVTGPDEYTAVVDDNIYTNLMAQQNMFAAADAAKRHPDLARKLGVDDEESASWRDAAASVHIPYDRELGVHQQSEGFTRLQEWDFQNTPPEVYPLLLNYPYFDLYRKQVVKQADLVMAMYVRGDAFTPEEKARNFAYYEARTVRDSSLSACIQAVMAAEVGHLELAHDYLGEAALMDLHDLHQNARDGVHVASLAGSWIALVAGLGGMRDSNGQLSFAPRLPSRINGLEFSLLWRGLRLRVEVTADEVTYSLRNGGGSARLDLLHHGKEVTITQVKPVVLPIPPQGPMGPAPTQPAGRAPVRRATGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPC9</accession>
    <name>U5VPC9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01865"/>
    <dbReference type="EnsemblBacteria" id="AGZ38662">
        <property type="protein sequence ID" value="AGZ38662"/>
        <property type="gene ID" value="AFR_01865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.377"/>
    <dbReference type="eggNOG" id="ENOG5033A2X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116311_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012495">
        <property type="entry name" value="TadE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07811">
        <property type="entry name" value="TadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="127" mass="12682" checksum="CB440C2508779713" modified="2014-01-22" version="1">MTGRRWPGRLRRLTSDRGAFTAELAAGLPALMLLLFAGLTAVGAVTAKIQCVDAAREGALIAARGGAGDQDALRIAPSGAAVAVSEGQETVTVTVRAPVPILGGFLSAITVRATAVASLEPDGTGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPP6</accession>
    <name>U5VPP6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357717.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02425"/>
    <dbReference type="EnsemblBacteria" id="AGZ38774">
        <property type="protein sequence ID" value="AGZ38774"/>
        <property type="gene ID" value="AFR_02425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.490"/>
    <dbReference type="HOGENOM" id="CLU_712965_0_0_11"/>
    <dbReference type="OrthoDB" id="1908699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="SSF50969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="35175" checksum="F7D3457F7E33B13C" modified="2014-01-22" version="1">MRVETAIRAAAQATGGYPGSLDDVYRRVRDRRRRRRTAAGACAGVVVVLLGIGVSVNRAAEPAPPPAAVPDVAVAQRLMLDGAAGSYTGAGGATVSLGEEDAVGELLPDGRLVAHRVVGADAWNHVVGTPDGRVVAIGPRNLVRGVPMALRDQLVVVGPTGSTEVIREAGQAGAPLTLVGATSTTAYLWRRTGLVAHDLSTGNELALVPADRVGTIVAADVAGDRLAAVPRSSRCRPTVLDTSTDDVVAWTDLSPLRCDRVLGLRLSPDARSLAVAYQADDGHRVVIVSLDGGKVQADQEIPTAPRVALAWQDDRKLRVATFAAGPAQQVRAFTITAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQR0</accession>
    <name>U5VQR0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_04215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358077.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04215"/>
    <dbReference type="EnsemblBacteria" id="AGZ39132">
        <property type="protein sequence ID" value="AGZ39132"/>
        <property type="gene ID" value="AFR_04215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.851"/>
    <dbReference type="eggNOG" id="COG4320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032121_1_0_11"/>
    <dbReference type="OrthoDB" id="571864at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018721">
        <property type="entry name" value="DUF2252"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39441">
        <property type="entry name" value="PTHR39441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10009">
        <property type="entry name" value="DUF2252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="434" mass="47998" checksum="77D9A0913FF8E2C3" modified="2014-01-22" version="1">MSEQSRADFIIDVLTREFGALMALDPAAFRRKFRKMAASPFAFYRGSASLFYADQTGAYRDDRFLDEHTSRVWIHGDLHAENFGTYMNSSGQLVFNVNDFDEAYVGPFIWDLKRFSASVALIGYSKALSDDVISQLVTTFAQAYLTELRAIAAGGDDAIGSITLENADGVLRRVLQEARLNTRVDLLAGQTVIDDYERRFSLGDGVYDVDEETAATVTAAFKDYLETIPSHGASVSTTIKDIKLRKGVGIGSAGLPSYNMLLEGHTQALENDVIIYMKQAQVPAVARWVDDERVRGYFKHQGHRTAESQRALQAHADPWLGYTELNGVGQLVAEVSPYAADLDWADVNEPEELTGVIADLGRAVARMHSVADDESSHDLVDFSTEEAIVAEVDKDEAGFVAVLVDFAHKYGDQAREDHQDFVDLFRNGRLPGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRL9</accession>
    <name>U5VRL9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_06495</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359119.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06495"/>
    <dbReference type="EnsemblBacteria" id="AGZ39588">
        <property type="protein sequence ID" value="AGZ39588"/>
        <property type="gene ID" value="AFR_06495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1317"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_5_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="33"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="120"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="179" mass="19328" checksum="053DA2AC9D05075C" modified="2014-01-22" version="1">MIAADPLTSLALRARAGDAGAQAAFVRGTQAEVWRFTAALVDPGAADDLTQETYLRAFKALPGFEGRAQVRTWLLGIARRTCADHLRAVVRRRRLDARLAAQAWTDIPQQGDPAHRLSAADLLSRLGEERRTAFVLTQVLGLSYAEAAEVENVPVGTIRSRVARAREELVTAVAAAQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRN2</accession>
    <name>U5VRN2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_00135</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357260.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00135"/>
    <dbReference type="EnsemblBacteria" id="AGZ38316">
        <property type="protein sequence ID" value="AGZ38316"/>
        <property type="gene ID" value="AFR_00135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.28"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="285" mass="30689" checksum="4523F2EC563571FE" modified="2014-01-22" version="1">MTTVRITGLGKRYAATTALDDLSLEIGTGVTGLLGPNGAGKSTLLRCLATALAPDTGRIEAFGLDPAVAIQRTELRRRLGYLPQNPGLYPNFTAAALLDYVAVLKEIVDGPQRRAEVRRVLEEVGLTDRAKTRVRKLSGGMRQRLGLAQALLGDPSLLILDEPTVGLDPEQRMGFRALISRLGETRTVLLSTHQTEDVGALCERVVVMRRGSAIFDGTPRALSGLAQGQVWLSTEPPPAHQMYWRTSDGDYRTLGPQPPQAEAAKPSLEDGYLMLLGPEVAAAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRS6</accession>
    <name>U5VRS6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dCTP deaminase, dUMP-forming</fullName>
            <ecNumber evidence="3">3.5.4.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Bifunctional dCTP deaminase:dUTPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">DCD-DUT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">dcd</name>
        <name evidence="6" type="ORF">AFR_00385</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dCTP + 2 H2O = diphosphate + dUMP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19205"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.5.4.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dCTP deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.30" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357310.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00385"/>
    <dbReference type="EnsemblBacteria" id="AGZ38366">
        <property type="protein sequence ID" value="AGZ38366"/>
        <property type="gene ID" value="AFR_00385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.79"/>
    <dbReference type="eggNOG" id="COG0717">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_2_1_11"/>
    <dbReference type="OrthoDB" id="1598407at2"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00667"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033973">
        <property type="term" value="F:dCTP deaminase (dUMP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase" evidence="5">
        <location>
            <begin position="60"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="101"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for bifunctional activity" evidence="3">
        <location>
            <begin position="116"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="21307" checksum="A7151797C3969848" modified="2014-01-22" version="1">MLLSDRDLVSEIKSENLALEPFEPELMQPSSIDVRLDRFFRVFNNHLYTHIDPAEQQDDLTAQVEVVDGQPFVLHPGEFVLASTLEVITLGDQLAGRLEGKSSLGRLGLLTHSTAGFIDPGFSGHVTLELSNVANLPIKLWPGMKIGQLCIFRLSSPAEHPYGSAVYGSRYQGQRGPTQSRSHHNFRTWPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRY4</accession>
    <name>U5VRY4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Prim-Pol domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359125.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06525"/>
    <dbReference type="EnsemblBacteria" id="AGZ39594">
        <property type="protein sequence ID" value="AGZ39594"/>
        <property type="gene ID" value="AFR_06525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1323"/>
    <dbReference type="eggNOG" id="COG3378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057861_2_0_11"/>
    <dbReference type="OrthoDB" id="1173531at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015330">
        <property type="entry name" value="DNA_primase/pol_bifunc_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09250">
        <property type="entry name" value="Prim-Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00943">
        <property type="entry name" value="Prim-Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prim-Pol" evidence="1">
        <location>
            <begin position="13"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="33267" checksum="9977AA1BD5DDDB04" modified="2014-01-22" version="1">MPVSCLDSLRAAALSAAARGWHVFPLRPGDKRPAFPDHAEDRCARTDLRCRTGHTGWEGRATTDPQRITRAWQRIPYNIGIACGPSRLIVIDLDMPKPGTVLPAEWVTEGVRDGGDVFAVLCQRAGQPFPNTYTVSTASGGTHLYFRHPATGSPLRNTRGESGRGLGPLIDTRAHGGLVVAAGSVIGAHTYTVLDDTAPELLPRWLAEPLYPEPPRPAVPVVLGADRAGRYVRAAIDRTLTKLAGASEGGRNDALWMAAQSLGQLAAGGALTDQQVTDALEPAARSLGLTARETARTIRSGLTAGARNPRSVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSA8</accession>
    <name>U5VSA8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359275.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07275"/>
    <dbReference type="EnsemblBacteria" id="AGZ39744">
        <property type="protein sequence ID" value="AGZ39744"/>
        <property type="gene ID" value="AFR_07275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1485"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051796_0_0_11"/>
    <dbReference type="OrthoDB" id="1694750at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="37"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="37861" checksum="280D4106F117F89F" modified="2014-01-22" version="1">METDVLGLPYEKHTIDLGTDDEGPVIATLVRRRGDTPSRRAVLYVHGFVDYFFQTHLADFFVERGWDFYALDLRKYGRSLLPHQTPNFCRSLTDYYPELDESARIIREQDGHDQLLVAGHSTGGLITSLWSHSRSNEGLVDGLFLNSPFFDFNAPWLLRRPIANIVGRASGRKPYRVLPLSTLGLYGQSLHSDHRGEWTYDLAWKPVNGFPVRTGWLEAIRRGQARLRAGLTIDAPVMVACSTRTFRGRGWTEDVRLSDAVLDVEHIARWAPRLGSRVTIARFDGGLHDLTLSGKDVRVEVFQELGRWVDAFVPPKGTPEIEVPPAPEDKADATAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSB4</accession>
    <name>U5VSB4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">DNA repair protein RecO</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Recombination protein O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">recO</name>
        <name evidence="10" type="ORF">AFR_07995</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in DNA repair and RecF pathway recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the RecO family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359419.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07995"/>
    <dbReference type="EnsemblBacteria" id="AGZ39888">
        <property type="protein sequence ID" value="AGZ39888"/>
        <property type="gene ID" value="AFR_07995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1632"/>
    <dbReference type="eggNOG" id="COG1381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066632_1_1_11"/>
    <dbReference type="OrthoDB" id="1758685at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00201">
        <property type="entry name" value="RecO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022572">
        <property type="entry name" value="DNA_rep/recomb_RecO_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003717">
        <property type="entry name" value="RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042242">
        <property type="entry name" value="RecO_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33991">
        <property type="entry name" value="PTHR33991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02565">
        <property type="entry name" value="RecO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11967">
        <property type="entry name" value="RecO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="SSF57863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00613">
        <property type="entry name" value="reco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_rep/recomb_RecO_N" evidence="9">
        <location>
            <begin position="12"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="28184" checksum="F05906B6937A7CF7" modified="2014-01-22" version="1">MTSVPTGYRRHLYRDDAVVLRVQKLGESDRIITLLTRRHGRLRAVARGVRRTTSRFGARLEPFGHIDLQLAGSPEGVGSSLHSVSQVEALTLFGKRFLEDYPRYTAASAIAETAERLSPVEREPALRLFQLTLGAFKALAAGEHASSLVLDAYLLRGMNFAGWAPALGECAVCGDPGRHGAFSVPAGGAVCPDCRPPGAAHPAPATIGLMQALTSGDWQLADSTEPSARRECSGLVAAHLQWHMERNLRALPLVDRRESTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSG0</accession>
    <name>U5VSG0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357504.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38561">
        <property type="protein sequence ID" value="AGZ38561"/>
        <property type="gene ID" value="AFR_01360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.272"/>
    <dbReference type="HOGENOM" id="CLU_095971_0_0_11"/>
    <dbReference type="OrthoDB" id="1732383at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="79"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="19430" checksum="8ACE767D54AA0EC5" modified="2014-01-22" version="1">MTSTDVPGGGRHRARFPASGTFAEPAGTAGRPSSASLAREHELARMRAKLMDPETAARDLAERLTFAHVNAGKPALSVLGEAVHYSKATLSKVFAGKMVPSWPLVEDLAVALRVPPQTVVQEWLLLWTAANTLRRNPDAGRPAPGTTAATTDAGYTCPKCGSWVVDTALHTGWHLQISDPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSM0</accession>
    <name>U5VSM0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADH-quinone oxidoreductase</fullName>
            <ecNumber evidence="9">7.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_01685</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="9">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357569.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01685"/>
    <dbReference type="EnsemblBacteria" id="AGZ38626">
        <property type="protein sequence ID" value="AGZ38626"/>
        <property type="gene ID" value="AFR_01685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.339"/>
    <dbReference type="eggNOG" id="COG1034">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_4_0_11"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 8 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0874">Quinone</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="10">
        <location>
            <begin position="9"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="12">
        <location>
            <begin position="89"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="11">
        <location>
            <begin position="246"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ38626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="824" mass="86857" checksum="73B88C648CAF6603" modified="2014-01-22" version="1">MTDVAKKTDTVTLTIDGVEVTAAKGELVIRVAERMGTAIPRFCDHPLLAPAGACRQCLVEVEGQRKPVASCTQTVAEGMVVKTQLTSPVAEKAQGGVMELLLANHPLDCPTCDKGGECPLQNQAMSSGRVDSRFHEHKREYEKPIHISSQVLLDRERCILCQRCTRFSEEIAGDKFIDLMDRSSGEQINVYRDDFFGGEGFGDGQAGDGEGDVPFNSYFSGNTIQICPVGALTGEQYRFRARPFDLVSSPSVCEHCAGGCASRVDHRRGKVLRRLAGDDPAVNEEWNCDKGRWAFRYATAHDRIMNPLIRDAGTGELREASWSEALLAAAEGLQAAKGKGVGVLTGGRLTVEDAYAYAKFARVALGTNDIDFRARPHSAEEADFLAASVAGVTDVTYEDVENATSAVIVGLEPEEEAAILFLRLRKGALKKKLKVTAVAPYLSRGFEKLNAALVTALPGDEARLLDTDGTIAAALAAPGALLIVGERLVETPGGLSSAAKLAERTGARLAWIPRRAGDRGAVDAGCLPNLLPGGRPVTDEQARGELARAWDVDSAVLPGTSGRDGDAIIAAAAAGELGGLLVAGVDPADLTDPRLAEQALDAVPFIVSLELRQSAVTRRADVVFPIAPAAEKPGTFINWEGRLRTFETVLNTTAMTDGRVLEAVAALMDVTLGTGDVGAIRRELGAMPASRSARPTLPAVEPVAVRKPSAQEALLATWHQMLDLGSLQDGDEALAGTARPSVVRLGKGLAESLGVADGDAVTVSTDRGGITLPAAITDLPPQVVWLPTNSPGSTLRRSLGVTSGAVVRLSAGKPGPILAQGANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT51</accession>
    <name>U5VT51_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_03080</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357848.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03080"/>
    <dbReference type="EnsemblBacteria" id="AGZ38905">
        <property type="protein sequence ID" value="AGZ38905"/>
        <property type="gene ID" value="AFR_03080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.620"/>
    <dbReference type="eggNOG" id="COG3537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003690_2_0_11"/>
    <dbReference type="OrthoDB" id="100818at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005887">
        <property type="entry name" value="Alpha_mannosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041371">
        <property type="entry name" value="GH92_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012939">
        <property type="entry name" value="Glyco_hydro_92"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07971">
        <property type="entry name" value="Glyco_hydro_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17678">
        <property type="entry name" value="Glyco_hydro_92N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01180">
        <property type="entry name" value="aman2_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665663" description="F5/8 type C domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="3">
        <location>
            <begin position="561"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="952" mass="100317" checksum="BF9461B4B905BC7D" modified="2014-01-22" version="1">MIGFRTVAALAGVALAVPIGAGAQAAAPPPLVAEPATYVNPFIGSTNLGNTYPGAVTPFGMLAWSPQTSRGTQISTPAPGGYQYTATRIRGLSLTHLSGVGCSGANGDIPIMPHVGDITTSPTADATDAVFASTFSHANEAAKPGWYKVGLDSGAGAELTVTPRTGFGKFSFPADKPAGLLFRTSMSETGSEAATVHIDPATQTVTGSVSAGNFCGPQSTNNSKAYYTLHFVAKLDQPFSATGTWKDATLAPGSTDAQGGSGYSSTGRPNAGKGSGGYVSFAPGTTSVGMRVAISYVSLDGAKANLAQESPERLSFAAIQAGARSAWSARLKKIGIGGGTEDQKTTFYTALYHAMLEPTLTSDVDGRYYGADDAVHTLSTGQKAQYGTFSGWDVYRAQVQLLALLDPKMASDYAQSLFNYANQRDGEWDRWLLQHGKTAVMSGDPAAAAVAGMYAFGARDFDVQGAFDSLVHAATVPTANDSSDAGCNVECVGQRPSLDKYLELGYVPADNCHCWGGAAETLEDAAADYGIAELAKALGEKEKHADFLDRSHNWRNVYDKDAKADAGLEHNIRERITEITASAENPPGEGKTQVFDGDKGTKWLTFATTGWVQAKLAAPLTVEKYALTSANDVPERDPRDWQLQGSTDGTTWTTLDTQTGQAFEKRGQTKEYTVANPQPYSYYRLTITANGGAPIVQLAEVELADPAVPTPIPADGAFVGWMRDRYADGDWAEGFSPSTGSGFVEGSSAQYTWMVYSDVVGLAAAMGGNKVAIERLDAFFRNPDGTFDLSATKGTKFDATNEPDIQTPYLYNYFGAAYKTQETVRAIVDGKWSNGTGGIPGNDDAGTMSAWYVFSALGLYPTVPTRAELALTTPLFPRAVVHLANGKDLTIEAPRSDAKAIYVEKLTVNGKTTTKGWLPASVVSTGGKLSYTLSTTPSLTWGSGPGDVPPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT91</accession>
    <name>U5VT91_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alkyl hydroperoxide reductase/ thiol specific antioxidant/ mal allergen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_08800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359580.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08800"/>
    <dbReference type="EnsemblBacteria" id="AGZ40049">
        <property type="protein sequence ID" value="AGZ40049"/>
        <property type="gene ID" value="AFR_08800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1791"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_11"/>
    <dbReference type="OrthoDB" id="892697at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="3"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="3">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="154" mass="17271" checksum="C1B1FB38227A0BAD" modified="2014-01-22" version="1">MPIDVGTQAPDFTLKDQNNQEVSLADFKGRKAVLLVFYPLAFTGTCQGELAEIRDNLSQYENDYVQVLTVSVDSAYSHKVWADREGFTFPMLADFWPHGGVAQAYGVFFEQAGIANRGTFVIDKEGVVRYAHMQGPGEPRDQNTWRDTLAKIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTA0</accession>
    <name>U5VTA0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359851.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09570"/>
    <dbReference type="EnsemblBacteria" id="AGZ40203">
        <property type="protein sequence ID" value="AGZ40203"/>
        <property type="gene ID" value="AFR_09570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1951"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051715_2_0_11"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="37"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="27208" checksum="A9104A95121EF56C" modified="2014-01-22" version="1">MSPQLLRFADWTAPVTAVEREVLHALPESPSDESPPVLFVPGLGHGAWAFAEHWLGHTASRGFAAHAVSPRADGSLRAYVHDVVQVAASLPRQAVLVGHGAGALVVSHALARYPARAAVLASPVPAGWAAVGAALRANPLGTLPALFGAAPRLSRKQLFSAGLAEGEAYQERLVRAPARVYRQVLFAKPAAEPVGAPPTLVVGSPDDRVVPRAALDRAAARFGGAPLLFPGMGHDLMLDTDWREPIDAILDWLGKELAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTC4</accession>
    <name>U5VTC4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative D-alanyl-D-alanine carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359626.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09000"/>
    <dbReference type="EnsemblBacteria" id="AGZ40089">
        <property type="protein sequence ID" value="AGZ40089"/>
        <property type="gene ID" value="AFR_09000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1831"/>
    <dbReference type="eggNOG" id="COG1876">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116589_0_0_11"/>
    <dbReference type="OrthoDB" id="1706034at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003709">
        <property type="entry name" value="Pept_M15B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02557">
        <property type="entry name" value="VanY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VanY" evidence="1">
        <location>
            <begin position="97"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="202" mass="21528" checksum="DA7336043923F4CB" modified="2014-01-22" version="1">MGIEAVNSRIADIQSRMIALQTQQATATKQATSATSTTSGSGSTFATALADAMGTQSSAGTAKSFSLNSKGIPTELAAYGNGKIPANALEKVGTTNHKLWAPAAENLNRMISDAKAQGVKIGITDSYRSYDEQVDVARRKGLYSQGGLAAKPGTSEHGWGMATDLDLNNKAQAWMRENGEKYGFVENTPREPWHWAFKPKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTV9</accession>
    <name>U5VTV9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lon N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360060.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10100"/>
    <dbReference type="EnsemblBacteria" id="AGZ40309">
        <property type="protein sequence ID" value="AGZ40309"/>
        <property type="gene ID" value="AFR_10100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2057"/>
    <dbReference type="eggNOG" id="COG2802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048359_1_1_11"/>
    <dbReference type="OrthoDB" id="1474822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="25998" checksum="4292974DBE9D14B7" modified="2014-01-22" version="1">MGAIIPVFPLGTVLFPGLVLPLHIFEERYRSLVRDLVASTDDEPHEFGVVTLRHGWEVAAPDGDAERPADETPITVDQLYAVGCTAELRQVTELPDGRFDIVTVGRRRFRVVAITADTQPYVTAEVEWLPDEEVTDPVSTLLAPRALAAFRTYLDLLRPDSEVLDQVPDDPTVLSHLIAATAQLTTDERQELLAAPDTATRLRTELRLLNREAGLLAKVRAVPVPLSDLARPVSPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTY3</accession>
    <name>U5VTY3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358021.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39076">
        <property type="protein sequence ID" value="AGZ39076"/>
        <property type="gene ID" value="AFR_03935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.795"/>
    <dbReference type="HOGENOM" id="CLU_1881299_0_0_11"/>
    <dbReference type="OrthoDB" id="2150189at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="135" mass="13799" checksum="8B276CCDE21C6F77" modified="2014-01-22" version="1">MNNASAPYLLEGLAGLSPAQRRQAVATVVFRVWETPAVLLGEIPPTQALTAIAAIAAGLPGAAGLPWVDDRLLAAACPNVTSELAGRALSALDVIVGRDSASVPLELHVTDGEAAGALLNDLRDVLMDGLRLGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU86</accession>
    <name>U5VU86_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IMP dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_04955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358339.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04955"/>
    <dbReference type="EnsemblBacteria" id="AGZ39280">
        <property type="protein sequence ID" value="AGZ39280"/>
        <property type="gene ID" value="AFR_04955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1001"/>
    <dbReference type="eggNOG" id="COG0516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064068_0_0_11"/>
    <dbReference type="OrthoDB" id="532857at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005992">
        <property type="entry name" value="IMP_DH-rel2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF85">
        <property type="entry name" value="PTHR11911:SF85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01304">
        <property type="entry name" value="IMP_DH_rel_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMPDH" evidence="2">
        <location>
            <begin position="17"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="372" mass="39659" checksum="65CF41634E7A1521" modified="2014-01-22" version="1">MRDVVEIGLGKTAQRGYHLDDIAIVPSRRTRDVDDVSTEWRLDAYPFKIPCVAHPSDATMSPQSAVALGRLGGLGVLNAEGLWTRYEDPTKILEELASLDEEADATKRLQEVYSEPIRPELIAERVRVMREGGVTVAVRVSPQHTLALAPVILDAGVDLLVIQGTLVSAEHVSTTDEPLNLKEFIADLDLPVIVGGCTDYKTALHLMRTGAAGVIVGIGADEWSTTDTVLGIRVPMATAIADAAAARRDYLDETGGRYVHLIADGGIQTSGDIAKAIGCGADAVMLGEPLSLAEGAPAGGAWWHSAASHPKLPRGGFCIAGEPDGSLEELLYGPANRPDGQLNLFGGLRRAMAKCGYHDVKEFQKVSLVLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUQ3</accession>
    <name>U5VUQ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05880"/>
    <dbReference type="EnsemblBacteria" id="AGZ39465">
        <property type="protein sequence ID" value="AGZ39465"/>
        <property type="gene ID" value="AFR_05880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1194"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_962254_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="194"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28693" checksum="8A7AABD4CB30F7F2" modified="2014-01-22" version="1">MQMALDVAAVAAECRTVGEVQSQLLPALAAATGAEIAVYHQIALGPELEEYGVPWPDHDSFATVLEPYPAVMHESPLIRHFVRHPGPGVIGVSELVSRREWRENAVYRESLRHLGFGDQLALVFSLNDGNGHAITLGRQGRAFSRREGDLLRLVEPHVRAAVRRSLEAPDAYRVVHTRRQRVRWAVRSGPAIAAAGTAPALTGRELEVLALVAGGLSNQQIGRRLGLAPRTVDKHLENAFGKLGAHSRVEAVVNLRVRMAHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUW0</accession>
    <name>U5VUW0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12460"/>
    <dbReference type="EnsemblBacteria" id="AGZ40778">
        <property type="protein sequence ID" value="AGZ40778"/>
        <property type="gene ID" value="AFR_12460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2531"/>
    <dbReference type="eggNOG" id="COG0699">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031445_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="483" mass="52514" checksum="7B51A76CBAE04879" modified="2014-01-22" version="1">MIDDEDLEQRVQRLLSQATHAYRGNRAAAGVLQEAATRLVEPLRVVVAGTPNSGKSTLVNALIGEDVAPAAPTDGPSAFTSYQDGPAPRAWWFAEDQRPHEVAMVRTTPGLRLSTGAAQRSASGATRRAVIEWPSRTLRRTRFVDTGRLEARDVYREADAVLYLTTHLDDSDLSFLWAGRGLRGPSVFPIHTIVVLSRADATSGGRADAMLTARQLARRRRREPRVGTLCQDVIAVSPLVGHAARTLREEEFRVLAELAAWPRASLEPHLLSVDRFTAPGALPGVTAEQRDQLVRRLGLGGLRLAITLTRTGCTTRAALAEKLQEYSGLKDLQSAVAELFTTRRAALKARSALTVLDQLLRTEPAPGLGEQLAEIELLCADLHPFRELRLLSALRCGRVDLAPETAADARRLLGGTGTSVGERLGMPDEASTDDIYAAAYAAAERWRQESHRPDTSAAQRRAAAVVLRSCDTITEWLAGTQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV05</accession>
    <name>U5VV05_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative type IV pilus biogenesis protein PilC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_05985</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSP F family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359017.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05985"/>
    <dbReference type="EnsemblBacteria" id="AGZ39486">
        <property type="protein sequence ID" value="AGZ39486"/>
        <property type="gene ID" value="AFR_05985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1216"/>
    <dbReference type="eggNOG" id="COG1459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035032_2_1_11"/>
    <dbReference type="OrthoDB" id="1268170at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003004">
        <property type="entry name" value="GspF/PilC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30012">
        <property type="entry name" value="PTHR30012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00812">
        <property type="entry name" value="BCTERIALGSPF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="8">
        <location>
            <begin position="73"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="8">
        <location>
            <begin position="277"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="410" mass="44689" checksum="20887A459381E82F" modified="2014-01-22" version="1">MPATKTFHYNSIDASGRKTKGTVEAANEIAATHLLRQRGEVPLEMSAAGQGLNKDLKIPGLGNRTKLKDLAVFSRQFSTMTASGMSLLRSLAILEEQTTAVTLKKATGEVRTDVAGGVSLSSAMAKHDKVFPRLMIAMIRAGEAGGMIDQALEQIAASLEKDTALRGKIKSALTYPAIVLSFTFVLIAAVLIFIVPIFEQMFKNLGGELPAITQFLVDASHNMWWIGPLVLTVGITGTVAYKRRIRESEDFRLSVDKLKLRMPVFGPLFRKLAMSRFSRNLGLLLNVGVPVMQALAVVGETTGNEVINRAMRDVQSAVRDGQPMSSAMRQHKVFPEMVTQMIEVGEESGQISQMLDKVADFYDREVDSAAESLTASIEPIMVLVMGAVVGGMVICLYLPMFTIYQNIQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV20</accession>
    <name>U5VV20_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12810"/>
    <dbReference type="EnsemblBacteria" id="AGZ40848">
        <property type="protein sequence ID" value="AGZ40848"/>
        <property type="gene ID" value="AFR_12810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2602"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_28_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="16831" checksum="937E7B7C49B8D308" modified="2014-01-22" version="1">MVDVAAHLFRGRGIAGVSVADVMAEAGLTPGGFYKQFASKDALIAEAVRAAFAEQEPTLAVPGDEASRRAFVDRYLSEEHRDHPDQGCPCAGFGGDLARGPHEARQAFTEGVEADARHLARDDEDGLADLCTLVGAIILARATGGTDLSGRILAAARSALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV71</accession>
    <name>U5VV71_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid adenylation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_12415</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12415"/>
    <dbReference type="EnsemblBacteria" id="AGZ40769">
        <property type="protein sequence ID" value="AGZ40769"/>
        <property type="gene ID" value="AFR_12415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2522"/>
    <dbReference type="eggNOG" id="COG1020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_222917_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensatn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010060">
        <property type="entry name" value="NRPS_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01720">
        <property type="entry name" value="NRPS-para261"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="1012"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="2512"/>
            <end position="2587"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="3565"/>
            <end position="3639"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="5062"/>
            <end position="5137"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="6108"/>
            <end position="6182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="5042"/>
            <end position="5061"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="1047"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="2547"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="3600"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="5097"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="6143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="6637" mass="711977" checksum="33551FF0E88F554D" modified="2014-01-22" version="1">[encoded data, 6637 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV87</accession>
    <name>U5VV87_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360982.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13185"/>
    <dbReference type="EnsemblBacteria" id="AGZ40923">
        <property type="protein sequence ID" value="AGZ40923"/>
        <property type="gene ID" value="AFR_13185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2677"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_4_11"/>
    <dbReference type="OrthoDB" id="1361074at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_3" evidence="1">
        <location>
            <begin position="80"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="33199" checksum="499F13ED658CB38B" modified="2014-01-22" version="1">MELAEAVAAFLATKPDSGDPGAPVAVRRTAIHHGSDQLFDMFGAAVAPVGVTDHVLDRPGGGIRVRVYRPATTRALPLYVFLHGGGFWLGSVEEKVNEALCHDRSGRAQCVVVAVDYRLAPEHPFPVPLEDCFAGLSWAVEHAAELGADPERVAIGGISAGATLAAAVTLLARTRGGPRIDLQLLEVPPLDLTLDTMVSSGIGDDYGITVDDMRLCADLYLGAGPDRRDPLVSPLHAADLTGLPPARIQSAEHDPLRLDGERYAQRLREAGVPVSFAVYPGAVHGSLALTGAWPPAATWHQDIVDALSRLRTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV89</accession>
    <name>U5VV89_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative patatin-like phospholipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_06630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06630"/>
    <dbReference type="EnsemblBacteria" id="AGZ39615">
        <property type="protein sequence ID" value="AGZ39615"/>
        <property type="gene ID" value="AFR_06630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1353"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055284_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="26"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="170"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="262" mass="27309" checksum="FE998AAC146B0D8D" modified="2014-01-22" version="1">MAWETGLIAGLAELGVDLSTADMFVGTSAGAIVTAQLSTGVPIAELFAGQTSAVIAEELTGSLGVGGILRFMVTGAWWGDHQRGRAWLGRAALRASTPPEAERRPIIEQRLPVHDWPSRPLRLTAVDAESGEFVTFDAGSGVPLVDAVAASCAVPTVWAPVTINGRRYVDGGVRSLANVDAAAGCDRLVVLSPRIAALRRGDRPDAQAATLGVPHVVISADRTAQATMGANPMNPAYRVPAARAGFEQAARVVDQVRGVWAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVI7</accession>
    <name>U5VVI7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_12680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 4">Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the UPF0677 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360881.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12680"/>
    <dbReference type="EnsemblBacteria" id="AGZ40822">
        <property type="protein sequence ID" value="AGZ40822"/>
        <property type="gene ID" value="AFR_12680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2575"/>
    <dbReference type="eggNOG" id="COG3315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056160_2_0_11"/>
    <dbReference type="OrthoDB" id="847145at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011610">
        <property type="entry name" value="CHP00027_methylltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00027">
        <property type="entry name" value="mthyl_TIGR00027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="32413" checksum="8F094F497E5BFDAD" modified="2014-01-22" version="1">MSENSPAIGSTPDTPIGAGSRPLPDGVEWTAVLVAAQRAAESLSQTPAFRDPLAEAVMTYLGLAEPGRAPHFATMPGQMSGMTEAMGDLVTLRTLYLDEALTRSGLDQVVLLGAGLDGRAYRMPWTGRRVFEVDRPAGLALKEQAADAAGLSAVARRTSVPVDLAEDWPQALVAAGFDPARPAAWVAEGLTVYLTRAEADLMMSRVHDLSAPGSRLCVEIAQPLGTRLDDAATSDAGVHRTLSVLRYGPPTPPHDWIAGHGWSPDASTLAELGDRYHRKVGTPFDPARGGVNLWYFNAELLPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVP7</accession>
    <name>U5VVP7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_13005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13005"/>
    <dbReference type="EnsemblBacteria" id="AGZ40887">
        <property type="protein sequence ID" value="AGZ40887"/>
        <property type="gene ID" value="AFR_13005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2641"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_317053_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665259" evidence="1">
        <location>
            <begin position="24"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="75"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase" evidence="2">
        <location>
            <begin position="531"/>
            <end position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="919" mass="98289" checksum="A69009332DAB1D44" modified="2014-01-22" version="1">MIRRAAAGGVVVALLLTLSPAPASAGPGSVRWTPCAAGFDCASVSVPLDHDRPRGEQITIALTRMRATDPQRRLGSLLVNPGGPGLSGVAFARTNFGDLPAELQARFDLVGFDSRGLAGSTALRCYESAEQAGADRAPFHYPETPAEENVWRQADDKMSAACTERGAPIVDHMSSAEVARDLERLRIALGDRKLTYLGYSYGSLLGQTYANLFPDNFRALVIDGVIDPVVATRGPAGAARTTPTASRMAAARGAGRTLAEFFRVCDAAGSACDFSGRSSARFARLADRLRAGPVDIVDPSTGATRTFRYNDLIGTALGAIFNAAGWPALARFLAGLESQLESTGPRVSIKAVPEEYPNEGEGKPGVACSDTVNPRSFAVWQDAADRAEREYGYFARIYNWVWSQCRSWPASAGQDSYHGPWTGTHGGDGADRRQLLRPGHPVRGCRGRGPAAPQFAAAVVRGMGSCCLSPAGQRLRGHTGEPLPADREATGAGHGVPTGPALPALTGAVSGVEVEEDPAALGFVGDRMAAIDRHFRRYVDDGLLPGWQIVVTRHGRVAHSSVYGQRDLAAGTPVEADTLWRIFSMTKPITSVAAMTLWEEGRFQLTDEISRWLPAFADMRVYDRGSALKPYTVPAAEPIRVWHLLSHTAGLTYGFMHTSVVDQLYRNAGLDVTDPALDLATTVDRYAQMPLLFQPGTAWGYSVATDVLARLVEVVAGQDFDTVLTERVLTPLKMTGVRRWVTAPDATRLATLYAADPTTGRPVPAEEPAWQALRPPELRAGGHGLICTAADYHRFTQMLLNGGELDGRRVLGSRTVRLMTRNHLPGGQDLAALSTGGFAETSLEGVGFGLGFAVTQDPVPGRSSNNAGEFSWGGLASTTFWVDPVDRVTALFFTQLMPSSTYPLRQQLGQLVYSALAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWF2</accession>
    <name>U5VWF2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_15255</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361392.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15255"/>
    <dbReference type="EnsemblBacteria" id="AGZ41333">
        <property type="protein sequence ID" value="AGZ41333"/>
        <property type="gene ID" value="AFR_15255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3097"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="19"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="103"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="176" mass="19656" checksum="4CF3BD46827078AB" modified="2014-01-22" version="1">MRPEQEREYIEYVSGRLHSLHRTAYMLCGDAHRADDIVQATLTSLYVQWKRVTAADNVDAYVHTILVRRYLDERRLRWSRVRLSDAPVQAATADSADHGLAEREELITALRALPKGQRAVVVLRYFGDLSVEATAEALKCSVGNVKSQCARGLATLRAALHQEGHVGTVQGMRDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWJ7</accession>
    <name>U5VWJ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08905</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359601.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08905"/>
    <dbReference type="EnsemblBacteria" id="AGZ40070">
        <property type="protein sequence ID" value="AGZ40070"/>
        <property type="gene ID" value="AFR_08905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1813"/>
    <dbReference type="eggNOG" id="COG3325">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002833_14_4_11"/>
    <dbReference type="OrthoDB" id="1081028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="SSF54556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665281" evidence="5">
        <location>
            <begin position="29"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="6">
        <location>
            <begin position="34"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="429" mass="46457" checksum="84CF9D6312138229" modified="2014-01-22" version="1">MRGFSRRLLAVGAAVLVGVTAAPTAAQAHPKKSYVKVGYFTQWGIYGRDFQLAKVQQSGAAAGLTHLNYAFGPVTAAGVCDSADSWADWGVPFSDAQSVDGVADVAGQPYSGNLNQLAELKKKNPKLRVLISLGGWTGSAFFSDAALTDASRKKLVTSCVDLWIKGNLPGLTTGVAAGVFDGIDLDWEWPGSDGNVGNVIRPEDKQNFTLLTAEFRKQLDALSRKTRKHYDLTAFLPAAPAKIDAGFEAKKIFKYLDFGTVQGYDFHGSWEARTNQQSALRVPAGAPDNPDFSVEVAINTWKDRGAPKNKLVLGIPYYGQGWTGTTGDLFAPATGGAPGKFAVGTEDYKVLKNLKSQGFKLHRDWRSGHSWLTDGTTFWTYDDPAQILQKTTYIRSQGLGGAMMWSLDGDDEKASLTRTISAGLWTPWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWM8</accession>
    <name>U5VWM8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pentapeptide repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40110">
        <property type="protein sequence ID" value="AGZ40110"/>
        <property type="gene ID" value="AFR_09105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1852"/>
    <dbReference type="eggNOG" id="COG1357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033401_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="22855" checksum="1B19E7E2E5A5C8EF" modified="2014-01-22" version="1">MTPAYFRTATLDLHEFEPEAAYEGLAFTGLDLSGRESESLEIAQCRFRSVDLSGSLLPHLSLTDCQVQTSNWANVRSDGGAVHRAIFEESRMTGFTVTDGKLSDVAFDQCRLDLSGWRFTAFDSVRFTGCNLAGADFTNADLRGARFSNCDLTGAQFHHADMDGTRFRRCELDGIAGITSWRGVIVHADDLPALSYTLAAGLGIKVDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX04</accession>
    <name>U5VX04_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Patatin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_15200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361381.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15200"/>
    <dbReference type="EnsemblBacteria" id="AGZ41322">
        <property type="protein sequence ID" value="AGZ41322"/>
        <property type="gene ID" value="AFR_15200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3086"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055284_1_0_11"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="6"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="28627" checksum="1380D844CE892F2A" modified="2014-01-22" version="1">METALVLGAGGITGIAWHLGLITGLREAGVDLTDAGLVVGTSAGSVTGTLITAGVDPVDAQRLEARLGASDPPIRPDWARGAQAYALLNDEERDPAAIRAAVGALAVSANVIAEEPYVESLARRLPVQQWPERRLLITAVDAGSGEPVVWDRSSGVSLRRAVAASCAVPCIFPAVTIDGRPYMDGGVRSGTNADLAAEARRVVVLAPLAPVRMRGAPAAEIDGLRQRSKVALVAPDDLALAALGPNPFDATRWEPAIEAAIAQGHRVAPEVAAVWLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX40</accession>
    <name>U5VX40_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_15785</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361498.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41439">
        <property type="protein sequence ID" value="AGZ41439"/>
        <property type="gene ID" value="AFR_15785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3204"/>
    <dbReference type="HOGENOM" id="CLU_822912_0_0_11"/>
    <dbReference type="OrthoDB" id="2155932at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665305" evidence="3">
        <location>
            <begin position="30"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="337" mass="35131" checksum="337C9983A90666E8" modified="2014-01-22" version="1">MTARLARSFAILVLVLGTAIGFGPAPAWAAPIDLKMTLTGPDEVTGGAVVAYHLRVDAGPAADAASVKASLYLPSGVIFSPDDWQDQDESGPCVAGLCTVTRPEHVERGYFTWTVRLVFDRSMSPDTRLGLRATAASAGEEATPADNVADLDVTFLGLSDVALTAVPPNGPVTAGEPVRFAVIAKNVGDTRISRLTFTYAELGLWFTGGDIDVDGAECVADPGEMVCDFAVDLERGESRRIEHTFPTHANEDTWGANGVVRIRARDGDPANSDIRVKFVFAPKPGTTTPPTTPPTAAPGGGGGLPVTGSATLPLGLGGLVLLLAGTAALWVTRRRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX80</accession>
    <name>U5VX80_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10230"/>
    <dbReference type="EnsemblBacteria" id="AGZ40335">
        <property type="protein sequence ID" value="AGZ40335"/>
        <property type="gene ID" value="AFR_10230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10230"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="2">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="467" mass="49740" checksum="CC7FD7D926ED4114" modified="2014-01-22" version="1">MGGVDLVISLDGTGEKTVGVYRALRAAIVDGRLPVGHRLPATRVLAADLGVARNSVATAYERLVAEGYLVSRVGAGTYVAPVRDAPAPRRVAADPLRPRAGWTFTPQPTSGDTPAPRYDFRSGVPDARLFPFDSWRRLVTAELRLRANNPGAYATPAGHPALRAAIARYVGYSRSVRASEDDVLVTNGTQHALDLIGRVLLRPGDVVAVEEPGYPPARRLFESLGARVVGVPVDEHGLVVDDLPGTARVVYATPSHQFPLGRAMSLGRRHALLDWARRRQVAIVEDDYDSEFRFSARPLEPLHSLDTAGRVLYVGTFSKSMVPGVRTGFVLAPAGLREALIAARQLGDGYGQVAVQAALARFIDEGQLARHVRRAGKEYAERHARIVAALSAHDRLQVIPSAAGLHVTALGANSAAVVAAAARRGVELDDLGAYGSDSTGFVFGFGAIDPALLDEGLAIFGAVLRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXB3</accession>
    <name>U5VXB3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361491.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15750"/>
    <dbReference type="EnsemblBacteria" id="AGZ41432">
        <property type="protein sequence ID" value="AGZ41432"/>
        <property type="gene ID" value="AFR_15750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3198"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="25353" checksum="F18FAA8E2A7EF56A" modified="2014-01-22" version="1">MGPLAGKVVLVVGASAGIGADTARVLAADGAALMLVARSAGPLAELTAELREAGHEVAYTTGDVSRAVDVAAFVEATVARFGRLDGAFNSAAMTQGGRLDEVPEDDIDRILAVNVKGTWLCLREQARVMEKQGSGSIVTVSSIGGLRGSSGMGAYQATKHAVIGLTRTAAHDFGPLGIRVNCIAPGPTESPMLDQTRLAIPGGVEKRIAATPLRKAGTGAEVGAVASFLLSDRASHLSGVVLPVDGGFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXJ2</accession>
    <name>U5VXJ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361851.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17050"/>
    <dbReference type="EnsemblBacteria" id="AGZ41688">
        <property type="protein sequence ID" value="AGZ41688"/>
        <property type="gene ID" value="AFR_17050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3459"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107158_0_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="20492" checksum="CB9598BF5C94CEAC" modified="2014-01-22" version="1">MPRGVAIPELRQQLFAAVERVIAGDGPGRLTGRAVTREAGVATGLLHAHFTTFEDFLTGYAVDRAFLISGEVAGLPARAGTGSVAGNVADALLAAPPATLLSLTRLLAYRPELTAGIEAVLGDGSAGLQAAERAIAAYLAAERGLGRLPDNTDTESLALAAVAVLHHVALTGESGASAEARIRRATAALTGTTRLPEEHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXK9</accession>
    <name>U5VXK9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Periplasmic binding protein/LacI transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361891.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17150"/>
    <dbReference type="EnsemblBacteria" id="AGZ41708">
        <property type="protein sequence ID" value="AGZ41708"/>
        <property type="gene ID" value="AFR_17150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3479"/>
    <dbReference type="eggNOG" id="COG1879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_3_3_11"/>
    <dbReference type="OrthoDB" id="1378040at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666181" evidence="1">
        <location>
            <begin position="26"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_4" evidence="2">
        <location>
            <begin position="47"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="34899" checksum="64528731A5631464" modified="2014-01-22" version="1">MNVSRTVRHRVVASGLAAGLLVSAAACGSDDAGSGSGSGSDSTDVTIGIVELNLSNPFFGTLEKATEAAAKAKGWKVMKAEAKVPGDSATQVTAIENMIANKVKAIVLDPANPTALVDVVKKARDKGILVVTVNATLTPIEAADATFATDNTAAGKLIGQWAKATAPASPRIAMLDFDLSDGPAGGRHNGYLEGYGITGEDPKIAGSALTKGTVETGQSAMENLLSAHADINTVYTINEPVAHGAYTAIKNKGLEGKVSITSVDGACSGVQDVKDGIIGATAMQFPSKMGELAVAAADAFLKDGTKPKSGLNDSGTVLITDKPVDGIESQTSEWGLQNCWGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXN8</accession>
    <name>U5VXN8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coproporphyrin III ferrochelatase</fullName>
            <ecNumber evidence="8">4.99.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hemH</name>
        <name evidence="8" type="synonym">cpfC</name>
        <name evidence="10" type="ORF">AFR_11055</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in coproporphyrin-dependent heme b biosynthesis. Catalyzes the insertion of ferrous iron into coproporphyrin III to form Fe-coproporphyrin III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Fe-coproporphyrin III + 2 H(+) = coproporphyrin III + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:49572"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:68438"/>
            <dbReference type="ChEBI" id="CHEBI:131725"/>
            <dbReference type="EC" id="4.99.1.9"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="7">
            <dbReference type="Rhea" id="RHEA:49574"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8 9">Porphyrin-containing compound metabolism; protoheme biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the ferrochelatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.9" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360430.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11055"/>
    <dbReference type="EnsemblBacteria" id="AGZ40500">
        <property type="protein sequence ID" value="AGZ40500"/>
        <property type="gene ID" value="AFR_11055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2252"/>
    <dbReference type="eggNOG" id="COG0276">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018884_2_0_11"/>
    <dbReference type="OrthoDB" id="780534at2"/>
    <dbReference type="UniPathway" id="UPA00252"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="PTHR11108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8 9" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="3 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Fe-coproporphyrin III</name>
            <dbReference type="ChEBI" id="CHEBI:68438"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Fe-coproporphyrin III</name>
            <dbReference type="ChEBI" id="CHEBI:68438"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="342" mass="37178" checksum="F686980D80097E7F" modified="2014-01-22" version="1">MAYDAFLLLSFGGPEHPDDVMPFLRNVTRGRGIPEERLTEVAEHYYHFDGVSPINQQCRDLLAAVQAEFTAHDIALPAYWGNRNWTPMLADTMAQMRDDGITSALGFATSAYGGYSSCKQYWEDIAQARAKVGPGAPAVAKLRQFHDHPGFVTPHAEAVTAALATLDPQRRASTRLVFTAHSIPSSMAATAGPDGGRYEAQLHETAQLVHAAAAPDLPWDLVWQSRSGPPQVPWLEPDINDHLTALAEKDVTDVVVSPIGFVSDHLEVLWDLDNEARDTAAGLGLGYARAATPGTHPGFVTMVRELVEERTAAVPRERLGTLPIWDQCPANCCPPPARRGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXS2</accession>
    <name>U5VXS2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360366.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10885"/>
    <dbReference type="EnsemblBacteria" id="AGZ40466">
        <property type="protein sequence ID" value="AGZ40466"/>
        <property type="gene ID" value="AFR_10885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2217"/>
    <dbReference type="eggNOG" id="COG3220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064263_0_0_11"/>
    <dbReference type="OrthoDB" id="1602134at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007801">
        <property type="entry name" value="UPF0276"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42194">
        <property type="entry name" value="PTHR42194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05114">
        <property type="entry name" value="DUF692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="272" mass="28926" checksum="2BA35F4C31563FC5" modified="2014-01-22" version="1">MTTAYGVGIGWRPEIAGFVAQLPGLKFAEVVAESIHEHSDLPTGLTDLLDRGVAIVPHGVKLSLGGAEPVEPARVTHLAAVAERLGAPLVSEHIAFVRAGGVEAGHLLPIPRSREAVDAVVANVKRTQAELGVPIALEPIAALFDWPDDDLDEGAFLTEILERTDALLLLDVANVYANALNRGTDPAALFDRLPLDRIAYTHIAGGAEHDGLYHDTHTDAVPQPVLDLVTALCDRHRPPALMLERDGDYPPAAELRSELDAIAKASGYPTVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYH9</accession>
    <name>U5VYH9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membrane-bound lytic murein transglycosylase F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_17795</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362147.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17795"/>
    <dbReference type="EnsemblBacteria" id="AGZ41837">
        <property type="protein sequence ID" value="AGZ41837"/>
        <property type="gene ID" value="AFR_17795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3609"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_18_1_11"/>
    <dbReference type="OrthoDB" id="847343at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665875" evidence="2">
        <location>
            <begin position="31"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="62"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="32355" checksum="34AF07A1BAC7FA7F" modified="2014-01-22" version="1">MKIHTPTRLRYAVAGVVLTLATAGCGTASADETTGDGTATGAVAFSQQIHDRLPDAVQKSGVIRLATDPSYAPMESYAADGRTIIGFEPDLAAALGAVAGVRVEMVPAEFSSMIDGTRKGTFDGVLSSMNDTPEREKKVDFIDYFSAGLAIVVQRGNPQGITDLKDLCGQVVAAEKATVQVDLLRRTQPACGDRPIVIKTFKTNADALLQVRTGRAAAILNDYPPAAYLATDPRTRSRYQLASTVQYEPGLFGIAVAKDNPELRDALRDALDQLIRSGTYTELLERWGLASGAVTTSSVNSGAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYU4</accession>
    <name>U5VYU4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Subtilin biosynthesis protein spaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18395</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362330.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18395"/>
    <dbReference type="EnsemblBacteria" id="AGZ41957">
        <property type="protein sequence ID" value="AGZ41957"/>
        <property type="gene ID" value="AFR_18395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3733"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010573_0_0_11"/>
    <dbReference type="OrthoDB" id="1149848at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006827">
        <property type="entry name" value="Lant_deHydtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023809">
        <property type="entry name" value="Thiopep_bacteriocin_synth_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14028">
        <property type="entry name" value="Lant_dehydr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04738">
        <property type="entry name" value="Lant_dehydr_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03891">
        <property type="entry name" value="thiopep_ocin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lant_dehydr_N" evidence="1">
        <location>
            <begin position="49"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Lant_dehydr_N" evidence="1">
        <location>
            <begin position="235"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Lant_dehydr_C" evidence="2">
        <location>
            <begin position="632"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="874" mass="95009" checksum="2CEC03F95D89381A" modified="2014-01-22" version="1">MPSPPLPPEPGAWRFLPLIMVRMPALSLAAFSRTTTVDESDPVGALWRCPELRDAVRLANPQLAAAADRSRTGTSDRRERLAAALTKYTIRMATRPTPFGLFAMTGFSTWGDGPLGPPATPPVRRRRIDSRRAAGLLREAARGRPVAANPTARVLADRITFQPPYATRRRTLRRSRRIDTLLAFTASPQPWAAVATLLGDETVASRLLDEGLLVSTLHPTAPEGFPPHWPADGRADLTMHPTGTVDRRLAEGLSAALAVLQRAGLATEADPGLRHYAAAFAERYGFEEVPLEDVADPASGLGPLPPLGASEDAPALDGLRRLLVRRGPVAVLSASDLADLPALHRGAAPSCDLFVQLLPDGRAAVTPRGAVVPGGRAFARFADADDRVPEHVRTAAHAPGEEDIDLVAMDYWHESTINLGEGGSAYDTVLLWSSVRPGRRALRVKDLLVGVQDGALYLRSRLDGRRVLVRTDHLVATETAPALLDLVHRISRAHVATPGWSWGRFADAHSALPRVETAGVVLSAARWRLPDDKITAWREDLQVPRFVYAGTGDTRLLLDLDHPRHRALLREQRRRGAEWAEECLFDPDGTGHVTEFVVTAVNDAYRPLPPLRTARPGDFTGTTPARLPGDGWWSTHVTCAPGSQDEVLGLLATYLAGQDARWFFTRYDNPAPHLRVRVHSEVLDVAQWCAALRLIARRGPVLRFTLEPYEPETRRYGGPAGLALCEQLFTVDTLVVLDAQHPADTDPSTLAAVLIDLFATMVGAAPATLERAVKRLAGPPPEGDRGARAEEHRQRRILVDLLTAGPGKELAGAYDSRPIDPALTDLINGEHGEAVLAGLLHMHLNRHGLPPTAEPAGARRRLAVQHSLNHRPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYY5</accession>
    <name>U5VYY5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4173 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_13295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361004.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13295"/>
    <dbReference type="EnsemblBacteria" id="AGZ40945">
        <property type="protein sequence ID" value="AGZ40945"/>
        <property type="gene ID" value="AFR_13295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2699"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025121_0_0_11"/>
    <dbReference type="OrthoDB" id="1213947at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025231">
        <property type="entry name" value="DUF4173"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13777">
        <property type="entry name" value="DUF4173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="542"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="580"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4173" evidence="3">
        <location>
            <begin position="473"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="728" mass="76518" checksum="9EF816A55075051C" modified="2014-01-22" version="1">MADLPPKQPDPPETAKAVPADPPPAPQLLVMPPTDAIPSLVWPGPEGQPAWAIPVQISPGTRGYALFVPMVPVEAPAAPAVAEPADRPVKPSPKPAATTPTVTAPAAPTPAEVTTPAVTAPAAPTPAAVTTPAATTPATTSPTATSPATTSPTATSPVAATPAAATPAAATPAAATPAAATPAAAKRVVPPPPDLPPAKAQPPAPQRPPMQSPPFPPRPMGPYDRPPSAWRTFREKHWPGPKDAQGWAVPAGVLAGALGFLIFLPLNRVGIGWFLGWLALTLGVGFAVRKSADLPRRERLLRSGWAVAALALLLIPAFRNAWWLVTFSVLGAIGCTALAIVGGRQIRSILFSLVAAPYAAFKGIPWVRRHLKAGRNPGIAGRIFFSVALTAVVLLVFGALLSSADVAFSQLLDDAIPDLSISSIFKWIFLAVAGALLAVSGVYTLTAPPATSSLDTEGKGRFGLIEWAPAGAALVLLFCGFVAVQFTVLFGGSRHVLETAGLSYSQYARSGFWQLVAVTLLSLALIASLARWAKRDTKAERLLLRVLLGLLCALSVIIVASALSRMWEYQRVYSFTGERIFVMASEMLLGVIFVLIAVAGIKWQGRWIPGATVGLAVLMLLGLAALDPEGYVASRNAARYEGSGKIDAWYLRALSADATPALTELPDPVRRCTLSWIAEDLKEPDPWYAWNLGRQRAREALADLGPAAIGSQKDCRAADQFDLPKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ32</accession>
    <name>U5VZ32_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362486.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19210"/>
    <dbReference type="EnsemblBacteria" id="AGZ42114">
        <property type="protein sequence ID" value="AGZ42114"/>
        <property type="gene ID" value="AFR_19210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3902"/>
    <dbReference type="HOGENOM" id="CLU_971925_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="33324" checksum="22E8766E8EBF198A" modified="2014-01-22" version="1">MIFGQSYMGGSAVQAAEHDAERKQSTEDCSPTEIPHPGGLLQRLEQRPGLSGMVALTTFAALGVGVLAAVPDWRDMVMNGDPPPADVHTAEVSVTPTPSSAATPDLRVLDLVLRDPLRSGGTVGGEPEFTLPSAEFTVHNPGTHRAVITRVIMKIEDSAVIEQCAAEGMPVAVSAEYDVLMPLRPADGAELQVPVSQHQASDEADRFTLRFGSPEPLSPTTVHLYRIRFELALGRDGARLDAGTAVVATPLTPVDGAGYFWSADHDSGRVVFGPDFTGREEVIACMKSNSGAMTSLLSGAGGLSPALARSLSDLRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ40</accession>
    <name>U5VZ40_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoadipate enol-lactonase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362496.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19260"/>
    <dbReference type="EnsemblBacteria" id="AGZ42124">
        <property type="protein sequence ID" value="AGZ42124"/>
        <property type="gene ID" value="AFR_19260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3912"/>
    <dbReference type="eggNOG" id="COG0599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070025_3_1_11"/>
    <dbReference type="OrthoDB" id="1792151at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012788">
        <property type="entry name" value="Decarb_PcaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02425">
        <property type="entry name" value="decarb_PcaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="34"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="13603" checksum="B786BCFEF030DFEC" modified="2014-01-22" version="1">MGDSYEDGMTVRREVLGDAHVDRAVAATTAFTADFQDFITRYAWGGVWTRDGLDRRTRSCVTLAVLTALHCHEELAMHVRAARRNGLSADEIGEVLLHTGVYAGVPAANAAFKIAQETLAADEPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZJ9</accession>
    <name>U5VZJ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">OmpA/MotB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_20755</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20755"/>
    <dbReference type="EnsemblBacteria" id="AGZ42423">
        <property type="protein sequence ID" value="AGZ42423"/>
        <property type="gene ID" value="AFR_20755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4208"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_611974_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpA-like" evidence="4">
        <location>
            <begin position="166"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="257"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="447" mass="46892" checksum="19CF4C2AF4D886FA" modified="2014-01-22" version="1">MSVGPLIRSGGTVVLTVQTTLEQAGNGGAAVVSRHFSSILSTSFDAARLIDEAGRRVYLVSRNTADDGCVCTGLLRVEAGETRPLQAAFGGLPAGLQRVSVMLPHAGVFTDVPILDGAVPEPPTGVNAAGRVQKALDLSDVAASYGAGLEAYTERVGVGVRTRRGADQVDISLDTDVLFRLDSAKLTSRASKTIGAAVADLKAAGPGPLTVTGHTDSTGTTAHNQTLSEQRARVVSEALTTQLPDGQWPKTVAAKGESQPTVPNDSDANRALNRRVTITFKERRNNTGAGSTAPLPKTKGEEGKAPDGVEVSLPLNRGTIRFVPGAATVRGPFLLVNLTARNTGEGKATILDFLGQGVFTVRSEFDPFARYGAAGVRLLQGDTASYGLDYELEPGDHRCLCDRLLNQAIPPGSERLLSLWFPAPPAGEKVITIDVADRLRITDIPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W000</accession>
    <name>U5W000_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leu/Ile/Val-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_20400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leucine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362724.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20400"/>
    <dbReference type="EnsemblBacteria" id="AGZ42352">
        <property type="protein sequence ID" value="AGZ42352"/>
        <property type="gene ID" value="AFR_20400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4136"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000709">
        <property type="entry name" value="Leu_Ile_Val-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00337">
        <property type="entry name" value="LEUILEVALBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665398" evidence="3">
        <location>
            <begin position="26"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="4">
        <location>
            <begin position="39"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="385" mass="39667" checksum="DC0A39653566D8AD" modified="2014-01-22" version="1">MRSRHLLSSAAVAVVLAALAGCGGADDPATAPAAAPDVIRLGTLVPLTGRSSPSGEAMVKAAQLAVSEANKAGGVLGRQIELVVGDDACDPGTAVTAARTLVGKDIAVSVGGYCSSATVPTLKIFRDAQVPMIVAQSNSTDLLSPGYDSVFLVCGTVTAEADFAVDWFKKLGGKKLAVVHDGTSFPITLAQSTVTAAKRVGGLTVTDELELSQGAPSYTRIAASVIAGKADTVYFTGYYAEANQLIKDLRDEGFRGRIAVGDGATDGPLLANLTPAQSKDVYGTALLFPEFMPELADWSARYRAANGTAPGPSTVEAYDAVNVALDAIKRAGSLDHEAIRKAISTTDMTSMSGPMSFHPDGTRVTPKFLLLRAAGDTFELEPTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W021</accession>
    <name>U5W021_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type spermidine/putrescine transport system, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_20525</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362749.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20525"/>
    <dbReference type="EnsemblBacteria" id="AGZ42377">
        <property type="protein sequence ID" value="AGZ42377"/>
        <property type="gene ID" value="AFR_20525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4162"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_11"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="353" mass="38248" checksum="E267CE9EF4642BEA" modified="2014-01-22" version="1">MSFEELRLDGVSRSFGGHAALAGLDLTIKRGEFIALLGPSGCGKSTALNCLAGLLPLTRGSIWQDEARLDVLPPERRGFGMVFQNYALFPHLPVRKNIAFGLQMRRVPRAEIRRRVDEALRLVHLEDQAGKLPGQLSGGQQQRVAIARAVVLEPSLVLMDEPLSNLDAKLRLEMRTEIRRLHQSLGLTTVYVTHDQEEALSLADRLVVLRAGRVQQIGTPEQLHTEPANWHVADFMGYRNLLPLKAGARRGAGVVVEVEGRELLGTPVGDLAPGSDVIAGTRPEDLTIGPAGGTGVPATVEVVEYQGREVAVEARTPGGIPLNLRSVDRPAVGDRITVTVEPRRLLVFPADGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0F7</accession>
    <name>U5W0F7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361475.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15670"/>
    <dbReference type="EnsemblBacteria" id="AGZ41416">
        <property type="protein sequence ID" value="AGZ41416"/>
        <property type="gene ID" value="AFR_15670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3182"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_3_3_11"/>
    <dbReference type="OrthoDB" id="1815576at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="39"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="29142" checksum="E5126804DAF098BB" modified="2014-01-22" version="1">MTARYQVTIVKYGTRQTVRSDVYLNHHLYGEPDGPIGMDYFFWVVRNTERTIVVDTGFSAIGGANRKRTTLIEPAAAFDALGVDRAAGPPVVITHAHYDHIGNLGLFPASPLTVAGAEYAFWTGPYADRTLLHHSVEDEEIASLKQADAEGRLRPFDDEADLAPGVRVIRLGGHTPGQSVVTVETTDGVVLLASDSVHYYEEFERSMPFTQVSGLVDMYAAFERIRTMLATGEVRHLVPGHDPDTLDRFTPVTGPLAGLAATIGEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0G4</accession>
    <name>U5W0G4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_15965</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361534.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15965"/>
    <dbReference type="EnsemblBacteria" id="AGZ41475">
        <property type="protein sequence ID" value="AGZ41475"/>
        <property type="gene ID" value="AFR_15965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3240"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_985616_0_0_11"/>
    <dbReference type="OrthoDB" id="1891092at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="9">
        <location>
            <begin position="46"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ41475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="30216" checksum="E8E63D83E022A93C" modified="2014-01-22" version="1">MTLAYPTTIRHRSRTRKVYETLEAYIRLDLVEERMFPATSIMRYIAVVFPVLLYFFQSKFLGAGADVFGVMLIGTAVTVGLQDALTQLTARLNFAMERGTLETYLVEPVPWVLIPLAMNLWRSFTGGLVACFMIAVGWLLGMPIDPAGIPLGLAVLFLGILACNALGTLAASFLLLFKRGDPVVMLFSLAAGVLGGALFPVSVLPDWIRWVSYLVPHTYVISAERQLLMPNPPAGELGVGLSLVVLAVLCVVMLTAGLWIFDRSLRLARRLGILSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Q5</accession>
    <name>U5W0Q5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16245"/>
    <dbReference type="EnsemblBacteria" id="AGZ41531">
        <property type="protein sequence ID" value="AGZ41531"/>
        <property type="gene ID" value="AFR_16245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3296"/>
    <dbReference type="eggNOG" id="ENOG50320SJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1425217_0_0_11"/>
    <dbReference type="OrthoDB" id="2012099at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="190" mass="19831" checksum="E4BB0967903647FC" modified="2014-01-22" version="1">MPGSPLDRLLTAFLIVGPVTYLLADVLYALRGWDDPTAAVVHVLGAVAYAFTLLRVVTWTEGVQAAILLVVSVLGVAGNVAYGFNTIHVSLGDTDLVDATGAAVLIKPLGLFFPLALLLAAVALRRDRPVWVVATLAVAAVVWPVAHIANIAWLAVLVNVALIVAFRALAVPAGRHELLPQDAAGRGGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1L3</accession>
    <name>U5W1L3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363436.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23210"/>
    <dbReference type="EnsemblBacteria" id="AGZ42912">
        <property type="protein sequence ID" value="AGZ42912"/>
        <property type="gene ID" value="AFR_23210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4704"/>
    <dbReference type="HOGENOM" id="CLU_1275439_0_0_11"/>
    <dbReference type="OrthoDB" id="2129783at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="216" mass="23439" checksum="73A6FC7D02783E03" modified="2014-01-22" version="1">MSQANSSVTVEQAAQMRGFLEDVEGLARSVSDGVWPRLGQIEGTDATGTVYFSVDDDGAFASVYLDPHWWTALGVSGLPGALLGAQRIARDKLTAARLIFRRVIGPPRRRGPADLGEPGFELMHGLSASRDIGELLGRWHALTAESYQRMYEFDRRQADGSPLRVQGPEELVTFTLDGQVITGVEVDGWRLNAGSTDRLTADIRAALHHNTGGQHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1P4</accession>
    <name>U5W1P4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chaplin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_17815</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362155.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17815"/>
    <dbReference type="EnsemblBacteria" id="AGZ41841">
        <property type="protein sequence ID" value="AGZ41841"/>
        <property type="gene ID" value="AFR_17815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3613"/>
    <dbReference type="HOGENOM" id="CLU_2476369_0_0_11"/>
    <dbReference type="OrthoDB" id="2037979at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005528">
        <property type="entry name" value="ChpA-H"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03777">
        <property type="entry name" value="ChpA-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51884">
        <property type="entry name" value="CHAPLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0034">Amyloid</keyword>
    <keyword evidence="1" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665596" description="Chaplin domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Chaplin" evidence="4">
        <location>
            <begin position="48"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="87" mass="8825" checksum="C8BFAB77D3CEB282" modified="2014-01-22" version="1">MKLVRGTLVMLGTAAAALAFAAGPASAHYDDDDNYGGYGGGSQSSHHNVGLLNGNQAYFPISVPLNFCGNSIALLGVSQSSAACYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1P5</accession>
    <name>U5W1P5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_18135</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18135"/>
    <dbReference type="EnsemblBacteria" id="AGZ41905">
        <property type="protein sequence ID" value="AGZ41905"/>
        <property type="gene ID" value="AFR_18135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3681"/>
    <dbReference type="eggNOG" id="COG4934">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012501_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666283" evidence="4">
        <location>
            <begin position="34"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="5">
        <location>
            <begin position="238"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="651" mass="68601" checksum="DC261C63B83BDC7C" modified="2014-01-22" version="1">MQILSTTRVRLTAAVAVGAALVSVLGAAPPAAARPGRHTINGSKPRWLGRAKKAGTPAAAGTINFGLLLTMRDRAGAEAALAAISDPAAADYGKWLSAGQFEARYAPAAADVRAVRTWLKDQGFTLRGTLAGGMYVETSGTTAQINKTFGTTVENYTYQGHTVHANSTELSLPENTPAAVTGLVTGVIGVDQGSSLKTPGETLPPPGPGFRAGTPCSSYYGEKKATTLPTKAPWVVCGYEPKQYQSAYGVSDQLKRGLDGRGVTVAITDAYASPTITQDARQYNRKHGLPAFKNRQFRQITPPADGYANTELCDAQGWYGEETLDVEAVHAMAPGANVVYVGGSDCVTGLDEAWAETIDNHVADIITNSWGNATDDIELLGEDVVAFYQQFSLEAALTGITVDFSSGDSGDQTSGGTDVASKTVDFPADLPYVTGVGGTSVGIDKHSKRVWEHGWQSAYQTLENGVWGPSAYVSGGGGGTSVIFAQPFYQRGKVPAAISKYFGSTPARAVPDISMPGDPNTGFRVGQTQEFPDGTYYDEYRIGGTSLASPLLAGVQAIANQKAHHALGFVNPLYYSLLGTSALTDIRAPRTPIYQARANYVNSLDPSEGRTYILQQVDVQTSTIHSTPGYDAETGVGSPGPRFFAAMPGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2C5</accession>
    <name>U5W2C5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alkaline phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362424.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18885"/>
    <dbReference type="EnsemblBacteria" id="AGZ42051">
        <property type="protein sequence ID" value="AGZ42051"/>
        <property type="gene ID" value="AFR_18885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3836"/>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590278_0_0_11"/>
    <dbReference type="OrthoDB" id="516568at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039331">
        <property type="entry name" value="PPA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953">
        <property type="entry name" value="PTHR22953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666306" evidence="1">
        <location>
            <begin position="33"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="213"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="46571" checksum="BE035F87FBC48566" modified="2014-01-22" version="1">MGIRKFGPAAATALLTAATATVVLTSGTPASAATATFTPTADTYVQSDTAATGYGTATQIVVDNSPVRRSFLRFTVSGVSGTVTAAKLRLRTISGNDGSPAGGTFRSMTSTTWSETGTTWNNQPAIDGTAVGAIGAVAADGWYELDVTSVVSGNGSFSFGATSANSDGAYYDSRESGADAPQLVLTTGTTPPSDDPVLVGAGDIANSGSGDTATAALLDGIAGTVYTTGDNVYNNGTAAEFTSYYAPTWGRHKARTKPSPGNHDYNTSGATGYYNYFGVASYYSYELGNWHVVALNSNIAMAAGSAQERWLRTDLAASAKPCTLAYWHHPLFTSSANHAPSTSTRPLYQALYDYNADVAVWGHNHVYERFAPMNANGAADPARGLRSFVAGMGGASHYTFGTIQPGSEARNADAYGLLKFTLHANSYDWQFVPVAGKTYSDTGTGACH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2G6</accession>
    <name>U5W2G6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364120.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25820"/>
    <dbReference type="EnsemblBacteria" id="AGZ43428">
        <property type="protein sequence ID" value="AGZ43428"/>
        <property type="gene ID" value="AFR_25820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5234"/>
    <dbReference type="eggNOG" id="ENOG5033FXZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465261_0_0_11"/>
    <dbReference type="OrthoDB" id="1808149at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="184" mass="20814" checksum="6940F764135ED7FE" modified="2014-01-22" version="1">MIDVEKPVWLLDVDGVINTTRPGWGGSPQRSLVWSGADNTSYLMRWAPGLIDRIRALVLEDKVEIRWCTTWCLEADRLERLWSLPAFARTLDNDPMPRGPDCWPLKLAAAWSVLAEGRRLIWTDDEALPPPGSDRDELTVDGRALLIAPRSNRGLQPEDLDLIAKFAEGRRLPTRRVAGRHRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2R2</accession>
    <name>U5W2R2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA polymerase factor sigma-70</fullName>
            <ecNumber evidence="9">2.7.7.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_26280</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26280"/>
    <dbReference type="EnsemblBacteria" id="AGZ43518">
        <property type="protein sequence ID" value="AGZ43518"/>
        <property type="gene ID" value="AFR_26280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5326"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043648_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014305">
        <property type="entry name" value="RNA_pol_sigma-G_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02960">
        <property type="entry name" value="SigX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="124"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="SnoaL-like" evidence="8">
        <location>
            <begin position="194"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="34650" checksum="E21756CC1F5E119F" modified="2014-01-22" version="1">MQAEDFRVELTGYAYRMLGSGSEAEDAVQETLLRAWKNAGRYDEERGSLRTWLYRIATNVCLDMLRSAQRRARPVDLGPAVEAGAPLGVPLPESTWVRPIADARVLPGADDPAEQAVRRESVRLAFVAALQKLSPRQRAVLILRDVLGWQAAEVAALLETSEASVTSALQRARASVRRDAPVAPLEPDAEALLDRYCRAFARYDVTAMIALLHEDATMSMPPFTWWLRGREAIADVLRHGGGSCEGARMVPVAMNGTTAFWQERPTGPGGAFEPFALMLFETAGGLITEIVTFLDERPMSSGPLPRIPSENEEDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2T3</accession>
    <name>U5W2T3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362667.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20115"/>
    <dbReference type="EnsemblBacteria" id="AGZ42295">
        <property type="protein sequence ID" value="AGZ42295"/>
        <property type="gene ID" value="AFR_20115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4080"/>
    <dbReference type="eggNOG" id="COG0638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431887_0_0_11"/>
    <dbReference type="OrthoDB" id="1200496at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="178" mass="18755" checksum="95CD795F5623BA0E" modified="2014-01-22" version="1">MTAIVGLAQSGSVYIGGDSAGVSGMSLTVRADTKVFRKDRYLFGFTTSFRMGQLIRYSLALPKPTGGLDAFMATTFIDAVRECLKAGGWAAKENDREEGGTFLVGVKGRLYAVYDDYQVAKAADGFAAVGCGDQIALGALYATAGTALTPRRRVKTALAAAERFSAGVRGPFVCLKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3V4</accession>
    <name>U5W3V4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase_M6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21950"/>
    <dbReference type="EnsemblBacteria" id="AGZ42660">
        <property type="protein sequence ID" value="AGZ42660"/>
        <property type="gene ID" value="AFR_21950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4450"/>
    <dbReference type="eggNOG" id="COG4412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_395692_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008757">
        <property type="entry name" value="Peptidase_M6-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05547">
        <property type="entry name" value="Peptidase_M6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665516" description="Peptidase_M6 domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M6" evidence="2">
        <location>
            <begin position="487"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="696" mass="75549" checksum="7F227F124374BFF5" modified="2014-01-22" version="1">MARLRLAAVALLVLPLLPLTTAAEAAPATARPPADDTRELTRERLDVSKLGPTGQAFERRARIAAAPTPPVGTVRQWLGLDDVDGGLYRKDYTLRGVGEHTEVWVAKDIAFPAGDCRAADSTEITDQQVKDLIKEFDTKIWPRETAAFSTPQERDGTDPRLTGADYTGDGAKTVTLVDNVRDDNYYEFPQAPTYIAGFFSAQINELVDRNVMTIDAYDWKHRSGANPPDQPTDDLCTSRPARPRMYEGTFAHEWQHLLQSYTDPGEDVWVNEGLSDYAQSLVGYVDGNATVHHAGNDTHLACFQGFGVVKTPYNTNPRDCGGAQNSLNVWDEGAPSEVLADYGNTYQFMLYLRDRFGAAALSRLHRDGTHQGLAGVAAALGDVQLYGVLHDFQTMTLVDKLIGDTPRGTMIGVPHARVTTPSVRSTVNLANPASYDYPGAAPNGADYVRLRDAKNKYLKGAELKSVTFTGATTLPPQTLQWKVQDGALFSGNTKDLDVAAVTEVTVPAADPVLRLRTKYATEEGYDYGYVTVSMDGGRTYTAIAGDSTVAAPLGQAINGDSAGFQPRTYDLSAYAGKKVLLGFRYVTDSAIDEGGWWIDDIKVGGAVVSDGATLDGFRSPTQIVPLRVHAWNVRLVGLDEKGRRARQVPVEQFAALKGYDKVVAVVAYDEPTGQIEQYAPYTLTVNGVVQPGGRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Z8</accession>
    <name>U5W3Z8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_22175</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363076.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22175"/>
    <dbReference type="EnsemblBacteria" id="AGZ42705">
        <property type="protein sequence ID" value="AGZ42705"/>
        <property type="gene ID" value="AFR_22175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4495"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="143"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="23135" checksum="22C35B3F247E05DE" modified="2014-01-22" version="1">MIRVFLLDDHEVVRRGLAGLLEAAGDIEVVGESGSAQEAARRIPALRPDVAVLDARLPDGNGIDVCRDVRAVDSTIKGLILTSYEDDEALFAAIMAGASGYVLKQIRGTDLVDAVRRVAGGQSLLDPAVTQRVLERIRHGVEQPRELASLTDQERRILEHVAEGLTNREIAGKMFLAEKTVKNYVSSLLAKLGLERRTQAAVLATKLLGEHPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W446</accession>
    <name>U5W446_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560325.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28635"/>
    <dbReference type="EnsemblBacteria" id="AGZ43988">
        <property type="protein sequence ID" value="AGZ43988"/>
        <property type="gene ID" value="AFR_28635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5806"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_0_1_11"/>
    <dbReference type="OrthoDB" id="1064289at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="10"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="324" mass="35428" checksum="183E943D9D6F23F9" modified="2014-01-22" version="1">MSVDVLPRLSVVVPVFNEEDVLPLFAERMRPVLDALDVSYEVLAVDDGSRDATPAVLEQMRRAWPQLRVVRLRRNAGHQNALTAGLHRARGRYVVSIDVDLQDPPEVIADMLILAEAGQLDVVHGVRTDRSTDSPFKRWTAGLYYRLIRRVVGTAVPHNAGDFRLLSRTAVDALTELPEQLPVYRLLVPWLGLPSGEVEYAREKRAAGRSKYPLAKMIRLALDSITGFSAAPLRVATWLGSAGVVLCFVLGAAVLAAYLWGTVVPGWTSMFVALVFLGALQLLCLGLLGEYVARIFTSSQGRPAYVVTFDSAVTGTRRPAGVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Q6</accession>
    <name>U5W4Q6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_29190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29190"/>
    <dbReference type="EnsemblBacteria" id="AGZ44099">
        <property type="protein sequence ID" value="AGZ44099"/>
        <property type="gene ID" value="AFR_29190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5914"/>
    <dbReference type="eggNOG" id="COG3507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016508_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041542">
        <property type="entry name" value="GH43_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17851">
        <property type="entry name" value="GH43_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14200">
        <property type="entry name" value="RicinB_lectin_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665536" evidence="4">
        <location>
            <begin position="44"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="5">
        <location>
            <begin position="49"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="689" mass="74870" checksum="8386120C2C705359" modified="2014-01-22" version="1">MERTMRTNGEAPRRRRLLQAVLTVMTVLAGGFGVAVTATEAGAATIDTGASYVLVNRNSGKALDVYNLATTDGARITQWSRNDGAQQQWQFVDSGGGFYRLRSKLSGKVLDVANRSTADGAAVVQWADGNGTNQQFSVQDIDGYIQLINRNSGKALEVQGASTADGGNIVQYADWNGTNQQWQLVPVGGTPTGGTFTNPVVWQDFADGDIIRVGDVYYYSASTMHYSPGAPVLRSYDLVNWEYAGHSVPRLDFDSNAYDLNGGRAYVKGIWASAFNYRPSNSTYYWLGCTEFNRTYVYTSANAGSGWGKKARINKCYYDAGLMFDNDTPYVAYGNGTISVAQLSSDLTSEVRSQTVYQTPSSIGTLEGARMYKRGNYYYIWLTRPANGQYVLRSTSPWGPYEQKQVLLNLPGPISGGGVPHQGGLVQTQNGEWWYMAFTDAYPGGRMPTLAPITWSADGWPVLQTVNGRWGASYPRPNLPWHPVTSMTGTDTFTGTTLGPRWEWNHNPDNSKWSAGSGLRLSTATVTNDLYAARNTLTHRIQGPSSTATIELDYSPMANGDRTGLAMLRDSSAWIGVKRDNGATRVTMVNGLTMDGSWNTTGTGTEVAGAAVSGGRIWLRASADIRPGSGRQATFSYSTDGVTFTALGPAFTLNNAWQFFMGYRFGLFNYATQALGGAVTVRSFALTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4S5</accession>
    <name>U5W4S5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DJ-1/PfpI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29290"/>
    <dbReference type="EnsemblBacteria" id="AGZ44119">
        <property type="protein sequence ID" value="AGZ44119"/>
        <property type="gene ID" value="AFR_29290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5935"/>
    <dbReference type="eggNOG" id="COG0693">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070319_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="1">
        <location>
            <begin position="22"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="24378" checksum="F139987E3FFDCE5F" modified="2014-01-22" version="1">MTGARYWTLKNGHRHPTGYWAEEFVSPYRAFTEAGHHITVATPGGVVPAVDTMSLRASMAGSEETSLELEAVIEAAEELRRPAVLRDVRLDDYDAVFYPGGHGPMEDLAFDLDSGMVLRKALASGKPLGIVCHAPAGLLATRDEHGHTPFADYRVTGFTNDEEAAVGFADKAEWLMEDRVRDLPTQYSRGPAWEVYTVVDRNLYTGQNPASSGPVAVEMLKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W557</accession>
    <name>U5W557_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560484.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29430"/>
    <dbReference type="EnsemblBacteria" id="AGZ44147">
        <property type="protein sequence ID" value="AGZ44147"/>
        <property type="gene ID" value="AFR_29430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29430"/>
    <dbReference type="eggNOG" id="COG3878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056726_2_0_11"/>
    <dbReference type="OrthoDB" id="1878535at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015315">
        <property type="entry name" value="DUF1963"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035948">
        <property type="entry name" value="YwqG-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09234">
        <property type="entry name" value="DUF1963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103032">
        <property type="entry name" value="SSF103032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="31399" checksum="593B119D07D9FFF1" modified="2014-01-22" version="1">MTLEEYKTKIRTIARSLEISEPITEAVIDLIRPHIELFRHEGGTAGQFGGNPALPDDVPWPVDGEFPGSPEEPVPFAASINCSQLPLDILDIDLPRDGQLLFFSTLDFDDQLGSIVHVPAHATARERHAPEDTYPAPMHSSTLGARVGWSYPYEGPILEDLYRQSSEDHPYHHEDFQRLIKEGPMPDECLIQIGGYFEAPQGHPLWMVEGAPEDWVVLAKITVRDGFRSNTEEEASEYWRNDDADPDGNECCFFYTIRTTDLAGRHFDRAATFLQEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W584</accession>
    <name>U5W584_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PLD phosphodiesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560509.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29555"/>
    <dbReference type="EnsemblBacteria" id="AGZ44172">
        <property type="protein sequence ID" value="AGZ44172"/>
        <property type="gene ID" value="AFR_29555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29555"/>
    <dbReference type="eggNOG" id="COG1502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_531899_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="1">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="515" mass="57094" checksum="08AB4D81E5949529" modified="2014-01-22" version="1">MNELLAGSPGQVIDLGRRTVDGAFFLRRQDGPPIRSPFIPRDTGSQDGVRHCLTYEGGGSNIREELVRLIDSATRRVFVATLFLGDEQVRQALERAADRLQGGVYVVSALDDRGLNKAINDASDTLDVDTQIEFRNFTRLTTSGIYVRGYPGLHAKFAVVDDARALVSSANLVTRSFTCVGENGVVLEGVDEVAVLAWAFRRLWRQSPWDMPPAPSYHSVSDRSPADDALNGVPETSRLLWTWQEQHRILDDIRRTIDQAEHELILGTFSIGNMTFDMPKAPARPEILYDPVCRAIERGVRVRMLLRGRNNAESSRAEAAAFHAAGVEVYADTLTHAKGCLADRRIGSLFSANFMTTMGLTGGMELGMRLDGTGALGEAWRYFEHYMAETDLELAVNPPAGVTADRLLADSLRRWPLPGHGFVSCNDQTWAQLATHTGPALWEQDGDHHLVFSGARCYRLIGEGARWRMEPANRPAGSRNIRQLIEGWISPRRGNRSTDTNRRGIFAPVLRRASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5P3</accession>
    <name>U5W5P3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43310">
        <property type="protein sequence ID" value="AGZ43310"/>
        <property type="gene ID" value="AFR_25220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5111"/>
    <dbReference type="eggNOG" id="ENOG5031PUV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2467600_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="9219" checksum="3ED64EE613BBAFA7" modified="2014-01-22" version="1">MTVGDVVTVPERYGLGPIEVTAIASAEVGLTASLTGSGYSMSGCSGGGGVFAPGGGGVEMSCRVGVVATINDAMTLEVVEIDDRAAVLRIAAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W656</accession>
    <name>U5W656_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmB</name>
        <name evidence="2" type="ORF">AFR_31695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31695"/>
    <dbReference type="EnsemblBacteria" id="AGZ44599">
        <property type="protein sequence ID" value="AGZ44599"/>
        <property type="gene ID" value="AFR_31695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6415"/>
    <dbReference type="eggNOG" id="COG0220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050910_0_1_11"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01057">
        <property type="entry name" value="tRNA_methyltr_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="PTHR23417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00091">
        <property type="entry name" value="TIGR00091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="184"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="207" mass="22832" checksum="C4975078499843D8" modified="2014-01-22" version="1">MGSRHAEAMTHVWPAYGLTVLDGDRTPLDLETLFGREAPRVLEIGFGMGDATAEMAAADPGRDYLAVEVHTPGIGNLLALVGERGLTNVRVAHGDAMELVHRIPESSLDAVHVFFPDPWPKARHHKRRLIQPANVALLRDRLRAGGLLHCATDWAHYAESMAETLEADPGLVAAPDDEARRPETKFERRGVASGREIFDLMFRRGYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W701</accession>
    <name>U5W701_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33305"/>
    <dbReference type="EnsemblBacteria" id="AGZ44919">
        <property type="protein sequence ID" value="AGZ44919"/>
        <property type="gene ID" value="AFR_33305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6740"/>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_485425_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031768">
        <property type="entry name" value="CBM60_xylan-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16841">
        <property type="entry name" value="CBM60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666151" description="CBM60 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="CBM60" evidence="2">
        <location>
            <begin position="74"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="561" mass="58445" checksum="CA769E8668EE270B" modified="2014-01-22" version="1">MVSTVLATAGAVFLVPPTAAFADARGEAETGRITPAANGAFPADVTAGGGHAAEIWDRASLEVPVTTTAPATAVRVRVRQELCGAEPPWIRVHVDGVFVQTATVEPTNWTTIPFRGTWAAGPHTVRIDFTNPYYNAATCGARELFVDHVSFASAVPGRTYYVDAADGDDTNAGTSADAAWQTIGRVNDATLAPGDSVLFKSGQTFTGETLTAGQPGNTTARVTYGAYGTDTDPIFDGEGQRYPVVVNAPFVTVQDIQVRNAGDEYKIGMRVFGADALVQRITASGNAIGVQAEDGADRLHLTASRLVDNKTVINPDGLGKAQGSTDDYGASGVVVLKAHGVEIDENTMSGNIGPSADFDEDGSAVEIYGAVGTVVHHNRSIDNQTFSEIGHAETRNTTFHNNLIAANAGIDEAIGFNAQGNGKFGPVLNTRMFNNTVVLLGADSKGVVVGTGATAFLHNNIVQADYAGWSGDQKIDEGHNVYRPGAWGNDIWSDANTGQGIAPTSVTADPLFVGSGDYHLRAGSPAKDRGVSAYGSTWDLDHVARTAGTGTDAGAYELPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W721</accession>
    <name>U5W721_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Activator of Hsp90 ATPase 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33405"/>
    <dbReference type="EnsemblBacteria" id="AGZ44939">
        <property type="protein sequence ID" value="AGZ44939"/>
        <property type="gene ID" value="AFR_33405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6761"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_6_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="13147" checksum="93D3CA3E57ED2B3F" modified="2014-01-22" version="1">MLFDCMTQPEHLTHFWGPDGTTTPLENIVVDLRPGGVFETTMVNDADGSAYTMRAVYEVVHRPERLVWLDRGIRTTVTFVDLRDGRTEVVTHQTEAPQASAGFLSSLDRYAGYVAGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7S0</accession>
    <name>U5W7S0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative PfkB-family carbohydrate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_34985</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561589.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34985"/>
    <dbReference type="EnsemblBacteria" id="AGZ45253">
        <property type="protein sequence ID" value="AGZ45253"/>
        <property type="gene ID" value="AFR_34985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7079"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_2_2_11"/>
    <dbReference type="OrthoDB" id="1030724at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="5">
        <location>
            <begin position="8"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="30574" checksum="AE7C3DD984ACD91D" modified="2014-01-22" version="1">MKPPVATVVGQLARDLVLSVGAVPDTGAAADATDRREQLGGKGANQAVGLAQLGVVPRLVAVAGEDIIGDVLLDQARRDGIDVTAVVRRPGALTGLIVELLDSDGQWRYVQHLSEEVLLTVRDVERARDVISGADAVLVQLQQPAAAALAAARIGHDAGRLVVLDGVPEEHRDELLAAADVVRADDQEARLLLDGPVTPAAARRLLDDGPRLLALGVEDGNLFVWKDGHVRLPLTGGKPVDTTGGGDAFTAALTASLLRGEDYSLAARWAVAASGATVTHVSGRPSLAGLQVSADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7S8</accession>
    <name>U5W7S8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35035"/>
    <dbReference type="EnsemblBacteria" id="AGZ45263">
        <property type="protein sequence ID" value="AGZ45263"/>
        <property type="gene ID" value="AFR_35035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7089"/>
    <dbReference type="eggNOG" id="COG1718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075702_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="254" mass="27826" checksum="EC1C061A64970EEA" modified="2014-01-22" version="1">MIGVLPEVTAIRYVTPLREGGSLPGIVEADNLGTYVVKFRGAGQGPKALVAEVISGELARRLDLPVPELAVTHLDPIVARAEPDEEVQELIKASAGANLGMDYLPGALGYDPVAHPVEARLASRTLAFDAFVENVDRSWRNPNLLVWHGELWLIDHGATLYFHHNWPRAEKVVHRAYKWDDHVLRPYATELATAGPELAAQLTPQVLEEVIGLVPDEWLEEFDFATADEARAAYLGHLRERAADPSAWLPGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Z8</accession>
    <name>U5W7Z8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561469.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34370"/>
    <dbReference type="EnsemblBacteria" id="AGZ45132">
        <property type="protein sequence ID" value="AGZ45132"/>
        <property type="gene ID" value="AFR_34370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6954"/>
    <dbReference type="eggNOG" id="COG4319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128775_0_0_11"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024507">
        <property type="entry name" value="DUF3225"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11533">
        <property type="entry name" value="DUF3225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="123" mass="13451" checksum="7FE2E339413D22C6" modified="2014-01-22" version="1">MQINRPDIVDEVAAAFADYEDALVANDVDRIIAFFTDDAVRFGVADQQVGLAEQTAWRKAQPPLPPGRRLKDTIITTYGADVAVVTTLFGYPGSDTLGRQSQSWLRLPSGWRIITAHVSHPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W851</accession>
    <name>U5W851_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Inositol-phosphate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561524.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34655"/>
    <dbReference type="EnsemblBacteria" id="AGZ45187">
        <property type="protein sequence ID" value="AGZ45187"/>
        <property type="gene ID" value="AFR_34655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7011"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_0_0_11"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28002" checksum="DA05EFA0F1010E9A" modified="2014-01-22" version="1">MLITDSELAVGAAAAGADVVRSMFGESLRRFGKDGDDFATEADLEAERVIIETLRAARPGDAVVGEESGRSGGSTGRTWLVDPLCGTLNYAVRNMLVAVNVALRDGGDVAVAASADPFANEIFWTDGDGAYVRTGSGDERLEPSAASRLVDFNLDPPFPNAPGFRAVDLLGDPRFVERFRPRVVSTTLAVAWVAAGRRAAYVTDGHLRDSVHFAAGIALCRAAGCVVTGIHGQPLHTGAGGLLVAADEETHAELLAINSVEGRRTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8D6</accession>
    <name>U5W8D6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="9">CTP synthetase</shortName>
            <shortName evidence="9">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">pyrG</name>
        <name evidence="12" type="ORF">AFR_29895</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560577.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29895"/>
    <dbReference type="EnsemblBacteria" id="AGZ44240">
        <property type="protein sequence ID" value="AGZ44240"/>
        <property type="gene ID" value="AFR_29895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6055"/>
    <dbReference type="eggNOG" id="COG0504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_11"/>
    <dbReference type="OrthoDB" id="783657at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP_synth_N" evidence="11">
        <location>
            <begin position="9"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="GATase" evidence="10">
        <location>
            <begin position="312"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="9">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-glutamine binding" evidence="9">
        <location>
            <begin position="393"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="9">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="524"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="20"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="158"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="198"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ44240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="572" mass="62301" checksum="022090ABA6BD6628" modified="2014-01-22" version="1">MAATARETRHIFVTGGVASSLGKGLTASSLGNLLTARGLRVVMQKLDPYLNVDPGTMNPFQHGEVFVTEDGAETDLDVGHYERFLDRALAGKANVTTGQIYSSVIAKERRGEYLGDTVQVIPHITNEIKERIFAMADPDEYGRTPDVVITEVGGTVGDMESLPFLEAIRQVRHEIGRDHVFYLHVSLVPYLAPSGELKTKPTQHSVATLRNIGIQPDALVCRSDREIPEKLKHKLSLYCDVDREAVVACPDAPSIYDIPKVLHREGLDAYVVRRLGLSFRDVDWSSWDDLLERVHHPRRTITVALVGKYVDLPDAYLSVSEAIRAAGFANHARVQLRWVPSDDCDTPAGAAAALKGVDGIVIPGGFGIRGIEGKINTSRYARENGIPILGLCLGLQCMTIDAARNMAGLEGANSLEFDEKAPHPVISTMADQQDIVAGKGDLGGTMRLGAYPAKLVEGSVVAEAYESTEISERHRHRYEVNNAYRDRLAKAGLTFSGTSPDGRLVEFIELDRDKHPFFVATQAHPELKSRPTRAHPLFAAFVKAAVEYAAADELPVEVAEGVKAEKAAVAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8K2</accession>
    <name>U5W8K2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_29975</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29975"/>
    <dbReference type="EnsemblBacteria" id="AGZ44256">
        <property type="protein sequence ID" value="AGZ44256"/>
        <property type="gene ID" value="AFR_29975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6071"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="3">
        <location>
            <begin position="168"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="427" mass="43952" checksum="6958EB5497B62A11" modified="2014-01-22" version="1">MRGARSYLLDGLLATGLLAIGLVGTAPAGRNQDITSISAWAYALVITAALAMALRRLAPTTALTVCAAAVSVYLVGGYPFGPILLSLAVAVYTVGAHRPFRQAAIAASAVYAAVAIGGLTGGGLGLDAVIPQLAWIAGPLAVGVTVRSGREQQLQSRRDEARRQADAERLRVAQEVHDVVGHGLAAITMQAEIALHLLAKQSSQAPLAPQPSQPPLPPQPSQPSQPSQPSQPSQPSQPSQPSQPSQPSQPSQATQPTQPSQPSPVEAALAAISRTSRESLDELRATLGAVRRGDDFDDRSPVAGLARLPALVDRTRAVGVPVKVDVIGPLANLPVAIDLAAYRIVQESLTNVLRHAGPASASVSITRTDDHVTVSVNDTGRDAYPTVTPGHGLSGMQERVSALGGSLSAGPTPEGGFTVTAELPVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8L6</accession>
    <name>U5W8L6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MDMPI_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35480"/>
    <dbReference type="EnsemblBacteria" id="AGZ45352">
        <property type="protein sequence ID" value="AGZ45352"/>
        <property type="gene ID" value="AFR_35480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7180"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051661_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017517">
        <property type="entry name" value="CHP03083_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017520">
        <property type="entry name" value="CHP03086"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024344">
        <property type="entry name" value="MDMPI_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11716">
        <property type="entry name" value="MDMPI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03083">
        <property type="entry name" value="TIGR03083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03086">
        <property type="entry name" value="TIGR03086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MDMPI_N" evidence="1">
        <location>
            <begin position="16"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="184" mass="19756" checksum="16CCD4301E1111C9" modified="2014-01-22" version="1">MQEHALAALAAPPTLAVVRAIEPGQLGNPTPCTEFDVRALVEHMLHWGPALARASRHSDGEAPADGDLLERLERQIGQLVEAWSVPAAWEGTTSMGGPQELPAGMIGAMAVGEIVVHGWDLARATGQEISWDPGLQDFLYDEVAKTAELGREMQAYGREVPVPPTAPTLDRMLGLTGRDPAWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Y9</accession>
    <name>U5W8Y9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">DNA ligase</fullName>
            <ecNumber evidence="10 11">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ligA</name>
        <name evidence="14" type="ORF">AFR_36995</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="10 11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561989.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36995"/>
    <dbReference type="EnsemblBacteria" id="AGZ45653">
        <property type="protein sequence ID" value="AGZ45653"/>
        <property type="gene ID" value="AFR_36995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7484"/>
    <dbReference type="eggNOG" id="COG0272">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007764_2_1_11"/>
    <dbReference type="OrthoDB" id="241401at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00114">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018239">
        <property type="entry name" value="DNA_ligase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033136">
        <property type="entry name" value="DNA_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNAligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="DNA_ligase_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="SSF52113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01055">
        <property type="entry name" value="DNA_LIGASE_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01056">
        <property type="entry name" value="DNA_LIGASE_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 10 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10 11" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="BRCT" evidence="13">
        <location>
            <begin position="633"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="721"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="10">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="116"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ45653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="744" mass="80915" checksum="264CEF7C1AD6F45C" modified="2014-01-22" version="1">MPENQSVETGSAAAAEQLAEGRPTAEQVAAAGAEPTSEASARHAELSDELRGHQYRYYVLDSPTISDAEFDKLLRELEALEERFPALRTPDSPTQNVGGTFSTLFTPVEHAERMMSLDNVFDEDELAAWAERTERDAGAPVRFICELKVDGLAINLTYEKGRLVRGATRGDGRTGEDVTSNVRTIREIPERLADDNPPELLEVRGEIYFPASAFADLNASLVEQGKAPFANPRNAAAGSLRQKDPRITASRGLRMVVHGIGARKGFRPDSQSHAYEALKSWGLPTSERWRLVDGMDEVRDYIAYYGKNRHSVEHEIDGVVVKVDSVAIQGRLGSTSRAPRWAIAFKYPPEEVTTKLLDIAVNVGRTGRVTPYAVLEPVLVAGSTVAQATLHNAREVERKGVWIGDTVVLRKAGDVIPEVLGPVMDLRPDDAHPFVMPTVCPECSTPLAPAKESDIDIRCPNTQHCPGQLRERLFYLASRDALDIEVLGYKAARALYESGILTDESDLFSLTADDLLRSPFFVNMDGTLGSNAVKLLENLEEVKQRPLWRVLVALSIRHVGPKASRALALEFGSMEKIEAAVEDGPIEDLSAVDGVGPTIAESLREWFSVDWHREIVRKWREAGVKMADEHSESGPRPLAGLTLVVTGTLSTHTRDEASEAIMAGGGKVSGSVSKKTGFVVVGDNPGSKYDKALSLRVPVLDEAGFAVLLADGPEAARAVAEISEEPVKEPKKKAAAKPSNEEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9A1</accession>
    <name>U5W9A1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP synthase epsilon chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP synthase F1 sector epsilon subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">F-ATPase epsilon subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">atpC</name>
        <name evidence="10" type="ORF">AFR_37325</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562054.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37325"/>
    <dbReference type="EnsemblBacteria" id="AGZ45719">
        <property type="protein sequence ID" value="AGZ45719"/>
        <property type="gene ID" value="AFR_37325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7551"/>
    <dbReference type="eggNOG" id="COG0355">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084338_4_0_11"/>
    <dbReference type="OrthoDB" id="1696893at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="PTHR13822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="SSF51344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01216">
        <property type="entry name" value="ATP_synt_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7 8" id="KW-0139">CF(1)</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 7 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_DE_N" evidence="9">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="93" mass="9650" checksum="11EDA2AC0AE38F56" modified="2014-01-22" version="1">MANQLHVQVVAVEEKVWSGDAEMLVARTTEGELGVLPGHSPLLGLLKEPSQVRVKLAGGEQLTYDVTGGFLSVDANGVTVLAESATAATPDAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9E1</accession>
    <name>U5W9E1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562099.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45764">
        <property type="protein sequence ID" value="AGZ45764"/>
        <property type="gene ID" value="AFR_37550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7595"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_0_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="29"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="28216" checksum="551B13E22985C2D8" modified="2014-01-22" version="1">MAGLLGDFLKARRALLLPGDVGLPGYGRRRVAGLRRDELAGLAGVSAHYLMRLEQGRDRHPSPQVLDALARALRLDADTTAHLHALAQPPAFPPPRPLDPAVQRLLDDWAGTPAYVRGRHLDVLAGNKLAAALSPMYAAGRNLARDIFLEPEARKLFADWEVIAEQTVAALRAGADPRDPALAALVAELDRDPDFRRWWARHDVRPARDETKRFHHPVVGPLTLRRQALTVAGALDQVIIAYQAEPGSDSAKLLSRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9F7</accession>
    <name>U5W9F7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37650"/>
    <dbReference type="EnsemblBacteria" id="AGZ45784">
        <property type="protein sequence ID" value="AGZ45784"/>
        <property type="gene ID" value="AFR_37650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7615"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048587_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="51"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="300" mass="31832" checksum="E63855DC7C022F20" modified="2014-01-22" version="1">MDTIRTEEVTFASDGLELAGTLRVPDAADGSAVVLTGPFTGVQEQVTGLYADRLARQGLITLAFDHRGFGRSAGRRGHEDTQGKLADLRSAVGLLQERPEVAADRIGVVGICLGGGYAVSAAATDPRLRAVAGIAGAYNSPAWFAADPSGYRAALRGFLDSYDDFLPAVAPDHGEAAMGGDEPWDYYGTSRSAAKSWENRVTRGSLHSLMTFDALGRAALLAETPLLIVHGRQDDYCSPELAARMHAETPGDKEIVWLDAQKHIDLYDAEPYVTQAVEATTVFLRRTLRAGAATAVPAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9J6</accession>
    <name>U5W9J6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Silent information regulator protein Sir2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_31925</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560978.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31925"/>
    <dbReference type="EnsemblBacteria" id="AGZ44645">
        <property type="protein sequence ID" value="AGZ44645"/>
        <property type="gene ID" value="AFR_31925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6462"/>
    <dbReference type="eggNOG" id="COG0846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_3_2_11"/>
    <dbReference type="OrthoDB" id="1264438at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="29881" checksum="9412D2F8B5270252" modified="2014-01-22" version="1">MTQIEQLVEWTGDGGVVVLSGAGLSTESGIPDYRGPSGAARRSATPMTYQAFTRDPVARRRYWARSHLGWRTIGDARPNDGHRAVARLQELARVDGIITQNVDGLHQAGGAHGVVELHGNLSRITCLDCGELTAREEHSERLDAANAGFAAAVTAVNPDGDVDIDDAELDGFTVVDCLACGGMLKPDVVYFGETVPPARVSRSFELVAGARTLLVLGSSLTVMSGRRFVLRAAKDGIRVAIVNQGVTRGEPYAGLIVDAALGEVLPELVRRIEGAGRFAPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9L5</accession>
    <name>U5W9L5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="3">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_32025</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560998.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32025"/>
    <dbReference type="EnsemblBacteria" id="AGZ44665">
        <property type="protein sequence ID" value="AGZ44665"/>
        <property type="gene ID" value="AFR_32025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6483"/>
    <dbReference type="eggNOG" id="COG0188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002977_6_1_11"/>
    <dbReference type="OrthoDB" id="217468at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034335">
        <property type="term" value="F:DNA negative supercoiling activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="8">
        <location>
            <begin position="26"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="796"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="840" mass="90491" checksum="D98557BC72BE20D0" modified="2014-01-22" version="1">MARRKNEQSRVDLSAFDQAGARIIDNPLTTEVEDSYLEYAYSVIHSRALPDARDGLKPVHRRILWSMQEQGHRPDRAYVKSARVVGDVMGKYHPHGDLAIYDAMVRLAQDFSLNAPLIDGHGNFGSPDDGPAASRYTEARMSREAMLLVGELGEGTVDFKPNYDGSELEPRVLPAAFPNLLVNGTSGIAVGMATNMIPHNLGEVVAAARHLITTPEADLDALMRFVPGPDLPTGGQLLGLDEVRRAYETGRGVVRMRARAQTGPLEGGRGRQAITVVELPYGVGTEKIIEAITDEVKGKLITSGPRKGQRQPARLQGIADVKDLTDREHGIRLVVECKVGVNPQALLADLYRLTPLESSFGINNLVLVDGQPQTLGLKALLEVFLAHRYEVVTRRTTHRRTKRQDRLHLVDGLLIALIDIDRVVAIIRGSDDAAAAKQGLMTEFGLTDIQATYILDTPLRRLTRFDRIELETEQERLRTEIAELSRILDNNDVLKQVVSDELAEVASEFGGPRRTTLIDGDLKEVLAASVPAGPLEVADDPCQVILSATGLIARTAAESEEATEGRRRSGRVKHDAVRAVVHSTARGRVLLVTSTGRAFKVDVLPLPVLPEQSGTVSVRGGMSASELIPLEPGEKVVGLAPLTAEGSPGLALGTRQGVVKVCAPEWPVRSDEFEVITLKDGDEVLGATWLTDGSESVAFVSSDASMLRFPAKLVRPQGLKGGGMAGMNLSSGADVVSFNVVNTTDAQHGEPMVVTSTGTQVKVTPFSTYPAKGRATGGVRVQRFLKGETRLTVSWVGPRPVGASSTGDPVELPATDPRRDGSGEAVLMGPQIIGHLIERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9M3</accession>
    <name>U5W9M3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562243.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38270"/>
    <dbReference type="EnsemblBacteria" id="AGZ45908">
        <property type="protein sequence ID" value="AGZ45908"/>
        <property type="gene ID" value="AFR_38270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7742"/>
    <dbReference type="eggNOG" id="COG1652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1105290_0_0_11"/>
    <dbReference type="OrthoDB" id="229486at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666446" evidence="2">
        <location>
            <begin position="27"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="26681" checksum="7470257B2D816A1E" modified="2014-01-22" version="1">MFRRFVTAVLLACLAALAAPASAASAAPSAAPVKFYVVRDSFGGQPEFLFEIAQRFLGNGDRNPEIFQLNKGRLQPDGLRLTRPEAILPGWILQLPPDAKGEGVQTGALPTVAPAPSASPALRLAPAPEEGRDWWKWGILAAGAVLLLASAAATWWLWRRGDLRKPPPARTPAQEWAAGDTDTDRYYYLEDSAPPVAAPDRRPVLLLAAVAVVAVATLVAGVVFVLTSEEPSRPAAALQLGRVVGDSSLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9X0</accession>
    <name>U5W9X0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562152.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37815"/>
    <dbReference type="EnsemblBacteria" id="AGZ45817">
        <property type="protein sequence ID" value="AGZ45817"/>
        <property type="gene ID" value="AFR_37815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7647"/>
    <dbReference type="eggNOG" id="COG0586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_1_1_11"/>
    <dbReference type="OrthoDB" id="1928533at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="221" mass="23967" checksum="A2B023254751C70B" modified="2014-01-22" version="1">MSVPQYLAAETEPSQLGGLTGWVASVIESLGEVGVGLLVALENLIPPIPSEIVLSMAGYLAGSGKVNLVLVAIAATAGSVLGALALYWLGYALGEERLRRWLDRIPLVDADDLSTADRWFEKHEKGAVFFGRMAPVVRSLVSIPAGANHMQLGMFTLLTTLGSGIWNCLFIGGGYALGSRWQQLEKYSSYFDYAIMAFFVIAIGSWVVKKVRKRRRATADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA10</accession>
    <name>U5WA10_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative CorA-like magnesium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_32515</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561100.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32515"/>
    <dbReference type="EnsemblBacteria" id="AGZ44761">
        <property type="protein sequence ID" value="AGZ44761"/>
        <property type="gene ID" value="AFR_32515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6580"/>
    <dbReference type="eggNOG" id="COG0598">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007127_0_2_11"/>
    <dbReference type="OrthoDB" id="822653at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="SSF143865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="SSF144083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="324" mass="36129" checksum="3DD7390E26268A64" modified="2014-01-22" version="1">MTRLYEGGAVVAEGFGAHEISDQLAEHPKAVLWLDLFDPEESDLRSVAAEFNLHPLAVEDAVHDHQRPKLDRYPGHLFMNVYSVHVQTEGDVPVLHKGEISAFITERALITVRKSEVDLTRLIRRWDADAALSATGGVGFLVYGLLDVVVDGQFAAARELDVAMDKTEDTILEEGGAPRSVRMYGFALRKTIAALRRPVAPMADLIADVMRADTRLVDENLEPYYRDVDDHARRATETIDSARERINGLLEADLNEQSNALNDVTRKLAAWAAIIAVPTALTGYFGQNVPYWGYEKFWGFLLSTGLIVFSASGLYLYLKRRGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA52</accession>
    <name>U5WA52_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562358.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38850"/>
    <dbReference type="EnsemblBacteria" id="AGZ46024">
        <property type="protein sequence ID" value="AGZ46024"/>
        <property type="gene ID" value="AFR_38850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7861"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069431_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="26871" checksum="FC53E9207C6FBB64" modified="2014-01-22" version="1">MEIVTSGDRPDLHEKAAAAFKERWPEFIFHDATSKKYMSRVKQHFGGFDITVLDNGAVAAGGWGVPIPWSGDADDLPSGYDEALVRAVQAREAGIPATTLSFMAAAVASAYDKRGLAKTVLQELTRRAHEAGLVHVIAPLRPTWKHRYPTVSMAEYAAWARADGLSIDPWIRTHQRMGARVLGPAPRSMVIEGTVAEWEDWTGMVFPATHDYVVPDALNLVRIDVDQDRGVYVEQNLWVQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAK0</accession>
    <name>U5WAK0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562483.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39475"/>
    <dbReference type="EnsemblBacteria" id="AGZ46149">
        <property type="protein sequence ID" value="AGZ46149"/>
        <property type="gene ID" value="AFR_39475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7986"/>
    <dbReference type="eggNOG" id="ENOG50331SS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080168_4_1_11"/>
    <dbReference type="OrthoDB" id="1857091at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043231">
        <property type="term" value="C:intracellular membrane-bounded organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="24226" checksum="DE17F723823B4C01" modified="2014-01-22" version="1">MTSIALAEELLLLAYDDQTGKATGSRIGLDLGMSAAVLVDLALAGRIAYVNGNLVVNDPTPIGDPIADLVLAKAAADEPHTPAQWVQRLRHNLRNRVLQDLIARGVVRDVDETQMGHIHVHRYPTADPAYEAEIRKRLAEALITDAPPDERTAALATLLVATRMEPALRLPPEEAARAHDRLEQIANGAGFSGTVNIEDSIVRPSVALVVATLGRAIQAALGAPKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAL3</accession>
    <name>U5WAL3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fructose-bisphosphate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561367.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33860"/>
    <dbReference type="EnsemblBacteria" id="AGZ45030">
        <property type="protein sequence ID" value="AGZ45030"/>
        <property type="gene ID" value="AFR_33860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6854"/>
    <dbReference type="eggNOG" id="COG1830">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057069_2_1_11"/>
    <dbReference type="OrthoDB" id="1560751at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="29625" checksum="B1DA2B89771C5B05" modified="2014-01-22" version="1">MDSRTGKKIRLGRLLDPRTGRAVVVAASHGVMSGPPAGLRTRAEIETAFSKMGTADGVMVSPGMLPLLEDFYVGRGRPALVLHLDWKNHGRKIYTPGQDGRGEGVLAQLASLEDVAAAGVDAVMTYLYIGQRNSELERAEIERNARIVADAAKVGVAVIIEPRSALEAEGPEALSTEVLSLYCRISADIGADLVKVLWNGSVEAFAPVAQTCYAPILVAGGPGGEDEKDTLQLAADSIEAGAAGVMFGRRVFRAERPEAVLAGLRAVTHDGVSVDEALARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB08</accession>
    <name>U5WB08_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hag</name>
        <name evidence="6" type="ORF">AFR_40400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562666.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40400"/>
    <dbReference type="EnsemblBacteria" id="AGZ46334">
        <property type="protein sequence ID" value="AGZ46334"/>
        <property type="gene ID" value="AFR_40400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8177"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011142_2_2_11"/>
    <dbReference type="OrthoDB" id="636786at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001492">
        <property type="entry name" value="Flagellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046358">
        <property type="entry name" value="Flagellin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042187">
        <property type="entry name" value="Flagellin_C_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42792">
        <property type="entry name" value="PTHR42792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00700">
        <property type="entry name" value="Flagellin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00207">
        <property type="entry name" value="FLAGELLIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flagellin_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Flagellin_C" evidence="5">
        <location>
            <begin position="195"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="29605" checksum="E1387D8E6742E40D" modified="2014-01-22" version="1">MGLRINQNIAAQNAYRSLSTSDSQMSKSLEKLSSGFRINRAADDAAGLAISEGLRSQVGGLKVAVRNAQDGVSVVQTAEGALNEVHSILQRMRDLGVQAGNDSNNTEARANIATEVTSLTSELGRIADSTNFNGTKLLDGTAGVNGDGKMKFQVGADGDVNSQIEVDLTSANVKDIASTLGTLSFTDADAAQASINAIDTQVKAISTARASLGAYQNRFEHTINSVNVAIENLSASESRIRDTDMAQEMMSFTRSQILTQAGTSMLSQANQSQQGVLSLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBC5</accession>
    <name>U5WBC5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561616.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35120"/>
    <dbReference type="EnsemblBacteria" id="AGZ45280">
        <property type="protein sequence ID" value="AGZ45280"/>
        <property type="gene ID" value="AFR_35120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7106"/>
    <dbReference type="eggNOG" id="ENOG503469M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1618025_0_0_11"/>
    <dbReference type="OrthoDB" id="1752146at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019692">
        <property type="entry name" value="DUF2581"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10756">
        <property type="entry name" value="bPH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="16876" checksum="6F1FDCB934F50F39" modified="2014-01-22" version="1">MDAARFSPTWQQALGRGLYLGVLTAVVALLVTVVALSLTPSAPPHWAWPLLAFGPPLAGVLVGALVRPRVGTRVDARGIRTVTPFGEGVEPWSEVVDLRAERRGARTVVSVYLESGASVQLSAPYSGELFASDPQFELKMFALSHLWRSHRFGGVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBG0</accession>
    <name>U5WBG0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_40365</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40365"/>
    <dbReference type="EnsemblBacteria" id="AGZ46327">
        <property type="protein sequence ID" value="AGZ46327"/>
        <property type="gene ID" value="AFR_40365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8169"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="200"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="257"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="515" mass="53205" checksum="654371B1034983FD" modified="2014-01-22" version="1">MEMGIAKRLTLIVLVGALAIVGLTINTVSGQRSLVGQADLVRVLEAGLAALNHLDTRQSELKVDGYRAALGDDVTTDVADDVVSATEAADAVEAVGLPADLAAEFATIRPNVDSFGTFMADFVKQAGAGGDAAEANRDQIAQRNNAVDDQLGALNEKVAAAAEQERADMNSTINRISLITYLFSGIGLLLLIGLSVPLARSILRPVRRLGEVIDALAKGDLTQRSGITSRDEVGRMAVGLDSALSSIHRSLSTIGQNAETLASSATELSAVAGQIAESAHDTDAQTSSASNEAEEISRNVQTVAAGSEEMGLSIREISRNTSEAAQIAAVAVTEAAQATETIRQLGESSAEIGNVIKLITSIAEQTNLLALNATIEAARAGDAGKGFAVVASEVKDLAQETARATEDIGSRVTAIQQDTSGAVAVISRISEVIARINDFQTTIASAVEEQTATTGEMSRSISEVASGSSRIAANIADVSTASGSAVHGVTQTRQASEEVARTAEELRALVGAFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBU3</accession>
    <name>U5WBU3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative subtilase-family protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_41065</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41065"/>
    <dbReference type="MEROPS" id="S08.026"/>
    <dbReference type="EnsemblBacteria" id="AGZ46467">
        <property type="protein sequence ID" value="AGZ46467"/>
        <property type="gene ID" value="AFR_41065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8312"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_282117_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007280">
        <property type="entry name" value="Peptidase_C_arc/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04151">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666257" evidence="7">
        <location>
            <begin position="31"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="8">
        <location>
            <begin position="172"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="PPC" evidence="9">
        <location>
            <begin position="922"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ46467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1069" mass="111004" checksum="9F58E47EDC69E5BB" modified="2014-01-22" version="1">MAVGAAALSGPAPATAAPVQAPAPAPVAEAATPKDAPADALDSHDADLLAKAESSGARNVTLIISTDSGKAGDVAGQVEKLGGTVTQRYDKVGYVLASVPTANVVKAAKLAGVSAVDLDEVIQLPELETFRGAAKTSKASGPGAKTPAANPYMPTDEIGSVAFKKKHPSWDGRGVTIGIMDSGVDLDHPALKTTTTGERKIVDWVTATDPVTEGDGSWRAMATEVTGPTFTYMNQTWKAPAGTWKVNRFTEAITLNDEALGDVNRDGDTTDVWGILYNPVTHDIRVDVNQNNDFTDDAVMRPYKEKFDVGHFGTDNPATPVVERMPFVVEYREDVDAKALNLGIVDFVNIGIPEGLHGTHVAGITAANDMLGNRNFDGQAPGAKIVSSRACSWGGGCTAAALSTGMIDLVVNRHVDVVNMSIGGLPAQNDGNNARAELYDKLIKDYGVQFFISAGNSGPGVNTVGDPSVATDVVSVAASVSKETWLANYGSVVAKKNALFNFSSRGPREDGGFKPNLTAPGSAISTINTWLIDPGVAEAGYTLPIGYAMENGTSMASPQAAGGAALLLSAAKATDRGVTPAQLRRALYTSADKIRDTSVAGQGNGLMDVNGAWSLLSRKVETRTYKSSAPVCTAFSDGFATPGRGVGIHNRCAPGEGGLKAGQTKTYTLKLTRTTGPKGSVSHKLSVVGDKKAFKVQSSVKLPLNKTVSVPVRVTPKSGLNSAIVEVDDPRTSVTDFEVLNTVVTGVQPKKPAYEIDAAGTVERNSTTSYFVTVPEGATALQVDLSGIATGSQTRWIAINPWGVPVESTSSLACYTNFSDAAACKPQERAYENPMPGIWELEVESRRTSPMLRNPYELTAKVQGVTVEPATIELPSVTAGVATPVTWTVKNNFGPLELTATGGALGSSLSQRPTIANGASQKYTVEVPVGAERLDVAIGNVSDAAADLDLSVFLGDEQVGQSADGDSEESVSIPTPAAGTYTVVVDGYAVPSGSTQFDYRDVYLSPVLGSMTVPSAPFTLANGASAPVTGSVVAASAPVEGRTLFGDMSLVTDEGAVVGRGTVTIGEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC29</accession>
    <name>U5WC29_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Signal peptidase I</fullName>
            <ecNumber evidence="3 5">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_36300</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the peptidase S26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="3 5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561852.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36300"/>
    <dbReference type="MEROPS" id="S26.025"/>
    <dbReference type="EnsemblBacteria" id="AGZ45516">
        <property type="protein sequence ID" value="AGZ45516"/>
        <property type="gene ID" value="AFR_36300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7346"/>
    <dbReference type="eggNOG" id="COG0681">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028723_0_0_11"/>
    <dbReference type="OrthoDB" id="1741894at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S26" evidence="6">
        <location>
            <begin position="16"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="216" mass="24238" checksum="5EA9195E7069CCA7" modified="2014-01-22" version="1">MIDEQAEEKRKGSFWRELPILLGVAILVAVLVRAFVLQTFYIPSPSMEHTLNVWDRVLVNKLVYDFRDPRRGEIVVFKAPTDWQSGTEGEDFIKRIIGVGGDHLVCCDDQERLIVNGHSLDEPYIYKDADGKQDPAADEKFDITVPAGRLWVMGDHRSASGDSLEHWEQTEDIQAATIESESIVGRAFTIFWPIGRATWLSVPKGFDGVPNAAPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC38</accession>
    <name>U5WC38_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46537">
        <property type="protein sequence ID" value="AGZ46537"/>
        <property type="gene ID" value="AFR_41415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41415"/>
    <dbReference type="HOGENOM" id="CLU_1801907_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="15252" checksum="7671EC174BA3F803" modified="2014-01-22" version="1">MACGPGCRSDLFADFLAQVTDLGRDPVLDVHAVQDGVVTDQHGGADQDDADGDEQDREADGVVAAADQHDDGGDRGQADAGPDQGREEAGGVRRLGVPVRHGGRCYREWRPSRNGQTPGTVSRCPASGMYQQVISRRRGRRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCB2</accession>
    <name>U5WCB2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycogen synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561892.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36500"/>
    <dbReference type="EnsemblBacteria" id="AGZ45556">
        <property type="protein sequence ID" value="AGZ45556"/>
        <property type="gene ID" value="AFR_36500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7385"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_32_0_11"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022622">
        <property type="entry name" value="DUF3492"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11997">
        <property type="entry name" value="DUF3492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3492" evidence="1">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="497" mass="52316" checksum="B591929A56082253" modified="2014-01-22" version="1">MRICLLTGGGYPFRRDALGGWCRTLVDGLGRFTFDLLTITDREPPGGPVFPLPGHVVSAKAVALTRDTRSRQTGGDHATAAASLLCRGLLSEPEPDLADALFAKGLVGLSVLTASAEHPLNGVQLTDVLLDAWRAGRVSAQEERLPRLSLRDARTAATLLRHATRALSVPVPAVDLVHCVGGTTPLLAALAGRWRNGTPLLLTEARTPVARSRAGEERLSPAVRTVLRRFRRAVARTGYAEAGLIAPLSAYHHSWALRHGAEPGRLVPVPAGVDPADFPSAPESRDQPAIVWAGNGGPESGLRPLLDAFAVVAAAVPGTVLHLVGVTAAHEDHCAEQIERTGLGRAVRLHPLPADPRDRYANGHLVVHVPGPSDPPYRLIEAMMSGRAVVGVDVGPVAETLGDAGVVVPADDPSALATACVGLLSAPARRRALGDAARRRALACFTADRVVRVYGALYTDLAGPPPAPAYELALAVPAPRVAQPATLRWLAEAREDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD98</accession>
    <name>U5WD98_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562329.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38705"/>
    <dbReference type="EnsemblBacteria" id="AGZ45995">
        <property type="protein sequence ID" value="AGZ45995"/>
        <property type="gene ID" value="AFR_38705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7832"/>
    <dbReference type="eggNOG" id="COG1514">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094015_2_0_11"/>
    <dbReference type="OrthoDB" id="1696971at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="18503" checksum="15F866803C48CEBA" modified="2014-01-22" version="1">MRTVELVLDPDLDDVVRGVWERLRQAGLRSLATHTHVTNRPHLTLVAADALTPAAEIVPALPLEAELDGLIFLGKAVAWRVVLTDELRDLHATVWHALDGTRRNPLHAPEAWVPHISLALNAPDHAAYDAELAGLGVARGRFAAARSYDSVTRTVTTLRAGPPDPRPAPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEV9</accession>
    <name>U5WEV9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein piccolo</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_41160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46486">
        <property type="protein sequence ID" value="AGZ46486"/>
        <property type="gene ID" value="AFR_41160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8331"/>
    <dbReference type="eggNOG" id="COG1714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079635_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="7">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="19099" checksum="4C030DA01BF7AA6F" modified="2014-01-22" version="1">MLILNVLVNGWLAYQWWLEVEPVFRATMADPFATPDPVSARSSYLILTMLVVATALWFAYEVPALANSGQTLGKRLLQVKVVKLENTDQLGFGRAFRRWARLGMWTPLWSCYGLGFVAQLVDSGSVLFDQKLRQALHDKSAATVVVAVPPGRPEQPAQATTPDHADDSTGGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF97</accession>
    <name>U5WF97_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562913.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41635"/>
    <dbReference type="EnsemblBacteria" id="AGZ46581">
        <property type="protein sequence ID" value="AGZ46581"/>
        <property type="gene ID" value="AFR_41635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8426"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_2_0_11"/>
    <dbReference type="OrthoDB" id="733552at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="28"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28403" checksum="9F773382DC95E4C5" modified="2014-01-22" version="1">MGAAEVEELPGWVTLLRAPNPGPMTLDGTNTWVLRAPGAEHAVVIDPGPLDEDHLARIAGHGPYLFILVTHGHHDHVEGAARLSEILGGVHVLTADPQHSVGAGVLARDESFGGDGLEIRVLDTPGHTADSVCFLVECGDERVVFTGDTILGRGTTVVAWPDGDLGDYLTSLEALTAYEKVVMLPGHGPALDDCGAVARAYLAHRRDRLAQVRAAVAAGADSPETVVDVVYPDIAPGVRFAAEWSARAQLEYLRRESQRTPEQLDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFE9</accession>
    <name>U5WFE9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562972.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41935"/>
    <dbReference type="EnsemblBacteria" id="AGZ46641">
        <property type="protein sequence ID" value="AGZ46641"/>
        <property type="gene ID" value="AFR_41935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8485"/>
    <dbReference type="eggNOG" id="COG4805">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018914_3_0_11"/>
    <dbReference type="OrthoDB" id="520320at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010281">
        <property type="entry name" value="DUF885"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33361">
        <property type="entry name" value="PTHR33361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05960">
        <property type="entry name" value="DUF885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="556" mass="61998" checksum="A5A1F0EFA010090E" modified="2014-01-22" version="1">MGQIDDLANRYVDEWTPLNPAGATFIGVPDHDDKLDDLSPEGFEAHAELTRRTLAELDPVEPETERENVAKDAMMERLGLELARLDAGDVASGLNVISSPVHELRMVFDLMPTQGEQAVANISARLAAFPHALDQYRRTLLDQAAKGNVSASVQMVEVAEQCAAWTDPNRDNFFHRLADGLTADGALATELQRNAAAATAATAGFGDFLRTELAPLGREKQAAGEELYSRASQYFLGARVDQAETYAWGFEELARLEREMRIVAAEIAGPGASVDQAVAVLDADPARRIPGKEAFRDWMQALADKAVSELNGTHFDIPDPVRAIECCLSPTSDGAIYYTAPSEDFSRPGRMWWAVPEGQTEFSTWREVTTVYHEGVPGHHLQIGQTIYRAELLNRWQRLLCFCSGHSEGWALYAERLMDDLGYLADPGDRLGMLDGQALRASRVIVDIGMHLELEIPRDNPFGFHPGERWTPELGWEFLRAHTRMDEEVLRFEWKRYLGWPGQAPSYKIGERIWLQARDDARARQGADFDLKTFHRTALDLGALGLDPLRKALDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNH1</accession>
    <name>U5VNH1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PASTA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_00225</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357278.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38334">
        <property type="protein sequence ID" value="AGZ38334"/>
        <property type="gene ID" value="AFR_00225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.46"/>
    <dbReference type="eggNOG" id="COG2815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092033_0_0_11"/>
    <dbReference type="OrthoDB" id="2035718at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03793">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00740">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="3">
        <location>
            <begin position="190"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="29639" checksum="A9772D42D32EEF40" modified="2014-01-22" version="1">MSDDREPRRQDETNEYFPFGEEGENRPNDGPRKEDTFGDRTEQMPRAPFPDDDSTSVLPPATDDGTRTLSDYRSTDNWADDGDPVWSGRAGVRPQRPGYGDEETDWATVPADEPRGKWWMPIVVGIVGLILLGLLGWGIYLIAQSSGDTTDTTPAVTAPAAPPPAATEPTTTPPSTAPTTTEPTSSPAPSPTDVTVPALRGLSLDEARGALDRKGLSYRLRYVTSESPAGTVIDSDPAEGQQVPADTVVALIIAAAPTTPATPTETPATTDPGEQPEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNT1</accession>
    <name>U5VNT1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative GDSL-like lipase/acylhydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00840"/>
    <dbReference type="EnsemblBacteria" id="AGZ38457">
        <property type="protein sequence ID" value="AGZ38457"/>
        <property type="gene ID" value="AFR_00840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.168"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_805695_0_0_11"/>
    <dbReference type="OrthoDB" id="1247435at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665162" evidence="1">
        <location>
            <begin position="29"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH_hydro" evidence="2">
        <location>
            <begin position="55"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="337" mass="36702" checksum="78D42D31B60F252A" modified="2014-01-22" version="1">MGARRLGIKAVTGLLAIGLIAGTGTSEAAKADPAATVKADDAKAATFTALRVMPLGDSITLGTGGPNRSSYRMDLAQRLLKGGLQINYVGSQASGTGTDIQHEGHGGWSIDELSEELDGWLSQSRPDVVLLHAGTNNLKQGDGPYATAGKLSALIDQIRAARPEARIFVAKIISARAPRELANGRTYNRLIPKLVAEKNDPLITLVDQSSVGGIDLHDLRHPNEFGYSKMAWNWYRAMSRVYGTSGDTGANPYRARTAYRCLGFKAVVDGETHHRTECRTWKLRTATVEIDGVKRRVRAWLTLRSVQKSYRVRVDGKLVKRTRTIEKWTGPGNLLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPG3</accession>
    <name>U5VPG3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357645.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02065"/>
    <dbReference type="EnsemblBacteria" id="AGZ38702">
        <property type="protein sequence ID" value="AGZ38702"/>
        <property type="gene ID" value="AFR_02065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.418"/>
    <dbReference type="eggNOG" id="COG3285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008325_1_2_11"/>
    <dbReference type="OrthoDB" id="2061599at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04863">
        <property type="entry name" value="MtLigD_Pol_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014145">
        <property type="entry name" value="LigD_pol_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033649">
        <property type="entry name" value="MtLigD_Pol-like"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02778">
        <property type="entry name" value="ligD_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="32489" checksum="9B4B81B440D35D73" modified="2014-01-22" version="1">MAPSRVPVQIEGRDLELSNLDKVMYPAAGFTKGEVIDYYTRIAPVLLPHLQGRALTRIRYPNGVDGAHFFEKNAPGGTPSWVRLETLPVPGSTKSRETIDFVVVDELATLVWVANLASLELHTPQWRIGEDPDLMVVDLDPGAPAGLTECCGVAVLMRDRLAEDGITAYPKTSGKKGMQLCCPISGEQDAEVVSGYARKVAEELAAMVPGSITAKMARAIRPGKVFIDWSQNAAAKTTVAPYSLRALESPSASTPLTWDEVEAFATGAAQARQFGAAELLERVEEHGDLLEDLLEPGPRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPG7</accession>
    <name>U5VPG7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02870</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357806.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02870"/>
    <dbReference type="EnsemblBacteria" id="AGZ38863">
        <property type="protein sequence ID" value="AGZ38863"/>
        <property type="gene ID" value="AFR_02870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.577"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_3_11"/>
    <dbReference type="OrthoDB" id="1619224at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="89"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="310" mass="34062" checksum="B596A7A5D0D40806" modified="2014-01-22" version="1">MTDVITRADEHTEAPPPVPTKGGAKDRWARRAPLLPALVFAIIVTQVPFLVTIYFSTQSWNALRPGSRKFVGFDNYITVLTDDRLRSALLNTVILTAGAVIFSMILGLALAILLDRKFLGRSIVRTMLITPFLIMPVAAALLWKHGIYNPDYGLINGIFGGSTAWATSYPKTAIITTLVWQWTPFMMLILLAGLQSQSNDVLEAARVDGAGAWRIFTRMTLPHLRQYLELGALLGSIYLVNTFDAIYTITQGGPGTATTNLPYEIYQTIFSKYEYGEASAAGVVVVILTIIVATFALRVISGLFKVEEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPK0</accession>
    <name>U5VPK0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phosphodiesterase/nucleotide pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357836.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03020"/>
    <dbReference type="EnsemblBacteria" id="AGZ38893">
        <property type="protein sequence ID" value="AGZ38893"/>
        <property type="gene ID" value="AFR_03020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.607"/>
    <dbReference type="eggNOG" id="COG1524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014010_0_0_11"/>
    <dbReference type="OrthoDB" id="191097at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="702" mass="75947" checksum="FF1AB0E4E7475733" modified="2014-01-22" version="1">MPVPATRNRRQELRALFAWPRVIDRTRAVIRSAATTFVVLTFTLWLMPGVRSTDVIDTMGLVVLVAAVGAVLRPLLLAGVVALGGWGAMLLGVVAQVIVMAVALELDPSKRLSGWPTVGVAALLAVVCAAILDWMFDSGTEDTFVKEARRLMRGIRRRQARQAGGRLVTLRRPQAGTEPGLLMIQLDGVSEPVLRWAVRAGNLPTIGRWLRAGTHVMRGWHTGLPSTTPASQAGILHGASRQIPGFRWFEKETGRLMVSNRPRDAAMIEPRLSDGRGLLRDGGVSIGNAFSGDAAISLLTVSHAALPGRSARGWAAFMASPFGFTRALVLGVGEVITELHQARLQRRRNLQPRVSRSGAFLALRPASMLLRDVNVSLIAEQMARGAPAVYCDLVDYDEVAHHAGPARPESMRQLESLDRMLGVLERLAPEAARRYRIVILSDHGQSQGATFRQRHGETLEQVVERFSQPDQAEAPAAPAETEATQDPDFPSSPVAPLLVVSSGNLALIYLTRFPERLGRDRIDHEYPQLIAGLAGHPGVGLVIVDDNGPVAVGAAGRHRLADGTVEGVDPLLPYGPHARADLLRHQQAAHIGDLVLISSVDPVTDEVAAFEELVGSHGGLGGWQTEAMLVHPAEWQITHGDLEGPDAVHRQLVEWLDRLGLRTQTPVEDELEAGGHLAVEQFTQATREHDGAAARLSSSRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ17</accession>
    <name>U5VQ17_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Daunorubicin resistance ABC transporter ATPase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_03050</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357842.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03050"/>
    <dbReference type="EnsemblBacteria" id="AGZ38899">
        <property type="protein sequence ID" value="AGZ38899"/>
        <property type="gene ID" value="AFR_03050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.614"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="329" mass="34383" checksum="E71CF5FC1D56F022" modified="2014-01-22" version="1">MTADLVIEAEGLVKHFGSTKALQGVDLAVPRGTVLGVLGPNGAGKTTAVRILSTLLTPDAGSARISGFDVVRDAEKVRGVIGLTGQYASVDEDLTGRENLVLFGTLLDLGRAGARQRAAELLEWFDLTDAAGRPAKTYSGGMRRRLDLAASLVGSPDVIFLDEPTTGLDPAKREDMWDVVRTLVAGGSTVLLTTQYLEEADALADAITVIDHGRVIAHDTPEGLKRIVGGQTLEVRPSDPAQLALTASILSEVSSGAAADEIRKGMLAVPVTDDRAMTEAVGRLAAAGVAVTELALHLPSLDEVFFSLTGRTASDDDTTSTTIKEEVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ61</accession>
    <name>U5VQ61_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357998.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03820"/>
    <dbReference type="EnsemblBacteria" id="AGZ39053">
        <property type="protein sequence ID" value="AGZ39053"/>
        <property type="gene ID" value="AFR_03820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.771"/>
    <dbReference type="HOGENOM" id="CLU_791378_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665954" evidence="1">
        <location>
            <begin position="23"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="350" mass="36756" checksum="907F092AC6E35F37" modified="2014-01-22" version="1">MLVRLFAVLLAAVAGLPAPAAAAPPSSPTSASAWPYPKACLETGVTRLMAWRNAEPNTIDVVNLVGFSRPCADNPAPVKGVRLALTQYNVTDGVVHGWMGSPWQTNSIGDMTWRRTGTISGDLAAMCLSTGLTGDVAENAYCFRPVWTEDQSEWDVETVPLDDPIVTSPLERIPAPGDSAPAGCGNCLITDPVATLPEPETLPAPGTVVPGCTRLRLDSAGLTSTGTVRLTGSVRACAPDTLETLSVGTVFYGVHGGRLGNTWSLDEGGSVVRFDRAGPLRTGEEAVCVTSGRQQTDDGSYAHHLACVAVETNSRGVTRVVRIPNNDPRVLKPVDVVWNGDPPGTCITCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQM8</accession>
    <name>U5VQM8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable 2-phosphosulfolactate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_04200</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58738"/>
            <dbReference type="EC" id="3.1.3.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ComB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.71" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358074.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04200"/>
    <dbReference type="EnsemblBacteria" id="AGZ39129">
        <property type="protein sequence ID" value="AGZ39129"/>
        <property type="gene ID" value="AFR_04200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.848"/>
    <dbReference type="eggNOG" id="COG2045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070028_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005238">
        <property type="entry name" value="ComB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311">
        <property type="entry name" value="PTHR37311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04029">
        <property type="entry name" value="2-ph_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="SSF142823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="23644" checksum="6C1877CED0542037" modified="2014-01-22" version="1">MTIVGLGDEVPSGAVVVVVDVIRAFTTAAVAFERGVTDIVCVPSVQAGRGLRAQHPDRLLVGESHGLRPADYDFGNSPADLAAAELDGRGLIQATSNGTRGLAQYPDPAALLAVAAVNVGATARWIRQHHPAVPYAIVCTGSTAEDRACARHLSALIDGLEPRREDLVAGIEEGAAQHAAAYARKPAADRVDLSRDLPFCREVDRSDFAMVGEIRDGHVRLLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQW2</accession>
    <name>U5VQW2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4129 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_05370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358894.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05370"/>
    <dbReference type="EnsemblBacteria" id="AGZ39363">
        <property type="protein sequence ID" value="AGZ39363"/>
        <property type="gene ID" value="AFR_05370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1087"/>
    <dbReference type="eggNOG" id="ENOG50331S3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093434_0_0_11"/>
    <dbReference type="OrthoDB" id="1701286at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025403">
        <property type="entry name" value="DUF4129"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13559">
        <property type="entry name" value="DUF4129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4129" evidence="3">
        <location>
            <begin position="150"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24903" checksum="6E00AA6BBF00063A" modified="2014-01-22" version="1">MDLAALRRWWPLAAVLGLLFLASLAATRSAPQLERFNPDAAPTTEPPPLLPPSREVVPPPSAEPVAGGELPDWVGRVALIVLGVLALVVLGLVIWAVLRQTLGRRGTRKGRRDPRRPDSRTAEDLVAALDAGLQELSDTDRDPRRAVIACWVRLEQAAAAAGTPRHAGDSPTDLVARLLAEQHVDAGVLTPFAAVYRQARYATHTVDDQMRQQARTALERLRTDLGAGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRC4</accession>
    <name>U5VRC4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358935.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05575"/>
    <dbReference type="EnsemblBacteria" id="AGZ39404">
        <property type="protein sequence ID" value="AGZ39404"/>
        <property type="gene ID" value="AFR_05575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1131"/>
    <dbReference type="HOGENOM" id="CLU_1346544_0_0_11"/>
    <dbReference type="OrthoDB" id="2083660at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665604" evidence="2">
        <location>
            <begin position="28"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="208" mass="21745" checksum="B3EDE67792ABBA01" modified="2014-01-22" version="1">MHRTARLTMAAATITALTACGTATSSAPTFSSPPSLPPSPSSSPSTAPTTSDPDLVDADLSQLKNYSIDLAVPVVITTSADAGTEYHLDAHPDGRIDFTGTAVTETTRMTVKPAKVRKRTEKTRNTVMIVATPKVAGSAPESCVTDKSEGVLRMDPCRPGDATQSWRLVPAGDSGLFELNGKNTAVRVDEGKLVKEGGYSALQTIAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRE5</accession>
    <name>U5VRE5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00030"/>
    <dbReference type="EnsemblBacteria" id="AGZ38295">
        <property type="protein sequence ID" value="AGZ38295"/>
        <property type="gene ID" value="AFR_00030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6"/>
    <dbReference type="HOGENOM" id="CLU_2128095_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="113" mass="12444" checksum="1B45F255620B29B7" modified="2014-01-22" version="1">MVVGVAESVRKPFTSDDLEVRLRVESVERGTAGDEVQLRTHSQGTACGYEFEEGYRYRVYANGGATTSCAGNERLAFAGREPEGPPQVLWWALGSGATIVAALILLRRRRRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSZ0</accession>
    <name>U5VSZ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_08475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08475"/>
    <dbReference type="EnsemblBacteria" id="AGZ39984">
        <property type="protein sequence ID" value="AGZ39984"/>
        <property type="gene ID" value="AFR_08475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1725"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="784"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="770"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="841"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="864" mass="90454" checksum="CA99AA34CC3C3324" modified="2014-01-22" version="1">MERGGALGDLGRLLADTAGGGRIAVISGEAGAGKSSLATAFAEAAGGTARVLWGACDPLLTPRALGPLHDIARQVGGALREQIAAGERGAVFDALLDALDGPRQLRRPVVVMEDLHWADEATLDLVAFLGRRLALCRVLLVLTYRDDEVGPEHQLRTVLAGLPRPLVRRFPLAPLSAEAVGELARRAGRASSSVYAVTGGNPLLVTEVLAAADEGVPATVSDLVLSRLAALTPAARDAAGFVSVVPSQAEPALLGPRAAGVEECLAGGVLSASAEGVAFRHELLRRAVEESLSPVRRAALHADVLAALAERPGVDPARLVHHAHHAGDAGAVLRWAPVAARRAAEVGANKQATAHYALALRHVAGASVGERATLLEAYATAAYLSGGTAEALDARRQALALREAGGDTVGVGENLRWLSRLSWWNGHTREARVAGEQAVRVLESVAPGPALAMAYSNLSQLHMLAGEATAIEWGERAITLARRFGDLDIELHAMVNVASARLELGIDAGGHELERAHVLAAAAGLDDHATRALVNLASHAVEWCDFDVAAEILDPAVTFAEARDLSGYVRHLLGYRATTLLARGDWAGARADAEAAIAGPRMPGPCRGPAHVSLGRLRSRLGEPGAAEILHEAAEEAYAANELQFVGPVAAGLAEHYWIAGEPGRAAAEARRGYALALEVGQPWFIGQLAYWQWRGGEPVAVPELIGLPYRLVIEGDWRAAAAEWARRGCSYARAEVLACGDNEATAEALHILDGLGAAAAARRVRAELRDRGARVPRGPRPSTAAHPHGLTARQREVLTLLVEGLSNAEIAGRLTLSAKTVDHHVSAVLGKLGVPSRGQAAAAARRLPPTWGELPTRTPGPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT67</accession>
    <name>U5VT67_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative PfkB-family carbohydrate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_09370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359771.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09370"/>
    <dbReference type="EnsemblBacteria" id="AGZ40163">
        <property type="protein sequence ID" value="AGZ40163"/>
        <property type="gene ID" value="AFR_09370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1910"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_5_2_11"/>
    <dbReference type="OrthoDB" id="1604782at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="3">
        <location>
            <begin position="28"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="35019" checksum="7B6EA8617B184201" modified="2014-01-22" version="1">MKIAVTGSIATDHLMHFPGRFAEQLIADQLHKVSLSFLVDDLVVRRGGVASNIAFGMGKLGLRPILVGAVGADFADYRSWLERHGVNCDSVHVSEVAHTARFVCTTDDDLNQIASFYAGAMSEARNIELAPVAERAGGLDLVLVSANDPAAMIRHSQECRARGYAFAADPSQQLARMDGSDVLTLITGADYLMTNDYERSLLETKAGLTSEQVLEQVKVRVTTLGKDGVEITGRGLERIHVPVARDVIAEDPTGVGDGFRAGFFAALGWGVGLERAAQVGSLIAALVLETVGPQEYDIKPDFFLKRLADSYGDEAAAEVQPHLPAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTR2</accession>
    <name>U5VTR2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein sidekick-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_03955</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03955"/>
    <dbReference type="EnsemblBacteria" id="AGZ39080">
        <property type="protein sequence ID" value="AGZ39080"/>
        <property type="gene ID" value="AFR_03955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.799"/>
    <dbReference type="eggNOG" id="COG5184">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_505914_0_0_11"/>
    <dbReference type="OrthoDB" id="308036at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019931">
        <property type="entry name" value="LPXTG_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="SSF50985"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50847">
        <property type="entry name" value="GRAM_POS_ANCHORING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0572">Peptidoglycan-anchor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665691" evidence="7">
        <location>
            <begin position="25"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="392"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="GRAM_POS_ANCHORING" evidence="8">
        <location>
            <begin position="508"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="538" mass="53748" checksum="F6DDB309689EB5FC" modified="2014-01-22" version="1">MCRLGGVVAALFLVLLSMPAAAEAPYPPGISAATIRTATLGAATVAAGGAHSCAVTSLGDLYCWGDDSSGQLGDGREPHGFGTPVRALRGAVQVDAGKAHTCAIDLRGAASCWGDDSAGQLGDGAETDQDSPVKVTGLSGRTLVEITTGARHSCAIDDEGAAWCWGDGSRGQLGAPGVRGSKTPVRVSTGSGMSDPVVDISAGRDATCATTAGGTAFCWGSDAYEQLGTAGHGDRDEPMAVAADGPMRGKIREVGVGGMQACAIDADGLAFCWGTHALGSGTVKPKVEPVAVGVTGTLSTITAGGEHTCGLDGRGQAFCWGEGAGGASRRAVPVGVRTASALRDLDAGDEHSCAFDTRGVVVCWGAGADGQLGTGSTSAAAVPVEVKGLPRPPAAPEGVQVRALDGGLRVFWRPPADMGSGEFLYFWATTADYGSTCTLAVATADGCELVDLENGRQYDVAVVVRTRDGITVSDLVTAAPAAVAPLPSDTPEPRRMAVSVLPGAGGGLPVTGLSPIALTAIGTLLLGGGLAALLVRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUT2</accession>
    <name>U5VUT2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360614.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11575"/>
    <dbReference type="EnsemblBacteria" id="AGZ40604">
        <property type="protein sequence ID" value="AGZ40604"/>
        <property type="gene ID" value="AFR_11575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2359"/>
    <dbReference type="eggNOG" id="COG1413">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_798390_0_0_11"/>
    <dbReference type="OrthoDB" id="2064660at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="38576" checksum="0FE7419C6A427867" modified="2014-01-22" version="1">MLGLFTLVIEILAGVVAATALGIVAVRVFRKVRQRKRAALAAGPRRALLAFVADNGEQGSHDLVAIPDDAWRAAEPGALALLGKLRGEAHLGLVSVFLRRGIARSALRDLHSRSRVKRARAAQVLGNLELRQAVPELCRLLTDRHAEVRVVAVRALGRIGDPAAAWRIIASLDRSDPLPSLLATHTLVQLGAEAEVTLSAALDHPQARVRAVCLDALGLLGATASVHRVARVLKDDAHLDVRVAAANTLGKLGTRTALEPLTRALHASRPPALRAAAARALGDLGAPSAVPTLATLLDDDDFHVAHESAHALRRLGRPGAQALVAAVEAEQARAHADGAGAHARPSASASHAQEALALSRVGVKVEVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUT9</accession>
    <name>U5VUT9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360517.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11290"/>
    <dbReference type="EnsemblBacteria" id="AGZ40547">
        <property type="protein sequence ID" value="AGZ40547"/>
        <property type="gene ID" value="AFR_11290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2300"/>
    <dbReference type="eggNOG" id="COG1988">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071993_0_0_11"/>
    <dbReference type="OrthoDB" id="1667936at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="29026" checksum="93ABE93EE35E029D" modified="2014-01-22" version="1">MMGPSHALSGAAVWLSGSLALDHFAGYEQSPLAVAVGTAVCAGGALLPDLDLSGKVTRNQGGATVARTFGVFSLFVAEVIEKISLGVYTATKLSRDPRRNNGHRTFTHTLPFAGLVGWGTTSLCASQGKWAVVFIVFFMAGLALRGLFDEWAERAGWVIVTLASGVIAWFTAANLPGDRGYPMLGFAVGVGCFVHLLGDMITKNGVPILWPIPMGRRMWRMIGVPNGMAVRVGGKGEVIVLRTLFTLVAVLSVFGLFAPHVLQQLDLDVWARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUY5</accession>
    <name>U5VUY5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alcohol dehydrogenase zinc-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12610"/>
    <dbReference type="EnsemblBacteria" id="AGZ40808">
        <property type="protein sequence ID" value="AGZ40808"/>
        <property type="gene ID" value="AFR_12610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2561"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="6"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="31657" checksum="A13211FCA1903318" modified="2014-01-22" version="1">MVTKFGGPEVLQVIEVPTPEPGPGEVLVKVAGASVNNADVMVRTGINVQYGATGAREQFGLGTDVAGTVAAVGEGVTKFRAGDAVVRTQERLDRPLGTQAEYVLLEPWELAPAPAGVDLVQLATLGLNATTADQALDTLGLKRGDWLLVTGAAGAVGLYAVELARVRGLRVVAQANADDEPLLRAAGAELFVTRDEDLVPTVRRLVPGGVHGAIDAANLAAGAADAVRHGGAFVSLLNAAPMPRREVTMTNMAWHTDPDRLAKLAAFAGAGLISLRVARTFPLEQVVDAHEALAAGGLRGRIVLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV23</accession>
    <name>U5VV23_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360773.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12140"/>
    <dbReference type="EnsemblBacteria" id="AGZ40714">
        <property type="protein sequence ID" value="AGZ40714"/>
        <property type="gene ID" value="AFR_12140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2469"/>
    <dbReference type="eggNOG" id="ENOG50338MW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134413_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045281">
        <property type="term" value="C:succinate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043993">
        <property type="entry name" value="T4SS_pilin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18895">
        <property type="entry name" value="T4SS_pilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000178">
        <property type="entry name" value="SDH_cyt_b560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666110" evidence="2">
        <location>
            <begin position="32"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="112" mass="11336" checksum="92730157543E8F9E" modified="2014-01-22" version="1">MQHIRNRLAGAMVSTAVVLLGFAVLASPAYAADAGTTQLTKILNNATGWLVGILAVLATLFLTLGGVRYVMAGGDPGEIEKAKGAFKSAILGYALAALAPVVVAIVKSILGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV37</accession>
    <name>U5VV37_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyridine nucleotide-disulfide oxidoreductase dimerization subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_12910</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="2">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360927.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12910"/>
    <dbReference type="EnsemblBacteria" id="AGZ40868">
        <property type="protein sequence ID" value="AGZ40868"/>
        <property type="gene ID" value="AFR_12910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2622"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_1_3_11"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="5">
        <location>
            <begin position="6"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="4">
        <location>
            <begin position="358"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="182"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="3">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="473" mass="49023" checksum="F7E73250FFF053CE" modified="2014-01-22" version="1">MTSIEYDVIVIGAGPVGENVADRVVQGGLTAAIVERELVGGECSYWACMPTKALLRSGSALRAARQLPGAREAVTGALDVAAVLGRRDEFASNWQDDGQVSWLESAGIVLHRGQGRLAGDRVVEVTGAGGAVVTLTARHAVVVATGSSALLPDVPGLRESQPWTSREAAAAKEVPGRLVVIGAGVVATEMATAYSTLGSSVTLLARHGVLPAAEPFAGERVTASLREAGVSVRLGTEAVSVKRDQAGAVHIETADGERIEADEVLVAVGRTPNTGDLGLDRVGLAPGAWLAVDDTMRVAGGGQWLYAAGDVNRRALLTHQGKYQARAVGDVIVARAKGEKVDDGSWGRHVATADARAVPQVVFTDPEVASVGLTAAAAEAAGLRIRVVDYNLGAVAGAALHSEGYQGQARMVVDEDGKVIVGLTLVGPDVAELIHAATIAIVGEVPLDRLWHAVPAYPTVSEIWLRLLETYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV70</accession>
    <name>U5VV70_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydantoin utilization protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13085"/>
    <dbReference type="EnsemblBacteria" id="AGZ40903">
        <property type="protein sequence ID" value="AGZ40903"/>
        <property type="gene ID" value="AFR_13085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2657"/>
    <dbReference type="eggNOG" id="COG0145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002157_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="PTHR11365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydant_A_N" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase_A" evidence="1">
        <location>
            <begin position="206"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="692" mass="73566" checksum="77D3B8D98DAFD021" modified="2014-01-22" version="1">MGGTFTDAVASDGADRIVSAKTPTTPPQREIGVLRALEELAHELGIDVPQLLSETDFIAHGTTASINALVQGTVADVGLIATKGHRDSIYIMNAEGRTLGRSAHEIQDTLRQRKPAPLIPKNRALEVTERIDYAGNVLVPLDEDEVRQVARTLVDQGVEAIAVCLLWSFKNPAHEQRIRELIHEIAPELYVTLSSDVSPRIREFARASTTIMNAQIGPRLRSYLTPLQQQLEDGGLQGPLLVMQSEGGTITADRAPEHAITTVGSVLSGGVIGGQRLAAQLGHRNVITTDVGGTTFLAGLIVDGEPVMAPGSIVNQFPINAATIRVHTIGSGGGALASVDSGGNLRLGPQSAEAVPGPACYGNGGTRPTNTDANLVLGILGERGLLGGRKPLRMDLARQAIGEHVAEPLGLTVEEAAIAIYEVQNAQASDLLRRAVVQAGYDPRDFVAYAFGGAGPAHCAGYCQDLGVREVVVPLGPVASAFSAYGLAASDVVVSVELSDPSAFPVGREVLEAHFDRLQAELDRALDRQKVKFSEVTMYREIDLRYSMQVYELAVPVPDGAFTEHTGQEILDRFEQQYERINGSGAGYREAGVQAITYRMRAKASLGFSVVLPTIDAADGPDPAAALLTHRPVCLDSQIGYVPTPVYDYARLRAGHRITGPAVVEVPTTVVVIPAGVLGVVDRLGNLVMSYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVI0</accession>
    <name>U5VVI0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359246.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07130"/>
    <dbReference type="EnsemblBacteria" id="AGZ39715">
        <property type="protein sequence ID" value="AGZ39715"/>
        <property type="gene ID" value="AFR_07130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1455"/>
    <dbReference type="eggNOG" id="COG2329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141544_1_0_11"/>
    <dbReference type="OrthoDB" id="1562645at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="3"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="11019" checksum="B86A0F1414B61A72" modified="2014-01-22" version="1">MAVVKINAIEVPEGAGPQLEQRFAARQGAVENAKGFLSFELLRPVGGETRYFVYTRWETEEDFQAWSAGQGRAAHAGERSRPVASGASLLEFEVVQHVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW25</accession>
    <name>U5VW25_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41178">
        <property type="protein sequence ID" value="AGZ41178"/>
        <property type="gene ID" value="AFR_14480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2939"/>
    <dbReference type="HOGENOM" id="CLU_2379740_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="9575" checksum="5FA7323BA5F2DC3D" modified="2014-01-22" version="1">MDANNPLDEGSEPTAVPGGSIGEQRAGDDPTARDGTDVRPPEKPLAYDPDEEHEPQAGEQGGQPNLSPDPAVAGRRSNFGQGSTDDGPGVPSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW44</accession>
    <name>U5VW44_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_13705</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361086.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13705"/>
    <dbReference type="EnsemblBacteria" id="AGZ41027">
        <property type="protein sequence ID" value="AGZ41027"/>
        <property type="gene ID" value="AFR_13705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2783"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_9_1_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="25762" checksum="D4BF29D7277AFF54" modified="2014-01-22" version="1">MTNEPPTMSRRERLRAQTFQEIEDESFAIIDEEGVHALSIAALARSMAMSAPAVYRYFPSREALVAHLVTRAYEQLVTAMNQAVEGGRRTPRTRVRLLVAAYRDWALQNRRRYGMLFGQQAGLGSGDVSARTPLDQAMAQLVDLLTALPGATPGATATSGDRTLDGQLKVWARTQQRPGTSPRTARAAIVIWTRMHGIVSLELTGIFDNQSIEVQRLIDLEIDNAVQLLASADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWN7</accession>
    <name>U5VWN7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41207">
        <property type="protein sequence ID" value="AGZ41207"/>
        <property type="gene ID" value="AFR_14625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2970"/>
    <dbReference type="eggNOG" id="ENOG5034206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1408067_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="207" mass="21504" checksum="881F1613F4DD0B2A" modified="2014-01-22" version="1">MLTGLLAAPAAIAALDLANPQWSWVEVPASNFVGGRSGWLLSLAAIGAGLASAAMIGPIGARSGGRRAVRWLLGVWATGMIIAGMVPADPPERWDDRSAADTVHGIAALTAFLVLPIAATLLTRAWWRDPPWRRWRALLAVTAAATVAGYAAFLVALVDVMGGPELGIGPYATLVGLLERTMLWSYVGWLACAAVAASRIAPATRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWS2</accession>
    <name>U5VWS2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sensor protein kinase walK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_08960</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359612.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08960"/>
    <dbReference type="EnsemblBacteria" id="AGZ40081">
        <property type="protein sequence ID" value="AGZ40081"/>
        <property type="gene ID" value="AFR_08960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1823"/>
    <dbReference type="eggNOG" id="COG3447">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_410347_0_0_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="312"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="447"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="645"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="678" mass="72378" checksum="A607397E1C538955" modified="2014-01-22" version="1">MTFRQALSRTAGFGLVYLLATYLGRLTVMDGTSLSLVWPAAGVLALWFVAQRTSRWKWLDVAVLAVITMVVNTQTGAPARLAAVFVVANLVQALVFAHLLERWVPGLWSTGGRPLARLSELWGLVGAATVSTTASALIGPTGIWLDSGHYSWTSAAVWLARNTVSILLIGVAARRFDGLLGRDRITKIKVKFRFEYAAVVLCSACAYYFAFLVVDGLPVAFALIGLTAWAALRLHTSFVILHDLAFGTLAVLFTLHGSGPFAQIDSLPMRALVAQLFVGTIAIVGLALALGRDERVALLDRLMASERAATEQAQLLTTIIDSMSEGLGVIDEKGGFLLRNPAATSLLGGRPNPSGQVRDPGYYGLFHADGTPVGEADMPHHLAFGGTGVQNADYLVRNAELPEGRIINLSATRLPVNMDGATHAVVVFRDVTADRRRRDELVSFAGVVAHDLLNPLATIEGWAEALHQSLNERDDHESVDSVIRIQRAAARMSSLINGLLAFTTARDATLSASTVDLREVVGEIAAGRVDQAQSTGTPVPRFVIGDLGAVDADPVLVRQLLDNLIGNAVKYTAPGVTPYVSVVSEPAADGFVRIDVVDNGIGIPPGQHEAIFHNFHRAHRTAGYAGTGLGLTICKRIVERHGGTIAATDNPSGRGSRMTFTLPAGNHVGPEARQRSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWS8</accession>
    <name>U5VWS8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative SirA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359775.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09380"/>
    <dbReference type="EnsemblBacteria" id="AGZ40165">
        <property type="protein sequence ID" value="AGZ40165"/>
        <property type="gene ID" value="AFR_09380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1912"/>
    <dbReference type="eggNOG" id="COG0425">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_4_0_11"/>
    <dbReference type="OrthoDB" id="2042068at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="4"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="70" mass="7837" checksum="AA3445F1E1112863" modified="2014-01-22" version="1">MIEVDSRGRRCPLPIIDLARKMPSVEVGEVVRVLADDPAAANDIPAWCRMKGQEYLGSPAPDTYDVRRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWW3</accession>
    <name>U5VWW3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15410"/>
    <dbReference type="EnsemblBacteria" id="AGZ41364">
        <property type="protein sequence ID" value="AGZ41364"/>
        <property type="gene ID" value="AFR_15410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3129"/>
    <dbReference type="eggNOG" id="COG3324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108054_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="109" mass="11918" checksum="25286EAB8F3D2221" modified="2014-01-22" version="1">MDVHDVERQAAFWSAVLGYRIERGDDGDAHLWPRDGGGLSVWLQPTDAPKAGKNRDHPDLVVADGDLEAEVDRLLALGATRADVGQKGDEGFTVLADPEGNEFCLLHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWY4</accession>
    <name>U5VWY4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361361.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15100"/>
    <dbReference type="EnsemblBacteria" id="AGZ41302">
        <property type="protein sequence ID" value="AGZ41302"/>
        <property type="gene ID" value="AFR_15100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3066"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_11"/>
    <dbReference type="OrthoDB" id="1821409at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="256" mass="26551" checksum="ED8899E1FB889D3E" modified="2014-01-22" version="1">MGIAIVTGGSSGIGRATAVELAKRGEGVIFTYRGNEQAAAGLVAEIEKDGGAAVAVRLDLGDTTTFPAFVDEVRNALSSTWETDRFTSLVNNAGVGGGMTFAETTEEYFDMMSRVLFRGPFFLTQKLLPLLADGGAIVNVGSSSARSNGTERGGGYAAYAANKGAVAVLTRYLAVELAGRGIRVNTISPGTTRTGIGDNAFETYAHLIPEFGKKVLLGRIGESEDVAAAIAVLVSADGRWITGQDIEVSGGYQLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXG0</accession>
    <name>U5VXG0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative cytochrome oxidase assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_10655</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360281.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10655"/>
    <dbReference type="EnsemblBacteria" id="AGZ40420">
        <property type="protein sequence ID" value="AGZ40420"/>
        <property type="gene ID" value="AFR_10655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2172"/>
    <dbReference type="eggNOG" id="COG1612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060266_1_0_11"/>
    <dbReference type="OrthoDB" id="1135592at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="33388" checksum="AAE1051AB769F269" modified="2014-01-22" version="1">MPGVSNRTLLRPLALTSLIANIALVVTGAAVRLTKSGLGCPTWPRCTDESYTTTPEMGINGVIEFGNRVLGIVLGLIALAALVAALLEKPRRRSLIILALAAGLGIPGQGVIGGITVLTDLNPWVVGLHFLLSMTLIALTYALWKRTREGDRPRRTLVPTPLPTLARLTALISAAVIVVGVIVTGSGPHAGDAGARRNGLDPELVSQAHADLVFLLIGLSVALWYALRAVKAPRAAIRAAGILVVVELAQGLIGFIQYFTHLPVILVAAHMLGACLVWVATLNVLWSLRERPFHEARPAPAPVALEPTEPATPTPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXG7</accession>
    <name>U5VXG7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transketolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361801.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16925"/>
    <dbReference type="EnsemblBacteria" id="AGZ41663">
        <property type="protein sequence ID" value="AGZ41663"/>
        <property type="gene ID" value="AFR_16925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3434"/>
    <dbReference type="eggNOG" id="COG0022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_0_11"/>
    <dbReference type="OrthoDB" id="900125at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="11"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="333" mass="35726" checksum="A5ACDD64058CD847" modified="2014-01-22" version="1">MTATATEHVKLTMAQALNRALRDSLAADPTVVVFGEDVGALGGVFRITDGLTAEFGEDRCFDTPLAESGIVGVAVGMAMNGLRPVVEMQFDAFAYPAFEQIVSHVAKMRNRTRGRVTLPMVIRIPYAGGIGGVEHHCDSSEAYYAHTPGLHVVAPATPADAYALLREAIASPDPVVFLEPKKLYWSKEEVELAAPVPGIGRAVVRREGTDATLIAYGPTVPVALEAAAVAAQEGRSLQVVDLRSIVPFDDETVCAAVRSTGRAVVVAESSGFASVSSEIVARVTERCFHSLHAPIRRVTGFDIPYPPPKLEHFQLPGVDRVLDAVDDLQWEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXX8</accession>
    <name>U5VXX8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360481.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11185"/>
    <dbReference type="EnsemblBacteria" id="AGZ40526">
        <property type="protein sequence ID" value="AGZ40526"/>
        <property type="gene ID" value="AFR_11185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2278"/>
    <dbReference type="eggNOG" id="COG2182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_10_1_11"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665326" evidence="1">
        <location>
            <begin position="36"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="435" mass="45865" checksum="73FEDCB79D295CA6" modified="2014-01-22" version="1">MKRRIAVAAALAATLVGAAACGSSSDSSETPNANASVNGAGKTIKVWLMVDAQSGWPEVVKQANDRFTQATGAQVQVDYQQWTDHLAKLDTTLAGNDVPDVVELGNTEMPKYVFNQAFAPLDKGQFENSGQWLTGLSAPCELDGKTYCVPYYAGARVLLYRTDLFKDAGLSAPKTYEDVVAAAKALKAKNGNDKQFSAFLMPGKYWYAAMAWVHGSGGEIAKKDGDKWVGSLSEPAAQEGLTKWADLAKTYSSGDPTKDEADQDPVFAQGKTGMIYANGWELGAVQEQLKDPNDPASAKVKTKVNGKVASAPMPGVPSFLGGSNVAVTAKSPNADLAAKWAQIFTDAKSQEGLIAKGVLPNATSLLDKAAAVKGNEATAQAAKETWFVPMAPKWSDVESANVLQNMLVSIATGKSSVADATKEADAKINEILNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXY4</accession>
    <name>U5VXY4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361962.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17330"/>
    <dbReference type="EnsemblBacteria" id="AGZ41744">
        <property type="protein sequence ID" value="AGZ41744"/>
        <property type="gene ID" value="AFR_17330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3514"/>
    <dbReference type="eggNOG" id="ENOG5031N3X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121458_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666196" evidence="2">
        <location>
            <begin position="26"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="18874" checksum="627B813EEA07D3BA" modified="2014-01-22" version="1">MTRGQKLWLMAVPMMLVPVLGGCGAAEDRPRVASAGRDGSAPASAPPADDTQKAREFVKCMRAAGIELPDPRSDGMIAVPAQAAGSEGSPGAKKMDAAMEKCRDLLPAGGEPPKLSAEDLTKARDLSRCMRENGVPEFPDPDPETGAFAMTEDLGDNSKLGKATEKCRGVGPDALPGITAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYA9</accession>
    <name>U5VYA9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transglutaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_12040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12040"/>
    <dbReference type="EnsemblBacteria" id="AGZ40695">
        <property type="protein sequence ID" value="AGZ40695"/>
        <property type="gene ID" value="AFR_12040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2451"/>
    <dbReference type="eggNOG" id="COG1305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012121_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021878">
        <property type="entry name" value="TgpA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11992">
        <property type="entry name" value="TgpA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="587"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="TGc" evidence="3">
        <location>
            <begin position="468"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="670"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="710"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="910" mass="92952" checksum="4FDE759E82E9C1EA" modified="2014-01-22" version="1">MVRWVRAAVVPVALVVLIALSGVVLGRIYADGFLLQVVAGAALGSVAVSVAARRLPSWSIAPLSVVLLAGYTLGALKLAADRADVAGSLPAIVADALANGIPRLLTAMIPVESTPDTVVVPVVAAWLAGLAGAEVAVRSRRVLLGCVPAAALYCGALYVVGPNADSAGWPTVLFAGLVVLVLAVSARPGGASLDDVPASTRTAVRGRALAGAAAGLVILLGLIVAVGPWVSARVSATPVDPRKYVEPPQVDSLDESPLNRISGWALAPEQSLLEVGPAASGKGTTRLRLAVLPDYDGVTWRVGAVYRNAGRVLPAAPLLPDVPVQEIRQEITVDELAGRLLPVVPTPTGVQGARVAYDPETGTLICPEVLRPGLRYTVTSQSQEPDYNLLPTADSPTGDAVARFLRIGAGVPESIERLSQQLADGNAGSYDRAAAIEEFLSEHYRKVPDAPSGHAYPNLQFFLFGPRDQGGQRGTSEQFAASFALLARLTGLPTRVVVGFDAPRAGGTVTGADAIAWPEVLFDQLGWVAFDPLPKSNQTPRPVEEDFTPKPSPPPTSEPPPASETPSASAPPALAQAQVPQAGGPSAVLVAGGASGSVLLILAAAAGTIVAMRRGLRRRRLEVGTPPERITGAWLEFTDALRLDGRPVPPHLAASEAAAFAATPAVRGKAARLARGQGSGDTGSMDSGKPRNRWLGRKNPADDVLETDPRITNGTGSVPAGPTNTNPHVPGDEARAAGGARAAGGATAASGPAAASGPAMAGASAAGIPMAASEPAATGGMAVDAAAGGPMMAGTPAADRSSATAAAGPTAAGRIASDMAAWGPQQGLSDSAPVRAAAPPPGYTMPPLDELVGAVNTSAFAPGAVDEQQAERAGSQAIAYAEALRAKRSWWRRLWWSLHPGPLRWHRDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYB2</accession>
    <name>U5VYB2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362008.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41767">
        <property type="protein sequence ID" value="AGZ41767"/>
        <property type="gene ID" value="AFR_17445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3537"/>
    <dbReference type="eggNOG" id="ENOG502ZCAV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1487656_0_0_11"/>
    <dbReference type="OrthoDB" id="1363050at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="19030" checksum="0F83CB49761FD2C0" modified="2014-01-22" version="1">MTADLWGTDRQRQNEAYAASMAETVPGVPWDELVAHLADKDNHNRAIAAQLLCNHAAQDPTGRIIGADLDALVAVTWDERFVTARHCLQSLWKVGLGSDAARAAVVTALARRYRDSTSEKNGTLVRNDIVESLRKLHASMADPVIESTARELIDEEPDLKYRRKYARHWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYG6</accession>
    <name>U5VYG6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360814.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12345"/>
    <dbReference type="EnsemblBacteria" id="AGZ40755">
        <property type="protein sequence ID" value="AGZ40755"/>
        <property type="gene ID" value="AFR_12345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2506"/>
    <dbReference type="HOGENOM" id="CLU_2646301_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="76" mass="7567" checksum="061D32A9C6E3B5DE" modified="2014-01-22" version="1">MIEQSPATQSCALGGAAVPEAGLRTLTPARMTGGAAEKGILFEGRISLAAALGATLLSVAGNASVRLPEQETASWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYT8</accession>
    <name>U5VYT8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360895.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12750"/>
    <dbReference type="EnsemblBacteria" id="AGZ40836">
        <property type="protein sequence ID" value="AGZ40836"/>
        <property type="gene ID" value="AFR_12750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2590"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_17_0_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20350" checksum="499F3919671276C2" modified="2014-01-22" version="1">MTTSRIRKDAARNWQHIVDTARRFVDEGEDLRLNDVARAASIGVATVYRHFPTSEALLETLARPRLEGLVAFADRAATGDDAWEGFAGFLLAGVEAQVTDASLPPVLATDKHALPETTELVTRLFSATGQLLDHARAAGAVRSGVTHEDIVRLMCGVAFAAGVHALPDERAALTRRYLNITLTGIRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYU5</accession>
    <name>U5VYU5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inner-membrane translocator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_13070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13070"/>
    <dbReference type="EnsemblBacteria" id="AGZ40900">
        <property type="protein sequence ID" value="AGZ40900"/>
        <property type="gene ID" value="AFR_13070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2654"/>
    <dbReference type="eggNOG" id="COG1172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028880_4_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="34274" checksum="6F8A43EF62D340FE" modified="2014-01-22" version="1">MTTISPPAPRSAWHGLRDALAFRNISAIYIFVVMVVLFSILAPDTFPTVDTVRIILTDQALTAILAIGLVAPLAAGAFDLSIGSQLGLAAIVVAWLLAHQDFGLWTAIAATLAVGVVVGLINGLLIVKAGISSFITTLGMSSVVLAAIAWISDDAQILDLGSDFQQIATYEIFGISTSVYLMLVLGVVVWYVLDNTHLGRVVYATGGNAGAARLSGVRTDVVVVASMVACAVLAAVAGILLSSSLATGDPTVGPGYMLPAFAAVFLGSTQFRNGRFNVLGTVVAVYVLATGVKGLQLAGAPVWIPELFNGVALLLAVAITRLRKSSRRPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYX4</accession>
    <name>U5VYX4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360935.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12950"/>
    <dbReference type="EnsemblBacteria" id="AGZ40876">
        <property type="protein sequence ID" value="AGZ40876"/>
        <property type="gene ID" value="AFR_12950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2630"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_2_0_11"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665886" evidence="1">
        <location>
            <begin position="30"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="43"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="27540" checksum="F624AD8C4FC98A58" modified="2014-01-22" version="1">MRITFSRRAAVVGATTLALLGAAAVPAMAMSTGKPAPSPKPTIVLVHGAWADSSSWNPVMQRLRHDGYQVRAIANPLQGLTSDTAYVTSYLATIKGPKVLVGHSYGGAVITNAATAVPDVKSLVYIAGFIPAKGETIGELAAKSTPALPLVSTQVPGGTEVTIDPAQFRTAFAGDLDKDAAANLAAAQRPANTNAVTDPSAVEGFRSIPSWDLLTRKDHAINPELQRFMATRAHARITEVDASHAVMLSRPDAVTKIIEQAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZY3</accession>
    <name>U5VZY3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20300"/>
    <dbReference type="EnsemblBacteria" id="AGZ42332">
        <property type="protein sequence ID" value="AGZ42332"/>
        <property type="gene ID" value="AFR_20300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4116"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069607_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="29712" checksum="5827B6212CB09D94" modified="2014-01-22" version="1">MAEVQAAPVRRFSSRFLRSELRLIAVRRRNQVGLAALAAIPIVLAVAMKVAAPDREGRGGGGPDFFAQITGNGFFVALAALGVEIGLFLPIAIAMLAGDAVAGEANQGTLRYLLTVPVGRIRLLAVKYAGIVAGAFLAPLVVAVSGLLIGLILFGGGDVTLLSGTQVGFGEALLRLLGVCLFLGVCLSALGAVGLFLSTLTEQPMGATIALTMLTVASFVLGQIPQLDWLHPYLLTRHWQDFGELLRDPVSFSALTPGLLSALAYIVVFLTAAWARFAGRDVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W007</accession>
    <name>U5W007_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_14820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14820"/>
    <dbReference type="EnsemblBacteria" id="AGZ41246">
        <property type="protein sequence ID" value="AGZ41246"/>
        <property type="gene ID" value="AFR_14820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3009"/>
    <dbReference type="HOGENOM" id="CLU_2968864_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="58" mass="6710" checksum="7C06A7C125560085" modified="2014-01-22" version="1">MAYSIRCADFNNDCPAQFSTPDEDELLQHVELHTKVVHQPMEWTPELVEVVKTHITKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W089</accession>
    <name>U5W089_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Catalase-peroxidase</fullName>
            <shortName evidence="8">CP</shortName>
            <ecNumber evidence="8 9">1.11.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peroxidase/catalase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">katG</name>
        <name evidence="12" type="ORF">AFR_15270</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>2 H2O2 = 2 H2O + O2</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>AH2 + H2O2 = A + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30275"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="8">Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer or homotetramer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8">Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the peroxidase family. Peroxidase/catalase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.21" evidence="8 9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361395.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15270"/>
    <dbReference type="EnsemblBacteria" id="AGZ41336">
        <property type="protein sequence ID" value="AGZ41336"/>
        <property type="gene ID" value="AFR_15270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3101"/>
    <dbReference type="eggNOG" id="COG0376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025424_2_0_11"/>
    <dbReference type="OrthoDB" id="49441at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01961">
        <property type="entry name" value="Catal_peroxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000763">
        <property type="entry name" value="Catalase_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30555">
        <property type="entry name" value="PTHR30555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00460">
        <property type="entry name" value="BPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="SSF48113"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00198">
        <property type="entry name" value="cat_per_HPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="7 8 9" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEROXIDASE_4" evidence="11">
        <location>
            <begin position="161"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="cross-link" description="Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with Trp-127)" evidence="8">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ41336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="757" mass="82725" checksum="A627FED6C4159728" modified="2014-01-22" version="1">MSDTQDNPASAQGVDKKAEAGCPVAHDSVTAHGSESENPAIDSPTPKTGGRPHTNRDWWPNQLDLSVLHAHSDKGNPLGPDFSYAKEFAKLDVEAVKRDITTVLTTSQDWWPADFGHYGGLMIRMSWHAAGTYRIQDGRGGAGDGGQRFAPLNSWPDNANLDKARRLLWPVKQKYGQSISWADLLVLGGNVALESMGFKTFGFGFGRVDVWEPEEIFWGPEDAWLGDERYVSDDKMAEDVGATEMGLIYVNPEGPRGSGDFRAAAHFIRETFARMAMNDEETVALIAGGHTFGKTHGAGPADGHVGPEPEGAEIDKQGLGWLSTYGTGKGPDTITSGLEVTWTDKPTQWSNRFFEILFGYEWELSTSPGGAKQWVAKDADAIIPDAYDAAKKHKPTMLTTDLSLRFDPTYEKISRRFLENPDEFALAFAKAWYKLLHRDMGPVSRFLGPWVAEPQLWQDPVPAVSGALVGDADVAALKAKVLESGLTTAQLITTAWSSAASFRSTDKRGGANGARIRLEPQRSWEVNQPEQLATVLTALEGIQQEFNAAGGAQISLADLIVLAGSAAVEKAARDAGVEVTVPFRAGRTDATQEQTDVESFKVLEPRAEAFRNYLRPGEKTQPEVLLIDRAYMLDLTAPEMTVLIGGLRSLGANVGGVQHGVFTDRPGVLTNDFFTNLLSPDTRWKASESEEHVYEIRDLATDKVKWTATAVDLIFGSNSQLRALAEVYASEDARGKFVADFVKAWTKVMELDRFDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0B4</accession>
    <name>U5W0B4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21915"/>
    <dbReference type="EnsemblBacteria" id="AGZ42653">
        <property type="protein sequence ID" value="AGZ42653"/>
        <property type="gene ID" value="AFR_21915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4443"/>
    <dbReference type="eggNOG" id="ENOG502ZC50">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097508_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23412" checksum="BD13ADF790FD56F1" modified="2014-01-22" version="1">MTAHDLAAALPDIAALRDRCRALAVLDAVLDPGSDLPYYGYDAAWGPGVELASMDNGSGDNWSIVFAKTGALLRGFDHESPMSPAVNDDVWPGVIDTVPAVFAEHLEEPAFQFDGSFAATVCLWRQAGDDRWHAGPISFPEGADPDGARWMFRMLTEPAPAAYLRFAQDYYEREIDAAAVADVYALRPLTDGTVRRLNPEVSLAELAPDLAAIGYPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0F4</accession>
    <name>U5W0F4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363069.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22140"/>
    <dbReference type="EnsemblBacteria" id="AGZ42698">
        <property type="protein sequence ID" value="AGZ42698"/>
        <property type="gene ID" value="AFR_22140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4488"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_11"/>
    <dbReference type="OrthoDB" id="1186478at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="27649" checksum="1A283EE87FA72413" modified="2014-01-22" version="1">MTAVDYRNQTTLITGASSGIGTEFARRIAARGSDVVLVARRRDRLEKLAAELDVKATVIPLDLSRPDAGQALSEEVADRGLRITSLINNAGFGTHGPFHGEDPQRLTEELNVNIASLVGISRAFIEPLRSAGTGVLVNVASLAAYQSSPNMAVYAATKAFVLSFTEALWGEARGTGLRVLALSPGLTRTEFFDVVGTDVMAQRATFQTPGEVADFGLRVLDRRNPPPSAVSGRLNRVLSTGSRLLTRRRTVVVSGMLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Z0</accession>
    <name>U5W0Z0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363053.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22060"/>
    <dbReference type="EnsemblBacteria" id="AGZ42682">
        <property type="protein sequence ID" value="AGZ42682"/>
        <property type="gene ID" value="AFR_22060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4471"/>
    <dbReference type="eggNOG" id="COG3595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081211_1_0_11"/>
    <dbReference type="OrthoDB" id="1059710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="29006" checksum="E8C742179072BA6B" modified="2014-01-22" version="1">MPTFETPRPIEATLELVVGDARVTAGDQARTVVEVRPSNPGTEADVRTAEQTRVEFSDGRLLVKTPKTLRGGIGLFGKTGSVDVEVSLPTGSTLRGDAAVAAFHCTGTLGDTRIKTATGDVRLDRTGALAVTTSGGAIVASSVDGTADLKTATGLISVGDITGGAVVKNSNGETRLGAVGGDLRAKNSNGDIVVGQARAGVDAATANGSVRIGAVHRGAVSLRTAAGSLEVGIAVGTSAYLDLHTQFGKVLNELESTGAPEAGAPSVEINARTSFGNIVVHRAPAGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1J0</accession>
    <name>U5W1J0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative RNA polymerase sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_17515</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17515"/>
    <dbReference type="EnsemblBacteria" id="AGZ41781">
        <property type="protein sequence ID" value="AGZ41781"/>
        <property type="gene ID" value="AFR_17515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3550"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014322">
        <property type="entry name" value="RNA_pol_sigma-B/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02980">
        <property type="entry name" value="SigBFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="19"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r3" evidence="5">
        <location>
            <begin position="98"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="7">
        <location>
            <begin position="183"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="25837" checksum="62BE8207C1130FC7" modified="2014-01-22" version="1">MAALPVDHPSRPAVRERAIEAWLPLARHLANRYSGRGEPNDDLLQTAVVGLIKAVDKFDPDYGTDFAGYAIPTVIGEIKRHFRDRTWAIRVPRRLQELRLAITAANSTLTHTLGRSPTVPDIAAHLDITEEEVLEGLEGARAYNATSLSTPVGSEGSSELGETLGGEDHGYELAEARIALGPAMASLDEREQRILTLRFYGNLTQTEIAEQIGISQMHVSRLISRALVKLRGQLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1K7</accession>
    <name>U5W1K7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24075"/>
    <dbReference type="EnsemblBacteria" id="AGZ43083">
        <property type="protein sequence ID" value="AGZ43083"/>
        <property type="gene ID" value="AFR_24075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4877"/>
    <dbReference type="eggNOG" id="ENOG50324Y3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_878882_0_0_11"/>
    <dbReference type="OrthoDB" id="2140603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="34209" checksum="9ECD655E6B3D2206" modified="2014-01-22" version="1">MLEDDLRSTLRDRAAQPAVQPDLLDQVRGGIRRSNRRRAAVAGAAAVIVAAIAVPFAFTEEKSPPPVAPAIGWPAPQLQLPAFPFTPSWVPPGPDTADVGQLGPNLVLSYEEQQIQLEVGPVPGSWEMEGEEDHQVALNGTQATVRTGSYVDSRPGDRYVGIRWRLADGRWVQLNSFGPAKENDMLRFARGLREQPVPASPPPVTVAAAPPGLVVQQLTRENICLAPPAALSERDSGRGICVSVEVTSAEDKPQPEDEKITLNGRPAWISEGDDGPSELTSTLTGKRTLTIYLDREDVPLTREELIRFAEGVAVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1V5</accession>
    <name>U5W1V5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">IclR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_23695</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363597.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23695"/>
    <dbReference type="EnsemblBacteria" id="AGZ43007">
        <property type="protein sequence ID" value="AGZ43007"/>
        <property type="gene ID" value="AFR_23695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4801"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_7_3_11"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="74"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="27854" checksum="50699D64F5446F62" modified="2014-01-22" version="1">MSPAQRDDTGAGAGGLDRAAAILAAFDDTHRELTLAALVARSGLPRSTAHRTADKMIKLGWLDKPHDRYRIGNRLFELSGLAPVRHELREAVLPYLQDLYTATRTTVQLGVLQATQVLVVEKITGHRPMPMLSQVGGMIPAHCSGIGRAILAYSDAATVDTVIEAGLVRRTPRTITSPTALRRELAAIPERGLAVDREEGNIGVSCIAAPIFGPLGQVVAALSVTGPTAVVRVDRAGPAVRLAAAASSRAYSHRRVPLSGTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2D1</accession>
    <name>U5W2D1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363762.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24530"/>
    <dbReference type="EnsemblBacteria" id="AGZ43172">
        <property type="protein sequence ID" value="AGZ43172"/>
        <property type="gene ID" value="AFR_24530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4968"/>
    <dbReference type="eggNOG" id="COG3393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085660_0_0_11"/>
    <dbReference type="OrthoDB" id="1270792at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013653">
        <property type="entry name" value="FR47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08445">
        <property type="entry name" value="FR47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="96"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24485" checksum="BE81D56BE0F5B189" modified="2014-01-22" version="1">MEHLDNPVWAALTGPQAALADRYDRSARFPDEISMFAGLEDPLDPAAWRDLGALAGPGGEVLVAVPALAPPDGWVLTGGASGVQMVGTEVEGAPDPEAVVLTEADIPEILDLVGRTRPGPFRKRTSDLGTYLGIRREGALVAMAGERLRVPGHTEISAVCTDPAFRGAGLAARLIRAVTANVRERGDTPFLHAASVNTGAIRVYERLGFTLRMPIEFAAYQVPAEGVSPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2H1</accession>
    <name>U5W2H1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH-type transcriptional regulator betI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19515"/>
    <dbReference type="EnsemblBacteria" id="AGZ42175">
        <property type="protein sequence ID" value="AGZ42175"/>
        <property type="gene ID" value="AFR_19515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3960"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_23_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="18194" checksum="C7EE9C683EA2C754" modified="2014-01-22" version="1">MAAAAPLFYTQGIRAVGMAQVVEAAGCGKNLLYTHFPAKTDLVAAYLSLVRGERERSTGRAVQGARGPEEALIAVLGEIADRVREPGFRGCAMRNYLTEFPTGDDAPDRIARDYLASTEQQIRDLSRDDTLADRLWLVVESLYAVAGRPDAPARARVAVDLATELVGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2N5</accession>
    <name>U5W2N5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AIRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362543.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19495"/>
    <dbReference type="EnsemblBacteria" id="AGZ42171">
        <property type="protein sequence ID" value="AGZ42171"/>
        <property type="gene ID" value="AFR_19495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3956"/>
    <dbReference type="eggNOG" id="COG1691">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065705_0_0_11"/>
    <dbReference type="OrthoDB" id="1744359at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039476">
        <property type="entry name" value="P2CMN_synthase_LarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064">
        <property type="entry name" value="PTHR43064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="1">
        <location>
            <begin position="90"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="22237" checksum="57310A92E6FEC26A" modified="2014-01-22" version="1">MPDLSSYAELDLDRSARRGYPEAVYCAGKTPEQVAGIAAAVAGRPDVITLFTRASPAHAAAVLEVLPTAFHDDDAGLLAWPPEVPAPTGGPVLVLAAGTSDLPYAREALLTARYLGRDTSLIVDVGVAGLHRVLGHLEVLRRAHAIVVAAGMDGALPSVVAGLVSAPVVALPTSVGYGAAFEGLAALLAMLNACAPGVAVVNIDNGYGAGHLAAQIAAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2R3</accession>
    <name>U5W2R3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EAL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364103.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25775"/>
    <dbReference type="EnsemblBacteria" id="AGZ43419">
        <property type="protein sequence ID" value="AGZ43419"/>
        <property type="gene ID" value="AFR_25775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5225"/>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031868_0_0_11"/>
    <dbReference type="OrthoDB" id="510714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019278">
        <property type="entry name" value="DICT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10069">
        <property type="entry name" value="DICT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EAL" evidence="1">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="418" mass="44211" checksum="CF0D46A92C4AE41D" modified="2014-01-22" version="1">MPDIHEVIDRRSVTPVFQPLIDIASGRVLGYEALSRGPAGTPWESPALLFAAARAVGRDSELDWVCRAAAYRAALDAGLEQTLFVNMEPTAWRTPCPPDLEPVVSLAQRRLRVVTEMTERAIADDPSGLLSATASCRTAGWGVALDDVGADPMSLALMPFVHPDVVKLDMQLLHAPDDPHTSQVVSAVAAYAEASGATVLAEGVETPEHELIALTMGATIGQGWLYGRPGPLPVPALPTGEPLALLGRTAGHTTGTPFEIVAAHRPVRETTKSALMPMSRHLEALARDSHQPPVLLACFQEARNFTPATARRFGRIATRSPLVAALGAGLSEEPTTGVRGADLAAGDILRGEWNVILVGPHQATALVAKDLGGTEPEAQRRFAYATTHDRALVIAAARSLLQWLAPSRAADVSGLLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2R4</accession>
    <name>U5W2R4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25180"/>
    <dbReference type="EnsemblBacteria" id="AGZ43302">
        <property type="protein sequence ID" value="AGZ43302"/>
        <property type="gene ID" value="AFR_25180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5102"/>
    <dbReference type="eggNOG" id="COG0484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006181_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="865" mass="93517" checksum="69576191D4FF2432" modified="2014-01-22" version="1">MDRSGDKRALNAAARQSGDLHLFLPEPGYRFTALRAHPPPAPTRVRRQPSALLAEAHHLVPFLGRDEELRTLLTWRDSPGDYGVALVHGSPGQGKTRLARWFADQSAAPGWLTLEAALIGDGPDPARDPRQLPAGDRGRIIVVDRAERWPVELLRRALADARVHDQRTRIVLLARPAGNWWLRITAYLRRSFDIVPVGVALAPLPPESGDRLFAAARDSFAEVLAVPGARGLSAPEWSAGPTALEVQLAALAAVYATGRHEEITTGPGAVADYLLDREYENWAGRHTGPETLARAVYLAGLAGPLRREQAASLITGIALADDGEECAFLIDDHRSCCPAEGGEALAPLHPGLLAEDFIGLRSGGRRSGTQPDPWADHKLPEIVRSLATAAPDRLVAALTTMVTVAQRWEPFAREQLGPLLRELPEVVLAAGSPVLSALAEVTTLDAATLHDLEAEMPIDGTPDLMDPLATLTRRLYERMRTAQPRDATVAALGYRLASRLVRANRHPEALAPIEHAVAWWTRPERREEPGHHLNRALSCRAEVLDGLGRIDDALDSAEAATALWWDAAGWEPDADFAAHLTLHARLLAERGHSAEAVRHQADAIMIWQASARPDHQLALARALTRQNTYCRAAGAGADAYAAIRRAAARWTKLDTEQPGVHRVELLDALTALAAAAEAPLSIRLDAGERAGKGFWLLWRRDITLLPQTAAALRAWAAALSESGAHDEAFDKVGRARNIAVRLNRQNEKRYARLLAMVQLTYGRCCLESGTRLDEGLAAARTAEEIFRTRRSMTAEAAATRLVADLSQARPGGALERPAPVTTRAARDDSGTCPRCLGSGRQKRGAFGGGKTWSVVCSGCGGSGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Z8</accession>
    <name>U5W2Z8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25640"/>
    <dbReference type="EnsemblBacteria" id="AGZ43392">
        <property type="protein sequence ID" value="AGZ43392"/>
        <property type="gene ID" value="AFR_25640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5197"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038054_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019026">
        <property type="entry name" value="Peptidase_M64_IgA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09471">
        <property type="entry name" value="Peptidase_M64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665644" evidence="1">
        <location>
            <begin position="22"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="32618" checksum="F35CCCF17E520D42" modified="2014-01-22" version="1">MAAAAAVTVVVYGLVQLPASADTRTEVREVFSPDGSIRQESVKVADLPRTSAAPAVAADVVTIQQTGTPATRYDLVFVGDGYTAGEQELFHQQVLARWDQLTAIEPFRTLKDKFNVWQVNAVSNQSGSDNDPTLGVEKDTALDGEFFCAGLDRLVCVDETAATGYASLAPGADQALILVNSSTYGGSGGSVTVSSGGNALSGDIVVHELGHSIGGLADEYGGEGTYEGGELPEPNTSVADEATMRAEQLKWFSYLGKPTPDGGVIGTFEGGSYFDRGVYRPSEDSMMRSLGNEFNLLGIDQLTAAILAETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3A1</accession>
    <name>U5W3A1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MDMPI_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364255.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26175"/>
    <dbReference type="EnsemblBacteria" id="AGZ43497">
        <property type="protein sequence ID" value="AGZ43497"/>
        <property type="gene ID" value="AFR_26175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5305"/>
    <dbReference type="eggNOG" id="ENOG50314EV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094601_0_0_11"/>
    <dbReference type="OrthoDB" id="2054078at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017517">
        <property type="entry name" value="CHP03083_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024344">
        <property type="entry name" value="MDMPI_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11716">
        <property type="entry name" value="MDMPI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03083">
        <property type="entry name" value="TIGR03083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MDMPI_N" evidence="1">
        <location>
            <begin position="12"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="23837" checksum="C287583D8A296D11" modified="2014-01-22" version="1">MDHDEVWKAIDTERAGLADLFESLTPQQWETPSLCEGWRVRDVAAHLTLSQTGLVTALLDTVRARGSLSRMIRDSARRQAVRVPPDRYAALLRAMAGSRRTAPGVTPLEPLIDVLVHGQDIALPLGLRRPMPVQASAAAADRVWPDLWPFRAERRLRGFSFRATDDSWSAGAGPVVEGSIGAILLLLTGRRAALDQLSGPGAEHMRVTGGPGTVQARRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3B2</accession>
    <name>U5W3B2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane-bound lytic murein transglycosylase F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_26225</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364276.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26225"/>
    <dbReference type="EnsemblBacteria" id="AGZ43507">
        <property type="protein sequence ID" value="AGZ43507"/>
        <property type="gene ID" value="AFR_26225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5315"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_18_4_11"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665506" evidence="5">
        <location>
            <begin position="18"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="6">
        <location>
            <begin position="83"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="320" mass="34143" checksum="35B741386B4A132A" modified="2014-01-22" version="1">MRLRAALVAVSTLTVLAATGCAGDSSPLPGRPTPSAEVAPSGQAAPADTSCRPQASLRPAGALPAPGKMPAGSYMETIQKRGRLVLGTSQDTLLFSSRNPFTGKVEGFDVDMGRQIAAAIFGDPDKIQIKVIGYDKRVTSVLDGSVDIVADTMTANCARWKDVNFSTIYYNAGQKVLVSKDSPAKTINDLGGKRVCAAKGSTSYDNIGKVASRTKPIAVDRPGFGDCLVAFQQNEVEAISTDDTILIGLAAQDPYAKVIGDRFTEEPYGMAMPKQHEDFTRFVNAVLARNRSNGTWRSTYERWLGDFGAAPKPPAAEYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3D8</accession>
    <name>U5W3D8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26350"/>
    <dbReference type="EnsemblBacteria" id="AGZ43532">
        <property type="protein sequence ID" value="AGZ43532"/>
        <property type="gene ID" value="AFR_26350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5341"/>
    <dbReference type="eggNOG" id="ENOG5031TDC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188865_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="89" mass="9598" checksum="B622E8EAE2D28767" modified="2014-01-22" version="1">MVKWIVIAVVVLALIVLVASVLSVLGRLSGLERAGRKLERRQQEATKLQQGAVVLEQSVAELQQRAELMQSQLAVIKAGRGASDGKHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3N6</accession>
    <name>U5W3N6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560170.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27860"/>
    <dbReference type="EnsemblBacteria" id="AGZ43833">
        <property type="protein sequence ID" value="AGZ43833"/>
        <property type="gene ID" value="AFR_27860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5646"/>
    <dbReference type="eggNOG" id="ENOG5032VV6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1864006_0_0_11"/>
    <dbReference type="OrthoDB" id="1884228at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15352" checksum="82FA2BCEFA041A23" modified="2014-01-22" version="1">MELHWWSIEIFDSATLSAARWQDAHGNALTEAAITHGAYEWSWRPHSWGVLLEIAFRSDEQWHVFRDLPAVHAALDAVPDPVNGLMVYPGRGGSSGRVQPRRPRPIMGAGAAALPIEPEMLGAVLAESEPRTTRPGLLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3N8</accession>
    <name>U5W3N8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362957.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21575"/>
    <dbReference type="EnsemblBacteria" id="AGZ42585">
        <property type="protein sequence ID" value="AGZ42585"/>
        <property type="gene ID" value="AFR_21575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4375"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="162"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="365" mass="37610" checksum="81A942AA5B5694F7" modified="2014-01-22" version="1">MIGTRAQAVDATAAVLVLAVCGYAAQEAGRASWWQWLLVGAVALPVSVRRRWALPAAAVTLTASAAVLAGGVIAPDASPGLCAAAGLTLYPVGAGLPLVRSVPVLATGLAGAATLGLLWPAAPLVAGPAVAVPWLVGVLVRRRREVAEQVFEERTARAVTEERLRIARDMHDTVAHSLSMIAMKAGVARHVAAVRPEESMAALEVIETAGREALVEMRRAVGLLRADQDPAVPSGDDEDLRTLTRRTEQAGVRVETTLAGETTPPPGIRLVVYRIVQESLTNVVRHARATGCTVSVDAGPHSVTVRVADNGTAAVADGSTGHGLRGMRERVTVYGGTLQAGPQPGGGFAVTASIPYATSGGDGQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3U1</accession>
    <name>U5W3U1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_28135</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560225.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28135"/>
    <dbReference type="EnsemblBacteria" id="AGZ43888">
        <property type="protein sequence ID" value="AGZ43888"/>
        <property type="gene ID" value="AFR_28135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5703"/>
    <dbReference type="eggNOG" id="COG2229">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077970_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="22423" checksum="C45FF0B79C23415A" modified="2014-01-22" version="1">MYGRFSEPAPTPAPLPPVAVKIVISGGFGVGKTTFVGAISEIEPLVTEAEMTERSIGIDDTSAVSAKTTTTVALDFGRITLDDALLLYLFGTPGQDRFAFLWDDLVDGALGAVVLLDVRRIEDCFPAIDYFEEHEIPFLIGVNTFDRSQRFDLEEVRDALGIGPDVPILDCDARHRESVKNVLVALTEEVLTKRLSRERPVPTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3W9</accession>
    <name>U5W3W9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein phosphatase 2C-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27280"/>
    <dbReference type="EnsemblBacteria" id="AGZ43717">
        <property type="protein sequence ID" value="AGZ43717"/>
        <property type="gene ID" value="AFR_27280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5528"/>
    <dbReference type="eggNOG" id="COG0631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034545_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="24860" checksum="84DA967539085BD7" modified="2014-01-22" version="1">MAGRALTVGAGSVIGNRYPANFDVLHVDPSRPLVVVADGMGGGRGSAVAGRTAVDVFVARAAPSPQALRAAVAEVQTRVRAAGAGLGELTGCTLTAFVGAEDEPAAWIVQLGDSRAYRLRGGLLELLTTDHTIAWLGAVYGWWPADSSQAHAARYRLTRYAGHPDAPEPDVLSVTLHPGDVYLLCTDGLAEQVGYDRLAEVLASPDPDRAAETLLGDSLRAGGDDNATAAVIRVGTGATHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W419</accession>
    <name>U5W419_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560105.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27530"/>
    <dbReference type="EnsemblBacteria" id="AGZ43767">
        <property type="protein sequence ID" value="AGZ43767"/>
        <property type="gene ID" value="AFR_27530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5580"/>
    <dbReference type="eggNOG" id="ENOG5032YX4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176213_1_1_11"/>
    <dbReference type="OrthoDB" id="2016098at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="103" mass="10561" checksum="75255061D6D7D562" modified="2014-01-22" version="1">MHNLGFALEGAWKVLLASVILGAGLPLLFALGIRSLAYGAGGDAEVHESGVTGPKPHAIGTVLGYALFAIVLLGVALGITFIVASGFGKALSFEHIYPMIVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4H8</accession>
    <name>U5W4H8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sugar efflux transporter B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_28305</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Set transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560259.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28305"/>
    <dbReference type="EnsemblBacteria" id="AGZ43922">
        <property type="protein sequence ID" value="AGZ43922"/>
        <property type="gene ID" value="AFR_28305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5738"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055598_3_0_11"/>
    <dbReference type="OrthoDB" id="626204at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004750">
        <property type="entry name" value="Sugar_efflux"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23535:SF2">
        <property type="entry name" value="PTHR23535:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="10">
        <location>
            <begin position="193"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="369"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="399"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ43922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="450" mass="45917" checksum="86D07CC71FDB3388" modified="2014-01-22" version="1">MPIFVLFAAVLLLGIADAMVNSYIVLFGADVVGLTPLQIGVWTSVFALGGITISWWLGRLFDRRPARAYAIVVILLGSAGYLLLPRVSNFFVLLLMAATVLGAMGAAFPQLFSLARSVLGEGAAGQRSAPVLRSAWSLAWAVGPLLGALVLSHGGYSWLMWTAAGVFFATALTVLVVPRPGAAIVTDSAPGPTPVLLTVGVTLFFAAMFAGGLALPLFVTRELDQSPTSIGVLFSVCAAVEVVATLGLALVPGRVSQRTVILGGFVSFVGYYALTVVSTGMLMLVMAQVLRGVGIAIVAAAGIRYFQDLLAPATGRATTLFANASTAGLLVSGILSGLAVEHLGYRTTLLLCGVAAALGGLTFALGSRHHPGGTSPDSGQAQLGNGGRDPADQPATMSPDQAGPDNTSPDSGQAQLGDGDRDPADQPATMSPDQAGPDNTSPDKAGSPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4T1</accession>
    <name>U5W4T1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_28780</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560354.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28780"/>
    <dbReference type="EnsemblBacteria" id="AGZ44017">
        <property type="protein sequence ID" value="AGZ44017"/>
        <property type="gene ID" value="AFR_28780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5834"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="8"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="319"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="386"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="462" mass="48714" checksum="4EB9FC99486052DB" modified="2014-01-22" version="1">MRSLAGRYRVEQPVGEGGSAVVHSGYDRTLKRRVAIKLFTPYRPDSPAPSVDVLREARAAAALSHPNIARVYDYGEATEDGKRVPYLVMEFLEGDTVADQLATTGAFDWPRAAEICADVAAALAAAHARDLVHRDVKPRNVMLTPSGTKVLDFGIAAMAGQDGFDSQGQLWGTPASLAPEQLRGEPTYPAADIYALGLLLFECLTGDRAWPGTSVGEILAARHQQRAPRLPSIPGLPRSIVRLYESCVADDPARRPSALEVVQVLRIPALRSSTVITRTIAPVRPKRSRSRAAITASIAVAAAFVSIIGLQVANGYSTPGGREAEAAVDGAPATKAPQPITTPAPKPTSTRVVPISDGDNDRPVRRVTDTPTREATSQVRPVKPKPTPSSAAPSTKPTTRPPGPVPSDPTPTTTEPEPEPTDPPATTEPPAEPTETPPADPDPEDPPPADESVVLAGLSPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4W6</accession>
    <name>U5W4W6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44169">
        <property type="protein sequence ID" value="AGZ44169"/>
        <property type="gene ID" value="AFR_29540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5988"/>
    <dbReference type="eggNOG" id="ENOG5031UE4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716948_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="385" mass="41280" checksum="C4256BD20574E46C" modified="2014-01-22" version="1">MVYRMQDVLRSVEAMPPPPSRTTTDDIIGRARRSQIWRNVALGSGVAACLAVVVTVPALALTGPDMGGAQPAAQPAQQQSSAPPPATPTDPPLPVKKVAFRTDLAGYRIGEFQVGPPAYVTAGYTELPVYRDGVVGLMSTPQGTSPTMLPVEIATITVYAKDVYDTATFGGAGNATLAIGQRYPMSIEGRQAWTRDWVYTSPVNPKKKMTMAALAWQHADGAWATLVPNFNSLDLSREQATKIATGLTTRTPVDLKVPYRLSYLPANWQAVAVRQNPAANRNLISQVFLHQGPVADPAARIDDVLPGNLQISLMKGLGAGKAESIKKEGVHCTPGKATCAVIHGDYRIDLYGYGGTLSDTEIKKIADGLQLGDLAHQNTWYKVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4X9</accession>
    <name>U5W4X9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_29615</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560521.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29615"/>
    <dbReference type="EnsemblBacteria" id="AGZ44184">
        <property type="protein sequence ID" value="AGZ44184"/>
        <property type="gene ID" value="AFR_29615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6003"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005315_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011528">
        <property type="entry name" value="NERD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08378">
        <property type="entry name" value="NERD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50965">
        <property type="entry name" value="NERD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NERD" evidence="3">
        <location>
            <begin position="12"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="212"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="540"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="799"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="895"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="810"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1464" mass="159445" checksum="8EEC00FCA813AF20" modified="2014-01-22" version="1">MQEGRWTTITPSQFAHEREALAYVQQLLPDTEPYRAWSNFTFTAQTGHPYEVDLLVAAPSGLYLIEIKSLNGRLVSSGSNWIQHGPSTTRTFDNPLHLADAKAKRLKSLLQVAASRRRGMPVRIPFVQAAVFLSKPGLQVELSEHHLHGVYGPEPVGTNRPNRLPTIGSLLQRPPQDERQRLTAETSRALPDLLAEVGIARSRRHYQVGSWELETRPFDVGPTWQDHLAKHRDLERERRRVRIYLVERNAVQAERASIERAAKREMLVLHGINHPGIVQADSMESHEAGPALIFRYDPRAMRLDHYMAQFGDRLDAPARLTMIRQLAETVAYAHGRRLHHRALSARSVLVNPGRKRRGGTDEEAWLFPQLQISDWQAATRGADSAAGSDGSGGSVALSSHAASHLERSAEAYLAPEMIAPRPDPVAMDVFGLGALAYLILTGQPPATKRPELLARLARDNGLRAASVADSVSEFADELVQAATAPVPAQRLTTVAEFVEMIEVIEEELTAPSAPSSVHPVDKPEPTDPLEARPGDRVGEWVVRKRLGTGSTCRAFLADNERTGRAEVLKVGLSDEKDNRLRHEARVLGPLTDSRVIRLARPEPVRIADRTIVVLEHAGDLTLARKLRDDGRLTVDELETYSDYLFGALDYLEGEGVYHRDIKPDNIAIRIRPNRTRQLVLFDFSLAGVSVKEITAGTPRYLDPFLGSVNRPVYDKHAEWYALAVTLHEMASGELPDWEDGSVLPEFSDGPPVLAAEAFDPAIRDGLVDFFHLALHRDSRTRFASLKDMRDAWQKVFRRSDASAPVGTDHPEPQEEPDAEGAEAARDEAAAKATRATALEAAGLTPRAVSAAHRLDATTVGDLLTVANKLAHLPGLGAKTRQELQRRVKEWRARLGERETLPTSPNARRSAAAEAATETTGDSADAELVRVGLDAIATLLIPAKNGRNATEVEGTRILLRLPDDTGVLPGLTPWPQQPQVATALGVTPGRVAQILGKQRKRWNDLPVASSVRAELIELLRDGGRVMGVEELATALLTSRGSMRTGDDLRRTVALAAVRAAVEIDALAHEPRLLLRRHGDRLMVALEAAEDDAPDTPAAPALLDYADALGKAADRLAGNEVLPSPATVLRTLGAVTAPGGAADAIDERRRVVLAAVASENAAATARLEIYPRDLDPVRALRLAQAGVLPPAGPPGQPRGLTPAQIADRVAARFPDLAPLPGHPRLDGLLTDAGFELIWNRDHYAAPARTAASSSMTIVRRRSSASTPPTRWTADSPVLASALRAEERLTGAASAGGFRALTVRLSRYALAREELVRRFSARPVNVASRFLSHLHGLVDARPKPTWETVLGADIAEPGSRPAIKLGEYVDEAWSQAAPALLTQLAAGSGPILLHDAAVLARYRAMERLYELADAARAGNGDVWLLCPMDDPDLLPKLDGTVVRVGDNEWIALPDAWVENTHRSASVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5D7</accession>
    <name>U5W5D7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44222">
        <property type="protein sequence ID" value="AGZ44222"/>
        <property type="gene ID" value="AFR_29805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6039"/>
    <dbReference type="HOGENOM" id="CLU_1691743_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="159" mass="17286" checksum="F9F4D8079A279980" modified="2014-01-22" version="1">MTDASVHLAAQPRLDTLVTEFHSDAQRLRTDFAGTAWIGDIPDPASGMMGASYQRRSVDSQLFLPDTHWYTAEELDDAEVQVAVGLLQVPGTTQGTQGLVGAIADPGVDFFEHPEQDDRVGICLTLRGQIWSNVGLSYRITVLCRPESLIRSSTTVTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5E1</accession>
    <name>U5W5E1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44227">
        <property type="protein sequence ID" value="AGZ44227"/>
        <property type="gene ID" value="AFR_29830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29830"/>
    <dbReference type="HOGENOM" id="CLU_1313226_0_0_11"/>
    <dbReference type="OrthoDB" id="1884071at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="23452" checksum="C37E603BF102C8E9" modified="2014-01-22" version="1">MVAAADRRAIEQAWLASLVTRNQIRRSVWPRLTFLRHMPQHGFMPSQYFHPTYCGVCGMRESEEVVTSECLAGDAFWLRPIDTLWAGAAVEQFDGADDDHDVHRGRAVLEDIVDAIRSLPGSAQLTELNAALIGKLKSNKRERTVLLEALGYAGALPTAEYPSYATEFVSYDDANTRMPSQFYKKEWAYPVRFWTGVDGVDPARLPTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5E4</accession>
    <name>U5W5E4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363721.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24325"/>
    <dbReference type="EnsemblBacteria" id="AGZ43131">
        <property type="protein sequence ID" value="AGZ43131"/>
        <property type="gene ID" value="AFR_24325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4927"/>
    <dbReference type="eggNOG" id="ENOG5032YR4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117192_0_0_11"/>
    <dbReference type="OrthoDB" id="1950882at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="15058" checksum="B3CDB16366BE885D" modified="2014-01-22" version="1">MDNTVKAIVAGAAGATALNAVTYLDMAVRARPASSAGEDSVSKLAELAHVPLGEDKSAENRKSGLGPLLGYATSTAAAVAWTAAGGRRMPTGVSAVALTVLGMLGSNAPMTALGVTDPRSWKAADWVSDLVPHLAYGWVTALTLRALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5G0</accession>
    <name>U5W5G0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase, IS891/IS1136/IS1341</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_29930</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29930"/>
    <dbReference type="EnsemblBacteria" id="AGZ44247">
        <property type="protein sequence ID" value="AGZ44247"/>
        <property type="gene ID" value="AFR_29930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6062"/>
    <dbReference type="eggNOG" id="COG0675">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_IS605" evidence="6">
        <location>
            <begin position="153"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="7">
        <location>
            <begin position="288"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="367"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="43903" checksum="947DE0C99C7AF32F" modified="2014-01-22" version="1">MDQRTAESSYGVPGELLTPPLSWSLAGLRKAWNQAKPQVAPWWPEVSKEAFNTGLDALARGLKNWAGSRTGTRAGRPVGFPRFKSRRRTTPSVRFTTGSIRVEPDRMHVVLPRLGRLKLHESARKLARRLDLGTARIMSATVRRDRGRWHVSFTVEVERAEHIPARPASVVGVDVGIKTLAVLSTGELIDNPRHLVAAQQRMRSLARALSRKTGPDRRTGRRPSKRWEQAAARLGGAHTRVSNLRRDGLHKLTTRLTREHHTIVVEDLNVTGMLRNRRLARHIADAGFAEIRRQLAYKTGWNGGRLLIADRWYASSKTCSACGTVKAKLALSEREYHCQACGLIIDRDRNAALNLAALAAQFDTAGSGPVAGRGADQKPRTRGQVATKRQPGTTPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5I0</accession>
    <name>U5W5I0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_24870</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24870"/>
    <dbReference type="EnsemblBacteria" id="AGZ43240">
        <property type="protein sequence ID" value="AGZ43240"/>
        <property type="gene ID" value="AFR_24870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5038"/>
    <dbReference type="eggNOG" id="COG2409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005108_8_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="553"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="594"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="MMPL" evidence="8">
        <location>
            <begin position="55"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="MMPL" evidence="8">
        <location>
            <begin position="469"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="733"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="793"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="841"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="897"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="954"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1045"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1124"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1182"/>
            <end position="1196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1274" mass="126647" checksum="10DC88E8B0A7AAC0" modified="2014-01-22" version="1">MRNAFLEEIVVFAAWGSWVARFRWPVLVLTVAAVAAAGVWGLGVFGQLSEGGYNDPGSESTRAANVVRDELGAQGGDVVVIYTPTSGGIDDAVLGQRIENTLGALPGSAVTAVASYWPDKVAQYKSADGSSAVAVLTLAGEDDGEKLESYREIDDKLAVEGATVQAAGGAPLGDASATRSTQDLAFAEAISLPIVLVLLLFIFGSLVAASLPVLVGGCAVLGSLGVLHAVAYTHDVNSFAVNVASLLGLGMAIDYGLFMVGRFREEQGFGRTPAQAVTRTVATAGRTVVFSATLLMIALAGLLLFPQGFLKSLAYGGLAAVALAAILSLTLLPALLAVLGPRVDKLPVRLPRRKNAAAPGTGWSRLAGAVLRRPVLVALPILAGLLVLAAPIRGVEFGENDERILPAGDSARMAIEALKSDFPALSSAGIQVVLRGTKGEPPPAAVGKAFTDAAKKVPGIAAVTPAGTGGDAVVLTATLAGKDPFSPVARDAVDALRALPVTGDTEVLVGGATARNVDSLTATADRLPLMVSLLVGATLILMFLAFGSVLLPIKAVVMSALSLSATFGILVWIFQDGHGADLLQVTPAPLEVGIVVLMAAVVFGLSTDYEVFLLSRMVEARTRGASTAEAVTTGLARTGRVISAAAVLLIVVTGAFALSSVTTMRFVGVGMIIALFLDATVVRMLLVPAVLKLMGEAAWWSPGPLRRLQQKAGLAEYEDEELFPTVSVGRHAAPESAAAPARTSSASAPRHASRAETGPGALPAPPPSAAYAGESFGAAASPFAGDDPDSTGPVSFAGSSPSFSEDAPDTTVPLSERGSGSPDQPFAGASPDETRSGTAAEWAGDNPSETVTGTGSAAEGHDAPDTSAFGGVTPVSPSATGSAPVSPAAAGFGSGAGSSPVSRSATGSAPVSPSATGSAPVSGSAYGPGPIAGRGHGLPVAGSRPHPAPSKITLDESPASTSGGWPTSSASRSDETPADEAGSAAQSSGPPFVTGHASGGASSSDAFAGSSEAAAGRTTNRPSGEPPTTPAAGAPDAGPFTAPESADRTSGTSPAGSDSSGGRSPAGYPTGSASPSRPHDRPGSGTPAGSPSARDRQAAGPAAGSPGGSSVAPSMWPPTGVATRATGPSPTAASTGPGTPGTGVRGAADSENSAVTASAPGWPATPGSASDDPPTLTGWPDATDTGERSQAGPTGNSPGADPGDSRSVVDPMDTRPTANLADHLTGSGAQGRPGDPPADSAAAGKTDEEPGPETTAVLELRPTVDLADRRDQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5S6</accession>
    <name>U5W5S6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative ADP-ribosylglycohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31170"/>
    <dbReference type="EnsemblBacteria" id="AGZ44494">
        <property type="protein sequence ID" value="AGZ44494"/>
        <property type="gene ID" value="AFR_31170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6309"/>
    <dbReference type="eggNOG" id="COG1397">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024566_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="SSF101478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="319" mass="32473" checksum="727A6A224E8DDD54" modified="2014-01-22" version="1">MTKKASGMLRGPMTFTLFPESRLALATDSLAGLSVGDALGAQYFVPGNAAADLARGDLPTPPWPWTDDTEEACCLVAVLTGSDELSFDRDRFADLLSEQFDAYRGYGPGAVVMLRQIREGTPWPIAAAAAFDGQGSAGNGAAMRAAPLGAWHADSLAHAAAQAVRAAEVTHAHPEGIAGGVAVAVAAAMAAAARLDGHRPPAGQLLAAVAGLVTPGHVQDGLRRAVRIKDAAEAAYELGTGSRAMAQDSVPFAIWVAAQHLDNYPAAITACVAGGGDVDTTAAMAGGIVAAYTGVDGIPAAWLAAREPLPKWVAASREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5U2</accession>
    <name>U5W5U2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363996.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25505"/>
    <dbReference type="EnsemblBacteria" id="AGZ43365">
        <property type="protein sequence ID" value="AGZ43365"/>
        <property type="gene ID" value="AFR_25505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5168"/>
    <dbReference type="eggNOG" id="COG5267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026001_0_0_11"/>
    <dbReference type="OrthoDB" id="637794at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014917">
        <property type="entry name" value="DUF1800"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08811">
        <property type="entry name" value="DUF1800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665578" evidence="1">
        <location>
            <begin position="41"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="514" mass="54219" checksum="6979F95702A9E575" modified="2014-01-22" version="1">MKTKPEPTRRAVLGGAVAAAATVAGGLTLDAALAVPAAAATSVPTDLVSADPLAHLLRRATFGPTPAALAEITKLGAASWLDRQLDPAKIDDSALDSLLTRLPLAGADIATVRAKLPVHSYDAFGQLGRAAVARAIWSNRQLFESTAAFWANHLHIAAPSSGVWDSRPDYDRTVTRKHTFGRFADMLKAAARHPAMLTYLDNRSSTKTLPNENYARELMELHTVGMIYSEADVQAAAKLLTGLTVNSTGTYAYTAAKHATGAVRILGFSHANSTAEGGEAAALAFLDHLSKHPATAERLATKLCHRFVADDAPATLVAKLAQVYLDNDTAIVPVLRALFTSPEFAASIGQKVRTPFEDLVASVRTLGLGPEASGVKALDALYNNLVNAGNAPFRWSPPNGFPDFAAAWASPSAFVMRCNNHLNLATGWYPKQLTRPTDMLKALVPVLPATYGGLVNALATRLIGTTLPADHVAAVLSVAGKLPTSSLTSSDKSIAGSLPYLIALVLDSPSFQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6W3</accession>
    <name>U5W6W3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33425</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561280.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33425"/>
    <dbReference type="EnsemblBacteria" id="AGZ44943">
        <property type="protein sequence ID" value="AGZ44943"/>
        <property type="gene ID" value="AFR_33425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6765"/>
    <dbReference type="eggNOG" id="COG2215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030955_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666145" evidence="3">
        <location>
            <begin position="26"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="641"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="669"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="716"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="743" mass="78460" checksum="C7D7DFC5456EE28A" modified="2014-01-22" version="1">MIRKLLAAGAIALTGALLLPAPAQAHPLGNFTTNQYAGLRISGTGVDVDYLLDLAELPAYQAQHEEIDTDRDGKQSRPERDAYAERTCAAAAGATEITIAGRRVALDARPAGLTFPPGTGGLATLRLQCVLHADARIESRTEIGYRGNLFPDRIGWREVTAVGDRYTLDTPAVPADSVSARLTAYPTGMATSDVRSVVLDVRPGGPAAAPAPFPVSAGTQARDVDRFTAWFTDLVGRPELTLGVGVLAFLIAVVLGGAHAVAPGHGKTIMAAYLVGSRGRLRQALTVAVTVAATHTVGVLALGIVLATSVQLAPDAMYRWLQLASGVLVALVGGHLLLRALKNMRARQEMHDHDHDHGGDGLDRPLAVSKRLPTGEGSEHDHNHVHAHAHSEPVSERLPTLVGAEHEHHHDHNHPHEHRLPHPVSERLPTLVGAEHEHHHDHDHPHEHQLPHPVSERLPTRDEAEPEHQHHHAHPHAVPVPALVGKHSLTPAGVGHSDSLPVGERLLTAGAAAEPDHGHDLGHGHGHGHSHGHGPSSSPSHGHGWGPSHGHDHGPSSSHNHDHDHDHDHDPSSSHNHDHDHDHDPSSSHDHDHSPSPDHGHSQGQAVATASRVTAHVHSHGGSSHTHYIPAPDEPLRLRTLLAMGFAGGLSPSPSAVVVLLGAAALGRAWYGVVLVAAYGIGLAATLAGIGLVLARWGERLHRVTAGRWSHLLTRRLPAVTATIILAVGVGMAAVATLGLLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W714</accession>
    <name>U5W714_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32670"/>
    <dbReference type="EnsemblBacteria" id="AGZ44792">
        <property type="protein sequence ID" value="AGZ44792"/>
        <property type="gene ID" value="AFR_32670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6611"/>
    <dbReference type="eggNOG" id="COG4762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085646_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025450">
        <property type="entry name" value="YndJ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14158">
        <property type="entry name" value="YndJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="28505" checksum="400770ABA63C0062" modified="2014-01-22" version="1">MLVNLLVAVGMLVVVPAGLRLLADPAAEVARRFWPVGAIAGAVSLWLPRGPVATALATIYAIGAALLCAYAVSRPIRPRREWPAEAAVLTALISPAIAASALIAERAGYHLFGFELGVLVLTVAHFHFAGFAAALIAGLQTRIAPGRAADAAALTVPAGTLLVFVGYFTAEAVELAGALVLTAGMWTVGWLIWRYSRTSVPDRLTATLLGVSAAVLAVSMVLAVSWALGEATGLPHPSLAWMAATHGVANALGFAVCGMVGWRRAGAYLAPGRNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W734</accession>
    <name>U5W734_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alkanesulfonate monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561150.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32770"/>
    <dbReference type="EnsemblBacteria" id="AGZ44812">
        <property type="protein sequence ID" value="AGZ44812"/>
        <property type="gene ID" value="AFR_32770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6632"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_1_4_11"/>
    <dbReference type="OrthoDB" id="919913at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="366" mass="39158" checksum="A622F0ADC6B4A4B8" modified="2014-01-22" version="1">MTVEFVGMIGTRDVSETRAPSGPPIDLEYVRRFSRVHEEAGFDRVLIGYGSSTPDGTQVAAYAAAHTSQLGLLIAHRPGFVAPTTAARTFATLDQLSGGRVAVHTITGGSDAEQRRDGDYLGKDDRYARTEEYLSVLRRAWTSPEPFSHDGRFYKFEDFSLLAQPAHRIPLYFGGSSEAAYRAGGAVADVYALWGEPLKETAEQIAKVQASAAAAGRDPLGISVSFRPILAATDALAWERAERILATIEESKGGKGSFTGDKTRVLGGPSPENVGSQRLLAAAARSDRHDRALWTATAKASGAQGNSTALVGSPETVAQALLDYVDIGVTTILIRGYDPVDDAVDYGRHLLPLVKQELSHRSLVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7J0</accession>
    <name>U5W7J0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hsp90 ATPase activator family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28520"/>
    <dbReference type="EnsemblBacteria" id="AGZ43965">
        <property type="protein sequence ID" value="AGZ43965"/>
        <property type="gene ID" value="AFR_28520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5782"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_6_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="162" mass="18480" checksum="2758CAC9AB0FCB63" modified="2014-01-22" version="1">MSELHIEMPPDEPVIAFRRLVKAPPDLVFRLYTEPEHLRHWWGPRHLILTTCDIDLRVGGRYRLVQRAPDGEEFGFHGTYLAIERPHRLVKTFVYEGRPDNEATDTFTFEPVGDDTLVRCRTVHSSVAARNAHAQAGASPGLTASYQRQAEHLATFRKGPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Q6</accession>
    <name>U5W7Q6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560362.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28820"/>
    <dbReference type="EnsemblBacteria" id="AGZ44025">
        <property type="protein sequence ID" value="AGZ44025"/>
        <property type="gene ID" value="AFR_28820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5842"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051674_1_1_11"/>
    <dbReference type="OrthoDB" id="1828250at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="24550" checksum="4374B431921098FF" modified="2014-01-22" version="1">MKITPGGVVAAEWTKFSSLRSTWITTGISVVLLIAFGVIASASISDDSLSAVDLALSGSSLAALTVGVLGALLGAGEYTTGMIRATLAAVPRRLPVLWSKSLVAGFAALITMTAGAFAAFALGSAVLNDGVSGVGLGDAGVVRALFGAGLYLGLVAVLGVALGMLVRSSAGAIAILAGLLLIVPGLAALLPDSISETITPYLTSNAGSAVMALTPGEGTLGPWTGLAVFAGYVAVALAATAYRLKKTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7X9</accession>
    <name>U5W7X9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_29045</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560407.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29045"/>
    <dbReference type="EnsemblBacteria" id="AGZ44070">
        <property type="protein sequence ID" value="AGZ44070"/>
        <property type="gene ID" value="AFR_29045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5885"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_11"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="229"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="286"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="544" mass="55806" checksum="3FCF80E0CE0EEC05" modified="2014-01-22" version="1">MSETRIADGQVATLRRRRSFVDLSVNVKILVAVTMAAVVALAVGIVGLMALSRASAAAQLIYQSNLASVDVLGRLQAAMVQTRLDLANHAISRDEGSKAKYRAAFTADLQAVTAEIAAYRGTDPAGDPTTIADLESVWQKYADIAQNQQLAASESGDLTGWERTRDTAIAPLLTEINQHLKELAAAERSSAASTAGSARSDYRTSRLVFITLLVVGLLTALGLGVWVARQIVRALQKVKSVCDSLAAGDLTRSAGLTSRDEPGQMAAALDTAVAKFRETVTTIGGSAATLSAASEELSAVSTQLQSGANDAATQATSASAATEQVNAGVQTIAAGAEQMSASISEIATNASRAAEIARQGLVVAERTNGQVAELGVASSEIGDVVKLITSIAEQTNLLALNATIEAARAGELGKGFAVVAGEVKELAQQTAKATEEITSRITNIQVSSGSAAEAIGEITSVIGQIGDYTTTIASAVEEQTATTSEMSRSVAEAANGSGEVARTVSGVAEVAGATADGAKATQQAATDLTRLASDLTTVVNGFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Z9</accession>
    <name>U5W7Z9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_35090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561610.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35090"/>
    <dbReference type="EnsemblBacteria" id="AGZ45274">
        <property type="protein sequence ID" value="AGZ45274"/>
        <property type="gene ID" value="AFR_35090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7100"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="148"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="222" mass="23889" checksum="713F00D2127CE03F" modified="2014-01-22" version="1">MTVRVVVADDQSLVRTGFRMILTAGGVDVVAEAATGAEAIDAVRRTRPDVVLMDVRMPELDGLEATRRILTGAAAAPRIIILTTFDLDQYVYAALAAGASGFLLKDVTPEHLVAAVLMVQNGDALLAPAITRRLVQRFARRDPETATLHRDLATLTPRETEVLRLLARGLSNAELAATLHLSEATVKTHVARILAKLNLRDRVQAVVVAYETGLVTPTTPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W831</accession>
    <name>U5W831_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35240"/>
    <dbReference type="EnsemblBacteria" id="AGZ45304">
        <property type="protein sequence ID" value="AGZ45304"/>
        <property type="gene ID" value="AFR_35240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7130"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_609212_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="443" mass="47210" checksum="BF95FEAB8887C44E" modified="2014-01-22" version="1">MIVALIELSLDSPAPPEAATPPPASFYRRAGLALTVVLVLALGGAAPVSSMLWSRLGGVPLPAGGDFQVTTDRIFTMDLDASPRVLTAWQSDPVRRLWSIEATGSGDPFLVRDATADLVLVQQGSSISVLDGRTGNLRWTSDVPVQRLTGTVGVVRKETFRPGTEYDPESGDPGRLYGVFGDSLHTEPALSTELRGVELATGRPLWSFKVPGSAFTAWPDEPGSAFAVLSAQKLTLISAETGRVVRERTVPRIDGQGPAMGEVVDNMVMVHYGAFGAGGKVVAYALDTLDERWHQDQPDPSGNPAACYGVSCTVTRDNLVVLDVDTGEPKWQASGPDILSFGPASVLEVRNLTTPVRSVSLGTGQTVADLDGWRDYYQVRGEPAVLLSRPQDTRTTAFGVLRAGRTAVQPLGVLPANVLQCQAAPGTVVCQTFEQLEVWSYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W847</accession>
    <name>U5W847_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative fatty acid desaturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561519.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34630"/>
    <dbReference type="EnsemblBacteria" id="AGZ45182">
        <property type="protein sequence ID" value="AGZ45182"/>
        <property type="gene ID" value="AFR_34630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7006"/>
    <dbReference type="eggNOG" id="COG3239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016265_1_0_11"/>
    <dbReference type="OrthoDB" id="1032884at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="PTHR19353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="FA_desaturase" evidence="2">
        <location>
            <begin position="60"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="344" mass="38148" checksum="68762B25F1C13B60" modified="2014-01-22" version="1">MTTTTERPRSTTGSDFAALNKRVTKAGLLERKPAYYAVRLTAVSLMLIGGWTAFFLIGSSWWTLAVAAFLAVTFAQVALVAHDLAHRQVFRTKRPGEIAGRIAGNVAVGMSYGWWMDKHTRHHNNPNHDDLDPDVTPEVLIWATESALGRRGLKGFITRHQAGLFFPLLTLLGIDLKVSSVKALIRGDMKRRNLEAGLLIMHAVVYLTTLFVILSPLQAIAFLVVHQALFGVYLGMTFAPNHKGMPHPDGTEDYLRKQVLTSRNVTGGWLTDAALGGLNYQIEHHLFPAMPTPNLRKVQPIVEAYCAEIGVPYESTSLIESYRQALRHLNEVGAPLREQHKAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W883</accession>
    <name>U5W883_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_35835</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561759.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35835"/>
    <dbReference type="EnsemblBacteria" id="AGZ45423">
        <property type="protein sequence ID" value="AGZ45423"/>
        <property type="gene ID" value="AFR_35835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7253"/>
    <dbReference type="eggNOG" id="COG3979">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030648_2_1_11"/>
    <dbReference type="OrthoDB" id="1110723at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004236">
        <property type="entry name" value="Pept_S1_alpha_lytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001316">
        <property type="entry name" value="Pept_S1A_streptogrisin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035070">
        <property type="entry name" value="Streptogrisin_prodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02983">
        <property type="entry name" value="Pro_Al_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001134">
        <property type="entry name" value="Streptogrisin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00861">
        <property type="entry name" value="ALYTICPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 11" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665798" evidence="8">
        <location>
            <begin position="29"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Pro_Al_protease" evidence="10">
        <location>
            <begin position="125"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="202"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="389"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ45423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="440" mass="44232" checksum="49456F9B6393C988" modified="2014-01-22" version="1">MQRRTVVAIAATVTAAGAAVAFTLPSMAGTSPSRRSAPQATGAVAPQLLAAMKRDLGVSTDQAEARVKRAAWASGVAAQLRSASGDSYAGSWLGKDGTTLNIGITDAGLAGQVRAAGAVPKLVERSVTELDGAKKALDAEATKADRDLPGWYVDVAANKVVVQALPGDTDHARELAEDAGLPAAAVKVVVTKKAPRTFADVIGAEPYFVNIGGGQARCSIGFAVEGGFVTAGHCGAEGTPTTDRENLDQGEVVASVFPGTADMGVVETNAGVTLQPFVNDFDGNALPVGGSTEAPVGAAVCRSGSTTGTFCGTILAKNQTVNYPEGAVTGLTRTDVCAEGGDSGGPWLSGDQAQGVTSGGSGDCTVGGETFFQPITEILEVNNLTLLTTGSGSGGGAEASTPPAEATTPPADEEATPPPAEDDDAGQQRGGHHRRGHHRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W925</accession>
    <name>U5W925_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562028.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37195"/>
    <dbReference type="EnsemblBacteria" id="AGZ45693">
        <property type="protein sequence ID" value="AGZ45693"/>
        <property type="gene ID" value="AFR_37195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7525"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_19_5_11"/>
    <dbReference type="OrthoDB" id="504558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="5"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="387" mass="40466" checksum="61A9300F84749150" modified="2014-01-22" version="1">MTKTAVVAGGGIAGTVVALALHRAGFTPLIHEAHDRGADERGAFVTVAENGLSALEALGLDPRQVFSAGFATPAMALLTAAGKPLAELPLGTTTTTIRRADLYLALRAEAVRRGITIAYGKRLTGRTRRDGGVTAHFEDGSAVNADLLVGADGLHSRTRTALDPAAPAPRYLGLLNAGGFTAGPVAPALAPEPGVMQMAFGRKAFFGWATAPDGSVWWFANPPSKQPVDPAAWTPATWKARLLELVAGDGMPAAAIIEASDEIVGPWNTWDLPRVPVWRDDRTVLIGDAAHAVSPSSGQGASMAIEDAVVLGRCLEARSDVGAALAEYEGLRRGRVEKVVAYGKRSGSTKAAGPVGAVLRDAMTPLVMRLLYRKGNPQAWILDHRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9K7</accession>
    <name>U5W9K7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_31655</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560924.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31655"/>
    <dbReference type="EnsemblBacteria" id="AGZ44591">
        <property type="protein sequence ID" value="AGZ44591"/>
        <property type="gene ID" value="AFR_31655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6407"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_11"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="223"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="280"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="538" mass="55994" checksum="BC8F4BDC1EB629A3" modified="2014-01-22" version="1">MTAAAPQTTRTPRINPARRWIADRSLNTKIMIIVGSLALVTAMVGAVAVARMATLNNSVQDLYDGSFVSSQQLTTITTDVGTMHALVLTYGQTPDPAVLAQIKALDGQIKADNAAYRATTVNPALMDQTIFLWDKYDTARDAYLGAATSGNPATVLAARDTQLSPAIVRAKYNLQQLAIQETESAQENLAAAGSTYRSARNFLIIVLIAGLVLATALGLAIARGIVTRVRVVSRVIDRIADGDLTTRAELNSRDEIGRMGDQLDRATATLRTTISRITGSSHTLASSAQEMTEVSGRISVNSEQTSTRAGQVSLAADQVSANVATVAAASEEMSASIREIATSAADAAGVARGAVEVAQSANSTVAKLGVSSAEVGNIVKVITSIAEQTNLLALNATIEAARAGEAGKGFAVVASEVKDLAQETAKATEEISSRIQAIQTDTSAAVDAIAQIAEVIERINAYSDTIASAVEEQTATTSEIGRSVSEAAGGSTEIAQTITGVAEAAQSTNDGVGESRRTATELARLAEELQSLVAQFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9V6</accession>
    <name>U5W9V6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38350"/>
    <dbReference type="EnsemblBacteria" id="AGZ45924">
        <property type="protein sequence ID" value="AGZ45924"/>
        <property type="gene ID" value="AFR_38350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7758"/>
    <dbReference type="eggNOG" id="ENOG5032DZS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2285368_0_0_11"/>
    <dbReference type="OrthoDB" id="2203763at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="101" mass="10504" checksum="2D3D15E6B92A8909" modified="2014-01-22" version="1">MEEVAPVKLNYTIERPSTLLAAVCAPSVSWLPESAPVALLAVERAPVAQAHQVQIQAELVRTLVDVDGSNGTTGFVGSNDISAKKRMTVGGSGAPPRGRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAE3</accession>
    <name>U5WAE3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39520"/>
    <dbReference type="EnsemblBacteria" id="AGZ46158">
        <property type="protein sequence ID" value="AGZ46158"/>
        <property type="gene ID" value="AFR_39520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7995"/>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_354448_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="749" mass="77587" checksum="3D8F7385C7378D35" modified="2014-01-22" version="1">MALVRVYCGLASADETVRSATAGSQLTIAVVDDAGRLLGVHEISDDPAGYAQLGTLLVERTSGFADAAVAADSDDHMVTSLLTAAGRPLVVADDDSTDDFAERFADDESPDEISAPPAARRAVGLARALQAGVIAAATIPAPRELISYKPVLAAHVAMLNGRHSAASALREVLRELYPAALRAYPDPAHPLALAVLDALPEPGRLTGRGHDAEQVAEDVAAELTRAGAGTDDQVVAAVTALVVAISESPRRGGVNKSLSPAAADTVRHAIGAVRSCDSACEALVGTLVARISTQSATGAAGRRQAGSRRAAEPAASAPAARPSDTGTRLSRPSDTGTRLRPSDTGTRLGRRGRPEPAAASNAPAMPRPLTSPPVAPEPIMPPPMAPRPVTPPPVAPAAGLPSRTPNPGPASRPVSVPPPPPGMTPIVPGSRPSSVPPADSGQPFRPTLTNAAMSNARAERQRTVIPPRPSTRSTPQSPAANGATDYSLPMPAQRSTQDMPEPGSRANWPLMNTNDDAATPPAAAVSSPPTDGRVRPPWQDMPKEPPALRLVESQLSGLANTPADVTAISEPPPLRLVDDRPGRNGRAAMARRTTLTALDRSTTPPVPAEGDGDLLIFAAARSAWFTGHDEEASDNLEWANASDTGWRAAEKAATPSVAAETSAGLPKRVPQANLVPGSPLREERPLRIVRDAASIAAHTTGYFRGWRRGQEIGGYAIGGRPGRESAGGWDFSRDGGTPEDFRNAGYRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAH9</accession>
    <name>U5WAH9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alkaline phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_33465</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33465"/>
    <dbReference type="EnsemblBacteria" id="AGZ44951">
        <property type="protein sequence ID" value="AGZ44951"/>
        <property type="gene ID" value="AFR_33465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6773"/>
    <dbReference type="eggNOG" id="COG1785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008539_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="PTHR11596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666240" evidence="7">
        <location>
            <begin position="28"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="440" mass="45755" checksum="8BA49D4A2FA7A1AA" modified="2014-01-22" version="1">MVLAAAAIVAVPAVALAGDSHPDRTQAAKHAIQGGKARNVILLIGDGMGDSEITITRNYQVGAAGRLAMDKLPLTGAYTTYAVQKENPSLPEYVTDSAASGTGWATGHKTYNGAISVTPDGKPKPTILELAKQAGFRTGDVTTAELQDATPAVLGSHVVNRDCKGPDAMATCAVNDKVNGGAGSIAEQLVQTKPDVLLGGGKQFFDQTVKAGRYQGLTVAQQAQVNGYQIVTDATGLAAAKPGKPILGAFAKNNMDLEWVGPAPTRTGTAPAKCAVNTARTATQPHLVDMTGKAIDVLDKQTRNSRKGFFLQIEGASIDKQDHAANPCGQIGETVAFDAAIKKVLDYQKSHPDTLVVVTADHGHTSQIVEAPTMGYTATLTTHDGANMTISYATSEITGSQQHTGTEVRIAAGGPQAANVLGVTNQTDLFFTMKRALGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAP2</accession>
    <name>U5WAP2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40145"/>
    <dbReference type="EnsemblBacteria" id="AGZ46283">
        <property type="protein sequence ID" value="AGZ46283"/>
        <property type="gene ID" value="AFR_40145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8124"/>
    <dbReference type="eggNOG" id="COG3903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004665_5_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="780" mass="82352" checksum="2244F0CA734602F3" modified="2014-01-22" version="1">MHTGWATPGNNEYTSAEVHRAARVASAAHGGQILCSEATALAVTTWSTDLVFAGAQGGRAGGRAVGATDVDFLDLGPFRLRGFDDAERLFQLVAPGLGRDFPRPRTPCAPAHNLPAPVTGFIGRQAELAELRDLVGKHRLVTVAGAGGGGKTRLALAAAEELLTAYPDGVWVVDLAAELPEAALAAAMGVRAEPARPLLDSIVERCGSGRTLIVLETCEARQPAARELVRRLLAGCRRLDVLATSRVPLGVPGELVWRIPSLAPADAFALLVARAEAARGGRPSGSDEAADLARIASKLDGVPLAIELAADRLRLLSPAQLAGRLDDPIAALDAGRLDSHRHGSLTGNLDWSYHGLSDPAAELLRRLAVFAGPVDLSTVEWSDVDAFGALSELADRSLVEVVAGSRYRMSGQVRAYAARRLTAAGEEPAARDRHVDWALHTLAGVAVDTDGQPRTVSLTELAPYVGEWQAALRWSATGGSVRAGLRLAGALDPWWREHGGTRDGRDLLFRLYGRAEDADIAPAELASAYLVHAGLADDRDERTRFLDRAESVAQDIGHPALLVRAQAGRRVSLVEAGRLPEAEQVCRDAITLAEQSGVPGAALPSVVTLAELLWRRDALDEAAELLGSARQLEAGRPEDRGTRTVDWLLGMVALRRRDLVAAHDHLVVALRSRLRHGFRGASADAVAAIAVRCAIGGDPVTAAVLFGGVESARGARRAGQFGGFWFAQQASLRRALGDAAFDAAYASGTEIGFGRAVATALAVEHPDLEHGSVRFAQTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAS9</accession>
    <name>U5WAS9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative M23-family peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40370"/>
    <dbReference type="EnsemblBacteria" id="AGZ46328">
        <property type="protein sequence ID" value="AGZ46328"/>
        <property type="gene ID" value="AFR_40370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8171"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1101050_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003646">
        <property type="entry name" value="SH3-like_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51781">
        <property type="entry name" value="SH3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666246" evidence="1">
        <location>
            <begin position="30"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="SH3b" evidence="2">
        <location>
            <begin position="36"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="27902" checksum="B3AADEC92BD34670" modified="2014-01-22" version="1">MKKRRHQRTALALLATGGTLLLDPAAASAAPSVKVAGTGTVVTGGTALNARSGPSASSTRTGTVRNGTTVSIVCRVAGQFISGTVTNTGYWDRLSDNSFVSDAYVRRGDFAIPKCTTAAPLPAVAGAWMLPVTAGLISGFRTGPRPAHDGVDLAATRNTPIRAASSGTVIRVVCNVSKGSCDVDGNGSLSGCGWYVEVLHAGGIVTRYCHLVRRPEVSVGETVARGAVLGHVGTSGSSSGPHLHFEVHNGSPATRANAVSPITFMRARGLVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAU7</accession>
    <name>U5WAU7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_34185</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561432.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34185"/>
    <dbReference type="EnsemblBacteria" id="AGZ45095">
        <property type="protein sequence ID" value="AGZ45095"/>
        <property type="gene ID" value="AFR_34185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6917"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_4_1_11"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="9">
        <location>
            <begin position="4"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25880" checksum="CA67A8D2BE95E54D" modified="2014-01-22" version="1">MSVALVKVEMKLSVRDKVGPLWGVGFPLILLIILGSIPAMRKPEEANGGLTTFELYVPVLILFNIAMLALISLPNTLAGYREYGVLRRMRTTPVGPARVLAAQLLSNLAIAVVAAFLFLAVAGVAFGVDLPDNLAGFVAAWLVVAAALLSIGMLITAVAPGRGAATAIGTVLYFPMMFFAGLWVPITQMPPLLRDIAHLTPLGAGMQAFQDAYAGSFPSAQPLLVLLGYALVCSTIAVRTFRWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB37</accession>
    <name>U5WB37_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative flagellar biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">flhB</name>
        <name evidence="3" type="ORF">AFR_40525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562691.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40525"/>
    <dbReference type="EnsemblBacteria" id="AGZ46359">
        <property type="protein sequence ID" value="AGZ46359"/>
        <property type="gene ID" value="AFR_40525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8202"/>
    <dbReference type="eggNOG" id="COG1377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041013_1_2_11"/>
    <dbReference type="OrthoDB" id="1424100at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006135">
        <property type="entry name" value="T3SS_substrate_exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029025">
        <property type="entry name" value="T3SS_substrate_exporter_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30531">
        <property type="entry name" value="PTHR30531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01312">
        <property type="entry name" value="Bac_export_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00950">
        <property type="entry name" value="TYPE3IMSPROT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160544">
        <property type="entry name" value="SSF160544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="363" mass="38852" checksum="E7A8B21AFED59502" modified="2014-01-22" version="1">MSGEKTEQPTAQKLKKAKKEGQIGRSQDIGAWVGMLAASIMLPRTLGAAMDHSRELMEKVPDIIADPDPQVALGILKDGMMSAGWAVLPLALTMMAVGIAAAGAQGGIRVATKLFIPKFSRLNPLPGIKKMLGPQALWEGSKALIKTGVLAGVLYMTMKDIVPLLMTAGRLQVVSLLGVINDAVLSLIRAASVAGIVMAAADYFVVRRRTNKQLRMSKEEVKQEHKNTEGDPHVKGQIRARQMAMARNRQMADVPTADVVLVNPTHVAVALRYDPEKGAPRVIAKGQGAIAQKIRELATENRIPMVQDKALARALEKSVEVGMEIPAEFYGTVAKVLAFVMSLKTKGSAAGVHRNPNPTPAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBT2</accession>
    <name>U5WBT2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cell division protein ftsk/spoiiie</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_41575</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562901.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41575"/>
    <dbReference type="EnsemblBacteria" id="AGZ46569">
        <property type="protein sequence ID" value="AGZ46569"/>
        <property type="gene ID" value="AFR_41575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8412"/>
    <dbReference type="eggNOG" id="COG1674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003134_1_0_11"/>
    <dbReference type="OrthoDB" id="7548at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023836">
        <property type="entry name" value="EccCa-like_Actinobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023837">
        <property type="entry name" value="EccCb-like_Actinobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002543">
        <property type="entry name" value="FtsK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01580">
        <property type="entry name" value="FtsK_SpoIIIE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03924">
        <property type="entry name" value="T7SS_EccC_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03925">
        <property type="entry name" value="T7SS_EccC_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50901">
        <property type="entry name" value="FTSK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="FtsK" evidence="5">
        <location>
            <begin position="461"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="FtsK" evidence="5">
        <location>
            <begin position="818"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="domain" description="FtsK" evidence="5">
        <location>
            <begin position="1108"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="484"/>
            <end position="491"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="837"/>
            <end position="844"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="1125"/>
            <end position="1132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1326" mass="143227" checksum="504542342582A01B" modified="2014-01-22" version="1">MSTVVIKRAARRPAPEIPAGELAVDPPPEIPQAVGGRWTQAMMVLPMLGGSVAMAMMMGRGGGGAFSYVVGGLFGVSSLAMLATSFGGGGAPKKAEMMAARREYLRHLSALRRRVRDTASKQKIGLFYRHPDPGQLWSTADSHRVWERRPADPDFGVVRLAVGPQTLATPLIPPVTRPLEDLEPMTAGALRRFLDAYSVVPDLPVAVSLRGFARVFMRGQGPAGEGDARALTRAVLAQLAVFHAPEDLIIAVCAGPERRAMWEWVKWLPHNLHPSRTDRLGQVRLVASAGPDLEKLLEDVIGNRPRFNPAGVSTNGPHVVIVLDGADLKGATQLDDGIDGVTVLDLDDVPPRLLDRSLLVLEVKPGNAGRRELHTYAMDHAADVGMPDRLSEEEAEAVARRLAPLRLAAMSKNSDTPMATEMGLPELLNLGDPDTFSVAQAWLPRPNRDKLRVPIGLGADGGAIELDLKESAQDGMGPHGLLIGATGSGKSELLRTMVLALAATHSSETLNFVLVDFKGGATFSSLDRLPHTAAVITNLADELPLVDRMVDAIDGELIRRQELLRKAGNYGSLRDYEKARAGGAALPPLPSLLVICDEFSELLSAKPDFIDLFVQIGRLGRSLGVHLLLASQRLEEGRLRGLDTHLSYRIGLRTFSALESRAVLGVPDAYELPRSPGHGYLKFGTEPLIRFKAAYVSGAFRKAGSRGLGTARDGSAPQVLSYSTHYLPAPKPVKPAAPKPEELAEGESLLDVMVDRLNGQGPPAHQVWLPPLNQSAALDELLGPVTVDPVRGLAFANPELHGALQVPIALIDKPREQLRDVMWLQLGGSAGHVAVVGGAQSGKSTALRSLICGLALTHTPAEVQVYCLDFGGGSLGTLKDLPHVGGVYGRLDSVGVRRTVGEVSTLLNDRERRFGELGVDSMSSYRRRRAAQAGLNTGADADPFGDVFLVVDGWSTLRKDYDELESVITDIATRGLSYGIHVVTSAPRWMDYRPAIRDLFGSRVELRLGDPTDSIVNRRAAINVPEKAGRGLVEHPTDKNKSLHLLTVRPELSGAGETAALVKMIATNWSGAPAPRVRLLPPSLPYADIDLDRSTGLRLPIGIAESDLQPVEIDFSGDPHFLLFGDAECGKSSFLRALATSVTRRFAPEEARLILVDYRRSLMDLPESDHRIGYGIQAAKTLELMESVAGYMERRLPGPDVTAQQLRERSWWNGPELFVLVDDYDLVASGPTNPLTPILEYLPQARDVGLHLIVTRRAGGASRAMYDPVIQRLRELSSPGLVMSGPSDEGALIGPIRPTLMPPGRGRLMTRREGMRLIQLSHLPPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBX2</accession>
    <name>U5WBX2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Recombination protein RecR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">recR</name>
        <name evidence="9" type="ORF">AFR_41800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the RecR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562945.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41800"/>
    <dbReference type="EnsemblBacteria" id="AGZ46614">
        <property type="protein sequence ID" value="AGZ46614"/>
        <property type="gene ID" value="AFR_41800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8459"/>
    <dbReference type="eggNOG" id="COG0353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060739_1_0_11"/>
    <dbReference type="OrthoDB" id="1661836at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01025">
        <property type="entry name" value="TOPRIM_recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00017">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000093">
        <property type="entry name" value="DNA_Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023627">
        <property type="entry name" value="Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023628">
        <property type="entry name" value="Rcmb_RecR_C4-type_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015967">
        <property type="entry name" value="Rcmb_RecR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034137">
        <property type="entry name" value="TOPRIM_RecR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30446">
        <property type="entry name" value="PTHR30446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02132">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111304">
        <property type="entry name" value="SSF111304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00615">
        <property type="entry name" value="recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01300">
        <property type="entry name" value="RECR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="8">
        <location>
            <begin position="79"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="7">
        <location>
            <begin position="56"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="197" mass="21476" checksum="867558D7B7020C2E" modified="2014-01-22" version="1">MYEGAIQDLIDELGRLPGVGPKSAQRIAFHVLSADPADVTRLANSLRRVKELVRFCTTCFNVAESEQCRVCRDTRRSNEVICVVEEPKDVVAIERTGEFRGRYHVLGGAINPLEGVGPDNLRIRELLIRLGAGEVKELILATDPNTEGEATATYLAIMVKPMGIAVTRLASGLPVGGDLEYADEITLGRAFEGRRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC10</accession>
    <name>U5WC10_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Fifty-four homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ffh</name>
        <name evidence="10" type="ORF">AFR_36370</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="7">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561866.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36370"/>
    <dbReference type="EnsemblBacteria" id="AGZ45530">
        <property type="protein sequence ID" value="AGZ45530"/>
        <property type="gene ID" value="AFR_36370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7360"/>
    <dbReference type="eggNOG" id="COG0541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_11"/>
    <dbReference type="OrthoDB" id="656414at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004780">
        <property type="entry name" value="SRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF7">
        <property type="entry name" value="PTHR11564:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00959">
        <property type="entry name" value="ffh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54" evidence="9">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="433"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="445"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="192"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="250"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="517" mass="55381" checksum="A06CAD2DFD347226" modified="2014-01-22" version="1">MFDTLSDRLSGIFTKLRGKGKLTDADIDATAREIRLALLEADVALPVVKSFIANLKERARGAEVSQALNPAQQIIKIVHDELITVLGGEGRRLQFAKQPPTVIMLAGLQGSGKTTLAGKLSRWLKGQNHQVLLVAADLQRPNAVNQLQVLGGRAGVDVYAPAPGNGVGDPVQVAKDSIEHAKRTAKDIVIVDTAGRLGIDADMMQQARDIRDAVDPDEVIFVIDAMVGQDAVTTAEAFRDGVGITGVVLSKLDGDARGGAALSVRHVTGEPILFASTGEKLEDFDVFHPDRMASRILGMGDVLTLIEQAEQAFDEDQKEKMTSKLLGGEQFTLEDFLDQLIAVRRMGPIANVLAMMPGMGQMKGQLDELDDSHFDRVTAIIRSMTPGERTNPKIINGSRRARIANGSGVTVMDVNQLLNRFTDAQKMMKQMGGMMGLPGGNRRKATKSPKNKRKGTKGGNRPRVGGMPGGFPGGMPQLPPGMDPSALGGGPGGLPPGFKLPKLDFNKLTKKPDDDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC39</accession>
    <name>U5WC39_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_36420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561876.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36420"/>
    <dbReference type="EnsemblBacteria" id="AGZ45540">
        <property type="protein sequence ID" value="AGZ45540"/>
        <property type="gene ID" value="AFR_36420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7370"/>
    <dbReference type="eggNOG" id="COG0347">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002332">
        <property type="entry name" value="N-reg_PII_urydylation_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039144">
        <property type="entry name" value="GlnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00496">
        <property type="entry name" value="PII_GLNB_UMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR039144-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="112" mass="12167" checksum="5D00C121A13A4457" modified="2014-01-22" version="1">MKLVTAVIKPYQLDAVKEALHALGVAGLTVSEVQGYGRQKGHTEVYRGAEYTVEFLPKIKVEVLTDEIDVEKVVDAVTTSARTGKIGDGKVWVTAVEDVIRVRTGERGLDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC92</accession>
    <name>U5WC92_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561951.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36795"/>
    <dbReference type="EnsemblBacteria" id="AGZ45615">
        <property type="protein sequence ID" value="AGZ45615"/>
        <property type="gene ID" value="AFR_36795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7443"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_2_0_11"/>
    <dbReference type="OrthoDB" id="688156at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_3" evidence="1">
        <location>
            <begin position="43"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_lid_2" evidence="2">
        <location>
            <begin position="249"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="36325" checksum="FACE252089BF3492" modified="2014-01-22" version="1">MTDIAADTDTRMLEQTLFEVKKVIVGQDRLVERLMTALLAGGHCLLEGVPGVAKTLAAETLAVAVGGSFHRIQFTPDLVPSDILGTRIYRASKENFDVELGPVMANLVLADEINRAPAKVQSALLEVMAEGHVSIGGRTYQTPQPFLVLATQNPIESEGVYQLPEAQRDRFLMRVVVGYPTDQDELGILYRMGGVRPTARPVLDTGKLLALQARARDVFVHHALAEYVVRLVLATRDPARFGLPELAGQLAYGASPRATLGLVAAGRALALLRGRDYVLPGDLLDIAPDVLAHRLVLSFDAVADGIEAESIIARIVESVPLPVIAPAQEQQGPGLGIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCJ0</accession>
    <name>U5WCJ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Beta-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_36910</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36910"/>
    <dbReference type="EnsemblBacteria" id="AGZ45636">
        <property type="protein sequence ID" value="AGZ45636"/>
        <property type="gene ID" value="AFR_36910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7468"/>
    <dbReference type="eggNOG" id="COG3250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005015_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Glyco_hydro_2_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="SSF49303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_2" evidence="4">
        <location>
            <begin position="196"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="801" mass="89227" checksum="31F26E38B7C030CE" modified="2014-01-22" version="1">MTSYRPLHDGWALTGGDVDSVAAVVPGCVHTDLLAAGLIDDPYLDENEKKLAWIGRTDWVYETVFQHTRGDGRADLVCAGLDTVATVILNGVELGRTANMHRGYRFDAREVLRDGENQLRVRFDSAYSYAEAQQERLGDRPNAYPEPFNFIRKMACNFGWDWGPTLVTAGIWQPIGLHSWSVARLAEVRPQVAVADGVGTVDVRVRLERETAGPVTLAATVAGRRMQIQTEEDEAVLSLTVDDPQLWWPRGYGNQPRYDLDVTLAGPDAAPLDTWHRKIGFRSVRVDTTPDSEGSAFTLLVNETPVFVRGVNWIPDDVFANRVTRERLAARFTQAIDANVNYLRIWGGGRYESEDFYDLADELGLMVGQDFLFACAAYPEEEPFATELAAEARENVVRLSSHPSLMMWTGNNENIWGHEDWGWKDSLGDKSWGAGFYFDVLPRIVAEEDPSRPYWPGSPYSGRNDKHPNDPAYGTTHIWDVWNTDDYLKYREYRPRFVAEFGYQAPPVYATLRRAISDEPLTHDSPGMQAHQKAIGGDGKLQRGLDAHLPAPRDFDDWHFLTQLNQARALSVGIEHFRSLRPVCMGAIMWQLNDCWPVTSWAAVDGDGRKKPLWYALRRSYAPRLLTVQPRDGVPHLVAVNDSAEDWPLSATVSRRTVAGVVEAEVAIGATVGPGAAITLPLPAEVGAAGDAAHEFLVAEAEEHRAWWFFAEDKDIAWPPAKFDATFEAIGDDARVRITAHNILRDLTLHPDRLDPSTEVDESGVTLLPGEQATFMIRGGAGLEPTALTTRPVLRCVNDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDK8</accession>
    <name>U5WDK8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46080">
        <property type="protein sequence ID" value="AGZ46080"/>
        <property type="gene ID" value="AFR_39130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7919"/>
    <dbReference type="HOGENOM" id="CLU_2598126_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="79" mass="7933" checksum="165CB696E4E16C58" modified="2014-01-22" version="1">MLTLTLGTALSKSATEDGSGALSALIICMILVMVVIAKLFGGMIAPFKEVIKAAVAALGALLLTGVLVVMLVTALVMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF29</accession>
    <name>U5WF29_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46536">
        <property type="protein sequence ID" value="AGZ46536"/>
        <property type="gene ID" value="AFR_41410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8380"/>
    <dbReference type="HOGENOM" id="CLU_932660_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="29929" checksum="B2024F8A78377941" modified="2014-01-22" version="1">MGRGGSTEYGGGTYGGAEYGGGYDPGSAWADQSGAAPQYAAAQPADYTRGEDYLSDEHTGGDNYALSGQHTGSGNYGATGRDNYAAGGEHTGGDNFAAGGERTGGDNFAAGGERTGSSDYATSGSYSTGGNYGTGGSYATDGNYAAGSSYAGGADYATSSDYAISQDYTGAGNANGAGVGTSYGSGTGYDSAPVVEYQAGSNDAAPQRASRRHAAPDTGTDIGRYGLADQAQDGGYEATGYGYGVPLTEDRTGAYHSGGTSGYIDNGSYVTFGPQSEQGRPETGGYYGTDQRDEHGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNC2</accession>
    <name>U5VNC2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phytoene synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carotenoid biosynthesis; phytoene biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357411.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00895"/>
    <dbReference type="EnsemblBacteria" id="AGZ38468">
        <property type="protein sequence ID" value="AGZ38468"/>
        <property type="gene ID" value="AFR_00895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.178"/>
    <dbReference type="eggNOG" id="COG1562">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_1_2_11"/>
    <dbReference type="OrthoDB" id="1449625at2"/>
    <dbReference type="UniPathway" id="UPA00799"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:farnesyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044843">
        <property type="entry name" value="Trans_IPPS_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01212">
        <property type="entry name" value="Phytoene_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="33332" checksum="8F70428471834363" modified="2014-01-22" version="1">MEIDLAAAYERCREIHKRHGRTYYLATRLLPSWKRRHVHALYGFTRYADEIVDRTEDLPPAERAARLNAWSDEFLAGLAGAPTDDPLLPAVLHTIAVFDLDRSDFASFLRSMAMDLTVTSYATYDDLLDYMEGSAAVIGTMMLPILGSSDPAAAREPARQLGFAFQLTNFIRDVAEDLDRGRTYLPDSHLAKFGVTRDDLQEARALGRSTPQIKALIKYEVARADEHYAAAAPGIPLLLPGSQACMRTAFELYGGILDEVVAADYDVFVRRATVPNQRRARVAVRSLLTRAGTPVAASVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNQ8</accession>
    <name>U5VNQ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sugar ABC transporter periplasmic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357506.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01370"/>
    <dbReference type="EnsemblBacteria" id="AGZ38563">
        <property type="protein sequence ID" value="AGZ38563"/>
        <property type="gene ID" value="AFR_01370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.274"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_8_0_11"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665160" evidence="1">
        <location>
            <begin position="32"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="451" mass="48949" checksum="9D2C1513CA91312E" modified="2014-01-22" version="1">MRRRLLATTFALGALLLSSACSGSGDSTEEAATGPVTITVNGLPPDTDPVNRKNFEEDVTKFMDKYPDIKIEAKEGMMDPQTFSAKLAGGQLEDVFYVYFTDPQALIRKRQVSDISKYLGEFPNTKDLKPELLRIFSDESGHAYGLPSQNYSMGLVYNRELFKKAGLDPATPPRTWAEVREAAKKINALGGGVVGYGDYSKNNTGGWHFTAETYSLGGDVATQDGETWKAAFNTATGKQVLQQLKDMRWTDNSMGQKQLLEYSDLLQMMGSNKLGMYLGTGSDIVNIVNQYKGKFDTYGLGSIPDGKGTLGGGDGYMFKAGLSPAKIKAGLTWLSYKTVAPDRIAESLGRANKNKEPVGLPQPNIWVAGSPSDKTLVAERDKLKNVPVENFAPFAATETSIPLKLEPPLAQQVYAVLDVPMQKVLTDRNADIDKLLADAEAQVNGVLGAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNS3</accession>
    <name>U5VNS3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH_17 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014687221.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00775"/>
    <dbReference type="EnsemblBacteria" id="AGZ38444">
        <property type="protein sequence ID" value="AGZ38444"/>
        <property type="gene ID" value="AFR_00775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.156"/>
    <dbReference type="eggNOG" id="COG2452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196700_2_1_11"/>
    <dbReference type="OrthoDB" id="2076478at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="11"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="69" mass="7741" checksum="C2E70CA5ADD960B5" modified="2014-01-22" version="1">MASRTHDPEPLLTPAEVASMFRVDPKTVTRWAKAGKLSAIRTLGGHRRYRESEVRALLQGQIPTQRQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP58</accession>
    <name>U5VP58_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-octaprenyl-4-hydroxybenzoate carboxy-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357512.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01400"/>
    <dbReference type="EnsemblBacteria" id="AGZ38569">
        <property type="protein sequence ID" value="AGZ38569"/>
        <property type="gene ID" value="AFR_01400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.282"/>
    <dbReference type="eggNOG" id="COG0043">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023348_4_1_11"/>
    <dbReference type="OrthoDB" id="1012819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022390">
        <property type="entry name" value="HBDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="PTHR30108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03701">
        <property type="entry name" value="mena_SCO4490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00148">
        <property type="entry name" value="TIGR00148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="487" mass="53922" checksum="926497315338518C" modified="2014-01-22" version="1">MASKGFPYSDLQSFIAALEKAGELRRVSVPVDPTLEISEVVTRTVRAGGPALLFERPTRGEMPIVINLFGTEKRMAMALGVDSLDEIGDRIGSLIQPELPVGWSGIREGIGKVLQLKSIPPRKVKSAPCQEVVLKGDEVDLGKLPGLQVWPGDGGIFHNYGLTHTKHPETGKRNLGLYRLQQHSKNTLGMHWQIHKDSTAHHAVAERRGERLPVAIAIGCDPVVSYAASAPLPGDIDEYLFAGFLRGERVEMVDCLTVPLQVPAEAQIILEGYLEPGERAPEGPFGDHTGFYTPVEPFPVLHIECMTMRKKPVYHSIVTSKPPQEDHGLGKATERIFLPLLKMLIPDIVDYDMPAAGVFHNCVIVSIRKRYPKHAQKIMNAIWGAHLMSLSKLIVIVDEDCDVHDYNEVAFRAFGNVDYDRDLLVTQGPVDHLDHASYQQFWGGKAGLDATRKLPAEGYTRGWPEEMTMAPEVAALVDKRWKEYGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP95</accession>
    <name>U5VP95_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357552.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01600"/>
    <dbReference type="EnsemblBacteria" id="AGZ38609">
        <property type="protein sequence ID" value="AGZ38609"/>
        <property type="gene ID" value="AFR_01600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.322"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_11"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="16"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="315" mass="34004" checksum="2277D0C48F1FB496" modified="2014-01-22" version="1">MSYRRLGTSGLVVSVVGIGCNNFGRKLDVDGTRAVVDAAFDAGITLFDTADIYGSPHGASEEFLGAALQGRRDEIVLATKFGMDMEGLNGADFGARGSRRYIVRAVEASLRRLGTDYLDLYQMHQPDESTPIEETLSALDDLVRSGKVRYLGNSNFAGWQIADADWTARTAGHSPFISAQNQYSLLHREVEDEVVPACEQFGLGLLPFFPLDSGLLSGKYHRGQTAPEGSRLSQDRYQRWLDGADWDAIEALTKFGEERGHSLLEVAIAGLLAQPAVASVIAGATSAEQVRANAEAGTWRFTAEDVAALDQILAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ79</accession>
    <name>U5VQ79_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Na(+)/H(+) antiporter NhaA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Sodium/proton antiporter NhaA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nhaA</name>
        <name evidence="9" type="ORF">AFR_03415</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29251"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357915.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03415"/>
    <dbReference type="EnsemblBacteria" id="AGZ38972">
        <property type="protein sequence ID" value="AGZ38972"/>
        <property type="gene ID" value="AFR_03415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.687"/>
    <dbReference type="eggNOG" id="COG3004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015803_0_0_11"/>
    <dbReference type="OrthoDB" id="1805428at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015081">
        <property type="term" value="F:sodium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01844">
        <property type="entry name" value="NhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023171">
        <property type="entry name" value="Na/H_antiporter_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004670">
        <property type="entry name" value="NhaA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30341">
        <property type="entry name" value="PTHR30341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06965">
        <property type="entry name" value="Na_H_antiport_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00773">
        <property type="entry name" value="NhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0050">Antiport</keyword>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ38972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="408" mass="43094" checksum="C3AA51649C11B31B" modified="2014-01-22" version="1">MSLFGRGTWPEARRIAELLRAETVGGVLLLVAALAGLAWANSPWGDSYRSLSALHVGPLTLAEWAADGLLAIFFFVAGLELKREFVAGDLRDPRRAALPVVAAVGGMIAPAVIFTVINLNSGALAGWATPTATDIAFALAVLAVIGTHLPAALRTFLLTLAVVDDLLAITIIAIFYTSSLHLLPLLLALLPLAGFGLLVQRGVHRWWLLLPPAAVTWFLVHESGIHATVAGVLLAFTVPVHRDGMAEHFERRIRPLSSGFAVPVFAFFAAGVSVAAAGGLGASLRDSVTIGMIVGLVAGKTIGITGATWLVQRFTRASLDPGLSWWDVLGLSLLGGIGFTVSLLIGELAYGSGTVRDGHAKIGILLGSLISALLATIVLRLRDRHYRLIAVEESHDADEDGIPDVYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR24</accession>
    <name>U5VR24_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_05050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358802.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05050"/>
    <dbReference type="EnsemblBacteria" id="AGZ39299">
        <property type="protein sequence ID" value="AGZ39299"/>
        <property type="gene ID" value="AFR_05050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1020"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_11"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="9"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="53"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="37217" checksum="D9841B6E90B204F7" modified="2014-01-22" version="1">MSDRDDVVLETKGLVKHFPITQGIVFKSKVGAVRAVDGVDLQLRRGETLGVVGESGCGKSTLAKLLVGLEKPTEGAINVRGEDMVKLRGGNLRRARRNIQMVLQDPYTSLNPRMTVGDIIGEPFEIHPEVTPRKGRASAVQDLLDTVGLNPDHVNRYPHQFSGGQRQRIGIARALALKPEIIVCDEPVSALDVSIQAQVINLLEGLQKDFGLSYIFIAHDLSVVRHISNRVAVMYLGKIVEIGNDQQIYEQPTHPYTQALLSAVPVPDPRLRGQRDQIVLEGDVPSPANPPSGCRFRTRCWKAQSVCAEQEPLLQIRDRSPHPSACHFAEPRDVVHAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR88</accession>
    <name>U5VR88_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretion system protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359019.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05995"/>
    <dbReference type="EnsemblBacteria" id="AGZ39488">
        <property type="protein sequence ID" value="AGZ39488"/>
        <property type="gene ID" value="AFR_05995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1218"/>
    <dbReference type="eggNOG" id="COG2804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013446_10_1_11"/>
    <dbReference type="OrthoDB" id="749544at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037257">
        <property type="entry name" value="T2SS_E_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007831">
        <property type="entry name" value="T2SS_GspE_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05157">
        <property type="entry name" value="T2SSE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160246">
        <property type="entry name" value="SSF160246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00662">
        <property type="entry name" value="T2SP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="554" mass="60307" checksum="C80B320F50D4B00E" modified="2014-01-22" version="1">MDQTFRPLLDALKQIGIDPVAVDAAADEAQRSGRSVRAVLINDQVVTEEQLTSAAATAFGINTLDLVGFSPDPAALKRIPLPVVLRHRVLGLSMSDGELVVGVTDPGDVVALDDIRASTGMTIRPVVVARSEVRRIIERLQREDSDLGDLADQNLDDLPMAAQATSSDDAPIVRYVNNLIEQAVQNRASDLHLEPTEDEMRVRYRIDGVLHELDTVPRGVMAALTSRLKIMSGVDITEKRIPQNGRITVLIRDRQVDLRTATLPTVWGEKIVLRVLDTGGIDLDLAKLGFTQHNLTRFAESFTKPHGMLLVTGPTGSGKSTTLYATLGRISKPEINVITVEDPVEYRLRGVNQVQVNAKAGLTFAAVLPAILRSDPDVVLIGEIRDGNTAQIAVEASLTGHLVLSTLHTNDAPGAVTRLTEMGIEPFLVGSSLDCVLAQRLARRLCDWCKEAYLPTEEELEGARWPLHDLKIPEALYRPVGCRNCANTGYRGRIALHEVMPISPEIESLTIRRASSNEIREVALAQGMYELRSDGLAKAAGGLTSIREVSRVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VST1</accession>
    <name>U5VST1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359455.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08175"/>
    <dbReference type="EnsemblBacteria" id="AGZ39924">
        <property type="protein sequence ID" value="AGZ39924"/>
        <property type="gene ID" value="AFR_08175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1668"/>
    <dbReference type="eggNOG" id="ENOG503398G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128798_0_0_11"/>
    <dbReference type="OrthoDB" id="1817677at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="176" mass="19230" checksum="4EA97CD4EE93C209" modified="2014-01-22" version="1">MSLFRRRPKLPAQLSPALEAEERVLAWAYVGEPGEHVLVATNRGLWLPDGKRLGWHEIHKVAWSGRELLITPAEVAQEREGYTVQIDGPVLGALLLEPGELPDQVRTRVTRSVAYTSHHPLPPNGGVRVVGRRVSGVDGLSWAVRYDAKTAVDAEPVVEVTDQLVASAREATTPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT61</accession>
    <name>U5VT61_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357784.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02760"/>
    <dbReference type="EnsemblBacteria" id="AGZ38841">
        <property type="protein sequence ID" value="AGZ38841"/>
        <property type="gene ID" value="AFR_02760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.554"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035614_3_0_11"/>
    <dbReference type="OrthoDB" id="1439085at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="366" mass="39901" checksum="FEB758B95AFC698E" modified="2014-01-22" version="1">MDDWSELQGKVQRTIDGLVGAGREIGVQVAAYLDGALIVNVVSGLADESTGQMVTPDTPFFSFSTGAGLTSTVVHVLAEKGQLDYDLRIADVWPEYARHDKKKTTLRHALSHSAGVPQLPSFTSPEDFLDWDRMCRTIAGSAPMWEPGTRHGLHTWTYGWLIGEVVRRAVHRPIAWVLAEDIARPLDADRELFFGVPTEQVNRVARLKDRNWNAALELLSERIENFDKICPLGVRPNAMLANRRDILRTDIPSAATVSARGIARMYAALMGEVDGVRLISPERLKEITTVVTDGPDWAFGGDLPKSLGYAYQLGGARYGWSGNGGSLAGFYPELNLSIAVTKNYLGSGDDDAMEGVAALIYAAVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTW9</accession>
    <name>U5VTW9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_10150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10150"/>
    <dbReference type="EnsemblBacteria" id="AGZ40319">
        <property type="protein sequence ID" value="AGZ40319"/>
        <property type="gene ID" value="AFR_10150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2067"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="146"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="414" mass="41931" checksum="2CD89AD91BAC96E4" modified="2014-01-22" version="1">MKPRDIALAVAVAAAWGINFVVIEIGLDHFPPLLFSALRFTVAAFPAVLFIGRPPARWRWVIAVALALGVVKFSLLFAGMAAGMPAGLSSLVLQSQAVFTTIFAVLLLRERPGRLRLAGLAIAALGIGVVATRLGTDGLPAGAFSLVLGAAVAWGVSNIATRRAAPADTLRFMVWVSAVASLPLIALSLLIEGPAADLAALRAINPAAVAALLYVALISTLAGFGAWGSLIKRYGASAVAPFSMLVPVFGIASAALFLNEQIHATDLAGAVLVVGGVLLGVLRKASALTPPRAVSDNEDGRVPPSLPLPIAGPSSALLQPVVSDDRGRESGPSLPLPVAAPSSALPQPVVSVDGGLGSGSSVALPSSTSATTSQRPILVEVGEPGLVAGSTRPVVAPTGALPGFSPAPQERHQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUE4</accession>
    <name>U5VUE4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40442">
        <property type="protein sequence ID" value="AGZ40442"/>
        <property type="gene ID" value="AFR_10765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2194"/>
    <dbReference type="HOGENOM" id="CLU_1736636_0_0_11"/>
    <dbReference type="OrthoDB" id="2167871at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045629">
        <property type="entry name" value="DUF6232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19744">
        <property type="entry name" value="DUF6232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="17131" checksum="A8DAAE020ECD86F7" modified="2014-01-22" version="1">MYQGPELGGGGGGRRPRWRIIYLDDTIIITSWYVEIDGARIPLADLHDVLVCLTYRYPLVKVTAVMGLIEAAIAGTSAAVLQSGWMLLAGVFSACTVAMGAITDFRKNPRFMTIEATWRGKRIVLFRTRDKQKFGQVRRALIRAIEDNRGFRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUM8</accession>
    <name>U5VUM8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc transporter 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_05730</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358966.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05730"/>
    <dbReference type="EnsemblBacteria" id="AGZ39435">
        <property type="protein sequence ID" value="AGZ39435"/>
        <property type="gene ID" value="AFR_05730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1162"/>
    <dbReference type="eggNOG" id="COG0053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021126_0_1_11"/>
    <dbReference type="OrthoDB" id="381612at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040177">
        <property type="entry name" value="SLC30A9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414">
        <property type="entry name" value="PTHR13414"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="33741" checksum="67D105E24546B58F" modified="2014-01-22" version="1">MSAGGGTRAIIAALAANLGIAATKLLAWLLTGSSSMLAEGIHSIADSGNQALLLLGGKRATRKATPEHPFGYGRERYIYAFIVSIVLFSLGGLFALYEGYHKVQHREGIESWQWVPVTVLVIAIGLESFSFRTAIKESNHTRGKTTWVNFVRRAKAPELPVVLLEDAGALVGLVLAFFGVVLTLITGNGVWDGIGTLAIGVLLVVIAIILAIETKSLLLGEGANPEDVTKIEQAIVAGPGVERIIHMKTLHLGPEELLVAAKIAVPATESAAEVARHINETEARIREALPIARVIYLEPDIYSPQAPKPAASEAPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUQ2</accession>
    <name>U5VUQ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360772.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12135"/>
    <dbReference type="EnsemblBacteria" id="AGZ40713">
        <property type="protein sequence ID" value="AGZ40713"/>
        <property type="gene ID" value="AFR_12135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2468"/>
    <dbReference type="eggNOG" id="COG3087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_852094_0_0_11"/>
    <dbReference type="OrthoDB" id="1900996at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="31703" checksum="0F248394C39828BA" modified="2014-01-22" version="1">MGAWSAGLINAMIDGVVTWMTASTMAALNWVLSLLSNTVFTSPDVTGLPQVSFMSRRALLAPNACLALIVTVVGFLAMTHGTVQDRYSLKELLPRMLIGFAAANLSTPILSTAIAAANALTAALTGDPLSSPDSFAQIKRIITDVAGNPEQLLVALVLRELAVLLVLLVISWLGRLSVLIVAGAVAPIALLCHALPQTDPVARTWWRALGGCLLIQVLQAVALRTAVATLLSGEASLPALGLPHDPSGLFNLLMTCFLLWLVIRVPKWVSRSFGGNAGRATSMPICQGMAPQSGSPVWPVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUU3</accession>
    <name>U5VUU3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme F420-dependent NADP oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360527.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11315"/>
    <dbReference type="EnsemblBacteria" id="AGZ40552">
        <property type="protein sequence ID" value="AGZ40552"/>
        <property type="gene ID" value="AFR_11315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2306"/>
    <dbReference type="eggNOG" id="COG2085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124419_0_0_11"/>
    <dbReference type="OrthoDB" id="1768241at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="184" mass="18930" checksum="DADD418FAB3DD30E" modified="2014-01-22" version="1">MNITVIGRGNIGGGLADLWERAGHKVERLGRGGGDAGAADAVLIAVPGDAVQAALSSVTGLSGKTVLDATNLYGEVKPPAGFGSNAEYVKSVTGGPTAKVFNTNIAALYPQIADARARPSNLWSGDEEARRVVEQLNTDAGYEAVRLGDLSQAATQESIAHAIIAIAQNGLGAFVYRMATPEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV27</accession>
    <name>U5VV27_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360778.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12165"/>
    <dbReference type="EnsemblBacteria" id="AGZ40719">
        <property type="protein sequence ID" value="AGZ40719"/>
        <property type="gene ID" value="AFR_12165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2474"/>
    <dbReference type="HOGENOM" id="CLU_1412503_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="20082" checksum="2BA561A35535E7A4" modified="2014-01-22" version="1">MPTDSNDEPGTYAIPRAGLPWLVCVPAGTGTDLSPQLAQLILDVHTAVGDVVVDIDDDSAFAAAAAETGRRHHALGGATGLNAFGHAAGYIDLLLMHWPQPNADPRWLLLTCRTLRRNEGCLVVAVKAVNQRRVAALSALTGAAHTAGLNLVDHIAVIDLSADNFLTQPGTAAERTPRVDADLLVFVAATPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV45</accession>
    <name>U5VV45_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Luciferase-like, subgroup</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40739">
        <property type="protein sequence ID" value="AGZ40739"/>
        <property type="gene ID" value="AFR_12265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12265"/>
    <dbReference type="HOGENOM" id="CLU_2566145_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="81" mass="9043" checksum="FE02B6EB1DF1093F" modified="2014-01-22" version="1">MTAIGLVEQAETEVVPVLRKEFAALKPAPVPDAPTHESLKDERGTGRAAGRRAARPWTNLRPGQVTGLTRRSCRWRAKLRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV55</accession>
    <name>U5VV55_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360942.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12985"/>
    <dbReference type="EnsemblBacteria" id="AGZ40883">
        <property type="protein sequence ID" value="AGZ40883"/>
        <property type="gene ID" value="AFR_12985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2637"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="24751" checksum="85E4C56DC4BD6100" modified="2014-01-22" version="1">MPAALITGGTTGIGRATAELLHTRGYRVAVTGQNPDSLARARRELPGDVLVVRSDARDLADTDALVTMVSDRFGSLDLLFLNAGIFCPAPVGGVTEESFDEQADVNFKGQFFTLQKALPLMNDGGSIVLTVGIGARRGSPGATVGAATRGALLAMVPSLALELAPRRIRVNAVSPGATETPLLDKLGVPESGRDAMRAGIPFERFGTGQEIAEVVAFLASAAAGYVTGQDVVVAGGYGLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVV7</accession>
    <name>U5VVV7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Heme chaperone HemW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_07830</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359386.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07830"/>
    <dbReference type="EnsemblBacteria" id="AGZ39855">
        <property type="protein sequence ID" value="AGZ39855"/>
        <property type="gene ID" value="AFR_07830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1599"/>
    <dbReference type="eggNOG" id="COG0635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027579_1_0_11"/>
    <dbReference type="OrthoDB" id="1130951at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004559">
        <property type="entry name" value="HemW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="PTHR13932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00562">
        <property type="entry name" value="HemN-like__clustered_with_heat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00539">
        <property type="entry name" value="hemN_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="24"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ39855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="403" mass="42956" checksum="A7CB064DCC80D461" modified="2014-01-22" version="1">MPGPLPDGEPVPSDGSLPESALGAVGRRGFGVYVHVPFCASRCGYCDFNTYTASELGGGTSREGYADAVLAELKLAATVTGAPVDTVFVGGGTPTLLDPDDLGRILEGIDKTWGLTANAEVTTEANPESVTPRSLQALRKAGFTRISLGMQSAAPAVLAILDRKHTAGRAPQAAIEAREAGFDHVNLDLIYGTPGERPEDFDASLRTVIDAGVDHVSAYSLIVEDGTRMAARMRRGELPYPEDDVAADRYLAAEQALSGAGFSWYEVSNWATTPDARCRHNMLYWTGGDWWGLGPGAHSHVGGVRWWNVKHPSAYAKRVEEGVSPGLEREILTDEDRHVEDVMLRVRLRDGLPLATLDATGLTGAAKALADGLLDPAAYESGQVVLTLRGRLLADAVIRDLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVZ4</accession>
    <name>U5VVZ4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">POLIIIAc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359426.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08030"/>
    <dbReference type="EnsemblBacteria" id="AGZ39895">
        <property type="protein sequence ID" value="AGZ39895"/>
        <property type="gene ID" value="AFR_08030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1639"/>
    <dbReference type="eggNOG" id="COG1387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021963_0_0_11"/>
    <dbReference type="OrthoDB" id="1309717at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="1">
        <location>
            <begin position="92"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="571" mass="61270" checksum="0A87BF3ECF12CAF7" modified="2014-01-22" version="1">MGHGHHHDHDHSHDAAAGTDVPAALDLNVPDSELSPSDVSRRGFLRNAGLLGAGAAASVLATPSAAAAAGLPHSHGPVGGGTEKGGFRWLAGDHHIHTQHSSDGLYRVIDQAGHGRAFGLDWVVITDHGSNAHAKIGVDKVNPDILATRKQLPDLLTFQGLEWNIPAAEHATVFVHPGRNEVAVLKQFEQTFDGSQLPATNTEAQNEAAAIAGIKFLGEQVRQRKVDGALFLANHPSRRGIDSPHEIRNWRDADPTVAIGMEGAPGHQAAGIKAPNGRGGARGYYDNNPSAASFAGFPLETYRSWGGFDWMTSTVGGLWDSLLAEGRAWWVTVNSDSHVNYLDQSDRGPGSDFEANGRYNDPVYTGTVNTGAGDFWPGFYGRTSVGATSFSYKAVMDGLRAGRVWVDHGRLIKTLDARVRVAGDRRPSTGTPLGGVLHVRRGTATELTLEIDLQDMPNWAQFIPVLSRVDVIVGQVTGPVAARDTFTAPTAKVVKSYEVSPGAKRLTVTYSLGRLDKPFYLRLRGTDGKRTQPGLMGASVDPYGPQLDVIGDADPWGDLWFYTNPIWVLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWK9</accession>
    <name>U5VWK9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08980</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359618.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08980"/>
    <dbReference type="EnsemblBacteria" id="AGZ40085">
        <property type="protein sequence ID" value="AGZ40085"/>
        <property type="gene ID" value="AFR_08980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1827"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038683_1_1_11"/>
    <dbReference type="OrthoDB" id="626204at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="19"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="406" mass="41311" checksum="0007B6E3D1A8EC7F" modified="2014-01-22" version="1">MTVTAAPRHERATSTRHGTGFWLIAAVFAVSMAFSTVPTPLYPIYQREDGFTPFTVTIVFAVYAVGVVTSLLLAGHISDWVGRRRVLLPALALEAVAAVLFLVWPALPGLLIARFVTGLGVGMITATATAYLLELHSAHKPNAGRGRFELVSAAANLGGLGAGTLVAGALAQFVDAPLRTPYLVFLVLLALSAVAVAATPETVIAPAVKPRYRPQRIRVAAADQAGYAVAAASAFAGFAVFGLFTSLAPSFVAGSLHHPGRLLAGVVVFLVFGAAAATQAASNALSPARRFTTGVVAQAAGLIVLGAGMQLSSLMLFLVGGAIAGGGAGLLFKSAVGAVAAMAAPEVRGEALAGLFLIAYLGLIGPALGLGIATRFLSATTAMLWFTGILLALLGATAATNRALRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX29</accession>
    <name>U5VX29_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15325"/>
    <dbReference type="EnsemblBacteria" id="AGZ41347">
        <property type="protein sequence ID" value="AGZ41347"/>
        <property type="gene ID" value="AFR_15325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3112"/>
    <dbReference type="eggNOG" id="COG3371">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094600_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="228" mass="23922" checksum="872E7815079235BD" modified="2014-01-22" version="1">MTTVLIAAAAAPARPPSRPSRLLAAGLVAGPLFTAAYLVEGALRGDGYSAIRHPVSSLALEAHGWQQIVNFVVCGLLVMLFSAGLRRTLRGGPGAVVVPLLVAVWGAGLIGAGAFLTDPVGGYPTSTGPATWHGQVHDLVFSLPGFAALFLAMVTFPRRRSGRFALYSALSGITFAVFFTFATVGFAGTDPWTSTAGLWQRLCVSVGWLWLAALAALCRRMTQVHESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX64</accession>
    <name>U5VX64_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_15525</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15525"/>
    <dbReference type="EnsemblBacteria" id="AGZ41387">
        <property type="protein sequence ID" value="AGZ41387"/>
        <property type="gene ID" value="AFR_15525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3153"/>
    <dbReference type="eggNOG" id="COG0110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0663">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002751_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012728">
        <property type="entry name" value="NRPS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02353">
        <property type="entry name" value="NRPS_term_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 5" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="622"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="649"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="843"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="884"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1085"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1115"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="7">
        <location>
            <begin position="515"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="5">
        <location>
            <position position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1303" mass="136319" checksum="9627CF999608A62A" modified="2014-01-22" version="1">MTVTTDLQFVALPPIGTTDVAPAVFRSSSAPARRTLVDILVDTVRAYTGVDALDNGSTRLTYRELADEVEAVRVSLAGHGIGVGDRVGVRISSGTTELYVAILGILSAGAAYVPVDADDPEERAELVFTEAGVAAVLGDGLSVSLRRTPEGRTGRPGPDDDAWIIFTSGSTGTPKGVAVSHGAAAAFVDAEAELFLAGETAEAIGPHDRVLAGLSVAFDASCEEMWLAWRHGACLVPAERSLVRSGVDLGPWLAEQRISVVSTVPTLAALWPVEALDEVRLLIFGGEACPPELAERLAVEGREVWNTYGPTEATVVACAAPMTGQGPVRIGLPLAGWELAVVDAAGEPVAMGETGELVIGGVGLARYLDPQKDAEKFAPLPSLGWERAYRSGDIVKAEPEGLLFVGRGDEQVKLGGRRIELGEVDAALQALPDVVGAAAAVKRTAAGNQLLVGYVVPREGVDFDAAAAALRVREQLPAALVPLLAVVEALPTRTSGKVDRAALPWPLATAQDAAGELTATEAWLAGGWEEILGVRPSEAGADFFNNGGGSLSAAQLVAWIRRVHPRVSVADIYLHPRLSQLATVLDALGTTAVTRREVRPTPRRTGLFQAVLMLPMLALVGLRWLTVLAALGNVVALIVPAPWAPSVSWWWVAGGWALLFSPLGRIALAAGGARLLLRGVRPGNYPRGGSVHVRLWAAERLAELTGATAVAGAAWMLTYARALGAQVGPDVNLHSAPPVTGLLKLGRGAAIEPEVDLSGHWVDGDIVRIGKVRVGAGARVGSRSTLMPGARVGKGSRIAAGSTVSGAVPAGQRWAGSPAAPAAKDQAAWPAQRPVRSRFRSRFWALAYGVTAQLLGLVPVVAALPGLALLGWTLAYRPAAGPVLAAVAVATIAYFAGYALIVLVAVRLLSIGLRAGYHPVQGRVAWQVWATERLMGMARVGLFPLYASLFTPVWLRLLGAKVGRGVEASTVLALPAMTTVDDGAFLADDTMVATYELSHGWLRVAPARIGKQAFLGNSGMAAPGRNVPKRGLVGVLSSAPRKAKKGSSWLGAPPMPLRRTVEAADTSRTFDPPLRLKLARATIELCRIIPVMIAAALATAVLGALAVVWELAGGWMAALAAGPVMLAAALVAALIATAAKWLLVGKFRVADRALWTSFVWRNELADTFVEVLAAPWLMRFAAGTPLLTAWLRTLGAKIGRGVWLETYWLPEYDLVRLGDGATVNRGCVVQTHLFHDRVMSMDQVTLGAGAALGPHGIVLPGASIGARTTVGPGSLVTRGDAVPEDSRWLGNPIATWPAPAARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX71</accession>
    <name>U5VX71_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Beta_helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15960"/>
    <dbReference type="EnsemblBacteria" id="AGZ41474">
        <property type="protein sequence ID" value="AGZ41474"/>
        <property type="gene ID" value="AFR_15960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3239"/>
    <dbReference type="eggNOG" id="COG3147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1160036_0_0_11"/>
    <dbReference type="OrthoDB" id="1320046at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_helix" evidence="1">
        <location>
            <begin position="59"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="29249" checksum="E22CE9C52588F3AD" modified="2014-01-22" version="1">MQSGRCALPAYPTEGCTGVPRGWRPKTTKDGDLTITKRGTVLSDYLVTGTIWVKAADVTIRRTRVYGTVDNFLSDQIYGHLTIEDTEVVNPPGQEFSTNEQYAFGVANYTCRRCKVVNRMEGWRMGASSFSGSGKVTIEDSFAQLAVPPGMCASADPHGDGIQGYGGPAAVIRHNTIDQRGDDCPTAPIFIPDQDNAGGVIEDNLLAGGGYALRLTGGSFSSVTGNKIVSGSAEYGPVEVECSKVGTWKDNAVVTYDWNKGTVLEETKKLNDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX95</accession>
    <name>U5VX95_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16085"/>
    <dbReference type="EnsemblBacteria" id="AGZ41499">
        <property type="protein sequence ID" value="AGZ41499"/>
        <property type="gene ID" value="AFR_16085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3264"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="34712" checksum="596897F371AAD33B" modified="2014-01-22" version="1">MYRYGVEIEAVRTFVAVADAGQFQEAAVELGVTQQAVSKRVANLERSLGVTLFARTARGAQVTLDGQAFLPHARELLRAAERAAASVRPGERALRVDVINRRIAPAVALQNFYQAHPGIELDVVTLSDANFEIAIEAVRTGEIDATFRALPVRVKDLPGDIRVERVLDDPLQLLAGPLHPLAGARSLTPADLSGHRIWIPGIRPGTEWAAFYEELAETFDLRIDAIGPNFGTEALQDAIAGSDGLTTLVGSRDRYLWPASQDLRRIPLRDPAPVYPHSFVRRAGNPHPTLTALRRHLAATRPAGPAGAWMPSWARRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXC5</accession>
    <name>U5VXC5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">30S ribosomal protein S1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpsA</name>
        <name evidence="3" type="ORF">AFR_10455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10455"/>
    <dbReference type="EnsemblBacteria" id="AGZ40380">
        <property type="protein sequence ID" value="AGZ40380"/>
        <property type="gene ID" value="AFR_10455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2130"/>
    <dbReference type="eggNOG" id="COG0539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015805_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00681">
        <property type="entry name" value="RIBOSOMALS1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="39"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="126"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="212"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="297"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="506" mass="55781" checksum="65B95A8B66B88957" modified="2014-01-22" version="1">MTSSIEATSSANRVTVDDLGSEEAFLAAIDETIKYFNDGDIVEGTVVKVDRDEVLLDIGYKTEGVIPSRELSIKHDVDPAEVVSVGDHIEALVLTKEDKEGRLILSKKRAQYERAWGTIEKIKEDDGVVRGSVIEVVKGGLILDIGLRGFLPASLVEMRRVRDLQPYVGRELEAKIIELDKNRNNVVLSRRAWLEQTQSEVRTEFLNKLQKGQVRKGVVSSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVEVGQEVEVEVLDVDLDRERVSLSLKATQEDPWRQFARTHAINQIVPGKVTKLVPFGAFVRVDDGIEGLVHISELAERHVELPEQVVQVGSDVMVKVIDIDLERRRISLSLKQANENFVEGEEHFDPTLYGMAATYDTEGNYIYPEGFDPETGEWLEGFDKQREVWEKQYADARERWEAHTKQVQASREADAEAALNPAPAGGVTTSSSTSTSTSSSSTSSGPARAAEEPAGTLATDEALAALREKLAGGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXD3</accession>
    <name>U5VXD3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361710.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16700"/>
    <dbReference type="EnsemblBacteria" id="AGZ41618">
        <property type="protein sequence ID" value="AGZ41618"/>
        <property type="gene ID" value="AFR_16700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3386"/>
    <dbReference type="eggNOG" id="COG3911">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114480_1_0_11"/>
    <dbReference type="OrthoDB" id="1392851at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038727">
        <property type="entry name" value="NadR/Ttd14_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13521">
        <property type="entry name" value="AAA_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_28" evidence="1">
        <location>
            <begin position="3"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="191" mass="20875" checksum="558121E6AE59F1DC" modified="2014-01-22" version="1">MLRYIVSGAPGAGKTVLVRALRKLGYAVVDEAATDVIAWGQAGGDDEPWRGAGFVDAVARVQRERQQQATGVVQVFDRSPICTLALARYSDRPVTAGLAAEVERVVAQGIYQRRVFFVRLLGFITPTAARRIGLADSVRFERFHEEAYREYGFELVDVPAGTLEARVELVDGYLRAESLKEGSGRGRPCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXG5</accession>
    <name>U5VXG5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_16025</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361554.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16025"/>
    <dbReference type="EnsemblBacteria" id="AGZ41487">
        <property type="protein sequence ID" value="AGZ41487"/>
        <property type="gene ID" value="AFR_16025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3252"/>
    <dbReference type="eggNOG" id="COG1898">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090940_0_0_11"/>
    <dbReference type="OrthoDB" id="1618609at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="dTDP_sugar_isom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="PTHR21047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="4">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="22304" checksum="D653C050DE38BF34" modified="2014-01-22" version="1">MKIRQLTIEGAYEVTPVQHSDSRGTFLEWYRFDALAAAVGHPLDLAQANLSTSARDVVRGIHFADVPPGQAKYVTCVSGSVLDVVVDIRVGSPTFGLWEAVPLDDDARQAVYLSEGLGHAFCALTEGATVAYLCSSTYRPGHEHGIDPLDPELGITWPVGTPLLSTKDAGAPSLSQARAAGLLPEYAACSTYIETRRRMKDTGSSPSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYB9</accession>
    <name>U5VYB9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360764.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12095"/>
    <dbReference type="EnsemblBacteria" id="AGZ40705">
        <property type="protein sequence ID" value="AGZ40705"/>
        <property type="gene ID" value="AFR_12095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2462"/>
    <dbReference type="eggNOG" id="COG1075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_382496_0_0_11"/>
    <dbReference type="OrthoDB" id="606679at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="723" mass="78027" checksum="54C32F5412E78FA8" modified="2014-01-22" version="1">MINAYLDGGDSVTVAAGELAVALSPAAFVEPALIRAVRLEALPHLSVEAEADLWVSPLILDRTPEGLSFLPDVLPLLHQRLRSWLDEGGARALRARRAAAVILRRDAPELRLVAERVTWWALSIPDERLAAARIDDALAPVVIALQQADRGVARWVRRTLPTLPQSAHAAATVREARVRASSLTSRPIESPATAPSEPDPPRIEVAVRRRGGTLQLGAFPAKTAQGIEMPATQPLWVEVVAGGRTRVVTFRPGDTRSVDVGRGPVELRTLSGDVYRLDAVKSSAPGPADPFIGEKDIVVVIPALFGSELSRGDEIIWAGDRDTLRQLRAVRNHTAHSDGRVVPSGLLRSPLLIPGLWSLGGYRRLWTALAARAGVQEHRNLVAFTWDWRFDLQVAAQRLLETVERRLAEWRDKGGGDRESRVVLIGHGEGGLIASHYLGMLGGWDRTRLFVPIGTPFRGTLRALEFLTNGASIDPVLVDFLQGLSPLHQVVPIDPVVDLGTGILARPSDVDLPRYGVGNSRFLDEIAGPPSPPASSVVAPIVGIGQATAATAQLVSGRLRIRTNADGDSVVSIASGQPPYQVDDRRIFFAPGRQGYLPSDPRVIDYLSALLDARDVSSLGRTRTPSAPLTASIAIADHFRAGEPVTGILRAEGRSDIMLHVSEVHTDRRVLERRIVLRNDQTPFTLNLPAAPGLLLRASVVVDGRPVADADFMIVPPDPDSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYE5</accession>
    <name>U5VYE5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Methionine aminopeptidase</fullName>
            <shortName evidence="2">MAP</shortName>
            <shortName evidence="2">MetAP</shortName>
            <ecNumber evidence="2 3">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">map</name>
        <name evidence="5" type="ORF">AFR_11885</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2 3">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="2 3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360725.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11885"/>
    <dbReference type="EnsemblBacteria" id="AGZ40666">
        <property type="protein sequence ID" value="AGZ40666"/>
        <property type="gene ID" value="AFR_11885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2420"/>
    <dbReference type="eggNOG" id="COG0024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_0_1_11"/>
    <dbReference type="OrthoDB" id="1285136at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="14"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="257" mass="27278" checksum="849BF4DA23884F7B" modified="2014-01-22" version="1">MIELFDATDLPRAREAGALVAGILQTMKGRCEVGTNLLDIDRWTQDMIIEAGGVSCYVDYEPSFGRGPFGHYICTSVNDAVLHGKPFDYALADGDLLTLDLAVSLNGIVTDSAISFIVGETRSPESLALIDATERALVAGIAAARPGARIGDLSHVIGEVLSDAGYRVNTEFGGHGVGSTMHQDPHVPNTGRPGRGYTLRPGLLLALEPWIMADTDKLVTDADGWTLRSVTGGRTAHSEHTIAITEDGAEILTLPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYF7</accession>
    <name>U5VYF7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360804.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12295"/>
    <dbReference type="EnsemblBacteria" id="AGZ40745">
        <property type="protein sequence ID" value="AGZ40745"/>
        <property type="gene ID" value="AFR_12295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2497"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048587_0_0_11"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010520">
        <property type="entry name" value="FrsA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06500">
        <property type="entry name" value="FrsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="32937" checksum="774B7B57581C5AE2" modified="2014-01-22" version="1">MHITIPRGPFELAAELHLPHSVDESTPLRAVVLSTPGSSVKEQIGANYASRLAARGIAALVFDPAHQGQSGGEPRDLEDPYRRGEDISYAIDTLSALPGIDPHRIGILGICAGGGYAIHTARTDHRIKAVGAVDPGEIGASFRGFQADGPAVALDVLAEARLEETRTGVLTRENWLPDTMAEADAAGVTDIDLRQAITYYRTERGHNEHSTNRRLSRSDSLLLGYDAFHLVDQLMTQPLQLVLAEHLEGTGFDTSAERLWKLAPNPVDRLIVPGARHYQMYDVPEYVDAAVDRLAIFYENCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYJ7</accession>
    <name>U5VYJ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17895"/>
    <dbReference type="EnsemblBacteria" id="AGZ41857">
        <property type="protein sequence ID" value="AGZ41857"/>
        <property type="gene ID" value="AFR_17895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3630"/>
    <dbReference type="eggNOG" id="COG3371">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094600_1_0_11"/>
    <dbReference type="OrthoDB" id="1962568at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="19630" checksum="038E992274A8EB45" modified="2014-01-22" version="1">MIVVAGLDGATRAGYDSSRNWVSQLSLGPGGPLGVANLALCAVWLLAFGLALRHHLRLSRAGRWAARSVLVAGTAMLVVAAVPIDPGLDYPPGVPAVHTVTGYLHQAGALLLFVSGTAAAVLLGRCLSPRFPLAAPAGVAVAAVMVLAFVAASVLVTLDITGTVTGTPSGLLERVALGAGLCWIGGVGLLLSRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYS0</accession>
    <name>U5VYS0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362466.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19105"/>
    <dbReference type="EnsemblBacteria" id="AGZ42093">
        <property type="protein sequence ID" value="AGZ42093"/>
        <property type="gene ID" value="AFR_19105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3880"/>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057759_1_0_11"/>
    <dbReference type="OrthoDB" id="1690667at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="4"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="31302" checksum="80312A8ACD77E40C" modified="2014-01-22" version="1">MPTLCAVSDLHVSYAENRRIVEGLRPSSPDDWLIVAGDVAELFSSVEATLRLLRSRFAKVIWAPGNHELWTHAKDPVQLRGVERYERLVAMCREIDVLTPEDPFAVWPGEDGPVVVAPLFLLYDYSFRAPGTTTKEESLAYAYRTGVVCTDEQLLHPDPYPDRESWCRDRVAQTERRLAAIDPALPTVLVTHWPLHRHPTEVLWYPEFAQWCGTELTADWHRRFRATAAVYGHLHIPRTTWQDGVRFEEVSLGYPREWGKRPEAPALPKRILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ39</accession>
    <name>U5VZ39_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361054.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13545"/>
    <dbReference type="EnsemblBacteria" id="AGZ40995">
        <property type="protein sequence ID" value="AGZ40995"/>
        <property type="gene ID" value="AFR_13545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2750"/>
    <dbReference type="HOGENOM" id="CLU_1352218_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="202" mass="21242" checksum="5762796F1FF4820B" modified="2014-01-22" version="1">MDGDDFDGAVGGLVMLAAIIMLGACVFGADWVRPPDEGAGPFVTPEREETFGILVLVAAGICLLCVVRAIWGVTATPLVTAAIAVVVVAGFATMLYLSPTRIELRNDGFGAPTVEATVRQSVVFYNSTGTDLVVCLGVEGVCDAEASGPRKLRSPGLVVPAGYRVSVKMPVVAGDFTVTLVSDRPEIERRDMMIRSNRIQGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZJ3</accession>
    <name>U5VZJ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_13875</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13875"/>
    <dbReference type="EnsemblBacteria" id="AGZ41061">
        <property type="protein sequence ID" value="AGZ41061"/>
        <property type="gene ID" value="AFR_13875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2817"/>
    <dbReference type="eggNOG" id="COG1052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="3">
        <location>
            <begin position="5"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="4">
        <location>
            <begin position="83"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="31622" checksum="A4BA0C11F331779E" modified="2014-01-22" version="1">MPDGTLTEAELIRELADTEVYLYGGDEEATAPVIESSVALRLIAFYGVGYESFMDVEAVRRHGVRVTNTPGTLNNSVAEFTIGQLLNCRRHLTEYALKYLNGSSGDEAKQSDLGAHTVGIIGMGGIGTRLAEILTKGFDANVAYFSRTRKPALEEQLGIRYTSLNELFEVSESLLVMIPGNSETRHLVGEEQLGRLRHGSVLVNTARPEIVEPGSLDANLRSGRISAAAIDGYYSEKTEETESLKQLQPHPLLITGHVASLTHDARDAMTRRAVGSILNFLDTGADECIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W030</accession>
    <name>U5W030_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase small</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362866.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21110"/>
    <dbReference type="EnsemblBacteria" id="AGZ42494">
        <property type="protein sequence ID" value="AGZ42494"/>
        <property type="gene ID" value="AFR_21110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4279"/>
    <dbReference type="eggNOG" id="COG2890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056435_0_0_11"/>
    <dbReference type="OrthoDB" id="2049098at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="2">
        <location>
            <begin position="39"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="22090" checksum="2E3C63099DB4C62B" modified="2014-01-22" version="1">MSDKSFWDSRYAESHHVWSGNPNVVLVREVEGLTPGSALDLGCGEGGDAIWLAARGWAVTAADISAVALEKAAAHAAEAGVTVDFQQHDLAVSFPSGSYDLVSAQFLYSRENLPREQVLARAARAVAPGGILLIEGHQDFGPFAAQSQDHGDVHFAEPGEVVAALELPAGEWEVLLCEKHDRLQNGPDGRPAHRTDSTVKVRRLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0E8</accession>
    <name>U5W0E8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363064.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22115"/>
    <dbReference type="EnsemblBacteria" id="AGZ42693">
        <property type="protein sequence ID" value="AGZ42693"/>
        <property type="gene ID" value="AFR_22115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4483"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108054_2_1_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="135" mass="14392" checksum="24D3069FA354411C" modified="2014-01-22" version="1">MPLGRLHHTIVDCPDPLALATFYSHLLGTPITYRDDDFAVVSTSDRASGLAFQRAPDHRAPTWPDPAVPQQIHLDVMVEDVAAASAAVIALGATPLPGTGVFADPAGHPFCLIPRPHWAPPIPLGQSPDEDHRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0G0</accession>
    <name>U5W0G0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362951.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21545"/>
    <dbReference type="EnsemblBacteria" id="AGZ42579">
        <property type="protein sequence ID" value="AGZ42579"/>
        <property type="gene ID" value="AFR_21545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4369"/>
    <dbReference type="eggNOG" id="COG0631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067299_0_0_11"/>
    <dbReference type="OrthoDB" id="1359757at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="27713" checksum="2BB91D5098838227" modified="2014-01-22" version="1">MRFTIDSVPKRPGRTNEDFAGVVPGAAVVIDGAGIPGTEEICRHGVAWYAHRLGGTLLGLLPLDGSLTTLLAEAIEQVTDAHRDTCDVASPISPSATVALLRVREGTAEHLVLGDTTVVLDRHDAAPLVVTDAREVVISRTYEARLKDVPAGSDDYRRILAELRTHRNQPGGFWLAKDDPRAAAQARTGSCPVAGLRGAALLSNGAGRFVDRFGLSDWPGLLAILTTTGPDEVIRRVREAEAHENVAPDDATIVHCTALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0H7</accession>
    <name>U5W0H7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative RNA-binding protein containing a Zn-ribbon-like motif</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361495.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15770"/>
    <dbReference type="EnsemblBacteria" id="AGZ41436">
        <property type="protein sequence ID" value="AGZ41436"/>
        <property type="gene ID" value="AFR_15770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15770"/>
    <dbReference type="eggNOG" id="COG5516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087298_0_0_11"/>
    <dbReference type="OrthoDB" id="1478254at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010852">
        <property type="entry name" value="ABATE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023286">
        <property type="entry name" value="ABATE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021005">
        <property type="entry name" value="Znf_CGNR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35525">
        <property type="entry name" value="PTHR35525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07336">
        <property type="entry name" value="ABATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11706">
        <property type="entry name" value="zf-CGNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160904">
        <property type="entry name" value="SSF160904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-CGNR" evidence="1">
        <location>
            <begin position="137"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="187" mass="20423" checksum="C106D19A7A0F0BD7" modified="2014-01-22" version="1">MSSEIEVPPAARLVRDFVNTAEPQVAGEVLTSPERLAGWFAEQRLVDPGTRLGPADLTAAVSIREGLRSVLLGHAGHSADPDALERLTGVLATVPLRPVFSVDGYRLAATGGTAFDGACAQLVDAIRRCREDGTWGRLKVCARETCRWAYYDASRNQVRRWCSMEGCGNHVKTKRAYAVRKQRAAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Z6</accession>
    <name>U5W0Z6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidoglycan-binding domain 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_16735</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16735"/>
    <dbReference type="EnsemblBacteria" id="AGZ41625">
        <property type="protein sequence ID" value="AGZ41625"/>
        <property type="gene ID" value="AFR_16735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3393"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033064_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="3">
        <location>
            <begin position="164"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="481" mass="49767" checksum="EDF12317F1C288CB" modified="2014-01-22" version="1">MFGFAWDRPDGEQILEEPAAAPAPAPREDGPVRRNGRRRWALLMAGGATLLSLGGLIGASFVQSPEQQRASALPPAPSVLTATVEERLLASTVVTRGEVGADRQVEVTPASAQGAEVTVVTAVRTRVGAKIKPGDVVLTVSGRPLMILPGAIPAYRDLKPGDEGDDIAQLQSALRRLGHYDSGDRRGWFGPSTKAAVRRLYADIGYEVPDTGGPGGQEDREPLRAADDAVGAARSAVTSLKARLAAATPPPSAPGEPTLKDQLKAAKRTLARAEQDRRDLVARTGPMVPLAEAAFLTTFPATVVAVGRKVGDPVEAPLITFATGRLTVTAKLQPEQASLLRPGMPVEILSETLREKATGVVGTVGKLTTDRSGGDGEEGQAAGLPYVPVTVTTKKALGNQWNNLDVRLTVTAAQTSGKVLVVPVSAVSASADGTTTVSVSRPSGDLVRVSVEPGVSGDGFIAVTPAADATLAAGDRVVVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W173</accession>
    <name>U5W173_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_22460</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363152.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22460"/>
    <dbReference type="EnsemblBacteria" id="AGZ42762">
        <property type="protein sequence ID" value="AGZ42762"/>
        <property type="gene ID" value="AFR_22460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4553"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="335" mass="35087" checksum="2E0134F13FFD8EBA" modified="2014-01-22" version="1">MPTISDVARAAGVSMSTVSYVLSGRRPISAETQARVKAAIAELGYHPHAGARALASSKTSVLALVVPLRVDVSVPVIMQFVTAVVTAARTYNHDVLLLTKDEGTAGLERVAGATMVDALIVMDVERDDVRIPALGRLKQPSVLIGLPDDAAGIACVDLDFAATAAECLSHLAGHGHRRIALVGPSPAVYERGTTYAPRFLSGFTEAATDLGLSTVAHPCEPGAEGVRECLAQIDAELPGTTALVVHNEEALLPLLGELRASGRRVPEDLSIVAVCPRDVAVGLPVRLTSADIPAHDVGTLAVETAMNLLDGRRTPQTRLLPPTIVERDSCAAPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1J8</accession>
    <name>U5W1J8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363614.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23780"/>
    <dbReference type="EnsemblBacteria" id="AGZ43024">
        <property type="protein sequence ID" value="AGZ43024"/>
        <property type="gene ID" value="AFR_23780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4817"/>
    <dbReference type="HOGENOM" id="CLU_2505303_0_0_11"/>
    <dbReference type="OrthoDB" id="2083262at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="85" mass="9246" checksum="8D06D89A7316050F" modified="2014-01-22" version="1">MQPRPPRLFEADDVLSGRISLDGYPFRLIYLVISAASFYAGTSIHPGDLGRVDVLLSAAELLETRGWQTVSVDAGGKLLCLRRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1T5</accession>
    <name>U5W1T5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363773.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24585"/>
    <dbReference type="EnsemblBacteria" id="AGZ43183">
        <property type="protein sequence ID" value="AGZ43183"/>
        <property type="gene ID" value="AFR_24585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4981"/>
    <dbReference type="eggNOG" id="COG5579">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124534_0_0_11"/>
    <dbReference type="OrthoDB" id="1625285at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014937">
        <property type="entry name" value="DUF1810"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036287">
        <property type="entry name" value="Rv1873-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08837">
        <property type="entry name" value="DUF1810"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008546">
        <property type="entry name" value="UCP008546"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140736">
        <property type="entry name" value="SSF140736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="136" mass="15202" checksum="C08817CDE5D87DD2" modified="2014-01-22" version="1">MSDLARFVDAQQDVHERALAELRAGRKRSHWMWFVFPQIAGLGLSATAQRYAIAGLAEAREYLAHPVLGPRLVESAETLLGVEGRTAEQIFGYPDDLKLRSSMTLFAHAAEDPAVFRAVLDRYYDGEDDPATLQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>U5W217</accession>
    <name>U5W217_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Non-ribosomal peptide synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18490</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362349.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18490"/>
    <dbReference type="EnsemblBacteria" id="AGZ41976">
        <property type="protein sequence ID" value="AGZ41976"/>
        <property type="gene ID" value="AFR_18490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3752"/>
    <dbReference type="eggNOG" id="COG1020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_2_13_11"/>
    <dbReference type="OrthoDB" id="572620at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="3">
        <location>
            <begin position="721"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="1">
        <location>
            <position position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1062" mass="113795" checksum="AB287639BCF8AFA0" modified="2014-01-22" version="1">MTSAAELTGTAAPRWTGAGNTATGPGLQRVRLPDDLVRALRSSGADLASVLLAVHARVLATVTAEHEVQFGLVAPGSAAQSGRRLEVTKSTWRELTAQARAAVAIAGDCEVVLDLSALDGDPAAPAGGEPLRLSYAGGDDLHLTVRHDRAVLDDTYAQRLAGYHLTALRAYAEDPDAPHHRRSLLSDAEMETQLYHLAGRRAELPRTTFPEVFEEQVQAGPNRVAASHGTATLTYEELNARANRIAHALLGAGLTAEAPVAVVLDRTLDWLAAAIGVFKAGGVYLPVRPDFPADRIAAQLARSECRLVLAEPGSEDLAHRAVAVAGADAQVLSVPEVLAGDAATTDPGVPVAPEQAAYIYFTSGSTGTPKGALCEHAGMLNHLLMKLEDMGMTGREGEVVTQTASQCFDISLWQFAAPLMAGGSVRIVDTDVLLSVGGFLDELVGNNVTVAQIVPSYLEVVLTHLEQWPRPLGRLRSLSVTGEALKLELVQRWFAAYPDIALVNAYGATEVSDDTMHEILDGLPDRDFVTVGTSRRNINTYIVDENLALVPLGSPGEIAFSGVCVGRGYINDEERTRQAFVADPYRSGTRMYRTGDFGRWLPEGRIEFLGRRDEQVKIRGFRIEIGEIENKLLTFPGVRESAVVIDDGAGEMRNLVAFYSGDGVEPEHLRDFLAGLLPEYMVPTYFHGLQQLPLTENLKVDKKVLKRLAGTLGHGGATYVAPTTPTEQRLATLWAEVLGVPLERLGRDDDFFELGGTSLAAVRMLISMNGTLSLKQLVGNPRLGELATVLDGRETAQTRSGLVQPLSTVTNPHHALVCFPYAGGNAVNFRALATALRHDGIAVYGVELPGHDLAADREPMADVPQIAARVAAEIRDQLRIPVLLWGHCAGAAAALATARLLEQAGAPAERVFLGALLVESAGELRRESAEAEAADNPTLLARLRAESAYVELDGLQPERLDIVGSSYRHDVVTANRYLLDLLAEPHRHRIEAPVHVVAAADDPTTPGHETRYADWKLVADRVELHEVADGGHYFAGSRPEATAELVRAACAVRQPAPVGGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2C8</accession>
    <name>U5W2C8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase U61 LD-carboxypeptidase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_25115</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S66 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25115"/>
    <dbReference type="EnsemblBacteria" id="AGZ43289">
        <property type="protein sequence ID" value="AGZ43289"/>
        <property type="gene ID" value="AFR_25115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5089"/>
    <dbReference type="eggNOG" id="COG1619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034346_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027461">
        <property type="entry name" value="Carboxypeptidase_A_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027478">
        <property type="entry name" value="LdcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040449">
        <property type="entry name" value="Peptidase_S66_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040921">
        <property type="entry name" value="Peptidase_S66C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003507">
        <property type="entry name" value="S66_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30237">
        <property type="entry name" value="PTHR30237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02016">
        <property type="entry name" value="Peptidase_S66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17676">
        <property type="entry name" value="Peptidase_S66C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028757">
        <property type="entry name" value="LD-carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141986">
        <property type="entry name" value="SSF141986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666002" evidence="4">
        <location>
            <begin position="31"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S66" evidence="5">
        <location>
            <begin position="47"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S66C" evidence="6">
        <location>
            <begin position="210"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR028757-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="35467" checksum="F6AB31B800FDA026" modified="2014-01-22" version="1">MTLTRRTLLGATVAGGGTLVAGATAAPAQAAPRRAVKPPMLRPGDRVRVIATAYQPDSRLDRGIEILESFGLEVEVGDHVDDVYGYLAGTDDDRLADLNAAFSDPGIRGVFAARGGYGTQRIVDRLDLEAVRRDPRVFVGFSDLTSLMGRLWQSARLVTFYGPMVNWTDSRTGPVEIESLRKAIMTTEPAVLTRDPAEPSSAVVVPGTATGRLLGGNLTMIQTAIGTRDLPDLAGAILLFEDVDEAAYSYDRMLTQLLRTGALDRIAGVAIGQFTNAANNATWNAATAVLDRIGGLGVPVLGGLRIGHGNGQLTVPLGAKATIDTAAGTLTVESGVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2H2</accession>
    <name>U5W2H2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_25845</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25845"/>
    <dbReference type="EnsemblBacteria" id="AGZ43433">
        <property type="protein sequence ID" value="AGZ43433"/>
        <property type="gene ID" value="AFR_25845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5239"/>
    <dbReference type="eggNOG" id="COG0443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010904_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="370"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="780"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1023"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="375"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="500"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="547"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="614"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="697"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="783"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1043" mass="106997" checksum="657C54DB4E272E61" modified="2014-01-22" version="1">MAMQYGLGVDLGTTQTAAAVNVDGRIEVVRLGGRRAEIPSLVFVNSDGSLLIGDAAERRGQAEPGRLAREFKRRIGDPVPILVGGVPFSAHALTAKLLRHVIDTVARLHGEPPAVLTVTHPANWGPYKREQLDQALRLADTGPVLLRTEPEAAALQHATARKIASGETVAVYDLGGGTFDAAVLRRDGEGFQLLGDPEGIEQLGGADFDEAVFGHVLGMLGTTIEGLDPNEPDVVTALARLRRDCVEAKEALSFDTEVMIPVALPGIHTRVRLNRSELESMIAPALEDTVAAMHRALRAAGVSPDELDAVLLAGGSSRIPLVSQLLSTAFDRPVIADPHPEHSIAMGAAVSTALITGGASPFAAAVEPAATNGSAPAESDHTTPATSAAANGAAASGGPAANGGPATSASPAGSGSPAQDGGTAAGSSPAHDPASATSAAAGGPADNGSASQRGPATAPFPGPETDPLPATFTAAFPGGTFTGPLTGPVTGPMDAPTGATAGASNASRGPETQAATNGSASNRPGNSNRPGNQGAAGRPPAQPAPVASAWAQSTGDQSETLITPSDPWADDHVERPEDQAAADWYHAQSAAAAGPATETAPVSGAGASQADDRHWSEGTATQAGSPAPQDQPWHESQAPQEGGHRQGNPAPTQYMPGAGGHNAGAVAFSQGAASIPGTGPAQTAPGGRTQVFSPPAPAGSPGGPTSYPPAPPRLDDDPPKRNGRRLILVGAITLAVIAGTTAAVIALNQGDKKGNTGAQPSAGPSVAPPRFPTETMLIRVDTGGPDRPERKSSVKLVTPGRPDRTLISDTGNDVLPEWSRDRKKIALTRNLADGSNEIWVMDADGKNARKVIGDVTGGRTTWSADGTKLAFMRTVGEQAQIFIIKIGESEPRQLTSAKTVKDDPAWSIDGKSIAYWAEIDGERQIYLVNVDNPEEPGKQITRGDEGPAYDPAWSPDGKTIAYTHGTGPGISDIWLVDSDGTDAHELTTDDEREMDPSWAPGGEWLAFTRGPLDKPRIVVTTKDGGDEETLTQGTAREGHPCWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2K0</accession>
    <name>U5W2K0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutathione S-transferase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19665"/>
    <dbReference type="EnsemblBacteria" id="AGZ42205">
        <property type="protein sequence ID" value="AGZ42205"/>
        <property type="gene ID" value="AFR_19665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3991"/>
    <dbReference type="eggNOG" id="COG0435">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037263_1_3_11"/>
    <dbReference type="OrthoDB" id="780709at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="PTHR32419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="143"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="31061" checksum="2E93D06C4BBE9E94" modified="2014-01-22" version="1">MMLASPVDTATHGEYAIPDTPGPLLRFTGRITGDARPFRYHVYGGWFCPWSQRVAITRALSGLEDVVGMSFVDGDRDGRGWAFRERYGPDPVNGFTLLREAYDATEENFDGHVSVPALWDRENARLVSNDCATIGIDLATRFRHLATPVVDTYPERHRERIEELDRWLGPAVNHGAGAAARPGAARSALLEAFEQLDEHLSGSRYLIGDDLTEADIRLWVTLVRYDVGVNAGRTVSPGLHVHPHLWSYARALYRIPAFRDTTDFAAFSRPGAVRPDWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2X5</accession>
    <name>U5W2X5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362717.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20365"/>
    <dbReference type="EnsemblBacteria" id="AGZ42345">
        <property type="protein sequence ID" value="AGZ42345"/>
        <property type="gene ID" value="AFR_20365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4129"/>
    <dbReference type="eggNOG" id="COG5552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170430_0_0_11"/>
    <dbReference type="OrthoDB" id="1811614at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018735">
        <property type="entry name" value="DUF2277"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10041">
        <property type="entry name" value="DUF2277"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="89" mass="9676" checksum="F1C8E598FFB728BB" modified="2014-01-22" version="1">MCRNIRQLHNFEPPATPDEVQAAALQYVRKVAGSTKPSQRNQEAFDRAVAAVAAATAELLEALVTTAPPKDREIEAAKAKERAAQRYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W375</accession>
    <name>U5W375_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362822.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20890"/>
    <dbReference type="EnsemblBacteria" id="AGZ42450">
        <property type="protein sequence ID" value="AGZ42450"/>
        <property type="gene ID" value="AFR_20890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4235"/>
    <dbReference type="eggNOG" id="COG5343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082046_0_0_11"/>
    <dbReference type="OrthoDB" id="1972141at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018764">
        <property type="entry name" value="Anti-sigma_K_RskA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10099">
        <property type="entry name" value="RskA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="232" mass="24435" checksum="25CA46586CB0D062" modified="2014-01-22" version="1">MQHLEPDRLVLLALSEDTPAADESVHLAGCPGCSAEIDDLRHVAELGSETQGLDSLPPPPERVWDAIAAATTAPVRRLPVRRERRWALPVLAAAAAAVLAIAGTVVVQRVVERSAPPDVTAKASLVRLDTAPAAAHGAAEVIGGTRLRVDVRNLPLNPGYYEVWLIDPEDTTKMMAMGNIDGAALTGAADVVLPLPPGADLNRYRLVDVSSEAYDGNTAHSGKSLLRGTLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3A5</accession>
    <name>U5W3A5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase GCN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560026.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27135"/>
    <dbReference type="EnsemblBacteria" id="AGZ43688">
        <property type="protein sequence ID" value="AGZ43688"/>
        <property type="gene ID" value="AFR_27135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5499"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_6_11"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="20463" checksum="3F9094DA4BD09131" modified="2014-01-22" version="1">MLITTERLVLRRFRAGDAETLAAYRSSPDVALYQSWDAPYPLERARYAIESMAEADPAQPGWFQYAIETKAGKTHIGDVGVNLNDNLLQAEIGYTVAPEHQGNGYATEAVRAVLNHLFRVQGLHKVAAECDARNEASARLLERVGFTREGHLRQHTWIKNEWTDDLLYGLLEVDFFGLDTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3C5</accession>
    <name>U5W3C5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phenylacetic acid degradation protein paaC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362808.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20820"/>
    <dbReference type="EnsemblBacteria" id="AGZ42436">
        <property type="protein sequence ID" value="AGZ42436"/>
        <property type="gene ID" value="AFR_20820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4221"/>
    <dbReference type="eggNOG" id="COG3396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070585_0_0_11"/>
    <dbReference type="OrthoDB" id="1389523at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007814">
        <property type="entry name" value="PaaA_PaaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011882">
        <property type="entry name" value="PaaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05138">
        <property type="entry name" value="PaaA_PaaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037834">
        <property type="entry name" value="PA_CoA_Oase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02158">
        <property type="entry name" value="PA_CoA_Oxy3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="31851" checksum="218AC825B1D764A7" modified="2014-01-22" version="1">MSFDAAYEALTDHDDDARWAYGTGFADPLAGVDTAVPAAVDRADLTAYCLMLGDDALIMSHRLQQWVTRAPELEDELAIANIALDLLGQARLLLTRAAEVEDAGRDEDSLAYLRDPADFRNVRLVERPDADFAHLVARLLVFATWRLALLDRLTGSADPVLAAIGAKGVKEVTYHRDYAAQWVVRLGDGTEVSHRRMQVATDAVHLLTDELFRPHEVELRLAEAGVAVDPSALRAEFEAVWAQVLEAATLRPATDEVPAEQHGRNGQHSSELAEILTELQGLARSTPGGVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3P6</accession>
    <name>U5W3P6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362967.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21625"/>
    <dbReference type="EnsemblBacteria" id="AGZ42595">
        <property type="protein sequence ID" value="AGZ42595"/>
        <property type="gene ID" value="AFR_21625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4384"/>
    <dbReference type="eggNOG" id="COG0412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112481_1_0_11"/>
    <dbReference type="OrthoDB" id="1889569at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DLH" evidence="1">
        <location>
            <begin position="4"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="19663" checksum="E23596E8E34B2770" modified="2014-01-22" version="1">MTDVVLFHHAQGLTAGVHAFADQLRSAGHTVTVPDLYAGVTFDNLDAGVAHAQEIGMDEIVDRGVAAVAGVPDAVVYAGFSLGAMPAQKLAQTRKGALGAIIYHGGVPAEAFGGEWPKEVPLQLHLAEEDEWAEVDVARDLAAASTDGTLFLYPGAAHLTTDSSLGEYQPETTALILGRSLEFLARVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3X2</accession>
    <name>U5W3X2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22050"/>
    <dbReference type="EnsemblBacteria" id="AGZ42680">
        <property type="protein sequence ID" value="AGZ42680"/>
        <property type="gene ID" value="AFR_22050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4469"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058409_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="308" mass="32432" checksum="D4ADAAFA05F4B484" modified="2014-01-22" version="1">MQDDFSKQVAGLPLPGSGQTAERWWRLASLGRDDLVLARLAEGHADATAIRAELKDEPSADPDSERWGVWAAVPSSLEARRTEDGAWSVSGDRPWCSGAGSCTHALVTAVAEDGVRLFAVEVREPAATPLDDTWPAVGMAGSDSRTVRFADCPARPVGGPGDYVGRPGFWHGGVGVAACWYGGATGVADTLLAAARTRDLNPHALAHLGAVDGLLHTARLALRDAAERIDAEPSADAQQLAVRVRTVVDSVATQVLDHVGRALGAGPLCQDAAHARRVADLTVYLRQSHAEADLAQLGTLLKENPPAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W430</accession>
    <name>U5W430_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TAP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363037.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21980"/>
    <dbReference type="EnsemblBacteria" id="AGZ42666">
        <property type="protein sequence ID" value="AGZ42666"/>
        <property type="gene ID" value="AFR_21980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4456"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013364_3_1_11"/>
    <dbReference type="OrthoDB" id="665798at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013595">
        <property type="entry name" value="Pept_S33_TAP-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08386">
        <property type="entry name" value="Abhydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665671" evidence="2">
        <location>
            <begin position="26"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="97"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Abhydrolase_4" evidence="4">
        <location>
            <begin position="411"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="565" mass="60423" checksum="DAF85C999AEE5527" modified="2014-01-22" version="1">MRFRSRLLAAGLAGLLLSGSVVAPAVARERADATTSTAEARRVGSVRTPDLHWYACYGWAQCATAKVPLDYDHPYGTQTTVALLRVKAKDQKNRIGSLFVNPGGPGGSATTMALAAPFFLSASLLQRFDIVGMDPRGIGASDNVACFADTGKQTAALKGMNVAFPVTAKETKAYLNGARKLATGCSTTGRSLAGAMSTAEVARDMDVMRRAVGDKKLTYLGFSYGTALGQYYANMFPDRFRALAVDGVINPRSWVGSEASKNVIQDERLHSADGAYKALIRVLKLCDKAGETRCAFAAGDPVQRYATLAGRLKARPVTVDGFKITYAEFVGLTLDALYGVDAGADVTMLAAELWKLTSTPKAATASSVTGRAFPYDNSSDAYAAVMCTDGLHPGKAANWPAKAARADKRAPYFGRLWAWQSVQCARDIWKVRDEDAYTGPFTKRTRNTVLVVGSYWDPATNYYEAVSSAAKLPNSRLLSSDNFGHTAYGTSTCATRAMDHYLLYKALPARGTVCKGEQPFMHKLSDGTKEAAPGLDLATATKAEIVAEGLPAEGEPQLLPPVGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W433</accession>
    <name>U5W433_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28165"/>
    <dbReference type="EnsemblBacteria" id="AGZ43894">
        <property type="protein sequence ID" value="AGZ43894"/>
        <property type="gene ID" value="AFR_28165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5709"/>
    <dbReference type="eggNOG" id="ENOG5032C0P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_927215_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="28067" checksum="3B3774CEEF49391A" modified="2014-01-22" version="1">MVMIANAYLMTRGTPRRNDYAFLGAVPPSRWWDRVNPYVFMESPEVVVARHPGGVGVLISGLPSARRDVIGTAIRHTVVIDDLRDQPELLCRIVAAGLDPRTRTALGEHLDEEFDAGRVDAIMDGGDAGTDVPQRVAEILATVSADVPGPGRDPEGSWVGGADDEGSRRAFLARVRRLAAEEASGFAFTSQAIATARGAGEAAGRLGAPVAILLVDGDVDGVQPLGKDRPLPPERRSPALLMTGLALTGVAVAVILWWIARRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Q8</accession>
    <name>U5W4Q8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560520.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29610"/>
    <dbReference type="EnsemblBacteria" id="AGZ44183">
        <property type="protein sequence ID" value="AGZ44183"/>
        <property type="gene ID" value="AFR_29610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6002"/>
    <dbReference type="eggNOG" id="ENOG503381P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092083_0_0_11"/>
    <dbReference type="OrthoDB" id="1853708at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="24915" checksum="C3BA8D416D153C0E" modified="2014-01-22" version="1">MPLLAGAVDVDAVMASLSSARRVFHSEADFQHAFAWVLHEVEPSLNIRLEVRQDKREYVDLFCFGPKGRTAVEFKYFTARWDGTDSATEKFHLRGHAATDLARRNFVFDIARLERFCASGRATNGFAIMVSNDRRLWEPIVSRRVARDQEFRIDEGRALKDVLRWGTEGSYFDANQRELAGSYSVAWRDFSELNGPAGDFHQLILPVEQRAQGVTRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W559</accession>
    <name>U5W559_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_30120</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560621.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30120"/>
    <dbReference type="EnsemblBacteria" id="AGZ44284">
        <property type="protein sequence ID" value="AGZ44284"/>
        <property type="gene ID" value="AFR_30120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6101"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_6_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="57"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="33282" checksum="AD86E3D2C8E75E0B" modified="2014-01-22" version="1">MTLFWRIFSLNAAVLAVATVGLLLGPVTVSTPVVLTEAVVLIGGLAAMLVANAVLLRIGLAPLARLTRTMTTIDLLKPGTRLPVTGRAGIAALIRAFNAMLDRLETERATSAARALSAQEAERKRVAQELHDEIGQTLTAVLLELKRVADQSPPPVRDQLRQVQETTRNSLDEIRRIARRLRPGVLEELGLTSALRALTTEFSTAGLAVQRQIGPDLPTLSEDVELVLYRVAQEGLTNVARHADARHAALILRREGDGVELRIRDDGRGVGTASEGAGIHGMRERALLIGAALTVAPGPEGGTDVSLRVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5A8</accession>
    <name>U5W5A8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_24115</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24115"/>
    <dbReference type="EnsemblBacteria" id="AGZ43091">
        <property type="protein sequence ID" value="AGZ43091"/>
        <property type="gene ID" value="AFR_24115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4885"/>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029843_0_0_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006189">
        <property type="entry name" value="CHASE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042240">
        <property type="entry name" value="CHASE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03924">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01079">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50839">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="CHASE" evidence="8">
        <location>
            <begin position="141"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="378"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="603" mass="64528" checksum="866E1E317EEC270A" modified="2014-01-22" version="1">MPGAGGWAPGRWSVRVLALVIALGVLGTVLAAVALRRADDDRARRALGQQTMIVSQVVSAEVRRYSTSLTDLAAAVGAQANLEAAEYTAIAASVDRQRLPGASGVAFVVAATTDEMPAVQKSWRDRGDPQLTFTPSSAPTDEHFFVVLYRGVEGGPSPLGLDVADKPEAVEAMQRAKASHRVATSSTYRLLTDSELPVERQQLSFVLAAPVYSTSPSASDTGQFRGWLMMGLRGGDFLKQAIGVVARDTVAVTLYDTASTAGPTPVASWVPDARLQPEPPPRDITVSVPQRTWRLVVEPTHKLLPVGDMHLDVAAWIIGAVLTGLLAALTGTVITARNRALRKVDDATAALRDDIVRREAVEQQLRQREAELVGFAGIVAHDLRSPLARITGYADFLREEAAPRLDPLHRDFLERLYGGAQRMQTLIDDLLDYATAENRTLHTSRVDLRAMADDITRERLTGTEAHPPTIVIEDLPTVDGDPTLLCQVLDNLIGNALKYTAPGEEPYVRVSCRTTDGMARIEVADHGIGIPADQRDTVFTAFARAPGSAGYPGTGLGLAIVHRIVERHGGEVGVDANPGGGSLFWFTVPHAAALTPSVTAPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-02-10" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5G8</accession>
    <name>U5W5G8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription antitermination protein NusB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Antitermination factor NusB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">nusB</name>
        <name evidence="8" type="ORF">AFR_30645</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the NusB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30645"/>
    <dbReference type="EnsemblBacteria" id="AGZ44389">
        <property type="protein sequence ID" value="AGZ44389"/>
        <property type="gene ID" value="AFR_30645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6205"/>
    <dbReference type="eggNOG" id="COG0781">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087843_2_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00073">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011605">
        <property type="entry name" value="NusB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11078">
        <property type="entry name" value="PTHR11078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01951">
        <property type="entry name" value="nusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusB" evidence="7">
        <location>
            <begin position="5"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="136" mass="15503" checksum="2202E98E06AEC963" modified="2014-01-22" version="1">MPARRKARKRALDVLYEADLRDLPPAQVLVTYLDRIEKPRPDHLDYTINLIEGVAKHLDRIDELIASYAEGWTIDRMPVVDRNLARIAVYELLYVGEIDDPVAISEAVELAKQMSTDDSPRFLNGILGRIAEFATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W620</accession>
    <name>U5W620_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein icfG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31815"/>
    <dbReference type="EnsemblBacteria" id="AGZ44623">
        <property type="protein sequence ID" value="AGZ44623"/>
        <property type="gene ID" value="AFR_31815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6440"/>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043255_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="162"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="379" mass="41224" checksum="74A129D3D099EFC7" modified="2014-01-22" version="1">MSDRPTPVGALLRAAPPDRLPEVVADHLRRHYAAGRTEVLLGDLMLSSLWPVLDRDGPSGNTLALRCFGSQRPVVDIAHDANRVYLPLTAWGERLGVLVVQTTLPSGTGLTDELSVIADEFAVALRAADVVTDRYRQVTRRERLTMAAELQWEMLPGRSLGDDRFLVAGQLQPAYAVFGDHYDWSLIGDRLTLTVLNGHGDGLDAAVLTTVAVNAMRNARRSGANIVEQAELASDALYSRYGGAAHAATLLLEIDLVGGRVEAVDAGSPRAVLAREREIRPVTLEQQLPLGMFGETRYETQRFLLEPADRLLVVSDGVHAASPGGRAPYGESALLTALRRTRLQPATEAVGTVMRGLREYHAGTEPEDDAVTVCLDWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W633</accession>
    <name>U5W633_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulatory protein desR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_25735</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364087.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25735"/>
    <dbReference type="EnsemblBacteria" id="AGZ43411">
        <property type="protein sequence ID" value="AGZ43411"/>
        <property type="gene ID" value="AFR_25735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5217"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="144"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="22448" checksum="AFC8973962258E6E" modified="2014-01-22" version="1">MIRVMIADDQAMVRTGFGMIIGAQPDMELVGEAADGVEAVALARKIKPDVALFDIRMPRMDGLEALRLLAGPGVADPIKVVVVTTFDLDEYVHQALRNGAAGFLLKDSGPALLIEAVRAAVSGDALISPSITVRLLEHLSPPAPTGDDGGLSPRELDVVKLAARGLTNAEIATQLFISVGTVKTHLGSVQTKLGARNRVEIAAWAWERRLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W655</accession>
    <name>U5W655_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mandelate racemase/muconate lactonizing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_26115</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26115"/>
    <dbReference type="EnsemblBacteria" id="AGZ43485">
        <property type="protein sequence ID" value="AGZ43485"/>
        <property type="gene ID" value="AFR_26115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5293"/>
    <dbReference type="eggNOG" id="COG4948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_9_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03323">
        <property type="entry name" value="D-glucarate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034598">
        <property type="entry name" value="GlucD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="Mandelate_racemase_DgoD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="PTHR48080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MR_MLE" evidence="4">
        <location>
            <begin position="162"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="212"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="311"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR634598-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR634598-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="413" mass="43569" checksum="E4DA4797CFCEA32D" modified="2014-01-22" version="1">MAVTPIAFPDPPLLNSAGVHEPWALRAIVEIRCGDGLTGVGETYGDAPHLALLAAAARSLTGLDVFDLAGLRSRVAAAIGTVDTPDRHGLTGGSSAAKTLARVVSPFEVALLDLQGKAIGRPVHALLGGKVRDTVPFSAYLFYKWAGHPGAEPDEYGEALDPAGIVAQARKLVDRYGFRSIKLKGGVFPPDEEIAAIRALRAEFPGLPLRLDPNCAWTVGTSVKVAEETAGLLEYLEDPTGGLAGMAEVAALAPMPLATNMCVVQFADLPESVARGSVGIVLADHHYWGGLSASARLATICDTWGLGLSMHSNSHLGISLAAMTHLAAATPNLTYAVDTHLPWQLGVDVVDSPLTFVDGDVVVPDAPGLGVAVDPDALARLHENYVACGITERDDTAYRRRFEPGYEKLRPRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6D2</accession>
    <name>U5W6D2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger SWIM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561031.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32205"/>
    <dbReference type="EnsemblBacteria" id="AGZ44699">
        <property type="protein sequence ID" value="AGZ44699"/>
        <property type="gene ID" value="AFR_32205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6519"/>
    <dbReference type="eggNOG" id="COG4715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_308320_0_0_11"/>
    <dbReference type="OrthoDB" id="1115894at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043746">
        <property type="entry name" value="DUF5691"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18944">
        <property type="entry name" value="DUF5691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="55"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="954" mass="101636" checksum="82F07D661F578E8A" modified="2014-01-22" version="1">MPVERWSTAQITALAPDASSLKGARGVSSASKWQEAGLLDEVLWGLCRGSGKNPYQVCVDLAGPVYKCSCPSRKFPCKHALGLLLHWAEGGGFTAPAPAFVSEWQASRAAKVAAPKTTGTADPAAAQKRALQRADRVAAGMTELRRWLDDQVHQGLAGAEQAGHRPYETMAARLVDAQAPTAASAVRRLGGVAGIGPHWADRLLGDLALLRLLVAGHERLDTLPDALAATVRARIGFPVATEDVLATTPVRDEWQVLGQVDSEDGAITTRRTWLLGVSGGRFALVLSFAAPGQSLTADLLPGTTFDGALCFYPGAAPLRALVKEKFGTPRPLTTPSGARGLRDALAGWSTTVAAEPWRYDAPVLLAGVVPGPDGFLVDAAGESLPLAPGHREPWWLLAAAGGRPATVAGEWSPSGLRPLAAWVRGQFLPAAAPVPDGPSVRGPELPADLLAAALVGTARRPWSSPDIHVGDRVLTMSKGGELLEAAGAALVYRRAGITPSAGHAPVEAAPVEIDRPLPEAAGARLLRLLTDGGAPGGAQQAQELLSQWLFAAAQRGGFVPPESLPALLDAGRRNSAVRPAIGRIAGQRGTWLARMRPDWKWLSDEAPGTATSDDPEIWETGTGGERLAYLTKLRGTDPGAALALLESTWKTEAPEDRARFLGALDIGLSPADDTFLDRALDDRRKEVRETALDLLQRLPGSSLGQRMAARATAAVRLERRTFGRDRLLADPPTELTPDLRRDGVAAQPSRGTGVQAWLLEEIIAGTPLTTWTEAFDRSPAAVVDLARNNDWETPLLHGWAKAAIVQADPTWAAALVRNDTRESAAGLREAVRWDLHLLLPPADLARIAADFLRREDHLAHRLLAVHPGAWPDELAVAVVETIAHRARTDKHSWQLAELCRAAAMAMPPKYAMHVAGLAEQLDQQPADPSRVRPVAELARTLIFRAEMLDELASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7F4</accession>
    <name>U5W7F4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_34275</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561450.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34275"/>
    <dbReference type="EnsemblBacteria" id="AGZ45113">
        <property type="protein sequence ID" value="AGZ45113"/>
        <property type="gene ID" value="AFR_34275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6935"/>
    <dbReference type="eggNOG" id="COG5555">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_496898_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665624" evidence="4">
        <location>
            <begin position="27"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="543" mass="54127" checksum="F764D76DF19A9E57" modified="2014-01-22" version="1">MFIKYAASAALSTVVLTLLGPVAAHAAAGPQTITRDGVSIKYQRVAAAATPKSGSGATRSDFDGDGLDDIAATVRGGVVVRYSSGRFIDYFGNTTGSTALTLGTAFTSGDFDNNGFDDLVVGDPDETDKANGTAAGGLWIVPGSAQGLQVSKIRHLNQSTAGVPDASESGDEFAGALAAGDINGDGRADLAVGLPGEDVGTQASAGGLIMLLSDANGVSTTGAKWLDQNTSLVPDTAQSWDRFGGVLAIGKFNADAYADLAIGSPGENEGATATPSKGSINLILGGASGLNVRGVASVYGDDFTQVAPGVKPIRLGFGGITLADTNNDGRDDLVAGTPEATVDGKANAGMVVTLFGGNNGFFAGESQVSTQNSAGIPGDAEAGDSFGASVTAGDITGDGFGDVVIGVPGEDVVDAPLAPPGNVVDAGVISLLRGSATGPAGGVALELSQGYEYTPGTSERGDRFGDSVRVLDLDGRDGLDAIVSAPGELGVDEGGPAPTDGDTPGAVTEYAGGEPNFMYGVRRTTGTELRLPGFNTNIYGTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7L1</accession>
    <name>U5W7L1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_34610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561515.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34610"/>
    <dbReference type="EnsemblBacteria" id="AGZ45178">
        <property type="protein sequence ID" value="AGZ45178"/>
        <property type="gene ID" value="AFR_34610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7002"/>
    <dbReference type="HOGENOM" id="CLU_1691712_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="17043" checksum="072298104CD9FB04" modified="2014-01-22" version="1">MRGRWPVQLARGAGVVVLAVLVGVGVRQGWFPEWNWIERVSWLAGIVGVVVALLALRKPQQRPARTPRQDRRFLPDPRQELKVFAGTGWSSTLRELYPGTKLVTFGGREFPIWAELGAAGCTSVSPPSRRPRRLRVPVLSSTAGCGPQPSPSPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Y0</accession>
    <name>U5W7Y0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561659.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35335"/>
    <dbReference type="EnsemblBacteria" id="AGZ45323">
        <property type="protein sequence ID" value="AGZ45323"/>
        <property type="gene ID" value="AFR_35335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7150"/>
    <dbReference type="eggNOG" id="COG1061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020861_2_0_11"/>
    <dbReference type="OrthoDB" id="458022at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="24"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="573" mass="62730" checksum="9B772AB310EEDF5A" modified="2014-01-22" version="1">MSPPLPDLETFPPLRAWQRKALVEYLRRRSPDFMAVATPGAGKTTFALRIAAELLADGTVEQVTVVCPTEHLKTQWASAAARVGIQLDPSFRNADVHSSRDFHGAVLTYAQVGMAPAVHRRRTMTRPTMVILDEIHHAGDSRTWGDGVKSAFEEAERRLMLTGTPFRSDDNPIPFVTYERGDNGLQRSRSDSIYGYSDALRDGVVRPVIFLAYSGETRWRTSAGDELAARLGEPMTKDLIAQAWRTALDHRGDWMPQVLRAADARLSRLRSHGMVDAGGLVIASDQQTARAYAKLLGEITGEPAVVVLSDDQGASARIGTFALSDQRWMIAVRMVSEGVDIPRLAVGVYATSASTPLYFAQAIGRFVRARVTGETATVFLPSVPHLLGLASEMEAERDHILGAPKDPDGLDDALLERAQRAEDAEGDLDKRFEALSATAELDQVIYDGTSFGTGARTGTPEEEEYLGLPGLLTPDQVSVLLNKRQTDQLAAQKRQKAAEPVPAQREPVVVLSAGERRNSLRRQLNTLVAAHHHRTNLPHGKIHAELRRLCGGPPSAQATIEQLEERIATIQTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-02-10" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W804</accession>
    <name>U5W804_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_29195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29195"/>
    <dbReference type="EnsemblBacteria" id="AGZ44100">
        <property type="protein sequence ID" value="AGZ44100"/>
        <property type="gene ID" value="AFR_29195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5915"/>
    <dbReference type="eggNOG" id="COG2730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044209_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666183" evidence="3">
        <location>
            <begin position="33"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="4">
        <location>
            <begin position="352"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="476" mass="50745" checksum="6302724BAEE10C3E" modified="2014-01-22" version="1">MTGFRAAWVIRAVVALAAIPLALVVAAPSASAATNQFRGVNWARAGDNFTGEPLVLYGLSGSDSYTTVRAKADAVLAGFERNLGANTVRLPINTASVGTAWWSSYTGVIDSATARGMKVILSFWDDGRAASGGRVTDTAAFNTMWNTVVAKYGANSLVYFEPMNEPAGYSAADWKNVAAAWLAARPSVPRNRVFISGSGLNQDIRTMCADSRFDGTYLSLHHYTFFSGAKTYDGWVNFLREALGTCADRTVIDEFGAPMDTGLNYDDAGSTDNFVRYFRATTQVLRELGMGSVYWPGLGGKITAGQNDDWYAMQKLQGSGTNLTLTTPNASGAGRLRYSWGLTGTDPVPGNRLRNSGSGRCLDVPGAVTVNNTQVAIYDCGTGTNQQWTGTSARELRVYGTKCLDARGRGTANGTAVVIYDCTGGTNQQWNVNANGTITGVASGRCLDVTQNGTANGSRMQLWDCTGEANQSWSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W895</accession>
    <name>U5W895_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560532.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29670"/>
    <dbReference type="EnsemblBacteria" id="AGZ44195">
        <property type="protein sequence ID" value="AGZ44195"/>
        <property type="gene ID" value="AFR_29670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6014"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119083_1_0_11"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="16634" checksum="751767FDF985BD52" modified="2014-01-22" version="1">MRKVIRRSVRGLLTDDTGRLLLIRRTRPGLAPYWTVPGGGVEPGDATLQDTLARELREELGAVADDLEQVFLHSSSTPEGLAVQHFFWCRLVSLDEEARTGEEFTDASRGGYDLERISLDEVERVDLKPKELKDFVVRNAEALLSVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8C3</accession>
    <name>U5W8C3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35690"/>
    <dbReference type="EnsemblBacteria" id="AGZ45394">
        <property type="protein sequence ID" value="AGZ45394"/>
        <property type="gene ID" value="AFR_35690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7224"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3467">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055292_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024747">
        <property type="entry name" value="Pyridox_Oxase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12900">
        <property type="entry name" value="Pyridox_ox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="229"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="412" mass="45334" checksum="0480AF9D3B5B5582" modified="2014-01-22" version="1">MTDLYQPTDRTTATRNRGRMHYERDAAHAILDEAYDCSVAFVIDGEPRVLPTLHVRVDDTLYLHASTGGRLGLTARSDGVRVAVSVTLLDGIVYARSQFDHSANYRSLVAHGLARPVTADHEKRAAMTALVERVGVGRAADSRPPTTRELATTVVLALSLTEVSVRVRAGGVVDDPEDKTLPHWAGVLPIRRVAGPPETDAGVTAPPPAYLPGSTSPWHTAAPMRGRLVALEPLAPGHADGLLAAIGDDEVWQFLPSLCPQTTEEMTQHIAVASRQQWQGERVSWAQCDPETGEVMGMTSYHDIDPVTRAVGIGHTILGRRWWRTGVNTEAKLLLLERAFDVLGAERVFWYTDIRNERSQRAIARLGATRDGLIRRHRLRPDGTWRDTVLFAMTVDEWPAAAERLRARLAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8J0</accession>
    <name>U5W8J0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561859.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36335"/>
    <dbReference type="EnsemblBacteria" id="AGZ45523">
        <property type="protein sequence ID" value="AGZ45523"/>
        <property type="gene ID" value="AFR_36335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7353"/>
    <dbReference type="HOGENOM" id="CLU_1292151_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="23006" checksum="E69729643C460DE7" modified="2014-01-22" version="1">MTETPVAAISVPYPEWDPPPRKPGRFASWLALVVVLAGAGVAYQQYQSIFGARYAERADFCPAVDVGPLSAGLATPIGPATKVDDGVPPVGDQQYCRITAAESDGTPRVAAVITFTHYPNGAVADVQYAMRRDDVARTVTDRPNRRDLTDSSFGYHDPATNPPRFQVTVADSNLLITMEIAVPAGNPNWQSRFPTAAYSTLNEVIRATLKRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8K9</accession>
    <name>U5W8K9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acetoin utilization protein AcuC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_35430</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Ketone degradation; acetoin degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561678.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35430"/>
    <dbReference type="EnsemblBacteria" id="AGZ45342">
        <property type="protein sequence ID" value="AGZ45342"/>
        <property type="gene ID" value="AFR_35430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7170"/>
    <dbReference type="eggNOG" id="COG0123">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_0_11"/>
    <dbReference type="OrthoDB" id="1443295at2"/>
    <dbReference type="UniPathway" id="UPA00040"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045150">
        <property type="term" value="P:acetoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09994">
        <property type="entry name" value="HDAC_AcuC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003085">
        <property type="entry name" value="AcuC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="His_deacetylse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01272">
        <property type="entry name" value="ACUCPROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0006">Acetoin catabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hist_deacetyl" evidence="5">
        <location>
            <begin position="21"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="394" mass="41771" checksum="08198660A4692F46" modified="2014-01-22" version="1">MAESTAVVWDEALLAYDMGDHPMDPVRIELTMALARELGVLDRAGVQLLVPEPADDAALIRVHRPDYLEAVRIAPHDPFFRGWGLNTPDNPVFDGMHEASARICGATLAAAEAVWSGAATRAVNVSGGLHHAMAARAAGFCVYNDPAVAIARLLDLGAQRVAYVDIDVHHGDGVQTAFYDDPRVLTVSLHESPAALFPWASGFSTEVGGPGAEGSAVNVALPPGTGDAAWLRAFHAIVPSVLRAFSPQILVTQCGADAHRLDPLADLRLSVDGQRAAYIALRSLADELCDGRWVATGGGGYALVEVVPRAWTHLLAVATGAPLDPATETPPGWRKLAADRRPGTGIPTTLTDGIEPAVERWEPGTTPDPVDKAIMATRRAVFPLHGLDPHDIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8P6</accession>
    <name>U5W8P6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30255"/>
    <dbReference type="EnsemblBacteria" id="AGZ44311">
        <property type="protein sequence ID" value="AGZ44311"/>
        <property type="gene ID" value="AFR_30255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6126"/>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_359783_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="700" mass="77080" checksum="352DFD5727D13EB5" modified="2014-01-22" version="1">MPDDFVVSEKQDIGQEKIDRQLSGMVGGIGTGNIRNKGQAAIGDGAMAAESIYYTTYNLRQKGKSWFGTVPGDHLGRLLAGYSPGPSDTELAAALKLRHVVYLSARPGWGRYATAQVALARRHSPDRVVMIHVARGERIVDAVDEGSYSEDHGHVLDAKDAESISFMDLLLIDQHARSRKASVIVIGPLADRGHDLGSYLFPLRRPTAAAVFRSQLTYLLHERGNCVGTCADCGLDCVADYVEKCVSGGELSRHLNETLALEEAARIASVIGTAASDADRDQLVAQILNEQLHAQARLVLRASDAERRADGEHWSGTADYRRAFRLAFGALEGAPMASVYSAAARLVRIHREEQPETAPTPDPIEFELDCLLSYEMQVPDLNSLPVGAPRIARLANPGLVPAMLDVAWNERGLGPRLMRWLGELVTEPQLVVRERAAMMAGLLSFSDFTGVMDGLIAPWARSRSMRPRQAAGLSLLSCAHNPALHPALQGRIKQWMDKTKGNAYTRDTVAWAYAYGLGRYLPYDGLAQLRRIGKDLWQRRSFLVAFGVEQAYTRDRAASLLGELLEWTLAEDNGKRLQVHAARAFVRLSGIETLLGSVRWPELLLRRHRGDVDEQQLADLWLTALCLPSTAWSAWKAFTRWLTLAENDPDVADRFLALLRVLVADGGLRTRLAHHRDHVWAEQRPSSALLTAAAAIIGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8S9</accession>
    <name>U5W8S9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endolytic murein transglycosylase</fullName>
            <ecNumber evidence="7">4.2.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Peptidoglycan polymerization terminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mltG</name>
        <name evidence="9" type="ORF">AFR_30700</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transglycosylase MltG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.-" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560735.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30700"/>
    <dbReference type="EnsemblBacteria" id="AGZ44400">
        <property type="protein sequence ID" value="AGZ44400"/>
        <property type="gene ID" value="AFR_30700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6216"/>
    <dbReference type="eggNOG" id="COG1559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025574_4_0_11"/>
    <dbReference type="OrthoDB" id="708179at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008932">
        <property type="term" value="F:lytic endotransglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02065">
        <property type="entry name" value="MltG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003770">
        <property type="entry name" value="MLTG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30518">
        <property type="entry name" value="PTHR30518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02618">
        <property type="entry name" value="YceG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00247">
        <property type="entry name" value="TIGR00247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="395" mass="42672" checksum="DCAB48256E3C712B" modified="2014-01-22" version="1">MIDELDLAFDEQAEKGRPRHRRGSRGGKGKGGKSAVAFLMVFILLGVLGVGGYLGYEKIKGFFTAADYDGSGTDEVLVTIAKNATLTEIGNTLVDSDVVKSTAAFTEAADKNPKGKNIQSGSYKLRKQMSAESAVTLLLDPKSRVVTGVTIPPGKTARQAYEILTKATGVPLKDFQEAAKDPGELGVPDFWYNRRDGKKAATGSIEGFLFPDTYEFPPKSTAAEILTIMVQKFISVTTELKFVDKVQNTLSVSPYEALIVASLSQAESGTTGDLPKIARVAYNRAYKEKMPLQFDVTTNYWLDLQEKDGKHSGKMTTKELEDPKNPYTTELRVGLPLGPINSPGEAALKAAEAPTAGPWLFFVAVDKDGNSAFATTNDEHNANIQKACRNGIPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Z8</accession>
    <name>U5W8Z8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_36180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561828.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36180"/>
    <dbReference type="EnsemblBacteria" id="AGZ45492">
        <property type="protein sequence ID" value="AGZ45492"/>
        <property type="gene ID" value="AFR_36180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7321"/>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525630_0_0_11"/>
    <dbReference type="OrthoDB" id="767622at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="4">
        <location>
            <begin position="462"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="514" mass="58769" checksum="A8D001DB85A12C20" modified="2014-01-22" version="1">MRITGTGHASMRIDTAAGSILCDPWVNPAYFASWFPFPDNSQLDWETLGNVDYLYVSHLHRDHFDAEHLKRFINKKATVLLPEYPTSQLEDELRELGFTSFYKTKSNEVHELDGGLKVMIQALISPTDGPIGDSSLWVEHNGVRVLNQNDARPTDLSTFAELGHVHAHMLQFSGAIWYPMVYELPQSAKTAFGKQKRDRQFDRTWRYIDDLKADHVFPIAGPPCFLDDELWQFNDIFGDEGNIFPDQSVFMTEYAKVGGTNAVVLLPGSASTLTTETIETTHPTDVDEFFANKKQHLEEMRDRKAPIIAAEKASWRHPEIDVLGELKKRIEPLLEESIYLAKGVGGPVRFDLVSYDGESIESIVVDFPGKQVRPYADEKVRYRFKTQRELIEHLIYIDEGDWVNSLFLSCRFSAARIGQYNEFVYAFFKCLSEERLQYAEGWYDEHERSVDAEDIELGGWTVQRRCPHLKADLSRFGIVDGNVLTCQLHGWKFDLPSGRCLTSVGHKIRAEKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W923</accession>
    <name>U5W923_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M23B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36280"/>
    <dbReference type="EnsemblBacteria" id="AGZ45512">
        <property type="protein sequence ID" value="AGZ45512"/>
        <property type="gene ID" value="AFR_36280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7342"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077601_4_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M23" evidence="1">
        <location>
            <begin position="15"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="12544" checksum="A84207AAC9BF4244" modified="2014-01-22" version="1">MVRRFDPPPQPWLAGHRGVDLAAAPGVPVGASGAGTIVFAGTVAGRGVVSVAHPGGLRTTYEPVTAADLQPGDAVAAGDALGTLEAAHPGCPAAACLHWGLRRGTEYLDPLTLLGLGRVRLRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9A2</accession>
    <name>U5W9A2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560873.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31400"/>
    <dbReference type="EnsemblBacteria" id="AGZ44540">
        <property type="protein sequence ID" value="AGZ44540"/>
        <property type="gene ID" value="AFR_31400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6355"/>
    <dbReference type="eggNOG" id="ENOG5032Y0K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176227_1_0_11"/>
    <dbReference type="OrthoDB" id="2037313at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021425">
        <property type="entry name" value="DUF3072"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11272">
        <property type="entry name" value="DUF3072"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="69" mass="7525" checksum="7EF33ACD8A8353DA" modified="2014-01-22" version="1">MATNEQEPASNTIKDPADWTTGDEPATGAQQSYLHTLASEAHEEVPEELSKAEASQRIDELQEKTGRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9X4</accession>
    <name>U5W9X4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_38745</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="7">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38745"/>
    <dbReference type="EnsemblBacteria" id="AGZ46003">
        <property type="protein sequence ID" value="AGZ46003"/>
        <property type="gene ID" value="AFR_38745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7840"/>
    <dbReference type="eggNOG" id="COG1146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_0_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="11857" checksum="FFD93769FFA1F058" modified="2014-01-22" version="1">MTYIIAEPCVDVLDKACIEECPVDCIYEGNRMLYIHPDECVDCGACEPVCPVEAIFYEDDVPEQWKDYTNANYEFFEGLGSPGGASKVGKIEKDTPFVAAQAPRGDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Z7</accession>
    <name>U5W9Z7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_32440</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32440"/>
    <dbReference type="EnsemblBacteria" id="AGZ44746">
        <property type="protein sequence ID" value="AGZ44746"/>
        <property type="gene ID" value="AFR_32440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6565"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_470639_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="42"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="303"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="411"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="413"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="446"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="590" mass="63215" checksum="C3B4A770B2C1168E" modified="2014-01-22" version="1">MAGIGRKDTTTVDQDPTGSGARPDSDGASTLDLSGRCVGSSYILIRPIGQGATGTVWRGVDRASGEPVAVKLLHESLLRQPKLVTRFVQERTILLMLRHRNVVRVRDLFSVGESLGLVMDLVPGGSLRDHLREHHTLAPGEAARLAAEVAAALAEAHDLGIVHRDLKPDNILLHREDGRLEIRLTDFGIARVLNTPRLTTTHAVVGTPHYMAPEAFQSSATSPAADVYALGVLLYEMVCGRPPYDSDTVHDLMRLHLEGRPERLDGIPDALWEIINSCLEQKPRLRPTTGELIADLSDVARATGDAPALPKPDKSPREPAPVSPPPVSLSPPPEPLPARPVHPSLAGMRKLPPPQPRNQAASWRWGRPWAMTALVVAAVVASGVTTTAWHLGRADGDPGRAAEVVQPTLRPTLPPAASRSPSPRATTKTHTARRPPIAAQALTPVRPQKTPRTTKATTPAPRTPVREAKKYGPDNCNRGFTVANFSTPVVFSPCHSLGAKVQFHGLLAVPSTGDGRIVLSVRDVGSGRTVGSPKVCGGLRFSKDSATHDCGTGTVAPPRGHRYQVILEWLFQGAGRQPAGGIARGNEFDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA28</accession>
    <name>U5WA28_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anti-sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38115"/>
    <dbReference type="EnsemblBacteria" id="AGZ45877">
        <property type="protein sequence ID" value="AGZ45877"/>
        <property type="gene ID" value="AFR_38115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7711"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155928_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024020">
        <property type="entry name" value="Anit_sigma_mycothiol_RsrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014295">
        <property type="entry name" value="Anti-sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027383">
        <property type="entry name" value="Znf_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13490">
        <property type="entry name" value="zf-HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02949">
        <property type="entry name" value="anti_SigH_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03988">
        <property type="entry name" value="antisig_RsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-HC2" evidence="1">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="95" mass="11104" checksum="77C3B76A75D9F5D9" modified="2014-01-22" version="1">MSDVNDDDHKTDCSVVISEVYLYLDLECSDDRRQLIQHHLDDCSDCLREYGIEHEVKALVARCCGDETAPQELRDRLRVKLSEYVVEVETREYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA82</accession>
    <name>U5WA82_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_39170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39170"/>
    <dbReference type="EnsemblBacteria" id="AGZ46088">
        <property type="protein sequence ID" value="AGZ46088"/>
        <property type="gene ID" value="AFR_39170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7926"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003050_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665709" evidence="3">
        <location>
            <begin position="37"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="domain" description="LamGL" evidence="4">
        <location>
            <begin position="790"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="domain" description="LamGL" evidence="4">
        <location>
            <begin position="1049"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1222" mass="128881" checksum="F467DCDE3B4E40F2" modified="2014-01-22" version="1">MNAARNGSILYRFAALAASSVILVPAVFAVAGPAAAAPATPVFDKPAKPKERATEAQAVAAAVATDSRVEVTGARSETTQVFAEPDGHLTLEATAVPQRVRRESGEWAPIDLNLREAPDGTLRPGASVADVRFSPGGTKPLVTLVSEGKKLSIGWPRPLPEPTVAGTAATYAEVFPDVDLVVHATETGFTHVLVVKTPEAAADPAIQQIRYDLGGDATFVATRGGGLQAVAGGRTLASATEAVMWDSARPTAARRALSAEPAAGSTTGAPADTATIAPVAATVEGGDLVLRPDAELLAAPASAFPLYIDPPWSTGKKRWAYATNNNGNNTDVSKARVGKDPEGSRTYRSFFEFSTSFLKSKHIESAYVQMELDHSASCLETWTHMYSTGSIASTPRTKWSPKLSTWLAATASRAPEGDGCDGKGDQTVNFPADVTKAPQVTNKIQAIAKAGSTTVTMGFCACNADGDYESSADRWKKFFPNKAKLIVDYDSKPGKPFNLKISGVTCPSSGSVSIGTAEPTLTATYPDADEDKNQSLTASYEWVEVPSDGHVLSTTPRRTTPAPKSVPAGDAVSSNKLTGVLKDHRYAIRTFATDPKPYQVAGDWSAWCIFRVDTTVPKPPELTVKTSPTLPGTAATVQLSSPNKDVTKFAYGWDDTPLRTVAATGTTTKTATVSLTAPRYGGLTFFAYAIDITQNNGNNAQVEFTVGRPTEAFARWKLETYPLVETPAKAMADANPAAAGDTPLVPDSTAPDVTWAADAHLIGGSAASFNATANSPFGALKSTAALDTSKSFSVAAWLRLGDVDDYRTAVSKDGAAMSVFRLQYRVDRNAWCFSVRAKDEPGSELTGACSVAPAVRGKWTHVAGVYDDAELKLRLYVDGVLVSDFTPPATWLATWAGGWNAKGAVVVGRALDTKYTGPVDWFHGEIADVQLFDRALVPADLLGQRADDLNSNLVDEPGIMRPIQVGQWDFDAAGWCYQAGVPDTCTAPDASAFDRPLTLTPGIETGEGVRDAGLVLDATHWASDPDPLAGTPTQEYGFSRDLPVLRTDQAFTVAAWVRLDDSLGTETVVSQDTAGAGFSGFDVRYESTGSKYVFAMRNTATATTQASSVSVAVEDPTVWHHLVAVFDPGLRELHLYVDGGGQPDLDDPAANRHAVAAANTGLVPWQATGSLVVGRSDQPAGFTHWLSGSVDTVTAWQGELTAAAIQTLYDSEAPQADPASAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAH7</accession>
    <name>U5WAH7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acyl-CoA carboxylase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39325"/>
    <dbReference type="EnsemblBacteria" id="AGZ46119">
        <property type="protein sequence ID" value="AGZ46119"/>
        <property type="gene ID" value="AFR_39325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7956"/>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_6_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000438">
        <property type="entry name" value="Acetyl_CoA_COase_Trfase_b_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01070">
        <property type="entry name" value="ACCCTRFRASEB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="2">
        <location>
            <begin position="276"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="524" mass="56584" checksum="62E973F2CCB992A2" modified="2014-01-22" version="1">MTAQQDDFQTTAGKLADLERRIDEAVHAGSARAVEKQHARGKKTARERIELLLDEGSFVELDGLARHRSTAFGLEKNRPYGDGVVTGHGTVDGRQVCVFSQDFTVFGGSLGEVFGEKIVKVLDLAMKIGCPIIGINDSGGARIQEGVVALGLYADIFFRNVRASGVIPQISLIMGPCAGGAVYSPAITDFTVMVDQTSHMFITGPDVIRTVTGEDVGMEELGGARTHNSRSGNAHYLASDEDDAVDYVRALLSYLPSNNLDEPTVLEETADLVAGEADLDLDSLIPDSANQPYDIKRVVEAVVEDFLEVQPLYAQNIVVGFGRVEGRPVGVVANQPMHFAGTLDIAASEKAARFVRTCDAFNIPVLTFVDVPGFLPGTGQEWDGIIRRGAKLIYAYAEATVPKVTVITRKAYGGAYDVMGSKHLGADMNFAWPTAQIAVMGAQGAVNILYRSELAEADDPAALRTELIKEYEDTLANPYIAAERGYVDAVIKPSETRAQVTRALRMLRSKRETLPPKKHGNIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAH8</accession>
    <name>U5WAH8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561332.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33685"/>
    <dbReference type="EnsemblBacteria" id="AGZ44995">
        <property type="protein sequence ID" value="AGZ44995"/>
        <property type="gene ID" value="AFR_33685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6818"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108054_1_1_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase_6" evidence="1">
        <location>
            <begin position="7"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="13517" checksum="E0BB6117E31FAA1A" modified="2014-01-22" version="1">MARIRDIVVDCAYAPALARWWVQVLDDYEIAPYGENDPDPDDDPTVLVLPGPGGGPRLWFTQVPEPKTVKDRIHLDLTADDPVAELKRLTGLGARFLWETDGLTVLADPEDNEFCLLKTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAK5</accession>
    <name>U5WAK5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39870"/>
    <dbReference type="EnsemblBacteria" id="AGZ46228">
        <property type="protein sequence ID" value="AGZ46228"/>
        <property type="gene ID" value="AFR_39870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8067"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108054_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase_6" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="13080" checksum="D2CCA15A18C38EE5" modified="2014-01-22" version="1">MVLEAREPLELARFYAALMEWEIVKDEPDFVGVAPMGSHVEYLAVQRSAGYERPTWPDEAGHQQMMMHLDIEVPDLERAVTAAVSVGATEAAHQPQKSVRVMIDPAGHPFCLYVDGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAL2</accession>
    <name>U5WAL2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46238">
        <property type="protein sequence ID" value="AGZ46238"/>
        <property type="gene ID" value="AFR_39920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8077"/>
    <dbReference type="eggNOG" id="ENOG50326B0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195731_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="80" mass="8190" checksum="278C4FAD49A78A16" modified="2014-01-22" version="1">MPVRLAEPSALGLIRPGDHVDLFRVDEDAEAIATAALVLDVTGADDPSLGGLLLALTPAEAQRTVTGAGRGYAVLIRPDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAN7</accession>
    <name>U5WAN7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33885"/>
    <dbReference type="EnsemblBacteria" id="AGZ45035">
        <property type="protein sequence ID" value="AGZ45035"/>
        <property type="gene ID" value="AFR_33885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6859"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073294_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041444">
        <property type="entry name" value="HTH_41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032791">
        <property type="entry name" value="YhfZ_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14502">
        <property type="entry name" value="HTH_41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14503">
        <property type="entry name" value="YhfZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_41" evidence="1">
        <location>
            <begin position="18"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="YhfZ_C" evidence="2">
        <location>
            <begin position="68"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="32824" checksum="A8C68D82166921DA" modified="2014-01-22" version="1">MKPDVRAHVAQVLLAREVGQRIPTVQELQLSTGAGTGTVVKALRNLQDSGAVTLTARGHQGTIVTGRDVGQLWNAAALGNFRLILPPQGPVEQQGILEVVQAALHDVGVSVVAAFRPGARTRLEDVYQERFHATVTSAGALDRHRENLPGLSGLDLGPGTFYSNGSLVVVEHAGRKPQGRLRVGIDRNSDDHQRLSEAEFGDRDPEYVQCPFVLAPAAVLAGDVDVAVWHSMPTVIPPELAGLRLSPLATAPGRAVVGQISPAVIVTRTLDPGVNALLRQVKPADVARTQSRLLKVAATESYAGSLRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAQ5</accession>
    <name>U5WAQ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40220"/>
    <dbReference type="EnsemblBacteria" id="AGZ46298">
        <property type="protein sequence ID" value="AGZ46298"/>
        <property type="gene ID" value="AFR_40220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8140"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="361" mass="38520" checksum="441874F45E3DF0A4" modified="2014-01-22" version="1">MSRYLGARVASRLAADPRVERVIGLDPHEPSPALAGLLTDVEQIRADARASASVLAELGAEVIVHLAVTSAPDPHSGGRAAMKEQNVIGSMQLLAAAQSAGRLRKLVVRSSTAAYGASFRDPAVFTEDTEPRAVPRGSFARDILDIEGYVRGFRRRRPEVDATVLRFAPFISSTAETSLTRYFSQPMIPTVLGRDARLQFVHVDDALEILHRSVVEDHPGTFNVAGAGVLALSQAVRRAGRISVPLPEGTLSSLAAVARNVGIGQIGLDQIDLFVHGRVVDTSRLVEEFGFTPRTTAAAFDDFINGHRNGASLSADRLVAAERAILDGIRRVRAAAAQDASPDASQDASKDASQDEEHVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAZ4</accession>
    <name>U5WAZ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562651.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40325"/>
    <dbReference type="EnsemblBacteria" id="AGZ46319">
        <property type="protein sequence ID" value="AGZ46319"/>
        <property type="gene ID" value="AFR_40325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8161"/>
    <dbReference type="eggNOG" id="COG4122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062591_0_0_11"/>
    <dbReference type="OrthoDB" id="1244860at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28764" checksum="6994237C4C6E8E14" modified="2014-01-22" version="1">MKPVGAVTRGTTNPNRLRRVDNYLAHRCGELLRAADDPLVVDLGYGATPVTAVELRARLAEAVRSDVAVVGLEIDPVRVSAAQPYADPPRLEFRRGGFELAGLHPVVVRAFNVLRQYAEDEVAAAWATMTGTGALLVEGTCDELGRIATWAVVDTGGPQTLTLSARLSDLDHPATFAERLPKALIHHNVPGSPVHDLLATLGAAWDASATPFGSRQRWLGTVAKFREQWPVLDGPARWRRGELTVPWPGQPAGPARDADRPGSRGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB92</accession>
    <name>U5WB92_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34945</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561581.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34945"/>
    <dbReference type="EnsemblBacteria" id="AGZ45245">
        <property type="protein sequence ID" value="AGZ45245"/>
        <property type="gene ID" value="AFR_34945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7071"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050048_0_0_11"/>
    <dbReference type="OrthoDB" id="1809950at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="5"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="189"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="356" mass="38081" checksum="50D5CA68A17EB05D" modified="2014-01-22" version="1">MALWRIRATVDDRPGYLSVLTASLALRSVNILAVQVHTTEGGAVDDFLVDTPDTMTEAELHAAIARGRGREAFVTRAEAQGLADQPTRALALAGRLVHDPDSLGEALVTLLDAAEVRWRPEGTVVLHGFDEERMTLIDPAGGSYEVLRPAPAFTPAEYARAQALVEVSGALVRRTTEQVTLLLPDGSELLIRTAGGDDLEAVRGLHTRSSSQTRRRRYLSGSEVPSDARLRRMLEPATGTTLLAVHADPVSGEEQVVALATLLTEGDLGEVGLLVEDSWQRRGLGTALLRRLITAATRLKASAIVAHTGAENVAMLRTLRRFGVTGRGDRDGDLLSLTLPMTGRQPAGKETPATSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB96</accession>
    <name>U5WB96_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative HTH-type transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_41145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562815.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41145"/>
    <dbReference type="EnsemblBacteria" id="AGZ46483">
        <property type="protein sequence ID" value="AGZ46483"/>
        <property type="gene ID" value="AFR_41145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8328"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_0_11"/>
    <dbReference type="OrthoDB" id="1844486at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17389" checksum="E9D0EF7BACC13D02" modified="2014-01-22" version="1">MAVQDVHLDALDGKMLALLAEEPRIGVLELSRRLGVARGTVQARLDKLTARGAIRGFGPDVSPAVIGYGVMSFVTLEISQRYGHTAVTEHLTDIPEVLEAHTITGSGDLMCRIVARSNADLQRVIDRVLAYEGIERASTIIALAEQIPYRTMPLVKAASQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBU6</accession>
    <name>U5WBU6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transporter inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_35875</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35875"/>
    <dbReference type="EnsemblBacteria" id="AGZ45431">
        <property type="protein sequence ID" value="AGZ45431"/>
        <property type="gene ID" value="AFR_35875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7261"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="206"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="86"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="341" mass="37236" checksum="DB080491AA0CDC99" modified="2014-01-22" version="1">MSSVAGKRGGIRGNERVAGWLFVAPVVLVLGVFMLVPILMALWVSFTDWNGQGSPFRSGVPLAGLDNYTGLFSEDGLARQNFMTSLRNNAYYVLAVVPLQTVLALGLALVVNKRKAFFRTAFYFPSVTSSVAVSVVFLFMFANTGAVNQILSFLGINGPQWFSDPRGVLHLALDGVGLLDSTAPPAALTGHGVLGLTWWDWTSGPSVAMCAIIALVVWTTSGTFMLMFLAALQNLPVQLDEAGLIDGASRWQRLRHITLPQLRPTLFLVLTLGLIGTWQVFDQIYVMSQGDPAKTTLTPAYLSYRTAFRDFDYGSGAAISFVLFLIIVVFTLLQRRALREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC46</accession>
    <name>U5WC46_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561886.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36470"/>
    <dbReference type="EnsemblBacteria" id="AGZ45550">
        <property type="protein sequence ID" value="AGZ45550"/>
        <property type="gene ID" value="AFR_36470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7379"/>
    <dbReference type="HOGENOM" id="CLU_965165_0_0_11"/>
    <dbReference type="OrthoDB" id="2156587at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="29259" checksum="35F221964C5D2F33" modified="2014-01-22" version="1">MPENDLGHGLGALADHGGRTGRLAAAGDIRARADRRRRRRYVATGACAVVLAGAFGVGIAVARPDAGPRPGPPAATGSTAQTTSPPVVPSPPPSTPPSSSPSSSPSSSASPSSAPGTSGTPSGSATTSVFSGKRQIHLLPVGSESTFGVGASERLGLSEAFGDRDLFVLTPLGSDRYWIRTAKIQVGGEPLCVAVQDDGGAPAPVVATACDAADRKQRFLFRRTGTVDGKPKYTIRTSNNIYLVQDPDGRLISGGTGVVAVEIGEGTPDIDTPFLLPDQGRATLPTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC70</accession>
    <name>U5WC70_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rpsR</name>
        <name evidence="6" type="ORF">AFR_42820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007073789.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="SMR" id="U5WC70"/>
    <dbReference type="STRING" id="1246995.AFR_42820"/>
    <dbReference type="EnsemblBacteria" id="AGZ46818">
        <property type="protein sequence ID" value="AGZ46818"/>
        <property type="gene ID" value="AFR_42820"/>
    </dbReference>
    <dbReference type="GeneID" id="5704586"/>
    <dbReference type="GeneID" id="67458323"/>
    <dbReference type="KEGG" id="afs:AFR_42820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8662"/>
    <dbReference type="eggNOG" id="COG0238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148710_1_0_11"/>
    <dbReference type="OrthoDB" id="1940575at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00270">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_S18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018275">
        <property type="entry name" value="Ribosomal_S18_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_S18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="PTHR13479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="SSF46911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00165">
        <property type="entry name" value="S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00057">
        <property type="entry name" value="RIBOSOMAL_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="79" mass="8944" checksum="DB4BD2FCF010AC48" modified="2014-01-22" version="1">MAKAAALRKPKKKVNPLDKDGITYIDYKDTALLRKFISDRGKIRARRVTGVTSQQQRQIARAVKNAREMALLPYTATAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCB7</accession>
    <name>U5WCB7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative LacI-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_36930</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561976.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45640">
        <property type="protein sequence ID" value="AGZ45640"/>
        <property type="gene ID" value="AFR_36930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7472"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="36115" checksum="C6FBC9424958E8D8" modified="2014-01-22" version="1">MKRPTIADIARRAGVSKGAVSYALNGQPGVSEATRQRIVAIAQEIGFNPNSAARALSGASARAVGLTLCRPARILSIEPFFMGLISGFEQELAARSFALTLQVVATPQNEIDVYRRWWGERRVDGVFVTDLRENDIRIPVLNELQLPAVVIGGPGDTGNLAQLWSDDAGAITEAVRYLMALGHRRIARVGGLPDLLHTQTRTRAFTEVCASLGLSEAVTVPSDYTGEEGGRATRRLLINAERPTAIIYDNDVMAVAGLSVAQEMGLSVPGDLSIVAWDDSPLCSLVHPPLTALSRDIAAYGAHAARQLLAAIAGEPVKDVEEGTAHLTPRGSTAPPRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCJ6</accession>
    <name>U5WCJ6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">def</name>
        <name evidence="4" type="ORF">AFR_42190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563023.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42190"/>
    <dbReference type="EnsemblBacteria" id="AGZ46692">
        <property type="protein sequence ID" value="AGZ46692"/>
        <property type="gene ID" value="AFR_42190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8538"/>
    <dbReference type="eggNOG" id="COG0242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_545028_0_0_11"/>
    <dbReference type="OrthoDB" id="1649129at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="17"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="513" mass="58668" checksum="D0E8A007EDDF4D18" modified="2014-01-22" version="1">MTTSPVERAADHFVSALAQWRVERGMTKKQLAARMGFDPSYVSHVEGRRHKPTEDFARRAEAVLAAGGAIWQRFQEYDELRHARGPVLHRDPPVPAQWMPPGTGLIVEQEIAELTYTEGAYRCVIRRALYNAGIEPVTRYLVKIAVDRYPHDPQGSNRHHRQHPLSFEEMDLRAVAGEGEATERMQWRLKLDRDAAKEVWLLFQNEHGQFPLYPGERTTIEYAYSVGEEKWGQWFQRAVRLPTRNLTVRLDFPVEFEPQVWGVETSLAAEVPVRTPLERHSDARRAVFEWSTDAPLLNARYRLEWRFRGQAAPTTPDEGVPKPEHNGHELRASQRMRGIGIVQRGADLLRQRARHFQLPREEPAARDAVAGLRMILDRLEGLHDFSKGVGLAAPQIGLAVAAAVVRPPERGAEPVILLNPRVVDESSETDEQYEGCLSFFDYRGLVVRPLRVDVEHARFDGTRMITSFERAMARLVSHEIDHLEGRLYVDRMVPGANLVPVDEYRQTGRPWSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCY5</accession>
    <name>U5WCY5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563172.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42940"/>
    <dbReference type="EnsemblBacteria" id="AGZ46842">
        <property type="protein sequence ID" value="AGZ46842"/>
        <property type="gene ID" value="AFR_42940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8687"/>
    <dbReference type="eggNOG" id="COG0860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049583_0_0_11"/>
    <dbReference type="OrthoDB" id="1184688at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00646">
        <property type="entry name" value="Ami_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MurNAc-LAA" evidence="1">
        <location>
            <begin position="239"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="383" mass="41528" checksum="21437448434C536F" modified="2014-01-22" version="1">MRSIRRGDTGPAVEEIQSILVGLELLPAPGETFDQATENAVRAFQQSRGIGVDGMVGPETWQALDGARWKLGARTLYHSVPTSLVGEDVRALQERLLEMGFDSGRADGIYGPRTARAVAQFQREVGLTPDGSCGPQTMKGLRRLGRKVVGGRPQWLREAEAFRQSGSSLVGKTIVIDPGHGGAEDLGVVVPDGQLRWTEADLSFDIATRLEGRLLAAGMRVHLTRGPSPAQPMTGQERAALANSLGADLLISLHLDGQESPAADGVASYHYGTGNGLTSTVGERLANLVQREIVVRTGMRDCRIHSKTWELLRLTRMPAVRVDLGYLTSPVDRERLIDPSFREQIVEAILAAVQRMYFPVEADVPTGSIDVRQLRLALASRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDR2</accession>
    <name>U5WDR2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative ATP/GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_39530</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562494.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39530"/>
    <dbReference type="EnsemblBacteria" id="AGZ46160">
        <property type="protein sequence ID" value="AGZ46160"/>
        <property type="gene ID" value="AFR_39530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7997"/>
    <dbReference type="eggNOG" id="COG2229">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077970_1_0_11"/>
    <dbReference type="OrthoDB" id="1438064at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="21264" checksum="A986EA8E4AB0D23A" modified="2014-01-22" version="1">MSRRPAAGRVTSAKIVIAGGFGVGKTTLVGSVSEITPLTTEAIMTSAGVGVDDNRQVPGKTTTTAAMDFGRISIDRDLILYLFGTPGQTRFWFMWDELVRGAIGAVVMVDTRRLADCFAAIDFFEHRRLPYLVAINCFDGMQYHNAQDVRDALAISSDVPVVSCDARNRESTKNVLISLVEYVLTMRRSRAVAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEC3</accession>
    <name>U5WEC3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562717.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40655"/>
    <dbReference type="EnsemblBacteria" id="AGZ46385">
        <property type="protein sequence ID" value="AGZ46385"/>
        <property type="gene ID" value="AFR_40655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8228"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081826_0_0_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="60"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="296" mass="31611" checksum="020480B6EECC2FD9" modified="2014-01-22" version="1">MKGLRWPPPPDGGPRNPGPGPSAPRGGRPTLPQPPTELVATRYGVRLEQLVTGTGDPVTVFAHGLAGDIAGTRPLGSGVLGRRVFFHFRGHGRSDAPPGPWSFTDLADDLRGIADRSGATRALGISMGAGALCRLLADTPDRFERIVLYLPAPLDGVRPPVAEARLTRLLTAVESGEAALVAEAVEPELPPSVRNTPTGWSYLRQRVEQLLTDGLAPELDTLWRSPAVPDDAVLGAYPGQALVIGCVGDDVHPVPVAERLSRLLPGAELVVYDRPAVLWTKRKELRERVSTFLNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEH1</accession>
    <name>U5WEH1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40930"/>
    <dbReference type="EnsemblBacteria" id="AGZ46440">
        <property type="protein sequence ID" value="AGZ46440"/>
        <property type="gene ID" value="AFR_40930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8286"/>
    <dbReference type="HOGENOM" id="CLU_2420370_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666524" evidence="2">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="91" mass="8727" checksum="69CFE970A02598C5" modified="2014-01-22" version="1">MRLRRHAALAIAALTLATVAACGDDPEEKSTPAPAPSVVTSADAAPAPATSATTSAAPSTPPTEGTSSAKPPKKGSGAAQGPAGGREVAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEN5</accession>
    <name>U5WEN5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BadF/BadG/BcrA/BcrD type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562837.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41255"/>
    <dbReference type="EnsemblBacteria" id="AGZ46505">
        <property type="protein sequence ID" value="AGZ46505"/>
        <property type="gene ID" value="AFR_41255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8349"/>
    <dbReference type="eggNOG" id="COG2971">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016274_1_2_11"/>
    <dbReference type="OrthoDB" id="1589192at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BcrAD_BadFG" evidence="1">
        <location>
            <begin position="11"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="342" mass="34833" checksum="337036A586564F10" modified="2014-01-22" version="1">MGAMRALFLAVDGGNSKTDVILGTTEGEVLGFVRGPTSSPHNIGLPGTISVLHTLITAVRADAGLPASTGIDVVAAYLAGADLPVEVQRLHQAIDGQSWAARTIVDNDCFALLRAGTSLPDAVAVVCGAGNNCAGRTADGRTARFVALGPISGDWGGGHDLAEHTLRLAARGEDGRGDPTALSAAVAGHFGCPTVEDVSIALHLGDLDLVRVRELTPLLFEVAATGDPVASGLISRQVDEILGQHRVAATRLDLLDSRHALVLGGGVLRARHPQLHDQIVAGARAQAPLVEISVLDTPPAVGAALLALDALGLTEGAERHLRAALAAAEPAPAIPLTVTTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFC0</accession>
    <name>U5WFC0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative CDP-alcohol phosphatidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563066.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42405"/>
    <dbReference type="EnsemblBacteria" id="AGZ46735">
        <property type="protein sequence ID" value="AGZ46735"/>
        <property type="gene ID" value="AFR_42405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8582"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154461_0_0_11"/>
    <dbReference type="OrthoDB" id="1380643at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="25337" checksum="F7539BA184C22DB5" modified="2014-01-22" version="1">MPTPSPTADPTVEKPPTPDDYYRVNRGGGLFSEALSQRIGARIAVFAHKHKLAPTVLTVANLGLSCLVSFVVVASAGPVANDRVWAWPIGLLALVGWQLAYALDCSDGQLARVTGQTSTAGGRVDVLCDVAGQIALVAALSAVAEAQVPETPAWLVAAFAGTWMVNLVTSVMQGGDQAASMVTSRSLPVRVAKLVRDYGAVIAVAGVVLTVAPQWTVWLVSAFTLINGAFLAASIAFSGRAALKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNH4</accession>
    <name>U5VNH4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-Ig-H3/fasciclin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01145"/>
    <dbReference type="EnsemblBacteria" id="AGZ38518">
        <property type="protein sequence ID" value="AGZ38518"/>
        <property type="gene ID" value="AFR_01145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.228"/>
    <dbReference type="eggNOG" id="COG2335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1270064_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="SSF82153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665556" evidence="1">
        <location>
            <begin position="26"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="2">
        <location>
            <begin position="42"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="22422" checksum="58266316E52143DC" modified="2014-01-22" version="1">MATRIAAGATATVLASVALGAPAQAHGKTKPLGTKSLAAVLTADKSGFDRNSRDFDVLTAAVLAVLDAKPNSPVKVLTDGTVALTAFVPNDGAFRQLAKEVTKKRSLPTEQQAFTTVAGLGIDTVEAVLLYHVVPGATVDKKAALKADGAKLTTAAGSKITVDVSRCWYRSPRVTLIDADRNDRNAQVVVFDINKGNKQIAHAVDRVLRPVDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP17</accession>
    <name>U5VP17_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative magnesium/manganese-dependent protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01225"/>
    <dbReference type="EnsemblBacteria" id="AGZ38534">
        <property type="protein sequence ID" value="AGZ38534"/>
        <property type="gene ID" value="AFR_01225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.244"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066586_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039248">
        <property type="entry name" value="Ptase_RsbX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35801">
        <property type="entry name" value="PTHR35801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="160"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="35070" checksum="2067A1FECAC343A6" modified="2014-01-22" version="1">MASLPEGLLDAGVWFRVEAAGTAAAVRRAAERLATELGLPANRIADLSIVAAETAGNLVKHADEGMLLVRPVRVPDRAGVEIVALDRGPGMADIARSLGDGHSTAGTLGIGLGAIVRQASWSDLHSVPGRGTVMSIQVWADEAPPPAWAAGLTRPLTGETVTGDGFAIREADGRRQVLVCDGLGHGHLAHAATQEAVRVFDSTAAAPPAVVVEALHRAIGHTRGAALAVAELDDRAGLVRYAGLGNIAGIVVGADGTRRGMVSLPGIAGHQRRQIREYDYPLLPGAVVIMHTDGIVDRWNATDYPGLITHSPQVIAATVLRDAGTRRDDAGVLVARTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPE0</accession>
    <name>U5VPE0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH:quinone reductase-related Zn-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357781.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02745"/>
    <dbReference type="EnsemblBacteria" id="AGZ38838">
        <property type="protein sequence ID" value="AGZ38838"/>
        <property type="gene ID" value="AFR_02745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.552"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_11"/>
    <dbReference type="OrthoDB" id="1752728at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="10"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="31583" checksum="6587FF45123AC0FE" modified="2014-01-22" version="1">MKALRYHEFGDTSVLRLEDVEQPVPAAGQVLVKVAATSFNPVDNGLRGGFLQPVMPIELPHIPGIDVAGTVVEGPGFRPGDAVVGFLPMTADGAAAEFAVAPAEILTAAPATIPLADAAAVPAVALTAWQGLFEHAQLQAGQRVLINGAGGGVGGYAVQFAKQAGATVIATASPRSTEAVRAAGADQIIDYTATTVADAVDVPVDVVLNLAGVDEKAMAALVALVKPGGVVVTTASPAPEDTERKVRTIQMYVRSDAAQLAEIVRRIDAGEITVDVSGRYPIAETKQVQELGAAGKLRGKVILTTGVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPQ6</accession>
    <name>U5VPQ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative LacI-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_03270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03270"/>
    <dbReference type="EnsemblBacteria" id="AGZ38943">
        <property type="protein sequence ID" value="AGZ38943"/>
        <property type="gene ID" value="AFR_03270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.658"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="344" mass="36803" checksum="BA7087CD1E27A57B" modified="2014-01-22" version="1">MVNGRTPSVKDVARSAGVSLGTVSNVMNRPEVVSPSTRERVERAMAELGFVRNESARQLRAGTSRSLAYVMLDGSNPFFHDVAQGIELAAEDADLSLFICNSNSRAEREEVHLDRLMQQRVQGILITPVNPEAAHLGEISRRGIPVVIVDRVGVGGAFCSVAVDDVLGGRIAVEHLAEQGHTRVAVVGGPESIGQVRERVEGARQIWAELGLPPEDLTYLPTAALTVAEGRSAGERLAGIPARRRPTAAFCANDLLALGLLQQSIGAGLRVPEELAIVGFDDIEFAAAAAVPLTSVRQPRQELGRAAAQLVLDEATNPDHEHQQLSFVPELVARASTVVRSRAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQE1</accession>
    <name>U5VQE1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04295"/>
    <dbReference type="EnsemblBacteria" id="AGZ39148">
        <property type="protein sequence ID" value="AGZ39148"/>
        <property type="gene ID" value="AFR_04295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04295"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_2_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="233" mass="25688" checksum="ACDE8EFF78DA7A04" modified="2014-01-22" version="1">MMRRAEVREKLRELIDSCEPGDALPSERDLSDDFGASRPTIRAAIEELAQEGLLVRRHGRGTFTNHRKISQEVPAAAALPPAEGEWDSEVLEFGVVSAGARLSMRLQVSPGDDVIRALRRRVVDAAPIALEEIRLPLSIAPGLTADDFAAGSLYRHLRERHGITPATAVQTTEPTVTDAIESRLLGVPRYSPALLFERTTSDTHGRVIEFARSVYRGDRYRITNHLTFGPESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQH5</accession>
    <name>U5VQH5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpmC</name>
        <name evidence="6" type="ORF">AFR_04545</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358182.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04545"/>
    <dbReference type="EnsemblBacteria" id="AGZ39198">
        <property type="protein sequence ID" value="AGZ39198"/>
        <property type="gene ID" value="AFR_04545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.918"/>
    <dbReference type="eggNOG" id="COG0255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158491_3_3_11"/>
    <dbReference type="OrthoDB" id="2063644at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="78" mass="8762" checksum="D7D072C856F619CA" modified="2014-01-22" version="1">MAAGVKASELRELPEEELVSRLRESKAELFNLRVQGATGQLDNHRRLQVVRKDIAKIYTIMRERELGLSAAPTEVTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQL4</accession>
    <name>U5VQL4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Phosphoglucosamine mutase</fullName>
            <ecNumber evidence="6 8">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">glmM</name>
        <name evidence="13" type="ORF">AFR_04795</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="6">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="6 8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358276.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04795"/>
    <dbReference type="EnsemblBacteria" id="AGZ39248">
        <property type="protein sequence ID" value="AGZ39248"/>
        <property type="gene ID" value="AFR_04795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.968"/>
    <dbReference type="eggNOG" id="COG1109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_0_11"/>
    <dbReference type="OrthoDB" id="1265792at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05802">
        <property type="entry name" value="GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_B">
        <property type="entry name" value="GlmM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006352">
        <property type="entry name" value="GlmM_bact"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01455">
        <property type="entry name" value="glmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="10">
        <location>
            <begin position="3"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="11">
        <location>
            <begin position="161"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="12">
        <location>
            <begin position="260"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="9">
        <location>
            <begin position="376"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="6">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="6">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="6">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ39248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="450" mass="46068" checksum="94C25D4213CD7B99" modified="2014-01-22" version="1">MGRLFGTDGIRGLANGDVLTPELALSVAVAAARVLIESDASHQPLAIVGRDPRASGEMLEAAVVAGLTSAGANVVRVGVLPTPAVAYLVGQAGADLGVMLSASHNPMPDNGIKLFAPGGQKLPDELEEKIEKAIADGHGLVGRPTGAGVGRVHDLLDGAEHYIKHLLEATPHPLNGVKVVVDCANGAASEVGPTAYEEAGAEVIKIHAEPDGLNINDNCGSTHLEAVREAVLEHGADLGLAHDGDADRCLAVTAAGDVVDGDEIMAILALAMRDAGTLTDDTLVATVMSNLGLRLAMKQAGIRLLETKVGDRYVLEELASGGFALGGEQSGHIVMPAYATTGDGVLTGLHLMATIASSGKSLADLASVLHKLPQVLINVPVKDRQAGAAAPTVQAAVALAENELGETGRVLLRPSGTEHLVRVMVEAATEDQARTIAERVADEVRTASPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQS2</accession>
    <name>U5VQS2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04290"/>
    <dbReference type="EnsemblBacteria" id="AGZ39147">
        <property type="protein sequence ID" value="AGZ39147"/>
        <property type="gene ID" value="AFR_04290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.866"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033532_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="469" mass="47450" checksum="5DB761B44E0CDC51" modified="2014-01-22" version="1">MRYLTVWRIPYAPTLLLAGVVARLGIGMTPLALLLLVEEATGRYAAGGLAVGCYAVAGAIANPVAARVADRVGAAQVLRATAAAHAAILMLLALVATAGLPLVLVVSALAGATYPPLTGAIRRVWTGLTGREPHARRQAALAAETSLFELVYVIGPLLVAALATFTDGYGPVLFVAAAVTFAGGLLVSRVPAMQERHPPGRHSMRRVPGFGTLLVCVALLGGAFGAVTVGVPAFAAGHGGGAGLGGLLLGVWGVGSTIGGLWFGTRRPRRDPCGQYAVLLAAVGFGFLLLIGASGPWSLSLVLVLGGVTMAPALTVENNLVGRIAPGAALNEAYTRVLATAIVSSAAGSALAGVIVDRPGGVRWAFALAGGLAMAAALVGARPAGALARADRLLTARQRSAADVARMLPSLRPQSARSAGLRPERQVVRDAVPVAAVDGSGELDDPTVGVARRAFEEQGGGVARHPQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQW9</accession>
    <name>U5VQW9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplE</name>
        <name evidence="9" type="ORF">AFR_04565</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358189.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04565"/>
    <dbReference type="EnsemblBacteria" id="AGZ39202">
        <property type="protein sequence ID" value="AGZ39202"/>
        <property type="gene ID" value="AFR_04565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.922"/>
    <dbReference type="eggNOG" id="COG0094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_2_1_11"/>
    <dbReference type="OrthoDB" id="1456375at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_B">
        <property type="entry name" value="Ribosomal_L5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_L5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L5" evidence="7">
        <location>
            <begin position="33"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L5_C" evidence="8">
        <location>
            <begin position="93"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="21306" checksum="6C9A995A1FF5F3EE" modified="2014-01-22" version="1">MTAATETKTLPRLKQKYRSEVISALQEQYKYGNPMQVPGLVKVVVNMGVGEAARDAKLIDGAVRDLTTITGQKPLVRRATKSIAQFKLREGMPIGAKVTLRNDRMWEFLDRLLSIALPRIRDFRGLDGRKLDGHGNYTFGLTEQSVFHEIDQDRIDRTRGMDITVVTSAATDEEGRQLLKLLGFPFKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRR5</accession>
    <name>U5VRR5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Adenylosuccinate synthetase</fullName>
            <shortName evidence="8">AMPSase</shortName>
            <shortName evidence="8">AdSS</shortName>
            <ecNumber evidence="8 10">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purA</name>
        <name evidence="11" type="ORF">AFR_00680</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="8 10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357369.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00680"/>
    <dbReference type="EnsemblBacteria" id="AGZ38425">
        <property type="protein sequence ID" value="AGZ38425"/>
        <property type="gene ID" value="AFR_00680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.136"/>
    <dbReference type="eggNOG" id="COG0104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_11"/>
    <dbReference type="OrthoDB" id="232152at2"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="IMP binding" evidence="8">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="8">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="298"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="412"/>
            <end position="414"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ38425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="430" mass="46742" checksum="E4EB8F6B8889CFC1" modified="2014-01-22" version="1">MPVIVLVGAQWGDEGKGKATDLLGDRIDYVVKFNGGNNAGHTVVIKGEKYALHLLPSGILTPGVTPVIGNGVVVDLAVLFQEIEGLEARGIDTSRLRISANAHVIAGYNRTLDKVSERYLGARRIGTTGRGIGPTYADKMNRLGVRIQDLFDESILRQKVEAALSFKNQVLSKIYNRSAIKPDEVIADLLSYTERLRPMVADTALELSQALDNGKVVLCEAGQATLLDVDHGTYPFVTSSNATAGGACTGSGIPPTRVDRVVAVLKAFTSRVGEGPFPTELHDEVGDYLREVGHEYGTTTGRPRRIGWLDLVMGRYAQRINGVTDFALTKLDNYDGLDEIPVCVAYDVNGVRHDEMPYSQSDFHHATPIYETLPGWKKDISGARSFAELPDNAQRFVEFVEARIGARISVVGVGPGREEVIERHSVLSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRS2</accession>
    <name>U5VRS2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00360"/>
    <dbReference type="EnsemblBacteria" id="AGZ38361">
        <property type="protein sequence ID" value="AGZ38361"/>
        <property type="gene ID" value="AFR_00360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.74"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033540_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="392" mass="42096" checksum="F1C498DA106878B8" modified="2014-01-22" version="1">MGGLIRARRLLRGWSVRYAASRAGVSHATWSRIERGLQAADNRFMVADIAGALECSPADLVSTEVPAPDRVTKAARARVLGLRRALIDIDLSEAPAGAALPMVELSRGVALVDALHQACDYAGAARLLPGLLSDLHTETAGPDRYEALRLLCDATSNASSVLRSLGYPADAFLAAERCRDAADAAGDPVLQGHAAYILARAATACGSYQRGQTIAERAIDDLGRHIARPGGPEALGSLHLVAALASRCRERLDDSREWLDEAAELAQRTGETDTMGMFFGPTNVDIWRMSIEVDDGDPGRVVEIAQRTDPATIPAGVRQVFYYADTARALARIGGRDREAIRFLLTAERIAPQHVHTSAELAETTRALLDRSRRQAGGSELRGLSERMQVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRW3</accession>
    <name>U5VRW3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07120"/>
    <dbReference type="EnsemblBacteria" id="AGZ39713">
        <property type="protein sequence ID" value="AGZ39713"/>
        <property type="gene ID" value="AFR_07120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1453"/>
    <dbReference type="eggNOG" id="ENOG502ZJI8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2735591_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="67" mass="7167" checksum="F1B0932C7DEBACDF" modified="2014-01-22" version="1">MSDDRIDQRAADLLPEERNPGSSDPGAQAAAILADSDEREEDLEAAPDSFLEHRTSGQTVTPGEGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS14</accession>
    <name>U5VS14_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357404.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00860"/>
    <dbReference type="EnsemblBacteria" id="AGZ38461">
        <property type="protein sequence ID" value="AGZ38461"/>
        <property type="gene ID" value="AFR_00860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.172"/>
    <dbReference type="eggNOG" id="COG4319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132094_0_0_11"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13474">
        <property type="entry name" value="SnoaL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="15248" checksum="7DAE006789DD7B8D" modified="2014-01-22" version="1">MDSAKITQLVHHRAAAVSAKDAAAIVASNGPTIVEYNLAPPLRQPSAGRDPVPLEKWLATFEGPMSMQVTDLEVTVDGEVAFTTSLNCLTATPAGSTESFSLWFRATLGLRKTSGEWQVVHSHESVPFDMDGTFQASINLHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS58</accession>
    <name>U5VS58_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_07670</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07670"/>
    <dbReference type="EnsemblBacteria" id="AGZ39823">
        <property type="protein sequence ID" value="AGZ39823"/>
        <property type="gene ID" value="AFR_07670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1564"/>
    <dbReference type="eggNOG" id="COG1530">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003468_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019307">
        <property type="entry name" value="RNA-bd_AU-1/RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004659">
        <property type="entry name" value="RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30001">
        <property type="entry name" value="PTHR30001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10150">
        <property type="entry name" value="RNase_E_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00757">
        <property type="entry name" value="RNaseEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="396"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="91"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="797"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="878"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="898"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="922"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="961" mass="103377" checksum="AF2895AABD7EF6C1" modified="2014-01-22" version="1">MEEPAAPAAVAEDPAAVAESLLSDGEPVAPVKATRARRKKAVPPAAPPEPVAEEPVAEDEPEAVAEEVPPAVVETPLIGVLPLDNDLVEEAPAPRTRSRKAAALPPAVLFMPPEPETEPAPTRRTRRGAAAAPVVTEPVAAEPAVTDLFATEPVEAVSEEPAAEEEPAEGTRRPARSRRRRGAAAEEPVEVAEVAEVVEQPVLEEADETADDIEDEDELDEDGNSRRRRRRGRRGRGRGKGPSDEGDDEPEASAEGSADAESEDEEDAEDGDGVTRRRRRRRRKGSGDTEAGGIEDGVHTVVRVREPRTRERSDEVQGVSGSTRLEAKRQRRRDGREQRRTRPPILSESEFLARREAVDRVMVVRQKPDRTQIAVLEDGVLVEHYVSRTTSGTMVGNVYLGKVQNVLPSMEAAFVDVGRGRNAVLYAGEVNWDATGLEGRARSIEQALRSGDSVLVQVTKDPIGHKGARLSSHIALSGRHLVYVPNGNASGISRKLPDNERKRLRDVLKKLVPDGAGVIVRTAAEGASEDELARDVKRLQAQWEDIQAKAAEGHAPAALSEEPDLVIRVVRDLFNEDFREVVIQGEQAFGEVESYLESVSPELLDRLHRHVGAADIFAEKRIDEQILKGLDRKVFLPSGGHLVIDRTEAMTVVDVNTGKYTGAGGNLEETVTRNNLEAAEEIVRQLRLRDIGGIVVIDFIDMVLESNRELVLRRLTECLGRDRTKHQVTEITSLGLVQMTRKRIGAGLLEAFTETCDHCKGRGLIIHTEPVPEKRVGGNGAGGGAAVTQVKAVAAAARTEAPVQQPATTKSRRRRGGGGAEQPAESEVAVAEVTEAPEVVEAPRERVTEDPVAETAGLPPQGSGIVAAAGSGIVARTAPTATSSTSDDDYDISGYDLSRYESNGTNGTGPSAEPLQLTGSDDPDAAEDDEDGEGDDDEPVGAGAGGRRRSRRGGTRRRTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS78</accession>
    <name>U5VS78_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359240.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07100"/>
    <dbReference type="EnsemblBacteria" id="AGZ39709">
        <property type="protein sequence ID" value="AGZ39709"/>
        <property type="gene ID" value="AFR_07100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1449"/>
    <dbReference type="eggNOG" id="ENOG5032Y7X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179200_1_0_11"/>
    <dbReference type="OrthoDB" id="2009249at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046156">
        <property type="entry name" value="DUF6158"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19655">
        <property type="entry name" value="DUF6158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="85" mass="9451" checksum="154B728F5EA5967D" modified="2014-01-22" version="1">MTDARLDSGDDELVVSETGIDPAGLADDDLLRELASLHRTRLDTLRHGPDAALSNHLRRTAELETEYLARHPGREVDASRLTQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSF1</accession>
    <name>U5VSF1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07265"/>
    <dbReference type="EnsemblBacteria" id="AGZ39742">
        <property type="protein sequence ID" value="AGZ39742"/>
        <property type="gene ID" value="AFR_07265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1483"/>
    <dbReference type="eggNOG" id="ENOG502ZTAF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191949_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="85" mass="9760" checksum="1FD72822987ABA3E" modified="2014-01-22" version="1">MLVVMDDDRDLEFEVGELLYDLCVRLGFCLPPEANRRLVEAPPAGVDAFTDAVFEAEGMGDMSYTDLRRQVRAVVDQHMSRWPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSS3</accession>
    <name>U5VSS3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NIPSNAP family containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359445.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08125"/>
    <dbReference type="EnsemblBacteria" id="AGZ39914">
        <property type="protein sequence ID" value="AGZ39914"/>
        <property type="gene ID" value="AFR_08125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1658"/>
    <dbReference type="eggNOG" id="COG1359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100911_0_0_11"/>
    <dbReference type="OrthoDB" id="1925073at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIPSNAP" evidence="1">
        <location>
            <begin position="3"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="21998" checksum="6A8EAA6C178440B9" modified="2014-01-22" version="1">MIVELRQYTLHPGRRDELIELFDREFVETQEETGMTVLGQFRDLNNPDRFVWLRAFPDMKTRASALEAFYGGPVWAEHSDQANATMITFDDVLLLRPAGPGFVLPATRPTEPASSRFLATLHFADQPFDPEPAPATFTTAYEQNTFPALPVRENEHVLVTFTRFDDQAALDEHLADAPAETHLVLAPTDRSRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT78</accession>
    <name>U5VT78_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DeoR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_02860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357804.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02860"/>
    <dbReference type="EnsemblBacteria" id="AGZ38861">
        <property type="protein sequence ID" value="AGZ38861"/>
        <property type="gene ID" value="AFR_02860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.575"/>
    <dbReference type="eggNOG" id="COG1349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060699_1_4_11"/>
    <dbReference type="OrthoDB" id="1629121at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014036">
        <property type="entry name" value="DeoR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00455">
        <property type="entry name" value="DeoRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08220">
        <property type="entry name" value="HTH_DeoR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00037">
        <property type="entry name" value="HTHLACR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00420">
        <property type="entry name" value="HTH_DEOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="3">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="27361" checksum="9EB3DCCA61368E3B" modified="2014-01-22" version="1">MNADERQHQIVALARRQGEVDVAKLSAELTVSAETIRRDLRLLEKHGLLRRTHGGAYPVETAKFETDLAMRTTRGVPEKRRIAAAAVALLGDAETIFIDEGFTPQLIAEALPTDRPLTIVTASLNTAAWLATSSPATVLLLGGRVRGRTLATVDHWASTMLAGFVIDLAFVGANGISREVGLTTPDPAVADVKAQAVRAARRKIFAGIHTKFGVRTFCRFAEISDFESLVTDTGLPASEAHRYSRLGPQVIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTG7</accession>
    <name>U5VTG7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">YD repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_03705</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03705"/>
    <dbReference type="EnsemblBacteria" id="AGZ39030">
        <property type="protein sequence ID" value="AGZ39030"/>
        <property type="gene ID" value="AFR_03705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.748"/>
    <dbReference type="eggNOG" id="COG3209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000662_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022385">
        <property type="entry name" value="Rhs_assc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006530">
        <property type="entry name" value="YD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03696">
        <property type="entry name" value="Rhs_assc_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01643">
        <property type="entry name" value="YD_repeat_2x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666065" evidence="2">
        <location>
            <begin position="18"/>
            <end position="2557"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="3">
        <location>
            <begin position="1744"/>
            <end position="1880"/>
        </location>
    </feature>
    <feature type="domain" description="INTEIN_C_TER" evidence="4">
        <location>
            <begin position="2398"/>
            <end position="2424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2194"/>
            <end position="2274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2198"/>
            <end position="2227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2242"/>
            <end position="2256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="2557" mass="271861" checksum="0DC36286E1A02E8C" modified="2014-01-22" version="1">MLTATLVGLNVPASAFAVPGPGMDRGDAVVELPDVPKSTEVGQDEDAEANLTTADEVPVDPYAPTAVTPWQQDSGVVDLTGIAPGESKPVDDLPIALGVPEGTDPADLAGAWTVGLAAPETSQDAGVPGLILKLTPPAAAAPEAAVALSVDYTAFADLYGPQAADRFGLMLLPDCVYDAPGTGDCAPVEGEEEDPAAAPTAQRLSSEVELVPAKSSVMRTLATSNASTRRIVTGSVPVGKLLDDSTGTGGVGGMARAAAESGSGVVGALDTGASSSGDFTATPLLSSGSWASGSSSGAFTYSYQVQVPETAGGLTPQIGLSYSSQSVDGRTSASNNQASWIGDGWDYSAGSITRTYANCTQDSKKAGSSNAKHRTGDMCWGSKNATLSLGGSTTELVWDESKDKWFTANGDGSVIEQKFDTSKSNGDKDGEYWVVTTRDGTKYNFGLNKLPGWSADAAVTNSVLTVPVYGNHAGEPCYQGSTIDDFKKSACTEGWRWSLDYVEDINGNAMSLWWAKEQNYYAKNFNFKSPVKYDRGGYLTRIDYGQRKDSVFSAAPQGQVSFAVDERCYTEGSVTCTDANFASKDPGKYRVWYDTPADLRCESKKMCWNAAPSFFTRKRLDKITTSTQRVQGSTARQTVDEYQLKQSFPVLKTGPNTALWLEKITRTGFGVGGDKIALNPVSFESNAEDMPNRVKRDNRPGFSRLRIARVINEYGGETVVSYKAPEGDCKTGTGLPGKGDTAELKSNTRLCFPAFWHPDPAAEDIDWFQKYVVENVEELPNVDGAFSTVTNYAYKGAAWKLAEQEFTKKATRTYSEFGGFEQVTTLTGAEDAAIGSKKTKAVARYFRGLGDTVAVKDIAGNEIAKDEDPFAGRIAEELTYSSAGDADTDWLTRNVTFPAATELGQRDRDGISPLRAWRITEPRQLTYTKSSGTGDDNRTQRSIETKTTYEATYGLPTRIESLGDTGRTGDESCTSLEYVHHTGKNVIGLTKQVRTSPTTCAAATFDTLKSLTSASRSSYDGGAYGAALTDQTRGLATETWTLNGDATGFQSDGTTGFDSIGRVVRRTDADAKVSTITYEPATGQAYKVTEKNSLGHDQTQVLDPGRAVGLKVTDPNGHVSEAKYDAMGRLVEAWAPGRVPSGSAVPDFKAIYNTPVGKPPFVTTQTRGHENRVETSVTLYDGLGRERQTQEQATGGGRLITDTLFNSSGEVWQTNNAYYTAGTPSGAMFTPLADTAVPNATRYTYDGLGRVMRELPILRGEEYPARATNYEYGTDNSTVINPAGAASYRLFTDALGRTTRVDTFSDANRAAFTSMRYQYDARGLLAKATHSADTARPWSWTYDQRGRMETATDPDAGTTRTTYDNRDRPVTVANDRGVTIWNAYDELSRPKQQRLGNSTGALLSEYTYDTAAGGKGLPATATRYTDGLPYTQSVGGYTRDYQPTSTTLSLPQSIADTWGLRSSYTYGYSYTDTGLPESATVPAIGNFTDEKLLVRYTKDGLPLSVSGKDWYGSETVYSPHGQVLRSTLGAQPYRVWAMSSYDDASGALTQQQVFREGTTDKTVVGGNLVSNRSYGYDEAGNVTSIRERSTGIQERQCFWHDKVGQMTKAWTSKDQETCAAGPVNTDGSANVAAGPDNSGYWQEYEYDLLGNRKKLVEKDLTGNAAKDATTAYTYGKTDGSQPRTLTKVTKKYVTPAGAAVTAEANRLYELTGETKSVTSVETGDKQDLTWTYDGKVERITGQGSNGKTAYVGLANKCLDLKSSMPTAGQPIQLYTCNGGLAQKWRFSATPNQTDANLGTLSIHDGWCLQPAANTAGSAFGLQKCDGSAAQQIKRNLATNQLTHVGSGLCAAVKDAVTTDATPIVLVACAATSTAQHWQAQNETRHIYGPDGTRLLTLQGKQATLMLGEAQVTVQQGGVLVNTQRSYAAPGGSVVRNAYGAGASGLVALAADHQGSPYAEIALNSTMQVRIRKQDAFGNQRGTVPLGMHIASNDGFLGATRDDASGYTPLGARLYDPVVGRFLSADPILDLADPMQSNGYAYAHNNPATMSDPSGLSISLTPSEMASALAGAGLSAAQVADAQANSSRSLTSVILSAAWSMLKDFIGITDAMNCFGGDMWACGSLIIGAIPWAKLGKIPAVLRAIDRTIAAIQAWKTAKRAAEAVLAAARAAERAALNAKKMAIERAKKAAQAAKKKAADKINTTSNNAVNAAKKTGNPVQKQAQAKANPKGASAAGGGNKGQSKPGGTSGGSARSNGGASGGGGAGKAGDSCPINNSFTPGTKVLMADGSKKRIEDVKPGDKVKTTDPKTGETRIETVTAAIKGNGVKHLVKIVVDTDGKRGKKVAEVTATEGHPFWVPELSRWVDAADLLPGELLRTGAGTTVQVTAIKRWTAPRATVHNLTIGNVHTYYVIADNQSLLVHNCGGSGNTNGRPHSSKCECASGADPRIPNNPYGGRGKPATQAQHDELRDELMDANPGWKHVAGGRDRNTGDILPEVAESQANGRRADLKFEMEDGSPVFVQTVTTYKNGAMIMEEFEAAHDLSKLGLVITVPKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTM8</accession>
    <name>U5VTM8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulatory protein uhpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_03780</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357990.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03780"/>
    <dbReference type="EnsemblBacteria" id="AGZ39045">
        <property type="protein sequence ID" value="AGZ39045"/>
        <property type="gene ID" value="AFR_03780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.763"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="134"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="202" mass="21126" checksum="3FB512B0F4636DEA" modified="2014-01-22" version="1">MIRILLIEPMNLLRGALAATLSLEEDLDVVAELDTLDQAPAMARAVPPDVAVVNVALLAGDGLDTIARLTAEQPGCATLLLAGPDDSGLLNRALTLRTDGVVGTQAAPCELVRGIRRLVRGERVIDASLAVAMVAAPRSPLSPRELNVLSVAASGVPSTEVAAELHLSTGTVRNYISAILRKTGARNRLEAVRLAEGAGWFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTS8</accession>
    <name>U5VTS8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359869.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09615"/>
    <dbReference type="EnsemblBacteria" id="AGZ40212">
        <property type="protein sequence ID" value="AGZ40212"/>
        <property type="gene ID" value="AFR_09615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1960"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_2_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="20067" checksum="EE0323B4DE973D0D" modified="2014-01-22" version="1">MARWEAGARERLVLAAVDLFTEQGYDTTTVAQIAERAGVTKSTFFRHFPDKRELLVAGQEALSRLLSEGITDAPGDASPLEAVAAGLERASEAMGPMNRELAPRLKAAIASSAELQERDALKSVSLAAAMTSALVARGVPGPTAALAGELGLLAFKRGYAVWSEGDRAPEDQLAPHALAALDELRAASASLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTU6</accession>
    <name>U5VTU6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ftsZ</name>
        <name evidence="11" type="ORF">AFR_10025</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="6">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360031.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10025"/>
    <dbReference type="EnsemblBacteria" id="AGZ40294">
        <property type="protein sequence ID" value="AGZ40294"/>
        <property type="gene ID" value="AFR_10025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2043"/>
    <dbReference type="eggNOG" id="COG0206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_11"/>
    <dbReference type="OrthoDB" id="1009041at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 6 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="9">
        <location>
            <begin position="10"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="10">
        <location>
            <begin position="204"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="318"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="330"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="18"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ40294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="378" mass="38933" checksum="8BD5105285567F8F" modified="2014-01-22" version="1">MTPPHNYLAVIKVVGIGGGGVNAVNRMIEVGLKGVEFIAINTDAQALLMSDADVKLDVGRELTRGLGAGAQPDVGKNAAEDHRDEIEEVLKGADMVFVTCGEGGGTGTGGAPVVANIARKLGALTIGVVTRPFSFEGKRRQVQAEQGIDELRNQCDTLIVIPNDRLLALGDRGISMMDAFRQADQVLLSGVQGITDLITTPGLINLDFADVKSVMSGAGSALMGIGSARGDNRAVEAAEKAISSPLLEQSMDGARGVLLSIAGGSDLGLFEINDAAQLVTDAAHPDANIIFGAVIDDALGDEVRVTVIAAGFDGGTPSYKPAEAPVRNKVPAPASPPAVTPTAPVPVTPPSPPAQTTPPPRRVLFDDVDVPDFLKNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTU8</accession>
    <name>U5VTU8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FeS_assembly_P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10695"/>
    <dbReference type="EnsemblBacteria" id="AGZ40428">
        <property type="protein sequence ID" value="AGZ40428"/>
        <property type="gene ID" value="AFR_10695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2180"/>
    <dbReference type="eggNOG" id="COG2151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="2">
        <location>
            <begin position="57"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="159" mass="16534" checksum="C7BCF31857FDE529" modified="2014-01-22" version="1">MTDKTVEEIASDAADTAGPDDATAPVVAETDAAPVSADAGTAPAGESGVVVSKATIDEVEEAMKDVVDPELGINVVDLGLVYGAHVDDDNVVTLDMTLTSAACPLTDVIEDQTRQALTTGPGGGLVKDFRINWVWLPPWGPDKITDDGRDQLRSLGFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU16</accession>
    <name>U5VU16_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360168.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10375"/>
    <dbReference type="EnsemblBacteria" id="AGZ40364">
        <property type="protein sequence ID" value="AGZ40364"/>
        <property type="gene ID" value="AFR_10375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2114"/>
    <dbReference type="eggNOG" id="COG3247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054698_0_0_11"/>
    <dbReference type="OrthoDB" id="1615682at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="416" mass="42199" checksum="699C482421E6A637" modified="2014-01-22" version="1">MLWSGSSPLSVVRDGMWGFLLFAGVAWLAIAWSVLRLEPAGVADVAGPVILFGAVTEVLRALAGTRTWWLNAGMAVLFAATGVIMMADGGGAWTTPAALIGWYLMVRGAADLAISMLTRESDRIWGLLMVVGVAEIGLGFFAASSFARTAEMVIVILGGAALARAVADLVAALRLREVSVAARAERLLELTPERAIGVAGYSAGLSDFEAGQPERSGRARHRAMPRTTTTTMAGQDASGPVPGTQLAAPEGYGAQGSQISGPLGSQISGAQGSQISGTQGSQISVAQGSGAQIGGAQASGQVQLSDEAQGSFHDEVLRTTADLDAMLALAGVTGAAVPGAAARAAADEQVEVPDTAEGAEVPAPEEVAHASAVARRQGPAQDDGSLPSLDAAARAAETASPGMDDTAIIARSRMVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU64</accession>
    <name>U5VU64_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358126.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04385"/>
    <dbReference type="EnsemblBacteria" id="AGZ39166">
        <property type="protein sequence ID" value="AGZ39166"/>
        <property type="gene ID" value="AFR_04385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.886"/>
    <dbReference type="HOGENOM" id="CLU_2314080_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="99" mass="10295" checksum="F219EE2CE252A093" modified="2014-01-22" version="1">MNLGTSTLLVGGVIGLLLAVFGVKMLVTGRGPASTLRNFPGVRAAAFYHLLFGLALLLMVLGQTLLGGPARVVVSGVAIVLVGVALVKYRPRVRKPVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU70</accession>
    <name>U5VU70_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360277.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10650"/>
    <dbReference type="EnsemblBacteria" id="AGZ40419">
        <property type="protein sequence ID" value="AGZ40419"/>
        <property type="gene ID" value="AFR_10650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2171"/>
    <dbReference type="eggNOG" id="ENOG5033IPK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150079_0_0_11"/>
    <dbReference type="OrthoDB" id="1759285at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="98" mass="10650" checksum="3B6F2E29290CCF81" modified="2014-01-22" version="1">MAEGSDTTLTADIKTFAAGHGGAKAVIEYVGKRGARIVLVGEDGEWTDQFAADGTAAARAACKTAGVEVENEWERELMEQMRPSNDLWRSMSRRSMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUE6</accession>
    <name>U5VUE6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[Ribosomal protein S18]-alanine N-acetyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.266</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_04860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acetylates the N-terminal alanine of ribosomal protein S18.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + N-terminal L-alanyl-[ribosomal protein bS18] = CoA + H(+) + N-terminal N(alpha)-acetyl-L-alanyl-[ribosomal protein bS18]</text>
            <dbReference type="Rhea" id="RHEA:43756"/>
            <dbReference type="Rhea" id="RHEA-COMP:10676"/>
            <dbReference type="Rhea" id="RHEA-COMP:10677"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64718"/>
            <dbReference type="ChEBI" id="CHEBI:83683"/>
            <dbReference type="EC" id="2.3.1.266"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. RimI subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.266" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358302.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04860"/>
    <dbReference type="EnsemblBacteria" id="AGZ39261">
        <property type="protein sequence ID" value="AGZ39261"/>
        <property type="gene ID" value="AFR_04860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.981"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_3_11"/>
    <dbReference type="OrthoDB" id="1390922at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006474">
        <property type="term" value="P:N-terminal protein amino acid acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="16318" checksum="022195FF89A83296" modified="2014-01-22" version="1">MEITRFRWWHITEVLALEEDLFGPEKWSAAMFWNELAQRHFYLVAEENEKVLGYAGLAVVDQDESWVQNIAVDRGAQRRGIGRQLLEALLAEAGKRKVLLEVAVDNAPAQRLYATYDFEPVGLRRGYYQPSNTDALVMMRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUF9</accession>
    <name>U5VUF9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11100"/>
    <dbReference type="EnsemblBacteria" id="AGZ40509">
        <property type="protein sequence ID" value="AGZ40509"/>
        <property type="gene ID" value="AFR_11100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2261"/>
    <dbReference type="eggNOG" id="COG0613">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066403_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021447">
        <property type="entry name" value="DUF3097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11296">
        <property type="entry name" value="DUF3097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="28480" checksum="B98312293C7EFD5C" modified="2014-01-22" version="1">MAGNWRRRKVIPEVDAERDLVVEDVDSGFCGAVVGFEFGAVVLEDRHGKRRNFPLEPAAFLLEGQTVTLRKPVAGPAPAARQRTASGSVAVAGVRAQVAKASRIWVEGVHDAALVERIWGDDLRIEGVVVEPLDGIDDLAGAVRDFHPAPGRRVGVLVDHLVPGSKESRIVAAVKDPNVLVTGHPYIDVWQAVKPERVGLKAWPVIPVGQPWKEGVCRAVGISEPYEMWRRILGSVNSYKDVETPLINAMERLIDFVTEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUP5</accession>
    <name>U5VUP5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12085"/>
    <dbReference type="EnsemblBacteria" id="AGZ40703">
        <property type="protein sequence ID" value="AGZ40703"/>
        <property type="gene ID" value="AFR_12085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2460"/>
    <dbReference type="HOGENOM" id="CLU_1850855_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="15185" checksum="48C64D6269C6D914" modified="2014-01-22" version="1">MLFHRERLERFEGRHSVDQTDKVEPVSKARPGEGRTKGSPILPAGQQRPVPHRSDPGRQRRAGVSAVVDSVVGFAGHPFAVPHRFHHVQPGQLPVRHGLRARTCSFGRLVRPGSWLGLGCRRAAQSEEFHELIAVGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUQ4</accession>
    <name>U5VUQ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport system inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_11400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11400"/>
    <dbReference type="EnsemblBacteria" id="AGZ40569">
        <property type="protein sequence ID" value="AGZ40569"/>
        <property type="gene ID" value="AFR_11400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2324"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_3_11"/>
    <dbReference type="OrthoDB" id="1619224at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="107"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="35899" checksum="27B5E3B552B19FC4" modified="2014-01-22" version="1">MSISSTTPGAAATTEQTAEETGQARVATRNRRGKAPLSEGKRAERKLGWLLCAPAALVMVAVTAYPILYSFWLSLQRYDLKFPDERQFVWFENYATVLSNSYWWTAFGVTMLITVVSVVVELVLGMGLALIMHRTIIGRGLVRTSALIPYGIVTVVAAFSWRYAWTPGTGYLANLVAPDGAPLTERASALAIIILAEIWKTTPFMALLLMAGLALVPDDLLKAASMDGASGWQRFTKVMLPVMKPAILVALLFRTLDALRVFDNIYVLTNGANETSSVSMIAYNNLMKGLNLGIGSTMSVLIFLTVAIIAFIFVKLFGTAAPGSSDDGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUQ8</accession>
    <name>U5VUQ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359001.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05905"/>
    <dbReference type="EnsemblBacteria" id="AGZ39470">
        <property type="protein sequence ID" value="AGZ39470"/>
        <property type="gene ID" value="AFR_05905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1199"/>
    <dbReference type="HOGENOM" id="CLU_2340493_0_0_11"/>
    <dbReference type="OrthoDB" id="1918912at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="10945" checksum="9D94A4052E9AE50F" modified="2014-01-22" version="1">MKSRLRRLHLDGRVFTWKGEVRHAGTRRFIRVRAWGAGKNSRPLQADLHERPNQTYPYPTADDVRTLVTHALAAGWTPEAHGAAFVLTNVSLPAFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUR7</accession>
    <name>U5VUR7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05535</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05535"/>
    <dbReference type="EnsemblBacteria" id="AGZ39396">
        <property type="protein sequence ID" value="AGZ39396"/>
        <property type="gene ID" value="AFR_05535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1123"/>
    <dbReference type="eggNOG" id="ENOG5033HJ6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167344_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12568" checksum="816D1C6C9879A70C" modified="2014-01-22" version="1">MNFVVAVNNFGDTRSGGLAGPMGLFVIVLMAIGTVLLIRNMNKRLRRLPDSFPDPAAARREAEITRLNADLERPDADPRAAEAGDQASDVEIRPAEAEVKATESVDAPPADGARGDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVN2</accession>
    <name>U5VVN2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359227.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07035"/>
    <dbReference type="EnsemblBacteria" id="AGZ39696">
        <property type="protein sequence ID" value="AGZ39696"/>
        <property type="gene ID" value="AFR_07035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1435"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006462_2_3_11"/>
    <dbReference type="OrthoDB" id="1573900at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="1">
        <location>
            <begin position="16"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="543" mass="59972" checksum="1BFFF75E38EB4953" modified="2014-01-22" version="1">MPSPTDLWWRSAVIYQIYPRSFADSNGDGMGDLPGITARLSDLRDLGVDAVWLSPFYPSPQHDAGYDVADYRAVDPLFGDLGDADKMIAEARTLGLKVIVDLVPNHTSNEHVWFQAALAAGPGSPERERYIFREGKGADGSEPPNGWQSVFGGPAWERVPDGQWYLHLFDVSQPDLNWGNPEVRAEFVSVLRFWLDRGVDGFRVDVAHGLIKDATLADWTYAGEILGGLAPEGAPPPPMWDQDGVHEVYQQWRSVLDEYDGERILVAEAWVQPAERLARYVRPDEMHQAFNFEYLDAPWSADPLRKVIDSTMAANASVGAPTTWVLSNHDVVRHATRLGYPVGEPRKHGIGIGDPQPDVGLGLRRARAATLQMLALPGSAYLYQGEELGLPEATEIPDEFRQDPAWERSGHAERGRDGCRVPIPWEADAPSYGFGPADASWLPQPAVWAEYALDRQRGVTGSTYELYRSALRLRAVHALGIGALSWIDTPADVLAFRNGDLLVVTNYGDAPAELPLNARVQLSSEPLTEDGRVPQDVTVWARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW64</accession>
    <name>U5VW64_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08430"/>
    <dbReference type="EnsemblBacteria" id="AGZ39975">
        <property type="protein sequence ID" value="AGZ39975"/>
        <property type="gene ID" value="AFR_08430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1716"/>
    <dbReference type="HOGENOM" id="CLU_2033084_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="12381" checksum="CF3D861F362808BE" modified="2014-01-22" version="1">MVSILLICFGAVAYVHFRPRTAGRPDDLGGAPATVIAPAPAPAPQSLVVPPPAPAPVIVRPEAARTVPQERPAEVVRTRVAPPAPVAAAPAVRRGRANSGFNRFAGAPAQARVSASRSRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWF0</accession>
    <name>U5VWF0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="9">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">argS1</name>
        <name evidence="9" type="synonym">argS</name>
        <name evidence="13" type="ORF">AFR_08510</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359522.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08510"/>
    <dbReference type="EnsemblBacteria" id="AGZ39991">
        <property type="protein sequence ID" value="AGZ39991"/>
        <property type="gene ID" value="AFR_08510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1732"/>
    <dbReference type="eggNOG" id="COG0018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_5_1_11"/>
    <dbReference type="OrthoDB" id="1146366at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arg_tRNA_synt_N" evidence="12">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="DALR_1" evidence="11">
        <location>
            <begin position="442"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ39991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="552" mass="59191" checksum="1DFF8ECB4B26D3E1" modified="2014-01-22" version="1">MNLEALLSARLAAAFSQVAGVTVDPAVRRSQHADFQSGAALPLARELGRAPRDIAADVLAAADLTGVATGEISGPGFLNLRLDDTFVTTAANDVAADPRLGVPLAGHPERVVVDYSSPNVAKELTIGHLRSTVIGDAAVRVLEWLGHDVVRANHLGDWGTAFGLLIEHLGADDAAGIDDLTAFYQAARVRFDTDEEFRTRSRLRVVALQAGDEATLAQWRQLVERSKRYLLAAYERLGVTLGPDDFLGESFYNDRLESVVDDLAAKDLLVESGGALCAFPPGFTGRDGEPLPLIVRKSDGGFGYAATDLAALRYRVEELGATRLLYFVGTPQRVHFQMVFAVGAAAGWLPDGVVAEHVGFGSILGTDGKMFRSRTGESVKLAAVIDEAISRAAELAANPEVARATGIGALKYADLSGDRLSDYTFDWDRMLALTGSTGPYQQYAYARIQAIFDRAGPFEGRIDVVEPAERALVLELLAFAPVVTGVGRSLEFHRLTGHLYAVAQAFSVFYQQCPVLKAPAGVRESRLALCALTARTLRRGLDLLGIATPARM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWZ9</accession>
    <name>U5VWZ9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_16180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361593.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16180"/>
    <dbReference type="EnsemblBacteria" id="AGZ41518">
        <property type="protein sequence ID" value="AGZ41518"/>
        <property type="gene ID" value="AFR_16180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3283"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_476225_0_0_11"/>
    <dbReference type="OrthoDB" id="1698403at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="63"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="572" mass="61119" checksum="D8EC4E284C81FBD1" modified="2014-01-22" version="1">MNDPGASVTSVTSATLDSSGASDQPGVMPVVAAADVVGHGAPGPGAAEPNGWWVGTNEDPDRYELLGAGLSGGEGTTFAALYHGEQGTTPIRVAVKQLHRPAGAPSTWPQPADWTRWQDQIQIIHQVSSPHLVQVRTILTGAPPHRRGALADTAGQARYEIPYLIMEWVSGSTLQRRVRAVTGTRDARARIGWIAQLGEAVSALHSATRTAGNPMVHRDIKPANCMIDDNGRLVLVDVGAMRRTDSSYDARGLHSRRYTAPEVLADLHGPRSAASDVYSMAAVAYFCLTRQDPPEHGHLTPRRIRAAIDAPRRWRRQLAVHLAPALSPRPEERASIGPDAWARQLLRITARRRKWPAALGLTLAVLVAAVVSLYATGTLTIESVATAPGGSTQSAAGMRAFGGSYQDFAFTAGDSSLFIEPPADYRHLWGGYLPAQRCATTVEFDVVLTGTPRLPAYGLAVAPRSALVDDQPQGASIQFEYETPELTSRPGSYVRPATLPGGAWSIAVRPTPAPDLRLARHVRIYAVGSTMAIDIDGRQIAEYDLSDVECGGAAIRVWGAAFTFTGLSVRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXA4</accession>
    <name>U5VXA4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">von Willebrand factor type A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10355"/>
    <dbReference type="EnsemblBacteria" id="AGZ40360">
        <property type="protein sequence ID" value="AGZ40360"/>
        <property type="gene ID" value="AFR_10355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2109"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018489_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="393"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="591" mass="64064" checksum="153A7BE3557D0069" modified="2014-01-22" version="1">MSRRTMIPIAAVAILLLVVLGGYKLIEKDPACAVTLHVTSSTEKDELVASLAKDYNAARRELRGGGCARVEAAGETSGRTKSALAEHWKGHKGSRPDVWLPSSSMWVDLLRQEEPDEAPATTRWASIAQSPVVVAVPRDLAAGLGWPAKRFSWAQLLEPRPGRSFYVGREDPRTSTSGLASTIAAYYAATSGELDAAKLEEPAVQAYVRRVEANVVQRGFVQDDIMNLLRDQLGIDQGQASTDAPISAIVLQEQLIHLYNTMNLEADPQRLRSGVHESPNQPLVPIYPTDGTMMLDHPYVVLPSAEEDGAVRDAADDFRAFLRADPARKLFLAAGFRDADGRAGQAMTAVTPVPANARQVGDGRTGLLIRDSPDAATIQAVLANREGLKKRAQLLMVMDESGTMDRPAASGTEVTRGRAAQDAAANSVQLLDPRDRVGLWFFHSLGNPPRTEKLPVTELGDDDGFRVALRDLKPTKDKDTALYVSVRDAHRRMVRDYRPDSINAVIVLTDGVNDYDKSRYSLDQLVRDLKASDRTRPVRVYCIAFGETAAGGKAALDRISGATGAGRARDALDPAAVEKVFGDIVRDATKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXF6</accession>
    <name>U5VXF6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglucose isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360270.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10630"/>
    <dbReference type="EnsemblBacteria" id="AGZ40415">
        <property type="protein sequence ID" value="AGZ40415"/>
        <property type="gene ID" value="AFR_10630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2167"/>
    <dbReference type="eggNOG" id="COG0144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036298_0_0_11"/>
    <dbReference type="OrthoDB" id="417261at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="481" mass="49908" checksum="2EB7667CF1F5B8CA" modified="2014-01-22" version="1">MTDDLLAGVEAAAGFAVFGGSPAASRRELVARGIPGLLASKDPTLWGPAAEAGAKERLAFLDDHRHGLELLPLIAELHEELGDLTEVVLAGPGGTAEVAARTLGLRLTVLDDADPHRVRALIDDAARLRRTLIVLRDDSLHPVLRQAYLDLGLSPAEAGRHFVTLTDTEIPGALAVPGDSGALTALAFAGADVAELIDEAELFAPSLAADEDNPAMALGLALAGARQIMLVADGSSLESVGDWVAPLLTDALGVLTLVVEPPGERHEDVLTISYGGSLPPAAVAGGPLRPDLAVNGPLGAHFLAWPYAAALAAHVRGVDPFRSYPGEPAFDDPATPSFTDGTVEIFTTGAAGNLAEALRELLAGVDDHLAVQAFLDRDGDAAVARIRPLLAAACGRPVAFGWGQYPRCGPQHGSFLQITGAVTGDVPVPGGPETLGGRQAARAAADRHTLTRCGRPLLRLHLTDRTEGVRQLLAAAEMLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXG2</accession>
    <name>U5VXG2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361543.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16000"/>
    <dbReference type="EnsemblBacteria" id="AGZ41482">
        <property type="protein sequence ID" value="AGZ41482"/>
        <property type="gene ID" value="AFR_16000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3247"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084695_0_0_11"/>
    <dbReference type="OrthoDB" id="1445278at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="3"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="30072" checksum="D75F30CBB1F9014E" modified="2014-01-22" version="1">MTSIVIAAHNEAAVIGRCLDALLADAAPGEFDVTVVANGCTDDTAAVASARPGVRVVELETAGKVPALNAGDAVAVGFPRVYLDADIVLSTAAVRSVADALDDTALAATVGRELDLTGRPLLVRAYFAVNGRLPVFRDGLFGRGVMAVSEKGRARFGTFPELVADDLFLDSQFGSDEKRHVTSFAARVATPRRTGDLVRRLVRVRGGNAAMRAAAANGQVAAPVRSAARMSWLRDVVLPRPWLAPAAVCYVAITVVAARAARRAGDGGAAWGRDESSRRVESPAGGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXI4</accession>
    <name>U5VXI4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40376">
        <property type="protein sequence ID" value="AGZ40376"/>
        <property type="gene ID" value="AFR_10435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2126"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018334">
        <property type="entry name" value="ArsR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00846">
        <property type="entry name" value="HTH_ARSR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="113" mass="11978" checksum="E6C1749E1D22C47D" modified="2014-01-22" version="1">MTPSSSAWAPLARRPLDADQARAFAPMFKALGDPVRLRLLSMIASAGGGEVCVCDLTGSFKLTGPTISHHLKVLREAGLVDSDRRGTWVYYRLVPAALTLLAGLLDVSSVPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXU8</accession>
    <name>U5VXU8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360495.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11230"/>
    <dbReference type="EnsemblBacteria" id="AGZ40535">
        <property type="protein sequence ID" value="AGZ40535"/>
        <property type="gene ID" value="AFR_11230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2287"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_44_1_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039538">
        <property type="entry name" value="BetI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13977">
        <property type="entry name" value="TetR_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="21782" checksum="0EAB7D68B73B8D0A" modified="2014-01-22" version="1">MTDAPETTRRGAYDKGVRRRREILDRAIEVFAERGAEGTSLRAIGEAIGVSHAALKHYFASREELLIEVYRAAEERSMTEDRPPADSTVVGFMTHAARRNHGVPGLVQLYSTLVAGALEEGHEPSRAFFGARFADLRRFLASRVEAEQKAGRIRPDIAPEAAAALIIAASDGLQTQWLLDPRVNIETSLELLESLLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXW5</accession>
    <name>U5VXW5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phytochrome-like protein cph2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_17800</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362149.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17800"/>
    <dbReference type="EnsemblBacteria" id="AGZ41838">
        <property type="protein sequence ID" value="AGZ41838"/>
        <property type="gene ID" value="AFR_17800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3610"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_50_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="258"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="398"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="675" mass="72550" checksum="F70CB39333336558" modified="2014-01-22" version="1">MWRRQNVRRRSGTGSRLFLAYAVASLVLVLLLGVMLVRSYRDDAEHQGREQGRAQASVIEEMAVAPALDGAELAAGLTATQLERLQGATDLAIFHGSVVRLRVRSYTGQVVFSDDGSTAGGISVSSAAFRAAVAGQVDIAVVADSQRSAGKVIRVLQPVIAGASGQAIGVLELYLPYDAIDAQLQRQMTATYWRLGVTLAILYLVLALIAWSTTRSLRRHAARREHEALHDGLTGLPNRAAFRERARTAISVATGSGGEGAIVLVDLNRFKEVNDTLGHHAGDELLRLVGSRLGQALRTGDTVARLGGDEFGLILPALPGQDVLDLLEEARRELTRETVLDGVPLSIEASFGVALYPHHGTEVEELLQHADAAMYQGKRGAVDVVLYAEGGVANPTHWLMVQAELRHAIDRDELVLHYQPKVRFADDEIGGLEALVRWQHPQRGLLPPSEFLPAAEQSGVIEVLTAWVLRRALEDQAGWTALGQSWPVAVNVSARNLEAPGFPQFVAGLLAEHSTPPGQLLLEVTETALAGDAEIVARAVVELRALGIGVAVDDFGIGYTSLSQLRTVPVSEVKIDRLFVIDLDQDPQNQAIVRSVIELAHGLGCRVTAEGVETAQVSAWLAEAGCDDAQGYLYSRPVVWPHLLDLFLHPRSTGRHAALDEPAAPAQLTTGGPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXZ3</accession>
    <name>U5VXZ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362209.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41868">
        <property type="protein sequence ID" value="AGZ41868"/>
        <property type="gene ID" value="AFR_17950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3641"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_1_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="9"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="28960" checksum="E355F71695A1D89C" modified="2014-01-22" version="1">MARQELARFLRDRREGLRPADVGLPAGPRRRTPGLRREEVAGLAHISVDYYVRLEQARGPHPSGRILEALTGALRLTTAERNHLFRLAGASPAPPGAPSRQVRPYVADLLRRLPGSAAIVTDATYDVIAWNPLAEALLGDLGSRPNLARRRFLEPGREYGPGAADFAEIAVARLRSSAARYPHDTGLSRLLTELWDGSPEFRAVWETNPVRVPGHRTKTIEHPVAGPLKVNCDVLTVPDDDQQVVFVTADPGTPAARALRLLSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY54</accession>
    <name>U5VY54_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11360"/>
    <dbReference type="EnsemblBacteria" id="AGZ40561">
        <property type="protein sequence ID" value="AGZ40561"/>
        <property type="gene ID" value="AFR_11360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2315"/>
    <dbReference type="eggNOG" id="COG1452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005386_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="PTHR10412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="871" mass="98114" checksum="F1A872B01DB911F0" modified="2014-01-22" version="1">MGDGVAERERLAQADSGERPWRAWGPYLAERAWGTVREDYSEHGTAWDYFPHDHARSRAYRWNDDGMAGVCDDRQTFCFALALWNGSDPILKERMFGLGGDGGNHGEDAKEYWWYQDSTPTHSWMRWRYHYPQAAFPYDELVRVNGHRSREEPEYELIDTGIFDEDRFWAVTVDYAKADVDDMCIVVTVSNRGPDEATLHVLPSLWFRNTWSWGLPNRPVPVIEGSAGKLAGEHRSLGHITLEGEGEPPALLCDNETNSQRLWGLPGTSLYPKDGINDHLIAGAPTVNPAGVGTKGALHYELTLGPGETRQIRLRLTRSDEAATLDLGDDFAAVVRAREDEADAYFAELIPAAASAEEAAVARQGIAGLMWAKQFYHFDVKQWLQGDPGSAPPPPGRRYGRNNAWWHMNSFDVISMPDPWEYPWYAAWDLAFHCVSIARVDPGFAKAQLLLLLREWYMHPNGQIPAYEWAFGDVNPPVHAWAALQVFEIDGGRDHDFLARIMHKLLLNFTWWVNRKDVGGNNVFEGGFLGLDNVGPFDRSAALPVAGVLEQSDGTGWMAMYALNLLDMSIRLALHDRAYEDMATKFFEHFAYIAEAAYRQGLWDDEDCFFYDVLRMPDGHKMPLKARSIVGLLPLAATTRLSAGTLNRLPELSARLRWLLSNQAEYADVISARRLSPDGRQQRLLSMVGQDQLLRILARMLDPEEFLSPYGLRTLSRAHLEKPFTVSLGGSDFTVGYEPAESTSGLFGGNSNWRGPVWMPVNYLLIEALREFAAFFGPDLMVEYPTGSQTKMPLSAVADDLSRRLISLFVPDSWGRRPIYGASEVFQNHPDWKDLIAFPEYFHGDNGAGLGAWHQTGWTALVVDLILTLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY83</accession>
    <name>U5VY83_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SARP family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18400</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AfsR/DnrI/RedD regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362331.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18400"/>
    <dbReference type="EnsemblBacteria" id="AGZ41958">
        <property type="protein sequence ID" value="AGZ41958"/>
        <property type="gene ID" value="AFR_18400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3734"/>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004665_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15831">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005158">
        <property type="entry name" value="BTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03704">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01043">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="27909" checksum="C92C528637BB1AAD" modified="2014-01-22" version="1">MLEFSVLGPLTLRRDGRALTVPTVMLRRVLAVLLARSGTPASVDLLIDELWGERPPRSARRTLSAYVSRLRKLLGDDDRVESSPGFYALCPRPGELDAEAFRQLTERAAQAHATGDRPAAAAAMRSALALWRGRAFDGQRDTALVERTAEGLEQLRLSTFEEYIDFKLDAGATGDLVGQLDAMIGEHPYRERLRGQRMLALYRGGNRADALETFRRTHHVLVQDLGVEPAVKLQQLHQRMLAGDPTLASSGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYL8</accession>
    <name>U5VYL8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ankyrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360815.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12350"/>
    <dbReference type="EnsemblBacteria" id="AGZ40756">
        <property type="protein sequence ID" value="AGZ40756"/>
        <property type="gene ID" value="AFR_12350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2507"/>
    <dbReference type="eggNOG" id="COG0666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_48_1_11"/>
    <dbReference type="OrthoDB" id="1360387at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="31"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="99"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="202"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="276"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="344" mass="36126" checksum="598FA2BFB8B19D18" modified="2014-01-22" version="1">MTLQAAIRAGDAVAVGELLSTGTNPGHRDHDGRTPLMIAAGLGRLRLVALLLEAGADVFTVEPNMGATALHKAAQSGDADVIGLLLDHGALVDQQSARLGHTPLMDAVQHKNEAAVHLLLARGARTTIRNHWQETALDLALQDGADSIAGLLRARDDADASRVRALPLPAAVKAGDLPEVERLIAAGVPLDERMPEIGGHDDDYTALGLAARDGHAGIARVLRDAGADPRRLNGLMRATPGHEAAFAGHAEVLRVLTASGRTGAPALDLDAQGAYNGFTALHDAVWHGHGEAARALVEAGASLVLRSHTGHTPRQLALHYGYDDLATFLADAERHRYGRDSPHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYU2</accession>
    <name>U5VYU2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NmrA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360900.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12775"/>
    <dbReference type="EnsemblBacteria" id="AGZ40841">
        <property type="protein sequence ID" value="AGZ40841"/>
        <property type="gene ID" value="AFR_12775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2595"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_10_6_11"/>
    <dbReference type="OrthoDB" id="1616572at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="28174" checksum="34E3C629565CE713" modified="2014-01-22" version="1">MKILVTGATGTVGGLVARQLSGSGHEIVALVRDPARADLPAGVTLLQGDLTDPDAVRRALDGVDRAFLNMADDNGAVFAEVAAAAGVGHVVLLSSFTAVTDLPSGANNIITARHQAGEQALTAAGVPSTFLRAAGFDYNILMWAAGATDGVVRAPNTEVKLPVVDPADIAAAAVAVLTATDPRTGAFSITGPDSLSVRDQVSVVNETLGRSYTVQEISPAEAAAAAFPAGTPDFVTTSVLETMGPGAAVLTPADGVQSLTGNPPRPFAAWVADHAQAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYV8</accession>
    <name>U5VYV8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Alpha-amylase</fullName>
            <ecNumber evidence="4 9">3.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_19380</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
            <dbReference type="EC" id="3.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1" evidence="4 9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362520.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19380"/>
    <dbReference type="EnsemblBacteria" id="AGZ42148">
        <property type="protein sequence ID" value="AGZ42148"/>
        <property type="gene ID" value="AFR_19380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3934"/>
    <dbReference type="eggNOG" id="COG0366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013336_0_0_11"/>
    <dbReference type="OrthoDB" id="99080at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031319">
        <property type="entry name" value="A-amylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006046">
        <property type="entry name" value="Alpha_amylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM_fam20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00110">
        <property type="entry name" value="ALPHAAMYLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00632">
        <property type="entry name" value="Aamy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="SSF49452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665503" description="Alpha-amylase" evidence="11">
        <location>
            <begin position="34"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="CBM20" evidence="12">
        <location>
            <begin position="493"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="571"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ42148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="594" mass="62340" checksum="E80CD7600863288F" modified="2014-01-22" version="1">MRTRQVVAAATTTVLAAALLAVGVVRQGDQAEASTASPGPKDVIVHLFEWPWASVANECTTTLGPKGFGGVQVSPPQEHVVLPGSGYPWWQDYQPVSYQLKTRRGDRAAFASMVQTCHTAGVKIYVDAVVNHMAGGTSTGTGSGGSAYSHYAYPAVPYGTDDFHHCGRNGNDDIANYGDRWEVQNCELVDLSDLKTESSYVRGKLTAYLNDLVGLGVDGFRVDAAKHMPAADMAAFIDPVNGDPYVFSEVIEGGSGEPTPEEYQGIGDVTEFRYGDVVGGAFQNGSLSNLNNLASSMRLASGDAVAFVDNHDTQRNGKAKLTYKNGSSYALAEAFMIAWPYGVPQVMSSFTFSNNDAGPPAASNGTTNAVTCGSGWECEHRWRTTANMVGLRNAAAGAGVTNWWTNGSNQIAFGRGSTGYAAFNRSGSALTRTFQTSLPAGTYCDVMAGDFSGGSCTGPAYAVNSSGQVTATVAANSALALHVNARGSGSTTPPPASGCSTTFSANVTTTWGQNVFVTGNTAALGNWNPAAAVPLSSATYPVWTATVSLPGNTAVQYKYLKKEGSTVVWESDPNRTRTTGASSPCTATWNDTWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZX4</accession>
    <name>U5VZX4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ROK family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361329.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14940"/>
    <dbReference type="EnsemblBacteria" id="AGZ41270">
        <property type="protein sequence ID" value="AGZ41270"/>
        <property type="gene ID" value="AFR_14940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3034"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_3_11"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="386" mass="40207" checksum="F79EE1F6E1353B55" modified="2014-01-22" version="1">MATAGADLPRLRELNSLSVVRALRAHPAATVTELSQRTGLSRPAVDVIAQGLVTDGWASILEPGASSVVGRPARRYQFRAGAGHVLGVDVGVHKILALLSDLDGTIVHSVRHSVAADAGATERLAELDEVINACLTEAGKTPADIWGVAIAVTGAVDATGRTSFFTPLPGWKSVDLTAHLGGRFACPILVENDCKLAALAERWKGAAQDANDIVYVLGGMRTGAGLIIDGVLRRGFGGAAGEIGALKPIRWLQAPAHLQNCPGVPSTVGPDDAAAWVFNAARAGDKAARTAVNRYVKDLAVGTAALVLTLDPQVVVYGGGFSRSADLILDPLRREIHRLCLRTPEVRASTLGADAVALGALKLSLNEVDDRLFSAGLSAPVAPRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZY1</accession>
    <name>U5VZY1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42444">
        <property type="protein sequence ID" value="AGZ42444"/>
        <property type="gene ID" value="AFR_20860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4229"/>
    <dbReference type="eggNOG" id="ENOG503420E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138579_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="18624" checksum="E103F694AC8A8278" modified="2014-01-22" version="1">MEHRSGTMTGMPAETWTALTSLGGVALGGGLSWLVQSTTQRTNVRNEQRKLELARAETRRTERLAALERFVAAASEAERQALSRPRDQSAWDAWEAVTQPIMDRVWVAERMIRVLFPVPVHDAARAYFLVLNQGVWEGAEDYGAVRDSLEEPRVAFLDLAGDALEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZZ0</accession>
    <name>U5VZZ0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_20910</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20910"/>
    <dbReference type="EnsemblBacteria" id="AGZ42454">
        <property type="protein sequence ID" value="AGZ42454"/>
        <property type="gene ID" value="AFR_20910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4239"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="3">
        <location>
            <begin position="84"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="32406" checksum="A6C19D2898FF2214" modified="2014-01-22" version="1">MEHERHRPARRSTAWPVAGAALAVLVFLLVATGGEGPVTVVLPTAIAVTAAGVAAWAVHRTRADRAAYEARLTRWAASEAVLAERLRIARDLHDIVSHGLGLITVRVAAARLTAADPREPPGGPPAGPPAGPPAEPPAEPPAEPPADVLAGPPADVLADVLADVEAASREATAELRRMLTVLRSADDTAPRLPVDSLDQLPAIVAGTGASLRLEPVGEVSPGVQVAVCRTVREALTNVARHVGPSTVRVHVYRDHGSVVAAVSDTGPQGPWPTAPGAGHGLLGLHERVSALGGTLHAEPAGPGFRVTARIPDLAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W009</accession>
    <name>U5W009_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42474">
        <property type="protein sequence ID" value="AGZ42474"/>
        <property type="gene ID" value="AFR_21010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4259"/>
    <dbReference type="HOGENOM" id="CLU_2598124_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="79" mass="9219" checksum="338062684328E8A3" modified="2014-01-22" version="1">MFVSRFDPDFEDPANNSVRVIDGTLTKDERAKVELMDPKNRARYLLQKRIEEKGEMAVLLSQLQSQRHRTAMSVINNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0K8</accession>
    <name>U5W0K8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase, S-adenosyl-L-methionine (SAM)-MTase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363154.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22465"/>
    <dbReference type="EnsemblBacteria" id="AGZ42763">
        <property type="protein sequence ID" value="AGZ42763"/>
        <property type="gene ID" value="AFR_22465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4554"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_2_4_11"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="39"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="28009" checksum="FC6867E684C6CFF5" modified="2014-01-22" version="1">MGQVWQVGDNYEAYVGRWSRRVAREFVTWLGVPAGRTWLDVGCGTGALLAAALAVAPARLTGVDRSTGFLATARARVPEADLVAGDAAALPIEGGRAEVVVSGLTLNFVGVPARAAAEFARVAAPGGTVAAYVWDYADGMVMMRHFWDAATTLDPAASAFDEGSRFTLCRPGPLAELWTAAGLTGVETRAIDVPTVFTGFDDYWQPFLGGQGTAPGYVASLSEERRGALAGLLRERLPRADDGSIRLTARAWAVRGLATQARPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0N7</accession>
    <name>U5W0N7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulatory protein degU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_21385</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21385"/>
    <dbReference type="EnsemblBacteria" id="AGZ42547">
        <property type="protein sequence ID" value="AGZ42547"/>
        <property type="gene ID" value="AFR_21385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4339"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_316785_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="842"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="539"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="543"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="920" mass="93237" checksum="6739E6AF795C981C" modified="2014-01-22" version="1">MAQQDLAGLAAAHLRRPGLVMLTGAPGSGRSTLLRRIAATAPGPVYAGGGLAMLAAVPALALSRAVKARLPAHDVPLLAEAVHSRVRGGLLVLDDLQYADPATLAALPHLAEHIRVVVALRAPHRLPAETVAALRATATAWLDVLPLSPDTATDLARRAGGQLSDEALAAVVQRAGGNPLAITALSRQAGAGRGTPDTDIDQVAYAIATALADLPRPARTALAALGLLGRPAPAALLGPGADALREAGLVTEDGDGLVPVSAYFAETAAGLLDADARVDLHRALAALTPDAEAARHLAAAGDHQGAYRKALTAADRGGDRADLLLFACELPGVTVSSRVRIAAADALLSSGRPHAAARVLTTAAPLGVEADVLRGEALLQAGDPLGARAAVRPVPDASAALVVAARDRILLLADLATDPAAARDTAEKIAARYPAPPPGVAAALAAVRAAHRTPGWDTELAAAAGAGADPLTGRWSAWLLVEHLVADGRLTQAARVARESAAACAADLAYSWQTRFLAVADWSEVLQGTMIAASAAAATAPDDTPATGSSHAPRAWSSDATPAGSPPSVAATGTDMPAGTVASAAALAESVLPAGHAVPGTAGAGPAFAVPGTAGADPALAVPRAALTAQEAADRALRRAADLTDRALPADARGYAIAAAALVEADTGLLAAARGRLTAPDGHPATAWVAREAAWLDGQPDRAAQPPDLTTAGLLTGLRHITARWAAYDTGATARAAFASAFELDGPAPAIPAGLPAAARRTLTAWATGTGFTAAADGWSGTSLREQIRCLLAAGLHDTDPERAVAALLQAEHLADTAGLVVLAGRARRGLRRHHVHRDTRSPRSGTRLTRRETDVLRLVAAGEPTRRIAGQLGISAETVDTHIRAGMRKLGARTRTEAAALALTQIEASAEHPDREDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0W4</accession>
    <name>U5W0W4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PadR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363209.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22570"/>
    <dbReference type="EnsemblBacteria" id="AGZ42784">
        <property type="protein sequence ID" value="AGZ42784"/>
        <property type="gene ID" value="AFR_22570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4575"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_4_0_11"/>
    <dbReference type="OrthoDB" id="1706427at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="8"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="11180" checksum="AE679CB75057A1DF" modified="2014-01-22" version="1">MREPTYFILAALQDEPRHGYAIITRVGELSQQRVALSTGTLYQALDRLARAELVEVVRDEVVNGRARRHYALTNDGHAALRAEAVRMAEAAKVVLRVRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W151</accession>
    <name>U5W151_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glutathione import ATP-binding protein gsiA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_22360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363115.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22360"/>
    <dbReference type="EnsemblBacteria" id="AGZ42742">
        <property type="protein sequence ID" value="AGZ42742"/>
        <property type="gene ID" value="AFR_22360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4533"/>
    <dbReference type="eggNOG" id="COG4172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_11"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="278"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="310"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="518" mass="55151" checksum="050B4B12DE1F1818" modified="2014-01-22" version="1">MNPLVRVEDLHVTFDTGRRRIEAVRGISFTVERGECLAIVGESGSGKSVTARTLVGLAGPGAEVRAAALELDGHDARGFTERDWRKVRGGFAGLVLQDALVSLDPLRRVGDEIGEVLRVHRGSKKVEQLLTEVHVPEPARRARQYPHQLSGGLRQRALIASAVAGEPPLLIADEPTTALDVTVQAQILALLAERKAAGETLLLISHDLAVVSQVADRVIVLKDGRVRESGTTSLLLSGPRDPYTKKLLAAIPSAASRGRRLAGPGEVPVVRPEGGVVAEATALRKAYGRITVVDDVSLTIGRGEIVGLVGESGSGKTTVAQLLFGLVAPTSGSVTFEGAPWSGVAEGRRRPVRRKLQFISQDPLSSFDPRWTVARIVGEALPRGADVTPLLDRVGLAADVQDRYPRQLSGGQRQRVAIARALAPRPSLIICDEPVSALDVSVQAQVLDLLADIRATDGTALLFISHDLGVVHHLADRVLVMHDGKVVEQGPVESVFARPGHEYTRALLAAVPTLEVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W156</accession>
    <name>U5W156_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363390.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23045"/>
    <dbReference type="EnsemblBacteria" id="AGZ42879">
        <property type="protein sequence ID" value="AGZ42879"/>
        <property type="gene ID" value="AFR_23045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4670"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_10_4_11"/>
    <dbReference type="OrthoDB" id="1900922at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="21857" checksum="1C9004A718DBD81A" modified="2014-01-22" version="1">MDMKLEVVVVPVSDVDRARDFYTGLGWRLDAEFAAGDGFRVVQVTPPGSPASVIFGTSVTAQAPGSAQGLHLIVNDIDAAYDELKRHGAEPGEVFHDAGGVFHHAGTDARVPGPHPEHASYGSFLSFSDPDGNGWILQEVTTRLPGRVDPAATTFASTADLAAGLRRAAAAHGEHEKRIGAEDPDWPDWYADYLVREQAGTELPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1L6</accession>
    <name>U5W1L6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363683.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43093">
        <property type="protein sequence ID" value="AGZ43093"/>
        <property type="gene ID" value="AFR_24125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4887"/>
    <dbReference type="eggNOG" id="COG3153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121314_0_0_11"/>
    <dbReference type="OrthoDB" id="1367842at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="21778" checksum="1E42311F79B18F85" modified="2014-01-22" version="1">MVIKIVEQINDDETLEAAWELYLGAFRELNTMAVQRHLMYRHEFDEVMSDQRVQKHLCVDDDGTLCGLSTYTNDLTAVPLIAPEYFERHWPEHYAANKIWYIGFVALHPQAQGRRMFRQMVEQLYIIAATQGGLVGIDICSYNDEVLNFSHIFRSMVERLMPNMRFQRIDQQSYWLYDFSDEPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1L8</accession>
    <name>U5W1L8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362095.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17665"/>
    <dbReference type="EnsemblBacteria" id="AGZ41811">
        <property type="protein sequence ID" value="AGZ41811"/>
        <property type="gene ID" value="AFR_17665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3581"/>
    <dbReference type="HOGENOM" id="CLU_2662785_0_0_11"/>
    <dbReference type="OrthoDB" id="2192354at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="75" mass="8198" checksum="3D27B3BC3F138820" modified="2014-01-22" version="1">MPTFDLATLPATLVRPLVVAIVYLPIVLVTLFACLVLLVAIVLPATHGDFALKTLSLLRKWSADALRDTRVARRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Q2</accession>
    <name>U5W1Q2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23435"/>
    <dbReference type="EnsemblBacteria" id="AGZ42957">
        <property type="protein sequence ID" value="AGZ42957"/>
        <property type="gene ID" value="AFR_23435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4749"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074977_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="25"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="287" mass="31304" checksum="0A9199F9DA083BCB" modified="2014-01-22" version="1">MEQVRRLVAGQFPQWAGLAVRPVDNGGWDNFTFHLGSDMSVRLPSASEYALAVDKEHRWLPELAPQLPLPVPVPLAKGEPGEGYPFSWSVYPWLDGEPATFERVLDPGGFALELADFLVALQRVDPAGGPRPGLHNWFRGGTLRTYEGLAEQALAALGGRVDTELARQVWKAALDARWDGVERWFHGDIAPGNLLLADGALTAVIDFGTCGVGDPACDLAIAWTLLTRDGREAFRERLSVDDATWARGRGWALWKTLTICASDQPPADAMRVLGEVLSQTDGPSRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Q6</accession>
    <name>U5W1Q6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)-bd_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363550.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23460"/>
    <dbReference type="EnsemblBacteria" id="AGZ42962">
        <property type="protein sequence ID" value="AGZ42962"/>
        <property type="gene ID" value="AFR_23460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4754"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_5_2_11"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25599" checksum="D6CAF01FAD0E4C28" modified="2014-01-22" version="1">MKIAVMGGTGLIGSQVVKILNADGHEAVPHSPSSGVDLISGAGLPEALKGADVVVNLTNSPTFDDASPVFFRESMENLLGAAADAGVGHAVVLSIVGVDEVPDLVYYRAKVLQEELLAAGPVPYSIVRVTQFFEFMKSVLTWTADEKTVRLPATRVQPMASAEVAEAVAEVSTGSPLQGIRSYAGPEVFTLDELGKLALAAQGDTRTVVTDNTAGMFAAARGDALIAKADTIITKLTYREWLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1X3</accession>
    <name>U5W1X3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphoserine phosphatase rsbP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24785</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24785"/>
    <dbReference type="EnsemblBacteria" id="AGZ43223">
        <property type="protein sequence ID" value="AGZ43223"/>
        <property type="gene ID" value="AFR_24785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5021"/>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_43_9_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ANTAR" evidence="3">
        <location>
            <begin position="11"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="298"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="4">
        <location>
            <begin position="391"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="618" mass="67775" checksum="9A44E815430ACCAF" modified="2014-01-22" version="1">MGGGQQAYLEAMESLRSAARRPSTEAGWATVDRAVGLLAGRTHCRLSEAHRHLLKMAADQNREVVDVAAGVISMLDVPEPGEDHGLPELRPAVLPALGGSPVRVVEPWLAVVQAVLDLLPGQAALLTPLRDRSGGLADFVYAATSPEAVAPDGRRGRQLVGTRLGEQFPETVISDRWEAYRRVLETGEPTVVGPVPFGEAVYTVRCHRLGSSLFLTWVQHGREQGDNDRIAVTERLGNLGWGEWDLVRGEVHWSAQLYRIYERDPALGPLRREESEALEVAEDLPLRLAAAEAFERGEQVDVTARIKVNGRIKYLRTVADAVRDGAGRPLRIFGIVQDVTARETSVRRLAEVEEQLDEHRRSLHAEHELAARLQQIILPIPEGPIELPGVKVAVRYLPAGQESLVGGDWYHAAALRDGSVLFAIGDVAGHGTQAATTMAQLRYSLRALAVTTSDPANLLGHLNRLTCDLDQERPETAATAIVARYEPAQRRLTWAQAGHLPPLLSRAGRTEPLARPSGPMLGVVEDARYECATLRMELGDVLMLYTDGLVEHRRRTLDEGLDAVIRTVDEAVAASPRQPLAELLSRLRQANPDDDTCILAFRPVTGETQDLRRYLRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2J6</accession>
    <name>U5W2J6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Purine phosphorylase family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363973.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25450"/>
    <dbReference type="EnsemblBacteria" id="AGZ43354">
        <property type="protein sequence ID" value="AGZ43354"/>
        <property type="gene ID" value="AFR_25450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5156"/>
    <dbReference type="eggNOG" id="COG0775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060897_1_0_11"/>
    <dbReference type="OrthoDB" id="1860206at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="1">
        <location>
            <begin position="5"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="384" mass="41089" checksum="7DEC69B4C4B289B0" modified="2014-01-22" version="1">MKSRPIVVLTALNLEYQAVREHLTEIEVHRHQAGTRFEVGRLGVGGCRVALGLTGKGNHHSAVLAERAMAEFDPPAVLFVGIAGALWPEMSLGDVVVATQVYAYHGGTSEDDGLKARPRAWEIPHECDQIAHHIARTDTWARGLPEGERTPKVLFGAIAAGEVVLDSAVSAHARWIKQTYNDALAVEMEAAGLAQAAHLNRSLPAVVVRGISDRADGTKNDTDAEDWQPAAAANAALFAIALAEELAQTERHRERTRKDGNRTMSETVRNIAKGSAQVGVQAGTVHGGISMVQQPRSVAPEIPVQLAELREQISKAQAAGEVDRETFVAAEQELDTITELLPAPGEGAGSRLMLALRKLRGLLLDWTDLASKVTAIIAALRGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W339</accession>
    <name>U5W339_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364128.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25840"/>
    <dbReference type="EnsemblBacteria" id="AGZ43432">
        <property type="protein sequence ID" value="AGZ43432"/>
        <property type="gene ID" value="AFR_25840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5238"/>
    <dbReference type="eggNOG" id="COG1470">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830621_0_0_11"/>
    <dbReference type="OrthoDB" id="16179at28056"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="334" mass="33421" checksum="C657AEC3DCD90ACA" modified="2014-01-22" version="1">MELTLPATPVVLPPGVAVRVPVELHNPGPGARDVRLVVARGRAAGWAAAEPPATTVAAGAAITVELVLQTPHDQPPSASLVPFTVRAEDLGTGATAGFASGLLTVAVPVPVRGELVPRPGAAQTFDLHLGNDGAQPADVRVSAELDPPSGTTEIEPATAQVEPGGSLPVSVRARPARSLIGTPKKYAVIVTVHGPGPRPLLSAVGTGVVKPLVPKWVAAVVAVVLALGATAGVLLPRLSDDSAAPAVGRPFALVEAVPHTGGDGGRAAADARLATLTAAGMPVRLVDSLTSDDLDDNGGAGSWLLLHDGFGSADAATSFCTQWQAVAPGCKVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W341</accession>
    <name>U5W341_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_26780</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364493.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26780"/>
    <dbReference type="EnsemblBacteria" id="AGZ43618">
        <property type="protein sequence ID" value="AGZ43618"/>
        <property type="gene ID" value="AFR_26780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5429"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="27"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="341"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="375"/>
            <end position="382"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ43618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="581" mass="60795" checksum="EB42C43E96DB4E78" modified="2014-01-22" version="1">MAERRVQRRVGLPALLPYLREHRGTLIVVGSLSLVTAVAALVQPVLIRTVLDGISAGTSIGGAVALLVALLLTGAAIDGFRDFLLQRTAEGLVLTTRRRLARHLLRLPIAEYDTRRTGDLLSRVGADTTLLRAVVTSGLFEIVTGAVMVVGATIAMALLDPLLLGVAAAGLAVGLTVAITVSRRVRGLSEQAQARLGEMTSAVERSLSAARTIRASGAEDREAETVGRSATEAYDAGMRVARLRALIGPASTAATQGAFLLVLGVGGARVAAGAISVGDLVAFILFLFFLVLPLGQALNAYTQLQTGLGALQRIEEILDLPSETAEDRPAQAGSGSAEPAIVFENVTFGYPGGGTPVLQDVSFTVPAGSRVALVGPSGAGKSTVLALIERFYEVTSGTIRVGGTDIRDLPRDALRRRLGYVEQEAPVLAGTLRDNLLLTAPGADEARLLRALDEVNLGALATRTPDGLDVQVGEGGVLLSGGERQRLAIARTLLADAPILLLDEPTSNLDARNEAALREAIAAASFQRTLVVVAHRLSTVVDSDQIVVVEAGRVVATGPHRELLETSPLYRELATHQLLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5W354</accession>
    <name>U5W354_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26385"/>
    <dbReference type="EnsemblBacteria" id="AGZ43539">
        <property type="protein sequence ID" value="AGZ43539"/>
        <property type="gene ID" value="AFR_26385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5348"/>
    <dbReference type="HOGENOM" id="CLU_3131280_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="49" mass="5820" checksum="CD1E7448F5800093" modified="2014-01-22" version="1">MWGRVERRREKVRSQVEHARKGNHKIPTWLMAAVLGLVLLGWLYLIFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W379</accession>
    <name>U5W379_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20595"/>
    <dbReference type="EnsemblBacteria" id="AGZ42391">
        <property type="protein sequence ID" value="AGZ42391"/>
        <property type="gene ID" value="AFR_20595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4176"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074337_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="27945" checksum="07E020504FF68D08" modified="2014-01-22" version="1">MVISYFERTGEHRFRATEATGGAWAVDEQHISPMTGLLVHAVEQARDPADGMVVTRISMDILGVLKLEEFDVHVEVVRPGRTIELVEAVAIARGRPAVRARMWRMACPDTSAVAGGQPAALPGPETVEPWRLSELWQGGYIASVEVRPVGTPQPGRTTAWISSRADLIAGEKVSELASFVALVDTANGIAVRQSPADWLFPNLDLTIHLHRQPSGPWVGLDTSVVFGVSGQGLTSTVLHDVDGPVGRAEQILTVRPAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3S9</accession>
    <name>U5W3S9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560215.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28085"/>
    <dbReference type="EnsemblBacteria" id="AGZ43878">
        <property type="protein sequence ID" value="AGZ43878"/>
        <property type="gene ID" value="AFR_28085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5693"/>
    <dbReference type="HOGENOM" id="CLU_2911998_0_0_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="61" mass="6339" checksum="0CB9DC748334EC81" modified="2014-01-22" version="1">MSINDAGRQLAAEVAARFAERIATLAADLSAAERRVLSQLASRIVAADADRSGIDRATTPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W475</accession>
    <name>U5W475_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative FAD-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27805"/>
    <dbReference type="EnsemblBacteria" id="AGZ43822">
        <property type="protein sequence ID" value="AGZ43822"/>
        <property type="gene ID" value="AFR_27805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5635"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="2"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="404" mass="43170" checksum="51D4D58EAF0DA46D" modified="2014-01-22" version="1">MDVAIVGGGYTGLWTAYYLARADPGLRIAVLEAEYAGFGASGRNGGWCSGLFPVPVGKLARRYGREQAVAMHRALAASVDEVGRTGIDCSYAKGGTIALARSAPQLRRAEAEAREAEEYGLDVRLLDAAAASEICGATGVLGATYSPDCASLQPASLVRGLAAAVAASGVAIYENTRALRLSRGSVVTDHGTVRADVVVRALEGYTAGLAGHRRTLAPVYSLMIATEPLPQSTWDRIGLARRETFTDHRHMIVYGQRTADDRLAFGGRGAPYHFGSSVRPAYDQSPRVFSALRRTLTELFGIDPPIAYRWGGPLGIPRDWMPSVGLDNGLAWAGGYVGDGVAATNLAGRTLADLITGNLTDLTTLPWVGHRSRPWEPEPVRWLGINAALRGAGLRDRWELARER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4A6</accession>
    <name>U5W4A6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molecular chaperone GroES</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28540"/>
    <dbReference type="EnsemblBacteria" id="AGZ43969">
        <property type="protein sequence ID" value="AGZ43969"/>
        <property type="gene ID" value="AFR_28540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5786"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="10"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="31910" checksum="83D87B01133C3A28" modified="2014-01-22" version="1">MKRLQYDRYGGPEVMRLAEFEPPRPGPGEVLVRVRAAASNALDWKMRNGEMKLLTGRSFPRAMGHDFAGVVEAVGAGVTRLQAGDAVLGGARFRQAGAFAEMVTVPESAVVLKPVDLSYEQAAALPTVGVTAYQATAGIRPGQAVFVNGCLGGVGRAAVHFARARGASVAGSCRDTTTDEARELGVDPVVGFGFAPAALAERFDLVFDTPGMLPLATARTLLKPGGAIIDIVPTPAKMIRSVLPGPFRTMTGRPVTADLEEVARTLRLPIARTVGLAEAIPALTELERDQRPKGGKLVITMAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4M2</accession>
    <name>U5W4M2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560490.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29460"/>
    <dbReference type="EnsemblBacteria" id="AGZ44153">
        <property type="protein sequence ID" value="AGZ44153"/>
        <property type="gene ID" value="AFR_29460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5971"/>
    <dbReference type="eggNOG" id="COG5485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100997_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436">
        <property type="entry name" value="PTHR38436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07366">
        <property type="entry name" value="SnoaL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="146" mass="15911" checksum="44E7396ED1A17C0F" modified="2014-01-22" version="1">MSPSEQLESNKQIARDYIEQVFNKHNPLAARDFVTDDVVWHGGALGDLTGAENLTGLLTGFLGSLPDLYAAEQDVVAENDLVMVRLVVTATVKGQLLGVPADGRTVRWDAVDIYRVTDGRISEEWAADDLVTIMTQLGAFTPPWAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5A5</accession>
    <name>U5W5A5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bcr/CflA family efflux transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_30690</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Bcr/CmlA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30690"/>
    <dbReference type="EnsemblBacteria" id="AGZ44398">
        <property type="protein sequence ID" value="AGZ44398"/>
        <property type="gene ID" value="AFR_30690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6214"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_47_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004812">
        <property type="entry name" value="Efflux_drug-R_Bcr/CmlA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00710">
        <property type="entry name" value="efflux_Bcr_CflA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="21"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="403" mass="41028" checksum="9AF5F4CFD0A60F22" modified="2014-01-22" version="1">MSTQPTAPATDTDPAPRGPLLMVMLGLITAIGPLSLDMYLPALPAIAGDFDVPAAQIQLSLTTCLIGLALGQLVCGPLSDRWGRRRLVIIGVGGYAVFSLLCALAPNAPALAGLRLLQGFAGGAGIVVARAIVRDLHSGRAAAKYFSRLTLIVGVAPIAAPSLGGAVLTVTSWPGIFVALGLIALLVTLLIAVRLPETLPPQRRSTGGLRATARAARGLFQDRIFLGYALAQAFAFAALFGYISGSSFVLQDGYGLSPTTFGVIFGLNACGLVVLSQANGKLLDRFPPRRLLLTSLSTQAIAGVTVLVAAVAGSLPVLIAGLFLLVGAIGMVMPNSTALALDRHPERAGTAAALLGGLQSVVAALAAPLVGFGDPGLGLPMALAILSFALVALLSVLVLARRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5F1</accession>
    <name>U5W5F1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative LysR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363731.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24375"/>
    <dbReference type="EnsemblBacteria" id="AGZ43141">
        <property type="protein sequence ID" value="AGZ43141"/>
        <property type="gene ID" value="AFR_24375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4938"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_11"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="33030" checksum="8CF762BE17EBBA08" modified="2014-01-22" version="1">MGQIDLRRLRLLRELRDRGTVTAVGTALHMTSSAVSQQLALLAKDVGAPLLEPHGRRVRLTDAAWLLLRHADLLFAQLEVAQTELDAYVSGAAATVRMDCFATAVGALAVPAMRRLGEDAPRLTVRIREADTEEALDRLLRDETDVVVGLDGEVRVDDARFTRTPLLVDPLDLVLPSGHPRAGADELRLADLAGEEWIVPPAGLCRDIAVRECSAAGFTPRTAHTADSYVSVFALVRAGYGVALVPRMAAPDREPGLTVRAPRAQQPSRQVSLFVRQAGDQAPHMRLVAEALCATADELEKAFSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5F2</accession>
    <name>U5W5F2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable primosomal protein N'</fullName>
            <ecNumber evidence="9">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-dependent helicase PriA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">priA</name>
        <name evidence="12" type="ORF">AFR_30570</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the primosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the helicase family. PriA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30570"/>
    <dbReference type="EnsemblBacteria" id="AGZ44374">
        <property type="protein sequence ID" value="AGZ44374"/>
        <property type="gene ID" value="AFR_30570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6189"/>
    <dbReference type="eggNOG" id="COG1198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015485_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00983">
        <property type="entry name" value="PriA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005259">
        <property type="entry name" value="PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041222">
        <property type="entry name" value="PriA_3primeBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042115">
        <property type="entry name" value="PriA_3primeBD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17764">
        <property type="entry name" value="PriA_3primeBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PriA_3primeBD" evidence="11">
        <location>
            <begin position="24"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="567"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="128"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ44374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="834" mass="86370" checksum="3066138BB1D8E25B" modified="2014-01-22" version="1">MKAAKAAKPKPDREPAARLPIARVSVEVPLAHLDRPFDYLVSVADDEAAQPGVRVKVRFAGQLVNGFLLERVESSPHGGKLAYLDKVVSPERILDPEVARLARAVADRYAGNLSDVLRLAVPPRHARVESQAQPAVASPASVVTAPQDDPGEPGAISSPGSASDVADPGDEPSRVSVPAADREGPTSVKLGLREPGDGVEDAPVNGLPPPVSATNGGVPGELSGAGADFSGLDNSTEGAPDASGGEDLCRATEDSERQERSGQPSPVGVSAPPSGTEADPATGELAEPDAGLGAAAGAGSLEGRSAEPSPNAGSAETAVQHGWGIYPTGDAFLHALGEGRAARAVWSALPGEDWPARIAEAAGATVRGGRGVVIVVADARDLERVDRALSKVLGDGRHVALNAAAGPAERYRRFLAASRHRVSVVAGTRAAMFAPVADLGLVVIWDDGDDLHAEPRAPYPHARDVLLTRAQLAGCAALVAGFARTGEAQLLLETGWAKEIVANRDTLRARSPLIAPTGDDPQLARDPGAVTARLPSLAWQAARGALQAGAPVLVQVPRRGYLPSTACAECRTPARCPHCSGPLGLAGSRDVPACHWCGRAAADYACPACGGRRLRASITGARRTAEELGRAFPGVPVRTSGRDEILDTVPGEASLIVATPGAEPVAEGGYGAVLLLDTWALLSRANLRAAEETMRRWLSAAALAKPGPAGGRVVVVADGSLAPVQALLRWDPGWFAARELAERRELGFPPAARMASLTGTAEAVAEFLDAARLPEEVEVLGPVPAQEEQERMLLRVSRGRAAELARLLHEAAAVRSVKKSALPVRIQIDPAELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5N9</accession>
    <name>U5W5N9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_30310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30310"/>
    <dbReference type="EnsemblBacteria" id="AGZ44322">
        <property type="protein sequence ID" value="AGZ44322"/>
        <property type="gene ID" value="AFR_30310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6137"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="198"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="394" mass="41365" checksum="2133EC429EF4F83A" modified="2014-01-22" version="1">MDLGPPGEDPAYGRDVNRARVSDAAAVVLSAAIGVLMVRSSAFPAPGGSWMLVAQVALSVCLFARRRFPVALAWLMVAAAAVIAVAGSGPLTPVTTDSNAMPWLPAAAPFAAYSATAHAGKRWAGLVPAGVLVVIVSHGWDSPPDSPWTLQSLLFIGGPALLGLYVAARRRLLRSLLDRTERAEREQRLLAGRARAEERSRIAAEMHDLVTHRVSLMVLQAGALQVTATDAATRTTAEDLRTTGCQTLEELRDLLRALRFDPEAPVADLARLVAESGVDAALVEDGDPAQPSPVVARTAYRIVQEALTNVRKHAPGARVTVTLRYLPERVRLSVHSTAPAGGPDPRLAASGSGNGLLGLRERVELVDGTLSAGPDGTGGFRVEAELPVTVGGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Q1</accession>
    <name>U5W5Q1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribonuclease bn</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560861.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31340"/>
    <dbReference type="EnsemblBacteria" id="AGZ44528">
        <property type="protein sequence ID" value="AGZ44528"/>
        <property type="gene ID" value="AFR_31340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6343"/>
    <dbReference type="eggNOG" id="COG1295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045539_3_1_11"/>
    <dbReference type="OrthoDB" id="1421045at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017039">
        <property type="entry name" value="Virul_fac_BrkB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30213">
        <property type="entry name" value="PTHR30213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03631">
        <property type="entry name" value="Virul_fac_BrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035875">
        <property type="entry name" value="RNase_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00765">
        <property type="entry name" value="yihY_not_rbn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="34099" checksum="DB92AB59B8B6B10E" modified="2014-01-22" version="1">MTAATTAVPSRVRQLHWSTWRGVLWRSGKGFLDDDCGDLAAALTYNAVLAIFPVGIVVVALVNLVTDGRTAVDTVIGILKDLGAGSVVADENLTSVLDAIIVQQSSAKVLLSFGLLGALWSASNFVGVFTRASNRVYGVREGRPFWKLRPLQILVSAVALLLMAAVAAGLVISGPLVDAVGNALHAGDTARLAWSIGRWPVLVLILMLLLSLLFWIAPNVKQPRFRWITLGGFVALLVWALASFGFGLYVANFASYDKTYGSLGAVIAFLVWMYLANSALMLGVEINAEVQRGRQLQAGEDPDPPLPVREEPDDDQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5W6</accession>
    <name>U5W5W6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_25350</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363939.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25350"/>
    <dbReference type="EnsemblBacteria" id="AGZ43336">
        <property type="protein sequence ID" value="AGZ43336"/>
        <property type="gene ID" value="AFR_25350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5137"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="12"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ43336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="504" mass="54981" checksum="EF99D8D23CB1E208" modified="2014-01-22" version="1">MTVSPVVVNSRYRLGRTLGEGGMGRVWLARDEVLGRDVAVKEIIPPDDLVETEHAKVQERSLREARAAARLSHPNVARVFDVFEADGHTWIVMEYIPSRTLAEAIRDDGPLPPRRVAEIGLEVLAALEASHQAGVRHRDVKPANILLGDDGRVVLTDFGIAAIEGDGIVTSADMVVGSPQFMSPERLRDGTAALGSDLWSLGASLYTAVEGHSPYERKSTVETLTAVAVDEPDPAHQPGPLAPVLEGLLRKDPAQRIGLPETRRLLRRAASVSPVRRPGRKVALAAAVVVAVMLAAGTAVWLGRRTETTTASPPPAAVATTAPVVPSPSALSPSPTPPSRTPESTVTTTVAAGNTRPPLPNGWRDYRDPTGFRLYVPKGWTRSKEGSIVYFRDPDSGTTLGIDQTNKPRPNPVADWQGKAEYRVGRGEFPGYREIKIKAVKYWQKAADWEYTFNGSTRQHVNNRGVITSKKQAYGIYWQTSDSRWKAQRPKLDLIFASFRPDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6C5</accession>
    <name>U5W6C5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphoglycerate dehydrogenase-like oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_32155</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32155"/>
    <dbReference type="EnsemblBacteria" id="AGZ44689">
        <property type="protein sequence ID" value="AGZ44689"/>
        <property type="gene ID" value="AFR_32155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6510"/>
    <dbReference type="eggNOG" id="COG1052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="4">
        <location>
            <begin position="34"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="5">
        <location>
            <begin position="123"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="34855" checksum="A2914DA66113A3A6" modified="2014-01-22" version="1">MLRAYREGMKIVVLDDYQGVARELGPFDTLTGDQVEVLREHVDDQDELVEALRGAEVVVAMRERTAFPAGLFDRLPDLRLLVTTGMLNAAIDLAAAAEHGVTVSGTVMSGSAKSSNTAELTWALILACRRHVVAEDRALRNGRWQTTLGTDLAGSTLAVLGLGRLGTQVARIGQAFDTRVIAWSHNLTPERAAEAGATWVPREQLFADADVLTVHLKLSERSTGLIGAPELAAMKTTAILVNTSRGPIVDETALVAALTTGSIAGAGLDVYDTEPLPAGNPLRGAPNTVLLPHLGYVTEAGYRTMFQQVVEDIAAWRSGSPVRVLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6F7</accession>
    <name>U5W6F7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative proteasome component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Pup ligase/Pup deamidase family. Pup deamidase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364350.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26415"/>
    <dbReference type="EnsemblBacteria" id="AGZ43545">
        <property type="protein sequence ID" value="AGZ43545"/>
        <property type="gene ID" value="AFR_26415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5354"/>
    <dbReference type="eggNOG" id="COG4122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040524_1_0_11"/>
    <dbReference type="OrthoDB" id="537546at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022366">
        <property type="entry name" value="Pup_deamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004347">
        <property type="entry name" value="Pup_ligase/deamidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42307">
        <property type="entry name" value="PTHR42307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03136">
        <property type="entry name" value="Pup_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018077">
        <property type="entry name" value="UCP018077"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03688">
        <property type="entry name" value="pupylate_PafA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR018077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="505" mass="56763" checksum="99388AECD6BD63FA" modified="2014-01-22" version="1">MSVRRIMGTEVEYGISVPGQPGANPMVTSSQVVNAYGARPELNRGGRARWDYEEESPLRDARGFTYSGAAYDPADALADEDLGLANVILTNGARLYVDHAHPEYSTPECTNPMDIVLWDKAGERVMAEASRRAATIPGTHRIQLYKNNTDNKGASYGSHENYLMSRQTPFADIVAHLTPFFVTRQIFCGAGRVGIGQDGSGAGFQISSRADFFEVEVGLETTLKRPIINTRDEPHSDADKYRRLHVIIGDANLSEISTYLKVGTTALILNMIEEKVFTGELGVADPVSELKAVSHDPSLKHLMRLRDGRRLTALDLQWAYYERAKEFCDERYGTDTDEQTRDVLQRWEDVLDQLGRDPMQLSDQLDWVAKLRLLEGYRERENLTWSSPKLGLVDLQYSDVRPEKGLYHRLVARGSMKTLLDDEQTQRAMVKPPEDTRAYFRGECLARYASEVVAASWDSVIFDVGRESLVRVPMMEPERGTKKHVGALFDKCDTAKDLLEVITSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6G2</accession>
    <name>U5W6G2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc metalloprotease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_26440</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26440"/>
    <dbReference type="EnsemblBacteria" id="AGZ43550">
        <property type="protein sequence ID" value="AGZ43550"/>
        <property type="gene ID" value="AFR_26440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5359"/>
    <dbReference type="eggNOG" id="COG1994">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037123_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016483">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006404">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="11">
        <location>
            <begin position="65"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006404-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006404-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ43550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="387" mass="40598" checksum="8747C4B1E038B79A" modified="2014-01-22" version="1">MDEGARQTRPARRPGRLVGHVLGIPVFLHPSMLLLALLVTVVYGGYVQSELGLPGPFAYAVGFGFVVCLLGSVLLHELGHAVTAKRFGIGVRGITLELLGGWTEMDRDAPTPRIDALVSLAGPAVSFALGGLATAAAFALPDRTVAGEIAFQLAASNVIVAIFNVLPGLPLDGGRALRAGLWALIKDRNRATEAAAWAGRGIALLTGAAVFTLYRLNVITLFGLIFILLVVFTLWQGAGQSIRLARMTRRFPLVDLSALARPLYAVPSGTPLAEAQRRADAEGRAKDTLAVADASGRVVALVDTAGAAAVPADRRPWVSVDTVARDLGRLPALALGLSGEQVVRAVQAHPGAQYVVTSGEDVVGVLHVADLAELLEPRRTARRPAPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W717</accession>
    <name>U5W717_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-1,2-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33675"/>
    <dbReference type="EnsemblBacteria" id="AGZ44993">
        <property type="protein sequence ID" value="AGZ44993"/>
        <property type="gene ID" value="AFR_33675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6816"/>
    <dbReference type="eggNOG" id="COG3537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003690_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005887">
        <property type="entry name" value="Alpha_mannosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041371">
        <property type="entry name" value="GH92_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012939">
        <property type="entry name" value="Glyco_hydro_92"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07971">
        <property type="entry name" value="Glyco_hydro_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17678">
        <property type="entry name" value="Glyco_hydro_92N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01180">
        <property type="entry name" value="aman2_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="49"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1115"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1410" mass="150191" checksum="6DBAE926848D3929" modified="2014-01-22" version="1">MIATAASVATGGSALAAAAESFTSSFETGEPQPTWTDTVDADAGSEGVDGTVVVGMPGSLRSHVTAIAVNAQPNSSEGANNLNDGDSATKWLVDTPTSWAQYTLDAPAEVIKYALTSANDSPERDPRDWTLQGSTDGETWTTVDTQTGQTFTERFQTKTYTVADPASFPIYRLNITGHPSGNLTQLAELELADANVTAPPTGPMQSRLGNGPASSPTAKAGVGYTGLKAFQIAGRHTAEGRGYSNNKVFDVDVAVTDRTELSYLVFPEFNREDLSNPATYTAVDLAFTDGTYLSDLKALDQNGIVVSAPAQGASKTLYTAQWNKRTSLIGSVAAGKTIDRILVSYDKPSGPTTFRSWFDDISIAEVAPVKPKAHLSEYAETRRGTQSSGDFSRGNNFPATAVPHGFNFWTPVTNAGTTSWLYEYHRANNAANRPTLQAFSTSHEPSPWMGDRQTFQVMPSAAAGVPDANRANRALAFGHENEVAKPYYYGVTFDNGLKTEFAPTDHAALFQFTFTGDSSNLILDNVNNNAGLTIDAANNAVSGWSDVNSGGLSAGFTRMFMYATVDAPIASSGSLASGNRPSTGYLKFDTTTNKTVQMRIATSLISVAQAKKNLDLEIAADATLASVRDKAQSLWDAKMHTVEVEGASEDQLTTLYSNLYRLFLYPNSAYENTGTNEAPVYRHTVQSAVTSPGSTPTETGAPIKDGKVYVNNGFWDTYRTTWPAYSLLTPKEAGEMVDGFVQQYRDGGWISRWSSPGYANLMVGTSSDVAFADAYLKGVPGIDAETAYLAALKNATVTPPNQNVGRKGLSSSIFKGYTPSDATGEAMSWAMDGYINDFGIANMAEALAKKGGPKAKDYAEQAEYFRNRALNYVNMFDPSVDFFQGKSTAGVWRNPPDTYDPRVWGYDYTETNGWNMAFHVPQDGQGLANLYGGKDGLAKKLDKFFDTPETATFYGSYGGPIHEMYEARDVRMGQWGFSNQPSHHIPYMYDYAGQPYKTQKLVREAMSRLYLGSEIGQGYPGDEDNGEMSAWQIFSALGFYPLQMGSPEYAIGSPLFTKATVNLDNGKKIVVNAKNNSRSNVYVQSLKVDGKAYNSTALPHSVIANGATLDFSMGSEPSSWGTGDDAAPPSITKGSAVPTPLKDLTGPGRGTSSEPALVDDTSTTAATFATATPSWQYQFTSAGEQADYYTLTSGAVAGDAKAWKLKGSYDGTTWTTIDERSDQTFDWRLQTRPFKITSPGRYAYYRLDVSANTGEASTTLAEVELLGIPAPACTTTISDKVAGAVSVRSGVTCIKAGAQVTGLISVSKGASLYATGANLRAAVTATGAGTVSLLNTTVAGPVTVTGSGPVSLENSTLKSAVTLLTNKKSTVVADNTITGPLTCSGNKPAPVNNGLTNAGSGVRLGQCGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W770</accession>
    <name>U5W770_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560172.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27870"/>
    <dbReference type="EnsemblBacteria" id="AGZ43835">
        <property type="protein sequence ID" value="AGZ43835"/>
        <property type="gene ID" value="AFR_27870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5648"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_4_11"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="20239" checksum="34330859084122B4" modified="2014-01-22" version="1">MSALPFPEPPLTDGTVLLRAWHEADEAQRHAGFADPLCQQFSWSPATPFTEEHTRAAFAQDEEGRLAGESLNLAVTDATHTGVIWGGTSIYDVDPRSASASVGYWLAPQARGRGIATRTLRLLSAWALGQLAVERLTLTCAPDNAASQRVALRCGFVREGVLRSHLPFQGARRDTMIFSLLPGELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W786</accession>
    <name>U5W786_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33730"/>
    <dbReference type="EnsemblBacteria" id="AGZ45004">
        <property type="protein sequence ID" value="AGZ45004"/>
        <property type="gene ID" value="AFR_33730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6827"/>
    <dbReference type="HOGENOM" id="CLU_123724_0_0_11"/>
    <dbReference type="OrthoDB" id="2076339at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="18626" checksum="4DC0374CC0E74649" modified="2014-01-22" version="1">MTSAVIAALTSSFLAWQAIRVNQNMNHMPVVLEVLKPHRTPDFVRKETLLWKELSDHPAELGFFGLPEPIMSHAIEVGTYYQVLSYTSLYGIADGEFIAVQTHYRLLRTWEALREHVEGERVLRGGHNTFLNSYEEFAGLVATMDIDAATRRLVRRSGKLRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7E2</accession>
    <name>U5W7E2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560208.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28050"/>
    <dbReference type="EnsemblBacteria" id="AGZ43871">
        <property type="protein sequence ID" value="AGZ43871"/>
        <property type="gene ID" value="AFR_28050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5686"/>
    <dbReference type="eggNOG" id="ENOG5033ACW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056160_0_0_11"/>
    <dbReference type="OrthoDB" id="1692552at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="26685" checksum="7749DDB748229336" modified="2014-01-22" version="1">MNETPTPPAWIHWPIRVLAVIFVLPFRLLWELLVVIGRFLARYVGRPLAWLFHHAVVVPVAWVWHYVVVVPISWLWRHLVVVPLEWLAHRLLVVPGRWLWARRRWALRPFGLLVRYLLVIPVVWFFTVTEPLWTALGQALVWTFTMIGRGIDAVAVLAYRWVLHPGWIGAGWVLRQAYRWLLRPVGLAIAWVWRHTVVPAYRMISASGRWVREAVLRPVLVSVGLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7H5</accession>
    <name>U5W7H5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AraC family transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561475.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34400"/>
    <dbReference type="EnsemblBacteria" id="AGZ45138">
        <property type="protein sequence ID" value="AGZ45138"/>
        <property type="gene ID" value="AFR_34400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6960"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_100_0_11"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009594">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06719">
        <property type="entry name" value="AraC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="184"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31710" checksum="B4E299840F7751FC" modified="2014-01-22" version="1">MIEDLARVIAAHTAGFWTDTEVPRLGLVSADERPGAIDMLYTPMICFVGSGSKRTAAGDRNWQVDSGHMFLASLEMPITATFERMPYRSAVLQLDQQVLADLLLELGAAAGRESTDPGGMTTARMSPELIGAVVRWVGLLDTPEDIPALAGRIEGEILYRLLGSPLGPVLRQFALAESHVTQVRGAARWIHQHFAEPLSVEAIAAVAHMSTATLHRHFKSATGMSPLQFQKSLRLQEARRLLVAGDATAALVGEKVGYASATQFTREYRRAYGAPPAQDALRLRTLLKLLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7N3</accession>
    <name>U5W7N3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coagulation factor 5/8 type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33795</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33795"/>
    <dbReference type="EnsemblBacteria" id="AGZ45017">
        <property type="protein sequence ID" value="AGZ45017"/>
        <property type="gene ID" value="AFR_33795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6841"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021574_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="GH16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666414" evidence="1">
        <location>
            <begin position="28"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="5"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="166"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="3">
        <location>
            <begin position="307"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="567" mass="61050" checksum="911E087E26AE2FC0" modified="2014-01-22" version="1">MRVIALTTALLGLLAAYLVATVRTADAAEVVVSQGKPTTASSVEWAGTPASSATDGNTGTRWSSAFAANQWIQVDLGATTAVSSVKLNWEAAYATAFTVQFSTNGTTFTNATGTLRGNAGEQTIAVTGNTRFVRLNLTERALAIYGYSLWEFQVFAGSTTPPTDPTTPPPAGGATLLSYNKPGAASSEQNDVNCNPCTVPKAFDLDPATRWATSSTTGWVDPGWIYVDLGATAQISRVELQWDPAYATAYKIQVSPNASTWTDIYSTTTGKGFKEVLNVSGTGRYVRMYGTTRSSAYGYSLYDFNVYGTGGAPNTPPARPADPTFPATNLVWNDEFNDPAGTKPSTAKWTYDPGVPQNGEVQYYTENSNNAQMDGGGNLVIEARKEASNGREYTSARMNTGGKFTAQYGRVEARIKVPKGNGLWPAFWMMGNDFLTGRPWPYNGEIDIMEVLGRNTTEGYSTLHAPQYNGGGGYGQKYTTPGAADLSQGFHVWAAEWDSKGIRFLLDGTQVFYASKATVEATRGPWIFDHPFYIILNLAVGGDFPGPVDASTPFPARMLVDYVRVYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7N4</accession>
    <name>U5W7N4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561466.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34355"/>
    <dbReference type="EnsemblBacteria" id="AGZ45129">
        <property type="protein sequence ID" value="AGZ45129"/>
        <property type="gene ID" value="AFR_34355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6951"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_8_0_11"/>
    <dbReference type="OrthoDB" id="1442962at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="24412" checksum="C1F1F3E504EC590C" modified="2014-01-22" version="1">MTASIGPVKATPYLWPYDGSVPVERTALICIDWQTDFCGPGGYVESMGYDIALTRAGLPATAKLLAHVRSLGMLVIHTREGHDPDLSDLPANKRWRSAQIGAEIGSQGPCGRILTKGEPGWEIVPEVAPVAGEVIVDKPGKGAFYATNLDLVLRTHGITHLILTGITTDVCVHTTMREANDRGYECLILSDCTGATDPANHTAALHMVTMQGGVFGCVSTSDDVIAATEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W920</accession>
    <name>U5W920_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative isobutyryl-CoA mutase chain A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_37170</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562023.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37170"/>
    <dbReference type="EnsemblBacteria" id="AGZ45688">
        <property type="protein sequence ID" value="AGZ45688"/>
        <property type="gene ID" value="AFR_37170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7520"/>
    <dbReference type="eggNOG" id="COG1884">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_5_1_11"/>
    <dbReference type="OrthoDB" id="154460at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="METMALONYL_COA_MUTASE" evidence="3">
        <location>
            <begin position="28"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="557" mass="61430" checksum="5F79235D5E3D087D" modified="2014-01-22" version="1">MDAADIAAGRERWQQRYDAARKRDADFTTLSGSPVDPVYGPADGDTVEDFERIGWPGEFPYTRGLYPTGYRGRTWTIRQFAGFGNARQTNERYKMILAAGGGGLSVAFDMPTLMGRDSDDPQSLGEVGHCGVAIDTAADMEVLFDGIDLQQTTTSMTISGPAVPIFVMYLVAAERQGADLGKLDGTLQTDIFKEYIAQKEWLFEPEPHLRLIGDLMEYCAAEIPKYKPLSVSGYHIREAGSTAAQELAYTLADGFGYVELGLSRGLDVNTFAPGLSFFFDAHVDFFEEIAKFRAARRIWARHLRDDYGATSEKALWLKFHTQTAGVSLTAQQPVNNVVRTAVEALAAILGGTNSLHTNALDETLALPTDESAEIALRTQQVLMEETGVTNVADPLGGSWYVEALTDRIEAEAEEIFTRIRELGHDGSMTSGILRGIEDGWFTSHIAEAAFVYQQALEKNEKRIVGVNVHTGTVAKDLEILRVSHEVEVEQKRALGARKDNRDPVRVQQALTRLVDVSRTKENMIPAMLDAARAEATLGEICEALKGEWGIYREPARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9L2</accession>
    <name>U5W9L2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31680"/>
    <dbReference type="EnsemblBacteria" id="AGZ44596">
        <property type="protein sequence ID" value="AGZ44596"/>
        <property type="gene ID" value="AFR_31680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6412"/>
    <dbReference type="eggNOG" id="ENOG5033IBR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1282005_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="22837" checksum="F93A1AA9AEABB09D" modified="2014-01-22" version="1">MTTIPPYATMLGFTRYVARTGPAKATFVGGLRRQRERRSGFNPHGQLVKALKADIAFRTGGSYLNAVPDLVKPRWKPLYEAVSAGATTYLHSLGDPEDVGLAQTRDALASVGPLAVKINPHFGLRYADGTREAVRLHFDEQPPTPEAITATLHLMARHMDQILPNAEPVLVDLRRGTTHRIDPGAKTGDVERWLAGEAAAFTAMWTATAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9L9</accession>
    <name>U5W9L9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Lipid A export ATP-binding/permease protein msbA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_31730</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560939.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31730"/>
    <dbReference type="EnsemblBacteria" id="AGZ44606">
        <property type="protein sequence ID" value="AGZ44606"/>
        <property type="gene ID" value="AFR_31730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6423"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_9_11"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="34"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="347"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="380"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ44606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="586" mass="63963" checksum="77006D529207BAF1" modified="2014-01-22" version="1">MSRRAPRATIDPDVRKTWLRRALPIVKAHRGLLITSLVLSFAGLVVQVQIPNLVRIGIDKAIVDRAADLPVYVWWIVGLALLQGVINFLARLYLLRTAYELEYDLRNIVYAHLARMPFGFFDRVQSGELMSRSSSDIRAVQMYLSFGPSILVQCLIAVVAFGLMLSINVPLAIVAMATMPVIGFLGVGMRKAIFPVSWLIQARLARLATVVDENIQGVRIVKAFAAEGRQLRMLAEAADSIRWAYLQDARIRSRWNPVLDNLPRLGLALVLLVGGLMVIDGSTTVGTIVAFNSYVLMLQPPFRMLGMMIMMGQRASASARRIYEILDTPSDVVDPAAPVDAVLRGDVRFEGVRFAYPNGTPALDGLDLHVRPGETVAVVGATGSGKSTLGRLLARYYDVTGGRVVIDGHDVRTLRLAGLRDQVGIVPDEPFLFSLSLHDNIAYGRPDASRDDVIAAATAAGADDFVRDLPEGYDTVVGERGYTLSGGQRQRIAIARALLVNPPVLVLDDATSAIDVTVEQRIHRSLREQLRGRTTLIVAHRLSTIGLADRVVLVEGGRIVADGTHESLLATEPRYARVLASSEVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9M1</accession>
    <name>U5W9M1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562179.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37950"/>
    <dbReference type="EnsemblBacteria" id="AGZ45844">
        <property type="protein sequence ID" value="AGZ45844"/>
        <property type="gene ID" value="AFR_37950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7675"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_0_0_11"/>
    <dbReference type="OrthoDB" id="1381894at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="PlsC" evidence="2">
        <location>
            <begin position="81"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="26713" checksum="E2531B6249F5093F" modified="2014-01-22" version="1">MTSAVWQPTSACGPQCRPAGDHAVRAGTAVLRVTALIGVLISALVLVPLLRRTAARTVSRGILAALGVKLVWRGPAPRPGSLLVANHVSWLDVVALLAVTPVRLVAKHDVRAWPAVGALAALTGSIFVDRTRPKRLPATVAEVAAALRTGRSVAVFPEGTTFCGAERGRFRPAVFQAAVDAGAPIVPISIRYASPAAAFVGDDTLWSSVRRVAGLRGLTVTLVGSPALRPEPGADRRALARAAQASAGLRSAKFDLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAR6</accession>
    <name>U5WAR6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561373.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45036">
        <property type="protein sequence ID" value="AGZ45036"/>
        <property type="gene ID" value="AFR_33890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6860"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_932667_0_0_11"/>
    <dbReference type="OrthoDB" id="1967616at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="174"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="34626" checksum="34A94B9C6E694D63" modified="2014-01-22" version="1">MALTLVEMDDEDFARRRGPMVTGYAEAISTARGLSVPEAEAESERDIADRLPRGAATRGQLLRKAVVDDAEVGWIWISLPSSTMPEMAWISDIEVDTGHRTRGHGAAIITAVEAELVELGVPRLGLNVFGDNPTAARLYTRLGFEVTARQLARSLADVPAAPGIELVPMADYESRIETLFADYARDLVEEQGIWHGEAEERAGRRLAELLPHGRGTEGMILRTVLAKGEPVGWLWAAMPAPPRPGMGWLHNLDIDEPFRSRGYGAGAIAAVEAELVHRGVRSLGLNVHGANDRARKLYERLGYELLAQQMAKDLPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB10</accession>
    <name>U5WB10_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561497.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34510"/>
    <dbReference type="EnsemblBacteria" id="AGZ45160">
        <property type="protein sequence ID" value="AGZ45160"/>
        <property type="gene ID" value="AFR_34510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6981"/>
    <dbReference type="HOGENOM" id="CLU_119432_0_0_11"/>
    <dbReference type="OrthoDB" id="1400123at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="16075" checksum="C6CDAF0310F79E77" modified="2014-01-22" version="1">MISEVGGWPAGENVEDLARYAMDQGAEGYDIHEVRVCTCAACGQQVFGVQGDLEERAVQRICRGCGAEHFIADSGEHWDAMKPAIMVCECDGDSDEEDFNVAVGFSLYANKTGVRAIAVTGRCLACGRLGYWTDWMVRTGDLDLLDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB65</accession>
    <name>U5WB65_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosomal-protein-alanine N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39890"/>
    <dbReference type="EnsemblBacteria" id="AGZ46232">
        <property type="protein sequence ID" value="AGZ46232"/>
        <property type="gene ID" value="AFR_39890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8071"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_40_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="23710" checksum="E0B2AE528C14C068" modified="2014-01-22" version="1">MLGSTPGWPAVLADGPVLLRPYRRSDARAWAEVRIANQAWLSPWESSPPGPWAEMNSARAYLYVYRDMKRAARRGDSMPFAVCLRENGDERLVGHINLGSIVRRAFSSAYVGYWVDARVAGRGVIPTALALAVDHAFGAGGLHRVEINIRPENGPSRRVVEKLGFREEAYHPRYMHIDGAWRDHLGYAMTSEEVSAEGGLVARWRRLRADAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBZ4</accession>
    <name>U5WBZ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562844.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41290"/>
    <dbReference type="EnsemblBacteria" id="AGZ46512">
        <property type="protein sequence ID" value="AGZ46512"/>
        <property type="gene ID" value="AFR_41290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8356"/>
    <dbReference type="HOGENOM" id="CLU_1801922_0_0_11"/>
    <dbReference type="OrthoDB" id="2159129at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046065">
        <property type="entry name" value="DUF6023"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19487">
        <property type="entry name" value="DUF6023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16149" checksum="91C6A171077B4F6D" modified="2014-01-22" version="1">MDGDRTRGATLYGLAAVLLLAGVVWFVRAAPDRGDEVAVAAARETVERLLPDLPLQSGGETMVLPAGERAERTTGVRGGSYKLVMLCVGDGQVRVRLSSRGVDSGRAVPCAAAGEPSPVELTVGLASEFYLAVSAETDGPAVFRWRVTRSRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC49</accession>
    <name>U5WC49_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_36495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561891.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36495"/>
    <dbReference type="EnsemblBacteria" id="AGZ45555">
        <property type="protein sequence ID" value="AGZ45555"/>
        <property type="gene ID" value="AFR_36495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7384"/>
    <dbReference type="eggNOG" id="COG2340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048111_2_2_11"/>
    <dbReference type="OrthoDB" id="1705869at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666272" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="126"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="25014" checksum="BDB5E6072D71FC56" modified="2014-01-22" version="1">MPSPVIFGLSVTALLALFGVGAAVLPESLTGPGTPAELPEAAVAALAPGAGGGGHPGRPGAARPSSTPVPSATPSSAAPAPSPKKTASKKPATRKPTPKKTPTRKPTTSTPKVGLGLAAQESEVIRLTNVERDKAGCGSVSLNTKLRTAMRLHVQELGTHGDLYISHVSDDGRDFATRAKEQGYNDPGGENVARGQQNAADVMNSWMNSEGHRANILNCSFEAIGVGAVKGVDGTIVWGQIFGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCT4</accession>
    <name>U5WCT4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562150.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37805"/>
    <dbReference type="EnsemblBacteria" id="AGZ45815">
        <property type="protein sequence ID" value="AGZ45815"/>
        <property type="gene ID" value="AFR_37805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7645"/>
    <dbReference type="eggNOG" id="ENOG5033E2A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_789017_0_0_11"/>
    <dbReference type="OrthoDB" id="2084525at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="346" mass="36355" checksum="DCB55F71A7512B83" modified="2014-01-22" version="1">MTAPLELRYRRLLAVYPADHRRIYAEEMVGVLMEGAEPGQQRPALAEAANLVWSGLIARLGRGAQGLRSALWRDAAAVAALAGVVMLLFVPARRLYFGLQNFVNFGDPMRAYGFDGGLLIDVAARSAIWLAVLVAVLLAAKRMSVALGVAGLLVEVAALVVRAFVVEFHAIRMSWVLALAGATLTLLVLARSGRPVTGMLGRRGRAMLAAGVATAGVTASALPGDRSPVQILGVIEVTDALYLVAGGLLLAGVHRVPGRVRRRALVLIAPLLAVPWAQHLLEDAISMSTARYVSTGMIVADVVIMTVVPAAVLAAALGVLFLREQVVVSVTRSRPDAETGAPASSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEI7</accession>
    <name>U5WEI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flagellar GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">flhF</name>
        <name evidence="2" type="ORF">AFR_40535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40535"/>
    <dbReference type="EnsemblBacteria" id="AGZ46361">
        <property type="protein sequence ID" value="AGZ46361"/>
        <property type="gene ID" value="AFR_40535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8204"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_519388_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="527" mass="53762" checksum="F2E1B6DB8D58AABB" modified="2014-01-22" version="1">MLLEGPAIEPLLAQVRDEYGSSVRIISADKVRSGGFGGFFAKQHYELSVEVPDPEERNEPQRRQPAAEEPPQTLEQLLARAESRDRIAPDDNPAGGDRRTGRDAGMGDADAAVGRPSLGGGGAGPNLGGGAGRALADPGAAFAELMAGLDKADTNAWARPAPRPHADAEPERPENPSVRPFRPAQATGSPVNGGSHSGPSVRPLPPVPSLAELMGGLGVSDGPAFPTNAAATRAAQNPAPPRTPAAAYGLAPTSPAPAGNGLAETLAGLGLGAPQAQTPLHATTTTAQPPATLRPMHADPVQQNLMMVGMPAEMAAQIVGGDTYAGVLSVLASRPAAPGVPDAPGEILVLCGDLDHALPIGKKLCEQSGIDQAHLLLGGPTAAGTGMHSSRVLGDPTAAATKAERLQNADHAWIVVVDAKVGGTDPIWVSDMCDAIGATAVWAVVDATRKTADTARHLRTLGDIEALVVHSVDITADPATVLGLDLPIVSLDGKPATPHAWAAMLCARIAADVVPMAALPRRPARGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEP0</accession>
    <name>U5WEP0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CarD family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562842.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41280"/>
    <dbReference type="EnsemblBacteria" id="AGZ46510">
        <property type="protein sequence ID" value="AGZ46510"/>
        <property type="gene ID" value="AFR_41280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8354"/>
    <dbReference type="eggNOG" id="COG1329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048259_1_2_11"/>
    <dbReference type="OrthoDB" id="1942592at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042215">
        <property type="entry name" value="CarD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CarD_TRCF" evidence="1">
        <location>
            <begin position="2"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17853" checksum="0DCE30252B73427B" modified="2014-01-22" version="1">MVFSVGETVVYPHHGAALIEAIETRTIKGVEKKYLVLRVAQGDLTVRVPAENAEIVGVREVVGEEGLGKVFDVLRAPHTEEPTNWSRRYKANLEKLASGNPLKVAEVVRDLWRRERERGLSAGEKRMLAKARDILVGEVALAEKSTKDEAEVLLDKVLTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNU8</accession>
    <name>U5VNU8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Deoxyribodipyrimidine photo-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_00900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DNA photolyase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357412.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00900"/>
    <dbReference type="EnsemblBacteria" id="AGZ38469">
        <property type="protein sequence ID" value="AGZ38469"/>
        <property type="gene ID" value="AFR_00900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.179"/>
    <dbReference type="eggNOG" id="COG0415">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010348_2_2_11"/>
    <dbReference type="OrthoDB" id="184000at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901363">
        <property type="term" value="F:heterocyclic compound binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097159">
        <property type="term" value="F:organic cyclic compound binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051716">
        <property type="term" value="P:cellular response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018394">
        <property type="entry name" value="DNA_photolyase_1_CS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="PTHR11455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="SSF48173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="SSF52425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00394">
        <property type="entry name" value="DNA_PHOTOLYASES_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00691">
        <property type="entry name" value="DNA_PHOTOLYASES_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 5" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="7">
        <location>
            <begin position="2"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="444" mass="49465" checksum="0D54183BFABDD22B" modified="2014-01-22" version="1">MRTAVVLFTRDLRVHDNPALASACANAEQVAPLFVLDPRLQTLSPNRARFLHQSLADLRETLRAKGGDLIVRTGDPVVEAIKLAREVGAEGIALAADVSAYARRREQRLEQECEQHRISLRLFPGTTVLPPGGVQPGGGGGHYKVFSPYYRAWESAKWRDEVATPRTITLPPDVTVGDLPEAPAGDSPAAALGGETEGRRRLETWLQHVGPYDDLHNDMPADGTSRLSPYLRFGCVSPLTVANLARAVDGPGPAAFVRQLGWRDFYYQVTASFPAISTQAYRKSGDGEWRNDDDALKHWQEGTTGVPVVDAGMRQLRAEGWMHNRARLITASFLTKHLGLDWREGVQWFFRFLLDGDVPNNSGNWQWVAGTGNDTKPYRRFNPIRQAERFDPDGVYVRRYVPELKAVEGKAVHQPWRLPEAVRSGLGYPGPLESHRDEAVWLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP69</accession>
    <name>U5VP69_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transmembrane anti-sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357706.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02370"/>
    <dbReference type="EnsemblBacteria" id="AGZ38763">
        <property type="protein sequence ID" value="AGZ38763"/>
        <property type="gene ID" value="AFR_02370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.479"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056526_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041916">
        <property type="entry name" value="Anti_sigma_zinc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027383">
        <property type="entry name" value="Znf_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13490">
        <property type="entry name" value="zf-HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="zf-HC2" evidence="2">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="24041" checksum="B6C1C0C10A51560B" modified="2014-01-22" version="1">MNTNHVDLAGYLTKSLDAEQIRQVEEHLAGCAECRAEVESLQEWTMALEAVPEAMLLDGPPEGGDLLLQRTLRQIRDESSGRRHRRNALVGVAAAVVVAAAIASGAAVGRLSVDPAAPVALPTPVASEPTTIPGTKTATAVDAGTGARITVAVAPAPGWVRIKAAVVGIPAGERCRLEVIGKDGTTVLAGSWVVSDKGAAEGTTLDGSALVPPDQVSSVRVVTEAGKQYTSVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPZ8</accession>
    <name>U5VPZ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357973.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39028">
        <property type="protein sequence ID" value="AGZ39028"/>
        <property type="gene ID" value="AFR_03695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.744"/>
    <dbReference type="eggNOG" id="ENOG50313PX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811646_0_0_11"/>
    <dbReference type="OrthoDB" id="1968009at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="15744" checksum="A626ABC3260FE66D" modified="2014-01-22" version="1">MTFRYLAAPGPLELTFGVRLWAFEPGHSKLLLRGFSDPESEDGQTIVDVVFSNVTRMRLDAGYPGLMVRVAEESDAIPQAEIGRTFTDDKVYAVNPGPRYGFVVAERVDWATLDIQGHEPSPLLAEDTARLTDVAVGDIYWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQI3</accession>
    <name>U5VQI3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358014.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03900"/>
    <dbReference type="EnsemblBacteria" id="AGZ39069">
        <property type="protein sequence ID" value="AGZ39069"/>
        <property type="gene ID" value="AFR_03900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.788"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060397_1_1_11"/>
    <dbReference type="OrthoDB" id="1865374at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="65"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="24117" checksum="73E24ABD468F5B43" modified="2014-01-22" version="1">MLEGGNTSGPGESTDGWLVDTRTSYDTVAGSYADFVRDALAREPYLRAALALFADLVHAAGGGPVADLGCGPGHVTAHLHNLGVEAFGVDLSPTMVDIARQDHPGLRFEVGSMTDLHLADGSVAALLAFWSLIHIPDDAIPTALNHFRRVLRPGGPVLIGFHVGNESRLKTEGYGGHPMKVYVHRRQPEQMSAWLRNAGFTLNAQMVLDPDGSSPGAILIAHLTPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR21</accession>
    <name>U5VR21_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05670"/>
    <dbReference type="EnsemblBacteria" id="AGZ39423">
        <property type="protein sequence ID" value="AGZ39423"/>
        <property type="gene ID" value="AFR_05670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1150"/>
    <dbReference type="eggNOG" id="COG3824">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118049_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010428">
        <property type="entry name" value="Zincin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038555">
        <property type="entry name" value="Zincin_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06262">
        <property type="entry name" value="Zincin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="13617" checksum="CB353EF131926199" modified="2014-01-22" version="1">MPATVPLARTKAEIFDDLVLDTVESLERRYAKELAGVEFAVEDVPPELNVYDSDVLEDGEVPLARLLPGRPGRQELPPRIVLYRRPLEFRAMDREDLADLVHDVIIEQVANLLGVDPDELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR33</accession>
    <name>U5VR33_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358821.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05100"/>
    <dbReference type="EnsemblBacteria" id="AGZ39309">
        <property type="protein sequence ID" value="AGZ39309"/>
        <property type="gene ID" value="AFR_05100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1031"/>
    <dbReference type="eggNOG" id="COG2311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033238_0_0_11"/>
    <dbReference type="OrthoDB" id="1854931at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045931">
        <property type="entry name" value="DUF6350"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19877">
        <property type="entry name" value="DUF6350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="483" mass="48573" checksum="731B3C680F27FCD6" modified="2014-01-22" version="1">MPTTPDRPDGRPAGSEDQFEAAEAVHLAARETVPVTVRRSTPGDHSTVKINPADRSTVRIDPRNHPTVKLPGQRRPVDSRGRAPLPVAAAFATFWAAFVAYVPVAAVVGLARTLEGQGGLGGAAHAGLAGWLLGHGVPIGTSIGPVGVAPLLLTLLAGWRLNRAGLHVTRAIGARRSGSPRVALLVAGTIGVAYAILGLLAALVVDGRGTDVSPTRAALNFFLAGLAFSLIGSLRGTEALVVVARRISPTLRHGLRAGVMSALLILAAGAAIAGLSVAVGGGQAADMISAYRTGVAGQAGITLVSLAYGANGVIWAAAYLLGPGFLLGTDSVVRLTEVTVGPLLPTLPLLAGLPNGPIGASGAALLAVPVLAAMASGWLLTRRLVSIHHVVDGHLVHGRNPGDPAPAEPSWSLVLGAAVLSGPVAGLLLGLLSWMSGGSMGNGRLAEIGPVPWQVTLVATIVVAVSASIGAAAGRAFRAPARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTB3</accession>
    <name>U5VTB3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_03060</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03060"/>
    <dbReference type="EnsemblBacteria" id="AGZ38901">
        <property type="protein sequence ID" value="AGZ38901"/>
        <property type="gene ID" value="AFR_03060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.616"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032067_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="463" mass="51515" checksum="20E2CEECA51358BB" modified="2014-01-22" version="1">MSGIGAAYRLQTESPGRSYAILEARETSGGTWDLFRYPGVRSDSDMFTLGYPFRPWREPKSIAAGRTILDYLRDTAAENGIDKHIRYGRRVVSAAWSTPEARWTVRTATGEEWTCSFLYVCTGYYRYDSGYEADFPGRESFTGPIVHPQHWPEDLDWTGKRVVIIGSGATAVTLVPAMADRAAHVTMLQRSPSYLLALPTREGAARRVGPHVVRARNVLLTWALYQAAQRWPDRVARALKKGVAEQLPPGVDVDPHFSPRYAPWDQRLCVVPDGDLFAAMRAGQASVSTGTIESFTPDGIRLTGGEHLDADVIVTATGLTMVAFGEIALTVDGREINSADLHVYKGMMFDGVPNFAWCVGYTNASWTLRADLTSRYVCRLLNYLARHRIDIATPTLPADAHDTGEPLMALSSGYVQRAASVLPRQSDRRTWRMINNYLLDLPRMRLSRIDDGDMMFARIKTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTI6</accession>
    <name>U5VTI6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359773.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09375"/>
    <dbReference type="EnsemblBacteria" id="AGZ40164">
        <property type="protein sequence ID" value="AGZ40164"/>
        <property type="gene ID" value="AFR_09375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1911"/>
    <dbReference type="eggNOG" id="COG0645">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116774_0_0_11"/>
    <dbReference type="OrthoDB" id="1574819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="18458" checksum="89CE068D554D3240" modified="2014-01-22" version="1">MVLAVFAGLPGVGKSTLAQQVGAVLPAAVLAVDTVDFTMQSYDVSEPRPGYAAYGVVAALAETQLRMGHHVIIDAVNPVKAARQLWTELAENLGVPLRVVEVVCGDDAEHRRRVETRFAACAHDGIPDWVRVLERQAEWEPYLGPRLVVDTFLAEEPVGPVVDYLSLRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTQ9</accession>
    <name>U5VTQ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zeta-phytoene desaturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_09825</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 3">Carotenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the carotenoid/retinoid oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09825"/>
    <dbReference type="EnsemblBacteria" id="AGZ40254">
        <property type="protein sequence ID" value="AGZ40254"/>
        <property type="gene ID" value="AFR_09825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2003"/>
    <dbReference type="eggNOG" id="COG1233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019722_2_1_11"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014105">
        <property type="entry name" value="Carotenoid/retinoid_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02734">
        <property type="entry name" value="crtI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="476" mass="49830" checksum="B06B82A1F47D27CC" modified="2014-01-22" version="1">MVIGAGVGGLAAAVRLAEAGHDVVVHERAATVGGKLGRYERDGFRFDTGPSLLTLPQVFADLGLGLDPVALDPVVRHVFPDGSVLDSSSDPAVFRERIENAFGAAAGADWARFWARAERIWAASWRSVLQQEVTPATLAALSWRVGDLAAIAPWRSLRGLGRHYLRDPRLRMLLDRYATYTGADPRRAPAALAAIPYAELAFGGWYLPGGLGVLASALLDRCAALGVRITTSSPVAGITTTAGRAAGVRLDSGEVVAADVVVSNVDAVTTYRELLPSPGRLAGLADRSLAGFVILLGVRGSTPELAHHTVFFPGNYNAEFDAVFGSPGTGVRARPASDPTIFVTRAEDPAVHPPGTEAWFILVNAARHGTDFGAVDWRRPGLAQAYADHILKRLAARGVDVRDRVLFAETRTPADLAESTAAPGGTIYGTAGGLLRPGNRGPVDGLFLVGGSTHPGGGLPMVTLSAKIVADEIGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTS7</accession>
    <name>U5VTS7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09925"/>
    <dbReference type="EnsemblBacteria" id="AGZ40274">
        <property type="protein sequence ID" value="AGZ40274"/>
        <property type="gene ID" value="AFR_09925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2024"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026152_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="190"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="422" mass="44939" checksum="F4FD9CA10E6FA5F9" modified="2014-01-22" version="1">MTTRGRSFLAAAAAAGISAIVLGEKDLLRVAILLAALPLLAAAYVGRSRYKLACTRSLEPGRAPVGSSARVILRLQNMSRLPTGTLLLEDRLPYALGSRPRVVLERLGAHQASSVAYTVRADVRGRYPVGPLVIRLTDPFGLCELTRSFPSVDRLTVIPQVVPLPAVRLAGEYAGTGDSRARSVAVHGEDDAATREYRRGDDLRRVHWRSTARTGELMVRREEQPWESRATVVLDTRLDAHRGEGPTASFEWAVSAAASIAVHLRQSGYKLRLVTGTGIDVDATEIGGEGAILDTLADVKLSAAGDVSVLVEQVRRRSDGGLVIGLFGTLSVAEAEVLTGLRGNGATCIGFAIDSSTWVNMLPPDRTEADRLHGSTALALVRSGWRSVPVAHGDSLAALWPAAGRGSQGFAYRAAMAETVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU10</accession>
    <name>U5VU10_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40359">
        <property type="protein sequence ID" value="AGZ40359"/>
        <property type="gene ID" value="AFR_10350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2108"/>
    <dbReference type="eggNOG" id="ENOG502ZD0Q">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2091553_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045428">
        <property type="entry name" value="EACC1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19953">
        <property type="entry name" value="EACC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="116" mass="12899" checksum="E015948FA1BA4930" modified="2014-01-22" version="1">MVTLQVEEQSHQDLSDWLRKEPLLSSARFGTTGAPPRPGEMGWPADALQIVANADLLTAVATTLGVWLGTRVRRTKIKITHKGRTIEVESGKNAEAITREILDRLDLLDELDEDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUA4</accession>
    <name>U5VUA4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360351.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10850"/>
    <dbReference type="EnsemblBacteria" id="AGZ40459">
        <property type="protein sequence ID" value="AGZ40459"/>
        <property type="gene ID" value="AFR_10850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10850"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_11"/>
    <dbReference type="OrthoDB" id="1209997at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="25196" checksum="10E0821030EFFCFA" modified="2014-01-22" version="1">MDLGLADRVYVLTGASKGLGFATAQSLVADGAKVVISSRSQDSVDAAVARLGAGAAGVAADLADPGTPGLLVSTARERFGRLDGALVSVGGPGSGTAAQMTDDQWRQAFETVFLGTVRAMRTFAAELGEGGAIAVVLSTSTRTPIPGLGLSNGLRPGLAGVAKDMADEFGPRGIRVVSLLPGRVLTDRNRDLFAATGDSEAAATSAAASVPLGRLGEPEEFGRVAAFVLSPAASYLTGLTLPVDGGALRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUA5</accession>
    <name>U5VUA5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rpmJ</name>
        <name evidence="6" type="ORF">AFR_04635</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358216.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04635"/>
    <dbReference type="EnsemblBacteria" id="AGZ39216">
        <property type="protein sequence ID" value="AGZ39216"/>
        <property type="gene ID" value="AFR_04635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.936"/>
    <dbReference type="eggNOG" id="COG0257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135723_6_2_11"/>
    <dbReference type="OrthoDB" id="2066169at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_L36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_L36_sp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42888">
        <property type="entry name" value="PTHR42888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="SSF57840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00828">
        <property type="entry name" value="RIBOSOMAL_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="37" mass="4429" checksum="C935649F436F6B6F" modified="2014-01-22" version="1">MKVKPSVKRICNKCRVIRRHGRVMVICDDPRHKQRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUN2</accession>
    <name>U5VUN2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358887.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05335"/>
    <dbReference type="EnsemblBacteria" id="AGZ39356">
        <property type="protein sequence ID" value="AGZ39356"/>
        <property type="gene ID" value="AFR_05335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1080"/>
    <dbReference type="eggNOG" id="COG1507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097805_1_0_11"/>
    <dbReference type="OrthoDB" id="1922611at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007511">
        <property type="entry name" value="DUF501"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37163">
        <property type="entry name" value="PTHR37163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04417">
        <property type="entry name" value="DUF501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="18188" checksum="579A8B6013C75092" modified="2014-01-22" version="1">MSTPSESDLEIVAQQLGRRPRGTRAVAHRCPCGNPDVVETSPRLDDGTPFPTMFYLTCPRATAACSRLESAGVMRDMQDRLSTDPVLAKQYELAHEDYLRRREAVEHVEEISKVSAGGMPDRVKCLHVHLGHALAAGPGINPFGDEVRAAVEPWWTGGPCVRPEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUR6</accession>
    <name>U5VUR6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme F420-dependent NADP oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40733">
        <property type="protein sequence ID" value="AGZ40733"/>
        <property type="gene ID" value="AFR_12235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2487"/>
    <dbReference type="eggNOG" id="COG2085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124419_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="14096" checksum="8B6E873898318C0F" modified="2014-01-22" version="1">MEAALSSVVGIEGKTVLDATNLYGGTKPPAGFASNAEYVRSVTGGPTAKTFNANFASLFDRVPDARVWPSNLWSGDDQAKDVVERLSLDAGYEAIRLGQLTAASIQEGLAGVLFGIAQSGLGEFVYRMAPPEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUS6</accession>
    <name>U5VUS6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-polyprenyl-6-methoxyphenol hydroxylase-like oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358937.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05585"/>
    <dbReference type="EnsemblBacteria" id="AGZ39406">
        <property type="protein sequence ID" value="AGZ39406"/>
        <property type="gene ID" value="AFR_05585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1133"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_1_0_11"/>
    <dbReference type="OrthoDB" id="504558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="5"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="388" mass="42248" checksum="54F5FB4E5BCF06B7" modified="2014-01-22" version="1">MPGRTVLISGASVAGPALAYWLEAAGWQATIVERAAELRSEGQNIDIRGAAREVVRRMGLEDTIRAAGTGEEGTRIVDAGGRTRASFPATRSDTAGATAELEILRGELSRIVVAQTSAEYLFGTTITAVHEDEDGVAVSFTDGPDRRFDVVVLAEGMRSRTRTQVFGTEARIRHLGMYTSYFTIPKKPGDPDWWQWYNAVGGRNLGLRPDNLGTMRATLSFLSPPRGYEDLGQDEQKQLLHRVFRDAGWEAPRMLAAMDDAELYFENLGQVFAPTWHRGRIALLGDAAYCASPLSGMGTSLALVGAYVLGGELSRATSHREAFANYERLMRPYVDKAQKLIPGGPRLANPKSRLGIKVLQTTMGVAARIGSLSLLTPPADKFELPTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV09</accession>
    <name>U5VV09_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Extracellular small neutral protease</fullName>
            <ecNumber evidence="3">3.4.24.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Snapalysin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_06010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.</text>
            <dbReference type="EC" id="3.4.24.77"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M7 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.77" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359022.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06010"/>
    <dbReference type="EnsemblBacteria" id="AGZ39491">
        <property type="protein sequence ID" value="AGZ39491"/>
        <property type="gene ID" value="AFR_06010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1221"/>
    <dbReference type="eggNOG" id="COG5640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124996_0_0_11"/>
    <dbReference type="OrthoDB" id="1649119at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000013">
        <property type="entry name" value="Peptidase_M7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02031">
        <property type="entry name" value="Peptidase_M7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00787">
        <property type="entry name" value="NEUTRALPTASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665245" description="Extracellular small neutral protease" evidence="6">
        <location>
            <begin position="30"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="176" mass="18950" checksum="FB61254C948A05BB" modified="2014-01-22" version="1">MLRQRLSAALLAIAMLVGLQVATTGPAAAAPRILYYDASQAQEFKAVVAQGAQIWNSRVANVQLQPVPAGRTPNIRVYADNGWPRTYSTSLGNGRWYMGREAVNDGYYQPRIAAHEFGHILGLPDRRTGLCSDLMSGSSAPVSCTNPNPNSTEASTVNNLFRSAAAVPAREFTWVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVA3</accession>
    <name>U5VVA3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12590"/>
    <dbReference type="EnsemblBacteria" id="AGZ40804">
        <property type="protein sequence ID" value="AGZ40804"/>
        <property type="gene ID" value="AFR_12590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2557"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_570656_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="478" mass="51037" checksum="8A775657FEE8EF59" modified="2014-01-22" version="1">MSMKDKPLWSRRGVLGMGVGVLGAAGLAACGDTSESPSTALPEVPKELVDAAAAVKGTSMGVLSQKLYSAAANEALDNSIKKFADSTGTKIGNNLIQADAGDVVAKIDAEVKGGVARDLAFMTDSRFIAQFHALGDLEDVTDVVEALTAKYGEPCAEAKNFCVFDGKWFAIPYHFIGIGSLLRKDWMQEKGITAKDVYTWEELRDLCLSISDPAKRRFGWGMTVNRSGDGNGMIEALINSYGGAITTNDGRKVTFDSPETVQAVTFLGDIYTNPKFKPMLPPGVASWTDTSNNENWLAGILGYTRNSFSIYADSKTKKNPVYANTHVFSDCIGPATDKSLLLGQSQGFVVFKGAKNPTLAKLLAQYLITAPALVGVSKEAPGLVMPAWEKVWDADPYFASGDPAFPLLRNMSQQPLPLSTKNGLAFPQKPSAGQQAVVAAYVLTDMMQQVVQGTAPAQAVTAAHAKMVQIFAQQGLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVS1</accession>
    <name>U5VVS1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359351.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07655"/>
    <dbReference type="EnsemblBacteria" id="AGZ39820">
        <property type="protein sequence ID" value="AGZ39820"/>
        <property type="gene ID" value="AFR_07655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1561"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2115765_0_0_11"/>
    <dbReference type="OrthoDB" id="1864829at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="16"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="114" mass="12144" checksum="8F2789F01BF01A54" modified="2014-01-22" version="1">MTDQWVTFAKIGTADLVRLAGEIDLANAPEIGREIVRHTGRAGAVVIDLTAVSFLDSAGVRLLDALVGDLDDQGTPIRLVVGETGPARMTLQLCAFRDDLLATDLDRAAEDIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW42</accession>
    <name>U5VW42_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_14605</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361262.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14605"/>
    <dbReference type="EnsemblBacteria" id="AGZ41203">
        <property type="protein sequence ID" value="AGZ41203"/>
        <property type="gene ID" value="AFR_14605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2966"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_0_11"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="95"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="335" mass="36620" checksum="3788EF2F39D6A355" modified="2014-01-22" version="1">MVRVILRRLVQLVVTLIALMTLVFFWLRSLPGGPVDALLGERATPQTRELLTRALGYDQPIWVQYGRFLKNVVTGNFGNSIRTGEPVMDVISRSFPATIELAVAAIIIAIGLGIPLGYVAARYRGRLLDNATIIVTLIGISIPIFFLGYLLKDWLTQDIHFFPPSGRITTGLGNTDVTGFFVLDGLLTREFDASADALWHLVLPAFTLATIPLAIIIRITRASVLDVLDEDYIRTADAKGLRNATIRKRHVLRNALLPVVTTIGLQTGALLAGAVLTEKVYNWGGLGTLITDSISGGRDYPVLQALILLAAVVFIVVNLLVDLSYAIIDPRVRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW82</accession>
    <name>U5VW82_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyl-accepting chemotaxis protein mcpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08160"/>
    <dbReference type="EnsemblBacteria" id="AGZ39921">
        <property type="protein sequence ID" value="AGZ39921"/>
        <property type="gene ID" value="AFR_08160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1665"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="214"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="278"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="529" mass="54313" checksum="39A54D1D76213424" modified="2014-01-22" version="1">MESQQHVRSRLTIVARLVLMAAIGVASVALVGAASSWASENQSDATDRIAAISDGMSSQWNADMMHDGLRADVMSALFATTAAQREALAVSEVTEHATTLVTKYDAAATAAPTALGDDYTRVRPAVVAYGKTAEGLVALAATDHAAAEAELPNFLKAFGTLEEELGAIDDAMLTAVQDAGREGTETGRTSGWLILVAGLAGALLTAAAALFTVRAVRRPLRQMVAALRAAAGRDLTVEVEVVRPDELGEMAVALNEALSAIRTTVAATAASVGTLTAASSDLRDLAGRLDSSAEQTSAQARSADTAAQQVSHSVTDMMTATEELSASIREISQQTTTAATTTAAAGENAAATTALVATLSEASREIGTIVQLITSIAEQTNLLALNATIEAARAGDAGKGFAVVASEVKDLAQETAQATSDITGRIAAIQDMTSRTATAIDAIAEVITRIDDGQRTIAAAVEEQSTTTELMARNVGDMSIAATEISGTVTHITTSTGATADGANTTRRSAERVSTAAGEIQVLIGQFTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW89</accession>
    <name>U5VW89_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein icfG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361168.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14135"/>
    <dbReference type="EnsemblBacteria" id="AGZ41109">
        <property type="protein sequence ID" value="AGZ41109"/>
        <property type="gene ID" value="AFR_14135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2868"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043255_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="172"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="42825" checksum="BCE3364A715422DA" modified="2014-01-22" version="1">MGTLDAVRQLIRQGYRARPEDLADMAMQAAPLLGVTAMVIYVVDHQQRQLLPLLGDQSPPCEPTAVDGTLAGRAYSSLTPVAASPDDGRQVVWVPLLDGSERMGVLQVIADRPLTEEAVADCATIAALVGEIVVTCDLYSDTIERRRRREPMQLAAEMLRAQLPPLTFATSRLVVAGILEPCYDVGGDAFDYAVNGDTAHLALFDAVGHGSAGGMRAVVLASIAIAAYRNARRTGMDLASTYHHLDNAVRDHDRTGMITAVLAELDQNNGVLHVISAGHPGGIVLRNGRLVTVLPSPTALPVTLGDRRPPVIIQEALEPGDHVLLYTDGITEARDKDGTPFGLDRLVEFTQQALADQLTAAETARRLVHAILGHQDDCLQDDATVLLLHWLGPVAAPPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWM5</accession>
    <name>U5VWM5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative LuxR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361251.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14550"/>
    <dbReference type="EnsemblBacteria" id="AGZ41192">
        <property type="protein sequence ID" value="AGZ41192"/>
        <property type="gene ID" value="AFR_14550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2955"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_4_1_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="818"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="884" mass="94021" checksum="01650D776B238C94" modified="2014-01-22" version="1">MTAIIGRDAQIGTLTALLDDAVGGQGRALVVRGEAGIGKSALLQLAAGLAGGRGIRVLSASGVQAEVHIPFAGLSQLVRPLPLDLESPFRTAVELLERLSSDAEPVLLAVEDAHWLDRASWDVLTFLARRIESDRIAVIMTARDGVDVDQRLAGAGLPELRLEPLSAADAGALLDRTAPGLAAALRTRVLDEAAGNPLGVVELGAVAARSGGAALLPSWLPLSTRVERTFAGLVAELPEPTRTLLLVAALDDGDDLDEVLAAAGRSVGRVILADDVQPAVVTRLVTVDDLYRIRFRHPLLRSALRQSAGAAQRRRVHDALAAVVADPDRQLWHRAAAAAGPDQALADELAALAVRVRDQQAVAAALAAMERAAQLSEDPEARGRRLMLAAYLAHDQGDAEGVRRLSTEIDDRQLRPGDRARLAWLREVYLASGWTGGRQLRGYADLIDTMRRDGDAGAAVDSLASVALRIFYSAIDDETRDRFLAVADAIETPARDPRIIAALATIAPVDRGAACLGPMAALLTRLDLTTAERHDLAVGSSAMGAYEISTAFARSAVPDLRAQGRIGSLAMLLVNLSFVTAGTGDTRAAVQAGEEGVSLAQETGQPSWALTGRLCVGLADALRGNGDAALVAADLGESVLLPAKMHPMLCLVQQIRGVQALAAGRPEDAYRQLHRMFDPGDITYHPYVRFGMVGHLAEAAVLSGAQDELRGVVAELEPVAERSLAPSLIAGLCYARAVLADDADGYRAALATDLSAWPLEQARLQLAHGTWLRRQRQAAESRPVLRAAAASFDALGVTPWADRARAELRATGETRRKPLDAIDELTPQERHIARLAAEGLSNREIAERLFLSPRTVSTHLYRIYPKVGVKSRGELAAFLTERPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWN6</accession>
    <name>U5VWN6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359572.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08760"/>
    <dbReference type="EnsemblBacteria" id="AGZ40041">
        <property type="protein sequence ID" value="AGZ40041"/>
        <property type="gene ID" value="AFR_08760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1782"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_12_11"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033462">
        <property type="entry name" value="Cache_3-Cache_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17201">
        <property type="entry name" value="Cache_3-Cache_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665424" evidence="4">
        <location>
            <begin position="37"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="388"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="445"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="703" mass="70979" checksum="2CCCEC820C64FE1C" modified="2014-01-22" version="1">MGFQISHKLLALGLGGVVSTAAVLVGVGAWQSDAFADRTNAQVSRLNAEDLSQTTANVTRLVSSVGDEVQSGVDGDMRAAGTLLGQRGGMRLDGDTATWTATNQVSQAKRSVTLPRATVDGTWLGQNTDTKARTPFVDDVKSLLGGAVTVFQRMNDAGDLLRVATTVQAKSGNRAIGTYIPAVGADGTPNAVAAAIKAGKPYRGVAQVVDTWYITAYDPIKDAGGRVIGALFVGVPQADALKNLTEAITATSVRENGWVTVYSTAKADAGRVISSSLDGAGGLDATDADGTKYVEQIVAQAPKLTGGTTWAATYRLPGSGGAPAGPTTTTVAFYAPYSWAIAIGGYDADAAGAVEAVHDGRRSMLTAFLIAALLLAVAGGLIAAWQARRISGRLGGLTRALTRLAGRDLTVSVPPSGSDEIGRAGAALNTAVAELRGVIVEVSGASEGVSRSAGEVAATGGELASSAQDASGQAQAAGLAAEGITHVVQTVAAGAEEMGASIGEISSNAQDAAQAGRDGVGLTETATGVISELRVSTTQIADVVRLIAGIAEQTNLLALNATIEAARAGDLGKGFAVVAGEVKELAQETARATGDVTARVAAIEGDTARAVEAINAITATIGQVNDYQTAIAAAVEEQAATTAEMARNISEVATGSQDISIGITAVSGAVDSTRSAADVSHRAADELNATAHRLTELVGRFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX12</accession>
    <name>U5VX12_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ubiquinone/menaquinone biosynthesis methyltransferase ubiE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15635"/>
    <dbReference type="EnsemblBacteria" id="AGZ41409">
        <property type="protein sequence ID" value="AGZ41409"/>
        <property type="gene ID" value="AFR_15635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3175"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1137396_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0830">Ubiquinone</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="22616" checksum="8F71317168AF1862" modified="2014-01-22" version="1">MIDEDTLLGSAVVANNAMNRERQLTGANSYAKELGFDPVDLLGENSAWLDLCCGSGRALIQAATRVRAAALVGVDLVDAFDPVPGGAPPELICAPLLSWTPNRTFDLITCVHGLHYVGDKLAALGRAASWLSPGGRLVADLDLSAIDTGEPTTRRLASRLRAAGFTYDARRRRISRTGPAVLNLPYVYLGADDRAGPNYTGQPAVRSHYRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX81</accession>
    <name>U5VX81_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydroxyacetone kinase subunit DhaL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16380"/>
    <dbReference type="EnsemblBacteria" id="AGZ41558">
        <property type="protein sequence ID" value="AGZ41558"/>
        <property type="gene ID" value="AFR_16380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3322"/>
    <dbReference type="eggNOG" id="COG1461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066424_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012737">
        <property type="entry name" value="DhaK_L_YcgS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="SSF101473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02365">
        <property type="entry name" value="dha_L_ycgS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaL" evidence="1">
        <location>
            <begin position="19"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="22433" checksum="8EA4151C7ABD1CE1" modified="2014-01-22" version="1">MGRTGADAGAAVVTAVENAALTAWIREFARLIAEAKERLTELDSAIGDADHGSNLDRGLTAVVAMLDAEAPDTPGQLFKRTGMTLVSTVGGASGPLYGTAFLRMAAAAGAGSSLDPPEFAQVLRAALEGVVARGKAVAGDKTMYDALAPAVDALDAALAAGKSLGDALAAAVKAAEEGTAATIPMVARKGRASYLGERSAGHQDPGATSMTMLFTAAATTLSGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXR5</accession>
    <name>U5VXR5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360356.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10860"/>
    <dbReference type="EnsemblBacteria" id="AGZ40461">
        <property type="protein sequence ID" value="AGZ40461"/>
        <property type="gene ID" value="AFR_10860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2212"/>
    <dbReference type="eggNOG" id="COG2329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125060_0_0_11"/>
    <dbReference type="OrthoDB" id="1907687at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="13029" checksum="87BAACE659AFDCB6" modified="2014-01-22" version="1">MTTFDTTVQRYLDVWNTTDPAERLSRITEVFTPDVRYVDPLAAVEGHEALNQLIGAAQGQFPGMVFTPGGPSDAHHDQARFTWQLGPAGGEALVIGFDVAELGSDGRIRTVLGFLDRVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXT5</accession>
    <name>U5VXT5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase GCN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361647.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16450"/>
    <dbReference type="EnsemblBacteria" id="AGZ41572">
        <property type="protein sequence ID" value="AGZ41572"/>
        <property type="gene ID" value="AFR_16450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3336"/>
    <dbReference type="eggNOG" id="COG3153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081840_3_2_11"/>
    <dbReference type="OrthoDB" id="1687413at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="190" mass="20294" checksum="967E13C4C1DDC64E" modified="2014-01-22" version="1">MEIREVLPAEEGAAEAVVAAAFGEAPDGRVVQMMRALRAGGAARASLVAVAEGEPAGHVGLSRAWVDARRELVEVVVLSPLSVRPDLQGRGVGTALVAAALEASEDRGTPAVFLEGSPEYYGRRGFRRASALGFDRPSTRIPDPGFQVALLSSYQPWMVGRLIYPEAFWTTDTVGLRDPELERIEALIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYB4</accession>
    <name>U5VYB4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Group 1 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360665.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11710"/>
    <dbReference type="EnsemblBacteria" id="AGZ40631">
        <property type="protein sequence ID" value="AGZ40631"/>
        <property type="gene ID" value="AFR_11710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2385"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_11_2_11"/>
    <dbReference type="OrthoDB" id="1591999at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="3">
        <location>
            <begin position="18"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="420" mass="46584" checksum="83E90E205890CD31" modified="2014-01-22" version="1">MRIVYIHQYYCNPQMTGGIRSYEQARRLVARGHEVHVITTDITPGKRSLGWRRTDDDGVNVHWFSVPYSNNMSFTRRLRAFGEFMALATARAAKLKADLVFATSTPLTVAVPGVIAAKLRRVPFVFEVRDLWPEVPIEMGALRNPVLRRLAGALANFAYRNAEEVVALSPGMAAGVTARRPGTRTTVIPNAADLDLFAVEPAAVQRFRAENRWLGDRPLVVYTGALGAVNGVGYLVRAARRMRELDPEVQFLIVGHGKEWEDTRRLATGYGLLDRSVHMWEKVPKADLPVILGAATMSTSFVRPIRALWENSANKFFDALAASRPIAINYGGWQSDLLRETGAGLVLHPEDTDAAAAQLAGHLRDETWLQQARTAAHTLAVEQFSRDLLFDRFESVLNRAAASGRRGRRTATRAALSEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZB7</accession>
    <name>U5VZB7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YkuD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_20330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20330"/>
    <dbReference type="EnsemblBacteria" id="AGZ42338">
        <property type="protein sequence ID" value="AGZ42338"/>
        <property type="gene ID" value="AFR_20330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4122"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086383_0_0_11"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YkuD" evidence="3">
        <location>
            <begin position="126"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="26160" checksum="56A47ED62B3D1FD9" modified="2014-01-22" version="1">MVPSPPVPVLEGIAARLVARAPVPVPVAAPAPRGLPVIDYWTGPRGLPADTGQLSATGLRPASKIVVYDAPGGRPRGLVERSISGLPVVLPIVARRTGWVAVLLPTTNRRMGWVPPKGWSEEPLRDHLVVSRGDHRLTWFRHGVRKKSWTVATGAAATPTPLGRTFVMGTTGTRGAVYGGLDALVLGSIPENPEAVAEGLGAAHTGIHSWTDPGAFGRSVSNGCVRVPPKVMRLLLDEINPGTPVTVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZV4</accession>
    <name>U5VZV4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ANTAR domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_14495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361240.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14495"/>
    <dbReference type="EnsemblBacteria" id="AGZ41181">
        <property type="protein sequence ID" value="AGZ41181"/>
        <property type="gene ID" value="AFR_14495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2943"/>
    <dbReference type="eggNOG" id="COG3707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074354_0_0_11"/>
    <dbReference type="OrthoDB" id="1958442at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012074">
        <property type="entry name" value="GAF_ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036625">
        <property type="entry name" value="GAF_ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ANTAR" evidence="3">
        <location>
            <begin position="168"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="235" mass="24543" checksum="8D05AF248700ACF2" modified="2014-01-22" version="1">MTHPQAIKPTDAFAELGRIDFSETDLATALAKVADLARRTVPGADEVSITLVGPGGAHTAAFTGKRALAIDEWQYEQGNGPCLAAAAANLTVTVPDLAGDSRWPAWADHALTTGVHSSMSIGLPLRRSVSGALNVYALEPHAFDDDAVILAETFAGYAAVAMANAHLDDESAFIADHTRSAMDDSALVEQAKGIIMAEHHCTAEEASVILARLADIAGSDLHDAATALIDEAVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZZ4</accession>
    <name>U5VZZ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362831.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20935"/>
    <dbReference type="EnsemblBacteria" id="AGZ42459">
        <property type="protein sequence ID" value="AGZ42459"/>
        <property type="gene ID" value="AFR_20935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4244"/>
    <dbReference type="eggNOG" id="COG1840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_3_0_11"/>
    <dbReference type="OrthoDB" id="1567509at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="379" mass="39176" checksum="6ECCF7B70026D31F" modified="2014-01-22" version="1">MEDEVRTLARTAAALLAVTTFAGTAACSPPEKESAGASSGAASAISAADLGGLDALVTAAKAEGQLNVIALPPDWANYGEIIKAFGTKYGIKVNSAQPDASSQDEINAADQLKGQDKAPDVFDLGGAVATANVAKFAPYKPSTWADIPAELKDANGTWTNDYGGYMSIGYDAGKVPAPTSLADLLKPEYKGKVALNGDPTQAGAAFAGVVMASLGSGGTADDIAPGVEFFGKLKKAGNFLPVDPTPATVESGQTPVVIDWDYLNVAQGTKLTGKLDWKTVVPANAVVGSYYVQAISKDAPHPAAARLWQEFLYSDEGQNLWLKGGARPVRADAMEKAGTIDKTAYAALPKAEGTPVFQTEAQTAKAKEYLSANWAKAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W006</accession>
    <name>U5W006_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_15140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15140"/>
    <dbReference type="EnsemblBacteria" id="AGZ41310">
        <property type="protein sequence ID" value="AGZ41310"/>
        <property type="gene ID" value="AFR_15140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3074"/>
    <dbReference type="eggNOG" id="COG3250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009935_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Glyco_hydro_2_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="SSF49303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PA14" evidence="6">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="811"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1016" mass="109522" checksum="961902F0F8434847" modified="2014-01-22" version="1">MSAPGARDFAELGGIVLDPNIDLPGLAGTFESLTGRTEHTTARWTGKITAPATGDYTFSMIGDNGFRFLVDDQPVIDHWVPDWDVEQTSAPVHLVAGEAHTVRIEMFQDIGGANLFLRWAGAGLAKQLVPESAFTPPDGFQVFPVGLTVGADGKTLTADFDAKVDDLGQLASHLVVEVDTTPFPIKSVRLDRRDTTKVVVTLGEFVQKGQRVRVNYDGTGGLVVGGETVPQVVRSATNNSTHRLTTPFGDKVNPRHPLPEYPRPQLVRQDWLNLNGPWEFAGAEAGEQPVFGKKLGERITVPYPVESQLSGIERREDHMFYRKLVSVPKGWGGKRVKLNFGAVDYQAKVWVNGKLVTEHTGGYTAFTADITDALTRRGQQEIIVGVTDVTGPNQPKGKQSLNPGGIVYEPSSGIWQTVWLEPVATASIDQVVSTPDVATGSVVVQVKSASTSRVTVTALDKKGKKAGTVTGAANTDLRLKIANPHLWSPDDPYLYDLDVKLGSDRARSYFGLRSVAVQKVGGFPKLVLNGKPIFSLATLDQGFWPDGLYTQPSDAALKFDLVETKKLGFNAVRKHIKVESARWYRHADELGLLVWQDFVSADIDSPAGQQAWLSQGQEMMKQLHSYPSIIGWVVFNEGWGEWNREETGRIAEQLKGEDPSRILNAHSGVNCCSSKGDSGKGDVIDHHDYVNNDPAYPDATRIAMDGEHGGFTLRTPGHMWPGPPAAIYSGVADKAALTAKYVDNTERFYLEAAGAELSGSVYTQITDLETELNGLWTYDRREIKVDPAPVRAINAKVIAAGAAAGADASFPGRGDWSLDEGTGTKAADSSGSGADLTLAGNTAWAPGAKGSALKFDGDGDSADTAAPVLDTTKDYTVSAWVTLDKLPGNYASVVSQDGRATENPFYLQYGQGAFAFSTPGGNRARLAVTPDLNRWYHLTGVRDHTSGEVRLYVDGQRAAAVPAGPDVISTGPLSIGRAKYAGNRTDYWSGSIDQVHAYNRTLTDAEVAALYAAEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0L5</accession>
    <name>U5W0L5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21895</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363020.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21895"/>
    <dbReference type="EnsemblBacteria" id="AGZ42649">
        <property type="protein sequence ID" value="AGZ42649"/>
        <property type="gene ID" value="AFR_21895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4439"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="335"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="397"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="475"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="605" mass="64794" checksum="B37A5165059AAC90" modified="2014-01-22" version="1">MVRTARVLPAFLRDPGMRALAAVLVLGSLWYSFPVLGLEVLIAGFWPIQVGLDVVLALTSWRVSRDPAAPASTRRFWRSMALAAAVFTVGDVVQTVQSAADLTVGALLSGNLHLAFIVIGVSMPLWAMLTHPIRATGRERLRFWLDAATVLTAAASFLWAFLISQLMGAEPSLLVLNSLLATIMLIIFFGIAKLILGGNAPFRRVAATIALVGVVLTAIGAGLDGLGATEHPALFMLASFVPCVIMAACPRAQTVEMRLTGPGEERRRRKPFSLMPYGAALSIYATGLFSLATEGLSLRSWGLVIGSLAVTLLVLGRQIAAFLDNAMLLRRLGEQERRFRSLIEHSSDLTVITDRRGLVTFSSPAVTRMLGYAPEELLGSPMQALVHPDDLPAPAGGPWQGRVRHHDGTWRYLDVMATDRYDDPSVSGLISNARDITEARAFQEQLRFDATHDMLTGLANRALFHRGLDEADGDDLIGVLAIDLDDFKIINDTLGHHIGDRVLTVAAERLRACVREDTDLVARLGGDEFAVILRGTTAGEASATAGRIVAALMEPATIEGNTLAVKASVGVATGPARTAQLLLRAADTAMYDAKRSGKGTLAIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0T5</accession>
    <name>U5W0T5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21735"/>
    <dbReference type="EnsemblBacteria" id="AGZ42617">
        <property type="protein sequence ID" value="AGZ42617"/>
        <property type="gene ID" value="AFR_21735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4404"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="19482" checksum="A67DD79B48562F23" modified="2014-01-22" version="1">MSGPRLLASAATPGLRRAVFGGDDDLDEGGLRAALALTTDLGGRADVWFSAPSQAALQTARALGHDDPVVEHALADPDYGQWTGLGLGDIAGTDPEGLQSWLTDPEATPHGGESLAAVRQRAGAWLDKQAEQRIVAVAHPVVVRAALAHALGLSDDGVWQLDVSPLSLIRLTHRADRWHVSFPPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W105</accession>
    <name>U5W105_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Oxygen sensor histidine kinase NreB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Nitrogen regulation protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_16785</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Member of the two-component regulatory system NreB/NreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxygen, probably at the conserved histidine residue, and transfers its phosphate group probably to a conserved aspartate residue of NreC. NreB/NreC activates the expression of the nitrate (narGHJI) and nitrite (nir) reductase operons, as well as the putative nitrate transporter gene narT.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361743.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16785"/>
    <dbReference type="EnsemblBacteria" id="AGZ41635">
        <property type="protein sequence ID" value="AGZ41635"/>
        <property type="gene ID" value="AFR_16785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3404"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_13_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="289"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ41635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="373" mass="40261" checksum="6691B8C16ED39C73" modified="2014-01-22" version="1">MTACDRAELREVSEAVLAVTARLSVRDVLQTIVASARRLLDARYAALGVPGPADTFAEFLTDGISDEQWRAIGPLPRRHGLLGAMLRDPEPVRLTDVRSHPRFGWWPAAHPELTDFLGVPILHGDEILGSIFLANKTRPGGFTADDEELLRLLAAHAAIALVNARLYEQSRELSIVAERNRIARELHDAVAQKLFSLRLTADAAATLVTRDATRALAELDTVRRLAAEATEELRAIVVGLRPAELAGDGLDVALRKQADLLDRVHAPAVRFAGAAVPRLPAVREEAAFRIAQEALHNALRHAGPGVVTVTLRAAGGSVVLEVGDDGRGFDPREASRQFGLASMRERARGAGGRLDVLSRPGEGTTVRLEVPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W189</accession>
    <name>U5W189_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361924.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17235"/>
    <dbReference type="EnsemblBacteria" id="AGZ41725">
        <property type="protein sequence ID" value="AGZ41725"/>
        <property type="gene ID" value="AFR_17235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3495"/>
    <dbReference type="HOGENOM" id="CLU_109315_0_0_11"/>
    <dbReference type="OrthoDB" id="1494389at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="20670" checksum="73B3355D6B8873F0" modified="2014-01-22" version="1">MGTNPRIKPVERATDRTWDEWLTFMDGIDARNLDHQAIALKVYEELQGKVDPLGWWTQAVTVAYEQHIGRRIPGQRPDGTFQTSVSKATKLGMQDLMDRWVTFAATDPDVQKVIATDPRVSGTDRRITWRVKAADDSFITITSEPKPTGTATIVAGQQGLQTPELNLEAKQFWAATLTRFLASVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1M3</accession>
    <name>U5W1M3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative multi-sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_24175</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363693.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24175"/>
    <dbReference type="EnsemblBacteria" id="AGZ43103">
        <property type="protein sequence ID" value="AGZ43103"/>
        <property type="gene ID" value="AFR_24175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4897"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_51_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="11"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="127"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="198"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="246"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="409"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="652"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="768" mass="82265" checksum="4BDB6127F93705E0" modified="2014-01-22" version="1">MDSLDSLTGLLVDEAPDAVIICRSDGTIALANRRAHGMFGFPAGDLLGRNVDELVPDEIRKIHPGYRAGYLNSEHPPLRRLPLRGRRPDGSVFPVEVSLAAVAAPDGDTLVTAVLRDDTVHREAQEARALLASIIQSSHDAIVTTDLTGVVLTWNPGAEHLYGRTSAEMLGRPVDPIIPAERRVDEEEVRNLVRMGGRMDRYRTYRLHADGTTLAVSLLVSPLRDLDGTIVGMTTTARDVSDRERAEAQVQAVLDAAPDALLGVDDAGHVVLVNAEAERMFGYPRHDLTQMAVRRLLPDDLPAASAVLRTGGDSTPLDSERLTGAAGVPDWATRTARRRDGSELPVDISCSALDTGNGLIVVAAVRDITDRLAAQVEQESLREEAEQQKLEARFQRTQRLESLGQLAGGVAHDFNNLLAVILNYASFIVEDGRDTAFAKDAEQIARAARRGSDLTHQLLSFARREVIRPRALDVDTVVTEVHQMLQRSLGEHIALTIDTATDLPAVMADPGQLEQVLVNLAVNARDAMPGGGNLTIDTAAVEVDTEHAAGRSGLAAGSYVRIRVSDTGTGMPKDVIDKAFDPFFTTKPNGQGTGLGLATVYGIVTQAGGTVQIYSETGLGTTITVLLPVTDAEVRVPGPAEPAGTFSGQGTTVLVVEDETALREVTERILVRAGYTVLTAANGTEALKISGDRPVDILLTDVVMPGMLGKDIAEAIVAARPETRVLFMSGYAQPVLTTHGTLAADVHLLEKPFTSAELFKALHEQLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1P2</accession>
    <name>U5W1P2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative glutamate--cysteine ligase 2</fullName>
            <ecNumber evidence="2">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Gamma-glutamylcysteine synthetase 2</fullName>
            <shortName evidence="2">GCS 2</shortName>
            <shortName evidence="2">Gamma-GCS 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363718.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24310"/>
    <dbReference type="EnsemblBacteria" id="AGZ43128">
        <property type="protein sequence ID" value="AGZ43128"/>
        <property type="gene ID" value="AFR_24310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4924"/>
    <dbReference type="eggNOG" id="COG2170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044848_0_0_11"/>
    <dbReference type="OrthoDB" id="991285at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042398">
        <property type="term" value="P:cellular modified amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01609">
        <property type="entry name" value="Glu_cys_ligase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011793">
        <property type="entry name" value="YbdK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02050">
        <property type="entry name" value="gshA_cyan_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="39098" checksum="507D53D4607C1F57" modified="2014-01-22" version="1">MTGHTLGVEEEFLLLDPHTGENAPLSGKVLGALPDPVRDQSRLEFRHSMVEMVTPVCTGLAELREHLIGLRRAAADAAAQAGARLVAIGATPVAEPCREPTESPRFHEIARHYGPIAYDPAVCGCHVHVGVPGRDLAIEVCNHLREWLPVVQALSANSPLYQGADTGYASWRSVQLLRWPSLGPTPFAASAADHDRAVAQLVDSGVMLDESMVLWYARPSATFPTVEVRVGDVGTTVDDTVLVAGLIRGLVTAVRDDIAAGRAAVRVPDDLLRAAHWNAARTGLDGTLTDVRSQRSRPAWDLVGDLVDFVRPALKRLGDLDVVEAQLDRVRREGTGAARQREILRAGGSTGVMLDRLAELTISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W223</accession>
    <name>U5W223_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid adenylation domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18540</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18540"/>
    <dbReference type="EnsemblBacteria" id="AGZ41986">
        <property type="protein sequence ID" value="AGZ41986"/>
        <property type="gene ID" value="AFR_18540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3763"/>
    <dbReference type="eggNOG" id="COG1020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_224206_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensatn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010060">
        <property type="entry name" value="NRPS_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01720">
        <property type="entry name" value="NRPS-para261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="937"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="1489"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="2559"/>
            <end position="2633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1778"/>
            <end position="1799"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="972"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="1524"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="2594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="3143" mass="334965" checksum="49B2CF55BA2D1700" modified="2014-01-22" version="1">MTDVSVDRRPVSAAQLGIWVAQQVLPDSPLYNCGCYYEIGAADPVLLDRAVRHAVAETEALRSRFETIDDELWQLVGPAEPEPLEVVDLSAEADPQEAAQSWMRAAMAGVRPLGRGPLSRQAVLLLGADRRLWFHGYHHAVLDGFGQSVYASRVAEVYAALAAGQAPPPRVFTTLDEVHADAAVDPSTRRYAADRDYWLNTFADRPEPAGLAGRSAGAVPTQLRRIRALTPETATRFAAAAGAVGSTWPAAVIAAVAAYYHRLTGREDIVFALPLAGRRGRTSLTTPGALVNVLPIRLTVSSRDTFAELAGQAARRLADVLRHQRFRGEQLFQELGLTGERAFWGPTVNVMGFGGDLALGELTAVPHPLATGPVQDLKINFYGTPATGVRLEFDADPALYDAAAVEAHQTRLVRLLTALGDDPATRIGAVDLLDGTERHHLLHTWNETAPLHTGHYLPELFAEQVARTPDAVAVRQAGTALTYAELDERANRFARYLIGRGAGPERLVGVHLPRSADLMVVLLGVLKAGAGYLPIDASYPPDRIAAVLDDARPVLLVDDTAVLAASAGLPGTPVTDAERRTPLLPQHPAYVIYTSGSTGRPKGVLIDHRALGEFLTTCRDRYPQAAGTALLHSSISFDLTVTVLFTPLVAGGCVDVADLPNDGRPPAFVKATPSHLALLEGPGDNASPTGALVLGGEQLLGEALAPWRARHPQAAVFNDYGPTETTVNCADYLLEPGDDTPAGAVPIGRPLPGNRLYVLDPALQPVPAGATGELYIGGTGLARGYVNRPGLTGQRFVADPYAGPGARMYRSGDLARLRADGNLEYLGRIDDQVKIRGYRVEPGEIEAVLASADQVARCAVVVREDRPGDQRLVAYVVAADGTVDPSALTAHLAARLPAYMVPSAIVGLDELPWTANGKVDRRALPVPPQQAPVAGRAPASPRQEILAGLFAETLGVPRVGADDDFFALGGHSLLATRLVSRVRSVFGAELTIGQVFRSPTVAGLAAGLDRAGAGRSPITARPRPGRVPLSFAQQRLRFLDLLEQDSTAYHAPGALRLTGPLDRTALAAALNDLVVRHESLRTVFAADDEGFHQVILTPEQAPVPLPVAELDEAALPDRLAAEAAQPFDLAGEPAIRARLFVLGPQDHVLLLVLHHITGDGWSMRPLARDLAAAYRARLTGGEPSWPALPVQYADYTLWQHEVLDTEGEQLGYWRQNLAGLPAELALPVDRARHDDMPAHAERASADLPAELHESLHAVAREHGASLFMVVQSALAALLTRLGAGEDIPLGTPIAGRSDAAVEDLVGFFANTLVIRADTTGDPTFGELLGRVRETSLSAYAHQDVPFERLVEELNPPRSLARHPLFQVCLTLHHADPATVVAEVTDLPGVAVRMLPVPGADAMFDLNLELTERFTPDGRPAGVRVDLDAAAGLFDPATVRALTDGFAAVLTSVAADARQRLSEVDVPALGTRPGLTPAEPVVVPAVRSRAPRTPQEQILCTLFAETLDTDAIGVDDDFFARGGHSLSAVRLLSRIRAELGAEVSIRTLFAHPTVTGLLPHLSTATRPAIETAARPARLPLSYAQQRLWFLHHLEGPSATYNIPVALRLTGDLDRSALTAALSDLVLRHESLRTVFAQDESGSYQVVLPGERARPELIVRALPSAGREEAVREAAAEPFDLGRELPVRVTLFEGTGEHVLLVVLHHIAGDGQSMGPLARDLTTAYAARAAGSAPAWAPLPVQYADYTLWQRTMLGAEDNPGSEFGRQLGYWRETLAGLPGELQLPTDRPRPPRQSYRGDRVSRGVPAELHRALRELARETRSSLVMVVQAALATTLSRLGAGTDVPIGMPIAGRTDAALEDLVGFFVNTLVLRNDVSGEPSFRDLVARVRETNLGAYAHQDVPFERLVDVLSPARSLARHPLFQVLLGFDDNTEALSALRLPGLAVEAGAPETGRAKFDLSFFFDEAYAPDGTPQSLTAALEYSTDLYDRSTAEAMLATLLRVCAGVTADPDVPVSRVDVLDDADRDRVLRHWNRAPHPVAADLVPDRLARQAAARPGELAVLADSAELTYGELDAAANRLARHLIGLGAGPEQVVAIALPRTPGMVVAMVAALKTGAAYLTLDPAVPDQRLRAVVADCGAVAVVTDTAIAPRLDGTSRAVVLDDPATAAAVTGQPGTAITDADRRGPLDPRHPAYVVYTSGSTGTPKGVVMPMGSLTNLLAWHTGTYPAAPGTRTAQFLAVSFDFAVQEILQALVAGKTLVLPAEHVRHDAYELAAWIDRYAVNELFAPRLVIDAVLAAAADRGSDLRTLTDVFQGGEAFQLGDELRAWAAGGNRRAHNVYGPAETHAVTTATMPADPAAWPATAPIGRPLWNATVFVLDARLQPVPLGAPGELYVGGAQLARGYLNRPGRTAERFVASPFGAPGDRLYRTGDLVRWTRDGELQFLGRGDHQVKIGGFRVEPGEVEGVLAAHPGVTTAVVVPRDDLPGGTRLVAYAVPDPQHDLGPDGGRRLRAHLEEHLPSYLVPAAVVLLDVLPLTPNGKLDRAALPAPEFGGSTGRGPRDDREKLLAALFAEVLGVAEVGVDEGFFDLGGDSIMSIQLVSRARRAGLELSVREVFEHRTVEALAPVVREVGTAPAEAPGEAYGDVPATPIMHWLADRVTTAAVVDTFNMSIVLQVPATLAVAPLTTAVQAVVDRHDALRARLHTEGTWSLTVPEPGSVDATTLVSRVDATDLDEPALNTLVRQHGHAARSRLDPARGVQLQLVWFDRGPSTAGLLLVLVHHIVMDGVSWRVLLPDLMEAYSAAEAGTEVRLQPVGTSLRRWAHGLVAAAGDPLRAAEAGWWRDVLRHSDPLLGARPLDPAVDTYGTAGSLTRHLDPVVTEAVLTRVPALFHAEINDVLLTAFVLAWSRWRGDRTTGLLLDLEGHGREEHLVPGADLSRTIGWFTAIFPVRLDAGPVDLADAFAGGPAAGAVLKRVKEQLRAIPDKGLGHGLARHLDPGAAPGFAGTAAPQAGFNYLGRFAAPAPGSAGWTPVFGLEAVPGEEDGLPLAHTVDLNARTEETADGPSLTAMWTWAGELLTTAQVDELAGLWFEALEALVAHADGPGAGGLTPSDVPLAVTQDEIDAFEDEFQDEFDAEEEDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W260</accession>
    <name>U5W260_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endoglucanase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_24740</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363804.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24740"/>
    <dbReference type="EnsemblBacteria" id="AGZ43214">
        <property type="protein sequence ID" value="AGZ43214"/>
        <property type="gene ID" value="AFR_24740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5012"/>
    <dbReference type="eggNOG" id="COG2730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020735_1_0_11"/>
    <dbReference type="OrthoDB" id="1395441at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666011" description="Endoglucanase" evidence="5">
        <location>
            <begin position="34"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="6">
        <location>
            <begin position="461"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="563" mass="61068" checksum="A770755E96FCF092" modified="2014-01-22" version="1">MFSTRNKRRLAAVAAAVLAAPLLWLATSGTSQAAQAAVPAKDWLHVQGNQIVDEAGDPVWLTGTNWFGFNAGERVFHGLWSANLTEVTKSMADRGINLVRVPISTQLLLEWKAGQATVPSGVNTYANPELVGKTSLEVFDTFLALCERYGMKVLLDVHSAEADNSGHVYPVWYKGTVTPELFYQAWEWVATRYKTNDTLVAMDVKNEPHGKQSESPRAKWDSSTDVDNFKNTCQVAGRRILAINPNVLILCEGIEIYPKDGVTWSSTDEKAYDNVWWGGNLRGVRDHPVDLGANQDQLVYSPHDYGPLVYEQPWFQKPFDKATLTADVWTPNWLYVHDSGTAPLLVGEWGGRLGQDARQDRWMTALRDLIVEKHLHQTFWVLNPNSGDTGGLLLDDWKTWDEQKYALLKPALWQYAGKFVSLDHQVPLGGAGSTTGISLAARYGGGADPSTPPVSTPPTSTPPATGACSATYTQTGTWNGGFQGEVTIKNTGTTAGRTWTVTWTYPSGTSVSSLWNGVLSTSGSAVTVRNAAHNGSLPAGATATFGFVGSGPASTPTITCSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2S1</accession>
    <name>U5W2S1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydropteroate synthase</fullName>
            <shortName evidence="1">DHPS</shortName>
            <ecNumber evidence="1">2.5.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dihydropteroate pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DHPS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20040"/>
    <dbReference type="EnsemblBacteria" id="AGZ42280">
        <property type="protein sequence ID" value="AGZ42280"/>
        <property type="gene ID" value="AFR_20040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4066"/>
    <dbReference type="eggNOG" id="COG0294">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008023_0_0_11"/>
    <dbReference type="OrthoDB" id="1275854at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00156"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="PTHR20941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="2">
        <location>
            <begin position="26"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="31500" checksum="CE4A2DF8B662C264" modified="2014-01-22" version="1">MSDYAPPLISADRVIGGRAYDFSRRVAVMAIVNRTPDSFHDKGRTFALTKALDAVRAAADAGADWIDIGGVPFAPGPDVSPAEELDRVLPVVEAARDLAVVSVDTYRPEVAREVIKAGAGVVNDTSGLRDPEMADVVAGTDASLVITHSLAAPRTLHPSPRYDDVTAEVGAFLRQRVELALARGVRPDQIIIDPGHDLNKNTYHSLELTRRLREITAIGYPTLAALSNKDFIGETLDRPQQERLVGTVAAVVFCVLQGARIVRVHDVAAAVDAVRMTEAMLGLRGPVHARHNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W321</accession>
    <name>U5W321_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_25740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364089.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43412">
        <property type="protein sequence ID" value="AGZ43412"/>
        <property type="gene ID" value="AFR_25740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5218"/>
    <dbReference type="eggNOG" id="ENOG50338VG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_690619_0_0_11"/>
    <dbReference type="OrthoDB" id="1546099at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SSF160631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="395" mass="43042" checksum="DB84A677CEC88B49" modified="2014-01-22" version="1">MNHRDDRGPIHDRVLDALAAETGASPRLGPGHDWAAVEASLGGLRLPADYKRLVDAFPHGEFGGLVRVVRPGVPDGPESAYPEYYLRLLDDLREQREIEWGIYPHPLYPERGGLLPWGDTLSGQLIFWLTEGDDPDAWPVVWTDRGFEEWQLRRRSMSEFLLELITDPAVERPGLPAGGSGFRAFGGGATAAPIGLLAGHAVPAGSSADQEAPIGFSADHAEPEDAVDGLLEALGTMPIEEPTLEWFVRAGEWGFMFPADYRTFHDTLGPGLFCDIGIFGPGAPEGWNAADLLRAGRAAAEAAGLDHLRFHPDPGGLVPWGMTQDGWLLGWAARHPDPDLWCVAALGPGFVRIDWPELSFSEFLLFHAGIRDGYDVALPDRPRWSGPPTFRGLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5W368</accession>
    <name>U5W368_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_26960</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5 8">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364564.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26960"/>
    <dbReference type="EnsemblBacteria" id="AGZ43653">
        <property type="protein sequence ID" value="AGZ43653"/>
        <property type="gene ID" value="AFR_26960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5463"/>
    <dbReference type="eggNOG" id="COG0372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025068_0_0_11"/>
    <dbReference type="OrthoDB" id="1412360at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00717"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004108">
        <property type="term" value="F:citrate (Si)-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06114">
        <property type="entry name" value="EcCS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024176">
        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010953">
        <property type="entry name" value="Citrate_synthase_typ-I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01798">
        <property type="entry name" value="cit_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001369-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="427" mass="46847" checksum="C5C315E312EEE584" modified="2014-01-22" version="1">MTDVKLDHPGGQLSMPVREAVEGPGGIDVSALLKETGYVTLDQGFVNTASCASAITYIDGDAGILRYRGFPIEQLAGKASFLEVSYLLIHDKLPTPAELAEFGDKIRVHTLLQEEMRAFFSGFPRDAHPMAVLSSAVTALSTFYQDALDPADPEQVEISGIRLMAKLPTIAAYAYKKSIGHPLPYPDNSLDYVENFLRMTFGLPTVNYEVDPTLAKVLDMLFVLHADHEQNCSTSTVRLVGSAQANLFASVSSGINALSGPLHGGANSAVLEMLEGIRADGGDVDAFVRRVKAKEKGVKLMGFGHRVYKNYDPRAAIVKKAVQDVISTMDRPDPLLEIALKLEEIALADDYFVSRKLYPNVDFYTGLIYKAMGFPTKMFTVLFALGRLPGWIAQWGEMMADPTTKIGRPRQLYTGEAERQFVPITER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3D4</accession>
    <name>U5W3D4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chromosome condensation regulator RCC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_27285</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560056.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27285"/>
    <dbReference type="EnsemblBacteria" id="AGZ43718">
        <property type="protein sequence ID" value="AGZ43718"/>
        <property type="gene ID" value="AFR_27285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5529"/>
    <dbReference type="eggNOG" id="COG5184">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_505914_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="SSF50985"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666340" evidence="4">
        <location>
            <begin position="24"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="427"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="560" mass="55532" checksum="BAE445CC882E6919" modified="2014-01-22" version="1">MWIFIRLAAAAALLPLFPQPASAAPVPDPTITRATPAPEPAVVRAAQAAILERAGLAPGPSAQESGVGAGVVAAGPRHTCAIVQFADLWCWGANPDGRLGDGTTRSSAAPVRVSAKGGLAGTAGILNVQAGRAHTCALTLESAEAGLVAYCWGANDEGQLGDGSFDPRPRPAQVAAGVVQIVTGADHTCVLTVELTVSCWGRNNAGQLGAGTLGTSEANPQEVPGLTGVIDLAAGDDNTCALDQTGKAWCWGAGSHGRLGDGGGVSADPSPVPVAVAGGPYGEISLGRRHACALGTGSAVYCWGADDHGQAGGRGDRDVPTRNGQRTYTGVRAGGDSTCGVTSAGEAFCWGANGDGQLGTGDLADRARPAAIDRSRMRTSSLARLLLGSGSPRVADLSVGAHGSCGVDVSMSVYCTRSGLLTAVPLAPGSVTGVRVAAQPGALRVSWTAPEETGADRIGAYVALAFVGAGLDDSRSCTANPALTCTIPGLAGGEQYTVLVAAVSRGGISYSAVAHGVPAGAGSGGGLPVTGPGPAAALGLVLLTLGWSLRRASRSVGCRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Q3</accession>
    <name>U5W3Q3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative FGGY-family carbohydrate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_26930</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364551.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26930"/>
    <dbReference type="EnsemblBacteria" id="AGZ43647">
        <property type="protein sequence ID" value="AGZ43647"/>
        <property type="gene ID" value="AFR_26930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5457"/>
    <dbReference type="eggNOG" id="COG1070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_3_2_11"/>
    <dbReference type="OrthoDB" id="1619686at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="2">
        <location>
            <begin position="4"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="3">
        <location>
            <begin position="256"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="523" mass="54228" checksum="DBA7FAB7748CF0BD" modified="2014-01-22" version="1">MDVVIGIDTGTTATKAIAAGPAGEVRAMTSVHYPLSVPGPGQAELDPDKLRDAAIEALVTVAKESAAQGDRVIAIGLSAFLHGLAPMDANGAPLGPLITWADNRASGQADEINAAGRAKGLQARTGTPVHPMSPLAKLTWWNANDPAKVRDTPRWGGVKEVVLAGLAAEGFLLDLSLASGTGMYDIHERHWDPEALSLTGVRENQLAEVVPTTHRLRLRPEVASAAGLPPEIPLIIGAADGPLANLGVGATPAGVAAVSLGTSGALRTIVNGPTADNAGRLFCYALTEEHWVVGGAINNAGSVVRWAGETFAGSFDRPAAEGEDADVRDAALLVEAAEIAAGSEGLLCLPYLLGERAPWWRGGMRGAYLGLRREHGRAHLVRAAVEGVCQQLALVRDTFADEGIAMTEVRATGGAVASELWVGILASALDLPVAVAETPEGTALGACLLARHALGELPDLDQAAAMIGINDHTRPDESDAALYRRLRPLVERSALAVTDVLTELDKIAPEPLPGTEKAVPNPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3S6</accession>
    <name>U5W3S6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction protein containing a membrane domain an EAL and a GGDEF domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_27055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560010.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27055"/>
    <dbReference type="EnsemblBacteria" id="AGZ43672">
        <property type="protein sequence ID" value="AGZ43672"/>
        <property type="gene ID" value="AFR_27055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5483"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_49_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="485"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="625"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="886" mass="94867" checksum="4A9C842154999CEF" modified="2014-01-22" version="1">MGAVDVDRPLVGGLAVGRPQLVGCHLERAQLVRADLDGTVLERAELVRGRLDRGAVAVTGRRRRLHPRLAVGMLTVAMGVASAAVAFLLAGQGFFAFALNPGWLAGLAVLFFVTEGLTVHVRVRRGAHGINVSEIPMVGGLLAFDPVTVIVVRALAGGAGILVLRRQRGQKLAFNVALLAVQASVGGLVFHTIAPRALGTTVHGLDPRDWLATYAAMISADVVAAVLLTAVIAVHDDPGEWRRLPAALRSTWVVVVTTTVAMISFLAVVEARWALALVGIVIVVLFLAYRAYVALGDGNAEVEQLYAFTRALDGARGIDELVEVVLDQARDVLRAGTAELVVAHGTELRHTRLDGLSGRVTATLGRDDDWLRPALLGSPVLRPEEAGGEAMAVPVAFGGTRGVLVVTDSLADAGGFSEAKLRLFEALANHAGAALGRADLVDRLRREATEKEHLALHDTLSGLPNRRYFHELLDAELATAREQGTGVAVLVLDLDRFKEINDALGHETGDALLREVGERLRGHLDGRGRVARLGGDEFAVLLATDGTAEAALAVAAQVAEVVERPILLGPLTLAARASVGVASAPLHGDDSQTLTRHADVAMYAAKDSNGLRLYDSELDTNSPERLALIADLRRAVERRELEVAYQPKVDPRTGQVVGAEALARWRHPERGPIPPDVFIPLAEHSGLVWPLTLHVLELALRRRADWARAGHDLHVAVNLSPNSLLDAALPELVARLLAETGNPAHALTLEITESTILADPAGSRATLDRLHALGVQLSIDDFGTGYSSLGRLRELPIHEVKIDKSFVQRMAVDHRDRAVVRSAVQLGHALDMRVVAEGVEDADTYAYLADEGCDVVQGYLLSRPLPPEEFSSWMNARSRNVTHTYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3V8</accession>
    <name>U5W3V8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_27230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27230"/>
    <dbReference type="EnsemblBacteria" id="AGZ43707">
        <property type="protein sequence ID" value="AGZ43707"/>
        <property type="gene ID" value="AFR_27230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5518"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_135_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="333"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="364"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="585" mass="62099" checksum="F642325CD99115E6" modified="2014-01-22" version="1">MDTAYRVSPLRRGDPERLGEYWLTGRLGSGGMGVVYLATDRDDNYVAIKLVHAALSGDPEFRGRFRSEVDRARQVPSFCTAEVLDADLDHNPPYLVVEYVDGPSLAEVVEERGPLRAAALNSLAVGVATALTGIHGAGVIHRDLKPDNVLLAPGSPKVIDFGIARAFEATSQHTRTDQMVGTVAYMAPERFSSEPGTPLTAAADVFAWGCVVAYAGTGMTPFHGDSPPATAARILTQPPHLGGLPEPLRGLVELSLSKNPEDRPTARELLDMLLGDRPPRRTPVFSPPPDFPYEEPAPVPAGRGRSHRLLAALAVLLVLAGVATVALVLNNHGGVGNPLNTGATRGGGTAAGAPESPADSSTGAPAPETPPPTAPADYTEPAAEPAEEPEEESSPVPVEPTGGEPIIQDPLNQPGQWLDSEIREQNANCFTRGVMKAGRVDRGTYQCVGPDERIEDDFGVEVTTALQSAGSCAAIWFHWDPKAGGQVLRVCQDEMSIAADKADDRRVYGRIKLGNRIALRKSTRIHLVVREGEAQVFRGGKFAGSVPLPDSGPDEGQVLLGLSVEAVDTDPPYTVTFANVDIRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W416</accession>
    <name>U5W416_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycosyl hydrolase family 98 putative carbohydrate binding module</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28485</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560295.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28485"/>
    <dbReference type="EnsemblBacteria" id="AGZ43958">
        <property type="protein sequence ID" value="AGZ43958"/>
        <property type="gene ID" value="AFR_28485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5775"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011166_1_1_11"/>
    <dbReference type="OrthoDB" id="469334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029483">
        <property type="entry name" value="GH97_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019563">
        <property type="entry name" value="GH97_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029486">
        <property type="entry name" value="GH97_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14509">
        <property type="entry name" value="GH97_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14508">
        <property type="entry name" value="GH97_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10566">
        <property type="entry name" value="Glyco_hydro_97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665521" evidence="3">
        <location>
            <begin position="25"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="4">
        <location>
            <begin position="607"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="735" mass="78238" checksum="462828C338E1A104" modified="2014-01-22" version="1">MLRCALASLLSAAVVVVTPAPASAATTWTVTGPSTGSPTAAQVTLNDNGTLSFGVSSQGRAVLSPSAIGIETSTADLTRNLTFTGRSDRTVTEAYTMTTGKQRSRSTVYSETTLSFTGTGGARLDVVVRASDTGAAYRYVLPGSGSVTVRREATSWTVPASSNAWLLPAHPEDQGYPVQTTAGAAASGTFRVPALFESGGAFALVAETNLDGRYAAASLTHSSGSGTYGTVLDGAITTTLPLATPWRTAAVGSLASVTQSTLVDDLSAPSRIANPSWVRPDTVAWSWLTEHASPSSESRQRQYIDFAARNGWGYVLIDEGWQESWAPGVIAYAKSRGVQVILWFNSSGLWTAAQRETWLPRIKSWGAAGIKVDFSYEFTQPTLQWYDAILARTAELQIMINFHGSATPRGMQRTWPHVLTAEAVHGAEQYLRAAKNTILPFNRNVIASMDYTPVIFSPFNRDTTPAHELATAIVFESGWQHLGDNPESYESRPEALRILNQLPTTWDESRLLGGRPGQDAYFARRNAGRWYLGGISAQSARTYATPLSFLGSGQWLLETVRDGSNSSLVRETSVVTSTSTLSVAMLERGGFASVACPYTAGLTTCGTPISVLRGQASGRCVDVPFSTEVNRTRVELFDCHGSANQTWTATAARELKVYGTKCLDVDGGGTADGTPVTIFDCTGGTNQQWNVTTDGAVVSVASGKCLDAVGAATANGTQLQIWPCTGGGNQRWSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4L3</accession>
    <name>U5W4L3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_23030</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363387.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23030"/>
    <dbReference type="EnsemblBacteria" id="AGZ42876">
        <property type="protein sequence ID" value="AGZ42876"/>
        <property type="gene ID" value="AFR_23030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4667"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_11"/>
    <dbReference type="OrthoDB" id="1108236at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665682" evidence="6">
        <location>
            <begin position="28"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="12"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="490" mass="49201" checksum="4DC331982A19C82C" modified="2014-01-22" version="1">MIRLRSRTGAALITATVLASMAGFLDASVVNVAIPAIGRDLQAGVVALQWTVAGYLLTAAALLLVAGALADRFGRRRVLIAGLAVMLVASVLCSAASSAGELIAARVLQGVGAALVVPSSLALLNGTLSGADRAPGIGIWAGLSSLGGLLIGPFAGGWLVDHVSWRAIFLLNIPLILAALLALIPVPEAAHPTEGRLSIDTAGALLAVLGLGGLIDALTTGSVEGWTSPRVIAGLTVGIVCLAALVPVERRVRQPMLKLSLFGSRQFSAINLATVLFYGALAAAGYLLVLRCELTLGWSATRAGSVQIPASAVFLALSPVSGVLVRRVGPRLLMTAGIAAVGLAFLWLAIVRGGYAGAILPAVLLWGVGLGLTVTPLTAAVLAAVGDGDLGEASAISDVASRLGGAVLTALVPVLIGVQAGRGLGPALTDGYRPAMIVLAALCGVAAVIAAVYVRAGQPVASSPIRFAPPAPFHGCAPVRTVVRPSEVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4L9</accession>
    <name>U5W4L9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_23055</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363392.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23055"/>
    <dbReference type="EnsemblBacteria" id="AGZ42881">
        <property type="protein sequence ID" value="AGZ42881"/>
        <property type="gene ID" value="AFR_23055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4672"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019414_0_1_11"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EHN" evidence="4">
        <location>
            <begin position="13"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="405" mass="44361" checksum="52AC0EB4E586AF41" modified="2014-01-22" version="1">MTTQQQQPASRAIRPFTVRFREGALDDLRRRLAATLRPTRELVGDRSQGVQLATMEALGQYWEKEYDLRRVQSRLNAVPQFTTVIDGVDIHFLHIRSRHPDALPLIMTHGWPGSVIEMLDSVGPLTDPTAHGGSAADAFHLVLPSLPGYGFSAEPVELGWDLVRTARAWAELMDRLGYDRYVAQGGDVGAGVTDAMGRLGPQGLAGIHTNLLVTALNDPASLPNTTEEEQAALAALKVFQTSGNGYFVEMATRPQTIGYALLDSPSALAAWMIDHDTDAYYKIASAFVDGKPSGNLTADHILDNITAYWLTGTGASTSRSYWEAYGPDAPAAGRPPLPDPRVPVGFSTFPGEIWRTPRSWAEQSYPTLNYYGVAERGGHFAAWEEPELFAEQVRAAFRPLRGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4P1</accession>
    <name>U5W4P1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560510.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29560"/>
    <dbReference type="EnsemblBacteria" id="AGZ44173">
        <property type="protein sequence ID" value="AGZ44173"/>
        <property type="gene ID" value="AFR_29560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5992"/>
    <dbReference type="HOGENOM" id="CLU_705208_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="391" mass="42480" checksum="6A31C240DDCCE982" modified="2014-01-22" version="1">MTVRFEFQAARQTETKATVVVQESLIGLDVWPDVPMVRKFEEDLTAIDRLVLEAAIALNPVHAADIEEITGIPAEAVDGIISRLVSLGLLQTDGYGAVATDYAQPALDRAAVVRLRQTPVTLLYLADTDEIVALSSSEGRRAPRLHHTKPAGFVDVQRGASLTSQHNLIAERIAGQAIRGLSQDIVSPVVTETEIADVCKQYRCSGYVRIGSDGTFRAYLKIEVGPSKRLTLQIPYANQLASRWEDLAEQAVSAADAWGPVVATRADGSWRYELGQEAAASAAAARIRLGHPGGLLIHGERDVVYVDVEFVPGDERASHIFALQHAVAEVSARSVANIDSEELAAITTASTERYDLPPNGLTVAEVRSALWTGQHYQHVYALRRQEDFPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4R7</accession>
    <name>U5W4R7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363480.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23305"/>
    <dbReference type="EnsemblBacteria" id="AGZ42931">
        <property type="protein sequence ID" value="AGZ42931"/>
        <property type="gene ID" value="AFR_23305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4723"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077127_0_0_11"/>
    <dbReference type="OrthoDB" id="1445278at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_7C" evidence="2">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="29589" checksum="D161D51E2563590A" modified="2014-01-22" version="1">MTAVITLCSASRLDHVRRQQRLLASAPVRRIIVWLDADAPPLSEFPGADMVHVPPGQLGLRLAAGRDRGAERALAAGEDLLVFLDADCLPGPDLLSRYRAAADAHPGAVLCGPVTYLPEGIRPENADALPALTAPHPARPAPPDHQNVVAGPDDHRLFWSLSFAVTAQTWRTVGGFDEAYEGYGGEDTDFGFTARARGVPLVWVGGAHAYHQYHPTSAPPWQHLDDILRNGKLFADRWGSWPMEGWLEAFAAAGAIERTPEGWQRTPVRAAGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5C6</accession>
    <name>U5W5C6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_30790</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560753.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30790"/>
    <dbReference type="EnsemblBacteria" id="AGZ44418">
        <property type="protein sequence ID" value="AGZ44418"/>
        <property type="gene ID" value="AFR_30790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6233"/>
    <dbReference type="eggNOG" id="COG3506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_534015_0_0_11"/>
    <dbReference type="OrthoDB" id="1489522at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="507" mass="51594" checksum="FD76FF6234A2A2E6" modified="2014-01-22" version="1">MIKLLHAEWTKLRTVRGWMIALVVAAAAVVGLGLLAGGQGSCGRNGPGSECVLPVGPGGEEVVDRFGLVHRPLTGDGTLTVRVRALTGLLPPRPDGREEKEGGSPRQAGPGAGGEPGLAPWAKAGLIIKDGTSAGSTYAAVMLTGAHGVRMQHDFVHDRAGADRAEWLRLTRSGPAITGEQSPDGTSWTTVGTVRLDGLPATAEVGLFATSPQHTWLSRNGVGMTDATGGPSFATGTFDSITLRGAWRNGPWTRTAVGGDDGSAGDGDPATGEVSVTGSGDIAPAVSGLAGLGTTISQTLTGTFAGLIVVVVLGAMVMTAEYRSGLVRTSFAAMPGRGRVVAAKAVVLGGTTFAVGTVAAVIVVVAGQRVLRANGVYLHPVSTAAEVRVIAGTGALLAGAALLSLALGTVLRRSLTAVTTGIVVIVLPYLLALTVLPTVAAEWLLRVTPAAAFALQGTAPRYPQVENFYLPANGYFPLPPWAGLAVLAAWTALALTAATIVLRRRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5C8</accession>
    <name>U5W5C8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioredoxin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363701.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24215"/>
    <dbReference type="EnsemblBacteria" id="AGZ43111">
        <property type="protein sequence ID" value="AGZ43111"/>
        <property type="gene ID" value="AFR_24215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4905"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_8_11"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="25"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="558" mass="58760" checksum="218D0244CEECA80E" modified="2014-01-22" version="1">MTSKAAQDPVDPRFSDSQLAGLRAYGEIRTVAAGDVVYSPADERNDLLVVLAGEIGVGDETPGHEVEFARFGASGFVGELNLLTGQRPYVTARATVESTLLAIPHDRLRELLDRETDLADTLVTAMIARRRLRLADGATNAPIEIIGTAQSEPSLALRTFLGRNTIPYRWADADADPGELASAGASRADLPVVVHPGGVLLSCTPGRLAEVLGLTQRPGDDRLYDVAVVGAGPSGLATAVYGASEGLEVAVFDATGPGGQAGASSRIENYLGFPDGISGAELTTRAAIQAQRFGARLNSPCRVTGIEPSPLGFTVTLGDDTRLSARTVVVATGAQYRRLPLDNWERLEGAGIYFAATDLEAGLCAGEPVLVLGGGNSAGQAALYLARRCTRVTIVIRRESLAESMSSYLIDRIEAHDRITVASSTVVEAVDGAEHLETVSLRRTTTGEVTRAEARGLFCFIGARPATGWLPATVRRDDDGFVLTDVALDGASRLPYETSLDGVFAAGDVRLGSMKRVAAAVGEGSSVIRSVHQFLSARTDRDPERDPSQGEPAPVLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5K1</accession>
    <name>U5W5K1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Phosphatidylinositol phosphate synthase</fullName>
            <shortName evidence="10">PIP synthase</shortName>
            <ecNumber evidence="10">2.7.8.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">CDP-diacylglycerol--D-myo-inositol-3-phosphate 3-phosphatidyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_31115</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conjugation of the 1'-hydroxyl group of D-myo-inositol-3-phosphate (also named L-myo-inositol-1-phosphate) with a lipid tail of cytidine diphosphate diacylglycerol (CDP-DAG), forming phosphatidylinositol phosphate (PIP) and CMP. PIP is a precursor of phosphatidylinositol (PI) which is an essential lipid required for cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 10">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + 1D-myo-inositol 3-phosphate = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61216"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:85356"/>
            <dbReference type="ChEBI" id="CHEBI:144472"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>1D-myo-inositol 3-phosphate + a CDP-1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60504"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Contains a di-nuclear catalytic Mg(2+) center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10 11">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560817.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31115"/>
    <dbReference type="EnsemblBacteria" id="AGZ44483">
        <property type="protein sequence ID" value="AGZ44483"/>
        <property type="gene ID" value="AFR_31115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6298"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080384_0_1_11"/>
    <dbReference type="OrthoDB" id="1232102at2"/>
    <dbReference type="UniPathway" id="UPA00220"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02241">
        <property type="entry name" value="PIP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044268">
        <property type="entry name" value="PIP_synthase_PgsA1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="CDP-DAG binding" evidence="10">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>a CDP-1,2-diacyl-sn-glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>a CDP-1,2-diacyl-sn-glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ44483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="21360" checksum="D41DEB13B0928A8E" modified="2014-01-22" version="1">MAKIVSVPARALVSYVLHPAARFLLRIGVTPNMVTVAGTVGVVIGALGFGARGELFWGTVVVTAFALTDALDGTMARMRGPSGKFGALLDSSMDRIADAAVFGAVVYYLAGLGNPYGGVVAALLSLALGQVVSYVKARAQSLGLDADVGIAERLERLLIVGVGGLLGAAGLEWGLPAALWVLAALSLVTVLQRLIHAAKSDVVPVADGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5R6</accession>
    <name>U5W5R6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AraC-family regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363937.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25345"/>
    <dbReference type="EnsemblBacteria" id="AGZ43335">
        <property type="protein sequence ID" value="AGZ43335"/>
        <property type="gene ID" value="AFR_25345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5136"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_88_4_11"/>
    <dbReference type="OrthoDB" id="1268974at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="190"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="31398" checksum="867BF4E35C253026" modified="2014-01-22" version="1">MDELSLLDTTGILRQPWIRPERTSAGLGWSGLYVSAQQEQPYRAAFDGARSHLVILHLDGPVTVRRGQAGLVSARTVGPGGFFVHPAGKDLTVELGGDLATVHVYLDDEALQAACDGPAVELAEELGVADPELERLVRALDQALRGWEPSGRTYVDELCGLLADHLVRRHSVRRPAGSPRAGGLSARQLARVRELLVERLGEPVPLADMAAVAGLSVSHFSRRFKAGTGLSPHQFLVRLRLETACRLLRARVMPIAAVASHCGFSHQEHLTRVMRRYLQTTPGALRRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W602</accession>
    <name>U5W602_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gamma-glutamyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31715"/>
    <dbReference type="MEROPS" id="T03.001"/>
    <dbReference type="EnsemblBacteria" id="AGZ44603">
        <property type="protein sequence ID" value="AGZ44603"/>
        <property type="gene ID" value="AFR_31715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6419"/>
    <dbReference type="eggNOG" id="COG0405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="521" mass="54521" checksum="08843ED67F67DD87" modified="2014-01-22" version="1">MTEPFSAGIGGGGFFVYYDAKHKKVHTIDGRESGPATMTEDIFIDPADGLPYDFQEARVSGLSVGTPGTLATWEAAAGKWGTRSLGSLLQPAAKVADKGFAVDNTFRQQIADNAAAFAQFGSTSELYLPAGAPPVVGSTQRNPDLADTYRLIAKRGTGVFYRGSVGADIVRTVQNPPVAGAPGAPWAYPIRPGDLRLSDLANYRLRFPAPTKSGYRGLDVYGMSTPSSGGTAVGEALNILEKTDLSKVSMTQALHRYAEASALSFADRNRYVGAYTPQSTLDKLISDGWATQRACQINPAKALTKPVPPGDINASGCTPAAVGGPVEDGQSTTNLTVADKWGNVVEYTLTIEQTGGNAMVVPGRGFLLNNEMTDFNFTGTQGTAPDPNLPGPNKRPRSSMSPTIVLKHGKPFLALGSPGGATIITTVLQVLINRVDLGMTLPEAMAAPRASQRNTAGIQAEPAFHTQYGPGLEALGHSFSADVPELGAATAIEFRPGGGMIASAEPVRRGSGAAGVVRVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W635</accession>
    <name>U5W635_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Holliday junction ATP-dependent DNA helicase RuvB</fullName>
            <ecNumber evidence="6">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">ruvB</name>
        <name evidence="9" type="ORF">AFR_31060</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms a complex with RuvA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560807.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31060"/>
    <dbReference type="EnsemblBacteria" id="AGZ44472">
        <property type="protein sequence ID" value="AGZ44472"/>
        <property type="gene ID" value="AFR_31060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6287"/>
    <dbReference type="eggNOG" id="COG2255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055599_1_0_11"/>
    <dbReference type="OrthoDB" id="874197at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00016">
        <property type="entry name" value="DNA_helic_RuvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041445">
        <property type="entry name" value="AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004605">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008823">
        <property type="entry name" value="RuvB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42848">
        <property type="entry name" value="PTHR42848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17864">
        <property type="entry name" value="AAA_lid_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05491">
        <property type="entry name" value="RuvB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00635">
        <property type="entry name" value="ruvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="AAA" evidence="8">
        <location>
            <begin position="52"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="320"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="60"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="37564" checksum="0C2FACE75CE733B3" modified="2014-01-22" version="1">MTDDLVSPFAGDEELDAEASVRPRRLADFIAQHRVRDQLELLLKGAMGRGTPPDHILLSGPPGLGKTTLANIVSAELGTGIRTTSGPVIERSGDLAAILTGLAEGDVLFIDEIHRIAKPAEELLYSAMEDFRVDVIVGKGPGATAIPLDVEPFTLVGATTRAGLLSGPMRDRFGFVAHLDFYSPADLDALLHRSARILGVPITPEGAAEIAGRSRGTPRIANRLLRRVRDYAEVRGNGIVDEATAKAALTVYDVDQLGLDRLDRAVLRALIESFRGGPVGLSTLAVAVGEQSDTVEEMCEPFLVRAGLLARTPRGRVATDAGWRHLGKKPPEGRGALPNQPDLFARDAETSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W638</accession>
    <name>U5W638_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PAP2_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_26030</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26030"/>
    <dbReference type="EnsemblBacteria" id="AGZ43470">
        <property type="protein sequence ID" value="AGZ43470"/>
        <property type="gene ID" value="AFR_26030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5277"/>
    <dbReference type="eggNOG" id="COG3266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056733_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="PAP2_3" evidence="7">
        <location>
            <begin position="46"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="231"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="29626" checksum="439F0532D7231A46" modified="2014-01-22" version="1">MSVASEERPAPLRELALIAALWVAYSLGRLVADGRVDEALVNADRVWEIERLLRFPGETGLQHLILRAGDLVQVANSYYAYVHFPATIGFLLWVYLRRPGHYRAVRTTLALLTGAALVVHLMFPLAPPRMLAETGLVDTGHLFGPSVYGTPGTDDLTNQYAAMPSLHVGWALLVAITLIRLTSGRRRWLWLAHPLLTLLVVVGTANHYWLDAIVVSVLLAAILRLTTTAERRPAPPPVPHPRRIRGGGPGRRPRRGSRPRTTRVPGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6I3</accession>
    <name>U5W6I3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_32825</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561160.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32825"/>
    <dbReference type="EnsemblBacteria" id="AGZ44823">
        <property type="protein sequence ID" value="AGZ44823"/>
        <property type="gene ID" value="AFR_32825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6643"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="144"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="207" mass="22195" checksum="C867CA73458ACCE1" modified="2014-01-22" version="1">MIRILLVDDQPLIRAGLRMLCDAESDLTVVGEADNGRDAVTLAARLTPDVTVMDLRMPGVDGITATGRILAARPAARVLVLTTFGDDDHLYPALTAGACGFLLKDAPPTELLDGIRRAASGDSPFSQEVLQRLVRRAVAARVEPPRPVSGLTARERDVLNLVAEGLSNTEIADRLHIGVTTVKTHITSLMTKTDSPNRVRLALFARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6K1</accession>
    <name>U5W6K1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative RuBisCO transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364458.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26690"/>
    <dbReference type="EnsemblBacteria" id="AGZ43600">
        <property type="protein sequence ID" value="AGZ43600"/>
        <property type="gene ID" value="AFR_26690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5409"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_11"/>
    <dbReference type="OrthoDB" id="1439189at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="34552" checksum="9CEC53099445ECA3" modified="2014-01-22" version="1">MNLELRHLKVVCAIAETGSVTKAASLLGLAQPALTAQLQRIERTLGGPLFERDRRGARPTALGELVLSRARVLLPAMKGLQDEAARLAGSGADESVMTRYRIGAIGGPVIGGMVHRLSEAQPDAQISTHASYYVEELATMVLAGKLDYAQVGVCGDAIPSADYGLVWQTIAVDGVCVLMPDDHPSAKGVEVDLAELADEQWVAGTGDGCFSDCFAAACARAGFTPKRMLETDIRGCVDMVESGLALAVCQPTFRPPAGLTHRPLQGAPLRWRQVLGWHPDSPAARVAPRLVGMAGEAYAEVAARNPLYIEWLEDHPQLGGVQLAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6S1</accession>
    <name>U5W6S1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative pyridoxamine 5'-phosphate oxidase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560013.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27070"/>
    <dbReference type="EnsemblBacteria" id="AGZ43675">
        <property type="protein sequence ID" value="AGZ43675"/>
        <property type="gene ID" value="AFR_27070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5486"/>
    <dbReference type="eggNOG" id="COG3871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123922_0_0_11"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019920">
        <property type="entry name" value="F420-binding_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03618">
        <property type="entry name" value="Rv1155_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="14"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="17043" checksum="075FC73AB62D7116" modified="2014-01-22" version="1">MARTVATNTPVGREELLEFLRPRHQAILMTTRKDGRPQASPNTCGVDTEGRIVISTYPERAKVANIRRDPRVSVCVLSDTFGGAWVQVDGVAEVIDPPDSVEPLVEYFRVISGEHPDWDEYRQAMRDQGKSLIRVTIERWGPVATGGFPARLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6S6</accession>
    <name>U5W6S6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ricin b lectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_33250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33250"/>
    <dbReference type="EnsemblBacteria" id="AGZ44908">
        <property type="protein sequence ID" value="AGZ44908"/>
        <property type="gene ID" value="AFR_33250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6729"/>
    <dbReference type="eggNOG" id="COG1318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030648_2_1_11"/>
    <dbReference type="OrthoDB" id="1110723at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004236">
        <property type="entry name" value="Pept_S1_alpha_lytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001316">
        <property type="entry name" value="Pept_S1A_streptogrisin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035070">
        <property type="entry name" value="Streptogrisin_prodomain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02983">
        <property type="entry name" value="Pro_Al_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00861">
        <property type="entry name" value="ALYTICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666391" evidence="5">
        <location>
            <begin position="28"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="6">
        <location>
            <begin position="398"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="515" mass="53198" checksum="D292C255B57A89DC" modified="2014-01-22" version="1">MSRPVKVLGVIAVAVTAVGLSPFTSFAAETPEPAAPTAASTFDMAPGMAAAMRRDLKLTDDQIASRLATEAAAPVVEKRLRAQLGKTFAGAWIPSGSERLTVAVTDPKAAATVRAEGAEVKIVDRSAGDLEAARQRLDKNAAEAKGTAIRGWYVDVATNRVVVMAKPGTAAAARKFAKDSGAGTVTVVDAAEAPRPLYDIRGGDQYVINGNTLCSVGFSVAGGFVTAGHCGGVNSPTLGYNNVSQGTFAGSSFPGNDYAWVRTNGNWTPQPWVNNYAGGNVTVAGSQDAAIGSSVCRSGRTSGWRCGTILGRNETIVYSQGAVSGLSRSNACAEPGDSGGSWISGNQAQGVTSGGSGNCSSGGTMWFQPVNEILGVYGLSLTTTGGGSSGQSIVSNWNNKCLDVPNSNFSDGVLVQMWNCNNSGAQKWAAVNGTLQTSNNKCLDIPWGSTASGVELQIVGCNGNPAQQFVLSAAGDLVNPQANKCLDIKDWNGNDGARIQLWDCGGTLNQKWRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6U2</accession>
    <name>U5W6U2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560038.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27195"/>
    <dbReference type="EnsemblBacteria" id="AGZ43700">
        <property type="protein sequence ID" value="AGZ43700"/>
        <property type="gene ID" value="AFR_27195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5511"/>
    <dbReference type="eggNOG" id="COG1975">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041115_0_0_11"/>
    <dbReference type="OrthoDB" id="1879870at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003777">
        <property type="entry name" value="XdhC_CoxI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027051">
        <property type="entry name" value="XdhC_Rossmann_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13478">
        <property type="entry name" value="XdhC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02625">
        <property type="entry name" value="XdhC_CoxI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC_CoxI" evidence="1">
        <location>
            <begin position="13"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="XdhC_C" evidence="2">
        <location>
            <begin position="119"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="283" mass="28820" checksum="79DE5CA672534A76" modified="2014-01-22" version="1">MTDIAERARTLTQQRRPFVHATVVRAQEPTSARAGDAAVILDDGSIEGFVGGQCAESSVRSAALGTLRDGQTLLLRVLPDGTADFPETPGALVVVNPCHSGGAIEIFLRPVLPRPVLRLAGDNPIGSAVATLAAFLDFEVTRDGTPEGATAAVIAGLGKGEEDTIRAALDAGVGFIALVASRKRGAAVLDAMGLTAGELARIHTPAGLDIGARTPPEIALSIMGEVVRAVRVDGLAASSTAVAALTATDPVCGMTVVLGPDALTANGHYFCGPGCRVAFLARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6X9</accession>
    <name>U5W6X9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative LuxR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33500"/>
    <dbReference type="EnsemblBacteria" id="AGZ44958">
        <property type="protein sequence ID" value="AGZ44958"/>
        <property type="gene ID" value="AFR_33500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6780"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="836"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="906" mass="96810" checksum="6A4157BC6DD59A30" modified="2014-01-22" version="1">MVKIFGREPEQARLTALLQHTGTPSNVLVLRGEAGVGKSTLLDWAAAAGDAGLSRSTGVQAEFTIAYAGLHRLVAQVPVLESTAAAHRTVLDAVGADGPEVRPAKVALAVLELLTAAAAERPLLLTVDDAQWLDGPSWSALAFVARRLQDDPVVLLMAMRDGEEATSRLDGSGLAELRIEPLPEAEAAGLLDARSPGLRADLGSRVLGEAGGNPLGLVELAAVAGRYGPNGLLPAWLPLPHRLERAFAQAVAELPGTVRTLLLVAAVNDGAGVDEVVAAATRLQGFPATADDLFPATAAGLIEVDGTLLIRFRHPLVRSALRQGTGVARLRAVHAALAEVLTGDQERRIWHRAEAASGPDEQLAAELTTAANAARRRGAIALAVTALERAAQLSENRHERSSRVLWAAVAAHEAGDGATVVRLLHSLENQPLSGIEQARLAWLREIFLTVGWTGASRMPAFVEIIERMRKDGETELALDSLVNVSLRCWWSNPDRATRDLIVAAAERLPVDPLDPRLVSVLALVAPLERGRLVLDQIDRLAGELTTLAEDLELLAVGASAVGALGQATVLSAAAVSALRSQGRLGTLAEALVEQATVAAQLGNVRLAITAASEARSLALETGQPRWAVVADLARGQAEALRGNGPIARELADTGERALLPIGAHPMLALVQLVRGVDALADGRYDEAYTHLHRIFDPAEVPYHPYVQFWAIGHLAEAAAGCGRLDDLSDLADELAPWAYFPVLGVALRYCTALLAPDDQAEACFQQALHADLSAWQFERARIEYAYGVWLRRQRRNVDSRHHLRAATATFAALGARPWSERARAELRAAGERVHRGPDALDELTPQELQIAELAATGLSNRDIGERLFLSPRTISTHLYRIFPKLGIRSRAELSRSLQQSFTSGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Z3</accession>
    <name>U5W6Z3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33280"/>
    <dbReference type="EnsemblBacteria" id="AGZ44914">
        <property type="protein sequence ID" value="AGZ44914"/>
        <property type="gene ID" value="AFR_33280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6735"/>
    <dbReference type="eggNOG" id="COG3001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019843_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="30196" checksum="B50552FDF9BC1AE5" modified="2014-01-22" version="1">MSGGGYAAVWRVRLTDGRDVVVKVGPPASARLLRYERGLVEAEGDYFRLVRSGAPGVPVPEVVHVGADWIATTLLPGTPLTEITDTTGVREQLGTAVAQLHRITGPHFGYTGDRPRADDWPTAYGRILEALLADAADWDVPLPPGIGELATRHAANLATVTRPALLHFDLWDGNVLAVDGRLTGLVDGERYLWGDPLLDLVSPALYHRIEDEPEHPFLRGYTAATGLVLDDAARTRLALYRVHLHTLMLAEGPSRGIPAGGERHVRLTGLLEDELRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W720</accession>
    <name>U5W720_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative membrane protein in llm 5'region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27350"/>
    <dbReference type="EnsemblBacteria" id="AGZ43731">
        <property type="protein sequence ID" value="AGZ43731"/>
        <property type="gene ID" value="AFR_27350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5544"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037599_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666395" evidence="1">
        <location>
            <begin position="25"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="398"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="530" mass="56277" checksum="B058C6619D1F16F4" modified="2014-01-22" version="1">MRQVSCTVIAGILRRVASLPICHAVTVGNDVRTAGVVAPQSPPAVVWDDQIQLHIDLIDVMTRDIPSVAHVLSIIALRLAGPCGLRAASVFTLDPDDGSLTPDATHGDVAGDDITVAGRVFRAPAGAPPVVAGHRTAVRLRIGGRTLGVLVLTGSALAALRPYVAGTIALQLASTLQVLEADRHHQHVAHAATTIRRLFEEGTSAMSVEDAGRLLATATGEAFRTERAAVHLIGPDGRIRYATGVGMTERMSEELTRNLVGKVAADSPVWQAMLELEGPLLVGDVERTRIRAGGFVETLDLRSFIAMPLLSASGPAGMVMCGNSGTRHWSGRDQALARQVAMEGALIVDSARLRQSERRHVTELTRQAYHDGLTGLPNRSQLLDRASQEVEIAMATGARLALLLLDLDGFKKINDTVGHHAGDLLLQAVGHRLQSVVRDHDVVARLGGDEFAVLLARNPDVRTASAIAARLHARLCDPYDIDGREIRIGASIGIALFPSDADDMTTLMRGADAAMYRAKREGGGVRIAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W790</accession>
    <name>U5W790_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dienelactone hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561346.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33755"/>
    <dbReference type="EnsemblBacteria" id="AGZ45009">
        <property type="protein sequence ID" value="AGZ45009"/>
        <property type="gene ID" value="AFR_33755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6833"/>
    <dbReference type="eggNOG" id="COG0412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112481_1_0_11"/>
    <dbReference type="OrthoDB" id="1889569at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DLH" evidence="1">
        <location>
            <begin position="4"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20090" checksum="1C45DAA1E7610397" modified="2014-01-22" version="1">MAEVLLFHHALGLTEGVLAFADGIRQAGHTVHTPDLYDGHTFTTVDEGLAYARKVGVQERGVRAAADLPTGLVYGGFSLGVMAAQQLAQTRPGARGALFFHSCLPVEEFGDAWPADVPVQIHAKEADPFFVDDGDLEAAQALVASAPRAELFVYPGNEHLFLDSGLADFDAEATELVSRRVKDFLAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7C7</accession>
    <name>U5W7C7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transglutaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_28195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560237.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28195"/>
    <dbReference type="EnsemblBacteria" id="AGZ43900">
        <property type="protein sequence ID" value="AGZ43900"/>
        <property type="gene ID" value="AFR_28195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5715"/>
    <dbReference type="eggNOG" id="COG1305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012121_1_0_11"/>
    <dbReference type="OrthoDB" id="1230407at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021878">
        <property type="entry name" value="TgpA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11992">
        <property type="entry name" value="TgpA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="TGc" evidence="3">
        <location>
            <begin position="430"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="689" mass="72302" checksum="B886799923DAFB26" modified="2014-01-22" version="1">MRLRTTGVVLAAACAGMLFAPVFGVTALLLPVGVPATAVLVVALLCTTPRLQEWRPVLAVLAGLLAVVETLLWPTTVAGLPTTTTLRALVTGGTDSWQLVLQSTWPARSDPALLLFVPLLVVLVAVLGVELLHRLRSPLPALLPSLALAGLTQLYASLAPGPALIAALVYAAIAGTLLFRGSWPRIVPAAAVASVAAVGAALLVPAGEPRFSLARNQAAPLPDTGLASPLDELAGRLGDPFRPVFRVRGATDVDRWPVVVLDGFDGVNWVPAGRFRRLGAELAPGPAVTVPAGERTATIELPAGQGPWLPSQTWPSRAEGVTPLVAEHQGTLLLPESTGAVTYDLSWWQPQTAVDTLAEAAIDPSAPGGLGEIGTMPPEVEALATTAVRGNRPSFATAAALENWFRDNYKIAIGSPLPAGHGWPQLTDFLLTTRRGTSEQFAAAYVALARFLGIPARLAVGYRMPVQADPDGFVTVRNGDVLAWPEVAVRGVGWVPLDPTGGAAASGATSGNGLAALAERARQSLPPPDQLPAPPVASPPAPATSSDSGRPWLLVLLALPLVAWPLGVPLTWGVRSWRRRRRPGAGAVVGAWEEIRDRLRAYGVTVSAGMTVRDLAAAAGPVTDIGTVTEIRRLAPIVDEVLWSGAAPPEERIGEAWSLVRTVRRGLARSGGRRRWWAWIDHRGLRPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7D7</accession>
    <name>U5W7D7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561376.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33905"/>
    <dbReference type="EnsemblBacteria" id="AGZ45039">
        <property type="protein sequence ID" value="AGZ45039"/>
        <property type="gene ID" value="AFR_33905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6863"/>
    <dbReference type="eggNOG" id="ENOG5031VS9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1544369_0_0_11"/>
    <dbReference type="OrthoDB" id="2053024at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024520">
        <property type="entry name" value="DUF3558"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12079">
        <property type="entry name" value="DUF3558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665621" evidence="2">
        <location>
            <begin position="34"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="177" mass="18295" checksum="7BB25F18B8547A0F" modified="2014-01-22" version="1">MNRRPLAALVAVAASLVVLSGCGALSDAAGSAAAPAPTPTSSPASDSGLGDSVKDSGDIPDPCTLLSKSEVKDLTGREISQIDEDGVKDGDSTRYCQWQQPSGQLAVFLSRTTEADFQTSIAEAEPVDGVGENAFALGGHLFVLYGTVSIDVYNRGDSDEENLAKAKKIAKVLIPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7E9</accession>
    <name>U5W7E9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560262.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28320"/>
    <dbReference type="EnsemblBacteria" id="AGZ43925">
        <property type="protein sequence ID" value="AGZ43925"/>
        <property type="gene ID" value="AFR_28320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5741"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307931_0_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="22603" checksum="67B8F89F35DA4C7C" modified="2014-01-22" version="1">MPRIRGASISQHHELVWSELAGAMRQLLLERDYDSITMGHLAARTGLARNTLYNYAGDKSALVVALTERLGRPAVERVAVVAARTADPAGERLREIIEVVLEAFTDPVMQLMFRPGAALPPVGEPKGPGSPFHEVVVETERVVRDGITGGEFRQVGDVQLVVELLSGVMRSGAERIGRDPAALAVTVDAAREIIIASLERGADQDRPAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7J4</accession>
    <name>U5W7J4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28545"/>
    <dbReference type="EnsemblBacteria" id="AGZ43970">
        <property type="protein sequence ID" value="AGZ43970"/>
        <property type="gene ID" value="AFR_28545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5787"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103066_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="39"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16866" checksum="E03AE1DAC0808141" modified="2014-01-22" version="1">MAKVNIVRPGEGEILGSGAQQIRILENGEHTDHRLGFAEVTIPPGTPSPLQHRHAQHDEGFYVLAGTFRFTVGEDQYDAGPGTWVIVPTGAPHTFANVGDENAVMLNTFTPDLYVQYFRDFKAMIDSGQPVNAETMKPLWKNYATEISSEYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W805</accession>
    <name>U5W805_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GRAM_POS_ANCHORING domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_35115</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35115"/>
    <dbReference type="EnsemblBacteria" id="AGZ45279">
        <property type="protein sequence ID" value="AGZ45279"/>
        <property type="gene ID" value="AFR_35115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35115"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7105"/>
    <dbReference type="HOGENOM" id="CLU_1607348_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019931">
        <property type="entry name" value="LPXTG_anchor"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00746">
        <property type="entry name" value="Gram_pos_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50847">
        <property type="entry name" value="GRAM_POS_ANCHORING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0572">Peptidoglycan-anchor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665792" description="GRAM_POS_ANCHORING domain-containing protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="GRAM_POS_ANCHORING" evidence="7">
        <location>
            <begin position="131"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="15749" checksum="A88376F33DA49800" modified="2014-01-22" version="1">MAAGIPAAGVTVLAALALSGSPAAATGDESAARAKQPAVMATPCSEVPRGEKCDYAYGDDDEAPGDTATDDVTDTPTRGGPGYGGESPTPSPSVTPTTPTASVDTVPPGGESPAVTPSASTPGGVSAGGTLPLTGAPMGITMGIGGLMVAAGAAAILITRRRRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8A6</accession>
    <name>U5W8A6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560493.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29475"/>
    <dbReference type="EnsemblBacteria" id="AGZ44156">
        <property type="protein sequence ID" value="AGZ44156"/>
        <property type="gene ID" value="AFR_29475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5974"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="25358" checksum="ED34B55969E12368" modified="2014-01-22" version="1">MGQLDGKIALVTGGTSGIGLATAHRFAAEGAHVFITGRDQQRLDNAVKEVGNATGVRSDVSDLADLDRLALAIREHGTGLDAVFVNAGIGEFATLDSVGWQDYQKTFNTNVGGVIFTVQKVLPLLNPGAAIVLTGSSSAGNGQPSFGLYAASKAAIRSLGRTWAGELVGRGIRVNTIVPGPTETPGLGGLVPDNPQALFQQLATGIPMGRIGRPEEIANAALFLVSDQSSFMTGSEIFVDGGAEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8P8</accession>
    <name>U5W8P8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chemotaxis response regulator protein-glutamate methylesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_30525</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30525"/>
    <dbReference type="EnsemblBacteria" id="AGZ44365">
        <property type="protein sequence ID" value="AGZ44365"/>
        <property type="gene ID" value="AFR_30525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6180"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_7_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="19"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="168"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="341" mass="37730" checksum="DD188F577773517E" modified="2014-01-22" version="1">MIRHKVDATAAEPEEKPDLILVVDDDEDIASFVAFNLKVHGFEVMRARDGQHALEVMETRRPDLAVVDWMMPNMNGVELTRRLRAEPLTSALPVIMLTAKGMTVDKVVGLTAGVDDYLVKPFDTAELIARVSSTLRRNKEFREVSPLTGLPGNARVRREIVDRMKTGKDFAVGYIDIDRFKSVNDVYGFDRGDEFITALAGSLHRAVAATGRPPVFLGHIGGDDFVFICQPEQVLPLTKRTVTDFETVADQLYDEEDARRGYIEVPDRRGNKQRAALVTLSIGVAQATVEGRRFDDAREVVAVASEMKKVAKSQPGSFVAIDRRRADSEDPEDPSSAGMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W975</accession>
    <name>U5W975_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561888.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36480"/>
    <dbReference type="EnsemblBacteria" id="AGZ45552">
        <property type="protein sequence ID" value="AGZ45552"/>
        <property type="gene ID" value="AFR_36480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7381"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_9_4_11"/>
    <dbReference type="OrthoDB" id="1669868at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="22709" checksum="226DA5DB8B0E3626" modified="2014-01-22" version="1">MHRQPAQALLIDLDGVLRRWDPAVPAAVEQKYGLEPGALLATAMNWELLRPAVAGEITDAEWMWLVASRLPLEEDVAKAAVAEWQQYRGEVDPEVLAFVREVRAAGRPVGLATNATELLRGDLDLLSLADEFDTIVSSWELKIHKPAPEFFERACAALGVEAPWVLFVDDDDRAVRGARAMKMPALRWSGAQDLPYLRKALDISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9G3</accession>
    <name>U5W9G3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Phosphate acetyltransferase</fullName>
            <ecNumber evidence="5 11">2.3.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Phosphotransacetylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">AFR_37995</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in acetate metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>acetyl-CoA + phosphate = acetyl phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19521"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The N-terminal region seems to be important for proper quaternary structure. The C-terminal region contains the substrate-binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">In the N-terminal section; belongs to the CobB/CobQ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.8" evidence="5 11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562188.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37995"/>
    <dbReference type="EnsemblBacteria" id="AGZ45853">
        <property type="protein sequence ID" value="AGZ45853"/>
        <property type="gene ID" value="AFR_37995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7684"/>
    <dbReference type="eggNOG" id="COG0280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0857">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019723_3_0_11"/>
    <dbReference type="OrthoDB" id="1301815at2"/>
    <dbReference type="UniPathway" id="UPA00340">
        <property type="reaction ID" value="UER00459"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008959">
        <property type="term" value="F:phosphate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010766">
        <property type="entry name" value="DRTGG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016475">
        <property type="entry name" value="P-Actrans_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004614">
        <property type="entry name" value="P_AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042113">
        <property type="entry name" value="P_AcTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042112">
        <property type="entry name" value="P_AcTrfase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028979">
        <property type="entry name" value="Ser_kin/Pase_Hpr-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07085">
        <property type="entry name" value="DRTGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006107">
        <property type="entry name" value="PhpActrans_proteobac"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75138">
        <property type="entry name" value="SSF75138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00651">
        <property type="entry name" value="pta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DRTGG" evidence="13">
        <location>
            <begin position="209"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="PTA_PTB" evidence="12">
        <location>
            <begin position="367"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR006107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ45853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="688" mass="72058" checksum="64E32696F6F194A1" modified="2014-01-22" version="1">MASSVYVAGLGPGVGKGTVALGLVELLSRQVARIGVFRPLVSGTGEDPLLTLLTTRYPVVAPYAESFGVTVAEASALVADGKREELISRIVERYREVERQCGSVVVIGSDFSDGQDDGHDELPRELAFNARLATEFGSVVVPVVSGQGKRTDLNAAVRSAYHSLVDLNATVLAVIANRVSEGVTPPDGLPVPVWAIPEVPAIAAPTVAEVAAALDATVVTGGEAALDRDVLDYVVGAAQVPAVLDHLTDGALLITPGDRADLLVAASAAHAAGNVTLAGLVLTIGEFPDPRAVRVIERLNTGLAMLVVQSDSYHTISAAGRIAAKLSAGTPRKVEAALGAFEKHVDTAELTRLLDVTRSSRVTPLMFENDLIDRARADRRHLVLPEGTDERILRATETLLRRGVADLTLLGDPAEITRRAREFGVEIGAARLVDPATSGWRDGFAEKYAELRKHKAVTLDLAYDVVRDVNYFGTMMVAAGLADGMVSGANHTTAATIRPAFEIIKTVPEVSVASSVFFMLLADRVLVYGDCAVNPDPDAAQLADIALSSAQTAAAFGIEPRVAMLSYSTGSSGSGADVEKVAAATALVRERRPDLPVEGPIQYDAAIDPGVAATKLPESVVAGKATVFVFPDLSSGNNTYKAVQRSANAVAVGPVMQGLRRPVNDLSRGATVKDIVNTVAITAIQARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9G9</accession>
    <name>U5W9G9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative enoyl-CoA hydratase/isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562003.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37065"/>
    <dbReference type="EnsemblBacteria" id="AGZ45667">
        <property type="protein sequence ID" value="AGZ45667"/>
        <property type="gene ID" value="AFR_37065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7499"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_11"/>
    <dbReference type="OrthoDB" id="1498685at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="257" mass="26886" checksum="A474517BEAD3CB84" modified="2014-01-22" version="1">MGEFVRLETSGGIGTIRLERPPMNALNTQVQEELRASAQAATADPEVRAVIVWGGEKVFAAGADITDFTTTTYQEMTVRAAALSSAFDAVAKIPKPVVAAITGYALGGGCELALACDWRVVADDAKLGQPEIKLGIIPGAGGTQRLARLIGPAKAKDLIFSGRMVDAEEALRIGLADRVAPASEVYATAAALLKPYLNGPALALRAAKLAVDGGLNTDLASGLAWESQLFAGLFATDDRIEGTTAFVEKRKPKFTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA42</accession>
    <name>U5WA42_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_38800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562348.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38800"/>
    <dbReference type="EnsemblBacteria" id="AGZ46014">
        <property type="protein sequence ID" value="AGZ46014"/>
        <property type="gene ID" value="AFR_38800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7851"/>
    <dbReference type="eggNOG" id="COG0312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_2_11"/>
    <dbReference type="OrthoDB" id="806701at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="2">
        <location>
            <begin position="25"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="4">
        <location>
            <begin position="118"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="3">
        <location>
            <begin position="235"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="480" mass="50952" checksum="3E9FE4C3A9D8606B" modified="2014-01-22" version="1">MSVDHFDEAGTAVQAALDAGARYADARVMLRRTETMSARDGEVEDLSSDESAGIGVRALVGSSWGFYAVPDLSDSAARAAGRRAAEIAAASAIVPGPAIDLVPAGAGTASWASECVIDPLSVPLSTKGDLLVAATAAAKDAGADLAEGIYQIWDTRKWFVSSDGHRIDQHIRECGAGITASAYGDGEIQRRSWPSHRGQYGTRGWELVDELALTDNAARMADEARALLTAPLCPSGETTLILGGEQLALQIHESVGHAIELDRILGWEAAFAGTSWLDLDQLGTLRYGSDLMNITIDPTIPGALGSFGYDDEGSPAVKRDAVRNGRWVGVLAGRDSAAMAGLDYAGSVRSDGWARLPMVRMTNVGLEPGPHTLDEIIAATDDGVLMDINRSWSIDDRRLNFQFGCEIGYEVKNGKRGRMLRNPTYTGIGPKFWQSMDMLSSETVAWGTPNCGKGQPGQIGHTGHPAAPARFTNVRVGVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA62</accession>
    <name>U5WA62_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_38900</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562368.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38900"/>
    <dbReference type="EnsemblBacteria" id="AGZ46034">
        <property type="protein sequence ID" value="AGZ46034"/>
        <property type="gene ID" value="AFR_38900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7872"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_1_2_11"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="7"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="130"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="244"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="44254" checksum="6F899ECF4611BF76" modified="2014-01-22" version="1">MDFAFDEKTEDYRKRLLGFLDEYVYPAEATFDEDYAGDWAPPPVITRLQAQAKLAGLWNLFLPGEHGAGLTNLQYAPLAELTGRSPAIAPPALNCAAPDTGNMEVLAQFGSPAQQERWLRPLLDGTIRSAFAMTEPQVASSDATNIGTRIIRDGNDYVINGRKFYISGAMNPNCQIFIVMGKTDPDAARHVQQSMILVPRDTPGLTIKRGMTVFGYDDGDHGGHAEMLFEDVRVPAENLIGEEGSGFAISQARLGPGRIHHCMRLIGMAERALELMCRRSLTRTPFGKPLAEQGVVQEWIAESRVRIEQARLLVLKAAWLMDTVGNKGAHTEIQAIKIIVPSTTEWIIDKAIQAHGAAGVGQDTPLARLWAQTRTLRLADGPDEVHKRSLARRELNRYRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAC5</accession>
    <name>U5WAC5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Asparagine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_33190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparagine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561233.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33190"/>
    <dbReference type="EnsemblBacteria" id="AGZ44896">
        <property type="protein sequence ID" value="AGZ44896"/>
        <property type="gene ID" value="AFR_33190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6717"/>
    <dbReference type="eggNOG" id="COG0367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_3_1_11"/>
    <dbReference type="OrthoDB" id="610569at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017535">
        <property type="entry name" value="Asparagine_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03104">
        <property type="entry name" value="trio_amidotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="7">
        <location>
            <begin position="2"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="366"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="590" mass="64874" checksum="81A7060C9F1EC0D1" modified="2014-01-22" version="1">MCGIGGELRYDAQAADGDAVRRMLPCLESRGPDGEGLWQRGPIAFGHRRLKIIDLSEAGGQPMTDEQLGLTLVFNGCIYNYQQLRDELRGYGYTFASTSDTEVIIKAYHRWGADCVQHFLGMFAFAIAEHATGTVMLARDRLGIKPMYLAETPGRLRFASTLPALLAAGDVDTSIDKVALQHYMTFHSVVPAPRTLVAGVRKLPPATVRIIKSDGTSTERVYWEADHTRTLIKSREEWAEAIHESLRTAVERRMVADVPVGVLLSGGLDSSYIVALLAEQGQRGLTTFSIGFESAAGESGDEFAYSDIIAKQFDTTHHQIRIGKDRFLPAVARTVAAMSEPMVSHDCIAFNLLSEDVSQHVKVVQSGQGADEILAGYSWYPPLRDVPRDQAVDAYAREFFDRPHSELARQLSLEWLLDADVSREFVAASFARAGATSSVDAALRLDSQVMLVDDPVKRVDNMTMAWGLEARVPFLDHELVELAAACPPELKLAQGGKGVLKDAARGVVPDEVIDRTKGYFPVPGIRHLEGPMLDMVREALHAPAARARGLFRTEYVDALLADPNTARTTLGANQLWQLALLEMWLQDKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAI2</accession>
    <name>U5WAI2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Duffy receptor gamma form</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46124">
        <property type="protein sequence ID" value="AGZ46124"/>
        <property type="gene ID" value="AFR_39350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7961"/>
    <dbReference type="HOGENOM" id="CLU_144124_0_0_11"/>
    <dbReference type="OrthoDB" id="1856590at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="113" mass="12230" checksum="F8E449C97D8DB582" modified="2014-01-22" version="1">MEEFAATLANEVASGYAPHLDQVTTAMLTRVPKGNPAFPELAGFLDRHHEVQQQTFSNTFNFRDGTHHFATAAQTISAEYADTDAYAHAKVNDVTQAFTDASNPLKSEGGVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAN4</accession>
    <name>U5WAN4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative trypsin-like serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_40095</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562605.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40095"/>
    <dbReference type="MEROPS" id="S01.101"/>
    <dbReference type="EnsemblBacteria" id="AGZ46273">
        <property type="protein sequence ID" value="AGZ46273"/>
        <property type="gene ID" value="AFR_40095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8114"/>
    <dbReference type="eggNOG" id="COG5640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_0_11"/>
    <dbReference type="OrthoDB" id="1468733at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665867" evidence="3">
        <location>
            <begin position="32"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="39"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="27210" checksum="F03EDFDBAE857A8E" modified="2014-01-22" version="1">MVRWRRILGGLAAALTVASMGTLVSASPASAEPDGPQKVVGGTRATQGEFPFMVRLSMGCGGALYSPTLVLTAAHCVNRTGATTSITATIGVVDLNSTSKVTRRSNYVYRAPGYNGNGKDWALIRLSSPVTGFATLPIATTTAFDSGTFTIAGWGAATEGGGQQRYLLKATVPFVSDSTCNSSSMYNGQIVATDEICAGYTAGGVDTCQGDSGGPMFRRNASNAWIQVGIVSWGDGCARPNKPGVYTQVSYFSSAIASAAASLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAT8</accession>
    <name>U5WAT8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoenolpyruvate carboxykinase [GTP]</fullName>
            <shortName evidence="8">PEP carboxykinase</shortName>
            <shortName evidence="8">PEPCK</shortName>
            <ecNumber evidence="8">4.1.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GTP-dependent phosphoenolpyruvate carboxykinase</fullName>
            <shortName evidence="8">GTP-PEPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pckG</name>
        <name evidence="11" type="ORF">AFR_39290</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10388"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.32" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39290"/>
    <dbReference type="EnsemblBacteria" id="AGZ46112">
        <property type="protein sequence ID" value="AGZ46112"/>
        <property type="gene ID" value="AFR_39290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7949"/>
    <dbReference type="eggNOG" id="COG1274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028872_1_1_11"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004613">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00819">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00452">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018091">
        <property type="entry name" value="PEP_carboxykin_GTP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008209">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035077">
        <property type="entry name" value="PEP_carboxykinase_GTP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035078">
        <property type="entry name" value="PEP_carboxykinase_GTP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561">
        <property type="entry name" value="PTHR11561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00821">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17297">
        <property type="entry name" value="PEPCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001348">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="SSF68923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00505">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PEPCK_N" evidence="10">
        <location>
            <begin position="12"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PEPCK_GTP" evidence="9">
        <location>
            <begin position="235"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="376"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="262"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="506"/>
            <end position="509"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ46112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="599" mass="66016" checksum="DA9C102982A785A5" modified="2014-01-22" version="1">MPPTAHAKLLTWVDEVAALTAPDRVVWCDGSDEEWARLTDTLVEAGSLVRLDPVKRPNSFWARTDPGDVARVEERTFICSVDPADAGPTNNWTAPDEMKATMTELYRGCMRGRTMYVVPFCMGPLDAPSPMFGVEITDSAYVVTSMRIMTRMGTPVLAAMGDDADFVPALHSVGRPLAEGEADVAWPCNETKYISHFPETREIWSFGSGYGGNSLLGKKCFALRIASVAARDEGWLAEHMLIMKLTSPEGQVKYIAGAFPSACGKTNLAMLEPTLPGWKVETVGDDIAWMRFGEDGRLWATNPEYGLFGVAPGTDQHTNPNAMRTLARGNSVFTNVARTDDNDIWWEGMGEPPAHLIDWKGNDWTPDSDEPSSHPNSRFCTPINQCPILAPEYDDPRGVPIDAILFGGRRKTTVPLVTEARDWVHGVYFGATLSSETTAAAVGQVGVVRRDPMAMLPFIGYHAGDYFNHWIEMGKAASGDAEKLPKVFYVNWFRRDEDGGFLWPGFGENSRVLKWIVERLDGRGDAIETPIGHVPTADAIDVTGLDISRADLEAVLRVDTDEWLAEIPQVEEWFAKFGDKLPAVLWSELDALKARLNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB18</accession>
    <name>U5WB18_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coagulation factor 5/8 type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561463.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34340"/>
    <dbReference type="EnsemblBacteria" id="AGZ45126">
        <property type="protein sequence ID" value="AGZ45126"/>
        <property type="gene ID" value="AFR_34340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6948"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043495_0_0_11"/>
    <dbReference type="OrthoDB" id="811477at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006584">
        <property type="entry name" value="Cellulose-bd_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03422">
        <property type="entry name" value="CBM_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00606">
        <property type="entry name" value="CBD_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666476" evidence="2">
        <location>
            <begin position="36"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="3">
        <location>
            <begin position="38"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="456" mass="47626" checksum="589D9D218AEA4A52" modified="2014-01-22" version="1">MHRTHRQSRKLLGISLAAVLTGALSLTIGGGIAEAATATVQAEAYAAQSGVQLEATGDTGGGQNVAYLANGDWMRFDGVDLGPAGALTVSARVASAIGTGSVELHTGSATGPLLAQFPITATGGWQNWATLTATASTHPTGAQTVFAVLKNSGTGNFVNINWFSFGTGDTPSGDGWVTLDQAKWNAQLAEFRAMAPRPVPADSVRVPEFNASCLYSHSKPDDPIVFPGMAGASHMHSFIGNKSTDANTTTDTLLRNAGTSCKPAEDLSAYWIPTLYERNQVVEPKDVVVYYGSRLVDSAATVPFPQGFRMIAGDARLQAPTPAGSVNQFYCAGAGGEIGRSADGNWPRCAPGATLMFQLVFPDCWDGVHLDSPTHKAHVAYTYDGTCGGAFPVAIPSISFLIAYPTAGSADGFRLASQMASSMHGDAFLAWDNAALGHRVKNCVVQRAKCNTAGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB19</accession>
    <name>U5WB19_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative flagellum-specific ATP synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fliI</name>
        <name evidence="5" type="ORF">AFR_40450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562676.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40450"/>
    <dbReference type="EnsemblBacteria" id="AGZ46344">
        <property type="protein sequence ID" value="AGZ46344"/>
        <property type="gene ID" value="AFR_40450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8187"/>
    <dbReference type="eggNOG" id="COG1157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_5_1_11"/>
    <dbReference type="OrthoDB" id="875807at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030257">
        <property type="term" value="C:type III protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030254">
        <property type="term" value="P:protein secretion by the type III secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005714">
        <property type="entry name" value="ATPase_T3SS_FliI/YscN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040627">
        <property type="entry name" value="T3SS_ATPase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18269">
        <property type="entry name" value="T3SS_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01026">
        <property type="entry name" value="fliI_yscN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="155"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="435" mass="45786" checksum="E15908ADEABAECE2" modified="2014-01-22" version="1">MTDVFRNRINAALSAAQPLSRGKVTGAVGLRVTVSGLEARVGELLQIGEGDEAVLAEVAAIDGERLSCLPLGPIAGMSTGSPVVSTGGPLRVAVGPDLRGRILDGLGRPMDGGPPLKGEMVSIEAAPPSAMERQLVDAPMPLGVRVLDTLVPCGRGQRIGIFAGSGVGKSTLMSMITRGTTAELNVVALVGERGREVREFIEHDLGPEGLARSVVVIATSDTPPLVRLRAGSVATRIAEYFRDQGGDVLMMMDSVTRAAMAQREVGLSVGEPPATRGYPPSVFAMLASLLERAGPGARGSITGLYTVLVEGDDHNEPIADAARSILDGHIVLDRKLATAGHFPSIQALDSISRVANRITTPEQRNDATELRRMMAAHRDVRELVEIGAYVPGTNPEADRATAIWPQITSFLRQGLDEKVSAAEAWQALRQLIAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBA0</accession>
    <name>U5WBA0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40850"/>
    <dbReference type="EnsemblBacteria" id="AGZ46424">
        <property type="protein sequence ID" value="AGZ46424"/>
        <property type="gene ID" value="AFR_40850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8270"/>
    <dbReference type="eggNOG" id="COG4339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051795_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009218">
        <property type="entry name" value="HD_phosphohydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21174">
        <property type="entry name" value="PTHR21174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035170">
        <property type="entry name" value="HD_phosphohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="22759" checksum="E8BB3904168BC27D" modified="2014-01-22" version="1">MTSLGDRWVAAARGAGATAGDADLAAAGEYLLARWSEPQRQYHTVAHLTAVLDVIDEFATLAPGADRVRLAAWLHDAVYDPRALGDTNERDSAEFAEGLLTTLGAPDEVAAEVARLVGLTAGHATNDDDPDGELLCDADLAILASTPDDYAAYTAAIRREYAHVPDEAFRGGRTQVLKALLELPSIYRHAPLRQAWEDRARANLEAELKTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBH1</accession>
    <name>U5WBH1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_35200</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561632.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35200"/>
    <dbReference type="EnsemblBacteria" id="AGZ45296">
        <property type="protein sequence ID" value="AGZ45296"/>
        <property type="gene ID" value="AFR_35200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7122"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_2_2_11"/>
    <dbReference type="OrthoDB" id="550338at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="8"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="268"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="398" mass="42313" checksum="48F96A73C2D0EA78" modified="2014-01-22" version="1">MPRVSREVVFVDGVRTPFGKAGGMYAETRADDLVIRCIRELMRRNPQLPPERVDEVAIAATTQTGDQGLTIGRTAALLSGLPKTVPGYAIDRMCAGAMTAVTNVAGGIAMGAYDVAIAGGVEHMGHHPMGEGVDPNPRILAEKLVDPSALVMGSTAENLHDRLPQITKERVDAYGLASQEKTAKAYANGKLQPDMVSVAIRNAEQGWGLATVDEAPRETSMEKLATLKTPFRPHGKVTAGNAAGLNDGATAALLADEETARELGLPVAMRMVSYGFVGVEPEIMGYGPIPSTEKALRLAGLTIDDIGLFELNEAFAVQVLAFLDHYGIADDDPRVNPWGGAIAIGHPLASSGVRLMTQLARHFAEHPEVRYGINAMCIGIGMGGTVIWENPNWEGNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBQ3</accession>
    <name>U5WBQ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41450"/>
    <dbReference type="EnsemblBacteria" id="AGZ46544">
        <property type="protein sequence ID" value="AGZ46544"/>
        <property type="gene ID" value="AFR_41450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8387"/>
    <dbReference type="eggNOG" id="COG1172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038566_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="428" mass="44372" checksum="09EBFF608964A07A" modified="2014-01-22" version="1">MLCRGEEVREMAYDETTYRRNTDDQGDNDPAAYRTGFGAADFRARRRDLDPDDTGTRLTEPVAEPVRQDEVDDAGRDRLGIHIGWEIVLLLAVAAIAYLLYRLDPASLRRPALDSLLISGASIGLLALGAGLTLRAGVPNLAVGPIALAAALHFAENGDDGLVKAAVPALIVAAVGGLVIGLFVIVLHVPGWAATLGAAMGVIVYNQLRTVPVTVQGDYDPSNQAFYLFGGFALLAVIGGALGTVGPIRRMVGGLRQAGDPARRRGAGSAFPVLVSLILSSVFAVGAGILIAAGSTAPITPGTGLEWTGIAFGAALLAGTSAYGRRGGIFGTLLAVAGIALFLDYADRRNFDIALFAIAACAIGGGLIVTRLVETYGRPLPVPMVGEDWNAAASSGTTWTPDLPTAAAPPQQQGAPLRWDEGPWGSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCA9</accession>
    <name>U5WCA9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 12">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="12">ASA dehydrogenase</shortName>
            <shortName evidence="12">ASADH</shortName>
            <ecNumber evidence="7 12">1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">asd</name>
        <name evidence="14" type="ORF">AFR_41740</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-L-aspartate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="7 12"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562933.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41740"/>
    <dbReference type="EnsemblBacteria" id="AGZ46602">
        <property type="protein sequence ID" value="AGZ46602"/>
        <property type="gene ID" value="AFR_41740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8447"/>
    <dbReference type="eggNOG" id="COG0136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_0_0_11"/>
    <dbReference type="OrthoDB" id="1799040at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005986">
        <property type="entry name" value="Asp_semialdehyde_DH_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01296">
        <property type="entry name" value="asd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="13">
        <location>
            <begin position="7"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="12">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="14"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="42"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="162"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ46602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="356" mass="37987" checksum="F3F9D843907E2F14" modified="2014-01-22" version="1">MAQSQPTLAVVGATGSVGTVMRELLTSRRNVWGEIRLIASARSAGKELSCRGEQIIVRELTPDVFDGVDVAMFDVPDDVSTAWAPVAVERGVTVVDNSSAFRMEPDVPLVVPEVNKEELSRRPRGIVANANCTTMAMIMAIAPLHHEYGLRELVLASYQAVSGAGQIGVDTLHDQLTKVAGDRSLGSRPGNVRQAVGDDLGPFTAPLALNVVPWSGDLAADGWSSEELKLRNESRKILRLPDLKVSATCVRVPVVTGHSIAVHAVFGSEVDAEGARQVLRNAPGVILVDDPEAGEYPMPIDAVGTDPSWVGRIRRAMDDPRALDLFITGDNMRKGAALNTVQIAELLAAEFAKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCC4</accession>
    <name>U5WCC4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="6">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">coaD</name>
        <name evidence="8" type="ORF">AFR_36550</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the bacterial CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561902.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36550"/>
    <dbReference type="EnsemblBacteria" id="AGZ45566">
        <property type="protein sequence ID" value="AGZ45566"/>
        <property type="gene ID" value="AFR_36550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7394"/>
    <dbReference type="eggNOG" id="COG0669">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100149_0_1_11"/>
    <dbReference type="OrthoDB" id="1846503at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02163">
        <property type="entry name" value="PPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00151">
        <property type="entry name" value="PPAT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001980">
        <property type="entry name" value="PPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF1">
        <property type="entry name" value="PTHR21342:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01020">
        <property type="entry name" value="LPSBIOSNTHSS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01510">
        <property type="entry name" value="coaD_prev_kdtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="122"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="159" mass="17543" checksum="670CA597E71FAC96" modified="2014-01-22" version="1">MRRAVCPGSFDPVTNGHLDIVGRASRLFDEVIVGVLINHSKTGLFTVEERIEMLRQATASYGNVRVESFHGLLVDFCRAQGAAVVIKGLRAVSDFDYELQMAQMNIGLSGVETLFMPTNPLYSFLSSSLVKDVVKWGGDASSYVPDFVGERLVTRLRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCL9</accession>
    <name>U5WCL9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46717">
        <property type="protein sequence ID" value="AGZ46717"/>
        <property type="gene ID" value="AFR_42315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8564"/>
    <dbReference type="HOGENOM" id="CLU_1259217_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666279" evidence="2">
        <location>
            <begin position="27"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="22282" checksum="002BD082AEDABC64" modified="2014-01-22" version="1">MRRSLVLPLTALVGVAAIVPISVAAASSTPPKALDVVVPPPVEERFGVVRYDTAAQRWGVLNNATFQSSGLGSVSCSSSTGILTVGFTPLGTIGTFTVDEDDAYAGRYDAGAALTTRSMAITFRKATTGAVVKCNASELRIANSSLQVWVKGAAAVPPTTVPTSPTRPGPASPTAPPTETTGPPTPPPTTQPTITPPTIDPGTDPEDTPPAFPTPPPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCN8</accession>
    <name>U5WCN8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycogen synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_42415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42415"/>
    <dbReference type="EnsemblBacteria" id="AGZ46737">
        <property type="protein sequence ID" value="AGZ46737"/>
        <property type="gene ID" value="AFR_42415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8584"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_35_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="3">
        <location>
            <begin position="58"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="425" mass="46015" checksum="8EFF8BB00DA994A9" modified="2014-01-22" version="1">MADRTLPQVSGERLDLGKLGHALTVAFPGVKVVVAHNRYREAIPSGENVIVDTEIAQLTAAGIEVLPFQRASDSIGDLPLAQKALLPLSPIYGRAAQRDLSELLQRERPDLLHLHNPYPLLSPWVVRTAHEHGVPVVQTVHNYRQVCSSGLYFRDGHNCHDCRGKAFGWPAVVHKCYRGSAAQSALMATTLAVHRPTWKSVDRYIALTGRIAAHLRDYGIPDERIVIKPNGLPDPGEPAPLGDGFLYAARLSPEKGLELLLDAWRRHPDGSLGPLRIAGDGELRPLAEQAAAERDDVTYLGALDRTGMEAARDQASVVVAVPTWNDVLPTVILEAMAAGRPVLGTDVGGIPYLLGIDESAQVAGWVVKPDAETLAAALPIARVDAAAVAPNARTRYLQAFHPDVVTRRLIDVYTSLTENSHPAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDY2</accession>
    <name>U5WDY2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase s8 and s53 subtilisin kexin sedolisin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_39360</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39360"/>
    <dbReference type="MEROPS" id="S08.121"/>
    <dbReference type="EnsemblBacteria" id="AGZ46126">
        <property type="protein sequence ID" value="AGZ46126"/>
        <property type="gene ID" value="AFR_39360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39360"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_13_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666293" evidence="7">
        <location>
            <begin position="28"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="8">
        <location>
            <begin position="52"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="306"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="375" mass="36930" checksum="BCC4DBB1AE1637A4" modified="2014-01-22" version="1">MTRFIAAVVAAVLAGSSAAAYGAPAIAADAVRNKQWHLKYLKVEEAHRSSTGKGITVAVIDSGVAGHPDLAGSVLDGTDFVKRGADGRTDRSGHGTGMAGLIAAHGNGNNGALGIAPDAKVLPIRVLDRGRRNAEVGPAIDYAVKKGAKVINLSLGGSIDPATLSAVEEAKAADVVIIASAGNKPAEFDVIAPAFLKDIVAVGATNRSGEKADVSVSGAALDLMAPGEDIESTDAGGGYRVGTGTSDAAAIVSGAAALIRSKYPDMSAPEVVARLESTATDKGAPGVDKDYGHGVLNLVAALSDSPGSGPAPSAAPSASASSTPTVAPTVAAPPDNELAGSTTPLIVGGIAAVVVLLGGLVGAFLLVRRRSGSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE09</accession>
    <name>U5WE09_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562490.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39510"/>
    <dbReference type="EnsemblBacteria" id="AGZ46156">
        <property type="protein sequence ID" value="AGZ46156"/>
        <property type="gene ID" value="AFR_39510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7993"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_926794_0_0_11"/>
    <dbReference type="OrthoDB" id="1452155at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="2">
        <location>
            <begin position="48"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="29671" checksum="324985B15085038D" modified="2014-01-22" version="1">MPKSDKPAKVAAVTAPARLGVRKTYKLYIGGAFPRSESGRTYLVDGDNVALASRKDARDAVVAARKAQPKWAGATAYNRGQVLYRIAEMIEARRGEFVTLGVPATEVDAAVDRWIWYAGWSDKIAQVHGGANPVAGPFFNISAPEPTGVVGVVAPESFLGLVSVIAPAIVTGNTVVVLAASPQVAVTLAEVLATSDVPGGVVNILTGHAAETAPWLASHDDVNALDLTGVADTGLAADLERAAAGNLKRVLRPPVDTPDFTADPGLHPMTALLETKTVWHPKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEF4</accession>
    <name>U5WEF4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator ycf27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562653.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40335"/>
    <dbReference type="EnsemblBacteria" id="AGZ46321">
        <property type="protein sequence ID" value="AGZ46321"/>
        <property type="gene ID" value="AFR_40335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8163"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_8_11"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="10"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="133" mass="14290" checksum="1826CE5D71C692C2" modified="2014-01-22" version="1">MTEVPQERPMVLVVDDEEDLRDIMRRMLERRGFATLVAGDADQAIAVCREHPGVIDVLVTDLGLPGVSGGDLARTASGLRPEMGVVYISGLPKDIAVTKGLIAEDALLVKKPFTSDLLLEALRSILAQRAPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF91</accession>
    <name>U5WF91_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563036.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42255"/>
    <dbReference type="EnsemblBacteria" id="AGZ46705">
        <property type="protein sequence ID" value="AGZ46705"/>
        <property type="gene ID" value="AFR_42255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8554"/>
    <dbReference type="eggNOG" id="ENOG5033XY6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097567_1_0_11"/>
    <dbReference type="OrthoDB" id="1818589at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="98"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="197" mass="22023" checksum="67B9E0D208A24B55" modified="2014-01-22" version="1">MQLTRDFPDPARKGVVLSSEYRQEKVEEILEPWTSAPWPAGLLSYSIYQDADGDLIRDYSQWTDEEALDNSGRAGDRYHLYRGSRSEDGRVPGAMVAVRVDTDSAELARTWVDAVFEALERDRNLPAGGIGAFFHISTDGRQVLNYAEWETAEDHRRALAANSGSVSAGPLWDRVRTMPGVRQVSVIRYVLHATLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFD4</accession>
    <name>U5WFD4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562952.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41835"/>
    <dbReference type="EnsemblBacteria" id="AGZ46621">
        <property type="protein sequence ID" value="AGZ46621"/>
        <property type="gene ID" value="AFR_41835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8466"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087580_2_1_11"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="21326" checksum="F9A3D1BBF0A77B1D" modified="2014-01-22" version="1">MSLTNPFGDLQVTDPQAMRALAHPVRLAILSFLQRNGPATATVLSPHVGATPSVTSWHLRHLAGFGLVTDADPDEVPGDRRQRWWKAKARGFSVSAGEDPESTAAARVLGNQLAAVAQQQVSDWVTDVAPGLPGEWARASGISNTSVPLTSEELTRLSAQIDELIAPYVHRAEADAPAGARVVRILRHYLPSASDLGDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFK9</accession>
    <name>U5WFK9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_42855</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyltransferase 87 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563155.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42855"/>
    <dbReference type="EnsemblBacteria" id="AGZ46825">
        <property type="protein sequence ID" value="AGZ46825"/>
        <property type="gene ID" value="AFR_42855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8669"/>
    <dbReference type="eggNOG" id="COG5650">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028876_1_0_11"/>
    <dbReference type="OrthoDB" id="1529783at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018584">
        <property type="entry name" value="GT87"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016570">
        <property type="entry name" value="UCP010361"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09594">
        <property type="entry name" value="GT87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010361">
        <property type="entry name" value="UCP010361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="514" mass="56515" checksum="750C536666A1DAD1" modified="2014-01-22" version="1">MSAPSSPEADRPDLPAESDGFVRGLSQAIGGPLGDHAVRPGPRPGRFWNAQRIILALVCMTLALSWVQKSPCQDGDWQKNVQYTRFCYTDVLALYYAEGLNEGKVPYKDHPVEYPVVTGYFMGALGLPVHALGEKYPDINQGKWFYNINALVLSILAVASAAMMLALRRRRPWDVAIFALSPILLVTATVNWDFLAIGLALFGLYAWARRWPILAGFLLGLGGAAKLWPLFILGPLLVLGLRSKRVPQFLLATGAAIVAWLAVNLPVAILYRDSWDRFFNLNRERPIDWGTSWYIGRYLDSKWNTGVAGDQGPFQWLSDHVPVLNNISYALTFLACAAIGVLCLLAPRRPRLSQVAFLVVAAFLIFSKVWSQQYVLWLLPLIVLARPRWGAIIAWSVAEIGYFTAFYAELLGAGGKPVIPEGTFVLASSLRLITVAILCGLVIREIWRPELDPVRQTYADDPDGGVFDGAPDAGWLSSLRQRLRLEPRPAAVESASESAPPPPPPPPPTPAPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNF4</accession>
    <name>U5VNF4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_00125</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357258.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00125"/>
    <dbReference type="EnsemblBacteria" id="AGZ38314">
        <property type="protein sequence ID" value="AGZ38314"/>
        <property type="gene ID" value="AFR_00125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.26"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_3_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="37"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="135"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="190" mass="21063" checksum="11325187B6B9FE2B" modified="2014-01-22" version="1">MRRKKTPRAEPVTELSSDHELLRAVAGEQLEALKLLHQRHAPWLKARLTRRCADPDVVDAAIQDTFLAVWRDAHRYRDVGADPAGWIWTIAMRRLVSTLRGPANRWLGGGLIPDKDATTPSAEDLVLLRVEHGDLGAALDRLSPELRAVIQATAVDGLTVKEAARLLGVPEGTAKTRLMRARARLRELLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPR1</accession>
    <name>U5VPR1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LUD_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03295"/>
    <dbReference type="EnsemblBacteria" id="AGZ38948">
        <property type="protein sequence ID" value="AGZ38948"/>
        <property type="gene ID" value="AFR_03295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.663"/>
    <dbReference type="eggNOG" id="COG1556">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090664_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LUD_dom" evidence="1">
        <location>
            <begin position="99"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="20616" checksum="21BA2441779D95F2" modified="2014-01-22" version="1">MVLGRIRAALGDDAAPPAGIPRDYRRGDQPADLDLLVHRLEDYKAVVHRGVSVAETVAALAPDPLIVPPGINPDWLPAGVEVLHDDGLSADVLAVAGGVLTAATVAVAETGTIVLDGSPDQGRRVISLLPDLHICVVRADQVVASVPEALTRLAPQRPLTWISGPSATSDIELNRVEGVHGPRRLHVILLEASDHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQS3</accession>
    <name>U5VQS3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="13">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AFR_05145</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12 13">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="13"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358840.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05145"/>
    <dbReference type="EnsemblBacteria" id="AGZ39318">
        <property type="protein sequence ID" value="AGZ39318"/>
        <property type="gene ID" value="AFR_05145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1041"/>
    <dbReference type="eggNOG" id="COG1085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029960_1_1_11"/>
    <dbReference type="OrthoDB" id="1064872at2"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019779">
        <property type="entry name" value="GalP_UDPtransf1_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="PTHR11943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00117">
        <property type="entry name" value="GAL_P_UDP_TRANSF_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 13" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="6 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GalP_UDP_transf" evidence="14">
        <location>
            <begin position="43"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="GalP_UDP_tr_C" evidence="15">
        <location>
            <begin position="215"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="10 12">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AGZ39318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="363" mass="40496" checksum="547F31D6A8D6AECB" modified="2014-01-22" version="1">MISKRTAIRLSDGRELIYFDETADSGRAKWPDTRQLPPPPPTSQLRYDPLVDEWVAVAAHRQTRTFLPPTDKCPLCPSTPDFASEIPAPDYDVVVFENQFPSFSDRVKAGEITELTDMVPVRPGVGRCEVVCFTSDHNSSFGALTPERVRTVVDALADRTTEMSALTGVAQVFPFENRGIEIGVTLSHPHGQIYAYPMVPPRTQAMLRAARKHAEHTGGRNLYADVLAAERKAQVRVVTANEHWTAYVPAASRWPFEIQLAPNRQVLDIPALTDAERDAFGPIYLDILKRFDALFDRPMPYISAWHQAVTGEGRDLGYLHLQLFSIRRAADKLKFLAGSESAMGAFINDIVPETAAQILRDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRE8</accession>
    <name>U5VRE8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glucosamine 6-phosphate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PGI/PMI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358965.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05725"/>
    <dbReference type="EnsemblBacteria" id="AGZ39434">
        <property type="protein sequence ID" value="AGZ39434"/>
        <property type="gene ID" value="AFR_05725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1161"/>
    <dbReference type="eggNOG" id="COG0794">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059687_2_0_11"/>
    <dbReference type="OrthoDB" id="1179564at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05637">
        <property type="entry name" value="SIS_PGI_PMI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019490">
        <property type="entry name" value="Glu6P/Mann6P_isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10432">
        <property type="entry name" value="bact-PGI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="58"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="407" mass="41706" checksum="DB1B2249F5839556" modified="2014-01-22" version="1">MAGPIEGAAGLTGRRVADESLLEDEKTMLENDPGGMLRATASAGAQVRESAALAAEANLTVLADEGRPRAVVVAGIGTAGLTGSILATVAGPRCPVPVIGHRSAGVPGWVGAADVVIAVSASGRSPEALAAADAAARRGARLVAIGNPDSELQAMAERARAPFIPVPRRAPARASLWGLAVPVLLAARSLGLVKVNEADLAETAARLDADAERCRANAESFTNPAKSLALGLAGSIPIVWGSSPLATVAARRFADTLAANARYPVMAGALGEAGRGRVGLLDGPFGGLAESSRDIFADPDEEEEANTRLRLVVFRDGGLNPEDEADEPVAVEERRADAVQTLAERRGVRCDVVTAEGGSALERLASLVAVPDFASIYLALAHGLDPMAVPAINEMKELSNPMPEGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRP8</accession>
    <name>U5VRP8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Collagen alpha-6(VI) chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00580"/>
    <dbReference type="EnsemblBacteria" id="AGZ38405">
        <property type="protein sequence ID" value="AGZ38405"/>
        <property type="gene ID" value="AFR_00580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.118"/>
    <dbReference type="eggNOG" id="ENOG502ZAA0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1025390_0_0_11"/>
    <dbReference type="OrthoDB" id="2092529at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0176">Collagen</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="26596" checksum="9C7289C04BE8E8B6" modified="2014-01-22" version="1">MQPPIAAAAPTTEPRRPVSVAFASILLIVMAVAGLAYAVATLAVAPGTVDRFRAAAGSGSADVDGYVTVVWIVAAFSVVLAVILFALFIVLALGLRRGSNAARIATWVVVGLGLVAGCGTTISMAVQRGGDDAQGTLGAALADAYPASWLGLNVVVSVAQVLGYLLVGVLLLTSPGTFFGRAPKALPPDPFAAPAPGGYPPPPAGYGLPPSAGYGPPAPGGWSAPPAGGYGSPYPSAPPAAGQPIPPGQPASPPQPGPDDEYWSRPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRQ1</accession>
    <name>U5VRQ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00235"/>
    <dbReference type="EnsemblBacteria" id="AGZ38336">
        <property type="protein sequence ID" value="AGZ38336"/>
        <property type="gene ID" value="AFR_00235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.48"/>
    <dbReference type="eggNOG" id="COG1426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055817_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043917">
        <property type="entry name" value="DUF5753"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19054">
        <property type="entry name" value="DUF5753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="29525" checksum="9F106512EAAA8B4B" modified="2014-01-22" version="1">MTIDVVAERLGCSPSKVSRIETGHTSATPRDVKDMLDIYGVDGVESDELVQISREARQKGWWHPFSTVLSGAFVGMEAAAKTVRAYEQQVVPGLLQTDKYAIAMIRAARPSDSDQEIEQRVRVRMARQALLIQDDPIDLQVVLDEAVVSRPVGGDEVMRDQMLRLVEMSLLPNVTLQILPFAAGAHAGMDGTFSILDFPDAEDPNVVFAENATGGLFLEKSDELRKYNSIFETIRATALSPEESRNMIVLLAEEPLWKLRPRVSGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRZ6</accession>
    <name>U5VRZ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359284.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07320"/>
    <dbReference type="EnsemblBacteria" id="AGZ39753">
        <property type="protein sequence ID" value="AGZ39753"/>
        <property type="gene ID" value="AFR_07320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1494"/>
    <dbReference type="eggNOG" id="ENOG5031T1V">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193475_0_0_11"/>
    <dbReference type="OrthoDB" id="2047370at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="68" mass="6976" checksum="085B9F77169BFA78" modified="2014-01-22" version="1">MSSNDALAALRAAGSALGDVDVSGWDESALTDKLAELSDALCQLDAQVSRVADAVRARGYRIAEPVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSC9</accession>
    <name>U5VSC9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferric uptake regulation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359434.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08070"/>
    <dbReference type="EnsemblBacteria" id="AGZ39903">
        <property type="protein sequence ID" value="AGZ39903"/>
        <property type="gene ID" value="AFR_08070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1647"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_5_0_11"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="135" mass="14731" checksum="6687F88438BBF1A9" modified="2014-01-22" version="1">MTDETTVVRNTKQRSAVSAALAEAEGFNSAQDLHAMLRDRGERVGLTTVYRTLQGLADAGEIDVMRPPGGEHLYRRCSQGHHHHLVCRSCGRAVEVEGPAVETWAEKVAGKHGYVDVSHTMEIFGTCPECAAKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSR7</accession>
    <name>U5VSR7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357703.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02355"/>
    <dbReference type="EnsemblBacteria" id="AGZ38760">
        <property type="protein sequence ID" value="AGZ38760"/>
        <property type="gene ID" value="AFR_02355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.476"/>
    <dbReference type="eggNOG" id="ENOG502Z890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043939_1_0_11"/>
    <dbReference type="OrthoDB" id="811477at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1996" evidence="1">
        <location>
            <begin position="77"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="32326" checksum="FCD172633D412D47" modified="2014-01-22" version="1">MAYAAGGRRRALSWALAIELLLAGVAIGAGVLRHDPLGPDYVNIADVTARPAVAGTGSFTWDCGRNERGHRNTANVVVTPGKAGPAHHVHDYVGNLSVDVGSTVKSLAEGNTSCANGDRSTYYWPVLRLAGGAVQVPVSLTLTYYGNVRSPVVEMPRLLRGAVGDAYAFTNGGARAEPVWSCSGSPDRRTTRYPICPGGERVLRVFDFPSCWDGRRTDSSDHRAHLVFPQPGGGCPISTFAVPRLEIVVAYAIGKGTRYRIDAFHAQRHSPLTDHAFFVNLMPGALMREIVRCLNTGQICQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT53</accession>
    <name>U5VT53_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable cytosol aminopeptidase</fullName>
            <ecNumber evidence="8">3.4.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucine aminopeptidase</fullName>
            <shortName evidence="8">LAP</shortName>
            <ecNumber evidence="8">3.4.11.10</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Leucyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pepA</name>
        <name evidence="10" type="ORF">AFR_09270</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.</text>
            <dbReference type="EC" id="3.4.11.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.1" evidence="8"/>
    <dbReference type="EC" id="3.4.11.10" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359732.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09270"/>
    <dbReference type="EnsemblBacteria" id="AGZ40143">
        <property type="protein sequence ID" value="AGZ40143"/>
        <property type="gene ID" value="AFR_09270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1888"/>
    <dbReference type="eggNOG" id="COG0260">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013734_2_2_11"/>
    <dbReference type="OrthoDB" id="356206at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00181">
        <property type="entry name" value="Cytosol_peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023042">
        <property type="entry name" value="Peptidase_M17_leu_NH2_pept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="PTHR11963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTOSOL_AP" evidence="9">
        <location>
            <begin position="358"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="516" mass="52460" checksum="49037C47DC319333" modified="2014-01-22" version="1">MTHPAPSLSLVDSDPAELAVDAIVIGLHSQPDEGGALLPAAGAESIAAAFDGKLTSTLALLGATGAPGEVTKLATLGTIAAPLVVAVGLGDEPSGSAPATETLRRGTGAAVRALAGSGTVALALPVPDDADAPGVLRTILEGALLGSYRFAGYKTKPQPGRRDPVKALQVVVADAADAAASSEVERAGIVSKVVRLTRDWVNTAPNDLRPPQFADAVVAAADGTGLEVEVLDFDQLKAGGYGGIVAVGQGSEAPPRLVKLTYTPQGVENPKRVALVGKGITFDTGGISIKPTAGMWEMKSDMAGAAAVGATMLAVAALKPPVAVTGYLAMAENMPSGTAYRPGDVITMFNGKRVEVLNTDAEGRMVLGDAIARACADGTDYVFETSTLTGGQVVALGKRIAGVMGSPEACERVKVAGDSVGEPAWPMPLPDDVRKGMDSEIADISQVNASMDRAGHMLQGGVFLREFVAEGVEWAHIDIAGPSYHSGEATGYWTKGGTGVPVRTLLTLIDDVAANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT58</accession>
    <name>U5VT58_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359528.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08540"/>
    <dbReference type="EnsemblBacteria" id="AGZ39997">
        <property type="protein sequence ID" value="AGZ39997"/>
        <property type="gene ID" value="AFR_08540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1737"/>
    <dbReference type="eggNOG" id="ENOG502ZY4N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037015_0_1_11"/>
    <dbReference type="OrthoDB" id="1939201at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666049" evidence="2">
        <location>
            <begin position="29"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="443" mass="46285" checksum="72599A11DE008777" modified="2014-01-22" version="1">MKSRRWMRFALPFVVVAGLATLTGIVHAVEQSDPTDASFLSPTSDEGEGARRLADSLARDGVDIDVRSSTADALTAAGAGGPATVFVTTPGLVNPSYLDQFQSLPPEVRVVLVAPGAEQVRRAGLTIPVGGPRWTAAAPQPGCSTDYATAAGPAAALRLAYDPGGYRAVRCYQDGLVEIQTPRARMTLIGASDPFRNDRADEHGNHTLAVGLLGRAPRVIWLDLHERESDPAPTATPPPAPTRDDQSGTDESEPREGEEDEAGEPTDGPSGEPEDGQAAENGGGNAVTGSPLAQAFPPAVWATLALLALAALALAAASARRLGAPVAEPLPVRVRAAETVRGLGGLYRRAGARSTSLATVQNAARLRLTEHFGMPPDSPVDEVAERVAAVTRQPVAEVRHVLGAGVEDSDEELARAATTVQNLVRYVTGQQNWQQGPDEGKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTG1</accession>
    <name>U5VTG1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_03310</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357894.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03310"/>
    <dbReference type="EnsemblBacteria" id="AGZ38951">
        <property type="protein sequence ID" value="AGZ38951"/>
        <property type="gene ID" value="AFR_03310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.666"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_113_1_11"/>
    <dbReference type="OrthoDB" id="1293707at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038318">
        <property type="entry name" value="KdpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025201">
        <property type="entry name" value="KdpD_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003852">
        <property type="entry name" value="Sig_transdc_His_kinase_KdpD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13493">
        <property type="entry name" value="DUF4118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02702">
        <property type="entry name" value="KdpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="604"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ38951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="816" mass="86409" checksum="842BFB401F0B50FA" modified="2014-01-22" version="1">MPRGRLRIYLGAAPGVGKTYTMLGEAHRRAQRGTDVVVALVETHDRTHTTAMIDGLEVLAPTPRGELDLDAVLARKPELAVVDELAHTNGPGSRHAQRWEDVDELLDAGIDVLSTVNVQHLESLNDVVAQITGVAQRETVPDAVVRAAEQVELVDMTPEALRRRVAHGNVYGPDEIDAALGNYFRAGNLTALRELALLWLADTVEDQLGRYRAEQDISATWETRERVVVALTGGPEGDTLVRRAARIANRTRGADLQAVHVMRNDGLTGADPAVLARQRVLVENLGGSYHQVAGPDIARALLDFARAVNATQIVLGASSRGRFAQLLTAGVGVTTTALAGAIDVHLVPHERAGKGTRAQRMPAALSRGRRLAGLALTLAGLPALTGLLLTLAPSLTTDILLFLMVVVAVALIGGLWPALLAAVAGSLLLNWFFTPPYGRFLIAEGDNLLALVIFVVVAVAVSWVVDQAARKTRLAAEAGADAQTLATVAGSVLRGTRPLPALLERLRETFQLTSVTLLERGEVVANVGDSACTDVAGADGAVQVDDELVMLLCGRSPAASDRRIVEAFAAQAAVALRQERLAAEAATARPLAETDRMRTALLAAVSHDLRTPLASAKAAVASLRSTEVHFDEDDRSELLETADESLERLDRLVTNLLDMSRLQAGALGVTIIGVGAEEVVPRVLDDLGPDGRAVITRIPDDLPALLTDPGLLERVLVNLVGNALRYSPADRPPVITASALGDRIELRVIDHGPGIPPDRHDDVFLPFQRLGDRDNHSGVGLGLALSRGLAEAMGGTVTPEETPGGGLTMVVTLPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTG8</accession>
    <name>U5VTG8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="4">3.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">S-adenosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="4">AdoHcyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ahcY</name>
        <name evidence="9" type="ORF">AFR_03335</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>H2O + S-adenosyl-L-homocysteine = adenosine + L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.3.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 6">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="4 6">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 6">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.3.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357899.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03335"/>
    <dbReference type="EnsemblBacteria" id="AGZ38956">
        <property type="protein sequence ID" value="AGZ38956"/>
        <property type="gene ID" value="AFR_03335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.671"/>
    <dbReference type="eggNOG" id="COG0499">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025194_2_0_11"/>
    <dbReference type="OrthoDB" id="522981at2"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="PTHR23420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AdoHcyase_NAD" evidence="8">
        <location>
            <begin position="244"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="273"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ38956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="486" mass="53076" checksum="225044758EB6AA2A" modified="2014-01-22" version="1">MSDKLQQRNGLDFAVADLSLASFGRHQMRLAEHEMPGLMSLRKEYEGQFPLRGARVAGSLHMTIQTAVLIETLVALGADVRWVSCNIFSTQDEAAAAIVVGPEGTVEAPTGTPVFAWKGETLEEYWWCTEQLFNFADGQGPNMILDDGGDATLLVHKGVEFEAAGAVPQATPEDHHEYTIILDTLRRSLASDPQRFTRIAADTRGVTEETTTGVARLYKLAKEGALLFPAINVNDAVTKSKFDNKYGIRHSLVDGLNRATDVMLGGKLAVVCGYGDVGKGSAETLRGQGARVVVTEIDPICALQAAMDGMQVVRIEDVVSEADIFITTTGGQDIITPEHMAAMKHNAIVGNVGHFDDEIDMAGLAKVPGIEKVEVKPQVHEWRFPDGHAVLVLSEGRLMNLGNATGHPSFVMSNSFTNQVMAQIELWTKPGEYEKQVFVLPKHLDEKVARLHLDALGVRLTELTKKQAEYLGVAVEGPYKPDHYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTP8</accession>
    <name>U5VTP8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Octopine transport system permease protein occM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_10420</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360186.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10420"/>
    <dbReference type="EnsemblBacteria" id="AGZ40373">
        <property type="protein sequence ID" value="AGZ40373"/>
        <property type="gene ID" value="AFR_10420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2123"/>
    <dbReference type="eggNOG" id="COG0765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_1_2_11"/>
    <dbReference type="OrthoDB" id="1523519at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="75"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="333" mass="36260" checksum="5E7DB0A8A12B3A9A" modified="2014-01-22" version="1">MTTETQSGRARPEAIKAVPVRHPGRWVTIAVLAVLAAMFIHLLVTNDRFNWRFIFVSIGDGKRGVMFTGPVLEGLRGTILLTVLAMIIGVVLGVVVAVMRLSTNPILSGVAWLYTWIFRAVPRLVLAILFGNLGILWERIGFGLPFDRQIGNLFGIHDLNAQFYSIKATDLLSGFLAGVLALGLSEAAYMAEIVRAGIQSVDEGQAEAASALGLSRGQVLRRIVLPQAMRVIVPPTGNETIAMVKDTSLVAFVPVTFELFFQLQAISSRTFIVLPVLIAACIWYLILSSVLMIVQFFVERHFGRGYGTAGRARLRLRGLTAEQGGTIAEGDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTX0</accession>
    <name>U5VTX0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase GCN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359958.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09840"/>
    <dbReference type="EnsemblBacteria" id="AGZ40257">
        <property type="protein sequence ID" value="AGZ40257"/>
        <property type="gene ID" value="AFR_09840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2006"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091690_0_0_11"/>
    <dbReference type="OrthoDB" id="1983082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="193" mass="21525" checksum="E732A602FFD2F082" modified="2014-01-22" version="1">MRLVPWQPDDMLRRLDDVVSVYGEAMGYRAELLQTRRGYIGAHVRRAGFRAVATLTTDGHLAGFGYGYASGPGQWWHDQVRSSLDEPARRHWLGHCFEVVELHVRPSAQGHGIGARQLRALLSMADGSTVLLSTPEADEQKSRAWRLYRRFGFEDVLRDFIFPGDERAFAILGRGLPLAGRPATEDAPGIVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUS1</accession>
    <name>U5VUS1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11500"/>
    <dbReference type="EnsemblBacteria" id="AGZ40589">
        <property type="protein sequence ID" value="AGZ40589"/>
        <property type="gene ID" value="AFR_11500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2344"/>
    <dbReference type="eggNOG" id="COG1936">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_731349_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="363" mass="37588" checksum="FB94FA11DEC92ADD" modified="2014-01-22" version="1">MIPVLWVCGPPGVGKTSVGWEIYTRLSGSGVACGYVDIDQLGIVDRDPPFDPGRHRLKARNLAAVISGYAAAGARCVIVSGIVDPDHGVHTDDLAGLALTMCVLRADRDELNRRYQGREGADATAPSVRQAVAGPSIEVDTTGPTVAAVAGKILERTGWPRVPDAVGVPGRAGSDPAGAGAEPGGPQGRPEAVSEPAEDGLEGPVLWVCGPTGVGKSSAGFTVFLRTPRAAFVDLDQLGFCAPVPIAQADRLRILGAIWRNYRAAGAQALVLVGPLEGATTATVARLHAGPTQLTERILRRGRGGGSWPQPGDPLLGRPETELRRLAAEAARDAPLTGDSIETDGRSVEEVAGLILARTGWPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUZ4</accession>
    <name>U5VUZ4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360738.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11950"/>
    <dbReference type="EnsemblBacteria" id="AGZ40679">
        <property type="protein sequence ID" value="AGZ40679"/>
        <property type="gene ID" value="AFR_11950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2433"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051715_3_1_11"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="5"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="28430" checksum="C5D5AB31B92D582A" modified="2014-01-22" version="1">MTTPVIFIHGLWLHASSWQPWVDLFTERGYDASAPGWPGDAETVEQTRADPEALAGRGIDDVVEHYAAIFATLHAKPIVIGHSFGGMIAQKLLGLDLAAAAVAIDAAQIKGVLPLPLSTLRSGFPVLSNPANSGRAVSLTPEQFRYAFGNAISEQESRELFDRFSIPAPGKPLFEAAAANFNPHSPAKVETGNESRGPLLLMAGGKDHTVPENVVRATLKQYRHSHAVTDIVDFPDRAHSLTIDAGWREVAETALTWLKEQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV59</accession>
    <name>U5VV59_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative RpiR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06430"/>
    <dbReference type="EnsemblBacteria" id="AGZ39575">
        <property type="protein sequence ID" value="AGZ39575"/>
        <property type="gene ID" value="AFR_06430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1304"/>
    <dbReference type="eggNOG" id="COG1737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055769_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05013">
        <property type="entry name" value="SIS_RpiR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000281">
        <property type="entry name" value="HTH_RpiR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035472">
        <property type="entry name" value="RpiR-like_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01418">
        <property type="entry name" value="HTH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51071">
        <property type="entry name" value="HTH_RPIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH rpiR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="271" mass="28638" checksum="D7647E45B99552E9" modified="2014-01-22" version="1">MLDLFQGARLTPTQRRIAHSLVQHASSAAFLSAAEVAELAGVSQPSVTRFAMALGYAGYPALRRELRALVDVGPAPADSGDNAMQRALRAETQHLERMADQLGSLDAVREAAGLLAASRPLPVLGLRAAGPLAGYFGYFAAKVHPDVRVLDGGGTMLLDRVDQARAAGATAMLAIVLPRYPRETLDVIREAGAAGLRVVALTDSAVSPVAGHADVVLPAPVGTQLVFDLHTGPMAMAMVLLQAMCDAAPEQAQRRLEEFEAYASRRHLFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV73</accession>
    <name>U5VV73_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360761.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12080"/>
    <dbReference type="EnsemblBacteria" id="AGZ40702">
        <property type="protein sequence ID" value="AGZ40702"/>
        <property type="gene ID" value="AFR_12080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2459"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051820_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="51"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="37157" checksum="2C2E1F99467A0B48" modified="2014-01-22" version="1">MYEHREVLERLGALTDAPASHHPADQPDLRDGSVYVCPPRLKTALRVALVTGRPLLLLGHPGSGKSSIAAYVARNLEWRYYEFVVTARAEARDLLYRFDLVRRLADAQVKPGLQLHDIDYVEPGPLWWAINTRSARERGAGDDDRGPSRPADDPFIEINRERRETGAVLLIDELDKADPDVPGGLLVPLGSMRFPVPETGAQVAPLTDPGPLRSRLVIITSNEERELPSAFVRRCIVHRLELPDADGMIEIAQRHLRGRRLRNDQRTRIRDLAHKVVEARPADPAPDEHVPSVAEFLDAVHAMLDLGDMLSDTDWELLEELTITKPSRERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV83</accession>
    <name>U5VV83_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360977.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13160"/>
    <dbReference type="EnsemblBacteria" id="AGZ40918">
        <property type="protein sequence ID" value="AGZ40918"/>
        <property type="gene ID" value="AFR_13160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2672"/>
    <dbReference type="eggNOG" id="COG3463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029114_0_0_11"/>
    <dbReference type="OrthoDB" id="1385011at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018650">
        <property type="entry name" value="STSV1_Orf64"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09852">
        <property type="entry name" value="DUF2079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="465" mass="49475" checksum="BDADD62693270CF0" modified="2014-01-22" version="1">MPAAVVHARTVTTKSTTAPYLLAAALFLAYACWSIHRHLRLQTSGYDLGIFEQAVRGYAGLGAPVSTIKAPGFNLLGDHFHPVLAVLAPLYRLFPGPVTLLVAQAALIAASSIPITRLAIRLAGRSGGLSAGTAYGMSWGLQSAVDFDFHEVAFAVPLLAFGLTALVERRWTAAAAWTLPLIAVKEDLPATVAVIGLLLALRGRRRLGAAMILIAVGSGLVIIGVVLPALNPDHLYPYAGSTAFNGQGPLYRLFFPAIKLHTLLWLLAPTLFLALRSPLLLAVVPTLAWRFWSTNPSYWGTGFQYSAVLMPIVFVAFLDALDTIGNGRAARAAAHATCLIALAATLLNPLPLRGLLMPGTWATDTGAGQVRLALARIPDDARVAADNRFAPQLTSRCTVYFFPVYPQAGVRPEWVAVSDPPDTTMAAPATMAAAIDRLPAYGYQVVLRTSDVIIFHRSGAATVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV86</accession>
    <name>U5VV86_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12155"/>
    <dbReference type="EnsemblBacteria" id="AGZ40717">
        <property type="protein sequence ID" value="AGZ40717"/>
        <property type="gene ID" value="AFR_12155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2472"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_2_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="66" mass="8120" checksum="F40BF6284CAA907B" modified="2014-01-22" version="1">MLPSMDPQRGGRWRDHRQVINGILWRTENGAKWHQVPDRYGPWKTCYHRFSQWEQDGTWARIEKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVA7</accession>
    <name>U5VVA7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alcohol dehydrogenase superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12615"/>
    <dbReference type="EnsemblBacteria" id="AGZ40809">
        <property type="protein sequence ID" value="AGZ40809"/>
        <property type="gene ID" value="AFR_12615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2562"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="2">
        <location>
            <begin position="18"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="350" mass="35702" checksum="AC794EF1BA33E1C8" modified="2014-01-22" version="1">MGSSTMNRWTLTPGAGGGHELTLQTAPVPEPGPGQIRVKVHAAAMNYRDALVRDGHYGSTAASELVPLSDGAGVVDAVGAGVETWTAGDRVISVYFQGWADGAPGPGKGLGLGSGTENGMLAEYAILSADRVTAAPATLSLTEAATLPCAGLTAWTALLGNRPYNARQLDADDTVLVLGTGGVSLFAVQLARAFGAQVWATTSDAGKRERLSGLGVAGVVDYTDVPSWGEHVFAATGGAQIVVNSAGGRSMDQSIAAVAFGGEIAYMGLFDFADVSPDLLTLMSKTASIRGVAVGSVAAQNDLVAAVDQWGVRPVIDEVVPFVDAPSAYERHLSKDSFGKVVIQVDAAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVC1</accession>
    <name>U5VVC1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360883.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12690"/>
    <dbReference type="EnsemblBacteria" id="AGZ40824">
        <property type="protein sequence ID" value="AGZ40824"/>
        <property type="gene ID" value="AFR_12690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2577"/>
    <dbReference type="eggNOG" id="COG1574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028036_0_0_11"/>
    <dbReference type="OrthoDB" id="361195at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010839">
        <property type="entry name" value="AtuA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07287">
        <property type="entry name" value="AtuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="456" mass="48262" checksum="8448B84F3536913C" modified="2014-01-22" version="1">MTHEVRVLTPVGMLGAGFPPETLTRGLELGADVIAVDGGSTDSGPYYLGSGIAKTAAAAVTRDMRLLLRAAATARIPLIIGSCGTSGTDSGVDWVAGITREILAEEGLNLRVATIYSEQQATGLKEHLGQGRIHPLPPAAELDPATLESCRHIVAMMGHEPIVAALEGGADVILAGRATDTAVAAAFPLLKGMPAGPTWHAAKIVECGGQCTTNPRAGGVLATIDATGFTIEPLHPDNACTPTSVAAHMLYETVNPFSMREPAGTIVVTDATYHALDDRRVRVEGSRFDLAEQYTVKLEGARITGYETMSFTAIRDPHILSQIDTWAALLRRMIGEWVTDTLGLTSDEYAFDIRLYGHNAVLDAIEPATGPPREVGVMLLVNAADQQTATAVAKVANPLMLHLPTPDMNYLPSLAFATSPAETERGAAYEFVLNHVVDVDSPTAPFRINLPEDTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVC2</accession>
    <name>U5VVC2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Epoxide hydrolase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_12355</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="U5VVC2"/>
    <dbReference type="STRING" id="1246995.AFR_12355"/>
    <dbReference type="MEROPS" id="S33.971"/>
    <dbReference type="EnsemblBacteria" id="AGZ40757">
        <property type="protein sequence ID" value="AGZ40757"/>
        <property type="gene ID" value="AFR_12355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2508"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019414_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EHN" evidence="5">
        <location>
            <begin position="4"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="205"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="373" mass="42369" checksum="9D73ADA818FAB985" modified="2014-01-22" version="1">MIRPRPFAVSDDALDDLRERLRRTRWPEPEPVQDWSQGVPSRRLRALCAYWRDSYDWRACEARLNALDPSTTHIDGLDIHFLHLRSPEPDATPLVMTHGWPGSVIEFLKVAPLLADPRAHGGAAEDAFHVVLPSLPGFGLSGKPAETGWGPERIARAWVELMQRLGYHQFVAQGGDWGHAVTTALGGIGEPAVRAIHSNMFPTYGAEEATSPPERRALQRQRDFEDNESGYKHEQIQSPQTIGYALTDSPVGQAAWIYEKYQQWTDHGGDPEALLSRDEMLDNISLYWLTGTAASSARIYWESFRGYRDPKVDVPVGVSIFPRDIYLGSRRWAEAKYPRLVHYQEMPRGGHFAAWEQPELLAEEIRLTFRHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVC7</accession>
    <name>U5VVC7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Imidazolonepropionase</fullName>
            <ecNumber evidence="1 4">3.5.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Imidazolone-5-propionate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hutI</name>
        <name evidence="6" type="ORF">AFR_06410</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the hydrolytic cleavage of the carbon-nitrogen bond in imidazolone-5-propanoate to yield N-formimidoyl-L-glutamate. It is the third step in the universal histidine degradation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>4-imidazolone-5-propanoate + H2O = N-formimidoyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23660"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
            <dbReference type="EC" id="3.5.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="4">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc or iron ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. HutI family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.7" evidence="1 4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359102.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06410"/>
    <dbReference type="EnsemblBacteria" id="AGZ39571">
        <property type="protein sequence ID" value="AGZ39571"/>
        <property type="gene ID" value="AFR_06410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1300"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041647_1_0_11"/>
    <dbReference type="OrthoDB" id="785930at2"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00551"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050480">
        <property type="term" value="F:imidazolonepropionase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00372">
        <property type="entry name" value="HutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005920">
        <property type="entry name" value="HutI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752">
        <property type="entry name" value="PTHR42752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01224">
        <property type="entry name" value="hutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="5">
        <location>
            <begin position="56"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>N-formimidoyl-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>N-formimidoyl-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="382" mass="39852" checksum="4605192B51A27BF0" modified="2014-01-22" version="1">MSLLITNIGELVTNDPELGVLSDAAVVADGDRVAWVGPAAAAPAADERLDARGAAVIPGFVDSHAHLVFAGDRAGEFAARMNGEPYTGGGIRTTVAATRAATDDELLASAQRLVREMRRQGTTTVEIKSGYGLSVEDEVRSLRVARQLTAETTFLGAHVVPAEYTDDPDGYVRLVSGPMLEAVTPYARWVDVFCDRGAFDGDQARTILTAGAQAGLQLRVHANQLVAGPGVQLAVELGAASADHCTHLSDADVAALAGSDTVATLLPGAEFSTRSPYPDARRLLDAGVTVALATDCNPGSSYTSSMAFCLALAVREMRMTPAEALWAATAGGARALRRDDVGVVRPGARADLVVLDAPSYLHLAYRPGVPLITTTLHDGVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVR7</accession>
    <name>U5VVR7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SMI1_KNR4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361028.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40969">
        <property type="protein sequence ID" value="AGZ40969"/>
        <property type="gene ID" value="AFR_13415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2724"/>
    <dbReference type="eggNOG" id="ENOG5033Y5V">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1793925_0_0_11"/>
    <dbReference type="OrthoDB" id="1941876at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09346">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SSF160631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMI1_KNR4" evidence="1">
        <location>
            <begin position="30"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="164" mass="18316" checksum="16F587BAD71BDC94" modified="2014-01-22" version="1">MTEDEIFDAVAARVESGHQADVPDAYPQEPATEEAVADAERIIGYPLPPLLRRLYLEVANGGFGPFNGIEGVADGYAAEEGMLENYLSWRVEDLPDDFPAWTPGVVAFCDFGCAMWALLDCRTPEGRVQFLDQSTLHRLDLPLARWFELWLADDNLDMHKLITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWA6</accession>
    <name>U5VWA6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA_16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_08285</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08285"/>
    <dbReference type="EnsemblBacteria" id="AGZ39946">
        <property type="protein sequence ID" value="AGZ39946"/>
        <property type="gene ID" value="AFR_08285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1689"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002352_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="G-protein_beta_WD-40_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="SSF50978"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="AAA_16" evidence="4">
        <location>
            <begin position="52"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="541"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="584"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="626"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="667"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="709"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="751"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="793"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="877"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="919"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="961"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1003"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1045"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1087"/>
            <end position="1119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1151" mass="125822" checksum="EF258DD514FF7401" modified="2014-01-22" version="1">MDLSGGTEKGWEHLWQSLMRLGLDPLDDFPWDPQRPPYPGLSAFQESDSAVFFGRTTEVDNLLNCFQQRLSPSRQGVAIIGPSGSGKSSLLRAGLLPRLRKMSTEWIIVPPFRPTDGETPLSALARRINGLVPPSDQQGWRATSDLLNAEPTALLEISRDLIDYNGKADACVVIVIDQLEELITRASPDQRTRFGEAILAATYSGSPVWVVTTLRSEFLSRFLETPRFAEVVQRQILVGPMTSGRLQEVIEEPARKTGLTFDEGLKERMVEDTTGGDALPLLAYTLRRLYDQVQGRQVRRITDADYMAIGGRYAQAGGVIGVLMTYADQIVESLGPDGTLVVPTLLKMVNLDAHNQPTKRRVSRTEFRNGEERIVEAFVQQRLLTTDRADNGEVMVEVAHEALLRVWSPLETAINAARSDLLARSELERAARDWHVNKRRPSYGLQGERLVVADRLVSRGLVNLDHENLLADFLRASRRADAATRQRNSEQLAERVQGNLNEDPELSILLALAAIEDYGPTARAILALNSAVDKSRLRHSIHGHDGPMLSARYSPDGSRIVTSGEDGKARVWLLADTSEPETVFGAHTGIVGSADFSPDGRTVVSGSEDCTARVWSVGTGAEILRLTGHSGRVRHVAFCPCSEHVITASEDHTAKVWNMSGSVLSTFEGHTGIVLATDVSPDESLLVTASSDHSARVWDLWTGEQLRTLIGHEDWLRSPHFSPDGVRIATASEDGTARVWNASDGSCTEVLNGHSGRVRASVWSPTGDRLLTGSGDGTARVWDSKDFSEKVALRGHKNRVRDADFSPDGIHVVTASEDGIARVWTVEENPDQFVVARYRTRVRSACWSPSGSSLAIAKEDGSAEVVVLGELARRIKLEGHRTRVAKSIFSPDGTTVLTGSSDGTARVWDTASGECLMTIEAHADWIRDIALSHDGERLATASSDKSVSMWSLTTGEKIAELIGHQAPVLSVTFSPDGLHILTASADRTAKIWSIASSIEVLSLDGHGDWVRSANYSPLGDQVVTASEDATARVWDPSTGDSILVMDAHEDWVRSASFAPDGIRIVSASGDRTVRVWRATNGEQLHIFRQHTEMVGSATYDASGTRIVTASHDGTVRIFEDLSLDGLLSKARRRTFRNLTPEERAGYGLEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX77</accession>
    <name>U5VX77_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HtpX-like protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_10205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360102.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10205"/>
    <dbReference type="EnsemblBacteria" id="AGZ40330">
        <property type="protein sequence ID" value="AGZ40330"/>
        <property type="gene ID" value="AFR_10205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2080"/>
    <dbReference type="eggNOG" id="COG3212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891680_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025711">
        <property type="entry name" value="PepSY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03413">
        <property type="entry name" value="PepSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666170" evidence="2">
        <location>
            <begin position="27"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="PepSY" evidence="3">
        <location>
            <begin position="69"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="134" mass="13710" checksum="CA4434D8DFDDBCF5" modified="2014-01-22" version="1">MIRLRTVTALAAGTVAALAIGGTALAAGTDDPPGVEMLPDGDVVAAPSPSSFPSSTAPTREPSPSSSAAISLGDAKAIAIKAAGGGRVTDVERETEHGRAVWDIEVQKGAIEHDIDVDRTTGDVLRHRSERDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXG8</accession>
    <name>U5VXG8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_10705</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360299.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10705"/>
    <dbReference type="EnsemblBacteria" id="AGZ40430">
        <property type="protein sequence ID" value="AGZ40430"/>
        <property type="gene ID" value="AFR_10705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2182"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_11"/>
    <dbReference type="OrthoDB" id="972769at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ADH_N" evidence="4">
        <location>
            <begin position="25"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="ADH_zinc_N" evidence="3">
        <location>
            <begin position="178"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="335" mass="34300" checksum="4FF391FC8B4572CA" modified="2014-01-22" version="1">MRAFVITGPGKAEVQDVKPPEARAGEVVVDVARAGVCGTDMEIYSGAMSYLHTGQAQYPMRIGHEWSGTVASVGPGVDEAWLGRRVTGDTMLGCGHCARCTSGRQHVCADRYEIGVRNGWPGALAEQLPVPVKALQVLPDSVDPAAGAMVEPAGNALRAVRGVALPPGGRLLILGPGAIGLLTAQVAAAQGAEVHLLGRSATSLEFAASIGFPRGWTTETLPDLTWDGVIDASNGAALPALAVDLVEPGRRVVFVGLSSSPSLIDSRNLALKDVTAVGVLSASGGFAGTVELFASGEVDPRPLIAATVGLDDVAAVLAGDRSWGAAPKIHVDPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXH3</accession>
    <name>U5VXH3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361572.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16075"/>
    <dbReference type="EnsemblBacteria" id="AGZ41497">
        <property type="protein sequence ID" value="AGZ41497"/>
        <property type="gene ID" value="AFR_16075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3262"/>
    <dbReference type="eggNOG" id="COG1914">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033610_0_0_11"/>
    <dbReference type="OrthoDB" id="416257at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="467" mass="50936" checksum="F0C4226DD572C550" modified="2014-01-22" version="1">MARSQTTDDLDTRSLPEPLSLRRMVGPGVVAVGIGMAAGEIILWPYLTAIGGLGLLWLAFTTLAVQFVINMEIERYTLATGQTVVAGFSRWWKGWGIIICLAGAFQYVWPGWATSGSTVFTYLVGGGDPVWITVASLVIVGVLLSVSKVVYTTVERVEIVKVGLTLFFLLVVVIFVISLSTWGEGARETVTGFGRIPEGITFTLLLSAIGAAGAGGVHNLVLSNWIRDKRYGMGAHVPRLVSPITGREEAAGTTDRYALALDDANLSRWNIWWKRANAEHLVTFVAVCFATITIMCLLAYETLFGRDDLKSDASFLRIEADILADQVGTWLKILFLAVAAVSLWAASMGLLDIIGRVASDFLRRNYLTNSARWTEPRLYLVVVWAEIVLGSLILLAGFTQPLGLLIVSTCAASVVTLLYSVLLVRLNTRDLPAPVRLRGWRLGGMLIAIGFYGFFAIAMLITQLQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXI3</accession>
    <name>U5VXI3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361629.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16360"/>
    <dbReference type="EnsemblBacteria" id="AGZ41554">
        <property type="protein sequence ID" value="AGZ41554"/>
        <property type="gene ID" value="AFR_16360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3318"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_11"/>
    <dbReference type="OrthoDB" id="1582456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="29378" checksum="453A6BABB123192D" modified="2014-01-22" version="1">MTKVWFITGTSKGFGREWAEAALDRGDRVAATARDTTQIKDLTARYGEAVLPIELDVTDRTRAFEAVRTAADHFGRLDVVVNNAGYGHFGMVEELSEDDIRAQMETNFFGALWVTQAALPIMRAQRSGHLIQVTSEGGVIAYPGIGAYHASKWALEGLTQSLYQEVGAFGIHVTDLEPGPYATDWLPGGARHSAENPDYQPVRAGLKSDWDLGDPRATRAAILALVDAEKPPLRLLLGKSLAMVTEDYRSRLQTWNDWQDVSVRAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXJ4</accession>
    <name>U5VXJ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360344.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10830"/>
    <dbReference type="EnsemblBacteria" id="AGZ40455">
        <property type="protein sequence ID" value="AGZ40455"/>
        <property type="gene ID" value="AFR_10830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2207"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_0_11"/>
    <dbReference type="OrthoDB" id="1617768at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="117" mass="13372" checksum="5418FF84C24418A5" modified="2014-01-22" version="1">MHAFDVLGDPVRRRILELLADGEQTSGAISAVVQAEFGLSHPGVSQHLRVLRESGFTTVRAEGTRRLYAVDAEPMREVDVWLDQFRRFWNQRLDALATELARGKRARRRPTTERDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXN3</accession>
    <name>U5VXN3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361950.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17300"/>
    <dbReference type="EnsemblBacteria" id="AGZ41738">
        <property type="protein sequence ID" value="AGZ41738"/>
        <property type="gene ID" value="AFR_17300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3508"/>
    <dbReference type="eggNOG" id="COG3918">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709671_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="13750" checksum="453982BF199E0309" modified="2014-01-22" version="1">MTDHVLLRAAVLGAAAGARSATPLAALAQRRGGPVRILATLAAAGELVVDKLPGTPSRLEPGPLGGRIVLGALAGAVLARRAGAGVVAPALIAAVAAVAASHAGASWRAYASRSGFGTPGALAEDAAAVIAASIAAEGALVHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYG5</accession>
    <name>U5VYG5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360745.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11995"/>
    <dbReference type="EnsemblBacteria" id="AGZ40686">
        <property type="protein sequence ID" value="AGZ40686"/>
        <property type="gene ID" value="AFR_11995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2442"/>
    <dbReference type="eggNOG" id="COG4292">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707713_0_0_11"/>
    <dbReference type="OrthoDB" id="886435at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010640">
        <property type="entry name" value="Low_temperature_requirement_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840">
        <property type="entry name" value="PTHR36840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06772">
        <property type="entry name" value="LtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="412" mass="43725" checksum="2FF351BD38F593F4" modified="2014-01-22" version="1">MVNIDDEPTEARTERHASWAELFFDLVAVAGVAALAHVLGPDLDAAALGLYALMFLALWLSWTTFMLYGNVAAGQTRVLRLMVGMFGLGVMAASVPGVAHTVLDHDGDSLPLNVFAIAYIATRVYGARSWQRGAVLLDFPVVQYSAGLLPWLASLWVDEHWKLVLWAVGVGLDLLLIVVLSGSRMLQRVQAYLAAPERTRRRSRGRSDGSGPVIHGVSVDPVHLAERLGLFVIIVLGESVVQIVAAAGETHFDLGVLAAALASFILLAGMFGLSVVFGHAGLPHLRAGRIPSRAALGLHCLVTGVVATIAVPLSLVVEHGSDPLPDQARWLLCGAVAAYFTLGVVTGVASHSSDRPRTISRITTGIVAPLLLGLLATAAAGRTLVACLALIVLAHLWFERRLAPLGPHIRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYI9</accession>
    <name>U5VYI9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360839.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12470"/>
    <dbReference type="EnsemblBacteria" id="AGZ40780">
        <property type="protein sequence ID" value="AGZ40780"/>
        <property type="gene ID" value="AFR_12470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2533"/>
    <dbReference type="eggNOG" id="ENOG5030HYV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_992598_0_0_11"/>
    <dbReference type="OrthoDB" id="2006760at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="27745" checksum="FEAF66146F6F8B4D" modified="2014-01-22" version="1">MDSSPTLQDFVLNLIYDSAARSAFELDPETTLQHAGLSDVTAADVQQVIPLVVDYAPVAGITDVAGLDDLSTGVANLDVAGAVAQLQTITATAALSPVQGAAGADVNVSLAGVHTTALVGEDLLTGGSVLTGASLSVGGVTTVGGVADLSVDNDPALDLDAGVAVENTVDTVVPGAGQLIPDTDLRPIETLDHVTNVPSSVDLSNVTDLDVAGLHGATSSVVSVVSPDLGDDVVHGVHGTVTGTLDSVDGVTGGVLPSIGHPADNDLGHDLLGGLTDLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYN5</accession>
    <name>U5VYN5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">17.5 kDa class I heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362377.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18630"/>
    <dbReference type="EnsemblBacteria" id="AGZ42004">
        <property type="protein sequence ID" value="AGZ42004"/>
        <property type="gene ID" value="AFR_18630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3781"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_9_0_11"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="23"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="15646" checksum="317B9036B7CDC111" modified="2014-01-22" version="1">MVLTFDPFREFDRLAGQLSGGAGDPAGLAMPIDLYRSGDHFVLHCDLAGADPGSVEVDVDGRVLTIRAERSARTDADVQWLRRERPTGTFTRRLTLGDGLDLDRIDATWQEGVLTLTIPIAEAARPRRIAINTGQASTAPALSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZN3</accession>
    <name>U5VZN3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_20905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20905"/>
    <dbReference type="EnsemblBacteria" id="AGZ42453">
        <property type="protein sequence ID" value="AGZ42453"/>
        <property type="gene ID" value="AFR_20905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4238"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="148"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="222" mass="24188" checksum="F486920653E9B901" modified="2014-01-22" version="1">MTPVRVVLADDQPLLRRSLAMIIDSAPDLTVVGQAADGAEALSVTHSTRPDVVLMDVRMPHVDGLEATRRICTDPALTATRVVVLSMFELDEYVHEALHAGAAGFLLKDARPEDLITAIRRTHDGESLFAPSILTRLIEHYLTGPRSHHRPPAELTARETEVLTLIGGGLSNDQIAARLTISIKTVKTHITHLLTKLTARDRAHLVIAAYDAGLVRPRPFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZS2</accession>
    <name>U5VZS2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative FGGY-family carbohydrate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_14320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361205.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14320"/>
    <dbReference type="EnsemblBacteria" id="AGZ41146">
        <property type="protein sequence ID" value="AGZ41146"/>
        <property type="gene ID" value="AFR_14320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2906"/>
    <dbReference type="eggNOG" id="COG1070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_3_2_11"/>
    <dbReference type="OrthoDB" id="1619686at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="1">
        <location>
            <begin position="18"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="2">
        <location>
            <begin position="299"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="493" mass="52274" checksum="BEF95FBB44168FFC" modified="2014-01-22" version="1">MTRIDDDVALETALDPLVLALDIGSTATRGGVHDASGRRVHGLQHKVPHAFLVAPDGTSVIDPEQVTTEVAQIIDTVTADPRLGSRIAGVAMDTFAASLIAVDADGRALSPCLTYADSRSAGAVTALREELDEHAVQQRTGTRLHTSYHAPRLRWLRTAQPQVVAGAVFWWSLGEYVWARLIGQPLAGTSTVAWTGLLDRRTGELDAELLAAAGVGPEQFSPPRDMTDPAPSVAPGRWPALTRAVWFPVITDGFASNIGAGAIDSTVLTAATATSGALRVLLDGPADPLPFGLWNYRVDAGRTLLGGAINDVGRAVSWAQSTLRLSPDVTSALATPPGEATPLVLPYLTGERAPGWVGEARAVFGGVSAATDADTLFRGIVEGVAMTYARVADELRPAAPQILEVAAAGRVSNDQPDWLQILADVLERPVTHVTRRRATQRGTALLALDVLAPDVPRAPRVTGSTYEPRPAHAGYYAERRARFGEMYDAQIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZY0</accession>
    <name>U5VZY0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycoside hydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_14645</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14645"/>
    <dbReference type="EnsemblBacteria" id="AGZ41211">
        <property type="protein sequence ID" value="AGZ41211"/>
        <property type="gene ID" value="AFR_14645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2974"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_419617_0_0_11"/>
    <dbReference type="OrthoDB" id="1226595at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02850">
        <property type="entry name" value="E_set_Cellulase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004197">
        <property type="entry name" value="Cellulase_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02927">
        <property type="entry name" value="CelD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CelD_N" evidence="5">
        <location>
            <begin position="42"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="626" mass="66276" checksum="693B46F95A83A610" modified="2014-01-22" version="1">MVAMLTATVLAAVACDSPPPHEVVLPSREAVQPTPPAVLPEQDGALRVDQVGYVTGETKIATLLAPRDATGARVTVVGPRGETVLPPVGTRRGTWNTGFPSVHLIDLTALTRPGTYRVRVEGPVTAESPPFRIGSAGDLFGPLAGNSLVYFQAHRDGADQVAGAWRRAPAHLTDREATVYSDPGYDDDGRMVTAMEPVGGPVDVEGGWYDAGDYLKFSHTTAYALIAMLLVQRDGPAPAGLADETRHGIAWLDKMWDEDTGTLYTQVGIGSGINPGFLGDHDTWRLPQDDDRLTAEPGEARYYQRYRPVFRAAAAGAPLSPNLAGRVSAAFALAAQVEASTDPARARRHLAAAAQIFALADTSDTGDLVTAQPRSFYPEDSWADDLATGAAELVLAGQALRDPRTSEWARQGEHWAEVNTGTGRTDALNVYDLSAIADAELVKAGVSPGGLLRDLRKRLDAGVRAAGTNPFRAAAGNGGYDYASRELGYVVTAALYRGITGDDRYTDFGTTQRGVVLGANGWGTSLVVAAGTTFPRCPHDQIASLTAGPPAPGMTGAVVNGPNKAERIHELIEQRTSDACSTDSFAAYDRDDAQYTDDEQVSANTEPSIDFTATGMLAFALTARDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W049</accession>
    <name>U5W049_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361414.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15365"/>
    <dbReference type="EnsemblBacteria" id="AGZ41355">
        <property type="protein sequence ID" value="AGZ41355"/>
        <property type="gene ID" value="AFR_15365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3120"/>
    <dbReference type="eggNOG" id="COG5592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079417_2_2_11"/>
    <dbReference type="OrthoDB" id="1701106at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin" evidence="1">
        <location>
            <begin position="9"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="187" mass="21201" checksum="2C3131D252F8B2A2" modified="2014-01-22" version="1">MTSTPAGDVVDVLTSDHRDVTALIAEIWSVRDPMVRRDLTDTAISELVRHAVAEEMYVYPAMRKYLPDGDKSVEHDVEEHKELEETMKRLEDVDVSSTEFDEALRKLETILADHVQDEESEQFPELRRRVPREELVEIAGKVETAKKLAPTRPHPAAPNNELFHKLVGPGVGLVDRLRDKLTGRATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0L0</accession>
    <name>U5W0L0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16215"/>
    <dbReference type="EnsemblBacteria" id="AGZ41525">
        <property type="protein sequence ID" value="AGZ41525"/>
        <property type="gene ID" value="AFR_16215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3290"/>
    <dbReference type="eggNOG" id="COG4213">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_13_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665554" evidence="1">
        <location>
            <begin position="20"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_4" evidence="2">
        <location>
            <begin position="41"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="363" mass="37586" checksum="5A3256FD6099FF86" modified="2014-01-22" version="1">MVALTTAGLLATVGLSACGDSSGDDSGSGSGTSGAGKVGVILPDTKSSARWATADLTYLKEAFAAAGVEADIQNAQGDKTAFQTIADGMISAGVKVLMIVNLDSGTGKAVLDKARKAGIATIDYDRLTLSGNADYYVSFDNVKVGELQGQGLVDCLAEKKYTKPVVAELNGSPTDNNATLFAQGYDSVLDPKYTDGTYLKGPNQDVPDWDNTQAGTIFEQMLTSNKEIKGVLAANDGLGNAAIGVLRKNKLNGQVPVTGQDATVQGLQNILAGDQCMTVYKAIKKEADAASQLAIALAKEQKPTTATGTTKDPESGKDIPSVLLTPVSITKANVKDVIADNYVTKEELCKAEFAKLCADAGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0V7</accession>
    <name>U5W0V7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22520"/>
    <dbReference type="EnsemblBacteria" id="AGZ42774">
        <property type="protein sequence ID" value="AGZ42774"/>
        <property type="gene ID" value="AFR_22520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4565"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079365_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="122"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="29069" checksum="891128C41D949FB1" modified="2014-01-22" version="1">MGSVLLRAYDNEVRPVPPAAREGLVQETDGPVLRTLSARGGRISYVDLGGLTGDELDAFIRRQRDHFVRLDRPVDWITYTHDEPQDLPGRLEAAGFVLCETGTMLAGRCDRLASPVRLSGGLTIREIGTREDFERVAALQGAVWGIDASWLVPQLTGQHTGEPDRWTFLVAESGGEFVSAGWTRYYTDRFAMLYGGATLPGFQGRGIYRALVARRAQYALDRGREFVVVDASESSRPILQRLGLQPFATRVTYRWTPPRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W176</accession>
    <name>U5W176_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361766.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16840"/>
    <dbReference type="EnsemblBacteria" id="AGZ41646">
        <property type="protein sequence ID" value="AGZ41646"/>
        <property type="gene ID" value="AFR_16840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3416"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_0_11"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="135"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="35192" checksum="4FC97A7AFCBFF929" modified="2014-01-22" version="1">MTRAALIGCGDVSVVHLEAIAELPGVELAGVCDTDPGRARAASERYGVPAFTDHHELLTAVRPEVVHVTTPHHQHVDVAIDCLDAGAGILVEKPVAHTVEEADRLVAATRSRPELRAGICLQNRYNTTSRAAHDLLASGELGTVLGASATVLWHREAAYYAARPWRGQVRNSGGGVLINQAIHTLDLLQWLLGDVVGVRGHSGRYLLKDIDVEDTAHAVLEHAGGARSTFFATVTHVTDSPVTIEIVTERAVLLIRGDLTVTYADGRVETVAERPPGSGGRAYWGASHELLIADFYRTLAGPAPFWISPQEGAGSLRLVHRIYHQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W187</accession>
    <name>U5W187_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transporters inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_22535</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363190.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22535"/>
    <dbReference type="EnsemblBacteria" id="AGZ42777">
        <property type="protein sequence ID" value="AGZ42777"/>
        <property type="gene ID" value="AFR_22535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4568"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_11"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="90"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="34575" checksum="39F868F023C09291" modified="2014-01-22" version="1">MALTFAATPRTHQQVAPRPRGRRGRAARRTLTGWAFAGPATLIVVGLSIFPAVWAFFISRTRWNGIAPARDVGWRNYERIATDPDAITAAKHTLLLTALFVPGSIVLGILIAVALNQKIRLIGFYRTCIFVPYVASAAATGILASFVFNPQFGAANEALRRLGLPAQQFLESPGQALLVICLIALWGEVGFTAVIYLAALQDIPRELIEAATVDGASRWRTFLHITLPELRPVTVFAAIWQTITAMQLFDLIYTTTRGGPMNSTQTIVYYIYELAFQTQRLGYGAAVAYLLFVVTMLLTVCVIWYSRRSGSEAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1D2</accession>
    <name>U5W1D2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362024.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41775">
        <property type="protein sequence ID" value="AGZ41775"/>
        <property type="gene ID" value="AFR_17485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3544"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_0_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="30510" checksum="338ABA7723A816DF" modified="2014-01-22" version="1">MTQTSPFTARTTAQEILAGADLTGRRILVTGGGSGLGAETVRVLAAAGADVTLGVRDPATAKSLGVPPLDLADLDSVAAFARWWRGPLHALVANAGVMAVPERRLTAQGWELQLATNHLGHFALATALHDNLREAGAARLVVVSSGAHRDVPFDFADPQFARRPYDRWVAYGQSKTADVLLAVGAARRWAADGITANAVNPGWIMTGLQRHVDDTTMKAMGAMDEHGVIIEQPYSKNLAEGASTSVLLAGSPLVAGVTGQYFEDNHIGGGAAHALDPEAADRLWELAAAAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1H0</accession>
    <name>U5W1H0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17735"/>
    <dbReference type="EnsemblBacteria" id="AGZ41825">
        <property type="protein sequence ID" value="AGZ41825"/>
        <property type="gene ID" value="AFR_17735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3596"/>
    <dbReference type="HOGENOM" id="CLU_2406680_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="92" mass="10049" checksum="3992FD1960F880FE" modified="2014-01-22" version="1">MTATVGSSTFMRRLRHRFRFLGGGMGSAVSHADMGPYAGAPRDPELRIGDEKSYSTIMPTHQVTVVATADDRWQSPMPAIAKAREASRKTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1J3</accession>
    <name>U5W1J3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-keto-4-pentenoate hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23755"/>
    <dbReference type="EnsemblBacteria" id="AGZ43019">
        <property type="protein sequence ID" value="AGZ43019"/>
        <property type="gene ID" value="AFR_23755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4811"/>
    <dbReference type="eggNOG" id="COG3971">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060136_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="92"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="254" mass="26272" checksum="5FE6BBF62D6B5111" modified="2014-01-22" version="1">MEAAARLQEAARTGKPCAPVRDLIGATDAYAVQEVLTEARLAAGARIVGHKIGLTSAAVQRQLGVDQPDFGVLFADMDVSEHAAIPLAGLLQPKVEAEIAFVLSEDLDSEDLTPERVRAAVAYAVPALEIVDSRIEDWDITIADTIADNASSGLFVLGDDRVTLDDAEPKDAVMSMTVNGEVASTGTGAACLGDPLAALAWLARTAREVGRPLRAGQVVLSGALGPMVPLEPGARISATMTGFGTVTATVGELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1N9</accession>
    <name>U5W1N9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_18110</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18110"/>
    <dbReference type="EnsemblBacteria" id="AGZ41900">
        <property type="protein sequence ID" value="AGZ41900"/>
        <property type="gene ID" value="AFR_18110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3675"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_551683_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="277"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="418"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="344"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ41900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="602" mass="62411" checksum="576A85FA25BA1E94" modified="2014-01-22" version="1">MPSLPLRPGDPERLGEYELTARLGAGGMGTVFLGRAPQGRPVAIKVVRSEFVRDEEFLGRFRSEVNRARQVPPFCTAEVLGADLDHDPPYLVVEYVDGPTLTDVIREHGPLSPAGLYSAAIGIATALTAIHGAGVIHRDLKPGNVLFALGGVKVIDFGIARPLEATSAHTRTDQMVGTVAYMAPERFDEAPGPRVSPAADIFAWGAVVTFAGTGRTPFAADSAPATAMRILTQPPSLGDLPMPLRTIVERTLAKEPSERPTARELLDLLLATGSPAALPPRAVPDDPTHVLPQYAAGSTPAGATTAGDGQGLDDPHRPAGQPQAGPVQAGQPQAGAARAGQPQAGQPQPWQPQAGQPHGGQPQGGQPQTWQAPGGQAPGGQAPSGRKGRRKGRVLTTAALALAAAVALGGVVLFSKADSETTGEGRNPATGIGGPSSAPAVSPSASRPAPGPTGNAGVLAGSRKTLIHFVEIDRDLAMPFQDEVIASDGTGKDALFALIPLGVDFLIQSVQPTEGEPPCLGVKIVPDDSAELVATECAGTKATLFSISPTGAKDDKGRPTYTIYNDSYGLVQYYETEKRVYVEQLGDAPPDTTFSFVDRGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1V3</accession>
    <name>U5W1V3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative signaling protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18140"/>
    <dbReference type="EnsemblBacteria" id="AGZ41906">
        <property type="protein sequence ID" value="AGZ41906"/>
        <property type="gene ID" value="AFR_18140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3682"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="336"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="475"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="732" mass="77595" checksum="6B0303A9876D2FFD" modified="2014-01-22" version="1">MLWMGLGTVACLGYPFLPPGGGRAVAAWLFGLVALLVMVAGVRRNRPTDVRPWYVFIAGQVLWVLGDAAYAIDETVRGFHPYPSWSDAFYLSAYPPLIAGLYGLARGRRRREWSGLVDTAIIAAGLGLIYWIFVIGPILGDTASPLVGRLVTIGYPTASVLMIAVVARLVTRAGRPTVSGWLLIIGSVLTLAANVVYTLMPAVLMVAGDFVYTGFLFAYVYWAGAALHPSVSARPRATGREQFGRGRLAILTCSTLVVPAVLFLQGAHVGRPVSWLAIGVGAVVLFVLVLIRMAGFVAQVQAQAGQLEILAMRDELTGLANRRRFEQRVREALETGSPQVAMLDLTGFKAVNDRFGHAVGDELLAAVGARLGSGLRPGDLAARMGGDEFAVLVADATAAEGDTMAARLGAALRVPIETGEHELLIGAGIGVAGADGTDDAVELLRRADVAMYAAKADGDKLRRYSADLDDEAGEEARLGAAVRIALDTGQFRLVYQPIVSLPDGRIVSVEALIRWDHPDRGFVSPAEFIPVAEQNGLIVELGAWILRTACAQAVVWRNTLGDAAPERVSVNVSARQLAEPGFTELVSSVLAWTGLGAQNLILEVTETAVFGGGQAVQAVKDLHELGVRIALDDFGTGHSSLGLLQTVPVDVLKVDKSFVDNITLAGREAVIATALIQVSHGLGLMAVAEGVETAEQAAELHRLGYRLAQGYHFGRPVAEPDFLGEPTAVAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1V9</accession>
    <name>U5W1V9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_24710</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24710"/>
    <dbReference type="EnsemblBacteria" id="AGZ43208">
        <property type="protein sequence ID" value="AGZ43208"/>
        <property type="gene ID" value="AFR_24710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5006"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="36"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="125"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="20705" checksum="76DAEB75484E886F" modified="2014-01-22" version="1">MENRVEDAAAVTRARDGDLDAYEVLVARYTVPAHRAATLLGAGADADDVVQEAFVKAYRQLGRYRGESGFRPWLLAIVANETRNLHRSRKRRDGLVLRAAAGEQPDPVAPDPADTVLADERRQHLVDQLRLLDVRDREVLVCRFLLDLSEAETAVTLGLPKGTVKSRTSRALTKLRSRMREEVHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1X2</accession>
    <name>U5W1X2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18535</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362358.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="SMR" id="U5W1X2"/>
    <dbReference type="STRING" id="1246995.AFR_18535"/>
    <dbReference type="EnsemblBacteria" id="AGZ41985">
        <property type="protein sequence ID" value="AGZ41985"/>
        <property type="gene ID" value="AFR_18535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3762"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157807_1_0_11"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="4">
        <location>
            <begin position="8"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="3">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="88" mass="9305" checksum="D2C0050C152923C6" modified="2014-01-22" version="1">MSDLSTAPTLDSLRVWLVDCVAGHLGLDAATIATDLPLTSYGLDSVYALSIAAELEDHLDVSLDPTLIWDHPTIDALSTALVAELRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W291</accession>
    <name>U5W291_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">50S ribosomal protein L11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363717.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24305"/>
    <dbReference type="EnsemblBacteria" id="AGZ43127">
        <property type="protein sequence ID" value="AGZ43127"/>
        <property type="gene ID" value="AFR_24305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4923"/>
    <dbReference type="eggNOG" id="COG2890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076082_0_0_11"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044260">
        <property type="term" value="P:cellular macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043412">
        <property type="term" value="P:macromolecule modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="138"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="325" mass="35079" checksum="393081D9E4E5ACFF" modified="2014-01-22" version="1">MTTIGTAAPAAPAQTTAADEALFRLGQALKDRGYHFTTVTPDTHERVNRRPGNAWARDERDVLGWSRPFQDGVLPAPLRDLLDAGGALERHGDGWRSRVRFSTYDSELFVHSAFPTASADAVFFGPDTYRMADAAVAHLADRATPVRRAVDIGCGTGAGAIAVAKRAPQAEVLGVDINPTALRYARINARLAGTGRVRPCRSNLLNDVDGRFDLIISNPPFMIDLARRTYRDGGGPHGNGLTLAVLDTAEQRLAPGGSLVLFSGTGIADGRDPLREAAEQRLAGTDLTWTYREVDPDVYGEELDTPAYQHAERLAVVVLTATRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W308</accession>
    <name>U5W308_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein-glutamate methylesterase/protein-glutamine glutaminase</fullName>
            <ecNumber evidence="3">3.1.1.61</ecNumber>
            <ecNumber evidence="3">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheB</name>
        <name evidence="8" type="ORF">AFR_25690</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>[protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L-glutamyl-[protein] + methanol</text>
            <dbReference type="Rhea" id="RHEA:23236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="3.1.1.61"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CheB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.61" evidence="3"/>
    <dbReference type="EC" id="3.5.1.44" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364069.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25690"/>
    <dbReference type="EnsemblBacteria" id="AGZ43402">
        <property type="protein sequence ID" value="AGZ43402"/>
        <property type="gene ID" value="AFR_25690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5207"/>
    <dbReference type="eggNOG" id="COG2201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_51_0_11"/>
    <dbReference type="OrthoDB" id="1655418at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018277">
        <property type="term" value="P:protein deamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006482">
        <property type="term" value="P:protein demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16432">
        <property type="entry name" value="CheB_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00099">
        <property type="entry name" value="CheB_chemtxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008248">
        <property type="entry name" value="CheB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035909">
        <property type="entry name" value="CheB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000673">
        <property type="entry name" value="Sig_transdc_resp-reg_Me-estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01339">
        <property type="entry name" value="CheB_methylest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000876">
        <property type="entry name" value="RR_chemtxs_CheB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52738">
        <property type="entry name" value="SSF52738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50122">
        <property type="entry name" value="CHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="3"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="CheB-type methylesterase" evidence="7">
        <location>
            <begin position="160"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3 5">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="361" mass="38065" checksum="20D5E0A546B906B7" modified="2014-01-22" version="1">MIRVLVVEDSATMRHALREALATDPELQVVGEAVDGAQAVELVGRLRPDVVTMDMMLPTMSGLVATEHIMAEFPTPILVVSSADRQELFSTYNALAAGAVDVLEKPRGDDSDAGWGRRLCSCVRLVSRIRVITHPRARLDGRSRSGAEPIVPQAPPRAVPIPADEPTVQVVALGASTGGPGALTDLLRALPVAFTTPVLCVQHIAASEPFAVAFSDWLAGQTGRNVSYGTEGTPLRSLAGRVVLAPPDRHLLVRDGILRLSAAPPRHSCRPSVDVLFESVAAEYGSAAAGCLLTGMGRDGAEGLLQMRARGGHTFAQDEASSVVYGMPREAALLGAAAFIMPPSRIAARLGELSQAARARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W362</accession>
    <name>U5W362_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phenylacetate-CoA oxygenase subunit PaaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">paaB</name>
        <name evidence="1" type="ORF">AFR_20815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362807.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20815"/>
    <dbReference type="EnsemblBacteria" id="AGZ42435">
        <property type="protein sequence ID" value="AGZ42435"/>
        <property type="gene ID" value="AFR_20815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4220"/>
    <dbReference type="eggNOG" id="COG3460">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141459_2_0_11"/>
    <dbReference type="OrthoDB" id="2083684at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009359">
        <property type="entry name" value="PaaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038693">
        <property type="entry name" value="PaaB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06243">
        <property type="entry name" value="PaaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030200">
        <property type="entry name" value="PaaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02157">
        <property type="entry name" value="PA_CoA_Oxy2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="98" mass="11252" checksum="7AA75E2E496E4DEF" modified="2014-01-22" version="1">MSEDIKHEWPLFEVFVRAKRGLNHVHVGSLRAADHQMALHHARDLYTRRNEGVSIWVVRSDDVAASSPDEKEAFFAPSGDKVYRHPTYYSIPDSVPHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W391</accession>
    <name>U5W391_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar transport system, permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_26125</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364235.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26125"/>
    <dbReference type="EnsemblBacteria" id="AGZ43487">
        <property type="protein sequence ID" value="AGZ43487"/>
        <property type="gene ID" value="AFR_26125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5295"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_11"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="88"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="33654" checksum="4D17DCB1E0F6C171" modified="2014-01-22" version="1">MTMAPAAPVRTDEPPSVAPVRRSRFRRHLPAWLFLAPALLLFAVFKFVPMFRAVEMSFHEVRPYLGDRFVGLDNYREVLTNPSFTDAIGHTIVLAVGQTVGSLVLGFALALLLEGSSRGLWFVRTAVFLPTVAAMAVVAELWRILYYPAGDGAINSILGWVGVGPSQFLNDAGSSLWSVMGVGIWRGAPYDMMIFLAGLAGVDRTLYEAAAADGAGPLRRLWHVTIPALRPVLTILLTLAAIRGLRVFTEIFLLTNGGPNGASEVVMTLVYKLGLERNELGIASAGSVLLLLATVVLTVAVQVARRRGEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3H8</accession>
    <name>U5W3H8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_27535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27535"/>
    <dbReference type="EnsemblBacteria" id="AGZ43768">
        <property type="protein sequence ID" value="AGZ43768"/>
        <property type="gene ID" value="AFR_27535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5581"/>
    <dbReference type="eggNOG" id="COG0306">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="403" mass="41230" checksum="5AD61E8F69CCC96E" modified="2014-01-22" version="1">MILALVVITALGFDFTNGFHDTANAMATSIATRALKPKVAVALSGILNLVGAFLSVEVALTVTNAVVKIQNSNGTPKPELLVDGGSALLLIVLAGLVGGIVWNLLTWLLGLPSSSSHALFGGLIGASIAGLGWAGVNWNGDGSKLDGVIGKVILPALMSPVIAGVVAAAGTWLIYRVTVGVAKRFTENGFRWGQIGSASLVSLAHGTNDAQKTMGVITLALIAAGDWTDTKNIPFWVKAACALAIALGTYLGGWRIIRTLGKGLVEIAPPQGLAAESAAAAVILSSSHLGFALSTTHVATGSILGSGVGRPGAQVRWRVAGRMVAAWLITLPAAAIVGAVMWWVGNLLGGGLAGSLAIFVILLAAAVFMWLRSRRTPVDHNNVNDEWEGAPPADERVPAGAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W405</accession>
    <name>U5W405_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative AsnC-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21855</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21855"/>
    <dbReference type="EnsemblBacteria" id="AGZ42641">
        <property type="protein sequence ID" value="AGZ42641"/>
        <property type="gene ID" value="AFR_21855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4430"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="12"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="162" mass="17901" checksum="3DEE548E17A58852" modified="2014-01-22" version="1">MPEPHARYLVAVEEIDRAIVGALTADGRLSYTDLAERVGLSVSAVHQRVRRLEQRGVISGYTAKVSYEALELPLTAFVAIRPFDPSQPDDAPERLAHLSEIDSCYSVAGEDFYLLLVRVAGPADLERLLQEIRTAANVTTRTTVVLSTPYENRPPRIEATEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W488</accession>
    <name>U5W488_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27880"/>
    <dbReference type="EnsemblBacteria" id="AGZ43837">
        <property type="protein sequence ID" value="AGZ43837"/>
        <property type="gene ID" value="AFR_27880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5650"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_771237_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="36505" checksum="C93E6BF1F32ED481" modified="2014-01-22" version="1">MTVALGVRAPSFVHLSRLTDDTGLLEHARHAVARREHGYCTDDVARGLVVTSRERDPAPDVLRLAETYLGFLTHAQHDSGAFHNRLGFDRRWQDEPGLGDWWGRALWGLGTAAARSTAPWIRQEAMVAFTLGAARRSPAPHTMAFAGLGAAEVLRREPGNTLAAALLSDAATAVGWPDSDPQWSWPGRHLSYASAALAEVVIAAGQFQQDVALRAAGLRMLSWLRDLQLDRGHLSLLPASGWRHGTPRQRYDQQPIEAAALADACATAAAATGDTGWNDGVRQAIGWFLGDNDRGTVMWDPETGGGYDGLTPLGANLNQGAESTLALISTFQHAQRING</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W490</accession>
    <name>U5W490_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28440"/>
    <dbReference type="EnsemblBacteria" id="AGZ43949">
        <property type="protein sequence ID" value="AGZ43949"/>
        <property type="gene ID" value="AFR_28440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5766"/>
    <dbReference type="eggNOG" id="ENOG503359U">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135431_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="135" mass="14901" checksum="E694D972C7DAC463" modified="2014-01-22" version="1">MKPGWPALRREISVAGPRTVEEVWDRYVRPRRWPEWSPQIRSVDYAGERLAPGTSGVVRGPGGLPVRFQILAVHDTDPVRTWSWTASAAGVRLALLHTVEATAGGTRTRLTIVGFGPVVLAYLPLARLALSRLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4E6</accession>
    <name>U5W4E6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28130"/>
    <dbReference type="EnsemblBacteria" id="AGZ43887">
        <property type="protein sequence ID" value="AGZ43887"/>
        <property type="gene ID" value="AFR_28130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5702"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074078_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007995">
        <property type="entry name" value="DUF742"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36221">
        <property type="entry name" value="PTHR36221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05331">
        <property type="entry name" value="DUF742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="104" mass="11532" checksum="4EE41486A2C3E2C5" modified="2014-01-22" version="1">MVRPFMLTGGRTRPLHDGLRIETLLHASPAALSAPLRFESRRIVELCQAPMSIADLAVALKVPLGVIRVIVADLVTDGYLRIEEQLGELPIALIERIRDRVRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4R4</accession>
    <name>U5W4R4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363580.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23610"/>
    <dbReference type="EnsemblBacteria" id="AGZ42990">
        <property type="protein sequence ID" value="AGZ42990"/>
        <property type="gene ID" value="AFR_23610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4784"/>
    <dbReference type="HOGENOM" id="CLU_1923036_0_0_11"/>
    <dbReference type="OrthoDB" id="1952072at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021284">
        <property type="entry name" value="DUF2750"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11042">
        <property type="entry name" value="DUF2750"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="131" mass="14760" checksum="3A3F17CA860C8DD9" modified="2014-01-22" version="1">MAADDISRGEAERLRGLDDAARVARTFEVIAGRGELWVWGDEDEILFTEDARRRTLLPIWPHATVARLENEGDVPGEHAIRIAADVFLKEWLPQLEDDRADIAIFPVEENNAAVLTLAEFHSRMTAALRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Z6</accession>
    <name>U5W4Z6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23740"/>
    <dbReference type="EnsemblBacteria" id="AGZ43016">
        <property type="protein sequence ID" value="AGZ43016"/>
        <property type="gene ID" value="AFR_23740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4808"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108054_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase_6" evidence="1">
        <location>
            <begin position="2"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="12810" checksum="5076EF56534BC4CF" modified="2014-01-22" version="1">MLALGATDVERAAAFWGAALGYRLREGVLGGWGTVLSSADGSGISIALQRSESEPEARPRLHLDLHVADAAEQEAEVARLVGLGAQRVDWDSYPDEPDFVVLADTEGNLFCIVDVSLSAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W565</accession>
    <name>U5W565_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Monovalent cation/H+ antiporter subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_30145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560626.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30145"/>
    <dbReference type="EnsemblBacteria" id="AGZ44289">
        <property type="protein sequence ID" value="AGZ44289"/>
        <property type="gene ID" value="AFR_30145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6106"/>
    <dbReference type="eggNOG" id="COG1863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086615_0_1_11"/>
    <dbReference type="OrthoDB" id="1987527at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="PTHR34584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="19665" checksum="AB3D9190BD8C48BB" modified="2014-01-22" version="1">MSTRWQKWRDRLVAAGGLTVIWVLLWGRPTPITIAGGLLVAAVILTVFPLPQVTVAGRINLVGVLRFAGKFLVDLVAASIQIALLAFRFGHVPRSAVIAVRLRAPSDITLTLTAEALSLVPGSLIVEVDREQGVLYVHVLGMRDSSEADGFRQSVLDLEARIVHALGSTADRDRISRKDLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5E5</accession>
    <name>U5W5E5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ribosomal RNA small subunit methyltransferase B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_30545</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560706.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30545"/>
    <dbReference type="EnsemblBacteria" id="AGZ44369">
        <property type="protein sequence ID" value="AGZ44369"/>
        <property type="gene ID" value="AFR_30545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6184"/>
    <dbReference type="eggNOG" id="COG0144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0781">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_0_3_11"/>
    <dbReference type="OrthoDB" id="1064993at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="Fmu/NOL1/Nop2p_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="8">
        <location>
            <begin position="216"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="318"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="506" mass="53930" checksum="A6A9925CB42943AF" modified="2014-01-22" version="1">MSDPKPADQPRGADRRHSADRPHNAGPRRPAGPGQGRGQDRDTRSGRAPRAGRPASDPARQAAYEAIAAVHRDDAYANLVLSQILREMGLHGRDAAFATELTYGTLRTLGTLDLIIADAAGREVSRIDPPARDALRLGAYQLLLTRVPPHAAVNQTVDLVRSVAPGAAGFANAVMRSISETSFDDWLTKLAPDYAENPVGNLAVVHNHPEWIIRSFAESLGGDLEDTARLLIEDNQPATVHLCARPGRADAVDLADEVGGTPGAFSPYAVYLNGGSPSELAAIRQGRAHVQDEGSQLVAAALLAAPIEGQDNRWLDLCAGPGGKAGLLGSIAAQRGAEVTAVEVADHRARLVEQATVGLPVTVLPTDGRTVGRDPDLNEEGFDRVLVDAPCTGLGSLRRRPESRWRRTPADLPPLTRLQRELLVAALRAVRPGGVVAYVTCSPHMVETQVTVTEGARRSGVEVDFVDARPLLPPGMPKLGNGPTVQLWPHRHGTDAMFLAVLRRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5J0</accession>
    <name>U5W5J0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Holliday junction ATP-dependent DNA helicase RuvA</fullName>
            <ecNumber evidence="8">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">ruvA</name>
        <name evidence="10" type="ORF">AFR_31065</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a complex with RuvB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RuvA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560808.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31065"/>
    <dbReference type="EnsemblBacteria" id="AGZ44473">
        <property type="protein sequence ID" value="AGZ44473"/>
        <property type="gene ID" value="AFR_31065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6288"/>
    <dbReference type="eggNOG" id="COG0632">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087936_2_1_11"/>
    <dbReference type="OrthoDB" id="1370858at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009379">
        <property type="term" value="C:Holliday junction helicase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00031">
        <property type="entry name" value="DNA_helic_RuvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013849">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvA_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000085">
        <property type="entry name" value="RuvA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011114">
        <property type="entry name" value="RuvA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036267">
        <property type="entry name" value="RuvA_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07499">
        <property type="entry name" value="RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01330">
        <property type="entry name" value="RuvA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46929">
        <property type="entry name" value="SSF46929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00084">
        <property type="entry name" value="ruvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="HhH1" evidence="9">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="9">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ44473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="20697" checksum="5E8FBD2D9AC735D6" modified="2014-01-22" version="1">MIASVRGVVAAIANDSAVIEVGGVGLQVQCAPGTLAGLKAGVEARLSTSMIVREDSLTLYGFADDDEKHLFELLQTASGVGPRLAQAVLAVHQPEVVRRAISSGDTATLTRVPGIGKKGAERMVLELRDRIGAVSTGTDGVAGALSGGWQDQVRQGVIALGWSQQQADQAVAAVAETIDGEVPAVPVLLRQAIRLLGRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5P6</accession>
    <name>U5W5P6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spermidine/putrescine import ATP-binding protein potA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560856.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31315"/>
    <dbReference type="EnsemblBacteria" id="AGZ44523">
        <property type="protein sequence ID" value="AGZ44523"/>
        <property type="gene ID" value="AFR_31315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6338"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="32666" checksum="DE73EC1FCBC35ACB" modified="2014-01-22" version="1">MTVIDVSRLQKRYGDTVAVQDVSFAVEGGEIFGILGPNGAGKTTTVECIAGLRTPDDGTITVLDRAPHDPELRKLIGVQLQESQLPEKMTVREALELYSVFYPEPADWQRLAGELGLTAKLGTRFGQLSGGQKQRLSIALALIGNPRIAILDELTTGLDPQARRDTWELVESIRDRGVTVLLVTHFMEEAERLCDRVALIDAGRVVALDTPAGLIAGVQAEQRIRFRPSVAFDTALLTALAEVREVRTQGSQLEVIGEGNLLHAVTATLAAHQIIANDLRIEQASLDDAFVALTGRAAGEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W640</accession>
    <name>U5W640_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="4">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="4">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pdxT</name>
        <name evidence="7" type="ORF">AFR_31085</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="4"/>
    <dbReference type="EC" id="3.5.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560812.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31085"/>
    <dbReference type="EnsemblBacteria" id="AGZ44477">
        <property type="protein sequence ID" value="AGZ44477"/>
        <property type="gene ID" value="AFR_31085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6292"/>
    <dbReference type="eggNOG" id="COG0311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069674_2_0_11"/>
    <dbReference type="OrthoDB" id="1628378at2"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PTHR31559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="L-glutamine binding" evidence="4 6">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-glutamine binding" evidence="4 6">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4 5">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4 5">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="22085" checksum="4A178D5FA0C51294" modified="2014-01-22" version="1">MKDPVIGVLALQGDVREHLGALAESDAVARPIRRPEELDAVDALVIPGGESTTMSNLAITFGLLEPIRKRIAGGLPVYGSCAGMIMLAGTVLDGRPDQESFAGIDMTVRRNAFGRQVDSFEADVQIEDIPGDDFHAVFIRAPWVEEVGDDVQVMGRVTSGPAAGRIVAVRQGNLLATAFHPELTGDLRVHRYFVELVRRAADDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W653</accession>
    <name>U5W653_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560996.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32015"/>
    <dbReference type="EnsemblBacteria" id="AGZ44663">
        <property type="protein sequence ID" value="AGZ44663"/>
        <property type="gene ID" value="AFR_32015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6481"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055817_1_0_11"/>
    <dbReference type="OrthoDB" id="1060221at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043917">
        <property type="entry name" value="DUF5753"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19054">
        <property type="entry name" value="DUF5753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="33587" checksum="38EAFDBE64C8104B" modified="2014-01-22" version="1">MEQHLDPTVQRRRLRVELRRARSETGLTQKQVADELGWSPSKLLRIENGQVGISQTDLRALLGHYEVTDPELIRTLTELAQQGRKQQTWSQYRDVLLPDFMAYLSYEGSASLLRQSDPLLVPGLLQTEEYARAINNAYASADTTEHDIERQTEARLLRQGILERDDPPEMFFILDEAVVSRWVGLKPRDAKIMINQLRRLQELNEYPRVSIQIMPFSQGFHFGMQGGFTMLEFPDPEDDELLFLENRAASVSAREGADVIARYKEEFYKLEEAAVGGPTDLGDFLGKIIERMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6M1</accession>
    <name>U5W6M1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Beta-glucosidase</fullName>
            <ecNumber evidence="3 11">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_31960</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3 11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560985.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31960"/>
    <dbReference type="EnsemblBacteria" id="AGZ44652">
        <property type="protein sequence ID" value="AGZ44652"/>
        <property type="gene ID" value="AFR_31960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6469"/>
    <dbReference type="eggNOG" id="COG2723">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001859_1_3_11"/>
    <dbReference type="OrthoDB" id="654705at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102483">
        <property type="term" value="F:scopolin beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017736">
        <property type="entry name" value="Glyco_hydro_1_beta-glucosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="Glyco_hydro_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="PTHR10353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03356">
        <property type="entry name" value="BGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="427"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 10">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ44652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="467" mass="51401" checksum="2F9AD52E5F1F473B" modified="2014-01-22" version="1">MNPTASSASGLLLPTAALPPSFKWGVATSSYQIEGAPAEDGRTPSIWDTFCRVPGAVVGGDNGDVACDHYHRMPQDVALIKSLGVDTYRFSVAWPRVQPGGKGPVNEAGLAFYDRLTDELLSAGVDPWVTLYHWDLPQELEDAGGWPNRDTAYRFADYAMLVFDKLSDRVDNWTTLNEPWCSAWLGYNVGVHAPGRKDFDAAIAAVHHLLLGHGLATQRMKAAATREMKFGITLNMGTADPATDTQLDRDAARRADGMGLRIYLDPLRKGEYPADMVEDLAARNTAFPVQDGDLEIISTPFEVLGVNFYFGQDFSGTDENGNTEDADGHPVVRGVELDRPRTAMNWPITPERFTQLLVRLHRDYPGLPIVITENGAAFDDTADASGYVADDDRTSYLAEHIAAVAAAIEQGADIQGYFAWSLMDNFEWAEGYAKRFGIVHVDYDTQVRTPKQSALWFRDTISRVRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6M6</accession>
    <name>U5W6M6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31985"/>
    <dbReference type="EnsemblBacteria" id="AGZ44657">
        <property type="protein sequence ID" value="AGZ44657"/>
        <property type="gene ID" value="AFR_31985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6474"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="131" mass="14424" checksum="457670FECBE4162B" modified="2014-01-22" version="1">MSLWLFWKRTGEMVRSNVVAEVTAGSGLSEPELTVLVQVNEAGGRLRQNSLAAATGWDRGRLSHLLTRMESRGYVSRERLRNGVEIVMLAPGQDVITAAQPHLAAAVDRHLTGKLTPAQREALREILTHLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Q1</accession>
    <name>U5W6Q1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26670"/>
    <dbReference type="EnsemblBacteria" id="AGZ43596">
        <property type="protein sequence ID" value="AGZ43596"/>
        <property type="gene ID" value="AFR_26670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5405"/>
    <dbReference type="eggNOG" id="COG2345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078469_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="202" mass="21250" checksum="F8E8D1FB6D2436A8" modified="2014-01-22" version="1">MEAIGALREPARRTVYEYVAAQADPVSRNQVAEAVGIGRTLAAFHLDKLTEAGLLEASSAPRTGGPGAGRPAKVYTRSAVEHAVSLPPRDYRLLAEVLAGAVERAGVEPAAYDAARERGRKLSGGELIPALESLGYEPFIDGSRTVRLRNCPFHALAQTHPGLVCGLSQALVEGLADGARTVRLDPGPTGCCVVIDSKNKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6U8</accession>
    <name>U5W6U8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mg-protoporphyrin IX chelatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_26925</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26925"/>
    <dbReference type="EnsemblBacteria" id="AGZ43646">
        <property type="protein sequence ID" value="AGZ43646"/>
        <property type="gene ID" value="AFR_26925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5456"/>
    <dbReference type="eggNOG" id="COG1239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1240">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016684_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="PTHR32039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="6">
        <location>
            <begin position="419"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="232"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="258"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="589" mass="61345" checksum="4BD80B1B77226780" modified="2014-01-22" version="1">MVGTLDIQRALADGVKAYEPGLLAAAHRGALYVDEVNLLPDHLVDLLLDAAAMGRAHVERDGVSVKHAARFLLVGTMNPEEGEPRPQLVDRFGLVVTVSAPRDAKLRAEVVRRRLAYEAGPDAFAARFAADETALAARILAAREAVPSVLLPDTELDRIARVCLAYGVDGMRADIVVARCAVALAAWHGRDRVTADDIRDAARLALPHRRRKDPLDPPGTDEERLEEALDQAGMDDDPDDDPQPPSGGPDDNGPDSGGGPDSNGTPDSSNRPQSSGGPNSSGPDSSGPDSGNGPDGSSSPDSSNASERGDDSRSSDGTPKEGQASGGAAAVQAGAAYRPRALRIAARGEGGHAGRRSPAFARRGRVVGSRIPRGKLAGAPHLPATLRAAIHRGALTAGAQVVQPRDLREAVHVGREANLVLFVVDASGSMAARKRMTVVKTAVLSLLRDAYQRRDRVGMITFRGRTAEQVLPPTSSHEVGVLRLASLRTGGRTPLAAGLRAAAATIAIERRRDPRRRPLLIVVTDGRATSGPDPALVAPALAGVAAVVVDCESGPIRLGLARRLAAALEADVMPLDALAAGNTPIGKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W784</accession>
    <name>U5W784_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33045"/>
    <dbReference type="EnsemblBacteria" id="AGZ44867">
        <property type="protein sequence ID" value="AGZ44867"/>
        <property type="gene ID" value="AFR_33045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6688"/>
    <dbReference type="eggNOG" id="ENOG502ZBUP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092058_0_0_11"/>
    <dbReference type="OrthoDB" id="2012009at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="23421" checksum="3024D80BFA6AF292" modified="2014-01-22" version="1">MWARAAMWVALPVAGAGVGWVVHQLPEWILRIPFLPMRGPFKLAERLPEPESTIGALALGTIAGLVLAFLADRESLTVRMSGTEVTLTRPGTTRTLPRADVTVAYRERDQLVLLGRTGRELAREPFNVSPRRLAGVFGPAWSDQDPYADAYRRWVPNLPDLPAEAEAVFAARQQALDRGDSDDAKDLRAELARLGFVVRDEKKRQHFRATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W794</accession>
    <name>U5W794_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-binding monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28020"/>
    <dbReference type="EnsemblBacteria" id="AGZ43865">
        <property type="protein sequence ID" value="AGZ43865"/>
        <property type="gene ID" value="AFR_28020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5680"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_19_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="2"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="373" mass="39619" checksum="44665FCFD31A5A08" modified="2014-01-22" version="1">MVIGAGIGGLCAAIGLRRRGWEVTVLERRAAPGAEGSGVTLMANGLRGLDALGVGAAVRGEGRAGTPGGARTSDGRWLSRIEGESMSSQLGTTAVGIHRATLHRILLNALPPETVIHDAQVTDVDPGPPPTVVVRGHDVRHPDLVVAADGIGSRTRSRLWPGHPDPVPSGSMAWLGVTTGPWPHTAVTTVTWGRGEEFGMVPLGDGRVYWWASANAPSADGDLHRRFGAWHDPIPALLDATDPASIIRVDLRHLGTPLPSYRKGSVVVLGDAAHAMTPNLGQGANQAIEDAVVLAAMCDPLADLSEALDAYDRTRRPRSQNIARAALRMSRFGQQLTNPLAVATRNAVMRWTPPRTALRTMSHYADWQPPDPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7W7</accession>
    <name>U5W7W7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_35260</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561644.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35260"/>
    <dbReference type="EnsemblBacteria" id="AGZ45308">
        <property type="protein sequence ID" value="AGZ45308"/>
        <property type="gene ID" value="AFR_35260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7134"/>
    <dbReference type="eggNOG" id="COG2265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014689_7_0_11"/>
    <dbReference type="OrthoDB" id="1421660at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="PTHR11061"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRAM" evidence="5">
        <location>
            <begin position="4"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="425" mass="44394" checksum="38909CD4624B40D9" modified="2014-01-22" version="1">MTDELYEGDRVEVTVGAPAHGGHCVARIGGAHGRVVFVRHALPGERVTVELTELHKGYLRGDAVEIHEASPDRVEAPCVYAHPGGCGGCDLQHVAPPAQLAWKTSIVRELLGRMGGLSAERIAELNVEVEPLPGGPLGWRSRVRYAVDAAGRAGLLQHRSHQVVPVDRCLIAHPALQELDVTAKPWPDAAAVEVVASTGGDVSVVARAAGGPLLSPVDGDPLLDEAPGGSRVLVDGPAEVREQAAGREWRVPPEGFWQVHPAAADTLVSTVLELLQPAPGETSWDLYGGAGLFAAAVAGRTGARTTVVESSPVGVAAARTNLADLRGVEVVPAKVDVALTRRRITGPVDLVVLDPPRTGAGAKVVKGITAAGPRAVAYVACDPAALSRDVQTFGAEGYRLAELRAFDCFPMTQHIECVALLVRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7W8</accession>
    <name>U5W7W8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_34195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561434.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34195"/>
    <dbReference type="EnsemblBacteria" id="AGZ45097">
        <property type="protein sequence ID" value="AGZ45097"/>
        <property type="gene ID" value="AFR_34195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6919"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="142"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23358" checksum="5EFDC45347305AAD" modified="2014-01-22" version="1">MTEPTIRLLIVDDHPVVRNGLSSMFAAAPGFEVLGEAADGAEAVELAERLRPDVVLMDLRMPGMDGLTAITELARRGVTARVLVLTTYDTDAHVLPAIEAGATGYLLKDAPREELLRGARAAARGESVLSPSVAAWLMNRVRTPAPQLLSRREMEVLELVAAGNTNREAAGRLFITEATVKSHLLSIYAKLGVRDRAAAVTEAFNQRLLTPQPPTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Y6</accession>
    <name>U5W7Y6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561595.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35015"/>
    <dbReference type="EnsemblBacteria" id="AGZ45259">
        <property type="protein sequence ID" value="AGZ45259"/>
        <property type="gene ID" value="AFR_35015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7085"/>
    <dbReference type="eggNOG" id="COG2220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020884_0_0_11"/>
    <dbReference type="OrthoDB" id="767622at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="127"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="376" mass="41292" checksum="3F938D43879B92FE" modified="2014-01-22" version="1">MAKQANSRRGRTGAWIALALGAAAVWAARDVPLQMGSRKIGAKADGERGERYRRSPQFADGKFVNTVPAVQPAAAAMPRIVAASLTDRDRRHPSAPIPVIRPEPGDASGLHVTWYGHSSALIEIEGRRVLIDPVWSERCSPSRLTGPKRLHEPPIALRELPPIDAVLISHDHYDHLDMDSIRNLVDLQAAPFLVPLGVGAHLERWGVPATRIIELDWNENATVAGIDFTATAARHFSGRGFTRDETLWSSWVIAGPTRKAFYSGDTGYFPGFAQIGEEHGPFDVTLVQIGAYGDAWPDIHMLPEDGVATHVDVQGGLMIPVHWATFNLALHDWPEPADRAWREAKARDVKLAVPRPGERVDVDNPPAVDGWWQQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W865</accession>
    <name>U5W865_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561539.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45202">
        <property type="protein sequence ID" value="AGZ45202"/>
        <property type="gene ID" value="AFR_34730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7027"/>
    <dbReference type="HOGENOM" id="CLU_896117_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666425" evidence="1">
        <location>
            <begin position="28"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="310" mass="32642" checksum="817B0EBAA92468BB" modified="2014-01-22" version="1">MRKLAAAPVLALLMLGACSDPPKTTLPEVTTPVVAAPDLPSGLYARKDDQLRIYDATSGKLTRTVTVPGGPGMRFSADWAYAAEVENGTGRVVLYGLQNDKYVDVGGWSEITLGTPATNVGFVARTGLLAVELNGGDGGAQKTVSVDPRRPAEAPKDLTVTFVWGWDSYGEGASVENVLLSDPPGRVNLHRTSRELLDATIIDQDVDQDGASELLYQCAGPEVAEFTVLCHGEGTGVVAALTAGPADSTAALRRIANLKGREIDAVFRSPDSKSVLAQTETGFYTTSVQGGKLKRAFGPLPGKPEILSWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8A9</accession>
    <name>U5W8A9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RibD_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34930"/>
    <dbReference type="EnsemblBacteria" id="AGZ45242">
        <property type="protein sequence ID" value="AGZ45242"/>
        <property type="gene ID" value="AFR_34930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7068"/>
    <dbReference type="eggNOG" id="COG1985">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036590_7_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="45"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="27289" checksum="4EEBC50F10DFC126" modified="2014-01-22" version="1">MPGRVVVRLPAPAGRAHAARDRTGIAVIDVHDADLVALYPRNPTPTLRVNFITGLDGAATVDGKSAGLGGPGDKLIFDSLRRVCDALVVAAGTVRAEKYDGLRLDAAAREWRRSAGLPEFPLMVVVSGSLELDPAQLVFADAPIRPLVITHAAAPADRRAALQPVADVLTCGDTSIDLAAAVEALHARGATQLLCEGGPHLFGSLIAADLVDELCLTVSPQLTGGSAGRIATGPDTPPRSMSLRSVLADQDMLFLRYARSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8D7</accession>
    <name>U5W8D7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Beta-lactamase class C-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_36085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36085"/>
    <dbReference type="EnsemblBacteria" id="AGZ45473">
        <property type="protein sequence ID" value="AGZ45473"/>
        <property type="gene ID" value="AFR_36085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7303"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_7_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase" evidence="3">
        <location>
            <begin position="41"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="467" mass="48261" checksum="0EE1A8B97E1195D8" modified="2014-01-22" version="1">MAAVLAWLVAPAPPRLDTTGLPAQLTDILGDDTKGYRDLSVTVIEGGTATTASTGESLFEIGSVTKVLTGMLFADLVAEGMVRKDLTLGEAFPAADLKRTTASITLEELASQRSGLPRLAPGPETYLQAMVSGLTGSNPYAGDDVKTLLSAAERAEPGDHRGKVSYSNFGMALLGHALAAHTQKPYAQLVTERILTPLGLSATTIDPAPADRSPGSRATGSQVDPWSSTGYAPAGVGPRSTAADLGKLVAATLAGTAPGADAATPRFTDGDGSRIGYAWFTTRYGTQEITWHNGATGGFTSYVAFDPAAGKGVVVLGNTDRPVERIGLRLLGATPPEESRSVVLPVVTVLLLTWAASALLYLAASARRTVPEAGKAAWWESKPDRLRAVGTVLSTAALLVLLHRIGAWLVIPPATWAVAAGLAVAGLIAVAATWRNLPTHTGGAAWIRWAGLTISAMISTAVIVAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8P7</accession>
    <name>U5W8P7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 8">Protein RecA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7 8">Recombinase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 12" type="primary">recA</name>
        <name evidence="12" type="ORF">AFR_35655</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 9">Belongs to the RecA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561723.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35655"/>
    <dbReference type="EnsemblBacteria" id="AGZ45387">
        <property type="protein sequence ID" value="AGZ45387"/>
        <property type="gene ID" value="AFR_35655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7217"/>
    <dbReference type="eggNOG" id="COG0468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040469_3_2_11"/>
    <dbReference type="OrthoDB" id="1080436at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00983">
        <property type="entry name" value="recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00268">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013765">
        <property type="entry name" value="DNA_recomb/repair_RecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020584">
        <property type="entry name" value="DNA_recomb/repair_RecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023400">
        <property type="entry name" value="RecA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45900">
        <property type="entry name" value="PTHR45900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00154">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00142">
        <property type="entry name" value="RECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54752">
        <property type="entry name" value="SSF54752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02012">
        <property type="entry name" value="tigrfam_recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00321">
        <property type="entry name" value="RECA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="RECA_2" evidence="10">
        <location>
            <begin position="37"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="RECA_3" evidence="11">
        <location>
            <begin position="201"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="67"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ45387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="348" mass="36964" checksum="E822DAE2ABC8F45C" modified="2014-01-22" version="1">MVAGPDRDKALDLALAQIDKQFGKGSVMRLGERPVTQMAVIPTGSIALDIALGVGGLPRGRVIEVYGPESSGKTTVALHAVANAQRNGGIAAFIDAEHALDPDYAQALGVDTDALLVSQPDTGEQALEIADMLIRSGALDIIVIDSVAALVPRAEIEGEMGDSHVGLQARLMSQALRKITGILNNSGTTAIFINQLREKIGVMFGSPETTTGGRALKFYSSVRLDVRRIESLKDGTDVVGNRTRVKVVKNKVAAPFKQAEFDIMYGKGISREGSLIDVGVEQSIVRKSGAWYTYDGDQLGQGKEKAREFLKENPDVAAEIEKKILEKLGVGQTTGDAAGGPELPPVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Q5</accession>
    <name>U5W8Q5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uma2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36365"/>
    <dbReference type="EnsemblBacteria" id="AGZ45529">
        <property type="protein sequence ID" value="AGZ45529"/>
        <property type="gene ID" value="AFR_36365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7359"/>
    <dbReference type="eggNOG" id="COG4636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076312_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06260">
        <property type="entry name" value="DUF820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012296">
        <property type="entry name" value="Nuclease_put_TT1808"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008538">
        <property type="entry name" value="Uma2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05685">
        <property type="entry name" value="Uma2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uma2" evidence="1">
        <location>
            <begin position="27"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="23091" checksum="28F2FD9669585DE8" modified="2014-01-22" version="1">MVTGDPFEEEPMIAALNLADKRDWTVDDVARLPEDLPYELIDGRLALTPAPLPIHQSLGIEIVLALRANWPAGYYLATDQSVLVDHRNEPRPDVVLMRREGANRTPVLAADVILVAEVVSPSSRVSDREDKMKLYSYAGIPAYWIIDPLGDRITFSQFLLSSGGTYHCQLQAEGLVTIDQPWKVTLDLAAWTQERDELREDARPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8S8</accession>
    <name>U5W8S8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561758.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35830"/>
    <dbReference type="EnsemblBacteria" id="AGZ45422">
        <property type="protein sequence ID" value="AGZ45422"/>
        <property type="gene ID" value="AFR_35830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7252"/>
    <dbReference type="eggNOG" id="ENOG5032SH9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124431_1_0_11"/>
    <dbReference type="OrthoDB" id="2010326at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021218">
        <property type="entry name" value="DUF2784"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10861">
        <property type="entry name" value="DUF2784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="14109" checksum="625F84BBAD4CD39F" modified="2014-01-22" version="1">MGYRILIAAAVGAHFAFLAFGIFGGFLAWRWPKLLWLQVLSATWLLVIVVAGLSCPLTWLEDRGREGAGLPALNGGFLDTHVAGVFYPHGYEWVARIVVAVVILTSWAGLVVRSRSRSRSRSELGDRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8U1</accession>
    <name>U5W8U1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="4 7">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">ribH</name>
        <name evidence="8" type="ORF">AFR_30505</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560698.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30505"/>
    <dbReference type="EnsemblBacteria" id="AGZ44361">
        <property type="protein sequence ID" value="AGZ44361"/>
        <property type="gene ID" value="AFR_30505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6176"/>
    <dbReference type="eggNOG" id="COG0054">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_1_2_11"/>
    <dbReference type="OrthoDB" id="1680292at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="1-deoxy-L-glycero-tetrulose 4-phosphate binding" evidence="7">
        <location>
            <begin position="85"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="16274" checksum="6AABCD3620EEDF91" modified="2014-01-22" version="1">MAGFGDPHLGTVDAAGLKLGIVGSRWHADLVDHMVERAQAAAKACGVDDVIVARVAGSVELPIVAQALAKRCDAVVALGVVIRGETAHFDYVNDSVTAGLTRVALDEQTPVAHGVLTVESLGQAKDRAGLEDSIEDKGWQSTVAVLDAVLAIRDLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8W3</accession>
    <name>U5W8W3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561793.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36005"/>
    <dbReference type="EnsemblBacteria" id="AGZ45457">
        <property type="protein sequence ID" value="AGZ45457"/>
        <property type="gene ID" value="AFR_36005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7288"/>
    <dbReference type="eggNOG" id="ENOG5031H1M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1174338_0_0_11"/>
    <dbReference type="OrthoDB" id="1424292at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="25995" checksum="D6EAEC91365B995F" modified="2014-01-22" version="1">MSQDEYDLANPRWYLATRLRDVILRRWSAEVRAIGVHGSLAHGDDSDSSDLNLHVVTYRAQAGPRAALRRVDGILVDLSVGTADDGLRRATELTARWPLEADRYLTTRDLFDPQGWFGSQRDAHLGKLAETRPGEFSGLARRNWCVASAAHARAVRLAEWYETDAALVLLAEARLHAAMVTGLLSRTYFRNRADAVKRTGLAGADMTELGAVLKSQAEDLTARGKPVDGPLSAIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8X2</accession>
    <name>U5W8X2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="7">3-dehydroquinase</shortName>
            <ecNumber evidence="5 7">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Type II DHQase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroQ</name>
        <name evidence="11" type="ORF">AFR_30655</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes a trans-dehydration via an enolate intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 7">Homododecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the type-II 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="5 7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560726.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30655"/>
    <dbReference type="EnsemblBacteria" id="AGZ44391">
        <property type="protein sequence ID" value="AGZ44391"/>
        <property type="gene ID" value="AFR_30655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6207"/>
    <dbReference type="eggNOG" id="COG0757">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090968_2_1_11"/>
    <dbReference type="OrthoDB" id="1872106at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00466">
        <property type="entry name" value="DHQase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.9100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00169">
        <property type="entry name" value="AroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001874">
        <property type="entry name" value="DHquinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018509">
        <property type="entry name" value="DHquinase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036441">
        <property type="entry name" value="DHquinase_II_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272">
        <property type="entry name" value="PTHR21272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01220">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001399">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52304">
        <property type="entry name" value="SSF52304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01088">
        <property type="entry name" value="aroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01029">
        <property type="entry name" value="DEHYDROQUINASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6 7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7 9">
        <location>
            <begin position="100"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7 8">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7 10">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ44391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="15310" checksum="1EA8D31B7E5FD9B8" modified="2014-01-22" version="1">MKTYVLNGPNLGRLGTRQVDVYGLTSYADLVALCEAEGKSLGLDVEVKQTDAEHEMLGWLHAAADEQAPVVLNPGAWSHYSYAVRDACALLRAPLVEVHISNIHAREEFRHHSVVSAVATGVIAGLGVDGYRLALRHIAGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9B1</accession>
    <name>U5W9B1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pirin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560883.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31450"/>
    <dbReference type="EnsemblBacteria" id="AGZ44550">
        <property type="protein sequence ID" value="AGZ44550"/>
        <property type="gene ID" value="AFR_31450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6365"/>
    <dbReference type="eggNOG" id="COG1741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045717_5_1_11"/>
    <dbReference type="OrthoDB" id="833478at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PTHR13903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin" evidence="3">
        <location>
            <begin position="44"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin_C" evidence="4">
        <location>
            <begin position="204"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="34733" checksum="341A8D00F9DE273A" modified="2014-01-22" version="1">MPAITVDDVLVLPRLPQLDPATTSYRPVRRLTTAPTGYEGEGFPVRRAFAGVPTNELDPFIHLDQMGEVDYAPGEPKGTPWHPHRGFETVTYMIDGQMDHQDSNGGGGSITNGDTQWMTAGGGILHIEAPPEQLVTSGGLFHGLQLWVNLPRVAKMIAPKYQDIRGREASLLTTPDGGSLIRVIAGEIAGHAGPGSTHTPINLAHVTLQPGAQLDLPWQPDYNALVYVMAGRGTVGAEARPIQIGQLAAHGAGDTIRVNADRTQDSNIPAMELFILGGKPIREPVAQYGPFVMNTKAELQQAFEDYQKGRLGVIPADRLPHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9C2</accession>
    <name>U5W9C2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBM6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31525</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560898.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31525"/>
    <dbReference type="EnsemblBacteria" id="AGZ44565">
        <property type="protein sequence ID" value="AGZ44565"/>
        <property type="gene ID" value="AFR_31525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6381"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043495_0_0_11"/>
    <dbReference type="OrthoDB" id="811477at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006584">
        <property type="entry name" value="Cellulose-bd_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018535">
        <property type="entry name" value="DUF1996"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03422">
        <property type="entry name" value="CBM_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09362">
        <property type="entry name" value="DUF1996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00606">
        <property type="entry name" value="CBD_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666216" description="CBM6 domain-containing protein" evidence="3">
        <location>
            <begin position="46"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="4">
        <location>
            <begin position="52"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="495" mass="51304" checksum="1293CDE3D8FAB312" modified="2014-01-22" version="1">MRTSPAHVAPEEAAKRRRRTTRFALASAVAVTLGGSGIYIANANAAETVVPGRVQAEAFAAQSGAQTEGTGDADGGKNVGWLAAGDWLRFDNVAITSKDLTARVASDNSAGGSIELHLGSQTGALLATIPVTRTGGWQKWATVKATGASVPTGGQTVFAVMKSAQQNDFVNLNWFTFGAAGTPSAPASSSTPAPVPTTAAPTPTGGTPSGDGWVTMDKAKWEAQLAAFTKMTPKAVPAGNVRVPEFNASCTVSHSKPDDPIVFPGLAGASHMHTFMGNDSTDANTTTMTLLENAGSSCKPGEDRSAYWIPELTENGKKVDPHGVTVYYGSRLKDPAKTVPFPQGFRMIVGDAKKQIDTPKGAPGQFWCAGAGGETGRSTDGNWPVCAKTAELTFQLTFPDCWDGVHLDSPDHKSHIGGTGPDGTCASGKFPVAIPSLSFVIGYPTSGSPAGFKLASGNASSMHGDAFFAWDDEALGSRVKNCIVQKAKCDSFGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA77</accession>
    <name>U5WA77_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32915"/>
    <dbReference type="EnsemblBacteria" id="AGZ44841">
        <property type="protein sequence ID" value="AGZ44841"/>
        <property type="gene ID" value="AFR_32915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6661"/>
    <dbReference type="HOGENOM" id="CLU_113193_0_0_11"/>
    <dbReference type="OrthoDB" id="1798556at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="18791" checksum="F7AFA67294306607" modified="2014-01-22" version="1">MFALAGAGVTLLVSARDNYARSRAKRTKRWYAERKEAYVALMTEFERATYRLRAAYDGDQPAPSPFAYLDAVGPALMQVRLLASGQVRSAALAVHLLIESLYAERPTPPPGVNPEKSVREMLGHVPLVMQQFEAAVREELEIKATPPAPLVDGPRRGWRRKTEESRVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAI4</accession>
    <name>U5WAI4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33710"/>
    <dbReference type="EnsemblBacteria" id="AGZ45000">
        <property type="protein sequence ID" value="AGZ45000"/>
        <property type="gene ID" value="AFR_33710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6823"/>
    <dbReference type="eggNOG" id="ENOG5030NPV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_641961_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="437" mass="44960" checksum="56006DB0EF85E885" modified="2014-01-22" version="1">MSAEQTRRRQPHGGGGHRHAGPVGLRRAGRLRELLVSGVADSFGMALGWTILVLLAVSRGGLAEAALYNAAMLIGVVLSAPVTGWLARRLPGRHLLRGAAGIEIVLRVAALAGLIAGLPSWLIAFLIVAMHVAAWVGFAAMRAEVAAVDARPRAMTRYALAIAAVEAAGTGLGALLPSGPEGHPTGGTLIAIFIVYAGSLIPTIVVARRARMTPQGAAGRIAPARILGYGLSSPVLKRPSAVHRRSRRFPVSPRLLVAGGGIMLLSSGPTLLAVPLTTELHGRHWVAGAAVAFSIGCLLATVAVETIGNMRLPAVLRWSLWGLGMLVGWIGAPLHAANVLAAQFLAGLSQTAFEGDMDAAVADEAPPDGITTALAYSAATRALGGSIAVKTLPVLVTAQAIDKAVSAAVLVLGIASLLLWALTSMPWLNRRPAALAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAK4</accession>
    <name>U5WAK4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Deoxyribose-phosphate aldolase</fullName>
            <ecNumber evidence="2">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_39500</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-deoxy-D-ribose 5-phosphate = acetaldehyde + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562488.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39500"/>
    <dbReference type="EnsemblBacteria" id="AGZ46154">
        <property type="protein sequence ID" value="AGZ46154"/>
        <property type="gene ID" value="AFR_39500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7991"/>
    <dbReference type="eggNOG" id="COG0274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053595_3_0_11"/>
    <dbReference type="OrthoDB" id="1227369at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00959">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="PTHR10889"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00126">
        <property type="entry name" value="deoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="33231" checksum="B0FDF6B1C413841E" modified="2014-01-22" version="1">MTATATSRLPDVSGSAFNLRSFLHGLPGVDKVGVEARAASLGTRSVKTTAKAAAIDLAIRMVDLTTLEGSDTPGKVRALSAKAVRPDPSDPTCPSVAAICVYPAMVPVAAEVLAGTGVHLASVATAFPSGQAPLDVKLADTRDAVAGGADEIDMVISRGAFLSGRYDHVYDEIVAVKEACGSAHLKVILETGELGTYDNVRRASWLAMLAGADFIKTSTGKVPVAATLPVTLVMLEAVRDFRAQTGRQVGVKPAGGIRTTKDAIKYLVLINETVGDDWLDPDWFRFGASSLLNDLLMQRTKLTTGHYAGPDYFTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAW0</accession>
    <name>U5WAW0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FMN_bind domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34015</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561398.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34015"/>
    <dbReference type="EnsemblBacteria" id="AGZ45061">
        <property type="protein sequence ID" value="AGZ45061"/>
        <property type="gene ID" value="AFR_34015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6885"/>
    <dbReference type="eggNOG" id="COG3976">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065810_1_0_11"/>
    <dbReference type="OrthoDB" id="1981023at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007329">
        <property type="entry name" value="FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04205">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00900">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666251" description="FMN_bind domain-containing protein" evidence="2">
        <location>
            <begin position="34"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="FMN_bind" evidence="3">
        <location>
            <begin position="90"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="16998" checksum="0615C99AE8A5EA93" modified="2014-01-22" version="1">MRRSTAAAVGTLTGAALIVGVRLSVTAPVVPSAAPPVVDLANSGQDADPSASPTKKPASKKPKAPAEDAESEESSGEAGGTGLTNGIFKGKAAKNPYGTIQVSIKVSGGKITAADATYPVTGDSATINPPAIASLKESTVQAQSAEVDAVSGATFTSESYVKSLQAAIDAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB76</accession>
    <name>U5WB76_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40725</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40725"/>
    <dbReference type="EnsemblBacteria" id="AGZ46399">
        <property type="protein sequence ID" value="AGZ46399"/>
        <property type="gene ID" value="AFR_40725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8244"/>
    <dbReference type="eggNOG" id="ENOG5032Y6I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154652_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010814">
        <property type="entry name" value="DUF1416"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07210">
        <property type="entry name" value="DUF1416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="111" mass="11366" checksum="9F09C49AC06E46F4" modified="2014-01-22" version="1">MTMTSPSNIADGCAAPDQSAPIPASVDLQKETVITGIVTNAEGEAVGGAYVRLLDSTGEFTAEVVTSPEGVFRFFAAPGSWTLRALSRHGNGDLTVDATRGVNEVGLNVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBB1</accession>
    <name>U5WBB1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561601.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45265">
        <property type="protein sequence ID" value="AGZ45265"/>
        <property type="gene ID" value="AFR_35045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7091"/>
    <dbReference type="HOGENOM" id="CLU_2056296_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="119" mass="12774" checksum="D1D1C6536932041F" modified="2014-01-22" version="1">MPAACRPAGLPAEPPAADTRLSREAVVYVTQESTPEQRNYIDAAIFRVMAAGPGNFYYDPLSPEFRRAYCGRAPLDPKIGPTLPYLYEVGLSSPGAFPALVNEVQGMPGVVGVRHALPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBB5</accession>
    <name>U5WBB5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative microtubule-severing ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_40925</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562771.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40925"/>
    <dbReference type="EnsemblBacteria" id="AGZ46439">
        <property type="protein sequence ID" value="AGZ46439"/>
        <property type="gene ID" value="AFR_40925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8285"/>
    <dbReference type="eggNOG" id="COG1222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_3_11"/>
    <dbReference type="OrthoDB" id="1115436at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48_N" evidence="4">
        <location>
            <begin position="6"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="254"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="507"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="737" mass="77012" checksum="60BCB7E547730F9D" modified="2014-01-22" version="1">MPDDLTLTVTQRTAALDARRGIVRLHPEVMAALAINPGDPLRLAGRRTTAGIAARAESTASRALLYADDLILGNLELRDGGQVTVTPIPVVEARRVTLSGPAEIVAVVSPEMLRLALLGKVVTAGDDVSLLPQDVLPNASHRPLVEAARRSLANRVGYAWTSTLLSVIEVEGADAALVTMDTVVGWQDGQFSSVRDQAAPATAAASQPASTDPETPPPSLDDLPGLKMQAQELTELLDLGFHHGEVLGRLGTTVSLGVLVTGPAGSGKSALVRAVAAGVGATVQSVWGPELAALTNDSAATRLRGLARDVRAGGPAVLLIADVEALAPRDEPGPLATVFRQVLAELLKSGGAVVCTTSRPEAVDPALRAPDLLAVQLAVPLPDAAMRREQLRVLTRGMPLAEDVRLDDVSGRTPGFVAADLGALAREAGVRAALRQKDAESPTVTMADFEAALDVVRPTSMADSTLEVAQVTLDDVGDMVEVKQTLTESVLWPLTYPDTFARLGVQPPRGVLLYGPPGCGKTYLVKAIAGTGKANVLSVKGAELLSKWVGDSERAVRELFRRAREAAPTLVFLDEVDALAPTRGQSTDGGTTDRVVAALLTELDGVESLRNVVVIGATNRPDLIDPALLRPGRLERLVFVPPPDGEARAAILRASAKSVPLDIAVDLDELGASLDGFSAADCAALIREAALSAMRESLDASTVTAAHVDTARKRVRASLDPVQVAWLEAYASTHAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBN8</accession>
    <name>U5WBN8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HxlR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561707.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35575"/>
    <dbReference type="EnsemblBacteria" id="AGZ45371">
        <property type="protein sequence ID" value="AGZ45371"/>
        <property type="gene ID" value="AFR_35575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35575"/>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_5_1_11"/>
    <dbReference type="OrthoDB" id="2057904at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="111" mass="12424" checksum="84AFEEAA570903B0" modified="2014-01-22" version="1">MPKSYSCPVELTVDVVGGRWRTVLLAHLKEGVHRYGELRRLTPGISEKMLSQRLRELEADGLISRKDLGTVPPHVEYDLTDEGRSLTPVLQALYDWGVARAERTGVSIDVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC44</accession>
    <name>U5WC44_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alkaline phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_42645</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563114.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42645"/>
    <dbReference type="EnsemblBacteria" id="AGZ46783">
        <property type="protein sequence ID" value="AGZ46783"/>
        <property type="gene ID" value="AFR_42645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8630"/>
    <dbReference type="eggNOG" id="COG3540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015982_2_1_11"/>
    <dbReference type="OrthoDB" id="165142at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032093">
        <property type="entry name" value="PhoD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16655">
        <property type="entry name" value="PhoD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666508" evidence="2">
        <location>
            <begin position="31"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD_N" evidence="4">
        <location>
            <begin position="44"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD" evidence="3">
        <location>
            <begin position="156"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="549" mass="60753" checksum="7C39757CBF7E30D1" modified="2014-01-22" version="1">MAVSRRTLLLSGAAAGAATAVTTWPLAASAAPYRGPLRSDPFTLGVASGDPDHDGFVLWTRLAPTPLAEDGLGGMPSRDVPVQWELAADEGFRRIVRRGVVAARAAAGHTVHVELNGLLPAREYFYRFKAERYVSPAGRTRTAPPPWATGGTLAMSFVSCSQYEHGYFTAYRRLAEDEPELILHLGDYQYEYAPDTYNIPGGNPRDHEGPETVTLANYRQRHAQYKSDPDLQAAHAVAPWAVVFDDHEIENNWADEVPEQPDPSFLARRAAAFQAYYENMPLRRTSIPRGIDMQLYRRLHWGRLATFHMLDTRQFRDDQGCGDGYKDCPAALDPARSITGAEQEAWLLDGFNRSTARWDILGQQVFFGQRDNNSGPATVVSMDSWDGYAASRDRITRGWVDAGVRNPVVLTGDVHAHWADELKLDYADPTSRTVGTELVCSSITSTGDGADVPTGTHPWTPWNPHLRFYNNQRGYVRTKITKDALTADFQVLPYVTRPGAPVHTRATFVIEDRVPGLHQTADNPTPGGNPALRTGDLGSETVRQETERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCI3</accession>
    <name>U5WCI3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562045.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37280"/>
    <dbReference type="EnsemblBacteria" id="AGZ45710">
        <property type="protein sequence ID" value="AGZ45710"/>
        <property type="gene ID" value="AFR_37280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7542"/>
    <dbReference type="eggNOG" id="ENOG5031TE5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080250_0_0_11"/>
    <dbReference type="OrthoDB" id="1905763at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="25663" checksum="CF7F182E42248163" modified="2014-01-22" version="1">MTTRDRDYQLMMNALADVARRRDEALENAEQAYQTSSAQAAGELSRAENDAISADRWAGAAAAQVLDVDREAARLWDELRRARGMRVKALGELPEPAAVEALPRVALESSPDSGAAAPAPRQSARTLLSRAADRIDVTVRPSQRRSLPRWALPLLPLLGAFVAGLCGLIAAGLVTFGGTGIPGGAVLRGLGWLAFVVAPSAGVPVAAALAHRRLRSRLDIGGIGLTLLGGMLAATVLSVTFASSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCV9</accession>
    <name>U5WCV9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_37955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37955"/>
    <dbReference type="EnsemblBacteria" id="AGZ45845">
        <property type="protein sequence ID" value="AGZ45845"/>
        <property type="gene ID" value="AFR_37955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7676"/>
    <dbReference type="eggNOG" id="COG3176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058962_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="27242" checksum="3164E583F9DEE035" modified="2014-01-22" version="1">MTAAASTGTSGYSLLIADDADQVAAAQRLRHDVFATELGATLRGATPDGRDTDEFDDFCDHLIVRDDTTGDVVGTYRMMTPRAAERAGRRYGDGEFNLSGLRPLRDQLVETGRSCVHPDHRSGAVVNLMWAGLARYLHLNNLRWLGGCASVGLEDGGVTAAGVRARVAAKHLSPPALRVQPRNPWVSDLPGDPRIQPPPLLKGYLRLGSWICGEPAYDPDFECADFYVLFSMDRMDPRYRRHFLGATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE34</accession>
    <name>U5WE34_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40155"/>
    <dbReference type="EnsemblBacteria" id="AGZ46285">
        <property type="protein sequence ID" value="AGZ46285"/>
        <property type="gene ID" value="AFR_40155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8127"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034941_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043426">
        <property type="entry name" value="MltB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30163">
        <property type="entry name" value="PTHR30163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="180" mass="18928" checksum="E4FEFE2B45C39BD4" modified="2014-01-22" version="1">MRAGIPVVAVQAYGYAELVVARTAPSCHLTWTTIAAIAKVESSHGSANGAVLSVDGSVAPPIFGLPLDGKGGRQLIQDTDQGTLDGDAQFDRAMGPLQFIPQTWTSVSTGNSVDADNNGVSDPNDIDDASLAAAKYLCAGGRDLSKPDAWWDAILSYNAVRPYAQKVFDAANEYGQRSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP03</accession>
    <name>U5VP03_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357462.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01150"/>
    <dbReference type="EnsemblBacteria" id="AGZ38519">
        <property type="protein sequence ID" value="AGZ38519"/>
        <property type="gene ID" value="AFR_01150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.229"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_4_11"/>
    <dbReference type="OrthoDB" id="1498437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="20"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="27347" checksum="717CBC3A7224AA4F" modified="2014-01-22" version="1">MQRVKIDHLSCLVTGSGPPLVLLHGLAGSAAEMMAVSLPGRRLIAPDQRGHGHSVRRPDDLSREAYVEDVARVITALADGGPVALAGQSMGAHTAMLTAARHPGLVDRLVMLEGGVGGSVDDYPARLGQWFASWPVPFPTREAAVDFLGPGSLATAWAAELEEHPDGFRPRFDADIMEAAIRPVAETARWTEWQQVQAPTLIVRAAESRNPDEEIARMAELRPGTRVVTIPDSGHDAHLDQPESWAKLLRDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPC4</accession>
    <name>U5VPC4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CbiA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01840"/>
    <dbReference type="EnsemblBacteria" id="AGZ38657">
        <property type="protein sequence ID" value="AGZ38657"/>
        <property type="gene ID" value="AFR_01840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.372"/>
    <dbReference type="eggNOG" id="COG0489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042654_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022521">
        <property type="entry name" value="CHP03815_CpaE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03815">
        <property type="entry name" value="CpaE_hom_Actino"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="113"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="349" mass="36045" checksum="B173607209F706E6" modified="2014-01-22" version="1">MTADPDLLDDLLRLAAAGGTEVDVATDPVAARGRYAAAPLVLIGADQATACLRARLPRRPRTVIVGHTAELERAWEVAESLGAEHVAALPLAEPWLVDRFAERLDAAVPGRILAVIGGRGGAGASILAGALAVTAVRTGHRTLLVDADPMGGGLDLVLGWESVDGLRWPALAETGGRVSPPALLQALPHRGDLVLLSFARDQMLAVPSEAMATTLDAGRRGRDVIIADLPRRLDDAAALALDAADRAILIVPAELRATAAATRIAAIAGLHCPDIAVVVRGPAPGRLKASEVARSIGLPLAGSLRPEAAVCRALENGDAPAADGRGPLAELSKRLIRQLTQDERTGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPG1</accession>
    <name>U5VPG1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative FAD-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02000"/>
    <dbReference type="EnsemblBacteria" id="AGZ38689">
        <property type="protein sequence ID" value="AGZ38689"/>
        <property type="gene ID" value="AFR_02000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.405"/>
    <dbReference type="eggNOG" id="COG0644">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="5"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="378" mass="40644" checksum="21E49F2F326B5E5A" modified="2014-01-22" version="1">MVAEWDVAVVGGGPAGLAAALAAASAGARTIVLERAEHPRYKTCGGGLIGASLAVADPRITVPARDTVSAITFTDRGRRAFTRRSAQHPVLTMVRRDDFDFAWYKAAVTAGAQVRQNAQVRTVDQDADTATVTLVSGETITARVIIGADGSAAITSRHVGVTFEQQDLGLEVELAATDADRAAYRGRVLLDWGPVPGSYGWVFPKDDELTVGVIMAKGKGAATKQYLADFRAQLGLTDRRVVRDSGHLTRCRRPDAPLRKGRILVVGDAAGLLEPWTREGISYALRSGTWAGEVAARAEIPADLAEYDRLITHELMPEMTAGRRLLQVFSRRRALVHAAMATPIGWRAFEAFCRGDLSMANVVRRPAIHALITLLGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPQ5</accession>
    <name>U5VPQ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357735.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02515"/>
    <dbReference type="EnsemblBacteria" id="AGZ38792">
        <property type="protein sequence ID" value="AGZ38792"/>
        <property type="gene ID" value="AFR_02515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.505"/>
    <dbReference type="eggNOG" id="COG0718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124457_0_0_11"/>
    <dbReference type="OrthoDB" id="1715374at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="15095" checksum="413776A9208D61C1" modified="2014-01-22" version="1">MDFNERIEHLFEEYQRQRASFSTLQQQMREISASATSARHEVTVTVGQSGVLTDIRFPTSAYKRLTPAELTATIMQTFAEAKEEATSQAAAILAPMLPDGMDARAMVSGQAGVDTYLPAEPRMATSVREILGLGRAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPR5</accession>
    <name>U5VPR5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ESAT-6-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WXG100 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357737.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02525"/>
    <dbReference type="EnsemblBacteria" id="AGZ38794">
        <property type="protein sequence ID" value="AGZ38794"/>
        <property type="gene ID" value="AFR_02525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.507"/>
    <dbReference type="eggNOG" id="COG4842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151185_3_2_11"/>
    <dbReference type="OrthoDB" id="1930308at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036689">
        <property type="entry name" value="ESAT-6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010310">
        <property type="entry name" value="T7SS_ESAT-6-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06013">
        <property type="entry name" value="WXG100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140453">
        <property type="entry name" value="SSF140453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03930">
        <property type="entry name" value="WXG100_ESAT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="11435" checksum="86965B64DE4496FA" modified="2014-01-22" version="1">MSSYRFDFTQADATLTDMNQINTRIKTALSDMESSVERTLQEWTGAAQTQYYASKAAWNQAANEMTIYLEQARNTLLQISDNYGTTEQRHAQIWNDVRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQK8</accession>
    <name>U5VQK8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Copper-sensing transcriptional repressor csoR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_04075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CsoR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04075"/>
    <dbReference type="EnsemblBacteria" id="AGZ39104">
        <property type="protein sequence ID" value="AGZ39104"/>
        <property type="gene ID" value="AFR_04075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.823"/>
    <dbReference type="eggNOG" id="COG1937">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130332_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003735">
        <property type="entry name" value="Metal_Tscrpt_repr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038390">
        <property type="entry name" value="Metal_Tscrpt_repr_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33677">
        <property type="entry name" value="PTHR33677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02583">
        <property type="entry name" value="Trns_repr_metal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="10690" checksum="C5F35B4AD2BD9CA0" modified="2014-01-22" version="1">MTTDHEHSGPHGYSGDKAALLGRLRRIEGQIRGLQRMVDEDTYCIDVLTQISAAKSALQAVAVGLLEDHLAHCVVDAARAGDPTAKVKEASDAIARLVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQQ4</accession>
    <name>U5VQQ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative sensor-like histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_05020</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05020"/>
    <dbReference type="EnsemblBacteria" id="AGZ39293">
        <property type="protein sequence ID" value="AGZ39293"/>
        <property type="gene ID" value="AFR_05020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1014"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036172_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="3">
        <location>
            <begin position="286"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="386" mass="41399" checksum="3CAFD71F55FF104D" modified="2014-01-22" version="1">MPVTAVRIALVLLLGFNGLGLLLYAVFWAVLPQQLPDGQQQTRRDLALLLPFGAIGLGVILLQGLLFGDNRVAGTAGWLVAIIAVGAGVIWHQSDPTRRGQWAANPQAPWLAAVVAESDRRSFLFRFIGGGVLVATGVIGVLAVYAPAGSLTAVFNGVIFALVGLLGVGVVVAPLLWRTFSQLRSEREGRIREQERAEVAAMIHDQVLHTLALIQRNSTDIKEVQRLARGQERSLRNWLYKPMASPTERFAAALEQAAAEVEDTYAISVEAVVVGDTQCDERVAALVAAAREALVNAARHAGVETVSLYAEVEDDELSVFVRDRGAGFDPAEVEDTRHGVRGSIIGRMQRHGGQAEIRSAPGDGTEVRLTLPASRESVSAGKETAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRK0</accession>
    <name>U5VRK0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GMP synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358978.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05790"/>
    <dbReference type="EnsemblBacteria" id="AGZ39447">
        <property type="protein sequence ID" value="AGZ39447"/>
        <property type="gene ID" value="AFR_05790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1175"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762138_0_0_11"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="200"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="377" mass="40832" checksum="83863BF44EA5C8E6" modified="2014-01-22" version="1">MIVPTVQGEGVRLRAFEQHDVDDLLAGYGDPEVQRFLPPLPVPFTRAQAESYISDVAPSMFAYGGGLYAIADPETDRLLGGVGLDKMVPNRGQTEIGYWVGPWARGRGVATAAVRALTGHAFHTGMARIELLTHRENAPSQRVALATGFQPEGVRRGVLPNRDGGRDDLLAYARLAGDDPGPVERLLPDLPGGELTDGVVCLRPLTPGDLDFYVALHSVEDVVATTVPPIAPSREEMRRRCAWAQSHWLAGDRADLVIIDVATGTPAGEIGLYYQEPRTGQAMIGYSMLPAWRGRGFPTRAAQLVSLWAFAETGIARLIAGALPTNLGSQRVLEKAGFKREAYLRSRLPGHDGRRSDDLQFALVAEDLLREGARLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRK2</accession>
    <name>U5VRK2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00355"/>
    <dbReference type="EnsemblBacteria" id="AGZ38360">
        <property type="protein sequence ID" value="AGZ38360"/>
        <property type="gene ID" value="AFR_00355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.73"/>
    <dbReference type="eggNOG" id="ENOG5032JHC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056144_0_0_11"/>
    <dbReference type="OrthoDB" id="2081103at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="27604" checksum="C37EA1B73CB6196B" modified="2014-01-22" version="1">MHHQVDGLALSTDILDRATRRHQRRTTTIRIGYALGAAGLAGALAAGLSLGGGAAEPPAVVQAAPASMRLANAATASDNISYRIHLKSGSRVGDGAFDPRTASGYFRVPQDDSVTTEIMVNGTRYIGGEPPLGKLPADKGRGEKYGRYGQYPGKHKSLSLFGNGDAVVGAAAPNPAALFKALSQANATTSENPDGTFHFEYATTTGDVALNSDGRIARMTLTTTWRSTAKGRLDTGRFDTTIELSDYGLKVKVKRPADVVPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRP0</accession>
    <name>U5VRP0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SARP family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_06620</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AfsR/DnrI/RedD regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359144.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06620"/>
    <dbReference type="EnsemblBacteria" id="AGZ39613">
        <property type="protein sequence ID" value="AGZ39613"/>
        <property type="gene ID" value="AFR_06620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1351"/>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3899">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_5_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15831">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005158">
        <property type="entry name" value="BTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03704">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01043">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="978"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1013" mass="107845" checksum="5B525F0C4517B96C" modified="2014-01-22" version="1">MVAFGVLGPVRAETALGPVALKGPRHRAVLARLLIARGRVVPVDTLIDDLWEEPPPGAVGAIRTFVADLRRALEPDRPPRQPARVLITAAPGYALQAEDVDAWRFETAVDESADLLATGQAKAALAGLDEALARWRGPAYAEWADRHWARAEIDRLDELRILAVERRAEALLGLGRAADAVSGLQAHAESHPLREDAWHLLITALYRAGRQADALAALRRVRDTLVDELGVDPGPRLRQLETDILGQAAHLNPPAAPRPEPPPLIGRDDELDRLLQAAGQVVSDGRPQLALVSGDAGAGKTTLAEALQHRLGWTVVWGHAPEYDGAPVAWPWPSADQPGGDPATARFRLRQAAVARVTALAAGGPVLLVADDLHRADEATLDLLAALTAEPWPLLVLGTFRATEISPALTAALARFARAEPVRIQLGGLDEAATGALARTVSGREIDAPTARLIHRRSGGNPFFVRELARLHEGDGDALQQVPAGVRDVIRHRLARLPDPAQTVLQQAAVLGQDIDPEVLAALAGPGTPLLDALDAALRAGFLTEDGPGGQPRFTHLLVRDTLYDDLSALRRAHWHARAASALERLRPDDVAALAHHFGLAGGSTAPQAARYARAAAELAERGSRPHDAARLWRQALDSAAAGRQRLAAVMGLGRALAVTGHLHEARQLRDEAITTAEQLDDPELTAEVLAAFVVPATWTRNDDEVLSARIVAAAEHALPAVRGAQRSRLLSTLALELRGDRTGRGDRAAREAEALAREDGDPVLLAFALNARFMHTFRRTGLARERAAIGAELAEVAAKHDLATFEVLGRLILMQAHSALGDLAAADAHAAAADQLADRYDLPVVGVLTTWYAALRLAVTGDPGAARAYREADGRLAAEDMPGMADGLLALALLSVAADGWADGDFGPYEPWVRPLILISRGRPVEAAEALRQAPESPHDLLREARLCLLARAAEHLDDKIILAELREQLSPAAGELAGAGTGAVTLGPVDDHLSGQPGPPNSHRRTHPTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS47</accession>
    <name>U5VS47_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zonadhesin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39808">
        <property type="protein sequence ID" value="AGZ39808"/>
        <property type="gene ID" value="AFR_07595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07595"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1178228_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="114"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="235" mass="24453" checksum="B3C47D64DC62A2C3" modified="2014-01-22" version="1">MTAAGSGAVPEAGAGRSGSSTSSGPADRPSSSESSAAANPAGSSNPPAGPARGSAKGPGTTYGTARSPEAADQVRSTRSGRSPWSDLEGGFEPDERADDLITAYPSARPGPAGAIHGVGVTALVTNLERSVAFYREMLGFYEIDMGSGSAVLASGDMRLVLRTVRDLSSGAGRQIYLNLEVGDVEAVYEELKRKGVKFVHGPRPVNRGDKLELWAASFHDPDDHNIAITQWRAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS74</accession>
    <name>U5VS74_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Group 1 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_01280</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01280"/>
    <dbReference type="EnsemblBacteria" id="AGZ38545">
        <property type="protein sequence ID" value="AGZ38545"/>
        <property type="gene ID" value="AFR_01280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.256"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_299901_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="578"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="621"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="647"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="670"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="694"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="759"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="785"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="821"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="854"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="5">
        <location>
            <begin position="15"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="4">
        <location>
            <begin position="186"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="999" mass="106938" checksum="BAACAFA81728155D" modified="2014-01-22" version="1">MVICNWRDSGHPMGGGAELYCERVAAELHADGVRVTYLTARAKGQSKREDTTFGTVVRGGGTYTVYLFVLLWLLLHRRTIDGVIDSQNGIPFFTPLALRRRTPVALLIHHVHQDQFLVHFPQPAARVGQFLENQASSWVYGKRAMAAVSPSSRAEIRRRLSLRGPVHLAPCGQEMPPPRHRRPAPVPRIVCVGRLVTQKRFDVLLRAMPELNRRLPGVELHLVGDGEARPELERLAAELGPAARVRFHGRVDDATRDALVDSAWVTASASMGEGWGLSIMEAAAAGVPAVAFSVPGLRDTIRPGETGWLVEPTGDLPTALADTLVEALRTLVEARASQEWSARCVAWAGRFTWAATAAHLSAVLTAEKNRLATIRVERRPGSDNAALIRLPSALLAGADLTTLRVTDLVEIDQDQAALLLTGADEQDGRRVLARIGVDASHPGVRVRLARHRDLLGWRTHPPTRGAAGERATGLAAVPPPRKRAETLARLAMVPAAVFVLALTVRLIAISRAYDIFVDEISYADVARNLATGQGLVLNGGPFHLHPPGFFLVLAGFFDLFGMPADTASLVLHARPVSAVAGAIVCAGTTLLLVRVVRLPIAATAGVLLAVDPFLLRFDSRVMLEAPAMAAAVVGLVLLTVRPRGHRTAVAWGVVCGLLLASAILTKEWYAFVTAVPLVLLCFSVDRVQRRMRLTALATTVLGYASYVLVMASLGMLPEWWSEKVGGLARAAGVHQATGFNQPGAESFTSRIAANLSTFGASYGLIVIGGMVTAALVVARNRRPWQFLFTAGGGSVVTALGVGAFAFIGYAVTLGTLEEQMFYPVMVTSVMIIAAGLDMALRAQARNGFTIRRRLTGAVAVAAAAVALIGDGSIWAGIRTTPDDTYRQLLAWAPRGFPADSTVAATEDVAQFVLSGVRLGQWSDLPALTSNNVDYVVVSTALAERGYGKARPDFVAALDRGAAVVFSAHGRTMGELRVYDVRKLVGTATARTVAVKAVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS99</accession>
    <name>U5VS99_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ubiA</name>
        <name evidence="5" type="ORF">AFR_01405</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01405"/>
    <dbReference type="EnsemblBacteria" id="AGZ38570">
        <property type="protein sequence ID" value="AGZ38570"/>
        <property type="gene ID" value="AFR_01405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.283"/>
    <dbReference type="eggNOG" id="COG0382">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034879_5_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006371">
        <property type="entry name" value="HB_polyprenyl-transferase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PTHR11048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01475">
        <property type="entry name" value="ubiA_other"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="32248" checksum="48570C22AA132A6A" modified="2014-01-22" version="1">MTTVASAPEQPGKVKAFLRLVAIEHSVFALPFAYLSALVAMDRLGTGVHWVDLLLVTVAMVSGRTFAMAANRILDRRIDALNPRTKNRELVTGAVSVRTAWTGAAVSLVVLFVAAGLLNWLCLVLSPLAVIPLIVYPYAKRFTNFPHYVLALAQAVAPVGAWLAITGTFDGSGPAWVLGVAVGLWIGGFDIIYACQDVDVDRQIGVHSTPARFGVRTALRISTVTHVVTFVLFVWFGQLVGLGWLWWVGLALTAVAFAYQHIVVTENDLSKVNRAFFTANGFVGIALFLFALLDLILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSG1</accession>
    <name>U5VSG1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359283.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07315"/>
    <dbReference type="EnsemblBacteria" id="AGZ39752">
        <property type="protein sequence ID" value="AGZ39752"/>
        <property type="gene ID" value="AFR_07315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1493"/>
    <dbReference type="eggNOG" id="COG0561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_0_0_11"/>
    <dbReference type="OrthoDB" id="1861282at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="27363" checksum="0982417D4153F3B5" modified="2014-01-22" version="1">MTLDAVVTDLDGTIIRADGTITEATLSACRTLQARGIPVIAATARTPAGIRALTTLTPHLTLAVCCGGSVGYAPAGETVLWQDVVHADAISGLLAYVRESLPGAGVAAYDGSSWRMTEEYLAIRTSPHNGPTAVVPMEALADVAACGMTLCHPVLRPEEIIAELEQRGLALSLSYAGPELVEIAAAGIDKASGVARALREVRVPGERAVAFGDMPIDIPMFTVVGTSVAMADSPPAVLSAATMVTATVEEDGFTTALTDLGLLPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VST5</accession>
    <name>U5VST5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative ROK-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359460.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08200"/>
    <dbReference type="EnsemblBacteria" id="AGZ39929">
        <property type="protein sequence ID" value="AGZ39929"/>
        <property type="gene ID" value="AFR_08200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1673"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_3_11"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="392" mass="40937" checksum="D6488A14E9DF209A" modified="2014-01-22" version="1">MQVVDPPHLRLLRLLRDEGPISRAELGDRLDLTRPRLLAEVERLVAAGYIAEAGMAASRGGRRSTLVELQPRLRFAAVDLGASSIDIEVTNGRLEPVANYREAADIRSGPKAILHRVNELLAKARTDGAFEKLDAVGIGVPGPVSFRDGVPVSPPIMPGWDRYPVRELLAREHGCPAVVDNDVNIMAIGERHGGVAHSVDDFLFVKIGTGIGCGIHLAGYVYRGVDGCAGDIGHIQVDAHGPMCSCGNAGCLEALFSGAALARDAQAAARSGESPALAERLTLNGELGARDVAEGAAEGDVTCIRLIRDGGRRVGATLATLVSFANPSMIVIGGGLAQLGHILLAEIRSVVYRRSLPLATGNLPVVLSELGARAGVSGAAVLASDTAFEQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSV7</accession>
    <name>U5VSV7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08325"/>
    <dbReference type="EnsemblBacteria" id="AGZ39954">
        <property type="protein sequence ID" value="AGZ39954"/>
        <property type="gene ID" value="AFR_08325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1694"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018334">
        <property type="entry name" value="ArsR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00846">
        <property type="entry name" value="HTH_ARSR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="110" mass="11982" checksum="F8140B13290F6BE8" modified="2014-01-22" version="1">MVACCPPMATSRIPAESAVPLSQAFKALGDPIRLQLMSMIASAEGGEICVCDLTPAFEISGPTISHHLKVLREAGLVDAERRASWVYYRPRPEMMRRLATLLAIDETVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTG0</accession>
    <name>U5VTG0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09945"/>
    <dbReference type="EnsemblBacteria" id="AGZ40278">
        <property type="protein sequence ID" value="AGZ40278"/>
        <property type="gene ID" value="AFR_09945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2028"/>
    <dbReference type="eggNOG" id="COG0398">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2393224_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="93" mass="9591" checksum="D3DEF24D040406F3" modified="2014-01-22" version="1">MLLVRFLPLAPFGLIGYAYGTTSVCRKRYLLGTTLASVPSAVSYAVIGAAVTSPGSMNPLTLAPAAIGIGLTTTIVLRWRLAARRNAAALATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTH5</accession>
    <name>U5VTH5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359744.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09300"/>
    <dbReference type="EnsemblBacteria" id="AGZ40149">
        <property type="protein sequence ID" value="AGZ40149"/>
        <property type="gene ID" value="AFR_09300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1894"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_11"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="PTHR43150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="2">
        <location>
            <begin position="15"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="332" mass="36144" checksum="12D6D2135A08C5A1" modified="2014-01-22" version="1">MEFRHLGRSGLLVSEIAYGNWITHGSQVEEEAADACVKAALDVGITTFDTADAYAGTRAEEVLGRALKGERREGLEIFTKVFWPTGPGPNDRSLSRKHLMESINGSLRRLQLDYVDLYQAHRYDYSTPLEETMQAFADIVHSGKALYIGVSEWSATEIRAGWELAQDLKIQLVSNQPQYSALWRVIEAEVVPTSIELGIGQVVWSPIAQGVLTGKYEPGKQPPAGSRATDDKSGADFISRLLSDEVLTAVQQLKPLAEQAGLSMAQLAVAWVLQNSNVSAAIIGASRPEQVRDNAKASGVKLDDDLMAAIDKALFPVAERDPAKTVSPAQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTJ1</accession>
    <name>U5VTJ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10120"/>
    <dbReference type="EnsemblBacteria" id="AGZ40313">
        <property type="protein sequence ID" value="AGZ40313"/>
        <property type="gene ID" value="AFR_10120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2061"/>
    <dbReference type="HOGENOM" id="CLU_211557_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="55" mass="5354" checksum="420496BC6289172F" modified="2014-01-22" version="1">MSLVPVLLLGLAGILVGGVISLARQGATKFSIGLVAALALLALAGGVVWLIPGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU81</accession>
    <name>U5VU81_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpsG</name>
        <name evidence="10" type="ORF">AFR_04485</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts proteins S9 and S11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358162.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04485"/>
    <dbReference type="EnsemblBacteria" id="AGZ39186">
        <property type="protein sequence ID" value="AGZ39186"/>
        <property type="gene ID" value="AFR_04485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.906"/>
    <dbReference type="eggNOG" id="COG0049">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072226_1_1_11"/>
    <dbReference type="OrthoDB" id="1540940at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_B">
        <property type="entry name" value="Ribosomal_S7_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005717">
        <property type="entry name" value="Ribosomal_S7_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01029">
        <property type="entry name" value="rpsG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S7" evidence="9">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="17593" checksum="24A00DA4FE44A03C" modified="2014-01-22" version="1">MPRKGPAPRHPVVPDPVYNSPLVTQLVNKILVGGKRQLAERIVYGALEGAREKSGTDPVVTLKRAMDNVKPTLEVRSRRVGGATYQVPVEVRTPRQTTLGLRWLVQYSKARREKTMIERLQNELLDASNGLGAAVKRREDTHKMAESNKAFAHYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU90</accession>
    <name>U5VU90_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="9">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">guaA</name>
        <name evidence="12" type="ORF">AFR_04980</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358349.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04980"/>
    <dbReference type="EnsemblBacteria" id="AGZ39285">
        <property type="protein sequence ID" value="AGZ39285"/>
        <property type="gene ID" value="AFR_04980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1006"/>
    <dbReference type="eggNOG" id="COG0518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0519">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_5_11"/>
    <dbReference type="OrthoDB" id="504464at2"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 9 10" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="3 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="11">
        <location>
            <begin position="198"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="225"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ39285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="517" mass="55658" checksum="514A62A7E6B75236" modified="2014-01-22" version="1">MSTPRPVLVVDFGAQYAQLIARRVREARVYSEIVPHSMPVAEMLAKNPAAIILSGGPSSVYETGAPSLDAALFEHDVPVFGICYGFQAMAQALGGTVAHTGAREYGRTLLSPLSEAGTLMRELPADLPVWMSHGDSVTEAPTGYTVTASSPGAPVAAFESLATRRAGVQFHPEVAHTQQGQEMLQRFLYDIAGLEPTWTSANIIDDQVAAIREQVGDKQVICGLSGGVDSAVAAALVHKAIGDQLTCVFVDHGLLRAGEAEQVEKDYVAATGIRLVVVDAEETFLGHLAGVTDPEQKRKIIGREFIRTFEGAARDLDAERHIEFLVQGTLYPDVVESGGGTGTANIKSHHNVGGLPDDLQFALIEPLRTLFKDEVRALGAELGLPEEIVQRHPFPGPGLAIRIIGAVDRERLDVLRAADLIAREELTASGLDREVWQFPVVLLADVRSVGVQGDGRTYGHPVVLRPVSSEDAMTADWSRLPYDVVAKISNRITNEVPEVNRVVLDVTSKPPGTIEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUF0</accession>
    <name>U5VUF0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enoyl-CoA hydratase/isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10790"/>
    <dbReference type="EnsemblBacteria" id="AGZ40447">
        <property type="protein sequence ID" value="AGZ40447"/>
        <property type="gene ID" value="AFR_10790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2199"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1933492_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="130" mass="13581" checksum="F44BCE9637E1C910" modified="2014-01-22" version="1">MALNCDFRVLADDARFSMAEVTLGLVPDLGGTKRLTELVGASRALEICVTGRRISADEADRIGLATAVVPGADLQGAVADLAAAVLAADAGAVSEIKALLAGAPQRSYAEQDRAEREAQTRRLRDLMGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVA1</accession>
    <name>U5VVA1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360796.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12255"/>
    <dbReference type="EnsemblBacteria" id="AGZ40737">
        <property type="protein sequence ID" value="AGZ40737"/>
        <property type="gene ID" value="AFR_12255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2491"/>
    <dbReference type="eggNOG" id="COG2388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132888_0_0_11"/>
    <dbReference type="OrthoDB" id="1872172at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045057">
        <property type="entry name" value="Gcn5-rel_NAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031165">
        <property type="entry name" value="GNAT_YJDJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31435">
        <property type="entry name" value="PTHR31435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14542">
        <property type="entry name" value="Acetyltransf_CG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51729">
        <property type="entry name" value="GNAT_YJDJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="8"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="108" mass="12190" checksum="DB75B98AC0D6B5D3" modified="2014-01-22" version="1">MSDNVDVRDNPAQHRYEIAVDGDLAGFAVYYLEKERIVFIHTEVAIAYEGRGMGSRLARQALDDVRGKGLRVVPACPFIARFIRTHPEYQDLTRGDQSSRAVLGEHDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVK9</accession>
    <name>U5VVK9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sigma-70 family RNA polymerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_12780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12780"/>
    <dbReference type="EnsemblBacteria" id="AGZ40842">
        <property type="protein sequence ID" value="AGZ40842"/>
        <property type="gene ID" value="AFR_12780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2596"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r4_2" evidence="5">
        <location>
            <begin position="121"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="177" mass="19390" checksum="5D9B33375F2F2CB5" modified="2014-01-22" version="1">MRRIAKGDTDAFEQLYRRTSPWLAMRLRLRCSDDGLVAETLQDTYLTVWRAAGSFVDFSSRASGDTPGGATAGWLWTIASRRLVDALRRQGRRRETPAEVMPTISVPAAEDDALAGTVGDELGVALAELAPELRAVLRAMVLDGFTVRETSVLLGVPEGTVKSRARRARTLMREALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW31</accession>
    <name>U5VW31_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39940">
        <property type="protein sequence ID" value="AGZ39940"/>
        <property type="gene ID" value="AFR_08255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08255"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="24521" checksum="600903CB8E7B618A" modified="2014-01-22" version="1">MWLADRFSIPAATRAILFDMDGVLIDSLSQDCEVVGELLAPFCGSHDQVPRTLIRRYFALALDDFWRVLGGELNLVLTDEDVELLVAKHELVRRQAKPIVHDGVLEILDAARRADLACAVVSNNPRSEIENMLEAAELLRYVTQVTGNDELGQRRKPAPDPYVSAANGLGVATSDCVAVEDSIIGLESAHSAGCYTIGVGTGANTVEELAASGFATQCYNDFTVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW35</accession>
    <name>U5VW35_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359402.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07910"/>
    <dbReference type="EnsemblBacteria" id="AGZ39871">
        <property type="protein sequence ID" value="AGZ39871"/>
        <property type="gene ID" value="AFR_07910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1615"/>
    <dbReference type="eggNOG" id="COG0223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033347_2_0_11"/>
    <dbReference type="OrthoDB" id="2009156at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016742">
        <property type="term" value="F:hydroxymethyl-, formyl- and related transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="1">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl_trans_C" evidence="2">
        <location>
            <begin position="204"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="35291" checksum="1413C166F6ABAA4A" modified="2014-01-22" version="1">MRVVMFGFQTWGHRTLQALLDSEHDVVMVVTHPAGEGAYEKVWSDSVAELAESHGVPVLLRERPDDEELYEALKQVAPDVIVATNWRTWIPPRIFTLPVFGTLNVHDALLPAYAGFSPLIWALLNNEPEVGVTAHMMDDTLDAGPIVLQRAVPVGPADTTTDLFDKTLELFGPITVDGLAEIASGRRDFVPQDRSKASFFHKRSEEDIRIDWTWTAEELERLVRAQSAPYPQAFCFHKGRRLEIITAAVSEAVYGGTPGRIFYREGDGVAIVAGAEARRGRSKALLIRSVRTDEGVELPAHDYFRTMGGYLTSHPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW49</accession>
    <name>U5VW49_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative major facilitator superfamily transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_13840</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13840"/>
    <dbReference type="EnsemblBacteria" id="AGZ41054">
        <property type="protein sequence ID" value="AGZ41054"/>
        <property type="gene ID" value="AFR_13840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2810"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038683_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665776" evidence="6">
        <location>
            <begin position="17"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="403" mass="41162" checksum="F02AB58127E09F91" modified="2014-01-22" version="1">MFSRPVSFMVAGAAGAATLTAASAPSPLYPVYQRLWGFSAFTLTVIFAVYVFALLAALLTVGSLSDRTGRRPVASAALVLLALGMLLFAIAGGTGGLIAARVVQGFAVGAATGTVTAMIMDSAPRPQLGSLVSSAVPALGIAIGAVLAGALVEFAPLPRQLVFWILAAVYLVLAALVWLIPEGSRPERAAHSSIWRSLLPSAGLPPAARPTFFALLPSIGATWALAGLYLSLGSSVIGTVLEVHSHFVVGIVLGVFFAAGIAGTAVSTVLPPELREWFGYGTLILGVLITIAAMLTSALPLYVVGSVVAGLGFGAAFRFAVNALGEAAPAEQRGQVFATMYIASYIAFSVPALAAGLAVQRVGLEPTAVTYGAAEVALVLLAMTAGLIQARRRTAEDNTPRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWA2</accession>
    <name>U5VWA2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Class I/II aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_08260</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08260"/>
    <dbReference type="EnsemblBacteria" id="AGZ39941">
        <property type="protein sequence ID" value="AGZ39941"/>
        <property type="gene ID" value="AFR_08260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1683"/>
    <dbReference type="eggNOG" id="COG0079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="3">
        <location>
            <begin position="5"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="25916" checksum="2048DF334C4AB4CA" modified="2014-01-22" version="1">MRGVPYNDDLTFPYERFERALERTPKLIVLINPNNPTGTHIDLAFIEQVVRTHPDVPVIVDEAYFEFTGRTVAPLTAKYDNLLVIRTFSKAFAMAGMRLGYVVAHPGVITQLAKMRNPFDVNSLAMVAAKAQLAHVDEIRAYVDEVMLAIKPSAVNFFSERGVAFWPGAANFVLVRPSNCQAVVVELKAAGILVRPITATPLAGTFRMSMGTQEEMTRVFDVLDGLSFLRKAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWA9</accession>
    <name>U5VWA9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_14260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361193.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41134">
        <property type="protein sequence ID" value="AGZ41134"/>
        <property type="gene ID" value="AFR_14260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2893"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2248925_0_0_11"/>
    <dbReference type="OrthoDB" id="1986546at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="14142" checksum="429EE4A8C24EAF4C" modified="2014-01-22" version="1">MISRQAEFDAPAEVVFGVLTDPDRTTRWLPLGMSSETTTVDQVRVRAGSRIHEYAVSSVPDQLRLEWRSQDSTGPRGTAWVEDSPAGGCVVHAEVSLPPDSAQSERAEQLLAESLHHLQRDVSDNFNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWF6</accession>
    <name>U5VWF6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HxlR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15280"/>
    <dbReference type="EnsemblBacteria" id="AGZ41338">
        <property type="protein sequence ID" value="AGZ41338"/>
        <property type="gene ID" value="AFR_15280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3103"/>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="43"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16602" checksum="3B310855AAFB851E" modified="2014-01-22" version="1">MSPWVVTIGYLLKGGDHAMAGQDSTSVSPGEPGVTGDLFSPACPTRQLLDRIGDKWTSMLVKVLAEAHPGELGFAELRRRTPGVSHKMLSQTLQSLRHDGLVERRVDPVVPPRVYYSLTDLGLSLDAPLAVVRAWAEQHIRAIEEARRRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX38</accession>
    <name>U5VX38_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spermidine/putrescine import ATP-binding protein potA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_15375</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361416.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15375"/>
    <dbReference type="EnsemblBacteria" id="AGZ41357">
        <property type="protein sequence ID" value="AGZ41357"/>
        <property type="gene ID" value="AFR_15375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3122"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="31627" checksum="590A5420EF97AB08" modified="2014-01-22" version="1">MIELIGLTKSYGRVRAVDDVTFSAVPGRVTGFLGLNGSGKTTTLRMLLGLTRPTSGVALIDKVRFQKLSHPARQVGAVLEQGIAHPGQSGRAHLTTQAILAGTGRDRVDDLLEQVGLGDAAEQRCGEYSLGMRQRLAVATALLGDPSVLIMDEPANGLDPEGIAWLRELLREHAHGGGTVLISSHLLAELAQLIDDVVIIADGSIRYAAPLDELYGSTSARLRVRGRDPETLLRVYEEAGATVGTDGEGLEVVGLTPEDAGEIAFEAGVPLYELAVDAPNLEQIFLDLVSTAPAAEPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX61</accession>
    <name>U5VX61_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361441.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41382">
        <property type="protein sequence ID" value="AGZ41382"/>
        <property type="gene ID" value="AFR_15500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3147"/>
    <dbReference type="HOGENOM" id="CLU_1987851_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025459">
        <property type="entry name" value="DUF4279"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14106">
        <property type="entry name" value="DUF4279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13396" checksum="3E73C6AF06CF92EF" modified="2014-01-22" version="1">MASPNRLTFMVRSDLLTADEISVRLGLKPTSVQRARGSRLHIWELASPLAEEASPDDHLNGLLDVIEPKAAGVRKLLDAGAEVFWSCFVTAAPLGAIVELEPPLLSRLAGLGLPLVLDIYDSDPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXJ3</accession>
    <name>U5VXJ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_10485</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TerC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360211.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40386">
        <property type="protein sequence ID" value="AGZ40386"/>
        <property type="gene ID" value="AFR_10485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2137"/>
    <dbReference type="eggNOG" id="COG0861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045644_0_1_11"/>
    <dbReference type="OrthoDB" id="1917125at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005496">
        <property type="entry name" value="Integral_membrane_TerC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022369">
        <property type="entry name" value="Integral_membrane_TerC_rswitch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30238:SF0">
        <property type="entry name" value="PTHR30238:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03741">
        <property type="entry name" value="TerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03718">
        <property type="entry name" value="R_switched_Alx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="36340" checksum="2CC77352AAA99BA4" modified="2014-01-22" version="1">MNVSAWVWIATLIALVVVLAVDLLIVGRRPHEPSMREAGGWVAFYVGLALAFGVGVWVLAGGQAAGEFYTGWLTEYSLSVDNLFVFVIIMARFAVPRQFQQKVLLIGIVLALIMRGAFIAAGAALISEFSWVFYIFGAFLVYTAVTLLKQGENDEDDFKENILIRWSKRALPISSSFDSGKMTMVSETGKRLFTPMLIVMIAIGTTDLIFALDSIPAIFGITKEPYLVFTANVFALMGLRQLYFLLGGLLERLVYLNIGLAFVLAFIGVKLFLEALHTNALSFINGGHGVHWAPEIPIWLSLLVILGTLGIATVASLVKSSRDRKKELIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY29</accession>
    <name>U5VY29_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative diacylglycerol kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361849.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17045"/>
    <dbReference type="EnsemblBacteria" id="AGZ41687">
        <property type="protein sequence ID" value="AGZ41687"/>
        <property type="gene ID" value="AFR_17045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3458"/>
    <dbReference type="eggNOG" id="COG1597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045532_5_1_11"/>
    <dbReference type="OrthoDB" id="869726at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="1">
        <location>
            <begin position="12"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="32894" checksum="B9ECA3B1CEAC2BD9" modified="2014-01-22" version="1">MRTKQQLTDDIRNERRAVLVVNAHARRGRKLYDTALAGLTAAGFTLLGAYPVDRPGELERTLERGIGLGPDLLVVGGGDGTISTGARVLAHRDIAMGLLPLGTTNNFARTVNVPLDLGDAVATLTGGKVVDVDLGLAGDMLFTNHVGVGLSAEVMRKAPRSLKRLTGRLAYPATALALLTRHRPLRATVRAGGREHEFLTHQLYVANGGFHAGRPITADADADDRLLVAYPVGGPTRRTLLRETARNAASGDRRTLGDDPFIAVGELLLETDRPALVEVDGEPCGSTPMRIGLDANALRVMAPLDTVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYT0</accession>
    <name>U5VYT0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative branched-chain amino acid ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18320"/>
    <dbReference type="EnsemblBacteria" id="AGZ41942">
        <property type="protein sequence ID" value="AGZ41942"/>
        <property type="gene ID" value="AFR_18320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3718"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="35152" checksum="9B7CE3E0DB79FC1F" modified="2014-01-22" version="1">MLLAAAVLSFPSLAPNPYILSAGIVVLNYAVLSTSWNFVGGFTGYISLGHGALAGLGGYGTALLVTKAGLPSFVALAVAALLVALLAVPIGLAALRVRGASFVIVSIALVLILLLVFQSWASFTGGSRGLVVPRPFPGLLRPEHHRVFFYLFAALLALALLVWWVIDRSRFGLGLKAVREDEDKAEALGTPTFAYKLIVFVISAGFTALAGGLYALWFGDLDPVFQFSILTGSYLVLMALLGGVRNLFGPLAGALIVGVALEYFKGQFGNTPLHLVATGLLLGLVVMFMPDGVLPAIGSLFRRFGKSEQSSIREVTAAELQEERKREEVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZH4</accession>
    <name>U5VZH4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GMP synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362544.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19500"/>
    <dbReference type="EnsemblBacteria" id="AGZ42172">
        <property type="protein sequence ID" value="AGZ42172"/>
        <property type="gene ID" value="AFR_19500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3957"/>
    <dbReference type="eggNOG" id="COG1606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061181_2_0_11"/>
    <dbReference type="OrthoDB" id="846643at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01990">
        <property type="entry name" value="Alpha_ANH_like_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005232">
        <property type="entry name" value="LarE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF2">
        <property type="entry name" value="PTHR43169:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006661">
        <property type="entry name" value="PP-lp_UCP006661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00268">
        <property type="entry name" value="TIGR00268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine synthetase" evidence="2">
        <location>
            <begin position="12"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile and sulfur donor" evidence="1">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR006661-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28634" checksum="3974FD3D895D3E3B" modified="2014-01-22" version="1">MVSMDFFPGVGRLGVAFSGGVDSSVLLALAVRDLGRDRVVAVLGVSPSLAADEREAAHAVARFIGVAVVEVTTFEGDRDAYRANGPDRCFHCKDELFTRIADEVVTAYRLDAVAYGENADDAQRPDRPGSRAATQHRVLRPLADAGLDKAAVRRLAREFALPCADKPAAPCLASRIPHFQPVSREKLAQVERAEAALRGLGFQDLRVRHHGDVARIELPPADLVRAVADPLRAEVHKAMLAAGFRFAAVDVAGVQSGAFTLPLVQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZI0</accession>
    <name>U5VZI0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361174.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14165"/>
    <dbReference type="EnsemblBacteria" id="AGZ41115">
        <property type="protein sequence ID" value="AGZ41115"/>
        <property type="gene ID" value="AFR_14165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2874"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_34_3_11"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="143" mass="15573" checksum="F0E15692E12CAAB0" modified="2014-01-22" version="1">MSVDIEVVQDITEDLVEAFGRLLPQLSRSAPPLDAAALRALVEWQGNRLLIARIEGEIVGTLTLVTFPIPTGLRAWIEDVVVDEAARGHGVGAALTQAAVRLARADGARTVDLTSRPAREAANRLYEKLGFQVRDSKVYRLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZI9</accession>
    <name>U5VZI9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361184.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14215"/>
    <dbReference type="EnsemblBacteria" id="AGZ41125">
        <property type="protein sequence ID" value="AGZ41125"/>
        <property type="gene ID" value="AFR_14215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2884"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062374_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665918" evidence="1">
        <location>
            <begin position="30"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="389" mass="40204" checksum="2109EC8E0BCC95A5" modified="2014-01-22" version="1">MNRRCLAAVVIAAIGATAGQIPAVGPAAAAAPDAWGQPEANATGDHYNPGETRLTPAVAPKLKPRWKVPLADATCATAGEPLVGGNRLVTAASYRISGYDASTGKRVWQTPATGKKTDISLTAIAGTRLVAQSRDCRSGKTFLSAQAVKTGKVLYSKRIPETMYGTLVDKGIVVGGVWDATISKYAIRAYRITDGTPVWARVGSIGGETVAAGGRILVVGEDKTTAVDVTTGKTLWTAGAGCFTPIGASPNGSTFYMRCGPDEQIRSVDAATGATLETFPTHGGTFGFATDGERVYLHTYDNELIAVDAVDGHKVWTATFAENAPIITSIGGGVVYGWRGKDHPLTAFETATGKAIQLAATTSALRDAPLVANGRLYGATGAAVTAYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZR0</accession>
    <name>U5VZR0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amino_oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21030"/>
    <dbReference type="EnsemblBacteria" id="AGZ42478">
        <property type="protein sequence ID" value="AGZ42478"/>
        <property type="gene ID" value="AFR_21030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4263"/>
    <dbReference type="eggNOG" id="COG3380">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036034_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="1">
        <location>
            <begin position="111"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="31508" checksum="E8AE843915FA21ED" modified="2014-01-22" version="1">MNVVVVGAGIAGLACARELTAAGVDVRVHERGRVVGGRLASKRFDGRYADLGAAYFVADDPDFAALAESWRARGLARPWTDTFAVFPGEPTTGPVRWAAPGGLRSLAEDLAQGLDVTLSSTVETIPADADAVVLAMPGPQALRLDPPAAVADAARAQVWEPVLAAVCTYPERSWKDFRGAFVNDHPVLAAVWDDGDRRGDLAPVLVAHSTADFARPRLTDPAAAASELAAAVTELLGLDAQPAVHVHRWTYAQPSPGEPGPVTDGRVWLAGDAFGKPRVQTAWLSGRATARAILQSTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W008</accession>
    <name>U5W008_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glucose-6-phosphate isomerase</fullName>
            <shortName evidence="7">GPI</shortName>
            <ecNumber evidence="7">5.3.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phosphoglucose isomerase</fullName>
            <shortName evidence="7">PGI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphohexose isomerase</fullName>
            <shortName evidence="7">PHI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">pgi</name>
        <name evidence="9" type="ORF">AFR_20450</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7 8">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362734.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20450"/>
    <dbReference type="EnsemblBacteria" id="AGZ42362">
        <property type="protein sequence ID" value="AGZ42362"/>
        <property type="gene ID" value="AFR_20450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4147"/>
    <dbReference type="eggNOG" id="COG0166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017947_3_1_11"/>
    <dbReference type="OrthoDB" id="417261at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="PTHR11469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4 7 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="541" mass="59231" checksum="E67B3BB14F9129CA" modified="2014-01-22" version="1">MSEHVSDSAEWQAVQGHAEKFSSVHLRELFDSDPRRGERLTAQVADLYVDYSKNLVTDEVLAALIALAEKAKLRERIEAMFTGEHINVTEDRAVLHTALRLPRGAELVVDGQDVAHDVHEVLDRMGAFTDRVRSGEWVGATGERIKTVVNIGIGGSDLGPVMAYEALKADASIECRFISNIDPTDLYQKTHDLDPASTLFVIVSKTFGTQETLTNATEARNWLLAGLGGDQSAVAKHFVAVSTNAQRVADFGIDTENMFGFWDWVGGRYSLPSAVGLSVMLAIGKEKFGELLAGYHVVDEHFRTTPLEQNVPALLGLLNVWYDTFLGAQSHAVLPYSQYLHRFAAYLQQLTMESNGKHVRLDGAAVDYQTGEVFWGEPGTNGQHAFYQLIHQGTKLIPADFIGFSVPNHDTGEMHDLFMSNFFAQTGALAFGRTAEEVAAEGVPAQIVPHKVMPGNHPTTTILAEKLTPGVLGQLIALYEHITFTQGTIWEINSFDQWGVELGKVMANQLAPLLTAPSAPASSVDSSTDALIARYRAQRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0I6</accession>
    <name>U5W0I6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_16090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361575.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16090"/>
    <dbReference type="EnsemblBacteria" id="AGZ41500">
        <property type="protein sequence ID" value="AGZ41500"/>
        <property type="gene ID" value="AFR_16090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3265"/>
    <dbReference type="eggNOG" id="ENOG50327TI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2930638_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="60" mass="6180" checksum="E4581B246AEEB7F5" modified="2014-01-22" version="1">MDDTRSDLRDDQGTGAAIGRRAFFSRTLLAAGAVTGLLMLTGCPGSDGDDDDDDDGGDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Q8</accession>
    <name>U5W0Q8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363080.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22195"/>
    <dbReference type="EnsemblBacteria" id="AGZ42709">
        <property type="protein sequence ID" value="AGZ42709"/>
        <property type="gene ID" value="AFR_22195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4499"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051479_1_0_11"/>
    <dbReference type="OrthoDB" id="1079953at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="320" mass="33928" checksum="08F3788AF5E129EA" modified="2014-01-22" version="1">MITPSEALEAAALAALHAPSVYNTQPWRWRISGDTMTLHADHRRELAVADPDGRLLLLSCGAALQHARTALAAAGWQAAVERMPEPGVLARLTFTGEKPADPRAQRLAAAIVVRHTDRRAYAARPVPEPLLDGLRRIVEAEGAYLHVVRRDQVPLLAVAAEAAGNTELADPAYREELRQWTSRPAAAGDGVPATTAVRQELRRVPVRDFAPDGDAGLSAGDGHDTGSAYVVVFGSGDRPLNLLCGGEAMSALLLAATADGLATAPLSDTIEVAWSRQLMRDLLSGVGEPYLAVRLGYPADDAEPGTAPRRAPADVIEFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W106</accession>
    <name>U5W106_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_16445</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16445"/>
    <dbReference type="EnsemblBacteria" id="AGZ41571">
        <property type="protein sequence ID" value="AGZ41571"/>
        <property type="gene ID" value="AFR_16445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3335"/>
    <dbReference type="eggNOG" id="COG3940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009397_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14200">
        <property type="entry name" value="RicinB_lectin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665951" evidence="5">
        <location>
            <begin position="24"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="6">
        <location>
            <begin position="332"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="473" mass="52074" checksum="7E5E01E82C2AE07C" modified="2014-01-22" version="1">MKIFAVVGMVVALVAGLAPPAAAQFVVVGNPVVRQRADTAIYKHTDGYYYMTASVPDYKRVELRRSATVQGLATAPTVNAFTAPSSGSLSGWIWAPDIRFYDGAWYLYFSASPGSAVFDQRLYWIRNTCADPTTCAWSAPQRFTTGWESFQLDPASFVNRGVRYFVWAQDSPNTNYNSHMYIARMNGATGISGTVTEIARPTYAWEMEGVAGVVEGPSPLIRNGRVYIAYSASATDSRYKLGLLSSWDDADLLDPASWYKHPDPVFRTANGVYGPGHGSFTVAEDNTTDLLVYHARDYATPTPDALTDPNRHTRVQQLYWRENGDPFFGQPIPSTVRAPGIRGQHSSKCVDNYNFDTTPGALVRLWDCSGNTAQEFEFTYRGASGYEIRNRNTGTCLDDLGHNTAANADVGLYPCNGSTAQQWTIQDRGNGWFSLRNLAGNMCLDNDGWNTANGARLSLYPCTGVGAQLWRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W107</accession>
    <name>U5W107_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22795"/>
    <dbReference type="EnsemblBacteria" id="AGZ42829">
        <property type="protein sequence ID" value="AGZ42829"/>
        <property type="gene ID" value="AFR_22795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4620"/>
    <dbReference type="eggNOG" id="COG0510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105423_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="2">
        <location>
            <begin position="92"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="23099" checksum="059ACBC30F4989AB" modified="2014-01-22" version="1">MRPLRHGYTNDTRGDGSVVIKRYTGPDAPRRWATERDALETLAGRLPVPAVLAARTGELHLAHLPGVHGQELIDAGQAPAVLHSCGTMLRRLQSEGVVHGDYGPNNLLFDAHTYEVSAVLDWEWSHPGTGTIGDLAWCEWIVRTHHPAETGALPELFAGYGSRPPWNERRSAMLAKCRQMLALRRQLGTADPGYTRWEGHIATTSDWAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W146</accession>
    <name>U5W146_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_22995</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363380.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22995"/>
    <dbReference type="EnsemblBacteria" id="AGZ42869">
        <property type="protein sequence ID" value="AGZ42869"/>
        <property type="gene ID" value="AFR_22995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4660"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_3_4_11"/>
    <dbReference type="OrthoDB" id="2078574at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="19"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="11186" checksum="D8F470D667143F34" modified="2014-01-22" version="1">MSFDCTIERVGSTITVVPEGDIGVETVDAMREVLRQAVASLDGGHIDVDMRSVTFLDSTGLGMIVAARRAAAAKGIELTVSEPGPVVNMVLQIAGLDSVLVRKADDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1F1</accession>
    <name>U5W1F1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363353.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22885"/>
    <dbReference type="EnsemblBacteria" id="AGZ42847">
        <property type="protein sequence ID" value="AGZ42847"/>
        <property type="gene ID" value="AFR_22885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4638"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_1_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="283" mass="31152" checksum="AC3C17EBB4F699F4" modified="2014-01-22" version="1">MQREQLADFLRRRREAIRPAEVGLAGGPRRRTTGLRREEVALLAGMSVDYVVRLEQGRSSQPSTQLLGALARALRLSDDERDHLFHLAGHQPPPADGVARLARAGLLRMLDLLGDTPALVMSDLGEVLAQNRAALLLMSDLTGFSGDRRYIAYRWFTEPAARVTHPGAEADRHSRQLVADLRAVAGRRAADSTVTGLIDRLRAASAEFRRLWAEHEVGVRRADRKTIVHPRVGPILLDCETLVTPDLGQQLLVLTPADAEARECLELLRVLGLEEFPAGVPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W232</accession>
    <name>U5W232_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363642.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23920"/>
    <dbReference type="EnsemblBacteria" id="AGZ43052">
        <property type="protein sequence ID" value="AGZ43052"/>
        <property type="gene ID" value="AFR_23920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4845"/>
    <dbReference type="eggNOG" id="COG3795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130902_1_0_11"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="20"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="135" mass="14964" checksum="91D2F05159A97AAE" modified="2014-01-22" version="1">MAKFLLLKHYRGAPAAVNDVSMDKWTPEEISAHMQYMQDFADRLEKTGEYVDGQALAPEGMFVRYDGEGRPPVTDGPFAETKDLIAGWMIIDVDSQDRAVELAGELSAAPGAGGRPIHEWLELRPFLTELPTISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2F7</accession>
    <name>U5W2F7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362532.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19440"/>
    <dbReference type="EnsemblBacteria" id="AGZ42160">
        <property type="protein sequence ID" value="AGZ42160"/>
        <property type="gene ID" value="AFR_19440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3945"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_2_4_11"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="15448" checksum="9DD1301BC3113121" modified="2014-01-22" version="1">MGIADDAVEVRAQGWRRLAALHGLLETALEKALTSAVSLSVVEYTVMDALRRQDGWHMRMQQLGRAAALSPSATTRLVNRLEDRGLLTRVLCADDRRGIYTELTPAGHALLTQAQPIHDAALAEALARAEGVPELADLVVAASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2M8</accession>
    <name>U5W2M8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362533.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19445"/>
    <dbReference type="EnsemblBacteria" id="AGZ42161">
        <property type="protein sequence ID" value="AGZ42161"/>
        <property type="gene ID" value="AFR_19445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3946"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_1_11"/>
    <dbReference type="OrthoDB" id="1367386at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="17"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="30492" checksum="FD3CFEFE7F5D20CC" modified="2014-01-22" version="1">MPNTTLELAPDLTLTRMGYGAMQLAGPGVWGPPRDREAAIAVLRTAVELGITHFDTSDYYGPYTVNEVIREALHPYREGLRLVTKVGARRDPDRSWPPALSRQELTDAVHDNLKHLGVDVLDVVNLRVGAIDHPTAGSIAEPFGVLAELREQGLIRHLGVSMVTSEQLTEAQSIAPVVSVQNLYNLALRQDDTLVDRCAREGIAFVPFFPLGGFSPLQHGVLDAVAARLGASAQQVALAWLLQRSPTVLLIPGTSSVAHLRENVAAADLDLPADAIAELDGIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2M9</accession>
    <name>U5W2M9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_19815</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19815"/>
    <dbReference type="EnsemblBacteria" id="AGZ42235">
        <property type="protein sequence ID" value="AGZ42235"/>
        <property type="gene ID" value="AFR_19815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4021"/>
    <dbReference type="eggNOG" id="COG4097">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_19_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="224"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="452" mass="49464" checksum="0F5756673AE6D092" modified="2014-01-22" version="1">MTAANERLHPAPTRTALPPAQLARLVLWTVVGVNVLIVELVFLTAEAAKNPILGFGKWLGLHLALIMVLQLVLVARLPWLDRRIGMDRLTSWHRWTGFSLFWLVLLHPAFVLLGYANEYNSTPLTEFNNLKAQTPVLLGIVAAVIILVAAGLSVRAARRRLSYEAWHAVHICLYVAVVLGLIHQMFEVSTFTSTPLATAYWWALWAFALVSLVAGRVVMPLWRNARHQFRVAAVVPEAGNVTSVYVTGKDLDKLPARAGQFMIWRFPGHGHWWDANPFSLSAAPDGRTLRLTAKAVGSASAKLRDIPVGTRVFAEGPYGAFTSLSRTRESTLLIAGGVGITPIRALLEELTGPVTVLYRAHTTEDAVLLGELQNLARSRGAQLHLLTGRTGAGSPPNNPFDPQNLSALVPDIHDRDVYVCGPAAMTNAVLRSLRTLRLPTAQIHSERFSLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2P3</accession>
    <name>U5W2P3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative chemotaxis signal transduction protein CheW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25675"/>
    <dbReference type="EnsemblBacteria" id="AGZ43399">
        <property type="protein sequence ID" value="AGZ43399"/>
        <property type="gene ID" value="AFR_25675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5204"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129679_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="27"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="174" mass="18599" checksum="58C8B0454CAE46FB" modified="2014-01-22" version="1">MTDRLRELRGDFDRSFTEPARQHDVEYAELLAILAGGRAYALRLSQASGVHSDRPVTPLPGPQPALLGVAGFSGAIVPVYDLAALLGHPVPERPRWLVLAAGTPPLALAFHDLDGHVRVPTSTIVGETDGHSGRDVLRGMVPLPGGTRPIVDLPSARAVVHQLTGHTTHNTEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W303</accession>
    <name>U5W303_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chemotaxis protein cheV</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25665"/>
    <dbReference type="EnsemblBacteria" id="AGZ43397">
        <property type="protein sequence ID" value="AGZ43397"/>
        <property type="gene ID" value="AFR_25665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5202"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143963_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="16079" checksum="5D28085AFA530146" modified="2014-01-22" version="1">MAPPVGAPTGDSSLVFRAGTLLCALRLGEVVETMRPLVTHPLAGTPAFVTGICIMRGIPTLVIDVARLLGGAGADVERFIAVRTEHGPVAFATGAIHGVRPVSADSAHRHEALLGDAPARLVAAVGTIDAEPVLLLQSMRLVPDEVWAAAAALTVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W323</accession>
    <name>U5W323_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">M20_dimer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364455.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26680"/>
    <dbReference type="EnsemblBacteria" id="AGZ43598">
        <property type="protein sequence ID" value="AGZ43598"/>
        <property type="gene ID" value="AFR_26680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5407"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_11_2_11"/>
    <dbReference type="OrthoDB" id="906744at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="196"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="436" mass="46879" checksum="A920D4F3FC49FFB1" modified="2014-01-22" version="1">MEPLSAADEVVDICRDLLRIDTSNTGDPRTTVGERVAAEYVAGKLTESGIDVELHESAPKRANLVARIPGADRSRGALLVHGHLDVVPADASEWSVPPFSGEEKDGYLWGRGAIDMKDFDAMMLAVVRDWQRTGYVPPRDIVLAYTADEEAGMEFGSQFLVREHAGLFEGCTEAIGEVGGFSYTINDDLRLYLVETAEKGIDWLRLHAKGRPGHGSFVHDDNAVTALAEAVAAVGRHKFPMVITPAVRSFLEQVSEALGIELDPAEPELAIAKLGPIANLIGATVRNTANPTRLEAGYKDNVIPGKASATIDCRTLPGQAEIFLEQLRGVIGPDIEIEHISQQDALETGFDGALVEAMGAALRAEDPGARTVPYMLSGGTDAKAFSTLGIRCFGFAPLKLPADLNFSALFHGVDERVPVEGLQFGVRVLDRLLRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W333</accession>
    <name>U5W333_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364474.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26730"/>
    <dbReference type="EnsemblBacteria" id="AGZ43608">
        <property type="protein sequence ID" value="AGZ43608"/>
        <property type="gene ID" value="AFR_26730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5418"/>
    <dbReference type="eggNOG" id="COG1801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046519_1_1_11"/>
    <dbReference type="OrthoDB" id="1068600at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="PTHR30348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="SSF117396"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="28384" checksum="96FF4E9EC814E694" modified="2014-01-22" version="1">MLVIGTSGWQYRDWRGEDRLYPPKLPQRLWLEKYAESFATVEVNNAFYRLPERTTFAQWRDRTPDDFVVAVKMSRYLTHIKRLKEPAEPVARFLSRADALGSKLGPVLVQLPPTLKVDVAALDETLSHFPKEVKVAVEPRHESWWTDEVRDLLTRHGAALSWADRKGRPVTPLWRTTDWVYLRLHEGRARPWPHYGRTSLGSWLNRLPDEEAYVFFNNDPGGAAVSDAVTLAALARRRGRSVTRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3G0</accession>
    <name>U5W3G0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycerophosphoryl diester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27435"/>
    <dbReference type="EnsemblBacteria" id="AGZ43748">
        <property type="protein sequence ID" value="AGZ43748"/>
        <property type="gene ID" value="AFR_27435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5561"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030226_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666040" evidence="1">
        <location>
            <begin position="24"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="2">
        <location>
            <begin position="37"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="366" mass="40473" checksum="A0D89D572BB389E9" modified="2014-01-22" version="1">MLRFGALAAGVPAVLGAATPALATPQHEGHGRHDEDSLVVGHRGASGYRPEHTLASYELAARMGADFMEPDLVSTKDHVLVARHEPEIGGTTDVASRPEFAARKRTVVLDGVSVTGWFTHDFTLAELRTLRATERIPQVRQHNTLYNGLFQVPTFQEILDLRKKLSRELDREIGVFPETKHPTYFRRLGLDLETPLVRALRRNGLDRADAKVFVQSFEAKNLELLHDEYRLRAPMVFLTSAAGSPFDDTKTYADYLTPAGLRQLSRYVDGIGPEKNQIIPRNADGTLGTPTGLVADAHAADIKVMPYTFRAENQFLPVDYRVGTDPNAYGRAIDEQVTFLKTGLDGLFTDQPDIGVLARSVVFAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3K5</accession>
    <name>U5W3K5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thiol-disulfide oxidoreductase resA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_27240</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27240"/>
    <dbReference type="EnsemblBacteria" id="AGZ43709">
        <property type="protein sequence ID" value="AGZ43709"/>
        <property type="gene ID" value="AFR_27240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5520"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_13_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665511" evidence="3">
        <location>
            <begin position="28"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="40"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="19507" checksum="5CEB61633F11630B" modified="2014-01-22" version="1">MTVSKLKALIAAAAVTGLLAGCGSSPAAEPAADAALPVAAGTGASAPAAGVPGVVVPASLKFTGRTLDGKTFDATTMAGKPTILWFWAPWCATCASEAMSISDLQDEYAGRLNILGIAGMGNNKDMHEFVSDLEVGAVPHLDDEPGKLWKKFGITEQSTYVILDKAGKVVVRSYLDDLQLTDKVKSLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3L6</accession>
    <name>U5W3L6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21425"/>
    <dbReference type="EnsemblBacteria" id="AGZ42555">
        <property type="protein sequence ID" value="AGZ42555"/>
        <property type="gene ID" value="AFR_21425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21425"/>
    <dbReference type="HOGENOM" id="CLU_2204398_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="10845" checksum="28B703A1AEF46C82" modified="2014-01-22" version="1">MAGSGGAGGAAFFTGPFGAILRSSGENGSSSRPNRTTTQSSDSAGSVSATTWPTCPEICPADSVNITRGRRPAARPSRIVCNSSVLRPRVPVRAAVAMVVPSWSPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Y9</accession>
    <name>U5W3Y9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27940"/>
    <dbReference type="EnsemblBacteria" id="AGZ43849">
        <property type="protein sequence ID" value="AGZ43849"/>
        <property type="gene ID" value="AFR_27940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5663"/>
    <dbReference type="eggNOG" id="ENOG502Z8ZN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590316_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="466" mass="50440" checksum="A992092466D081F4" modified="2014-01-22" version="1">MEEFVTGPGERGLTMDRVMSHDGPERPLLEAASRAARGRSCDDLNRVPVLGRHGSLPDSLRRHALAHLDRAEAVPLEQLPHVDAASVAAWIAEHYPAGSYPGVVIGSAHGGAVHLAAALGAAWLPTTFTVTVPRDDSSAGDWAGAMAAGATAAGLILARNPQVSVRQVHDPVRRGPLCGSTITLHVRWRQLPPAYRHFLRTRLDPGAPVLLLRDTRSWPVVEPGPGHSFQLGSPASGLAPEQYSMEYAPFRRLMYSIGEQSWPVPGWNGLPRYAELAGEPELDRDLRRLCADSGRPVHRALYRGPDALSSCVADLYRRMLAAAGRNADRCVIESGRLVDPWRVLKSGLVPFWCEFPSRAAVDAAEWWLAGSAPFDSVTGLPEAPGTVCDTHADVAQWRAVSRFARHRPDLSRQAMGRYPLLPLATSHASTVIRGLEKQSPDPDPLTMPDVVTALRQAGSRLGFLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W403</accession>
    <name>U5W403_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitroreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22200"/>
    <dbReference type="EnsemblBacteria" id="AGZ42710">
        <property type="protein sequence ID" value="AGZ42710"/>
        <property type="gene ID" value="AFR_22200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4500"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051479_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="2">
        <location>
            <begin position="114"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="33929" checksum="292E80A805663327" modified="2014-01-22" version="1">MSGYTGDDLRRAATAAIRAPSLHNSQPWCFRLRDGTIEVLADRTRRPAVADTTGWALRLACGAATFNARLALAAAGTPADVLLRPYGELIATLTPARRRPPTYTERDLAAVIPRRHSNRRPFRPDPVPSETRVRLIEAARAEGARLELLVGTTALAGFGEIARSADRVLRRDPAYQAEQVTWTHADLAPDGVPVSAGGPPAEPQDLLPQRPYGERRRAPGRDFEPEPLVAVLATAADRPFDQVLAGQAMQAVLLTATDAGLATSLISQPIEVPAARDHLRRSLLRSGIPQIAVRIGYGDPGRPSPRRTVDDVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W458</accession>
    <name>U5W458_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42696">
        <property type="protein sequence ID" value="AGZ42696"/>
        <property type="gene ID" value="AFR_22130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4486"/>
    <dbReference type="eggNOG" id="COG3878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1204246_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="28871" checksum="AFCA01130D55783B" modified="2014-01-22" version="1">MLNHRTVKESTMAEWAALRRSSSAAGPFDAWLAPRSELTWRPAPEPVTGPVAKLGGQPVWLDEPFWPVSATSGEPMTFAGQFPLPGPDVRLAYLFLTQDEECLAETFEFEGGENALLIQPGGRIPGFVTGIPRATGPSLWRRGAKWTDRVPVELLLDVREPDAAARVRFDREIAGQDAVRTGVHREEDDDAEADCRSYVGGRPLFWQPWTTVVDSAWRFFFQLDGAEGADGDDFALNFGGGTGYAFLSEDGREGRFFWDCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W492</accession>
    <name>U5W492_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363257.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22650"/>
    <dbReference type="EnsemblBacteria" id="AGZ42800">
        <property type="protein sequence ID" value="AGZ42800"/>
        <property type="gene ID" value="AFR_22650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4591"/>
    <dbReference type="eggNOG" id="COG1099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061552_0_0_11"/>
    <dbReference type="OrthoDB" id="915852at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012022">
        <property type="entry name" value="UCP005295"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658">
        <property type="entry name" value="PTHR42658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005295">
        <property type="entry name" value="UCP005295_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR005295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="30799" checksum="5476CD9B34FFCFA3" modified="2014-01-22" version="1">MRIFDPHIHMTSRTTDDYRAMAANGVLAVVEPAFWLGQPRTNPGSFADYFDSLIGWEPYRASQFGIRHHATIALNPKEANDPRCRSVLNLLPRYLDKDGVVAVGEIGYDSMTPAEDEAFAVQLALAVEYELPALVHTPHRDKAAGTKRTLDVVAESGIAAGMVAVDHLNEVTVGLVKDTGCWMGFSIYPETKMSPPRMVEILQKYGTERVLVNSAADWGHSDPLLTVETGKAMLAAGFTADEVDQVLWRNPVEFYGQSGRLDLSSVSPEASFEGSSIKRGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4A1</accession>
    <name>U5W4A1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_22700</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363287.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42810">
        <property type="protein sequence ID" value="AGZ42810"/>
        <property type="gene ID" value="AFR_22700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4601"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_1_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041467">
        <property type="entry name" value="Sco4008_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17926">
        <property type="entry name" value="TetR_C_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="22677" checksum="39C3AE9DD479E563" modified="2014-01-22" version="1">MPEQPRSRRSPAGPERKRDAERTRERILDAALIEFGEHGYAGARIGAIARRAEANQQLISYYFDGKEGLYRALIQRWRSMSQELSRPDASLAEVVGAFTQVGETQRHWARLLIWQALEGDREEGGQEYRKAMVDDLRRRQEAGEIAADLDPGHLQLALFGAALAPTLLAHFAEDFTGKPADSPEFFAEYREQLGRLIGRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4F3</accession>
    <name>U5W4F3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28815</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28815"/>
    <dbReference type="EnsemblBacteria" id="AGZ44024">
        <property type="protein sequence ID" value="AGZ44024"/>
        <property type="gene ID" value="AFR_28815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5841"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30390" checksum="F8B00487D33E1D84" modified="2014-01-22" version="1">MTKRYGAKTVVDQLSFTAKPGQVTGFLGPNGAGKSTTMRMIVGLDAPTSGDVLVNGKPYVSSKAPLHEIGVLLEARAMHPGRTAVSHLLAIARTNGISRSRVDEVLGLVGLSAAAHKRVGAFSLGMGQRLGIAAALLGDPAVVMLDEPVNGLDPDGVLWVRNLLTGLAAEGRTVMLSSHLMSEVSLIADHLVIIGQGRLLADTTVADLMTAEGVRVVTAAPDELRGLIAGPGVTVTSTGSAEELFVAGTTARKIGLAAASRGIPLFELTPQNASLEEAFMELTHNAVEYEATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4N6</accession>
    <name>U5W4N6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28555"/>
    <dbReference type="EnsemblBacteria" id="AGZ43972">
        <property type="protein sequence ID" value="AGZ43972"/>
        <property type="gene ID" value="AFR_28555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5789"/>
    <dbReference type="HOGENOM" id="CLU_1182968_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="24306" checksum="20D70C9297300F71" modified="2014-01-22" version="1">MWTALATPLSVAIGEYFAYYDPQSDPERLEYHRDLAAHVYTAGHWFGQLVAALLGVWWVARSGAYRPATVLRAAATGAVLGLGTGAVAIAAGIRPMRANGYPSVAPDPTMLSDPAVRWCLLASLACFPLWTLIGAAITRLVRRGALLAVVAAILCQPVTLIFTVGVVAPPSSHSMAVATVADAGPTALLMPAILLAWAAVATGTATVVSRRRAGRPAEDPASPPEPPPDSPALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4P7</accession>
    <name>U5W4P7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADP oxidoreductase coenzyme F420-dependent</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560426.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44089">
        <property type="protein sequence ID" value="AGZ44089"/>
        <property type="gene ID" value="AFR_29140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5903"/>
    <dbReference type="eggNOG" id="COG2085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076368_0_2_11"/>
    <dbReference type="OrthoDB" id="1768241at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="2">
        <location>
            <begin position="2"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="23993" checksum="DE358F95163539C6" modified="2014-01-22" version="1">MKVGIIGAGNIGSALTRRLTAVGHEVTIANSRGPETLRDLAAETGAHAAPVTEAAEGADIVIVTIPQKNILDLPAGVLDGAAAGAAFVDTGNYYPRQRDGRIDALETGTPETRWVERHLGRPMVKAFNSILAQHLQELGRPAGAPERIALPVAGDDSAAKAKVSALVQELGFDAVDAGGLDESWRQQPGSPVYLADLDAEGVRNALTAASNDRPEEFRATASSPGTYADPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Q4</accession>
    <name>U5W4Q4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560329.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28655"/>
    <dbReference type="EnsemblBacteria" id="AGZ43992">
        <property type="protein sequence ID" value="AGZ43992"/>
        <property type="gene ID" value="AFR_28655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5809"/>
    <dbReference type="eggNOG" id="ENOG5031VNN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1923038_0_0_11"/>
    <dbReference type="OrthoDB" id="1874574at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021994">
        <property type="entry name" value="DUF3592"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12158">
        <property type="entry name" value="DUF3592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="131" mass="14066" checksum="82C0314A88C78142" modified="2014-01-22" version="1">MIFDGVLIVLAGAGVSCVGLVQLAKARRLRRTGVRSVGTVVSHEETTDDGSLYAPVIAFVDEHGSERRFTSATRTSWRVHPVGRKVPVIYPSGRPDTPRLISFGHDVLAVGLPLALGALFVFFGLLALIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4R1</accession>
    <name>U5W4R1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta superfamily-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44104">
        <property type="protein sequence ID" value="AGZ44104"/>
        <property type="gene ID" value="AFR_29215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5919"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_4_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="21"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="27598" checksum="D32CFDDFB73E7D9D" modified="2014-01-22" version="1">MHFVGDLPLAVNDYGGSGTDVLLMHGGGRTHHDWDEFAGLLRDGGFRPVAMDLRGHGESGPGRWSWDAAVADAEAVVAELGLRTPVVAGHSLGGMVAAVWATRHSDCPLAINVDGHGNPTRTDQLLGLTGEDAEVALRRLRTDLDGMSKGMSPALIQMMEEVDALDLPAVYRRRRCPMLVVSGSLTAEFALLFDEPSASAWRAGRDGMQARLNELACESGTFTHESLETGHDAHREDPEGLALLLMDHPALSMTSGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W535</accession>
    <name>U5W535_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phytochrome 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_30365</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560670.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30365"/>
    <dbReference type="EnsemblBacteria" id="AGZ44333">
        <property type="protein sequence ID" value="AGZ44333"/>
        <property type="gene ID" value="AFR_30365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6148"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025828">
        <property type="entry name" value="Put_sensor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13796">
        <property type="entry name" value="Sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="3">
        <location>
            <begin position="314"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="433" mass="46417" checksum="8E46B6E09B6996D4" modified="2014-01-22" version="1">MIVLRAWLTRRTWRELAYVLSGVLISVPTFALAVVGIVVAAASVLTVGLPLLIGVLALARLTPRYFRAHARWILGWRWDNPPPLPGRTLWRRILALPRDPAAWRALTYCFARFPVTVAGAYPAAFTVVAGPVALTYPLWWFLVPERFALVDTDTWGHTWVVALQGVAVVLVLPWWLRLIVLTDGFLTRALLHPSSAEQRIAELEAGRAALQADAAAMLRRVERDLHDGTQARLVSLGVTLSRIEHRSADEDVRKLAGDARGTVTEALAELRDIVRGMHPPALDDGLDVALSTLAARSAVPAEVTVELASRPPDATASALYFAVAELLTNIARHAHAHRVRIDLRTVGHHLRLTVTDDGRGGAAISPSTLRTDTGTGPGSSAAGSDGESRPRPAAARTGLAGLARRAAALDGSLTVDSPAGGPTTVTMTLPVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W537</accession>
    <name>U5W537_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23840"/>
    <dbReference type="EnsemblBacteria" id="AGZ43036">
        <property type="protein sequence ID" value="AGZ43036"/>
        <property type="gene ID" value="AFR_23840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4829"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056500_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="135"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="30954" checksum="29E07319A2DB8D12" modified="2014-01-22" version="1">MVAFSFTVPFTRVAVDSLSPLFIGSGRAVVAAVLAAAALAVTRQRLPHGTQWWRLAVVGGGVVAGFPLLTSYALTTVPATHSAVVIAVLPAATAVLVVLRGREHPPRAFWIAAGTGAVAAVAFASAQGGGFGHLHWADLLLFGAVLAAAIGYAEGGLLARELGPWQTVSWALVLCSPVMLALAVTSAIHEPPHATPAQWAAFAYLAVVSMFLGFFAWYHGLSLGPMAQVSQVQLVQPVLTITWAALLLGENLTWTTIAGGAAVVLCAGGAVRARISATAAENCRPQRRQSRGKIDELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W542</accession>
    <name>U5W542_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS family permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_29330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560464.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29330"/>
    <dbReference type="EnsemblBacteria" id="AGZ44127">
        <property type="protein sequence ID" value="AGZ44127"/>
        <property type="gene ID" value="AFR_29330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5943"/>
    <dbReference type="eggNOG" id="COG2807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038046_0_0_11"/>
    <dbReference type="OrthoDB" id="806182at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="16"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="410" mass="42367" checksum="2EA7A805A9CDDEA1" modified="2014-01-22" version="1">MSEHRPLPLWAGRTAALLGIALVALTLRQAVAAISPILGEIRVDIPISSIGVGLLGTLPPILLAASGFVAPRVARGIGLDGGILLALLLMTLGHLVRAFAPGFAVLLAGSVVALAGTGIGNVLLPPIVRRYFPDRVALLTAVYVCIVGVSTAVPAALAAPVAERIGWRFSLGMWSLTSAVALVPWLLLIVRERRRRLSDDLAAESAPTTLVARLWRSRVALSITVVFSTSTICTYAAFAWLPEILGDIAGSTPTEAGVLLAVTGIVSVPGALIAPLLVARLRNVGWLIAAGVASFVVGYLGLLLAPATLTLLWVLLIGSGSILFPVSLVLINVRTRTQGGTVALSGFSQGVAYALGALGPLIVGLLHDASGGWTLPLLFLLAVALVATVPAITLARPAFVEDQLGQSKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W593</accession>
    <name>U5W593_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_24420</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363740.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24420"/>
    <dbReference type="EnsemblBacteria" id="AGZ43150">
        <property type="protein sequence ID" value="AGZ43150"/>
        <property type="gene ID" value="AFR_24420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4947"/>
    <dbReference type="eggNOG" id="COG4521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_3_1_11"/>
    <dbReference type="OrthoDB" id="785019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010068">
        <property type="entry name" value="Peri-bd_TauA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024:SF38">
        <property type="entry name" value="PTHR30024:SF38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666099" evidence="2">
        <location>
            <begin position="25"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="35"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="35675" checksum="34B9F8C19ADBC0FC" modified="2014-01-22" version="1">MKKIIPALAAVLLLATTAACGNGAASGGSDSAEKSIRLAYQAFPSGDLIVKNQGLLEKALPDYKITWTKFDSGASINTAFLAKSIDIAAIGSSPVARGLSAPLNIPYQVAFVLDVAGDNEALVARNGSGVTDVAGLKGKKVATPFASTAHYSLLAALDRAGVKESEVTIVDLEPQDILAAWTRGDLDAAYSWLPSLDELKKNGKVLISSRELATAGKPTLDLGVVTTSFIQAHPEAVDAWRKVEAQALDVIANDPAAAGKAVGAELNLSPEDAQKQLSQGVFLEPADLASDEWLGTDAKVGKLADNLVSASEFLKGQQKIDAVPDLATVQKSIYVKGLPDVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5F9</accession>
    <name>U5W5F9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Poly(ethylene terephthalate) hydrolase</fullName>
            <ecNumber evidence="7">3.1.1.101</ecNumber>
            <ecNumber evidence="3">3.1.1.74</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_24770</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(ethylene terephthalate)(n) + H2O = (ethylene terephthalate)(n-1) + 4-[(2-hydroxyethoxy)carbonyl]benzoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49528"/>
            <dbReference type="Rhea" id="RHEA-COMP:12420"/>
            <dbReference type="Rhea" id="RHEA-COMP:12421"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:131701"/>
            <dbReference type="ChEBI" id="CHEBI:131704"/>
            <dbReference type="EC" id="3.1.1.101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:49529"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47348"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:50477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:47349"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.101" evidence="7"/>
    <dbReference type="EC" id="3.1.1.74" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24770"/>
    <dbReference type="EnsemblBacteria" id="AGZ43220">
        <property type="protein sequence ID" value="AGZ43220"/>
        <property type="gene ID" value="AFR_24770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5018"/>
    <dbReference type="eggNOG" id="COG4188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052605_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041127">
        <property type="entry name" value="Chlorophyllase2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12740">
        <property type="entry name" value="Chlorophyllase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666364" description="Poly(ethylene terephthalate) hydrolase" evidence="9">
        <location>
            <begin position="39"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ43220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="31166" checksum="4B3C49F7D84B4E21" modified="2014-01-22" version="1">MQSPTIPARLRARRLFVTAAATLTLVAGSLTGATAAQAAENPYERGPAPTSAVLDASRGPFATSQQSVSSLSVSGFGGGVIYYPTSTSAGTFGGIAISPGYTASWSSISWLGPRIASHGFVVIGIETNSVFDQPSSRGNQLLAALDYLTTRSSVRTRVDASRLAVAGHSMGGGGSLEAAKDRPSLQAAVPLAPWNTTKTWSGLQVPTLIIGGESDTVAPVSSHSIPFYNSIPSSAEKAYLELNNASHFFPQSTNTPTGRQAVAWLKRFVDNDTRYDQFICPGPSSSSISDYRNSCPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5J8</accession>
    <name>U5W5J8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363850.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24970"/>
    <dbReference type="EnsemblBacteria" id="AGZ43260">
        <property type="protein sequence ID" value="AGZ43260"/>
        <property type="gene ID" value="AFR_24970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5058"/>
    <dbReference type="eggNOG" id="ENOG502ZRFF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107599_0_0_11"/>
    <dbReference type="OrthoDB" id="1511245at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="23691" checksum="05EF4A1981FCF1F2" modified="2014-01-22" version="1">MRVLPDDQPWVFDLISPDDAVRAAARRRNQAVIAGASEALRRYNKVWTRPDFVPLAHAFVRETERTLKGLVSAFCSAEDPQVRARYAPYAVLYLQWEERYPAELTESWMWSIWTAKEVVLRNLHRQGVPPELGPEIADLIVAALHRPYRCKDWRYASLLHQVAPVERIVTIDDDRARFALHVVRNPAVNVTRATYPRWLAAQPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5T1</accession>
    <name>U5W5T1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Septum_form domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_30535</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560704.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30535"/>
    <dbReference type="EnsemblBacteria" id="AGZ44367">
        <property type="protein sequence ID" value="AGZ44367"/>
        <property type="gene ID" value="AFR_30535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6182"/>
    <dbReference type="eggNOG" id="ENOG5030VN0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080960_0_0_11"/>
    <dbReference type="OrthoDB" id="1953080at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026004">
        <property type="entry name" value="Septum_form"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13845">
        <property type="entry name" value="Septum_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666372" description="Septum_form domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Septum_form" evidence="2">
        <location>
            <begin position="49"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31334" checksum="C923867E74B7D7FB" modified="2014-01-22" version="1">MRRAPAPLVVLLVALFAAGCSATPPPGTDGDLTDDWAALATPAPFRPAAGQCHEALATTAPVDDYRPVDCAELHVSETFHVGTAADADVVPAPGSPGAKAAFRECSDVAADFLGGPWRSARVAVQVVWPTRSGWAGGARWFRCDVTTADLDGQSRTSREGSLAGELAGPSPLHLACFDPTVDGETVKTMLPVTCAEPHRAEFAGLWKAPDVAYAKLESDIDRSATGCRSVIARFAALPHNDDLQYRSGWISYNPTRTEWLSGERRVRCFIYFAERTFERSLKNAGPRVLPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5X1</accession>
    <name>U5W5X1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HpcH_HpaI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560896.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31515"/>
    <dbReference type="EnsemblBacteria" id="AGZ44563">
        <property type="protein sequence ID" value="AGZ44563"/>
        <property type="gene ID" value="AFR_31515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6379"/>
    <dbReference type="eggNOG" id="COG2301">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707531_0_0_11"/>
    <dbReference type="OrthoDB" id="674527at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH_HpaI" evidence="1">
        <location>
            <begin position="90"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="382" mass="41159" checksum="CF3F26D9DF4E907A" modified="2014-01-22" version="1">MRLSDDDYAALDGRLAAHDAFLAARYPGERPGRQPVHTVYVPADRIGGFREWGTQALAAIDGFPPLPFPAALADRVRAKLAAEPIEDLRVDFEDGYGVRDDDQEDAAVKAAVAVLLDGPRPPFLGLRIKSLEAPTRHRSLRTLDLFLSLYQEPFVVTLPKVSGPDQVTAMVTLCETLESRYALSAGTLRFEIQIELPSAVLGADGTATVARLITAADGRCTGLHYGTYDYSAAAGVAAAYQAMDHPVADYAKAVIQAAAAGTGVRLSDGSTNILPVGSPTAVHDAWALHHRLVRRSLERGFYQGWDLHPAQLPTRFAATYAFFADGRDAATTRLRRYLDRQSGGILDEPATARALAAYLLRGLDCGALDDVGFTREELGALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6H4</accession>
    <name>U5W6H4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative flavin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561151.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32775"/>
    <dbReference type="EnsemblBacteria" id="AGZ44813">
        <property type="protein sequence ID" value="AGZ44813"/>
        <property type="gene ID" value="AFR_32775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6633"/>
    <dbReference type="eggNOG" id="COG1853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_1_1_11"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="1">
        <location>
            <begin position="21"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="171" mass="18105" checksum="67E0CAE7A165E24E" modified="2014-01-22" version="1">MTVTPLHQDRAVDADLFRALLRRHAAAVVVITAPGEPPAGFTATSFTSVSLDPPLVSFCLAKSASAWPAVQAASTIAVHVLTQEQEHVARTFATRGIDRFAAHGAWRPGPDGVPLLEGVLATIVCKVAQHVEAGDHTIVLATPELGEHHADTTVTPLVYHDGTYAHLQRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Z6</accession>
    <name>U5W6Z6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MDMPI_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27470"/>
    <dbReference type="EnsemblBacteria" id="AGZ43755">
        <property type="protein sequence ID" value="AGZ43755"/>
        <property type="gene ID" value="AFR_27470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5568"/>
    <dbReference type="eggNOG" id="ENOG50314EV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094601_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017517">
        <property type="entry name" value="CHP03083_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024344">
        <property type="entry name" value="MDMPI_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11716">
        <property type="entry name" value="MDMPI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03083">
        <property type="entry name" value="TIGR03083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MDMPI_N" evidence="1">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="21918" checksum="C21AAE514FFC06F3" modified="2014-01-22" version="1">MWELVHAERAALAGDLKDLPGERWQTPSLCEGLSVREVLAHLTAAARLNGPRWLAGVIRCRFDFDKQVTMRLREQLGADGTETWERFRAVVTSTTKPPIPVPAMLGEMVVHGEDIRHPLGIRRDYPAATLAQVATYYSGTDMVVLAKGRIGGLRLVADDSPFTAGSGELVSGSTLALIMAMTGRAGHGGTLTGAGAEMLRARAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W700</accession>
    <name>U5W700_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27225"/>
    <dbReference type="EnsemblBacteria" id="AGZ43706">
        <property type="protein sequence ID" value="AGZ43706"/>
        <property type="gene ID" value="AFR_27225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5517"/>
    <dbReference type="eggNOG" id="ENOG5032QVU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130966_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="128" mass="13449" checksum="58EA39D707A446C9" modified="2014-01-22" version="1">MATIVALALLAVPRAVLHDLDVIHEGTVLNAVLVFVPLLIWVVVAVLHASNPFLALLATGCVYGLCLAVVHTVFWSPGTVDTAIPDLVLRGAVILSSLFTGIALGTVCGVVAWGLGRLRTSGCRRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W764</accession>
    <name>U5W764_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative AfsR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561179.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32920"/>
    <dbReference type="EnsemblBacteria" id="AGZ44842">
        <property type="protein sequence ID" value="AGZ44842"/>
        <property type="gene ID" value="AFR_32920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6662"/>
    <dbReference type="eggNOG" id="COG3903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052230_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="26984" checksum="35440326B2EB6338" modified="2014-01-22" version="1">MGSGEDIRLRQARAVVEAAEEHDRALCGPDEARAVAELDALLPDLRRAVEIARDDPALIGRLAGAVHRYGYHRQLPEVLAWGLYAAGSGCAAALAAAATCAWYRGDLAGARELLASAPADPAVHEVLGALALVAGDTGTALANFRAMAVLATDDAVRASGLAAQALVLARSAHPDEAVTTGGSAWEIAEGTGNPSARAAARHALGEALGDRDPLRALALLDEAADLARSVSNRLVLDAVTSAAAAIRSGAAREQRRIGPSDAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W787</accession>
    <name>U5W787_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Formate dehydrogenase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_27970</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27970"/>
    <dbReference type="EnsemblBacteria" id="AGZ43855">
        <property type="protein sequence ID" value="AGZ43855"/>
        <property type="gene ID" value="AFR_27970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5670"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin" evidence="3">
        <location>
            <begin position="12"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Molydop_binding" evidence="4">
        <location>
            <begin position="702"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="111"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="853" mass="95071" checksum="AC2014122A3F1D49" modified="2014-01-22" version="1">MAEAHPVGFQYVVDAKKRGAKVFHVDPRFTRTSALADTYVPMRAGADIAFLGGVVNYILSHGKDFREYVLAYTNASTMVSEDFQDTEDLDGLFSGFDPETKAYDQKSWAYAGQQEEGQDEEEAHTDRETASGLEQESHGPPVPADVPRDPTLQDPRCVYQVLKRHYARYTPEFVERTCGVPQDKFLEICEAWTANSGRERTTALVYSVGWTQHSVGVQYIRTGAIIQLLLGNMGRPGGGVMALRGHASIQGSTDIPTLFNLLPGYLPMPHYRQHPGFREWVDAIRHPEQKGFWAEAESYATSLLKAYWGDAATAANDWGYDWLPRLTGDHGTYQQVLDMIDGKIKGYFLLGQNPAVGSAHGKAQRLGMANLDWLVVRDLFMIESATFWKDGPEVATGEIVPEQCRTEVFFMPAASHAEKEGTFTQTQRMLQWREKAVEPPGDCRSELWFFYHLGRLVRERLAGSTLPRDQGILNLTWDYPLSGERGDEPSGEDVLKEINGYDTATGEPVPNFTALKADGSTACGCWIYSGVYKDGVNQAARRKPGSEQDWVAREWGWAWPSDRRTLYNRASADPEGNPWSERKKYVWWDPEAGEAGEWTGYDVPDFEKTKPPSYRPPEDATGVAAIAGDDAFIMQGDGKGWLYAPSGLIDGPLPTHYEPSESPVRNPLYAQQANPTRKVYDRPDNPINPSAPDERTGVFPYVFTTSRLTEHHTAGGMSRQLAYLSELQPEMFVEVSPELAAERGLTHLDWAHVVTARAAIEAKVLVTDRLAPLRIDGRVVHQIWLPYHWGGEGLVTGDSANDLIGITLDPNVLIQESKVGTCDIRPGRRPTGPELLAYVADYRSRAGLRAEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W795</accession>
    <name>U5W795_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33780"/>
    <dbReference type="EnsemblBacteria" id="AGZ45014">
        <property type="protein sequence ID" value="AGZ45014"/>
        <property type="gene ID" value="AFR_33780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6838"/>
    <dbReference type="eggNOG" id="COG3496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065913_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010775">
        <property type="entry name" value="DUF1365"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33973">
        <property type="entry name" value="PTHR33973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07103">
        <property type="entry name" value="DUF1365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="25622" checksum="D83886C2195214B5" modified="2014-01-22" version="1">MKPAVYESTVRHVRVSPLRHAFSYRTYQWLVDLDELPRNRWYASFEARDHVGNPQHSLRRNADEYLAAHGVNLEGGRILLLTNARTAGHVFNPLSVYWCRHRDGSLAAVIAEVHNTYGGRHRYLIEPGQTRVAKEFYVSPFFAVDGSYRMTLPEPDDRLALTVALDRAGGRPFVATVRGRRRSLRTAVLRNPFVTLAVAARIRFQGIRLYLRGLPVHPRPQETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7S2</accession>
    <name>U5W7S2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34615"/>
    <dbReference type="EnsemblBacteria" id="AGZ45179">
        <property type="protein sequence ID" value="AGZ45179"/>
        <property type="gene ID" value="AFR_34615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7003"/>
    <dbReference type="HOGENOM" id="CLU_1122722_0_0_11"/>
    <dbReference type="OrthoDB" id="18457at28056"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="27556" checksum="3B53F2B94B6FD8DE" modified="2014-01-22" version="1">MMSQRVRHHRRRNGLVDGVFVVVLMVGGLCGLIGLRVALLAFSDVAGGWADLLLFGVIGLAATAVWWWRQRIRRRAAQAIEQWERTAGWEPVLLGQRWPWVDMVRWAETVTVLRAYRKPGLIVGLVEFAENGLGAAVDRHAGRAAFAIVTLARPAPTGAVRVYREVAARRRGEDEFRRRFRVIGGELSPALQRAHVDGEVPAWTCTGDQMFVFVPLDGPLWPWHLERAAAKARRVVELSAVEVGEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W872</accession>
    <name>U5W872_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561749.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35785"/>
    <dbReference type="EnsemblBacteria" id="AGZ45413">
        <property type="protein sequence ID" value="AGZ45413"/>
        <property type="gene ID" value="AFR_35785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7243"/>
    <dbReference type="eggNOG" id="ENOG5033N0M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1097598_0_0_11"/>
    <dbReference type="OrthoDB" id="1584314at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="27054" checksum="CC35399FD213FB19" modified="2014-01-22" version="1">MDERTRYFRRLKRLRGSARRWSVLGGGLTAAAAVLTPYAGIGLADAAWAASAGVSVALAWWRWSDHRELAALPAPPAPEPAIPGQRLVAAVERFPAGRQVLQEVRRQKNRYALRGSAVALAWDRLDRASATLVGLAGRLTGAGEAAMLEAAVAEQWLRDLGQRVASVERAIPLSPPDQRPGIEHAHESLASQFTDGVTAYERLVAAAASYVAEEGHPATDQHPALTGLIEATDRLRGVAEGLSELRRPSTPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8B5</accession>
    <name>U5W8B5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription termination/antitermination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">nusA</name>
        <name evidence="9" type="ORF">AFR_35985</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Participates in both transcription termination and antitermination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561789.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35985"/>
    <dbReference type="EnsemblBacteria" id="AGZ45453">
        <property type="protein sequence ID" value="AGZ45453"/>
        <property type="gene ID" value="AFR_35985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7284"/>
    <dbReference type="eggNOG" id="COG0195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029242_2_2_11"/>
    <dbReference type="OrthoDB" id="384865at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_B">
        <property type="entry name" value="NusA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036555">
        <property type="entry name" value="NusA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013735">
        <property type="entry name" value="TF_NusA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010213">
        <property type="entry name" value="Tscrpt_termination_fac_NusA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08529">
        <property type="entry name" value="NusA_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69705">
        <property type="entry name" value="SSF69705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01953">
        <property type="entry name" value="NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="5 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 7" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="112"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="338" mass="36771" checksum="D8896B90B3E529B5" modified="2014-01-22" version="1">MNIDLAALRALEREREIPFETIIAAIETALLTAYRHTDGAESHARVEIDRKNGAAQVYAQEFDADGTMVREWDDTPHDFGRIAAMTAKQVILQRLREATDEVHFGEYAGRDGDLITGIVQADAARAEKGIVIVDLGKLEAVLPQSEQVPGESYEHGSRIRCVVVHVAKGFRGPQITLSRTHPALVKKLFALEVPEIADGTVEIAAIAREAGHRTKIAVHSRVQGVNAKGACIGPMGQRVRAVMSELHGEKIDIIDYSENAAQFVGNALSPAKALRVEVVDLATRTARVTVPDFQLSLAIGREGQNARLAARLTGWRIDIRPDNEPDTAVERDAAETGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9E2</accession>
    <name>U5W9E2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative amidinotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_37870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562163.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37870"/>
    <dbReference type="EnsemblBacteria" id="AGZ45828">
        <property type="protein sequence ID" value="AGZ45828"/>
        <property type="gene ID" value="AFR_37870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7658"/>
    <dbReference type="eggNOG" id="COG1834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057463_0_0_11"/>
    <dbReference type="OrthoDB" id="592329at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="28661" checksum="9BA4705E775B2FE8" modified="2014-01-22" version="1">MTTTAPTRRYLMCRPTHFAVTYRINPWMDPTAPYDTALAVSQWENLRQTFLDLGHTVDLIEGLPGLPDMVFAANGATVVDGKVLAVQFRDAERADEAPAYAAWFAGNGFEVFESKHTNEGEGDILLAGDVLLAGTGFRTSHAAHAQTQEVFGRPVITLQLVDPAYYHLDTALCVLDERNIAYLPSAFSPGSQAVLRRLFPDAVIATPADAEVLGLNAVSDGRTVVLPVQATALTAALQAAGYATIGVDVSELRKAGGGPKCCTLEVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9F4</accession>
    <name>U5W9F4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_37945</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562178.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37945"/>
    <dbReference type="EnsemblBacteria" id="AGZ45843">
        <property type="protein sequence ID" value="AGZ45843"/>
        <property type="gene ID" value="AFR_37945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7674"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="14"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="138"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="138"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="238" mass="26453" checksum="26AC79119BE0B63F" modified="2014-01-22" version="1">MAATQTQGKQSEAKLLVVEDDANILELLSASLRFAGFDVSTATSGSAAVSAAKNATPDLVVLDVMLPDLDGFEVIRLMREGGARTPVVFLTARDGTDDKIRGLTLGGDDYVTKPFSLEELTARIRAVLRRTTAGDEEPSRLVFADLELDEETHEVYRAGQRVQLSPTEFKLLRYLMLNANRVLSKAQILDHVWKYDFRGDDNIVESYISYLRRKVDNVQPRLIHTLRGVGYVLRKPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAL0</accession>
    <name>U5WAL0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33640</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561323.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33640"/>
    <dbReference type="EnsemblBacteria" id="AGZ44986">
        <property type="protein sequence ID" value="AGZ44986"/>
        <property type="gene ID" value="AFR_33640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6808"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_0_11"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001761">
        <property type="entry name" value="Peripla_BP/Lac1_sug-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00532">
        <property type="entry name" value="Peripla_BP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="13"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="36574" checksum="A4C6B98108CB53D7" modified="2014-01-22" version="1">MSAARSAEAARRPTLTQVAALAGVSLKTASRALNSEPNVAEETGRRVRDAADQLGYRLNGIARELRRGATSALVGLISGDLTNPFYSAVASGIERELRQHGLQLVTANNDEDAELEHELIDAFLERRVRALLVVPSADRHAYLAIEGSRGVPFVFLDRPPDGVAADVVVIDNAGGARAAAEHLLTRGHRRIAMVADLARMVPQRGRIEGFQQAMRAAGNSEWEPYLRTDVHDVRRAEQTVRELLELDPAPTAVFTTNNRLTTGALRALKEHPDPPALIGFDDFDLADVIGTTVVAHDAVVMGREAARLAVERIGGHAGPARSIVISTSVIARGSGERPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAN5</accession>
    <name>U5WAN5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33790"/>
    <dbReference type="EnsemblBacteria" id="AGZ45016">
        <property type="protein sequence ID" value="AGZ45016"/>
        <property type="gene ID" value="AFR_33790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6840"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081587_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="21912" checksum="6016B92FCAD7880E" modified="2014-01-22" version="1">MTTPELDISGPREHADAVVLVLHGGRATSVAPARRGLAWLRMVPFARAIAWSTRGRKVAVWTLRYRVRGWNGAAEDPVADTRWALEEARRRHPGARIVLVGHSMGGRVALRLAGEPDVVGVCALAPWIEAGEPAPKHSASVLIAHGEDDQVTNPASAAAYAARIGASFVPVPGESHALLRRPVFWNRLVTGFVTSALDVESHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB12</accession>
    <name>U5WB12_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Activator of Hsp90 ATPase 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562515.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39640"/>
    <dbReference type="EnsemblBacteria" id="AGZ46182">
        <property type="protein sequence ID" value="AGZ46182"/>
        <property type="gene ID" value="AFR_39640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8019"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112319_1_0_11"/>
    <dbReference type="OrthoDB" id="1914312at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="159" mass="18146" checksum="6CD2D702A1FDCD56" modified="2014-01-22" version="1">MTQEDPTAVRRQVVINAPLEKAFAVFTDRFGDIKPPEHNLLQVPLAETVFEPKVGGHIYDRGTDGSECRWARILAFDPPNRVVFSWDIGPTWQIETDPDLTSEVEVTFTAETPDRTRVELEHRNLDRHGPGWQSLRDGVDDTEGWPLYLQRYVAAFEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB54</accession>
    <name>U5WB54_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptidase S8/S53 subtilisin kexin sedolisin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_34550</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561503.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34550"/>
    <dbReference type="MEROPS" id="S08.069"/>
    <dbReference type="EnsemblBacteria" id="AGZ45166">
        <property type="protein sequence ID" value="AGZ45166"/>
        <property type="gene ID" value="AFR_34550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6989"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007528_0_0_11"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666236" evidence="6">
        <location>
            <begin position="23"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="7">
        <location>
            <begin position="202"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1168" mass="121776" checksum="9EBFB18E144C78B6" modified="2014-01-22" version="1">MLSRLLVGVLIGATAIPGPAAAAGAAPAPPPPPVPGAVRITLITGDTVELTKIDSRYAATVRPAPGRERITFHTIEADGGLRILPSDAIPLVGSGRVDAELFDVERLVAQGYGDAASPTLPLIVQGGGAALRTADAHPLTSINATAIRPAKSTLAGFWQTQSAAARTAGVTRIWLDGKVKPVLDRSTAQIGAPAAWQAGFDGRGVKVAVLDTGVDATHPDLAGKITEARNFSSSPDTVDRFGHGTHVAATVAGTGAGSDGKRKGVAYGADLLIGKVLGDDGYGYDSEIIAGMQWAVDAGAKVVNMSLGGEATDGTDPMSVAVDEISAATGALFVVAAGNEGADSTVGTPGAAPNALTVGAVDRSDQLADFSSRGPRLGDLGLKPEITAPGVEIVAARAAGTTMGNPVDELYTGASGTSMATPHIAGAAALLAQQHPDWTGPKIKEALVSTAKTTPDLSVYAQGAGRVDLTRATTQTVTGTGVADFGLHTVGETPAVATRTVTYSNASTAPVTLTLKAPAGVTTDTTTVTVPARGSATTVVSIDFGKRDPGRLSGWLTATAPGGVLVTTAIGATLDGPTHRITVKAVDRSGKPAAVPALTIHGDDSRSDVLGWFDPGGATYEVQAGTYLLDALLEDGGPLDEQANLTTIPELKVDKDITVLVDARKGTPIRIETPKPSEQQTVLSYYVHRVTGTGRSISHGVMHFSTIEQVNVVPTKKVTKGSFEFSSRWQLVAPMVQAKVPGVSGPLDINLFGTSPAYAGTKKFPLVRWGAAAVRGKAVLIPATDEDEMAQIDAAAKAGAAVALLVRPADQSAWTVYVPDAEERLPVVSLAVANDAGAQLLARAARPGATIDLTLTTSSPYLYDVLQVSKDRVPDRIVHRVTAANSMRIRSEYAHNGGLNWVREQRFGWRPWQTFAWNDTSRSVQTPSVREEWVSAGDSVWQHRVHSAYPWGGGVLRTGFTDRARSFAPGRTRETWSGPVVRPAAPAGISSTRTGDVLHLRVAEFVDSDGHYEEAGADEATATLWRDGKKLADLPDAWQDVTTTPGTAAYKLRLTTARGGDDWQFGTRTETEWTFRSGADGTLPLLHVGYDGRKTMLPHLLKLKFPAKLTSLKVDVSADDGASWKKAVTVGESALIPGGRKPVSLRVTATDRAGNTVTQTVIRAYGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB55</accession>
    <name>U5WB55_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_39840</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9517"/>
            <dbReference type="Rhea" id="RHEA-COMP:9527"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:9517"/>
            <dbReference type="Rhea" id="RHEA-COMP:9527"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562555.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39840"/>
    <dbReference type="EnsemblBacteria" id="AGZ46222">
        <property type="protein sequence ID" value="AGZ46222"/>
        <property type="gene ID" value="AFR_39840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8061"/>
    <dbReference type="eggNOG" id="COG4346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021079_0_0_11"/>
    <dbReference type="OrthoDB" id="1171867at2"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="GlyclTrfase_39-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="PTHR10050"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="PMT_2" evidence="10">
        <location>
            <begin position="108"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="PMT_4TMC" evidence="11">
        <location>
            <begin position="333"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ46222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="529" mass="58854" checksum="43063F45E397EDBD" modified="2014-01-22" version="1">MTSATAETDTTGPAPAEAPPDEAAATGLRGVPDAVRRRLATLDNWWNPYSWLITGLIVAVAAILRLVGLDRPKGYIFDEVYYPTDAWDMLQHGVEWDEKTNGPAYVVHPPLGKWMIALGEKIFGNNEFGWRISGAIAGTLMVLILIRVAYRMFHSIVLAGVAGLLMTLDGFQLVLSRTSLLDIFLGLFVLATFAALVLDRDHYRRRWLHALENGYDPATARRLPRVFPGWLLTAGVLFGLACGVKWSALFFAPFFAMLVVVWRVQARRSAGVRRPFLGGILGDLGYLLLSFALSIVFYLATWIGWFVTDTGYFRHYREANGLSEPPVLGALLNLMHYHAQAYSFHSGLTDRHVYQSWPWQWLLLGRPVAFYWNGNGNCGAPSCAAEILLLGTPILWWSFLPALAALVWFGIARRDWRAFAIGTGVVAGLLPWFYYAVADGRTMFAFYVLPALPFLILAVVYALGAIMTPPAGLVTGSGRTDRQLIGTVVVGVYVLLVALCFAYFYPIFVGQLLPYQDWSARMWLGGRWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB84</accession>
    <name>U5WB84_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TrmH family RNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562805.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41095"/>
    <dbReference type="EnsemblBacteria" id="AGZ46473">
        <property type="protein sequence ID" value="AGZ46473"/>
        <property type="gene ID" value="AFR_41095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8318"/>
    <dbReference type="eggNOG" id="COG0566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021322_0_0_11"/>
    <dbReference type="OrthoDB" id="1422015at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46429">
        <property type="entry name" value="PTHR46429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="2">
        <location>
            <begin position="130"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="372" mass="39090" checksum="2697C66AB0366F83" modified="2014-01-22" version="1">MPGNSFNVSKRMTSKKGASSGSGGKNRASLKGRGKTLPADERPWHKGYSGTEKLPEKTARKQDKERRAAAAEGRAPKIGTPGTKDTTWGRGGGRGTGKPAPQRGNRPQGRSGPRVAPGRKSNPTREGPELLLGRNPVTEALRASIPATALYVAQGIDIDDRITEIVRTAGDRGIPILEISRLELDRMTGGVLHQGVGLQVPPFAYENFDDLVAASLEQTAPLLVALDGVTDPRNLGAVIRSVAAFGAHGVFMPERRAAGITATAWRTSAGAAARVPVSQVVNLTRALKTAQQAGFTVIGLDADGETDLYQLEAAVGPLLVVVGSEGRGLSRLVGETCDLRVSIPMMSEVESLNASVAAAVTLAEVARRRIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBB0</accession>
    <name>U5WBB0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase GCN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40900"/>
    <dbReference type="EnsemblBacteria" id="AGZ46434">
        <property type="protein sequence ID" value="AGZ46434"/>
        <property type="gene ID" value="AFR_40900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8280"/>
    <dbReference type="eggNOG" id="COG0454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043786_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="178"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="315" mass="34535" checksum="4683A6D17FD085D4" modified="2014-01-22" version="1">MEPLTDNVKAFEIATAAFEVDAPGFPLDRREAFVARMQNPWPGNTLEHHAALLDGVTVGYVKIAMSQLDNLTSVDVEITVHPAYRRRGAGRALRDFAVARAREEGRKHLVAPASDGQPSGAAFATTVGAKAGLAEVRSRLDVAEVTGEPGPIAAGYRLVQWVNVAPEEFLDDIGYLDGRINEDAPNGDLVREAEKIDAARVRASEKAVRTRGRSAFNTGAVHEGSGRLVAWTMIASPDDAHEQAWQNATVVGPEHRGHGLGLTIKQENLRLARTERPALRVVDTFNAAENEHMIRINRQLGFRRMEAWTQWQLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBN3</accession>
    <name>U5WBN3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="1">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_41670</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562919.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41670"/>
    <dbReference type="EnsemblBacteria" id="AGZ46588">
        <property type="protein sequence ID" value="AGZ46588"/>
        <property type="gene ID" value="AFR_41670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8433"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_6_11"/>
    <dbReference type="OrthoDB" id="652304at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666493" description="Peptidoglycan glycosyltransferase" evidence="9">
        <location>
            <begin position="30"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="11">
        <location>
            <begin position="75"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="10">
        <location>
            <begin position="368"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="385"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="751"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="785"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ46588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="799" mass="85730" checksum="DD0319E3993FA3A9" modified="2014-01-22" version="1">MRRRDHNIFANATSLLICGLLAGVVVAAAAFPAVAMSGLAAKAGGETFASLPSELRRATAPQITRAFASDNKTQLSVFYDEFRSDVPLKDISKNMQNAIISAEDHEFYQHNGVDLKGVARAIVSNKSGKSQQGASTLTMQLVRMTLAYSATSPQEVVDATKDTPKRKITEMKYAMQIEKELSKEKILEGYLNAAPFGNGAYGVYAASQVYFSKKPKDLTVAESALLASMVKAPTAFNPTTASGYPQALDRRNYVISDMQELGFVTEADAKKAVATKIKRTVKRPGNGCVSVAKNNWGFFCDYFYRWWLSQDAFGATTYDRERRLKSGGYRIQTSMDIKAQQAARENIGDHISEKNQNALLLAGIQPGTGRVRALAANRKFKLDNPDKPVNKISSDPKKAKKKIRGTYPNTTNPLLSGGGDITGYQAGSVFKMFTMVAALENGYPLAYSINAPAKAVTKYRIAPGRSTCNGIYYCPSNSGGKAGGITNMWTGFSRSINTFFVPLQERVGAEKVVDVAQRFGVQFRAKNDADFASNKASAHQWGAFTLGVSSSTPLEMANAYATLAGDGMYCAPTPVQQITSKSGEKIDVGKPHCTRATSPDVARAALDAARCPVGDNAQLGSCGSSRTAARAYQTIRHPIYGKSGTTDNDKTAALIVGSSSLTIAGYLVNPDYQDHKDRMSHDIVNPAVWDTMDDFLKGKPRVQFKKPGSSKISIGDQRSIPDVTCATLAGAKSRIEGAGFSASVGQEVESKCPKGTAAGTNPSGRTIKNGYVGIEISKGPGKDPAGPDDPNKPKPPGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBY8</accession>
    <name>U5WBY8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative pyridoxamine 5'-phosphate oxidase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562839.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41265"/>
    <dbReference type="EnsemblBacteria" id="AGZ46507">
        <property type="protein sequence ID" value="AGZ46507"/>
        <property type="gene ID" value="AFR_41265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8351"/>
    <dbReference type="eggNOG" id="COG3576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123922_3_0_11"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019920">
        <property type="entry name" value="F420-binding_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03618">
        <property type="entry name" value="Rv1155_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="5"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="14451" checksum="667A05DBCB489401" modified="2014-01-22" version="1">MTTALPDKVRKLLDGPTFVVVTTVNADGSPQSTVVWVKRDGDDILFSTVRGRKKTRNMERDPRISICAYDPEQPYSYFTVDGSVALVEEGGKELINELSLKYDGQPYTFDPPEAVRVVCRLTPRKVISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC08</accession>
    <name>U5WC08_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561827.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36175"/>
    <dbReference type="EnsemblBacteria" id="AGZ45491">
        <property type="protein sequence ID" value="AGZ45491"/>
        <property type="gene ID" value="AFR_36175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7320"/>
    <dbReference type="eggNOG" id="COG1233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019327_0_1_11"/>
    <dbReference type="OrthoDB" id="453021at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="1">
        <location>
            <begin position="228"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="531" mass="55486" checksum="8D544203D50E7AA1" modified="2014-01-22" version="1">MSALPDRADVVIIGSGHNGLVSAVLLARAGLDVVVLEAADVIGGATRTEHPFPAVPGLGQSTGSYLLGLMPPELLRTLDVDIPVLRRDPHYFLPTPGGPGSPYLLFGTDREATRAQMERFFSPRDVAADEAMQVEIAALRSDLAPAWLQEPGSVEEIADRHIRPQLQSTFIDLVRGSVADYLTRFGFKSELLMSMYAVTDGLSGLNAGPDDPGTGHNFLVHNMCRLPGSDGTWMIAAGGMGTVSRTFADAARAAGASIFTTSPVSAVTVSGGAATGVVLADGRSVSAQVVLGACDPYQLMSLLPSGALPSSLTTRMEAVRRPGTTLKVNLALRGLPRFACLPADAPSPFGSTIHLLPGSAGLAGLAETDSPMAALRAMWADVQAGRLPAEPTIEWYLHTTVDPSLQDAAGHHSSALFVQSVPYALADSSWDAELPGYVARLLDIVDRYAPGTSDLVADTMPLTPAGIEKHFGITGGHIHHVDNTVSFNERMPYATGLDGLYAGSAGAHPAGSVIGAAGHNAARRILKDLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD55</accession>
    <name>U5WD55_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Succinyl-diaminopimelate desuccinylase</fullName>
            <ecNumber evidence="2">3.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562289.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38505"/>
    <dbReference type="EnsemblBacteria" id="AGZ45955">
        <property type="protein sequence ID" value="AGZ45955"/>
        <property type="gene ID" value="AFR_38505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7791"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_1_0_11"/>
    <dbReference type="OrthoDB" id="906744at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009014">
        <property type="term" value="F:succinyl-diaminopimelate desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010174">
        <property type="entry name" value="Succinyl-DAP_deSuclase_DapE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01900">
        <property type="entry name" value="dapE-gram_pos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="170"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="358" mass="38697" checksum="9DBED4BC93759127" modified="2014-01-22" version="1">MANPLTPDVLVDPVELTRALVDIESVSRDETVIAGHVEDVLRQAPHLSTERRGNTVMARTSLGREQRVVLAGHLDTVPLNDNFPSTVVDDLIYGCGASDMKSGVALALHLAATLPEPGYDITYLFYEAEEIESQYNGLQLISESDPEWLQADFAVLLEPTYGVVEAGCQGTMRVLVRTTGRRAHTARAWRGVNAIHAAGEVLRRLDDYRARTVTIDGCTYREGLSAVRITGGVAGNVVPDECAVEVNFRFAPDRTEKQGLEHLHEVFAGFDLEVTDSAPGALPGLTAAPARDFLIAVGAEPAAKLGWTDVSRFAALGIPALNYGPGDPLLAHAQDEHVEIGKIRDGAATLHRWLTAYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE49</accession>
    <name>U5WE49_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39735"/>
    <dbReference type="EnsemblBacteria" id="AGZ46201">
        <property type="protein sequence ID" value="AGZ46201"/>
        <property type="gene ID" value="AFR_39735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8039"/>
    <dbReference type="eggNOG" id="ENOG502ZC7T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034272_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="555" mass="59914" checksum="A69A925EEABC0655" modified="2014-01-22" version="1">MSAGVAVSPAAAAAPQYKFTLLAKAKADQCFAGVGQPYPAGPPCRTGQSKVNQAYVWGLTQVGKMIWFGTGANVHCLVSGATLDYVKPTANDNWTCEYGESQHAKTDPAIPEYLGDMRAPEVWTYDTATGRSVNKSAEIRAKSPEDATRLAHTLGLRAAGNFGNVVLLGGPALENTLNMFAFDATTKQYLGSVNFPDYGNIRTFLVAEDALYLGVGIGRNGSAGGAVWRWNGSTTDPFAFQTVAALPVQAADLTVYDGRIAATSWPAEQPTTQQMLAGLWISPKLADGTPGLNPEDSAGWKQVWNARLYEPDRVISATYGGGGIAAFDGYVYWGTMHVPMKSSTVHATVYPPVDDQAAKDQAVKTQRAVSIWRGKDLGLPTQKIELLYGEKTLPAWDPATSTWSDKSTNYTPLYGKSGFGQLFNNYTWRMTVVDNKLFVGTMDWSYLVKDLLAGAAPPVNAKARKALADPDNWHKPLVSPANFGGDLYMFPSSTEPATAVNTRGLGNYLNYGIRNMIPNGKGGLYIGMANPMNLRTNKTDARPEGGWELLEMNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE93</accession>
    <name>U5WE93_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_39960</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562579.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39960"/>
    <dbReference type="EnsemblBacteria" id="AGZ46246">
        <property type="protein sequence ID" value="AGZ46246"/>
        <property type="gene ID" value="AFR_39960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8085"/>
    <dbReference type="eggNOG" id="COG2095">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079909_0_0_11"/>
    <dbReference type="OrthoDB" id="1323133at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="PTHR33508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00427">
        <property type="entry name" value="TIGR00427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="21122" checksum="675D8AE20254D5D7" modified="2014-01-22" version="1">MNLKLFGEVFVTLLVIVDPPGMVPVFLALTGTLPVKERIRAGTQAVLLALGVIVIFAVAGQTLLDYLHVQLPALQGAGGLLLVLVALQLLTGKTDEPSEQAGTSNVALVPLGTPLLAGPGAIVATMLFVQRADGLNDYTVIGLGIIAVMATVWLVLRFSGGIVKLLRPGGIEVLTRIAGLLLAAIAVQLIADAVAAFVELYTAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFL9</accession>
    <name>U5WFL9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_42905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563165.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42905"/>
    <dbReference type="EnsemblBacteria" id="AGZ46835">
        <property type="protein sequence ID" value="AGZ46835"/>
        <property type="gene ID" value="AFR_42905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8680"/>
    <dbReference type="eggNOG" id="ENOG502ZDF8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929483_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="32320" checksum="2B87353F4509340C" modified="2014-01-22" version="1">MVVVPVSEALRRLSEDPRFWSFLFVNDGAHPDPDPAEVRVSLPVTGGYGLVLDLDLVTGEQTLGLREPASSEPVQLGWTGPGRPYPAALRWHELELCARVIALEDPTLPHPGLVVALLSPFAPLTPDDDVDAVAAVREAAYRSLRREVPPVAAAGPEQAPLPLFTAEAWWPRPPALSPQVIDEAAVAAYTVPAPVQLEVRGGSRFPREGLTELVRQAAQRLSRLPEEQLYAEVRPLARHIADTGDLRPVNDLLGVLTEAGCDHPTVLDALSEPLVPIEACWMVETLAGAVPGSLLRRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNS8</accession>
    <name>U5VNS8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00800"/>
    <dbReference type="EnsemblBacteria" id="AGZ38449">
        <property type="protein sequence ID" value="AGZ38449"/>
        <property type="gene ID" value="AFR_00800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.160"/>
    <dbReference type="eggNOG" id="COG2339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056942_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026898">
        <property type="entry name" value="PrsW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36844">
        <property type="entry name" value="PTHR36844"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13367">
        <property type="entry name" value="PrsW-protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="431" mass="45610" checksum="DC89EFAE0837068E" modified="2014-01-22" version="1">MTAEPRLRSARTAPVQPGDTPGLGLMRLPAFWVVLAVLVAGGVRIAMIGGRFAGTYPLATLTAVALFALLAVPFWFVVSELDFLEREPPALMALAFAWGGLVATSVSIPGSAALDDLIAKLGSPHLAADWGAALAGPTVEEIAKTLGVVAIVLVARAQVNSVLDGVVYGAMVGLGFQIIEDIVYALGAVALAGRGDHIEPVLTTFLLRGFLAGLWSHTLFGALAGAGIGYLVVRADRGWHTRLGVAMLAVFGAWASHVLWNSPLFRDGLGNGALALLAVLVFKGLPPLLLIMVLVRAAHDREADYYAAQLATLDDPELITPDELEALKAGSRRASARWHARTRAGFRARAAVRRLQRAQAQLAVELSRGRTDLEPWCHEVRRQRRILAGLGHPEAIAPEGKGPWRRTASTVFTTALAIAVLWAAISTLGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPU9</accession>
    <name>U5VPU9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357772.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02700"/>
    <dbReference type="EnsemblBacteria" id="AGZ38829">
        <property type="protein sequence ID" value="AGZ38829"/>
        <property type="gene ID" value="AFR_02700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.543"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_11"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="25016" checksum="333937611FC542E6" modified="2014-01-22" version="1">MSERVALVTGASRGIGLAVARRLAEQGHDLVVHGHRADVIAEVAAELAGKYAVTVVPAHGDIADPETSRVLMRVAFENFRRLDALVVNAGTHAAGVLGMTDDATVQRLFAVNAIGAAHTLQGAVRLLGRGRTPAVVLVSSITGTEGAAGQAIYAASKAAVSGLALSAAKELGPRGIRVNAVAPGFITTDMLDTLDDRGRAQRIASTALGRLGEAGEVADVVAFLLSDQARFVTGQVLGVDGGLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ96</accession>
    <name>U5VQ96_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GGDEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357930.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03490"/>
    <dbReference type="EnsemblBacteria" id="AGZ38987">
        <property type="protein sequence ID" value="AGZ38987"/>
        <property type="gene ID" value="AFR_03490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.701"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_30_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="339"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="468" mass="50295" checksum="3022E7324377E285" modified="2014-01-22" version="1">MSRAGVWIFLVVTFVVAAVEVAVYFRLAGEIVHVTAYVLLCAVAWWSAFRRHEGRDRRIWLLVAACQTLWFTGDAYELLELHLLGETSAVGPADVFWLGPYPLLATALALMARRRAPGRLRSSVVDALTLTVAAAAVSWPFVMKPLTDGATFAEAAVPLLYPIGDVVLLTAVLLIALSPGARTAPTRLLLAGVVWYLLMDVGWTALPYLVSEDIYGRLNAAMLIGNALITAAGLHPARAELVSPGRASRTLHPARVFFLAVALLTPPVLNVFIRPDLVGLLASVVCALLVVVRFVLVVREQARTQEQLAHQADRDPLTGLANRSVLGDRLARTVPAPDAPVAVLYLDLDGFKEINDQYGHEAGDVVLRTVSERLTVAVREGDLVARLGGDEFVLLCPGVSAEEAVTLAERIISDVARPVAFEGHHLVVGGSIGIAARTEPAAEGLLRSADAAMYEAKRLGRGRWILAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQM3</accession>
    <name>U5VQM3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_03990</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358032.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03990"/>
    <dbReference type="EnsemblBacteria" id="AGZ39087">
        <property type="protein sequence ID" value="AGZ39087"/>
        <property type="gene ID" value="AFR_03990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.806"/>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007891">
        <property type="entry name" value="CHASE3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05227">
        <property type="entry name" value="CHASE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665589" description="Histidine kinase" evidence="8">
        <location>
            <begin position="34"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="217"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="305"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="257"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ39087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="531" mass="58613" checksum="E8D2464FF4CDEB74" modified="2014-01-22" version="1">MSLLSVRSWTLRRRVVALCLAVGLVLTVLGVFAAITAADSNDHIDVILNKTGPMRAAGESLNTAVVNQETGIRGFAISGNENNLRPYTDGLAAQDQLLRQIGSLNEQSGNGDPKVRAALLVVQQRADAWRAVVADPVINAVRTQGPDAGQELVEAASTREFDALRTSITDLQDEIWIVRNKAADAAKRTGTVLVSVEIFAAVVVILAGAALLLLLDRLITRPVTELAEQVRDVARGNYDKHITSEGSPELRLLANDVDGMRQQIAAELTEVREARQQIEWVNDQLKLQAEELTRSNRDLEQFAYVASHDLQEPLRKVASFCQLLQRRYAGQMDERADQYIAFAVDGAQRMQRLINDLLAFSRIGRLTAGFTDVDLDHVMTEVKSQLEVRAGDDGEITWADLPTVEGEEPLLTTLLVNLVGNSLKFRRPDVPPKIHVTAEREEKEWRINVRDNGIGIEAEFADKVFVIFQRLHARDAYEGTGIGLAIVKKIVEYHGGRIWLDLEVAEGTSIYFTLPLLAGVPVQEPLKEVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQU5</accession>
    <name>U5VQU5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucanase</fullName>
            <ecNumber evidence="8">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_05270</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase family 6.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05270"/>
    <dbReference type="EnsemblBacteria" id="AGZ39343">
        <property type="protein sequence ID" value="AGZ39343"/>
        <property type="gene ID" value="AFR_05270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1066"/>
    <dbReference type="eggNOG" id="COG5297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015488_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016288">
        <property type="entry name" value="Beta_cellobiohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036434">
        <property type="entry name" value="Beta_cellobiohydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001524">
        <property type="entry name" value="Glyco_hydro_6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34876">
        <property type="entry name" value="PTHR34876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01341">
        <property type="entry name" value="Glyco_hydro_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001100">
        <property type="entry name" value="Beta_cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00733">
        <property type="entry name" value="GLHYDRLASE6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51989">
        <property type="entry name" value="SSF51989"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00656">
        <property type="entry name" value="GLYCOSYL_HYDROL_F6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 8" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="4 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="6 7">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001100-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="348" mass="36951" checksum="EC39420A433CA0BC" modified="2014-01-22" version="1">MRRHWPLIAAGLSLTLVLTAIVTLSMRGDDSPVVVGPAAAQKPVPTPSRAGRLADRLLYVDPQGAAIEQAAEWERAGRTEDARTVRRIADQPTATWFADDSPGYAQRARQLVTAATAAGRLPVLTFYNIPYRDCSGQSAGGAADAEDYRSWVRALAASLDGHRALIVLEPDAIPQAVQGCLDAEDTAERYTLLAEAVDALRALPGVLVYLDAGNPTWIEPGPMTTALRRSGIERAYGFALNVANFETTEDNLAYGARLSTLLGGTHFVIDTSRNGNGPAQKGAGDRHWCNPSGRALGETPTTRTGVPLADAFLWIKRPGESDGACGSGAPPAGQWWPEYALDLAGPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQX6</accession>
    <name>U5VQX6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04800"/>
    <dbReference type="EnsemblBacteria" id="AGZ39249">
        <property type="protein sequence ID" value="AGZ39249"/>
        <property type="gene ID" value="AFR_04800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.969"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="35"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="395" mass="42354" checksum="FE9A5A1C289E14A4" modified="2014-01-22" version="1">MTTSTPADPLVGRMRAFGTTIFTEMSALAARTGSVNLGQGFPDTDGPPEMLAAAAEALRSGANQYPPLPGIPALRAAIVEHEHRFWGLSRDPDTEVVVTAGATEAIAASILGLCEAGDEVVCFEPYYDSYAASIALAGAVRRPVTLRPGPDGRYGFDEDELRAAFGPRTRLILLNTPHNPTGKVFTADELTLIARLCQDHDVYAVTDEVYEHLVFTDAAAPHIPLAGLPGMRERTLRISSAGKTFSCTGWKVGWATGPAPLVSAALRVKQFLTFVNAGPLQPAVAVALGLPDSYFEGFRAGLQSRRDRLVAGLTDAGFHVLPSDGTYFVTADITPLGGTDGVDFCRALPERCGVVAVPTQVFYDHQEQGRHLIRFAFCKREDVIDEAAKRLRTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRM5</accession>
    <name>U5VRM5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06125"/>
    <dbReference type="EnsemblBacteria" id="AGZ39514">
        <property type="protein sequence ID" value="AGZ39514"/>
        <property type="gene ID" value="AFR_06125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1244"/>
    <dbReference type="eggNOG" id="ENOG502ZBKE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062173_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025683">
        <property type="entry name" value="Protein_beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14350">
        <property type="entry name" value="Beta_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="335" mass="37092" checksum="AAC00858EFE5B6DA" modified="2014-01-22" version="1">MTDGVYRPILRPRRGELAALHHLDADLAQRVMPIIELAPDDRLPQLVRQMPPRTGAIAVDLGAIADPADTLSSPPLDLAEELADVGVAMLPVLRAYDSRRRLVEHGLAARMHLMRAVLRLQPHLDARNATAATKLTDRMLAGAGLEAERVDLVIDLAETACAAHADRFEERARNVLRWARAEDWRSVTVAAGAMPPNLDDLPTDEPVAVGRLDAQVWARLSEPGVGYADYGVTSPVRRHGINHRQLPTLRYTAEHDWWIYRWARRGGRSDDRCHDLCRTLVDSPQWPSTGARFSWGDAQIARQSRSARGGGSPASWIAWSTSHHIAHVLRTIAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRV5</accession>
    <name>U5VRV5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thymidylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tmk</name>
        <name evidence="5" type="ORF">AFR_07070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359234.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07070"/>
    <dbReference type="EnsemblBacteria" id="AGZ39703">
        <property type="protein sequence ID" value="AGZ39703"/>
        <property type="gene ID" value="AFR_07070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1443"/>
    <dbReference type="eggNOG" id="COG0125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092360_0_0_11"/>
    <dbReference type="OrthoDB" id="1585072at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate_kin" evidence="4">
        <location>
            <begin position="25"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="25"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="29291" checksum="D8E7FB7F58A1B950" modified="2014-01-22" version="1">MVPDLESRPARQSGVPRLRTVALVGIDGSGKTTQAHLLAEGLAARGLPATYRRNAGGRRWFGRLATTLGRRDGEDLLGRKAMLLVESVLRWLAILRTLLRRKVTGEVAVMDRYAVCQYASIRAHAKNPRPERINRAERRARLAYRVFPKPDVTFLLAVDPEIAYDRIERRGYDHEEMDYLRAATAAYEALPEHRDFVVIDANGTPAEVEQALLHHMRTWTPPVTTTVPAPRTAALVSHARALIVAVATLAAAASVLTYQLAEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VST4</accession>
    <name>U5VST4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH-type transcriptional repressor purR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02455"/>
    <dbReference type="EnsemblBacteria" id="AGZ38780">
        <property type="protein sequence ID" value="AGZ38780"/>
        <property type="gene ID" value="AFR_02455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.496"/>
    <dbReference type="eggNOG" id="COG1879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665645" evidence="1">
        <location>
            <begin position="26"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_4" evidence="2">
        <location>
            <begin position="83"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="361" mass="37943" checksum="1A5800AB35DA2C4D" modified="2014-01-22" version="1">MSGRWRPAVLTLGAALLLASASACAKSEDSDTAGGSGTSASAGAQVAQSAAPGAKSCTLEQFGGTKIDLKTAKVGFSQSEKEANPFRIAETKSIKDEAAKLGIPAGNLQTTNANSQFNKQISDVEQMIDSGVQLLVIAPLNSDGWDSVFAKASAKKIPIITIDRKINATSCTDYLTFIGSDFEEQGKRAADKMAAALGNKGTVAILLGAPGNNVTTLRTKGFKDQIAKVAPEIKISFEQTGQFSREEGQKVSEQLLQSNSDINGIYAENDEMALGAEVALKGAGKKAGDVKIVSIDGTKGAVQGIVDGWIYSVIESNPRFGPLAFETATKFFNGEPIPEDIIISDREYDESNAKTDLNSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VST9</accession>
    <name>U5VST9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase_C_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_02085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357649.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02085"/>
    <dbReference type="EnsemblBacteria" id="AGZ38706">
        <property type="protein sequence ID" value="AGZ38706"/>
        <property type="gene ID" value="AFR_02085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.422"/>
    <dbReference type="eggNOG" id="COG2378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013420_0_0_11"/>
    <dbReference type="OrthoDB" id="1388804at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032830">
        <property type="entry name" value="XPB/Ssl2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13625">
        <property type="entry name" value="Helicase_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase_C_3" evidence="4">
        <location>
            <begin position="513"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="811" mass="84645" checksum="33FF22609089A5BF" modified="2014-01-22" version="1">MATAFADQLRSLTDDALGALIQLRPDLVVPVPADVSALAVRAQSRGSVSRCLDTLDEFTLAVLDAARLSRAVETAQTSVAAILELTTGSADPADVRVAIDRLRARFLLHGPEEALQVVGAVDEVTSPYPAGLGRPADYLDAQAAALCADKAKLRRTVLAASPGARAVLDRLAAGPPVGALSSNGSVAEPVQWLIDNHILVQVSEAGRAPRADGELVELPREVGILLRRDTGPLGQLRPVPPTPDSPVRDPKAVDSAGAGQAMEAVRHVETLLESLSGEPAVVLKTGGLGVRDLKRLARAANLDEPSAAALIEIAYAAGLLGEADAAGTGRGVTTSRASGLVDEIFLPTSAYDLWRALSIAQRWEALANSWLAMTRQPGLIGQRDDRDRPINTLAPDAERAGAPQARREVLAALSDVPPGTAPRADEVLELLAWQSPRRARGRESAHRDALTGAAALGVTGLGALTSYGKLLQVEAQIGDRDPLGAGPDGGRIPGASDAVRALDALLPAPVDHVLVQADLSVVVPGPPEPELAAELDVIAEPESAGGASVYRVTAASVRRALDVGYSAGDLHAVFQRRSRTPIPQALAYLIDDSARKHGGLRAGSAGSYLRSDDEALVAEVLADKRLSTLALRRLAPTVLCSPLQLARLLGALRDAGFAPVAEDAAGAAVLARPKVRRAAPRTSASARAGDQAGPPLLAGPRLAGVVEQIRRGDIATRAARRAPVTVRAANGQSVPGLTSVQAHTQAMAVLQQAVRDKARVWVGYVDSHGATLSRLVRPVSLSAGYLRAEDERTETLHTFALHRITAAVFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSW0</accession>
    <name>U5VSW0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02210</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02210"/>
    <dbReference type="EnsemblBacteria" id="AGZ38731">
        <property type="protein sequence ID" value="AGZ38731"/>
        <property type="gene ID" value="AFR_02210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.447"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_11_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="234"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="431" mass="44681" checksum="009D3BCAFF620F35" modified="2014-01-22" version="1">MEATEKVVEGKRKSWVIDVRPLRGVAYRRMWVGNGVAYFGFQFTSVAVPVEMFAITHSSAWVGLLGIAGLVPLLIFGLWGGAAADVVDRRKLLLASSTLAWIATVGLLVQAVLGLHSPIVLLALTAIQSAGFAISSPARQAIIPRIVPADLVPAANTLAYTTTTAGGVLGPLAAGLIFGAWSVDTGVTVAYAVDALLFTISFWATYRLPPIPPVLDDGVTEKPTAGLRGIADGLRFLVKQPVLLLSFAVDIIAMVLAMPRALFPEIAEDRFGGGSAVGWLFAAIAIGSVIGGLTSGWIGRIKRQGVALVIAVVGWGVAVGLAGLAHSLWLMVLLLAVGGAADLVSAVYRQSILQTFAPDRLRGRLQGVFTVVVAGGPRLGDLRAGATADITGPTASWAGGGFAAAGLAVVLALAFPALLRYRPPADAEVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSX6</accession>
    <name>U5VSX6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AMP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357773.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02705"/>
    <dbReference type="EnsemblBacteria" id="AGZ38830">
        <property type="protein sequence ID" value="AGZ38830"/>
        <property type="gene ID" value="AFR_02705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.544"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_11"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="110"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="443" mass="46915" checksum="89977DFEC67ED89C" modified="2014-01-22" version="1">MNLFHPEARVLDAATGEVLDRTRVDAAAAAFESRPPGVVFALMPTELDAVALYLGAFAAHRPIALLDPDLDHTVLLDLVERFAPALVTGVTTGPPDGYTAEDGWWVRDEPAVTPHPDLGLLLTTSGSTGNPKLVRLSRAAVLANAAAIGESLGITADDVAPTTLPLFYTFGLSVLNSHLLRGATVVLERTGILQRDFWQAVESYGVTSLAAVPYQYEMLRRLRFDPAKYPALRTLTQAGGRLRTALVEDFAQRMASVGGRLFVMYGQTEATARMAVLPPGHLPGKLGSAGLPIPGGSFSIADGEVVFTGPNVMLGYAESSADLARGDDLGGVLRTGDLGHLDDDGFLFLTGRSKRIGKVFGVRVNLDDVERTLAAHGAVAAVAGDDKLHVFVEGADSEAARSVRGELAGFLDTHFSGLDVRGIEALPLLPTGKIDYRSLEALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTF7</accession>
    <name>U5VTF7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357889.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03285"/>
    <dbReference type="EnsemblBacteria" id="AGZ38946">
        <property type="protein sequence ID" value="AGZ38946"/>
        <property type="gene ID" value="AFR_03285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.661"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_1_1_11"/>
    <dbReference type="OrthoDB" id="647535at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665187" evidence="1">
        <location>
            <begin position="20"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="2">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="2">
        <location>
            <begin position="134"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26276" checksum="A853C964ED1240F9" modified="2014-01-22" version="1">MRIALFVTCLADTMFPAAAKATVHLLERLGHEVVFPAGQTCCGQMHINTGYQKDALPLVRRHVQTFAPYDVVVAPSGSCVGSVRHQHAMVARAAGEEGLAARAEDVASRTYELSELLVDVLGVEDVGAYYPHRVTYHPTCHSLRMIRVGDKPQRLLRHVRGMELVELPAADQCCGFGGTFALKNADTSAAMLADKMRHVLDTGAEVCTAGDSSCLMHIGGGLSRLDAGVRTVHLAEILAATEETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUD2</accession>
    <name>U5VUD2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">rplM</name>
        <name evidence="5" type="ORF">AFR_04785</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358272.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04785"/>
    <dbReference type="EnsemblBacteria" id="AGZ39246">
        <property type="protein sequence ID" value="AGZ39246"/>
        <property type="gene ID" value="AFR_04785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.966"/>
    <dbReference type="eggNOG" id="COG0102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082184_2_2_11"/>
    <dbReference type="OrthoDB" id="1822215at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_L13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="147" mass="16447" checksum="FCF6498A3E0E219E" modified="2014-01-22" version="1">MRTYSPKPGEIERQWHIIDASDVVLGRLATHTATLLRGKHKPTFAPHVDTGDFVVIINAGKVALTGNKRQTKVAYRHSGYPGGLKQVRYEELLTKRPEHAVELAVKGMLPHNKLARQILKKLKVYPGAEHPHAAQQPVPFEIKQIAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUF5</accession>
    <name>U5VUF5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360338.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10815"/>
    <dbReference type="EnsemblBacteria" id="AGZ40452">
        <property type="protein sequence ID" value="AGZ40452"/>
        <property type="gene ID" value="AFR_10815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2204"/>
    <dbReference type="eggNOG" id="COG3795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130902_3_1_11"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="13153" checksum="B3399BAB45F42B56" modified="2014-01-22" version="1">MAEYLIYFNQQWVGDHTEEWFRGRGPLAKAVVDEIKAAGAFVFAGGLEEEDGPVFTADPTSGTIVVTDGPYVETKEFLGGLAIVNVPDEATARMWAGKIAEACGWPQEVRRFKPPRVLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV57</accession>
    <name>U5VV57_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360813.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40754">
        <property type="protein sequence ID" value="AGZ40754"/>
        <property type="gene ID" value="AFR_12340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2505"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079903_0_0_11"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="27043" checksum="657C0E0F64D27644" modified="2014-01-22" version="1">MAWSTRELADLAGTTVNTIRHYHRLGLLEEPTRRYNGYKQYGIEDFVRLLRIRRLVGLGVPLAQIQSISSDGDNGSQVLRQVDTSLAAEVERLRKVRSDIAAILRDGGSADTPSGFGAVAPRLSPSDSSLLHVITQLCDEDALADLKNMLESDPGPVDQEFDALPAEADEATRQRLAESLTPTLVRHLVDYPWLSDPAARLVKSEHVTRQTFVDAVIDLYNAAQIDVLVRAGLLAQKQLRLAHKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVF0</accession>
    <name>U5VVF0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_06535</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="EC" id="2.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359127.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06535"/>
    <dbReference type="REBASE" id="71862">
        <property type="entry name" value="M.Afr7358ORF6535P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39596">
        <property type="protein sequence ID" value="AGZ39596"/>
        <property type="gene ID" value="AFR_06535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1325"/>
    <dbReference type="eggNOG" id="COG0270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014695_1_0_11"/>
    <dbReference type="OrthoDB" id="1178320at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0680">Restriction system</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="532" mass="58413" checksum="DD6CBF8A63114AF2" modified="2014-01-22" version="1">MTLTVADYFCGAGGSSTGMHQVPGVRVVMASNHWQLAVDTHNANFPDADHDREDITRIDPRRHPSTDIGWFSPECTNWSQAKGMKVDYDGKPVQGNLFEQDDEAPLPDEAAVRSRMLMQDVPRFTEVHRYRAVIVENVTDVLKWSHLDQWLRRMRLLGYEHRIVVLNSAFATAYGPGAPQLRDRVYFVFWRTGGRSPDFAKWTRPRAWCPRCGDVRAVYVDKRPGVRRTMRYGQQYTYRCPATACRGRAVSPYALPAAAIIDWAVPAQRIGERVKPLEPKTLARIRAGLARYAGPSSLEAAGNTFERRPGVRTWPLVEPLRTVHTTASKALACPPLLVPVEGRDGKLARTVADPMRAQTARAENALVIPLRNNGVAKPADASPLLTVAAAGTHQGLLMRNNTGGAEMSTPVGEPMRALTTAGHQSLIGFDHLTYGYDTGRLRPLEMPLPAQTTVEGDALLGTAVDVDDCTFRMLTVDEIRAGMAFTPGYVLLGTAKRAKVRMLGNAVTPPAARDLVACVVEAITGDPFDLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVH2</accession>
    <name>U5VVH2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Betaine aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_06660</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359152.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06660"/>
    <dbReference type="EnsemblBacteria" id="AGZ39621">
        <property type="protein sequence ID" value="AGZ39621"/>
        <property type="gene ID" value="AFR_06660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1359"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_11"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07092">
        <property type="entry name" value="ALDH_ABALDH-YdcW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015657">
        <property type="entry name" value="Aminobutyraldehyde_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="17"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="478" mass="51347" checksum="55214E810D8BDAB3" modified="2014-01-22" version="1">MSTDLPVLRNFVAGSFVDTADGEVSPVVDPCTGETYAFAPVSSADDVAQAMTSAEAGFETWRDTTPAERQRALLRIADAVEARADEIIAAECRNTGKPVEATRAEEMAPLLDELRFFAGAARMLEGKSAGEYLRDHTSWVRREPIGVCAQVTPWNYPMVMAVWKFAPAIAAGNSVVLKPSDTTPVSTLLLAEIAAEFLPPGVLNVVCGDRDTGRALVVHPTPQLVSITGSTRAGMEVAVAAAADLKKVHLELGGKAPVVVFDDVDIEAAAKAIAGAGYFNAGQDCTAATRVLVDSRIAEDFTAALARAAQDTRVGGPGDPDAYFGPVNNAAQLGRVRGFLERAPEHAQVVTGGRQIGDRGYFVEPTVVAGLKQRDEMIQDEIFGPVITVQEFTDEDTAVRWANDVRFGLSASVWTRDHGRALRVSRRLNFGAVWINTHLPFVSEMPHGGYGHSGYGKDLSMYGFEEYTRIKHVMSHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW16</accession>
    <name>U5VW16_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Beta-glucosidase</fullName>
            <ecNumber evidence="2 6">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_14430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="2 6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361227.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14430"/>
    <dbReference type="EnsemblBacteria" id="AGZ41168">
        <property type="protein sequence ID" value="AGZ41168"/>
        <property type="gene ID" value="AFR_14430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2928"/>
    <dbReference type="eggNOG" id="COG2723">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001859_1_3_11"/>
    <dbReference type="OrthoDB" id="654705at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102483">
        <property type="term" value="F:scopolin beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017736">
        <property type="entry name" value="Glyco_hydro_1_beta-glucosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="Glyco_hydro_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="PTHR10353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03356">
        <property type="entry name" value="BGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="402"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="440" mass="48063" checksum="168566D58F0919DF" modified="2014-01-22" version="1">MAALPGFPSSFVWGVSTAAYQIEGAVGEDGRGRSIWDTFAHTPGRVRNGDTGDVACDHYHRFAEDVGLIAELGVDAYRFSIAWPRVQPDGSGSFNAKGLDFYDRLVDELGARGIAAMPTLFHWDLPQALEDGGGWLSRDTSYRFAEYAAAVVERLGDRVSRWITLNEPIVHMAEGYAFGTHAPGRELMFDALPVAHHQLLGHGLAVRALRAGGATEIMITNNCTPVIPASESEADLAAAAVYDGFHNRLFLDPVLLGGSPLTDGAPFVLDGDREIIATPLDALGINYYNPTRVAAAPEGSPLPFEMVPIEGVPTTAFGWPVVPDGLRGLLVGLLDRYADALPPIYITESGCSTVDEVTADGRIHDTARIDYLDGHLRALHQAIEEGVDVRGYFVWSLLDNFEWAEGYDQRFGLVRVDFDTQLRTRKDSFSWLRTALSDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWA3</accession>
    <name>U5VWA3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_14980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361337.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14980"/>
    <dbReference type="EnsemblBacteria" id="AGZ41278">
        <property type="protein sequence ID" value="AGZ41278"/>
        <property type="gene ID" value="AFR_14980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3042"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_302417_0_0_11"/>
    <dbReference type="OrthoDB" id="15684at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010496">
        <property type="entry name" value="3-keto-disaccharide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16990">
        <property type="entry name" value="CBM_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06439">
        <property type="entry name" value="DUF1080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666140" evidence="1">
        <location>
            <begin position="32"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="4">
        <location>
            <begin position="36"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="3">
        <location>
            <begin position="669"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="799"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1126" mass="119557" checksum="1C80C4C7720CF9FC" modified="2014-01-22" version="1">MKRSVPSRLPTPLLACAVLLAALLPATPAAAAAPPPQQPGVTLRTFDLQTSRDSICTLKPGQTPNIDKLMPTINWTTAADFGLEDRYQSEVIGNVNITTAGTYAFRLSSDDGSRLTIDSTRVINNDGAHGVVAVEGSIALTTGYHALHIDFFERDGGQQLTLEWRPPGATAYAVVPTSALSTDADVVRVTAPGRKECEGSADSPGDGLPLTSVHPGFTLTNLRPNGFQPQVTAMDWYPDGRLAIATWGGSDTVAGEVYILSNVTGSTSPGQVTTKRIATGLREPMGLKIVDGKVYVSQKHELTELTDTNGDDVLDQRRTVATWPFDGNFHEFAFGLLYKDGFFYLNLSVSINLGGATTEPQGSPNRGTSLKINRSTGAVQYVAGGLRTPDGIGWGPEGDIFVTDNQGGWLPSSKLLQIKQDRFFNHYTNPDGPYDSQPVTQPVLWLPQNEIANSPSTPLLLNSGPYSGQMLIGDVTYGGIQRAFLEKINGQYQGAVFRLTQGLEMGVHRLSQGPDGAIYAGGLGAGGNWGQEGKLTYGLQKLTPNSTSVFDIKAMRAVAGGFELEYTQPVSTDTAAQLAARYRAEQWRYVPTPAYGGPKVDEEQLGVTSATLSSDRLKVTLKLSGLKPGRVVHVRSPRPFTSTTGSSLWSTEAWYTLNSLADGTTVRTDQVEAENAGLSGRAGTASDHPGYTGTGFVAGIDTVGAATTFSVTVPSAGTYPATLRYSNGPNPSAGVKTMSLYVNGAKVRQVSLPSTANWDTWTTKVESVSLKAGGNTIAYKMDTGDIGNINLDNLAIGTSTGTATAPRTGTLTGIGGKCLDIDQANAANYAKVQLYTCNSSSAQSWTRDGDTLRALGKCLDIDNGGTANGTKVQLYTCNSGAAQVWQAQSNGSLLNPQSGKVLDVAGGATADGTQVQIYEYADVAQQKWTHNGTSRVTLFNGGNLQAFQNADGTAPTWPVSGGSAEVLGGDLRTKQTFGDFKLHAEFWLPNLPADVTGQARANSGIYLQDRYELQVLDTFGKATLADNDSAAIYLKRAPSANAGTAPETWQTYDVTFRAARWNGTTKTEDARVTVVWNGTTVHNNIAIDGPTGNGAAEGPNFLPIRLQDHGDPGANVRYRNLWIEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWG5</accession>
    <name>U5VWG5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15330"/>
    <dbReference type="EnsemblBacteria" id="AGZ41348">
        <property type="protein sequence ID" value="AGZ41348"/>
        <property type="gene ID" value="AFR_15330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3113"/>
    <dbReference type="HOGENOM" id="CLU_159290_0_0_11"/>
    <dbReference type="OrthoDB" id="1765599at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046179">
        <property type="entry name" value="DUF6188"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19686">
        <property type="entry name" value="DUF6188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="14610" checksum="CFF477E7AE2BC0F6" modified="2014-01-22" version="1">MVADMDAGRRPRQARSLDLLVGRKLEYVRLGHAVVLSFSGSCQVLIETLTHLDGPAGRVAIEPGENPSDILATLLGDVVREARALDHGELELVFRSGARLVVCVDADFESWAVAGPDGFLVVCLARGEIAVWGDGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWK7</accession>
    <name>U5VWK7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361313.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41254">
        <property type="protein sequence ID" value="AGZ41254"/>
        <property type="gene ID" value="AFR_14860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3018"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_2_0_11"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="6"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="24869" checksum="8C07BAC9918D57E3" modified="2014-01-22" version="1">MAKPTIVAVHGAWADASSWNAVTSELQERGYTVLAPPNLLRGLAADTGYLSSFVAQRTSGPVVLVGHSYGGFVITNAAAGGDVRALVYVNAFIPDEGETVFQILGGSGSAFDVPDPTTVFDIVGYPGGPEGDAEAFLKPETVHKSMAQDLSETDRLLVVAGQRPITLSANATPSGAAAWKILPSWAVVGTEDLVIPAATQRSMAARANAKTTEVAGSHVSMISNPQATITAILAAADHVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWZ8</accession>
    <name>U5VWZ8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase</fullName>
            <ecNumber evidence="4">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_09385</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359777.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09385"/>
    <dbReference type="EnsemblBacteria" id="AGZ40166">
        <property type="protein sequence ID" value="AGZ40166"/>
        <property type="gene ID" value="AFR_09385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1913"/>
    <dbReference type="eggNOG" id="COG1104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_0_0_11"/>
    <dbReference type="OrthoDB" id="839689at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="PTHR11601:SF34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="8">
        <location>
            <begin position="13"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ40166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="381" mass="39234" checksum="3A0372EC3D1BF864" modified="2014-01-22" version="1">MEYRAVHAGAPAYLDAASATPLHPVARQALLAALEDGWADPDRLYSAGRRAQQLREAAQATVAELLGVRPDELSFVSSGTAAVHAAVLGGLAGRVRVGRTLVHSAIEHSAVLHAAAQGEAVPVGVDLLGRLDLDAWNTAVAAPGVALASLISASHEVGTVQPVAAAAEACAEAGVPLYVDAAQSVGRSPVPPGWSLLTASAHKWGGPPGVGVLVVRKGVRWLSPYPGDDRHRPRPTGALDLPAVVSAAASLRAVLGEAQTEAVRLSALVEKIRATVAATVPDVEVVGDPVERLPHLVTFSCLYVDGEALLHALDRHGFAVSSGSSCTASTLQPSHVLEAMGVLSHGNVRVSLHRGTTEADVDRFLAVLPGLVADIRKEAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX01</accession>
    <name>U5VX01_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361458.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15585"/>
    <dbReference type="EnsemblBacteria" id="AGZ41399">
        <property type="protein sequence ID" value="AGZ41399"/>
        <property type="gene ID" value="AFR_15585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3165"/>
    <dbReference type="eggNOG" id="ENOG5031SQ7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167530_0_0_11"/>
    <dbReference type="OrthoDB" id="1987241at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="132" mass="14600" checksum="F7F41A5F525F5F98" modified="2014-01-22" version="1">MAAFAQVAPWFGDPLDFLGDVEDMERQSRALDRLVDDDEAARLFHLARPRLPADTAGLPWLDVEQAHFIAVSERVGDDTAIALDYRTDRADPSVVGSDIWTVPGQYHWRTISPTFSAFTAALGIDDPGRTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXD5</accession>
    <name>U5VXD5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-dependent Clp protease proteolytic subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_10505</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10505"/>
    <dbReference type="EnsemblBacteria" id="AGZ40390">
        <property type="protein sequence ID" value="AGZ40390"/>
        <property type="gene ID" value="AFR_10505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2141"/>
    <dbReference type="eggNOG" id="COG0740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318652_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21031" checksum="72EBA53AD04361C0" modified="2014-01-22" version="1">MTPNLRWQPNPPPQPDPWQGGGPQLPGWLEERLFDQRIVMIRGPLTGQAASGIAAALLTLDTAGPEPVQLHIASSGGDLNAAHAVIDVMDAMTAPVHAVVTSEAGGAVLAVLAAAERRSAYRHARFKLAEPRAAGVTGTADEVAAAAGQHLRELEEVVLRLVEVTGQSRSRIEDDLSAGRSLSSAEARDYGLIDEVVGKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXE2</accession>
    <name>U5VXE2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361521.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15900"/>
    <dbReference type="EnsemblBacteria" id="AGZ41462">
        <property type="protein sequence ID" value="AGZ41462"/>
        <property type="gene ID" value="AFR_15900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3228"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_11"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="321" mass="34953" checksum="4531FE04A2A49980" modified="2014-01-22" version="1">MTGVQIEGARALVTGGAGTIGSHVVDELIRGGAAEIVVLDNFVRGRRENLAWADANGSVRLVEGDIRDQALVREVTAGKDLVFHLAAIRITQCAEEPRLANEVLVDGTFNVIEAAAEEGVKKLVVSSSASVYGLAEEFPTTERHHPYNNDTFYGAAKAFNEATLRSFHSMKGLDYVALRYFNVYGPRMDIFGLYTEVLIRWMERIESGTPPLILGDGLQTMDFVHVADIARANILAARADVTDEVFNIASGTETSLKELAQALSEVMKSDLAPEHGPARAVNGVTRRLADTSAAAEKLGFRSEIDLHQGLQGLVEWWRSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXE6</accession>
    <name>U5VXE6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein-export membrane protein SecG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_10580</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Involved in protein export. Participates in an early event of protein translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SecG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360249.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10580"/>
    <dbReference type="EnsemblBacteria" id="AGZ40405">
        <property type="protein sequence ID" value="AGZ40405"/>
        <property type="gene ID" value="AFR_10580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2157"/>
    <dbReference type="eggNOG" id="COG1314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094156_7_1_11"/>
    <dbReference type="OrthoDB" id="2057754at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004692">
        <property type="entry name" value="SecG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03840">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01651">
        <property type="entry name" value="SECGEXPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00810">
        <property type="entry name" value="secG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ40405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="84" mass="8752" checksum="ACA830B7B65CFA80" modified="2014-01-22" version="1">MPIAFAYTLIVLLIISSILLTLLVLLHRGKGGGMSSMFGGGVTSSLAGSSVAEKNLDRYTVLVGVIWFACIVGLGLWLKLAQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXE7</accession>
    <name>U5VXE7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein tolB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361526.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15925"/>
    <dbReference type="EnsemblBacteria" id="AGZ41467">
        <property type="protein sequence ID" value="AGZ41467"/>
        <property type="gene ID" value="AFR_15925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3233"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070328_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="349" mass="36906" checksum="0BA437CAFAB2778C" modified="2014-01-22" version="1">MTSTDLRPAGTVKMRAGLLAGIVVAAAAGAGGYVWNVRQDQAATIANAPAVAAGGDLTTLRTQPHLVFRSTALGPDYGRVAVVPLTAPGGPRTFTPATCERVYATQGDAICLSAQRGLVTSYQARLLDASWAPTRDLPLTGVPSRARLSRDGSLAATTTFVFGDSYANPGQFSTRTIVTRTDGEVVGDIEKFKLTVDGKVVKAADRNFWGVTFVDDDRFYATAASGGKTWLVEGSLKSRAVTALRGDVECPSLSPDGTRIAFKKHGDLPAGRWRLAVYDLRSGTETLLAETRSIDDQVEWLDDSTVVYGLPRDTASSASSDVWKVPADGSGAPQTLIQDAWSPAVVRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYK8</accession>
    <name>U5VYK8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_12595</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12595"/>
    <dbReference type="EnsemblBacteria" id="AGZ40805">
        <property type="protein sequence ID" value="AGZ40805"/>
        <property type="gene ID" value="AFR_12595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2558"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_483684_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="251"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="354"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="542"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="585"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="48"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="418"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="624" mass="67076" checksum="1F7295DC232F631F" modified="2014-01-22" version="1">MLVSVLVLVPIVQSIITSTTERHGQDNVFVGLGNYVALAGDEQFRTGVVNSFVFTAYAEIFKVVLGLAAALLLHHRRRGRALLTGLLLVPWVVPTVVTAFSWRALLDPIFGSVNTLLTSSGIGPLLADLHLVDSWPAGWLSDPSLAMPSVILVNVWKGVPFFAVCFLAGLKAIPADQYEAATIDGASAWQRFSHVTLPGLRHVITVTVTLSSIWTFNNFDLIWLLTQGGPGNVTTPYVLVAYTKAILQLQYGAGAAVTLVMLPVIGALVFVLVRLLRQEGGAAPARTARWAATTRRALPWVLTVVVTVLLAWASPQIFWKAAVVLAVFALIAAAVARVVSALQSRGGRRASSLVAGLGSGVALAGLLAFVLGPLYWIVVTAFKSEDQVVMRTDDLWPTPWTLEQFGALFANQPFGRWYLNTILVSAASTAVALICAALAGYALARLRFRGAQGFTVTVLLTYVMPGALLFIPLYQLLIGVRLTDSLWSLVLTYPTFTIPFATWLLTGYFASIPIDLEEAALVDGSTRIQAFRRVVLPLAKPGLLAVALFTLTNAWNEFLLAFVFITKDEYKTLPVGMQSMIAGDVVPQGQLAAASLLVSIPVVIMYAFGQRFLTEGLTAGAVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYP8</accession>
    <name>U5VYP8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360909.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12820"/>
    <dbReference type="EnsemblBacteria" id="AGZ40850">
        <property type="protein sequence ID" value="AGZ40850"/>
        <property type="gene ID" value="AFR_12820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2604"/>
    <dbReference type="eggNOG" id="COG2867">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127145_0_0_11"/>
    <dbReference type="OrthoDB" id="1597462at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="150" mass="16102" checksum="58612ACA8BC7AC01" modified="2014-01-22" version="1">MPTIHLTRTIRVTPEQFVAAVTDFGPGRSKIFGNTSDEVLEVHGLGETTADVTEGTKAGWERLHYDWLNPHRVTMTTVDSNLWGGSSGHTYELVPQGDGTTRLDVTIVREGKNVAGRLAGLLVGSIAKGALGKAVSNTVTAIEQRYGVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYS6</accession>
    <name>U5VYS6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amine oxidase</fullName>
            <ecNumber evidence="3">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_19155</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="3">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="3">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="2">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2 3">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362476.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19155"/>
    <dbReference type="EnsemblBacteria" id="AGZ42103">
        <property type="protein sequence ID" value="AGZ42103"/>
        <property type="gene ID" value="AFR_19155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3890"/>
    <dbReference type="eggNOG" id="COG3733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011500_5_5_11"/>
    <dbReference type="OrthoDB" id="611729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary amine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="PTHR10638"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="SSF49998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3" id="KW-0801">TPQ</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665883" description="Amine oxidase" evidence="4">
        <location>
            <begin position="28"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxid" evidence="5">
        <location>
            <begin position="51"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="1">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="2">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="421" mass="45920" checksum="75669CD544974412" modified="2014-01-22" version="1">MRTTKIMAAVLIATGGLIGTAQSPAAAKAPAPAAAQCAASSTVKETLPNGTTWELCWRIEGKSGLVLENVLVSSKRWPDQVKVLKSVTLAQLNVPYDSGKTEYNDITEYGFGGEYLQTLAGDDCKGGSARVGSDGGAEPTRREVLCVSAESSGLAYRLQNDDSATKPYSKQGHDLVLRALSKVGWYEYVTEYRLHDDGEISARLGATGDLSPLDWVGADKGWPIGKGNKDFASSHYHSAFWRVDFDLAGRGSEKVEQYDTKVTGQGDTSAVLKTSRTAITNEGSFSKANRRWWRVVSPIAKNKDGHARSYELVTGASDVYEAHPETTPDVTFTQNHACERYATGNADPQCAGRATILDFARDKEKMTNPVMWVRVGFHHVPRDEDQSPMPLHWQGFDLVPRDFTEINRLAPDALADNNGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYY0</accession>
    <name>U5VYY0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_13270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360999.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13270"/>
    <dbReference type="EnsemblBacteria" id="AGZ40940">
        <property type="protein sequence ID" value="AGZ40940"/>
        <property type="gene ID" value="AFR_13270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2694"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_2_11"/>
    <dbReference type="OrthoDB" id="1992113at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="12"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="107"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="20319" checksum="1891A5B06D8565F8" modified="2014-01-22" version="1">MNREAEDTFRDLFQAVYDDLLCFVERRSGPAVAEDVVSETFLVAWRRLHDVPDSLDGARAWLFTVAHNLLRNRQRSEQRQQNVALRILRQPEGPAAGDADAVAAHVDLTRAWQRLSPADQEALALTAIEDLTGPQAAHVLGITRTAFSLRLLRARRNLRRHLRHQPAPSPAISRTASHGGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ87</accession>
    <name>U5VZ87_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HTH-type transcriptional repressor purR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18940"/>
    <dbReference type="EnsemblBacteria" id="AGZ42062">
        <property type="protein sequence ID" value="AGZ42062"/>
        <property type="gene ID" value="AFR_18940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3847"/>
    <dbReference type="eggNOG" id="COG1879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665896" evidence="1">
        <location>
            <begin position="28"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_4" evidence="2">
        <location>
            <begin position="43"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="328" mass="34687" checksum="589554BEE2C6B52F" modified="2014-01-22" version="1">MLKKWSAAVLAGALALGALAGCGGDDAGGGTGSGGDDKLTLGFSQVGAESGWRTANTKSIQEAAATAGIELKFSDAQGKQENQIKAIRSFIAQRVDVIAFSPVVESGWDTVLKEAKDAEIPVILTDRAVDSKDTTLYKSFIGSDFVLEGKKAGEWLVKDYEGKNDPVNIVELQGTPGAAPANDRKAGFADVIKADPKFKVVASQTGQFTRTDGKEVMEAFLKSQPKIDVLYAHNDDMGLGAIEAIEAAGKKPGTDIKIITVDAVKDGMTALSEGKINYIVECSPLLGPQLMDLAKKVKAGETVEARVLTEETTFTQEQAKAVLAERKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZS6</accession>
    <name>U5VZS6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bcr/CflA family efflux transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_14345</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Bcr/CmlA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14345"/>
    <dbReference type="EnsemblBacteria" id="AGZ41151">
        <property type="protein sequence ID" value="AGZ41151"/>
        <property type="gene ID" value="AFR_14345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2911"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_47_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004812">
        <property type="entry name" value="Efflux_drug-R_Bcr/CmlA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00710">
        <property type="entry name" value="efflux_Bcr_CflA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="21"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ41151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="406" mass="41209" checksum="3F38E890CEEEDDE2" modified="2014-01-22" version="1">MVTLTVDSPQNLDRARLTSGLVATVIFLTAIAPLATDMYVPAFPRVAGDLAGTATQVQLTLTTFFVGMALGQLVGGPVSDQRGRRRPLLAALVLMAAASVVCALTPSIGVLMAARFVQGFTGGWAMVIGRAVIVDLATGARLIRVLNVVAGVGGIAPIAGPLLGAVILQLSHWRVSFWVVAALGLVMVVAVLVAVPESLPPERRHGGGLRAFAVAGRLVLANRRYVGYLLVSGSAMGALFAYVATSAFVLQSMNGLSPMMYSADFAANAAGMTVAALVAARLAGRVATRKVILTGQVAALAAGVAMLAGAIWFGTPLLLAVVCFLVLMTAQGLIGPNGGALASAEVPEHPGTGSAILGFVQWVAAGTIAPIAGLGGEHTAVPMALLMIAGAVLSMIGLLVLARPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZT4</accession>
    <name>U5VZT4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362870.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21130"/>
    <dbReference type="EnsemblBacteria" id="AGZ42498">
        <property type="protein sequence ID" value="AGZ42498"/>
        <property type="gene ID" value="AFR_21130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21130"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165504_0_0_11"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="2">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="99" mass="10196" checksum="2E6D3E4B250F953C" modified="2014-01-22" version="1">MSQPGVVREFHAGEGWGVLDGPGVPGGCWVHFSAIAGPGFRSLAAGQAVTFRAVAPGQDGYPYRAEKVWTTAGEPDDQIADGPSDAYGSSLTLSFDDPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W096</accession>
    <name>U5W096_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative xylose repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15320"/>
    <dbReference type="EnsemblBacteria" id="AGZ41346">
        <property type="protein sequence ID" value="AGZ41346"/>
        <property type="gene ID" value="AFR_15320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3111"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="374" mass="39068" checksum="B60607DB8BB6A433" modified="2014-01-22" version="1">MDVIRSATTISRVELAELTGLTQPSISNIVRDLIADGVIHETGSADSIRGKPRKLLAINAANRFGIGFQVGAESLTCVAIDLTGGVVGREVVPLTTADLPAQLAERFDDFTDGLDLPRDRVEGLAIVAPTARPGHESAAPEWGVVRADLADHLKIPVLVENDAAAAALGEFWTRRVSREQSFGSVYLSTGIGAGLVFGGALFRGASFDAGELGHSSIRYDGRPCPCGNQGCVERYASMAATVDEARADPALTSRLRLDGTVSSAYDAIARAAVRGDADAFALVDRAAGYLSVAVTSMVNLLDLGRLVLTGPGVAVAGSIYARRLRAHLARTAHARRRHEIVVELSAQPRDAASIGAAALVVQASIAPGHTLGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0C5</accession>
    <name>U5W0C5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquinone/menaquinone biosynthesis methyltransferase ubiE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361499.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15790"/>
    <dbReference type="EnsemblBacteria" id="AGZ41440">
        <property type="protein sequence ID" value="AGZ41440"/>
        <property type="gene ID" value="AFR_15790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3205"/>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056435_4_3_11"/>
    <dbReference type="OrthoDB" id="2049098at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="37"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="20791" checksum="61290FB77DBAF351" modified="2014-01-22" version="1">MSDYDAIYRAGPPPWDIGVPQPAIAAVLDDRVRGPKVLDIGCGTGTLAIDLARRGYEVTAVDISGVAIDEARSRAAAAGVTVHFGVQDATALDLPSAPFDSVFDSGLLHSLNRHGGSEVDDYLAQLPDLAAPGATVFVLAVSLEPGQVWGVTEEFLRASFAEPVWTGTAIEKIEISAVADGEQLRLPGFLLRSVRNPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W111</accession>
    <name>U5W111_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Purine nucleosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_22160</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363073.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22160"/>
    <dbReference type="EnsemblBacteria" id="AGZ42702">
        <property type="protein sequence ID" value="AGZ42702"/>
        <property type="gene ID" value="AFR_22160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4492"/>
    <dbReference type="eggNOG" id="COG1957">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036838_2_1_11"/>
    <dbReference type="OrthoDB" id="1763387at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901564">
        <property type="term" value="P:organonitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="PTHR12304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="SSF53590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IU_nuc_hydro" evidence="3">
        <location>
            <begin position="6"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="31067" checksum="E242A5473DC5313C" modified="2014-01-22" version="1">MTAFHLDCDTGIDDALALAYLLGHPGVDLTGIGTVSGNTAAGQAARNTLDLLALAGRDDVPVATGAHHPLGGDFAGGAGPVHGSNGIGGVELPHTTRMPDDRSATDLLIEAAHRHPGELRVITTGPLTNLALALRREPELVTLVHDVTAMGGAVRAPGNITGNAEANIANDPAAAAEVLGAGWPVTLVPLDVTMQHRFTEHDRAALAGHGTPLTAALAAMLTSYFDFYETRFGERQVPLHDPLAAGIATGDLVPLDAPLLGLRIESDGQVVEDTAARHPTKVVFSLTGAAAPVIRTLILEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W182</accession>
    <name>U5W182_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363177.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22510"/>
    <dbReference type="EnsemblBacteria" id="AGZ42772">
        <property type="protein sequence ID" value="AGZ42772"/>
        <property type="gene ID" value="AFR_22510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4563"/>
    <dbReference type="eggNOG" id="COG0645">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105030_2_0_11"/>
    <dbReference type="OrthoDB" id="1574819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="178" mass="19892" checksum="E8E373D603AAA417" modified="2014-01-22" version="1">MSQNHAQLFLMVGLPGAGKTTRAKEFAALRLTPDEWMVQLFGEPTAGRKRDVLEGRLILVALEALRLGTSVVLDFGLWGRDERSALRRLAASAGASCQVIYLPVGREVQRERIAHRQATAPHTTFPMSEDDIDSWRKQFQEPDTAELLGVDRPEPPPGWTGWPEWAADRWPPLVVLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1I8</accession>
    <name>U5W1I8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2,2-dialkylglycine decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363604.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23730"/>
    <dbReference type="EnsemblBacteria" id="AGZ43014">
        <property type="protein sequence ID" value="AGZ43014"/>
        <property type="gene ID" value="AFR_23730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4806"/>
    <dbReference type="eggNOG" id="COG0160">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_11"/>
    <dbReference type="OrthoDB" id="1322538at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="443" mass="47606" checksum="8EF2FE8E4D65B3A2" modified="2014-01-22" version="1">MDATFWNDAERHLVRYSGAGSFVPEIIERAEGSFVWTADGRRVLDFTSGQMSAILGHSHPAVVATVQEQVAKLDHLFSGMLSRPVVDLARRLAETLPEPLSKVLLLTTGAESNEAAIRMAKLVTGRHEIVSFGRSWHGMTQAAASATYSSGRKGYGPAAPGNFAIPTPNAYRPDFTTADGDLDWRRQLDFSFELFDAQSVGSLAACIVEPILSSGGVIDLPPGYLAALRDKCHERGGLLILDEAQTGLCRTGDWYAFQRDGVVPDLLTLSKTLGAGLPLAAVITSPEIEQTAHERGFLFFTTHVSDPMVAAVGNTVLDILYADKLDQRAAELGAYLRKGLLELAGRHPVIGDVRGRGLLVGLELVLDRTTKQPADDLGAAVTRRCLELGLHMNVVQLPAMGGVFRIAPALTSTPEELDLGLEILDQAITDTAPRFTAISAGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1K5</accession>
    <name>U5W1K5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5-oxoprolinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362065.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17590"/>
    <dbReference type="EnsemblBacteria" id="AGZ41796">
        <property type="protein sequence ID" value="AGZ41796"/>
        <property type="gene ID" value="AFR_17590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3565"/>
    <dbReference type="eggNOG" id="COG0146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020413_1_1_11"/>
    <dbReference type="OrthoDB" id="1018930at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="PTHR11365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase_B" evidence="1">
        <location>
            <begin position="18"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="623" mass="68218" checksum="5BF1EDE2ACBB90B7" modified="2014-01-22" version="1">MARIVERDTTPLARADVDPVTLDLVENGLRNARYEMDEVLFRTALSPGIREQHDEFPLIADASGKMVVGQFGLSIPDFLAGFDGDIGEGDVLLTSDPYACGAAISHANDWLVVLPIYVDGRVVGWASMFGHMSDVGGKTPSSMPTDARTIYEEGVVIPPFKLYENGKVNEDALRIILNQVRVPDWNRADLNGLVAACRTAARRVQEMCARFGTATYLSALDDLLQRNHDAMKVLLQVVFEEGRTLSFTDYICDDGVGFGPYELKLSLTRTGEKVHLDFTGSSPQAAGPINYYLNENLARMFFGIYMITVADPQILWNDGFYPLVDVTIPDGSYWKPKHPAALNARNHGIGRVFDLFGGLLGQTNPALLNAAGFSSSPHFMYSGNYSGGDRKGEWFQLYSIGFGGIPGRPLGDGPDGHSLWPSFVNIPCEFLESYYPLRIEKWETLADTGGAGLHRGGNGVDVAYRFLEPGTIAIHDDRWLMYPWGVNGGTPGARGRKWIDRAGGEREILPSKCHDVPVHPGDVLHFVTWGGGGWGDPLERDPALVGLEVRRGLVSSDGARRYGVVTDDGGAVDTDGTEDLRRRLRAGRPDELPVFDMGPGLDEILARCEEETGLPAPTRPVWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1N1</accession>
    <name>U5W1N1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17740"/>
    <dbReference type="EnsemblBacteria" id="AGZ41826">
        <property type="protein sequence ID" value="AGZ41826"/>
        <property type="gene ID" value="AFR_17740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3597"/>
    <dbReference type="eggNOG" id="COG2258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104911_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="25"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="15731" checksum="50AA70108A5198B0" modified="2014-01-22" version="1">MQLLASPVHRFEGRPGDGPLPAPEGELAGSIRIRAGLGIVGDRYFGKPAHRDASVTVIAAESLPSGAGLVQVRRNILVRGIAVDTLIGAVLTLDSGDGPVSLQVRRRANPCAWMDVTLGPGGFKALRGKGGIRCVPLNDGVLRVGPVEISVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1W5</accession>
    <name>U5W1W5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">4-hydroxy-2-oxovalerate aldolase</fullName>
            <shortName evidence="7">HOA</shortName>
            <ecNumber evidence="7">4.1.3.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">4-hydroxy-2-keto-pentanoic acid aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">4-hydroxy-2-oxopentanoate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_23745</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(S)-4-hydroxy-2-oxohexanoate = propanal + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:36003"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:17153"/>
            <dbReference type="ChEBI" id="CHEBI:73142"/>
            <dbReference type="EC" id="4.1.3.43"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:36004"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:22624"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:73143"/>
            <dbReference type="EC" id="4.1.3.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 4-hydroxy-2-oxovalerate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.39" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363607.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23745"/>
    <dbReference type="EnsemblBacteria" id="AGZ43017">
        <property type="protein sequence ID" value="AGZ43017"/>
        <property type="gene ID" value="AFR_23745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4809"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049173_0_0_11"/>
    <dbReference type="OrthoDB" id="840579at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008701">
        <property type="term" value="F:4-hydroxy-2-oxovalerate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07943">
        <property type="entry name" value="DRE_TIM_HOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01656">
        <property type="entry name" value="HOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017629">
        <property type="entry name" value="4OH_2_O-val_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012425">
        <property type="entry name" value="DmpG_comm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035685">
        <property type="entry name" value="DRE_TIM_HOA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07836">
        <property type="entry name" value="DmpG_comm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03217">
        <property type="entry name" value="4OH_2_O_val_ald"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="8">
        <location>
            <begin position="5"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="13"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="35598" checksum="1B077B5D4017D255" modified="2014-01-22" version="1">MSTRLFVQDVTLRDGMHAVRHRISPADVARIAAALDAAGVDAIEVAHGDGLAGGSLTYGPGSNTDWEWIEAAAGAVTRARLTTLLLPGIGTIAELRQAYRLGVRSVRVATHCTEADVAAQHIATARDLGMDVSGFLMMSHLAPPDVLAAQAELMESYGAHCVYVTDSGGRLTMTGVRERVRAYREILEPGTEIGIHAHENLSLSVANSVVAVEEGAVRVDASLAGHGAGAGNCPIEPFIAVADLQGWEHGCDLFALQDAADDLVRPLQDRPVRVDRETLTLGYAGVYSSFLRHAEAASSRYGVDVRRILLEAGHRHLVGGQEDMLTDIALDLAATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1W9</accession>
    <name>U5W1W9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_18215</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362294.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18215"/>
    <dbReference type="EnsemblBacteria" id="AGZ41921">
        <property type="protein sequence ID" value="AGZ41921"/>
        <property type="gene ID" value="AFR_18215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3697"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_11"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="362" mass="39116" checksum="2D8510EDDC1DDCE5" modified="2014-01-22" version="1">MAGVVVSNLVKTYPSGNRRATDDVTLEVADGEFLVLVGPSGCGKTTLLRMVAGLETPDSGTVTIGGRDVTGLPARERGLSMVFQSYAIFPHLKVRQNIGFGLTMRKVGSQEWESRVQKAAELLDLTDYLDRYPAQLSGGQRQRVAVARAIVVDADVLLMDEPLSNLDALLRMQFRAELKKIVARLGTTTLYVTHDQAEAMSLGDRVAVMRDGRIAQLGTPMDVYDRPGEKFVGSFLGAPPMNFFTAAVRQEGSGVRLRVGDEELAGPDRLARWSGRDVLVGLRAENIEVSATAEEGWQSAEVEVAEPTGAAVLLTVRWGPHELKVQAGPGFAAKPGDRVWLRGPVTALRFYDPETSLALPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1X9</accession>
    <name>U5W1X9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362304.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18265"/>
    <dbReference type="EnsemblBacteria" id="AGZ41931">
        <property type="protein sequence ID" value="AGZ41931"/>
        <property type="gene ID" value="AFR_18265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3707"/>
    <dbReference type="HOGENOM" id="CLU_962558_0_0_11"/>
    <dbReference type="OrthoDB" id="1587707at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="29868" checksum="62CC3735F3CF4608" modified="2014-01-22" version="1">MKIEIEATADLWFGPGLPVRAASAVAPFGDGWLVVQDDATHAAWLRPGGIEAVRVADAVDGHETFSAADGTKHLKPDFEAACPVPGGVLLLGSGSTGARMRASLVTPDPAVTTTDLTAVYRRVAEVLGLRPDRLNLEGACLVGDRLRWFQRGGSAAVDLDFPGLLDVLTGRAEPDAVTVRAAGHWDLGSVAGVPLSITDAVALPDGRIVVSAAAEDTPNPVDDGPVVGSALALLDDTGVLAIAPVPDGPYKIEGLAVRQADPAGVSLIAVVDADDPLLPSTRLDLRLHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W228</accession>
    <name>U5W228_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PG_binding_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25085"/>
    <dbReference type="EnsemblBacteria" id="AGZ43283">
        <property type="protein sequence ID" value="AGZ43283"/>
        <property type="gene ID" value="AFR_25085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5083"/>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2393257_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PG_binding_1" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="92" mass="9795" checksum="37DB10F9F38ECE49" modified="2014-01-22" version="1">MLGVVGTAVGGLIGWTDGQAPAADPASVRQLFADLGLVVGGTEDELDSAVRRFQTRVGLRQDGVAGPRTVHLLARYAKEARELRRFEYLSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W297</accession>
    <name>U5W297_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Drug resistance efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_24940</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24940"/>
    <dbReference type="EnsemblBacteria" id="AGZ43254">
        <property type="protein sequence ID" value="AGZ43254"/>
        <property type="gene ID" value="AFR_24940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5052"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="523" mass="53825" checksum="87ED1D85BD020A89" modified="2014-01-22" version="1">MVAIALGVSMIVVDTTIVNVITPSVIDDLGISSSQAQWVQESYAIMFAALLLLFGRVADLVGARRVFVLGAVVFGVTSLLAGIASSGNVLILARFLQGAAGAMLLPTSLSLLNQTFTGKARGQAYAVWGSTIGAATALGPVLGGWLSEHASWRWAFGINIPLVVVIVALSLVFIPMSKRTAGRVDVPGALLSFAGLAALAFGLIEGRVYGWVTSRSPFTVLGQTWDGGLSPVFVALVIAAALLALFVRRQVSLSRNGSGGEPLMNTRLFRISSFRNGNIATLIIGLGEFGIIAVIPLWLQFTLGYTSLEAGFALVPLALGSFLASGLSFGLKLSALALVRIGLVLEAVTLAALGVVAATTSAPWWSVALVLFGYGIGVGFATAQVTNVVLADVPDDQGGQGSGIQSTFRQLGSALGIAAITTIFFSALDNGVRTNLRAEDLPGDQIDRFAAAVTDSAGAVIAPLAGDPVTAVVARAARDAMTHGVELGSYVAAGFLVLGVLATLLIPAPRTAVTAEIPEELPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2A7</accession>
    <name>U5W2A7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">L-ribulose-5-phosphate 4-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.4" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25545"/>
    <dbReference type="EnsemblBacteria" id="AGZ43373">
        <property type="protein sequence ID" value="AGZ43373"/>
        <property type="gene ID" value="AFR_25545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5176"/>
    <dbReference type="eggNOG" id="COG0235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008742">
        <property type="term" value="F:L-ribulose-phosphate 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="1">
        <location>
            <begin position="12"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="24015" checksum="2C16E55A0B5FFC4C" modified="2014-01-22" version="1">MIITDAIKQIRQQVADLHAELPRNELVVWTAGNVSARVPGADLLVIKPSGVRYDEITADNMVVTDLEGTLVDGELSPSSDTAAHAYVYKHMPHVGGVVHTHSTYATAWSARGEAIPCVLTMIADEFGGEIPVGPFALIGDDSIGRGIVETLQGHRSPAVLMRNHGVFTIGASAKAAVKAAVLCEDVARTVHISRQLGEPVRIAQADVDALYDRYQHAYGQESTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2B2</accession>
    <name>U5W2B2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ankyrin repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363742.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24430"/>
    <dbReference type="EnsemblBacteria" id="AGZ43152">
        <property type="protein sequence ID" value="AGZ43152"/>
        <property type="gene ID" value="AFR_24430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4949"/>
    <dbReference type="eggNOG" id="COG0666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_992599_0_0_11"/>
    <dbReference type="OrthoDB" id="1360387at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="29824" checksum="FD13283DBDA05655" modified="2014-01-22" version="1">MTIRDALSATKFHPAPAALGLLRDLLRAGLAEEPGDEPVLIRAVGSTVPPAALRALLEAGADPDQKRSDGTPALVLAARRGDHAAVDVLLEAGADVDGADGIGRTALMHAVERDEQRVVTVLLLAGADTEVVSHDGMTALRLARGWRRQAIRSRLGERFIGRAKVPIIRTAIGLEPTGVRLTADAESFRLLATVLEVALDHLGDADWRTRTGWDAVTARGFAARLRAVSLEELEELEATEEEVGTVRAALVEVSHGFTVPPATGLDLTRVADLLEDFKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2H0</accession>
    <name>U5W2H0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362474.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19145"/>
    <dbReference type="EnsemblBacteria" id="AGZ42101">
        <property type="protein sequence ID" value="AGZ42101"/>
        <property type="gene ID" value="AFR_19145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3888"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_11"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="20"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="360" mass="38859" checksum="36926BC7CED5A7A5" modified="2014-01-22" version="1">MSLDSYVSLGRSGLRISPFTLGGMTFGEDNGWGCSPAEAEAIMTEYLDRGGNSIDTANIYTNGHSEKIIGDFLAARPGLRDRIVLGTKFFGNLFPGDPNGGGAGRKAIVHQLEESLRRLQTDFVDVYWVHNWDRTAPIEETLRALDDLVTAGKIRYVGLSDLPAWKVAEAQVTAFFRGWAPAIGLQVEYSLLERTVEGELIPVARDKGLGVLPWSPLRSGFLSGKFSRRRAGAVDTLRSGLVGVPGEHDYDVIDVLERVADEVGATPAAVALAWVQGRPGVTSTLLGARRLDQLKNNLDAVDVRLEAEHVAALDEVSAPKLNFPAENNSLLAPNLAFAGATVDGQRTTLLPLFGTDGTRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2M6</accession>
    <name>U5W2M6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">YwfM-like transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_25600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364034.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25600"/>
    <dbReference type="EnsemblBacteria" id="AGZ43384">
        <property type="protein sequence ID" value="AGZ43384"/>
        <property type="gene ID" value="AFR_25600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5189"/>
    <dbReference type="eggNOG" id="COG5006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057295_0_2_11"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666015" evidence="2">
        <location>
            <begin position="32"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="147"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="29679" checksum="7AF23CEDF6AAB627" modified="2014-01-22" version="1">MTTVVQRRRAVPAPLLVLTAIASVQVGSAVARGFFDELGAAGITLLRLLIAAVILGVVFRPKVRAWSAAAWRAAALLGVAMAGMNLLFYLSLRTVPLGVAVTVEFLGPLLLTLGQTRRVADLMWALLAGGGVALLGLDPTVTAPLGGLLLAFAAGLCWAGYILASAHLGTLVPGTGGLAVALAGAALLVLPFGASGATAVLDHPSLLLGATAVALLSSVFSYGLEINALRRIPTRVFGILMSLEPAGAAIAGLLVLHQRLGLREVAALLLVSLASLGATLAHRDPVTPPVAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W306</accession>
    <name>U5W306_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364415.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26580"/>
    <dbReference type="EnsemblBacteria" id="AGZ43578">
        <property type="protein sequence ID" value="AGZ43578"/>
        <property type="gene ID" value="AFR_26580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5387"/>
    <dbReference type="eggNOG" id="COG1426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055817_1_1_11"/>
    <dbReference type="OrthoDB" id="1801508at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043917">
        <property type="entry name" value="DUF5753"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19054">
        <property type="entry name" value="DUF5753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="33531" checksum="0E73396FBD05AE7F" modified="2014-01-22" version="1">MTAARDETPAVARQRVRRALRKARRAAALTQSDVSTALRWSLSKVQRIESGEVGISVTDLRALLDVYSVVDEALIEALIHDAVISRRQRWLVTSEHREHLTPGLRQLLQFEAQATVIRAYQPFIFPGVLQTPAMAEHLLGWFDKSLSEQDRKVRFDVRMLRARRITQQEDAPTYLLMLDEAALRREIGGKAVMADQLEAAAAAAARTNIHIRIVRLAEGATVGMLGAFTVLDLSDDEDDVVLYREFYSTDQVIHDAETVSSFRARFESMWSRCYSRQDSLGLIRAEAASLRASLIRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3E0</accession>
    <name>U5W3E0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_20895</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20895"/>
    <dbReference type="EnsemblBacteria" id="AGZ42451">
        <property type="protein sequence ID" value="AGZ42451"/>
        <property type="gene ID" value="AFR_20895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20895"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4236"/>
    <dbReference type="eggNOG" id="COG2409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005108_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666050" evidence="7">
        <location>
            <begin position="26"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="552"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="591"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="183"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="517"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="668" mass="68547" checksum="6E6104D625A1848C" modified="2014-01-22" version="1">MTGRFTAWLVLAIALLAGGSVIAGAGEVRTANDPTASLPAAAESTKVAALERQLPSGQTNPALVVYSRGSGALSDADVEKIEADAQAFRAAGPPVFSPQRDAALVAVPLPADAPAQQLIDAVAGLRSTAQAGLPEGLTAQVTGGAGFAADIADSFTGANTNLLIVTVVVVALLLLVTYRSPILWLVPLAVVGVADRVGSGLVALLSRHTDLTIGDSTVGIVTVLVFGAGTDYALLLISRYREELHQHEDRREAMRRALRGAGPAVAASAVTVVLSLLTLLLASLGDTVALGVTAALGIGVAALFALIALPAALVVCGRGLFWPFIPRVGEGGAEQERGIWARVGRGVARRPGLVTVVSLVVLGVLSTGLVGTRLGLSQTEQFRVQAESVDGLETLSRSFPAGAADPAVVVTAPARAQQVLAAVTATPGVAQARIAEETPETTRIDAVLTAAPDTADSYETIRALRDKVGDDALVGGSVAIALDSRDAARRDLRVIVPVILLVVLLVLGLLLRAVTAAVLLIVTVIASFTAALGAGSWLFREVLGYPGLDNQVPLYAFLFLVALGVDYNIFLVTRAREEAAIRGTRAGMIHALAATGAVITSAGILLAAVFAVLGVLPIITLTQIGVIVGLGVLLDTLLVRTVLVPALATLLGDRFWWPANPAANRAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3H1</accession>
    <name>U5W3H1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase, PA-phosphatase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560007.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27040"/>
    <dbReference type="EnsemblBacteria" id="AGZ43669">
        <property type="protein sequence ID" value="AGZ43669"/>
        <property type="gene ID" value="AFR_27040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5480"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061746_0_0_11"/>
    <dbReference type="OrthoDB" id="1837609at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="104"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="32247" checksum="94CE399D7333C276" modified="2014-01-22" version="1">MTALASRPAKKTSAPAERRAGRGFWHVLGHAAVALLSAAAVVGVYLACVRTPLGQTVDTMMMRGADVNHPRVVEVLDRTLNGTTLASLVLVCVAAAAIGFIRRRVDLALAAGLLVIGANASTRLFKSRLPRPDLDGTGMPNSFPSGHTTAAASVAFALILVLPFAIRGTVALIGAAYVTIIAIATVWAEWHRPSDTVAALFVVLAWGAGASALVRARRARIPGVTARPNRLAMLLFGGVGALSALAALLGIGAVVLSERAVPDLVSGRFAFAAGVAAITAVVAAVFAIWVRLAAGDQPVGDAVTTKAAAHPAPAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3L9</accession>
    <name>U5W3L9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21450"/>
    <dbReference type="EnsemblBacteria" id="AGZ42560">
        <property type="protein sequence ID" value="AGZ42560"/>
        <property type="gene ID" value="AFR_21450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4351"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_38_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="15"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="20680" checksum="79195B7A09351636" modified="2014-01-22" version="1">MDAVAPPRRVRADAERSTARILEAAETVLATEPTASLERIGDEAGLARATVHRRFASRQALIDALISRLNDRYLLAFEQARVRTAPPLVALHRLTEIVFELKLSHRFTVALATGLSPEVETELDQLFTRLHDSGAITAPGTLWRRRIYLSLIHEVAELPADTPELTDGGGTPGARSDLLVRTFLGALNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3T0</accession>
    <name>U5W3T0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_27080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560015.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27080"/>
    <dbReference type="EnsemblBacteria" id="AGZ43677">
        <property type="protein sequence ID" value="AGZ43677"/>
        <property type="gene ID" value="AFR_27080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5488"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_5_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="100"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="18624" checksum="973E0C574CD33F40" modified="2014-01-22" version="1">MRDAHGFDEFYRDTSLRMVRYGLALTGDLAEAQDIVQEAYTRAWRHWRTVAAHPAPEGWMRLAISRLATDRWRRLSGWRAALTRTGPPEPAPPPSEDTVMLTAALRQLPANLRQAVALHYLFDMSVAQIAAETGAATGTVTSWLHRGRTELAAILNAGRPLEVHDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3T9</accession>
    <name>U5W3T9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363016.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21875"/>
    <dbReference type="EnsemblBacteria" id="AGZ42645">
        <property type="protein sequence ID" value="AGZ42645"/>
        <property type="gene ID" value="AFR_21875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4435"/>
    <dbReference type="eggNOG" id="COG3801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138549_1_1_11"/>
    <dbReference type="OrthoDB" id="1817663at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007351">
        <property type="entry name" value="YjbR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04237">
        <property type="entry name" value="YjbR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="136" mass="14953" checksum="B6956F68D27DDF73" modified="2014-01-22" version="1">MATWDDVRRIMAALPATTERAPREWRARDKNLAWERPLRKGDLAALGDDAPSGDILGVRVEDVGVAEALVADDPAVYFTTPHFAGYPAVLVRLEVIEPDELHELLRDAWLARAPKRVVKEFLEGQGLAGGEGTGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3V9</accession>
    <name>U5W3V9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Saccharopine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560156.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27790"/>
    <dbReference type="EnsemblBacteria" id="AGZ43819">
        <property type="protein sequence ID" value="AGZ43819"/>
        <property type="gene ID" value="AFR_27790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5632"/>
    <dbReference type="eggNOG" id="COG1748">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032114_1_0_11"/>
    <dbReference type="OrthoDB" id="1006830at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sacchrp_dh_NADP" evidence="1">
        <location>
            <begin position="3"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Sacchrp_dh_C" evidence="2">
        <location>
            <begin position="144"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="43362" checksum="1325D4F355BD1DBE" modified="2014-01-22" version="1">MRVLLVGAGGVGAAATRIAARRDFYDLFVVADHDFARAQTAVSGLESRGFVAAKVDASSAQAVADLCREHGITHVFNAVDPRFVMPIFDGAYAAGADYLDMAMSLSHPHAEQPFEKTGVKLGDEQFAASGQWLDSGRLALVGMGVEPGLSDVFARYAADHLFSRIDEIGIRDGGNLVVAGYDFAPSFSVWTTIEECLNPPVIWEKDRGWFTTEPLSEPEIFDFPGGIGEVECVHVEHEEVLLIPRWVDARRVTFKYGLGKEFIDVLKTLHKLGLDRTAPVSVGGVSVSPRDVVAACLPDPAGLGDRMTGSTCAGTLVSGTDKDGNPRTVYLYHVVDNEWSMREYGSQAVVWQTAVNPVVALELLATGVWRGAGVLGPEAFDAVPFLEKLVEYGSPWAMREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3X1</accession>
    <name>U5W3X1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cellulose-binding family II protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_28260</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28260"/>
    <dbReference type="EnsemblBacteria" id="AGZ43913">
        <property type="protein sequence ID" value="AGZ43913"/>
        <property type="gene ID" value="AFR_28260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5728"/>
    <dbReference type="eggNOG" id="COG3534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030937_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665667" evidence="3">
        <location>
            <begin position="28"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="482"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="5">
        <location>
            <begin position="566"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="674" mass="69881" checksum="79A5446B1B7ABE50" modified="2014-01-22" version="1">MKILRRALAGTVLLAALTAVAPPGANAAAAPDTTIDVDVRAGLATVPDTALGVNHAIWDSELGSTAVSGLLEDAGVQMVRYPGGSYADIYHWKDHTAPGGYVAPGTDFDTFMAGARRTGAQPMIIANYGTGTAQEAADWVRYANVTKDYGVEYWTIGNENYGNGHYGSAWEADNHADKSPKEYASHVVAYAEAMKAVDPDIKIGAVLTMPGNWPDGIVAAGDQGTWNQEVLSRAGDAIDFVDVHWYPGGGNAAEALTKSEQIVDAVQVLRDQLDKYAGRDVGISLTETNVGVGPNTQPAALFLADVYSALLENGVFTVHWWNVHNGIGTVSTVAGQTDYGDFGLLSSGGCTPDNSVCEPPMNTPFAPYHALSLMGDFARTGDQLVQTGSDNPLVTAHASRRANGDLAVLVINKDPDNAHTVALDYHGFTPAGGAPSVSTFTNGADGVRTGASGTATEQTVPAYSLTLVTVRSGRTGGALPQAPGAPVAGTITDRTATISWPAVAGAGKYEVYRQHNGVSELLGETTGTSFTARNLEPGRRYTVNVLARTNAGALSPSSPPLTFTTGSPADASCTVRLADTSDWGNGFVGSIDVTNNAAGPVSGWTLAFTWPTTWQTVSNGWGATWTQTGTKVRVTSDATLAAGGTTNVGFVGAYSGPNVLPGVFTLNGTVCTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W423</accession>
    <name>U5W423_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phenylhydantoinase</fullName>
            <ecNumber evidence="4">3.5.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_27555</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="PTM">
        <text evidence="2">Carbamylation allows a single lysine to coordinate two divalent metal cations.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.-" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560110.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27555"/>
    <dbReference type="EnsemblBacteria" id="AGZ43772">
        <property type="protein sequence ID" value="AGZ43772"/>
        <property type="gene ID" value="AFR_27555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5585"/>
    <dbReference type="eggNOG" id="COG0044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_2_0_11"/>
    <dbReference type="OrthoDB" id="906155at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="3">
        <location>
            <begin position="50"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="2">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR611778-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="466" mass="50459" checksum="4F82270BE3AB0959" modified="2014-01-22" version="1">MTYLIKGGTVVSPTGASAADVLVDGETIAAVFAPGRGPEDVETIDATGKYVIPGAVDAHTHMQMPFGGTEASDSFDTGTRAAAFGGVTTIIDFAIQRTGEVVQDGLAAWHAKTAGNCHIDYGFHMILGGVTDESLKAMDQLVNDEGISSFKLFMAYPGVFYSDDGQILRAMQKARDNGAMIMMHAENGPAIDVLVKQALERGETDPYYHGVTRPQELEAEATSRAIWLASVAADCPLYIVHLSASKALEQVAAARDQGRNVFAETCPQYLYLTLEDQLGAPGFEGAKWVCSTPLRSKHESHRADLWRGLRTNDLAVVSTDHCPFCMKDQKEMGIGDFSKIPNGIGSVEHRVDLLYQGVVDGKLSLARWVETIATTPARMFGMYPKKGVVAPGSDADIVLYDPNGRTRISASTHHMNMDYSAWEGWEIAGKVDTVISRGTVVVQGDEYKGRKGHGRYVRRGLSDYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4D5</accession>
    <name>U5W4D5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CAAX amino terminal protease family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28935"/>
    <dbReference type="EnsemblBacteria" id="AGZ44048">
        <property type="protein sequence ID" value="AGZ44048"/>
        <property type="gene ID" value="AFR_28935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5863"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052492_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="341" mass="35583" checksum="853F266BF3552BCF" modified="2014-01-22" version="1">MSSTDPTASLDDTRPFGTHIRMAWWKSLIVIAVLPLTLLLLQLVLYLLAGLVEGGDPLESTLTPLKLLAANLSTGLTALLAVVLVSRFAKVPWRRIFSSPGRFALRRLATYFAGSAVLVGVGVAVVAVVVPDGPGWMSFGVSGTTVALLAVVLLSTPIQSAGEEVIFRGAVLPAAGSWVRAVQPAFLTGLVVSSLLFAAVHGAGDPWLISYYVLLSVCTALMGRISRSLEAATGFHVANNLVTTTVNVLLADGGSLVVNRTEGAGGGPSLLVLAAVNLGVLAFVWLRERANRSRSRGLSVVGDYPAAHVDGAVRAEAGQDLRSPALSDVERPAESRGEHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W595</accession>
    <name>U5W595_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560519.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29605"/>
    <dbReference type="REBASE" id="71860">
        <property type="entry name" value="Afr7358ORF29605P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44182">
        <property type="protein sequence ID" value="AGZ44182"/>
        <property type="gene ID" value="AFR_29605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6001"/>
    <dbReference type="eggNOG" id="COG0827">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_269467_0_0_11"/>
    <dbReference type="OrthoDB" id="9701at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044260">
        <property type="term" value="P:cellular macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043412">
        <property type="term" value="P:macromolecule modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1193" mass="133682" checksum="6B47B34AD8C68CE5" modified="2014-01-22" version="1">MIDRKLLLSDLQKQVKTLEKDLREQVESVEEVRLRLGGEYDRAFKVGRTSGTWVVWRDERVTQAAVAWVLGTVFVRFCEDNGLLQEPFLAGPGDRLVLAEEAEAQFFRDQPDKTLRDWLSQGFEAIGGTQAGKSLFDKRHNPLYQIPLSHDAAKGLIGFWRRRGEQGGLVHSFRDELWDTRFLGDLYQDLSEYAKKTYALLQTPEFVEEFILDLTLTPAIDEFGHDQVKMIDPTCGSGHFVLGAFDQLLKQWERHAPNRDAHERVKLALDAVHGVDINPFAVAIARFRLLVAALRASEITTLADAAGYVFPIHLAVGDSLIKHRAGTLFGGDQMTDFAYATEDVAEHPGILTAGSYHVVVGNPPYITVKDKGLNEAYRKMYDACSGKYALSVPFAQRFFQLAKQGDGEGKGAGHVGQITSNSFMKREFGKKMIEKLFALDVDLTHVIDTSGAYIPGHGTPTVILVGRNTNRGRSGVIRTVMGIRGEPSAPPDPSKGQVWHAIRSQVADPGTESEWVSASDTPRVALSRYPWSLSGGGSSEVFKAIGSASPSTLSVTLDGLIGFASFPGADEPYFLPSHWCARAGAGDLAKPLVTGEFIRDWCVVPETMALAPYDGQGSVVAIDMRAVWGQHFWSMRQILRPEARDAFGTSKGDTWWAWYRWIKDRYVADHLLSFASVATHPHVYLDRGGHVFNRHAPVIKLPVGASEDDHLRLLGVLNSSTACFWLKQVSHNKGSTVDSRGARQTTIPWEDFYEFTGTKLQEFPLPTAYPLDVGREMDGLARRLAEVRPTEIAASGVPSRERMFVAEREWHSVRAQMIALQEELDWQVYSLYGLLEDELTAPADSVPELRLGERSFEIVLARKMRDEGLESQWFARHGSTPNTELPGHWTAEYRAAVEQRIAVIESNRNIALIERPECKRRWATEGWDAMQAKALRDWLLDRCEARELWFHHVDGLEQPRPLTTAQLADELRRDEDILSVAGLYAPGQDLGKVIADLVADEHVPHLAALRYKDSGLAKRADWEQVWDLQRQEDTLPDEAAKREFRKKIPVPPKYAPADFLKASYWRHRGKLDVPKERFVSYPGAGRDGDPSLLIGWAGWGHREQAQTLATLIVAREQEDGWAGDRLTPLVAGLREVLPWVRQWHGEFDPTWGESPADTYTGFLSETAGRLHLTDEALTSWRPLKATRGRRARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Q8</accession>
    <name>U5W5Q8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">Crossover junction endodeoxyribonuclease RuvC</fullName>
            <ecNumber evidence="11 14">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">Holliday junction nuclease RuvC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 14">Holliday junction resolvase RuvC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ruvC</name>
        <name evidence="15" type="ORF">AFR_31070</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 14">Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 14">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the RuvC family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="11 14"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560809.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31070"/>
    <dbReference type="EnsemblBacteria" id="AGZ44474">
        <property type="protein sequence ID" value="AGZ44474"/>
        <property type="gene ID" value="AFR_31070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6289"/>
    <dbReference type="eggNOG" id="COG0817">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091257_0_2_11"/>
    <dbReference type="OrthoDB" id="1815080at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16962">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00034">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020563">
        <property type="entry name" value="X-over_junc_endoDNase_Mg_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002176">
        <property type="entry name" value="X-over_junc_endoDNase_RuvC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30194">
        <property type="entry name" value="PTHR30194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02075">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00696">
        <property type="entry name" value="RSOLVASERUVC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00228">
        <property type="entry name" value="ruvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01321">
        <property type="entry name" value="RUVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ44474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="179" mass="18674" checksum="C0E389D1A28D38D9" modified="2014-01-22" version="1">MRVLGIDPGLTRCGVGVVEGVPGRPGKLIGYYVVYSEPDEDIALRLLHLDKSLGELVAEHKPDSVAVERVFSQHNVRSVMGTAQASAIAILAGSRAGLPVQTYTPSEVKAAVTGSGTADKAQVTSMVTRLLGLDAPPRPADAADALALAICHIWRGGTRARMQAAALAAASGRKKGIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Z8</accession>
    <name>U5W5Z8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA polymerase sigma factor rpoD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31690"/>
    <dbReference type="EnsemblBacteria" id="AGZ44598">
        <property type="protein sequence ID" value="AGZ44598"/>
        <property type="gene ID" value="AFR_31690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31690"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860910_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="14434" checksum="597EF35F086B09FF" modified="2014-01-22" version="1">MTLWPTAGLPRSQATQPEAEWGERGVAELANVADDVQRAAALSLQGFTGGSADDMVTADSINPEETRPEAEQGASAGREPSARRSPTGRAAEAGRAWAGRAGILATQCGVGRAKDERVGARPREATRARRPRPTEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W675</accession>
    <name>U5W675_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_32150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AfsR/DnrI/RedD regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32150"/>
    <dbReference type="EnsemblBacteria" id="AGZ44688">
        <property type="protein sequence ID" value="AGZ44688"/>
        <property type="gene ID" value="AFR_32150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6509"/>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3903">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004665_1_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005158">
        <property type="entry name" value="BTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03704">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01043">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="37"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="BTAD" evidence="3">
        <location>
            <begin position="117"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1071" mass="113783" checksum="E28505DC09D56E3E" modified="2014-01-22" version="1">MAVRVAGPGADGLRVRDNQGTRLRVRLLGAVEVRRGDTPVAVPGARLRGLIVRLALAGGRSVEAAVLVGSLWQQELPADPANALQSLVSRLRKLLGDAASVMQSEGGYRLAVTDDDVDALRFERLAASGRERLRAGDARAAAALLGEAVALSSGEPSELAAIAPMVATRLARGAIEASADLAEAELALGRTDAAAARLGALLADHPADERLAALQMDTLAAQGRQADALTLYEQVRETLAERLGADPGAALRERHLRLLQSPASVRPARPAGNLPHAVTSFVGRDDDLARIGTLLARGRLVTVVGPGGAGKTRLAVEAGRRHRHEYRDGTWLVDLAAVTEPDKAGAAVLAAIGVRGAGLFEPSGRMPAGGDELDVLTGRELLLVVDNCEHLIEAVAHLVAALLSRCAGLRVVATSREPLAIDGEALVPLGPLELPDPDGDLEDIRRSASVRLFTERAAAVRPGFDIDERTCADVVRLVCGLDGLPLALELAAARLRTLSLAELAAGLSDRFGLLTTGSRAASPRHRTLRAVIAWSWDLLGDSERTVADRVSVLPGGVTVESAAAVSGVPVAEMPEVLAALVDKSLLQLGGEAGRYRMLETLREYGIERLATTGALDAVRERAARHLAALVAREEPRLRGPDQLAALRVLRAEYDNALAALRRLCDTGDATAAVRLTMDLSWYWQILGRQTDSLYWQAEALGLPAAGHTVDRDTAETMLVLNRINARSPLSVDALRENEAALRVLADRLLAYPSLPGFAGVLTAITLHFLQEHEASIVLVQRLAGGPDVWLSGLAHLFRASFAENEGDLDQVRTDVAAALDAFTRAGDRWGLAGVLPMRAQLRQYDGDLDGALADLREARAYAREFGALSAADEVFLDLRWIDLHLRRGEDTRARALLDGVRERAERSGTPNLSVIVAAQEAGTWLRLGDPDRAEARIEAAEAELSGGGHGQAVVSSVRATLCLHRGDTDGARVALAQAYAAALDSRDRPILALVAVPAAELAGLQGRHRDVARLLGAAARLRGTHDHSDQRIRALTDRGRTALGEAAFAAAYQEGWQLDGKTASRRIDPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6C9</accession>
    <name>U5W6C9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560870.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31385"/>
    <dbReference type="EnsemblBacteria" id="AGZ44537">
        <property type="protein sequence ID" value="AGZ44537"/>
        <property type="gene ID" value="AFR_31385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6352"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="27519" checksum="8DB359E1A8A48575" modified="2014-01-22" version="1">MTNHPRVAIVTGSESGIGRAVAVALAADGYDVGITRYRDAEYAERTADEVRKLGRRAEIRGLDLTRLPQAADVVDELADALGGLGVLVNCAGAGQSSRFLETGWQEWREVLAVDLDGPFLLSQRAAQRLVAAGRGGRIINITSVHETAPRVGAGAYCAAKGGLGMLTRVMAQELGEHGITVNAVAPGEIATAMTGQEDADPTAVSRPGVPLGRPGDAREVAAVVAFLASPAASYVTGASWPVDGGMLLMGPQAGSHLTSDDWRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6D8</accession>
    <name>U5W6D8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560880.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31435"/>
    <dbReference type="EnsemblBacteria" id="AGZ44547">
        <property type="protein sequence ID" value="AGZ44547"/>
        <property type="gene ID" value="AFR_31435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6362"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_4_11"/>
    <dbReference type="OrthoDB" id="1167152at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21204" checksum="44EC366428D13070" modified="2014-01-22" version="1">MDLTIIEAVLFDMDGTLVDSDAAVERAWRTWATEFGADPRAVLAVAHGAPAERTVRLILPGLPEDQVTTAAARQLELQYDDLSDVVPTTGAHELLADLRLPWAVVTSADVRLAKARLGAAGIEAPALVTVDDIRVGKPDPEGYLRGAELLGVSPDRCLVVEDAEVGVTAGRAAGAQVAALKGVDGDLRITDLTELHRLLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6F3</accession>
    <name>U5W6F3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32650"/>
    <dbReference type="EnsemblBacteria" id="AGZ44788">
        <property type="protein sequence ID" value="AGZ44788"/>
        <property type="gene ID" value="AFR_32650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6607"/>
    <dbReference type="eggNOG" id="COG0708">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092367_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="28085" checksum="C56AFEB380C4D0D3" modified="2014-01-22" version="1">MTLRFITWNIKTGGSGGRLDAVVELLRRERPDVLALQELRGFRGQKMREVAAAVGMTPFLAPSFFGQPVAVLVRPPLRIVAWSAVRWRLHHAAAAVVVPTAAGQVTFVSTHLNPFSPGRRLREARWLAARHRPAKGLTLIAGDLNSLDPGTDHTGRLSRLPEPYRRRHLAKDGAADTRAIAAFEAAGFTDLWRTTGEGDGLTVPTTEGGGHEFSEAGMRLDYALATDTVAAAATKARVLHGDEAEYASDHYPLAVDLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6I5</accession>
    <name>U5W6I5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_26590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364420.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26590"/>
    <dbReference type="EnsemblBacteria" id="AGZ43580">
        <property type="protein sequence ID" value="AGZ43580"/>
        <property type="gene ID" value="AFR_26590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5389"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012341_3_0_11"/>
    <dbReference type="OrthoDB" id="856767at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="84"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ43580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="432" mass="44475" checksum="BE6D42DA727BD4F2" modified="2014-01-22" version="1">MIAVALRNLRHRPGGFLATFLSAFLGATLLMTFASLLDTAGGADDASAESLVTTASVAGGWCLIIVVFAVTSTLSLAVRRRADQIALLRRVGATGPQLRRMIVGEAAAVALLAAAVAVPAGLVLGRVLLTLMQDTGQVAAEVDYVYGPIAPALGFGVTLLGAVLAALATSRRTGAGTRRDRPRARRIAGVFFVLVGANCAVLTATLMDGKGIDAMQTAGQAGIWASIGLALWAPELLRLVTALLSVPLRLLGGAPGELAVLRAPASAGAVMPVVLFTGIATGTVYMQAIEDEASDGQVQPDFAQDIQTLNYVVVGMITVFVAIMLVNTLIAATAHRRREFAQQRLAGSTRGQLTAMVAGEALLVTVTGVLTGTAASLFTILPFGSARIGRVWPDTTPTAYAVIVAIAVTLTTLSVLTTTRRTIRGPAVAAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6T6</accession>
    <name>U5W6T6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_33300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561255.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33300"/>
    <dbReference type="EnsemblBacteria" id="AGZ44918">
        <property type="protein sequence ID" value="AGZ44918"/>
        <property type="gene ID" value="AFR_33300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6739"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_11"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005116">
        <property type="entry name" value="Transp-assoc_OB_typ1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03459">
        <property type="entry name" value="TOBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="3"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="350" mass="37145" checksum="F60DDC86927F6554" modified="2014-01-22" version="1">MSLDAHLVVRRPGFTLDAALAAEAGEVVALLGPNGAGKTTALRALAGLIALDDGHIVLDGEQLDDPAGRRFVTPEHRRIGVVFQDYLLFPHLSALENVAFGPRCRGVARADARALADELLDRVGLAEHVRKKPRQLSGGQAQRVALARALATSPQLLLLDEPLAALDARTRLETRAQLRGHLSGHRGPTVLVTHDPLDAMMLADRLVILEDGRVVQTGDAAAITSRPRTDYVARLVGLNLYRGHASGVQVRVGGDFMLTTAAAHEGDVFVAFPPSAVALYGVRPDGSPRNTWQATVLNLSRHGDALRVELTGPILVAADVTPAAAVHLGLELGRTVWASLKATEATSYPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W723</accession>
    <name>U5W723_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Streptogrisin-D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_32720</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561140.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32720"/>
    <dbReference type="EnsemblBacteria" id="AGZ44802">
        <property type="protein sequence ID" value="AGZ44802"/>
        <property type="gene ID" value="AFR_32720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6622"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030648_2_0_11"/>
    <dbReference type="OrthoDB" id="1110723at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004236">
        <property type="entry name" value="Pept_S1_alpha_lytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001316">
        <property type="entry name" value="Pept_S1A_streptogrisin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02983">
        <property type="entry name" value="Pro_Al_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001134">
        <property type="entry name" value="Streptogrisin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00861">
        <property type="entry name" value="ALYTICPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666138" evidence="7">
        <location>
            <begin position="32"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Pro_Al_protease" evidence="8">
        <location>
            <begin position="94"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="347" mass="34266" checksum="438D05A36F9C1592" modified="2014-01-22" version="1">MRNPGSKLGRPIVGLAAVLLVGSAFASPASAAPSVASDPSFAPSALATKLDSQFGTAAAGSYVDSAGKLVVNVTDAATAKSVTASGATPRYVTHSGAALAAADSTLKATLKTPGTSFGLDPVSNKIVVTADETVTGAKLSAVKAIVAKLGDKATFESVSGTLRTTISGGDAIYAGGGRCSLGFNVRNSAGTNYFLTAGHCTNIASTWTNGSTTLGTRSGTSFPGNDYGIVRYTNTTITKSGAVGSQDITSAGTPAVGATVYRRGSTTGLRSGRVTALNSTVNYAEGSVSGLIRTSVCAEPGDSGGSLYAGTTALGLTSGGSGNCSSGGTTYFQPVTEALNVYGVSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7E4</accession>
    <name>U5W7E4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33370"/>
    <dbReference type="EnsemblBacteria" id="AGZ44932">
        <property type="protein sequence ID" value="AGZ44932"/>
        <property type="gene ID" value="AFR_33370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6754"/>
    <dbReference type="eggNOG" id="COG1819">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000537_7_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006326">
        <property type="entry name" value="UDPGT_MGT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01426">
        <property type="entry name" value="MGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UDPGT" evidence="3">
        <location>
            <begin position="271"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="389" mass="41610" checksum="C413F58CFC442F39" modified="2014-01-22" version="1">MVSIPEHGHVNPSLEVIRELVRRGHRVTYANDPSFAAAVRDTGAELRPYASTLPSVTWTDDPIDGLTIFLDDGIAMLPQLRAAYEHDRPDLFLYDIAGAPARLLGEQWGIPAVQLSPAIVAWDGYEQETAPMVEALRSDPRGADYYARFTAWLTENGSSTTDSVAFMGRPARCLALIPRAMQPQADRVDPKVYSFVGPILGDRGTWTRPAGASQVLLVSLGSEFTDHPDFYRRCIEAYGDLPGWHTVLQIGKHVDPAVLGAVPASVEVHPWVPQLAVLEQADAFLTHAGMGGSSEGLFTGTPMLAAPQAADQFDNADRLVELGVARKVDTTTSTAAELRAALLALMADGAVLRRSASLRAELRAQAGAAHAADLIEAELPASIGSSSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7G7</accession>
    <name>U5W7G7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Allophanate hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34350"/>
    <dbReference type="EnsemblBacteria" id="AGZ45128">
        <property type="protein sequence ID" value="AGZ45128"/>
        <property type="gene ID" value="AFR_34350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6950"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014085">
        <property type="entry name" value="Allophanate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02713">
        <property type="entry name" value="allophanate_hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="539" mass="55919" checksum="AA2F1FA2F7F6156B" modified="2014-01-22" version="1">MNDVFQRIRDRGDDGVWISTVGEADLRARAAAVPAGLPLAGLTFAVKDNIDVAGMPTTAACPDFAYVPDRTAPVVQRLLDAGALLVGKTNLDQFATGLTGARSPYGSPESVFGGGLISGGSSSGSAVAVAAGLVDFSLGTDTAGSGRVPAALNGIVGIKPTRGLLSTAGVVPACRSLDCVSIFAPDVARAAAVLRVAKGLAAGDAWGRTGSSPLRSPAELRIGAARSLDFFGDAGQEKAFAAGLDRFGGDVRVVDIEPLLEAGDLLYRGPWVAERLADLGDFLGAHPDSVLPVTRQVLETGLRFTAADAWRGVHRLQELKAWTDRLWESIDVLVLPTIGTTYTHEEIAADPIGRNLNLGRYTQFANLLDLAAVTVPNGFTEDGRPASLTLFGPAFSDDTLALLAGSLTEQDDLMTIAVVGLHLSGEPRNAELTDRRATLIGAARTAPCYRLYSLPSGAPGLVRDTTGASIDIELWQLPAATVGSLLAGIPAPLSLGRIVLADGSEVTGFLCEAYAAAAGQDITASGGWRAHRSSERQDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7M2</accession>
    <name>U5W7M2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34280"/>
    <dbReference type="MEROPS" id="S12.A23"/>
    <dbReference type="EnsemblBacteria" id="AGZ45114">
        <property type="protein sequence ID" value="AGZ45114"/>
        <property type="gene ID" value="AFR_34280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6936"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030169_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="429" mass="46346" checksum="A31FEABA8CB6E57D" modified="2014-01-22" version="1">MDAAGIEAFVAAVAALDDAELHSLMVVRHGHVVAERWWEPYAAQTPHLLYSLSKSFTSTALAFAVAEGLVDLDATVLSYFPEFDARVTDARSRAIRVRHVAAMASGHDDETLDRARVAGDGDLVLGLLLLPPEREPGTFFAYNQPCTYALSAIVQRVSGGSLTDFLRPRLFDPLGISGYGWRKDRLGREIGYAGLYAPTEAIAKLGQLYLRNGEWDGRQLLSPEWVEEATRAHVATVRPEPDWDQGYGFQFWRARHGYRGDGAFGQFMVVLPEADAVVAITSQSPDMQGVLDALWSHLLPALTADGTSALSRPVPGGPVGAIAPALLEPGPGNGVTALLSVEVRPGELVLTDKGPAVTVPLGAPEAWTTTGPVASAYAWADGRLLVDVVFVETPHRLRLSLDLTGERFEAQWQTEPLGGPKLAQLRRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7T6</accession>
    <name>U5W7T6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine kinase, dimerization and phosphoacceptor region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560353.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28775"/>
    <dbReference type="EnsemblBacteria" id="AGZ44016">
        <property type="protein sequence ID" value="AGZ44016"/>
        <property type="gene ID" value="AFR_28775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5833"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="202"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="389" mass="41559" checksum="5A449BF8E7CCD9C4" modified="2014-01-22" version="1">MTLPSRETWWCAVAGLAVLLSGIVEYATQQFLQSFDKGPSITLAVLGTGLAVVLVRRLPWASLALVWIVPIVQTATGTPFVVTQAAFAGVAFGLARWGGTVVVWLSALSIAAGSVIAVVLVAMQAVWVNFSYSSWDLASQAVDQLGVGWRIGLVLVFAAVLGTPWLAGLTLRISDRARIDLGVASRDRVRAEETARLRDAQARLARDVHDVVGHSLAVILAQAESGQYLPDDDPAALKSALATIASSARTSLRDVRGVLTATQDSTDDLDKLIDNLRERGQEVVFRQEGLPRPLSEEQQITGYRVLQEMLTNAIRHGRRGGALIVRRAWLPGAVEVEVRNAVDPGAQDRAESAGSGVEGMRERLTAVGGSLSARADADVYVATARMPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7X7</accession>
    <name>U5W7X7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34245"/>
    <dbReference type="EnsemblBacteria" id="AGZ45107">
        <property type="protein sequence ID" value="AGZ45107"/>
        <property type="gene ID" value="AFR_34245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6929"/>
    <dbReference type="HOGENOM" id="CLU_1286472_0_0_11"/>
    <dbReference type="OrthoDB" id="2044022at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="22847" checksum="EBF6A77A3A076275" modified="2014-01-22" version="1">MITAAGVIKGPWSESAQSARSGQAPAVSTGSILVRVPPGKIVATDKWPHACQLLTDADLAAVLPDATEVESLPGAASTVTIEEYAVDPAWRRDKRTDEGRCAWNMKLPGERVNAYTTVWIRIVAVADPELIRRYHDDQATYRGGGIAPTGLRGGGSCFISSLYAAYLVCAGSALMFEVGGSTTTDFGDYRAYGYWRDTVLPEFAVAVASKIRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8M8</accession>
    <name>U5W8M8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Monovalent cation/proton antiporter subunit MnhG/PhaG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_30155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30155"/>
    <dbReference type="EnsemblBacteria" id="AGZ44291">
        <property type="protein sequence ID" value="AGZ44291"/>
        <property type="gene ID" value="AFR_30155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30155"/>
    <dbReference type="eggNOG" id="COG1320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121334_0_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703">
        <property type="entry name" value="PTHR34703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01300">
        <property type="entry name" value="CPA3_mnhG_phaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="14569" checksum="BC446112C91FCD5D" modified="2014-01-22" version="1">MTAALDVIAAVCLVAGALLSLAAGVALVRFPDLLSRMHAATKPQVLGLLLVLLGCGLRLRDTVDTTTLLLVAVFQLGTAPVAAHMIGRAGYRAGQARADLLLLDELAERADRVNPATSHPRTPAETTAAPPSGRVSDRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9D0</accession>
    <name>U5W9D0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_37820</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562153.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37820"/>
    <dbReference type="EnsemblBacteria" id="AGZ45818">
        <property type="protein sequence ID" value="AGZ45818"/>
        <property type="gene ID" value="AFR_37820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7648"/>
    <dbReference type="eggNOG" id="COG3979">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019399_4_0_11"/>
    <dbReference type="OrthoDB" id="1081028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003610">
        <property type="entry name" value="CBM_fam5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036573">
        <property type="entry name" value="CBM_sf_5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00495">
        <property type="entry name" value="ChtBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51055">
        <property type="entry name" value="SSF51055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666443" evidence="3">
        <location>
            <begin position="33"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="4">
        <location>
            <begin position="116"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="402" mass="41814" checksum="B754A33C99425403" modified="2014-01-22" version="1">MKLSRKYAAALAVVLATAGIGTTVLLAPGASAAAACAPAWSSSAVYVKDNTVSYQAKNYTAKWWTQNEVPTASGEWGVWADKGACGGGTTPPTTPPTTPPTNPPTTQPPATGTKMASAPYIYPGWGNPPAPSTVMNATGIKAFTMAFVLANGCNPVWDGEGGLTGGAHESTINAIKAAGGSVVPSIGGWSGNKLGPNCSTPEALAGAYQKVINAFGLKAIDIDIENTDEFENYAVADRIVGALKIIKQNNPSVKTILTFGTTPTGPNTHGIRLINQSKALGANIDIFTQMPFDFGGGADMYASTVGASEGLKNQLKSTFGWNDAQAYSHMGISGMNGLSDQQEVTSQATWTKIRDYAKNNNFARFTFWAVNRDRGCAGGGVVSDCSGIAQTDWEFTKISAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9F3</accession>
    <name>U5W9F3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562114.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37625"/>
    <dbReference type="EnsemblBacteria" id="AGZ45779">
        <property type="protein sequence ID" value="AGZ45779"/>
        <property type="gene ID" value="AFR_37625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7610"/>
    <dbReference type="eggNOG" id="ENOG50314P5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114045_0_0_11"/>
    <dbReference type="OrthoDB" id="1848269at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="14916" checksum="5B1C5287E33CCF75" modified="2014-01-22" version="1">MTKTLLALHVLAAIVAIGPVTVAASMFPAAARRAASDPGGLPVLRNLHRICRIYAVLGLLVPVLGLATASSLGVLGDAWLITSMVLTVAAAAVLGFLVLPGQSDVLAASPDGALRGAARLGMVTGVFNLLWTVVVVLMVVRPGSTTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9U6</accession>
    <name>U5W9U6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562249.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45914">
        <property type="protein sequence ID" value="AGZ45914"/>
        <property type="gene ID" value="AFR_38300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7748"/>
    <dbReference type="HOGENOM" id="CLU_195021_0_0_11"/>
    <dbReference type="OrthoDB" id="2067425at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="7664" checksum="8CE844A934017792" modified="2014-01-22" version="1">MGAVPAMRKCPRCGHLTRADRMVKGFGRDCATQLGLVGRSVDTGHAGPDLLDLLEQEPEDACDGWDRPNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9V3</accession>
    <name>U5W9V3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HhH-GPD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38645"/>
    <dbReference type="EnsemblBacteria" id="AGZ45983">
        <property type="protein sequence ID" value="AGZ45983"/>
        <property type="gene ID" value="AFR_38645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7819"/>
    <dbReference type="eggNOG" id="COG0122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_72_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="1">
        <location>
            <begin position="47"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="208" mass="22897" checksum="0A5AE1DC9F20382F" modified="2014-01-22" version="1">MDAAERLAAVAELSRREPAFAAVVERFGQPPAWDRVPGFPTLIHIMLEQQVSLVSALAAFNRLRLAVDPLTPEGFLRLDDAQLLAIGFSRQKARYGRALAAALLDGSLDLDALVTRTDDEVDAALFTIPGIGPWTATIYRLMALGRPDAWPVGDIALAQAVAELWNLPARPSQPELLTIAEAWRPHRAMAARLLWHHYLSTPRAPRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9W2</accession>
    <name>U5W9W2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphopantetheinyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_32240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32240"/>
    <dbReference type="EnsemblBacteria" id="AGZ44706">
        <property type="protein sequence ID" value="AGZ44706"/>
        <property type="gene ID" value="AFR_32240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6526"/>
    <dbReference type="eggNOG" id="COG2977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075076_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009366">
        <property type="term" value="C:enterobactin synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009239">
        <property type="term" value="P:enterobactin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041354">
        <property type="entry name" value="4PPT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003542">
        <property type="entry name" value="Enbac_synth_compD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38096">
        <property type="entry name" value="PTHR38096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17837">
        <property type="entry name" value="4PPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01399">
        <property type="entry name" value="ENTSNTHTASED"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4PPT_N" evidence="3">
        <location>
            <begin position="16"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="ACPS" evidence="2">
        <location>
            <begin position="90"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21288" checksum="809C562A7D17938D" modified="2014-01-22" version="1">MVSSRTDPPEAVLFAEEAALVTTAVPKRRQEFTTVRHCARLALASLDVPPAPILPGVRGAPVWPSGIVGSMTHCAGYRAAAVARTTACSGVGIDAEVDGPLPEGVLDLVSSEEERAHLASLAESDPGVQWERLLFSAKESVYKVWSPRTGRWLGFEEAALTFDPAASTFTARILVDGPFETLTGRFLIEDGIVLTAIACAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA23</accession>
    <name>U5WA23_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562207.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38090"/>
    <dbReference type="EnsemblBacteria" id="AGZ45872">
        <property type="protein sequence ID" value="AGZ45872"/>
        <property type="gene ID" value="AFR_38090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7705"/>
    <dbReference type="HOGENOM" id="CLU_1202724_0_0_11"/>
    <dbReference type="OrthoDB" id="2157709at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="25032" checksum="36F68C709DC72432" modified="2014-01-22" version="1">MPYATAEDLRAAAGAFAAAPDRAAARSAAADLAWVAVDDAVAVSPLAAEVVSTARRLVEDGFRWRADAFAVVIHALDHDHVWRDLRAQVRTYRGQYDESIAREQAVAEALQGFDGRVRDLLADPDPETRSLAALLLTRISPEPEADRELLLLLAGADNDDLARGCLGRAILSLGEDGALELLADPSPVTRYRIAHHPWELADSAGRARVLEILGETPLPWESWNWPAEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA44</accession>
    <name>U5WA44_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561144.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32740"/>
    <dbReference type="EnsemblBacteria" id="AGZ44806">
        <property type="protein sequence ID" value="AGZ44806"/>
        <property type="gene ID" value="AFR_32740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6626"/>
    <dbReference type="eggNOG" id="COG4101">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090057_1_1_11"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="54"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="15273" checksum="D96912A3D3C002A0" modified="2014-01-22" version="1">MTDEFHQKLHHIAPLGLSGDTAQTKGMTRIEAISGKTVGSKDLWMGETHVSAATASDNHHHGESETAIYIVRGNPVFVFYDAEKDEETRLETKPGDYIFVPPWVPHREENPDPDNEAVVVIARTTQEAIVVNLPSLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAF2</accession>
    <name>U5WAF2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MDMPI_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33560"/>
    <dbReference type="EnsemblBacteria" id="AGZ44970">
        <property type="protein sequence ID" value="AGZ44970"/>
        <property type="gene ID" value="AFR_33560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6792"/>
    <dbReference type="eggNOG" id="ENOG5032GRJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051661_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017517">
        <property type="entry name" value="CHP03083_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017520">
        <property type="entry name" value="CHP03086"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024344">
        <property type="entry name" value="MDMPI_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11716">
        <property type="entry name" value="MDMPI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03083">
        <property type="entry name" value="TIGR03083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03086">
        <property type="entry name" value="TIGR03086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MDMPI_N" evidence="1">
        <location>
            <begin position="2"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="17349" checksum="960C47DC1C6004E6" modified="2014-01-22" version="1">MVARISPSDLALATPCAEWDLGALLAHMTVQHRGFAAAAAGRGGDPEVWRAGTPSVDAYLRSADEVIAAFAAHDNDFLLPEITARPVPRRMAIGFHLVDYVVHGWDVATALGLGYDLPTEVLAAALPIAEAVPEGSTAFAAPLPDPGGSTFDRILTRLGRSVGPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAL8</accession>
    <name>U5WAL8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">bPH_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_39970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39970"/>
    <dbReference type="EnsemblBacteria" id="AGZ46248">
        <property type="protein sequence ID" value="AGZ46248"/>
        <property type="gene ID" value="AFR_39970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8087"/>
    <dbReference type="eggNOG" id="COG3428">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082134_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="DUF304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_2" evidence="3">
        <location>
            <begin position="154"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="255" mass="28091" checksum="D5C1FA3393E07452" modified="2014-01-22" version="1">MASSGPGYSADAGYSADAGYSPDAGYAGYGTDPRDEDYRDPGEYDDPVFTPEELEGLGGEGAGVAGGPRRVLPLEDEPTTLVSRYLFPTERYRGEWKRHWIHLSTPLLIGIGATLALGYLAGFLTRQNIDGLVTAAVLIWLGVMGWVAWKVGDWYFDRFILTNKRVMVVNGIITRKVAMMPLLRVTDMKYEQSALGRMLSYGTFVLESAGQDQALREVKHLPNPNELYLRVVEEMYEPQAVEARLGKDGDAGDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAM1</accession>
    <name>U5WAM1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_39995</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562586.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39995"/>
    <dbReference type="EnsemblBacteria" id="AGZ46253">
        <property type="protein sequence ID" value="AGZ46253"/>
        <property type="gene ID" value="AFR_39995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8092"/>
    <dbReference type="eggNOG" id="COG1533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015525_3_0_11"/>
    <dbReference type="OrthoDB" id="979208at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040086">
        <property type="entry name" value="MJ0683-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432">
        <property type="entry name" value="PTHR43432"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="8">
        <location>
            <begin position="247"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="INTEIN_C_TER" evidence="7">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="614"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="659" mass="71311" checksum="D46408F267C39B4D" modified="2014-01-22" version="1">MRWSNLSASPDDESLLNAAPAAPPLPLALPGATVRTFDTPGFAGMTFYEVRAKSLINRVPGASRVPFGWTINPYRGCSHACSYCLSGDTPILLADGSTRPLSQLRPGDAVLGTMGAGPHRRYVPTTVLDHWTTSKRAFRVTLADGTRLVSSGDHRFLTDRGWRHVSPAEPGLPALAVGDRMSGVGRFADPPKESPDYRTGYLCGLVRGDAGAPAGSREGDAWVRADTYLEDIPMHVALRWPELPTGSWFRGFLAGAFGATGYADRLAVVFTSRDLGYLDRITDALTHLGLPSRRPPNAQLGGVYSVEIARDLWAALRFRHLTGVTEAPLDASGVGVRSESQLTVADIEDLGLTLPLFDITTGTGDFIADGVVSHNCFARNTHTYLDLDAGHDFDSKVVVKVNAGELIRKELAAPRWQGESIAMGTNVDVYQRAEGRYRLMPQILTALRDFANPFSILTKGTLILRDLPLLRQAATVTDVGLSMSIGFVDEHMWRAVESGTPSPRRRLDAVSKLTDAGFRVSVLMAPILPGLTDTDESIEETVSAIAASGAASVTPLALHLRPGAREWYASWLTREHPELASRYRDLYGNGSYLPKTYQHEITARVRMAARRHGLHRAGAGEARMSGPNAAPATAYADADPTDNAPLAPRHPEPQQLTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAV8</accession>
    <name>U5WAV8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidase_6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561442.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34235"/>
    <dbReference type="EnsemblBacteria" id="AGZ45105">
        <property type="protein sequence ID" value="AGZ45105"/>
        <property type="gene ID" value="AFR_34235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6927"/>
    <dbReference type="eggNOG" id="ENOG5031ZU4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663293_0_0_11"/>
    <dbReference type="OrthoDB" id="983092at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024301">
        <property type="entry name" value="Amidase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12671">
        <property type="entry name" value="Amidase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase_6" evidence="1">
        <location>
            <begin position="240"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="414" mass="44395" checksum="F9D49BE1D9A2B74E" modified="2014-01-22" version="1">MVSFASLRAARPDEWGRAADTWLGLARESEDAANGIFDRGSAKVADNWADQAGILAGEQLGTLADSYLTAAGTMHGVVSVLSGLADSIGVVQRSLEAAVDYALRNGFTIDAEGRVTAPPESASTPEALRDADLLIREALDSATRIDVEARTQLDELAAAVFTTDRDWAVDTVQGNASRSQVELFAEALPIGQDPRTVDAWWDALPPEDRAAFERAVPVQLYDLEGLPADVKARLEGDGGYNRIEAVRWAQQNAFTTDIDIFDNNCANFVSHALANAGLEPKLDDFGGAYSGGWSQGLQTGWDALDAVDYTHTPAWTLSDRQRDFFVENGGAEIPLSGAQPGDVIYWEQAAPGGPIAEGGVHHAAVVTSVTPDGDIHYTQHTASRLDASLDGRLPTNEIVEGDQRPVVVRPRQTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBC6</accession>
    <name>U5WBC6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative secreted chitin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_40975</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562781.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40975"/>
    <dbReference type="EnsemblBacteria" id="AGZ46449">
        <property type="protein sequence ID" value="AGZ46449"/>
        <property type="gene ID" value="AFR_40975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8295"/>
    <dbReference type="eggNOG" id="COG3397">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047929_3_0_11"/>
    <dbReference type="OrthoDB" id="1005693at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003610">
        <property type="entry name" value="CBM_fam5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036573">
        <property type="entry name" value="CBM_sf_5/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03067">
        <property type="entry name" value="LPMO_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00495">
        <property type="entry name" value="ChtBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51055">
        <property type="entry name" value="SSF51055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665887" evidence="3">
        <location>
            <begin position="29"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-3" evidence="4">
        <location>
            <begin position="226"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="174"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="27867" checksum="50B0B736229D2477" modified="2014-01-22" version="1">MRRSIAYPLVALGAVAGSTLVAASPALAHGYVSAPPSRQALCASGAVANCGQIQFEPQSVEAPKGRKSCDGGVAGFSVLSDESRNWPAKAVGKTVTFTWVMTARHRTSNWEYFIGDKKVATVDGGNAQPDAVVTHNIDLSAFPGKQKVLAVWNIGDTVNAFYSCIDLNVGGGGSAPAPAPTTPPAASPTTPPVASPTTAAPTSAVPITEAPPAGGSSGSPAGGSSGSAWAPGVTYKTGDKVTYKGVTYQCRQGHSAIRSWEPSIYTLALWLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBD4</accession>
    <name>U5WBD4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">DNA repair protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">radA</name>
        <name evidence="5" type="ORF">AFR_41320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks.</text>
    </comment>
    <comment type="domain">
        <text evidence="2">The middle region has homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the RecA family. RadA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41320"/>
    <dbReference type="EnsemblBacteria" id="AGZ46518">
        <property type="protein sequence ID" value="AGZ46518"/>
        <property type="gene ID" value="AFR_41320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8362"/>
    <dbReference type="eggNOG" id="COG1066">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018264_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01498">
        <property type="entry name" value="RadA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004504">
        <property type="entry name" value="DNA_repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00416">
        <property type="entry name" value="sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2 3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0346">Stress response</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RECA_2" evidence="4">
        <location>
            <begin position="30"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Lon-protease-like" evidence="2">
        <location>
            <begin position="317"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RadA KNRFG motif" evidence="2">
        <location>
            <begin position="217"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="59"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="426" mass="43914" checksum="D0D5E0189709FBF6" modified="2014-01-22" version="1">MTGPVVSGRVVSSRIPSEPARPIATISAAPAKAVPSGVSELDRVLGGGLVPGAVVLLAGEPGVGKSTLLLDVAQQWAAGTSTPSLVVSGEESVSQVRLRAERLGALHEKLYLASESDLGAVLGHLDAVKPGLLIIDSVQTISAPGTEGVPGGVTQVRAVTAALVSIAKERGVATVLVGHVTKDGSVAGPRVLEHLVDVVLHFEGDKHSSLRLVRGVKNRYGPADEVGCFEMHESGIVSLADPSGLFLTRYNEPVPGTCVTVAMEGRRAMVTEVQALIGAQVQGSPRRTVSGLDSARLAMVLAVLQRRMEKIKLHDREVFAATVGGIRVTEPSADLAMALAVASGALNLAMAPHLVAIGEVGLTGEVRRVGAVGRRLAEAARLGFRFALVPPGCGPGENDSPPKGMRVVEVGDLQSAVQWAARASAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBE2</accession>
    <name>U5WBE2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4032 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41050"/>
    <dbReference type="EnsemblBacteria" id="AGZ46464">
        <property type="protein sequence ID" value="AGZ46464"/>
        <property type="gene ID" value="AFR_41050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8309"/>
    <dbReference type="eggNOG" id="COG3642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035793_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025111">
        <property type="entry name" value="DUF4032"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13224">
        <property type="entry name" value="DUF4032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4032" evidence="1">
        <location>
            <begin position="240"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="415" mass="47133" checksum="6755F2ECADD0B19A" modified="2014-01-22" version="1">MRQRRAVLNVHTGFVRITSALVDPALLDLPWSVPLEEWPASHLVALPQGISRHIVRFVKLNETVYALKETRERIAEREYDLLRALERIDFPAVEAVAVVTDRVDDAGEPLETVLITRHLQFSLPYRALFSHTLRPETMTRLLDALAALIVRMHLTGFSWGDCSLSNTLFRRDAGAFAAYLVDAETGNLYPKLSEGQRSEDIEILRLNIFGECLDLQAAELLHEAIDPETVVDDIVARYERLWHEVTYEQEVARDARHDIEGRIRRLNDMGFDVAEVAMSTVDGGYRVRPKVVDAGYHTRRLLRLTGLDAEENQARQLLNDLDAYRAESDLTDEQQAAHRWLTEVFEPVVRAVPANLRRKLEPQEIFSQIIQHKWLLSERAGRDVGMAPAVQSYLTDVLAHKPDEQAVLGVEVGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBQ9</accession>
    <name>U5WBQ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leucine export protein LeuE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_35700</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561732.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35700"/>
    <dbReference type="EnsemblBacteria" id="AGZ45396">
        <property type="protein sequence ID" value="AGZ45396"/>
        <property type="gene ID" value="AFR_35700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7226"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_3_1_11"/>
    <dbReference type="OrthoDB" id="1927576at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006324">
        <property type="entry name" value="LeuE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="22981" checksum="01CFED448EAC475D" modified="2014-01-22" version="1">MLGITDFWTYVLGTAAIVLLPGPNSIFVLTVGAQRGVRTGYQAAAGVFVGDTVLMVLAAAGVASLLRTYPPVFMVIKYAGAAYLAWLGVGIIRGAWRKWRGRGEPTTVAATTVPAGTQRPFRRALVVSLLNPKAILFVVSFFIQFVQPGYAHPALSFLILGAVLQVFSALYLTALIFGGQFLAGQFRRRRRLSAGVATGVGAVFVGFGIKLATASVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBT8</accession>
    <name>U5WBT8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562984.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41995"/>
    <dbReference type="EnsemblBacteria" id="AGZ46653">
        <property type="protein sequence ID" value="AGZ46653"/>
        <property type="gene ID" value="AFR_41995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8497"/>
    <dbReference type="eggNOG" id="COG5298">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026765_0_0_11"/>
    <dbReference type="OrthoDB" id="470483at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018763">
        <property type="entry name" value="DUF2334"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10096">
        <property type="entry name" value="DUF2334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665905" evidence="1">
        <location>
            <begin position="34"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="545" mass="58388" checksum="5AD25A90E2B569FE" modified="2014-01-22" version="1">MRTSKLTRRALLGAGVAVTATAALGLGVVNADAAVKTTAASTIAAKSGKAAPGGGTAKTLVLYDTTSDYGWMGEVYATQTANLASHFGSWTAAPVANYKANDLAAYTAVVYLGSTYDEPLPAAFLADVTATTKPVTWVFDNIWQLAAADPTFAATHGFTSGAFDFAAVAQVDYKGKKLTRDTTDKSGIMNLSITDPSKVKTLASAVRPDGTTFPWAVQSANLTYVGEMPFAYVTHDDRYLAFADLLFDSLGETGVARERHRALVRLEDVGPDSDPADLRAAADYLYAQKVPFSVAVYPRYRDPKGVQNNGKAQDYTLLQRPKVIAALKYMQQRGGTLLMHGYTHQYGNIANPYDGVSANDFEFYAAHVDANDSVIYDGPVAGDSAAWAASRIAASGLTFALAGLGVPSTFEFPHYAGSAVDYQTVNSVMGKRYDRGLYFPGVLTGAKFDYTRQFGQFFPYAVRDVYGSVVIPENIGNVEPQAFNNHPARLPSDLLASADRNLVVRDGMASFFYHPYLGTDYLKDMVAGVKAQGYTFVSGDSVVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBU4</accession>
    <name>U5WBU4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41650"/>
    <dbReference type="EnsemblBacteria" id="AGZ46584">
        <property type="protein sequence ID" value="AGZ46584"/>
        <property type="gene ID" value="AFR_41650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8429"/>
    <dbReference type="eggNOG" id="COG1716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091241_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31955" checksum="AE77F985665E2666" modified="2014-01-22" version="1">MTQPQGEAPRTSSIAAADGPEPDFFDAPPPAELPEWPPVGAYPGEPGHAPFTPTVTEEQVLEGDLVWHQPPPTEPFHARHEKPGEWRTEPPEHLAWEHSPPAEVEPGPQHDLQTEAIPLGMLPQPWQVPFDPHSTETTVLEAPARQRRTGLWVSLALVLTLLIFGGGTLSAIMLLRDADSGKGAPDPAGAVNRFMTAVYTQQDPASAGDTVCREARDEDKLEDRINQIKGYANEYDAPSFRWNDPAVSAQTEERATVAVQLTMTTDDEKTAQQQLTFTVVHKTGWLVCDVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCJ7</accession>
    <name>U5WCJ7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase subunit b</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP synthase F(0) sector subunit b</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">ATPase subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">F-type ATPase subunit b</fullName>
            <shortName evidence="13">F-ATPase subunit b</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">atpF</name>
        <name evidence="16" type="ORF">AFR_37355</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).</text>
    </comment>
    <comment type="function">
        <text evidence="11 13">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12 13">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562060.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37355"/>
    <dbReference type="EnsemblBacteria" id="AGZ45725">
        <property type="protein sequence ID" value="AGZ45725"/>
        <property type="gene ID" value="AFR_37355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7557"/>
    <dbReference type="eggNOG" id="COG0711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079215_5_1_11"/>
    <dbReference type="OrthoDB" id="1999641at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01398">
        <property type="entry name" value="ATP_synth_b_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005864">
        <property type="entry name" value="ATP_synth_F0_bsu_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="SSF81573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01144">
        <property type="entry name" value="ATP_synt_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 13 14" id="KW-0138">CF(0)</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 13 14" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 13 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="57"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AGZ45725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="19986" checksum="D99C523D22C683E6" modified="2014-01-22" version="1">MLRYLAAEAGESAHNPIIPVWQEIVVGTVAFAVLCFVLMKFVFPMMEKTFAARVDAIEGGIKRAEAAQAEANELLEQYRAQLSEARTEAARIRDEARADAEGIRQDVLAKAREESDRIIAAGRDQLATQRESIVRELRADLGTLAVDLASKIVGESLADEARSRGTVDRFITELGAGTGNGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCX2</accession>
    <name>U5WCX2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563157.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42865"/>
    <dbReference type="EnsemblBacteria" id="AGZ46827">
        <property type="protein sequence ID" value="AGZ46827"/>
        <property type="gene ID" value="AFR_42865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8672"/>
    <dbReference type="eggNOG" id="ENOG5032SMG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124443_1_0_11"/>
    <dbReference type="OrthoDB" id="2016256at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035169">
        <property type="entry name" value="DUF5318"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17249">
        <property type="entry name" value="DUF5318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="17401" checksum="780103EE53AD9C2E" modified="2014-01-22" version="1">MRTQRQVVDYSLQKRAVLRDVHNGKVGTLEVCDASPYLKNAARFHGEPTDDRCPVCRRDNLTLVHYIYGDELKQSAGQARKLAELPVLAMTLREFQVFVVEVCQSCAWNHLIEQYLLGRDALNADELDQGGAVASSASGRHRETGSSSQRREGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDK6</accession>
    <name>U5WDK6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_39105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562409.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39105"/>
    <dbReference type="EnsemblBacteria" id="AGZ46075">
        <property type="protein sequence ID" value="AGZ46075"/>
        <property type="gene ID" value="AFR_39105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7914"/>
    <dbReference type="eggNOG" id="COG1848">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151972_0_0_11"/>
    <dbReference type="OrthoDB" id="1670369at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="14784" checksum="E96D534870B27098" modified="2014-01-22" version="1">MARRKLTHEGTLILDSEGLSKLLADDERVVAMTAEARSRGMEAVISALTIIEAVHARTDKARFNCVLSGLRVVPVGEDEAKAASKLLMNAGLHGHKYAIDAAVAEAALRQHRPVVMLTSDLDDMVKLCGDRVRLVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDP9</accession>
    <name>U5WDP9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46145">
        <property type="protein sequence ID" value="AGZ46145"/>
        <property type="gene ID" value="AFR_39455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7982"/>
    <dbReference type="eggNOG" id="COG1473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PTHR11014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="208"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="415" mass="43448" checksum="BF975929869720EB" modified="2014-01-22" version="1">MTVPDSSHLGPFPGPPPGADPLPGQLDRWLTARGAELVAIRRHIHAHPEPSNAEFETAALVARELAVAGLSPQLLPKGNGLICDIGEGDRVIAFRADLDALPLQDTKDVPYRSTVEKVAHACGHDVHTTVLLGLGLALAQLNEQGELPGRVRLIFQPAEECVPSGAPEVIASGALKDVAAIYALHCYPQLPAGLVGVRSGPFTAAADTIEVKLSGPGGHTARPHLTADLVHALGRVIVDVPALLDRRVDPRAGVSMVWGAVHAGQAFNAIPGEGTVLGTVRILNREAWREAPELITKLIRDVVAATGAEVEVKYNRGVPPVINDRMASAVIAGAAGAALGADRVVEAEISMGGEDFAFYLDNVPGAMIRLGTGTPGSEVKFDLHQSNFDVDEKCIGYGVRVMVHTALAALSTAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE46</accession>
    <name>U5WE46_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1 2">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pth</name>
        <name evidence="4" type="ORF">AFR_39710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PTH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1 2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562529.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39710"/>
    <dbReference type="EnsemblBacteria" id="AGZ46196">
        <property type="protein sequence ID" value="AGZ46196"/>
        <property type="gene ID" value="AFR_39710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8034"/>
    <dbReference type="eggNOG" id="COG0193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062456_2_2_11"/>
    <dbReference type="OrthoDB" id="1676462at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00083">
        <property type="entry name" value="Pept_tRNA_hydro_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PTHR17224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="SSF53178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01195">
        <property type="entry name" value="PEPT_TRNA_HYDROL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="21000" checksum="0351B9A0A7FCA35A" modified="2014-01-22" version="1">MTDEAPFLVVGLGNPGREYAGNRHNVGFMVAELLASRVGGKFGRHKRAVAEVAEGRLGFGGPKLILVKPLTFMNLSGAPVVALSQFFKVPVENVIAVHDELDVPYGQVRVKRGGGEGGHNGLRSMSKSLGSKEYARVRFGIGRPPGRQDPADYVLSDFAGAERKELDFLVDRAADVVEAVVLEGVEWAQNKYHGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFI4</accession>
    <name>U5WFI4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative competence-damage inducible protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563131.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42730"/>
    <dbReference type="EnsemblBacteria" id="AGZ46800">
        <property type="protein sequence ID" value="AGZ46800"/>
        <property type="gene ID" value="AFR_42730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8648"/>
    <dbReference type="eggNOG" id="COG0521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_4_1_11"/>
    <dbReference type="OrthoDB" id="1877633at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="1">
        <location>
            <begin position="5"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="16271" checksum="3C0BDAF596F23CAA" modified="2014-01-22" version="1">MIRARVIVASNRAAAGVYADTSGPLLVAGLREIGCEVDPEPVVVPDGEPVAEALRTALAEGIAVIVTSGGTGVTPTDRTPEATRPLLDFEVPGIAEAIRAYSRDKVPAAALSRGLAGVAGHTLIVNLPGSSGGAKDGLAVLGPILVHTVDQIRGGDHRLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNR8</accession>
    <name>U5VNR8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_00750</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357383.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00750"/>
    <dbReference type="EnsemblBacteria" id="AGZ38439">
        <property type="protein sequence ID" value="AGZ38439"/>
        <property type="gene ID" value="AFR_00750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.151"/>
    <dbReference type="eggNOG" id="COG0641">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_10_0_11"/>
    <dbReference type="OrthoDB" id="1172757at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="PTHR43273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="5"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="367" mass="40888" checksum="3BBBE39085B06C88" modified="2014-01-22" version="1">MRGISTTPTYVVMQPTTLCNLDCSYCYLPFRQVDRKMSVEVARAVAASVNPWSRTGRFSVVWHGGEPLAAGRDHFAALLEPFADDVEHHVQTNATLIDDAWCKFFTERQIRVSVSVDGPEARNGDRVTRGDKPAYARIMRGVEALHRHGIPFSALCVVGNPEPGLATELYAYFLELGCDVLGINVEELEGVNKRDNRHPAAEVTAFWAELVGAWRQDPRIHLREVEWSLRYAAAVLDGTADQVLPRRLDPIPTVAEDGSVVLLSPELAGFHDPRYGDFSSGNVLTTPLGEILERATETPWIREFLDGVEACRARCPYFGFCGGAHAANRYFEQGRFDVTETDHCRNSKIRLLEGVLDHARDHEPTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP99</accession>
    <name>U5VP99_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetracycline repressor protein class E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01625"/>
    <dbReference type="EnsemblBacteria" id="AGZ38614">
        <property type="protein sequence ID" value="AGZ38614"/>
        <property type="gene ID" value="AFR_01625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.327"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="27717" checksum="BC6A78DD703E8340" modified="2014-01-22" version="1">MAEARRTIDLLWFEEPESRRGRKKGLTVAQVIDAAITLADAEGLEAVSMRRVAQELGVVPMTLYTYVPDKAALLDLMLDTIYLRMSREDRPEAPWRERLLAVAEENRLLYASHPWAAGLSRSRPPLGPGLMAKYEHELRAFAGTGLDDVETDSALTYLLGFVQAAARAAEDARLDAERSAQSDQQWWEESGPLLAKVFDTHAYPTAVRIGAAAGAELGGAFNPTHAYVFGLERVLDGLGVLIERRASDPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPE3</accession>
    <name>U5VPE3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01900"/>
    <dbReference type="EnsemblBacteria" id="AGZ38669">
        <property type="protein sequence ID" value="AGZ38669"/>
        <property type="gene ID" value="AFR_01900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.384"/>
    <dbReference type="eggNOG" id="ENOG50337X2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101787_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="262" mass="27668" checksum="6A8971027D3C452F" modified="2014-01-22" version="1">MTDPGWPLIPREVLPAPVQPAQRGRRRTITSVPAGRQLSIFGVEAADPSLADLAGLLAGPGRLGRMGGTARVSVRVDAAWRVHVLVEELVSRGLVVNWAPVAAEPVEEQVAELTAEPFEQTLAPDEDQDQPLETAPVDKEEKAAAATVAFEVRTAYSSRLNGLARAWPPAADKLFLSGPRLRLWVAAAGAPRPQGYALGLDPDGILDKTADAALTRAGLAGTIQEGPAYVIAGRRRLARLAELVGERPSAAPERLWPGSAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPN7</accession>
    <name>U5VPN7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357871.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03195"/>
    <dbReference type="EnsemblBacteria" id="AGZ38928">
        <property type="protein sequence ID" value="AGZ38928"/>
        <property type="gene ID" value="AFR_03195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.643"/>
    <dbReference type="eggNOG" id="COG3386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031076_0_3_11"/>
    <dbReference type="OrthoDB" id="966287at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017996">
        <property type="entry name" value="Royal_jelly/protein_yellow"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009">
        <property type="entry name" value="PTHR10009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03022">
        <property type="entry name" value="MRJP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="364" mass="39540" checksum="E94720496C165793" modified="2014-01-22" version="1">MTEQPSDQPIGDLETVHTFDSGPMPTGVSVSATGRIFVNYPKWGDDVEFTVAELRDGTAVAYPDQAWNSPAGDDDAKAFVSVQSIVVDPADRLWVLDTGSPLFQPTTPGGPKLVCVDLATDTVVQTIVFEPDVALPTTYLNDVRFDLRRNVAFITDSSDQGANGIIVVDLATGTAWRRLHDHPSTKAEEPATFLPVVEGAVLMKRPADGPPEPVRMGSDGIAIAADGSRLYYCPLASRHWYSVSTDALVDRSVGDPEVAATVVDEGNKGGGSDGLETDDQGRFYVTDYEHNAILRRLPDGTFETVAHDPRLLWPDTMSVAADRHLYVTANQLHRQADYQGGTDQRHYPYSLFRVAIDAGPVRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPT1</accession>
    <name>U5VPT1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02640"/>
    <dbReference type="EnsemblBacteria" id="AGZ38817">
        <property type="protein sequence ID" value="AGZ38817"/>
        <property type="gene ID" value="AFR_02640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.531"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_47_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="89" mass="9672" checksum="48C058413D7FC678" modified="2014-01-22" version="1">MRIDSIGDLGLFVRDERRRQGLSQEELAIRSGTSRRWLSDLEGGKVTVEAGLTLKVIAALDLILEVGPAPTPEIDLDAFLDNLGGSDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR47</accession>
    <name>U5VR47_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_05175</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358851.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05175"/>
    <dbReference type="EnsemblBacteria" id="AGZ39324">
        <property type="protein sequence ID" value="AGZ39324"/>
        <property type="gene ID" value="AFR_05175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1047"/>
    <dbReference type="eggNOG" id="COG4124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038234_1_0_11"/>
    <dbReference type="OrthoDB" id="669354at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665655" description="GH26 domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="5">
        <location>
            <begin position="29"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="332" mass="36911" checksum="1BC3913C5F9E1679" modified="2014-01-22" version="1">MRRVLALLGLLVLVLAGCTSNRRAEPEPTPSPDLTSAPIAVAVNPGPFESGPFEAPSTGAYLGAWIKPDALTHVGRVEAVAGLEEDLGRPLDIINTYRRFEQMVGTESDAEFLDQGATLMVSWATGDNRSILAGEHDRLIKQQAKAIRKVKKPVLLRIRWEMDRPNLRATMWSGEDYIAAYKHIRAIFAEQRVKNVSWVWCPTAEGFIRGDAPAFYPGDDQVDWTCVDVYAGNVFQPIGQLMGPFLQWAAQRPKPIIIGEFGVAKAWGSQGRADWLKNAARTFKANPQIKGVVYFESNPEGNGPKQQFQLAGDRPAFREFTKLTKDPYFNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR53</accession>
    <name>U5VR53_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05820"/>
    <dbReference type="EnsemblBacteria" id="AGZ39453">
        <property type="protein sequence ID" value="AGZ39453"/>
        <property type="gene ID" value="AFR_05820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1182"/>
    <dbReference type="eggNOG" id="ENOG5032URV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108513_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="18124" checksum="104DEB4FC9C0A3B8" modified="2014-01-22" version="1">MPITDLVRVYVPATVPMLAEMRTTGQLGAGPVVAHAVTPALREWYAEGDEEELEYVAFTRAAQAALQLLRHDPKAPRRRVVVSADVASTALVREDVELGSSTVRLPQPISLKEVASIHLDGLSAAAEIGAAADVVEEALAGDPDAQFTVDGAEDHELEWYAVTELDELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRI2</accession>
    <name>U5VRI2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357284.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00255"/>
    <dbReference type="EnsemblBacteria" id="AGZ38340">
        <property type="protein sequence ID" value="AGZ38340"/>
        <property type="gene ID" value="AFR_00255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.52"/>
    <dbReference type="eggNOG" id="COG1922">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063203_2_0_11"/>
    <dbReference type="OrthoDB" id="1759731at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06533">
        <property type="entry name" value="Glyco_transf_WecG_TagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004629">
        <property type="entry name" value="WecG_TagA_CpsF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34136">
        <property type="entry name" value="PTHR34136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03808">
        <property type="entry name" value="Glyco_tran_WecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00696">
        <property type="entry name" value="wecG_tagA_cpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="273" mass="29827" checksum="84E42786A1183D58" modified="2014-01-22" version="1">MGSAVVTAGGKKNVLGVLVDATDYDTATAQIIEAARERRHYAVTALAVHGVMTGVQDKAHNARLNSFDLVTPDGQPVRGALNLLHHAGLTDRVYGPELTLRVLRQAAAEGLPVYLYGSTEPTLEKLVPALERMFPALKLAGVEASKFRKSHPGEPAEIAERIKASGARIVLVGLGCPRQEIWAYAMRPLLDMPLLAVGAAFDYHAGLLKNPPAWMQKYALEWLWRLGLEPKRLWKRYILLNPAYLSRLALQKAGIWKATPPAPTSEPVTDFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRU6</accession>
    <name>U5VRU6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent glycerol-3-phosphate dehydrogenase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359080.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06300"/>
    <dbReference type="EnsemblBacteria" id="AGZ39549">
        <property type="protein sequence ID" value="AGZ39549"/>
        <property type="gene ID" value="AFR_06300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1278"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025711_4_5_11"/>
    <dbReference type="OrthoDB" id="948454at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="9"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="208" mass="22690" checksum="6A2B14B8659EC2EF" modified="2014-01-22" version="1">MSLNIVVLGAAGRTGRLIVAEALHSGHQVTAAVRSPASVPAAPGLRVERADVRDADNLRAVIQGHDAVVSAIGASGRKADNLYSDGARATVSAMQATGVKRLLAITSVGVRRDDPHHAWWYRGLIRPIGADLYADMTRMEQIVRDSDLDWTFVRPTYLQDREPTGGYRATDNSTPQGGWRITRTDVARFIVEELDAHRWSRQAPSLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS20</accession>
    <name>U5VS20_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_07445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359309.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07445"/>
    <dbReference type="EnsemblBacteria" id="AGZ39778">
        <property type="protein sequence ID" value="AGZ39778"/>
        <property type="gene ID" value="AFR_07445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1520"/>
    <dbReference type="eggNOG" id="ENOG50301FA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136600_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666012" evidence="2">
        <location>
            <begin position="29"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="162" mass="16068" checksum="835C1C9D18A0244D" modified="2014-01-22" version="1">MRTSVPAALAAIALAMPVVLLESSPALAADVFVETNPSTVRAGDDIGIRASCDDNLKPATVTAGPIGTVTVSPSYGFLTTTTRVPSATDPGDYPVRLKCPDGKTAENTLHVVVKVEPARGPATGGGGTAEGRSAPLLIGGGLAALGAAAALGVAAARRRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS50</accession>
    <name>U5VS50_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alkaline phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_07620</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359344.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07620"/>
    <dbReference type="EnsemblBacteria" id="AGZ39813">
        <property type="protein sequence ID" value="AGZ39813"/>
        <property type="gene ID" value="AFR_07620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1553"/>
    <dbReference type="eggNOG" id="COG3540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525464_0_0_11"/>
    <dbReference type="OrthoDB" id="165142at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032093">
        <property type="entry name" value="PhoD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16655">
        <property type="entry name" value="PhoD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665163" evidence="1">
        <location>
            <begin position="30"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD_N" evidence="3">
        <location>
            <begin position="50"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD" evidence="2">
        <location>
            <begin position="155"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="519" mass="56532" checksum="70DEE5A7E73C16A3" modified="2014-01-22" version="1">MTFIDRRSLLRAGLAGGVLGALPAGSALAGPAPVAPALGGRARHRPVLTHGVQSGDATAHSAVVWARADRPGRMWVEASRRPDLRGARRVRGPILTPGTDFTGKVRLRGLPAGEKIYYRVHVEGLDHHAGEPLTGSLSTAPDRRQDISFVWTGDVVGQGWGIDPAYGGLKLFESMRARRPDFLLHSGDTVYADGPLVPSVTLPDGRIYRNLVTEEKSKVAETLTEYRGQYAYNLLDDAYRAFAAEVPQINQWDDHEVTNNWYPGEILDDARYTEKRVDVLAARAHRAYHEWVPIPSQSGPVHRKISYGPLLDVFVLDMRTFKDVNDGNTYADPNRGLLGTEQREWLIRGLKNSKATWKVIANDLPLGIVVPDGPTAQEGVAQGDPGAPKGRELEFAQVLQSAHRAGVTGIVFLTADVHYTAAHHYDPARAAVQDFTPFWEFVSGPAHAGAFGPNALDATFGPNTAFVHAPPVANTSPADGYQHFGEVRIDGRTGAFHVDLRDREGKSLWSTTLPAPHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSA0</accession>
    <name>U5VSA0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_07225</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359265.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07225"/>
    <dbReference type="EnsemblBacteria" id="AGZ39734">
        <property type="protein sequence ID" value="AGZ39734"/>
        <property type="gene ID" value="AFR_07225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1475"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="22914" checksum="A2C0E98AE0F659E0" modified="2014-01-22" version="1">MSDVIELSGATKSYPGGVTALDHVDLAVGYGELAAVVGPSGSGKSTMLHLIGTLDRPSAGTVRIDGHDVARLSDRQLSALRARRIGFVFQQFHLAPGRTAVANVADGLLYAGVPGKERERRAEAALHRVGLGGRLGHRPHQLSGGERQRVAIARAVAGDPALLLADEPTGNLDSVAGAGVVDLLRELHAAGTTVLVITHDRDLAADLPRQITMRDGRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSL8</accession>
    <name>U5VSL8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase E</fullName>
            <ecNumber evidence="6">2.1.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_07850</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) + N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42920"/>
            <dbReference type="Rhea" id="RHEA-COMP:10283"/>
            <dbReference type="Rhea" id="RHEA-COMP:10284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74502"/>
            <dbReference type="EC" id="2.1.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the RNA methyltransferase RsmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.193" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359390.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07850"/>
    <dbReference type="EnsemblBacteria" id="AGZ39859">
        <property type="protein sequence ID" value="AGZ39859"/>
        <property type="gene ID" value="AFR_07850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1603"/>
    <dbReference type="eggNOG" id="COG1385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067442_2_0_11"/>
    <dbReference type="OrthoDB" id="1490892at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006700">
        <property type="entry name" value="rRNA_ssu_MeTrfase-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30027">
        <property type="entry name" value="PTHR30027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04452">
        <property type="entry name" value="Methyltrans_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015601">
        <property type="entry name" value="MTase_slr0722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00046">
        <property type="entry name" value="TIGR00046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25523" checksum="8E42A6D9AC2C2A8E" modified="2014-01-22" version="1">MSAPLFLVDDLPGGKRFTLAGPEGHHAATVQRLKAGEVLVLGDGRGGTVTAEVTGVGRGSVDVEVRDRAFQAAPDPRLVVVQGIAKGDRGELAVQAMTEIGVDEIVPWAASRSVAQWRGDRGERAREKWVATAREAAKQARRSWLPVVSGSPDVSTKKVAARLSEVAGAFVLHEEATDRLTGAKLPATGEIMLVVGPEGGISDQELGAFVAAGAHPVRLGDSVLRTSTAGMAALAVLSARLERW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSS1</accession>
    <name>U5VSS1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357624.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01960"/>
    <dbReference type="EnsemblBacteria" id="AGZ38681">
        <property type="protein sequence ID" value="AGZ38681"/>
        <property type="gene ID" value="AFR_01960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.397"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_18_18_11"/>
    <dbReference type="OrthoDB" id="1791095at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="12"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="2">
        <location>
            <begin position="168"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="488" mass="53560" checksum="FF1D8739AF5849FD" modified="2014-01-22" version="1">MQSAALTRTTLRAVLYLRMSVDRTGNRAALKSQELACRALALARGWEVVDVVDDTVSATTVRLADRPGWQRVVRKIEAGEADLIVAWHLDRVTRSLKDLEFLIDLALERDIGLATATGDIDLTTDVGRMVARILAAVATAETERKAERQILANDLRVSTGRPQWIRRPFGYELDGTLRRAEAAAVLRAYQDVLAGKALTTVAREWNEAGFRTSAPDPATARARAPRRNDYDPSGRWSNVAVRSLLRAPRNMAKSTLYGEIMGDGNWTPIVDETTWRAMDLFLAGRERPGSTVGKLATLLSRIASCTVCGGPMGASKRGSAPIYVCQGPVDETLSRGHCQLPLDFADRVVVSRLLQQMEATGRTMSGQQPAPMDVAPLKARENEIELLMAELVEDRAAGLLERAAVLTGTARLREELAGIRKSVLEAGRRETADEEFGKLALVEQRAVLRDAFTFIRVIPRGKGRPKAGQPTWRAALIETEFTPAWSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSV1</accession>
    <name>U5VSV1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02160"/>
    <dbReference type="EnsemblBacteria" id="AGZ38721">
        <property type="protein sequence ID" value="AGZ38721"/>
        <property type="gene ID" value="AFR_02160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.437"/>
    <dbReference type="eggNOG" id="ENOG5033HR0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135691_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665649" evidence="1">
        <location>
            <begin position="23"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="16202" checksum="132A5C0B72334D71" modified="2014-01-22" version="1">MLATALLALLCLPCAVAVVVCADELVARRTWTRHGRREIRALRCLERDLTSADLESRPPAPEPDMAELVADLRRLNRQRRSGPTRHSATWLAAVLEAYDERLELASRHLGLDEHLAQLEGMDREIERVRVEGLLHAAGLRLRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT68</accession>
    <name>U5VT68_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, DeoR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_03180</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SorC transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03180"/>
    <dbReference type="EnsemblBacteria" id="AGZ38925">
        <property type="protein sequence ID" value="AGZ38925"/>
        <property type="gene ID" value="AFR_03180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.640"/>
    <dbReference type="eggNOG" id="COG2390">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054506_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007324">
        <property type="entry name" value="Sugar-bd_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04198">
        <property type="entry name" value="Sugar-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r4" evidence="3">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar-bind" evidence="2">
        <location>
            <begin position="61"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="31831" checksum="0158D1A856061E32" modified="2014-01-22" version="1">MQDLIAALVARRFYLEQRTKKQIGDELGISRFRVARILDRALRDGVVRIEIAVPPAVDLELSERLTAYTGIRQALVLREPDQLGPACAALLAERLTEEDVLGVSWGRTLHELTAVLPPLPRAEVVQIVGSVPSADLNVNSLELLRRLSERTGGRVHALHVPMILDSPEVAASLRATASVAETLATFPRLTCALVGIGRWQPGGSSLRSALPEPLIRDLDAAGAVADICSTVLDKNGSVVGGDTVPARCIAITTEQLRAVPDVIAVAGGETKAPALAAAVRAGLVHRLITDTAAATALMSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTC9</accession>
    <name>U5VTC9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09770"/>
    <dbReference type="EnsemblBacteria" id="AGZ40243">
        <property type="protein sequence ID" value="AGZ40243"/>
        <property type="gene ID" value="AFR_09770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1992"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_6_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="22207" checksum="6E3684FEE797673F" modified="2014-01-22" version="1">MVVGAGVRRQQAAQTEAELKAAAVRVFARLGYLNTKITDITAEAGRAAGSFYTHFANKEALLEALLTDLLARGDVSAVDEQHDDDFSRRDAVRYHVALYWTFSRENRTLLSALQQAATVDAGFAERSRALVEPDIHHIADHLTRAAEAGFRLPGDPLVVATIFTTLVPAFSAMWQSGEGPALGRELSDDEAIETLTSFIYSGLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTJ5</accession>
    <name>U5VTJ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative LysR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360078.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10145"/>
    <dbReference type="EnsemblBacteria" id="AGZ40318">
        <property type="protein sequence ID" value="AGZ40318"/>
        <property type="gene ID" value="AFR_10145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2066"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_11"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="297" mass="32301" checksum="57B125DA6261F348" modified="2014-01-22" version="1">MIDLGRLRALHAVASYGTVLAAGEALHCTPSAVSQQLAKLERETGATLVEKDGRRLRLTEAGRVLADHAERVLATVDEAEAALAAHRDTVVGRLTVAAFATACRALLPYALHRLATDHPQLTTGLVEVNPHEGLEVLRRGHVDLAVLDDWPEVALRYPPGVSHVEIGRDVADLIVPAGHRFATAREVRLSQARDERWITAKAGDVCHEWLVRMLPGVRPDFNVGEFETQLTLIAAGLGIAIIPRLARPPLPQGVCVLPVRPVPTRRVVVAWREASAARPAITAAITALREAWRRHET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTL1</accession>
    <name>U5VTL1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Triple helix repeat-containing collagen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09550"/>
    <dbReference type="EnsemblBacteria" id="AGZ40199">
        <property type="protein sequence ID" value="AGZ40199"/>
        <property type="gene ID" value="AFR_09550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1947"/>
    <dbReference type="eggNOG" id="COG5373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_637175_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="430" mass="41134" checksum="368C2619D4922905" modified="2014-01-22" version="1">MSPADLDEVFDDEPEATTDARVSGDPWAAATRAPRRPTRKPTGAATPPLTNATPAAPSTASTPAPAGEPRTATNPGLATEPVPATEPGPGTEPGPATEPGPGTEPGPATEPGPATEPGPGTEPGPATEPGPGTEPGPATEPGPATEPGPATEPGPGSEPGPATEPGPGTEPGPGGEPGPGNEPGAATEPGPRNEPGAATEPGLRSEAGLATEAGSGSESDAKVASGRAGQVGSAFERSQSRGAGGDPWAEATRNPAPSGRAAGSVPAAEPATGKAGRHAADRGEDADPVEHQNPGVVVEGAAVRTGKEAGQSDAGQGYPGVVVEGAAVRIGKEAGPSDAEQGYPGGGVAGAAGRVGGVEGVGERSGFSGGIPNTELESSENQVNHDTEGYGAVADGLGVDHDVAGTAPAASAAEDRDGGAGDDARSGGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTN4</accession>
    <name>U5VTN4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40224">
        <property type="protein sequence ID" value="AGZ40224"/>
        <property type="gene ID" value="AFR_09675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1972"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="30"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="33090" checksum="530F52DCE86D2E96" modified="2014-01-22" version="1">MSGNELGLFLRTRREAIAPAEVGLPVGPRRRLAGLRRSEVAALAGVSVEYVTRLEQGRDRRPSPPVLAALGDALRLTPPERAHLYRLSKGADTGFTCRGQDSRAREVRPTIQALLQTLEPSLAVLVDHVGEVLACTPQYRTVAGATGLLDGSPPNLVRFVFTDSRARTAYPDWDQVADDQVATLKQGPFRADPDIAALVDELTITAGDIFTRRLATVPGLAPATGVLRLWHPEARELRLAYETLELPADDGQRLLVHLPADLATSAALYRLTTPPPGAPHPYAVFGPVRTDPPDPDQRRPGLRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU19</accession>
    <name>U5VU19_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_04135</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39116">
        <property type="protein sequence ID" value="AGZ39116"/>
        <property type="gene ID" value="AFR_04135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.835"/>
    <dbReference type="HOGENOM" id="CLU_417780_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666089" evidence="3">
        <location>
            <begin position="25"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="656" mass="62585" checksum="2F3CE32D42755572" modified="2014-01-22" version="1">MVFAGAAFAGAAFVAVVFADAAFAAAAFVGAALVTAVLVPAVLVPAVPVVAVPVVAVPVVAVPVVAVPVVAVPVVAVFVAAVFAGAAFAVAALTVVPFAVAVFVVAVLAVAVLAGVALVAGVLADTAFAAAALPGAAVVPATFVVAAFAAVGFAPVAEAAADFAGARVPAAVFTGAAFAAADDAAEPAVVLPAVAPAVADLLDPVLLVAAALVAARPAEAADLAGPAPARADFAGGVVRSVTADLFGVDLAATARAGVPAPVPVDSAGRLDRVTRAALTEPIDVIPEAAADFATSDTAAEPVGPADFVAADLAEPADFAVADLAGPADFATADLAGSDDFAVASTTGSADFAAAGLAWRAGFAAGRAGPETSATPDVSGLDARPADFAPGRAALIGFGDSGSFGERGRPATFVAALAASVTRASGLGASAAGAAAALTAAGFRRRMGVGSAPAAAAVRRVRAAGLASAAPDASKAVDAGEAADVGAAVSGDSGMAGVPVGLSGDAFWARPAAVALGRRALRPAATALRAEPAGPSAEAAGSRTDAAGPSTGAAGSRTDAAWPKAEAAGLSAVTAGSGAEADLRRAETGGLPAEGGGLRRTVAVVWSRVGRSSAAARGAAEPFVAFAITYARVPEAGGRHRRFARPPQNHGGGRPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUB6</accession>
    <name>U5VUB6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-binding region ATPase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_11270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11270"/>
    <dbReference type="EnsemblBacteria" id="AGZ40543">
        <property type="protein sequence ID" value="AGZ40543"/>
        <property type="gene ID" value="AFR_11270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2296"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017312_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="4">
        <location>
            <begin position="267"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="495" mass="52599" checksum="412A74EF105D1A6D" modified="2014-01-22" version="1">MPHIPPSAVPATDDVALRGRLTWLIAAPAGAAALLFAVTAGYFWAGGADTSDLVLVVAFLLGVLALVVVAAKAAGTVRQLDDRVAPLLSEAATARAEAATARAEADAARTGLADAQTRAASAKDEATGARTALDDARAELTRLAEQLQVAEQRPVPVRTSAGRQVEVFVNLSRRLQSLVHREIKLLDALENEVEDPDLLKGLFQVDHLATRIRRHAENLAVLGGAVSRRQWSRPVALTEVLRSAIAEVEHYSRVKLVPPIEGTVPGHAVASIIHLVAELIENATMFAPPHTQVLLRAQHVAAGVAVEVEDRGLGMQRDDQERVNRLLLNPDDINLDELLADGRIGLFVVSMIARQHGVTVQLQGNIYGGTQAVVILPQHLLGSAQADPEPTGTRLALSQNTMELAQRIPGPSQIAAAPPVWPAFAPPAEVPEPVAVTTPPPLPRRHSQTHLAPELQAGPPAAAPDDDTEHNTGLMASFMQGVSRSEAADQPDENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUG6</accession>
    <name>U5VUG6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminopeptidase N</fullName>
            <ecNumber evidence="4">3.4.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_04960</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.</text>
            <dbReference type="EC" id="3.4.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.2" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04960"/>
    <dbReference type="MEROPS" id="M01.032"/>
    <dbReference type="EnsemblBacteria" id="AGZ39281">
        <property type="protein sequence ID" value="AGZ39281"/>
        <property type="gene ID" value="AFR_04960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1002"/>
    <dbReference type="eggNOG" id="COG0308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014298_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="SSF63737"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M1_N" evidence="12">
        <location>
            <begin position="10"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M1" evidence="11">
        <location>
            <begin position="259"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ39281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="405" mass="43757" checksum="AE68D7E8BAA4381E" modified="2014-01-22" version="1">MRYDPASGELGGTATISATATQDLARFNFDLARLKASKVTVDGQAATSAADGDELVVTPAAGIRTGTAFTVVVDYSGKPEALNNEALGVGGWLRTKDGAFALGQPESASTWFPVNDHPSDKATLALSMTVPDGTEAIGNGVPGPRETAGGWTTWTWKEQKPLASYLAFVAIGQYRVQTSTHKGKPMVIAIPESLPPTSNAARAMARTGEVADYLETQFGPYPFDAYGGVVLDDDRVGYALETQSRPVYGNTFFKGGADVSVVAHELAHQWYGDSVALDRWQHIWLNEGFATYAEWLWSEHEGGRTVQQSFDRVYAAFDWSTPPGDPGAKRLFSDAVYIRGAMTVHALRKAMGDKAFFALLKSWPADNRDGTVTTEDFIAAAEKASGKDLGDLFEQWLFGKTRPTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUQ9</accession>
    <name>U5VUQ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain dehydrogenase/reductase family oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360782.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12185"/>
    <dbReference type="EnsemblBacteria" id="AGZ40723">
        <property type="protein sequence ID" value="AGZ40723"/>
        <property type="gene ID" value="AFR_12185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2477"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_0_11"/>
    <dbReference type="OrthoDB" id="968232at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05324">
        <property type="entry name" value="carb_red_PTCR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045313">
        <property type="entry name" value="CBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25663" checksum="9374E8FB47F125BF" modified="2014-01-22" version="1">MTTTQQTALVTGANKGIGFETARQLAQLGFTVWLGCRDQGRGEAAAKELAADGDVRFVRLDLTDTVSIQAAQEHIAEHSGTLDVLINNAAIALGSQEGPPSTMRPETIEKTLEVNFYGTLHVTQAFLPLVRKAEAGRIVNLSSSMGSLTMLTAPEQPLAPFGVSYAYSSSKTLVSTLTGWLAVELKDTPVKVNSVCPGFNATDLNGNMGTQHPSEGAKVVVRAATLPADGPSGSFFDDAGPVSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUS3</accession>
    <name>U5VUS3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative type IV pilin PilA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359016.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05980"/>
    <dbReference type="EnsemblBacteria" id="AGZ39485">
        <property type="protein sequence ID" value="AGZ39485"/>
        <property type="gene ID" value="AFR_05980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1215"/>
    <dbReference type="eggNOG" id="COG2165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091705_7_1_11"/>
    <dbReference type="OrthoDB" id="1962095at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000983">
        <property type="entry name" value="Bac_GSPG_pilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00813">
        <property type="entry name" value="BCTERIALGSPG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="159" mass="16736" checksum="619284FF57F46D10" modified="2014-01-22" version="1">MQEIIARMRAKKQDEGFTLIELLVVVVIIGVLVAIAVPVYLNYRQGAADKSAQSDVRGAVSAVEQFYTDNGNAYPAADVTVDNSAGATSSKELATGSAQKFTVSDKTTLQYHLSTDKLSYKLCAVNAGGSKKYYLYDSSKGGSVAASTAITTANFATCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUT8</accession>
    <name>U5VUT8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase pa-phosphatase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360624.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11600"/>
    <dbReference type="EnsemblBacteria" id="AGZ40609">
        <property type="protein sequence ID" value="AGZ40609"/>
        <property type="gene ID" value="AFR_11600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2364"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072573_3_2_11"/>
    <dbReference type="OrthoDB" id="1928533at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="97"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="24939" checksum="1F9EE5DB5D8B5B99" modified="2014-01-22" version="1">MADFAVQVLKRVLAPIAVLLAVMIGLGLLVTKVLMHVWPLTVEDSVNREFERDRTHEISLFSLIFSTLASTQAVIAVTVLAAIVLRLTLHRWRESIFLCAAVCAQALIFFLTTLVIDRPRPSVDHMDVSPPTSSFPSGHTSASVALYLGLAVILYRLTDKIWLKRACWLLLLIPIGVALGRMYRGMHHPSDVTASFLNGGICVWVMARAILDRGAIQARRLTSARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUZ1</accession>
    <name>U5VUZ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360733.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40674">
        <property type="protein sequence ID" value="AGZ40674"/>
        <property type="gene ID" value="AFR_11925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2428"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="149" mass="15598" checksum="DAA9C160FDC2A0D9" modified="2014-01-22" version="1">MSTNDDVDLNLGRDLSSAVVLFHQAVAARVGLSAGDLKTLELIATEGPFSASDLARRTGLTGAGITSVIARLTAGGHVVRETDPGDRRRAVIRAAETGHPQLAGAFADLGRALGGVITEYDPAEQAAIVSYLRRMVDILREQTIHLGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVB8</accession>
    <name>U5VVB8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lactamase_B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359092.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06360"/>
    <dbReference type="EnsemblBacteria" id="AGZ39561">
        <property type="protein sequence ID" value="AGZ39561"/>
        <property type="gene ID" value="AFR_06360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1290"/>
    <dbReference type="eggNOG" id="COG1234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031317_3_0_11"/>
    <dbReference type="OrthoDB" id="1712770at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="18"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="248" mass="26150" checksum="3C961A098057828D" modified="2014-01-22" version="1">MRLTVLGCAGSFPGPESACSAYLVEAEGFRLLIDFGSGSLSALQRYAGIGAVDAIMLTHLHCDHMLDACTYVVVRRYAPGGPLPPLPVYAPMGAAERISAAYSSENEPVDDVYTFYGLQPGTFPIGPFTVTADRVNHPIETYGVRVEHNGKVLAYSSDTAPCDSLLRLAQGADLFLCEASYLDGVENPPDLHLTGGEAGEAATKADVGKLLLTHLVPAWVSEASTVEAASAAYAGPVEVVRPGSRYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVD2</accession>
    <name>U5VVD2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_06880</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359196.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06880"/>
    <dbReference type="EnsemblBacteria" id="AGZ39665">
        <property type="protein sequence ID" value="AGZ39665"/>
        <property type="gene ID" value="AFR_06880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1405"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DUF4162"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DUF4162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="35116" checksum="44F59839221DF6B1" modified="2014-01-22" version="1">MIETHELTRHFKDVHAVQGINLTVAPGELVALLGPNGAGKSTTLRMLTTLIPPTSGTAEVAGFDVVRQQREVRQRIGYIGQGNGAAHSQRGRDELISQGRAYGLKVAAARERAGELIDSLGLGEVADRTVSTLSGGQRRRLDIAMGLIHAPELLFLDEPSTGLDPQNRANLQEHIMQLRKQHETTIVLTTHYLDEADSMAERIIVIDHGRIIADDTPARLKTEHVGDRIILGFDHETDAAAASARLPHQIHRSGTQLTITVPDAPRLVPDLLEDLRSAGLPVAEVEVRRPTLDDVFLKLTGRSLREGGATAETTDRDTTLVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVD7</accession>
    <name>U5VVD7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359112.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06460"/>
    <dbReference type="EnsemblBacteria" id="AGZ39581">
        <property type="protein sequence ID" value="AGZ39581"/>
        <property type="gene ID" value="AFR_06460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1310"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119884_1_0_11"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13666" checksum="0F6C52E23B61DDC2" modified="2014-01-22" version="1">MHPFRAAVEARDIEAAIALLSDRVVFNSPIAFTPYEGRQAVGTVLRAVFEVFEDFHYVREIGADDAADHALVFRARIGERQLEGCDFVHRGDDGSIDEFTVMVRPLNGAMALAEAMKAKLAASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVH3</accession>
    <name>U5VVH3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361062.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41003">
        <property type="protein sequence ID" value="AGZ41003"/>
        <property type="gene ID" value="AFR_13585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2758"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_2_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20937" checksum="55326A9C1BFB97AF" modified="2014-01-22" version="1">MARWQPDARQRLQQAALELFTEQGFAATTVPAITERAGLTTRTFFRHFADKREVLFADEAELPAYARKLVAEAPAGTSPLTITLEGLRAVAEARFEGRKDTIRQWRAIVATDAGLRERELQKRAALATAVRDGFCDRGLDPTRAALLAETSATLMYVSVTAWLEADDDRRLYDIIKTALTTLRATLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVP9</accession>
    <name>U5VVP9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">EmrB/QacA subfamily drug resistance transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_07135</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359247.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07135"/>
    <dbReference type="EnsemblBacteria" id="AGZ39716">
        <property type="protein sequence ID" value="AGZ39716"/>
        <property type="gene ID" value="AFR_07135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1456"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_2_3_11"/>
    <dbReference type="OrthoDB" id="1558246at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="35"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="545"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="578" mass="60661" checksum="21D57C13A1131CE1" modified="2014-01-22" version="1">MSNPSTTAPAEAGPAGGPPAGRPSVEFTHRQILTILAGLMMGMFLAALDQTIMATATRTIADDLNGFDLQAWATTAFLITSTISTPLYGKLSDIYGRRGFFLFAIVAFVVGSILCGMSQSMYELAAFRAIQGIGAGGLMSMALAIIGDIVPPRERAKYQGFFLAVFGTASVIGPLLGGFFAGQDSILGITGWRWIFYLNVPIGIAAMIVVARVLHLPHTRTNHRIDWPGAVALIVGLVPLLTVAEQGRTWGWDSPRALACYAIGLIGIVAFILAERAYKDEALLPLRLFKNRTVAVGSISSTIIGMAMFGGLLTVPLYLQIVKGSSATEAGLQMIPFVVGIMAGSITAGQLIARTGRYRIFPIIGSSLMLIALGLFATIGADTPLWKTMLIMVVMGLGLGGNMQPMITAVQNAVSPREIGVATSSVTFFRSMGGTLGTAIFLSVLFNVVGGKINSAYEKAQGTPAFQSAVAADPSQLQTLQRAQGGDALSDTSFLGRLSDVVSHPFKVGFSDGIQLIFLLAAAVMVIGLIVVLFLPEVPLQQRSAQQQRADDAAAAAASDGVPGEVGAGPEVETKSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWH5</accession>
    <name>U5VWH5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_08780</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359576.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08780"/>
    <dbReference type="EnsemblBacteria" id="AGZ40045">
        <property type="protein sequence ID" value="AGZ40045"/>
        <property type="gene ID" value="AFR_08780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1787"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="32996" checksum="87E837423B169A2F" modified="2014-01-22" version="1">MADTAISTEDLVKTYGRGSRGLAGLDLEVRTNEVYGYLGPNGAGKSTTIRILLDLIRPTGGSVRVLGLDPRTDGVALRRRVGYLAGDFVVDGRQTSRQLLAYLGKLRGGVPAARITGLADRLGLDLDRPIRGLSKGNRQKAGIVQAFMHSPELLVLDEPTSGLDPFLQQEFVHLVQEARREGATVFMSSHVMSEVQQTADRVGIIRDGRLVAVESVESLRAHALRSVTLTFDGEFDAAVFDRLPGFTDLSVTGATLHGRLSGHTDELLKAASRYTVLDLLCEEPDLEEIFFHYYALPEAGHVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWH6</accession>
    <name>U5VWH6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cation diffusion facilitator family transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08635</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359547.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08635"/>
    <dbReference type="EnsemblBacteria" id="AGZ40016">
        <property type="protein sequence ID" value="AGZ40016"/>
        <property type="gene ID" value="AFR_08635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1757"/>
    <dbReference type="eggNOG" id="COG1230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_0_0_11"/>
    <dbReference type="OrthoDB" id="948593at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="31538" checksum="87E61C73C487D268" modified="2014-01-22" version="1">MGAGHDHGGQALRAGERHRSRLWWALGLLAAFMLVEAVAALMTGSLALLSDAGHMFTDVLGIGMALAAITAAGRAATDSQRTFGLYRLEVLAALGNALLLTGVAIYVLIEALRRFSDPPDVPAGAMLVVAVGGLIVNIVAFLMLRSGAKESINVRGAYLEVVGDLLGSVGVIIAAGIIAVTGWSYADPIVAVIVALMILPRTFALGRSAVRILVQAAPKHLDVAVVRDRLAAVPGVCDVHDLHVWTLTSGMDVASAHLSLEPAAELGAVLATARETLHEEFDIDHATLQVEPVTAGGSCTPAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWP0</accession>
    <name>U5VWP0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359577.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08785"/>
    <dbReference type="EnsemblBacteria" id="AGZ40046">
        <property type="protein sequence ID" value="AGZ40046"/>
        <property type="gene ID" value="AFR_08785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1788"/>
    <dbReference type="eggNOG" id="COG1510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120349_2_1_11"/>
    <dbReference type="OrthoDB" id="1646730at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="16799" checksum="033D6273E04A7B68" modified="2014-01-22" version="1">MDISAERRYAEEAGVVLAGMGMPLAYGKLLGWLLICTPAAQSGAELSTALGLSKGSVSAGLRTLESSGLVRRVAMPGRRGSFYEMTPDAMTQAAGSEKFTLFRELMDRGLEVIGGEQAPGADRLRTTRDFYAFIEAEMPKLIDRFRTEQRGRSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWS4</accession>
    <name>U5VWS4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15855"/>
    <dbReference type="EnsemblBacteria" id="AGZ41453">
        <property type="protein sequence ID" value="AGZ41453"/>
        <property type="gene ID" value="AFR_15855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3219"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665291" evidence="2">
        <location>
            <begin position="29"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="349" mass="35391" checksum="045CD887C8A021CB" modified="2014-01-22" version="1">MRTLVCPAPTVRSGRILAATAVAILAAATTAGCQKDTAGGSKNAGGTTTYPTKTVQLMVPAAPGGGWDLTARTMQKTMTDGKLTDQAVEVYNVTGAAGTIGLAQLVSKNQGDAHHLMVMGLVMVGGVVANKSPVKLDQVTPIATLTAEQEVIVVPADSKYTTLKQLMDDAKASPASINWGGGSAGGTDQILVGLLAKAAGADPKAMKYVAYSGGGEAKAALLAGDLTAAVSGQSEFKDLVSAGKVRALAVSGATGVDVGGGKPAPTIKEQGLDVELMNWRGVVAPPGINAADKTAVTAFVDKLHSSPDWTSAMQAQGWEDFYKSGDAAAAFFTSENERITTVLTDIGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWT9</accession>
    <name>U5VWT9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome bc1 complex Rieske iron-sulfur subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cytochrome bc1 reductase complex subunit QcrA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Rieske iron-sulfur protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">AFR_09430</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Iron-sulfur subunit of the cytochrome bc1 complex, an essential component of the respiratory electron transport chain required for ATP synthesis. The bc1 complex catalyzes the oxidation of menaquinol and the reduction of cytochrome c in the respiratory chain. The bc1 complex operates through a Q-cycle mechanism that couples electron transfer to generation of the proton gradient that drives ATP synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Rieske iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359795.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09430"/>
    <dbReference type="EnsemblBacteria" id="AGZ40175">
        <property type="protein sequence ID" value="AGZ40175"/>
        <property type="gene ID" value="AFR_09430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1922"/>
    <dbReference type="eggNOG" id="COG0723">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050668_0_0_11"/>
    <dbReference type="OrthoDB" id="1632945at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045603">
        <property type="entry name" value="QcrA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="PTHR10134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19297">
        <property type="entry name" value="QcrA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="15">
        <location>
            <begin position="284"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AGZ40175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="367" mass="39974" checksum="B9DC10A2E136C3FC" modified="2014-01-22" version="1">MTVVKDGAHGAGTEPVDVNDPRLSRFDIVREGARRDDIEIVTYESQFLGTNSKAEKRVVRNIAFLFLLSGAAALVFLVFYIVWPWEFELGATDSDFFTPILGISLGVSLLALGFAILAWAKKLLPHEVSIQDRHNTPSSDEDRLITGQTIAYLGEELGVQRRPLLKGAIGLGLAPLGLVAAAPLIGGLIENPHKDDEPMMYHTGFDPVPNDGNLVRLTREDGTPVRPEDVSVGGQITVFPGIPGGATNHYADSPTLLIHLRSTDAEDARGRNAADATNANSMWGNYIAYSKICTHAGCPASLYEQQTNRLLCPCHQSQFLITDNARPIFGPASRRLPQLPIEVDAEGFFVAASDYKEAIGPDFWERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWZ1</accession>
    <name>U5VWZ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Large, multifunctional secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_15535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361448.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15535"/>
    <dbReference type="EnsemblBacteria" id="AGZ41389">
        <property type="protein sequence ID" value="AGZ41389"/>
        <property type="gene ID" value="AFR_15535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3155"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_302417_0_0_11"/>
    <dbReference type="OrthoDB" id="15684at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010496">
        <property type="entry name" value="3-keto-disaccharide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16990">
        <property type="entry name" value="CBM_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06439">
        <property type="entry name" value="DUF1080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665433" evidence="1">
        <location>
            <begin position="26"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="3">
        <location>
            <begin position="30"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="2">
        <location>
            <begin position="662"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="986" mass="106813" checksum="714355C2C39E681E" modified="2014-01-22" version="1">MVRALVLSLVTAAAAVAISPATAHAAELPPQEPGVTLRTFDTRVPLTAICKLKPGQTPNVDRLTPTIDYRTDADFGLSDNFVAHVLGNLNVPADGAYAFRLTSDDGSRLYLDDAEVIDNDGLHGVVSKDNTVTLTAGVHALRIEYFEAGGGQELSLAWQPPGADAFVPVPTSVLSTDAEVVRVTAPGQKQCEGAFDTPGDGLPLDAVNPGYTLTNLRPEGFQPQVSAMDWLDDGRMVITTWGGSDEVKGEVYIVSGVTGKTGPSKVKYKKIAGGLKEPMGVAVVDDTIYVSQKHELTELQDTNGDEVTDSMRTVATWPFGGNFHEFAFGLLYRGGDFYLNLSVSINLGGATTNPQPVANRGTSLKVNRRTGKVTYVAGGLRTPNGLGWGPQGELFVADNQGGWLPSSKLLHIKQDRFFNHYTNPAGPYDDNAVTKPMLWLPQNEIANSPSTPVTLKKGPFEGQLIFGDVTYGGLQRAYLEKVHGEYQGAVFRHTQGLESGVNRTTIGPDGSIYIGGLGSDGNWGQEGKLRYGLQKLTPNGVTAFDMKSMSATSTGFTVEYTKPLSAETVANIAKGYQVEQWRYAATPAYGGPKVDEKKLTVTAAKVSSDRRKVTLTVDGLERDRVVHLRSPRPFSAAGGEQLWSTEAWYTLNAIPGPEADQVFYEAEEGRRQGTAALATDHPGHSGVGFVAGFGNLNASTTVHTEVSKKGEYEVGLRYSNGPDPFAGNKTVSIHVNGRKIRQTVLPSTVTWNDWATKTERLTLLEGANTIEYRVDALDTGHVNLDLVTVRKPGQRITLFDGKNLTEWQHTDGRTASWPRVAGNAMEVCCGDLRTRQAFGDYKLHVEFKVPLLPPEVTGQNRGNSGVYQQERYELQILDSYGDTTLDNNEAGAIYTQKPPDVNAAKAPEVWQTYDIDFRAARFDAAGAKTENARISVVWNGVTVHKNYEITTVTGGSIAEGPSTGSIRLQDHGNKVQYRNIWIEPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX70</accession>
    <name>U5VX70_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361623.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16330"/>
    <dbReference type="EnsemblBacteria" id="AGZ41548">
        <property type="protein sequence ID" value="AGZ41548"/>
        <property type="gene ID" value="AFR_16330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3312"/>
    <dbReference type="HOGENOM" id="CLU_2327545_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="98" mass="10433" checksum="6736F511D7B42168" modified="2014-01-22" version="1">MDVSEMAYPGREAMRAQVRRQYGNGEALDALQYQFSYWLTNGENTPEAVREPVEGAVAGYFAKAAAVGKWWGIGLGAGAGLSLWAVAAVVVLLVKAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX86</accession>
    <name>U5VX86_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">rpsD</name>
        <name evidence="10" type="ORF">AFR_10255</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="6">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360122.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10255"/>
    <dbReference type="EnsemblBacteria" id="AGZ40340">
        <property type="protein sequence ID" value="AGZ40340"/>
        <property type="gene ID" value="AFR_10255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2089"/>
    <dbReference type="eggNOG" id="COG0522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092403_0_3_11"/>
    <dbReference type="OrthoDB" id="1211060at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_B">
        <property type="entry name" value="Ribosomal_S4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005709">
        <property type="entry name" value="Ribosomal_S4_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="PTHR11831:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01017">
        <property type="entry name" value="rpsD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S4" evidence="9">
        <location>
            <begin position="2"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="S4" evidence="8">
        <location>
            <begin position="91"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="202" mass="23172" checksum="98B95918413F4E95" modified="2014-01-22" version="1">MNNSRPKARLSRALGIPLTRKCVKYFERRPFPPGVHGRGRRKASDYQVRLLEKQRLRHQYNISEAQMRKAYDDAHRKEGKTGENMVQLLESRLDATVQRSGLARTIYQARQLVAHGHFTVDGKKVDRPSYRLKPGQVVEVRESSRQKPPFQIAATGAHLDGPTAPYLSTVLEDLRTTVIRKPLRTEVPVVCDEQLVVEFYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXE9</accession>
    <name>U5VXE9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative amino-acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_10235</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360114.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10235"/>
    <dbReference type="EnsemblBacteria" id="AGZ40336">
        <property type="protein sequence ID" value="AGZ40336"/>
        <property type="gene ID" value="AFR_10235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2085"/>
    <dbReference type="eggNOG" id="COG0531">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_18_1_11"/>
    <dbReference type="OrthoDB" id="527053at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="429" mass="42715" checksum="CF03DC35A692BCFA" modified="2014-01-22" version="1">MTPTGGLSRAQGTALCVGAVLGTGVISMPALAASVAGPASLLAWLALILLSAPLAWTFAALGARYPDGGGVSTYVRLAFGSRAAAAVGWCFYFAVPLGAPPAAAFAGGYVADVLGGGRATVIATFVVIMAVVFVLNWFGLRISGRVQLYLAATLAVLLVITVVAALPHARLANLTPFAPHGWAGVGAAAAILVWGFAGWEAVSSLSGEYRNPRRDVPRATAAAVLIVGVLYLAVAATSVLVLGPAAGSSAAPLADLLAVGVGGPVRPLTAVVAVLLTIGAVNAYLAGASRLGVALTRDGALPARFVGTPRRSLAFVTLGGLASVLLPLDLHTAMLLVTGCFTLVYVLGTAAALKLLPAGWSRRGAGVAFVAVLGLLVLNGLPALLSLAVAAGAVLYVSLRTAPKIASPSSSSAGSIAPNPNTNPVESLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXR8</accession>
    <name>U5VXR8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ABC transporter permease protein yurN</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_11205</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360488.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11205"/>
    <dbReference type="EnsemblBacteria" id="AGZ40530">
        <property type="protein sequence ID" value="AGZ40530"/>
        <property type="gene ID" value="AFR_11205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2282"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_0_11"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="67"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="33463" checksum="F4958E9DD3C4A720" modified="2014-01-22" version="1">MKHGRYPFIIGFLIVPLAIYGTFVVAAYLQAFQLSFTNWRGFSPDVNYIGFDNFVKLFSDDTFWKAIRHHIVLLIFLPLITIILALFFAFLLNVGGGSKGGRTRGVWGSKFYRVVFFFPQLLALALVAVIFARVYSPDDSGLINGLLNQIGSGPILFLADKNLALWSILAVLVWQAVGFYVVLFSAGMGSIPTEIYEAAALDGATRGTLFFKVTLPLLWNTLQVAWVYLGIAAFDAFALVNVMSVDRGGPDGATTVLGLEIFRNMQDYAQFGYASAMGVVLFFLTITFAALTLRVTRRDAIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYE3</accession>
    <name>U5VYE3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cellulose 1,4-beta-cellobiosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12220"/>
    <dbReference type="EnsemblBacteria" id="AGZ40730">
        <property type="protein sequence ID" value="AGZ40730"/>
        <property type="gene ID" value="AFR_12220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2484"/>
    <dbReference type="HOGENOM" id="CLU_720858_0_0_11"/>
    <dbReference type="OrthoDB" id="2116563at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="383" mass="40783" checksum="7AC330E8A662AC98" modified="2014-01-22" version="1">MAAFCTELRHLTQRCSLSATAASRRLHISRSQYYAVINGEVRRPPDWAKVEVILRMCEQPDTAIGEWRQRHDEMVFGYDRLRTRRRAVPPADAAPDSAAAVVPAPESSPAPAPEIDLARVAGRGAEPTADDEPAEPDDRPARRASWRRVLTVAAVVVLLAAVGGAAARIAVSGVPGDAGGLRPSTGPSTTATVLPALAGDGTLGAPLPGAAPETGNPADPDERKACVSDAPPPGTELLTVPRRHRPGNNKMNNDWWGSTEQVSFNAANPAAFDANVAAGGEHVWDVIILHSCVPLVAGHRYLLRFTAATNPAGPVMMRVQDSVQPEASESVTQTLDFTPRAAARTISFTAKHTSRSSEVTFQVGDWEGPFRLRVIDISLTETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYI6</accession>
    <name>U5VYI6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_18380</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362327.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18380"/>
    <dbReference type="EnsemblBacteria" id="AGZ41954">
        <property type="protein sequence ID" value="AGZ41954"/>
        <property type="gene ID" value="AFR_18380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3730"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_9_11"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="28"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="340"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="373"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ41954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="580" mass="61244" checksum="2883EA3E65C95A76" modified="2014-01-22" version="1">MSALPVADRRTVAREIRALQRRHRGRLAGIVALQVVTVAVGLLPPILLGRLVDGITGGFDTHDADLTAVLIAACLLAHGVLAFVATRAAFTLGELVFAELREDFTDRILALPLGTVEETDSGEILSRTTTDMEAIREIVRTGVPETLIGVLTTVLTVVAAFLIDPLIAGGVLIGLPIIALATRWYTRRAPAAFAARFATRARLTSAVTETARGADTVEAFDLAGDRRDLVRERIGTARDAALVPIGLHVRWFPAVQIGYHLPLLVVLAWGAVLVQQGRAEIGAVAAITLLVRALITPLDDLAYWFGELQSATAAFARIFGVPRAPASPAFTPVPSLDARVEVAGVTFGYAPDRPVLHDVTLVVEPGERVVIVGASGAGKSTLALLIAGVHTAATGRVAIGGTPVAHLPDGLLRDRVALVTQEDHVFTGTVADNLLLARPEASPEELVRALEAAGATWVTDLGAELGADAYLPTPAETQQLAAARLILRAPQVLVLDEATSALSRAQAARLETSLAETAGRRTIIEVAHRLDTATRADRVVMLVDGRIAESGAHQDLLTADGPYARLWQAWLGGQARTVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ77</accession>
    <name>U5VZ77_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18865</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglycosylase family. Rpf subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362420.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18865"/>
    <dbReference type="EnsemblBacteria" id="AGZ42047">
        <property type="protein sequence ID" value="AGZ42047"/>
        <property type="gene ID" value="AFR_18865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3832"/>
    <dbReference type="eggNOG" id="COG1652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045108_0_1_11"/>
    <dbReference type="OrthoDB" id="2063919at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13925">
        <property type="entry name" value="RPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010618">
        <property type="entry name" value="RPF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06737">
        <property type="entry name" value="Transglycosylas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666228" evidence="3">
        <location>
            <begin position="32"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="4">
        <location>
            <begin position="132"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="181" mass="18771" checksum="46BAD1D71616BFBC" modified="2014-01-22" version="1">MAQNHQRFLRALGLMAAGAAGTVAMLGPATAAEAKSGVNWDAVAKCESGGNWKINTGNGYYGGLQFSRSTWKAYGGGKYASTANKASKAEQIRIAEKVLDGQGIGAWPTCGKKGKTKAKSSSSSAHSSARTSHYTVKPGDTLASIAAAKHVKGGWRALFKHNRSVLGGNPNVIVPGQRLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZC3</accession>
    <name>U5VZC3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MerR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362601.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19785"/>
    <dbReference type="EnsemblBacteria" id="AGZ42229">
        <property type="protein sequence ID" value="AGZ42229"/>
        <property type="gene ID" value="AFR_19785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4015"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052806_1_0_11"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="32868" checksum="7756DDAB04D906FF" modified="2014-01-22" version="1">MDTNTGLLTIGQLARRTGLAARTLRFWSNEGAITPVGRTAGGYRLYDAASVARVELVRTLRELGLGLDDVRRVLDGRATVAEVAEVQVAAIDAQIRSLKVSRAVLSTVSKQRSTTEETALMNRLARLSAAERLQIIDDFKADVFGRLDLEPGLRDRINDLRIDLPDDPTPDQVDAWIELAGLVQDPSFRARMRTFLTLNTPVPGQKAPPGANIWWARQVAETVAAARENGVGPGEPAAAEVITAMFGDADLSAVLVSLEAGIDAGAEQYRRLVARVRGQRSHPDATEELRWLATALRLATAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZE5</accession>
    <name>U5VZE5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Epoxide hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362509.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19325"/>
    <dbReference type="EnsemblBacteria" id="AGZ42137">
        <property type="protein sequence ID" value="AGZ42137"/>
        <property type="gene ID" value="AFR_19325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3924"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_2_11"/>
    <dbReference type="OrthoDB" id="1439833at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="28"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="34183" checksum="1265BC1C9B15E3A7" modified="2014-01-22" version="1">MNFPQPTLVSVNGVELEVFEAGRQNAGKPIVLCHGWPEHAFSWRHQLPALVAAGYHVIVPNQRGYGNSSRPEEVTEYDIEKLSGDLVALLDHYGYDDATFVGHDWGAMVVWGLALLHPHRVNKVINLSLPYQERGERPWIEVMEQFLGGDFYFVHFNRRPGVADAVLDKNTFQFLRNLYRKAEIGTSMVDLAEAETPAGDPLLSDSELAVFVSAFEKSGFTGSINWYRNLDRNWHLLADVDPIIRQPTLMIYGDRDAIRKSENLTDFVPGVEVVSLDCGHWIQQEKPEETTEAILRWLDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0C1</accession>
    <name>U5W0C1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21965</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363034.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21965"/>
    <dbReference type="EnsemblBacteria" id="AGZ42663">
        <property type="protein sequence ID" value="AGZ42663"/>
        <property type="gene ID" value="AFR_21965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4453"/>
    <dbReference type="HOGENOM" id="CLU_1640166_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17960" checksum="AB24B8F9F94FDFFB" modified="2014-01-22" version="1">MVSIIEAVEVMREDHPRIDVGGLYQQFRCRGGRWALITREDVRIALAGLECAARQEFPVPHPQRIVPRTPEGAWPQFQRAVTDLHTTGGLDAVEITRHLIDTGWSQVAEADVTAVLRRIRTGNTGRHFTPGRSLPAPRRTVPEICPSCDVRITDLGTCRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0H6</accession>
    <name>U5W0H6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkanesulfonate monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363094.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22265"/>
    <dbReference type="EnsemblBacteria" id="AGZ42723">
        <property type="protein sequence ID" value="AGZ42723"/>
        <property type="gene ID" value="AFR_22265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4513"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_1_4_11"/>
    <dbReference type="OrthoDB" id="919913at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="361" mass="39519" checksum="89195BCDADC19F51" modified="2014-01-22" version="1">MPVEFISAISPSAGFGMGTAFDTAHARKYAHALDDGGFDYTLVAYHSSAVDANQLAQFVVNHTERIKPMLAHRPGVIFPTHAARTFATLDQISRGRLAIHIISGGSDEEQRREGDYLTKTQRYERSDEYIQLLRQAWTATEPFSHDGSYYKFEGFHSDVRPYDGTIPISVGGSSEDAYRVGGQRGDIFGLWGEPLAETKQQIEAVGKYADAAGRPRPRIWVSFRPIVAATDELAWEKAHRILGQVQAAAPVPASARHYPLTERPANVGSQRLLDVAARGELHDRALWTPLVTATNAAGSSTALVGSPETVAAALLDYVDIGCELLSIRGYDPLNDAIDYARYVLPIVRQELSRRTPLAVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1L1</accession>
    <name>U5W1L1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362203.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17935"/>
    <dbReference type="EnsemblBacteria" id="AGZ41865">
        <property type="protein sequence ID" value="AGZ41865"/>
        <property type="gene ID" value="AFR_17935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3638"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665974" evidence="1">
        <location>
            <begin position="28"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="23467" checksum="CE8B571225CB3B2F" modified="2014-01-22" version="1">MTLLSSSRVVVVGGTSGIGLAVARAAAAHARVTIGSRSEESVKRALAELPDTVQGRAVDVTSPASLESFFAAAGPFDHLVYTAGDALVRGPIGDYDARQARDFFDVRLFSALTTVRLALPTLNASGSVTLTSGAAAYHGGAGRLLGSTVSAAVIAAARSLAIELAPIRVNAVAPGVVRTPLWADLPGDARERLFASAGAVDAEDVAKAYLGFLDQDHATGNVAVVEGRSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1V6</accession>
    <name>U5W1V6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18460"/>
    <dbReference type="EnsemblBacteria" id="AGZ41970">
        <property type="protein sequence ID" value="AGZ41970"/>
        <property type="gene ID" value="AFR_18460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3746"/>
    <dbReference type="eggNOG" id="COG0586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="29784" checksum="3400C159F825CDB6" modified="2014-01-22" version="1">MDTTLALFDRTAVLAAETSTEQSGLVSFVTDLVEKLGGPGAGLAVALENLFPPIPSEVILPLAGFAASRGDLSLASAIIWTTIGSVVGAVALYYVGALLGRDRVRRIVERLPLVKLADVDRTEAWFAKHGVKAVFFGRMIPIFRSLISIPAGLERMKLGTFLLFTTLGSLIWNSIFIFAGYGLGENWTEIETWVGTYQNIVIAVCALIFGWFVVNRIVKARRRRAGETPADGEGFLPAVDPAPSHQDLRSHSQQEPEAGPGRVYGSPRQYPEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W201</accession>
    <name>U5W201_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative nitrate extrusion protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_18695</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362388.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18695"/>
    <dbReference type="EnsemblBacteria" id="AGZ42015">
        <property type="protein sequence ID" value="AGZ42015"/>
        <property type="gene ID" value="AFR_18695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3795"/>
    <dbReference type="eggNOG" id="COG2223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033198_0_1_11"/>
    <dbReference type="OrthoDB" id="1217595at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17341">
        <property type="entry name" value="MFS_NRT2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="PTHR23515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="245"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="445" mass="47229" checksum="C65A9D6F7787650A" modified="2014-01-22" version="1">MSATTLERPAALTVQGHWIDDWRPEDPTFWEQTGARIARRNLIFSIFSEHIGFSVWTLWSVLVLFLGPAYGIDPAGKFLLTAVPALVGAALRIPYTFAVARFGGRNWTIISASLLLIPAVLAAFLIEPGVSYSTLIVLAAVAGVGGGNFASSMANINAYYPDRLKGWALGINAGGGNLGVAAVQLIGLFVLAVFGAGSPGLVAGIYIPLIVIAAAGSALYMDNLSQARNEKRGMRDATREPHTWIMSLLYIGTFGSFIGFGFAFGQVLQVQFADVFSTPVKAAYLTFLGPLLGSLIRPVGGALADRLGGARVTFWNFVAMAAGASIVLLASQLRSLPLYLVGFIALFVFSGIGNGSTYKMIPRIFALKHPGDNHEARRLSGAVIGIAGAIGAFGGVLVNLAFRQSFLTYKTADAAYIAFIAFYALCVLVTWAVYLRSSSRKLAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W268</accession>
    <name>U5W268_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transglutaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24790"/>
    <dbReference type="EnsemblBacteria" id="AGZ43224">
        <property type="protein sequence ID" value="AGZ43224"/>
        <property type="gene ID" value="AFR_24790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5022"/>
    <dbReference type="eggNOG" id="COG1305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008973_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013589">
        <property type="entry name" value="Bac_transglu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08379">
        <property type="entry name" value="Bact_transglu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="167"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="276" mass="29426" checksum="D2A8B9E21E51866E" modified="2014-01-22" version="1">MDLDGARRISYRVEQTFRYAYEAPVTALAQRLVVVPRRRHGDLYRRHHRLEVTGAAHARRARLDSAGNTVVRVVAARVEHAVEFRLTADLDRVAGDGPPELPFAALRDPRLLRPTRLTAPDEALRDLAAPCTGTDAEDTASQVCAAAHAALTYRYGVTTVSTTAAQALAAGQGVCQDSAHVMLAMCRLLGVPSRYVSGHLIGQGGTHAWVEILVPRGDRAVALAFDPCHGRRAGNGYLTVAVGRDYADVAPTSGTYVGAPGGTLTGTRNVTVTAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W275</accession>
    <name>U5W275_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363697.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24195"/>
    <dbReference type="EnsemblBacteria" id="AGZ43107">
        <property type="protein sequence ID" value="AGZ43107"/>
        <property type="gene ID" value="AFR_24195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4901"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="146"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23476" checksum="752D58A3B3EA7EB4" modified="2014-01-22" version="1">MPSYRVILAEDSVLLREGLVSLLTRFGHEVVAAVGDAPALHAAVSADRPDIVVTDVRMPPGHQDEGLRAAVELRGTDPALPILVLSQYVETTYATQLLDSGDGTGVGYLLKDRIGRVQEFLEALDRVVLGGTVVDPEVVRQLLRRRRDPLAPLTPREREVLALIAEGRSNAAVAARLVISDAAVAKHIRGILQKLDLPQDGEDHRRVLAVLAFLRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2K8</accession>
    <name>U5W2K8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular solute-binding protein family 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362513.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19345"/>
    <dbReference type="EnsemblBacteria" id="AGZ42141">
        <property type="protein sequence ID" value="AGZ42141"/>
        <property type="gene ID" value="AFR_19345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3927"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_12_3_11"/>
    <dbReference type="OrthoDB" id="480174at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666316" evidence="1">
        <location>
            <begin position="20"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="437" mass="45620" checksum="6809CDB41D479D83" modified="2014-01-22" version="1">MRFRSLPLALLAATTLALSACSAGSLGSSDDEGGKVELSFLVDNSDSSLKPVEQVAKDFSAKNPGITVKVETRPGGTEGDNIIKTRLSTGDMTDVFLYNSGSLFQAIAPQQNLVPLSDQPYAGQLDDNFKSTVTAGDQLYGVPLGGVTGGAVLYNKKVFADLGLKVPATWAEFMANNAKIKAAGVAPVIQTFGDTWTSQLFVLGDFHNVSAADPQFAANYTANKVKFASTPAAVAGFQHQQEVHDAGYLNKDFASAKLDDGLKQLAQGKGAQYPMLTFAVGGMVANFPESAKDVGLFALPGADAAKNGLTVWSPAGIYIPKSTEGAELEAAKKLLAYIASPDGCASQAAAYAPSGPFLVKGCTLPADVPQAIKDMQPYIDTPGKSSLALEFLSPVKGPSLEQITVEVGSGIRRAPAGAALYDEDVKKQAQQLGLEGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2P2</accession>
    <name>U5W2P2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, TetR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_19890</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362622.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42250">
        <property type="protein sequence ID" value="AGZ42250"/>
        <property type="gene ID" value="AFR_19890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4036"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_28_2_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="20265" checksum="54159BC4C8FA1409" modified="2014-01-22" version="1">MARVSQAQAQENRDRVVETAARLFRERGVQGVSVADLMAEAGLTHGGFYKQFSSKEALVAEATGRAFGELGALLTGLDEQHPDDHAAARSALLDYYFSAEHRDHAGKGCPTTALGPDVAREAPGSPAREPYAEGVEGFARWMGDESEENLVAIATMAGALMLSRATAGSELSDRILAAARRSLSSGDGRSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Q0</accession>
    <name>U5W2Q0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Poly(ethylene terephthalate) hydrolase</fullName>
            <ecNumber evidence="7">3.1.1.101</ecNumber>
            <ecNumber evidence="3">3.1.1.74</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_19940</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(ethylene terephthalate)(n) + H2O = (ethylene terephthalate)(n-1) + 4-[(2-hydroxyethoxy)carbonyl]benzoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49528"/>
            <dbReference type="Rhea" id="RHEA-COMP:12420"/>
            <dbReference type="Rhea" id="RHEA-COMP:12421"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:131701"/>
            <dbReference type="ChEBI" id="CHEBI:131704"/>
            <dbReference type="EC" id="3.1.1.101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:49529"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47348"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:50477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:47349"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.101" evidence="7"/>
    <dbReference type="EC" id="3.1.1.74" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19940"/>
    <dbReference type="ESTHER" id="9actn-u5w2q0">
        <property type="family name" value="Chlorophyllase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42260">
        <property type="protein sequence ID" value="AGZ42260"/>
        <property type="gene ID" value="AFR_19940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4046"/>
    <dbReference type="eggNOG" id="COG4188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050214_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041127">
        <property type="entry name" value="Chlorophyllase2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12740">
        <property type="entry name" value="Chlorophyllase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666321" description="Poly(ethylene terephthalate) hydrolase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ42260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="320" mass="32705" checksum="B7FA84CA74C6787F" modified="2014-01-22" version="1">MGLAATAGTGALAACSAETATAPTATPSSAVPILASTTASSLPPQDVSAPYTPPPGRAPGRAHAVGRQVLQFQREDRSLPTTVWYPAEGPVPATPEPADDAAPARGRFPLVLFSHGLTAQPGDYAAMLARWAQAGFVVAGPTYPRTSYGAADFYALDIVNQPADASFVIDSLLATTGPLRAIIDTDRIAAAGHSAGGITTAGLFSAHRDERLKAGMVLAGTDFQGAAFTGPPAAMLFVHGRKDNTVAYRAGHAVFAAVPWSRAMLTITDGGHVTGAADFEATTGTSTEFFRWALYGDAAAKARLPQAAATGEVATLESGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2S2</accession>
    <name>U5W2S2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364317.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26330"/>
    <dbReference type="EnsemblBacteria" id="AGZ43528">
        <property type="protein sequence ID" value="AGZ43528"/>
        <property type="gene ID" value="AFR_26330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5337"/>
    <dbReference type="HOGENOM" id="CLU_2875570_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665488" evidence="1">
        <location>
            <begin position="20"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="65" mass="6493" checksum="BA8B55762A05A4F1" modified="2014-01-22" version="1">MSIGSLSSGTVSTATLASADASANAAKARETEAAPTIADHVALADEQEPIRSVSSTLGTLVDTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W356</accession>
    <name>U5W356_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362738.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20470"/>
    <dbReference type="EnsemblBacteria" id="AGZ42366">
        <property type="protein sequence ID" value="AGZ42366"/>
        <property type="gene ID" value="AFR_20470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4151"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_8_5_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023934">
        <property type="entry name" value="LLM_FMN-dep_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04036">
        <property type="entry name" value="LLM_CE1758_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="367" mass="41044" checksum="41ED36F10507378C" modified="2014-01-22" version="1">MQFGIFTVGDVTPDPTNGRKPTEHERIKAMVAVAKKAEEVGLDVFALGEHHNEPFVPSSPTTMLGYIAAQTEKLLLSTSTTLITTNDPVKIAEDYAMLQHLADGRVDLMMGRGNTGPVYPWFGKDIRAGIPLAIENYALLHRLWREHTVDWKGKYRTPLQSFTSTPRPLDEIPPFVWHGSIRSPEIAEQAAFYGDGFFANHIFWPASHTQRMVALYRERYAHYGHGSADQAIVGLGGQVFMRKNSQDAVKEFRPYFDNAPVYGHGPTMEDFTRETPLTVGSPQQVIERTLGFRDYVGDYQRQLFLLDHAGLPLKTVLEQLDLLGEEVVPVLRKEFAALKPAHVPEAPTHASLLAAAQDQDLKEKVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3E9</accession>
    <name>U5W3E9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364535.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26890"/>
    <dbReference type="EnsemblBacteria" id="AGZ43639">
        <property type="protein sequence ID" value="AGZ43639"/>
        <property type="gene ID" value="AFR_26890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5449"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_5_5_11"/>
    <dbReference type="OrthoDB" id="1442962at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="19859" checksum="C4510AFCAB17A7FF" modified="2014-01-22" version="1">MTTLQDRPNTALVIVDVQNRVVASTHERDAVVANIGSLVSKARAENVPVVWVQQFDDVLVKGTEPWQVVPELSPEDADQHIDKAWADSFEETELETVLAGLGVGRLIVTGAQTDECIRSTLHGALVRGYDTILVSDAHTTEDLSEWGAPPPDKVIAHTNLYWDGHKAPGRVAGTVETKNVSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W440</accession>
    <name>U5W440_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363047.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22030"/>
    <dbReference type="EnsemblBacteria" id="AGZ42676">
        <property type="protein sequence ID" value="AGZ42676"/>
        <property type="gene ID" value="AFR_22030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4465"/>
    <dbReference type="HOGENOM" id="CLU_2930639_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="60" mass="6597" checksum="93C631DD16FD2086" modified="2014-01-22" version="1">MRTAPVIRSLLATTYLLALVLHGRWNSLSAVLAASVVAVWAFPLIRDARLRRGTEPLSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4A5</accession>
    <name>U5W4A5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_22725</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + 2 NAD(+) + UDP-alpha-D-glucose = 3 H(+) + 2 NADH + UDP-alpha-D-glucuronate</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363300.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22725"/>
    <dbReference type="EnsemblBacteria" id="AGZ42815">
        <property type="protein sequence ID" value="AGZ42815"/>
        <property type="gene ID" value="AFR_22725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4606"/>
    <dbReference type="eggNOG" id="COG1004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_2_11"/>
    <dbReference type="OrthoDB" id="647136at2"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="11">
        <location>
            <begin position="320"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="153"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="253"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ42815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="437" mass="46670" checksum="6C2ABA7A98FEF8E8" modified="2014-01-22" version="1">MNLTVIGTGYLGATHAICMAVMGYDVIGVDVDAAKVERLNSGEVPFFEPGLPELLTKALESGRLRFTTSFQEAGEFGDVHFICVGTPQRKGSEAADMTYVDASVTELSKHLHRRALVVGKSTVPVGTAARLAELIRTTAPAGDQVELAWNPEFLREGFAVDDTMKPDRMVFGVTSAWSEEQLRAAFAPVLAQGVPVKVTSLQTAELVKVAANSFLATKISYINAMAEVCEATGADVHDLAEALAYDERIGGKFLRPGLGFGGGCLPKDIRAFAHRAEELGVGQAVGFLREIDGINGRRRARTVDLVVELCGGDVAGKKIAALGAAFKPNSDDIRDAPALDVASTLASMGADVHVFDPAAMENARKAHPELNYGTSALDVARDADVVVLLTEWTEFREIAPEAMAEVVGAQRIFDGRHALDSDAWRAAGWEYKALGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4F8</accession>
    <name>U5W4F8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363386.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23025"/>
    <dbReference type="EnsemblBacteria" id="AGZ42875">
        <property type="protein sequence ID" value="AGZ42875"/>
        <property type="gene ID" value="AFR_23025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4666"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="240" mass="24095" checksum="D87062D3B878D753" modified="2014-01-22" version="1">MTGSLLGQTVVVLGGSAGIGLATARAATAEGAGVVITGRTGSRLEEAAKDVGAVGHAAFDATDSGRLGTFFRTLPGPVNHVLVSGSGPMYAPLAELDTAAAARHVGDHLKLYLDVAQLAATAVQPGGTVLFISGTGGRRPAVGLSVISLLTAGMPALVKNLALELAPVRVNLIAPGFVDTPLSASLLGDGLDARRAQLRETLPIRRVVGPDDVAAVAVHLMVNTALTGATFDVDGGQQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W540</accession>
    <name>U5W540_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">AMP-dependent synthetase and ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363675.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24085"/>
    <dbReference type="EnsemblBacteria" id="AGZ43085">
        <property type="protein sequence ID" value="AGZ43085"/>
        <property type="gene ID" value="AFR_24085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4879"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076053_1_0_11"/>
    <dbReference type="OrthoDB" id="1389630at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="26750" checksum="F840559C5AB9EFB7" modified="2014-01-22" version="1">MPSSTTADLSVLLPGTDRPLLTYYDDATGERTELSATDLGGWAARTAGLLRDDCGLQPGAHVGILLPPHWQTAAILLGAWSSGIAISFRSWARAGLQPAPERPLDAVFVSRKRLDSWLEEVPPSRHRYVLSLAADGARPDDVPAGYLDFVTEVLRHPETTPAYAAVTPDREASTDGTSYAEWGEIAHSIAASLTLRPGDRLLVDASEHEEPVQWLLAPLAAGASIVLCANLDRDRLDARIASEGVTRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W583</accession>
    <name>U5W583_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Guanylate kinase</fullName>
            <ecNumber evidence="2 9">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gmk</name>
        <name evidence="11" type="ORF">AFR_30590</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for recycling GMP and indirectly, cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + GMP = ADP + GDP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2 9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560714.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30590"/>
    <dbReference type="EnsemblBacteria" id="AGZ44378">
        <property type="protein sequence ID" value="AGZ44378"/>
        <property type="gene ID" value="AFR_30590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6193"/>
    <dbReference type="eggNOG" id="COG0194">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001715_1_2_11"/>
    <dbReference type="OrthoDB" id="1502854at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:guanylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="10">
        <location>
            <begin position="9"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ44378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="22572" checksum="4611ED5029A92583" modified="2014-01-22" version="1">MDDDARPAARLTVLSGPSGVGKDSVIELIRARSPWIKLSVSVTTRKKRDYETDQEHYHFVTRSEFQRLIDGDQLLEWAEFAGNLYGTPRAQVEGWLQQGRPVLLKIDLQGARQVRATMPDAQLIFLAPPSVEELKRRLIGRGTDDEETIRRRLEHADEELAHEKEFDRTVVNDFVERAADELVGLLGSSFLTPAQPQPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5A4</accession>
    <name>U5W5A4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560529.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29655"/>
    <dbReference type="EnsemblBacteria" id="AGZ44192">
        <property type="protein sequence ID" value="AGZ44192"/>
        <property type="gene ID" value="AFR_29655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6011"/>
    <dbReference type="eggNOG" id="ENOG5030CXQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_945369_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="294" mass="33331" checksum="30C79C3A10593C45" modified="2014-01-22" version="1">MSQSTVHARVRVRNPAEFLDLDRVLGARLVRADDLPIDEPHTVYCLESQRDGVCCTAEEWQRWTDAGARCLDEISAAYVALLRDHGSPDAQIDLKTGSTSLTWARQPAQWPGRAGRLARRSREAQQRFLARVRAADATYDPVRTEIERRLAEHQSEQRALRARLEREAEQRRVRQELRASVIKEVAQQRVWLYAPGDDDGPVVWVWRRDVTPDPAAPVAAHSAAQDAYQLEKTLLRLHRTPGRRILWDAAARAAVERECAERESTLTFTDWWAALTGSGWRQVSAPRVPASGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Q9</accession>
    <name>U5W5Q9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363918.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25295"/>
    <dbReference type="EnsemblBacteria" id="AGZ43325">
        <property type="protein sequence ID" value="AGZ43325"/>
        <property type="gene ID" value="AFR_25295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5126"/>
    <dbReference type="eggNOG" id="ENOG502ZPRT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092692_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666114" evidence="2">
        <location>
            <begin position="35"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="193" mass="20013" checksum="539A63F1461394A6" modified="2014-01-22" version="1">MRRDWRDTVHVATDLALLGLLVAVAALPLVTAGAALATASAAIQHHLTHGSWPPAHRSWVVFRRALLPGLLAGLATAAVVTLLIADFLAVRHGAVPGGTPLLVLTAVLVAVVCGYAGLVLVAIGTDPDRPWRTTTRGVPLTRALPAATGVIVLAAVLATLVHPVLVPVLGGYTLFALHVIARRPAQRRKILAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W608</accession>
    <name>U5W608_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative RNA polymerase sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_30935</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560782.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30935"/>
    <dbReference type="EnsemblBacteria" id="AGZ44447">
        <property type="protein sequence ID" value="AGZ44447"/>
        <property type="gene ID" value="AFR_30935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6262"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_1_11"/>
    <dbReference type="OrthoDB" id="1857862at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="26"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r3" evidence="5">
        <location>
            <begin position="109"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="7">
        <location>
            <begin position="191"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="300" mass="32557" checksum="15B0085F18F2A9AA" modified="2014-01-22" version="1">MTVTTELPATIGITHDAPSARDLEDLVREHMPMVGHLVRELLNRVPGHVHADDLSSAGFAALLGAARSFDVSRGIPFHRFAAVRIRGALLDELRGQDWASRSVRARARRTATARQELTAALGRTPSEAEVAEMLGIGVAELASVEDDVQKAALLSLQGFPTGAAEEMVPELSEGPEDLLLKRERLGYLHQAIQALPERLRLVVTQSFLQEQPLSDVAAELGVTESRISQLRTEALRLLREGLNSSLAPELLTVAAGGPRTRKGCLQRRRTEYFARVAEQGNLRTRLALTDSHGVPIAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W664</accession>
    <name>U5W664_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ricin B-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_31185</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560831.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31185"/>
    <dbReference type="EnsemblBacteria" id="AGZ44497">
        <property type="protein sequence ID" value="AGZ44497"/>
        <property type="gene ID" value="AFR_31185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6312"/>
    <dbReference type="eggNOG" id="COG3507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_368291_0_0_11"/>
    <dbReference type="OrthoDB" id="1448835at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14200">
        <property type="entry name" value="RicinB_lectin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="5">
        <location>
            <begin position="596"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="420"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="734" mass="76233" checksum="E6D665AF7A05396B" modified="2014-01-22" version="1">MSTHRRPAPRAEPLPVKRRAVPERGRRRASPRPHRLLPVLLGVAVLGIGGVVGPSVVGGAWSPDDKGQDRERITYASLPDDDPGQGLVYDGLVPASADSLCAGIYELDEETCTHGPDPAPAGLKVSRDVPPVTAKVPEPEEPRREAAAVPPDAEIVRDQGGSSLSPGAPALIPDAAPGEADFIMGTHDVACEGDGRTGKRVQVLYLHEFGTPSRYTDFLGSMRTWSAGVDQIFDASAAETGGSRHVRFVTTPQCRVDVAEVQVPEAALESFTGNIDALQTLGYNRTDRKYLIFADSNVYCGIGTFVADNRPGLGNRNNGGPSYGRVDAGCWSSVVAALEVTQMFGALLQDSPNSTGSGSCTDDYDLLCAKDRSGTALRTVCPKKHENRLDCGHDDYFNTNPSPGSYLTKSWNVAQSEFLLRGDGGDDLPDAPNAPPAEPDATPRATPSTEAAEVPAPGPTGGNESDGGGDAVAPPGPDATEPAAPPTTGAPAADPTGEPTAPVPTQQAAAPAVQAVLEVREPTSTSVRLTWSAASPKATYEVEVDGVKIATTVATRARLIGLRPDAKYQVTIRSTATGYVAKGTAQSAPAARPAANAWFVLTNSLTGGAADLYAARTANGTPVTLGGVDGDSQQQWKLVPAGGTSFSLQSRATGKCVVPLDGNPVAGAPLVQGDCTRADSGRWSLFATDHGFSLRTTAGGLVAGVGSQRFGAARLLVLQQPDKARHQSWTAVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6H0</accession>
    <name>U5W6H0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fe(3+)-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_32750</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561146.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32750"/>
    <dbReference type="EnsemblBacteria" id="AGZ44808">
        <property type="protein sequence ID" value="AGZ44808"/>
        <property type="gene ID" value="AFR_32750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6628"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="10"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="25918" checksum="67E4C9BDEC2EB2FC" modified="2014-01-22" version="1">MTTAVEAPAVRARTITRRYADRTVLHELDLEIRRGEFVALIGKSGCGKTTLLRTLAGLDRLDGGDLTVPSSSTVVFQEHRLLPWEKVWRNVALNLPRATARSRAQSALEEVGLGDHADVWPVTLSGGEAQRVALARALVREPELLLLDEPFAALDALTRIRMHGLVRDLIARHRPAVLLVTHDVDEAILLADRVLTMRDGRIAADHPVTGPARHRGDPAFVSLRAELLSELGVVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6J8</accession>
    <name>U5W6J8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3291 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364449.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26665"/>
    <dbReference type="EnsemblBacteria" id="AGZ43595">
        <property type="protein sequence ID" value="AGZ43595"/>
        <property type="gene ID" value="AFR_26665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5404"/>
    <dbReference type="eggNOG" id="COG2329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119287_0_0_11"/>
    <dbReference type="OrthoDB" id="1241950at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021708">
        <property type="entry name" value="DUF3291"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11695">
        <property type="entry name" value="DUF3291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3291" evidence="1">
        <location>
            <begin position="6"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="16962" checksum="083777DCCCCD8683" modified="2014-01-22" version="1">MADHHLAQLNVARLRAPLDSPVLADFVALLPEVNALAESSPGYVWRLQDESGDATALRPFGADLIVNLTVWESPESLRGFVYRTAHLEPMRRRREWFVPLDQPHLVLWWIPAGHVPDLAEAADRLERLRRNGPSGEAFTLREPHPAPGALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6K8</accession>
    <name>U5W6K8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoesterase pa-phosphatase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_32655</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32655"/>
    <dbReference type="EnsemblBacteria" id="AGZ44789">
        <property type="protein sequence ID" value="AGZ44789"/>
        <property type="gene ID" value="AFR_32655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6608"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_527661_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="420"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="3">
        <location>
            <begin position="108"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="3">
        <location>
            <begin position="353"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="500" mass="54318" checksum="287B3FD91676CC72" modified="2014-01-22" version="1">MSTDDGRVHRKVGWAPVRHFTERSLLGLLVVVAVGLGFGLLLLLVRFRWSPLQGLDRSVADGLNGLVAPHEPLVKVLESISQLGGRPVMIWLVAVAVVLLLIRRRTRLAVYLVITGVGALLLDPSLKTLVGRLRPVVDVPVATAPGNSFPSGHALGSMVVYGGLLLVFLPAIAPKWRKPAIGLIAVIIAAVGFTRISLGVHFLSDVLAGWLLGAAWLGVTAYAFRVWRREVGRPVGPIQEGLEPEAAHDVTPAPDEEKVLPHPWTGAAELIVGWVLTFGVLYVIGWAVTNHTNGTWFGNADVGVNRWLQTFRTPALDDLSYGWSKAGDTHMILLVSLVFCPLVLALWRRWRPVLFVVLAMFGELSLFLSTAAAVGRPRPPVDQLDGQMPTSSFPSGHIAATMCLYVSIAVLTVPRVRAWWRWVFVALAVIMPAGVALSRMYRGMHHPTDVFGAGVLTACWITLLWFVLKPNADVEQGNRADAGELTENDADRDLTEAPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Q8</accession>
    <name>U5W6Q8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561230.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33175"/>
    <dbReference type="EnsemblBacteria" id="AGZ44893">
        <property type="protein sequence ID" value="AGZ44893"/>
        <property type="gene ID" value="AFR_33175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6713"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_11"/>
    <dbReference type="OrthoDB" id="1186478at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24433" checksum="AD8D4E92F6804AF5" modified="2014-01-22" version="1">MRHACVIGNTDGIGLALTRRLLDEGWAVTGLSRRPAAVEHDRYVHVAADVTAPGYAAELTRALDAAGGADLCVYAAGVGEFLDLDNLAAQTRIIEVNLLGATRTADVVVPRMLAAGSGHFVGLSSLADAMITPMAPGYAASKAGLSTYLISLGAAVRPRGVQVTAVRFGYVDTKLALGPVKPMMITVDRAVDVLMRCIRTRPAVVSYPRRMAALIRAVSPLARTQARLKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W706</accession>
    <name>U5W706_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Anaerobic sulfatase-maturating enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_33625</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561320.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33625"/>
    <dbReference type="EnsemblBacteria" id="AGZ44983">
        <property type="protein sequence ID" value="AGZ44983"/>
        <property type="gene ID" value="AFR_33625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6805"/>
    <dbReference type="eggNOG" id="COG0641">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_10_2_11"/>
    <dbReference type="OrthoDB" id="1172757at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026335">
        <property type="entry name" value="SAM_SPASM_FxsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="PTHR43273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04269">
        <property type="entry name" value="SAM_SPASM_FxsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="27"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="405" mass="44335" checksum="139053CAFCEDD365" modified="2014-01-22" version="1">MTVHQVAVAPRWPDRELDVDAVRRSGRRPTPFRDVVLKVHQRCNLACDYCYVYTMADQSWRDQPLVMTRETWRATAGRMAGHAATHDLPTMRLVLHGGEPLLAGRDRLRELITDFRAAFTGGCRLDLQIQTNGTLLDEPILDLFAEHGVGVGVSLDGVAADNDSRRRYPDGRGSAGVVDRGLRLLTGPRYSHVFDGLLCTVDPSTDPLACYEALLTYRPPAIDLLLPHANWSDPPHRPATGSVTPYADWLITIFDRWYDAPSREVSIRLFEDVMALLLGGAGRSEQVGLSPVAVVVVETNGAIELVDSLKSAYPGACATGLTVFADTFDAALDHPGVVARQIGAAALSETCQACPLHRVCGGGHYAHRYRAQDGFRNPSVFCADLAALIRHVHQRMAGDLAQRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7L7</accession>
    <name>U5W7L7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43990">
        <property type="protein sequence ID" value="AGZ43990"/>
        <property type="gene ID" value="AFR_28645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28645"/>
    <dbReference type="eggNOG" id="ENOG5032VDB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340497_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="10726" checksum="29936CB237E02DDD" modified="2014-01-22" version="1">MEATASMEVDVAFFDDPGDRKADQVDGAIGELSPFHEMNGYYAQGVSVTTATLPDGWRDRLVVVESRSTHPGRGLTLDPHVHPAVLKRLRAWVGMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W817</accession>
    <name>U5W817_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560398.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44061">
        <property type="protein sequence ID" value="AGZ44061"/>
        <property type="gene ID" value="AFR_29000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5876"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_0_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="32357" checksum="6E1DD749A083ACFB" modified="2014-01-22" version="1">MRVTTPYGAKSTADEVSEGIDLTGKRAVVTGGASGIGVETARTLARRGASVTLAVRDVERGRKVADDIGPGVEVRPLELTDYASIAAFAEGWEGPLHLLVNNAGVMALPSLTPDPQGWEFQFATNHLGHFCLTTRLHSALAAAGGARVVSLSSSAHLFSPVVFDDINFKFRPYDATLAYAQSKTAVNLFAVAATERWRNDGITVNAANPGAIVTPLQRHVGGKLATPVELQKTTEQGASTSVLLAVSPLLAGIGGRYFNDNQEGVPTDQRPAEVEELVRSVAPYSLDPENAERLWVLSAAAVGRTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W890</accession>
    <name>U5W890_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_29645</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aminoglycoside phosphotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560527.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29645"/>
    <dbReference type="EnsemblBacteria" id="AGZ44190">
        <property type="protein sequence ID" value="AGZ44190"/>
        <property type="gene ID" value="AFR_29645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6009"/>
    <dbReference type="eggNOG" id="COG3231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073027_0_1_11"/>
    <dbReference type="OrthoDB" id="1457558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05150">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024165">
        <property type="entry name" value="Kan/Strep_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000706">
        <property type="entry name" value="Kanamycin_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="5">
        <location>
            <begin position="13"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000706-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000706-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="25195" checksum="1DE29A58B2BEC30C" modified="2014-01-22" version="1">MWEIVTTGQSGATVSRRDGVYRKTSNDPRDDLPGEAARLTWLRAHGIPAAQVLSSAPGLLETAEVPGLPASDYRHGVVDALADFTRVLHALPIADCPFDRRLAVTIPAALAADVDLDDLDDERAGWTRDELVAQLHATRPVQEDLVVCHGDLCLPNVVLDREGSQVTGLIDVGRLGVADRWTDLALMTRSLSDPGLNPGYGPWAADRFLTRYGIAPDPAKNDFYRLLDEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8B2</accession>
    <name>U5W8B2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560552.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29770"/>
    <dbReference type="EnsemblBacteria" id="AGZ44215">
        <property type="protein sequence ID" value="AGZ44215"/>
        <property type="gene ID" value="AFR_29770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6032"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_4_4_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="16564" checksum="D1334CD1899FB330" modified="2014-01-22" version="1">MTQPETLGPVESAVGYVLKQAAVALRGAMDAVLRPMQLTVPQYSCMELLAQRPGLSNAELARGTFVTRQSMNVVLQGLEERGLVTRPAKAVKGRELPAALTEAGRSLLYAASAAIRSVNEQMCAGLTSAQEKALLETLTMLIRNLDPASTGPDLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8E7</accession>
    <name>U5W8E7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative PadR-like family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45483">
        <property type="protein sequence ID" value="AGZ45483"/>
        <property type="gene ID" value="AFR_36135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7313"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="2">
        <location>
            <begin position="57"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="20721" checksum="B3BE7DF63272AD3E" modified="2014-01-22" version="1">MPHMHEGRRRGFGFPPGFPFGQGTPGGFGGGFGPGGPGGHGHRRGGRGSRPNVRPALLALLLERPMHGYEMIQELESRTGGIWRPSPGSVYPTLQLLEDEGLIVAEATGGRKRFTLTDAGREEAAAASENPPWAQFSNDTMSQVQDFRDAAVGIMDALRQVGFNGTPEQRQKALEVLNETKRKLYAILADGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8G0</accession>
    <name>U5W8G0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30025"/>
    <dbReference type="EnsemblBacteria" id="AGZ44265">
        <property type="protein sequence ID" value="AGZ44265"/>
        <property type="gene ID" value="AFR_30025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6081"/>
    <dbReference type="eggNOG" id="ENOG5032YGT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094531_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="188" mass="20531" checksum="0EEB8F5BC4CA7BC0" modified="2014-01-22" version="1">MVGFGGKQAWLAVRDGDPAQVLEALGLRDLGEVRWRDGIDLAHLSDDRVAVTPPLPGARDTSWVLAAGRYLMRPDTKLDVIELSQRLHTEVQFFSTHRVTESHRWQRAVDGELVRAFGYVGQTGDVTSWHGEPDPAERSAGLPATFVDEETSILVSEQDVLKVADGWSINPARLDGKAPGPLRVGAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8W1</accession>
    <name>U5W8W1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative sugar ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560770.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30875"/>
    <dbReference type="EnsemblBacteria" id="AGZ44435">
        <property type="protein sequence ID" value="AGZ44435"/>
        <property type="gene ID" value="AFR_30875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6250"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_10_1_11"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665814" evidence="1">
        <location>
            <begin position="29"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="420" mass="43413" checksum="6AB40289177B7E95" modified="2014-01-22" version="1">MRLRLAATAALALTITACAPSTSSPSSAPSDGGERTGTLKVWLFDEPNRPPKEGVVQAAIAEFTAAHPGTKVDVAYIPVETRSERFKGAFNDPASAPDVVEYGNTDLPEYAAAGGLADLGATVSGWAEGADLGADAKASATYQGTLLGVPWFVGVRALYYRTDVFAELGLKAPASQADLVTAAHTIRAKKPEMFGIAVGGKYTFGALPFVWDAGGDITSINSPQSRTGLKVYTDLIRADNCPPQQCADLTGGKTVDLFASGKAGMGIIGNFNRAAVDAGAAKGKYAVVPLPGSQPGSIAPAFAGGNNLGIMKNSRRPALAADFLTLLAGKKYQARMYAAMGNLPTLKSARDEVAGKDPFLKPFLSTIDAGTKFVPLDPGWGKIDASAVLPTMIEKVATGRATVEQATDEAATAISAALTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9B4</accession>
    <name>U5W9B4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562133.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37720"/>
    <dbReference type="EnsemblBacteria" id="AGZ45798">
        <property type="protein sequence ID" value="AGZ45798"/>
        <property type="gene ID" value="AFR_37720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7628"/>
    <dbReference type="eggNOG" id="COG2335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031281_3_1_11"/>
    <dbReference type="OrthoDB" id="1776069at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="SSF82153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665825" description="FAS1 domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="2">
        <location>
            <begin position="78"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="210" mass="20868" checksum="142F5F3C3277B45C" modified="2014-01-22" version="1">MRATKFTALTAALLFSASLAACGGDDAAEDTGSAAPATTTASAAPSMPASDSMANFGAGCAAVPTDASNPGSFDAMSQVPVATAASGNPLLSTLVTAVKKAGLVDTLNTAPALTVFAPTNDAFGKIPKADLDKVLADNKTLTSILTYHVVEGKLTPAELAGTHKTIEGGSVTIKGSGSDFKVNDASVVCGNVQTANATVYIIDSVLMPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Q4</accession>
    <name>U5W9Q4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="3">DAP decarboxylase</shortName>
            <shortName evidence="3">DAPDC</shortName>
            <ecNumber evidence="3 4">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lysA</name>
        <name evidence="8" type="ORF">AFR_37490</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="3 4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562087.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37490"/>
    <dbReference type="EnsemblBacteria" id="AGZ45752">
        <property type="protein sequence ID" value="AGZ45752"/>
        <property type="gene ID" value="AFR_37490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7583"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_1_11"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3 4" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="7">
        <location>
            <begin position="72"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="6">
        <location>
            <begin position="330"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="3">
        <location>
            <begin position="318"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="461" mass="49103" checksum="5CFCC8B4518625D4" modified="2014-01-22" version="1">MRAHEAGALHGDHGNRGPAWLRTPEDVNGLVPQLWPRTVTRADAGALEIGGVSVLDLAAEYGTPAYFLDEEDLRSRCRDFASAFAGADVYYAGKSFLCKAVVKVVNEEGLFLDVCTGGELAVALAAGFPAERMGFHGNNKSLSELTRALDAGVGRIILDSFQEIDRLTALATERGVRPKVLIRVTVGVEAHTHEFIATAHEDQKFGFSLAGGAAFEAAIRVLDEGVLDLHGLHSHIGSQIFDTSGFEVATRRVVELQAQIRDARGVELAELDLGGGFGIAYTTQDDPSPAADLAKRINKIIASECDASSLKIPKLSIEPGRAIVGPSVFTLYEVGTVKDVDGIRTYVSVDGGMSDNIRTALYDASYSATLAGRKSEAPPLLARVVGKHCESGDIVVKDEFLPADVQPGDLLAVPGTGAYCRSMASNYNHVPRPPVVAVRDGASRVIVRRETEDDLLALDVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Y1</accession>
    <name>U5W9Y1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562162.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37865"/>
    <dbReference type="EnsemblBacteria" id="AGZ45827">
        <property type="protein sequence ID" value="AGZ45827"/>
        <property type="gene ID" value="AFR_37865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7657"/>
    <dbReference type="eggNOG" id="ENOG5031QXP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079037_0_0_11"/>
    <dbReference type="OrthoDB" id="2059682at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="23308" checksum="F093A943AF8CF79A" modified="2014-01-22" version="1">MRLPTFSRQSDTTETEERPAVATSSTTPVRQRTDPPTATMPTVTPPPAAPPTVVERRRSADEDTTADLKSPDLRAPERRPADRNADEKTIEQPPVTGSPADRTAARPAEPPVVAGPKPRASLLATLGLIVSVAAALLVLSGPLLGYGIGLGVLGLILSLGGISATGKRHIAGKTDALIGMILSLGAIVVGVLALTGSLSWLGTDMQPVTSVREWLDAQFVNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA91</accession>
    <name>U5WA91_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39220"/>
    <dbReference type="EnsemblBacteria" id="AGZ46098">
        <property type="protein sequence ID" value="AGZ46098"/>
        <property type="gene ID" value="AFR_39220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7936"/>
    <dbReference type="eggNOG" id="ENOG5033C4E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095244_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025339">
        <property type="entry name" value="DUF4245"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14030">
        <property type="entry name" value="DUF4245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="19833" checksum="89C3BC9C040D76AD" modified="2014-01-22" version="1">MTTPDAAAEPVAVGKRGGRSPRDMAMSLGVLLVPIALLLLFYRLVLDGDKPVSVDVEPTLQQARSAAVFPVLAPQGLSDDWHVMSATFKRDTAGATLRIGYVDPDRDPVQLVESSLPTATLLPVELGKDPKATGTFRDGPRTWQRYDARQGEYALLLIEKGRTVIVVGTTETETLEKFASSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAB8</accession>
    <name>U5WAB8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38590"/>
    <dbReference type="EnsemblBacteria" id="AGZ45972">
        <property type="protein sequence ID" value="AGZ45972"/>
        <property type="gene ID" value="AFR_38590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7808"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="96" mass="11035" checksum="9114F7C32BB73B01" modified="2014-01-22" version="1">MLANPVRRQLLVALRDGPRAAGDLAGSFALSRPAVSEHLAVLRHARLVREEPRGRHRYYHLAAEPLADVQEWLNPFQRYWRERLTALKDLVEGDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAF8</accession>
    <name>U5WAF8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38765"/>
    <dbReference type="EnsemblBacteria" id="AGZ46007">
        <property type="protein sequence ID" value="AGZ46007"/>
        <property type="gene ID" value="AFR_38765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7844"/>
    <dbReference type="eggNOG" id="ENOG502ZET7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1694275_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="149" mass="15941" checksum="41C0015938FF8F78" modified="2014-01-22" version="1">MPIAVLAAALFAINVVARVVIRIGFADNDSAQNTVSIIMFAVIGVVLAVVAFIRAQRVPPSSWLPELAASAIGGMLLTILIGPFISGDTPFAAGAGNFFSQIWLYGGFAIVGTLLGYWVATMLGRDYRSRSLQAFARAKTTKPRRVVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB86</accession>
    <name>U5WB86_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_40775</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40775"/>
    <dbReference type="EnsemblBacteria" id="AGZ46409">
        <property type="protein sequence ID" value="AGZ46409"/>
        <property type="gene ID" value="AFR_40775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8254"/>
    <dbReference type="eggNOG" id="COG0581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="127"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="348" mass="36587" checksum="47C672CE30A1501B" modified="2014-01-22" version="1">MTPDNIRTKKLPIAVNLGVAVAALVVSAALVLGLGLGNWVLVVFVGAVLYLAALFAAATRVEGRRSARNRIWKTLIYSACILAILPLASVVWTLVSKGAARLDGDFFGSSMNNIGARDANGGAYHAIIGTLEQVGIATLMAVPLGVLGAIYLVEYGRGKFALTVRFFVDVMTGIPSIVAGLFILAFWVLIVSPWFNNGTPRFSGFAASLALTVLMLPTIVRSTEEMLRLVPGPLREGAYALGVPQWKTILKVVLPTALPGIVTGVMLAVARAAGETAPVLLVAGGAAAINFDAFAGNQSSLALYVYQQAGDASRYAPDRAWTAALTLVALVLVLTITAKLLARRNRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBD3</accession>
    <name>U5WBD3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_34975</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34975"/>
    <dbReference type="EnsemblBacteria" id="AGZ45251">
        <property type="protein sequence ID" value="AGZ45251"/>
        <property type="gene ID" value="AFR_34975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7077"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="266"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ45251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="421" mass="45307" checksum="C0E46AEE34D7AB08" modified="2014-01-22" version="1">MLVLVLVLVNAAFSGSEMALVSLRDSQVNKLERQSRRGRVLGRLTRDPNRFLATIQIGITLAGFLASAAAAVSLSKPLVEPLGFLGSAAEPAAIVVVTIVLTFVTLVIGELAPKRIAMQRTEGWALLVARPLDILSTISRPAVWLLSVATNLLVRLAGGDPKAQREEVSTEEIRDMVAAQQDFSVEQRTIISGAFEIADRILREILVPRRDVLTLATGLAAPEALTQLIAGGHSRAPVVGPAGLDDVFGVVHLRDLVGAQGPVDLVARPGLFLPETLRVSDALRQMRLERHQLALVVDERGAIDGIITMEDLVEEIVGEIYDETDRDVQSVVKEADGALLMPGSFPIHDLPDIGFDNSVPDEGDYTTVAGMVLASLGHIPTEPGEVVTLPQFTAEVVEVTGRAITRLRLRALPREEPEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBD9</accession>
    <name>U5WBD9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561631.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35195"/>
    <dbReference type="EnsemblBacteria" id="AGZ45295">
        <property type="protein sequence ID" value="AGZ45295"/>
        <property type="gene ID" value="AFR_35195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7121"/>
    <dbReference type="eggNOG" id="COG4034">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800915_0_0_11"/>
    <dbReference type="OrthoDB" id="1337686at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010581">
        <property type="entry name" value="DUF1152"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06626">
        <property type="entry name" value="DUF1152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="315" mass="33857" checksum="2F5B402186107BB1" modified="2014-01-22" version="1">MIKPPLFTALEGARSVLLAGAGGGFDIYAGLPLAFALRDAGVDVHLANLSFTQLGLLDLEAWLDPDLALIMPDTRGPDDYFPERTLARWLAAHDMPPAVHAFPRTGVQPLRAAYQNLITRYAVDAIVLVDGGTDILMRGDEAGLGTPEEDMASLGAVAGLDVPTRLVTCLGFGIDAYHGVNHVQVLENLATLDRDGAYLGALSIPGHGREAALYRDAVADAQATTPMRPSIVQGQIAAATRGASGNEQFTTRTADSTLFVNPLMAVYFTVDLPALARHNLYLDRLEDTVGMRQISTRIERFRAGITPRLARTYPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBK0</accession>
    <name>U5WBK0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561667.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35375"/>
    <dbReference type="EnsemblBacteria" id="AGZ45331">
        <property type="protein sequence ID" value="AGZ45331"/>
        <property type="gene ID" value="AFR_35375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7158"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_2_2_11"/>
    <dbReference type="OrthoDB" id="1775418at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="26094" checksum="EBB84319921403B6" modified="2014-01-22" version="1">MANTPLYVRIERALRARLVTARPGDGVPAESALAEEFGVARMTVRAALNALEADGLIERIQGRGTFVRQRPAPRAAGTLMSFHDQVRSWGRRPSSRLVEGEVRPATAEEAAALQLTAVSPQVVAITRVRLADDVPLAVEYACFPPHLDGLLEADLETGSLHRALHERGVRPTLGSSTLTAQPAGRDGGRLQVPDGEPLLVETRLIVDQHAAPLEYTVSRYVGSRYSLQVAFDVQPPREGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBW8</accession>
    <name>U5WBW8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative peptide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_41775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562940.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41775"/>
    <dbReference type="EnsemblBacteria" id="AGZ46609">
        <property type="protein sequence ID" value="AGZ46609"/>
        <property type="gene ID" value="AFR_41775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8454"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_11"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="49"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="345" mass="38185" checksum="25AA7C4A8BDF1A66" modified="2014-01-22" version="1">MSENLLEVSGLQKHFPITRGLFKRQVGAVRAVDGIDLSVRNGETLGLVGESGCGKSTAGRLFTRILEPTGGKIVFEGQDISHLSAEKMRPLRRDVQMIFQDPYSSLNPRHTVGSIIAAPLQIQNIPTPNGVKKAVQELLELVGLSPEHYNRYPHEFSGGQRQRIGIARALALRPKLIIADEPVSALDVSIQAQVVNLLDDLQREFDLTYVFIAHDLSVVRHISDRVAVMYLGKVVEIADRNQLYKNPRHPYTVALMSAVPVPDPSRRDRAQRERVLLTGDVPSPINPPSGCRFRTRCWKAQDICAQEEPPLVARLDDPESHLTACHFPVAEEEVVVGRAPAKTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBY0</accession>
    <name>U5WBY0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2263 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42245"/>
    <dbReference type="EnsemblBacteria" id="AGZ46703">
        <property type="protein sequence ID" value="AGZ46703"/>
        <property type="gene ID" value="AFR_42245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8552"/>
    <dbReference type="eggNOG" id="COG4295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024412_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012664">
        <property type="entry name" value="CHP02452"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019261">
        <property type="entry name" value="PARG_cat_microbial"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10021">
        <property type="entry name" value="DUF2263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014899">
        <property type="entry name" value="UCP014899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02452">
        <property type="entry name" value="TIGR02452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2263" evidence="1">
        <location>
            <begin position="5"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="26775" checksum="93118C83CE63F761" modified="2014-01-22" version="1">MRAIAKDTVEIAERGSYIDPGGHQVRIDAGPAIAGTRLYLPEDPLPEGRSAGSNRIEVTGESTLIAARRLGGDVAALVFASARNPGGGFLNGAQAQEESLARASALYPCLRAAGDFYAHHRAQTELTYTDRVIYSPAVPVFRDDKGTLLTEAYPVTFLTSAAPNLGAIRRSQPHLAADVPAILHRRAVRVLAVAAAHGHRRIVLGAWGCGVFANDPATVAEAFRLALEQQPMDEVVFAVLGPNRDAFTRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC13</accession>
    <name>U5WC13_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AFR_42420</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563069.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42420"/>
    <dbReference type="EnsemblBacteria" id="AGZ46738">
        <property type="protein sequence ID" value="AGZ46738"/>
        <property type="gene ID" value="AFR_42420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8585"/>
    <dbReference type="eggNOG" id="COG0266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038423_1_2_11"/>
    <dbReference type="OrthoDB" id="1162346at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="DNA_glyclase/AP_lyase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="MutM-like_N-ter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000214">
        <property type="entry name" value="Znf_DNA_glyclase/AP_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="SSF81624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51066">
        <property type="entry name" value="ZF_FPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FPG_CAT" evidence="16">
        <location>
            <begin position="2"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="FPG-type" evidence="15">
        <location>
            <begin position="237"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ46738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="30962" checksum="AF7F05FAC57BD5BD" modified="2014-01-22" version="1">MPELPEVEALAAYLRERAVGHAVERFEVSSFSALKTYDPPPSAVAGLVITSAGRHGKFLDLTFGDDLHLVVHLARAGWLHYRDAFKSPLPLKPGSGPIAIRVRLDDGSGFDLTEAGTQKSLAAYLVRDPAEVPGVSRLGPDALAVSRDEFAERLRSRRGQVKGVLTDQEVLAGIGNAYSDEILHVAKMSPFALTSKLTDEQITTLFDAMRQVETDAVTRSVGQKAAELKGEKRAGMRVHARTGLPCPVCGDNVREVSFADKSLQYCATCQTGGKPLADRRLSKLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCC2</accession>
    <name>U5WCC2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562948.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46617">
        <property type="protein sequence ID" value="AGZ46617"/>
        <property type="gene ID" value="AFR_41815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8462"/>
    <dbReference type="eggNOG" id="ENOG502ZHXG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074084_0_0_11"/>
    <dbReference type="OrthoDB" id="1802206at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="30004" checksum="E3895CCDF4FBAE59" modified="2014-01-22" version="1">MENSGAQWPDPAQPIGPPATPPPWQAPPVPPPAPKSHTKLIAVIATVGLLAVACAGSVAFGAIKALRDTPATPTAPAAPRALSEAGPSASSVPTSAAPARPVGVTGKVPAGSKASSLKVRKSEDLERVCDRWYYPKSPKYTTAVAPHPIVVSVRDRKDLDFRTTKSYLGIPYDAPEAIKAAWEGKNPAKVQLVACVDLVTIGSKVKSCKIDKPKKSSIPMKEGTYRLSLYEAATRRKIFETKLVGEDETCPIFIIIGNDRSVYSGLEDRQLVDALQRYVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCE4</accession>
    <name>U5WCE4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative 1L-myo-inositol-1-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563159.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42875"/>
    <dbReference type="EnsemblBacteria" id="AGZ46829">
        <property type="protein sequence ID" value="AGZ46829"/>
        <property type="gene ID" value="AFR_42875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8674"/>
    <dbReference type="eggNOG" id="COG1260">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050011_0_0_11"/>
    <dbReference type="OrthoDB" id="669352at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017815">
        <property type="entry name" value="Myo-inos-1-P_Synthase_actino"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03450">
        <property type="entry name" value="mycothiol_INO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inos-1-P_synth" evidence="2">
        <location>
            <begin position="191"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="359" mass="39014" checksum="A486B998A47AEBD5" modified="2014-01-22" version="1">MGSVRVAIVGVGNCASSLVQGVEYYREADPNDRVPGLMHVTFGDYHVSDVKFVAAFDVDAKKVGMDLAEAIVASENNTITLTDVAPTGVTVQRGPTFDGLGTYYREMVEESSAEPVDIAQALRDAEVDVVVSYLPVGSEEADKFYAQAAIDAGCAFVNALPVFIASDPAWAQKFTDAGLPIVGDDIKSQVGATIVHRALAKLFEDRGVELLRTYQLNFGGNMDFMNMLERKRLVSKKISKTQSVTSQIPHEMVKSDVHIGPSDHVPWLDDRKWAYIRLEGRSFGDTPLNAELKLEVWDSPNSAGVIIDAVRAAKIALDRGVGGPILSASSYFMKSPPVQYNDHDAKNAVEAFIKGDIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCW2</accession>
    <name>U5WCW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sodium/hydrogen exchanger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_37585</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562106.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37585"/>
    <dbReference type="EnsemblBacteria" id="AGZ45771">
        <property type="protein sequence ID" value="AGZ45771"/>
        <property type="gene ID" value="AFR_37585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7602"/>
    <dbReference type="eggNOG" id="COG0475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_7_0_11"/>
    <dbReference type="OrthoDB" id="941006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="13"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="417" mass="43010" checksum="594EB165490491CF" modified="2014-01-22" version="1">MTPTELAPRFFIAVAVILLFCKGVAWLLGKAGQPAVVSEMLAGVLLGPSLLGLLLPDVKDALFPAQLLPILYVVGQVGLVLFMFQAGYAFTAHRVSGLAGTAGMVSLAGVTAPLVLGVLLVLVTSGAVPIQAEGTALGVSAAFVGVALAITAFPMLARIITERGQAGTRHGTLSLASGAIDDLVAWIMLAVVLAFASGEAGPAVVTAGGAVLFALVLWYGGRRTTTALMTSGRLDDRARLLGTVVLLFLVAWYTDEIGLYAVFGAFAVGVVMPRTAATDRVVDTLTPIAATVFLPLFFTYSGLRTEFGLLGNPAVLLFAVACVTVAIVGKFGACWAAARLRGEPQGVALRVGALMNARGLMQLIALNVGLEAGIVNSSLFTVLVLVALITTLMTTPLLSWIDRSSKPAPAPVLVDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDA4</accession>
    <name>U5WDA4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ectoine hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562266.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38385"/>
    <dbReference type="EnsemblBacteria" id="AGZ45931">
        <property type="protein sequence ID" value="AGZ45931"/>
        <property type="gene ID" value="AFR_38385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7765"/>
    <dbReference type="eggNOG" id="COG5285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047725_3_1_11"/>
    <dbReference type="OrthoDB" id="1070946at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="32983" checksum="8CAD68D4F97D19CA" modified="2014-01-22" version="1">MSLLAEQTWTPMTAEERAQFDRDGFLVIPAVLTEDEVAIARTAILRSKEQVSREGGLGATGALHQLSAVTNNPELAFLLDHPKAFKYIWSILGWNVHVYHSHVDVHPQIHEKQKDWWHWHQDGGRQNRELETDPRPRTSVKLAYWLSDVSETGRGNFTVLPGSHLTNWLPGPPSRGVPWPQPEGALQITANPGDLVVFDRRLWHARSDNYSPITRVGAFFGYTYRWVAGRDEVKDLPNTPEWAGYNPVQQQLLGHCGDGSGDHAWGHFPETTPLYGELKRQGLLDSSVPALIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDI4</accession>
    <name>U5WDI4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Biotin carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562379.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38955"/>
    <dbReference type="EnsemblBacteria" id="AGZ46045">
        <property type="protein sequence ID" value="AGZ46045"/>
        <property type="gene ID" value="AFR_38955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7884"/>
    <dbReference type="eggNOG" id="COG0439">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029016_5_0_11"/>
    <dbReference type="OrthoDB" id="1677960at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040570">
        <property type="entry name" value="LAL_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18603">
        <property type="entry name" value="LAL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="83"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="404" mass="43598" checksum="925CB834838B304A" modified="2014-01-22" version="1">MTQQSIIILGAGEDQLPAYLTARRLGYRVIGVDRNPGALAAYAADEFLCLTSRDPAAIAERLGAIDVAAVLSPASDAAQASVAALSAHYGTAFRPAPLAVRASEDKAFFHEVVTKLGFPTYRHGRGPSGLRLPVVVKPTDSSGSKGLTCVRSAGDLDAALALARRHSFTGEVIVEERVEGRHYSAECFAQNGSPVFVAVTERVMTPEPEMITVSHVLPAELGEDVRDRLDEMLAALFAELGHRSGPINVDFVLDAEQRIHFIEMGARLGGNGMPMLVRHAFGLDTVEAAIRLALGESLDLSTGRQQRYAMLHILTAATDGTLTAIDGVEETRELPVLAELRLFKNVGDPVRRYTQAAHKLGYLVLTADHREDIRRGLDTALATLHFRIAAKAPRQHSENLEMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WET9</accession>
    <name>U5WET9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH-quinone oxidoreductase subunit D</fullName>
            <ecNumber evidence="5">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NADH dehydrogenase I subunit D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NDH-1 subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nuoD</name>
        <name evidence="8" type="ORF">AFR_41405</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562867.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41405"/>
    <dbReference type="EnsemblBacteria" id="AGZ46535">
        <property type="protein sequence ID" value="AGZ46535"/>
        <property type="gene ID" value="AFR_41405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8379"/>
    <dbReference type="eggNOG" id="COG0649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_11"/>
    <dbReference type="OrthoDB" id="473681at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="PTHR11993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 5 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex1_49kDa" evidence="7">
        <location>
            <begin position="128"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Complex1_49kDa" evidence="7">
        <location>
            <begin position="300"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="374" mass="41389" checksum="637521BBF28BAD3E" modified="2014-01-22" version="1">MTVGTGSGLDTADMVLNIGPQHPSTHGVLRLKLTLDGERVVTCEPIVGYMHRGAEKLFEVRDYRQIIVLANRHDWLSAFSNELGVALAVERLMGIEVPERATWLRMALAELNRVLNHLMFLGSYPLEIGAITPMFYAFRERETLQAVMEEVSGGRIHYMFNRVGGLKEEVPAGWTSRARTAIDKVRSRMPDIDNLVRNNDIFRARTVGVGVLTAEQAAAYGASGPVARASGLDIDLRRDEPYLAYDQLDVPVVTRTAGDCHARFEVLLDQVYASLDLAQACLDRVDRISGPVNVRLPKVVKAPEGHTYAWTENPLGINGYYLVSRGEKTPWRLKLRTASYANVQALATLIPGCLVPDLIAILGSMFFVVGDIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VN93</accession>
    <name>U5VN93_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357387.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00770"/>
    <dbReference type="EnsemblBacteria" id="AGZ38443">
        <property type="protein sequence ID" value="AGZ38443"/>
        <property type="gene ID" value="AFR_00770"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00770"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.155"/>
    <dbReference type="eggNOG" id="ENOG5032IA4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929534_0_0_11"/>
    <dbReference type="OrthoDB" id="922446at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="31208" checksum="04BB701A6CB49541" modified="2014-01-22" version="1">MPSLFASYLRVYEPLTAFDRDRQIFWRRYAREGRALGPVEGPVRQRTAVLEALGAGWTRLPDLPDEAYVMEWADTLLVCPWNLRLRVAEAALNARDGVPSVLADAFVPPVLAGQAKAIVEDWRSGAKVLEHGVPRVHEQIATWGVPLRWFVFVDLDEREINLADGKRTLRYRTEISKARRRAHRAVSVLRKSLGDAPITEAVEEGTRWLEEFHPRSIVELDYGGLTGLLDAEALSEDDSPGLVAAGLSALSRGDADAATEAYEKLVARWRAVQLLERCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPI6</accession>
    <name>U5VPI6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02190"/>
    <dbReference type="EnsemblBacteria" id="AGZ38727">
        <property type="protein sequence ID" value="AGZ38727"/>
        <property type="gene ID" value="AFR_02190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.443"/>
    <dbReference type="eggNOG" id="COG0079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="19"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="393" mass="44613" checksum="0F3FB92D746809DB" modified="2014-01-22" version="1">MDEGAEIVDFCIPCNPYFPTPEMFDELSRDLKNILKYYPSDSSSITNKLASVLGLHPQTVAMANGSTELITWIDHLLIKESIAIPIPTFGRWTDQPLETGKRVDMFPLQERDGFRLNLDDYVRFIRDRGSRVAVVCNVNNPDGGYLARRDVIRFMDALGDLDLVVIDESFIDFSDAEQYPSVGPDAVIRPNTVVLKSLGKNFGLHGIRFGYLMANPAIAGRIGKALPKWNLNSLAEKVVYMIQEHDDEYEDSLRLLSRDRRTMAGELARIPGLTVFPSQGNFILVKLPTEWSGVSLRDYLVANHGVYTRECGNKLGMTSQFMRLVVRPARDVDRLIDGMLDYGRQFRTRPAWEDEPVETGRRWTTTVDEEPDNLIQYPTHRTTEYTARRAVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPK8</accession>
    <name>U5VPK8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_02290</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357690.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02290"/>
    <dbReference type="EnsemblBacteria" id="AGZ38747">
        <property type="protein sequence ID" value="AGZ38747"/>
        <property type="gene ID" value="AFR_02290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.463"/>
    <dbReference type="eggNOG" id="COG1682">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060703_4_0_11"/>
    <dbReference type="OrthoDB" id="1543624at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="9">
        <location>
            <begin position="56"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ38747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="32764" checksum="384AA912E925EB81" modified="2014-01-22" version="1">MPPTAVADSETGLSLKELARKHGLSAAGKLPSLRDYSRQLWSYRHFITSFANAKVSSALGRTRLGIFWQVLTPLINAAVYYVIFGVVLNTKSGIDNFVAYLCTGVFIFGFTQSVVQAGIQAISGNLGMIRALHFPRASLPLAIVMVEIRNMIASLAVLMVIVLAFGERITWEWLLIVPIFLLQSIFNAGLAMAVARLGSKVADFKQLVPFIMRIWLYGSAVLYPVTRFTEHLSGWKLAVVEANPLLVFIDLMRHALMEDVPLAAPAGVLWLEAVAWALVAGLGGYVYFWRGEKGYGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPM8</accession>
    <name>U5VPM8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative cysteine dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357710.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02390"/>
    <dbReference type="EnsemblBacteria" id="AGZ38767">
        <property type="protein sequence ID" value="AGZ38767"/>
        <property type="gene ID" value="AFR_02390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.483"/>
    <dbReference type="eggNOG" id="COG5553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102185_0_1_11"/>
    <dbReference type="OrthoDB" id="1571420at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="PTHR12918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="17319" checksum="28F59589C2ADF7FA" modified="2014-01-22" version="1">MTVVDIRLDHLATATRYAADPDSWPVAPRYNAADRWYHRLAQEPDHEVWLLTWLPGQGTDLHDHGGSAGAFYIHSGTLTEDTVATSPDGLPRITARELGEGAGRRFGTRHIHRITNRSNRPAISIHAYGPALSTMTRYRIGAAGLDVVTVERAGAQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPN9</accession>
    <name>U5VPN9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribose transport system permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357720.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02440"/>
    <dbReference type="EnsemblBacteria" id="AGZ38777">
        <property type="protein sequence ID" value="AGZ38777"/>
        <property type="gene ID" value="AFR_02440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.493"/>
    <dbReference type="eggNOG" id="COG1172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028880_3_0_11"/>
    <dbReference type="OrthoDB" id="1254678at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="33678" checksum="E0383048BD393267" modified="2014-01-22" version="1">MSEATATVSRPAIDLARIKAWLPAYGVYVAIALLLVYNVLFTPNFLAWSNLRIQLIQVAPIVIVALGMALVIGTEGIDLSVGSVMALAAALIPLYLGYGVIAAILVSLVAGVLVGLINGTLVARVGLQPIVATLALFVGGRGLALVISDGQLKDVRNDDLIYLGSGDFLRIPVLVWIAALLVLVVAFVVRRTVFGRRLLAIGGNRPATELAGVPVKRVLITVYVVCSVLASIAGLLAVARIQSSDASAVGLLIELSAITAVVVGGTPLTGGKVRVLGTVAGALLMQLVVATMIKHNLQPSTTEMVQAVIILIAVYVARERKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPZ4</accession>
    <name>U5VPZ4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDT1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02925</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GDT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357817.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02925"/>
    <dbReference type="EnsemblBacteria" id="AGZ38874">
        <property type="protein sequence ID" value="AGZ38874"/>
        <property type="gene ID" value="AFR_02925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.588"/>
    <dbReference type="eggNOG" id="COG2119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040186_2_0_11"/>
    <dbReference type="OrthoDB" id="2050363at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="Gdt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="PTHR12608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="UPF0016"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="20464" checksum="94D95582540E5A79" modified="2014-01-22" version="1">MDGFLAATALSFAVIFVAELGDKSQLMALTFATRFKTVPVLIGITVATAIVHAVSVGIGYGLGATLPTGWISLVAGIAFLAFGAWTLRGDKLTDDEKSKAERSTGSAILAVGGAFFLAELGDKTMLATITLATQHGWLGTWLGSTLGMVAADALAILVGRLLGRHLPERTIKYGAAALFAIFGIWLLVEALIELNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ13</accession>
    <name>U5VQ13_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dyp-type peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_03065</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DyP-type peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03065"/>
    <dbReference type="EnsemblBacteria" id="AGZ38902">
        <property type="protein sequence ID" value="AGZ38902"/>
        <property type="gene ID" value="AFR_03065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.617"/>
    <dbReference type="eggNOG" id="COG2837">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044178_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006314">
        <property type="entry name" value="Dyp_peroxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04261">
        <property type="entry name" value="Dyp_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01413">
        <property type="entry name" value="Dyp_perox_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51404">
        <property type="entry name" value="DYP_PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="313" mass="34112" checksum="6968B65BAB31226B" modified="2014-01-22" version="1">MGPTVNQQAVLSPLTDAAIFLVATVREGGEQAVRDLLEDLGALQRSVGFRVPAAQLSCVTGIGSDLWDRLFDGPRPAQLHRFRQWKGPKHTAPSTPGDLLFHLRASQVDVCFELATKIGERLRGAADIIDETHGFKYFDERDLLGFVDGTENPVGAAASAAVLVGDEDPDFAGGSYVMVQKYVHDMTAWNTMTVEQQEAVIGRTKLDNIEIADKPADSHVALNTVEDHEGNELKILRDNMPFGSIAAGEFGTYYIAYAATPDVPELMLRRMFLGDPYGSYDRILDFSTARTGSLFFVPTGDFLDDLPPATSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQB8</accession>
    <name>U5VQB8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleoporin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357947.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03575"/>
    <dbReference type="EnsemblBacteria" id="AGZ39004">
        <property type="protein sequence ID" value="AGZ39004"/>
        <property type="gene ID" value="AFR_03575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.719"/>
    <dbReference type="HOGENOM" id="CLU_064711_1_0_11"/>
    <dbReference type="OrthoDB" id="2184073at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="254" mass="27631" checksum="C15C84272B6952E3" modified="2014-01-22" version="1">MDFAVALLVGQGGRAAAAIWATLILLALLALAGLALPRGVFRPRQISAWLADSAAQKRAELERRSTEAEELIRYADEIAVAARGAAHTAERRREECQEAQVRTQEAWQAYQAADAALTRARRAAAYATPMTDDEHADRAQALRRSAQSAYRRGDLSDTQLLDALTHRNGWDPTLHPVEQELILARAAVTHRFAAYQECLTAEDAAWQAADIATASVRSLRLEVIAAEAQAESARATLPQQTTPARSSRRIAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQC6</accession>
    <name>U5VQC6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357962.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03640"/>
    <dbReference type="EnsemblBacteria" id="AGZ39017">
        <property type="protein sequence ID" value="AGZ39017"/>
        <property type="gene ID" value="AFR_03640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.733"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071824_0_0_11"/>
    <dbReference type="OrthoDB" id="1311927at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="32743" checksum="5F68A6755E15F7F3" modified="2014-01-22" version="1">MNLSILYRGPLASCNYDCPYCPFAKRVDPPELLRADRAALERFAAWVEATTDVQLSVLFTPWGEGLTRSWYRDTIVRLSHLPHVERVAIQTNLAARVDWLAEADRNTAALWTTYHPGQITRERFLERCTGLRRLGVRFSVGVVGLPEHLAEARALRADLPDEVYLWVNAAEGHRYDAEQEAHWTALDPLFGHSVRPHLSLDRPCHAGETAISVQGDGSVRRCHFIAEPIGNLYDGSWRPALRPRGCTNAVCDCHIGYVHLKPLGLRDVFAGGVLERIPAAWPPQRATVSGAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQI7</accession>
    <name>U5VQI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_04620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04620"/>
    <dbReference type="EnsemblBacteria" id="AGZ39213">
        <property type="protein sequence ID" value="AGZ39213"/>
        <property type="gene ID" value="AFR_04620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.933"/>
    <dbReference type="eggNOG" id="COG1008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102484_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012551">
        <property type="entry name" value="DUF1707"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08044">
        <property type="entry name" value="DUF1707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="16084" checksum="1F0B581B96E1238A" modified="2014-01-22" version="1">MERNDMRAGDSERQATADKLKTALDEGRLDLHEYDERLQKTYSAKTFGDLDTLVTDLPGTIPTQQSRVEPYQAPSAPQTAATTGKKSKQQGAGQFVVSYGGVVLVCVIIWALTSLGSGELRYFWPGWMLIPLVFGLLGRYNNKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQY2</accession>
    <name>U5VQY2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358914.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39383">
        <property type="protein sequence ID" value="AGZ39383"/>
        <property type="gene ID" value="AFR_05470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1109"/>
    <dbReference type="eggNOG" id="ENOG5031V4M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204396_0_0_11"/>
    <dbReference type="OrthoDB" id="1896440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="12433" checksum="EE5079805BBF4155" modified="2014-01-22" version="1">MVIADQAVPADIVDRLLWDDAQQMLGRHAEAGHDGNCVWCGWRWPCAPRRLAERAELASRRPWREAWTLRHDLNSVREMPGIRTGLDDNRIRRGTPKIDDLNRGYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRY2</accession>
    <name>U5VRY2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative trypsin-like serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357375.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00710"/>
    <dbReference type="EnsemblBacteria" id="AGZ38431">
        <property type="protein sequence ID" value="AGZ38431"/>
        <property type="gene ID" value="AFR_00710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.143"/>
    <dbReference type="eggNOG" id="COG5640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_5_11"/>
    <dbReference type="OrthoDB" id="1468733at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665685" evidence="1">
        <location>
            <begin position="20"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="34"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="22873" checksum="6D1EBAE956DCBC57" modified="2014-01-22" version="1">MRIRIVVASLATALTGVLAAPAAANAVEAGPDPIIGGSTVASAPWAAAVFSNGSFTCSGTIISANYVLTARHCISGSMSVRVGSVNRSSGGVTRTVSSTSTQNDLALMRLSSSVSTSYMPLSSAYPPVNSTNSLYGWGQTCYSGCAASTTLKTASVRVTSTNGTDAYGGRAIATTAINGNAWRGDSGGPQVYNGAQVGVASTADGQSRQYYGSVAYNRAWISSVAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRY8</accession>
    <name>U5VRY8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00735"/>
    <dbReference type="EnsemblBacteria" id="AGZ38436">
        <property type="protein sequence ID" value="AGZ38436"/>
        <property type="gene ID" value="AFR_00735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.148"/>
    <dbReference type="eggNOG" id="COG3255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123202_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041629">
        <property type="entry name" value="SCP_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17844">
        <property type="entry name" value="SCP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP_3" evidence="1">
        <location>
            <begin position="15"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="110" mass="11822" checksum="4EF27DA3D921D9EC" modified="2014-01-22" version="1">MTSALDALDQGDEPERSVLRDAVRALLTELARTAPGRSVEVRIPPFGAIQCVAGPRHTRGTPPNVVETDPMTWLLVATGRLAWADAVESGRVRASGIRTDLTPYLPVHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS53</accession>
    <name>U5VS53_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase sigma factor sigX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_06690</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359158.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06690"/>
    <dbReference type="EnsemblBacteria" id="AGZ39627">
        <property type="protein sequence ID" value="AGZ39627"/>
        <property type="gene ID" value="AFR_06690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1367"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_5_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="11"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="101"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="19063" checksum="0972D3044D25353D" modified="2014-01-22" version="1">MDAEEAVRQAYEVHYRRLVAQVFGLIGDLAEAEDAVQEAFAKVLAAPRSFVQADDSERWLRVVALNVARSRYRRRWVFDRLVRAGRVEIAPAAVPGLSADRVALLTALRQLAPPTREAVVLHHLADLPVDEVAATLGVPVGTVKARLSRGRATLARFLTDDDPAPLTEEVRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSM1</accession>
    <name>U5VSM1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkylhydroperoxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359343.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07615"/>
    <dbReference type="EnsemblBacteria" id="AGZ39812">
        <property type="protein sequence ID" value="AGZ39812"/>
        <property type="gene ID" value="AFR_07615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1552"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_6_0_11"/>
    <dbReference type="OrthoDB" id="1893149at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="146" mass="16295" checksum="28950099A9F8E196" modified="2014-01-22" version="1">MPRLTIDRVAPGAYRAVYGMEKYCRENVDPTLYELVKLRASIVNGCSFCTDMHSREALAQGESSRRLFAVAAWHDAPFFSEKERAALALTDAVTRLGEHGVPDEVWEPAAKAFTEEELGNLLMAIATINVWNRLSIATRNEPPLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSM2</accession>
    <name>U5VSM2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyl-accepting chemotaxis aspartate transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_08495</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359519.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08495"/>
    <dbReference type="EnsemblBacteria" id="AGZ39988">
        <property type="protein sequence ID" value="AGZ39988"/>
        <property type="gene ID" value="AFR_08495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1729"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_431914_0_0_11"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009875">
        <property type="entry name" value="PilZ_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07238">
        <property type="entry name" value="PilZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="301"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="388"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="689" mass="71988" checksum="B80AD31A45CA6BF6" modified="2014-01-22" version="1">MSLTSAAPPALLRPVLPLADRMRTSMRLGVLILVLMIPGIVATCGYVLQARSKIAFSAAERDGLEVVRPALLAMASTVAAKEPDLAAVRDAVGRNPELRLEDSLRAVPQGLPSTPVQRFALATALAGLITDAGNNSNLILDPDLDSFYVMDAQIVQLPKALLAAAKIAAAEVTSGNSAVAEQAVRAGELAGAASSLTYDVDTADANTKLAGLAARLEPVGRAADAASGLAAQLTRSLERPGPADVGPLATALAAAVGPLVEVLDDLLEIRVTGFANERLIVLLVTIGGFVLAAWFAAGVLWRTRHDVALAVQGVTAIADGDLEPRPLPAGRDELGDLGQALTSTRSRILAQEEEIAKSQAVREEQLRVSFQHQRQAEMRLRDRAQTIIDESTSVIAEELRLVTSQVGDVREAADTIDSGISATDAATEAVVAHARRAENVITSLEQSLRRVADTATLVKGIAGQTRLLALNATIEAARAGELGLGFTVVADEVKELATSTSTSTEQIAATIAELERDTAQMAATIAEMVSGIAGVGEAATSLRAVAADQGTVVGRLADQMGQTIGRVEEMSGLSAQLERREADRVTVTGSIRVRRGNEVLTPTLINLSRGGVRVQLQPGTRLNVGDAVEIDDLGPAGDPIRVQARVVSLGTGEEHDEAGLQFMIADNGTAERVDRYVSNLVADASVEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT24</accession>
    <name>U5VT24_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-methyltransferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_08625</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08625"/>
    <dbReference type="EnsemblBacteria" id="AGZ40014">
        <property type="protein sequence ID" value="AGZ40014"/>
        <property type="gene ID" value="AFR_08625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1755"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005533_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031725">
        <property type="entry name" value="ASMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="O_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="PTHR11746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16864">
        <property type="entry name" value="Dimerisation2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Dimerisation2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransf_2" evidence="3">
        <location>
            <begin position="100"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="35508" checksum="BAB7E37A3084CE2B" modified="2014-01-22" version="1">MDLVTGFWSFKTFAAAVELDLFSRLADGRALTVAEAEQELGLPERPADLLLTACASLGLLERAGDGYRNSALAEEFLVRGRPYYFGGQVSYCDQRTYLPWHRVGEALRTDRPLTWDPATQESMFTTADPQLLATFWEAMYSTSIFTARALAGAVDLGAHRRLLDVGGGSGAYPIELCRLNPGLTATVLDLPHVCEIAAGKIELAGLGDRITATPGNFLTDAALPGGHDVLLFSMILHDWDEPTNRELLAKAYAALPAGGLVIISELLLDADRTGPAPAALMGMNMLVETVGGRNYSDAEYAGWLTGAGFTQIRTVRFDAPGANGVVLGIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTV8</accession>
    <name>U5VTV8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative DNA alkylation repair enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40438">
        <property type="protein sequence ID" value="AGZ40438"/>
        <property type="gene ID" value="AFR_10745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2190"/>
    <dbReference type="eggNOG" id="COG4912">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079880_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014825">
        <property type="entry name" value="DNA_alkylation"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08713">
        <property type="entry name" value="DNA_alkylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="24787" checksum="B7A72690F47B587B" modified="2014-01-22" version="1">MERLVRVFEAARDPERAAGAAAYMRDQFAFLGLATPAQRALARTVVAGLGAPAEGDLHKVALACWEREEREFQYFACDWLRTHVAVPGPGFLATLRTLITTKPWWDTIDPLATRVVGGLVRRHPQLSADMDAWSADRDKWLVRTAILHQLHYGVETDTERLFTYCTRQAGHSDFFVRKAIGWALRQYARTDPDAVRRYVNRNRDALSPLSIREATKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTY1</accession>
    <name>U5VTY1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase</fullName>
            <ecNumber evidence="6">6.3.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_10845</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine</text>
            <dbReference type="Rhea" id="RHEA:28374"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:83905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.2.10" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360349.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10845"/>
    <dbReference type="EnsemblBacteria" id="AGZ40458">
        <property type="protein sequence ID" value="AGZ40458"/>
        <property type="gene ID" value="AFR_10845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2210"/>
    <dbReference type="eggNOG" id="COG0770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031507_0_0_11"/>
    <dbReference type="OrthoDB" id="1861122at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047480">
        <property type="term" value="F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008766">
        <property type="term" value="F:UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005863">
        <property type="entry name" value="UDP-N-AcMur_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01143">
        <property type="entry name" value="murF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="7">
        <location>
            <begin position="32"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="9">
        <location>
            <begin position="107"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="8">
        <location>
            <begin position="312"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="460" mass="46769" checksum="878408A18B74466C" modified="2014-01-22" version="1">MIPLTLADIAAAVRGTVCDGEPGTIVTAPVRHESARVEAGGLFAAFAGARVDGHTFAAEVIAAGAAAVLASRPVGVPAVVVDDVRDAYALLAAEVVSRLPNLHRIGVTGSSGKTSAKDLLGRVLGRLGPTVAPPGNPYVPETVLLATEETRFLIVELGARRVGDIAALMPVVRPTVGVVLGVGSAHLGTFGGRDAIVAAKGELVEALPETGLAVLNADDAAVTAMAARTGARVVTFGRHRSARVQAERVVLDGRARARFQLRTPVGTAEVALRLHGEHFVTAALAAAAVALEYTHDVWLIADALSTAAAVSPGRMRLTDRADGVTVLDDSYSADPESMAAGLRTLTALAAGRRRTVAVLGQMTELGDASAAAHREIGRTAARLGVDVVITVGGSDAQTLAAAAGPDAVHVADGEAAAERLDDVLQPGDVLLVKGSNVAELAAGYSVRSAPPSTGRVSPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU50</accession>
    <name>U5VU50_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotide-binding protein AFR_10550</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Displays ATPase and GTPase activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RapZ-like family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10550"/>
    <dbReference type="EnsemblBacteria" id="AGZ40399">
        <property type="protein sequence ID" value="AGZ40399"/>
        <property type="gene ID" value="AFR_10550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2151"/>
    <dbReference type="eggNOG" id="COG1660">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059558_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00636">
        <property type="entry name" value="RapZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005337">
        <property type="entry name" value="RapZ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30448">
        <property type="entry name" value="PTHR30448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03668">
        <property type="entry name" value="ATP_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005052">
        <property type="entry name" value="P-loopkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="82"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="33711" checksum="20A892C43340C99F" modified="2014-01-22" version="1">MQEECVTESMPEFAPEPTPDEADTDLVVVTGVSGGGRSTVARALENVGFYVVDNLPQALMLDMAQLAFEAGGAARRTAMVLDVRSRAFSTDLAGAVRALKDRGFSPRVVFVDADDEVLIRRFESVRRSHPLQGDGRLADGIAAERKLLEEAREQSDVIIDTSHLNVNQLRRRVEELFGGEDARRLRITVLSFGFKYGLPPDADYVLDARFLPNPFWVPELREHTGLEEGVSGYVLGQEGAHDFVATYASLIATTAPGFEREGKRYLTVAIGCTGGKHRSVAIAEELTAQLRDIRLSAHSSHRDLGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU65</accession>
    <name>U5VU65_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="5">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">trpA</name>
        <name evidence="7" type="ORF">AFR_10315</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360146.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10315"/>
    <dbReference type="EnsemblBacteria" id="AGZ40352">
        <property type="protein sequence ID" value="AGZ40352"/>
        <property type="gene ID" value="AFR_10315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2100"/>
    <dbReference type="eggNOG" id="COG0159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_0_0_11"/>
    <dbReference type="OrthoDB" id="912786at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="PTHR43406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="27751" checksum="D41E0B5D652005D7" modified="2014-01-22" version="1">MSISETFGKAKAEGRAVLVGCMPAGFPTVDDSIKSMVAMAEAGCDVIEVELPYSDPVMDGPVIQKASDIALAAGVRTKDTLRIIEAVANAGATVVLMTYWNPVEKYGVDAFARDLAAAGATGMITPDLIPDEAQEWIAAADRYAIDRTFLVSPSSTDDRLAMTVNQCRGFVYATALMGVTGARTQVSSQAPELVERIRAVDPEMPVGVGLGVGTGAQAAEVAAFADGVIVGSALIRCVLDAPDRGTGLDRLRALSAELAEGVRAARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU80</accession>
    <name>U5VU80_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane-bound lytic murein transglycosylase F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_10415</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360184.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10415"/>
    <dbReference type="EnsemblBacteria" id="AGZ40372">
        <property type="protein sequence ID" value="AGZ40372"/>
        <property type="gene ID" value="AFR_10415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2122"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_18_1_11"/>
    <dbReference type="OrthoDB" id="847343at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665719" evidence="5">
        <location>
            <begin position="29"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="6">
        <location>
            <begin position="64"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="31311" checksum="555979C920C26DDF" modified="2014-01-22" version="1">MFRTTPLRRAFVGLTAALAMTVSLSACGEESGDTGTGSGAPVPSASADTSLADKVPAEIKTAGKLIIGTDSTYAPNEYLDADGKTVIGFDVDLFNAVAQKLGLKTEWQSSKFDAIIPGVTSGKYNIGVSSFTVNAEREKQATMVSYFSAGTQWAAKAGATINPDDACGKKVAVQVGTVQLDDITARSKKCTDGGKAKITIDQYQSQADATNAVVSGKDDAMLADSPIGAYAVKQTNGQLALVGEIYDAAPYGYVLPKAQADFGAAIEQALEALIADGTYKTILSKWGVEAGGITDPAVNPQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUF4</accession>
    <name>U5VUF4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360581.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11470"/>
    <dbReference type="EnsemblBacteria" id="AGZ40583">
        <property type="protein sequence ID" value="AGZ40583"/>
        <property type="gene ID" value="AFR_11470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2338"/>
    <dbReference type="eggNOG" id="ENOG5032CP5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195022_0_0_11"/>
    <dbReference type="OrthoDB" id="1983596at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="79" mass="8600" checksum="8D2649DD02528DC1" modified="2014-01-22" version="1">MTAIHTDDVRTEALFVSDLQRSQQPTAELIREAVSETVGRLGENGCAALVAQEFGEHPDCALGRMQWARTAVRRAFAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUG8</accession>
    <name>U5VUG8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358901.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39370">
        <property type="protein sequence ID" value="AGZ39370"/>
        <property type="gene ID" value="AFR_05405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1094"/>
    <dbReference type="eggNOG" id="ENOG502ZUG8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161236_0_0_11"/>
    <dbReference type="OrthoDB" id="1999511at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="10698" checksum="149D978197D022CE" modified="2014-01-22" version="1">MTAGTFDTAVQRLIGQVNHWETTRWRPHGDAVFALVQSLADHAADAENRRRRPVPRLADTVLPDQLRVMADDLLAASPSPEVLTTATEAVTTIRRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUM2</accession>
    <name>U5VUM2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative formyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358877.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05285"/>
    <dbReference type="EnsemblBacteria" id="AGZ39346">
        <property type="protein sequence ID" value="AGZ39346"/>
        <property type="gene ID" value="AFR_05285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1069"/>
    <dbReference type="eggNOG" id="COG0223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008537_1_0_11"/>
    <dbReference type="OrthoDB" id="178084at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016742">
        <property type="term" value="F:hydroxymethyl-, formyl- and related transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009188">
        <property type="entry name" value="NiFe-hyd_mat_HypX/HoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006787">
        <property type="entry name" value="Hydrgn_mat_HoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_C" evidence="2">
        <location>
            <begin position="177"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="597" mass="63991" checksum="484519105AB95DEB" modified="2014-01-22" version="1">MRVLLLVSAFNGLSQRVWCALREAGHDVGVLVATGHEDIVDGVAAAQPELIICPYLKDRVPAEVWKHHRTVIIHPGPVGDRGPSSLDWAILEGAPTWGVTALQAVEELDAGPIWATRTFPMPAAAPRKSSLYNGPVADAAVECVFEVLTKAADPAFRPVPATELTIEVQGARPRPAMKQADRAFDWTQPSEHIIRRIRAADGNPGVRTTLAGLDVFAYDVHPGLTRGARPGTLLGRRQGAVLVGTGDGSVWLGHLRDATQTRPVKLPATTLLGKRLRGVPNSPVPVGTEPEGPAYRQVRYRRSGPVGWLAFDFYNGAMSAGHCRRLLAAFRHAAAQDTRVLVLRGGTDTFSNGIHLNVIDASGDPADAAWANIKAINEVCREIITCTRQVVIAAYAGSAGAGGAMLGLGADIVAARQGVVLNPYYDMGLYGSELHTFTLPRRVGPETAQRLIDAKLPVSAEQAADLGLVDEVGPRHPEAYGEWLTALAVRQADAREARRRQAAKAKRLAGERVPLDAYEARELAEMSSDMFGDRSGFAAARRAFVGKVRPTTTPAHLLPAPRSEQPAARPRQLSRPRNTAPAGDTQVRVRSAVPMSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVB5</accession>
    <name>U5VVB5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359087.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06335"/>
    <dbReference type="EnsemblBacteria" id="AGZ39556">
        <property type="protein sequence ID" value="AGZ39556"/>
        <property type="gene ID" value="AFR_06335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1285"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_1_11"/>
    <dbReference type="OrthoDB" id="1367386at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="14"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="30410" checksum="095D314E67BEC910" modified="2014-01-22" version="1">MKQRTIGNVQVGAIGLGGMPMSIEGRPDTDRSVRTIHAALDAGVTLIDTADAYHLHADEVGHNESLIAQALSSWSGDTSGVLVATKGGHLRPGDGSWTVDGSPAHLREAVEGSLKRLGVDAIGLYQFHRPDPGTPYEESVGTIRDLLDEGKIRLAGISNATVDQIRQAQEILGGRLASVQNQYSPAFRSSEPELRLCDELGIAFLPYSPLGGIARAGELGDRFSEFARIAEIHGVSPQRVTLAWMLSKSEHVIPIPGASRPESIIDSAQAADLTLTAQDLVQLDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVC6</accession>
    <name>U5VVC6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_12715</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360888.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12715"/>
    <dbReference type="EnsemblBacteria" id="AGZ40829">
        <property type="protein sequence ID" value="AGZ40829"/>
        <property type="gene ID" value="AFR_12715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2582"/>
    <dbReference type="eggNOG" id="COG4977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032783">
        <property type="entry name" value="AraC_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037923">
        <property type="entry name" value="HTH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12852">
        <property type="entry name" value="Cupin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51215">
        <property type="entry name" value="SSF51215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="201"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="32621" checksum="E154E4104474B9EE" modified="2014-01-22" version="1">MDLFSDVLEVSGVRGTIGARIEAGGSWSVPVVGCTAAVLHVVIAGTAWLGLEDRSSPWLELAAGDVVLMPGGPSHVLGSAPGSGAAVAALAFESNEVIRLGLPPTRTRILTIRYDCDHSVNTQILGALPQVVHIRGDQGEAGFDDTVRMLGRELSQPRLAGRAVLNSLVDILLVQLMRAWLPTRPGHRGTWLGVLEDPLVHSAMERLHAEPARPWTTTTLASALDVSRATLSRRFPAAIGQSPAAYLTQWRMDLAAVRLNKTQEPVESVAAAVGYQSVPAFSRAFARSHGATPGRYRAARRDGARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVM0</accession>
    <name>U5VVM0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359212.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06960"/>
    <dbReference type="EnsemblBacteria" id="AGZ39681">
        <property type="protein sequence ID" value="AGZ39681"/>
        <property type="gene ID" value="AFR_06960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1420"/>
    <dbReference type="eggNOG" id="ENOG50313FX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101008_0_0_11"/>
    <dbReference type="OrthoDB" id="2013286at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="23689" checksum="2ADF5799684ADB4A" modified="2014-01-22" version="1">MSPTVVIILIVVLLVIAAAVVLGARASRRKQLQNKFGPEYDRVVGDADSRSEGERELREREKRHAEFELKALSAESKAKYSVAWEEVQIQFVDSPEQAVSTADELVTKLIAERGYPTGDYDDQLANLSVAHARTLEHYRDAHAISERSKSGEAETEDLRQALVHYRALFADLLGENPIRSEGAASPASEPKVPEQKINNHDSDVANSRPDATSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVW0</accession>
    <name>U5VVW0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_07855</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07855"/>
    <dbReference type="EnsemblBacteria" id="AGZ39860">
        <property type="protein sequence ID" value="AGZ39860"/>
        <property type="gene ID" value="AFR_07855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1604"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="32654" checksum="6C1E4724D0AFA731" modified="2014-01-22" version="1">MVCVLSIALGTRNVALSTVWDAFVNYADTDDQSIVRDLRVPRTILGLLAGVAFGLSGAVIQAVTRNPLADTQILGINAGAGLFVVFAVGVLGLTSLSQYMWFAFAGVAVALTLVYALGSAGRAGATPVRLTLAGVALGAVMDGISGGIRLVRPRAFDYLRFWDIGSLAGRPVDVATTILPFVAAGVVLALIVARSLNAVALGDDLARTLGANIGRTRVLATVSVMVLAGAATAAVGPIGFVGLMVPHVVRWFVGPDQRWIFVYTIVCGPILLLLADITGRLVVRPGELRVGVVTAFVGAPVLIWLVRRSRVSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVZ5</accession>
    <name>U5VVZ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_13455</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361036.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13455"/>
    <dbReference type="EnsemblBacteria" id="AGZ40977">
        <property type="protein sequence ID" value="AGZ40977"/>
        <property type="gene ID" value="AFR_13455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2732"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_2_11"/>
    <dbReference type="OrthoDB" id="1875458at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="9"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="24740" checksum="BB359D869639FAC6" modified="2014-01-22" version="1">MPGPNFDRSALSTRVTELFRSQVTDGDWPLGSKIPTESELVEWSGAGRNTVREAIASLVQGGILRREQGRGTFVVSTSDLRSSVSRRAAATSRRDSLELRLALDAASARIAAVRRTEADAERLVTLLNERRQAWTGGDARERVRADSALHRAVVGATHNAMLQDVYEGLLGVFEEVQLHDVAGETDALAQLHADLVEAIVDRDPERAAATMSELLQPMIESAEPSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW38</accession>
    <name>U5VW38_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_14580</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361257.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14580"/>
    <dbReference type="EnsemblBacteria" id="AGZ41198">
        <property type="protein sequence ID" value="AGZ41198"/>
        <property type="gene ID" value="AFR_14580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2961"/>
    <dbReference type="eggNOG" id="COG4977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_0_11"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032783">
        <property type="entry name" value="AraC_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037923">
        <property type="entry name" value="HTH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12852">
        <property type="entry name" value="Cupin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51215">
        <property type="entry name" value="SSF51215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="194"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31926" checksum="BA70E55771C1C3CC" modified="2014-01-22" version="1">MDMITATISSARIGRANGRRVAEANAYGVRFPKFPVLGFHVMLSGEGWLITQSAPPVAIRPGDVVFTASGAEFGLARSPRALAELPPIVLADVPPPPAPVEFEFLCGGYPLEFGKVPGFLRHLPEVVTFTPDYERHPQLQALVEMLHSDYTEPGPGTGAGRSALIDLMLVHILRHLQEHGRLAGWPPNTDPGIADALHEIHTRPEHKWTVQELSEVAGMSRTAFNRRFTASVGTSPMAYLINWRLDMGAGLLRRSDVPLAGIARKVGYSTEFAFAAAFRRRYNVSPGRFRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW55</accession>
    <name>U5VW55_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08380"/>
    <dbReference type="EnsemblBacteria" id="AGZ39965">
        <property type="protein sequence ID" value="AGZ39965"/>
        <property type="gene ID" value="AFR_08380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1705"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="172" mass="18213" checksum="79DD18BE896CA9A5" modified="2014-01-22" version="1">MQQWMSVDGFLAGPGGEGDVFAAVADFSASEAHNTTLLASVDEVLLGRRTYEAFREFWPGAHDEPMAQLVNTLPKTVCTTTLTEAPWGTHAPARIVPDAVAHVRELQGTAIVWGSVALMGALLAAGQVDEVELLVAPIALGGGTPLPAIPKLRLLETETWPASVIRTRYAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWI8</accession>
    <name>U5VWI8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-1,2-mannosidase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_14325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361206.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14325"/>
    <dbReference type="EnsemblBacteria" id="AGZ41147">
        <property type="protein sequence ID" value="AGZ41147"/>
        <property type="gene ID" value="AFR_14325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2907"/>
    <dbReference type="eggNOG" id="COG3537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003690_0_0_11"/>
    <dbReference type="OrthoDB" id="100818at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005887">
        <property type="entry name" value="Alpha_mannosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041371">
        <property type="entry name" value="GH92_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012939">
        <property type="entry name" value="Glyco_hydro_92"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07971">
        <property type="entry name" value="Glyco_hydro_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17678">
        <property type="entry name" value="Glyco_hydro_92N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01180">
        <property type="entry name" value="aman2_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665823" evidence="2">
        <location>
            <begin position="24"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="3">
        <location>
            <begin position="1594"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1109"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1109"/>
            <end position="1130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1650" mass="172984" checksum="45922C7C1B416BBD" modified="2014-01-22" version="1">MRSALAIAVVIAGIPLGAAPAQAAPADRPAAPAFQSSFEQADPPSLDSTPFGKQTNVTGRIPPGSLSAFAAGVTASAENPPGETATRLLDGNSGTKWLAFARTAEIVYRFDRPVVATDYAMTSADDVPGRDPKNWTVDGSTDGTTWTPIDTQADQAFSDRRQEKKYKTTNTTAYTSYRLSITLNAGEAITQLADWDLRDGRPGEAPMLATVAGGPASGLNSKPGVGFTGTRALRYGGKHVADGAAAASDVLFDKVGRRLTNRSELSYLIFPEGSGDLAVPSSWVALDLVLDNGSKLSTRNRVVDSNGFGVSARDHGQQKSLFENQWNAIHIDLSSLAGRTVEKVLLSYDYPGGKASTVFSGWVDDVRIGTRAPGIDGSNRLNYVDTRRGTLSSSAYSRGTNVPATAWPNGFNFFTPMTDGDSQGTLYEYQRANNADNLPTLEAIGISHEPSIWMGDRNQLGIMPSLSATPTGDLAARALPFRHADEIARPDLYSVKFQNGLTTQVTPTDHGGVYRFQFPGTTGSVVVDRVGGSASLNVAADGVVSGWVDGGNGSGISRMFVYGAFDTTPTTVGTATGGRTDARYASFATKPGQEVLLRLATSFISLDQAHRNLDLEVTGKSFSAVNRAAAVAWNDRLDVIDVEGATQNQLVTLYSNLYRLNLYPNSQFENTGTAAAPKYQHASPVAPQTGAATGTTTNAKIVDGKIYVNNGFWDTYRTVWPLYSLLYPKLAAELVDGFVQQYREGGWIARWSSPGYSDIMTGTSSDVAFADAYLNGALPTATALEAYDAALKNATAVSPSNNVGRKSLDKSIFLGYTPDSQGESVSWGLEGYINDHGIGLMAAELAKDRKTPAARRHQLTEESAYFLDRAKNYVQMFDPKTGFFRPKTAAGAVAGGANFDPTAWWGPYTETNGWNFAFHAPFDVDGLESLYGGSAGLVKKLDQFFATPETGGGGTIHEMVEARAVRMGQLGMSNQPSHHIPYLYAAAGEPSKTQAAVREIQQRLFVGSEIGQGYLGDEDNGEMSAWSIFSALGFYPLSLGSGEYTVGSPLFKKSTVHLENGRKLVISAPGNSTDRRYISSAELNGKKLRTATLPVDALTGGGRVEFTMSSTPTRWGQRTTPAQPQRTPGTDATKAGYGVLSVSDGSSLAPLTDDTSRTATTFATATPAITWKSTSGAVTVGSYTLTSGSTGSAPTSWRLEGSADGTTWKTLDTRTAEAFRWPMQTRPFQISRPRPYKVYRLTLSGPAPATLAEVELLAGPGSTGELAITPATGLQAVPGAAVTTALATVSGATSATATADFLDGKGPQPATLTRTPLGTQTVSAEHTFPNSGEYSVVVAASEGKSKVSTTVVVSVGRDETLTGAFDSACIGDPGVGADCDAGGWAFNRKLLADSGLVQGKTIAVPGTELTFDLPNATPKGLDNATANGQTIRLAPGSDATQLSIIGTATQATQHITATLRFSDGSTAPVAIDYGDWVGAAGNPISGNIVVGKSAGRLNGASDSDGQTVAIFSTAPYTIPAGKTPVSLELPTQTGDPKAVGRVHVFAIASDGRRTAEKALSATPVPVGTQRAGADFTAKVATASGGTATYTARINWGDATALQTATVTVDDAGQATVTGSHRYARPGNYTVAVTVDDGVTSAVTTTSVTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWP2</accession>
    <name>U5VWP2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361271.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14650"/>
    <dbReference type="EnsemblBacteria" id="AGZ41212">
        <property type="protein sequence ID" value="AGZ41212"/>
        <property type="gene ID" value="AFR_14650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2975"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047930_1_0_11"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035418">
        <property type="entry name" value="AraC-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14525">
        <property type="entry name" value="AraC_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="225"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="36278" checksum="7548635A181EB15E" modified="2014-01-22" version="1">MTEESIHPGTHWQRFATTDPDEGYDHIRKAYVDFSVRSSNDRGEGFRLRTVATELGDVGLSRMVYSAHTFVETVPEGDLNVSRAIKGRYSLIHGKEEHRVLPGEVFLILPDHSLGTDFEDVDVFTTRLPRALLEEAAHTPAGFNGADLRFDSTQPISAQLGRHWSATVSYVTQSILSEPSLRTNPLIIAEARLLLARTALAAFPNTSLDARPTSTGGDVRPHGLRRALAYIDDNAGRPISVAQVAAATGLHPRSLQLAFRRHLGTTPTNYLRRVRLEHAHRDLRNADPATGVTVTMIANRWGFPHLGRFSADYRRAYGFSPSHTLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWS1</accession>
    <name>U5VWS1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_14800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361301.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14800"/>
    <dbReference type="EnsemblBacteria" id="AGZ41242">
        <property type="protein sequence ID" value="AGZ41242"/>
        <property type="gene ID" value="AFR_14800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3005"/>
    <dbReference type="HOGENOM" id="CLU_1458346_0_0_11"/>
    <dbReference type="OrthoDB" id="1677446at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="20574" checksum="D197FB8927D50A4B" modified="2014-01-22" version="1">MIDRRKRPLLPRGERVLISEHDTAGDRQVVGTRYAVYHQGAPHEPEPWHRLGWDQIERVDRDSARGELVLVPAVPGRVPVLVLRIRDGQRFHDLARERIAATTLLRVPLSRSGRPAGALTARRRADGRGEVLWVLSCGGETPWTTGELTPVVRQACVQAGLPTTAVVPRIVHGCRTRNPLPPGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX84</accession>
    <name>U5VX84_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_16405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361638.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16405"/>
    <dbReference type="EnsemblBacteria" id="AGZ41563">
        <property type="protein sequence ID" value="AGZ41563"/>
        <property type="gene ID" value="AFR_16405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3327"/>
    <dbReference type="eggNOG" id="COG2130">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037224_1_0_11"/>
    <dbReference type="OrthoDB" id="1301821at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021276">
        <property type="entry name" value="DUF2855"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11017">
        <property type="entry name" value="DUF2855"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="355" mass="38649" checksum="F5D74C25C57BD8A0" modified="2014-01-22" version="1">MPASWTFAVARDDLGRTTLTEGETLALADGEVLLRVDRVGLTANNVTYAVLGEVMRYWQFFPPEPRGLTGEWGLPPLWGFAEVAESTVPGVETGLRVYGYLPPAGHLVVRPDRVDASGFRDASEHRAALPSPYNAYRSTGNDAAYRKDQEDLLILFRPLFFTSFMLADQVADNNYYGASALVLSSASSKTAYAAAFELRGRGPRLIGLTSPGNVEFTRSLGCYDEVRAYDAVDGLDVVPTAYLDLSGAAATRTALKERLGDHLVRDVAIGLTNQVPNAADAGEVFFAPTQMRKRSEDWGREGLDDRFAEAWQRFAAVVEGWIDIRVSTGPEALQAAWLEVLAGGTPPRVGQIVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXB4</accession>
    <name>U5VXB4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Spermidine/putrescine import ATP-binding protein potA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_10405</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10405"/>
    <dbReference type="EnsemblBacteria" id="AGZ40370">
        <property type="protein sequence ID" value="AGZ40370"/>
        <property type="gene ID" value="AFR_10405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2120"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="47"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="79"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="32555" checksum="D5C22C4BAF0F3704" modified="2014-01-22" version="1">MSEPHKETPAELDAENARPVDQAAPTTDEVAAPSKTAEAKPEGEVLLEVDNVTLRFGGVVALNEVSFTLRKGEIFGLIGPNGAGKTTCFNAMTGVYRPTSGGIRFKGQSIVGKKKHEITRGGIARTFQNVRLFPEMTALENVMVGADAHHKTSVIAALFRLPRHHREERTGRDRSYQLLRFVGIENRASDVARNLSYGEQRRLEIARALATNPTLLCLDEPAAGFNPAEKQELLALIRKIRDSGVTVLLIEHDMRLVMGVTDRIVVLEFGKKIAEGTPAEVRDNPAVIAAYLGVPTDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXP9</accession>
    <name>U5VXP9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41758">
        <property type="protein sequence ID" value="AGZ41758"/>
        <property type="gene ID" value="AFR_17400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3527"/>
    <dbReference type="eggNOG" id="ENOG50333YK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125494_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="17871" checksum="2D45E090948B5937" modified="2014-01-22" version="1">MTDPMTPDDGLLTRLGAVLAGAEEPPAEVLELARQSFVLRELDAELALLVEDSHGPAASVRSVAVRGSAREPRQLTFQVVDAQGRDELIIAVQVESVGRHRRLTGHLAPQGPAHIEVRQPAVPQARGVDADLRGLFVIDDVLPGPMRLTCRRADWPAVATQWTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYG0</accession>
    <name>U5VYG0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362107.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17695"/>
    <dbReference type="EnsemblBacteria" id="AGZ41817">
        <property type="protein sequence ID" value="AGZ41817"/>
        <property type="gene ID" value="AFR_17695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3587"/>
    <dbReference type="HOGENOM" id="CLU_1830814_0_0_11"/>
    <dbReference type="OrthoDB" id="869726at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15132" checksum="C8A60916468BEFF8" modified="2014-01-22" version="1">MAGFIVLEDGRSYAASGRATDAVIRAIAAELADPVFAEWLTGCQAQFLGSGMGGVDVRQIAPQHRGPFYEAARAAFGRARDNGFEHMEPGSDELATWLSNFGDLIEMVDRWRAGEAPELFNPHVAGLEPPTDRREGPGWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYG8</accession>
    <name>U5VYG8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DSBA oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362401.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42028">
        <property type="protein sequence ID" value="AGZ42028"/>
        <property type="gene ID" value="AFR_18760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3809"/>
    <dbReference type="eggNOG" id="COG2761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069253_0_2_11"/>
    <dbReference type="OrthoDB" id="996965at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DSBA" evidence="1">
        <location>
            <begin position="6"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="216" mass="23513" checksum="4292E22415F45EF9" modified="2014-01-22" version="1">MAPVLVEVWSDIVCPWCYIGKRRLQTALGTFEHADDVEVVWRSFQLDPSHPKGFAEPVYDHLAKKFGGSLAQVKGMTSQVGALAAAEGLDYDFEHAVSVNTFDAHRLIHLANTHGLGAEAHERLMRANLIEGETLDTPTLVRLGAELGVPAAEAERVLAGDEFTEDVNDDIRQAQMYGATGVPFFVLNRAYGVSGAQSSDVFLQALRTAYESAPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYG9</accession>
    <name>U5VYG9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12020"/>
    <dbReference type="EnsemblBacteria" id="AGZ40691">
        <property type="protein sequence ID" value="AGZ40691"/>
        <property type="gene ID" value="AFR_12020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2447"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_942100_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="2">
        <location>
            <begin position="83"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="31158" checksum="18C5ECD8B0CCEB3B" modified="2014-01-22" version="1">MKAVNPGGNDDVLRLIAETCREAAAGNFEVRLPVLGDSPDAQAARDELNALLDITDAFVRESGAALGAAAEGRYHRRFLTRGMRGSFRKAAAQISNSGEQMSVAAGRVAEAASARVALADELESAVLTVSEQVATAATEMGASANGLADFAREAVAEAERGLQTVTSLRTASSQIRHAVDLINQVASQTRLLALNATIEAARAGDAGRGFSVVAGEVKTLANETSSSSEEILGQVNTVQAAAADAVSVLETVTNSIREMSGLVQGIATAVDTGDHSGTAGLSQLAEVLRGEVHRFVSTARQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYT7</accession>
    <name>U5VYT7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_19230</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362490.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19230"/>
    <dbReference type="EnsemblBacteria" id="AGZ42118">
        <property type="protein sequence ID" value="AGZ42118"/>
        <property type="gene ID" value="AFR_19230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3906"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_11"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665361" description="Histidine kinase" evidence="7">
        <location>
            <begin position="29"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="182"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="242"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ42118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="450" mass="47850" checksum="8AC0ABCDFB732E7C" modified="2014-01-22" version="1">MRRAGLRVRVIAGFAAGALALSATMALATYQVARKSLLGERERTAVRAAYFDASVVRSGLGSTTRPDVSAVLRTLDTGENRRAVLQRDGQWFSSKADVGVTDAIPPLLQRQAAAGTPSVQRVRVDGHASLVVALPLGDNDVFYEVDSLQELEHTLGVLTVVLTLVAVGTAALGALLGWYASRYVLRPLTRVADAAQEIAGGASKYRLDPAAEPDLSRLTTSFNHMVDELSARLERDRRFAADVSHELRSPLQTLAAAASVLERRREHLDERTATAAGLVTDEIARFQQLVNDLLELSRGDRPAARTGTDVADLARQICRDRRVPDTIVEATGDAAWCVDRRRVGQLLGNLLDNAQAYGGGAIAVHLRLDGDVGRIEVDDEGPGVSPEDKPVIFDRFVRGRSAGARADSGGAGLGLALVAQHAAAHGGTAAVQDRPGGGARFLVELPGCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ06</accession>
    <name>U5VZ06_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362457.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19060"/>
    <dbReference type="EnsemblBacteria" id="AGZ42084">
        <property type="protein sequence ID" value="AGZ42084"/>
        <property type="gene ID" value="AFR_19060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3870"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_17_3_11"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="16"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="190" mass="20161" checksum="2184373664EE30DE" modified="2014-01-22" version="1">MDSGGTDTKTMRRDAARSRAAILRALDTMYAEEGSVPGMHALAARAEVGVATLYRHFPTREDLLTALAVDRLVHLVEFADQAGATPDTAQAVRNFFESVAGAGCDPALVAHLSPDSADRASQELLTRFQRGLDHLLERAHADGVIRADAQREHVIALLHGLLATVAHLGDQPGGCDLAVDILARGLAAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZC2</accession>
    <name>U5VZC2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_19175</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362480.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19175"/>
    <dbReference type="EnsemblBacteria" id="AGZ42107">
        <property type="protein sequence ID" value="AGZ42107"/>
        <property type="gene ID" value="AFR_19175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3895"/>
    <dbReference type="eggNOG" id="ENOG503423D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_779937_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="355" mass="35635" checksum="8BB4456FCE5FA740" modified="2014-01-22" version="1">MHSTALLGWFGAALSMTLPWPQVWRSVVRGRTGGLSATACWQGVAMPIGWITYGLLTGESVQIVTNTVTGLTGLSILVMVLVKQGELRTPRKMLISAAGAAGVLSAAAGSAIVAVTTDLGEARVATFLGSVLAITAMLSAVPQPLSLLRDRTQDLAGLSPLRWRLAAAACSCWFAYGLTTGQVAVWLSASVGLSAALIVCWVLWAAGRTPAAAAAPAALTVPAAVTVPAAVAVPARGTARVLRPAYASAAAVRPAVAGARIVRPAASVARSARPVSGPASTPVTAATRGTAATPVAALVPAPGLLTAPALLPAPTGPAAASRRPRNTSRPVRGTARVPGQASWATTVLQGALVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZC5</accession>
    <name>U5VZC5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_19200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19200"/>
    <dbReference type="EnsemblBacteria" id="AGZ42112">
        <property type="protein sequence ID" value="AGZ42112"/>
        <property type="gene ID" value="AFR_19200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3900"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_11_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665376" evidence="2">
        <location>
            <begin position="23"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="12"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="147"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="313" mass="31021" checksum="A3A08B23F31B9E82" modified="2014-01-22" version="1">MSRILTVAAGSAAMTCVGGSVAVSGLLAGAPLFTTQALRYALACLLLVVYARLVRFRLVRPRGAEWLWLGGIAITGLVVFNLALVEGAKHAEPAVIGVAIAGVPLLLAAIGPVLEGRSPRRSVLAGAAVVTVGAALVQGFGRTDGAGLAWAAVAFGCEAAFTLLAVPVLRRHGPVGVSVHTTWLAALMLAVLGPLHEGPSAITRLTGTDLLAGAYLAVAVTAVAFVLWYACVSGIGAARAGLLTGIAPIAAALTGVALGGPAPQASVWLGIAVVATGLAIGLRATPPDRPGPTATITGGPVPLLVGSTAGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZM7</accession>
    <name>U5VZM7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42344">
        <property type="protein sequence ID" value="AGZ42344"/>
        <property type="gene ID" value="AFR_20360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4128"/>
    <dbReference type="eggNOG" id="ENOG5032KA1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099329_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="17717" checksum="B744A0755B8D8EDD" modified="2014-01-22" version="1">MEELHDGEGESVTGCRGLDCAHGDADLGEYPAGFVEAVESEGTGRTADGRYLNWSYDIGYWLDTAPRDTAGRRLEPFVSAAADPGVLPRGTTFRIAGCGRTEDGGRPHATVCAALRDGRWKVTDEFTPGLGGSRHLDLYIGEETGRDFTSSAWYLSLVGVSLLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZY2</accession>
    <name>U5VZY2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerol-3-phosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361339.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14990"/>
    <dbReference type="EnsemblBacteria" id="AGZ41280">
        <property type="protein sequence ID" value="AGZ41280"/>
        <property type="gene ID" value="AFR_14990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3044"/>
    <dbReference type="eggNOG" id="COG2085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076368_2_1_11"/>
    <dbReference type="OrthoDB" id="1768241at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="1">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21083" checksum="5943CE0960A48D83" modified="2014-01-22" version="1">MRIGIIGAGELSGSLAGWWTEAGHDVVVGSSQAGTNTATVTDAAAFGDVVLFAPEWATAHDLIDLTAGALTGKPVLDATNPVVWSETDGYQRAVPDDLSGIETLSAWAPDAHWVKAFNTFPTTVLKRRRGRDPLLAEFICTDHRDARGAASRLIQDAGFAPFFAGRASNAGLTETGGPLQMREVDVADATATFALAVGSNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W065</accession>
    <name>U5W065_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4440 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362965.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21615"/>
    <dbReference type="EnsemblBacteria" id="AGZ42593">
        <property type="protein sequence ID" value="AGZ42593"/>
        <property type="gene ID" value="AFR_21615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4382"/>
    <dbReference type="eggNOG" id="COG4994">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141608_2_0_11"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027843">
        <property type="entry name" value="DUF4440"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14534">
        <property type="entry name" value="DUF4440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4440" evidence="1">
        <location>
            <begin position="5"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="119" mass="13461" checksum="F7F49ECD83281E3E" modified="2014-01-22" version="1">MSEEILAAHRAFQTAELQADTDRLDTLLTDDFRSIGERGYLLDKSAWIGRFTEFGYTDLETGEVDVCLYDRAAILRCVQHSRSVWRGQEMSLTNRVSQVWVGGSGTWQLAGIQFSSHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0I8</accession>
    <name>U5W0I8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bac_luciferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363109.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22340"/>
    <dbReference type="EnsemblBacteria" id="AGZ42738">
        <property type="protein sequence ID" value="AGZ42738"/>
        <property type="gene ID" value="AFR_22340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4529"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_9_2_11"/>
    <dbReference type="OrthoDB" id="1003376at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="367" mass="39039" checksum="748482A1C802F652" modified="2014-01-22" version="1">MPVPLSILDLAPILSGGTAGDALRRTLDLARNAEQFGYSRYWVAEHHFTPGVASSQPALLLGQIAAVTDRIRLGSGAVQTGHQTALSIVEQFGLLDALYPGRFDLGLGRSGQRRQEALKELSSPPPPAEEHVVDGLLIPKPFSFAPLLASERSALLTSLLQQPGAEPPDFPAVLDTIEALLAGTIAEARAVPGEGADLELWILGSSGGQSARVAGERGLPFAANYHVAPAHVLEAVTAYREAFRPSAQLAEPHVMVSADVVVAPDDATARRLAEPHGLWVRSIRTGAGAIPFPTPDEAAAHVWTDADRDLVADRVATQFAGTPADVAERLRTLQRVTGADELLVTTVTYDHADRVHSYELLAKEWLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0P0</accession>
    <name>U5W0P0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">YkuC-like MFS-type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_22095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363060.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22095"/>
    <dbReference type="EnsemblBacteria" id="AGZ42689">
        <property type="protein sequence ID" value="AGZ42689"/>
        <property type="gene ID" value="AFR_22095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4479"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_11_1_11"/>
    <dbReference type="OrthoDB" id="498436at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="15"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="538" mass="57493" checksum="927364D67AC6739C" modified="2014-01-22" version="1">MTVAVSPWAPFRHRAFFWLWLGVVVSSVGLWAQTVGAQWLFVDDPHAATIVTLVQTATTLPMMLLALPAGVLADAFDRRALMFGVQIYFVAVAILLAVLTAAGSMPPALLLTFTFAIGAGQALLSPTWQALITELVPRAELAAATRLDMISVNVARAAGPALAGVVIARWGVPPVFAITAAAAGILTLVLLAWRRPRVNRGEREPFLPALRAGGRYVRHEPVVRRTLVRFAGFLAPACAVWALLPLIASRQLGLGANGYGLLFAALGLGAVAGALGLGRITQHLSSNAVLALAAIAFGLTFGALALTSSLWAAAPLLVVCGFGWTATVSTVISELQLFLPGWVRARAIAIYLMVFLGTQAVFSPVWGQITQHLGLDTAMFAAAALAGLSAIGALTSPVPDNQHLDRSALSYWIPPPVAHEPKPDAGPIVVSIEYQVAPDQEAPFLAAMAAMRRSRLRSGASRWELYRVGEHPDLFTEQFQVPTWREHQLQHDGRLTAEDQAIEDAAFAHIIGTPRTRHLITPVTARNTSSQRSPDYDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Q6</accession>
    <name>U5W0Q6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative molybdopterin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363324.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22790"/>
    <dbReference type="EnsemblBacteria" id="AGZ42828">
        <property type="protein sequence ID" value="AGZ42828"/>
        <property type="gene ID" value="AFR_22790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4619"/>
    <dbReference type="eggNOG" id="COG1319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071554_0_0_11"/>
    <dbReference type="OrthoDB" id="1017413at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="27700" checksum="4EBBACD6E0595B8A" modified="2014-01-22" version="1">MDLHTVVEVVSPAAVGQWRPGDAWLAGGTALFAEPDPGVTRLLDLAAAGWTPITVRDDGLEIAATCTVADLAGFEGAAHFTAFHGLATACCRAFLASFKIWNVATVGGNLCAALPAGPMIALTAALDGECLILGPGGERRMPVARFVTGEGTTALTDGELLRSITLPAAALTGRTALRQGSLFTHGRSAALIAGRLEPGGGLVLTVTGSTVRPFQLRFPDVPAAGLLAAALRDTIADDDYVDDVHGLPAWRQHLTGRFAEEIRAELAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0R7</accession>
    <name>U5W0R7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21610"/>
    <dbReference type="EnsemblBacteria" id="AGZ42592">
        <property type="protein sequence ID" value="AGZ42592"/>
        <property type="gene ID" value="AFR_21610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4381"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080407_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="125"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="237" mass="25998" checksum="D4950D42495EB7F6" modified="2014-01-22" version="1">MDQNLQDGLRPVDLARPHGLSTQAIRNYETAGILPGSERTAHGYRTYTTLHAQALRAFLALLPGHGHTTATAVLQAVNRGATEEALQLIDESHVQLVDDRRTLLAVEDALRDLEPVPPGEGDTFVGPLAHRLGLRPATLRKWERAGVVHPRRDPRTGYRIYSAADVRDALLTHQLRRGGYLLEHIAPVIAQVRSAGGVEPLESMLADWRARLSARSRAMLSGAAALDAYLRHDRMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Y2</accession>
    <name>U5W0Y2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Myo-inositol-1-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363269.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22670"/>
    <dbReference type="EnsemblBacteria" id="AGZ42804">
        <property type="protein sequence ID" value="AGZ42804"/>
        <property type="gene ID" value="AFR_22670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4595"/>
    <dbReference type="eggNOG" id="COG1260">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021486_0_0_11"/>
    <dbReference type="OrthoDB" id="669352at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11510">
        <property type="entry name" value="PTHR11510"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inos-1-P_synth" evidence="2">
        <location>
            <begin position="214"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="378" mass="39362" checksum="56C7D6D6CAAA219C" modified="2014-01-22" version="1">MRTGIWLIGARGSVAVTAVVGATALRARLVEPNGCVTELPQLRSPALPAWSELVFGGHDIVTTPVLKKADALAAAGVLPGRLVSALTADLTAIEHELRPLPAGDTQALVADRIAADLRDFRDRNALDRVVVVNVSTTEPIVTPHPAHADLARLTEALAGPDPVLPPSSLAAYAAFRAVCAFIDFTPSTGARLPALAELARVQGVPFAGNDGKTGETLVKSVLAPMFALRNLRVRSWSGLNLLGGGDGANLADPAANAAKVASKQRVLGDALGYVPEGTSRIDYVAEIGDFKTAWDLVTFEGFLGTGMRMEFTWHGCDSALAAPLVLDLARLTAAAHSAGRSGPLPELGFFFKDPLGDGPSALAEQWATLATFTAGLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W112</accession>
    <name>U5W112_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA polymerase sigma-E factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_22820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22820"/>
    <dbReference type="EnsemblBacteria" id="AGZ42834">
        <property type="protein sequence ID" value="AGZ42834"/>
        <property type="gene ID" value="AFR_22820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4625"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="176" mass="19145" checksum="9ECA04A7135432A3" modified="2014-01-22" version="1">MIPGVQPADEEELVRRIARGDRQAFDELYQRTSPWLAVRLRRRCADEDVVADVLQETYLAVWRAAGSFAGASVSGSAVGWLWTIAAHRLVDAFRRRARQTRTPPPIVSPVRASGAEVTEDLGRALLALPPELRDVLRATVLDGLSVRETALLLGRTGRDGQVTRPAGADRGACPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W174</accession>
    <name>U5W174_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363405.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23120"/>
    <dbReference type="EnsemblBacteria" id="AGZ42894">
        <property type="protein sequence ID" value="AGZ42894"/>
        <property type="gene ID" value="AFR_23120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4685"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_2_2_11"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="17542" checksum="28E84DA199F03B0C" modified="2014-01-22" version="1">MTEARWLDEGEQRAWRSLMVLQEELMEFLDRQLRTRCGLSSADYRVLAHLSETPGGRLRSYELGGLLGWEKSRLSQHLGRMEKRGLVTRERCETDQRGAFAAITPQGADLIRAAAPQHVVDVRAAFIDQLTPAELQTLTVIGDKVRARLGDLSAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1I2</accession>
    <name>U5W1I2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363599.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23705"/>
    <dbReference type="EnsemblBacteria" id="AGZ43009">
        <property type="protein sequence ID" value="AGZ43009"/>
        <property type="gene ID" value="AFR_23705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4803"/>
    <dbReference type="eggNOG" id="COG3435">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136029_0_0_11"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="65"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="18513" checksum="7A05B539B84338C9" modified="2014-01-22" version="1">MSGSGADFSMADFLGAITDTVRPSTARPVVVHAAELERLPADQVHNPTELSIAGRLPTTTFEIFRQTIPAGLSSDMQRHYHETVHYVIAGFGHSEVEDTTESWSTGDFVYTPPWTWHRHYNDSTEEPVEFLTIENSRLLGVLGLVRRQSAGLATVAEARARFKETPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1J7</accession>
    <name>U5W1J7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH_34 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363408.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23135"/>
    <dbReference type="EnsemblBacteria" id="AGZ42897">
        <property type="protein sequence ID" value="AGZ42897"/>
        <property type="gene ID" value="AFR_23135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4688"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142189_0_2_11"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027395">
        <property type="entry name" value="WH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13601">
        <property type="entry name" value="HTH_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_34" evidence="1">
        <location>
            <begin position="21"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="110" mass="12149" checksum="1D5926EF2CB0AEFA" modified="2014-01-22" version="1">MTTEDPAHPVTGLDDVVHQRVRLGILTITHEARRVEFGYLRTQLQLTAGNLSQHLTVLETAGLVDVEKGYEGKRGRTWITLTSAGDKALAEEIGRLKQLIARVETTGTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W252</accession>
    <name>U5W252_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoacyl-ACP reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18725"/>
    <dbReference type="EnsemblBacteria" id="AGZ42021">
        <property type="protein sequence ID" value="AGZ42021"/>
        <property type="gene ID" value="AFR_18725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3802"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_11"/>
    <dbReference type="OrthoDB" id="1582456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="29160" checksum="2E8E6453F335990B" modified="2014-01-22" version="1">MTRLGPYLFAGRTAVVTGAASGIGEQVAHGLAARGSGLVLVDRDAPRLDKVAGSLRADHPDLSVETLVADLADRAAVIRTAEEIRARHPVLGLLVNNAGVALGGRFDQVGLDEFEWVMAVNFTAPLLLIHHLLPALTAERGAHLVNVSSLYGLIAPAGQSAYSASKFALRGLSEALRTELLESGVGVTTVHPGGVRTRIAESARIGAGVPAGEVEGQQRVFAALLTYPPAKAAADILAGVERRRGRVLVAASAKIPDLLARLLPVSHMRVIGRLTAAASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2U8</accession>
    <name>U5W2U8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_19845</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19845"/>
    <dbReference type="EnsemblBacteria" id="AGZ42241">
        <property type="protein sequence ID" value="AGZ42241"/>
        <property type="gene ID" value="AFR_19845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4027"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="46569" checksum="9A325546CAEF45F0" modified="2014-01-22" version="1">MLCAVLFLDGLDVSMVGVALPSIGDELGLSTTSLQWIVNGYVLGYGALLLLGGRTADLLGRRHVFLIALGVFAVASLVGGLVDDGTLLIATRFIKGLAAAFTAPTALSILTTTFHEGPARNRALSVFSVFGASGYSSGLILGGLLTSFGWRWTFLIPVPLALGALAAGIVLIPRDRPAAGGGHDLIGAATLTTGMLLAVYTVVSAPDRGWLDPVTLASAVVAVALLAGFFITEKRVRHPLIRLGILRTGTVVRANLSMIALFGSYLSFQFMMTLYLQNVLGWTPLRMALALLPAGLLVAFGSPFVGRLIDRYGTPRLLISSMTSLLLGYAWFLLTAGNTPHYVVTILPTMLLLGGGFAFGFSSITAQATQGIADSEQGLASGLVQTSGQVGAALVLAVLTALIAGDTTAPATDFSQFHPGVNLVTGVALIGLLLNLVPLLARRRTPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W381</accession>
    <name>U5W381_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26065"/>
    <dbReference type="EnsemblBacteria" id="AGZ43477">
        <property type="protein sequence ID" value="AGZ43477"/>
        <property type="gene ID" value="AFR_26065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5282"/>
    <dbReference type="HOGENOM" id="CLU_1976781_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="13945" checksum="164E4C8854D3008D" modified="2014-01-22" version="1">MVRITVVADDETASDEAMHKLLDELHDFDDIVSVEQATRPGEAPDGAKSAELLAVGTLLLTIVAQPEVLTHLLTYVADWRRRQGRKGRGRIELRTPRGELILTNATDAQADQLIKTFIEEVIDPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3J4</accession>
    <name>U5W3J4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF397 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43577">
        <property type="protein sequence ID" value="AGZ43577"/>
        <property type="gene ID" value="AFR_26575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26575"/>
    <dbReference type="HOGENOM" id="CLU_131550_1_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007278">
        <property type="entry name" value="DUF397"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04149">
        <property type="entry name" value="DUF397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF397" evidence="1">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="63" mass="7066" checksum="755016298BE4706A" modified="2014-01-22" version="1">MRSKMDREVRWISRCADGACVEVALDGEDVLVRDSKRTDGPSLKFSRSEWAAFQDGILAGDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4B4</accession>
    <name>U5W4B4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363319.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22775"/>
    <dbReference type="EnsemblBacteria" id="AGZ42825">
        <property type="protein sequence ID" value="AGZ42825"/>
        <property type="gene ID" value="AFR_22775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4616"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_350184_0_0_11"/>
    <dbReference type="OrthoDB" id="1790198at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="495"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="753" mass="81074" checksum="F41C05BEEE949976" modified="2014-01-22" version="1">MAGWVEHGTADWTELRVHGVSGTPPEAMLEHPTVRRVSGDAASGFYRRVWQARSIAADDRTSVLEAYSWGGLTAGSKLRALWLLLIPFLLANVAFYARPADRPGFRQRLGEAVQRLFALTITATLVLATVNVSMDFAGWQCVRPGGAGSCSSAWAGFLSWPWLDTPGRRIALTALVPIAVVALLWWLANKTWCANEVTPVEAVDADNGQHSPLENRRMWNSARPVRRLRAVHVTAALALVGILALAPFTRDRPTLLSGSLMGVLLATGLLSLVLVCLPSMSDRPDTGTEDDGGASTRFIASLPWLTLGLVAVSLAWMWFAKPVPTGSPGAPRTSLPWLSAAVHIGITVQIVLLIIMVVLLTAVRRHNKGAAWFGYATAILMLFGSALSGAYAAAMVLAVAHLLGKPMPRDRGIDPLVTPMPYFWAAALALVVAAAAVIVGLYGYWSLRRLARGAMLTKVNITYPGNTDPVRARAIAKQWALATADKTLRQVAGRFVALTVVLIVAACIGYAAEQTAVTDTPVLAGMANVGDWLIGLFAAGLLYLGRQTFRNANTRRLVGIIWDLGTFWPRAAHPLAPPCYAERAVPELVNRIDHLRRDGTANVLLSCHSQGTVLGAAVIMQLTYEQSSSVALLTYGAPLDRLYAPFFPAYFGPAQLRRTGSFLVHGTDLPVARWPWRNLYRPSDPIGGPVLGADCVDRPLVDPVFGRAAGDISYPPTLGHSGYTDAPEWAAAVEEVKELRLSQVPYGLLATPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4N3</accession>
    <name>U5W4N3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_29065</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29065"/>
    <dbReference type="EnsemblBacteria" id="AGZ44074">
        <property type="protein sequence ID" value="AGZ44074"/>
        <property type="gene ID" value="AFR_29065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5889"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_135_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="550" mass="59219" checksum="D99FE690D43255CD" modified="2014-01-22" version="1">MNVPGTRLLPHDPSRIGPYELVRRLGSGGMGVVYEARDRDGRRVAVKVVHAELAGDQQFRARFRSEVNRARQVPPFCTAEVLDADPDAPWPWLAVEFVDGPSLAEEVARYGPLSPANLHGLAIGVATALVAIHDAGVIHRDLKPGNVLLAAGAPKVIDFGIAQALDPTSQHTRTGHMVGTVNYMAPERFGAPGAPVTSAADIFAWGCVVAYAGQGRAPFDADSPLGVFGRILHGEPTLDGLSGLLRVLVLRSLDPSPQRRPTARELVDALLGPGPDRPAVPDDVLERQPEMRTEDRRMPTHAVVAPDPVDRPKRRSRALVATVAVLALALAGGLGFVVRTSGWLGPAAQDKQTVVQKEELPALVVPGGSVKLTDPLTAADNWEAYEVENNRTYCRYAGGRLQVRNFQDGLWQCAGPSPDVGGSYAVQVNGRIEKAGSCLVVWFAESAQWRYSLEVCEDAWRLNVEDQGEGEPRVVRQWADSGTDAGQDLRLQMVVQDGTVRVGHDGILIGEHRMGPDFAGVFTLGVASAPATEQGPFHVSYKDIEIRAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4N8</accession>
    <name>U5W4N8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29090"/>
    <dbReference type="EnsemblBacteria" id="AGZ44079">
        <property type="protein sequence ID" value="AGZ44079"/>
        <property type="gene ID" value="AFR_29090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5893"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="PTHR42686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="15"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="35180" checksum="13AB4C57D74E9CCA" modified="2014-01-22" version="1">MAKLLDRGRSPYGVLGLGGAPLGNFATAMSDEEAGRTVARAWERGIRYFDTAPHYGLGLSERRLGAALRDRPRSEFVVSTKVGRLIVPRHPPRTWDDDGFVVPGDLARRWDFSPEGVERSLSESLDRLGLDAVDILFAHDPDQAWDGAAREALASLARLRAAGVVSAIGIGTNSTDGLAPLISEGLVDVIMLANRYSLLDQEALETVLAPAHAAGVAVVAVGIFGTGLLASTTPAPGATYDYRPADTAVRERAGRIASICADHGVELPAAALAFPLLHPAVAAVAVGMRSPSEVDENVRRTGVDIPAGLWRDLVSAGLIPAGSSAPEQPDGADSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5B0</accession>
    <name>U5W5B0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GTP pyrophosphokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29680"/>
    <dbReference type="EnsemblBacteria" id="AGZ44197">
        <property type="protein sequence ID" value="AGZ44197"/>
        <property type="gene ID" value="AFR_29680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6016"/>
    <dbReference type="eggNOG" id="COG0317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1096798_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HDc" evidence="1">
        <location>
            <begin position="38"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="27337" checksum="9706F1D0E9788DEF" modified="2014-01-22" version="1">MGSLVRVLSDTERHQDIPLVWNAYEVAERAHRGQRRVNGDRYISHPIEVATIVARHGGTVPAVCAALLHDVIEDTPFTAAQLRHEFGPDIAALVEALTDQVVREDETAAPELTLVTIADRLHNMRTLRRKPAASRQRASLDTLVFHVPLAQRLDEPALATELADLACATLDALTCDSAETRRLRVLAAMRRADPRLASEVAAALGAGAAVGVPEWALAGGSAGLLALVTAAVFGRDPRAARRLAEILAVWRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5J2</accession>
    <name>U5W5J2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560614.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30085"/>
    <dbReference type="EnsemblBacteria" id="AGZ44277">
        <property type="protein sequence ID" value="AGZ44277"/>
        <property type="gene ID" value="AFR_30085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6094"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_7_0_11"/>
    <dbReference type="OrthoDB" id="1122642at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="26787" checksum="610A0AD09F6FBC2E" modified="2014-01-22" version="1">MDELEWLGIVRHGQSTGNVIAQEAETEGLEAIDIPERDADVPLSETGRDQARAVGQWLADRPAGQRPTIAVVSPYLRTRQTAELALEGTGITAVVDERLRDRELGVLDLLTGHGVRTRLPDEFARRARLGKFYYRPPGGESWADVLLRLRAVLREIRQDHPGGRVLLFGHEATVLLVRYLAESLSEAQLMEIAHATTMANCSISSWRRTGGVLHPEMFNAVAHLHEQGAPPTKQEEVNAEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W619</accession>
    <name>U5W619_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_25660</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364057.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25660"/>
    <dbReference type="EnsemblBacteria" id="AGZ43396">
        <property type="protein sequence ID" value="AGZ43396"/>
        <property type="gene ID" value="AFR_25660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5201"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="26126" checksum="02F86D7CD0EA769E" modified="2014-01-22" version="1">MTTTHAVRADDLVKIYGEGDTAVTAVDRVSVTFPAGEFTAIVGPSGSGKSTLMHLLAGLDTASSGSIRIGGTELAGLKDRELTTLRRDRIGFIFQAFNLLPTLTAEQNIVLPTELAGRRPDRERLAQVVADLGLSDRLGHYPAQLSGGQQQRVACARALMSRPHVVFADEPTGSLDTRTGAGVLSLLRRSVREWGQTIVMVTHDPVAAGYADRVVVLADGRIADDFRAPTPDGVADRLQWLATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6C1</accession>
    <name>U5W6C1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multi-sensor hybrid histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_32450</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561086.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32450"/>
    <dbReference type="EnsemblBacteria" id="AGZ44748">
        <property type="protein sequence ID" value="AGZ44748"/>
        <property type="gene ID" value="AFR_32450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6567"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_51_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="189"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="261"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="329"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="564"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="690" mass="73884" checksum="515D6F68B5655DA5" modified="2014-01-22" version="1">MGVMAYPLRYALADLALALSRASQDATKVRATAAQAAADMLGDGAGIQLLRDDGRYDAITFHHADDELTVPYARLLDREGELPDDDFSTGLAASRRPVVLAGEGSEPLTELPRPRHQAYVDPGGHPPAVHSAVLCPVIVDNTYAGYLVLVRTSPGSIYTEAEVELARDIAGELSLALSSARAMERLRASEERYRRVLETIPEGVLQLDAEGVATYANEPIGVVLGLPRAQLVGVSLRGFLDEHGQRELMRRLAECRAGRYTVGATRLMRADGSQRSVRMSMMPLADEHGQHGGVLCMVTDTTDHIDARGLKRQLDHLRRLDSMGQLIGGISHDFNNLMTVVQGSADMIATTTAEGSPQHQMAKDIMEAVNTGRTLTHQLLAFGRTEGNRPEIIPIPDLLTDVQSLFSRTLGERIGLDLAFGPDVWPVRAERGPLEQALVNLAANARDAMLHGGMLRVAATNEVVEPGQLAEGAPTGRLVHMVIQDTGVGMTDETRRRALEPFFTTRPTAAGLGLATAAGIVQGIGGHIALESQPRMGTTVHLYLPAAEERPASAVDRRTSVIGRVLIVEDQPEVAQLVQRLIAPAGYEITVATDALMAVTQVASGAHPDLLITDVVMPAMTGPELASALRTHHPDLPVLYMSGYTAASLGPQLHLDANSMLVEKPFTRSTLLGAIRSLCGPQPLPGSTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6D4</accession>
    <name>U5W6D4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ClpA-like ATP-dependent Clp protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_31410</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560875.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31410"/>
    <dbReference type="EnsemblBacteria" id="AGZ44542">
        <property type="protein sequence ID" value="AGZ44542"/>
        <property type="gene ID" value="AFR_31410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6357"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090963_2_0_11"/>
    <dbReference type="OrthoDB" id="1708060at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="3">
        <location>
            <begin position="2"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="22419" checksum="525BE23720A1219E" modified="2014-01-22" version="1">MFERFTHAARDAVIRAQEEARELHQKPIGTEHLLLALLADPTGPVANAPALHDVDAAYVRAEVVRRVGPATPYVDERAEADAEDAAALKAIGIDLDAVRRAIEENFGPGALRLPPKATPQRRGLLARFSARTGHTPFSNRSKKILELSLREALRLKHNFIAPEHIMLGIIREGNGLAVQILVEADVDLDRLKEDVTRSLQEQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Q4</accession>
    <name>U5W6Q4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">D-cysteine desulfhydrase</fullName>
            <ecNumber evidence="7">4.4.1.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_32880</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ACC deaminase/D-cysteine desulfhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.15" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32880"/>
    <dbReference type="EnsemblBacteria" id="AGZ44834">
        <property type="protein sequence ID" value="AGZ44834"/>
        <property type="gene ID" value="AFR_32880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6654"/>
    <dbReference type="eggNOG" id="COG2515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048897_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019148">
        <property type="term" value="F:D-cysteine desulfhydrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027278">
        <property type="entry name" value="ACCD_DCysDesulf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780">
        <property type="entry name" value="PTHR43780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006278">
        <property type="entry name" value="ACCD_DCysDesulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="6">
        <location>
            <begin position="5"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006278-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006278-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="29123" checksum="1218A5F261F0544A" modified="2014-01-22" version="1">MTPISLGTWPTPLEAAPRLATALALDELWIKRDDLTGLGGGGNKVRKLQYTCAQALAAGATTLVTTGAPQSNHARLTAAAAARLGLECVLVLAGHPPETARGNLILDRLAGARVVWAGDEATADVAAAQDGFVIPFGGTSAFSALGYVDCARELAVQLPDLDTVVVAVGSGGTMAGLVAVLGPGRVFGVDTGALPDPRSVVAGLLDGMGVPVEPGDLQIDGMHVGAGYATLTDPVRSALELAARTEGLFLDPTYTGRALAGLRANPPDGKVVFLHSGGLPGLFGHPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Y4</accession>
    <name>U5W6Y4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spermidine/putrescine import ATP-binding protein potA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33525</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33525"/>
    <dbReference type="EnsemblBacteria" id="AGZ44963">
        <property type="protein sequence ID" value="AGZ44963"/>
        <property type="gene ID" value="AFR_33525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6785"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="29527" checksum="42352395B166DA26" modified="2014-01-22" version="1">MTAIEVSGLRKAYGGRPAVDGISLSIEHGEVFALLGPNGAGKTTTVEILEGHRRRDAGAVRVLDADPGNAGRAWRAKIGIVLQSATDAADLTVTEIVRHVAGFYPRSRPPAEVVSLVGLTDKSRARVRSLSGGQRRRLDVALGIVGRPEVVFLDEPTTGFDPEARRQFWDLVRALAAEGTTILLTTHYLDEAEALADRLAVIADGVIVAEGTPATLGGRAEAGVLVTWVDRNGRHEQRTDDPAAVIAELTAAGTDLGSLTVARPSLEDTYLQMIGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Z4</accession>
    <name>U5W6Z4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561310.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33575"/>
    <dbReference type="EnsemblBacteria" id="AGZ44973">
        <property type="protein sequence ID" value="AGZ44973"/>
        <property type="gene ID" value="AFR_33575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6795"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_1_0_11"/>
    <dbReference type="OrthoDB" id="1925754at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="24"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="166" mass="17598" checksum="65B67380058DCF6B" modified="2014-01-22" version="1">MPHINLPNLPGIVGLLVQFPQTAGPLNGLADALLRGPSPLTPAERETIAAYVSSRNKCTFCAETHGAVARHLGQPEEHSPKMQALLALADKVRVDGRSVTPADIDQARAEGADDKTIHDTVLIAAAFSMFNRYVDGLATLTPENPAAYAQHAKNLAQGGYLTTPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Z5</accession>
    <name>U5W6Z5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbon-monoxide dehydrogenase, large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560039.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27200"/>
    <dbReference type="EnsemblBacteria" id="AGZ43701">
        <property type="protein sequence ID" value="AGZ43701"/>
        <property type="gene ID" value="AFR_27200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5512"/>
    <dbReference type="eggNOG" id="COG1529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_11"/>
    <dbReference type="OrthoDB" id="117507at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012780">
        <property type="entry name" value="CO_Mo_DH_lsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="PTHR11908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="Ald_Xan_dh_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="SSF54665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="SSF56003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02416">
        <property type="entry name" value="CO_dehy_Mo_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ald_Xan_dh_C" evidence="2">
        <location>
            <begin position="36"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="798" mass="85463" checksum="EED2456A03B67CF1" modified="2014-01-22" version="1">MTTVQDAGKLTTFEDNDQKPVGHGRMLRKEDPRFVRGRGRYTDDVQLPGMLHLAILRSPFAHAKIVSIDTSAAAALPKVKAVVTGADLAAQGLAWMPTLSNDVQAVLATDKVRFQGQEVAFVVAEDRYAARDALELIDVEYEVLDPVIDVRKALAPEAPVIRDDLDGKTNNHCFDWETGDKAATEAVFAAADVVVSQDIVYPRVHPAPMETCGAVADFDPVEGKLKLWSTTQAPHAHRTLYAIVAGIPEHKIQVISPDIGGGFGNKVPIYPGYVCAIVASIVTGKPVKWMEDRSENLISTGFARDYIMRGEIAATRDGKILGIRTDVLADHGAFNGTAAPVKYPAGFFGVFTGSYDIEAAYCSMTAVYTNKAPGGVAYACSFRITEAVYLVERIVDCLAAELGMDPAELRLKNFIKPEQFPYTTKTGWVYDSGDYEPTMRLAMDMAGYADLRKEQAEKRERGELMGIGIAFFTEAVGAGPRKDMDILGLGMADGCELRVHPTGKAVVRLSVQSQGQGHETTFAQIVAEEIGIPPADIDVVHGDTDNTPFGLGTYGSRSTPVSGAAAALVARKVRDKARLIASAMLEVSVADLEWEKGAFQVKGDPGKSVTIQDIAMRAHGAGDLPEGVEGGLEAQICYNPSNLTYPHGAYICVVDIDPGTAVVKVRRFIAVDDCGNRINPMIIEGQVHGGLTDGVGMALMEMISFDEDGNCLGASLMDYLIPTALEVPDWETGFTVTPSPHHPIGAKGVGESATVGSPPAIVNAVVDALAPFGIRHADMPLTPSRVWDAMRGAPQPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Z8</accession>
    <name>U5W6Z8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561315.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33600"/>
    <dbReference type="EnsemblBacteria" id="AGZ44978">
        <property type="protein sequence ID" value="AGZ44978"/>
        <property type="gene ID" value="AFR_33600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6800"/>
    <dbReference type="eggNOG" id="COG2114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2552077_0_0_11"/>
    <dbReference type="OrthoDB" id="1989751at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025336">
        <property type="entry name" value="DUF4242"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042557">
        <property type="entry name" value="SCO4226"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14026">
        <property type="entry name" value="DUF4242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="82" mass="9224" checksum="443D779A43CEC4B7" modified="2014-01-22" version="1">MAKFMDVHSGFFGVTHEELDAAHCADLKIEAEEGVHFERAWLDPELGKVFCLSTGPSKEAILRIHERAGHPTTEIYELTEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7J9</accession>
    <name>U5W7J9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein gmr</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34155</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34155"/>
    <dbReference type="EnsemblBacteria" id="AGZ45089">
        <property type="protein sequence ID" value="AGZ45089"/>
        <property type="gene ID" value="AFR_34155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6911"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="361"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="500"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="762" mass="81916" checksum="29A4B4A84E0F13C6" modified="2014-01-22" version="1">MPLALWVAVAVASVLVVLAPLVSTLGGLATLMLGSAILVMASIGLGVRLHRPRYPRAWWLILVSVALALVGNVIWSTVLPSSITSDAAAINVIYGITYPILCAGVALLPNHRRPGAAMIGMTESAIIACGVAVVWWMLFVDPRLIDTGGIETQAYLLVEPFLDLVLFGLGVRLLLFSDSHAVCYWLVAASCAARLAADTAYLTYGSSAGFPTATSVIGWVVCNGLLAVAALHPSMARTVPLPANEITGRYSATRIYIGTVVATPVLTGIFLVREGLKDTLSSLDVVVPVTATAVTSALVVQRMRQLHRVAARRATELEQALNTGEVLQQELLFRSSHDALTGLPSRSVLHQRLTAVLAADPRGALLLLDLDKFKDVNDRFGHTVGDDLLVEVSALLREETGGSLVARLDSDEFAVLLTDAGPDEVRATAERVLAALRRPVRVQGHEMFATASAGLRDLADDLLSADVVRDAYVALHAAKEAGRNQLALFDRELRAKRLETARTVERLRGAIDRDELLVYYQPLIRLADERITGVEALIRWQPAGERMIPPDRFIGAAEDSGLIVPIGAWVLQESCRVAAGWHRRHGTVVSVNVSPRQLREPDFGAMVLDALRSSALPPEALILEITEGVLVNSGVATEQAISHLSMVRSHGVRVAIDDFGTGYSSLAYLRDLPIDHIKIDRSFMPAPGVTDPASRNLVKAIIDLAAGLELSVIAEGVETAEQVTLLRELGCERAQGFYFSRPVPEADADTLIAEDHHGTVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7L6</accession>
    <name>U5W7L6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561520.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34635"/>
    <dbReference type="EnsemblBacteria" id="AGZ45183">
        <property type="protein sequence ID" value="AGZ45183"/>
        <property type="gene ID" value="AFR_34635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7007"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_11"/>
    <dbReference type="OrthoDB" id="1209997at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="28396" checksum="F662AD420C64952A" modified="2014-01-22" version="1">MDLQLTDKVAVITGGTVGIGLAVARGLAAEGAHLALCARDTDRLEAVVQALRTEFPVRVLGVTADVGGLDDITNLARAVTDEFGGADILINNAGTGSEETILEASDERWQAYWDLHVMAAVRLARVFAPQMKARGGGVILHNASICATQPLGYEPIYNVTKAALVMFSKCLANELIGDNIRVNTVNPGLVQTPDWEKTARALTEGTDRSWEDHLQQIADDYAPIGRFADPAEIANLFVFLCSPRASYMVGSSYYIDGGWLRTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7R0</accession>
    <name>U5W7R0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560367.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28845"/>
    <dbReference type="EnsemblBacteria" id="AGZ44030">
        <property type="protein sequence ID" value="AGZ44030"/>
        <property type="gene ID" value="AFR_28845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5847"/>
    <dbReference type="eggNOG" id="COG1075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_2_0_11"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="5"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="24166" checksum="1B8ECC4BBDAF45DB" modified="2014-01-22" version="1">MKPTIVLVHGAFAESASWSGVIQRLHTDGYPVVAAPNPLRSLSGDAAVVSSLLATIEGPVVLVGHSYGGAVISNAAVGHENVKALVFVAAFAPEAGESIGELSGKFPGSTLGETVHEVPLADGTVDLYIEQAKFHQQFAADVPAEQAVIDAAVQRPLNTTALNEGSGEPAWRTVPSWFVYPELDRNIPLEAHRFMAERASSHLGVELPGASHAIPASEPAAVAGVILAAATSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W813</accession>
    <name>U5W813_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TetR_C_36 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44110">
        <property type="protein sequence ID" value="AGZ44110"/>
        <property type="gene ID" value="AFR_29245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5926"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097446_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041485">
        <property type="entry name" value="TetR_C_36"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18598">
        <property type="entry name" value="TetR_C_36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TetR_C_36" evidence="1">
        <location>
            <begin position="71"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="20039" checksum="2CF8D9C0869DA22E" modified="2014-01-22" version="1">MISHETVVRGAIRYFLEHGTVDVDELAPILAVSRATLYRVAGSRELLLGHVLWALCEIRLDAARRARTVDGIEGVLEVTRHFGAGLADAKGLITFVTTEPELAGRVLSSPTGVVHPRVVAAQKEIFLEAGCGAGPGPAVDLDRLAYLYTRMVEVAAYSELQYPGRPDRGPVEQALRALLTEAWPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W838</accession>
    <name>U5W838_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD_binding_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560472.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29370"/>
    <dbReference type="EnsemblBacteria" id="AGZ44135">
        <property type="protein sequence ID" value="AGZ44135"/>
        <property type="gene ID" value="AFR_29370"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29370"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5952"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_20_3_11"/>
    <dbReference type="OrthoDB" id="867226at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="5"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="499" mass="54050" checksum="ACF1D31078C3BB05" modified="2014-01-22" version="1">MTDRIDVLVAGAGPAGLALACDLQRRGVKLRVIDAADRSFEGSRAKGIQPRTQEVLDDLGILADVQAHSALYPLLGAHVGPLTLPWMMIKHRTPSEDVPHPNTLLVAQYNTDAAIKRRLEELGGTVEYRTRLTGYEQDADGVVATVETPAGTEQISARFLVGADGGGSVVRRTAGIDFIGDTDDSDRMIVADVTLTGLPRNRWHIWPRGNGRFMALCPLPDGEKFQLMLKLRPDEKADLNQESVNRLVEQFVGNTKLHVVQVHWASVWRPNIRLAQRYRSGHVFLVGDAAHVHPPTGGQGLNTGVQDAYNLGWKLAQALAGAPDFLLDTYEAERQPIAARVLGLSTDIYARMSSRPLAAAKRGDEERQLALNYRGGPLAADSGEDSPKVIAGDRAPNARHDAGQLFDDFRGAHFTLIAVGEPAVAALKQISWPGTGAELKTVEIPEKDAAGVRRIYGITGPAHILVRPDGYVAYVTQGDWTGALSKYTELMLPLTSSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8A5</accession>
    <name>U5W8A5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="5">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="5">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">truB</name>
        <name evidence="9" type="ORF">AFR_35935</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561779.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35935"/>
    <dbReference type="EnsemblBacteria" id="AGZ45443">
        <property type="protein sequence ID" value="AGZ45443"/>
        <property type="gene ID" value="AFR_35935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7274"/>
    <dbReference type="eggNOG" id="COG0130">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_0_0_11"/>
    <dbReference type="OrthoDB" id="1166299at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02573">
        <property type="entry name" value="PseudoU_synth_EcTruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015225">
        <property type="entry name" value="tRNA_psdUridine_synth_fam2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="PTHR13767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09142">
        <property type="entry name" value="TruB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00431">
        <property type="entry name" value="TruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TruB_N" evidence="6">
        <location>
            <begin position="25"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="TruB_C_2" evidence="8">
        <location>
            <begin position="178"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="TruB_C" evidence="7">
        <location>
            <begin position="236"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30719" checksum="C4BAD7EB388E78A8" modified="2014-01-22" version="1">MNVDGLIVVDKPAGMTSHDVVARVRRLAKTRRVGHGGTLDPMATGVLIIGVNRATRLLTYVIGSEKSYAGTIRLGQSTVTDDAEGDVTVTAATDGVTDEGIRAGLAAQTGEIDQVPSAVSAIKVNGQRAYKRVRDGEVVELAARRVTVSRLDVLDIRREQGVVDVDVDVTCSSGTYIRAIARDLGATLGVGGHLTALRRTQVGGLSLAEAATLPELEERAPDVISLSMADAARQAFPQRAADAEETRVLRHGGPLEPVGIDGPYAVFDPEGDLLAIVSERGGKARAEIVLSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8C5</accession>
    <name>U5W8C5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560567.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29845"/>
    <dbReference type="EnsemblBacteria" id="AGZ44230">
        <property type="protein sequence ID" value="AGZ44230"/>
        <property type="gene ID" value="AFR_29845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6045"/>
    <dbReference type="eggNOG" id="ENOG502ZDUZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1383509_0_0_11"/>
    <dbReference type="OrthoDB" id="1475689at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="18580" checksum="0C57091A2EA0D785" modified="2014-01-22" version="1">MGIDYGYDLYLPTHAVGKALLAVAAIAREDIGSVEVVAPGGERITLPFRGKPANDVDHWSLDACLYFPLGDEAIRAWAEVERREGRQDAQERIWVGSIYLYFWRDSGLRPGYSRLDFTAATTSMSRLFEQSASIRGTFTELAKSVGAARLVLDREGDGDEVCWSGDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8D2</accession>
    <name>U5W8D2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative chromosome partitioning protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560572.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29870"/>
    <dbReference type="EnsemblBacteria" id="AGZ44235">
        <property type="protein sequence ID" value="AGZ44235"/>
        <property type="gene ID" value="AFR_29870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6050"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_1_0_11"/>
    <dbReference type="OrthoDB" id="729012at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="55"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="310" mass="33725" checksum="917311887F20AAC3" modified="2014-01-22" version="1">MSGNHDRAEAWTSALRDQQSSLDLGADLGPADPTAYTMRRPIPEPMPTDRHGPARIIALANQKGGVGKTTTTINLGAALAEYGRKVLLVDFDPQGACSVGLGVNPHNLDLSIYNLLMQDDVTAEDVIIKTDVAGLHLLPANIDLSAAEIQLVNEVAREMALARVLRTVRKEYDFILIDCQPSLGLLAINALTIAHGVLIPLECEFFSLRGVALLLDTIDKVRERLNFDLELEGILATMYDSRTTHCRQVLQRVVEAFGDKVYQTVITKTVKFPESTVAGAPITSLDPASSGARNYRQLAREVIAAKDERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8D9</accession>
    <name>U5W8D9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative channel-forming protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560582.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29920"/>
    <dbReference type="EnsemblBacteria" id="AGZ44245">
        <property type="protein sequence ID" value="AGZ44245"/>
        <property type="gene ID" value="AFR_29920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6060"/>
    <dbReference type="eggNOG" id="ENOG5032TBA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072020_0_1_11"/>
    <dbReference type="OrthoDB" id="1520742at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021522">
        <property type="entry name" value="MctB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11382">
        <property type="entry name" value="MctB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666198" evidence="2">
        <location>
            <begin position="29"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="32309" checksum="2474E9C0BA73377A" modified="2014-01-22" version="1">MINFRYHVVSLTAVFLALAIGLVVGTAALNGPVADTLKDRVDALSKDNSNYREQANQYREELNRTQDFATEVSPALLNGKLAERKILIVVMPSGQDYAEGVTAMLKVAGAKITGTVTVQDKFFDPANAYELLDLADKASQPTVPAEGLPLNSDGVETSSALLSIALLQQTTPVTPSDLTAVLAAYTEPSYLSVSDDIVPGAEATVVVSGLPPVDRDAPKKNQNALTFTTQFANNRPLVVGGNGIGDGNLVAGVRADPNLVKKISTVDNVSTEQGRLATAMTVIERVVAGKVGQYGLSAGATSQIPKAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8U7</accession>
    <name>U5W8U7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose reductase</fullName>
            <ecNumber evidence="2">1.1.1.133</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_30800</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57510"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="1.1.1.133"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the dTDP-4-dehydrorhamnose reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.133" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560755.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30800"/>
    <dbReference type="EnsemblBacteria" id="AGZ44420">
        <property type="protein sequence ID" value="AGZ44420"/>
        <property type="gene ID" value="AFR_30800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6235"/>
    <dbReference type="eggNOG" id="COG1091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045518_2_1_11"/>
    <dbReference type="OrthoDB" id="1076083at2"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="PTHR10491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD_sub_bind" evidence="3">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="27104" checksum="6DEA3EAFD36B78B9" modified="2014-01-22" version="1">MNALVVGASGHLGGEVARLASAAGTSTTGSGGWSRLDITDRRAVLDLFASVRPQYVINTAYRASSWTICADGAANVALAAVAVGARLVHVSSDAVHGGRPTPYLDDDAPTPVYPYGAAKAAAETAVAAIDPAAVLVRTSLIIGDERSAQVRLALDLTTGRKQGALFTDEVRCPIDATDLAAAIVELASSDYAGLINVAGPEVLTRAELGRLIAVRHGLDPATVPTCTIAESGMGPRPADIRLDSTRAVGLLETRIRGAAEALSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W945</accession>
    <name>U5W945_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="7">Glu-ADT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">gatA</name>
        <name evidence="9" type="ORF">AFR_36975</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561985.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36975"/>
    <dbReference type="EnsemblBacteria" id="AGZ45649">
        <property type="protein sequence ID" value="AGZ45649"/>
        <property type="gene ID" value="AFR_36975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7480"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_11"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="497" mass="51837" checksum="B29CDD0ED24ECCCF" modified="2014-01-22" version="1">MSDVTRMTAAQIAGLISSGEASAVEVATAHLARIDAVDERVHAFLHVDREGALEAARKVDEQRSKGEALGPLAGVPVAVKDVIATKGVPTTAASKILEGWKPPYDATIVERLRGAGTVMLGKTNMDEFAMGSSTEYSAYGATNNPWDLGRIPGGSGGGSAASLAAYEAPLAIGTDTGGSIRQPGAVTGTVGAKPTYGGTSRYGLIAFSSSLDTPGPCARTVEDAALLHSVIAGHDVRDSTSIPQAVPDVVAAARLGATGDLTGVKLGIVKEFAGEGAEPGVMAAFRESVEALVKLGAEVVEVSCPHFEFALPAYYLIAPSEASSNLARFDGVRYGLRVGDDGNRSLEEVMSLTREAGFGPEVKRRIILGTYALSSGYYDAYYGQAQKVRTLITRDFTTAFEQVDVLISPTTPFVAFPFGSRTSDPYQMYLADLYTIPTNLYGGPAISVPCGLSEGLPVGFQVMAPTMADDRMYRVAAALESAVGTFTPPELSLESAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9A6</accession>
    <name>U5W9A6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP synthase subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP synthase F(1) sector subunit delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">F-type ATPase subunit delta</fullName>
            <shortName evidence="6">F-ATPase subunit delta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atpH</name>
        <name evidence="7" type="ORF">AFR_37350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="6">This protein is part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ATPase delta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37350"/>
    <dbReference type="EnsemblBacteria" id="AGZ45724">
        <property type="protein sequence ID" value="AGZ45724"/>
        <property type="gene ID" value="AFR_37350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7556"/>
    <dbReference type="eggNOG" id="COG0712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088880_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="PTHR11910"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0139">CF(1)</keyword>
    <keyword evidence="2 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="3 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="272" mass="28789" checksum="44B7E424AFE6D637" modified="2014-01-22" version="1">MASVNRESYAAAAEKLTAATQQATAAQLATTADEFLSVARLLRGEPRLRRALTDPSRSGGDRAELVRSILAGKLGDTTVDTLATLVSGRFSRPSELLDASERLGVDTVLSSADKAGTLAEVEDELFRFGQIVSGNAELAVTLSDPSAPAEQRVKLAQDLLKGKAQAATIQLIAVALEGFGGRSFESSLSRLVELTAAKRDREVAYVTVAKLLGEAEEQALAAKLSDIYGRQVSLKVDVDPSIVGGVSVRVGSDLYDGTILRRLNEARQAFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9E3</accession>
    <name>U5W9E3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative signaling protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_31650</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31650"/>
    <dbReference type="EnsemblBacteria" id="AGZ44590">
        <property type="protein sequence ID" value="AGZ44590"/>
        <property type="gene ID" value="AFR_31650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6406"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="390"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="530"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="789" mass="85225" checksum="72C47DF726983738" modified="2014-01-22" version="1">MRQEDRATMTIGDERHVERLRPTPMLITAVAGLAAAIVLFAVNVTEQRTQVVWGWMPAMAGTAIAGWACWRTAFTPGLDKVTARVWRSLAIVCVLIVLGTTGDARLTVANPARLDQEHDLPTAICYALAVVVLLWALLRLPMHKREHRDRAMVRFVLDAATVSITIGIFSWFLVNQVHDTWNSNGPAIVPMIMLMALGLIASLAFVKVAISGFGGVDHIALRLLAAAAAVGAGGGGLFPLLLDVRPGLSGSQMFIPATLLFVALAADRQRRAAGVPQPERRRRSYSIVPYVAVAATDGLLLTIGHGAGREIFTVALGAVLLTALVVFRQVNALRENGRLLQRVDANLVQLNAYQDQLTHRANHDDLTDLANRTLFEQRTREALVAVDGGDTLSLALIDLDDFKAINDRLGHAVGDALLVVVAERLRAAVRDGDVVARLGGDEFGLLLPGLHSEEATEVLDRIAESLTRPVHALGYDLLVKASIGLAEVWPDASPQELLRRADLAMYAAKERGKGRHTVYDARLEQDQAADAQLGAELRRALDNGDFTLVYQPIVSLPDGKWTSLETLIRWKHPDRGFIGPDIFIPIAERTGLIVPLGDWILRTALAQAAEWQDRFGAGAPGEIGVNVSARQLREPGFAADVRAALADTGFDPEKLVVEVTETAVFDGGIALDTLQNLVTLGVRIALDDFGTGHSSLGLLRTCPADILKVDKSFVDGIGGRSEEAVIATAMIQITNGLHLQAIAEGVETAEQADTLFRLGYRYAQGYHFSRPLSPEQVHAQLEAASVLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9F5</accession>
    <name>U5W9F5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561988.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45652">
        <property type="protein sequence ID" value="AGZ45652"/>
        <property type="gene ID" value="AFR_36990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7483"/>
    <dbReference type="HOGENOM" id="CLU_1709344_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16529" checksum="5E8C8AAE9D9B1ECB" modified="2014-01-22" version="1">MLIHVPVGRPPRRRRLFTNRAFTLEAISFRAVDGRTVTEAIGLRPVTRKAIDLGPVTRSAFIVEVVGVRAVTRRAINRPTVTCRAVNCQAVTCSAARRRPVTRGAFILEAVGLKRATRKAVGGRARVQGSAIHPSAARRPGARRPGAHTRVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9G5</accession>
    <name>U5W9G5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetracycline repressor protein class H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560948.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31775"/>
    <dbReference type="EnsemblBacteria" id="AGZ44615">
        <property type="protein sequence ID" value="AGZ44615"/>
        <property type="gene ID" value="AFR_31775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6432"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_0_1_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="24509" checksum="A4195AD8A9A3FDB4" modified="2014-01-22" version="1">MSVEFLWGDRARPTRGPKPTMSLESIADAAIVLADAEGLAALSMQRVAADLGFTKMSLYRYLPGKAELVALMVERAIGEPPELPASGWREGLVAWSELLFAAYLRHPWTVEATVGRRHLGPNELGWMECALARFGADSQLTGAERLDAIAVLAGHARMLAQQSAAQLGEAEMTRAMGEVMQVHGDRFPHLTAAMLQTAAEGGQNQAFTFGLHRILDGLETLLSRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9H2</accession>
    <name>U5W9H2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uxu operon regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_38045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38045"/>
    <dbReference type="EnsemblBacteria" id="AGZ45863">
        <property type="protein sequence ID" value="AGZ45863"/>
        <property type="gene ID" value="AFR_38045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7696"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24987" checksum="446E3B6DAB6D155C" modified="2014-01-22" version="1">MAGSTVRPPAYQLLADELREEITSGRLQPGSRLPPEPELCVRSGVSRSTVREALRLLASQHLIVTTRGVTGGSFVAHPDAEQLSEALSTGLSLLSNSAEVGMADLLELRRALEIPAAGLAAERRTDANLVEMRGAMIDPDLDELSTMLAAHAAFHSAVASATGNPLYELVTRPLYHVTYGEEVVENLPHDYWLKIDADHRELLSCVRSRDSAAAMAVGRRHLDYIATANAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9H6</accession>
    <name>U5W9H6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31480</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560889.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31480"/>
    <dbReference type="EnsemblBacteria" id="AGZ44556">
        <property type="protein sequence ID" value="AGZ44556"/>
        <property type="gene ID" value="AFR_31480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6372"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_0_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="6"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="147"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23305" checksum="C9328B6CC1686FFC" modified="2014-01-22" version="1">MPEVTRVVLAEDGVLLREGLVGVLSRFGFAVVAAVGDGDALLEAVAEHRPDLVITDIRMPPDHTDEGLRAAVTLRREQPGLAVVVLSQYVQEEYASALLDTGDGLRVGYLLKDRVVDVEDFVAALRTVLGGGTVVDPEVVRRLLRRPRDPLAALSAREREVLGLLAEGHSNSAIAARLFVTEAAVGKHVGNILAKLDLPPAEDTNRRVLAVLAFLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA81</accession>
    <name>U5WA81_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative glutamate--cysteine ligase 2</fullName>
            <ecNumber evidence="2">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Gamma-glutamylcysteine synthetase 2</fullName>
            <shortName evidence="2">GCS 2</shortName>
            <shortName evidence="2">Gamma-GCS 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33185"/>
    <dbReference type="EnsemblBacteria" id="AGZ44895">
        <property type="protein sequence ID" value="AGZ44895"/>
        <property type="gene ID" value="AFR_33185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6716"/>
    <dbReference type="eggNOG" id="COG2170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017048_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042398">
        <property type="term" value="P:cellular modified amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01609">
        <property type="entry name" value="Glu_cys_ligase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025841">
        <property type="entry name" value="CP_ATPgrasp_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011793">
        <property type="entry name" value="YbdK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14403">
        <property type="entry name" value="CP_ATPgrasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02050">
        <property type="entry name" value="gshA_cyan_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="864" mass="92449" checksum="B9E2302C9B045F16" modified="2014-01-22" version="1">MTESLKVSEDDGAKEASDTIGTDLTLGVEEELHVIDLKTRELVPRAPEVLDQLDAANYSAELHRSVVETNTPVAATLDDLRDGIVSLRRKAIGVAESLGLGLVAAGTVPLVDLDSLPVTPTSRYRRMVDEYQMLAREQLICGAQVHVGIPDRDEAVSIAQRVAPALPVLLGISASSPYWMGEDSGYASVRSLVWLRWPTAGDSGPVTSAADHDALVADLIASGTISDPKMVYFDVRPSAHLPTVELRVTDSSPDADTVVLLAGLFRGLVLRARQDYRAGRPIVPTRPPIHRAAMWRAARSGLEGDLLDLPRSPVPVPAAVAVERLVGDLRPQLEELGDWEQVFDLSVQALSRGSSAARQRRAMARRGRISDVVDLVVADTRGGATGIGPDGQTVAAGLTPYAATGDEAFPGGDVVPAYQGIVNVLSALGPAGLRRREDAKDDEQRARGITFSVAGEAATRLFPFDLVPRIVPGSDWRELQTGLIQRVRALDAFVNDVYGDRAVVKDGVVPDWVIDGSPELRPSGALVSRPGIRAQVAGVDLVRDGEGNWCVLEDNLRVPSGIGYAMQNRRLTESVLPELPRPDELLSIEDTPKLLFKALRDAGGPAAGDDPAVVVLSQGPDDSAWFEHRMLAEEMGVPLVRSTDLLVDDGIVHRIVSGRKHRVDVIYLRMGEDSLVHSPGADGMPLGPSLVTALHADTIVLANALGNGIGDDKAVYAYVPKLIEYYLGEKPQLADVPTYLCGIPEQKAEVLGRLEELVCKPVDGYGGDRIVIGPHASPSDLAAVRRQINAAPHRWVAQEVVDLSTHPVFDGHQLAPRHVDLRAFVFTGTTSVVAPAALTRVAPAGSMIVNSSRGGGSKDTWLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAY2</accession>
    <name>U5WAY2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative two-component system sensor kinase PhoR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_40275</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40275"/>
    <dbReference type="EnsemblBacteria" id="AGZ46309">
        <property type="protein sequence ID" value="AGZ46309"/>
        <property type="gene ID" value="AFR_40275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8151"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="197"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="447" mass="46988" checksum="349346E35DA6FD45" modified="2014-01-22" version="1">MDPVFGTTLVLVALAVGVLAGSALSRYRRTAAPAEEPGGAQAAKAGKAKAGKAEVRPIEGGRKIDTDDDRMGKHGLKGLGRKSLDSLRVGVVVLDAEDEPVLVNPAARAMGLLRSGGAPGTIAAHPILRTLAGQVRRTGVRREVELDLPRGRAGGAQAPLGLHLRAVALNSTHVAVEAADVTESHRLARVRRDFVANVSHELKTPIGALQLLAEALLDATELPAAEAAEQSEDLIAARRFAERIHHESARMGRLVNELLELTRLQGAEPLPTPEPVALDWVIAEVVDRTRTTASAKGIEVVYTGPKGSTVYGSDSQVATAVTNLVENAIAYSGEDTQVVLTMREADDWIEIDVTDQGIGIAPHDVDRIFERFYRADQARSRSTGGTGLGLAIVKHIATNHGGRVDVTSTLGGGSTFTLRLPARPPESPMPLPAAIEIESGAAGLPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB62</accession>
    <name>U5WB62_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative 5'-nucleotidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562716.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40650"/>
    <dbReference type="EnsemblBacteria" id="AGZ46384">
        <property type="protein sequence ID" value="AGZ46384"/>
        <property type="gene ID" value="AFR_40650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8227"/>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_808056_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665747" evidence="1">
        <location>
            <begin position="35"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="36340" checksum="180165771D5999B5" modified="2014-01-22" version="1">MDSVRPRGLAALLTLALGAGTVAGAGLLGSPAWAAGPLSGTYSLDSTSIWTAQQVTLTQTALEDETPDTAVERTVDWGDGTVQTLSADAVKAVHKYPANGSYAVKVSLKDGDATADGTISNATSTVTVATAGGTYKFNPTWNWTWAGGGYNAKYELSGVPANATRVWVNWGDGETSLLTRSTTSTYHYYGFGQYTATVTLENAQGKTVPKAAGWYSTVADESSPSATLTVPSSPSKASSWSTVKGTAKDLQIGIDAVGVQLWRWTSSSDYYYNFSTKKWVKFNPDSTTVPNAALSWRPVDSKGNWSVGVSGMAKGYTIEVDYAAVDRAGNYSAWKYKVQKLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBZ2</accession>
    <name>U5WBZ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_36295</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561851.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36295"/>
    <dbReference type="EnsemblBacteria" id="AGZ45515">
        <property type="protein sequence ID" value="AGZ45515"/>
        <property type="gene ID" value="AFR_36295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7345"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100874_3_0_11"/>
    <dbReference type="OrthoDB" id="1345242at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="17011" checksum="CD9F2B48584F0C81" modified="2014-01-22" version="1">MTFIDRRAARVLLIDAAERALLLRGSDPARPGERWWFTPGGGLAEGETPVQGAARELHEETGLKVEPSALGEPVFHEITEFSYNNRQYRQEQDFFLFRVPDWQVDTAGFDDDERRTITEHRWWSAPELDATTEQIFPLGLADLLRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCA7</accession>
    <name>U5WCA7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mannosyl-3-phosphoglycerate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36880"/>
    <dbReference type="EnsemblBacteria" id="AGZ45630">
        <property type="protein sequence ID" value="AGZ45630"/>
        <property type="gene ID" value="AFR_36880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7461"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028916_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050504">
        <property type="term" value="F:mannosyl-3-phosphoglycerate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051479">
        <property type="term" value="P:mannosylglycerate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012812">
        <property type="entry name" value="Osmo_MPG_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09488">
        <property type="entry name" value="Osmo_MPGsynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02460">
        <property type="entry name" value="osmo_MPGsynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="346" mass="37882" checksum="F7734462FE879ED5" modified="2014-01-22" version="1">MLSGIPHDCLIVLVSNSDKLPVDRYEIEAQTVEQFCRLAGRSAISVHQKDPGLAAAFKAAGMEELIGEDGLVRSGKGEAMLIGMALAAMTGRRYLGYVDADNYVPGAVHEYCKAYAAGLHLADSPYAMVRISWHSKPKLRDGRLFFSRRGRSSEITNEWLNRFLAEYSGFGTEVIATGNAGEHAMSLDLGLKLRLAGGFAVEPYEYLDLFEQFGGVLESTSPDVMSSSVPVLQIETRNPHFHDNKGEDHVQGMRMQALNVVYHSPVTLPSVRQAIIEFMVAQGALEVGQEPPRERVYPPVGSLDLELLYDGLDAEALTFRQFDGLASAGYAPAPPIERNLIPRPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCC6</accession>
    <name>U5WCC6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatC</name>
        <name evidence="3" type="ORF">AFR_36980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561986.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36980"/>
    <dbReference type="EnsemblBacteria" id="AGZ45650">
        <property type="protein sequence ID" value="AGZ45650"/>
        <property type="gene ID" value="AFR_36980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7481"/>
    <dbReference type="eggNOG" id="COG0721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105899_1_0_11"/>
    <dbReference type="OrthoDB" id="1780195at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="99" mass="10849" checksum="D5D79ACC0A6FD6F0" modified="2014-01-22" version="1">MAAISREEVAHLARLSRLAVTEQELDRFAGQLDVILQSVARIGDVAAEDIPPTSHSVPLTNIYRDDVVQPSLTQEQALSGAPDSAEGRFRVPRILDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCV6</accession>
    <name>U5WCV6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclic nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562175.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37930"/>
    <dbReference type="EnsemblBacteria" id="AGZ45840">
        <property type="protein sequence ID" value="AGZ45840"/>
        <property type="gene ID" value="AFR_37930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7671"/>
    <dbReference type="eggNOG" id="COG1994">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_290967_0_0_11"/>
    <dbReference type="OrthoDB" id="252759at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="915"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="953"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="979"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1043"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1050" mass="110075" checksum="519B1769CDFC9ED9" modified="2014-01-22" version="1">MTYVETRMSVWEALAGRAPGEPLGPADPGLWGAVVDRLNPARARPVLRAGIEAVELISVRGSAYVMLRSPDDGGRACYLRLTPDEWQLALMMDGTHTVARLVAEFARLAGRLAPDQVRRVVADLAGNRMLDELPVDAFRPLQELERQPLPKRVGSGVLAAARGRRMLVFDVDGVVSALYRAGGRLLFTRVAAIVLGVFAVAGLGLFVATWWRGSQSVFLTNGSYLLGAIVLLLLNLVALASHELGHALATKHAGREVPAAGVLVYFGIPSVFVDTTDVWMAGRRARMLVTAAGPATGLVLAGSMQLIGLAVPALGPLAFKLAFAWYLNVLFNLNPLLALDGYYLLMDWLEIPNLRGRGLSWVTSRLRRRPPAWSGLDREGRIVALYGILAVLWLAIAANLAYRIWADRIAGLVTGLWHSGFAAKVLLLLIVLGLCTPLVYLAVGRAARWWRTSRERATEKKREADAPRRLAALRASDLGGLPEPALAGLASRARWLRPSSGTQLVVAGATQQAVYVVVDGALQARKPGDPGGIIRHHVGAGGVVGLANALTGRSTSLDWHTAGTTLLSVPTATVATVVGPLPGPPPADRAEAEALFADTPALAGLAGDERLALIASAHPVDLEPGAPVVLSGPTHAIVVESGVIAMPDGVELRRGTLVGPVGDGTPGMVAQTRTPVRLWVIPDASDLPPLVGTPGRLVDGPGASGPIAAGLHSAGGYPPLAVPPGPPDGTEDPEVDRRFERKMWWLVLLLLLLALLLTAVNFAPGPAWAEMPTNRALLTSDRGTITATIDGRTETIGEDDRRYVDEGARITVPQDATGRLTFQGGSATLFCAGSSAQIGRLYTDAGRRRVPHGALTLDAGRVLADTASTSGAYQPLELAVARPAGLITNSGAAWYAAEPTGLSVATGRVYVTGTPSLPSGTDLSCGDGVKVEPPAGSPSPSPSDEPLPSDLPTEATPTETPSTIPPTTEATADPEPTLDPVEPTTPDAPPPTTTRPTTRPTTRPPATTPTRTTPPAPTPTRTTPPVTTPPTQTTPPTIPPTTTTPPIVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEY0</accession>
    <name>U5WEY0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562838.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41260"/>
    <dbReference type="EnsemblBacteria" id="AGZ46506">
        <property type="protein sequence ID" value="AGZ46506"/>
        <property type="gene ID" value="AFR_41260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8350"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046423_0_0_11"/>
    <dbReference type="OrthoDB" id="1421503at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="358" mass="37601" checksum="31511D0152B47C83" modified="2014-01-22" version="1">MSTEPTSQSEPETTPEEYRQRALLMADLGRYDEAVGEIAAGLAVAPDEPGLLSTLARVHVIAEQPVDALAAADRAVAAAPGTIGPLVVRAMALSDDRRYSEAAQVAHSILTTWPEDAYAQRTGAALLSESRNGQDALNAAWAGVRLTPADAEAHLVLAVVAARLRLFELAQRAYGEALDLDSAIADAQRDVGVVRLERRRWALALENLADAEIQVPEPVAPPTPVADVSRPARSVLDRSASTVETFRQAVLYGSNGVLIAAVLAAVMTLASSGVARMWAGVIGVVVLVALGVWLSRSLHEPVAAALRRLPRRLSFACYATAVAPLGLLGFALIGGVVPLILAMVVAAVAELVVLTQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFA7</accession>
    <name>U5WFA7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562922.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41685"/>
    <dbReference type="EnsemblBacteria" id="AGZ46591">
        <property type="protein sequence ID" value="AGZ46591"/>
        <property type="gene ID" value="AFR_41685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8436"/>
    <dbReference type="HOGENOM" id="CLU_2713244_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="72" mass="7863" checksum="ECCF68DC216E8D88" modified="2014-01-22" version="1">MFDLEFLKANLWAIAILLGLCARPAADVTTFFCAMVFVEKSQRLEAIKAIRAGQAPRTDTPPTPESPPWASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VND2</accession>
    <name>U5VND2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357421.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00945"/>
    <dbReference type="EnsemblBacteria" id="AGZ38478">
        <property type="protein sequence ID" value="AGZ38478"/>
        <property type="gene ID" value="AFR_00945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.188"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_12_0_11"/>
    <dbReference type="OrthoDB" id="1282004at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="29276" checksum="1FECBD484B677AD7" modified="2014-01-22" version="1">MPTISTSDGVNLHYTDEGSGRPVVLIAGFTAPTESWELQRLALLATGYRVLALDRRSHGLSDNPAYGQRMSRHGKDVRDFLDAAGLDDVVLIGGSMGASTIWAYYDLFGADRVRGIVSVDQTPKMVNDEVWKNGFYGLDRHNLGTFFEDGVPDTGRGLSVTESMRRIGRLIETLGRVPQMSDARAPETWPLLQDHAEQDWRDVVARVSVPSLFIAGADSQLWPSEHATDAAATNSRAASLVLDDCGHAANIDQPDLVNEAIVKFLAGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNF6</accession>
    <name>U5VNF6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357276.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00215"/>
    <dbReference type="EnsemblBacteria" id="AGZ38332">
        <property type="protein sequence ID" value="AGZ38332"/>
        <property type="gene ID" value="AFR_00215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.44"/>
    <dbReference type="eggNOG" id="COG2170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029030_0_0_11"/>
    <dbReference type="OrthoDB" id="367541at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042398">
        <property type="term" value="P:cellular modified amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016602">
        <property type="entry name" value="UCP012666"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012666">
        <property type="entry name" value="UCP012666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="491" mass="54277" checksum="A9AFB46957F0459B" modified="2014-01-22" version="1">MGKDLSGVVIAQDDRVHYRHKVRRCLEVLSSMLGNSAFDADSPTTGLEIELNLMDADAEPAMRNAEILANLADPTFQPELGQFNLELNARPRLISGNGFADYEDDILDSLARVEDRAKKSDSTIVLIGSLPTLRPAHLVLANLSANERYRALNDQIVGARGEQFSVDINGVERLQTTNDSIAPEAACTSVQCHLQVPPDRFAAYWNASQAVAGVQVAIGANSPYLHGRELWAETRIALFEQATDTRTDELKAQGVRPRVWFGERWITSVTDLFEENARYFPPLLPIIDDEDPLAVLEAGGVPRLGELRLHNGTVYRWNRPVYDVMNGLPHLRVENRVLPAGPTVVDMLANAAFYFGMIRELAEEDRPIWETLPFTDAEDNFHAAARRGIEASLAWPGLGTVPVTRLVLDVLLPKAYAGLDRFGVDPGLRDRLLGVIEGRCGSGRNGAWWQVGAVRRGEGRGLSRDAALHAMLLRYRSLQALNCPVHLWPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNW4</accession>
    <name>U5VNW4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH-quinone oxidoreductase subunit I</fullName>
            <ecNumber evidence="5">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NADH dehydrogenase I subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NDH-1 subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nuoI</name>
        <name evidence="8" type="ORF">AFR_01695</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357571.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01695"/>
    <dbReference type="EnsemblBacteria" id="AGZ38628">
        <property type="protein sequence ID" value="AGZ38628"/>
        <property type="gene ID" value="AFR_01695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.341"/>
    <dbReference type="eggNOG" id="COG1143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067218_4_0_11"/>
    <dbReference type="OrthoDB" id="1619561at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="PTHR10849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="24002" checksum="BB44260C96BD82CD" modified="2014-01-22" version="1">MGTFTGSFKGFGVTFAHMFRKVITTDYPFTPPTPAPRYHGRHILNRHPDGLEKCIGCELCAWACPADAIYVEGGDNTDEQRFSPGERYASIYQINYARCIFCGLCIEACPTRSLTMSNEYELARDSRQDLIFTKKELLAPLLPGMEQPPHPMRLGETDKDYYLGALTNPGTSAGAERVPSSERGTDDASTAGTPGDRPETAGTLRRDDAADQPKEVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP45</accession>
    <name>U5VP45_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative response regulator receiver domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357492.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01300"/>
    <dbReference type="EnsemblBacteria" id="AGZ38549">
        <property type="protein sequence ID" value="AGZ38549"/>
        <property type="gene ID" value="AFR_01300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.260"/>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_1_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="6"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="20288" checksum="0274A548EDEAFD64" modified="2014-01-22" version="1">MAERAKALLVDDRRDNLLALEAILQGLPVQAVAVESGEAALKQLLVDDFAVILLDAQMPNMDGFETAGHIKRRERTRHVPILFLTAADRDAQLALRGYAVGAVDYLTKPFDPWVLRAKVSIFVELWTKTQQLKAQAEATRERETQWQRLTDLVDEAARTLRAGGDEAAAEALDLLEKARWGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP73</accession>
    <name>U5VP73_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_02395</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357711.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02395"/>
    <dbReference type="EnsemblBacteria" id="AGZ38768">
        <property type="protein sequence ID" value="AGZ38768"/>
        <property type="gene ID" value="AFR_02395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.484"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="12"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="517" mass="55734" checksum="B952AF8C6C5FE2A4" modified="2014-01-22" version="1">MNAAQDLVGGRYRLLEVIGAGGMGRVWLADDELLRRRVAVKEIGTPTDVSTSELLDLQLTTMREARATARLDHPGVVGVFDVVWRPDRSWIVMEYVESRSLQDVVRENGPLPHREVARIGLNVLGALRVAHAAGVLHRDVKPHNVLLATDGRVVLTDFGLAKLEGIDQGPEPLLGSPHFVAPERLSGVESSPASDLWSFGAMLYAAVEGRAPFQRGTATASLTALTKQERPDGAQHPGPLTPIIDGLLVKDPAKRMTAEDAEDRLRRVADRAVGVFPMHIPRPGTAAAIASALGRSIPARPARRRIARSTKIALLAAAVLLVGTTGTALALDVRPGSFPGTTPSADAVSAMAAVCAAGGQAVSGTTDNRGYAVPAGWLWHSDPNGFDVAVPQGWTRSQDGTTACFRDPGGSRSFRVDATARLTGTALAYWQDQEQTALTERSLPGYTRVAMTTLTQKQGGADWEFTWQPPGEPVRRHERRVLLSMAPDRTYLVQWTTRDQDWTSNEAYLELILASLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPP8</accession>
    <name>U5VPP8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Yd repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357730.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02490"/>
    <dbReference type="EnsemblBacteria" id="AGZ38787">
        <property type="protein sequence ID" value="AGZ38787"/>
        <property type="gene ID" value="AFR_02490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.503"/>
    <dbReference type="eggNOG" id="COG4372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_223013_0_0_11"/>
    <dbReference type="OrthoDB" id="646440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028908">
        <property type="entry name" value="Tox-PL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15644">
        <property type="entry name" value="Gln_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln_amidase" evidence="2">
        <location>
            <begin position="147"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1161"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1444"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1862"/>
            <end position="1881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2116"/>
            <end position="2187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2514"/>
            <end position="2543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3022"/>
            <end position="3043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3067"/>
            <end position="3105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3123"/>
            <end position="3146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3465"/>
            <end position="3518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3552"/>
            <end position="3619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3752"/>
            <end position="3772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="4036"/>
            <end position="4065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="4372"/>
            <end position="4399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="4569"/>
            <end position="4605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="4882"/>
            <end position="4929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1176"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3022"/>
            <end position="3038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3476"/>
            <end position="3518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3556"/>
            <end position="3586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="4043"/>
            <end position="4059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="4575"/>
            <end position="4594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="4899"/>
            <end position="4927"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="6085" mass="660038" checksum="936959A644B3CB7F" modified="2014-01-22" version="1">[encoded data, 6085 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ66</accession>
    <name>U5VQ66_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HATPase_c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03845"/>
    <dbReference type="EnsemblBacteria" id="AGZ39058">
        <property type="protein sequence ID" value="AGZ39058"/>
        <property type="gene ID" value="AFR_03845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.777"/>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090336_4_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HATPase_c" evidence="1">
        <location>
            <begin position="16"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="12702" checksum="CC540E7EBC882384" modified="2014-01-22" version="1">MLGDLPSAGTACGIGRRFVAAQLRGWQVDESIVEVAVLITSELVANAVNHAPPPGHLQVSLDGRRVRIEVTDASPLPPEIVSPGALATGGRGLLLIGRLASGWGWEPQAPGKVVWCEVILPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQC2</accession>
    <name>U5VQC2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Monocyte differentiation antigen CD14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357957.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03615"/>
    <dbReference type="EnsemblBacteria" id="AGZ39012">
        <property type="protein sequence ID" value="AGZ39012"/>
        <property type="gene ID" value="AFR_03615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.728"/>
    <dbReference type="eggNOG" id="COG4886">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054192_0_0_11"/>
    <dbReference type="OrthoDB" id="1499307at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="311" mass="33269" checksum="741C041D9164D741" modified="2014-01-22" version="1">MTINSHVTTFAGLPVVAFDPEGELPDDPAAVAWRVETEDFEAPPEEFAGLIEALITRVPEGQIRALVLGEWGSTYEVGPPIELLISLAPRLSGLRAIFVGDLTYEQCEISWIHQGEITPLLTAYPELEVLRIRGAEGLVFEAAQHASLRELGFESGGLPAQVVRAVGASDLPALQSLDLWLGTSNYGADSTVDDLAPILSGERLPSLRSLALCNAEIADAVAAAVATAPVIARLDVLDLSKGELTDEGLDALLAGQPLTHLSRLDLHHHYLSAEGQERLTAALPGVEIDLSEPRESDESDGRVFRYVSVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQC5</accession>
    <name>U5VQC5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358073.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04195"/>
    <dbReference type="EnsemblBacteria" id="AGZ39128">
        <property type="protein sequence ID" value="AGZ39128"/>
        <property type="gene ID" value="AFR_04195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.847"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077925_0_0_11"/>
    <dbReference type="OrthoDB" id="1457558at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="60"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="32747" checksum="22690B7E96E123F6" modified="2014-01-22" version="1">MTFTPRTRWADAPDSIRTAVAEALGSAVVDHTDLHGGMSPGPAVRLRLADGRQSFVKAVSAEVRAHNHRMIAQESAILDILPPSVSAPRRLATVERGPWIALATTWATGTTPELWTDVSIEAVARACRTANGHEAPRTLPPVVQRIFDFDGWLRVRELGTADDWEASHAGPAAEVVAGWERWTAGDALVHRDLRLDNTAVDAEAGSAVLLDWAYASAGAPWIDLAQLAADVVATGHASGERVATDRAYGLLGTLPPEASRFVVALAGMWSIRAATVTSSVMPGIAAWRRARTAALRPLVSRLIADLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRC3</accession>
    <name>U5VRC3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Type-5 uracil-DNA glycosylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_05365</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 5 (UDGb) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05365"/>
    <dbReference type="EnsemblBacteria" id="AGZ39362">
        <property type="protein sequence ID" value="AGZ39362"/>
        <property type="gene ID" value="AFR_05365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1086"/>
    <dbReference type="eggNOG" id="COG1573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083279_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033958">
        <property type="term" value="F:DNA-deoxyinosine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10031">
        <property type="entry name" value="UDG-F5_TTUDGB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044147">
        <property type="entry name" value="UdgB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="10">
        <location>
            <begin position="65"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ39362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="26299" checksum="57E96ECE76E2BC33" modified="2014-01-22" version="1">MLGAVLSRTPEEVSAHAAAAADLTAVDAGVADCFACPRLVAWREEVAVVKRASFRDQDYWGRPVPGFGPADAPIAILGLAPAAHGGNRTGRIFTGDRSGDVLFAALHRAGLANQPTSVSADDGLALHHTRIFAAVRCAPPDNKPLPVERDTCAPWLHRELALIRPTLRVVVALGAFAWAAWWPAMTAVYGHKPPVPRPAFGHGAQVSVPGTPDLLGCYHVSQQNTFTGRLTPAMLDDVFASAKRLAGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRH9</accession>
    <name>U5VRH9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative ATP-dependent DNA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_06270</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359074.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06270"/>
    <dbReference type="EnsemblBacteria" id="AGZ39543">
        <property type="protein sequence ID" value="AGZ39543"/>
        <property type="gene ID" value="AFR_06270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1272"/>
    <dbReference type="eggNOG" id="COG1793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008325_2_0_11"/>
    <dbReference type="OrthoDB" id="1073940at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014144">
        <property type="entry name" value="LigD_PE_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13298">
        <property type="entry name" value="LigD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02777">
        <property type="entry name" value="LigD_PE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LigD_N" evidence="3">
        <location>
            <begin position="37"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_LIGASE_A3" evidence="2">
        <location>
            <begin position="165"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="355" mass="40094" checksum="A0465214A9CF5283" modified="2014-01-22" version="1">MTDQLEEYRRRRDARRTPEPVPRARPRPTTGNRFVIQQHHARSLHWDVRLERDGVLVSFAVPRGLPRDRARNHLAKHTEDHPLEYLDFAGEIPAGEYGGGRMTIHDRGTFETDKWRDDEISVTFHGERTSGRYVFFQTGGNDWMVRRMDPSEPGWETMPEAVEPMLATRAAGLPPDDQDWGYELSWAGRRTLIQVSGGRVKVTDAEGTDVTSWYPELRPLGEALAPLEAVLDGEIVAFDGARVSPELLERRKAPKDSAAARRAAERTPVQFLAYDLLWLEGHSTVELVRYAERRELLEGLDLAGDNWQTPPYFSGGGSFALDAAQAQGLDGVVAKRLDSPYLPGRRSRLWLSVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRU3</accession>
    <name>U5VRU3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative branched-chain alpha keto acid dehydrogenase E1 beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00485"/>
    <dbReference type="EnsemblBacteria" id="AGZ38386">
        <property type="protein sequence ID" value="AGZ38386"/>
        <property type="gene ID" value="AFR_00485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.99"/>
    <dbReference type="eggNOG" id="COG0022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="5"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="35458" checksum="CBD3D0E4DAC9403A" modified="2014-01-22" version="1">MTETLTLGKALNHGLRRALENDPKVVIMGEDVGKLGGVFRITDGLQKDFGEDRVIDTPLAEAGIIGTAVGLAIRGYRPVCEIQFDGFVFPAYNQIVAQVAKMYYRSLGNVRLPIVIRIPYGGGIGAVEHHSESPEAYFAHTPGLKVVTCSNPADAYAMIQQSINSDDPIVFFEPKRRYWEKGAVDLDTPSTFPLHASRVAREGSDATVIAYGPMVRTALDAAQAAAEDGRTLEVIDLRTLSPLDLGPVYDSVRKTGRAVVVHEAPGNLGLGAEIAARITEECFYSLEAPVLRVTGFDVPYPSARVEEEYLPDLDRVLDAIDRSLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRW7</accession>
    <name>U5VRW7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357428.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00980"/>
    <dbReference type="EnsemblBacteria" id="AGZ38485">
        <property type="protein sequence ID" value="AGZ38485"/>
        <property type="gene ID" value="AFR_00980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.196"/>
    <dbReference type="eggNOG" id="ENOG5032SHV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106273_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009937">
        <property type="entry name" value="Phage_holin_3_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07332">
        <property type="entry name" value="Phage_holin_3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="13980" checksum="1D1E7D678B0D5C9A" modified="2014-01-22" version="1">MSSDPPQSAESTSDLVSQAAAQISTLVRDELTLAKLELAEKGKRAGIGGGLFGGAAVLGMYALGLLLALAVVLLDLVWPLWLALLVVLVVVVAAAGIAALVGKQKLQAAVPPVPTDAVASTQADVKTVKNAVREGRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRX4</accession>
    <name>U5VRX4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07195"/>
    <dbReference type="EnsemblBacteria" id="AGZ39728">
        <property type="protein sequence ID" value="AGZ39728"/>
        <property type="gene ID" value="AFR_07195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1468"/>
    <dbReference type="HOGENOM" id="CLU_851604_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="37767" checksum="9A9A57899A5657E6" modified="2014-01-22" version="1">MVPGLLLSVSIVSYLLLYTLRETIKLQTSLPVLDRIWSDVTTTVLLAGIVLATWERSSRKDMERFVRRLSQEQREISEKMLESVLERGSVRAMLAEKITEPVFLSIQRQIIDVPVVNRDYRLSLKIHRLAADPDFVSIKIFERYRLCNTSPESRVLDLHCMSDFNYEDRFPGHGRLTEVRLQPNRLRDRLRLDGVGSIDDKTLDETLLAEYAERLGSALEYHIPTPRVDPGDEIMIMVRRTKIQRANDVNFWRVLQATENVELDIEHDEELEVKAFQSAPWRAFSKQDHSDRTQAIWTMNHAILPHQGFDISWRVKSATPDEADAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS79</accession>
    <name>U5VS79_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bac_luciferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357493.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01305"/>
    <dbReference type="EnsemblBacteria" id="AGZ38550">
        <property type="protein sequence ID" value="AGZ38550"/>
        <property type="gene ID" value="AFR_01305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.261"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_6_3_11"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="266" mass="27918" checksum="8F2399BED29B1E98" modified="2014-01-22" version="1">MSFRFGVVAGRARTGAEWITTAQRAEELGYDSLLVPDTLHTLSPFAALAAAATATTTLRLGTYVLSAPNRSPGLVAWETRSLQLLSSGRFELGIGGGRPGAERDAAALGGEFGSPGERLRRVAETIRQVREDPDAPPVLVAASGPRMLRLAVEQADIVALGLPPQTTEEELAATVAGLGNSPAELHVNVAAVAESVDGIPEWLSRMVGGDPREMAAAGGIAFLIGTAEQIADKLLRRRDELGISYIGVSGLFLEQFAPVLKLLRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSB8</accession>
    <name>U5VSB8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01555"/>
    <dbReference type="EnsemblBacteria" id="AGZ38600">
        <property type="protein sequence ID" value="AGZ38600"/>
        <property type="gene ID" value="AFR_01555"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01555"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.312"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_34_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15401" checksum="0841394B3EE4E309" modified="2014-01-22" version="1">MNDLVLRAADSRELAAVLTFWATAAENASRPPDSLAAVAALHLRDADALILAVEEDRIIGTIIAGWDGWRCHLYRLAVHPERRRAGIGGALIAAAEERARALGGTRMDAMVLDDNDQAHAIWAANGYHRQEDWSRWVKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSQ1</accession>
    <name>U5VSQ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357688.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02280"/>
    <dbReference type="EnsemblBacteria" id="AGZ38745">
        <property type="protein sequence ID" value="AGZ38745"/>
        <property type="gene ID" value="AFR_02280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.461"/>
    <dbReference type="eggNOG" id="COG4942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073856_0_0_11"/>
    <dbReference type="OrthoDB" id="1180964at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665706" evidence="2">
        <location>
            <begin position="34"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="359" mass="38015" checksum="37E4DA1F25F95670" modified="2014-01-22" version="1">MRLKPARRRLATVLAALVATTGLALVAPSSASASPRPLAAPGDSGDDGEGGSKSLVQQLEAASKGFVEAKEALATSRKRQQQLIDKLKEINTELGPKQAALDDIVQQSYRTGRLGPMTALVTADSTGSMLDRVQTLETIAVKQDRAVRDLKDSKTQQEQAKVAIDAEIRDQQRQLTVMAKRKTQAENALKAANAASGSSDASDSSGGSSSSDGGSSSKAAAAPRNSNGELPNESCSINDPTSSGCITPRTLHAMKEAKKDGFTRFVACFRQQNSGEHPKGRACDFAADKNGFGGDATGSSKTYGNQLANYFINNASRLGVLYVIWFRRIWLPSSGWKSYSGAGGDPSSDHTNHVHLSMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSX2</accession>
    <name>U5VSX2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_02285</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357689.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02285"/>
    <dbReference type="EnsemblBacteria" id="AGZ38746">
        <property type="protein sequence ID" value="AGZ38746"/>
        <property type="gene ID" value="AFR_02285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.462"/>
    <dbReference type="eggNOG" id="COG1134">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="887953at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03220">
        <property type="entry name" value="ABC_KpsT_Wzt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015860">
        <property type="entry name" value="ABC_transpr_TagH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="38"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="75"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="28040" checksum="EBE791D2CA4AECEB" modified="2014-01-22" version="1">MADVSSNDRRDPTIIVDDAHVVYRVHQSATPGTGSPLASFKRIVTGAKPATLREVHAIKGVSFVAYKGEAVGLIGTNGSGKSTLLRAIAGLLPVEKGAIYAAGQPSLLGVNAALMNDLPGERNVVLGCLAMGMSPAEVKAQTKDIIEFSGINERGDFSSLPMRTYSSGMAARLRFSIAAAKKHDVLLIDEALATGDAKFRRRSEQRVRDLRAQAGTVFLVSHSEQSIRDTCERAIWLESGLIRADGPTDEVLKEYEAYTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT20</accession>
    <name>U5VT20_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.42</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_09070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.42" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359652.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09070"/>
    <dbReference type="EnsemblBacteria" id="AGZ40103">
        <property type="protein sequence ID" value="AGZ40103"/>
        <property type="gene ID" value="AFR_09070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1845"/>
    <dbReference type="eggNOG" id="COG1391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006233_1_0_11"/>
    <dbReference type="OrthoDB" id="427701at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008882">
        <property type="term" value="F:[glutamate-ammonia-ligase] adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023057">
        <property type="entry name" value="GlnE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005190">
        <property type="entry name" value="GlnE_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013546">
        <property type="entry name" value="PII_UdlTrfase/GS_AdlTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30621">
        <property type="entry name" value="PTHR30621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08335">
        <property type="entry name" value="GlnD_UR_UTase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03710">
        <property type="entry name" value="GlnE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GlnE" evidence="3">
        <location>
            <begin position="163"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="GlnD_UR_UTase" evidence="4">
        <location>
            <begin position="350"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="GlnE" evidence="3">
        <location>
            <begin position="601"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="GlnD_UR_UTase" evidence="4">
        <location>
            <begin position="869"/>
            <end position="1005"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1011" mass="109687" checksum="104B5FE03EA0C7A8" modified="2014-01-22" version="1">MTRPSRLARYGFADNGTRAADLLGPGGLRLWDVEAQGPVDADAGELIHALSRAADPNLALRQLHRLIDSVARAAHRANGGPVIGPGEDIVESPAADAVLHALYADPGLRRRLVAVLGASSALGDHLVANHDQWRVLATGKTGLPPNAEGHLEFGESMAEGPPTVPALRTAYRISLLRIVAADLTGGRGLEPTMAALSRLADETLASAYAIAVAGLPAGTPEPRLAVVAMGKCGGYELNYVSDVDVIFVAAGDDDLSAGTTVAARLIEVCGQVAWPVDAALRPEGNRGPLVRTLASHQAYYRRWARTWEFQALLKARPAAGDLPLAAEWIDDLAPLVWHAAERPEAVADVRDMRRKIIDNVPPREVDREIKRGPGGLRDIEFAVQLLQLVHGRIDETLREPGTLPALRALVAGGYVGREDGETLLRGYRFLRSIEHRLQMQNLRRTHTVPDDPKALRWLAAALGYRALPGRDAVEAFRSDWVGHASHVRRLHVKLLYQPLLEAVARVPAEALRMTTESAGRRLEILGFADPAGALRHLQALTGGVSRTAAIQRTLLPMLLQEFADAPEPDRGLLNYRHVSDKLGSTPWYLRLLRDGGPVARRLARVLSLSRYATDLLTRDPEALRLLADDAELQPRPRDVLRDGFIAAADRHLRDGTTDPAKAITAVRALRRRELFRLACADILSRAGDLAPAHPLDVHAVGIALSDVTDATLGAALHAARRVHPGPDGLRFAVIGMGRLGGYEMSYPSDADVLFVYAPPEGMSDGEASASAHAIAEEMRRTLSAPAPDPALGIDADLRPEGRQGPLVRSLNAYAQYYARWSKVWESQALLRARFVCGDEGLGREFEELADGMRYPSGGLTREQVVEIRRIKARVETERLPRGADPNTHTKLGRGGLADVEWAVQLLQLRHGYALPALRMTRTLEALSAELDAKLVDKVDAAAMTAGWTLAAQVRNALTLVRGRPTDQLPRHGVELAGVVQLLGGGDPGEFVDRYLRITRRSRAAMERVLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTA4</accession>
    <name>U5VTA4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chitinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_03010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357834.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="SMR" id="U5VTA4"/>
    <dbReference type="STRING" id="1246995.AFR_03010"/>
    <dbReference type="EnsemblBacteria" id="AGZ38891">
        <property type="protein sequence ID" value="AGZ38891"/>
        <property type="gene ID" value="AFR_03010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.605"/>
    <dbReference type="eggNOG" id="COG3325">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002833_14_4_11"/>
    <dbReference type="OrthoDB" id="1081028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="SSF54556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665183" evidence="5">
        <location>
            <begin position="27"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="6">
        <location>
            <begin position="34"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="401" mass="41616" checksum="8318FCF3ECFB72C3" modified="2014-01-22" version="1">MRVLPVILSAVLLLAAGCDSSPPAKPAPQTADQQRVVGYFTDWGVYGRNFQVRNVDASGAAADLTHLMYAFGKLDGGKCTTSDKWADYEKPIAAADSVDGVADTPGAGLRGNFGQLRKLKAAHPRLKVIWSFGGWTGSKGFTEAAKDPAAFANSCYKLLTDPRWSGVFDGIDVDWEYPNACGLVCDTSGPDALPGLLKSLRSTFGPEALITAAVPGDLGKLKATDYAAAARSADWLSAMTYDFFGTGGAGTGPTAPHSPLKAYPGIPRETSTTDAAISGLLGLGIPPEKLLLGIGFYGRGWTGVSQAGPGGTASGPAQGSYDKGIEDYAVLQETCPPTGSIGGTSYAVCGGQWWGYDTPETIKAKMAYARSKALGGAFAWELSGDTDDARLVTAMKDGLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTC0</accession>
    <name>U5VTC0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357928.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03480"/>
    <dbReference type="EnsemblBacteria" id="AGZ38985">
        <property type="protein sequence ID" value="AGZ38985"/>
        <property type="gene ID" value="AFR_03480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.699"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_1_1_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="24"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="146" mass="15400" checksum="7A712A95A098772F" modified="2014-01-22" version="1">MASRNSLEAVYREYLAALVLFHQSAAHAVGLGPTDYQALNLLELRGPQTPGALSAALGLTTGATTRLVDRLVAAGYAERAADPHDRRKVTVTATMTPEDLAELLDGVRGSIAGYVSTLDEAQRDVLVGYWQAAGQGYAKGLSLLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTE5</accession>
    <name>U5VTE5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HEPN_SAV_6107 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09870"/>
    <dbReference type="EnsemblBacteria" id="AGZ40263">
        <property type="protein sequence ID" value="AGZ40263"/>
        <property type="gene ID" value="AFR_09870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2013"/>
    <dbReference type="eggNOG" id="ENOG5032S47">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096112_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040891">
        <property type="entry name" value="HEPN_SAV_6107"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18726">
        <property type="entry name" value="HEPN_SAV_6107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN_SAV_6107" evidence="2">
        <location>
            <begin position="56"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="17642" checksum="1D5AB0AA3265A9EE" modified="2014-01-22" version="1">MVSNPAYPVPRQQGPGPLAHAPTAAAQTAPTVPANLLPNRTPAQLLAIARQGLAEAARTRPDGLRYATAHLAALRAAAAVLAARARPAAPTKRSRVTSAWSLLVLVAPEFSDWAGYFAQGATKRAAAEAGIPRVVTEREADDLLRAAEHFVSLVESVLGLAYQPTLEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTH2</accession>
    <name>U5VTH2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF1232 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08940</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08940"/>
    <dbReference type="EnsemblBacteria" id="AGZ40077">
        <property type="protein sequence ID" value="AGZ40077"/>
        <property type="gene ID" value="AFR_08940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1819"/>
    <dbReference type="eggNOG" id="COG3339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130372_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010652">
        <property type="entry name" value="DUF1232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06803">
        <property type="entry name" value="DUF1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1232" evidence="6">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="159" mass="16974" checksum="77324D44B08CE7EF" modified="2014-01-22" version="1">MAATGAARRAGAWAGGAVAEGRVGMAWWWQALISVGVALLLAWVALVIALLVVKPDAGRLKEALRLLPDLLRLLRRLAADRALPRGVRVRLGLLLAYLAMPFDLIPDFVPVLGYADDAIIVIAVLRSTVRRAGIEAVRGHWPGTEEGFAALSRLAGLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTT6</accession>
    <name>U5VTT6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase H</fullName>
            <ecNumber evidence="6">2.1.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA m(4)C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">rRNA (cytosine-N(4)-)-methyltransferase RsmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmH</name>
        <name evidence="8" type="ORF">AFR_09975</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10287"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74506"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.199" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09975"/>
    <dbReference type="EnsemblBacteria" id="AGZ40284">
        <property type="protein sequence ID" value="AGZ40284"/>
        <property type="gene ID" value="AFR_09975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2033"/>
    <dbReference type="eggNOG" id="COG0275">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038422_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="PTHR11265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004486">
        <property type="entry name" value="MraW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="SSF81799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00006">
        <property type="entry name" value="TIGR00006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="43"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="321" mass="34731" checksum="C7279BAC25ADE00A" modified="2014-01-22" version="1">MGVDMGELRGTHVPVLLERCLELLAPALVLPGAVHVDFTLGLGGHAEAVLERYPDVVLIGLDRDTEALAHSRHRLARFGDRVHFVHAVYDELPDVLAGLRLPAFHSGLFDLGVSSLQLDEPDRGFAYSRDAPLDMRMDQSRGITAEEVVNGYEPGELVRILRVYGEEKFATRVVSAIVKQRSKSRIVSSSALADLVRDAIPAAARRTGGNPAKRTFQALRIEVNAELAAVESAVPAALDLLAPKGRLVVMSYQSLEDRIVKRALTARARSTGPIDLPVELPGTGPSVRLLTRGSEPPSEAEVAENPRAASVKLRAAEKIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTV2</accession>
    <name>U5VTV2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">IclR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_10720</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10720"/>
    <dbReference type="EnsemblBacteria" id="AGZ40433">
        <property type="protein sequence ID" value="AGZ40433"/>
        <property type="gene ID" value="AFR_10720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2185"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_6_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="57"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="238" mass="25409" checksum="FD572A212447249F" modified="2014-01-22" version="1">MLAVLTELAQHGDGVSLEEMTQAVASPKPTVHRALASLCRFGFAARNGHGRYVLGDEFLRLAFAHHEARPDHLRIAPVLTELARRYGETAHYAVLDGDTVVYRAKVDPPAGALRLTSTVGGRNPVHSTAVGKLLLSYALSDEPAVADWIGDRPLTRRTPHTLTTAEALAADLARIRVAGYAVDDQENEPGIVCVAVPVFMTSPTRPSGAVSVSAPALRTSLETLLAQIPAIRATVEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTW8</accession>
    <name>U5VTW8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Lipid A export ATP-binding/permease protein msbA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_10795</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360331.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10795"/>
    <dbReference type="EnsemblBacteria" id="AGZ40448">
        <property type="protein sequence ID" value="AGZ40448"/>
        <property type="gene ID" value="AFR_10795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2200"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_9_11"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="47"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="385"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="419"/>
            <end position="426"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ40448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="649" mass="70013" checksum="3E78A914F47191CA" modified="2014-01-22" version="1">MGPMSSGSSWMMLRSIQNSERVSKHQLSRGTTKRILKFARPYRREIIVFLITVVFAAGIGVATPVLAGHVVNAITAGGSDAAATVIKLAFTIAGLAVIDAFLSLAQRWYSARIGEGIILDLRTRVYDHVQRMPLQFFTRTQTGALVSRLNNDVTGAQRAFTSTLSGVVSNVIQLVLTAGVMFSLSWQITVLSLVMLPVFIIPARRVGKKLAEITRESYNLDAKMNATMTERFNVSGALLVKLFGRPDTEAAHFGERAQRVRDIGVQQAMFSRTFFVAMLLVASLAQALTYGLGGWLAVTGSVTAGTVVTLALLLTRLYGPLTALSNVRVDVMSALVSFDRVFEVLDLKPGIEEKPDAVAIPAGSGRLEFRDVHFRYPSADEVSLATLEDVVALDRTENAPVLRGVDFTVEPGQLVALVGPSGAGKSTTSMLVSRVYDVTEGAVRVGGVDVRDATLDSLRDTIGVVTQDSHLFHETIAENLRYARPDATEADMWEALHGAQVAALVRALPDGLDTVVGERGYRFSGGEKQRIAIARLLLKHPSIVILDEATAHLDSESEAAVQRALSVALKGRTALVIAHRLSTVRDADQILVLDDGRIVERGKHNELVAAGGLYAELYRTQFAVADSPAQPFADDPEPVIVTAAHPVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUF6</accession>
    <name>U5VUF6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05355"/>
    <dbReference type="EnsemblBacteria" id="AGZ39360">
        <property type="protein sequence ID" value="AGZ39360"/>
        <property type="gene ID" value="AFR_05355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1084"/>
    <dbReference type="eggNOG" id="COG4709">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_856944_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="35411" checksum="F4B8152563AF2A17" modified="2014-01-22" version="1">MITTAQDEINLYVFAVRAALVDLPESLREELLEDLPEHLAEVLADGGGTLVERLGSPEAYAADLRASVGFVSGFPDPPTRRHQFYEVRDQAMLRLGQADRKLGPLLGYPKVSEFLVLLRPAWWVLRGYLAAMVLAWMLDDSGQPIGLLPRIGGSEAVALLLLGLCVVASIWLGRRSLRLTRWPRLALYAGSVILVLVALGGFSSADSSSRDSGYTDVNYDNPYGNVEDLFVYDEQGRLVTNARIVDQNGEPLRMGNQYCYDEAGTYEEVDTLTYPYCPERAPWRVPSAAGPSGAPSTTGGPGPLWEPSASPSATPTAGPTKTPTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUN8</accession>
    <name>U5VUN8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cation efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_12030</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360752.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12030"/>
    <dbReference type="EnsemblBacteria" id="AGZ40693">
        <property type="protein sequence ID" value="AGZ40693"/>
        <property type="gene ID" value="AFR_12030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2449"/>
    <dbReference type="eggNOG" id="COG0053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073293_2_0_11"/>
    <dbReference type="OrthoDB" id="381612at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026765">
        <property type="entry name" value="Tmem163"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31937">
        <property type="entry name" value="PTHR31937"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ40693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="21442" checksum="8E85970CF2B6C461" modified="2014-01-22" version="1">MELLQIQSSPRRALLARRVRLLVAATIAYNVVEAVIAISAGAAASSTALIGFGLDSVIEVSSAAAVAWQFAGRDPEAREKTALRIIAFSFFALAAYVSVESVRALATGSAAEHSRVGLVLAAVSLLVMPALSYAQRRAGRELGSRSAVADSKQTLLCTYLSGVVLVGLGLNSIFGWTWADPVAALVIAAVAVREGRTAWRGDHCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUN9</accession>
    <name>U5VUN9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_11300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11300"/>
    <dbReference type="EnsemblBacteria" id="AGZ40549">
        <property type="protein sequence ID" value="AGZ40549"/>
        <property type="gene ID" value="AFR_11300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2303"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="132"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="4">
        <location>
            <begin position="257"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="228"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="461" mass="47842" checksum="564B5B2B3CFFE14A" modified="2014-01-22" version="1">MPGSDRGRAGHGGDAGFDRGRPGHGGEADSDRGRAGHGGGSGFGRGAAGRGRGPGWAGTDRSRSWPPRRADGSPRQGRHAWPGAVVAALSFHAFGSGAEASALAHPTDIIAWVLLLAGPLALAWRGRAPIAVLIVSGAATIGFATLVEPAWTYAVAVVIALFTAVKAGRRAVAVPIAIAGYLAYLAVMWFFADALGLPAAVRPEVRDSVLTAVGLAMTVFVGNAARARSAYLAEMSKAKAERARAKEEQERRQASDERLRIARELHDVLGHHLSLINVQAGVGLHLMDNRPEQAREALAAIKTASAEALREVRAVLGVLRPEEEAAPRQPALGLDRLTDLTADAGLPVTTETVGQRRDLPAEVDRAAYRIVQEALTNIRRHADTGVAATVSVTYAETELHLSVQNDGLPAEDEPDGDDNGSGIAGMRARAASLGGTLEAGPLPGGGYLVSAVLPTGAGENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUZ8</accession>
    <name>U5VUZ8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359066.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06230"/>
    <dbReference type="EnsemblBacteria" id="AGZ39535">
        <property type="protein sequence ID" value="AGZ39535"/>
        <property type="gene ID" value="AFR_06230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1264"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090569_1_1_11"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="2">
        <location>
            <begin position="30"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="14003" checksum="9039E9670BA79E01" modified="2014-01-22" version="1">MEHFTIATVAEKNPDFRRVLWTGKHTQLVIMTIPPGGEIGEEVHEVDQILTFVSGVGQAKISGETRKVAAGDLVVVPAGRKHNFTNEGPNPLVLYTIYGPAEHAEGAVHHTKEEADRLEEAGKDEPPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV58</accession>
    <name>U5VV58_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13010"/>
    <dbReference type="EnsemblBacteria" id="AGZ40888">
        <property type="protein sequence ID" value="AGZ40888"/>
        <property type="gene ID" value="AFR_13010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2642"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_25_6_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011075">
        <property type="entry name" value="TetR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16859">
        <property type="entry name" value="TetR_C_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="19183" checksum="CB79EA17059F601E" modified="2014-01-22" version="1">MLHAADDLLAENGFAALTIEAIARRAGVAKQTIYRWWPSKVEILLDTLIEDSDKMVRVPMEHPTADLVRDYLRDFARFLTGDPAGRVYLALLAEAQHRPGVAEKLQERYLEPRRAQERDMLARGVQAGEFSPRLDADAAMDALTGPIVYCALIASVITDDLIDALVDGILRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVC3</accession>
    <name>U5VVC3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359186.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06830"/>
    <dbReference type="EnsemblBacteria" id="AGZ39655">
        <property type="protein sequence ID" value="AGZ39655"/>
        <property type="gene ID" value="AFR_06830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1395"/>
    <dbReference type="eggNOG" id="COG4954">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121942_1_0_11"/>
    <dbReference type="OrthoDB" id="1746879at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018988">
        <property type="entry name" value="DUF2000"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09391">
        <property type="entry name" value="DUF2000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="SSF102462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="16514" checksum="C32B6C3E7AA35E59" modified="2014-01-22" version="1">MDGINQVGFEPGEIRTDEPTRSARLKWVIVVDEELPAGRATNAAICVAAATTTAVTGLIGPEAKDAGGSVHPGLPWAGCSVLGASAARLAEIRARAVESLGVFVADMPTQGQSTRVYDEYLRQVGETAPDDLAYHAISLVGPRNRIDKLVHRLGLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVQ5</accession>
    <name>U5VVQ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07185"/>
    <dbReference type="EnsemblBacteria" id="AGZ39726">
        <property type="protein sequence ID" value="AGZ39726"/>
        <property type="gene ID" value="AFR_07185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1466"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="16289" checksum="FB8524BEA91A2352" modified="2014-01-22" version="1">MSTSDFRTQREAEIVEATRRMIAAAVLFSHKVAEQLKLGPSDAQFMTLLDVHGPLTPGRLSDLTGLKTGTVTGVLDRLESAGYVRRDRDPHDRRKVIVSLDPAQIAARVDPHYAGQAEHMTGLLQRYSDDQLALIADFMSTMSAEQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVU5</accession>
    <name>U5VVU5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alcohol dehydrogenase zinc-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361137.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13960"/>
    <dbReference type="EnsemblBacteria" id="AGZ41078">
        <property type="protein sequence ID" value="AGZ41078"/>
        <property type="gene ID" value="AFR_13960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2832"/>
    <dbReference type="eggNOG" id="COG1064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_0_0_11"/>
    <dbReference type="OrthoDB" id="884088at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADH_N" evidence="2">
        <location>
            <begin position="26"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="ADH_zinc_N" evidence="1">
        <location>
            <begin position="183"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="359" mass="37229" checksum="19932D74E182FB5F" modified="2014-01-22" version="1">MRAVILHEFGRPVEITTVADPVAGAGDVVVDVVATGVLPYTGEVLSGARRYALTLPVIPGPGAVGRVRSAGPDATRLAPGDWVLVDPTVRSRDGGLTPDITLQGWSARGDGGLVLQRYFGNGAFAEQVLAPAENVFPIGDIDPADAPRWVAAGVCLVPYGGLRAGELRPGETVLVSGATGNYGSIAIAVALAMGAGIVVAPGRNADLLDALVRRFGTRVRPVRLTGDEGADRAAMLAAAGPVDLVLDLLPPQAPAAAARAAAMTAREHGRVVLMGGVGMLGGDDLALPYPWLMRNSITVRGQWMYPPAANTELIRMVATGLLDLSLFDVTTFSLDETEKAVADAAAHARNFHLTTICPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVV3</accession>
    <name>U5VVV3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39776">
        <property type="protein sequence ID" value="AGZ39776"/>
        <property type="gene ID" value="AFR_07435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07435"/>
    <dbReference type="HOGENOM" id="CLU_1363784_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011049">
        <property type="entry name" value="Serralysin-like_metalloprot_C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101967">
        <property type="entry name" value="SSF101967"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="20850" checksum="4500EB9EA92B100C" modified="2014-01-22" version="1">MQARDGPTSLKLGSARAWGSPTSLRLGSRRPLEGPTSSKLGSRRLRDGLASLRLGSGRPLEGPTSSKLGSWRLRGGLASLRLGSGRPLEGPTSSKLGSWRLRGGLASLRLGSGQARDGPTSLRLGSGRPLDGPTSSKLGSGRPLDSPTSSKLGSGQPRDSPTSLKLGSGQPRDSPTSLKLECVRACAGFTSADSWWRHFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVV5</accession>
    <name>U5VVV5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase S1 and S6 chymotrypsin/Hap</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13280"/>
    <dbReference type="MEROPS" id="S01.101"/>
    <dbReference type="EnsemblBacteria" id="AGZ40942">
        <property type="protein sequence ID" value="AGZ40942"/>
        <property type="gene ID" value="AFR_13280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2696"/>
    <dbReference type="eggNOG" id="COG5640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_13_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="1">
        <location>
            <begin position="8"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="237" mass="26060" checksum="D7382F255650D65A" modified="2014-01-22" version="1">MAAPAAAISGGETAAPGEFPWAVRVEPAGCGGSLIHPQLVLTAQHCVDEQPGDSFTVVSGSVDLEDPRRTVTRSTAIIGGTAWWDGPDWAVIKLEKPLKLPTLRLAAKPRQLEKFTVVGWGRQKWEAPQERFLRKVELPYLPDEQCFDDPWWADHPEYPRAMICGKSVDQKSHCGGDSGGPAMHRLPNGTWEQIGIVSSGSDCDWDIGEEPRPNYTDVAYFSRDIQLAARRLLAGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW91</accession>
    <name>U5VW91_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_08210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359462.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08210"/>
    <dbReference type="EnsemblBacteria" id="AGZ39931">
        <property type="protein sequence ID" value="AGZ39931"/>
        <property type="gene ID" value="AFR_08210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1675"/>
    <dbReference type="eggNOG" id="COG1172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028880_2_2_11"/>
    <dbReference type="OrthoDB" id="1254678at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="358" mass="36993" checksum="9A258DDDF8751EBD" modified="2014-01-22" version="1">MTDQSTKPSLPAQSPPVAPAVSKDKSRANWLKGDGAESLRRNLGLVGVLVILVIIGIATRPELYGDATWVKNNVFTILTQASAIGVVTVGMTFVIIGGGIDLSVGAIIAIAGVWSTTLSTQSYGTFGMIFTAIVVGVVVGLVNGALISYGRLVPFIATLAMMVAARGLAAQISGKQTQVSANSTINSIASTKILGIPMLVIILAVVVAAGWVLLNRTTFGRRTVAVGGNPEAARLAGINVRWHTVLLYALSGLCCGIAAIMLTSQATSAQAAMANLYELDAIAAAIIGGTLLSGGRGTIIGALFGVLVFSTITNLFAINNLSTEVQNMVKGGIIVAAVLIQQFRYRSLTQLLGRKPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWD2</accession>
    <name>U5VWD2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14410"/>
    <dbReference type="EnsemblBacteria" id="AGZ41164">
        <property type="protein sequence ID" value="AGZ41164"/>
        <property type="gene ID" value="AFR_14410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2924"/>
    <dbReference type="HOGENOM" id="CLU_102838_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="25238" checksum="CCCA66A1D6497BD5" modified="2014-01-22" version="1">MSDLRTRIEQLGGPIEAASEDAIVADIARGRRAVRRRRTVTAAAGSAFGVAAIVAAVSLAGTGTGPGAGPAAAPVPAVVTNAAGNLKLVAYRGDQSRWFTIDKVPEGFFIQHDYYGGLTLAPEKARNRPAGIKPSTPAGELYDPQILTGKIGIYLELEEYRGELSGETLTVAGKQAILHPLEGGTQQLIIAVTSQVYATIQVDVPLSREQILELGAGLHVHQDAVDRMAAAAGVVPEKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWH8</accession>
    <name>U5VWH8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_14275</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361196.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14275"/>
    <dbReference type="EnsemblBacteria" id="AGZ41137">
        <property type="protein sequence ID" value="AGZ41137"/>
        <property type="gene ID" value="AFR_14275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2897"/>
    <dbReference type="eggNOG" id="COG2409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005108_5_0_11"/>
    <dbReference type="OrthoDB" id="149255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="506"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="536"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="616"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="652"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="190"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="700" mass="72812" checksum="3C02D3FE7F853E00" modified="2014-01-22" version="1">MLAALGRTVHDRRLLVIAVWVVVALAGAVFGGSVYDRTQTIDTLPEGAPATVAQDRLDKVSPEGERLVAVVSGRDVGATDLVNSVTRIAFEIRDIPGVAEVEDSYTTASRKISRDSKSSLVTVELKPGLSDDEALRVADRVSAALRRIDAPEILIGGELLAEREFGDQAVRDAAVGESVALVCVGLLLVVVLGGLVAGLLPLAAALGAVTATLLALTGLARFLPVSDYAVNVVTLLGIGLAVDYSLLMIARFREERAADARAPLVELLARTVATAGRAVLVSGSAVTAALVGLFAFGDPLLAAMAMGGALVVALTTATGLTLVPALIAVAHRRIPARRSRPRRGTDVLARLAAFAQRRPAAVALTATLGLLALGTPLFFVEFANSDVRALPRSSEARQTYEAVQGRFADPPKAPLTIVVEADPAGPGLTQFFDTVRGQPGVSDVFVRSDLPPGVSVADVVPSGTADGEQAQGLVARLRALDAPAPVLVAGPAAELVDAKRSVAGRLPLALAIIVLATGALLFALTRSVVVPVKAIVMNLLTLAATMGVLVAVFQWGWAAGLLGFEPWGALDLTTPLLLFVFAFGLSMDYEVFLLARIKEEWDRRTSTDRAASDRAVLAGITATGPVVTTAALAIGVVFLGFVVGDLVAVKEIGVGMAVAVLLDVTVVRGLLLPATMSLLGRWNWWRPGVTTKQAPRVPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWL5</accession>
    <name>U5VWL5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-xylanase</fullName>
            <ecNumber evidence="6">3.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_14910</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-xylosidic linkages in xylans.</text>
            <dbReference type="EC" id="3.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14910"/>
    <dbReference type="EnsemblBacteria" id="AGZ41264">
        <property type="protein sequence ID" value="AGZ41264"/>
        <property type="gene ID" value="AFR_14910"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14910"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3028"/>
    <dbReference type="eggNOG" id="COG3693">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020161_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031158">
        <property type="entry name" value="GH10_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="PTHR31490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00134">
        <property type="entry name" value="GLHYDRLASE10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00591">
        <property type="entry name" value="GH10_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 6 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665790" description="Beta-xylanase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="GH10" evidence="10">
        <location>
            <begin position="21"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="9">
        <location>
            <begin position="344"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="322"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="331"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ41264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="444" mass="45811" checksum="E3A9EFACF3CBB00C" modified="2014-01-22" version="1">MLSAASAGVVLAASIAVVGTAEAASTLGASAAQTGRYYGAAIAAGRLGDSTYTRILNTEFNSVTPENEMKWDATEPSQGRFTYTNGDRILNQGLSNGSKVRGHALLWHAQQPGWAQALSGSALRNAAINHVTQVATHYKGKIYAWDVVNEAFADGGSGGRRDSNLQRTGNDWIEAAFRAARAADPAAKLCYNDYNTDGINAKSTGIFNMVRDFKSRGVPIDCVGFQSHLGTGIDSTYQANLKRFADLGVDVQITELDIEQGGNQANIYSTVTKACLAVSRCTGITVWGIRDTDSWRTGANPLLFDGSGNKKPAYTAVLNALNAGGTTNPTTPPVTSPPASPTTPPPSGSGCTATVSLNSWSGGYVATVKVTAGSSAVTGWTVSATLPSGGALTGVWSATNTGTTGAVSFRNVEYNGRIPAGGSTEFGFQGTGTGPSAAPACRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWZ7</accession>
    <name>U5VWZ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09755"/>
    <dbReference type="EnsemblBacteria" id="AGZ40240">
        <property type="protein sequence ID" value="AGZ40240"/>
        <property type="gene ID" value="AFR_09755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1989"/>
    <dbReference type="eggNOG" id="ENOG5030F70">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1657093_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="159" mass="17487" checksum="176574F25894DC21" modified="2014-01-22" version="1">MIELGPLAEIGAARARLDERELELIDRARQSGATWGQVATALGLASRQAAEQRRQRLVAAARSRRHSQDLRYAHSIVVLRAAVDDLHRSLTADRRWESRFTRAALVRATVATALDAPPGGLFALAEQAADDLSRLDRNLLPGHVRIALDALQRALSTDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXR7</accession>
    <name>U5VXR7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_16325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361622.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16325"/>
    <dbReference type="EnsemblBacteria" id="AGZ41547">
        <property type="protein sequence ID" value="AGZ41547"/>
        <property type="gene ID" value="AFR_16325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3311"/>
    <dbReference type="eggNOG" id="ENOG5030NUB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_451756_0_0_11"/>
    <dbReference type="OrthoDB" id="1581481at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="604" mass="65964" checksum="B0B81D0FF98E7EF5" modified="2014-01-22" version="1">MFGRRTRPAVPDQSVNRFGNTCPVCGAALGAVDLELDRATGRFKLPPSMRLEVGPADAENRNSWRVTVLSVNASGLPYEPLTSTTPRFIAEEVEYTYLLCGGGHIFPDVVSVFDQAGGGHLRNAQRIDGWNMVAAIGAPASGKTYLLVRMLHQNLDTPDGFDLSSGASGRVRLRQLTPLERMPLAVRAERYKRTVSEGHPIPPTNTEQEGKPVGILDRALPSALEDGIRQLVFKTVVDGQRRAEEWGRNFRQPLVLRTDSQGRRFWTGVADLPGELFNPDDTQPQEAGKLRGFDSLLWVVDPVVAANALDWLVADSLPDVTDYPMMLDGSLRPGASESSGHKVVRGNRDHFQIEIGRQLTLIDGILTRGPEQNLNLFVALSKCDLIHAALRNKKLTDLGAPDVVRRGVARYLFTTARKLAEQRLTADEAATGLLVYLHGGAAGSAVARQERIEHIADGLLRVYSDPTRFWNLVHSGRAEEVVITDGPTMNLQGWHLSVPSLSDHLTAALVPETGHRLLLRDLVMSALGCGLMFGLGHQDAIASLLHDNWLTLKFFLCSPLGTVPMSTSNQLITPLVGGDRFPQAHERSAALTQLMLAVLGKARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY32</accession>
    <name>U5VY32_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative xyloglucan glycosyltransferase 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18150"/>
    <dbReference type="EnsemblBacteria" id="AGZ41908">
        <property type="protein sequence ID" value="AGZ41908"/>
        <property type="gene ID" value="AFR_18150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3684"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020629_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="423" mass="47214" checksum="2E3607553248AC63" modified="2014-01-22" version="1">MAAAVMIVVSLVLAGFAAVTLWWTMHAWRTPETLASTRFAAPDGAHRLTFSLLVPARHEQAVLEHTVGRLLESSHEGFEIIIIVGHDDPETAEVAHRVAARSPGRIFVVTDTNEQKNKPRALNTALPYCRGDVVGVFDAEDQVDPLLLEHVDHAFRSTNADVVQGGVQLINFHSSWYSLHNCLEYYFWFRSRLHLHAAKGFIPLGGNTVFVRTDVLRSAGGWDGNCLAEDCDLGVRLSSQGARVVVAYDSAIVTKEETPGTLTSFLKQRTRWNQGFLQVYRKGDWHRLPAFGQRLLARYTLTQPFLQGFSGIVIPIGIAIAIWARLPVFVAMITFVPVIPWAFNLVFQTVGLRDFGKHYDLRIRWHHYARLLLGAFPFAVVLAVAALRAVWREYTGKRDWELTAHTGAHLDQKHVPAESGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYD3</accession>
    <name>U5VYD3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12170"/>
    <dbReference type="EnsemblBacteria" id="AGZ40720">
        <property type="protein sequence ID" value="AGZ40720"/>
        <property type="gene ID" value="AFR_12170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12170"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_4_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="133" mass="14752" checksum="66B1B55E06D2929E" modified="2014-01-22" version="1">MVDRVVTDELWLRLEPLIPVPERRYRFPGRRRADNRAALEGILFVVSTGMRWSDLPTAMFGASGATCWRRLKEWHQAGVWQQLHQTVLAELRAAGLLDLAHAVVDSSHLRALKGGTSPVPARSTGAGWPASTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYI8</accession>
    <name>U5VYI8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360775.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40716">
        <property type="protein sequence ID" value="AGZ40716"/>
        <property type="gene ID" value="AFR_12150"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12150"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2471"/>
    <dbReference type="eggNOG" id="ENOG5032ZWA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_691929_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021235">
        <property type="entry name" value="DUF2637"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10935">
        <property type="entry name" value="DUF2637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="398" mass="40820" checksum="7A9E5963240000CC" modified="2014-01-22" version="1">MAAGGITALRNLAAGAVAGIAAWSSWYHMSHLARLHGEPAEVAVALPLTVDGMLIVATIVMADDKRRYGVVRPVARIAFPIGVIASVAANIAGADPTLWARFIAAWPPIALLLIVEMLARPPAGSAAVPAGTEQGPRPGEAEPVVPHTAIGEVPPQPQVPPQREVPPAAGGVGVPRLRHPAAYPELVPLPPHVVSAAAGTSAGGAAGHRMPSSNSSREEQLTGGQNVDATQVPVPLTTGSQVHLEVPVAAELAGVEADGEVPSAVGPAGREAGLVAAEPLACSDEAPKKFRSALAPTDRAAADRLHVVADLRTPERPAHRSTGEGGSAPQQPSAQSNSVIAGADDNCGKRTAGDVRRPAATTRQLALTIMQEEPKLTRVEVARRLGVSTRRLREVLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYJ6</accession>
    <name>U5VYJ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-methyltryptophan oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">solA</name>
        <name evidence="4" type="ORF">AFR_18430</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18430"/>
    <dbReference type="EnsemblBacteria" id="AGZ41964">
        <property type="protein sequence ID" value="AGZ41964"/>
        <property type="gene ID" value="AFR_18430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3740"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PTHR10961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="3">
        <location>
            <begin position="5"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="383" mass="41282" checksum="97BFBBFD3EE5268C" modified="2014-01-22" version="1">MPGYDVIVVGLGGMGSAAAYHLAARGVRVLGLEKFGPAHDRGASHGGSRITRQSYFESPEYVPLLLRAYELFDGLARDSGRDVITLTGGVMVGRPESLTVSGSRLSAERWGLEHEMLDAGDLRRRFPTLSPEPDEVALFEAKAGFVRPEATVSAHLELADRAGADLHFDEPMTSWSAAPGGGVRVETAAGSYEAGQLVITPGPWAPQLLSDLGVPFTIERQVQYWFQPTGGVEPFVPERHPIYIWEDAAGSQIYGFPAIDGPDGGAKVAFFRRGIVTTPETIDREVHPDEITAMADRTRRNLPDLPGTFLTAKTCLYSNTPDEHFVIARHPAHEAVTVACGFSGHGFKFVPVVGEILADLATTGTTQHPIGLFDPRRLSGVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ11</accession>
    <name>U5VZ11_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <shortName evidence="5">G6PD</shortName>
            <ecNumber evidence="5">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">zwf</name>
        <name evidence="9" type="ORF">AFR_19085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362462.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19085"/>
    <dbReference type="EnsemblBacteria" id="AGZ42089">
        <property type="protein sequence ID" value="AGZ42089"/>
        <property type="gene ID" value="AFR_19085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3875"/>
    <dbReference type="eggNOG" id="COG0364">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013524_5_0_11"/>
    <dbReference type="OrthoDB" id="1153269at2"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="PTHR23429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G6PD_N" evidence="7">
        <location>
            <begin position="9"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="G6PD_C" evidence="8">
        <location>
            <begin position="188"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="464" mass="51164" checksum="C85BC33151C70548" modified="2014-01-22" version="1">MATKKTVFVLFGATGDLARRMVLPAFYTLAIEGLLPEDWVLVGNGRGDVAHEDFRKHVHDVLVEFGPHPDRGPWKEFEQRLYFAGGGFDTDSPGSLLDVIGEARRRVGARAQLVHYLAVPPVTFPELTKALGQHGLAKGARVVYEKPFGTSPKGFRSLNRIVHSVLDESQVYRIDHFLGKEATQNLHVLRFANELFAAMWNRDHIESVQIDVPEDLGVDDRAGFYDATGAVLDMLVTHLFQVAAEVAMEPPETLTAPDLQSARERVIRAFRPLDPAEVVLGQFDGYRKVKGIEPRSTTDTYVAAKLWIDNPRWQGVPFLLRTGKRMAVSEQRVTVVLRTPQGPLKGLPGQANALSFSLAGNGEIALSLLAKKPGIELHLESATVKLPLAELPDAEPLPPYVRLIHDVLLGDRSLFTRPDGLSAAWRTVQPILDNPPRLHKYAQDSMGPAAAKKLAAPHGWLLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ16</accession>
    <name>U5VZ16_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative FAD-dependent monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18730</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362395.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18730"/>
    <dbReference type="EnsemblBacteria" id="AGZ42022">
        <property type="protein sequence ID" value="AGZ42022"/>
        <property type="gene ID" value="AFR_18730"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18730"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3803"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032067_2_0_11"/>
    <dbReference type="OrthoDB" id="324612at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="475" mass="52770" checksum="E9A9DC833ABF4D17" modified="2014-01-22" version="1">MAEEFDVLIIGAGLSGIGAAWRLRHERPGTTFAVLEARDAIGGTWDLFRYPGVRSDSDMFTLSYPFRPWRGVESLAPAEAIRNYIQDTADEGGITPHIRFGSRVETASWSGGRWTVRLTDGRTLTCRFLYACAGYYDYARGHQPDFAGLDDFTGRFVHPQSWPEDLDHTGKRVVVIGSGATAVTLVPAMAPAAAHVTMLQRSPSYLTVLPGRDAVADALRRVLPAKAAHRVARAKNIVLSQAFYQLARRRPDRVKALLRRFALRYLDDPAYVDEHFTPAYQPWDQRLCVVPGGDLFEAIRGGKASVVTDHIERFVPEGIRLRSGRTLEADIVVSATGLSLLPIGGVTLTVDGQVVDPGTRVAYRGLMLSGVPNFAYCIGYTNASWTLRADLSHRYVLRLLSYLDKHGYAAATPKETPGNRRPLLDLTSGYVQRALDRFPQQGDKDPWTVRQNYLRDVVTTPRADVSRDMSFEESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W051</accession>
    <name>U5W051_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362950.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21540"/>
    <dbReference type="EnsemblBacteria" id="AGZ42578">
        <property type="protein sequence ID" value="AGZ42578"/>
        <property type="gene ID" value="AFR_21540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4368"/>
    <dbReference type="eggNOG" id="COG4427">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065141_0_0_11"/>
    <dbReference type="OrthoDB" id="1560248at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011200">
        <property type="entry name" value="UCP012608"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10094">
        <property type="entry name" value="DUF2332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="33122" checksum="B63B1926D70A7FA4" modified="2014-01-22" version="1">MTYADFGTREARGVSPTYERLALAVSRDDELLALIATLPPAKRQPNLLFGVVRFLGGPVEDPAVFHDWVLEHWPAIEPELLSRATQTNEAGRCAVLLPVLAALPGPLALLEVGASAGLCLYPDRYSYRYGDHTLGTGEPLLECDATGLTPPAQLPQVVWRAGLDLNPLDVTDPADVAWLDALIWPEHHHRRSRLRAAAAVAAADPPHLVRGDVLDDLPALAAQAPPDATLVVFHTSVLYQVPAPRREAFAELVTSLPGHWISNEAPDVVALDNLPDPPDASLHNVLALDGTPLAWTRGHGQSLTWFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W080</accession>
    <name>U5W080_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lactone hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361385.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15220"/>
    <dbReference type="EnsemblBacteria" id="AGZ41326">
        <property type="protein sequence ID" value="AGZ41326"/>
        <property type="gene ID" value="AFR_15220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3090"/>
    <dbReference type="eggNOG" id="COG3386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036110_4_0_11"/>
    <dbReference type="OrthoDB" id="852341at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGL" evidence="1">
        <location>
            <begin position="15"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="298" mass="31768" checksum="DDECCC23DF25D871" modified="2014-01-22" version="1">MTQIKTVADGFHFLEAPRWREGRLWFSDFYGLQVHSMLPDGSDRRVEADVPGQPAGLGWLPDGRLLVVSMRDRKLLRREEDGTLVVHGDLGNHATGHTNDMVVDAQGRAYVGNFGFDLMGGAPVGTAGLHRVDPDGTVTQVADDLWFPNGSAITAGNVLVVAETFGNRVTAFDLTSEGELVNRRPWATFGPLPQSRNLGDTLAELSLAPDGVCLDADGGLWIADALGSRLVRVLDDGTITDEIEPGTPVYACALGGDDGATLFACAAPDFHESARKNAHEGHMIAIRLTGQGRPSAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W092</accession>
    <name>U5W092_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Apolipoprotein N-acyltransferase</fullName>
            <shortName evidence="8">ALP N-acyltransferase</shortName>
            <ecNumber evidence="8">2.3.1.269</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">lnt</name>
        <name evidence="10" type="ORF">AFR_21790</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the phospholipid dependent N-acylation of the N-terminal cysteine of apolipoprotein, the last step in lipoprotein maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a glycerophospholipid + N-terminal S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein] = a 2-acyl-sn-glycero-3-phospholipid + H(+) + N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48228"/>
            <dbReference type="Rhea" id="RHEA-COMP:14681"/>
            <dbReference type="Rhea" id="RHEA-COMP:14684"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136912"/>
            <dbReference type="ChEBI" id="CHEBI:140656"/>
            <dbReference type="ChEBI" id="CHEBI:140657"/>
            <dbReference type="ChEBI" id="CHEBI:140660"/>
            <dbReference type="EC" id="2.3.1.269"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Protein modification; lipoprotein biosynthesis (N-acyl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CN hydrolase family. Apolipoprotein N-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.269" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21790"/>
    <dbReference type="EnsemblBacteria" id="AGZ42628">
        <property type="protein sequence ID" value="AGZ42628"/>
        <property type="gene ID" value="AFR_21790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4415"/>
    <dbReference type="eggNOG" id="COG0815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019563_0_1_11"/>
    <dbReference type="UniPathway" id="UPA00666"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07571">
        <property type="entry name" value="ALP_N-acyl_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01148">
        <property type="entry name" value="Lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004563">
        <property type="entry name" value="Apolipo_AcylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045378">
        <property type="entry name" value="LNT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20154">
        <property type="entry name" value="LNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00546">
        <property type="entry name" value="lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="207"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ42628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="504" mass="53127" checksum="82241D89A4BA1F94" modified="2014-01-22" version="1">MLLALAAGAALLLSFPPYGLWFLGPVAVALLAAATHRRRLRGGFGVGILAGFVFFVPLLDWTNLAAGWLPWILLCTAQAAYLGLLGVAGAWLSPLHDRWRAVWPLTAGLLWVAQEALRDRAPFGGFPWGRLAFSQGDAPGLRLAVVGGAPLVTFAVAAAGGALIALAWRRWTRPRSWAGAIGFAVAAGVIVAGPALMPVAKPAAATTTVAIVQGNVPRLGLDFNAQRAAVLLNHVRATENLAEEVRLGRQPKPELVIWPENSSDIDPLRNPDAAQQIAAAATAIGVPILVGAVLRSDVPGEIRNAGILWQPVTGPDLDQLYVKRHPVPFAEYMPLRPIARVVSDKVDLVKNMVAGDTPGVVRTPAATIGDVICFEVAYDSIVRDTVTGGAQILAVQTNNATFNESEARQQLAMVQLRAVEHGREALMVSTVGVSAFVGTAGQVYSETGFNTRAVVLHDMHVGGPRTLATRLGHWPEFAAVGLAVLLLAGAAPLRRRRNGGSPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0I0</accession>
    <name>U5W0I0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_22290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22290"/>
    <dbReference type="EnsemblBacteria" id="AGZ42728">
        <property type="protein sequence ID" value="AGZ42728"/>
        <property type="gene ID" value="AFR_22290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4519"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_40_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="TetR_C_33" evidence="5">
        <location>
            <begin position="74"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="197" mass="21371" checksum="A6533A331DDF7CE5" modified="2014-01-22" version="1">MRTLLLERAAQMLRAREPITLRSLVAGTGVSTMAVYTWFGDMDGVWKALRQEGFTRLAARFAAFTPPADPVEELAGLVAAYVGNALDHPDLYRVMFDATVDLEDLPAADATLEHLVRAAERGRVAGRFRADLEPLDLAIRSWVIGHGLVSLVTPGPLPPETLTHAVPMLTALFIGTGDKPEKCRRSVERGWAGGQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0K2</accession>
    <name>U5W0K2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21795"/>
    <dbReference type="EnsemblBacteria" id="AGZ42629">
        <property type="protein sequence ID" value="AGZ42629"/>
        <property type="gene ID" value="AFR_21795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4416"/>
    <dbReference type="eggNOG" id="COG0145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046680_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006230">
        <property type="entry name" value="MutL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13941">
        <property type="entry name" value="MutL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="415" mass="41522" checksum="3ABF551AEEB5381E" modified="2014-01-22" version="1">MDVGSTYTKACLVDLDGGVLLRRAEVPTTAGTDVLTGLDAAVAQLGGTGELYVCSSAGGGLRLAVVGYEALVTAEAGHRVGLSAGAQVVHVAAGALDGRAVAALRASRPDVILLVGGTDGGDGEVLLHNARRLGTSRMRVPVVVAGNADVRDEAAEVLTGRRIPVTVTGNVLPRIGVLDPGPARAAIREVFLRHVIGGKRLSRGGRFGSLVRAATPDAVLAGVELLADVTGAGVLVVDVGGATTDVYSALLPDAESETGPRRDVAGTLWRSRTVEGDLGVGVGAPGVVAAAAAEKLPVPDISGGPPYSAGTDRRLAATAATIALRRHARGYSAGPGQPRTGGRDLRDVRLVVGSGGVLRHGGGEEVLDAVLGDTAGGWAAPAAPRRTVDAQYVLAAAGLIAVDHPAAAAGLVHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Y1</accession>
    <name>U5W0Y1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">R2K_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361694.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16660"/>
    <dbReference type="EnsemblBacteria" id="AGZ41610">
        <property type="protein sequence ID" value="AGZ41610"/>
        <property type="gene ID" value="AFR_16660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3378"/>
    <dbReference type="eggNOG" id="ENOG502Z879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040789_0_0_11"/>
    <dbReference type="OrthoDB" id="845510at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025643">
        <property type="entry name" value="R2K_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14243">
        <property type="entry name" value="R2K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R2K_3" evidence="1">
        <location>
            <begin position="132"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="30704" checksum="F7A7CE9509E08916" modified="2014-01-22" version="1">MILLVPADALRPRRPDEHFAPEAAAARESGLDVAVIDHDALARGDDPAAAVARVPSGGTAVYRGWMLHSPQYAALASALAARDVVLRTSADQYRRAHELPGWYDALAAVTPESAWTTGDDRDAFEHACDTLRSGAAVLRDYTKSMKHYWHEAAYIPDISDREAAWRVASRFRELRGDDFTGGFVLRRFETFTGGAEARTWWVDGVCRLVTAHPDTPDTVPPADLHEVTPLVTALGLPFVTVDLAQRDDGRWRVIELGDAQVSDRPTTTSPGELIAAAVHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W144</accession>
    <name>U5W144_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Citrate synthase (unknown stereospecificity)</fullName>
            <ecNumber evidence="3">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_16665</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361696.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16665"/>
    <dbReference type="EnsemblBacteria" id="AGZ41611">
        <property type="protein sequence ID" value="AGZ41611"/>
        <property type="gene ID" value="AFR_16665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3379"/>
    <dbReference type="eggNOG" id="COG0372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025068_2_1_11"/>
    <dbReference type="OrthoDB" id="1412360at2"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="PTHR11739"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="370" mass="39943" checksum="97D8D53A7F2AB40B" modified="2014-01-22" version="1">MLTVKPGLADVVAFDTQIAEPDRSGGSLRYRGINIETLIGRASFADVWGLLVDGDFRRALPAAEPFAVPVHSGDIRVDVQAATAMLTPYWGLRPLIDIDEAQAREDLARTSVMVLSFVAQAARGVGRPAVPQRRVDEGRTATERFMIRWQGDPDPRHVEAIDRYWVCAAEHGMNASTFTGRVVASTGTDVAACLSAAVASLSGPLHGGAPARALGMVEAVERGGDARAYVKQVLDSGRRLMGFGHPVYRAEDPRARILRAAARELGAPRYEVALALENAALAELRERKPDRVLETNVEFWAAVVLDFAGVPAELFTPLFTCARTAGWSAHIIEQKRLGKIIRPSADYVGPAGRSPESLPGWDRLSVTMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1L7</accession>
    <name>U5W1L7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydroxyacetone kinase subunit DhaK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363446.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23235"/>
    <dbReference type="EnsemblBacteria" id="AGZ42917">
        <property type="protein sequence ID" value="AGZ42917"/>
        <property type="gene ID" value="AFR_23235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4709"/>
    <dbReference type="eggNOG" id="COG2376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017054_0_0_11"/>
    <dbReference type="OrthoDB" id="857242at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012736">
        <property type="entry name" value="DhaK_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004006">
        <property type="entry name" value="DhaK_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02733">
        <property type="entry name" value="Dak1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02363">
        <property type="entry name" value="dhaK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51481">
        <property type="entry name" value="DHAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaK" evidence="1">
        <location>
            <begin position="7"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="35483" checksum="CB7F80B9B2C0B369" modified="2014-01-22" version="1">MKKFVNDPKNYVAEMLEGVALANPGTLKYVPAYNLIMRTDAPREDKVSIVQGSGSGHEPAHVMAVGRGMLDAACPGDVFAAPPMDYVYETAKLLASPKGVLLLVNNYTGDRMAFDMGKEMAEADGVRAEILTINDDVAVQDSTYTVGRRGVAGNFFVIKAVGAASERGAEMDEVLRIGRKVNDVTRTMGVALTACTPPAKGSPLFELGADEIEFGVGIHGEPGRERRKIKEADAIVDDLLEAVTGDLPYTSGDRVALMINGLGGTPISELYILYRHAHQQLAARGLTVGRSYVNEYCTSLDMAGASLTLVRLDDEIEDLLAARAEAAVRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1V8</accession>
    <name>U5W1V8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362284.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18165"/>
    <dbReference type="EnsemblBacteria" id="AGZ41911">
        <property type="protein sequence ID" value="AGZ41911"/>
        <property type="gene ID" value="AFR_18165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3687"/>
    <dbReference type="eggNOG" id="ENOG5033N66">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1575129_0_0_11"/>
    <dbReference type="OrthoDB" id="2098446at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665455" evidence="2">
        <location>
            <begin position="27"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="17660" checksum="1DCE69C5225B9679" modified="2014-01-22" version="1">MTGKRRLAIIAGVLGVVAAGTWFVVAAGESETSPPAASAPRPTDSTWPLLDSPGATAPVTSSAAAPTTRSTRAVPPPKKAEPFVQSFAGQPGVRPQKPLPSPTSTHYVRPNVDGCDHNYGTITQCVPWTFPAGTKDKCAWLADHGFKKLAVAGKDRQKLDTDGNKIACD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Y0</accession>
    <name>U5W1Y0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363699.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24205"/>
    <dbReference type="EnsemblBacteria" id="AGZ43109">
        <property type="protein sequence ID" value="AGZ43109"/>
        <property type="gene ID" value="AFR_24205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4903"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="26024" checksum="12F64931CADBD93D" modified="2014-01-22" version="1">MNHDTIQVRAVTRTYGSGDGAVTALDRLSLSFAAGTFTAVMGPSGSGKSTLLQCAAGLDRPTSGSVLLGGTELTALGETRLTRLRRERIGFVFQAFNLLPALTAEQNVALPLRLAGRRPARAEIRAALTRVGLGDRARHRPDELSGGQQQRVALARALITRPEVLFADEPTGALDSTTGREVLRLLRGMADDGQTVVMVTHDPVAAASADRVVFLVDGRLHGELSTPSAASVADRMTRLETAPCSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Y9</accession>
    <name>U5W1Y9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA ligase D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24255"/>
    <dbReference type="EnsemblBacteria" id="AGZ43119">
        <property type="protein sequence ID" value="AGZ43119"/>
        <property type="gene ID" value="AFR_24255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4913"/>
    <dbReference type="eggNOG" id="COG3285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008325_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="424" mass="46442" checksum="89430906BF13C4B8" modified="2014-01-22" version="1">MPGVRADLARLIRIPGIAFDGFDHTHVERSAAAVAELAAAADCRPTSSARVGNRRSSGTGPHHPARTGRTRACTWPSSPGPASPRHFFSSGWAFRGPPAGYPARMPITDPLTPVEAGELDSLGAGGSWTVFGRQLRVTNLDKVLFPGRDDRPPVTKRDFLRYTARIAPTLTPYLAGRPLNLHRYPNGAQTKGFWHKELPAHAPAWLSRWDNPEADPGETRTYLVADEPAALVWAANFGALEWHPWTSRAEAPHRPTYALVDLDPGPATGWEDVLTLARLHRTAFEHLGVTARAKVTGRRGIQIWVPIVNGPGFEETRAWVQELSRTVGAVVPELVSWKWQKSDRGGLARLDYTQNAINRTLVAPYSPRPAPGAPVSAPIGWDELDDSALRPDGFTIDDVLERLAERGDLFHAVLDRPQVLPPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W243</accession>
    <name>U5W243_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363784.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24640"/>
    <dbReference type="EnsemblBacteria" id="AGZ43194">
        <property type="protein sequence ID" value="AGZ43194"/>
        <property type="gene ID" value="AFR_24640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4992"/>
    <dbReference type="eggNOG" id="COG4430">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076645_1_1_11"/>
    <dbReference type="OrthoDB" id="1966598at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="22037" checksum="885D9BBC86A92FCF" modified="2014-01-22" version="1">MGAAELDELIVADAEALRAWLSDNHATSPGVWLALTRKGGLVTTVTWQHAVDEGLCFGWIDGQGRKGNQDISWIRFTPRRARSSWSQRNVSHVARLEEQGRMHPSGRAVVEAAKADGRWAAAYAPPSEAEVPADLLAAIAAVPAAQAMLDVLTKTNKFALIHRVNAVKRAETRERKIGEFVAMLARHETFYPQKAKPPTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W259</accession>
    <name>U5W259_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24095"/>
    <dbReference type="EnsemblBacteria" id="AGZ43087">
        <property type="protein sequence ID" value="AGZ43087"/>
        <property type="gene ID" value="AFR_24095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4881"/>
    <dbReference type="eggNOG" id="COG2890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067079_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006764">
        <property type="entry name" value="SAM_dep_MeTrfase_SAV2177_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04672">
        <property type="entry name" value="Methyltransf_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017393">
        <property type="entry name" value="MTase_SAV2177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28989" checksum="16CBBDF5FF22207B" modified="2014-01-22" version="1">MDVADGQQEMPKLDTTVPQTARIWNHLLGGKDNFAVDRAVGDQIIATLPQLAENARLSRAYLARVVRHLAGTAGVRQFLDIGTGLPTADNTHEVAQKVAPESRIVYVDNDPLVLAHARALLTTSPEGATDYIDADLRDAGTILREAARTLDFDKPVALFLMGILGHIEDDAEARTIINAIMDRLPSGSYLAMYDGSDTSEANNEAVRIWNISANPKYHLRSAARIASLFEGLELVDPGVISVTRWKPEPGAEDAPEIDQYGAVGRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W270</accession>
    <name>U5W270_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362428.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18905"/>
    <dbReference type="EnsemblBacteria" id="AGZ42055">
        <property type="protein sequence ID" value="AGZ42055"/>
        <property type="gene ID" value="AFR_18905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3840"/>
    <dbReference type="eggNOG" id="ENOG502ZFA9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093382_0_0_11"/>
    <dbReference type="OrthoDB" id="1598505at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="253" mass="26777" checksum="D30E107777A5211E" modified="2014-01-22" version="1">MRQQDERDLRTVLREEAERHRPDRDAMRDRIARGRAEETRRPMDRVLSLMRPLAAAAAVAVVLGLGIAGVRLSDQGGIDDSTTPVAAPTTFTPATPAPTKATPAAPRASASSGTTTRTQAAHPGKDGFLSSSGRLDPHSNDGWSQDNVVLTTTRTITELKVSVHVALTAGVVETGRWSTVPANLLSMTSSRKDDTLVFRFTLAKGAKLGPGSYTFAVQFNHAAGKRSPAGDTYSAEAAAGDKDVQVTGTVTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W272</accession>
    <name>U5W272_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-binding monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25335"/>
    <dbReference type="EnsemblBacteria" id="AGZ43333">
        <property type="protein sequence ID" value="AGZ43333"/>
        <property type="gene ID" value="AFR_25335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5134"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_14_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="2"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="568" mass="61554" checksum="0BEA65C99A788CBE" modified="2014-01-22" version="1">METDVLIVGSGPAGSAAAMLLSTYGVPNIMVTKYSRLADTPRAHITNQRTMEVLRDAGLEDVVVAQATPQHLMGQTPFCSSIAATEELGRLHTWYTHPNRQADHDLASPTAICDMPQHLMEPVLASAATARGTHLRYDTEYLSLTQDDDGVTATVRDRLRGDEYQIRAKYLIGADGGRSRVAEDIGLPTVGRMGVAGSINVVFEADLSRYVAHRPSTLYWVLQTGADVGGIGMGLVRCVRPWHEWLIVWGYDIEGEPPDLTEEYARRLAHQLIGDDTVPVTIKSSSAWTVNHLYAERYHQGRVFCAGDAVHRHPPSNGLGSNTSIQDAYNLAWKLELVLSGAAGPALLDSYSAERAPVGRQIVERANRSIGETGRIFQALEGGLDARKLPGEGAEKQRQLLREAVAFKDYEFNTHGVELDQRYVSDAVVPDGTPAPRNPRDAELYHHATTWPGAKVPHAWLGRGTERVSTLDVGGRGRFTLLTGVGGEAWAAAAADVAEELGIELTAATIGPGADYEDLYGDWAALREVGDTGAVLLRPDNYVCFRSPALAADPAEALRTALRQVLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W384</accession>
    <name>U5W384_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_20940</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20940"/>
    <dbReference type="EnsemblBacteria" id="AGZ42460">
        <property type="protein sequence ID" value="AGZ42460"/>
        <property type="gene ID" value="AFR_20940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4245"/>
    <dbReference type="eggNOG" id="COG4132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_18_6_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="81"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="32139" checksum="3BC4001890D3662E" modified="2014-01-22" version="1">MATVTDSPGLLAPVPEVRAPRRKFSAGRLSSLLGTVPFFTYVVLFLIIPTLVVVIGAFAGDGGFTLSNISALGDEYILDAFGRSLLLSAVTAVVGAVLGAVLAYALATAKPGGLLRRMVTAASGVLAQFGGVTLAFAFVATIGLSGFVTVFLRDHVGVDLYANGVWLFELPGLVLVYTYFQVPLMVIVFLPALDGIRPQWREATESLGGSTWQYWTRVAGPLLAPAFLGSTLLLFANAFSAYATAAALVSQGNPIVPLQIRSALTSEVVLGQQNLGKAMALGMVVVVAVVMTLYALLQKRTAKWLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3A9</accession>
    <name>U5W3A9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27160"/>
    <dbReference type="EnsemblBacteria" id="AGZ43693">
        <property type="protein sequence ID" value="AGZ43693"/>
        <property type="gene ID" value="AFR_27160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5504"/>
    <dbReference type="eggNOG" id="COG2183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009833_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05685">
        <property type="entry name" value="S1_Tex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044146">
        <property type="entry name" value="S1_Tex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018974">
        <property type="entry name" value="Tex-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032639">
        <property type="entry name" value="Tex_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09371">
        <property type="entry name" value="Tex_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16921">
        <property type="entry name" value="Tex_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="661"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="848" mass="90057" checksum="B77F9734FFEECA38" modified="2014-01-22" version="1">MSDPSVTIVGVTTTIHQRIAQELGVREGQVTAAVDLLDGGATVPFIARYRKEVTGTLDDQQLRTLEERLGYLRELEDRRAAVLESIRSQGKLDDALEAQILGAESKARLEDIYLPFKPKRRTRAQIAREAGLEPLADLLIGDATRDPKAEAAPYVDADKGVADVAAALDGARAILIERFAEDPDLIGELRERMWTKGRLVAKVRDGKQTDGAKFADYFDFAEPFTKLPSHRILAMFRGEKEDVLDLTMEPEEGVEDGTPSSFEASVAGKFAITDHGRPGDRWLLDTVRWAWRTRILIHLGADIRVRLWQAAEDEAVRVFAANLRDLLLAAPAGTRSTMGLDPGFRTGVKVAVVDATGKCVATSVIYPHVPQNKWDQSVHTLAALAKEHNVDLVAIGNGTASRETDRLAADLIKLHPDLGLTKVMVSEAGASVYSASAYASQELPGMDVSLRGAVSIARRLQDPLAELVKIDPRSIGVGQYQHDLSEVKLSKSLDAVVEDCVNAVGVDVNTASAPLLTRVSGIGAGLAENIVLHRDSNGPFKNRSEIKKVARLGPKAFEQCAGFLRIPDGDDPLDASSVHPESYPVVRTILATAGGDLKTVIGNSPLLRALRPEKFVTDTVGLPTVTDILRELEKPGRDPRPAFTTATFADGVEKIADLRPGMILEGVVTNVAAFGAFVDVGVHQDGLVHVSALSNTYVKDPREVVKSGDVVKVRVVEVDEVRKRISLTMKLEDKPEQRAPREPREPGGQRQGGGGGQQRRGGGGPQGGQAGGGGKGGGQGGGQSGGQGGGRGQGGGQGGGGQRQGGGQPRQGGGQRQGGGRGQGSSSGGGFGNDAMADALRRAGLTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3F7</accession>
    <name>U5W3F7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20995</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362843.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20995"/>
    <dbReference type="EnsemblBacteria" id="AGZ42471">
        <property type="protein sequence ID" value="AGZ42471"/>
        <property type="gene ID" value="AFR_20995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4256"/>
    <dbReference type="eggNOG" id="ENOG5034717">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2142983_0_0_11"/>
    <dbReference type="OrthoDB" id="1923689at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="113" mass="12261" checksum="C74E99B6909E7516" modified="2014-01-22" version="1">MSVGAHHHHILDPSGQGTVVLNIGQGIGALVLYTPGSLHGHEIEVSPVEDAARRTHAAVRARYVRDGVLFSMVLDNLAEGRYVIWQDPVTPLAEVDIRSGMVTEHEWPVPAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Q3</accession>
    <name>U5W4Q3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bacteriophage (PhiC31) resistance protein PglZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560515.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29585"/>
    <dbReference type="EnsemblBacteria" id="AGZ44178">
        <property type="protein sequence ID" value="AGZ44178"/>
        <property type="gene ID" value="AFR_29585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5997"/>
    <dbReference type="eggNOG" id="COG1524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_330036_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013973">
        <property type="entry name" value="PglZ_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08665">
        <property type="entry name" value="PglZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PglZ" evidence="2">
        <location>
            <begin position="488"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="755"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="908" mass="97785" checksum="4CA6108FC3B2ADE9" modified="2014-01-22" version="1">MTQASVPARALRISPVALRQKVAQQLERHRNGSAHPVMLLRAEPVWPHDPIILLPDGRTAHVVACESPLAIWDQLVADRPDEVLVLLADLAESALGDGVRSRVFRHRVIAVEPWDLVVDAFGARQPDSALEQEAWAGEALVDAMPPAGWPKLATPVLTRDVALRHLAAVRLGLDRRGGGPDDLDVEALLRWSAEPGAVEAFGLLRAAERDGLTKWLTSQYGSPARALFALVAAGHGDEALPLGLVCHAVWRTSDTDAIRARGRIDQYFGKLDDDTTIIGFADAVVQVVHGMLSEPEARRHGHAVLDRAEELLIQFGAVSSAADSPILRTGFTRRIGAAANALLAAMRNPAEQALDAAVRSLTEHELAASELERVRRIEMAQRLVRWLNGDVQPPASVADGVDRQIADWGWVDVAIDHVWAGDDAHPELQAAFREIYGRAQERRRALDEAFAGRLQAWATAGPGNDGGLLTVENLLPRVVEPLLRTDRRVLLVVLDGMSAAVAIQLANELSQHWVEYDPLAGSEAGRRRGVVAALPTLTAVSRTSLFAGALRSGTQDNEKRFFAGGRWGKDAKIFHKGPARGGAGEMFAGELADAVADSGSLVAVVINTVDESLAQGREGSEAGWQLGDIGFLRSLLDLARSSGRAVIITSDHGHVLERGGEKVRAADVASARHRTGAGPAESGEVELSGPRVVAPDNRIIALWDPLLRYQSSKAGYHGGASLAEVTIPLLAFLLPHVTDVPAGWSAVEVRRPQWWQSQSAPTATAAELPSPAPKSRRRPTVAAGETLFDIPEPSAAPTTRDDRADLVDELKESELFAAQHALTPRRLDIKKVEAALRALVEAKGVLPAAVLAERAGEIPARATGFVSTLQRIFNVDNYPVLTLIDGGRTVRLDIRLLREQFRLSGETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5B3</accession>
    <name>U5W5B3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363686.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24140"/>
    <dbReference type="EnsemblBacteria" id="AGZ43096">
        <property type="protein sequence ID" value="AGZ43096"/>
        <property type="gene ID" value="AFR_24140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4890"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055817_1_1_11"/>
    <dbReference type="OrthoDB" id="1060221at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043917">
        <property type="entry name" value="DUF5753"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19054">
        <property type="entry name" value="DUF5753"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5753" evidence="1">
        <location>
            <begin position="101"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="32082" checksum="10BCFA5E75233BBE" modified="2014-01-22" version="1">MPTDEGPTLRRRRLGAELKRCREVAGLTQEIVGRHFEWHAAKVTRIETARVAVTARDVKDLLTLYGVDDEEYREALMALARLSRERTWWTDYRDIMRPGNFVGLEAEATLLRTWEPVLIPGLLQTEAYMRALMRTGRASDPPHHIDRRVALRLKRQGRLTAANPLALAAIIDESVVRRIVGGPEVMKDQLRHLIEVAQLPTVTVQILPFDAGEHPFLGGSAALLEFRETTHLDVVYLEGLAGDYYEEQHSEVARYRDEFERLSAKALDSRLSIKMVEGLLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5D8</accession>
    <name>U5W5D8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30840"/>
    <dbReference type="EnsemblBacteria" id="AGZ44428">
        <property type="protein sequence ID" value="AGZ44428"/>
        <property type="gene ID" value="AFR_30840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6243"/>
    <dbReference type="eggNOG" id="ENOG5033RFZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848964_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="36267" checksum="EDB9D8983FEAA7A4" modified="2014-01-22" version="1">MSSLDEETDLARVADSLRNDVRRKIGALRTQIYEEIHRVGRVAEQRSCQLAARIDTTDERLGGIAGAQDDLRRQTGDEMRGTRQAIARLTGEIHLIEGRLRLEHGVVPVDLDEIPAGLAPLVAQVREAEDIRSSLLDDKTRQDHEQTVSAYAELESAVAESRTRALEASRTLATAKAGGRAFRKAAVVYRRERALLRARTEELRTTRVDRDASQAELAQDVRRRQSYRSHRGATAIDELTDHLRDRIDAAVAAHALFPAWFTITELGHRPPAARAALWREVAVEVLLYRLTHQVRHGVLALGPPPGSGDPLAERHAAVLASLARLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5G7</accession>
    <name>U5W5G7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative HxlR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560788.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30965"/>
    <dbReference type="EnsemblBacteria" id="AGZ44453">
        <property type="protein sequence ID" value="AGZ44453"/>
        <property type="gene ID" value="AFR_30965"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30965"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6268"/>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_0_1_11"/>
    <dbReference type="OrthoDB" id="1919329at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="18334" checksum="F5A221778BB8C53E" modified="2014-01-22" version="1">MNPTISRPPALDWSIETCTIGRAMAVLGEKWTVLVLREIFNGVRRFADMREHTGIPRQVLTNRLSSLVEHGLLRREPYQEPGARIRHEYRLTAKGLDLYPVLTAVKEWGDRYLADPEGPPLEMAHRDCGAEIHTELHCADGHHVADYRQVVPRVGPGARRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5I7</accession>
    <name>U5W5I7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30060"/>
    <dbReference type="EnsemblBacteria" id="AGZ44272">
        <property type="protein sequence ID" value="AGZ44272"/>
        <property type="gene ID" value="AFR_30060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6089"/>
    <dbReference type="HOGENOM" id="CLU_1567363_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="17118" checksum="8F33D4CDA6FEF3AD" modified="2014-01-22" version="1">MLLLLDVMCAAGVLFSAVLIGGLLAGTRLLPTAVAIIVTMVLVLPGFTAGVITNRRTVRGRQPQSGWVRSVWSPPELPRWGIVVTGLVFLAFWAAGMSAFTGLSQDDLPPGDTAAQERQVSHQQRFGLGVLGGIGTGGTALAAGALVRNRRSVRTGERQMAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6M9</accession>
    <name>U5W6M9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative LysE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_26545</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26545"/>
    <dbReference type="EnsemblBacteria" id="AGZ43571">
        <property type="protein sequence ID" value="AGZ43571"/>
        <property type="gene ID" value="AFR_26545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5381"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006324">
        <property type="entry name" value="LeuE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="201" mass="21310" checksum="16D81E3ABF0EF97A" modified="2014-01-22" version="1">MTTFSSLGSFVVVVGLLTLTPGLDTALIVRTAAVGARRRAWGVVLGIQTGTLVWGVLTSAGVTALLTASQVAYEILRWAGVCYLIWMGLSMLRGRAQPEPEATGGGWRRGLLTNLLNPKMGVFYVALLPQFIPAGAPVFAFGVLLTGVHIALGTAWSGVLVLLARRLRVTLQRPAARRVLDRITGTVIAGFGIRLAVQDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7D6</accession>
    <name>U5W7D6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MFS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_28245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28245"/>
    <dbReference type="EnsemblBacteria" id="AGZ43910">
        <property type="protein sequence ID" value="AGZ43910"/>
        <property type="gene ID" value="AFR_28245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5725"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318645_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="208" mass="21147" checksum="A95EDB980BC59F56" modified="2014-01-22" version="1">MAGQRRSSAPLIRLGILRSAALLRANVGALLLVGSFTGFQFAVVLHLQEQRGWSEVETGLALAVIGIDAVLAPTLTPVLVQRFGTPKVVLGGTVLAVAAYASFLSLVADRAFAAMLPSFLLLGLAFALAYGPLTIAATEGVAEEEQGLASGILTMSFQFGAAIGLAVVTAVLVAAGIRAALVVPVVAAVLGVGVALLTVARSSVRTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7N9</accession>
    <name>U5W7N9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN_Toprim_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561359.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33820"/>
    <dbReference type="EnsemblBacteria" id="AGZ45022">
        <property type="protein sequence ID" value="AGZ45022"/>
        <property type="gene ID" value="AFR_33820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6846"/>
    <dbReference type="eggNOG" id="ENOG502ZAR4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049418_0_0_11"/>
    <dbReference type="OrthoDB" id="1452613at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041487">
        <property type="entry name" value="HEPN/Toprim-NTD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18871">
        <property type="entry name" value="HEPN_Toprim_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN_Toprim_N" evidence="1">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="423" mass="45741" checksum="B32FEED7B43EBC4D" modified="2014-01-22" version="1">MGSYGYLKVDSLVLGTYKNGVPAELLAIFRNDMFGTRQVSRTDYFDDADEDEGVLDVVEFRAPAAVIADRLDVMGFEPAAALTLLEEALDRHKLLDTDPGMDVRSEYSRAWSEALRPLTADRWVTQLAAAVPGTGSAHEQGRVISSAAWLLDLLDEEYDPDGRFLLRLVLLARPAGEVILDVTDLAGGGWVPERPAELPSAAMSDMRGVAATHAPVVVLTEGKTDAEMLSGALAVLYPHLTDLVRFLDFDQKLEGSASALVRMVRAFAAAGIANKVVAIFDNDAAAREELTTLKVAGLPDNIRVLRYPDIGLASDYPTLGPPTLESPDGSLARADVNGLAASIELYLGEDVLTLPDGSFRPVQWGALKKSGYQGVVSEKGEIQRLFREKLARAAADSSIVPSQDWTGMRMILDQILTAFRAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7U9</accession>
    <name>U5W7U9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34070"/>
    <dbReference type="EnsemblBacteria" id="AGZ45072">
        <property type="protein sequence ID" value="AGZ45072"/>
        <property type="gene ID" value="AFR_34070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6894"/>
    <dbReference type="eggNOG" id="ENOG5032QT3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139647_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="154" mass="17146" checksum="ACB16FFA2A8F7991" modified="2014-01-22" version="1">MTVHLAHYLGLLHRAQLTLADAFRQVAAAHGDEPDIDLLCRQQAERCDDHAARLEPFARRYAEDAPEEPERLHSELFRGTRTGGIGLLRDLQDLYLMAAECDVCWTVVGQAARGVRDRDLIEVVTSCDAETAIQLAWLRTRMKAAAPQALVVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7X3</accession>
    <name>U5W7X3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34220"/>
    <dbReference type="EnsemblBacteria" id="AGZ45102">
        <property type="protein sequence ID" value="AGZ45102"/>
        <property type="gene ID" value="AFR_34220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6924"/>
    <dbReference type="eggNOG" id="ENOG5031C56">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_775299_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="357" mass="38543" checksum="722A93D961FE0632" modified="2014-01-22" version="1">MPLAAAGLPAAPALADDVPMRGRVTGLLARDVTLVRPAGWDRLRGFVQPLTWAEAQPEPSGDVSEAALQKLRDAAGVARARGYTRLKLRLLAGYQSPAWALALGGGPMVGWEDPDPVTPLLYDVPVWWADDFLAAYTAFLPKLAAALEPEPLWGEVTVSGTCTVFAEPCIKQLGVAANRAKAIGREYTDTADRAALETVIAAHQDAFTPRGITSSVAYNPWQYIDPGTGRLKTDPDITIELMEFQKRTMGSFGVWENNSLIARRVDGRLEQTRADYQSMYDHMTAAGAAGHPIQFQTATLAKIEAEEDATVETTASWVAENHGISVELPRGWEQEATIDTGRATALNNRMVANAATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8G3</accession>
    <name>U5W8G3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560553.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29775"/>
    <dbReference type="EnsemblBacteria" id="AGZ44216">
        <property type="protein sequence ID" value="AGZ44216"/>
        <property type="gene ID" value="AFR_29775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6033"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164556_0_0_11"/>
    <dbReference type="OrthoDB" id="1864858at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="13030" checksum="0E24A6C4AD360E11" modified="2014-01-22" version="1">MTVTGPDFLALQVRDLERAAGFYEQHLGLRRIPQSPPGAIVFATEPIAFAVRNPLPGVDLDAGRPGLGVALWLHSPDAQALHDTLQSAGVPIVTPPFDSPFGRTFTFSDPDGYAVTIHDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8S0</accession>
    <name>U5W8S0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">AFR_35780</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561748.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35780"/>
    <dbReference type="EnsemblBacteria" id="AGZ45412">
        <property type="protein sequence ID" value="AGZ45412"/>
        <property type="gene ID" value="AFR_35780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7242"/>
    <dbReference type="eggNOG" id="COG0266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038423_2_0_11"/>
    <dbReference type="OrthoDB" id="1162346at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08971">
        <property type="entry name" value="AcNei2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="DNA_glyclase/AP_lyase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="MutM-like_N-ter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044090">
        <property type="entry name" value="Nei2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000214">
        <property type="entry name" value="Znf_DNA_glyclase/AP_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="SSF81624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51066">
        <property type="entry name" value="ZF_FPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FPG_CAT" evidence="16">
        <location>
            <begin position="2"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="FPG-type" evidence="15">
        <location>
            <begin position="226"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ45412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="29150" checksum="12F46DC38BF8CC12" modified="2014-01-22" version="1">MPEGDTVWNTARVLERAIQGEVLTGSDFRVPQLAAKDLTGWTVAESASRGKHLLLRLTRDGDQPLTLHSHLRMDGAWRAYAPGERWAGRPAHLIRVVLKTARSVAVGYHLHELALVPTADEDSLVGHLGPDLLGADWDPDEAVRRIAASPDVPIAEALLDQRNLAGVGNLYKAETLFLRGLWPWTPVSAVRNLEGAVKLAQKLVASNRGRWTQTTTGSLRRGQTSYVYGRRAQPCRRCGSAIQKEDQEERITYWCPRCQPKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8X9</accession>
    <name>U5W8X9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rpmB</name>
        <name evidence="6" type="ORF">AFR_36590</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561910.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36590"/>
    <dbReference type="EnsemblBacteria" id="AGZ45574">
        <property type="protein sequence ID" value="AGZ45574"/>
        <property type="gene ID" value="AFR_36590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7402"/>
    <dbReference type="eggNOG" id="COG0227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064548_7_0_11"/>
    <dbReference type="OrthoDB" id="2062238at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00373">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribo_L28/L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribo_L28/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001383">
        <property type="entry name" value="Ribosomal_L28"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00009">
        <property type="entry name" value="L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="63" mass="6732" checksum="BF5EF06B67A411CE" modified="2014-01-22" version="1">MASVCDVCGKGPGFGHNVSHSHRRTNRRWNPNIQSVRTPAGGGNTKKLKVCTSCIKAGKVVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W936</accession>
    <name>U5W936_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560838.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31225"/>
    <dbReference type="EnsemblBacteria" id="AGZ44505">
        <property type="protein sequence ID" value="AGZ44505"/>
        <property type="gene ID" value="AFR_31225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6320"/>
    <dbReference type="eggNOG" id="ENOG5033GD4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113744_2_0_11"/>
    <dbReference type="OrthoDB" id="2099859at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022062">
        <property type="entry name" value="DUF3618"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12277">
        <property type="entry name" value="DUF3618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="16341" checksum="C561B6899E7546F2" modified="2014-01-22" version="1">MSDNKNGSSGGKPDVAALREEIKQTRAELGETVQALAAKADVKARAKEQVEQTKQKARAQVHDATEKVRGAAAAAVGVASEKVRTATGQAGDKVAPALDKVRETAATAGDKARESGALDKVQEARVQVRRNPVPFAAVLAAGVALVGIILIVRGRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAF5</accession>
    <name>U5WAF5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39595"/>
    <dbReference type="EnsemblBacteria" id="AGZ46173">
        <property type="protein sequence ID" value="AGZ46173"/>
        <property type="gene ID" value="AFR_39595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8010"/>
    <dbReference type="eggNOG" id="ENOG5033055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168335_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="104" mass="11115" checksum="8896AF15DB99F581" modified="2014-01-22" version="1">MARVGAAPAARILHGVVLSRRWSAFLVVVGVWTWLIWPRFGLAIWKDDRSFADGSPTAFLWVHALLIGASLVIGTTVGVLGVRAWRGTRSAEEIGAPGGPAPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAG7</accession>
    <name>U5WAG7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561322.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33635"/>
    <dbReference type="EnsemblBacteria" id="AGZ44985">
        <property type="protein sequence ID" value="AGZ44985"/>
        <property type="gene ID" value="AFR_33635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6807"/>
    <dbReference type="eggNOG" id="COG1835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038663_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl_transf_3" evidence="2">
        <location>
            <begin position="11"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="366" mass="40455" checksum="9BA929FBFC79F3EB" modified="2014-01-22" version="1">MAEGTVVGRDRYFDTLRAVAIIRVVLFHAFPIAALELVFPSMGVMFALGGSLMVKSLDRSASRAVKNRVRRLLPALWVMGLIAVPLMLQQGWPDRPSWPHLLLWAFPIADPPSSEFGYPAAGVLWYLVTYLWLVLLSPLLLRLYRRWNLPTVLLPLFGLMLLTAYPNTFGETAGWVVQNVLAFGSCWVLGFAHREGDLAKLRPSTVLSLAAACVAGALGWAYLHPGENGIDLTGIPVAYTVFSIGFVLALLRWSPRMEWLARVRPIDGLVSMVNNRAVTIYLWHNVAITIAVMLFDPLELWRIPTQGLEDVVDFSIAITALAVAVLALGWVEDLAARRRPRLSPFVKRPELQQPAAPSHAPAPVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAM3</accession>
    <name>U5WAM3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="8">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpS</name>
        <name evidence="10" type="ORF">AFR_39625</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562512.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39625"/>
    <dbReference type="EnsemblBacteria" id="AGZ46179">
        <property type="protein sequence ID" value="AGZ46179"/>
        <property type="gene ID" value="AFR_39625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8016"/>
    <dbReference type="eggNOG" id="COG0180">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029244_1_1_11"/>
    <dbReference type="OrthoDB" id="951354at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_B">
        <property type="entry name" value="Trp_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024109">
        <property type="entry name" value="Trp-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="201"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="24"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="201"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ46179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="341" mass="37500" checksum="B617F7EB35314134" modified="2014-01-22" version="1">MSDADHRPRVLSGIQPTADSFHLGNYLGAVRNWVAMQDTADAFYCVVDLHAITMGHDPVALRNRTRLSVAQLLALGLDPERCTLFVQSHVPEHAQLAWVLSCITGFGEAGRMVQFKDKSSKQGSDTTSVGLFTYPILQAADILLYQADAVPVGEDQRQHLELTRDLAQRFNTRFGKTFTVPSAYIVRDTAKITDLQDPTAKMSKSASSPNGLIELLEEPARSAKKIKSAVTDTGREILYDEENKPGISNLLTIYAALTSRTIDELLEQYDGRGYGDLKKDLAEIVVEFVKPIQERTRTFLEDPGYLDKILAVGADKARAVSAVTLADAYQHLGFLSPSRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAP6</accession>
    <name>U5WAP6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Twin-arginine translocation pathway signal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561368.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33865"/>
    <dbReference type="EnsemblBacteria" id="AGZ45031">
        <property type="protein sequence ID" value="AGZ45031"/>
        <property type="gene ID" value="AFR_33865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6855"/>
    <dbReference type="eggNOG" id="COG1879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_3_3_11"/>
    <dbReference type="OrthoDB" id="1378040at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666244" evidence="1">
        <location>
            <begin position="23"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_4" evidence="2">
        <location>
            <begin position="41"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="33541" checksum="00C6C74D45EC7494" modified="2014-01-22" version="1">MRLNRRSTAVGAAALLLTSSIAACGGGDDAGSGDKTSGFKIALIPGVANDAYYGSVACGVQAAAKASSSTVDVQAPKSFSPTDQIPILQAVIAKKPDVIIIAPTDSKALYAPLKQATAQGIKVVLVDTTLEDPSIAEAQVSSDNIEAGKTAAAKLIELLGSKAGSVLTVNLSAGVTTTDDRFKGFAEGLKAAPQLKDLGQQFSNNSVQTASSIVSATVASNPDLIGIFSTAAFNTEGAVAALRAAKDDRVTIVGFDANPPGVKQIQDGEVAAQVVLKPYDEGVAAGEQAINALTGKPVTKVISTGAVVATKDNLTSPEVTKYLYSFDCPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAU8</accession>
    <name>U5WAU8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative flagellar hook capping protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">flgD</name>
        <name evidence="4" type="ORF">AFR_40470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FlgD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562680.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40470"/>
    <dbReference type="EnsemblBacteria" id="AGZ46348">
        <property type="protein sequence ID" value="AGZ46348"/>
        <property type="gene ID" value="AFR_40470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8191"/>
    <dbReference type="eggNOG" id="COG1843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047535_1_1_11"/>
    <dbReference type="OrthoDB" id="709873at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005648">
        <property type="entry name" value="FlgD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03963">
        <property type="entry name" value="FlgD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="145"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="20396" checksum="EBE002EC0E994B83" modified="2014-01-22" version="1">MTSPIGGNVSKEFDAKNVSATSMYTNRTTTDSGASKSVADQDTFLKLLVAQLKYQDPSNPADSTQFLAQTAQFTQVEKLGQIAEMMQAQQLIGASALVGRTVTFQDANGTTQTGVVTKTKLNGDSEPTLVVGNTDVQLSKVTEVQQATTGPATTGQTATTPATPAAPPATTPAPAATKPAEPAEPATTSDDSATTKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAW7</accession>
    <name>U5WAW7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_34285</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34285"/>
    <dbReference type="EnsemblBacteria" id="AGZ45115">
        <property type="protein sequence ID" value="AGZ45115"/>
        <property type="gene ID" value="AFR_34285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6937"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="19"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="110"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="177" mass="20106" checksum="78B702E074780AC4" modified="2014-01-22" version="1">MLVTSERDQQFHDFVRARRAGLVRTATLLTAGDAHLAEDLVQSTLTKLYVAWPAFQRAHSPEGYVRRVLVNALTDERRRLWWRRERPTAELPDRPHTAAEPARGELDDGLRTALRDLPPKMRAAVVFRYFYDLDVAETADALGCSHGTVKSQTARALDRLREALQSNALFLRQGVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB27</accession>
    <name>U5WB27_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase class C-related penicillin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561473.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34390"/>
    <dbReference type="MEROPS" id="S12.950"/>
    <dbReference type="EnsemblBacteria" id="AGZ45136">
        <property type="protein sequence ID" value="AGZ45136"/>
        <property type="gene ID" value="AFR_34390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6958"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_11_2_11"/>
    <dbReference type="OrthoDB" id="1439085at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="378" mass="40268" checksum="E430A830CA695623" modified="2014-01-22" version="1">MSSERIEAALRPYVENGHLPGAVALRARGAEVDVACVGVRDLGTGVPMTRDTLFRIASLTKPLVAAVALMLVEDGTLALDKPVDEWLPELAGRPVLRELGAELDDVVPLARPITVQHVLTYTHGYGAVMAPPGTYPVQERIEALQLGPGADPEPFSRDEWMARLGTLPLLHQPGEGWSYHGGGEILGALLQRATGLSLETLLHERLFEPLGMRDTSYACIDPERLATAYRPGMSGELEVCAEPEGRWSPPLRVSGATGLISTVDDCLAFGRMLLDGGGGLLSDGSVAQMTTDRLTAQQKAAAMWLPGFWDGWGWGFGGAVATGREVPGPAAGSYGWTGGTGTACYTDLRGGRVGVLLTQREMTSPMPDAYANAFWEAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB47</accession>
    <name>U5WB47_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-glutamate O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_40575</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562701.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40575"/>
    <dbReference type="EnsemblBacteria" id="AGZ46369">
        <property type="protein sequence ID" value="AGZ46369"/>
        <property type="gene ID" value="AFR_40575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8212"/>
    <dbReference type="eggNOG" id="COG1352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025854_0_2_11"/>
    <dbReference type="OrthoDB" id="1378151at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="31178" checksum="7F512F718FD0ADAF" modified="2014-01-22" version="1">MTLSAADFTFVSTLVRREAAIVLAPGKEYLVEARLIPVARQVGAASVAEFLANLQRRPNPADQRRIIDALTTNETSWFRDREPFSALTDVVLPELVRRPGANRKVRIWSAASSSGQEAYSLAITLQENLPAGWSYEIMGSDISTEMIKRAEAAEYSQVEVNRGLPAAQLVQYFERAGAHWRITQSLRRNVSFKLMNLTAPLPPMPPFDVIFLRNVLIYFDVATKKSVLRNVARLLRPDGWLFLGAAETTIGIDDNYERVAAGRTSAYRVRSAVPAGAARKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBI5</accession>
    <name>U5WBI5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trk system potassium uptake protein TrkA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_35275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35275"/>
    <dbReference type="EnsemblBacteria" id="AGZ45311">
        <property type="protein sequence ID" value="AGZ45311"/>
        <property type="gene ID" value="AFR_35275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7137"/>
    <dbReference type="eggNOG" id="COG0569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="6">
        <location>
            <begin position="137"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="24044" checksum="2D819595A84563FF" modified="2014-01-22" version="1">MRIAIAGAGNVGRSIAQELIGNGHQVMLIERQPRQLRPERVPEAEWVLADACEVSSLEEADVAACDVVVAATGDDKANLVCSLLAKTEFAVGRVVARVNRAENEWLFTEQWGVDVAVSKPRLMAALVEEAVTVGDLVRIMTFRQGEANLVEITLPTTAPYVGQPVRAVPMPRDSALVAILRGKRVLVPTPDDPLEAGDELIFVCTAEVEDAVRAVVLGSARELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBM0</accession>
    <name>U5WBM0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative Fur-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562724.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40690"/>
    <dbReference type="EnsemblBacteria" id="AGZ46392">
        <property type="protein sequence ID" value="AGZ46392"/>
        <property type="gene ID" value="AFR_40690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8235"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_4_3_11"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="141" mass="15588" checksum="728E06485F051328" modified="2014-01-22" version="1">MSATSLAEILRERGLRLTPQRQLILEAVHELGHATPEQIHHTVRERAAGVNITTVYRTLELLEDLTLVSHTHLSHGSPTYHAAGEDQHVHLVCRNCGSIDEVDPSVMQPVTVQLQQDRGFRVDVGHVSLFGECGDCKETPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC18</accession>
    <name>U5WC18_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561837.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36225"/>
    <dbReference type="EnsemblBacteria" id="AGZ45501">
        <property type="protein sequence ID" value="AGZ45501"/>
        <property type="gene ID" value="AFR_36225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7330"/>
    <dbReference type="eggNOG" id="ENOG502ZNCM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363792_0_0_11"/>
    <dbReference type="OrthoDB" id="2071842at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="24303" checksum="B5316339E7AB0025" modified="2014-01-22" version="1">MEGHRRYAEEPEPSWYTGQRPSDPAAANAYDSGVYERPSGAFRLPEQRPADPYATPAGYSPPDPVTTSGSHALSSSDSGSIRMPVRGPEYPTVRPTSAAAADPPAVSTVTTTTTYGSGVTPESPPPAYNQPTSMVPMASAAPKRPVSALVFAVLTVVLLIPAVLLLVQATFVDDPASRGIVPAVLLTLGLPMTGLGLYTLAGIKTSGRDAWLRPPLVYLPIGLILLLAAGLGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCA5</accession>
    <name>U5WCA5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative diguanylate cyclase/phosphodiesterase with GAF sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562928.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41715"/>
    <dbReference type="EnsemblBacteria" id="AGZ46597">
        <property type="protein sequence ID" value="AGZ46597"/>
        <property type="gene ID" value="AFR_41715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8442"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_34_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="345"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="1">
        <location>
            <begin position="486"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="744" mass="79169" checksum="EAC84CD72E80825C" modified="2014-01-22" version="1">MFTWSTHQLTEFFTAVTASADENAATAVAVERAAEMVEAEAGAVVLDEEIHSAWGFGPEVPVKGLLATVQTPGTIPVPGVGDLYAVPSALGSHLRGALIVARLDDPFTPEERQMLQGMAQVLGLALRSIRVLRAERTLRAEREREAAERLVLLEQAQTRRLLVETLLTIQREISNRKPLPEILDAVTSGTAALLGGAPVALILAEAGRARRLSIASTSGAGGYDFADPEGSHTEAREAMATGAVIMQPADPEDPEWGTVIAAPVRVTGEMAGSLVAQLPGAADRYAEQRDQLAAFAQQVSLALTDARTVEAVREAHHDAVTGLPNRGLFLKIFNRVLASRAAATEPTSVLFIDLDRFKAVNDSLGHEAGDQLLAAVAGRIRGCVRASDTTARLGGDEFAVLLHNSPVEAATAVGERVISAIKEAFRISGRDIFIGASVGVAISREPTADSNGLLNKADVAMYRAKKDGPGKVVVYEPHMHTEALNYLSLRGDLQRALAENEFRLQYQPLVRLDSGDIAGVEALIRWHSPTRGLVSPADFIPIAEESGLIVDIGQWVLESSAAQVAAWRRNSPDLTLNVNVSGHQLVHPRFAANVTRALAVAGLPSSAVTLELTESVLMSDPDAAVASLASLRELGVQLSIDDFGTGYSSLAYLRELPVDELKIDRAFIARAELTSEDLALVRTIVELGHILGLRVVAEGIENAAQLEALRRLGCSYGQGYHLCRPSDPADLPKILAGGPVAPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCW6</accession>
    <name>U5WCW6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562111.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37610"/>
    <dbReference type="EnsemblBacteria" id="AGZ45776">
        <property type="protein sequence ID" value="AGZ45776"/>
        <property type="gene ID" value="AFR_37610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7607"/>
    <dbReference type="eggNOG" id="COG4319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142180_0_0_11"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="121" mass="14262" checksum="79D9D3952437A9A8" modified="2014-01-22" version="1">MTSLDAVTAWVHRYRVAWESNRPDHIGGLFAEDAAYFPEPYAKPWLGRDEIIAKWLEAKDEPGETTFEWHPLIVTDDLAIIEATTVYREPPRTYSNMWVLRLDISGQARQFTEWWMQHPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCX6</accession>
    <name>U5WCX6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_42890</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563162.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42890"/>
    <dbReference type="EnsemblBacteria" id="AGZ46832">
        <property type="protein sequence ID" value="AGZ46832"/>
        <property type="gene ID" value="AFR_42890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8677"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_4_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014325">
        <property type="entry name" value="RNA_pol_sigma-E_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02983">
        <property type="entry name" value="SigE-fam_strep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="18"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="103"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="19396" checksum="A56914FD753D67D0" modified="2014-01-22" version="1">MEQTVEAAFTTFVAERSHALIRVAYTLTGDQRAAEDLVQGALAKAYSHWSRIHGDAEPYVRRIIYNDKVSGWRRQARQRELPMADVPEVAESRDHDHDTALRLVLRQALLSLPPRQRAVLVLRYLEDRSVEETAEVLGCRPGTVASQASRALGKLRELVPELGSADEAAEVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDU4</accession>
    <name>U5WDU4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-acetyltransferase GCN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39135"/>
    <dbReference type="EnsemblBacteria" id="AGZ46081">
        <property type="protein sequence ID" value="AGZ46081"/>
        <property type="gene ID" value="AFR_39135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7920"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104207_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="39"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="197" mass="21934" checksum="688E60CB979DFE45" modified="2014-01-22" version="1">MAGLSDSSSAFRPGGERRDGGDGRAIDRHDDPVGGLGTLRIAPRAPGDIDRLGRTFGPQAQSYYLERSRRQGVLLVARVGERAIGAVFVSTEPAPESAIIRRLGKVPMLHKLMVDEQLRRRGIGTRLIRSAEAELRRLRQHRVAVGVDIDNPSAARLYQRLAYREWAYGLLETVREDVKDGKVILLPDECRVFLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEX8</accession>
    <name>U5WEX8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_41505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41505"/>
    <dbReference type="EnsemblBacteria" id="AGZ46555">
        <property type="protein sequence ID" value="AGZ46555"/>
        <property type="gene ID" value="AFR_41505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8398"/>
    <dbReference type="eggNOG" id="ENOG5034315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117051_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="158" mass="17731" checksum="83B20F9B6310C202" modified="2014-01-22" version="1">MPGHGWRADLRADEKVVQGSRTYVPVMPEAEWYRAEAEQTEVFAPLVPVERVWVEELGMAGTPAKPGDVMSRLVSLDEPPRRNPVAALDADALTGHRVVQLLEDGGERRELRAVTELHTSAEGDICARVATELDWYRWGWSGQAPRTLEVPVHLLWIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF25</accession>
    <name>U5WF25_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562956.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41855"/>
    <dbReference type="EnsemblBacteria" id="AGZ46625">
        <property type="protein sequence ID" value="AGZ46625"/>
        <property type="gene ID" value="AFR_41855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8470"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1703201_0_0_11"/>
    <dbReference type="OrthoDB" id="1735450at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="16"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="16995" checksum="E6858C1A611A6DB6" modified="2014-01-22" version="1">MKVLVWVEEGTWPACVDAARDLSDDITLLHVVDEATVAAMSGPVGLLGRSGGGGDPEFLLAEAERTLIDEAVARLGREVPREVRRGRPEREVVAACADVDLLVLARNGDRSRLGPRSLAHHTRFVVDHAPCQVLLVWPEATPGLGTIPPPPHHDRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF88</accession>
    <name>U5WF88_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_41585</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41585"/>
    <dbReference type="MEROPS" id="S01.513"/>
    <dbReference type="EnsemblBacteria" id="AGZ46571">
        <property type="protein sequence ID" value="AGZ46571"/>
        <property type="gene ID" value="AFR_41585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8415"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1286">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043139_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009403">
        <property type="term" value="P:toxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003825">
        <property type="entry name" value="Colicin-V_CvpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02674">
        <property type="entry name" value="Colicin_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="390" mass="40688" checksum="F153A10A4B68F749" modified="2014-01-22" version="1">MVDGILILLMLVFAISGYRQGFVIGALSFGGFFSGVLIGLQVGPLIANQFADGTIRLVVSLVAIFALAVLGQTLAGWLGTKLRRSISSQPLQRLDDAGGAVVSLVAVLLVAWLIAVPLGSTPFPGINKQVRSSAVLNGINSLMPEQAQALSAGLRESLNTNGFPDVFGGLARTNAREVAAPDPKLAKSPVVVNSRRSVIKVLGTAPSCSRRIEGSGFVYSDERIMTNAHVVAGTRNVQVETNDGRLDGKVVVYDPDRDLAVIYVPGLRAPVMPFVERPAATGANAIVLGYPLDGPYDAQSARVREVSKITGPDIYDSGDVTREIYTIFAKVRSGNSGGPLVAPNGDVLGVIFAAAADDQTTGFALTAAEASGTARLGIERTRGVKTGDCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFF6</accession>
    <name>U5WFF6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Osmotically inducible protein OsmC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563101.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42580"/>
    <dbReference type="EnsemblBacteria" id="AGZ46770">
        <property type="protein sequence ID" value="AGZ46770"/>
        <property type="gene ID" value="AFR_42580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8617"/>
    <dbReference type="eggNOG" id="COG1764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106355_1_0_11"/>
    <dbReference type="OrthoDB" id="1655733at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019904">
        <property type="entry name" value="Peroxiredoxin_OsmC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03562">
        <property type="entry name" value="osmo_induc_OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="142" mass="14730" checksum="FCB8B48445404523" modified="2014-01-22" version="1">MPIRTASARWSGNLTEGSGTIKTGKGGYEGNYSFKSRFEEGEGTNPEELIGAAHAGCFSMAFSKGLADAGFTPTSVETTAKVHLDKGDAGFGVTRIDLETVGDVPGIDEGTFQKIAEGAKENCPISRLLSPGAEITLSAKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNH3</accession>
    <name>U5VNH3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_00290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357291.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00290"/>
    <dbReference type="EnsemblBacteria" id="AGZ38347">
        <property type="protein sequence ID" value="AGZ38347"/>
        <property type="gene ID" value="AFR_00290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.59"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_44_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="12"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="273"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="273"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="360"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="462" mass="47883" checksum="553D079F8CCBE3C0" modified="2014-01-22" version="1">MISPGVTLGGRYRLDERIAGGGMGDVWRGTDEVLGRTVAVKILLPALLDEPGFAERFRGEARTMATINHPGVVDVYDYGSDQQLAFLVMEYVEGDALSRTLSRVGRLTPARTMALVAQAADALQAAHANGIVHRDVKPGNLLVRPNGTLVLTDFGIARSAMVGQLTVAGSVLGTASYISPEQASGAVATAASDVYALGVVAYQCLSGHRPFDGSTPIEIAMKHVRDTPRPLPADIPQAVRAIVDRALAKDPGARWPTASAMAAVARQAASSLTTQVQQPAVNGSSAGRPQSGGPTMTRPQSSGPSMANRPHSGAPTSGPGGHPMSGQGRPPYPPIPRPVSGAQARGAASVPPAQQPYRHPSAPPMQQHQPMQGGKQADGSGGRQVLIVLAVVLALLVLLCAGVISFLLNQGNTNAMGDHGNRSTVVVAGSADQRTSATSYRLTKQAAAPHSLNHATEGRQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPB6</accession>
    <name>U5VPB6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01790"/>
    <dbReference type="EnsemblBacteria" id="AGZ38647">
        <property type="protein sequence ID" value="AGZ38647"/>
        <property type="gene ID" value="AFR_01790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.362"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="33"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="33515" checksum="59DFDB4266756736" modified="2014-01-22" version="1">MDETCVLTEGPWTHRFVGANGSRFHVVEAGTGPLVLLLHGFPEFWWTWHDMLTRIADAGFRAVAIDLRGYGASDKPPRGYDGYTMAADVTGLIRALGERSAMIVGAGAGGMIGWAAAAFHPKMVSRLVVLGAAHPLRLRAALFADPRGQFSASAGLLRFQVPRYEHVLTKDDAALVGELIERWSSPRWTETPEYAEYVARCREAIQIPQAAFCALEAYRWVFRSALRLQGYRFVKLMQQPLVTPTLQMHGALDTAVLPRTAQGSGRYVIAPYEWRLIEDAGHFIHQEAPDVVTGEVLRWLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPI3</accession>
    <name>U5VPI3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02945</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357821.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02945"/>
    <dbReference type="EnsemblBacteria" id="AGZ38878">
        <property type="protein sequence ID" value="AGZ38878"/>
        <property type="gene ID" value="AFR_02945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.592"/>
    <dbReference type="eggNOG" id="COG0392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048247_0_0_11"/>
    <dbReference type="OrthoDB" id="1831905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="342" mass="35748" checksum="D18B1D5017527227" modified="2014-01-22" version="1">MVVSDAGAAPAWWRRGPAVAAVIVLILGAELVLGWSALVQAVHQLRTPAWNWVAGALIAEIASMGTYARMQRALLRGAGTKVPVRRHVALAYAAHSLSATLPGGPVFSTAFNFRQMRRYGASSAVASWCIALSAVLSTGALVVIGAVFGLLTRSTNSWHTLLLYAGGALVVAFGVRFLAEHPEWLIGPARKLLSGLNRLRRQPADTGHARLVGFVDQLRSVRVRPADLTLAIVLAVANWLLDALCLWMCCIAVGAEGINPVQLVIAYCAGMAAASVPIVPGGLGVVDGALVLGLVAGGLTSSSAVAAVVLYRVISFGFIIGAGWLVWLIMRTRAGTPRGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPJ7</accession>
    <name>U5VPJ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phage shock protein C, PspC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_02240</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02240"/>
    <dbReference type="EnsemblBacteria" id="AGZ38737">
        <property type="protein sequence ID" value="AGZ38737"/>
        <property type="gene ID" value="AFR_02240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.453"/>
    <dbReference type="eggNOG" id="COG1983">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143433_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007168">
        <property type="entry name" value="Phageshock_PspC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04024">
        <property type="entry name" value="PspC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="PspC" evidence="7">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="63" mass="7104" checksum="6918ED5F3B30FD5C" modified="2014-01-22" version="1">MSDLARRLARPRDDRRIAGVCSGLARRFGMSSGMVRLIFVLSCLLPGPQFLIYIILWALLPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPT4</accession>
    <name>U5VPT4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Drug resistance efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_02625</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02625"/>
    <dbReference type="EnsemblBacteria" id="AGZ38814">
        <property type="protein sequence ID" value="AGZ38814"/>
        <property type="gene ID" value="AFR_02625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.528"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="495" mass="50562" checksum="5F88F9A4790E1815" modified="2014-01-22" version="1">MLLATVLGSSLAFIDATVVNIALPAIGRDLDSSAAGLQWTVNGYALSLASLILLGGSLGDRFGRKRVFMIGIGWFALASLLCGIAPNIETLIAARTLQGIGGALLTPGALAILEASFVREDRAKAIGAWSGLGGIGGALGPFLGGWLVEVANWRFIFLINVPLALLVMVVAARHVPESRNPNAARRLDVTGVLTGAAGLGGLTYGFTAWPANGPGSPQVLVSLLIGVAGLAAFVLTERRSAHPMLPLGVFSSKAFSGANLVTFLVYAANGGVFFLVVLNLQVVSGFSPLTGGLALLPVTALMLVLSARAGALGQRIGPRLPMTVGPLICAVALAWMSRIGVGASYWTDVLPPVIIFGLGLSLLVAPLTATALGALDDTFAGIASGVNNAVARAAGLLSVAVLPLAAGIGTGNLTDAQDLHPTYRNSMLICAALMVIGGLLAAVLIPRRLPAHTPAKRSPVDGRDVVTIEPVGGDQSPVQTYCDMAGPPVHPRSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQD2</accession>
    <name>U5VQD2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_03665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357967.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03665"/>
    <dbReference type="EnsemblBacteria" id="AGZ39022">
        <property type="protein sequence ID" value="AGZ39022"/>
        <property type="gene ID" value="AFR_03665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.738"/>
    <dbReference type="eggNOG" id="ENOG503260V">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2091551_0_0_11"/>
    <dbReference type="OrthoDB" id="2176976at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665141" evidence="2">
        <location>
            <begin position="32"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="116" mass="12122" checksum="6A59D006FB1C08F8" modified="2014-01-22" version="1">MKVKLNKRQLLLGAGVVVLVLGGLAVGRASQDEGPGTLDDAAVQACNDFAAGEDDARSKTQRLALADRVNKSSAESDNKTIAERGAAMGRSANDSTKVWRADADAFTKACRDAGWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQG5</accession>
    <name>U5VQG5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative ABC transporter ATPase and permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_03750</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03750"/>
    <dbReference type="EnsemblBacteria" id="AGZ39039">
        <property type="protein sequence ID" value="AGZ39039"/>
        <property type="gene ID" value="AFR_03750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.756"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_9_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="29"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="345"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="378"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="573" mass="61057" checksum="FFB57699AC83FC83" modified="2014-01-22" version="1">MSAALPIADQRLVSRAALRLIAADRRTVVVMMILNVLAAIAGLGGPWLLGRIIDTVTGGQGGGQGAGQVDRLAIAVLGCAVAQALLSRYALSVGYRFGERTAARIRDDFLRRSLELPAAVVERVPAGDLTARGTTDVDAVATTLRDVVPGLFISAVQLVLIVTAVVVLDPALGAVGVLGFTGIWFVTRWYLSRARDAYLLEGAANSVMADELAATTTGARTIEAFDLRERRLGRGREAIAETRRTRLATLSLRSVFFPLVELSYVVPIVLVLLAGGWLYFRGSVTLGTVAAAVLYLRQLVGPVDNIMLWIEQLQSSGASFARIEGLAAVPTDQTTSSARPDGDKIEVRGVRYAYDSGQDVLHDVDLTIRPGERLAIVGLSGAGKSTLARLIAGVDRPDHGSVTVGGVPIADLPPDELRRQVVLVTQEHHVFRESLRENLMVVAPDDVLKRALKTVGADWADDLDADLGENPPDGGQAQQLALARVVLADPHTVILDEATALLDPTAARSAERALAAVLHDRTVIAIAHRLQTSHDADRVAVMDGGRIIELGSHDALIRADGPYAALWRSWHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQS0</accession>
    <name>U5VQS0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">BTB/POZ domain-containing protein KCTD9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358829.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05120"/>
    <dbReference type="EnsemblBacteria" id="AGZ39313">
        <property type="protein sequence ID" value="AGZ39313"/>
        <property type="gene ID" value="AFR_05120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1036"/>
    <dbReference type="eggNOG" id="COG1357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033401_5_5_11"/>
    <dbReference type="OrthoDB" id="1677662at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13599">
        <property type="entry name" value="Pentapeptide_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="197" mass="21008" checksum="72A5C0A1FDB064ED" modified="2014-01-22" version="1">MDVVRNETFSGQDWALAEVERAHYEDCAFHDVDWSEAKLSGCTFTHCEFGNVRFNAAELTTCAITQSVVRRSSFFDATLTGCKLTGTQFLDCGLRPLTIDGGDWGFVSLRGANLTAAKLGGLKLREADLTNADLTRADLRGADLSHARLKDAVLKGADLRGADLDGVALRGVALDGAKIDLAQAALFATAHGAQVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQY9</accession>
    <name>U5VQY9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative ABC transporter ATPase and permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_04875</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358308.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04875"/>
    <dbReference type="EnsemblBacteria" id="AGZ39264">
        <property type="protein sequence ID" value="AGZ39264"/>
        <property type="gene ID" value="AFR_04875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.984"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="33"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="260"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="561" mass="59493" checksum="3B5F138D048A1896" modified="2014-01-22" version="1">MRELPPADPGIPDDRSATRYLGWLARRMWVSVVGGIVFAIIWMTCQALVPAIVGRAIDAGLTGRNRHDLLVWSLVLLGVGLVQAGAGVIRHRLTVFNWLSAAYRTVQVVVRQTNRLGSALPRRLDAGEVVAIGTADISHIGGALDITARGAGSLVAIIIVTVILLVTSVPLGLIVVIGVPVLMAVVGLLIRPLHHRQQAYREQQGRLTGRAADLVGGLRVLRGVGGEPMMSGRYRRDSQKLRAAGVREAQVESLLEAAQVLLPGIFLVLMTWLGARFALSGRITIGQLVSFYGYAAFLVAPLRQLTETLDKMTRGHVSGRRVVAMLRARTDLPDPERPAMSPGPGKLVDPESAAVVRPGLVTAIAAAAPADAVAIADRLGRYVDTTATLSGVPLDDLDLATVRDRILVADNDARLFSGPLREDLDPSGSAPVEAALAAAGADEIVAALPDGLDSEVEERGRTFSGGQQQRLRLARALVADAEILVLVEPTSAVDAHTEARIADRLGAARRGRTTVVCSTSPLVLDRADVVLYVEAGAVVAEGTHRELLGSCAGYARTVLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRU9</accession>
    <name>U5VRU9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11 12">Xylose isomerase</fullName>
            <ecNumber evidence="4 11 12">5.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">xylA</name>
        <name evidence="15" type="ORF">AFR_07020</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11 12">
            <text>alpha-D-xylose = alpha-D-xylulofuranose</text>
            <dbReference type="Rhea" id="RHEA:22816"/>
            <dbReference type="ChEBI" id="CHEBI:28518"/>
            <dbReference type="ChEBI" id="CHEBI:188998"/>
            <dbReference type="EC" id="5.3.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11 13">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the xylose isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.5" evidence="4 11 12"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359224.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07020"/>
    <dbReference type="EnsemblBacteria" id="AGZ39693">
        <property type="protein sequence ID" value="AGZ39693"/>
        <property type="gene ID" value="AFR_07020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1432"/>
    <dbReference type="eggNOG" id="COG2115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060750_0_0_11"/>
    <dbReference type="OrthoDB" id="481478at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009045">
        <property type="term" value="F:xylose isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00455">
        <property type="entry name" value="Xylose_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013453">
        <property type="entry name" value="XylA_actinobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001998">
        <property type="entry name" value="Xylose_isomerase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00688">
        <property type="entry name" value="XYLOSISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02631">
        <property type="entry name" value="xylA_Arthro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51415">
        <property type="entry name" value="XYLOSE_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0859">Xylose metabolism</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="14">
        <location>
            <begin position="41"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ39693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="395" mass="43704" checksum="7E6BAE9802B55BF4" modified="2014-01-22" version="1">MSVQPTREDKFSFGLWTVGWTARDPFGDATRAPLDPIEAVHKLSELGAYGITFHDDDLVPFGSDAPARDGVIANFKKALDETGMVVPMVTTNLFTHPVFKDGGFTSNDRSVRRYALRKVLRNMDLAADLGAKTFVLWGGREGSEYDVAKDVQAALDRYAEGLNLLAQYSEDNGYGLRFAIEPKPNEPRGDILLPTAGHAIAFVNDLERPELFGINPEVGHEQMSNLNFAQGIAQALWHGKLFHIDLNGQHGPKFDQDLVFGHGDLLNAFALVDLLEHGGPGGGPGYDGPRHFDYKPSRTEDYTGVWESAKANMRMYLLLKERAQAFRADPAVQEALAESKVLELSKPTLNEGETYTDLLNDRSAFEDYDVDAAGEQGYHFVKLHQLAIDHVLRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRV7</accession>
    <name>U5VRV7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_00930</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00930"/>
    <dbReference type="EnsemblBacteria" id="AGZ38475">
        <property type="protein sequence ID" value="AGZ38475"/>
        <property type="gene ID" value="AFR_00930"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00930"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.185"/>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065805_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="182"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="29610" checksum="B304B8D43312B4E9" modified="2014-01-22" version="1">MVRKAIFQLEQADQLDAVSDRLQGIVQRVAKPKKLRDLLHGTWLGHPLHPVLVQAPVGAFMSAAVLDALPGQRRAATTLIAFGTAAAAPAIAAGWVDWSSLSRDQRRVGLVHASANAVALLLYGGSLAARFSGRHGLGKVLAYAGLSLAGGGAYLGGHLSYEQGAGMNQAAVELMQLPEDWAEVGEVSSLPEGKPTVRTIGDVRVLLYRLGDEVSAMIEHCGHQGGPLGEGDVEGSGADACVVCPWHGSTFRLVDGAVMHGPAAGDQPTLRTRVRDGVLSVATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS32</accession>
    <name>U5VS32_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LapA_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_07520</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07520"/>
    <dbReference type="EnsemblBacteria" id="AGZ39793">
        <property type="protein sequence ID" value="AGZ39793"/>
        <property type="gene ID" value="AFR_07520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1535"/>
    <dbReference type="eggNOG" id="COG5416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134414_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010445">
        <property type="entry name" value="LapA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06305">
        <property type="entry name" value="LapA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="LapA_dom" evidence="7">
        <location>
            <begin position="75"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="13557" checksum="09E41F5F8B520D1D" modified="2014-01-22" version="1">MAYPSENRPFTAPQDDAPPPLPPELAGTLAGLEGAGEAPPRRPDSRVPRTRTSAVWFGVWAGAVALILLIIFVAQNTGNVRLSFLWMDGQIPLALAMLIAGVCGAFVAMAVASVRIIQLRRLVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS83</accession>
    <name>U5VS83_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">zf-CGNR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357469.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01185"/>
    <dbReference type="EnsemblBacteria" id="AGZ38526">
        <property type="protein sequence ID" value="AGZ38526"/>
        <property type="gene ID" value="AFR_01185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.236"/>
    <dbReference type="eggNOG" id="COG5516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087298_0_0_11"/>
    <dbReference type="OrthoDB" id="1478254at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010852">
        <property type="entry name" value="ABATE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023286">
        <property type="entry name" value="ABATE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021005">
        <property type="entry name" value="Znf_CGNR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35525">
        <property type="entry name" value="PTHR35525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07336">
        <property type="entry name" value="ABATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11706">
        <property type="entry name" value="zf-CGNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160904">
        <property type="entry name" value="SSF160904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-CGNR" evidence="1">
        <location>
            <begin position="148"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="22980" checksum="A09FB5D73231D118" modified="2014-01-22" version="1">MSWKASARHGVREAPAGLALLQDLLNTRASMSYAPDLLGTPDDAQRWLTDALGTWARVAGLPAPVILLSSTDMRSLRRLRTTFEGVILAGGRSPEPDGALPPADVPVSLVPDADGWVRMVPSGRGIRWLASALWAEALLAQQAGLWPRLKLCHNIDCRAAFFDTSRNNSGVWHDVSTCGNTANLRAFRERKRLMGNSGNQLGGPSVQRPDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS91</accession>
    <name>U5VS91_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_07175</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359255.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07175"/>
    <dbReference type="EnsemblBacteria" id="AGZ39724">
        <property type="protein sequence ID" value="AGZ39724"/>
        <property type="gene ID" value="AFR_07175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1464"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="322" mass="33599" checksum="2EE36F11ECF9CBBF" modified="2014-01-22" version="1">MSAPAIETTGLIKTFGDNRAVDGVDLTVPPGTVYGLLGPNGAGKTTVVRMLATLLRPDGGQARVFGHDVVREADAVRSRVSLTGQYASVDEDLTGTENLILLARLLGYAKPAARERAAQLLEAFGLTDAAARQVKKYSGGMRRRIDVAASILNTPDLLFLDEPTTGLDPRSRNQVWDIIRVVVANGTTVLLTTQYLDEADRLAGRIAVIDHGKVIAEGTPGSLKASIGAGTVHVRLRDAAQRSQAAGLLADALGTTVQEEADPVALTARASGSGSERGAAEQASRALGALADAGIIVDDFSLGQPSLDEVFLALTDRPEVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSK8</accession>
    <name>U5VSK8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357554.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01610"/>
    <dbReference type="EnsemblBacteria" id="AGZ38611">
        <property type="protein sequence ID" value="AGZ38611"/>
        <property type="gene ID" value="AFR_01610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.324"/>
    <dbReference type="eggNOG" id="COG4279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053146_2_2_11"/>
    <dbReference type="OrthoDB" id="1906220at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="31079" checksum="953BBE29BDF1101D" modified="2014-01-22" version="1">MPVDPEGRWFDSTGPIHVDGGLAVRAKRGKIGEQWWSRRFVDILEQTCDAGRLARGRSYARKGQVMDFELTPGQVTGRVQGSRPEPYQVTISIPAFDKPQWTRILDALGSQALYRAALLAGDMPHELVDVFDELELPLFPSRLDMDCSCPDWGKPCKHVSAVLYVLAEAFDDDPFLVLAWRGRGREELLAALRGLPEPVETIDPLAVDEVALETRLHDFYTPGISLSRLRERPGRVTVPPELLLRVLDSPQVRVRHIPIVDILRPAYRDLAEPQGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSL5</accession>
    <name>U5VSL5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359338.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07590"/>
    <dbReference type="EnsemblBacteria" id="AGZ39807">
        <property type="protein sequence ID" value="AGZ39807"/>
        <property type="gene ID" value="AFR_07590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1549"/>
    <dbReference type="eggNOG" id="ENOG5033P21">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125367_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025327">
        <property type="entry name" value="DUF4233"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14017">
        <property type="entry name" value="DUF4233"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="12351" checksum="CA54487C9DE2CC2E" modified="2014-01-22" version="1">MTTPPPEPKPSGLRNPAAAVRGLGAGTLALEAIVLLLAIQPIRIFAGNLSGWGVGAVVALAVLAAALAGSMRRPWAWHAGSVLQVLLLASGLLHWSLAALGLIFGTAWLYATSVRRKILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTE0</accession>
    <name>U5VTE0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Spheroidene monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359960.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09845"/>
    <dbReference type="EnsemblBacteria" id="AGZ40258">
        <property type="protein sequence ID" value="AGZ40258"/>
        <property type="gene ID" value="AFR_09845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2007"/>
    <dbReference type="eggNOG" id="ENOG5030G1W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064051_0_0_11"/>
    <dbReference type="OrthoDB" id="1663924at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="233" mass="24918" checksum="D606AA7A2A30DBF0" modified="2014-01-22" version="1">MNAVPPLVTLHVWRVPRRALGSALLRMALDPRRLRARPGVRFGKLLGTGTGTGFGPGDADLTRYAAVMVWDDPDGGELGPAGAAWDSIAAQSARVDLTPLVSRGSWSEQTPFGDPPGGRAEGTVLALTRARLRPVKALTFWRAVPPVARELAAAPGLLARFGIGEAPIGWQGTVSIWRSAADLTGFAYRQPEHRAVIARTPAARWYAEDLFARFAVQGISGDKAVLGWHEGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTP4</accession>
    <name>U5VTP4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358005.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03855"/>
    <dbReference type="EnsemblBacteria" id="AGZ39060">
        <property type="protein sequence ID" value="AGZ39060"/>
        <property type="gene ID" value="AFR_03855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.779"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_11"/>
    <dbReference type="OrthoDB" id="831053at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="10"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="32175" checksum="F96634E6032D3EC5" modified="2014-01-22" version="1">MRAALLHACGAPPALGDRAAPVPGDDEILVRVTAASITPLDLLCASGTSYFGEPDTPYVPGVQGVGVREDGTAVWFPTSAGMRPGDGSLAEFAAVRAADTVELPEGADHRLVAALGLSAVAAHLALTWRGELQPGEQVLVLGAGGVVGQAAVQLALLAGARRVVAVARSERAQQRAARAGAAVVVPLEEGAEIAHLAARMRAAADGPVDLVLDPLFGVPAAAALRALRPGGRLVNLGSAAGEIAPLDSATLRSGSLRILGYTNNELTPQLRADTLTLIASHAAAGRLTVDFETVRLHDITTAWHRTDCRIVVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTQ4</accession>
    <name>U5VTQ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358015.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03905"/>
    <dbReference type="EnsemblBacteria" id="AGZ39070">
        <property type="protein sequence ID" value="AGZ39070"/>
        <property type="gene ID" value="AFR_03905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.789"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023620_4_1_11"/>
    <dbReference type="OrthoDB" id="1456605at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="145"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="458" mass="49168" checksum="D334909B034A026E" modified="2014-01-22" version="1">MDRLTNVTVVDPRDGTTTAHQDIHFADGRITKIGTTAGPHPDAIDGQDRFVVPGFVDMHAHPLNLADPAPELGLMLAFGITGFRQMSGTPDLLRHRPDTTDRTPALLATPGPVLTPINAGKPATAVAEVQRQARAGADFIKAALITPDVYYAVQAEADRLGIPVVGHLPAGIDVRAASRAGFRSIEHVGPQLGVLAGCSCHEDHLRAASSGNPIPAPPFKIPFADRIAGKLLQRIVINPSQLTTPATLAAVRETIDTFDEDKARTLARQFADNDTWNCPTLIRIKAQQLCDNPTFAADPNLRYATTKERKAWTRAAESFHQKFTEAQRATFTEQFDLQKRMVKILAEEGAPLLAGTDAVGAAWVIAGASLHDEFDLLAESGLTPLRILQTATTDPARFLNREKTAGTVSAGKEANLVLLDADPTTKAANLHTISAVIRAGTHHNKEALQSLKAQATNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTU9</accession>
    <name>U5VTU9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyridoxal-dependent decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_04205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358075.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04205"/>
    <dbReference type="EnsemblBacteria" id="AGZ39130">
        <property type="protein sequence ID" value="AGZ39130"/>
        <property type="gene ID" value="AFR_04205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.849"/>
    <dbReference type="eggNOG" id="COG0076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028929_2_1_11"/>
    <dbReference type="OrthoDB" id="1478871at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="483" mass="49829" checksum="2D3A90CDBEE9475B" modified="2014-01-22" version="1">MIEALPSTGVPAGQVLAELQELRTADLPTHGGRLFAYVYDPDVDGLDELARAAYAISAHVNGLDPTAFPSLLAMENALVTSAARLLGAGPGTTAPDVVGSVTSGGTESLILAVKAARDAHPEITEPRLVVPSTAHAAFAKAAHYLRVALDVVPVGPDLRADSVAMAAAIRPETVLVAASAPSYAHGVVDPITAIAAAAAERGVRFHVDACFGGWVLPYLRRLGAELPEFDFGVPGVTSISVDLHKYAYAPKGVSVLLHRDAGLRMPQFFAYADWPGYTMVNPVISSTRSGGPIAAAVATLRHLGDDGYLRLAGKTRDAVGVLADAVEATDGVRLFAPPETTVVCLTTTDGVDLFVLADELAARGWHTQPQLAYAELPATIHLTVTAAVGATAAEFAKDLAAAVSATRDRGPIDLPPALLEAAGALTPAMVTPELIEGLAAGLGLGDNIAGSMASVNSLLNIAPAPLREALLAGFLSLLQRPTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU84</accession>
    <name>U5VU84_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rplD</name>
        <name evidence="7" type="ORF">AFR_04510</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="function">
        <text evidence="5">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358170.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04510"/>
    <dbReference type="EnsemblBacteria" id="AGZ39191">
        <property type="protein sequence ID" value="AGZ39191"/>
        <property type="gene ID" value="AFR_04510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.911"/>
    <dbReference type="eggNOG" id="COG0088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041575_5_0_11"/>
    <dbReference type="OrthoDB" id="1572673at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_L4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4/L1e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="PTHR10746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="216" mass="23016" checksum="E855649283F8857C" modified="2014-01-22" version="1">MSSVDVINAEGTKAGSVELPDDIFDVQANIPLMHQVVVAQLAAARQGTAKTKTRGEVAGGGKKPYKQKGTGRARQGSIRAPQFTGGGVVHGPVPRDYSQRTPKKMKAAALRGALSDRARDGRVHVVEAFVSGEKPSTKAAIATLRKATESVKVLVVLSSYDELNWISLRNEPTVHLIEAGQLNTYDVLVADEVVFTKEALDEFLGVPAEAAEEGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUJ3</accession>
    <name>U5VUJ3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mycothiol S-conjugate amidase</fullName>
            <ecNumber evidence="2">3.5.1.115</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mca</name>
        <name evidence="3" type="ORF">AFR_05530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">A mycothiol (MSH, N-acetylcysteinyl-glucosaminyl-inositol) S-conjugate amidase, it recycles conjugated MSH to the N-acetyl cysteine conjugate (AcCys S-conjugate, a mercapturic acid) and the MSH precursor. Involved in MSH-dependent detoxification of a number of alkylating agents and antibiotics.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + mycothiol S-conjugate = 1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside + an N-acetyl-L-cysteine-S-conjugate</text>
            <dbReference type="Rhea" id="RHEA:36543"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58718"/>
            <dbReference type="ChEBI" id="CHEBI:58886"/>
            <dbReference type="ChEBI" id="CHEBI:59633"/>
            <dbReference type="EC" id="3.5.1.115"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MshB deacetylase family. Mca subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.115" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358926.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05530"/>
    <dbReference type="EnsemblBacteria" id="AGZ39395">
        <property type="protein sequence ID" value="AGZ39395"/>
        <property type="gene ID" value="AFR_05530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1122"/>
    <dbReference type="eggNOG" id="COG2120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049311_2_2_11"/>
    <dbReference type="OrthoDB" id="1282433at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010126">
        <property type="term" value="P:mycothiol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010127">
        <property type="term" value="P:mycothiol-dependent detoxification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01482">
        <property type="entry name" value="Mca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017811">
        <property type="entry name" value="Mca"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="PTHR12993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF16">
        <property type="entry name" value="PTHR12993:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="SSF102588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03446">
        <property type="entry name" value="mycothiol_Mca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="32823" checksum="F8C2A83E9B5424F1" modified="2014-01-22" version="1">MAEQLRLMAVHAHPDDESSKGAATMARYVAEGVEVLVATCTGGERGSVLNPKMDRPEVLADISNIRRAEMDKAREILGVEQAWLGFVDSGLPEGDPLPPLPEGCFGLVDPAEGALPLIKLIRQFKPHVMTTYDENGGYPHPDHIMCHKISVAAFEAAGDPEQHPELGEPWQPLKLYYNSGWTRARFMALHEAMLAAGLESPYTEWLDKWEDRQDRGDKITTRVECGEYFEVRDDALRAHATQVDPDGFWFHVPMELQQKVWPTEDFELARSFVDSPVPESDLFAGIRESVQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVA6</accession>
    <name>U5VVA6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_12280</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12280"/>
    <dbReference type="EnsemblBacteria" id="AGZ40742">
        <property type="protein sequence ID" value="AGZ40742"/>
        <property type="gene ID" value="AFR_12280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2494"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3899">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="850"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="913" mass="97928" checksum="A866D9E6AFEDDD18" modified="2014-01-22" version="1">MDLVGRRVEREAVEKFLSLARVGRSGSLLVRGEAGIGKTAVLEHARQAALASHFRVESSVGVESETQFAFAGLHQLCAPLLDRTGVLSEPQRDALGVAFGLHGGAAPDRFLVGLAILNLMAEVAEGQPLLCLVDDAQWLDEASAEVLAFVARRVGAERLALVFGLRDSDDADHLPFTGLPELRLERLGASDARALLSAAVRTPLDEGVRERILAEASGNPLALLELPRNIRPADFAGGFELPDVLDVPHRIEEGFRRRSASLPPETQLLLLVAASEPTGDAALLWRAAARLNLVREAAVPAEIAGLLEIGVRVRFRHPLVRSAVYRAATPPDRRRAHGALADSTDPKADPDRRAWQRAQSVLGVDEEAAEGLERSAERARARGGMAAAAAFLQRAAELTPEPALRAQRALDAAHAKHEAGATEAALELLAVAAAGPLDALPRARLDLLLAQIAFYQSRGSDVPGMLLDAAKTLAPLNPALARETYLHAIDAAIHAGRLVHGRGLLEAAEAALEAPAPSTPPQPVDLLLDGLATRFTRGYEVSVGPLRRALETLRDHGSRTGDGDLRWLWLACHVAAMLWDDEALYVLAGRAVRLSREAGALSMLPAALNALASVLVLTGDLGRAAELAAEETAITQATGAPPLPNARLMLAVWRGQQPEALELYSAMVEEATGRGEGATVGLAQATLAALHNGLGNYDEALAAAQPPWEYDDLAYSSLALPELIEAASRAGQPKRAFAALEQLTSRARASGTQFALGLTARSRALLTTGPAAEEHYRQAIERLSDCRMAVYLARTHLVYGEWLRREGRRQDARDQLRTAYKLLSDMGAEAFAARAARELRATGEHPSKRNAEPADALTAHELHIARLVATGLTSREVGAQLFLSPRTIETHLRNIFRKLGITSRRELKELHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-02-10" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVQ8</accession>
    <name>U5VVQ8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_13785</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FPG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13785"/>
    <dbReference type="EnsemblBacteria" id="AGZ41043">
        <property type="protein sequence ID" value="AGZ41043"/>
        <property type="gene ID" value="AFR_13785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2799"/>
    <dbReference type="eggNOG" id="COG0266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038423_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="DNA_glyclase/AP_lyase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="MutM-like_N-ter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="SSF81624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FPG_CAT" evidence="10">
        <location>
            <begin position="4"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ41043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="29279" checksum="CC73A6544B917B79" modified="2014-01-22" version="1">MIMPELPEVESARAVIERGGLRRKIVEVDDSDSYVCRPHLPGQIREALLGRTLVAAHRRGKSMWCDTSDDGPELGVHLGMSGKIVIADPDGTEIDGGDYWEGRRGSGDYRWSRFALTFADGGRLLLVDPRRLGRVRLDPPVESLGPDAATMTPAQFRAALARGTAPVKARLLDQEAVAGIGNLLADQILWSARINPGRPVGELTPDDVNRLLRATRRAVRSAIAGGGVHTLELIPFRFAGGHCPRDGAPMEKSTVGGRTSWWCSREQAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWP9</accession>
    <name>U5VWP9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_08835</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08835"/>
    <dbReference type="EnsemblBacteria" id="AGZ40056">
        <property type="protein sequence ID" value="AGZ40056"/>
        <property type="gene ID" value="AFR_08835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1798"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116199_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16589" checksum="D17055CD8305E8B4" modified="2014-01-22" version="1">MTSDWTATLPRKRMGAGVLFRDGGDRILLVEPTYKPEWELPGGVVEADESPRAAAAREVTEELGLTVVPGRLLAVDWVPARDGRTEGLMVVFDGGVLADPGRIRLQESELRSWAWSTPAEAAARLRGLVARRSEAARRAALTGTTAYLENGHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX49</accession>
    <name>U5VX49_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15835</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361508.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15835"/>
    <dbReference type="EnsemblBacteria" id="AGZ41449">
        <property type="protein sequence ID" value="AGZ41449"/>
        <property type="gene ID" value="AFR_15835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3215"/>
    <dbReference type="eggNOG" id="ENOG5033E29">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175337_0_0_11"/>
    <dbReference type="OrthoDB" id="1829408at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="93" mass="10724" checksum="58477E1330EE269D" modified="2014-01-22" version="1">MQYIHVSWVHDYVDEPVELLSEVDGDGDEVRRVQIFRDGRLEWADGLGGTDVGGLSEVPIDLEEIRRQPGEFLLTVIDRQRFEQEWGRAGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXF3</accession>
    <name>U5VXF3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361531.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15950"/>
    <dbReference type="EnsemblBacteria" id="AGZ41472">
        <property type="protein sequence ID" value="AGZ41472"/>
        <property type="gene ID" value="AFR_15950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3238"/>
    <dbReference type="eggNOG" id="ENOG5032SXW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978858_0_0_11"/>
    <dbReference type="OrthoDB" id="1010556at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="32296" checksum="EF6CAD05A00A4BEB" modified="2014-01-22" version="1">MALGVAPITTTDVPAVARFLHENLNDRVTPEAWERAVQVPWKVSAPNHGFLLRDADGAVVGAYLAFYSERTIDGRTRRFCNLGAWCVLPQHRFHSLKLLKALLAQDGYDFTDLSPSGTVVPMNTRLGFTTLDTATALAPNLPWPAVPGLGRVSTDPVTLENTLTGDDLQIYRDHTGAAATRHVLLTRGDRYCYVIYRTDRRKNLPFFASILYVSEPELFRALQRRFQSHLLVRHGILAMLAELRVVGRRPALSRMLPDTRRKMFRSADLKARDIDYLYSELTCLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXW7</accession>
    <name>U5VXW7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATP/GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_11255</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360504.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11255"/>
    <dbReference type="EnsemblBacteria" id="AGZ40540">
        <property type="protein sequence ID" value="AGZ40540"/>
        <property type="gene ID" value="AFR_11255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2293"/>
    <dbReference type="eggNOG" id="COG2229">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077970_1_0_11"/>
    <dbReference type="OrthoDB" id="1438064at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="21599" checksum="9BBCA4F5D9428570" modified="2014-01-22" version="1">MHYSDDSDLFPTALKLLIAGGFGVGKTTFVGAVSEIEPLRTEEILTTAGVGTDNLTGIERKTTTTVAMDFGRITIDSTHVLYLFGTPGQERFWFMWDELSAGAMGAVVLADTRRLQDCFAAVDFFESRGIGFLIAVNQFDNSFRYEPEDVRSALDLRAEVPIVTCDARDSRSSTGVLVRLIQHLLGANSTPTPVRSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXY9</accession>
    <name>U5VXY9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FMN dependent NADH:quinone oxidoreductase</fullName>
            <ecNumber evidence="6">1.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Azo-dye reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">FMN-dependent NADH-azo compound oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">FMN-dependent NADH-azoreductase</fullName>
            <ecNumber evidence="6">1.7.1.17</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">azoR</name>
        <name evidence="8" type="ORF">AFR_17925</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Also exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 a quinone + H(+) + NADH = 2 a 1,4-benzosemiquinone + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:65952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="ChEBI" id="CHEBI:134225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H(+) + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:55872"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15783"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:71579"/>
            <dbReference type="EC" id="1.7.1.17"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:55874"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="6">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the azoreductase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.-" evidence="6"/>
    <dbReference type="EC" id="1.7.1.17" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17925"/>
    <dbReference type="EnsemblBacteria" id="AGZ41863">
        <property type="protein sequence ID" value="AGZ41863"/>
        <property type="gene ID" value="AFR_17925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3636"/>
    <dbReference type="eggNOG" id="COG1182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088964_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016652">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01216">
        <property type="entry name" value="Azoreductase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003680">
        <property type="entry name" value="Flavodoxin_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023048">
        <property type="entry name" value="NADH:quinone_OxRdtase_FMN_depd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02525">
        <property type="entry name" value="Flavodoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 6" id="KW-0288">FMN</keyword>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin_2" evidence="7">
        <location>
            <begin position="8"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23302" checksum="70851EEAD2D720C6" modified="2014-01-22" version="1">MIWLMTYLLHLDATSFDAESISRSVARSFLDTWPGEVVHRDLAAAPVPHLTAAGISARLTDPARHNPDEAAAAALQDELIDEFLGASAYLFTVPLYNYAAPSSFKAWLDQIVVTGRTHARPDGPPAAGRRALVISSRGGSYGPGAPHHGRDHLVPHLREIMGGELGLELEFITPEFSLAPVVPALAQFLDFHRASLGDAHDQARKLGGSFTPGRKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY41</accession>
    <name>U5VY41_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362291.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18200"/>
    <dbReference type="EnsemblBacteria" id="AGZ41918">
        <property type="protein sequence ID" value="AGZ41918"/>
        <property type="gene ID" value="AFR_18200"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18200"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3694"/>
    <dbReference type="eggNOG" id="COG3211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045986_0_0_11"/>
    <dbReference type="OrthoDB" id="796397at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008557">
        <property type="entry name" value="PhoX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05787">
        <property type="entry name" value="DUF839"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665863" evidence="1">
        <location>
            <begin position="30"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="386" mass="40802" checksum="13CD1B3AD4EE2D50" modified="2014-01-22" version="1">MDRRTLLRATVLGAGAVALPFTSWSAAYAAAQPGAGPYGPLRSADAHGIKLPAGFTSSVVARSGHRVAGTGYKWHDAPDGGATFPDGDGWIYASNSEVPPPAGRGGASMIRFDADGRVTGAKRILAGTTSNCAGGGTPWDTWLSCEEIDRGRVFETFPKGGPAIARPAMGRFKHEAAAADPDRKVVYLTEDEPDGRFYRFVPDRWPDLSAGTLQVFCAGDGSSGAFTWEKVPDPGGAPARTRHQVRAAKPFNGGEGCYYAGGTVWFTTKGDGRIWEVDLGAGRFRLAYDDNLVKPGPAPLTGVDNLTGSSSGDLYVAEDNGSMDICVVTPSGRVSRFLTVTGQEKSELCGVAFNPKGDRLYFSSQRGRSGLITDGITYCVRGPFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ69</accession>
    <name>U5VZ69_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ricin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18830</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="U5VZ69"/>
    <dbReference type="STRING" id="1246995.AFR_18830"/>
    <dbReference type="EnsemblBacteria" id="AGZ42042">
        <property type="protein sequence ID" value="AGZ42042"/>
        <property type="gene ID" value="AFR_18830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3824"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082328_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="3">
        <location>
            <begin position="182"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="30398" checksum="72AA777CAFC4C6A2" modified="2014-01-22" version="1">MADEDLERNDPVLVRPYITTEPGSAPGGHDDRPLETWPEEALLPSEDNSAAPAATVEPPAVAGPAAKTSALLRQRLMVLSGLGILAVLIGAGLLVFGTSEDQPPPQAARPTAPAPGPNGSLGAAPVASVAGRSIAPTSRATSRPAAPGRTTSGAPAAGAPAPQQPGTPTGTPPPAATTLEPPPAQARTGAITAASGRCLVLGGLLALDGSPVQTASCSGMSYQEFTVETDGTLRVTNRCAQTDDDGTVHIRACGEQPAQQWRAGPEGSLVNPGTGQCLTDPGNAGATVTLAACTGADNQRWTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZE6</accession>
    <name>U5VZE6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_13920</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13920"/>
    <dbReference type="EnsemblBacteria" id="AGZ41070">
        <property type="protein sequence ID" value="AGZ41070"/>
        <property type="gene ID" value="AFR_13920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2824"/>
    <dbReference type="eggNOG" id="COG2409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005108_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="558"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="626"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="672"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="238"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ41070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="754" mass="79074" checksum="9D5FA1E109A3E630" modified="2014-01-22" version="1">MSADRLDRPPTGQNRPSVRPMTVDRPVGLPVRGMAIHRRRCLHVPVMVAALGRSCARLRWPILGAWLVLVVAGLVLGGQVFDRLSTAGSLPPDAPSQLAERRVDRLQPEGPVVVAVVGGRDVYDPAVVQAVTAVTGEIRTMDGVAEVEDLYNAAGGRIGADNRRSLIRVELRQGLPDDRREQVEDAVAAALHRIDAPEVLVGGEKLAERAFEDQAIADAALGESVALLILLVVLVVILGGFVAGLIPLVAAVAAVSVTLLALFGLTAFTEVSPFTVNVVTLLGTGLAVDYALLVLARFRQERDADPGAAIAELLGRTSATAGRTVLISGLAVAAAMGGLSVFSEPLLGAMALGGAVAVLLATLAGLTAVPALIAVAHRRIPARVPAVGRSGRGPGLLARLAGFAQARPGPVALAVTAGLLALSLPFVFAVNLENSDARALPASAEARKVQEVVQRDFSGGQADPIVVVVDTDPAGTPVRDFLNAVNTLDGVLRVEPRPDIAGPAAILDVTPEGATADRTSRDVVRAIRALDTPFPVLVGGAAAELVDYRDSVAGRFPLALLVVVLSTLVLLFVLTGSVVLPVKALMMNALTLLATLGVLVVVFQWGVGSALLGFDSWGAIDVTTPVLLFVFVFGLSMDYEVFLLARIKEERDPRDPTGDRAVRAGLEKSGPVVTAAALCIGVVFLGFLLGDLVAVKEIGFAMTVALLLDVTVVRGLLLPALMKLLGEWNWWAPAPLRRLHERLFVQPDDSERMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W001</accession>
    <name>U5W001_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_21330</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21330"/>
    <dbReference type="EnsemblBacteria" id="AGZ42538">
        <property type="protein sequence ID" value="AGZ42538"/>
        <property type="gene ID" value="AFR_21330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4327"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_43_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="4">
        <location>
            <begin position="22"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="TetR_C_33" evidence="5">
        <location>
            <begin position="92"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="20374" checksum="340190C907617D6F" modified="2014-01-22" version="1">MRSCPSWPASVPRAGLDAATVVAAGAALADEIGLSELTMGRLAERVGVRAPSLYKHVTGQDDLNRRIAALALTEAADAVGAATQGRAGRDALAAAARALRDFVVTHPGRYAATIGLTAGGTDDPLAVGLEPFAAVLRGYDITPQEMTHALRALRSIFHGFATLEAGGGFRWPDDIDDSFSWLVDLVDRGLSSPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W074</accession>
    <name>U5W074_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alpha-1,2-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_15490</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15490"/>
    <dbReference type="EnsemblBacteria" id="AGZ41380">
        <property type="protein sequence ID" value="AGZ41380"/>
        <property type="gene ID" value="AFR_15490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3145"/>
    <dbReference type="eggNOG" id="COG3537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003690_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005887">
        <property type="entry name" value="Alpha_mannosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041371">
        <property type="entry name" value="GH92_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012939">
        <property type="entry name" value="Glyco_hydro_92"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07971">
        <property type="entry name" value="Glyco_hydro_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17678">
        <property type="entry name" value="Glyco_hydro_92N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01180">
        <property type="entry name" value="aman2_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665924" evidence="2">
        <location>
            <begin position="19"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_92N" evidence="4">
        <location>
            <begin position="25"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_92" evidence="3">
        <location>
            <begin position="257"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="957" mass="100795" checksum="B1203B1EB7FEF5E9" modified="2014-01-22" version="1">MAALVAAILLVPATPAAAADAPVDLVRPFVGTQNFGNTFPGAAAPFGMVQVSPDTGGQGGYDYLQNTIYGFSQTHLSGVGCGVAGEVPIMPTTGAVSSVDPTVYRSAYSHTDEQATPGYYRVGLSKYGVRAELTATPRTGWQRYTFPSTNAANVLFNTGKANQGVLDSEIHVVGDRTVEGRVKAGGFCAGRDDHTVWFSASFDRPFASYGTWRGTTRSPGTRDASGTGGNGGWVTFDTTGDRDVVAKVALSYTGADGARRNLTAETGTSYDFDATRTTLRTAWAQQLDAVRITGGTADRQSAFYTSLYHSLMHPNLAGDVDGKYMGFDDRVHTADGYTPYQNFSLWDTYRPQNQLLEMLVPQVARDVARSVVAVGRDGGWLPRWALANSETNIMTGDPVTPFLVEAFSKGLLAGLEEETYTLLRANATSQPPADSQYNGRTGVGYYVDRGYVPSGLTVGTDCRHKGGDNDCVHPASSTLEYAAADASLALMAGALGKTADARMFTARGQSYRALWDPSIKHFRPRTTAGAFVTPYDPVEAGHQFHEGGAYQYQWLVPQDPAGLVGLMGGRAGAEKRLDDFFAYNQLLTDPAGTARSAWITAPYDYYGKATYNPNNEPDLLAPYMYHWAGAPAKSATVVRAAMTLFTTGPDGMTGNDDLGTMSAWYVLSSLGIYPTMSGAGFLALSSPQFPSATVRIGAYADRQGGTLTITAPDVTDSNRYVRQVRLNGADHTRNWLDWSAIARGGTLDFTVGTTVSAWGTAVADQPPSVSAAPVLPCAATTGGQCAVDLAAVRTTDGTATTAANAEGNFDGAGWSYDAALLPAAGTVSWGGITYSTPAPGGAAPNFVRARGQEIRVPAGNRQSVRLVAASHHGPVPGTVVVTYADGSSASTALTVTDWCGSGPAVLTMPNRIKAGQGVDGPAASLFGLTVPADPAKHIRSVTLPGDPRILVYALTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0B7</accession>
    <name>U5W0B7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CMD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361489.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15740"/>
    <dbReference type="EnsemblBacteria" id="AGZ41430">
        <property type="protein sequence ID" value="AGZ41430"/>
        <property type="gene ID" value="AFR_15740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3196"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_3_2_11"/>
    <dbReference type="OrthoDB" id="1925754at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="54"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="187" mass="20266" checksum="7FF7EAD14BDD44F2" modified="2014-01-22" version="1">MRIDDVDPADMSAAQRELYDIYTTGRRVAPESPFSLVGADGRLLGPPAVWVISPAFGKALQHIGSAVRYDSRLPVRAREIAILLVGHHHRSAFELYAHERAGAAAGLTAEDLAALAETREPSGLSEVEACVFRTTTRIVDRGTLDGDEYKDAEGVLGAEGLFELVTIVGYYNMVAWQLAVFDIQPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0E9</accession>
    <name>U5W0E9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15620"/>
    <dbReference type="EnsemblBacteria" id="AGZ41406">
        <property type="protein sequence ID" value="AGZ41406"/>
        <property type="gene ID" value="AFR_15620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3172"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_257666_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010496">
        <property type="entry name" value="3-keto-disaccharide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029010">
        <property type="entry name" value="ThuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06439">
        <property type="entry name" value="DUF1080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06283">
        <property type="entry name" value="ThuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F5/8 type C" evidence="1">
        <location>
            <begin position="125"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="2">
        <location>
            <begin position="909"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1290" mass="138455" checksum="2458E31C1334AF7A" modified="2014-01-22" version="1">MLLIAGTPAIAHAAPAAAVPMEVLVFHGPAAEQQDPVARATQAVKELGQADGFTVTETTDPATFTPAKLAKYRAVVFLSATGAALSRDQETALQSYVKAGNGFVGIGDAASAQLDSAWFTGLIGTRPAGSIPVPEAVSKVTASGENTPNETKEKLTDGDANTKWLVRTPAAWVAYELSKPTAITGYALTSANDFPGRDPKDWTLQGSADGTTWTDVDKRTGQTFADRFLIRKFEVTGAAEFKFWRLNITANSGEPLIQLADLRLFTGTMSAPQPPAVNEAVVDILDRNHPSTKALPTTVKRSDRWYNWDPNPVGTVHTVAQVEERFYDPGPGANGPFHPVSWCRDYEGGRSFYTGMGHTEGSYGENAFRGHLAGALRWTTGVERGDCQATIAANYKVERLTAANKTGQLDQIGEPHGLTIADDGTVFYVGKAACPTGPVVDWANPNVGLGCGTIHSYDPASKQVKLLTTLPVMGNRGSGSELVKNEEGLLGIVPDPAFATNGWLYVYWMPHDSIDREKRIGQRTVSRLTYDKATQTIGQASRKDLLKFPVQIHSCCHAGGGMAFDNKGNLYVGSGDNNSSEGSSGYSGNNWTAAYEGVSFQDARRTSGNTNDLAGKIIRIHPEADGTYTIPAGNLFPPGTEKTRPEIYVMGVRNIARLAIDPEHQWLTAGWVGPDAAAPSPTLGPAKYESATIITSAGNQGWPYCMGNKQPYRDRSSTDATVLTGWYDCDNLKNESPRNTGLVDVPPARDNMIWYSPDGGGPVFPDRADGSGIPTYNKADATYTQPYLRGGGQAIMSGPTYHRDRVDTASGVAWPAYWDEKWFIGDQSNAANRVAVTVDPAGVPQQAPPIFGESLRSIIPSGNGDTRLQSWMDAKFGPDGALYLLDYGGGFFSLHANQKLIRITYTGGAPTPAPAASSVAVQNKPLTIAFNGTRSGGVSYRWEFGDGGTSTEANPRHTYARTGKYTAKLTITYANGETATVQTAVTAGCTTPDTRSTVFVDDVNTGVENKQAGEGCTVNDLIDDESTWPDHDSFVRHVTAVTRTLQGDGVLTGREAGTLTRAAAGSDVGKAGHRGYEPLFDGTAESLLGWEQAPSGQFTIQPDGSLRSGGGLGMLWYTKQYADFSLKLQFRDVAPGTARANSGVFTRFPDPRIPLDERPPGSCGTTGSARTSEAWVAIFCGHEIQLYDGDTGETQKTGSIYNFDPVTLPDARVTPKNVWNSYEIRVVGQHYTILRNGVVINEFDNTPGKQSSRAGDPPTDLRQFASGFIGLQNHSDTDLMDFQNIRVRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0F1</accession>
    <name>U5W0F1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DegT/DnrJ/EryC1/StrS aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361524.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15915"/>
    <dbReference type="EnsemblBacteria" id="AGZ41465">
        <property type="protein sequence ID" value="AGZ41465"/>
        <property type="gene ID" value="AFR_15915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3231"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_6_0_11"/>
    <dbReference type="OrthoDB" id="1006364at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="366" mass="37918" checksum="C9EF2718FDECB872" modified="2014-01-22" version="1">MTRIPLVDLAAAHAEVAEEVETGFKRIIAETAFIGGAEVAAFEQEYAAFSGLPHCIGVANGTDALELALRAVGVGPGTEVILPANTFIATAEAVARAGARVVLVDCDPATYLIDVEAALAAVTPATRAIAPVHLYGQLAPVEQLRAGLAGRDVAIVEDAAQSQGATRHGVAAGADGIAATSFYPGKNLGAYGDAGAVVTASEELATTVRTLGSHGGLKKYTHDLIGVNSRLDGLQAVVLRAKLTRLAGWNDRRRAAAARYDELLAGLDVVRPVTLDGNEHVWHIYCIRVPGGRRDAVVAELNAAGVGAAIHYPVPVHRTGAFEHLGGSFPNAESAAPEIMSLPIYPQITPDQQTRVVETLTAAIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0G7</accession>
    <name>U5W0G7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-dehydro-3-deoxyglucarate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_15720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15720"/>
    <dbReference type="EnsemblBacteria" id="AGZ41426">
        <property type="protein sequence ID" value="AGZ41426"/>
        <property type="gene ID" value="AFR_15720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3192"/>
    <dbReference type="eggNOG" id="COG3836">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059964_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH_HpaI" evidence="1">
        <location>
            <begin position="21"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="263" mass="27930" checksum="34770AE83F3548C8" modified="2014-01-22" version="1">MTRMGFARRLATTDRCLVGTWVKIPALEPVEILADAGFDYIVIDQEHAPLTLEFAYQATVVAQGAGMSVLVRVPDRSGSHLQRLLDSGVDGILVPRVTSVAEATASVRQMVFSPAGDRGLGTTSRAGRWGGTPRAEYLRFGDEEVMRAVQLEERAAIETVEDILDVPGLNGVFLGMGDLGLSSGLTQADPEFQKLVDRFLTAAHDRGVPAGTAVQTAAQAQEAADRGYRYVMVSNDTSLLRGAATSVVAGLGSRFTEGAPHGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0X6</accession>
    <name>U5W0X6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA polymerase sigma factor rpoD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_16635</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361684.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16635"/>
    <dbReference type="EnsemblBacteria" id="AGZ41605">
        <property type="protein sequence ID" value="AGZ41605"/>
        <property type="gene ID" value="AFR_16635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3373"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_5_11"/>
    <dbReference type="OrthoDB" id="1857862at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014322">
        <property type="entry name" value="RNA_pol_sigma-B/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02980">
        <property type="entry name" value="SigBFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="7">
        <location>
            <begin position="54"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r3" evidence="6">
        <location>
            <begin position="136"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="8">
        <location>
            <begin position="218"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ41605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="272" mass="30288" checksum="3A4B5C58462F3B46" modified="2014-01-22" version="1">MNASVADACRTHQPRRSGQFPPTDADKRANEMVIALAAMAPDDPRRAALRRRTIEAWLPMAQRLGRRYAGRGENLDDLQQVAMVGLIKSVDRFDAERGDDFVGFAVPTILGELRRHFRDRTWSMRVPRKVQEMRLAINEANATLFHKLNRLPTVADISQHLGVSEEDVLEGLEGGRAYRATSLSTPVAADATVVLADTLAEEEHGYDLVEIQLALGPALARLTARERQILAMRFWGNQTQSVIAEKVGVSQMHISRILSTTLAKLRTELTVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1R8</accession>
    <name>U5W1R8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1775 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24485</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24485"/>
    <dbReference type="EnsemblBacteria" id="AGZ43163">
        <property type="protein sequence ID" value="AGZ43163"/>
        <property type="gene ID" value="AFR_24485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4959"/>
    <dbReference type="eggNOG" id="COG4549">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087540_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012533">
        <property type="entry name" value="YcnI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038507">
        <property type="entry name" value="YcnI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07987">
        <property type="entry name" value="DUF1775"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665981" description="DUF1775 domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1775" evidence="4">
        <location>
            <begin position="92"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="23117" checksum="55B07CD146326508" modified="2014-01-22" version="1">MKRLTTIGVLAGLGVLVLAGPAAAHVEVSADKTRAGATDVTLTFEGEAENPSGIKSERVVLPEGIAPADVTLVKAPKGWTFAATADGFTVGGKALASGQDAEWKVKVAKFPDGEQRLSFKTVETYADGEVSRWIEIQKEGADEPENPAPLVTLKPGPAPTSAAPAPSSAAPASAAPVASPPAATIQAEPAASTDDGSSTWWIWVLVALAVVGGGTFLVLRRRSKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Z2</accession>
    <name>U5W1Z2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GSDH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362319.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18340"/>
    <dbReference type="EnsemblBacteria" id="AGZ41946">
        <property type="protein sequence ID" value="AGZ41946"/>
        <property type="gene ID" value="AFR_18340"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18340"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3722"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012344_1_0_11"/>
    <dbReference type="OrthoDB" id="152835at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666005" description="GSDH domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="GSDH" evidence="2">
        <location>
            <begin position="126"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="689" mass="75383" checksum="D64F81A1B937D2F8" modified="2014-01-22" version="1">MMIRRLLLPIVLVLSTLVGIPSAARAADGAVYDPVPTEPETSRLALVLSEYASFPQSVTDPPLVDQRLNRVARINTIMEVPDGSGRRAVPDLNGNLYLVKNGVPHVYLDVAASFQPEFFSGRGLGQGFGYVTFHPDFRRNGLFYTIHTEEAAITAEVPDYRQATVLYHGVITEWKATDPAADTFAGTHREVLRIGFGGQIHGIQEINFNPTAKRGSADYGNLYLAVGDGGQGAGNTDPQNLALPHGKLLRIDPRGTNAPNGKYGIPPSNPFVGQAGKLGEVYAYGFRDPHRFSWDRATGRLYLGHIGEHAVEAIYEVRAGDNFGWSEREGRYVFDKTPTTPCDRLFPLPADDAGYQYPVAEYDHNPAPDWNCTSDVGVAVAGGFVYRGQDVPALRGKYVFGDLVNGWVFYTEADEMRRGHAPATIHRLNLFDDAGVPVRLRDLSSPGAPGDPNRVDLRFGTDAQGELYLLAKANAKVWKVTGTRTFASAPAGTTKVRDTMRPTNWAPVTPSKWKFTGSQVILAEAGVARPGPRRPFEYAVLTAGPVLSSVEIKASVRLDEPVEITNRDVIIVFGYKSDTEFYYAHLSTDNTIYPHNGIFKVNNADRERIDYEWNGRSRGANPAVVDADWHDVKVKHLPATGEIAVYVDGAKDPLMTARDTTFSSGRVGFGSFDNIGRLRDLEVTGTPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W276</accession>
    <name>U5W276_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Inner membrane ABC transporter permease protein YjfF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_18955</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362438.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18955"/>
    <dbReference type="EnsemblBacteria" id="AGZ42065">
        <property type="protein sequence ID" value="AGZ42065"/>
        <property type="gene ID" value="AFR_18955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3850"/>
    <dbReference type="eggNOG" id="COG1172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028880_3_3_11"/>
    <dbReference type="OrthoDB" id="1254678at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="337" mass="35750" checksum="8DA197D3F2FCA90A" modified="2014-01-22" version="1">MIDVSAPPQTTTAPATPRRRRRADRRHIPVLATLALLVTMYAIGVANYDNFSDTQVLLNVFVDNAFLLVVAAGMTFVILTGGIDLSVGAVVALTTVVAATLLNAGWPAIVVLPLVLLMGTTLGLGMGAVIHYFKVEPFIATLAGMFLARGLSLLINRNSIPITDPFWTGMAQERIRFGPGIFISTSVVIALVVVLIGAYVLAYTRFGRTVYAIGGNADSALLMGLPVGRTRIAVYTISGFCSALGGVLLSFYMLSGYSLHAQGMELDAIAAVVIGGVILTGGSGYLFGTVLGVLVLGLIQTIITFQGTLSSWWTKIVIGILLFAFIVIQRLVTRRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2A4</accession>
    <name>U5W2A4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363732.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24380"/>
    <dbReference type="EnsemblBacteria" id="AGZ43142">
        <property type="protein sequence ID" value="AGZ43142"/>
        <property type="gene ID" value="AFR_24380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4939"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_5_2_11"/>
    <dbReference type="OrthoDB" id="1425840at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="6"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="146"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="303" mass="31300" checksum="B58697BD5E9FBB35" modified="2014-01-22" version="1">MKTRFLVLALLWGSSFTLIKEALAGLSPAQLVLARLVLGAAVLLTVAAVTRAGLPRERVVWGHLAAAALFGNVIPFLLLSYGERSAGAGIAGVLVGATPLLTLTVAAFALADERATPRKLTGLLIGFAGLVVVVAPWREALGSIGGEVACFGAAVSYALGFVYVRRFLSPRHLKPVSLAAGQVSVAVLLQAAVSPFLEWGPVTVTPRIVVSIVLLGALSTGLAYVLYFRLIDEVGATTASAVNYVVPIAAVLVSAVVLGEAVTWNVIAGGLIVLAGMAYAENRLRRRTQAASGPSPAQSRGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2S5</accession>
    <name>U5W2S5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endoglucanase</fullName>
            <ecNumber evidence="6">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_25825</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25825"/>
    <dbReference type="EnsemblBacteria" id="AGZ43429">
        <property type="protein sequence ID" value="AGZ43429"/>
        <property type="gene ID" value="AFR_25825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5235"/>
    <dbReference type="eggNOG" id="COG5297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006010_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02850">
        <property type="entry name" value="E_set_Cellulase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004197">
        <property type="entry name" value="Cellulase_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003305">
        <property type="entry name" value="CenC_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02018">
        <property type="entry name" value="CBM_4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02927">
        <property type="entry name" value="CelD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="3 5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5 6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005147817" description="Endoglucanase" evidence="6">
        <location>
            <begin position="27"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="CBM-cenC" evidence="7">
        <location>
            <begin position="30"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="CelD_N" evidence="8">
        <location>
            <begin position="179"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="710"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="739" mass="79199" checksum="F7FF4CBA0AD8212A" modified="2014-01-22" version="1">MIRALGATALLAVALTAIGPASPAAADEVEQLVNGGFTSGTEPWWSTAGMPITLRDGKACVDVPGGTVNRWDAAVGQNDIDLVEGETYRYSFTASGDPAGHVARAIVGLSVAPYDTYFEASPVLSVAGDTYSYTFTSAVTTAQAQVALQVGGSADPWGLCLDDVSLVGGVPPEVYQPDTGPRVRVNQVAYLPRGPKKATLVTETTTKLPWQLKNAAGTTVARGHTVPRGVDVSSGQNVQTIDFSRYRKHGTGFTLVADGETSRPFDIDAAAYEQLRIDALKFYYTQRSGIEIKESLRPGYARPAGHVDVAPNLGDGAVPCQSGVCDYKLDVTGGWYDAGDHGKYVVNGGISTWELLNQFERTSEPARLRDGTLAIPESGNRVPDILDEVRWELDFLLKMQVPAGQPLAGMVHHKIHDANWTGLPLMPHLDPQQRELHPVSTAATLNLAATAAQAARIYKRYDPAFARRALAAARTAYTAALAEPARYAPESDGVGGGAYNDSKVDDDFYWAAAELYLTTGDKKYADAILASPVHTADIFGPGAFDWASTAVAGRLDLALIPNKLPGRSAIRDSIVRGADKYLAIQAGHAYGVPYAPANNMWDWGSNSTILNNLVVVASAYDLTHRDKYRDGVVEGMDYLFGRNALNYSYVTGYGEVSSQNEHSRWYAHQLDPSLPNPPKGSLAGGANSSIQDPVAQSKLAGCVGQFCYIDDIQSWSTNELTINWNSALAWVASFVADQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3K1</accession>
    <name>U5W3K1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_27685</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560136.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27685"/>
    <dbReference type="EnsemblBacteria" id="AGZ43798">
        <property type="protein sequence ID" value="AGZ43798"/>
        <property type="gene ID" value="AFR_27685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5611"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="23883" checksum="44B5EEEBC0DA9986" modified="2014-01-22" version="1">MTSVLAVHDVSKVYGGTVTALDHVTLEIAAGELLAIVGPSGSGKSTLLHIMGTLDRPTSGTVTLGGQDAARLRDRRLSALRGQQLGFVFQQFHLTDGLSAAENVALGLQYAGVPRRRRRPLAAAALDRVGLAHRTQHQPHQLSGGERQRVAIARALVNGPSLVLADEPTGALDSRTGRSVLDLLTRLNAEGTTIAVITHDRDIAAALPRRVELRDGRLVRDTGGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3S1</accession>
    <name>U5W3S1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560122.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43784">
        <property type="protein sequence ID" value="AGZ43784"/>
        <property type="gene ID" value="AFR_27615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5597"/>
    <dbReference type="HOGENOM" id="CLU_1197737_0_0_11"/>
    <dbReference type="OrthoDB" id="2008873at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="21940" checksum="EEBA2EBA48213021" modified="2014-01-22" version="1">MTAVPHHYDTAPLAHTDVLVLDYLAALWAASEELEPGLRDELMTTVADYIAMRRTSPANPLEDAGEILQRLGPPEALAAAARRGRVPAHLRRPAVPVPMAPAAVRVAPSGHLEYAGIGLLTAGSVILPVICPLAGLLLVSGSPRWSPAQKTAAWVLTALPALLGFVMVLGAMAIGGGAEILVLAYLAMVAGSVIAGLSLLPGLTARREHRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W457</accession>
    <name>U5W457_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Group 1 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_22475</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363158.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22475"/>
    <dbReference type="EnsemblBacteria" id="AGZ42765">
        <property type="protein sequence ID" value="AGZ42765"/>
        <property type="gene ID" value="AFR_22475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4556"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1413">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_370368_0_0_11"/>
    <dbReference type="OrthoDB" id="149595at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="243"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="452"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="652" mass="67507" checksum="41195BBCF3DBC856" modified="2014-01-22" version="1">MRLTSSAVPQSSILAEIVGDNRLTDPAVLIRAAGGTDDLTAIAAVHALSAADPEISGPVLAGLLSSPRAVLREHASWALMSLPAREDALDDLVAAVVAGGFGGMLAQRTLAGWGTGADALITAWHDAPDAEVRARIVETLGITAGAVAARAVDAVASDADEDPRVRIAAVAALGDRTGDPTALEIISGLISEDGELGAVARLAAADLAGDAAPVRDQGLTVAQLFLHADLDPQLSRAGAGDTGGIATLLVRLGGALVAERGIGRVLTMSRGTAESALTALRPFEGHQLAAVPLLRPAGSAAEAWPAWAAARRGIRRLLRAHSVDLLHLRMADVGSLAAAEAASELGIPIVFTLAPDPHGAIEFLPREDFGAADQREHLFFRARLVRRLTADAAHVALFPRARLHDDLLRLTGTDIAADPDRFTVVPEGIDLTVTEAAAGATVALDLPPARRGLPLALSVGRLHRVKGMATLVEVWASDPGLWQRCNLVILGGDLANPSADERGQLDLIADVLRRHPEAANGLVMPGHQQHDVVARWLASAGPGSVYVCASLKEEFGLALLEALAAGLVVVGPDAGGPPAYVDQGRTGLLTDTTRPAELARAFRGALDLAAGEGQAARAAFARERVRAGFTVQAMAATLGKIYTTVTKEVDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W485</accession>
    <name>U5W485_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase class C-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560169.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27855"/>
    <dbReference type="EnsemblBacteria" id="AGZ43832">
        <property type="protein sequence ID" value="AGZ43832"/>
        <property type="gene ID" value="AFR_27855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5645"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030169_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="462" mass="50515" checksum="1EA39B9933DDCE1C" modified="2014-01-22" version="1">MLLPRSTPAASGVSSRAITALLDRYEAQGIECHSIMVVRHGHVVAEGWWTPYSAERPHLLYSLTKSFTSIAVGLVIADGLLSLHDRVVDVLYDHVPADASEQARRLTVHHLLSMTTGHVTDSLDEAWEREPDDLIRGFLRVPFPEPEGTLHAYDNATTFVLARMVERVTEQDLPQLLRERLFGPMGIDHAEWDLVASGSSFGFHGLHLTTEAVAAFGELLLHGGRWGDRQLVPRDWVELATRRHVSIPDKNDPGFGYGYQFWTSQHGFYGDGAFGQQCVVVPSHDLVVVVTAAITQEGVGTAQFCWETLLPGLDDVDSARDDQLLAERLRGLSLTPVPGSAGPGRSLTAKIEGSVVPSGSVLTVDPDEHGWLLNLGSGIEVGVGHGEWRESSPLGRPVVATGAWQGDTFVAELYVITSPHRVRVEARPGTPTATVTWSSVPLTSPELRLHLRSPLKTRPHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4I3</accession>
    <name>U5W4I3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363337.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22830"/>
    <dbReference type="EnsemblBacteria" id="AGZ42836">
        <property type="protein sequence ID" value="AGZ42836"/>
        <property type="gene ID" value="AFR_22830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4627"/>
    <dbReference type="eggNOG" id="ENOG50302YB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052229_0_0_11"/>
    <dbReference type="OrthoDB" id="2179463at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="264" mass="28476" checksum="45C536E6A338FD11" modified="2014-01-22" version="1">MVNELRPVDRAVLAVLGRLLPAAFRERQQGEWASDLLTLPADTRLRYLTGAARTLPSLRAQVRRGHVVPELEVGPGLAAGFARIVFFGLAWPILSWLLWVPFRYYAFSVDDRLAAATDPEYTIDPQSVWPFDVTPGWLMIVWWPPHLGAWMAVLSGPFLIVAVTVFGSLLALLGRRARRRVVLLAAVIVGVVGAAATGVLVALEISDNGFAAGFLGAVALGLTVVKGLSTRLRTGLVVVGLGAIALFVAFHTGEGQAMLGWFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4L7</accession>
    <name>U5W4L7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oxidoreductase FAD-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_29435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29435"/>
    <dbReference type="EnsemblBacteria" id="AGZ44148">
        <property type="protein sequence ID" value="AGZ44148"/>
        <property type="gene ID" value="AFR_29435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5965"/>
    <dbReference type="eggNOG" id="COG1018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_380774_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="386"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="5">
        <location>
            <begin position="633"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="723" mass="76792" checksum="858EA189E0A2F03E" modified="2014-01-22" version="1">MVSWENKAVRQFGGPNRLATVDEVEAIVGRPAPVVMLKQISALDEGCRAILANCAVAAFGYRDAAGTSRTTFIGGTPGFARVHSPTRISFRLPESGDPLGPVSLFFLLPGVGEILRVNGTVAARKSGETTVDIDEAYIHCAQAVLRSRLWQPPAPAATPVEITGDGPLRGPGIAEFLAASPFLALSSWDSSGGSDTSPRGDSGAVVRVLDSHTLVIPDRRGNKRADTLHNLLQDDRLSFAALVPGRSGVLHVRGRGAITNDPALLETMALRGMSPHAALLIDVEHAEVTVSNPVARSRMWTPASHPDRGAVPDLMALAGEHLAAGTTRAKGGPPAFLLKAIGAVPGMARLLRLVMNRAYLSGLRKEGYDDVAAGVSRPAAVDRPAQPERPLRIVEVRRETPSVVTLVLQDAGDGRGSFDFRPGQSFTLVTDIDGHPVRRAYSASSAPGSSRLEVTVKQLDGGRFSTHVHSHLRAGDLLTVLGPSGSFHAQPQPPEHVVMVAAGSGVTPMMSMIRAQLAARPAQGRITLLYSSRSPQEIIFFDELTRLERDNPDRLTVTHVLTGRDGRLDADRLHHWITGLPVSRDTHYYVCGPEALTDAVQAVLNGLGVPDTAVHHERYTSAADIRTTTTAPQEMTVHQDGHLIGSAVVEPGQTLLEAGLAAGLPMPYSCTVGSCGDCMVQLRSGEVTRNEPNCLTAQQKADGYVLTCVGCPLSTVVLDVNPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4V4</accession>
    <name>U5W4V4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363620.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23810"/>
    <dbReference type="EnsemblBacteria" id="AGZ43030">
        <property type="protein sequence ID" value="AGZ43030"/>
        <property type="gene ID" value="AFR_23810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4823"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082427_0_0_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="228" mass="24365" checksum="B02F2C08B6DA4672" modified="2014-01-22" version="1">MVEVRQQTRDKIVESAAQLLREHGAAAVTTRAVAQAAGMQAPTIYRFFEDKEALLDAVAEHVFATYVSGKTYAGTDPDPLADMKAGWDTHIDFGLANAALFGLLIDPSRGARSPAVAAGTEILRARVRRVAAVGRLRVPEKRAVDLIHAAGTGAVLALLEMPPEQRDLGLTDAMYTAVMRSILTDVPALPADDATAAAIAFRAVVPELKNLTPVERSLLSEWLDRAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Y6</accession>
    <name>U5W4Y6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29665"/>
    <dbReference type="EnsemblBacteria" id="AGZ44194">
        <property type="protein sequence ID" value="AGZ44194"/>
        <property type="gene ID" value="AFR_29665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6013"/>
    <dbReference type="eggNOG" id="ENOG502Z954">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033907_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="24583" checksum="E4D17D6EF510C817" modified="2014-01-22" version="1">MWSGPRNISTAMMRSFGSRADTVVVDEPFYAHYLAVTGLDHPGRDAVLASQPNRWQDVAASLTGPVSGDVFYQKHMAHHLLPEMGRSWLGSLTQAYLIRDPAHVVASYARVRGEPTLEDLGYPQQVEIFRAYGGPVVDAADVLRDPAGVLGRLCAALGIGFDPAMLHWAAGPRATDGVWAPHWYATVEASTGFAPYNPAPPVVPARLRPLVEAAQPYYDELAAHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Y9</accession>
    <name>U5W4Y9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29005"/>
    <dbReference type="EnsemblBacteria" id="AGZ44062">
        <property type="protein sequence ID" value="AGZ44062"/>
        <property type="gene ID" value="AFR_29005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5877"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_2_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="40"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="27766" checksum="E138ACD669EDD52A" modified="2014-01-22" version="1">MAAVFDQVAGVYDNVGVPWFTPIAEELVRLAAPTNGERALDAGCGRGAATAALARAVGPGGHVTGVDLSPRMAEMCAADIAALGLTNVDVQVLDASSPSLPAAAYDLVVASLVVFFLPDPLAALTAWKRLLVPGGRLAISTFGGRDQVWETVDATFQPYLPAGMLDARTTGAAGPFASDAGVEELVAAAGYTGVRTSHVDIEVTFADVDEWYTFSRSHGQRAMWDAIAATDHNTVKATAADHLEQARGADGRIHLHQQVRSTLATAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5J1</accession>
    <name>U5W5J1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24600"/>
    <dbReference type="EnsemblBacteria" id="AGZ43186">
        <property type="protein sequence ID" value="AGZ43186"/>
        <property type="gene ID" value="AFR_24600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4984"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="143"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="219" mass="23269" checksum="E4EC4F2D506AE21D" modified="2014-01-22" version="1">MLIADDQELVRAGFSMIIDSRDDLEVVGEAGDGVQAVALAGQLHPDVILMDVRMPRMDGIEAARQLAATGHPARIIMLTTFDLDEPVFAALRAGASGFLLKDTRPDDLAAAIRVVAAGEALLAPTVTRRLLDRFAPELPGGSPPPSLEPLSTREIEVLKLVARALSNDEIAERLTLSRATVKTHLSAILFKLGLRDRVQAAVLAYETGLVRPGTAPPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5P1</accession>
    <name>U5W5P1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24875"/>
    <dbReference type="EnsemblBacteria" id="AGZ43241">
        <property type="protein sequence ID" value="AGZ43241"/>
        <property type="gene ID" value="AFR_24875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5039"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008901_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="494" mass="52822" checksum="1EEE7B22ABEB3AB4" modified="2014-01-22" version="1">MTATVDESAAPGGYAETAAPGTDRAIDPERLALCLAVIAEAAELDPEHPDAVSVRRATAGLFKEVKLRRRKDRRDAILANDRAVTAATATGAPSRIDDETAGILLETATKAPTAGVLRQPRGCYICKQRYVQVDAFYHQLCPSCAVKNHGHRDARTDLTGRRALLTGGRAKIGMYIALRLLRDGAHTTITTRFPNDAVRRFAAMPDSADWLHRLRVVGIDLRDPAQVVALADSVAAHGPLDILINNAAQTVRRSPGSYSALVAAESAPLPDGPLPELEYLGGHSSPAHPAALTDGSSFSPHALTALALTGGSATPERIAALTAIDAGGLVPDLAPTNSWSDKVHEVDALELLEVQLCNMTAPFILVSKLRAAMTASPFPRRYVVNVSAMEGVFARGYKGAGHPHTNMAKAALNMLTRTSAEDMFTDGILMTSVDTGWITDERPHPTKMRLAEEGFHAPLDLVDGAARVYDPIVRGEQGEDLYGIFLKDYAPIAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Q3</accession>
    <name>U5W5Q3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25270"/>
    <dbReference type="EnsemblBacteria" id="AGZ43320">
        <property type="protein sequence ID" value="AGZ43320"/>
        <property type="gene ID" value="AFR_25270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5121"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052304_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="6"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="145"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="439" mass="48330" checksum="89030FDFCE4E03FC" modified="2014-01-22" version="1">MSERRRYAVAGAGARAEMFVRALTLDHAGTAELVAFADVNPARMAAHNRWLTELGARPVPAYAAAEFTAMLAKERVDEVLVTSMDSTHAHYVVAALDAGCDVITEKPMTVDADGCRRILDAVERTGRRVSVAFNYRFNPLHEAVREVLASGEIGEIGSVHFEWLLDVRHGADYFRRWHRDKANSGGLLVHKSGHHFDLVNWWLGAAPERVYADGRLFFYGESGRRHGYARDYLRAHDDPAAEGDPFALRLRDNPRMKELYLDAEAEDGYRRDVNVFAPGVTIEDDLAVLVRYDTGATMTYHLTAYAPWEGYRVMVNGSRGRLELEVVESDHVSPAGANEVKGAAIHGVTEAQEKGHASLTVHPFWAPPRELPLPAAYSRTGHGGADTRMTAVLFGAEKDPMHRSATARDGTLALLTGLAANRSLETGLPVRVADLLDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>U5W624</accession>
    <name>U5W624_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidine kinase</fullName>
            <ecNumber evidence="1">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_25685</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.13.3" evidence="1"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364067.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25685"/>
    <dbReference type="EnsemblBacteria" id="AGZ43401">
        <property type="protein sequence ID" value="AGZ43401"/>
        <property type="gene ID" value="AFR_25685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5206"/>
    <dbReference type="eggNOG" id="COG0643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000650_2_1_11"/>
    <dbReference type="OrthoDB" id="305000at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004673">
        <property type="term" value="F:protein histidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPt" evidence="8">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="197"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="7">
        <location>
            <begin position="456"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="607"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="99"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="2">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="726" mass="76022" checksum="8992B71CFB56720A" modified="2014-01-22" version="1">MSPDPLRYFRIEARELVDQISAGVLDLDQQPGPELVARLLRFAHTLKGAARVVRQKEIADRAHAFEEVLVPHRQGAEALPADEMRELLRLNDEIEAKVTALESPERTSEGDGPETVGTGAKAPQAAAPEEQKAVGQAGTTGPEPGTVEAAHAAAEQARTAPASHNEPTPAARAATADVDELLDAVGEAHARFAPLRTSGHALDRLIRSAESLADQLRVGRTTGQTEGARVAAARLASDLGALGRRVTDTVEQVERELDEVRGRAERLRLVSASSIFTSLHRAVRDAADAQGKRVRFEGRGGELRMDPQVLTLASSALLHVVRNAVAHGIESAGERAAAGKPAEGKVLVEVERRGRYVGFRCTDDGRGFDLAAVRRTAEARGLLMAGGTPPDEQTLVELVLRGGISTSPTVTEVAGRGIGLDVARDVADQLGGDVSVRTEAGRGAVIELVLPLALLSLHGLIVEAADTVATVPLDAVRTCVRLLPEQATAAAVTGKMAHDGEAVPFLPLARALYAGTTVPEGGGPTVAVIVGLDGEQVAVGVDRLAGTSTLVVRPLPDLAPAAPVIGGVSMDLDGNPRLVLDAQGLVAETLRLGGGGAPPAAAAPRLPILVVDDSLTTRMLERSILESAGYEVDLAASGEEGLEKARSRRYGLYLTDIDMPGIDGFTFVSETRADPELATVPAILVSSRASAEDRQRGVAAGASAYVVKGEFNQEELLAHIRRLVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6D5</accession>
    <name>U5W6D5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561036.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32230"/>
    <dbReference type="EnsemblBacteria" id="AGZ44704">
        <property type="protein sequence ID" value="AGZ44704"/>
        <property type="gene ID" value="AFR_32230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6524"/>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_5_11"/>
    <dbReference type="OrthoDB" id="1583074at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="239" mass="24581" checksum="0D56B4EC528C4D16" modified="2014-01-22" version="1">MIVAAAQFAPVAGDVEGNVRTVSGLIRRAGAADARVVVFPELALTGYEPSVIDATPAVCLTEGDARLDPVREACRAAGSAAVVNGPVLTAGAKGGPAITSLVIGPDGALLTRYDKRHLYGFEAEVFTPGTADGRFTLDGVRFSLATCYDNRFPDIAERAASDGCAVYLASSALDTGNDSFDKVYPVRAKDFGLYVVLANLIGPSEAGPCVGRSGVWGPDGERIADAGPDQEGFALAEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6U0</accession>
    <name>U5W6U0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HTH-type transcriptional regulator pecS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ44722">
        <property type="protein sequence ID" value="AGZ44722"/>
        <property type="gene ID" value="AFR_32320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6542"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="15"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="156" mass="17828" checksum="A9E39C6834BB67DA" modified="2014-01-22" version="1">MARWAEFWEDNTDFAPEVEGALVRMKNILRRSRRSDGVAFPDADFTFEDYSTLHALMIQPYPTEATPAQLAEATNVTRAAMTARLDRLAKAELITREVEPQDRRRVIVRPTPAGRAAWERHIHAGMARDQQLLSALTPDELKQLNTLLRKVILSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6V6</accession>
    <name>U5W6V6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_27270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27270"/>
    <dbReference type="EnsemblBacteria" id="AGZ43715">
        <property type="protein sequence ID" value="AGZ43715"/>
        <property type="gene ID" value="AFR_27270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27270"/>
    <dbReference type="HOGENOM" id="CLU_3408467_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="29" mass="3187" checksum="A25C8AF51C5AC63A" modified="2014-01-22" version="1">MRDLGADEVMLYCWATGPEQVDRLADACG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6X4</accession>
    <name>U5W6X4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32470"/>
    <dbReference type="EnsemblBacteria" id="AGZ44752">
        <property type="protein sequence ID" value="AGZ44752"/>
        <property type="gene ID" value="AFR_32470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6571"/>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156416_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="137" mass="15079" checksum="D17B128CFB23F33F" modified="2014-01-22" version="1">MSEIVTTAVADFRALVPDCVAAGLVDMSTGMLLAVDTLDDHPGEVLDLLAAATFDLFQGRNVVMIEDIFKARRGVTSDRHYFQEIVVNSDHLVHLFLRSAKNQDIVAVLVCRRDTKVGMLFAQARIVMKQLEDGFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Y3</accession>
    <name>U5W6Y3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alginate lyase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32520"/>
    <dbReference type="EnsemblBacteria" id="AGZ44762">
        <property type="protein sequence ID" value="AGZ44762"/>
        <property type="gene ID" value="AFR_32520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6581"/>
    <dbReference type="eggNOG" id="COG5297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_691930_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014895">
        <property type="entry name" value="Alginate_lyase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08787">
        <property type="entry name" value="Alginate_lyase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666148" evidence="1">
        <location>
            <begin position="31"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="26"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="398" mass="40853" checksum="602C4564844D1A40" modified="2014-01-22" version="1">MKRRSRLLAVLTTSVVVGTLGVLGITNALAATSGPITGLGGKCVDVASASSANGTAVQLYDCNGSGAQSWTVGNTDNTVQALGKCLDVAAASTANGAKVQLYDCNGSAAQKWTASAGTLVNTGSGKCLDATGQSSANGTQLQIWSCTGTANQAWSLPGGGSTPPPSSGLNPAVAPGGNFNLGIWQLQLPVGSPGSPTTIPSSQLKGANGYVNPTYFFTDKTDGSMTFWAPEKGVTTPNSKYARSELREMNANGSAADWKLAGSHKLNATLRIPNVTKNVCVGQIHLGSGGPSTKPLVELYYRPNGDIYLGTENSPDGGQTLHKVGNVPLGTKWSYTIAVSGGNKINLTVNGSTTSYSIPSSFNPYGMYFKAGSYNQSSSDSTTNGAKVKFYVLNVTHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W728</accession>
    <name>U5W728_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Trimethylamine-N-oxide reductase (Cytochrome c)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_32745</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561145.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32745"/>
    <dbReference type="EnsemblBacteria" id="AGZ44807">
        <property type="protein sequence ID" value="AGZ44807"/>
        <property type="gene ID" value="AFR_32745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6627"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_3_11"/>
    <dbReference type="OrthoDB" id="88184at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02793">
        <property type="entry name" value="MopB_CT_DMSOR-BSOR-TMAOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041954">
        <property type="entry name" value="CT_DMSOR/BSOR/TMAOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041460">
        <property type="entry name" value="Molybdopterin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18364">
        <property type="entry name" value="Molybdopterin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin_N" evidence="6">
        <location>
            <begin position="5"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Molybdopterin" evidence="4">
        <location>
            <begin position="48"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Molydop_binding" evidence="5">
        <location>
            <begin position="593"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="735" mass="79098" checksum="14FE849648FFED67" modified="2014-01-22" version="1">MTTTSSSHWGAFRVSGTGTALQVTPRPGDPDPSPLLGNLAGAHRHRSRVARPALRRPRAGRGYDSFEEVSWDTALDHVAAELSRVREQHGDSAIFGGSYGWASAGRFHHAQSQLHRFLALGGGYTASRNSYSLGASLVALPHLVGRRGAHAVIREGTDWPVIAGHTDLIVAFGGIRTANTYVTPGGRAVHDFRGHLRAATARGLRVASVSPLRDDLSPELAGDWLAIRPGTDVAVMFALAHQLAADGHADLAFLDRFTVGGDRFLADVKDRTPAWAAGVSGLSEQSIVDLARRMAAGRTLVTVSWSLQRTPYGEQPLWAGLALAAVLGQIGLPGGGFGHGYGSMGDVGGGALPYGLPTFPQVRNPVADFIPCARVADLLLHPGTEFDYDGERYTYPDTRLVYWAGGNPFHHHQDLSRLRRAFARPETVIVHEPYWTATARHADVVLPVTTTLERDDLGAGRGDTHLIAMQRADEPYAQARDEYDIFAGLAARLGYHDAFTEGRTSGEWVRHLYEGWRVDRGLPGFDDFWSAGEVVLPGRRENTVLFEDFRAGRPLDTPSGRIELSSATVAGFGYEECPGHPVFHEPALSAYPLVLICNQPGTRLHSQQDMGGVSRASKVHDREPLRLHPADAAARGITDGDVARVFNNLGSLLAGVRLTDACAPGVAQLSTGAWFDPSAPDVATCVHGNPNVLTEDLGTSRLAQGCTGQHARVEITRHDGPLPAIRAFDPPLENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7I6</accession>
    <name>U5W7I6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561411.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34080"/>
    <dbReference type="EnsemblBacteria" id="AGZ45074">
        <property type="protein sequence ID" value="AGZ45074"/>
        <property type="gene ID" value="AFR_34080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6896"/>
    <dbReference type="eggNOG" id="COG3025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074323_0_0_11"/>
    <dbReference type="OrthoDB" id="1475843at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="SSF55154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="233" mass="26350" checksum="69AAD992DF993F6C" modified="2014-01-22" version="1">MGFWQPDPVRVLAGQASEVELKLVVPEHRGRSLGLDPDRKPDRRVYFLDTPDLDLYRNGVIIRFRDRRNQRDDAVIKLRPVEPRRVPGWLRTADRFQVEIDALPDHAVCSGTLKRRLGRNDISRTLAAGRPLTGLLSARQHKLLTRYAPTTDGLIVYGPIDVRCHSIKLRGLSRDLTAERWRYPDGSDLLELSTRCPIGKATTVAARMSAALRAYGVTPADQQRTKTELALLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7J2</accession>
    <name>U5W7J2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PHB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28300"/>
    <dbReference type="EnsemblBacteria" id="AGZ43921">
        <property type="protein sequence ID" value="AGZ43921"/>
        <property type="gene ID" value="AFR_28300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5737"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_905637_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB" evidence="1">
        <location>
            <begin position="20"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="295" mass="31838" checksum="4994C4C30A1FCE30" modified="2014-01-22" version="1">MVGALVLIGAGVGGCTTVSTDSDQVALHYDAGALSSTTYQECVKSNNRSWDGLGEQYYKYPAGQRNFEFSGNEDAESPAFTVVSKDNQELNITGGLTFHLDTSCDDNGGALRAFHEEIGLKFQPVLDDDGKTTDTWLEMLRFYIGQPLAKALTTEAQKYDWLALYNDPVTRQKFETAINASLGASVQATTGDKAYFEKFSLTLQNPEPRKELVDGLAAVQVAITQRQALVEQNATLDEKLKQIRKLVGVLGPDGYILYDTLQRCLTGEAPKGCPSFLPLPPGGTVEAPAPAPAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7U7</accession>
    <name>U5W7U7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 5">Thymidylate synthase</fullName>
            <shortName evidence="5">TS</shortName>
            <shortName evidence="5">TSase</shortName>
            <ecNumber evidence="1 5">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">thyA</name>
        <name evidence="8" type="ORF">AFR_35135</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="1 5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561619.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35135"/>
    <dbReference type="EnsemblBacteria" id="AGZ45283">
        <property type="protein sequence ID" value="AGZ45283"/>
        <property type="gene ID" value="AFR_35135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7109"/>
    <dbReference type="eggNOG" id="COG0207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021669_0_0_11"/>
    <dbReference type="OrthoDB" id="1212177at2"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4 5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylat_synt" evidence="7">
        <location>
            <begin position="5"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="128"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="168"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="209"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="30153" checksum="F14F6B14CA565F25" modified="2014-01-22" version="1">MVDTQYEDLLRRVLETGIPKADRTGTGTVSLFGERLRYDLSQGFPLITTKRVHFKSLAVELLWFLRGDSNTQWLRERGVTIWDEWADKNGDLGPVYGKQWRSWPRPDGGHVDQITELLTTLRQNPDSRRMIVSAWNVSQLPEMALMPCHAMFQFYVADGKLSCQLYQRSADMFLGVPFNIASYALLTQLIAAQVGLVPGDFIWVGGDCHIYSNHVEQVKEQLSRPAYAFPQLEIAPADSLFDYAYEDFKVVGYEHHPALRAPVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W861</accession>
    <name>U5W861_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_34705</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561534.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34705"/>
    <dbReference type="EnsemblBacteria" id="AGZ45197">
        <property type="protein sequence ID" value="AGZ45197"/>
        <property type="gene ID" value="AFR_34705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7022"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_0_11"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="67"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="32176" checksum="1E9DF11CEF940147" modified="2014-01-22" version="1">MKHGRWRFLVGALVPALVLHVVFVLSPYAQAFYLSLTDWTGVAGEAHFVGAGNYARLGGDSLFLDAVRNNAVMLVVVPLVTIGIGLFLASMMHLGGRGGRRGIAGSGAYQIVYFFPQLLSLVVIAVLWGFVYNPNTGLLNGALRGLGLDGLTRSWLASPSLALIAVMAVLIWSSVGFYVVLFSAAIDSIPRELFEAALLDGAGRWATFRRVTLPLVRDSVQVAFVYLGIAALDAFAVVQVMTVGPGGPDGATEVIGLSLYRNAFQYGKFGYASAMGVALFFATLTLAVLALRSGRKERLELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W893</accession>
    <name>U5W893_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29400"/>
    <dbReference type="EnsemblBacteria" id="AGZ44141">
        <property type="protein sequence ID" value="AGZ44141"/>
        <property type="gene ID" value="AFR_29400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5959"/>
    <dbReference type="eggNOG" id="ENOG5033K4F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369725_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666177" evidence="2">
        <location>
            <begin position="26"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="19265" checksum="70466A4ABC7D8136" modified="2014-01-22" version="1">MMVKSVRLGCVAVAAVFGIGACSGGDPEPTPPPVASIVQPSAPASAPAVAERPRLRIDGTEADYQLLLKPYQKCMKAQGVTSEKGTSSWATGDDAPKEQREAQKACEQFWPLPAWERDPANPEAKDFARDVVKCLKGKGVKYVDVEPDGIGITSGGSNNDSRSISLTGEFLDDCEREVAAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8I8</accession>
    <name>U5W8I8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29900"/>
    <dbReference type="EnsemblBacteria" id="AGZ44241">
        <property type="protein sequence ID" value="AGZ44241"/>
        <property type="gene ID" value="AFR_29900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6056"/>
    <dbReference type="eggNOG" id="COG4223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013382_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="514"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="624"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="665"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="692"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="716"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="741"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="801"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="882"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="918" mass="93949" checksum="D36C5855993DFD2A" modified="2014-01-22" version="1">MTNTNPGPDPAETDPHGTDAAAADQQAQDARATDPAEVEAGAATDSADTRLTDAEPVGAETAEVKPVEAKVAGAPDFKEVGSPAEAASADVKRGPDFKEVGSPAGATSAGGKRVPDFKEVGPRAEAAPADVKRGPNIKEVGPSTGTTPADAKRAPAARPTTRRQSWTTQVWRNLRRGLRPDSAPPSRSRRGKTWLPYLLIVLAAGASIAGLAVRPATGAPQETADYVILAGAAGLRWDDLDPQRTPALWQEATRGSIGWLSVRSAHHTTCPADGWLTLGAGNYAIWKDERVEGQCPPVAPALEQPDGIGANLPQLSGVVRENQDKQPYGAVPGALSESLRCTVAVGPGAAVAAARPFGRVDRYEDTLPDDPRDLLSQCVLSMVDLGTVTGSGPARQSLVAQADATLARIVAARPERSVVMVAGVSDTDTTSRLHVAIAEGPGWQGGWLTSAGTGRDGYLQLVDLAPTVLTTLSRPAPDNLFSGHSAFSALGRPANPADAMLGDHNQDKRAGAQLGIAGTFFTGLAAAQLAVFLLVVPLMIRARRHAGPTGPAAAARWLVRGVEVLLIAAALAIPAALLADAAPWWRASRPGIVFALLTFGLVAVGTAAVRFAPRYPRTLWPMGLISAVCATVVGVDLLTGAQLQLNGVAGYSAIEGARYSGVGSVGLGVFVAGILTVAGCLAQWVRRPWRPAVVVLIGGFGVVMVGSPYLGADAVGAIAVTAGVCVAAAISTGGWLTFPRFAWAAIAGLAVTIGFAVLDLRRPEQEQGSLGRALTALGDGTAGPAIQRAATSNGHALDSPLTMLALIGVLMLAFCRFSPWGGLNRVFGLHPALRAAMAGATVATVIAGVLGGAAFAVAGAAAAVAVPLAVLTTLRVLEHAADRTRPEGETDGPGGPGLSMRPILAEVNDKTGVTVRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8J8</accession>
    <name>U5W8J8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="6">OTCase</shortName>
            <ecNumber evidence="3 6">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_30225</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="3 6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560642.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30225"/>
    <dbReference type="EnsemblBacteria" id="AGZ44305">
        <property type="protein sequence ID" value="AGZ44305"/>
        <property type="gene ID" value="AFR_30225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6120"/>
    <dbReference type="eggNOG" id="COG0078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_11"/>
    <dbReference type="OrthoDB" id="988597at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="9">
        <location>
            <begin position="3"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="8">
        <location>
            <begin position="148"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="6">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ornithine binding" evidence="6">
        <location>
            <begin position="228"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="6">
        <location>
            <begin position="264"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ44305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="33059" checksum="C6E0A0A633634971" modified="2014-01-22" version="1">MTRHFLRDDDLSPAEQSAVLELAAAMKADRFAYQPLAGPKSVAVFFDKVSLRTRLSFETGIPELGGNAIVVDTQATHFGRGESLLDAGRVVSRYVSAIVFRTSGDERLAELASDVNVPVVNALTDGFHPCQLLADLQTVRERLGGTQGRILTYVGDGANNMAHSYLLAGATAGMHVRIAGPEGFDPDPEVVARAAEIGASTGGSVQVLRDPREAATGADVLATDAWTSMGMEDDGIDRRTPFWPYQINKELLAAAAPGAIVLHCLPAHRGEEITDEVMDGAQSAVFDQAENRLHAQKALLAWLLAVKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8L0</accession>
    <name>U5W8L0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560657.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30300"/>
    <dbReference type="EnsemblBacteria" id="AGZ44320">
        <property type="protein sequence ID" value="AGZ44320"/>
        <property type="gene ID" value="AFR_30300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6135"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051674_1_1_11"/>
    <dbReference type="OrthoDB" id="1828250at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="26675" checksum="7BB49F8332AEDD9C" modified="2014-01-22" version="1">MNHDVTLRRVVRSEWTKVRSIRSTWIMLAVAALLTLGLAAAFGYGYGQQVRTGELAGTPAAAVSTTFIGLDLFALVTGVFGVLQLTGEYGSGLIRRSLTAVPRCVPLIAAKAVVLLALLVPLSVTVSLTSFLLCQALAGSSGAGLGDDGAVRAVLGASAYPVATALMGLGLGALLRHTAGAVTGFVAGFLIIPALLPAALPQQIQDETLQYLPIAAAQAVYALTPEGPVRLLGPAAGALVLVAWVVAVLAAGTVLLRRRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8L8</accession>
    <name>U5W8L8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30350"/>
    <dbReference type="EnsemblBacteria" id="AGZ44330">
        <property type="protein sequence ID" value="AGZ44330"/>
        <property type="gene ID" value="AFR_30350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6145"/>
    <dbReference type="eggNOG" id="COG2949">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051474_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06259">
        <property type="entry name" value="YdcF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003848">
        <property type="entry name" value="DUF218"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02698">
        <property type="entry name" value="DUF218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF218" evidence="1">
        <location>
            <begin position="62"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="24853" checksum="87151EDBEE76DCDE" modified="2014-01-22" version="1">MFRLTVTGIVTGMLLVWFRRSVVAGSLVVAAMVLAMATGDLWVRAEAHGHVHGVADVPAAPVALVLGAEVYPDGNPSPFLAARLEIARQLLAAGKVKAILVSGDHIAWGYNEPGAMFQWLVDRGVPSQRIVLDHAGFDTYDSCARAKEVFGVTSAIVVTQSYHIDRAVALCRRLGVQASGVGDDTVRIYTEPWRNSVVRERGAVVKALADVVSGRDPVFLGPHEPGVGAALASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8M5</accession>
    <name>U5W8M5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Monovalent cation/H+ antiporter subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_30130</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560623.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30130"/>
    <dbReference type="EnsemblBacteria" id="AGZ44286">
        <property type="protein sequence ID" value="AGZ44286"/>
        <property type="gene ID" value="AFR_30130"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30130"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6103"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_2_0_11"/>
    <dbReference type="OrthoDB" id="1274678at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MbhD-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="DUF4040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="483"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="549"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="581"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="605"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="630"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="670"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="725"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="788"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="812"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="844"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="887"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_N" evidence="10">
        <location>
            <begin position="63"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="9">
        <location>
            <begin position="125"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4040" evidence="12">
        <location>
            <begin position="590"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="MnhB" evidence="11">
        <location>
            <begin position="785"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ44286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="927" mass="96386" checksum="75F60437DEA342DD" modified="2014-01-22" version="1">MLVLLMTHALAAVLAPVLVRRAGRPALYALALVPAAACLWALAQTSRVLDGRPPTERYPWVPQLGAEVALRLTTLPWLMVVLVGGIGAVVLAYSARYFARDDRGLGRFAAVFLAFAGAMAGLVLADDLVVLYVFWELTTVFSYLLIGNDPEHGPSRRAAMQALLVTTVGGLAMLVGFVMLGQHAGTYRLSEIMAAPPPSAGYLGTALALILLGALSKSAIFPFGFWLPAAMTAPTPVSAYLHAASMVKAGIFLIAVLGPAFATDLPWRPMLVIGGLITLISGAWAALRQHDLKLLLAYGTVSQLGLLAVVFGAGNRNAALAGTAMLLAHALFKAALFLVVGIIDHTAGTRDLRELSGLGTRMPVLAVTAALAAASMAGVPPLAGFVGKEAIFAAFTGEPLVLTVLVAGSALTVAYSLRFLWGAFANKPGIEAVKAAPPGWAFVTPAALLAGAGLAAGLAAPVVDRLITGYADEYAAPAYPYHLALWHGPGLPLLLTAVALAAGTVIFLLLRNRAVTPARRGSAYTRVMAGVDRLAVEVTGATQRGSLPFYVGVILVVMVILGGATLLAGTPWPVGVRLWDSPAQAVAGIAVIVAALFAARSRRRLTAMVLVGVSGYGIAVLFVLHGAPDLALTQFLVETVTIVMFVLVLRRLPEEFSARPLAFTRRVRGALALAVGTVTAAMAYAAVGGRQAVPISTEFPDLAVSYGGGTNIVNVALVDIRAWDTMGEISVLVVAATGVASLIFRRTGALERRSAEQGTGRHPAGESAGWLAAGDTPEARRQSIILQVVTRLMFHTVLLFSVYLLFSGHNAIGGGFAGGLVAGLALTVRYLAGGRRELNAAAPVDAGLVLGSGLLVAVGSGVVAMFFGGEVLQSALLDVHLPLLGDLHLATSVFFDVGVYLIVVALVLEILRSLGSELDRQHEREPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8T6</accession>
    <name>U5W8T6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName evidence="10">PRA-PH</shortName>
            <ecNumber evidence="3 10">3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">hisE</name>
        <name evidence="11" type="ORF">AFR_30480</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the PRA-PH family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31" evidence="3 10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560693.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30480"/>
    <dbReference type="EnsemblBacteria" id="AGZ44356">
        <property type="protein sequence ID" value="AGZ44356"/>
        <property type="gene ID" value="AFR_30480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6171"/>
    <dbReference type="eggNOG" id="COG0140">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123337_2_1_11"/>
    <dbReference type="OrthoDB" id="2022633at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ44356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="87" mass="9729" checksum="7F1A9FA69874A902" modified="2014-01-22" version="1">MKTFEELFAELQAKAVERPEGSSTVVALDRGVHFIGKKVVEEAAESWMAAEHEGPERAAEEISQLLYQAQVLMIATGLELEDVYRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8X3</accession>
    <name>U5W8X3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_36055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561803.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36055"/>
    <dbReference type="EnsemblBacteria" id="AGZ45467">
        <property type="protein sequence ID" value="AGZ45467"/>
        <property type="gene ID" value="AFR_36055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7298"/>
    <dbReference type="eggNOG" id="COG0174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_0_3_11"/>
    <dbReference type="OrthoDB" id="416474at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="2">
        <location>
            <begin position="113"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="49232" checksum="522E8B6339106327" modified="2014-01-22" version="1">MDLEDLDVAVDNGTIDTVLLALTDMQGRLQGKRLHGRFFMDEVARGGSEGCNYLLAVDVDMNTVDGYAMSSWNSGYGDFVMKPDFATLRPVPWQPGTAMVLADLETTDGEAVYASPRQILRKQLDRLSAYGLTAFAGTELEFVLYQDTYEQAWTKNYRDLTPANQYNVDYSLLGTARVEPLLRRIRNDMYGAGLIPESAKGECNFGQHEIAFRYADALTCADNHVIYKNGAKEIAAQEGMALSFMAKPNEREGNSCHIHFSLRKEDGTSAMLGDGPAHLSEVGQRVTAGLLATMREFSLLFAPNINSYKRYQPGSFAPTALRWGVDNRTCALRMAGHGQGMRVENRVPGGDVNPYLAIAALVAGAVHGLENELELEDEFRGNAYEDATAPRVPGALRDALELWELSPVAAAAFGDDVVAHYANMAKVEVAAFDAAVTDWELRRGFERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5W983</accession>
    <name>U5W983_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Phosphate acyltransferase</fullName>
            <ecNumber evidence="3 4">2.3.1.274</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Acyl-ACP phosphotransacylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Acyl-[acyl-carrier-protein]--phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Phosphate-acyl-ACP acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">plsX</name>
        <name evidence="6" type="ORF">AFR_36530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>a fatty acyl-[ACP] + phosphate = an acyl phosphate + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.274"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Probably interacts with PlsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Associated with the membrane possibly through PlsY.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PlsX family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.274" evidence="3 4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561898.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36530"/>
    <dbReference type="EnsemblBacteria" id="AGZ45562">
        <property type="protein sequence ID" value="AGZ45562"/>
        <property type="gene ID" value="AFR_36530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7390"/>
    <dbReference type="eggNOG" id="COG0416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039379_1_1_11"/>
    <dbReference type="OrthoDB" id="631784at2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043811">
        <property type="term" value="F:phosphate:acyl-[acyl carrier protein] acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00019">
        <property type="entry name" value="PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003664">
        <property type="entry name" value="FA_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012281">
        <property type="entry name" value="Phospholipid_synth_PlsX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30100">
        <property type="entry name" value="PTHR30100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02504">
        <property type="entry name" value="FA_synthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002465">
        <property type="entry name" value="Phsphlp_syn_PlsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="353" mass="35502" checksum="566BC4ACE1BC75D2" modified="2014-01-22" version="1">MTSPVYGRVAPERRRTSGARAPEQSGRDLSAEAAPEPGIARIAVDLLGGDHAPAVVVDGALRASQADPALQLLLVGPVEAADALLAALDPEARHRVGTITAPAGPGEHDVSRVESGVRTAILAVAQGHADAVVSAGNTGATVTSAALGLGRWPGVRRPPLAAVLPTAAGRLVLLDVGSSVDPDELTLAMHARLGAAYASIVHEVAAPRVGLLTIGTERGKGDRLRRTAPAVLAGLQLPAGARYVGLVEGNDVVTGAKADVVVTDGFTGNVLLKGLETAYALIRPPNDGDVPPRAAILLGVGGTVVVCHGAATGEDVAAGIALAADLHRRAAVPVIADLIQSQVPAHEDPRGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9C9</accession>
    <name>U5W9C9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative MarR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37475"/>
    <dbReference type="EnsemblBacteria" id="AGZ45749">
        <property type="protein sequence ID" value="AGZ45749"/>
        <property type="gene ID" value="AFR_37475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7580"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_4_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="160" mass="17464" checksum="EBBE16A45658AA3E" modified="2014-01-22" version="1">MTDNERLIADIMGAQERLQHLFAHDRSDPLFSSQLTMPQLKILLVLYRLGDTSGRELAGLLGVSLATMSGMVDRLVANNLVTRAEDPHDRRVRRITLSGNGQDMIGKIITAGAEKQARLLGRLTDEELRTVHDGLQAMVRVAAEDAEATSCEASANKAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9K3</accession>
    <name>U5W9K3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ornithine aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ornithine--oxo-acid aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_37875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562164.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37875"/>
    <dbReference type="EnsemblBacteria" id="AGZ45829">
        <property type="protein sequence ID" value="AGZ45829"/>
        <property type="gene ID" value="AFR_37875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7659"/>
    <dbReference type="eggNOG" id="COG4992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_3_11"/>
    <dbReference type="OrthoDB" id="572533at2"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050155">
        <property type="term" value="F:ornithine(lysine) transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine-oxo-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="PTHR11986:SF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="403" mass="42540" checksum="2452CE4E6E8C4190" modified="2014-01-22" version="1">MTDLDLRTPAALAAAERWTAHNYHPLPVVIADAEGAWVTDVDGRRYLDFLAGYSALNFGHRHPGLIAAAHAQLDRLTLTSRAFVHDQFADFCQGLAELCGKDLVLPMNTGAEAVETGIKVARKWGYQVKGVPDGQAEIIVAADNFHGRTTTIISFSTDPDARDDFGPYTPGFKIIPYGDLAALADAITPNTVAVLLEPIQGEAGVLVPVEGYFAGVRELCDDANVLFIADEIQSGLGRTGKTFAIEHEGVVPDMYLLGKALGGGIVPVSAVAANRDVLGVLKPGEHGSTFGGNPLACAVGAEVVRLLATGEFQERSARLGARLHDSLTALIGQGVLAVRGRGLWAGVDIDPALMTGREAVERLAARGVLAKDTHGSTIRLAPPLVVTEEDLDHAVAQLAAILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA04</accession>
    <name>U5WA04_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sulfonate ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_32760</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32760"/>
    <dbReference type="EnsemblBacteria" id="AGZ44810">
        <property type="protein sequence ID" value="AGZ44810"/>
        <property type="gene ID" value="AFR_32760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6630"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="46"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26306" checksum="1DF939FCC1E9F65A" modified="2014-01-22" version="1">MPLLLLVTWELLARAGVIAPDVLPAPSSVGETAWRLTQTGELPTHLWESLKRAAIGLAIGGSIGLVLGTLTGFSRLAEAIVDRNIQIVRAIPFLAILPLVMVWFGVGESGRYFLVALGTLFPIYLNTVLGIRQVDPKLLEMGRVIGLPKLRLVRTVILPGALPSVLLGVRLALTNAWLALVIAETVGAPAGIGFMATNAREFLQTDVIVLVIVLYALIGLSTDLIARALERRLLAWHPNYTGGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAC6</accession>
    <name>U5WAC6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561282.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33435"/>
    <dbReference type="EnsemblBacteria" id="AGZ44945">
        <property type="protein sequence ID" value="AGZ44945"/>
        <property type="gene ID" value="AFR_33435"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33435"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6767"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113325_0_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="197" mass="20953" checksum="AB8A2B703F516221" modified="2014-01-22" version="1">MAPEDRRAALIAATVPLLYEHGLEISTRQIAQAAGVAEGTIFGVFNDKHSLVVAALMEALDPQPTLDAMAAIDAGAGLRERLSAAATLINERFARNARLMTAARSLALSPHSHPDATERMARSRHNLLEGLTEVIAPDAGELRRSPATVARMLLLFCGANTYGPFGDPRSFDGDEIVSLLLDGLLIAPAHRHHHGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAE0</accession>
    <name>U5WAE0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562326.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45992">
        <property type="protein sequence ID" value="AGZ45992"/>
        <property type="gene ID" value="AFR_38690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7828"/>
    <dbReference type="HOGENOM" id="CLU_1575132_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666237" evidence="1">
        <location>
            <begin position="29"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="169" mass="17099" checksum="BE38D978B055D6D8" modified="2014-01-22" version="1">MRKITKRSAAIIAAAVVGVGALGGVAMANGWLVGGKGYATASGAKVSDMRADVTLDKNIYPEVSLTATALVDNPNEFNVELTGIKNPAVTASRGGAANPGCVTALNNLGLAAVTPTLPGTAPIIAKQSTDKTVSLPLTIAKEFPLACQDSIINLTFDFEGTSTVKAANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAN0</accession>
    <name>U5WAN0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38940"/>
    <dbReference type="EnsemblBacteria" id="AGZ46042">
        <property type="protein sequence ID" value="AGZ46042"/>
        <property type="gene ID" value="AFR_38940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7881"/>
    <dbReference type="eggNOG" id="ENOG5033VVH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_628347_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="405" mass="41238" checksum="7DEA3857998972F9" modified="2014-01-22" version="1">MSENATVLLPPRAELRRMVPLFAGRGTYRLVIAVSNVVLATAWGPARFAGYAAAMGAFTVLSFLTALGVEKSALKLIPRARRTGPELITAFVLLPAVLGAGCLVWVAVVATDPLTFAAGALAIGLGANQVLVGLHRVQGRPGLDVANHVALVAGLVVTTLAAALGDLGPTAFVGWTVALVTGLNAALLVELRPRTFRTPRRAVLRAASGTSMLMSAGEVAAAAVVSLVFVVLAHSPHHEQAGALFLAVGVSSLLVKAFGYLLRVVQPQVSVALHPAGAGAAVRARSARNARLLVVAGTPYLIVVTALAVLAGRAGLAPDVVVLLVLYLACAPLIFGMGTINFLLENGGRAMLRRTAAGSVAGLAVAAPAAYLFISHFGAAGGLAAIALGELVHAAAVLHVPERTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAQ3</accession>
    <name>U5WAQ3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF402 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562401.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39065"/>
    <dbReference type="EnsemblBacteria" id="AGZ46067">
        <property type="protein sequence ID" value="AGZ46067"/>
        <property type="gene ID" value="AFR_39065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7906"/>
    <dbReference type="eggNOG" id="COG2306">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083275_1_0_11"/>
    <dbReference type="OrthoDB" id="1473648at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007295">
        <property type="entry name" value="DUF402"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035930">
        <property type="entry name" value="FomD-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04167">
        <property type="entry name" value="DUF402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159234">
        <property type="entry name" value="SSF159234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF402" evidence="1">
        <location>
            <begin position="82"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="21824" checksum="18FE3F40B1E3C39E" modified="2014-01-22" version="1">MRFHHGQQVVRRFLHADRRIAAVRAALVVADDTDGLLLFSDIGAGTMRRTDLAGEPTRHLSIAEELAMPTMLSPGTRGSFRSLLLMPPGAAHSISWSWLEDGTFAGWYVNLETPARRWAGGVDAQDQTLDVLVSAERAWSLKDEADLDPAQAAAVRSEADRVIKLVEAAAFPFDGSLLDYEPPAGWQPAPLPAWWDAVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBA9</accession>
    <name>U5WBA9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_34850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561562.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34850"/>
    <dbReference type="EnsemblBacteria" id="AGZ45226">
        <property type="protein sequence ID" value="AGZ45226"/>
        <property type="gene ID" value="AFR_34850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7051"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_5_11"/>
    <dbReference type="OrthoDB" id="1911275at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="23113" checksum="973EDE705FFF0242" modified="2014-01-22" version="1">MTTVEKPGSAARAVERLRNSMINGELAPGTRLSEASLTGPLEVSRNTLREAFSTLIEEGLLVRQPHRGVFVATLSSAQIADIYRVRVLLECSALQQAPVDPPRAEGMRAAIDEAQRGVAAGDWRAVGTANMHFHEQIVALAGSPRLDAWMRQLTAELRLAFVAAPDVGALHRPFVPMNEEITTIYEGGEAGRAAEVLRTYLLTSERIVLGHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBD2</accession>
    <name>U5WBD2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40215"/>
    <dbReference type="EnsemblBacteria" id="AGZ46297">
        <property type="protein sequence ID" value="AGZ46297"/>
        <property type="gene ID" value="AFR_40215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8139"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042900_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="2">
        <location>
            <begin position="139"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="38534" checksum="8C5E72FE4DAACB04" modified="2014-01-22" version="1">MTQDEFHTALPGHAEFELPVSAPAKRMNGRRPGRRPTQPVVVEGPPMPELTASAPAPEPPPETPRIAGPADVVDVWDQRVASGLAFLRRRLAGAYDVDDFGFDPELTDSVFHPMLKVLYRDWFRTEVFGIENVPDVGGGLVVGNHSGTVALDALMLTVALRDQHPMDRHLRLLGADLVFRMPVLSELARKAGATVACNPDAERLMSEGELVGVFPEGFKGIGKRFSERYKLQRFGRGGFVSAALRTGTPIVPVAIVGAEEIYPILADLKPLARLLGMPYFPVTPTFPWLGPLGLVPLPSKWLIEFCPPIPTAHLTEFADDPMVVYNLADEVRETIQATLHTLLEKRPDAFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBK6</accession>
    <name>U5WBK6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative glutamate ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_35620</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561716.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35620"/>
    <dbReference type="EnsemblBacteria" id="AGZ45380">
        <property type="protein sequence ID" value="AGZ45380"/>
        <property type="gene ID" value="AFR_35620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7208"/>
    <dbReference type="eggNOG" id="COG0765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_1_3_11"/>
    <dbReference type="OrthoDB" id="1523519at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="69"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31709" checksum="58AA1864277C186A" modified="2014-01-22" version="1">MSADAVLYDHPGPRAKIRNTILTVVFGLGLLALVYWIYRKFDEKGQWAAALWKPFTESSTWTDYILPGLWATLSAAAVAMVLSLIFGIVFSVGRLSEHWWLSVPSGIIVEFFRAVPLLLLMFFIFYGVPFITEAPMDPFWAVVIGLTLYNGSVLAEAFRAGIKSVPRGQSEAAYAIGMRKSLVMREILVPQAARAMLPVIVSQLVVLVKDTALGYIIGYSELLQDGVNTLAANFGNIVAAAIVAAIIYIAVNASLTAFAGWLERRTRRQGKAPKNSGPRPAATVETGEAVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBL0</accession>
    <name>U5WBL0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative acyl-CoA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_35425</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35425"/>
    <dbReference type="EnsemblBacteria" id="AGZ45341">
        <property type="protein sequence ID" value="AGZ45341"/>
        <property type="gene ID" value="AFR_35425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7169"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="9"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="628"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="842" mass="87515" checksum="D461782AC64EF076" modified="2014-01-22" version="1">MLLSDGSAVHMRPIRPDDAPAIVEFHSRMSDRTRYLRYFSPYPRIPERDLARFVNVDHRDREAFVIVSGPRIMAVGRYERLGPDAPEAEVAFVVEDAHQGRGIGSVLLEHLAEAAREHGITRFVAEVLPQNAGMLRVFSDFGYQVQRKYADGVVHLEFPIAPTEKSREVQESREQRTEARSISRLLHPRAVAVYGASASGQGIGAALLGHLRDGGYTGTVVPVHRSADRVAGLPAYRSAVAAGVAVDLALVAVPPEGVAEVLADAAAAGAGGLVVVSAGFAEAGAEGARAQRELIDSAHAAGMRVVGPNCLGIANTDPAVRLNATLAPALPAAGRVGFFSQSGALGVALLAEADRRGLGLSSFVSAGNRADVSGNDLLQYWQDDPGTDAVLLYLETFGNPRKFARLARRMSRVKPVVAVASATRPPGLAGDLPGPDTKAVTALFARSGVIRVDTVAELFDVGILLAHQPLPAGRRVAIVGNSSALSGLAVAACRATGLTVADGYPCDISPQAGAHDFADALADAAVDDRVDALVVVFAPPLPGQLPDEDADFAAALGSVALAGEKPTVATFLLGQLPPRVPAYPSVEEAVRALSRVATYADWLRQAPGALPALSGVDPSAPELQGPPTDLLAAYGIKVEPSSSAGSLDEALAAAETLGYPVALKAAGGALRHRIDLGAVRLALADAEDVRAAYSELSQAFGPDVLVQSMVPAGVACTVEVVEDPAFGPVIGFGLGGVASELLGDLAWRAAPLTDRAAAALVDEPRAAPLLHGYRGSAPVDRGALIDLLLRVGRLADEHPQVRALSLNPVLARPDGITVLHASVELGEPGTRPDTGPRRLHTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBN5</accession>
    <name>U5WBN5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_41425</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562871.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41425"/>
    <dbReference type="EnsemblBacteria" id="AGZ46539">
        <property type="protein sequence ID" value="AGZ46539"/>
        <property type="gene ID" value="AFR_41425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8382"/>
    <dbReference type="eggNOG" id="COG1174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_7_1_11"/>
    <dbReference type="OrthoDB" id="1531811at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="27"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="238" mass="24534" checksum="A1E77DF5A22CD47B" modified="2014-01-22" version="1">MNYFHEGYIWLNDPLNWTNPGGLLDRLQEHLLISLWAVLLGCVIGWPLGIWLGHRGRGGGGVITFANLTLAIPTLALLTILPLTPLGFGQPPVVVALAVFAVPPLLANAYTGLRQIDPETRDAARGMGLSGGQVLRQVELPLSVPYLAAGLRTAAVQVVATAALAAFVNGGGLGEIISAGFGIGMSNGGGGQIVAGGIVVALLALLVEAVLAGVQRLVTPPGLRTERRRRPTAAAPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBU5</accession>
    <name>U5WBU5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_42045</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42045"/>
    <dbReference type="EnsemblBacteria" id="AGZ46663">
        <property type="protein sequence ID" value="AGZ46663"/>
        <property type="gene ID" value="AFR_42045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8508"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069543_5_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003012">
        <property type="entry name" value="Tet_transcr_reg_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00400">
        <property type="entry name" value="TETREPRESSOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="6">
        <location>
            <begin position="33"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26643" checksum="24BFB21E2FE10202" modified="2014-01-22" version="1">MTDVIHLAATGGRTGCTSYSWWMPKRSDTTRTRLSRESIVASAIDLADAEGLEAVTIRRLAQQHGVTPMAMYWHFNDKDSLLDGLAEHLIAGVELPEPGDGAAWDVQLRLILERFLEAIRPHPAIAALAMRQILAAESGLQMAERVLGLLRQAGFSAVKAAEVGTFLMCAVITLVASDPSPFPRADDEPGQAVRDKRARLEALSPERYPNVIASAPALVTCREQDGYFGLNLDLLVQGVRGISRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC80</accession>
    <name>U5WC80_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="5">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="5">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ppa</name>
        <name evidence="6" type="ORF">AFR_41565</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + H2O = H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562899.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41565"/>
    <dbReference type="EnsemblBacteria" id="AGZ46567">
        <property type="protein sequence ID" value="AGZ46567"/>
        <property type="gene ID" value="AFR_41565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8410"/>
    <dbReference type="eggNOG" id="COG0221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073198_1_1_11"/>
    <dbReference type="OrthoDB" id="1767807at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="PTHR10286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="SSF50324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="19247" checksum="1D3044ED17EBFC98" modified="2014-01-22" version="1">MDFDVLVEIPKGQRNKYEVDHKTGRIRLDRTLFTATQYPADYGFIEGTLGQDGDPLDALVLIQEPTFPGCLVRARAIGMYRMTDEKGRDDKVLCVPYEDPRQEHLRDIHHLGEFDRMEIQHFFTVYKDLEPGKSVEGATWTGRVEAEEEIRASFKRAEAAEHHDEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCB1</accession>
    <name>U5WCB1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="15">DAD</shortName>
            <ecNumber evidence="14 15">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ilvD</name>
        <name evidence="17" type="ORF">AFR_36905</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="15">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14 15"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36905"/>
    <dbReference type="EnsemblBacteria" id="AGZ45635">
        <property type="protein sequence ID" value="AGZ45635"/>
        <property type="gene ID" value="AFR_36905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7467"/>
    <dbReference type="eggNOG" id="COG0129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_4_2_11"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="568"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="568"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="15">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ45635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="615" mass="65211" checksum="6039B698749A5A6D" modified="2014-01-22" version="1">MPELRSRTSTHGRTMAGARALWRATGMTDDDFGKPIVAIANSFTQFVPGHVHLKDMGGLVADAVFEAGGIGREFNTIAVDDGIAMGHGGMLYSLPSRELIADAVEYMVNAHCADALVCISNCDKITPGMLIAALRLNIPTVFVSGGPMEAGKTIAIEGVVHEKLDLVDAMSASANENVTDEQLDTIERSACPTCGSCSGMFTANSMNCLTEAIGLALPGNGSTLATHAARKALFERAGELIVDIAKQYYQNDDESVLPRSIANRDAFENAVALDVAMGGSTNTVLHLLAAAREAEIDFSVADIDAVSRRVPCLSKVAPNSPKYHMEDVHRAGGIPALLGELHRGGALRPNVRSVHSPDLDSWLQEWDIRSGQASEKALELFHAAPGGVRTTQPFSTTNRWATLDTDAAEGCIRSVEHAYTVDGGLAILFGNLAPDGCVVKTAGVAEELWKFSGPARVFESQDDAVDGILGKRVLAGDVVVIRYEGPRGGPGMQEMLYPTSFLKGRGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGLIALVQTGDEITIDIPGRTITLNVTDEELAQRRHEEERRPKPYSPVDRQRTVSAALRAYASMATSASDGAYRKVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCB8</accession>
    <name>U5WCB8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_41790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562943.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41790"/>
    <dbReference type="EnsemblBacteria" id="AGZ46612">
        <property type="protein sequence ID" value="AGZ46612"/>
        <property type="gene ID" value="AFR_41790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8457"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_9_0_11"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666276" evidence="2">
        <location>
            <begin position="22"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="3">
        <location>
            <begin position="106"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="591" mass="62719" checksum="C747813D07BCD400" modified="2014-01-22" version="1">MVSKTRVVAAAGVAVALGFTAACGGGSDSDSNGGGTSGGKSAYNAAITSVVNPSTAKGGTLNLMTAQDADSWDPARGYYAFVWDMNRFYTRKLVDYAAVPGKEGLKLVPDLATADAEISADKKTYTYKLKPGLKFDDGAAITSKDIKYGIERIFAQDVVPGGPTYLIDQLDQGQNYPGPYKDTDPNKLGLKTVETPDDSTIIFKLKAPFADFPYLLAMPGAGPVPQKRDTGAQYGAKPASSGPYMITDYQPAKSLKLERNPNWDASTDTIRKALPDAINVTITTNADDMDQRLEAGTADLDIGQTGVQAAARARILQDPAKKANSDTPATGFIRYAGIVQKVAPFENIECRKAVIYASDPTSLQAARGGPVAGGDIGTSMLPPNIAGSDPSYDPYGRAQGKPQVDKAKQALAACGKPTGFDTTIAVRNNKPAEVKTAEALQAALKTVGINAKIDQYDGAQVASVTGSPDNVHKKGYGIIIGGWAADWPSGTGYLQALADSRYIQKNGNYNLPEINDPAIDGLFDQAATETDLSKADAIYKQLNTKVMEGAYYLPFVFDKALNYRNPRLTNVYIHDTFGMVDFQSLGVSDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCR6</accession>
    <name>U5WCR6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Prephenate dehydratase</fullName>
            <shortName evidence="10">PDT</shortName>
            <ecNumber evidence="3 10">4.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pheA</name>
        <name evidence="13" type="ORF">AFR_42565</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:21648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="4.2.1.51"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="3 10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563098.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42565"/>
    <dbReference type="EnsemblBacteria" id="AGZ46767">
        <property type="protein sequence ID" value="AGZ46767"/>
        <property type="gene ID" value="AFR_42565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8614"/>
    <dbReference type="eggNOG" id="COG0077">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_0_0_11"/>
    <dbReference type="OrthoDB" id="1280729at2"/>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008242">
        <property type="entry name" value="Chor_mutase/pphenate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001500">
        <property type="entry name" value="Chor_mut_pdt_Ppr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00858">
        <property type="entry name" value="PREPHENATE_DEHYDR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 10" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="11">
        <location>
            <begin position="8"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="12">
        <location>
            <begin position="201"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="site" description="Essential for prephenate dehydratase activity" evidence="9">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001500-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ46767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="33144" checksum="2B1B3359FDFB4A6D" modified="2014-01-22" version="1">MPGTPPTRFVYLGPEGTFTEQALRTISAAEHGIRTPARSVPEALEAVRTGEADAALVPLENSVGGAVPVTLDELGTGSPLVITREVVLPVEFVLAAPALVPLAGIRSVAAHPQASAQCRNWLLANLPDAVVVDVLSNAAAAISAASGEHDAAICAPIGVPRNNLTVLADKIADHADAVTRFALLSRPAAPAPPTGDDITSLAVMIDHDRVGALLSVLTELAVRGINLSRIESRPTGEQLGVYVFFLDCGGHVAEPRLGEALQGLRRICAEVRFLGSYPRHRWSKAAAAEPAPSQPGLTNADFADSAAWLTRLRSGEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCS6</accession>
    <name>U5WCS6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative AsnC-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_42615</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563108.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42615"/>
    <dbReference type="EnsemblBacteria" id="AGZ46777">
        <property type="protein sequence ID" value="AGZ46777"/>
        <property type="gene ID" value="AFR_42615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8624"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_0_11"/>
    <dbReference type="OrthoDB" id="1844486at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="16363" checksum="2944892E6A4D01FC" modified="2014-01-22" version="1">MQMDAVDQRIIALLVADARASYAEIGAKVSLSAPAVKRRVDRLRSSGVIKGFTTVIEPAAVGWTTEAFVELFCTGRTTPAQITVATRRHPEVVGAYTVSGQADALVHLRAADIGHLEQALERLRAEPFVTSTRSMVVLSRLVEMPTPVPAPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDJ5</accession>
    <name>U5WDJ5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP_transf_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562394.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39030"/>
    <dbReference type="EnsemblBacteria" id="AGZ46060">
        <property type="protein sequence ID" value="AGZ46060"/>
        <property type="gene ID" value="AFR_39030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7899"/>
    <dbReference type="eggNOG" id="COG1708">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069366_1_0_11"/>
    <dbReference type="OrthoDB" id="1862840at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_2" evidence="1">
        <location>
            <begin position="19"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="28687" checksum="4E8D7CB71EAA54E2" modified="2014-01-22" version="1">MALSDERLHELAEGLVRVPGVTGVVLGGSRARGTHTPESDTDLGVYYRKPLDVAALGALAVEFGGPAAQVTEPGGWGPWVDGGGWLTIDGGAVDWIYREVGRVEQSCTDAEAGRYTFHSQTGHPLGVPDFAYAGELALGVILADPSGELAALRARAGVYPPALRDSLIAGLWEADFLLGLGRKAVTRADSAYLAGCLFRVAGVCAHALHGAAGRWLINEKGAVAAAAALPGAPPRFKERLDAVFAGLDGDPVHLVAALDAAQDLVLETADACAMMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDV7</accession>
    <name>U5WDV7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exodeoxyribonuclease 7 large subunit</fullName>
            <ecNumber evidence="5">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Exodeoxyribonuclease VII large subunit</fullName>
            <shortName evidence="5">Exonuclease VII large subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">xseA</name>
        <name evidence="9" type="ORF">AFR_39210</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the XseA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562430.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39210"/>
    <dbReference type="EnsemblBacteria" id="AGZ46096">
        <property type="protein sequence ID" value="AGZ46096"/>
        <property type="gene ID" value="AFR_39210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7934"/>
    <dbReference type="eggNOG" id="COG1570">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023625_2_1_11"/>
    <dbReference type="OrthoDB" id="1371775at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04489">
        <property type="entry name" value="ExoVII_LU_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00378">
        <property type="entry name" value="Exonuc_7_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003753">
        <property type="entry name" value="Exonuc_VII_L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020579">
        <property type="entry name" value="Exonuc_VII_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025824">
        <property type="entry name" value="OB-fold_nuc-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30008">
        <property type="entry name" value="PTHR30008"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02601">
        <property type="entry name" value="Exonuc_VII_L"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13742">
        <property type="entry name" value="tRNA_anti_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00237">
        <property type="entry name" value="xseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_anti_2" evidence="8">
        <location>
            <begin position="17"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuc_VII_L" evidence="7">
        <location>
            <begin position="133"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuc_VII_L" evidence="7">
        <location>
            <begin position="334"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="405" mass="45006" checksum="C8B3469F62AF51AC" modified="2014-01-22" version="1">MTQPETPKSSADEPWPVRVVSQKLSAWIAKLGWVWVDGQVAQISRRPGASVVFLTLRDPSADLSLTVTAHRDVLDAGAPELAEGARVTMHAKPEFYPARGSLSLRADEIRQVGLGELLARLERLKKLLGAEGLFARERKRRLPFLPQRVGLITGRASAAERDVLMNTRRRWPSVDFRVINVPVQGPTAVPQIIDAMKVLDNDETVDVIVIARGGGSVEDLLPFSDEALCRAVFGCRTPVVSAIGHETDAPLIDYVADVRASTPTDAAKRIVPDLAEETTLIQQARRRLDRAVITLVDRESHRLESWRSRPVLARPEVLVDQRETEVNALRDRATRSLDQRLRRADDDLRHTLARLRALSPKATLERGYAIVQRPDGHVVRAADEVETGDLLRVRLADGELRAEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE92</accession>
    <name>U5WE92_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flagellar protein FlbD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">flbD</name>
        <name evidence="2" type="ORF">AFR_40480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562682.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40480"/>
    <dbReference type="EnsemblBacteria" id="AGZ46350">
        <property type="protein sequence ID" value="AGZ46350"/>
        <property type="gene ID" value="AFR_40480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8193"/>
    <dbReference type="eggNOG" id="COG1582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173020_0_0_11"/>
    <dbReference type="OrthoDB" id="2007051at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009384">
        <property type="entry name" value="SwrD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39185">
        <property type="entry name" value="PTHR39185"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06289">
        <property type="entry name" value="FlbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="95" mass="10400" checksum="05C3EAF2CE12C93C" modified="2014-01-22" version="1">MILVTRLNGAVFALNPDLVERADCTPDTVITLVDGTKYVIAESVPEFIDSVRHYRASLIAQASRIEAEPALLAEPAPSDDENAATNVLQLHRKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFB2</accession>
    <name>U5WFB2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41710"/>
    <dbReference type="EnsemblBacteria" id="AGZ46596">
        <property type="protein sequence ID" value="AGZ46596"/>
        <property type="gene ID" value="AFR_41710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8441"/>
    <dbReference type="eggNOG" id="COG3287">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052774_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019494">
        <property type="entry name" value="FIST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013702">
        <property type="entry name" value="FIST_domain_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08495">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10442">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00897">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01204">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIST" evidence="1">
        <location>
            <begin position="38"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="FIST_C" evidence="2">
        <location>
            <begin position="233"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="386" mass="39421" checksum="A4915BA123B2D3E2" modified="2014-01-22" version="1">MIIMQSATGRWFGTGHSTAPDSGKAGAEAAGAALAGRTPKAVFVFCSAGHDLDALLAGVRAEAGPDAAVVGATTLGELSSGGGPARGSVAVTALGGDGFTVRTRVAHIGGTGHREAGADVAAAMAGMDRPHSVLMLLCDGFSGDPHEIVRGAYSVVGAGVPLVGGFAGDDRGTRRSFQFHQDRALTGTVVGVAIGSDAPLGVGIAHGWHRVEPPMVVTRSDGGRIYELDGEPALDVLLRKRGLDSINLGPTDLQSLGLSRRSGVDIRVVHGYDEKDRSVVSGATVPQGALCWLMDSERQSLIEGAERSCAEAVEMLGDAEPLGVFTFNCAGRWGALGDDGVQEEVAGMRAVLGDVPFGGFYTMGEIARVRGSLGTHALTLVTLAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFL6</accession>
    <name>U5WFL6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563047.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42310"/>
    <dbReference type="EnsemblBacteria" id="AGZ46716">
        <property type="protein sequence ID" value="AGZ46716"/>
        <property type="gene ID" value="AFR_42310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8563"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_4_11"/>
    <dbReference type="OrthoDB" id="1027479at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05276">
        <property type="entry name" value="p53_inducible_oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014189">
        <property type="entry name" value="Quinone_OxRdtase_PIG3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02824">
        <property type="entry name" value="quinone_pig3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="7"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="321" mass="33561" checksum="C3D24961BC1A74E1" modified="2014-01-22" version="1">MHAITIEQPGSFTWAEVPDPTPAEGEVVVEVTASAVNRADVMQRQGHYPPPPGAPPYPGLECSGVITAVGPGVTGHHVGERVCALLAGGGYAEQVAVPAGQLLPIPQGLSLQEAAALPEVACTVWSNVVDIARLRQGETLLVHGGGGGIGTFAIQLGKALGATVIATARESKHAQLKDLGADLTIDYTSTDFVEATRDFTDGRGANVILDIIGARYLDKNVAALAPDGRIAIIGMQGGRRGEFDLGALMTKRGSISAATLRARPIPDKARIVRGVHERIWPLVGAGTIRPIIDRTMAMSEAAEAHRLMESSDHIGKILLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNB3</accession>
    <name>U5VNB3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">6-phosphogluconate dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_00015</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357236.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00015"/>
    <dbReference type="EnsemblBacteria" id="AGZ38292">
        <property type="protein sequence ID" value="AGZ38292"/>
        <property type="gene ID" value="AFR_00015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3"/>
    <dbReference type="eggNOG" id="COG1023">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024540_0_0_11"/>
    <dbReference type="OrthoDB" id="1245550at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004849">
        <property type="entry name" value="6DGDH_YqeC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="PTHR11811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00872">
        <property type="entry name" value="gnd_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6PGD" evidence="3">
        <location>
            <begin position="160"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="290" mass="31158" checksum="4F0E4BBA6DE224B7" modified="2014-01-22" version="1">MQLGLIGLGRMGGNMRDRLRAAGHEVVGYDHHKETTDVASLAELVEKLAAPRVIWTMVPAGQITESTIDELAELMSDGDIIIDGGNSKFTDDAPRAERLKPKGISYIDCGVSGGIWGITNGYALMVGGDAATVAHCQPIFDALKPAGEFGFAHAGTHGAGHYAKMIHNGIEYGMMQAYAEGYEILTAAEIVENVPAIIKSWREGSVVKSWLLDLLDRALDEDPQLANLKGYVDDTGEGRWTIDEAVRLAVPANVLAASLFVRFGSRQEDSPAMKAVAALRNQFGGHAVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNV7</accession>
    <name>U5VNV7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357561.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01645"/>
    <dbReference type="EnsemblBacteria" id="AGZ38618">
        <property type="protein sequence ID" value="AGZ38618"/>
        <property type="gene ID" value="AFR_01645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.331"/>
    <dbReference type="eggNOG" id="COG4022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191176_0_0_11"/>
    <dbReference type="OrthoDB" id="2018206at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="10583" checksum="B4FA40E8CFCE054E" modified="2014-01-22" version="1">MTTIDYLVDDSDIDVPIDDRDEGERRALELAHRIRQVAVTDPEHAQQLVDELIVALDAAMGGTFSEHLDGVAREVAERALEKSRNGHGAGISPYGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP44</accession>
    <name>U5VP44_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA polymerase sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_01390</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357510.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01390"/>
    <dbReference type="EnsemblBacteria" id="AGZ38567">
        <property type="protein sequence ID" value="AGZ38567"/>
        <property type="gene ID" value="AFR_01390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.279"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_15_7_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="5">
        <location>
            <begin position="12"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="6">
        <location>
            <begin position="100"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="18886" checksum="67016B8235C5E40D" modified="2014-01-22" version="1">MVEHAGTKFEDFYRAELRPLIWFAVRVGAARAETEDVAQEAMKAAWAGWARIENPRAYVRTAVLRVVRRTGYQVKRNQEAALRAAGPPGVGAESFEAEARRVLDLLRALPEAQREVMALTMDGYDPLAISQITGRKDSTVRSHLRHARRNLTRAIDEHHPEKEANDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP59</accession>
    <name>U5VP59_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator nadR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357696.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02320"/>
    <dbReference type="EnsemblBacteria" id="AGZ38753">
        <property type="protein sequence ID" value="AGZ38753"/>
        <property type="gene ID" value="AFR_02320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.469"/>
    <dbReference type="eggNOG" id="COG3172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052648_0_0_11"/>
    <dbReference type="OrthoDB" id="693749at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038727">
        <property type="entry name" value="NadR/Ttd14_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13521">
        <property type="entry name" value="AAA_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_28" evidence="1">
        <location>
            <begin position="157"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="357" mass="39065" checksum="685AA9AFA188C2FA" modified="2014-01-22" version="1">MTWGHGLIVGKFYPPHAGHHALIDAAAAACERVTVVVAPSSHESVPLDLRLAWLRETHTANVRFVGVHDDHPVDYADPAVWDLHVAVFRAAAGPGPVDAVFSSEAYGEELARRFSAEAVTVDLDRIKVPVSGTAVRADPVAHWDLLGPGVRSWLCRRVIVVGAESSGTTTMARALARHYRTRGGVWAQTRWVPEFGRELTVRKLDALRRSRPEATVDEVVWGRDDFMTVADEQSAAEDWAARESSPLVIGDTDALATTIWEERYLGSGSAAVRERVRPPSLYLLTDHEQVPFVDDGLRDGEAVRAWMTGRFREVLAAQGVPWRVLTGSYGDRLRAAVAACDEVLASGWSFAPPLSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP96</accession>
    <name>U5VP96_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02495"/>
    <dbReference type="EnsemblBacteria" id="AGZ38788">
        <property type="protein sequence ID" value="AGZ38788"/>
        <property type="gene ID" value="AFR_02495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02495"/>
    <dbReference type="HOGENOM" id="CLU_1727433_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16546" checksum="1BFC3C5AED13D6DA" modified="2014-01-22" version="1">MSVRVAAPVLVQEPALVLALVRESVLALVPVREPVPVLVRVRVLALGRVRMQMSVLAPVQAPARERVRVPVPVQARAFPVQVRAFPVQVQACPRQPVPARIRRLVVASLLALAPALRLPPEPTWAPRLGLLSVRARIRQLGPARTRPQVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPP3</accession>
    <name>U5VPP3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_03220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357876.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03220"/>
    <dbReference type="EnsemblBacteria" id="AGZ38933">
        <property type="protein sequence ID" value="AGZ38933"/>
        <property type="gene ID" value="AFR_03220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.648"/>
    <dbReference type="eggNOG" id="COG1172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028880_0_2_11"/>
    <dbReference type="OrthoDB" id="1254678at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="35953" checksum="527FB70C055F83DE" modified="2014-01-22" version="1">MTTTATKEPSPQEFDVRRNDSLGLRLQHLLHGNPTLGPLAVLIVAIIAFSIANQRFFSAANLSLVLAQVTVIAVLALGQTLIILTAGIDLSVGAVAVFSSILMASFATKIGVPGLLALILGLICGTAMGALNGFLVTRIKLPPFIVTLGTLSIFFSLNAVVSNSETIRGSDMPGIMTWTGTTIPIGGFRLTYGSIIMLLLFAFFFYALGSTAWGKHVYATGDDIESARLAGIRTNRVLFSVYTVAGLLYAVGAWILIGRLASASPNVGVDYNLDSITAVVLGGTSLFGGRGGVLGTLIGALIVGVFRNGLQLAGVEVVWQGFAIGLLVLVAVSLDQWIRKVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPR4</accession>
    <name>U5VPR4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase central domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbxX/CfxQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02565"/>
    <dbReference type="EnsemblBacteria" id="AGZ38802">
        <property type="protein sequence ID" value="AGZ38802"/>
        <property type="gene ID" value="AFR_02565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.516"/>
    <dbReference type="eggNOG" id="COG0464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008749_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041627">
        <property type="entry name" value="AAA_lid_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000641">
        <property type="entry name" value="CbxX/CfxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17866">
        <property type="entry name" value="AAA_lid_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00819">
        <property type="entry name" value="CBXCFQXSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="586"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="876"/>
            <end position="1074"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1097" mass="114124" checksum="6C333A796B9A39D0" modified="2014-01-22" version="1">MLTVGGQRAGSFPTIGAALARAQAGATISVHPGRYAEKLVVGDRITISAEPGGAVEVFVEEGSVLAVHGEGAQLRGITLSSADPKLAAVDVYSGEVALDDCRISGSAWVTLLARLQGSLALRGCEVRNSGGAGIVVTAPGTSTVEDTVVRDVSTSGIVVGEQGSLILRRCVVRDTGANSICVNGDAKLVMEQCEIIGAGKPALVVEQRGRARVNRLTVRGSANVDVFLRSEVDVVITDSEFTGAALQSVHIADGAAPEFKGCTFASAGHTAAHITGKARPVFTDCTVTDSKIGVNVDGAAAPRFTGLTVRGTSDHVAIVSAEASATVDRLRSTVTQGAGLLVKDGASLDGSDLQVESGNAVALELVGAARGTVREARFTGTGTASLTIDGGSTLDLRSTVLRGAGLRLGDASELQIRDSEIVDASGDGLLVTAGATVTATQTRVRAARLAGVRWEAGSRGALTDCEILGSGGAGISVETTEPVSITRCVVQDSGAEDLHRGADAQPVVESLTTGGRATPAAAAYVPEPEQADESAPPEPEGELSGPLRELNSLIGLRGVKHEVTALINLIKMSQVRLQMGLPMPPMSRHLVFAGPPGTGKTTVARLYGSVLAELGILSKGHMVEAARADLVGQYIGSTAIKTTELVTKALGGVLFIDEAYTLSAGSGGSGPDFGQEAIDALMKMMEDHRDELVVIVAGYSELMERFLESNPGLASRFTRTVEFPNYSVAELVTITSNLCSKHYYDLTDDAIDALTTYFERVPKNSTFGNGRVARKLFEAMINNQASRLATVPPTKDIELNRLTGADLAPELALLEDLPVEHVTQPDSATNPTGAINAARSWRRVCDLVGATGVRDAVGETLLQLCELRNRRRAYGRHGNVLLGGRSGSGRSEFARLYAAGLSELDLVPVGHLVRVSTGQELAPQWPGQARSLVRAAMQDAEGGVLVVDYTDDGSGNGVEIVESLVEQMRAGPGDPVVVLIGEDAALAHLRTTVPGLAEAFGQEWSVPDYTTAELADIVVRHLVRRGHEVPDDVRQAITGLTTGLSERTVRAAHLFSWGLTRTAASRTLALADLTGLAGRGSPDPVPARQAGGLAAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ86</accession>
    <name>U5VQ86_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_03425</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357917.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03425"/>
    <dbReference type="EnsemblBacteria" id="AGZ38974">
        <property type="protein sequence ID" value="AGZ38974"/>
        <property type="gene ID" value="AFR_03425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.689"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="32079" checksum="5097F728386ADF4B" modified="2014-01-22" version="1">MIEVHELTKRYGAKTAVDSLTFTVKPGIVTGFLGPNGAGKSTTMRMILGLDRPTSGTATINGRPYAEHTAPLKQAGALLDAKAVHTGRSAYHHLLAMAATTGVPRSRVDEVIDLVGLHEVARKRAGAFSLGMGQRLGIASALLADPEVLILDEPVNGLDPEGILWIRHLMQNFAAQGRTVFVSSHLMSEMALTAEHLVVVGKGRLIADVSVAAFIEQAARDTVLVRSPRAVELHDLITGPGVTVLSVERGAFEVSGLSAAEVGDRASAAGIALHELTVQRPSLEEAFMELTKDAVEYQTVGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQE6</accession>
    <name>U5VQE6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358101.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04320"/>
    <dbReference type="EnsemblBacteria" id="AGZ39153">
        <property type="protein sequence ID" value="AGZ39153"/>
        <property type="gene ID" value="AFR_04320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.872"/>
    <dbReference type="eggNOG" id="COG3393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064724_2_0_11"/>
    <dbReference type="OrthoDB" id="1270792at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013653">
        <property type="entry name" value="FR47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08445">
        <property type="entry name" value="FR47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="138"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="29539" checksum="060B2FBB0EA4A60A" modified="2014-01-22" version="1">MAWHITDNVDDFLAGAGEFLRSRPVENTVLLTVTDNVRQRGSAAYGDEPPMFGWCDDGAFLRTPPHSALLSVMSPADATDLAALLADTQLPSVIGPDATVEAFAAEWQRRTGTTAAEKGRQRLFRLGTLVPPTPAAPGAARVAGAEDRDLLVAWMTTFQREIGELPQHTGDFVDDKLSYGGLSLWEVNGEPVSMAGLTRNKAGMVRVQAVYTPREHRTHGYAGAITTAVSRAALDAGATDVVLFTDLANPTSNALYQRIGFTPIEDRTLVEFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR25</accession>
    <name>U5VR25_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="6">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tsaD</name>
        <name evidence="8" type="ORF">AFR_04865</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358304.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04865"/>
    <dbReference type="EnsemblBacteria" id="AGZ39262">
        <property type="protein sequence ID" value="AGZ39262"/>
        <property type="gene ID" value="AFR_04865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.982"/>
    <dbReference type="eggNOG" id="COG0533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_0_2_11"/>
    <dbReference type="OrthoDB" id="1257362at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="PTHR11735:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03723">
        <property type="entry name" value="T6A_TsaD_YgjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TsaD" evidence="7">
        <location>
            <begin position="27"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="137"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="350" mass="35669" checksum="5CBD5BA717D61E47" modified="2014-01-22" version="1">MPDEPLVLGIETSCDETGVGIVRGNTLLADALASSVEQHARFGGVVPEVASRAHLEAIVPTMQRALDEAKVTLADIDAIAVTAGPGLAGALLVGVAAAKGYALAADKPIYGVNHLAAHVAVDTLEHGPLPEPAIAMLVSGGHSSLLLVDDLTAGVTPLGATIDDAAGEAFDKVARLLGLGFPGGPVIDRLAREGDRGSIAFPRGLTAPKDQLKHRFDFSFSGLKTAVARWVEARERAGEPVPVADVAASFQDAVCDVLTAKALDACRENGVETLIIGGGVAANSRLRVLAEERAAKLGIRVRVPRPGLCTDNGAMVAALGSHLVAAGVAPSRLDLPADSAMPLTSVTVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRW1</accession>
    <name>U5VRW1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00585"/>
    <dbReference type="EnsemblBacteria" id="AGZ38406">
        <property type="protein sequence ID" value="AGZ38406"/>
        <property type="gene ID" value="AFR_00585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00585"/>
    <dbReference type="HOGENOM" id="CLU_2949852_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="67" mass="6835" checksum="2F5805497F1B8D0F" modified="2014-01-22" version="1">MGRLTPGAGIESSTAMSSDQKAAEALQEAETEVGVAEMVDRGADAAVRGAGQTYRSAYRSTDLGLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS46</accession>
    <name>U5VS46_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_01230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357478.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01230"/>
    <dbReference type="EnsemblBacteria" id="AGZ38535">
        <property type="protein sequence ID" value="AGZ38535"/>
        <property type="gene ID" value="AFR_01230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.245"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_76_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="231"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="148"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="48719" checksum="4F9EAD18348B66DE" modified="2014-01-22" version="1">MTSPDPLIRARIRVEQDIFVVRQLAREVAREVGMETQDQTRLATALSEVGRLMLVTGRDAEVVFVAAPDGVPTLQVQVEYPVTGDAARLTSQLGQVRRLVDSMEVGDGGIGTIVRMSRRLPPAAPLLSPDLMDEIRERLAGHVPGTPYDELTVQNQQLIGALDEVRAQRDDLARLNAELEETNRGVMALYHQLSDELEETNRGVVALYNELDEKSVQLRAASEAKSRFLANVSHELRAPVTAIIGLGRLLSDDASDPLTPEQNRQVELIRGSATDLLSLVNDLLDLAKAEAGRIEPNWSDVDLKAVFGQLRGTLRPLATRPEVDFVVEEPSIAVLRSDEVLLAQVLRNLLTNAIKFTSEGSIRLAVTPIDDGVEFTVADTGTGIPPELQERIFEEFYQVPGSHPLSGKGTGLGLPYARRLVTILGGALRVTSTPGQGSTFTVSLPVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS94</accession>
    <name>U5VS94_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39677">
        <property type="protein sequence ID" value="AGZ39677"/>
        <property type="gene ID" value="AFR_06940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1416"/>
    <dbReference type="HOGENOM" id="CLU_1615476_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="164" mass="16756" checksum="2197D62960B01305" modified="2014-01-22" version="1">MDRGPMALFGAIIALGLGPAMWVGAQFGQVTLTPDRPPAVISNQQNQAPEGGSGAGDAPAGTEVIRTEPKSNNEPLKPTKNSRPVKTSPSPSASDEPSAPVDESTPPDSTDPADEETTPPTESTTPPVDPPTEDEDPDVPPEPPVQPGDDNTDTATQAGESSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSB3</accession>
    <name>U5VSB3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Peptidase M48 Ste24p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_07300</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359280.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07300"/>
    <dbReference type="EnsemblBacteria" id="AGZ39749">
        <property type="protein sequence ID" value="AGZ39749"/>
        <property type="gene ID" value="AFR_07300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1490"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052979_0_0_11"/>
    <dbReference type="OrthoDB" id="1918093at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="9">
        <location>
            <begin position="75"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="356" mass="38971" checksum="21F589636986F34B" modified="2014-01-22" version="1">MTTEDDNRPARRRITLTGISSRAWEHPADRGALTALRELRGFDDVVKAFFGMWNERGFRLSFLAGSIRVDHRQYPRVYQRFTEAAATLDVAELPELYVSQSPMINGQAIGMDKPFIVITTAAVEKLDDEELRALLGHEIGHVRSGHAVYKTIMMILTKWAASLSWLPVGAIALRAIIAAMYEWWRKAELSADRAGLLAGQDPAASLRLLMKLAGGGDLSQIDTTAFLEQAAEYEGGGDLRDSIHKLGMTAWSTHPVPVARASELRKWIDSGDYGRILEGEYPRRDSDGNASVTDDVKAAADSYRQSFNSSQDPLVGLLRKLGDGAADLAGSAARGARGWASKGRRPEEEPPPPPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSN7</accession>
    <name>U5VSN7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Multidrug-efflux transporter 1 regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359534.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08570"/>
    <dbReference type="EnsemblBacteria" id="AGZ40003">
        <property type="protein sequence ID" value="AGZ40003"/>
        <property type="gene ID" value="AFR_08570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1744"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4978">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065103_2_2_11"/>
    <dbReference type="OrthoDB" id="1748122at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010499">
        <property type="entry name" value="AraC_E-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029442">
        <property type="entry name" value="GyrI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06445">
        <property type="entry name" value="GyrI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00871">
        <property type="entry name" value="AraC_E_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="280" mass="30549" checksum="774F8CA7977F0C9B" modified="2014-01-22" version="1">MFSIGDFAALGRVSVRMLRHYDSIGLIRPARVDQFSGYRFYTAAQLTLLNRVLALKDLGFTLQQVQTLIDEKVDVGELRGMLRLRRAQLAEEMAAGTARLALVDARLRMIETEGHMNTGDVILKTVPPVRVAELSAPASSYDGPDISEALTPLYPQLIERMESQGVTMTGPPMAYYLPAPKGPGDESITVHAAFPITGEVPADPGFAVVDLPGIERAATLLHHGPMSEAFRTGQAIARWIDDSGYQTVGTGFAREIYLDCPMDDLSKWITEMQVAVTPGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSP3</accession>
    <name>U5VSP3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PAS/PAC sensor hybrid histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_07715</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359363.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07715"/>
    <dbReference type="EnsemblBacteria" id="AGZ39832">
        <property type="protein sequence ID" value="AGZ39832"/>
        <property type="gene ID" value="AFR_07715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1574"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_51_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="153"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="397"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="515" mass="54980" checksum="45D65110CAB7C08C" modified="2014-01-22" version="1">MEWSEELSSGLLQAAPDAILVMDEGRIVLVNDRAVELFGWPRAELLGQHAQVLLTEAGRASYPDSMRRDPAGPEPEPGLMGTISVVVRRRDNTEFPAEASLARVQTPQGRLAVAVVRDMTERQQLQAEADLQRSEVQEHRNQRLEALGQLAGGIAHDFNNMLGVIVNYANFVIEEAESPEPDLKTIAADARQVIRAGERGTDLTHQLLKFARREVVRPVVLDLNSVITDVEELLRHSIGEHITLITRPAEVLPPVTCDPGQIEQLLVNLAVNARDAMPSGGNLVIDTACSRLEAGEHTDLAPGDYVRLRISDSGQGMPPDVVERAFEPFFTTKANGEGTGLGLATVYGIVTQAGGSVSLTSEPGLGTTVTVLLPAGAIEQEAAPVPAPVLTAGHGETLLVVEDEAALRDVAGRILSGAGYRVLAADGGSQALELAALHEGTIDLLVSDVVMPGMLGKELAERLTVVRPDTRVLYMSGYAQPVLASQGTLEPGVALLEKPFTAADLLSAVRRRLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSY2</accession>
    <name>U5VSY2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase GCN5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02335"/>
    <dbReference type="EnsemblBacteria" id="AGZ38756">
        <property type="protein sequence ID" value="AGZ38756"/>
        <property type="gene ID" value="AFR_02335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.472"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_28_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="15182" checksum="AA7D0B1F16586995" modified="2014-01-22" version="1">MRLSAFLAGPECHGVWTRRLGQVQLDPAEAAWVTGVIWDVDREIAVGRAGFHAKPDASGLVEIGYAVDPGQRRQGYARAALEVLLARAVAEPEVRRVRVSIRPDNIASFNLAMQYGFTKVGEQWDDEDGLEICYEVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT12</accession>
    <name>U5VT12_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359483.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08315"/>
    <dbReference type="EnsemblBacteria" id="AGZ39952">
        <property type="protein sequence ID" value="AGZ39952"/>
        <property type="gene ID" value="AFR_08315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1692"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039832_0_0_11"/>
    <dbReference type="OrthoDB" id="324612at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="466" mass="47957" checksum="C7A5184610D5B5B5" modified="2014-01-22" version="1">MSTSLDGLPVVVIGAGPVGLAAAAHLAERGITFTVLEAGDTAAAAVRQWAHVRIFSPWRYNIDSAAARLLTDAGWVRPDDDKLPTGGQLATDYLQPLADLAALKSSVRYNARVTAVTRLGLDRLRTAGRDTMPFLIRLADGEEVLARAVIDASGTWDTPNVLGASGIPAHGETASSSFLEHALPDVLGADRDRFAGKRVLVIGAGYSAANTLLLLAQSPGTDVTWAIRADTPARTYGGGSADALPARGALGTVLREHVEAGRINLVTGFAVQTLSPLPGGGVEVSDGTRTIAADRIVSATGFRPDHTITTELRLDLDPIMGAARTLAPLIDPNEHSCGTVPPHGAAELTHPEPDYYAIGAKSYGRAPTFLLATGYEQARSVVAALAGDWEAARDVQLDLPETGVCNSNVPVESDSIDGGCCGTPAAVEPAGRGLATGVAGGLLSTPLTLVSVSTPAEGEQAGGCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT38</accession>
    <name>U5VT38_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_08440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08440"/>
    <dbReference type="EnsemblBacteria" id="AGZ39977">
        <property type="protein sequence ID" value="AGZ39977"/>
        <property type="gene ID" value="AFR_08440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1718"/>
    <dbReference type="HOGENOM" id="CLU_1631824_0_0_11"/>
    <dbReference type="OrthoDB" id="2103991at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665179" evidence="4">
        <location>
            <begin position="27"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="162" mass="17670" checksum="EBFC93B034F2373A" modified="2014-01-22" version="1">MSKLLLIALVAATAATGALIITIAVGIPHPPPPPPGPNGMPPPPPNLSGLAAVSIVTGLFVLSWLAVLVVFSRDQILERLREVRHEPAPVHELDDMFAEFRSQLAADRDRERQLLEERITELTTEYGEQRETDGYLNGLRVATTEEPTSKVSSIRRVPRPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTK0</accession>
    <name>U5VTK0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DeoR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_10170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40323">
        <property type="protein sequence ID" value="AGZ40323"/>
        <property type="gene ID" value="AFR_10170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2072"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="475" mass="50793" checksum="2A164D59AD56833F" modified="2014-01-22" version="1">MARLLGQWHSLPGRHRSPDYAALAGAIRGLLTDSRLPLGVRLPAERELAESLGVSRTTVTAAYRALRETGHLTSRRGAGSWTTLPNGHRVASSGLWTPDDDMDMIDLGVAASAAPVELVPAARAAADDLPRFLGSAGYHPTGLMELREAVAEGYTARGVPTSPEQILITSGVQHALDLVLRLSVPAGASVLVESPTYPNALAALAARRARISTHGLDAGGGWDGEMLLGALRQTRPRLAYVIPEFQNPSGHLMHADLRERLVAAAHSTGTELVVDESFVDLPLDGTPVPPPVAVFDRHSRVVSIGGMSKAYWGGLRIGWVRASAPLVQRLAALRVGVDMASPVLEQLVAVHLLRDAETIVATRRAQLSIRRDALVAALGEFLPEWRFTVPTGGVMLWAELDGPISSALARAAEDVGVRLAPGPRFGLDGTLERFMRLPFALPADDLTEAVRRIASVRHDLDRASRPAWRSPAVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTR9</accession>
    <name>U5VTR9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_09565</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359849.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09565"/>
    <dbReference type="EnsemblBacteria" id="AGZ40202">
        <property type="protein sequence ID" value="AGZ40202"/>
        <property type="gene ID" value="AFR_09565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1950"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006909_8_3_11"/>
    <dbReference type="OrthoDB" id="324612at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="50320" checksum="629631069ADAC3CC" modified="2014-01-22" version="1">MQDTPDIADRHDAVCVIGAGGSGLAAIKNLREQGFSVDCYERETSVGGAWNWRHDRSPVYANTHLISSRPLTEFPDFPMPDDWPDYPHHSQLLGYLERYAKHFGLGDSIWFGTEVVAVTPVGDGRWDVTTRSTGGGAERIQRYAAVIVANGHNWSPKPLSVDTSAYRGKVMHASAYKDPAQLRGRKVLVIGGGNTGCDIAVEAAQQATTVWHSTRRGYWYAPKYIFGRPADQVNDKMLQLRLPLRLRQWLYHRTLRLTVGDVTRFGLPAPDHRPYESHPVVNSQLVYYLGHGRITPLPDVARLEGSTVELADGRRIEPDLVITATGYLPRFEFLAPELLDIDEDGKPDLHLHAFARKHPTLAVVGLVQADAGLFPLAHWQSVAVARWLRLRVSDPVRATAVQQKESTRPLRSWTRLRVVPSSRHWFEVSHTSYLRTLEDLLKQMEPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU93</accession>
    <name>U5VU93_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 6" type="primary">rplX</name>
        <name evidence="6" type="ORF">AFR_04560</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="3">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358187.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04560"/>
    <dbReference type="EnsemblBacteria" id="AGZ39201">
        <property type="protein sequence ID" value="AGZ39201"/>
        <property type="gene ID" value="AFR_04560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.921"/>
    <dbReference type="eggNOG" id="COG0198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093315_2_0_11"/>
    <dbReference type="OrthoDB" id="2040741at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_L24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="PTHR12903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="104" mass="11319" checksum="6B1BE3CD4BB758CE" modified="2014-01-22" version="1">MKIKKGDTVVVIAGKDKGAKGKVIAAFPRRDKVLVEGVNRIKKHERIRTTQRGSKTGGIVTQEAAIHISNVQLVDSEGNPTRVGYRIDDSGQKIRVARSTGKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUX3</accession>
    <name>U5VUX3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360696.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11800"/>
    <dbReference type="EnsemblBacteria" id="AGZ40649">
        <property type="protein sequence ID" value="AGZ40649"/>
        <property type="gene ID" value="AFR_11800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2403"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065219_0_0_11"/>
    <dbReference type="OrthoDB" id="1823496at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="136"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="32336" checksum="8C5CAFEC9F2FB58E" modified="2014-01-22" version="1">MDADVELVRDFNRYYTRRLGILTDRYLGQDRPLSEARLLFEIGDRADVRDLRARLGLDSGYLSRLLRSLEDQKLVRTHPHPDDRRVRVAELTASGVRECAELDRRSHAGISEMLEPLTADQRAELVAAQQQVRRLLRLAAVTIEAVPDDTDVARECLRAYAEELAVRFPEGYNNAALAKPGELAGFLVAREEGVAVGCGAWHRLGPATAEIRHLWVSSSARGLGLGRRLLTALEQDAGAQGITVARLGTHTSLTEAIALYRSSGYRQIARYGDSPYNQLAFEKAIAGPVPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUZ5</accession>
    <name>U5VUZ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359007.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05935"/>
    <dbReference type="EnsemblBacteria" id="AGZ39476">
        <property type="protein sequence ID" value="AGZ39476"/>
        <property type="gene ID" value="AFR_05935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1206"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050005_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="434" mass="45691" checksum="E7050E7E0C02E66E" modified="2014-01-22" version="1">MADAVPLIDLDVTPRAPAGTESLRPRITVPRPVLVLAVLIAALFVPASAVSPARTMRPVLSAGGTAAAAFTLGPDALYTASFGQQNPNSESGVRRYALPGGEQLWATSLAQNVQNLVLDGGILLARSGADARMSFLDINDGKVLWRLNDPGTSVVTMSRGRVLLRSDNGGTGTELRLVSARTGAPIWQRVLEGVGEIRNDDLFEDDPDRIVVLGVDGHVTTVQFADGSVLGEGDLGVRLPVELDGAFLADFVGITVSGDQLYMSRRDDGRASLTAFGLPDLTQKWRTEGGPAGFVHDCGPVLCISDTRWVSGLDPGSGAVRWSDPAWSNASPLSDGRLLAYGQQEIPESAILDARTGSVVRKLGQTYVTGGTELRADTRIPGRTWVNVTDSHAGDGAVHTVGFLETAAPYGCTVLAPYLACPTSAGPTTVWRIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVF3</accession>
    <name>U5VVF3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360846.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40787">
        <property type="protein sequence ID" value="AGZ40787"/>
        <property type="gene ID" value="AFR_12505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2540"/>
    <dbReference type="HOGENOM" id="CLU_1243145_0_0_11"/>
    <dbReference type="OrthoDB" id="1877572at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="222" mass="24899" checksum="7BF560EB13867CFD" modified="2014-01-22" version="1">MSYGALTVVGMATVVLLIVVLVVLYERSKQQRQRRELEGWAEQSGWTLTPKPREGWVRWLPRRVGGRPKVRNLVTGMVDGRRVSVGQYYALENDTWEPWIGVCAELRVALPTTTVWRVSPIGVLSPADRKTLGSTETELGHDEFDRRFRISTRQPETLQQWFGPHLTDAHLAGQMPTLWSVHDREVVVQMEGRLVPEEIAGLAAAVLPLVVLLEGSPSLSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVJ8</accession>
    <name>U5VVJ8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tad domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359182.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06810"/>
    <dbReference type="EnsemblBacteria" id="AGZ39651">
        <property type="protein sequence ID" value="AGZ39651"/>
        <property type="gene ID" value="AFR_06810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1391"/>
    <dbReference type="eggNOG" id="COG4655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_805691_0_0_11"/>
    <dbReference type="OrthoDB" id="1970501at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028087">
        <property type="entry name" value="Tad_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13400">
        <property type="entry name" value="Tad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Tad" evidence="2">
        <location>
            <begin position="13"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="372" mass="38400" checksum="084D4AA509D304A8" modified="2014-01-22" version="1">MRRLRQRGADDSGAIAVLVAILLSSGLLLGMSALVIDVGLLADEREQLQSGADSAAWGVAQACISEPADCAAQGQLAASLANANSRDERAGVTLDVCTDLCDLRPRDRISPCQDLAPGFAASYAEVRTSTETADGATVLPPRVAQGLIGGQNTGTQVNACSQVAWGLPGTLQVFGLGISVCDVQNADLYGVPGISPATRQHSGIVPGDLDPPAQGDDQTFVPLDLLSPVTCASAGLNLPQGFAWLGNTQNCSVTVARGDAAPIVGAPTAACRNALATIRNEHEPVLVPVYRGNPTLTSVRVVGFAVLVVTGHRGLIVPADQTSWLGGTTPTVLECLLQVCFSGYFSKMLVPRAQPEFVTGADYGVTVMGRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVK2</accession>
    <name>U5VVK2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peripla_BP_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360958.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13065"/>
    <dbReference type="EnsemblBacteria" id="AGZ40899">
        <property type="protein sequence ID" value="AGZ40899"/>
        <property type="gene ID" value="AFR_13065"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13065"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2653"/>
    <dbReference type="eggNOG" id="COG1879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_747282_0_0_11"/>
    <dbReference type="OrthoDB" id="1951491at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666124" description="Peripla_BP_4 domain-containing protein" evidence="1">
        <location>
            <begin position="33"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_4" evidence="2">
        <location>
            <begin position="74"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="370" mass="38890" checksum="C767717466EC2E33" modified="2014-01-22" version="1">MRTPTFALAGTALITTLALLTGCGGQEDQASAAIPAEAKQKAQALITPLTGKPGAFPITEPLKNRVPAGTRIAYVDNGNALVSLIYKLMQPAAAKLGVDLYAVRAGTSASGVQSAFSTVVQQRPAAVINTAIDPALWNNQRKELVAAGIPVISLGTVAGADSGLVQPNGEAAFERQGRLQAAFLVATNDKAVDAVLYVHNELAYGTAVRRGFEEGLKELCPDCSARVVQIPIAEVGNKAPERIVSDLRANTGTDAIAFQSSAVATGLPARLKVAGLEKIQIVGANPLPTNLQYLKDKQATVELGADLNTLAWTAIDTAARLITKQQPQQNVLDGEALVQQFLRPEDITFDPSRGFSAYPDVAERFAALWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW02</accession>
    <name>U5VW02_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chelated iron transport system membrane protein yfeD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_08080</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359436.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08080"/>
    <dbReference type="EnsemblBacteria" id="AGZ39905">
        <property type="protein sequence ID" value="AGZ39905"/>
        <property type="gene ID" value="AFR_08080"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08080"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1649"/>
    <dbReference type="eggNOG" id="COG1108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_3_1_11"/>
    <dbReference type="OrthoDB" id="1421296at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="293" mass="30255" checksum="E6DD9B4BBDCAD63E" modified="2014-01-22" version="1">MDLFQYDFMIRALTGALIIGLVAPALGIYLVQRRLSLIGDGIGHVALTGVGVGLLLHQSPVITAVIVSVIGAIGVELVRERGRTSGDLALALLFYGGIAGGVVLVGLSDDSSNANLMQFLFGSLTTTSRQDLTVIAVLGVAVLVAMLGLRQALFAVCNDEEYARVSGLPVRTLNLLMAVTTAVTVTIAMRTVGLLLVSALMVVPVAAAQQVTRGFRSTMVAAMLIGVGAAAAGITLAGEVNTAPGATIVILAIAVFVAITVGSAVWRILRRRVAAAHDHSEPPEVVLQQVGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWJ4</accession>
    <name>U5VWJ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359596.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08880"/>
    <dbReference type="EnsemblBacteria" id="AGZ40065">
        <property type="protein sequence ID" value="AGZ40065"/>
        <property type="gene ID" value="AFR_08880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1808"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033540_1_0_11"/>
    <dbReference type="OrthoDB" id="1759737at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="409" mass="44907" checksum="9223015D08D72B43" modified="2014-01-22" version="1">MDELPIGRRVAYWRGRRKMSQQVFADRLGKSKSWVDKVERGVRRLDKFSVVYEIADVLQIDVQLLLGKDVERRPETQNCIDQVEVEEIRAALERYDQMSAFFHPVPVAPPLTEMRKAVSHAWLTYQHAKYGVLARKLPQLLRDAQAADSAHASGEEARDAAHLLGQVYQIASSALRKVGEHELSWLAADRSIAVSQRAGDQLLAGMASHRVGMALLSLGRIRSALEVNVNIANRIAPMTSEAATPERLSVYGLLLLTGAMAAARIGDSATVRDLVCGAEQAAGALGGDYNYYWTSFGPTNVELHRAAAAVELGDGGKAIETHNRIDPESFAAMLPERRAHHFLDIARGYTQLGDVEKASEMLLEGDRLAPAEIRCRPLAHEVISDVLRRTRGTPPAPIAELAEQMGVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWP4</accession>
    <name>U5VWP4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359582.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08810"/>
    <dbReference type="EnsemblBacteria" id="AGZ40051">
        <property type="protein sequence ID" value="AGZ40051"/>
        <property type="gene ID" value="AFR_08810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1793"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="26377" checksum="55DA982B94FF7846" modified="2014-01-22" version="1">MSKVALVTGVGRRIGIAAAIAERLGNDGWDIAFNYWQPYDERMPWGRDAESRAELVRRLDRAGVRSLAVEADLEDAGAAEAIFERVGAELGAVGALVLSHAESVDSGLLDTTVESFDRHFAVNTRASWLLIREFGRRFTGEHGSGRVIALTSDHTVGNLPYGASKGALDRITLAAAHELAQLGITANVINPGPVDTGWMGPELIDTMVGLTPLGRLGTPADVANLAGFLCSPEGGWINGQLLYSNGGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWU7</accession>
    <name>U5VWU7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359815.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09480"/>
    <dbReference type="EnsemblBacteria" id="AGZ40185">
        <property type="protein sequence ID" value="AGZ40185"/>
        <property type="gene ID" value="AFR_09480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1933"/>
    <dbReference type="eggNOG" id="ENOG5031WV1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174732_0_0_11"/>
    <dbReference type="OrthoDB" id="2014538at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="73" mass="8132" checksum="A2A92BA84253C5EF" modified="2014-01-22" version="1">MIIDCGRCAERRDECNGCLVSTLYDTPDGIADLTPGELRAIETFELAGFEVELLETPDPPVDVPLYPRRRHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWW0</accession>
    <name>U5VWW0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="15">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">AFR_14975</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14 15">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="13">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="15"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361336.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14975"/>
    <dbReference type="EnsemblBacteria" id="AGZ41277">
        <property type="protein sequence ID" value="AGZ41277"/>
        <property type="gene ID" value="AFR_14975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3041"/>
    <dbReference type="eggNOG" id="COG1085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029960_0_0_11"/>
    <dbReference type="OrthoDB" id="1064872at2"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00608">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019779">
        <property type="entry name" value="GalP_UDPtransf1_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="PTHR11943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00117">
        <property type="entry name" value="GAL_P_UDP_TRANSF_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 15" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="6 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15 19" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GalP_UDP_transf" evidence="17">
        <location>
            <begin position="6"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="GalP_UDP_tr_C" evidence="18">
        <location>
            <begin position="182"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-alpha-D-glucose binding; shared with dimeric partner" evidence="11">
        <location>
            <begin position="24"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-alpha-D-glucose binding" evidence="11">
        <location>
            <begin position="73"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-alpha-D-glucose binding" evidence="11">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-alpha-D-glucose binding; shared with dimeric partner" evidence="11">
        <location>
            <begin position="308"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-alpha-D-glucose binding; shared with dimeric partner" evidence="11">
        <location>
            <begin position="313"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="10 14">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 14">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AGZ41277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="339" mass="37463" checksum="1A709E8C20755080" modified="2014-01-22" version="1">MTAPGAHRRLDQLTGEWVLVSPQRTARPWQGQVEDTSPAPRPDHDPDCYLCPGNTRAAGAVTPAYPSTFVFDNDFAALLPAPAGPGPGPDGLLVAEPVRGICRVVCFDPRHNVTLSGLEIPALRRVVNTWAAQTTELGSLPWVRHVQIFENRGAMMGASNPHPHGQIWAEDKIPGLPAKEIDRQRAYATTGGCLLCDYLSTELRAGRRIVCHNDEYVAVVPYWAVWPYETLVLPRRHGGALPDLDDAGREGFADILHRLTSGYDRLFGVEFPYSMGLHQQPTDGSPHPELHLHAHFYPPLLRSATIRKFMVGYELLAGPQRDITAEDAAERLRAVMKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWX3</accession>
    <name>U5VWX3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="2">P5C reductase</shortName>
            <shortName evidence="2">P5CR</shortName>
            <ecNumber evidence="2 3">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">proC</name>
        <name evidence="6" type="ORF">AFR_09630</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="2 3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359875.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09630"/>
    <dbReference type="EnsemblBacteria" id="AGZ40215">
        <property type="protein sequence ID" value="AGZ40215"/>
        <property type="gene ID" value="AFR_09630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1963"/>
    <dbReference type="eggNOG" id="COG0345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_3_1_11"/>
    <dbReference type="OrthoDB" id="1349288at2"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PTHR11645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0521">NADP</keyword>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 3" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="4">
        <location>
            <begin position="3"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="P5CR_dimer" evidence="5">
        <location>
            <begin position="159"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="27833" checksum="19123ED355A27922" modified="2014-01-22" version="1">MHTVAVIGAGKIGELVLSGLLRAGWPAEKLLATARRPERGAELQERYGVRLVDNATAVAEAGVLAIAVKPQDADALLAEIGAKIPADKLIISLCAGLPTSFFAARLPEGTPVVRVMTNTPALVDQAMTAISAGPHATGEHLAIAEEMFKPLGSTIRVPETQQDAVTALSGSGPAYFYLLVEAMIDAGILLGLPRQVAHELIVQTAIGSAVMLRDSGEHPVKLREAVTSPAGTTISAIRELENHGVRAALLSALEAARDRAREIAAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX24</accession>
    <name>U5VX24_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulatory protein degU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_09535</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359837.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09535"/>
    <dbReference type="EnsemblBacteria" id="AGZ40196">
        <property type="protein sequence ID" value="AGZ40196"/>
        <property type="gene ID" value="AFR_09535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1944"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="12"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="147"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="222" mass="23469" checksum="4F0E1EE44935F6D2" modified="2014-01-22" version="1">MSTSPMPTTRTKVLLVDDHDLIRKGLRHAFERDRQFEVVGEAATAAEAVRQAGALQPDVVIMDLRLPDGSGLEATRALRKNNANMGIVVLTMYAGDDQLFGALEAGASAFVPKTAPADEVVAAARHAASAPSAFTAADLAEAMKRRLAPSGPQLSPREGQVLRLLADGMSVAGIAKQLFVSESTAKTHISKLYEKLGAANRAQALMTALRLGLLEAPDAPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXI5</accession>
    <name>U5VXI5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">YbiT-like ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_10780</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360328.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10780"/>
    <dbReference type="EnsemblBacteria" id="AGZ40445">
        <property type="protein sequence ID" value="AGZ40445"/>
        <property type="gene ID" value="AFR_10780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2197"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_11"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="328"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="360"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="534" mass="57785" checksum="D9CBFEA62EBAD211" modified="2014-01-22" version="1">MITATGLELRAGSRILISPTTLRVQPGDRIGLVGRNGAGKTTTLKVLAGEGTPYAGEVTRTSEVGYLPQDPRTGDLNVTARDRVLSARGLDVLLSEMQKLEIELEESADEKLIRKYGQLEDQFASLGGYAAEAEAARICSNLGLPDRALAQTIGTLSGGQRRRIELARILFQNSGQNGKGILLLDEPTNHLDQDSITWLRGYISQHKGGLIVISHDVDLLEATVNRVWYLDANRAEVDMYNVGWKTYLAQRETDERRRRRERANAEKKAGALLAQADKMRAKATKTIAAQNMAKRANKLLDGLEDVRTSDRVAKVRFPSPAPCGKTPLTAHGLSKSYGSLEIFADVDVAVDRGSRVAILGLNGAGKTTLLRILGGHLQSDTGEVRPGHGLRLGYYAQEHETLDVDRTILDHMRSAGGEQTDTELRKILGAFLFSGDDVDKPAGVLSGGEKTRLALATLVCSGANVLLLDEPTNNLDPISREQVLDAIANYPGAIVLVTHDAGAVQALKPDRAILLPDGDEDAWSDDLLELVELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXP1</accession>
    <name>U5VXP1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Group 1 glycosyl transferase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_17350</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361970.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17350"/>
    <dbReference type="EnsemblBacteria" id="AGZ41748">
        <property type="protein sequence ID" value="AGZ41748"/>
        <property type="gene ID" value="AFR_17350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3518"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042257_1_0_11"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="18"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="186"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="378" mass="40238" checksum="460F3E74FC9F3088" modified="2014-01-22" version="1">MRVLVNAGPWLQVPPHGYGGIENVIATLVPELRRLGVEVVLATVGESSLPVEEQVSVFPEGMFGVLQRPYNQVCGVVPAHQNAVVRRLAEGDIDLVHDHVEAAGLSTLVALGAYGPPALHTLHWDLQKHPELYGTFDGGDRVRVNGVSADQLTHAPAALQAGSVGHVHLATPLAVGADRRAPVVKDGSVVVLGRINPGKGQDLAARLAHEAGFDLVLAGPVGPYHRPEDLAAADQQNPDVRFWREHVAPHVDGDRVRWIGTVTGEDRDQLVAGARATLFPLRWNEPGGTAVVESLALGTPVVGIARGCLPELIEHGRTGLLTTDEAELGDLVQAVDKIEEAECRAEAARRFTPAVMAAGYLELYEKVRSLAAGSTERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXP5</accession>
    <name>U5VXP5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_17375</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361980.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17375"/>
    <dbReference type="EnsemblBacteria" id="AGZ41753">
        <property type="protein sequence ID" value="AGZ41753"/>
        <property type="gene ID" value="AFR_17375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17375"/>
    <dbReference type="eggNOG" id="COG1835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005679_2_0_11"/>
    <dbReference type="OrthoDB" id="1979131at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl_transf_3" evidence="3">
        <location>
            <begin position="13"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="431" mass="46556" checksum="B4324E49CBBF5EA6" modified="2014-01-22" version="1">MPATVPAPGERIAWLDGLRALAVLLVVYAHLSRYLFRGARDFSSEWLHAGTAGVMLFFLVSGFIIPASLERHGSLRAFWVSRLFRLLPLYLVVTAVVVVLGLGGLVPLDPWLTAHPWTSAVAHATMLPHLLGVPLVTPVFWTLTFEMVFYLLVSALFAVRWHRRGGAVAVILAVLTVCTVPLAPTLIPTGTATVVAAVSLLIGLVAVTSGHRWAVVPGGLLLAALAGTLLVADQDPAHVWDGLLIVAVMFVGTTLYRAQQRQLRWWAALTVAATVAAALLFTWYAELDALGAPTARYRTRSVITLVVFGGAFALALLTRHRRTPRALAWLGVVSYSVYLVHFVLIQLLAPVLTGLGNRLPAIAEVPVAVAFLALLLSLSWLTHRFVELPGQRLGRRLARWLTARRGSDALISDPRPRSPVPAGVPPPTGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXU5</accession>
    <name>U5VXU5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA polymerase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_17675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362099.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17675"/>
    <dbReference type="EnsemblBacteria" id="AGZ41813">
        <property type="protein sequence ID" value="AGZ41813"/>
        <property type="gene ID" value="AFR_17675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3583"/>
    <dbReference type="eggNOG" id="COG0258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004675_1_3_11"/>
    <dbReference type="OrthoDB" id="1220182at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038969">
        <property type="entry name" value="FEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42646">
        <property type="entry name" value="PTHR42646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="53EXOc" evidence="2">
        <location>
            <begin position="5"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="340" mass="36478" checksum="CA858584DCB97513" modified="2014-01-22" version="1">MPAPAPLLLVLDGNSLVHRAYHAAALDGLLDKAGRPVWALKGLIGYIARAAAHLRPSGVVVGFDCPDDSARKVDYPAYKAHRPAKSADLAEQIAGAPELLRAAGICTVVPPSYEADDVLASSAEHARSRGWRSVLMTSDRDAFALIDDTTSVLRVRNGGFDEAVLIDAPALVELYGVHSWQYRDYAALRGDPSDNLHGVRGFGRARAAALLKTFGTVDAAWAAIDRGDSAAVREVVGEQALQHLAAAATRDIVARNRRLMAMRADLPMPDLSAARLPLRRSVIRWALSGRGIHLGPSLWALTGESAPVGTEDLTPELRPWVFRRGLASSREPTPGQLSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYH3</accession>
    <name>U5VYH3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative LacI-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_18305</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362312.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18305"/>
    <dbReference type="EnsemblBacteria" id="AGZ41939">
        <property type="protein sequence ID" value="AGZ41939"/>
        <property type="gene ID" value="AFR_18305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3715"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_4_11"/>
    <dbReference type="OrthoDB" id="664909at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="344" mass="36012" checksum="D0BB94667AECB18A" modified="2014-01-22" version="1">MPKPGPRLRLVDVAERAGVSLATASRALAGRDGVSEEVATRIREISRELGYVANPYARTLAGGASSTVGLVVHQVDDPYFSEIAGGVIQVADEQSLLVQICHSGRDPDHELRQIRHLIAQRVGIIIIAGSGYNDPRIEAEAKAELSAFQSLGGRVAVIGRHSLGVDAVLPDNEAGGQALGDHLVGLGHRHIAVAAGTPGLTTVADRLAGVAAGLRAHGLSLDDLPVVHTDFTRDGGRAAADQIMTNHPATTAIVALNDAMAMGVLSALRARRTPVPDRVSVVGFDDVSVAADLAPSLTTVRLPMTDMGRMALELALKPRSARPRRRATGHRLIVRDSTGPVPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ04</accession>
    <name>U5VZ04_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sirohydrochlorin ferrochelatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362385.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18670"/>
    <dbReference type="EnsemblBacteria" id="AGZ42012">
        <property type="protein sequence ID" value="AGZ42012"/>
        <property type="gene ID" value="AFR_18670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3790"/>
    <dbReference type="eggNOG" id="COG2138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056929_1_0_11"/>
    <dbReference type="OrthoDB" id="1573073at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="23271" checksum="AAE0ABE7F7E60395" modified="2014-01-22" version="1">MSRPPALLAVAHGTRSAAGQAQIRDLVDRVACRRPELDVRLAYVDVQQPRVADALHGPDTVVVPLLLSAGYHVRVDIAEAVAAHHAVAAPPLGPDPLLVESLRRRIGPGDAVVLAAAGSSDPVWRADIAGVAAQLGAHVAYASGADLRVPDVIARLRAEGARHITVAAYLLAEGLFYRSLHRAGADVVTPPLCEDPSLVDLVLSRYDRQTADLTTAIRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ09</accession>
    <name>U5VZ09_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362580.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19680"/>
    <dbReference type="EnsemblBacteria" id="AGZ42208">
        <property type="protein sequence ID" value="AGZ42208"/>
        <property type="gene ID" value="AFR_19680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3994"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142884_0_0_11"/>
    <dbReference type="OrthoDB" id="2047944at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011944">
        <property type="entry name" value="Steroid_delta5-4_isomerase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13474">
        <property type="entry name" value="SnoaL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02246">
        <property type="entry name" value="TIGR02246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="120" mass="13551" checksum="A20A3BC7AC88C65E" modified="2014-01-22" version="1">MDFTAAVNLHLTTIGKRDLDGYLSTVHDEVSLIMPNGKLLSGREEVAAFHRGWFDDPDWSWELDLQRSTTAGDTGVAIFAVDYHDLDGNGQPYELSYLLTLVFVRNGDTWLLLHDQNTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZD5</accession>
    <name>U5VZD5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_20430</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20430"/>
    <dbReference type="EnsemblBacteria" id="AGZ42358">
        <property type="protein sequence ID" value="AGZ42358"/>
        <property type="gene ID" value="AFR_20430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4143"/>
    <dbReference type="eggNOG" id="COG2011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077375_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="199" mass="20583" checksum="3F84C7B21520CB53" modified="2014-01-22" version="1">MVGVSTLLTAVGGLLLGILLVLTDRGGLLSAPPVNALLGLIVNVGRSLPFIILLVAVIPFTRAVVGTTIGTTAAIVPLTIGAIPFYARIVETALREVPPDVVSAAVAMGASRRQIIGKVLLREARPGLVAGLTITVIALVGYSAMAGVVGGGGLGDLAIRYGYQRFETDVMIATVVVLIVFVQLIQTLGDVLVRRLSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZD7</accession>
    <name>U5VZD7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361119.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13870"/>
    <dbReference type="EnsemblBacteria" id="AGZ41060">
        <property type="protein sequence ID" value="AGZ41060"/>
        <property type="gene ID" value="AFR_13870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13870"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069129_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="31"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="27962" checksum="407B6AB35EB83C1B" modified="2014-01-22" version="1">MEEFPSLYDLTYADYQEDIPFWIDLIGRTEVLELGVGTGRVALPLAVAGAGVTGLDHSLQMIRSARNKMTQAGSRINLVYSDLTRLPFSEGSFASVICPFSTINYVTDLAALRRAFSEARRVLRKGGLFAFEALSWHTYDRWLNNDGLQHVVDRVGKAPGEPIDITYSYQFDAAHQIATQERLYRVTVADGVRKEYLIHWRNRFAFRAEYLLLLEDSRFAIELEAGDYQGSTYSHDAEAYIVVCRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZG4</accession>
    <name>U5VZG4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative oligopeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_19450</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362534.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19450"/>
    <dbReference type="EnsemblBacteria" id="AGZ42162">
        <property type="protein sequence ID" value="AGZ42162"/>
        <property type="gene ID" value="AFR_19450"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19450"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3947"/>
    <dbReference type="eggNOG" id="COG3104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004790_0_1_11"/>
    <dbReference type="OrthoDB" id="470322at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17346">
        <property type="entry name" value="MFS_DtpA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005279">
        <property type="entry name" value="Dipep/tripep_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="PTHR11654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00924">
        <property type="entry name" value="yjdL_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="9">
        <location>
            <begin position="1"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ42162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="485" mass="51319" checksum="7E8A730638C8DD8E" modified="2014-01-22" version="1">MTTPTRERTFFGQPPVLANLFGVELWERFSFYGMQGILLIYLYYSAADGGLGLNEQTATSIVGAYGGAVYLSTILGAWAADRLLGAERVLFLSAVLVMAGHISLAVLPGLTGVTTGLILIAIGSGGVKANATSLVGSLYDEHDEHDERRDAGFSLFYLGINLGALAGPLLTGLLQKTWGFHAGFGLAAAGMAAGLVQYTLGRRRLPREADEVPNPLPAGRRLPVAVIVAAVAVLIVVLAVTGVLRADRLSDVVIVLSALAAIAYFVVILSSRRITGVERRRVLAFIPMFVASAVFWSLYQQQFTVVTIYSDRRLDRDLFGWEMPVSWVQSINPVFVIILSGVFAALWTRLGPRQPSTPVKFAAGTAIMGVAFLLFLPLAGGGPNSAPLLGLAGILLVFTIAELLLSPVGLSLSTKLAPRVFHTQMVALFFLSVALGTAASGTLAGYYDEAHEVAYFGVLGAVAIVIGAALALAARPISRLMSGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0F3</accession>
    <name>U5W0F3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361470.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15645"/>
    <dbReference type="EnsemblBacteria" id="AGZ41411">
        <property type="protein sequence ID" value="AGZ41411"/>
        <property type="gene ID" value="AFR_15645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3177"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="25154" checksum="B8AE241308168CAC" modified="2014-01-22" version="1">MELTAVVTGAARGIGRSVVEQLRAKGYRVVAVDVSPAVEELAEAGRVVPMVADVTGEATPGDVIGRATAEFGRLDLLVNNAARFLRRPILATSDADFDALFALNVRPVFRFVKAALPALTETRGCIVNTASISGLIGVPNQSVYAMTKGAIVQLTRQLAIEYAGRGVRVNAVAPGAVDTAFMDEAREADPDPAASLAATLANHPIGRMSTPGEVARAIVFLAAPESGGITGTILSVDGGYVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0K5</accession>
    <name>U5W0K5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-erythro-3,5-diaminohexanoate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363005.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21820"/>
    <dbReference type="EnsemblBacteria" id="AGZ42634">
        <property type="protein sequence ID" value="AGZ42634"/>
        <property type="gene ID" value="AFR_21820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4421"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_826176_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="35387" checksum="0501EA03BE72EFBD" modified="2014-01-22" version="1">MTFPVGLSRVLEPAGVLPQAAWRLDARPELGPDEVRIRVERLNLDAASFRQLATKHGGDGAAARSEVLEIIGTRGKMHNPVTGSGGMLIGVVDEVGPESPLPVKAGDRVATLVSLTLTPLVVSDGLAGWDGLSEQVPAAGHAILFGRSVVAVLPDDLSPELALAVFDVCGAPALTDRIVKAYVGAAAIAHDAEPVTVAVLGGAGKSGSLALTAARLAGARTVGVVVSDAEREALQLAGLADHVAVADARDPVAVSSAVVDALGGAATVTVVCVDVPGCEHGAILATASGGTVIFFSMATSFPAAALGAEGLAADVTMLVGNGYVPGHAAFALDVVRRTPAVRALFEARIAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0R3</accession>
    <name>U5W0R3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4240 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363340.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22840"/>
    <dbReference type="EnsemblBacteria" id="AGZ42838">
        <property type="protein sequence ID" value="AGZ42838"/>
        <property type="gene ID" value="AFR_22840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4629"/>
    <dbReference type="eggNOG" id="ENOG5032D76">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1486025_0_0_11"/>
    <dbReference type="OrthoDB" id="2016600at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025334">
        <property type="entry name" value="DUF4240"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14024">
        <property type="entry name" value="DUF4240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4240" evidence="2">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="181" mass="20511" checksum="D9C100839538176F" modified="2014-01-22" version="1">MDRAVFWRIVEGARAEAGTDTERVAQVLLRRLRSLSPDEIEHFGALWAEVQDELYSWPVHDAATLLLGPVNDDALLVVQDWIVSHGRQTMKRVQEDPDNLVELAPDRHNARIDWFTGLPMEAHIAATGRPFAVDGPDGPEDPTGTPTDLTDEPGTRRRFPRLTAYLDHNTWIERPWTRATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>U5W128</accession>
    <name>U5W128_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Winged helix family two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_23465</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363552.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23465"/>
    <dbReference type="EnsemblBacteria" id="AGZ42963">
        <property type="protein sequence ID" value="AGZ42963"/>
        <property type="gene ID" value="AFR_23465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4755"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_11"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="129"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="129"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="232" mass="25662" checksum="7E858E3F7EABEEE8" modified="2014-01-22" version="1">MSYLLVVDDDPTVSDVVRRYLEQDGHSVRLAADGAEALAAAAAYPPDLAVLDLMMPGIDGIEVCRRLRTRWPHLPVVMLTALGEEADRVLGLEVGADDYVTKPFSPRELVLRIRSVLRRTAPATADAGPAVLHDAGLSADVRRRIAHLHGNQLALTVREFDLLAFLLQHPARAWSRAELLEKVWGWQFGDQSTVTVHVRRLREKIEQDPAAPRRILTVWGVGYSYEPAGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W163</accession>
    <name>U5W163_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Histidinol-phosphatase (PHP family) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23070"/>
    <dbReference type="EnsemblBacteria" id="AGZ42884">
        <property type="protein sequence ID" value="AGZ42884"/>
        <property type="gene ID" value="AFR_23070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4675"/>
    <dbReference type="eggNOG" id="COG1387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054611_3_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHP" evidence="1">
        <location>
            <begin position="3"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="262" mass="28993" checksum="CF5733A2E7DD398A" modified="2014-01-22" version="1">MGDMEQSCARAVELGLPAIAFTEHLDHTVWRIDADSPYRSDHLAALLDADGLLTPPPFDAAGYLAAVERCRERFPQLRIHSGLELGEPHRHAGQIARVLAAGRFDRLLGSLHTLPDQGAFAEPWGIYPHRDADEVVRAYLADVVTMVSTSELFSVLAHIDYPVRSWPAGAGPFDPARFEEEFRWALRATAQSGRALEVNTRLPLHATVLTWWHQEGGDAITFGSDAHLPALVGHGFREAADLAEAHGFRPGLNPYDLWRRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1K8</accession>
    <name>U5W1K8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heavy metal translocating P-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_23185</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363422.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23185"/>
    <dbReference type="EnsemblBacteria" id="AGZ42907">
        <property type="protein sequence ID" value="AGZ42907"/>
        <property type="gene ID" value="AFR_23185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4700"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_11_2_11"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="618"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="647"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="674" mass="70510" checksum="E43A32659187C506" modified="2014-01-22" version="1">MDAAHDHHAGHDKHAGHDPDLFRRKFWLSLALTVPIVVTSHMVMDWFGYSLSFPGLEAVGPVLGTVVFFYGGWPFLAGAVTEVRDRAPGMMLLISMAITVAYAASLATSFGAFDLDFWWELAALVTVMLLGHWQEMKAIGQAQGALAALAALLPDEADRIEDGDTVRVRVADLRVGDTVLVRSGARVPADGRIVDGTAELDESMITGESRPVERSTGDRVVAGTVATDSALRIRVDAVGDDTALAGIGRLVAQAQAAGGRAQVLADRFAAMLFFIATAAAALTFVVWWLLGDLDGAVVRTVTVLVIACPHALGLAIPLVIALSTAVAAKAGILVKDRLALERMRTVDAVLFDKTGTLTRGAPTVTGVAAGGGRTEEEVLGVAAAVEADSEHPLARAIVAAAHRRELRLSATDFRSLTGRGVRAAVDGTTYAVGGPSLLRELDAEVPGELARAAEEWSRRGAAVLHLLELTDSRPEVIGAFALEDEIRPEARAAITQLREQGVAKIVMITGDARPVAEAVAAQLGFRPGTDEVFAEVLPADKDRAVADLQARGLTVAMVGDGVNDAPALARADIGIAIGAGTDVAIESAGVVLASSDPRAVTGVIRLSKASWRKMIQNLGWAAGYNVVAIPLAAGVLAWTGFTLSPAVGAILMSASTIVVALNAQLLRRVRLVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1U1</accession>
    <name>U5W1U1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="10">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_23980</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363654.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23980"/>
    <dbReference type="EnsemblBacteria" id="AGZ43064">
        <property type="protein sequence ID" value="AGZ43064"/>
        <property type="gene ID" value="AFR_23980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4858"/>
    <dbReference type="eggNOG" id="COG0058">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010198_1_1_11"/>
    <dbReference type="OrthoDB" id="137158at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102250">
        <property type="term" value="F:linear malto-oligosaccharide phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102499">
        <property type="term" value="F:SHG alpha-glucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="PTHR11468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ43064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="808" mass="90932" checksum="40A9D24ADA218542" modified="2014-01-22" version="1">MTYVTPHPSNTPGDFGRDLLSNLYYQRGTTLESASAQDAYHSLALTVRDRLVDRYIRTAAAHYQANPRFIYYLSAEYMLGKQLDQNLLYSGTADCARSALAGLGLSPAELDALDVEPGLGNGGLGRLAACLLDAMATLDIPAVGYGIRYDFGIFKQAFEDGAQVERPDDWTFYGNPWEFHASDDQQTVGFYGHTEPLDGVRKSWVPGEIVMGEPSHMLVPGYATSTVNIIRLWRARASRESFDLSRFGAGQYGEAVEEIVRSENISKVLYPDDSTELGRELRLKQQHFLVTCALRDIVRRFRLRNSDWDDFAGKVVIQLNDTHPVMAIAELMRMLVDEEGLEWDHAWSITRRVFAYTCHTLLPEALETWPVRLVQRLLPRHMEIIFLINHLFLREVSARYPGDDGKLRELSIIAEGDEQRVRMAHLAVVGSSAVNGVAELHSRLLAATTLHGFAELWPGRFHNVTNGVSPRRFVRLANPRLSELITERLGDDRWLSDLDRLGGLESAADDPEFQERWRQVKRLNRQDLADQALATTGVALDPDALCDVMIKRFHEYKRQLLRVLHVVVMHHRIRRGEVADVVPRSIVFGGKAAPGYYAAKRIMQLANTVAGMVNEDPAVSPYLKMIFLPDYNVSRAQLIIPAADLSEQISLAGKEASGTSNMKLALNGALTIGTLDGANVEIRDRVGAENFFLFGLDTAEVAAVREKGYRSRDFYERDEELREVVDTVARLEPQIADDLLNHDEYLTLADFRAYVDCQDRVARAWRDTAGWTRASILNTARSGFFSSDRTVRDYCRDIWHVTPVPVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2E6</accession>
    <name>U5W2E6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_24605</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tannase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363777.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24605"/>
    <dbReference type="EnsemblBacteria" id="AGZ43187">
        <property type="protein sequence ID" value="AGZ43187"/>
        <property type="gene ID" value="AFR_24605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4985"/>
    <dbReference type="eggNOG" id="COG0627">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014819_4_1_11"/>
    <dbReference type="OrthoDB" id="865015at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011118">
        <property type="entry name" value="Tannase/feruloyl_esterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33938">
        <property type="entry name" value="PTHR33938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07519">
        <property type="entry name" value="Tannase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666004" evidence="6">
        <location>
            <begin position="19"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="498" mass="52727" checksum="6241DB465DA7AF20" modified="2014-01-22" version="1">MIKLLLAALIAVPVPAPAPSAPVTGCAALSRMDLPGVRITSAALALTRTNEFCQVEGHFSPGTSFTIKLPPSGWTGQYVQHGCSGLCGEVPALDVPVGGFQCEDAINGKLVLAASDTGHQGDGPATWGYDPRKRIEFGLTSEHKLRNVATAVMTSYYGRGPSHRYFTGCSTGGRQALNLAQRYPADFDGILAGAPANNLSALSGLLNPWLVVKNTGPGGRPILPVSKLPALHSAVARACGDAITDPRQCAFQPSSLRCPPGQDKPTCLTGTQVAAVTAFYRGPTDSQGRSLYNGGVPYGSELSWTWFLGPAAGDIALNYLRYLAYPHNPPASFDLGDVKFTKAAFDRLSVVGDAIYNANNPDLSAFRAHGGKIILWHGWADASIPPWSTIDYYRAVERRAGGFKASQSFSRLYLVPAGYHCLFGPADPPTEIGAPEFLTPLMTWVEGGDAPDVVGVPTIAPDLETLIRYLEVSPHDALAPVAAAPGSLNSGYRYVGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2K7</accession>
    <name>U5W2K7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24905"/>
    <dbReference type="EnsemblBacteria" id="AGZ43247">
        <property type="protein sequence ID" value="AGZ43247"/>
        <property type="gene ID" value="AFR_24905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5045"/>
    <dbReference type="eggNOG" id="COG4279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053146_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="26467" checksum="CE1200B9CCA69E42" modified="2014-01-22" version="1">MTDRYSGPFIAMFESLRMAPTFSRGRRDARAGHVRHLTISGSLVTAQVRGPDDTAAFRSRIAVRAFGAAEWSRVEDDLAAEARFAADLLAGRMPDDIDTVFTKAGLTLLPLSLNEVAMDCSCERWPMPCIHLAATCYALARSFEEDPFGVFAWRGRSRDELLTRLNQLRGVAAPATPAEETPDGDLGDLTDFWNGAEAAPATAVGDAGRPDLLLDQLDPPPLHVGGVPLVEVLRPAYHALPPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2M7</accession>
    <name>U5W2M7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AP_endonuc_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26105"/>
    <dbReference type="EnsemblBacteria" id="AGZ43483">
        <property type="protein sequence ID" value="AGZ43483"/>
        <property type="gene ID" value="AFR_26105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5291"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1022606_0_0_11"/>
    <dbReference type="OrthoDB" id="1007505at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="69"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="27154" checksum="359A3816B8A540EC" modified="2014-01-22" version="1">MTKPFGELEPGRLAEILGGMSADGGEVVVRDGQTVTPGEPQRLLEVAKEFARHGLGIDVVTTDLVRADATADRVFGVCAEAGVPLVRVGWWRYDAGLGYQRIAAEARRELAALAALARRHHLRIALQLHHGTIHPSAAHALRLTEELAVYADPGNQVMEGREDLAMSLDLLGDRVACVGVKNAAWLGERLGWQPLADGGVLEWPDILRTLHQRGYDGPLSLHAHYPMTDPGATVARDLRHLREAISLSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2P7</accession>
    <name>U5W2P7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_19545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19545"/>
    <dbReference type="EnsemblBacteria" id="AGZ42181">
        <property type="protein sequence ID" value="AGZ42181"/>
        <property type="gene ID" value="AFR_19545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3966"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061724_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="30604" checksum="7AFC5E107550F8EC" modified="2014-01-22" version="1">MAVMTDVTPALAREAWRLAEPLHAMIYFVPEAQERYDALGIPVTAGYFASRGGVFGPVGPGPVVATFYNFEPGLVARALPAVWEIASPAAALAARLDAAGAALTRGLGDAVKSPEVAEAAELARRAAESATAYAEGRPLFAAHAGLDWPDEPHLKLWHAITLLREFRGDGHVAALLVAGVTGLQSIVLHAASGQANGRFLRASRGWSRERWAAEVAVLQERGLIDGEELSESGRELRARLEADTDRLAVPAYQVLGSEGCERLAELTRPFSRTLVKAGFLAAAAPPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W336</accession>
    <name>U5W336_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hemerythrin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_20345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362713.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20345"/>
    <dbReference type="EnsemblBacteria" id="AGZ42341">
        <property type="protein sequence ID" value="AGZ42341"/>
        <property type="gene ID" value="AFR_20345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4125"/>
    <dbReference type="eggNOG" id="COG5592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079417_4_0_11"/>
    <dbReference type="OrthoDB" id="1701106at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin" evidence="2">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="167" mass="19036" checksum="9A995865ED1C1810" modified="2014-01-22" version="1">MDDITALIMEDHHRFRVGFARLDDAEGAGQLAAIWEPLALHLDIHADAEEEIFYPHLLKEAGGEDAEEETDDAIGDHNKIRDGIEEARKHPVGSDGWWAGVWTARKENSEHLMEEEDEVLPDFRKHATPQLRADLAVEWLRFFADHPQGEGLDFSDKDPKRYIAENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3A2</accession>
    <name>U5W3A2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364417.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26585"/>
    <dbReference type="EnsemblBacteria" id="AGZ43579">
        <property type="protein sequence ID" value="AGZ43579"/>
        <property type="gene ID" value="AFR_26585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5388"/>
    <dbReference type="HOGENOM" id="CLU_147818_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045629">
        <property type="entry name" value="DUF6232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19744">
        <property type="entry name" value="DUF6232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="135" mass="14792" checksum="B26072FA74979C58" modified="2014-01-22" version="1">MRVFYRGPGAVITAELVTVVHSVRRSYVLAELEHVHIVRQQGRNGLADHRAMGVSALVSGIIVVPVVGQVSALLAAVVIIVLVLGAVVYMRLGPPAHHELVATYRGRRVVVFASDNEREFDQVCRGFQRAKEQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3K8</accession>
    <name>U5W3K8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_21270</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362898.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21270"/>
    <dbReference type="EnsemblBacteria" id="AGZ42526">
        <property type="protein sequence ID" value="AGZ42526"/>
        <property type="gene ID" value="AFR_21270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4314"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_5_0_11"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="9">
        <location>
            <begin position="22"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ42526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="261" mass="28032" checksum="35435B1047A98E6F" modified="2014-01-22" version="1">MTTQTMPVTRNLPRFGGFTLGMIRLELRRLMRNRRTVIFTLVMPAVFFLLFGTNASSRSERVGGGNVAGYILVSMAVYGAMLATTSGGAMVSIERAAGWSRQLRLTPLRPVAYIAVKLVVAMTIGAVSVVVAFAVGAVAGAELPVHAWIECGLLAWVCALVFAAFGLFMGYLLPSENVMQILGPVLAVLSFAGGLFVPVDQLGHTFATIAKFTPVYGVGEIARHPLTHDGNLWLAALNVVVWTAVFAGGAMWRFRRDTARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W478</accession>
    <name>U5W478_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560355.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28785"/>
    <dbReference type="EnsemblBacteria" id="AGZ44018">
        <property type="protein sequence ID" value="AGZ44018"/>
        <property type="gene ID" value="AFR_28785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5835"/>
    <dbReference type="eggNOG" id="ENOG50328FR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2523861_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="74" mass="7293" checksum="56A8151FFCDD69DA" modified="2014-01-22" version="1">MTTQHGNRDGELPIDPAAKPEQGAPLTQVETDRRPPGVADSDIVTEPDGSGTAGGASGSTGGGSSMPGHPDAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W530</accession>
    <name>U5W530_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadK</name>
        <name evidence="6" type="synonym">ppnK</name>
        <name evidence="6" type="ORF">AFR_29940</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29940"/>
    <dbReference type="EnsemblBacteria" id="AGZ44249">
        <property type="protein sequence ID" value="AGZ44249"/>
        <property type="gene ID" value="AFR_29940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6064"/>
    <dbReference type="eggNOG" id="COG0061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="65"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="180"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="30927" checksum="8A96072643CD1854" modified="2014-01-22" version="1">MVTHTGRRQSTQHAQTVARDLVEAGFEVRVIAQEVADLELPAGVTPVDDPTAAEGAEIVIALGGDGTFLRAAELARPAKTPLLGVNLGKVGFLAEAELSTIDQTVRDIVAGDYTVDERLTLDVRAEYDGRLIADSWALNEVSVEKGQRAQMLELLVDVDGRPLSRYGCDGVVCATPTGSTAYAFSGGGPVVWPEVEALLLVPISAHALFSKPLVTAPTSVFVLTVDPYTSFAVLCCDGRRTWDLPPGAQVTVQRGELPVRLVRLAPRPFTDTLVAKFGLPVDGWRGNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5B2</accession>
    <name>U5W5B2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative diguanylate cyclase/phosphodiesterase with PAS sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24520</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363760.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24520"/>
    <dbReference type="EnsemblBacteria" id="AGZ43170">
        <property type="protein sequence ID" value="AGZ43170"/>
        <property type="gene ID" value="AFR_24520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4966"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="445"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="600"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="3">
        <location>
            <begin position="746"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1005" mass="108330" checksum="1DA29FC6FCF3696C" modified="2014-01-22" version="1">MTTRSARACFAAWSLVLTAVYYAFPGLHVWTWGLIGLSSAAAVVVGARMKRPARALPWYLIAVALVVFVSGDTIYYAQQSLGLETPFPGPSDVLYLLVYPLLAGAFLIFIRARSGGANRAALLDALVPTVSLGLLSWVYMIAPYTRATDLTLLEKAVSVAYPLGDVLALAMMLRLLTAPGRKPRALSFLSLSIVGVLVSDVVYGLARLDSDWNVGGPIDLGWIVFYAAAGYAALHPSMTHLTDDAPQTAGTVETGGRRLLLMTFAALIAPAVLLAQYLQGDVVDAPVIAAGSALMFLLVMGRVGGLLTEQRLTNLRESALRRANADLAAATTETDILTTIRTAIAQLMPVQQRHQLVVDEPVPLDLAATVELGVSLVPASEHGFDVALRAGMVLPGSPPKLGSIHLFAPADVLRSVRPAVQALFDQITMVVERIGLAGEISRRDGEAYFRTLIQSASDVILIVGDDDRIRYASPSATTVFGWTDLPGKPLSGLIADTHHGALAEALMQARSGRGQLEAVDLTAVCADRRLLQVECAGRDLRHDSTVSGLVLTVRDVTERRRLENDLAHQAFHDGLTGLANRVLFRDRLEQSFVRSEQGSSDVGVLFVDLDDFKEVNDTLGHAVGDQLLIAVGRRIADAIGVANTAARMGGDEFAILVESSSDPAVAEEVAERIVNVLSQPVEVSDGLGGSHIVSGAASVGVATSHDATSPTELLRHADLALYLAKGAGKATWQRYRSDLHTAMVERLALRTSLLEAIDAQQFVLQYQPIVNVRTQAVVGVESLVRWQHPERGLLAPYHFIELAEESGAIVGLGAWVLRESLRQFAAWHASDPEMALRYVSVNVSARQFRQPGFVDQVSQALEDAGARPEWLLLEITESLVLRDAEQVWTDLKALRALGVRIAIDDFGTGYSSLSYLRQLPVDVLKIDKSFIDDILDSKQQRALVDAIVTLARNLDLTVVAEGIEDAAQRAMLERMGCPYGQGYLFSKPVWPAEIPPLLILAVVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5E6</accession>
    <name>U5W5E6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family 2 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560569.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29855"/>
    <dbReference type="EnsemblBacteria" id="AGZ44232">
        <property type="protein sequence ID" value="AGZ44232"/>
        <property type="gene ID" value="AFR_29855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6047"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_7_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="233" mass="25625" checksum="64D945BB208C558A" modified="2014-01-22" version="1">MILLFPVYQPSDRLPSLLTELRAAAPEADLLVVDDGSGPPSTQVLETSETLGATVLRHDRNRGKGEALKTGFRYAAEHRPGQTVVCADADGQHSVEDILRVAARAAETGHIVLGVRQFEDDVPLRSKFGNAVTQTLFRAATGRPVQDTQTGLRAFPADQLEWLLTVQGERFEYEMSVLIEAVRTGRPMDEVVIDTTYLKDNASSHFGALSDSARIYWPLLRSLALSLRAPSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5V5</accession>
    <name>U5W5V5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560881.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31440"/>
    <dbReference type="EnsemblBacteria" id="AGZ44548">
        <property type="protein sequence ID" value="AGZ44548"/>
        <property type="gene ID" value="AFR_31440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6363"/>
    <dbReference type="eggNOG" id="COG0738">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_727205_0_0_11"/>
    <dbReference type="OrthoDB" id="2097251at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="366" mass="35553" checksum="9840A5FC9BE91F0B" modified="2014-01-22" version="1">MIGRVQQAGIAQAVLGYAITGLGACLVLLADDLGVAPEQLGWLPATFGFGLLALALTGPLLLRGGPRPALIGGSLVVALGVTLLALAPNTPAAVTGALLLGAGGAAFVLVTPALLSGSEAAIQLTKVNALSSIASVLAPAAIGGLDATGLVGGRLALLIAVPPLLYLAATTARSRSAQPGPAPTDGPPSAHGRPAPGLVARRWAGVVLAVSVEFCFTIWAVARLAEAGLALATAAVVGTAFPIGMALGRLAGPALIRRIPVVPVGAAVTAAGAVLVAAAGHPAAVTAGLVVAGAGVATLYPVALAGLVATPRLSAAHAASLGALASGTAILAAPAALARLADVVDLRLAFLITLPLLALLLLIRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Y8</accession>
    <name>U5W5Y8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable 2-phosphosulfolactate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_30835</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58738"/>
            <dbReference type="EC" id="3.1.3.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ComB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.71" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560762.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30835"/>
    <dbReference type="EnsemblBacteria" id="AGZ44427">
        <property type="protein sequence ID" value="AGZ44427"/>
        <property type="gene ID" value="AFR_30835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6242"/>
    <dbReference type="eggNOG" id="COG2045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094942_0_0_11"/>
    <dbReference type="OrthoDB" id="1617556at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005238">
        <property type="entry name" value="ComB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04029">
        <property type="entry name" value="2-ph_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="SSF142823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="254" mass="25640" checksum="540EDEE46BE5F59F" modified="2014-01-22" version="1">MNEAWTQRDSRIRFEWGPTGAGAVAVGAAFVAVVDVLSFTTTLSVAVDLGISVLPYRWRDGSAARVAAEQDAVLAVGRREVVAPGQVSLSPATLRGVSGVERLVLPSPNGATVAAGLAEGGARLVGVSLRNAEAAARWVARQVGADDVVAVVAAGERWPDRSLRPAVEDLWGAGAFISALVGAGLRGHSIEAGAAVCAYESVAGSVPTALGDCASGRELIAHGFPEDVASASEVGSASAVPVLSDGWFVAASDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W674</accession>
    <name>U5W674_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Family 2 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_31795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560952.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31795"/>
    <dbReference type="EnsemblBacteria" id="AGZ44619">
        <property type="protein sequence ID" value="AGZ44619"/>
        <property type="gene ID" value="AFR_31795"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31795"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6436"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_4_1_11"/>
    <dbReference type="OrthoDB" id="1064289at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="16"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="329" mass="36315" checksum="7008BB6D2015AE84" modified="2014-01-22" version="1">MPPAPRHDQWSTPSVTVVIPALNEEHNLPLILENLPPVDEVIVVDGRSHDDTVAVAREVRPDVVVIRQTRTGKGNALVCGFAASTSDIIVTLNADGSADPQEIPRFVDALLSGAEVAHGSRFRPGGDHRDAGALERTGHQALSRLVNLFFGTRFTDLSCGYNAYWRALVPALDLPAPDVRGPKRGLAWGEGPEIDTLATIRMAAQGLRVVEVATVGYPRLDGDRPRRVLPAAVRAVRTAYSEYVRRWKIGRRPAPQRRLSEPLRPLHENEPTGRHAARHSVPPPPRDRDGGRRGRRRPDLTVIQGEGRDDRWDGTTHLRAVPGENFRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6N9</accession>
    <name>U5W6N9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_26595</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364421.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26595"/>
    <dbReference type="EnsemblBacteria" id="AGZ43581">
        <property type="protein sequence ID" value="AGZ43581"/>
        <property type="gene ID" value="AFR_26595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5390"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="26157" checksum="A3D65992F6604124" modified="2014-01-22" version="1">MTAAAHLDSLYRTYDDIGTAVTALDGVTATFPAGTFTAVMGPSGSGKSTLLQCAAGLDRPTSGRVFIDGAELTATKDTAMTKFRRRRVGFVFQDYNLLPALTVEQNVLLPLRLDGRRADRARSTEILTRLGLGERLHDLPEQLSGGQRQRVAIARALVTEPAVIFADEPTGALDLRSAREVLTLLRAVVHEHGRTVVMVTHDPVAAAYADSVLFLADGRLAGSLSAPTAEAVAERLAHLGDRVGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W711</accession>
    <name>U5W711_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561325.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33650"/>
    <dbReference type="EnsemblBacteria" id="AGZ44988">
        <property type="protein sequence ID" value="AGZ44988"/>
        <property type="gene ID" value="AFR_33650"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33650"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6810"/>
    <dbReference type="eggNOG" id="COG3285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008325_1_1_11"/>
    <dbReference type="OrthoDB" id="2061599at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014145">
        <property type="entry name" value="LigD_pol_dom"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02778">
        <property type="entry name" value="ligD_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="318" mass="34609" checksum="6513F4E66177A586" modified="2014-01-22" version="1">MAEKEERDGVPLTNLDQELFPGAGATKRELVDYLDAVRERMIPVLRDRPLSVVRVLRGQDKFMQKNLPKYTPEWVPRTAVWAEASHREVQYALCNDRRTLLWFANQRAVEYHPTLMTVGDRDHPTHLIMDLDPPEGAGFDVVVKAAGLVRQALADAGMAGAVKTSGSKGVHIFVPLDGSAATIDVAAATRAVAVRAERLDPALATTAFIREDRHGKVFLDATRAGGATVVAAYSPRVRPGVPVSFPVAWDDLGDVTPGDFTVRTAPGLLKDGDPWAQAMPDPQPLDPGLVEEGHTIPVARVAAMHEGKRRARAKRAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W775</accession>
    <name>U5W775_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F420-dependent protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33680"/>
    <dbReference type="EnsemblBacteria" id="AGZ44994">
        <property type="protein sequence ID" value="AGZ44994"/>
        <property type="gene ID" value="AFR_33680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6817"/>
    <dbReference type="eggNOG" id="COG3871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123922_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019920">
        <property type="entry name" value="F420-binding_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03618">
        <property type="entry name" value="Rv1155_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="16224" checksum="13E10CCB39EB1E2B" modified="2014-01-22" version="1">MDLMSDDTKLWSAIAATDLCTLATIKRDGRPQLSDVNYTADTPTRVLRISTRETLAKVHNLRRDPRGSIRVIGPGGGGYVAAEVRASFSPPSADEHDATVEELVDIYRSIRGEDHPDWDDYRRAMVADGRIVLTLHVERLYGWVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-02-10" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7C2</accession>
    <name>U5W7C2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cu2_monoox_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34050"/>
    <dbReference type="EnsemblBacteria" id="AGZ45068">
        <property type="protein sequence ID" value="AGZ45068"/>
        <property type="gene ID" value="AFR_34050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6890"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039620_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016715">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014784">
        <property type="entry name" value="Cu2_ascorb_mOase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036939">
        <property type="entry name" value="Cu2_ascorb_mOase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024548">
        <property type="entry name" value="Cu2_monoox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008977">
        <property type="entry name" value="PHM/PNGase_F_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03712">
        <property type="entry name" value="Cu2_monoox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49742">
        <property type="entry name" value="SSF49742"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cu2_monoox_C" evidence="3">
        <location>
            <begin position="283"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="388" mass="40723" checksum="A3FBE6E9B6F36FD0" modified="2014-01-22" version="1">MTLVTAFAAVAACGSDADNSVAPPPAGSHSAHAGASSAPPQPLRAGERFLDLKMAEAYTPTPPEGGGSDEYRCQMIDPGLTRAAFLTGTQFAPENVAIAHHAIVYAVPPGGAAAVRKQDAKTPGLGWQCFGGTGVAGAEVEEGDAAWVDTWAPGATETLLDQDAGYKLEPGGLIVLQIHYNLLATDGKPTGSDLSAVRLRLTDGTPCTRELETWSLDGPIDLPCAADESGPLCDRAASIADVTKRFGPEVGEMADDQVEECNRGGAPKPGDTQTCDHKVEAPATLFAGFGHMHMLGRALKVELNPGTPKAKVVLDVPQFDFDNQRLMKLPSPVEIGPGDTLRVTCKHDVGLRKLVPQLKKLPPRYVVWGDGTSDEMCIGIMAVSPRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7E0</accession>
    <name>U5W7E0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycoside hydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_34175</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561430.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34175"/>
    <dbReference type="EnsemblBacteria" id="AGZ45093">
        <property type="protein sequence ID" value="AGZ45093"/>
        <property type="gene ID" value="AFR_34175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6915"/>
    <dbReference type="eggNOG" id="COG1501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_301041_0_0_11"/>
    <dbReference type="OrthoDB" id="469334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033403">
        <property type="entry name" value="DUF5110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03422">
        <property type="entry name" value="CBM_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17137">
        <property type="entry name" value="DUF5110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666149" evidence="4">
        <location>
            <begin position="29"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="5">
        <location>
            <begin position="869"/>
            <end position="993"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="993" mass="107517" checksum="F82AA7573EAE85D2" modified="2014-01-22" version="1">MQPSRRSFLKVAGLAVGVPALPLGRALADETLTDYGSHVVDSRSLTVTSSTGQQLRITAYGGHVVRVRAVRNGEVFFADNRYEMVDPASHSGMGGTLQVTESGDAFTITTAPADGVRVVLRKSPLRVEFSSRNDNAALVREDTTHSVSWGGTVVRQTFTPPAAGERFVKAGHGLYGRAPKVDRTGDIVSHNYGLQSSHVDQAPAIVPMYLSSRGYAVFFNTTFDTTFNFGNGGTYEFSADGHGASGARPQLDYFLIKGPEFPKLLDRYTQLTGRPRFPRLGMFGLQLSDKNFPAVSDQSWWTSRITALRDAGFPLDVQVHDNRWRAGTGAWGGSWFEFSPERWPDPAAFKRWADEHGILTTLDYNRNNSNEMAGWVGGPPPGYSFRPADLAGVADNDAVPDWSNPATRAWAWNVFWTKAFNPALGFPGDALWIDEPDEMGPIPYDALAANGQRWSELRNAFFLYCQKAAGQEGWDPATAGHIGHTRRPWIFTRGATAGQQRYGHLWTGDINSDYTEMQEQIRGMQNAGLGGFPFANIDAGGFFGAVISDGLYRNWVAAWASTSPIWRPHSNGDTGAQGLAASRWPIDQGTASRTEFLRYARVRYTLLPYLYTIAHTAHATGVPMARAMVIDHQHNPMAYTHDLQYLWGPSIIVMPVTTDTDGAVQRVWLPAGDTWYDFWSDAQNVGSDSTEKRYVTSTGEIIMYVRAGSILPKYRYAQSTAFLDKSQLELEVYTGQDGSFTLYEDDGVTEEFRISNAAATTGLTYTHAALRVVVAHPAGTYRGAPAARRYVVRLHGLTAPIGMRVGAGANLPAFTGESAAILNGSGQVWNAARRILTVVTPMINVSAGGGVAATVEPSGAAFPAPSRQVVYEAEDATRSGATLASQHNGYTGNGYADFTNATGDHVEWTVPVAAGGVQPLGFRYANGGTTDRPLRISVNDTIVAAHLSCPPTGSWKTWRTATVTVSLPAGTAVRIRATTTGASGANLDSLSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7G4</accession>
    <name>U5W7G4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28395</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560277.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28395"/>
    <dbReference type="EnsemblBacteria" id="AGZ43940">
        <property type="protein sequence ID" value="AGZ43940"/>
        <property type="gene ID" value="AFR_28395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5757"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_18_2_11"/>
    <dbReference type="OrthoDB" id="1745184at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="197" mass="21617" checksum="7E3AE6737CBFF47F" modified="2014-01-22" version="1">MTAPKQDRSRATRRRLLETAIRCLATHGWEASTVGLIATEAGLSRGAVQHHFPTREALIIAALEHMFADRAALLDALPDPSGTDPDRIHTVVAGLVEAIGGELFRAALQVWTVAAADPDLRSAVVPLERHFAREVHRRAVRLLRVDDTDPHMRGLIQATLDLARGLALADILTDDSRRRAHVVRTWSTHLALALPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7J3</accession>
    <name>U5W7J3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MFS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_33620</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33620"/>
    <dbReference type="EnsemblBacteria" id="AGZ44982">
        <property type="protein sequence ID" value="AGZ44982"/>
        <property type="gene ID" value="AFR_33620"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33620"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6804"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_13_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="167"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ44982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="410" mass="42178" checksum="74E880CEC54FD30F" modified="2014-01-22" version="1">MLRDTDFRKLWAGMTVSKLGTSVASVTTPLIAVQVLDADAFSVSLLTAAAWLPWLLIGLPAGAWIDRVTRKPVMILSDLAAAALVISVPVAAGLGHLTMAHLLAVALLLGVATVFFTVAWTAYLPAMFDKDDLVGANSALQSTESASQVAGPAVGGLLITAVSAVAGLVVDAFTFLISAFSLWRIRRPERRPAATERQSLRSDIAQGARWLINDRFLRNLTLYGASSNLWLTGINALTVVFLVREVGVSTTGVGLLMAAIALGGVAAATVTPRLVSGLGGARSLVACKVFGGVASLLVPLTQPGPGLTMFVLGMLGIAAGAIAGNVVSASFRQAYVPPGLLGRVLTSMQFVNFGAIPIGAVLGGAAAGLAGTRGAMWIMAIGYALSGLILVLGPFRGRRDLPVSEVVLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7L0</accession>
    <name>U5W7L0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-1,2-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33695</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561334.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33695"/>
    <dbReference type="EnsemblBacteria" id="AGZ44997">
        <property type="protein sequence ID" value="AGZ44997"/>
        <property type="gene ID" value="AFR_33695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6820"/>
    <dbReference type="eggNOG" id="COG1470">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003690_2_0_11"/>
    <dbReference type="OrthoDB" id="100818at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005887">
        <property type="entry name" value="Alpha_mannosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041371">
        <property type="entry name" value="GH92_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012939">
        <property type="entry name" value="Glyco_hydro_92"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07971">
        <property type="entry name" value="Glyco_hydro_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17678">
        <property type="entry name" value="Glyco_hydro_92N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01180">
        <property type="entry name" value="aman2_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666167" evidence="1">
        <location>
            <begin position="26"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_92N" evidence="3">
        <location>
            <begin position="33"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_92" evidence="2">
        <location>
            <begin position="265"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1080" mass="114617" checksum="6E0A04BF495747EE" modified="2014-01-22" version="1">MTRRFLVAVLLAASALYASPAQARAAEDDPVDLVHPFVGTQNFGNTFPGASAPFGMVQVSPDTGGQGGYDYQQSSIYGFSQTHLSGVGCGVAGELPMLPTTGAVTTSDPNAYRSPYSHDEEDAEPGYYRVGLSKYQTKAELTATPRTGWQRYTFPETGAANVLFNTGKANQSVFDSEIHVVDDRTVEGRVHAGNFCAGKDDHTVYFTASFDRPFSTTGTWRGSTLTPGGKDASGTGGNGAWVTFDATQDRDVVVKVALSYTGPEGARKNLTAETADSYDFDATRAKLRATWAGELDRIKIDGGAADRRSAFYTALYHSLLHPNLAGDVDGRYVGFDREVHTAQGYTPYQNFSLWDTYRPQNQLLEMLAPDVARNVALSVVAIGRDGGWLPRWALANSETNIMTGDPVTPFLVEAWSKGLLSGYEEEAYALLRKNATAQPPADSPYNGRTGQDYYADRGYIPSGLELGKDCVDKGGDNDCDHPASATLEYAAADASLALMAEGLGKEADAKLFAGRGQWYRNLWDSSIQQFRPRTVDGTWLTPYDPIAAGEQFHEGGAHQYQWLVPQDPAGLVQLMGGRKATEKRLDDFFVYDKLLTDPAGTARTDWIASPYDYYAKSTYNPNNEPDLLAPYMYHWAGDPAKTATVVRAAMTLFTTGPDGMTGNDDLGTMSAWYVFSSLGLYPTMSGANFLAVSSPQFPRATVKVGTKRLVIDAPGASDANRYIQRVRLNGVPVAKSWLDWSAVARGGTLSHTLGTSPSGWGTSLLAQPPSVNNKAPADNRTHVDASVRPASAALAAGSATAQKAAFEVDVVGQAPLAIAPRVTAQAPAGWKVTVAPRVPTLLLTRHLPVGTTAKVTVEAPAGTPAGTYQVDVSVSATGTETVKRTVTITVREPVTCASTAGTACLVDLGTERTTDGTATVAATTEGNFDGGGWSYDADLLPAAGPVTWGDVPYQAPDPTGTAKNFVQSRGQSLLLPPGNHPAVNLVATTHNGPVTAALTIGYTDGTSAAATVTVADWCGTASAGTTAVLSMSHRIKAGQGVDGPPVSLFGLDLPVTQGKQIRSITLPNDPRLYLYAVTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Q1</accession>
    <name>U5W7Q1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative hydro-lyase AFR_34835</fullName>
            <ecNumber evidence="3">4.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34835</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-glutamate cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34835"/>
    <dbReference type="EnsemblBacteria" id="AGZ45223">
        <property type="protein sequence ID" value="AGZ45223"/>
        <property type="gene ID" value="AFR_34835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7048"/>
    <dbReference type="eggNOG" id="COG4336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059759_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01830">
        <property type="entry name" value="Hydro_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009906">
        <property type="entry name" value="D-Glu_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038021">
        <property type="entry name" value="Putative_hydro-lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016938">
        <property type="entry name" value="UPF0317"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07286">
        <property type="entry name" value="DUF1445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029755">
        <property type="entry name" value="UCP029755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160920">
        <property type="entry name" value="SSF160920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="24880" checksum="285134DD69E7140F" modified="2014-01-22" version="1">MTQANLIAVPRDWAWDMLLYAQRNPKPCPVLDVSDPGSPVSVLAPGADLRTDLPLYRIWRDGKLVDEVPDATAAWRDDLVAFLIGCSFSFEESLIAAGIDVRHISTGRNVPMYRTNRPCRPAGRLHGELVVSMRPIPADRVADAVTISGRFPAVHGAPVHIGDPAGLGLTDLDSPDFGDPVEIRPGEVPVFWACGVTPQAAVMASQVPFAITHAPGHMFLTDVPDTEYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7Q8</accession>
    <name>U5W7Q8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">zf-HC2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_34885</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561569.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34885"/>
    <dbReference type="EnsemblBacteria" id="AGZ45233">
        <property type="protein sequence ID" value="AGZ45233"/>
        <property type="gene ID" value="AFR_34885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7059"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056526_0_0_11"/>
    <dbReference type="OrthoDB" id="1740922at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041916">
        <property type="entry name" value="Anti_sigma_zinc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027383">
        <property type="entry name" value="Znf_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13490">
        <property type="entry name" value="zf-HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="zf-HC2" evidence="3">
        <location>
            <begin position="10"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="262" mass="27870" checksum="F7566B26E02509E8" modified="2014-01-22" version="1">MTQEQHFDVAAYALGVLDDRDATRFEDHLIDCPTCAIELESLLPVVDILSDVDADALVATEQSRRDGLVLKKMIGEVKRERRRANSRRLYSLAAAVVVFAMLSIGALFAGGQWLGPDQQNNPSTPAQARQGSSKQLDPLPLSEGGVGIGGPDLVGERLGGTDPRSGVRADVALEAKDWGTQVSFAVSSIKGPLTCRLVAVRTDGSTEVLSTWQVGDKGWGTAAQPDPLLLQAATALPRDEIAHLQIQSVGPKGATETLVRVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7X2</accession>
    <name>U5W7X2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_35285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561649.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35285"/>
    <dbReference type="EnsemblBacteria" id="AGZ45313">
        <property type="protein sequence ID" value="AGZ45313"/>
        <property type="gene ID" value="AFR_35285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7139"/>
    <dbReference type="eggNOG" id="COG1200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144847_1_0_11"/>
    <dbReference type="OrthoDB" id="1944256at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016499">
        <property type="entry name" value="NucleicA-bd_Rv2694c_prd"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006910">
        <property type="entry name" value="NA_bind_Rv2694c_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="14046" checksum="1FDCA1D217932BFA" modified="2014-01-22" version="1">MSTDERTGLRRFFERLTASEEELDAQELQRDSAKCGAMPAGQCHRGEVVSVSGRLRTVAYTPRTNLPTLEADLYDGSDVVTLVFLGRRSIAGIEPGRQLTARGRIALRDDRKVIYNPYYELEAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8C2</accession>
    <name>U5W8C2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fe(2+)/(Mn2+) transporter pcl1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_36010</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36010"/>
    <dbReference type="EnsemblBacteria" id="AGZ45458">
        <property type="protein sequence ID" value="AGZ45458"/>
        <property type="gene ID" value="AFR_36010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7289"/>
    <dbReference type="eggNOG" id="COG1814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038957_3_0_11"/>
    <dbReference type="OrthoDB" id="1717935at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:cellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="PTHR31851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="23562" checksum="B6A952328F2A51F1" modified="2014-01-22" version="1">MTSPEHHADVSGGWLRAATFGAMDGLVTNIALIAGVGGGGVGRHTLILTGVAGLVAGAISMGLGEWTSVRTQNDQIAAELGKELHELRVNPDGEADELVGLWTARGLPEPLARQVADVLKMHPEQALRVHAQEELGVVPDELPSPWIAASSSFVCFALGAVVPLLTYLLGSDSLLAALMVGGVGLFAAGALVARFTGRAWWRSGLFQLLLGAAAAGATYVVGALIGVGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8C9</accession>
    <name>U5W8C9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_35715</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561735.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35715"/>
    <dbReference type="EnsemblBacteria" id="AGZ45399">
        <property type="protein sequence ID" value="AGZ45399"/>
        <property type="gene ID" value="AFR_35715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7229"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_0_11"/>
    <dbReference type="OrthoDB" id="1155802at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="221"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="286"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ45399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="443" mass="47210" checksum="D037DA81DA65C0D4" modified="2014-01-22" version="1">MLILVGLLLVILVTAVTGYFVAQEFGYVAVDRSRLRQEAEGGDKAAQRALEVTERLSFVLSGAQLGITVTALIVGYVAEPFLGEGLADLLGLTGLPSGVSMPISIVLALLIATVVQMVLGELAPKNFAIARSETLAKALSRSTLMYLTVFGPIIKLFDKAAGGLLRRIGIEPIEELPEGATEEDLEQIIAESRAEGHLGPELSLLLDRGLDFRRRTASEVMIPRVDVVVIQATDPASQVIELLDTGRSRFPVRAGNGVDEIVGVVGIADVLPVPPADRHDVQVETLMAPALFVPASLRLPAVLDRLRGDHRQLACVVDEYGGFAGIITLEDIAEELVGPISDEDDLPEPSPARQEDGSWIVPARWRIDEVTDATGADLPEHPEYDTVSGLVMSELGRVPQVGDSVSLPSGAVLEVLSVDRHVPDSVRLTMPVAAGSALEEGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8F0</accession>
    <name>U5W8F0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_35815</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyltransferase 87 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561755.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35815"/>
    <dbReference type="EnsemblBacteria" id="AGZ45419">
        <property type="protein sequence ID" value="AGZ45419"/>
        <property type="gene ID" value="AFR_35815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7249"/>
    <dbReference type="eggNOG" id="COG5542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043514_0_0_11"/>
    <dbReference type="OrthoDB" id="1441955at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018584">
        <property type="entry name" value="GT87"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09594">
        <property type="entry name" value="GT87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="321"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="390"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="394"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ45419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="471" mass="52656" checksum="90AF283B0742537D" modified="2014-01-22" version="1">MRRARTELLLLGALALLAVVVRWVSRDEITADMRIFLQWYRWLDEAGGFPGLDREIGNYNAPFLYLLATLTYLPGPAILKIKAAWTVFDVLLVYFGYRIVALRYTGWRVPALAASVLAFLPTVVVNSSFWGQCDAMWASFCLGGLYFLLRGRQWHAVALLTVAIAVKPQAVFIFPTLVLLLLAGRQRWRTLLAAPAIYITLDLPAMLLGRDPIELLTLYSPARQTLHVPDLTSKAPTLYAFLPVTTRLDTLRTLGYVFAAVLVLGVIYTLIASRAKLDADRLVTAAALFSILVPFVLPGMHDRYFFLADALTVILTFYRPRLWLVPLLVQVGSLLSYPRFLFQGAEHGSFVPLPVLAALMLTALLITAHALVQDLRPGLSHATGLNQAAEPLVRRRGAADHDPGSSAWERRSSMKREAAGDPIRDRRDREYADQRPPRPNRRTTMPRPLSADKAQLQASRAIRSARRPRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8I2</accession>
    <name>U5W8I2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome maturation factor RimP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rimP</name>
        <name evidence="7" type="ORF">AFR_35990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for maturation of 30S ribosomal subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RimP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561790.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35990"/>
    <dbReference type="EnsemblBacteria" id="AGZ45454">
        <property type="protein sequence ID" value="AGZ45454"/>
        <property type="gene ID" value="AFR_35990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7285"/>
    <dbReference type="eggNOG" id="COG0779">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070525_3_0_11"/>
    <dbReference type="OrthoDB" id="1595498at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01734">
        <property type="entry name" value="YlxS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01077">
        <property type="entry name" value="RimP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003728">
        <property type="entry name" value="Ribosome_maturation_RimP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028998">
        <property type="entry name" value="RimP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028989">
        <property type="entry name" value="RimP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035956">
        <property type="entry name" value="RimP_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33867">
        <property type="entry name" value="PTHR33867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02576">
        <property type="entry name" value="DUF150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17384">
        <property type="entry name" value="DUF150_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75420">
        <property type="entry name" value="SSF75420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="DUF150" evidence="5">
        <location>
            <begin position="48"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="DUF150_C" evidence="6">
        <location>
            <begin position="127"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="192"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="222" mass="23636" checksum="F0F0EFF30BCDC378" modified="2014-01-22" version="1">MTQRGRGGARPANRQGGGRSRDAVPETQAPAAPRIDLVAARSRVRAVIEPVVVAEGFDLEDVTLSRAGRRHVVRVLVDADGGISLDDVAVVSRAISDALDTADESQGEVLAGEYQLEVGSPGVDRPLTLPRHWRRNVSRLVTVTVAGRSLTGRVTAADETGIVLDVDGKTSEIPYAGLGPGKVQIEFKRLDEAVFPDESEDDAGDDEDEDEADDEDGEGEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8K6</accession>
    <name>U5W8K6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560652.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30275"/>
    <dbReference type="EnsemblBacteria" id="AGZ44315">
        <property type="protein sequence ID" value="AGZ44315"/>
        <property type="gene ID" value="AFR_30275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6130"/>
    <dbReference type="eggNOG" id="COG0785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082333_0_0_11"/>
    <dbReference type="OrthoDB" id="1480014at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021315">
        <property type="entry name" value="Gap/Sap"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11139">
        <property type="entry name" value="SfLAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="234" mass="24999" checksum="3438D0241352A459" modified="2014-01-22" version="1">MDWTLLAALAGLALVDSTSVGTLLIPLWMLLAPRLRVRPFFLYLATVAAFYFVVGIVLTAGAGTLRGLIGRSDTLSWVQLVVGVALFALSFWFEPKAVRRRRARRGGPDPADRWRARLSTAEASPGAMVGLGVAAAGIEVMSMLPYLAAVGLITASGAGATVWIPVLAGYVLVMILPALLLLGIRQALGTRIETPLRVISAWLSRHLDGALSWIFAIIGFLLARDAVIRLDLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8M4</accession>
    <name>U5W8M4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein piccolo</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561830.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36190"/>
    <dbReference type="EnsemblBacteria" id="AGZ45494">
        <property type="protein sequence ID" value="AGZ45494"/>
        <property type="gene ID" value="AFR_36190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7323"/>
    <dbReference type="eggNOG" id="COG3599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_723234_0_0_11"/>
    <dbReference type="OrthoDB" id="1500223at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019933">
        <property type="entry name" value="DivIVA_domain"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03544">
        <property type="entry name" value="DivI1A_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="354" mass="39613" checksum="BB530D0B2543D232" modified="2014-01-22" version="1">MTSQGQRFRRRALRRGYKVDEVDAFLDRVEATLAGEQSGAPVGAQEVHDVVFRVRFGGYDEWQVDLHLDRVERQLAEFEDRGGRAPDARMTPDRMQPEPMRGGMQSDRMSQAMGRSAPPTERLQAPVRDDRQFAREDQFGREDQYAREEQFAPRDDPYGREDRMQPQQLPQRPQMPAPDPYGRYEEPGYGQQAPQRPQAPQGYDMGGFDGFEPGRHGKTDMTAEIRMPERDRGGYGGGGPSPMSAPPMSGPPMGGPGMGGPPMGGGAQGGSPEVYRVDQLRRTFQPRRFGSGYDPVQVDRLFESILQAMTGRGPMPVPENELDTLQFGLVPGGYFEAEVDAALREVKDILLHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W924</accession>
    <name>U5W924_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31175</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560829.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31175"/>
    <dbReference type="EnsemblBacteria" id="AGZ44495">
        <property type="protein sequence ID" value="AGZ44495"/>
        <property type="gene ID" value="AFR_31175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6310"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_8_11"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="128"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="128"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="229" mass="25187" checksum="7975471DE5C1EA7C" modified="2014-01-22" version="1">MATVLLVEDDHVVRGAMLRSLADRGHAVHAVGTALDALRRVAAETPDLVVLDLGLPDLDGSDALRMLRGITDVPIIIATARDDEQTVVRLLRAGADDYMVKPFTGAHLDARITTVLRRVGRASRTAQPAVHEVGELRVDIGERSALLAGEALALTRKEFDLLAYLAARPGRVVSRRELLEEVWRQPSVGEDQTIDVHLYWLRRKLGESAAKPRYLRTVRGVGFRLVAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W962</accession>
    <name>U5W962_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide chain release factor 1</fullName>
            <shortName evidence="5">RF-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">prfA</name>
        <name evidence="7" type="ORF">AFR_37420</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Methylated by PrmC. Methylation increases the termination efficiency of RF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562073.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37420"/>
    <dbReference type="EnsemblBacteria" id="AGZ45738">
        <property type="protein sequence ID" value="AGZ45738"/>
        <property type="gene ID" value="AFR_37420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7569"/>
    <dbReference type="eggNOG" id="COG0216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_0_1_11"/>
    <dbReference type="OrthoDB" id="928964at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0488">Methylation</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="6">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="5">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="363" mass="39481" checksum="C8A17BD078E50381" modified="2014-01-22" version="1">MSTDRLSMLLAEYADLEKQMEDPSIHSDQALVRRVGRRFAELAPLYTANVELEAARADLAAAKELAAEDPSFAGEVEAVAAALQPLEEKLGEMLIPRDPHDAKDVIVEIKAGEGGQESALFAGDLLRMYTRYAEKHGWIVEIIDSQDSDLGGVKDVSVAVKTKGVPEGGHGVWSRLKWEGGVHRVQRVPVTESQGRIHTSAAGVLVLPEAEDVDVHIEPGDIRVDVFRSSGPGGQSVNTTDSAVRITHLPTGTVVSCQNEKSQLQNRESAMRILRSRLLALAQEQADAAASDARKLQVRTVDRSERIRTYNFPQNRISDHRIGYTAYNLDLVLGGELDAVLDALGEADRAARLAGGTEISRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9D2</accession>
    <name>U5W9D2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_36830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561958.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36830"/>
    <dbReference type="EnsemblBacteria" id="AGZ45622">
        <property type="protein sequence ID" value="AGZ45622"/>
        <property type="gene ID" value="AFR_36830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7451"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076523_0_0_11"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="6">
        <location>
            <begin position="22"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="26775" checksum="28A48F33F4BC8362" modified="2014-01-22" version="1">MTRSWLRSYLLLLRWSVIRLRYLLPMVLVVQVFLAVGIVVGFAYLLPSIDPATALYLSTGAPTLGLITIGMVMAPQLVAQSKTEGTFTYNRTLPVPRTAVLAADVTTWLLPGAPGLVLGLLAAVLRFDLNLHVSWWVLPAMLLVALTATTIGFAMAYALSPSLTSVISQAIVFVALMFSPINFPADRLPEWLRAVHEVLPFQYMAQAVRETLTSPPGGITVLPFAVLAVWCAAGLAITLRVMTRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9P8</accession>
    <name>U5W9P8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38075"/>
    <dbReference type="EnsemblBacteria" id="AGZ45869">
        <property type="protein sequence ID" value="AGZ45869"/>
        <property type="gene ID" value="AFR_38075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38075"/>
    <dbReference type="eggNOG" id="COG3677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190603_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="52" mass="5758" checksum="F88864E7B3480080" modified="2014-01-22" version="1">MSDARSAPLYCPYCGEEDLRPNEASHGAWECHSCARVFTVKFNGLLAKGVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9S2</accession>
    <name>U5W9S2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561057.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32335"/>
    <dbReference type="EnsemblBacteria" id="AGZ44725">
        <property type="protein sequence ID" value="AGZ44725"/>
        <property type="gene ID" value="AFR_32335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6544"/>
    <dbReference type="eggNOG" id="ENOG502Z9FH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064457_0_0_11"/>
    <dbReference type="OrthoDB" id="908514at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="322" mass="34515" checksum="39C4C108DB91E544" modified="2014-01-22" version="1">MGSGHWSTNVYSAAANYRAATGKSAFAYSDSGARKAHAALDPMNVGKRESRDSDEHPQSTPIAVLFDVTGSMGSVPRVLQTKLPQLLGLLTRKGYATDPHIMFGAIGDATCDKVPLQIGQFESDNRMDEDLARIVLEGGGGGQRRESYELAMYFMARHVSLDSMEKRGRRGYLFIIGDEMPYTQVKPKEVKTFVGDRLNEPITVEAMIAELRRSFEIYYIMPTAAGWGGDAEILGYWRQLLGQHVIALDDLDAVCETIALTVGLAEESIDLDAGLADLAEFGSAAGDTVGKALAPIGAGRGAVVRSPLDLGGAATPGGNERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAJ2</accession>
    <name>U5WAJ2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562473.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39425"/>
    <dbReference type="EnsemblBacteria" id="AGZ46139">
        <property type="protein sequence ID" value="AGZ46139"/>
        <property type="gene ID" value="AFR_39425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7976"/>
    <dbReference type="eggNOG" id="COG2105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048475_4_0_11"/>
    <dbReference type="OrthoDB" id="1984556at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003839">
        <property type="term" value="F:gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017939">
        <property type="entry name" value="G-Glutamylcylcotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935">
        <property type="entry name" value="PTHR12935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="SSF110857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16717" checksum="36AF81237893C85C" modified="2014-01-22" version="1">MGHYAAYGSNLDPARMRAYCPHSPMVGVGWVEGWRLTFAGEGDMGWEGAVTTIVESPGDRVFVSLYDVHPWDAAQLDEVEGVTAGAYQKLTVRVSTLEGEFSAWVYVFDGYEGGMPTAWYLSEIANAAEKAGAPDDYVADLRQRPTRTSSPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAQ7</accession>
    <name>U5WAQ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45050">
        <property type="protein sequence ID" value="AGZ45050"/>
        <property type="gene ID" value="AFR_33960"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33960"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6874"/>
    <dbReference type="HOGENOM" id="CLU_1727437_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665728" evidence="1">
        <location>
            <begin position="21"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16343" checksum="68CA9B59AD1CCF40" modified="2014-01-22" version="1">MLRRAAPRLIVVVGLNALSAFSSAVTPELVAYTIQSLAFVLVFGALGSREWWRYVRHGNPAPWQVAHGGATAVFVTDPGRDRFQVVAQLREHGGLDFGQALQRAEDPSRPVWDDLTSESADRLGAVLVDAGASVVVKPRPSPSWARDVTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB41</accession>
    <name>U5WB41_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562765.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ46433">
        <property type="protein sequence ID" value="AGZ46433"/>
        <property type="gene ID" value="AFR_40895"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40895"/>
    <dbReference type="eggNOG" id="ENOG5032SNF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119015_0_0_11"/>
    <dbReference type="OrthoDB" id="1647498at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17329" checksum="CBFA7AD857AC58D9" modified="2014-01-22" version="1">MSWAAFRTALTAVNGTTLAGLGLAVLSGARLRRAPGGILIAENYRHRMPPATCFTVGSVIITRRTADWLLHESRSELLAHESRHAGQYAVLGPLFWPAYWASSGYSWAMTGTYGTRNAFERHAGLERGGYRRAPLRPGLQRFTRSAATGSTPPSAGSPADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBC7</accession>
    <name>U5WBC7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_40190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562624.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40190"/>
    <dbReference type="EnsemblBacteria" id="AGZ46292">
        <property type="protein sequence ID" value="AGZ46292"/>
        <property type="gene ID" value="AFR_40190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8134"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_19_2_11"/>
    <dbReference type="OrthoDB" id="1484726at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="23381" checksum="AD83907311087577" modified="2014-01-22" version="1">MARTHLVWDWNGTLLDDLSLVVSSTNHTFSVVGGRSVDSDEHRRRFRRPVAEFYAEILERAVDAEEFEELDRIFHEAYRVGLTTIKLAADAEAAIKAWPGSQSLLSMWFHDELVPAVQTYGLTGMFTRIDGRPTEVDGGLKAGHLARHLAEQQVAGDQVVLIGDSLDDAEAAESVGGVCVLYTGGFTDPSRLRASGRPVADTLLDAVRMARTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBU2</accession>
    <name>U5WBU2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561806.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36070"/>
    <dbReference type="EnsemblBacteria" id="AGZ45470">
        <property type="protein sequence ID" value="AGZ45470"/>
        <property type="gene ID" value="AFR_36070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7301"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_5_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="29660" checksum="8CB3416003BFD2CE" modified="2014-01-22" version="1">MVVTGGSSGIGLAAAVALARAGDEVVLLGRDPGRLQHAVDAVREAGGRTPAAYRADFAVLDEVREAGAKLVADHERIAVLVNNAGQLAPARRLTADGLDPTMQVNHLAGFLLTHLLFERLLAGATTTAPARIITTSSAAEAWGTLDVARPGRRQVSRWLAYGASKQANVLFTVEAARRWTQYGVVPTCFFPGLIRSRFGRRSPMFSLAKLAPGLFRSAEQGAETLLWLADADEALVPGGYFAWKRPFGATGRSTNADRARRLWQSSLTAVGLEASTTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBW0</accession>
    <name>U5WBW0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562814.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41140"/>
    <dbReference type="EnsemblBacteria" id="AGZ46482">
        <property type="protein sequence ID" value="AGZ46482"/>
        <property type="gene ID" value="AFR_41140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8327"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_594226_0_0_11"/>
    <dbReference type="OrthoDB" id="467214at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="438" mass="45923" checksum="AA3CB95AF182D00E" modified="2014-01-22" version="1">MASGGSAKGYALVGVVVVIVLATTGVWNPFPQIWSWINQSDPIAEGVATWQTAIGGSPKSVTISGGAIVVEYRTSVEAYGITAGVKLWKSDADWAGVAGGDSDAVVVVGRLLTKGYEVLDPATGAVRRTDTEATAVWTYADAVLDLRCPKAGECELSAWEPRGSRPMWTIATPGIGFVLDASNPDLPDTQPLTSSQFDGRAAGPRSLPNLIGLPGDGKIQVVDTAQGRVVQTRTPARDQRVTVVGSRVLTVTGVARDGTCYYSVIAHDPPSGHAVWQRDGLNLRTADNGSTCKQERDPAGGYDVVLGVDPVGRQELIAAHDGRVLWHGEKGQTVLAVNDAYALVRTSDQRTLTARSFGRGKVNWRRGINPGAQAALTPYAAVVAETKPAKVVALSPRTGGVLAEARTDAKVFAVGPSGMILVSGRDMAYVPFAGSPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBZ1</accession>
    <name>U5WBZ1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative initiation factor eIF-2B alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562970.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41925"/>
    <dbReference type="EnsemblBacteria" id="AGZ46639">
        <property type="protein sequence ID" value="AGZ46639"/>
        <property type="gene ID" value="AFR_41925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8483"/>
    <dbReference type="eggNOG" id="COG0182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_1_2_11"/>
    <dbReference type="OrthoDB" id="1290468at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="320" mass="33034" checksum="7FC1F1B391251EAF" modified="2014-01-22" version="1">MRTIDWADGAIEIIDQTALPGELRILKLTEVADLVAAIRSLAVRGAPALGVAGAFGVALAARQFTGDERGLADAVHAVEHARPTAVNLARGARRVAALIPAGPEAVLAEACAVRDEEIAASAAMAILGADLMVELCGERPRLLTHCNTGGLAAVTGGTALGVVTELHRRGALAGVIASETRPLLQGARLTAWELGEAGIDFRVAVDSAGPFLLARGEADAVILGADRICANGDVINKIGSYAHALGARRAGLPFVVVAPESTVDLETPDGDAVEIEDRGAAEITGYSHAVNPAFDVTPHDLVTAIVTDQRVIRLDRGEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCD7</accession>
    <name>U5WCD7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein fixA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_37030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561996.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37030"/>
    <dbReference type="EnsemblBacteria" id="AGZ45660">
        <property type="protein sequence ID" value="AGZ45660"/>
        <property type="gene ID" value="AFR_37030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7492"/>
    <dbReference type="eggNOG" id="COG2086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_2_0_11"/>
    <dbReference type="OrthoDB" id="1027643at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="PTHR21294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETF" evidence="4">
        <location>
            <begin position="23"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="27118" checksum="425F2FAF0C7FC0FD" modified="2014-01-22" version="1">MNIVVLVKQVPDSGAERTLSEGDNTVERTSANNVINEMDEYAIEEALKIKEAHGGEVTVLTMGPERATESIRKALSMGPDKAVHVQDDALHGSCAVATSKVLAAALGTLNADLVLCGAESTDGRVQVMPHMLAERLGIAALTGARKLTVDGSQLTAERQTDEGYEVVTAATPAVVSVWDTINEPRYPSFKGIMAAKKKPVQSLSLADLGISADEVGTAGATSAVVEFAKRPPRSAGTKITDEGTGGEQLVAYLAAEKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCG4</accession>
    <name>U5WCG4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562025.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37180"/>
    <dbReference type="EnsemblBacteria" id="AGZ45690">
        <property type="protein sequence ID" value="AGZ45690"/>
        <property type="gene ID" value="AFR_37180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7522"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077680_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665931" evidence="2">
        <location>
            <begin position="24"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="21908" checksum="96E5B952F0665A1F" modified="2014-01-22" version="1">MKIMRLVAALAVVAGVVLTASGPATLTGLLPYFTIQSNVAYGIFAVWSLSGRTTPPAWKGAVTLYVAITGLVYHLVLDNPASGFAAGPVERALPEAIGNQLLHTVVPLLAVLDWILFDERGRFRWRYALYWLAFPLGYLAFALIRGLIVHRYPYPFIDVRELGYDGVAISATGFALAFWLLGLLFVAIDRGSARLRRRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCG9</accession>
    <name>U5WCG9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LAO/AO transport system kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37205"/>
    <dbReference type="EnsemblBacteria" id="AGZ45695">
        <property type="protein sequence ID" value="AGZ45695"/>
        <property type="gene ID" value="AFR_37205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7527"/>
    <dbReference type="eggNOG" id="COG1703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043725_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="48"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="316" mass="32740" checksum="94B06E4077941946" modified="2014-01-22" version="1">MRTRDVPTLVARAREGDPRSVARLITLVENGDPKLAEVAAALAPYAGRAQVVGLTGSPGVGKSTTTNELVRALRKAGHRVGVLAVDPSSPFTGGAILGDRIRMQDHTTDPGVYIRSMSSRGQLGGLAAATPQAVRVLEGAGCDVVLVETVGVGQAEVEIASLADTTLVLLAPGMGDAIQAVKAGILEIADVFVVNKADRPGADATYRDIQGMLSLGERSPGEWRPQVVRAVAVKGEGVDDVVAAIEKHRAWLESSGRLQARREARAAAEVEAIALGTLRARLGSLREGTALSDLAARVAAGGLDPYAAAESLIKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDR4</accession>
    <name>U5WDR4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_38985</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562385.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38985"/>
    <dbReference type="EnsemblBacteria" id="AGZ46051">
        <property type="protein sequence ID" value="AGZ46051"/>
        <property type="gene ID" value="AFR_38985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7890"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_5_11"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="6"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="125"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="230"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="390" mass="43623" checksum="B476676E79E62E70" modified="2014-01-22" version="1">MDFSLSDEERAVRETARTFITREVMPLEQEALRRERAHQPGLERSELRELQLKAKKFGFWGLSTPEQYGGMDLPAVLQSLIWTEVGRTFVQFKFGGEADNILYYANDAQKAEYLVPTIEGDRISCFAITEPGAGSDAANIKLSARQDGDDWILNGEKTFITNGNEADFVIVVAVTDKEKGVRGGGTTAFLVDREMGWRSEFIQTMGEGGPAALIFDNVRVPARNILGEIGQGFELGMKWIGKGRYLIPSNALGIAERALGMAIEYANTRETFGARIGTNQAIQWMIADSEVELEAARWLILKAAWTVDQGADPRHASSMAKLYGANMVNNVVDRVMQIHGGMGYTRELPIERWYRQVRLMRIYEGTDEMQRLIISRDLLRGFTKIGGHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE65</accession>
    <name>U5WE65_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_40330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40330"/>
    <dbReference type="EnsemblBacteria" id="AGZ46320">
        <property type="protein sequence ID" value="AGZ46320"/>
        <property type="gene ID" value="AFR_40330"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40330"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8162"/>
    <dbReference type="eggNOG" id="COG4124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038234_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665810" description="GH26 domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="6">
        <location>
            <begin position="229"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="548" mass="58374" checksum="011531DDB5EF3C05" modified="2014-01-22" version="1">MTGVVAAGGGFALTGTANAATTTETTSTYSTDDAYTSSSRRTVNFGAADKLVVGREAGETRLSYVKFTPKIASGATVTAAQLKLPVESKPVAATLSVHSVASSWSEQKITAANAPALGALVASIKPSTTDATLTFDVSKVVTKAGTYAFALRSSATTAVTRLRSIEYGATTSGGPELVVTARRTTTTPTTAPTSTAPTKAPTTAPTTTPPTTTPPTTTPPTTTPPTTVPPTTAPGECVTDALLVPSCGVLWGAAAGGFTDTPRDQALKSWEAMTGRTATIYHTYHKGDEQFPTKPEIAMTQDAQNPRVLLLNWKIAYNSTWAKVAAGAQDARIDKWSAYVKKNYNQKFFLALHHEPENDVNATAGSGMTAKDYAAMYRHVITRLRTNGVTNAVNVIAFMGNEKWMAQSWWKDLYPGDAYVDWVGLDSYVSVEKGYYHYGDMGDILDRQPTGGGLGWYDWAVKNHPTKPIMVAEWGMYHRTKSITDKAAAFNTVIPELKAHPAVKAVVYFDTASDDEGDRDISVNSTASSLAAFKKVAADPMFKVTLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE70</accession>
    <name>U5WE70_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal RNA small subunit methyltransferase I</fullName>
            <ecNumber evidence="7">2.1.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">16S rRNA 2'-O-ribose C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">rRNA (cytidine-2'-O-)-methyltransferase RsmI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rsmI</name>
        <name evidence="9" type="ORF">AFR_39835</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(1402) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42924"/>
            <dbReference type="Rhea" id="RHEA-COMP:10285"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.198"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the methyltransferase superfamily. RsmI family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.198" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39835"/>
    <dbReference type="EnsemblBacteria" id="AGZ46221">
        <property type="protein sequence ID" value="AGZ46221"/>
        <property type="gene ID" value="AFR_39835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8060"/>
    <dbReference type="eggNOG" id="COG0313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044779_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070677">
        <property type="term" value="F:rRNA (cytosine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000453">
        <property type="term" value="P:enzyme-directed rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11648">
        <property type="entry name" value="RsmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01877">
        <property type="entry name" value="16SrRNA_methyltr_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008189">
        <property type="entry name" value="rRNA_ssu_MeTfrase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46111">
        <property type="entry name" value="PTHR46111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005917">
        <property type="entry name" value="MTase_YraL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00096">
        <property type="entry name" value="TIGR00096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="29877" checksum="52B42087BCB93801" modified="2014-01-22" version="1">MVLVGAPLGNVGDASTRLREVLATADVVAAEDTRRLLRLARDLDVTVAGRIVSYFEGNEERRTPDLVESLRGGAVVAVVTDGGMPSVSDPGYRLVRAALDAGFPVTAAPGPSAVTTALALSGLPSDRFCFEGFLPRTGSGRRSRLRELIAEPRTLVFFEAPHRITAALKDFAATFGADRDAAVCRELTKTYEEIRRGPLGELAEWSAEAGPRGEITVVVAGAPAGPAERPADEDLRAEVAEREAAGATRRDAIQAVADVHGLRKREVYALVHAEGSAPRTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNS5</accession>
    <name>U5VNS5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminodeoxyfutalosine synthase</fullName>
            <shortName evidence="6">AFL synthase</shortName>
            <shortName evidence="6">Aminofutalosine synthase</shortName>
            <ecNumber evidence="6">2.5.1.120</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Menaquinone biosynthetic enzyme MqnE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mqnE</name>
        <name evidence="8" type="ORF">AFR_01470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Radical SAM enzyme that catalyzes the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate, leading to aminodeoxyfutalosine (AFL), a key intermediate in the formation of menaquinone (MK, vitamin K2) from chorismate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-[(1-carboxyvinyl)-oxy]benzoate + H2O + S-adenosyl-L-methionine = 6-amino-6-deoxyfutalosine + H(+) + hydrogencarbonate + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:33075"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64286"/>
            <dbReference type="ChEBI" id="CHEBI:76981"/>
            <dbReference type="EC" id="2.5.1.120"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. MqnE family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.120" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357526.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01470"/>
    <dbReference type="EnsemblBacteria" id="AGZ38583">
        <property type="protein sequence ID" value="AGZ38583"/>
        <property type="gene ID" value="AFR_01470"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01470"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.295"/>
    <dbReference type="eggNOG" id="COG1060">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040406_0_0_11"/>
    <dbReference type="OrthoDB" id="940969at2"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102573">
        <property type="term" value="F:aminodeoxyfutalosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00993">
        <property type="entry name" value="MqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022432">
        <property type="entry name" value="MqnE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03700">
        <property type="entry name" value="mena_SCO4494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00423">
        <property type="entry name" value="TIGR00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="52"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ38583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="389" mass="44888" checksum="840F58C36BAB3192" modified="2014-01-22" version="1">MDVGLKRELEAKVYAGERLTREDGVALYESDDLAWLGRLAHHKRTEMNGDRVMFNVNRHLNLTNVCSASCAYCSFQRKPGEKDAYTMRIDEAVRKAKEMENEQLTELHIVNGLHPTLPWRYYPKVLRELKAALPNVKLKCFTATEVQWFEKISGLPASEILDELMDAGLESLTGGGAEIFDWEVRQHIVDHNCHWEDWSRIHRLAHEKGMKTPATMLYGHIEEPRHRVDHVMRLRELQDETGGFAVFIPLRYQHDFVDSADGKIRNRIQERTTMAAPAESLRTFAVSRLMFDNVPHLKNFWVMHGLSVAQLSLNFGADDLDGSVVEYKITHDADSYGTPTTMHRDDLLNIIWDAGFQPVERNTRYEIVREYAKPTSLAERRSEPQQVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPZ3</accession>
    <name>U5VPZ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell wall anchor domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_03670</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03670"/>
    <dbReference type="EnsemblBacteria" id="AGZ39023">
        <property type="protein sequence ID" value="AGZ39023"/>
        <property type="gene ID" value="AFR_03670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.739"/>
    <dbReference type="HOGENOM" id="CLU_978721_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665131" evidence="3">
        <location>
            <begin position="26"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="28542" checksum="6BBD3A30A76519AA" modified="2014-01-22" version="1">MKKLLGLGAAALAVGAMSFPAAAVAARAEGPALVALTEKDAATGKNKVYPVEQGDLSPAVIGVANNSEAAIDGVVVNIRVLNDLDLPRTFSNCAYYDDSNLEGAWCEFDTELAAGKTYAIADSLVKVAPDAVQENISAIIHHWITADAAKEQGGIEALAKRDSRTDESAPGTQGPVALEERELPLPGTLTPLGFAYVKLPPTPTTTPTATPTATPTATPTATPTGSPTATPTSTEGAAAGGGDGGGLPVTGAKAATVAGAGVALLLAGGAGYVVARRRRTRFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQG6</accession>
    <name>U5VQG6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7 8">Elongation factor Tu</fullName>
            <shortName evidence="7">EF-Tu</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tuf</name>
        <name evidence="10" type="ORF">AFR_04495</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358166.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04495"/>
    <dbReference type="EnsemblBacteria" id="AGZ39188">
        <property type="protein sequence ID" value="AGZ39188"/>
        <property type="gene ID" value="AFR_04495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.908"/>
    <dbReference type="eggNOG" id="COG0050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_0_1_11"/>
    <dbReference type="OrthoDB" id="621774at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="10"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="83"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="138"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="43720" checksum="F5C919BDABDAE38B" modified="2014-01-22" version="1">MAKAKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDQYPDLNPYTPFDEIDKAPEEKARGITISIAHVEYQTAARHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTKEHVLLARQVGVPYIVVALNKSDMVDDEELLELVELEVRELLSTYEFPGDDLPVVRVSALKALEGDAEWSGKLMELMNAVDTAIPQPERDVEKPFLMPIEDVFTITGRGTVVTGRAERGILKPNEEVEIVGIREKSTKTVCTGIEMFRKLLDEARAGENVGLLLRGIKREDVERGMVVVKPGSSTPHTEFEGQVYILSKEEGGRHTPFFQNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDSTSMSVKLIQPIAMEQGLKFAIREGGRTVGAGTVTKINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQQ5</accession>
    <name>U5VQQ5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heroin esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39164">
        <property type="protein sequence ID" value="AGZ39164"/>
        <property type="gene ID" value="AFR_04375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.884"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_3" evidence="1">
        <location>
            <begin position="88"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="34464" checksum="141F7DE7609C4D30" modified="2014-01-22" version="1">MPVPLDAELQVGYDAFLVEEPELAQMSLEMLAGIRAQVDAEVAALGDLSRGGRFTVSQPSAPGLAGAPEVPLLVCTPAGAPASRAVLYFMHGGGFFCNDHRIGLGQQLETAERFGATLISVGYRLAPEHPYPAQIDDAYAGLLWVADHGDELGIDPERIVVTGYSAGGGLGAALALTVRDKGGPRLLGQLLMAPMLDDRNDSASALQMDGIEFWNRSYSGFGWSSLLREVRGTADVPQYAAPARATDLTGLPPAFLDVGSAECLRDEIVDYASRIWQAGGKAELHVWPGGFHAFDREVPEARISNAAVAARRNWLERLLTTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQQ8</accession>
    <name>U5VQQ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dipeptide transport ATP-binding protein dppD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_05045</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358800.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05045"/>
    <dbReference type="EnsemblBacteria" id="AGZ39298">
        <property type="protein sequence ID" value="AGZ39298"/>
        <property type="gene ID" value="AFR_05045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1019"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_11"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="21"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="37516" checksum="88BC36D978CEF1FA" modified="2014-01-22" version="1">MTDIKAEIDVLPGLDPRAPLLEVTDLHVEFRTQYGISRAVNGANFLLSPGETLAILGESGCGKSVTAQAIMGILDSPPGFITKGEVKYRGLDLLKLPESQRRKVRANQIAMIFQDALSALNPVFTVGYQLAELFRVHRGMSRADAKRRSIELLDQVKIPAAKARIGDYPHQFSGGMRQRVMIAMALALDPEVLIADEPTTALDVTVQAQIMGLLAELQQQRNMGLILITHDMGVVADVADRISVMYAGKVVEEAPVYDIYARPAHPYAKALLESIPRLDMKGQELNVIKGLPPALTAIPKGCAFNPRCAYAQDVCRQDPAPPPYIVAPGRTARCHFWREVVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQY4</accession>
    <name>U5VQY4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">rpsM</name>
        <name evidence="8" type="ORF">AFR_04640</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358218.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04640"/>
    <dbReference type="EnsemblBacteria" id="AGZ39217">
        <property type="protein sequence ID" value="AGZ39217"/>
        <property type="gene ID" value="AFR_04640"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04640"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.937"/>
    <dbReference type="eggNOG" id="COG0099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_1_2_11"/>
    <dbReference type="OrthoDB" id="1772647at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019980">
        <property type="entry name" value="Ribosomal_S13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="PTHR10871:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03631">
        <property type="entry name" value="uS13_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="95"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="126" mass="14314" checksum="86FBC530CFD21164" modified="2014-01-22" version="1">MARLVGVDLPREKRMEIALTYIFGVGRTRAVEALTSTGIDLNKRAKDLTDEELVQLRDYIEGNFKVEGDLRREVAADIRRKVEIGCYAGIRHRRGLPVRGQRTRTNARTRKGPKRTVAGKKKPGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR07</accession>
    <name>U5VR07_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358939.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05595"/>
    <dbReference type="EnsemblBacteria" id="AGZ39408">
        <property type="protein sequence ID" value="AGZ39408"/>
        <property type="gene ID" value="AFR_05595"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05595"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1135"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_0_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="36"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="30642" checksum="7D771D86D6069784" modified="2014-01-22" version="1">MDAQRQLGEFLQTRRSQLRPEDVGVATYGGRRRVPGLRREELALLAGVSASYYSRLEQGQAANASPEVLDALARALRLNETERLHLDELATGSRRRTRAKRPAPEKLSEATRQLVAALGDVPVVVLGRRSDVLAWNPAGHSLFAGHLAADSKPNLAWLMFFDTHTRALYADWPAKARAVVATLRMASGRHPDDPLLAALVGELSVKSPEFVAMWADHRVKAGGDAVYEMLHPLVGPMRVTQQALWTEGGQAVVVATTEAGSPSQAAMTLLVHSRVTGPRLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRC0</accession>
    <name>U5VRC0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Subtilisin-like serine protease pepC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_06145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06145"/>
    <dbReference type="MEROPS" id="S08.037"/>
    <dbReference type="EnsemblBacteria" id="AGZ39518">
        <property type="protein sequence ID" value="AGZ39518"/>
        <property type="gene ID" value="AFR_06145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1248"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_13_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="3">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="27204" checksum="4030130FC5CA401E" modified="2014-01-22" version="1">MAGVIAGRGRSGGKGVLGIAPEAKILPTAPALGSLAVIKSLDWSVEHGAKVINMSFLVLEGDGLAAAIRRAADADVVLVAGSGNDEESETEDYEYPAAYPEVIAVGASDRNGKVASFSHQGPHLDLVAPGADVTVANGFPDDEYDQVEGTSVSAAIVSGAAALIRSEYPELSAAQVVEALESTAVDKGPAGRDDAYGNGELDLIAALDAAGKMKPQPVASAAASKPRTVPRSGSHSEDSGIPVALLAGAGVLVLAGLVAFMVVRRRARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRE2</accession>
    <name>U5VRE2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358913.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05465"/>
    <dbReference type="EnsemblBacteria" id="AGZ39382">
        <property type="protein sequence ID" value="AGZ39382"/>
        <property type="gene ID" value="AFR_05465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1108"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_18_2_11"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="18671" checksum="5F7AF953BB97F20B" modified="2014-01-22" version="1">MTVLLRPAADDDLPAVGALHFTSRAHAYAEILSPEALDHGSPEVMGEWWSERWKWEKETHRLTVAVDGGTLAGFSYLGPSEEEGAAELYAIHAAPEFVGTGVGRLLMLDALPALARIADHAVLWVLEPNTRARRFYERGGWVADGVTRTEPIGGEPVLQLRYGRAAGVVGGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSF4</accession>
    <name>U5VSF4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_08195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359459.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08195"/>
    <dbReference type="EnsemblBacteria" id="AGZ39928">
        <property type="protein sequence ID" value="AGZ39928"/>
        <property type="gene ID" value="AFR_08195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1672"/>
    <dbReference type="eggNOG" id="COG1295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045539_3_1_11"/>
    <dbReference type="OrthoDB" id="1421045at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017039">
        <property type="entry name" value="Virul_fac_BrkB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30213">
        <property type="entry name" value="PTHR30213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03631">
        <property type="entry name" value="Virul_fac_BrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00765">
        <property type="entry name" value="yihY_not_rbn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="39286" checksum="985F7C9F457F1BA4" modified="2014-01-22" version="1">MKLLRNADHSADDTKAPASGHAKDARTGEDKNLDAAATSRTAEDKAADLDAPSPDAGPESPAKLKGKGIMGALKRTFKQFSEDNVTDWAAALTYYGVLSIFPGVLVLVSILGMLSDNGQETVQQTVSEIAPSQIQTLLDQVLTQVSDPGTAGLAAIVGILAAFWSASGYIGAFMRASNAIYDVPEGRPIWKTLPIRLGVTAVIGLMLIVSVFIVVFTGDLAQAVGDTIGLGSAAVTTWSIAKWPVLVIIVSLMFAILYWASPNAKTGGFRWVSPGGIFAVVLWLIASGAFALYLASFANYNKTYGTLGGVIAFLVWLWISNIAIMIGAELDAELERGRAIAAGHDPTDEPFLQLRDDRKLKKGSEKGLSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSU4</accession>
    <name>U5VSU4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359470.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39939">
        <property type="protein sequence ID" value="AGZ39939"/>
        <property type="gene ID" value="AFR_08250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1682"/>
    <dbReference type="HOGENOM" id="CLU_2784532_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="68" mass="7604" checksum="415B53FC44291484" modified="2014-01-22" version="1">MLRRQGRHLLDQAEFQQTVGGSSQFVMCEGFESQGEDGEVKLLARLTLAREQDALACDRSAPYWVCVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTD5</accession>
    <name>U5VTD5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PMT_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">AFR_09075</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359654.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09075"/>
    <dbReference type="EnsemblBacteria" id="AGZ40104">
        <property type="protein sequence ID" value="AGZ40104"/>
        <property type="gene ID" value="AFR_09075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1846"/>
    <dbReference type="eggNOG" id="COG1807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_507913_0_0_11"/>
    <dbReference type="OrthoDB" id="1757243at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="PMT_2" evidence="10">
        <location>
            <begin position="66"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ40104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="527" mass="55567" checksum="038A124BD575751C" modified="2014-01-22" version="1">MLDAARRPILAAAVLAVLLRVPFLFTGLSTDEGGYAYVAQQWSRGDRLYDTAWLDRPQGLLLTYRALLAIDDSGWTIRLGMVLAGAIITVALAAIGWLLIGRATGIAAAWIYAVVGLAPHLEGITFNGELLASVPSTVAIALAVFWWRRDRSLWWLAGAGVLSGVALTMKQSGIDGIVVGLVILLLVARAARSVRPAPVSGPAGSPSSLSPASDPAGPAFHLLTGSEAARLPSASEGTAEPVPDGVGVLAAGGAFLGGVALPLLASVLHGIAVGWSYYWTALIGYQLSAMGGSGSNTGTRLTDLGRHAGDIALDLTVILIVSLLGWKAFDRGGRWLLGAWLFAGFVGINLGGSYWPHYFVQPLPALVLLVAAAVVGVRSVRWRRVLAAVVVLPTLGWLVTLAVMSPARRADTIPYDALAARDDRIAAVIRASTAPDDRIYVLESEAYLYFAADRQSPYPYLWGKPIDKIPDALPRLRAMLTSPQRPVLVVLDTPPAAVDPSGGIAADLAAHYHLDTVVEDVTILRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTF2</accession>
    <name>U5VTF2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Barstar (Barnase inhibitor)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the barstar family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359583.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40052">
        <property type="protein sequence ID" value="AGZ40052"/>
        <property type="gene ID" value="AFR_08815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1794"/>
    <dbReference type="eggNOG" id="COG2732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121832_2_2_11"/>
    <dbReference type="OrthoDB" id="2047621at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000468">
        <property type="entry name" value="Barstar"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035905">
        <property type="entry name" value="Barstar-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01337">
        <property type="entry name" value="Barstar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52038">
        <property type="entry name" value="SSF52038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Barstar" evidence="2">
        <location>
            <begin position="5"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="97" mass="11027" checksum="6D121A0DC48FE68E" modified="2014-01-22" version="1">MNVVIDGRFVLNEADLQQRLAGAFGYGPYYRHDLDDLRDHLAAGDPRPVQLVWIHAEAVRLALGAALFDSYVAVLEAIAAQDEERDWNDRFVFRLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTF5</accession>
    <name>U5VTF5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357965.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39020">
        <property type="protein sequence ID" value="AGZ39020"/>
        <property type="gene ID" value="AFR_03655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.736"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153137_1_1_11"/>
    <dbReference type="OrthoDB" id="1836278at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="123" mass="13547" checksum="B6FCF5F954FCECC0" modified="2014-01-22" version="1">MIDETSPALKVALAHHRAWTAKDLDLAMSYLADAFVCDAPAGRLGSRDAYREFLTPFSRMLISSTLIAGFGDQEQALIMYDTTTPLVASGPGAECLTVRDGLITYSRFLFDRLPFEQARQARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTK7</accession>
    <name>U5VTK7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357939.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03535"/>
    <dbReference type="EnsemblBacteria" id="AGZ38996">
        <property type="protein sequence ID" value="AGZ38996"/>
        <property type="gene ID" value="AFR_03535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.711"/>
    <dbReference type="eggNOG" id="COG3217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028286_4_0_11"/>
    <dbReference type="OrthoDB" id="1885467at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOSC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="114"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28364" checksum="EABC6690203EC5AC" modified="2014-01-22" version="1">MKLVALRRYPLKSALGEHLDAADVEPTGLLGDRTWACVDVSDGTIGSAKHPRRWGNLLEVATELHGSELTLRIAGRAHRAGSAEADAALSELVGRPVRLTQEAHAGAQLHRVASDVDGMIPEWTPDLRAGDESVSDVGGIARVGRFTDFGAIHLLTTGALARLGERLGGTAVADARFRANLILEAPDDPAPGDELRLGDVVLRIVMPTPRCVIPGLGQAGIPADRALLSTLARHYRRPVADFGRGACFGVYADVVQPGRLQLDQIAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTM0</accession>
    <name>U5VTM0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_09265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359730.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09265"/>
    <dbReference type="EnsemblBacteria" id="AGZ40142">
        <property type="protein sequence ID" value="AGZ40142"/>
        <property type="gene ID" value="AFR_09265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1887"/>
    <dbReference type="eggNOG" id="ENOG50331DK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129731_0_0_11"/>
    <dbReference type="OrthoDB" id="1937643at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="106" mass="12535" checksum="E32A9DB4EE0A00A6" modified="2014-01-22" version="1">MAWFRRRREPARGAGRQADRAGLEHLAEFVRSRRGVEGFIEPETTVTETTLLLVAHDGEWTRRRVDSPEGARRFAHQLAMPIYDIRLMGYPQRMRDYNERQKRRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTW5</accession>
    <name>U5VTW5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NmrA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359948.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09815"/>
    <dbReference type="EnsemblBacteria" id="AGZ40252">
        <property type="protein sequence ID" value="AGZ40252"/>
        <property type="gene ID" value="AFR_09815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2001"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_10_6_11"/>
    <dbReference type="OrthoDB" id="1616572at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="49"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="26129" checksum="B2DFF8778DFAEB8F" modified="2014-01-22" version="1">MILVTGATGNVGREAMRLLDGQGAGLSRSTPPPPFTGVEAVLYSPRAGTPELLTRAAEHGAKRVVVISAATVVQPVGDPRFIAEFTAAEEAARASGLEWTILHCADFASNTLAWAPQIRATGVVRGAYADAVTSTVDERDVAAVAVRVLQEAGHAGRAYLITGPQPLTQADKVRAIGAELTFEEVTPERVRQGMLAAGLPAEIPDRLLGSLADYARTPGPSSTTVAEVLGRPARTFAEWAADHAGVFSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTX9</accession>
    <name>U5VTX9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative subtilisin inhibitor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_09890</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I16 (SSI) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09890"/>
    <dbReference type="EnsemblBacteria" id="AGZ40267">
        <property type="protein sequence ID" value="AGZ40267"/>
        <property type="gene ID" value="AFR_09890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2017"/>
    <dbReference type="eggNOG" id="ENOG50333FU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121949_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023549">
        <property type="entry name" value="Subtilisin_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036819">
        <property type="entry name" value="Subtilisin_inhibitor-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00720">
        <property type="entry name" value="SSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55399">
        <property type="entry name" value="SSF55399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665204" evidence="3">
        <location>
            <begin position="28"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="SSI" evidence="4">
        <location>
            <begin position="90"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="174" mass="17938" checksum="3E7067E49A201D28" modified="2014-01-22" version="1">MIRIVTATAAAALLLTAGLATAGPASAAVGGSAQTGTGSPTAAMISAVAAKQADVAKKLTADQSAVDKAVKKPAKKKTELTLSYLADAGFATAVVLRCDPAGGAHPKKVKACQTLTRINGNPARLKPAEMMCTMEYAPITAEIKGTWQGRKVDWSRTFGNRCDMIRTTGVLMAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU94</accession>
    <name>U5VU94_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal subunit assembly factor BipA</fullName>
            <ecNumber evidence="2">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">GTP-binding protein BipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">bipA</name>
        <name evidence="4" type="ORF">AFR_10490</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Binds to ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. BipA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360213.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10490"/>
    <dbReference type="EnsemblBacteria" id="AGZ40387">
        <property type="protein sequence ID" value="AGZ40387"/>
        <property type="gene ID" value="AFR_10490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2138"/>
    <dbReference type="eggNOG" id="COG1217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017016_4_0_11"/>
    <dbReference type="OrthoDB" id="143460at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03710">
        <property type="entry name" value="BipA_TypA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00849">
        <property type="entry name" value="BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035651">
        <property type="entry name" value="BipA_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042116">
        <property type="entry name" value="TypA/BipA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006298">
        <property type="entry name" value="TypA_GTP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF8">
        <property type="entry name" value="PTHR42908:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01394">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="1 2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Tr-type G" evidence="3">
        <location>
            <begin position="5"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="622" mass="68488" checksum="313B1E236AF5CC2A" modified="2014-01-22" version="1">MQTRTDLRNVAIIAHVDHGKTTLVDAMLRQGSQSHARGEMADRAMDSMDLEREKGITILAKNTAISYQPADSDTPVTINIIDTPGHADFGGEVERGLTMVDGVALLVDASEGPLPQTRFVLRKALHAKLPIILIINKVDRPDARIKEVLDETYELFLDLDADEHQIEFPIVYACARDGIASMTQPKDGTVPEDATDLSVLFKTILETIPAPTYTEGAPLQAHVVNLDASNFLGRLALCRVHEGTIRKGQTVAWCKTDGTISSVRISELLITEGLERKPAESAGPGDIMAVAGIADIMIGETLADAENPIPLPLITVDEPAISMTLGTNTSPLVGKVKGAKVTARMVKDRLDKELIGNVSLRVLPTERPDAWEVQGRGELALAILVEQMRRESYELTVGKPQVVTRTIDGKVHEPVERLTIDAPDEYMGAITTLLSTRKGRMEELINHGTGWLRMEWLVPARGLIGFRTEFLTETRGTGILHHLFEGYEPWFGELRTRNNGSLVADRAGVVTPFAMINIQERGSLFVEPTTEVYEGMIVGENSREDDMDVNITKEKKLTNMRAASADNTEKVIPPRKLSLEQALEFCREDECVEVTPAAVRIRKVVLDQQARGRAASRRKHQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU95</accession>
    <name>U5VU95_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative phosphatidylserine decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_05005</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358359.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05005"/>
    <dbReference type="EnsemblBacteria" id="AGZ39290">
        <property type="protein sequence ID" value="AGZ39290"/>
        <property type="gene ID" value="AFR_05005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1011"/>
    <dbReference type="eggNOG" id="COG0688">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_658706_0_0_11"/>
    <dbReference type="OrthoDB" id="891720at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033175">
        <property type="entry name" value="PSD-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35809">
        <property type="entry name" value="PTHR35809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00164">
        <property type="entry name" value="PS_decarb_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="415" mass="43272" checksum="B062BDF89C932E18" modified="2014-01-22" version="1">MTPFPAVSTAPVTGVGPRAARALTAEFARHNAATTALVIAADHSSSVVAAAVEALLPGDTLTLVAGERSTADLLRDHITGLGSWISDRVRILDSLAEADPADVVILGEPLTGTAEETRAVLESLTKYLNEGAVVSVATPAVPGRTGGAAAELFRQSALFGVGSDLVTRNQPPLRVHKLRFSQADTAVAATLAPAYRPSSVPVTRTMHIDSNGVAAAGIALGLAAVARAARPKSKLWLLPAVLAAPVAAFFRDPERDVPADARSIVAASDGRVLSVERMTDERLGEGEFLRIAVFLSVLDVHVNRVPVAGRVTDYFVIDGGYANAMTAAAEHNVAAYTVLDTDHGTVAVAQRTGLIARRIVQRVPVGTLVARGERMGLIRFGSRTDVYLPADRAEAVVAVGDKVVGASSVIARWLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUU7</accession>
    <name>U5VUU7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Activator of Hsp90 ATPase 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359041.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06105"/>
    <dbReference type="EnsemblBacteria" id="AGZ39510">
        <property type="protein sequence ID" value="AGZ39510"/>
        <property type="gene ID" value="AFR_06105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1240"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1189229_0_0_11"/>
    <dbReference type="OrthoDB" id="1914312at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="23913" checksum="33F1246CAA00C770" modified="2014-01-22" version="1">MTEHSEPKPYDVEQPVAAGRDEVWDAVTQPPVLRQWFGWDHDGLDHEIQQIFVDEATLTSPERMGWPDSSFLEVTGDDDRSTVRAVRIGDPAADPVAFDAMEEGWRVFLAQLRFLLEERPEGLRRTVHLTGSARGRGVLALADGEALPLGTRTAQVMTADGQLVLVSGHEPLDSPEAGRIEVVVSTFGLGDDAFAEVRDDWTQRWAALAREAVVTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVN8</accession>
    <name>U5VVN8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07455"/>
    <dbReference type="EnsemblBacteria" id="AGZ39780">
        <property type="protein sequence ID" value="AGZ39780"/>
        <property type="gene ID" value="AFR_07455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1522"/>
    <dbReference type="eggNOG" id="COG1512">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129852_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033437">
        <property type="entry name" value="DUF5130"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17174">
        <property type="entry name" value="DUF5130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="17281" checksum="5F8D382BA0D0608A" modified="2014-01-22" version="1">MTVGEHQEVISGPGEQPLTALDHPGGPTLTENPELTMSQDGIDALDGPFTTRQLLRLDEALRVADQQTGLTFSVYIGDLEEPVREHAEQLHRQLKGAATAVLIAVSPNQRMLEIVTGPDARKRISDRDSKLAALSMAASFAGGDLAGGVLSGLDQLASHAGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVU8</accession>
    <name>U5VVU8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359302.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07410"/>
    <dbReference type="EnsemblBacteria" id="AGZ39771">
        <property type="protein sequence ID" value="AGZ39771"/>
        <property type="gene ID" value="AFR_07410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1512"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654941_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013431">
        <property type="entry name" value="Delta_60_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17164">
        <property type="entry name" value="DUF5122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665263" evidence="1">
        <location>
            <begin position="25"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="419" mass="43881" checksum="D8281F32EF54C51B" modified="2014-01-22" version="1">MRRRLFAATALTCAALLGATPASADMAQPAVVSTNPVDFTPHVLDGTVWALAVVGDTVIVGGSFTKVQNSSRKTTYARKNVFAYGLRDGEVRSFAPQVDAPVYALAAGGSNTVYLGGSFKTVNGTNQRGLARVSVAEGARISSFGARINWGDVRALTARGSRLYAGGTFSSINGVTRVGLARLDAGSGAVDTGFDARLSAPGLSRTRVEHFDITPDGSRLVAVGALLKVGSLYRTQIAMFDLTGSAATVTSWYTDAYKPECMKGFDTYLRQVKFSPDGKWFVVAATGRASSPEKLCDSAARFETGGNGRHNPTWVQRTGGDSLYAVAVTGSAVYLGGHQRYLDNPYGSDSKGPGPGAVSRVGIGAVSPSTGRALGWNPTRDRGVGVRAFHVVPGGLIVGSDTEKLGKEFHGRIGMFPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVX3</accession>
    <name>U5VVX3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14155"/>
    <dbReference type="EnsemblBacteria" id="AGZ41113">
        <property type="protein sequence ID" value="AGZ41113"/>
        <property type="gene ID" value="AFR_14155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2872"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="231" mass="24383" checksum="5BF875DDA185CB67" modified="2014-01-22" version="1">MNEIKNVVLVHGAFADGSGWRGVYDKLTGLGYRVTIVQNPLTSLEDDVAATTRALDRQDGPTILVGHSWGGTVITEAGVHPKVAGLVYVSALAPDAGETTAQQYEGFAATPDFVIDVVEDGFGFLNHDKFKAGFAADTSDADAAFLRDSQVPINMAVFATPVKNAAWRDKPTWAVIAADDKSFDQAMLQHMAKRAGAEITNVPASHALFITQAGVVADVIVKAAQNAGSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW78</accession>
    <name>U5VW78_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dihydroxyacetone kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_08505</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08505"/>
    <dbReference type="EnsemblBacteria" id="AGZ39990">
        <property type="protein sequence ID" value="AGZ39990"/>
        <property type="gene ID" value="AFR_08505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1731"/>
    <dbReference type="eggNOG" id="COG2376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017054_6_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004006">
        <property type="entry name" value="DhaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012737">
        <property type="entry name" value="DhaK_L_YcgS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02733">
        <property type="entry name" value="Dak1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="SSF101473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02365">
        <property type="entry name" value="dha_L_ycgS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51481">
        <property type="entry name" value="DHAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaK" evidence="6">
        <location>
            <begin position="10"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="DhaL" evidence="5">
        <location>
            <begin position="362"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="569" mass="56959" checksum="AE9EB39890414990" modified="2014-01-22" version="1">MIRVKKLINAADDVVAEALDGLTLTSAGIARLAGSTTALRADLDAVREAGHVTLLSGGGAGHEPAHAGYVGAGLLSGAVAGEVFTSPSVDAVLEAIRAVASPAGVLLIVKNYTGDRLNFGLAAELARAEGIEVATVVVADDVALAADAGAGRRGLAGTVLVHKVAGAAAAAGLPLAEVVRRAQRVADTVGTMGLALSACTVPAAGQPGFLLDQDEAEWGLGIHGEPGVERAALPPARDVAQRLVAAIADDRAITSGQRVVVLVNGLGATPPMELSIMADATARELRKRGITVERLWAGSFLTALDMAGVSLSLLPVDDELLAGLDAPTTAPAWPQTVTAPEGAIVPAPAPAASGTGTLTADDPVRRALEAVAETLISHRDELTELDREVGDGDLGISLARGAAAVLAESPDYPGEDGPAAVLRAVSASVRRSVGGTSGPLYAILLLRAASALEAAGPDDPGRGWAAALRAGADGIREVGGAEVGDRTMIDALVPAAETLQTQLASGAPASTALAAAIEAARAGVESTMSITARRGRSSYLGDRVIGHPDPGAYAVVLWLSSIASTLAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWG6</accession>
    <name>U5VWG6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_08585</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08585"/>
    <dbReference type="EnsemblBacteria" id="AGZ40006">
        <property type="protein sequence ID" value="AGZ40006"/>
        <property type="gene ID" value="AFR_08585"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08585"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1747"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_3_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ40006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="109" mass="11799" checksum="1A9A4CEF35B54EBC" modified="2014-01-22" version="1">MVEISPMGEIDVENAYEIKEAVAEKLANGRPQRIELDMHAVAFIDSVGISALVAAFQLASVSGVKLVVTRPSRFAHRQLWVTGLLGLFGAPEPCTDCPEPSREPLVSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWM3</accession>
    <name>U5VWM3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361467.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15630"/>
    <dbReference type="EnsemblBacteria" id="AGZ41408">
        <property type="protein sequence ID" value="AGZ41408"/>
        <property type="gene ID" value="AFR_15630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3174"/>
    <dbReference type="eggNOG" id="ENOG5032R4F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_860058_0_0_11"/>
    <dbReference type="OrthoDB" id="1340592at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666153" evidence="2">
        <location>
            <begin position="30"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="326" mass="32083" checksum="A41C82F4DDA4CF56" modified="2014-01-22" version="1">MRRTAAKLATATVVVTAAALTLAPGSASADLVTHCIGTGGAVTVPNDLFVPAGQSCALTGTTITGNVSVAAGANLVITGGRISGEIQVAADGYLDAGTTDIGGGIDLASGGYGVFLEDTGTGSITVRPKGSATIDSFLFVENAAIDGTVTANAGEVRLDLGTEVTGNVSTTGTYYTDVHDSFVDGTLSVLNSATGSVVCGSAIRGRSTFAGNLGGVQLGPNGALAGCASGGYWGRDVSITNTTGGVTVDDNIIDGKLTTTSNTPAARVAANNRIRGGVTGEQGTAAAKSSAAQRTVADRDAKIEQHAADRTSSAVQEADVAGPAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWT5</accession>
    <name>U5VWT5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DegT/DnrJ/EryC1/StrS aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_15905</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15905"/>
    <dbReference type="EnsemblBacteria" id="AGZ41463">
        <property type="protein sequence ID" value="AGZ41463"/>
        <property type="gene ID" value="AFR_15905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3229"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_7_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="385" mass="40731" checksum="63C333041E72F2A7" modified="2014-01-22" version="1">MVAVVAMTQRKIPVMIPLLGEEEALAAADAVRSGWVAQGPRVARFEQEFAATVGAAHGVAVSSCTTGLHLALVVLGIGPGDEVIVPSLSFIATANAVRYVGATPVFADVDLATGNLTVETVDTVRTPRTRAVIAVHQGGVPFDVAALRKAADGWGVGLVEDAACAAGSTAYGQPAGTGAAIAAWSFHPRKLITTGEGGMITLDDADRAVRLRRLREHGMNVSAADRHASAQPVLEAYLETAYNYRMTDIQAAVGLVQLGRLAGLVEQRRALAARYQELLADLDGLVPVRDPAYGQTNYQSFWVLINPEFGATRDEALAVLAERGVSARRGIMAAHLEPAYADVAPGPLPVTERLTRDSLILPLHHALTADDQDYIVGILRDLARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWX0</accession>
    <name>U5VWX0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PLDc_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_09605</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40210">
        <property type="protein sequence ID" value="AGZ40210"/>
        <property type="gene ID" value="AFR_09605"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09605"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1958"/>
    <dbReference type="HOGENOM" id="CLU_176001_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027379">
        <property type="entry name" value="CLS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13396">
        <property type="entry name" value="PLDc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="PLDc_N" evidence="7">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ40210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="78" mass="8812" checksum="FB0681514ED79E7B" modified="2014-01-22" version="1">MAKQSWVEVKEQLDELPPWQKAGALVVAPLEIILTVAAAVDLVRRPSAQIRGPKALWWLGIFVQPIGPIAYLKWARRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX57</accession>
    <name>U5VX57_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TPR repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361518.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15885"/>
    <dbReference type="EnsemblBacteria" id="AGZ41459">
        <property type="protein sequence ID" value="AGZ41459"/>
        <property type="gene ID" value="AFR_15885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3225"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004665_2_1_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="2">
        <location>
            <begin position="134"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="557"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="677"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="768" mass="83349" checksum="2B5CB16E0A3CDC33" modified="2014-01-22" version="1">MEHVSDPPDPGSARSLDDLVGCLRALKLFGGDTSYEVITRRVNARWQAEGRPAEELARRGTVVDCFKTGRRRINGDLVVAVVQALHDDAGYLAHWRQALRVILAETAAAAQVRVLDRLPDDIAVFTGRDAEIERLLAPDAAVRVLAGMAGVGKSQLAIHAGHLLMAQERFDTTLFVNLRGFHPDPGQPPAEPAAVLDGFLRLLGVSGHKIPHGLPARTALFRERLAGRRALVVLDNAADEDQVRPLLPDSTGSLTLVTSRRRLDGLEPVHIDVEPFTTDEAELLLTRSVPEVALGTDVAAYRRVALRCGHLPLALSVVAGQMVARDGWTVTDHADRLDERHRHRRLDSGVELALHLSYQHLPEARRTLLRHIAAHPGPDLDDHAAAALLGTGTETAAGHLHDLAGEYLVQQPVAGRYLLHDLVRAYAADRAQDEDRPADRRAALTRLFDHYVHSAAAAMDALYPAERHRRPELPPRPSGGPRLDDPKTALQWLDAERATLVAVCRHAARADWPTQAVHLAAILYSYLDNGGHPADAVIVHTEAQHAARLLGDGVAEATALTNLGVVCWQLGRYPEAIGHLNRALALFRDLGDGRGEARTLGNLGVVHSASGQGEISLTYQQQALDRFVQLGDRVGEANTLTNLGSVSVRLGRPEPAVEHNSRALAIFRELQHNGGEATALNNLGDAQTLLGHYAEAVDHYEQALAIFRDLGERYGETCVLNGLGHALTGQGKTDDAIGRHREALALATDIDKPEEQSRARTALAELET</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXB5</accession>
    <name>U5VXB5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Potassium/proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_10060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360044.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10060"/>
    <dbReference type="EnsemblBacteria" id="AGZ40301">
        <property type="protein sequence ID" value="AGZ40301"/>
        <property type="gene ID" value="AFR_10060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2050"/>
    <dbReference type="eggNOG" id="COG3263">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_9_1_11"/>
    <dbReference type="OrthoDB" id="1194802at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030151">
        <property type="entry name" value="NhaP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507:SF7">
        <property type="entry name" value="PTHR32507:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="5">
        <location>
            <begin position="403"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="503" mass="52621" checksum="78EC1C756FE68C73" modified="2014-01-22" version="1">MTAGLDFALLIGAAVLLVAVAAVRVSSRLGVPSLLVYLLIGMVIGEAGLGIRFDDAELTRTLGFCALIMIIAEGGLTARWSTLRPVLGLSATLATLGVAVSIAVVGVVVHFVLDLDWRLALLYGAVLSSTDAAAVFATLRVLRLPPRLVATLEAESGMNDAPVVLLVVLLSATAGMTHPWWYELLLVGYELAAGAAIGLTVGMVGRWLLRNAALPSAGLYPIAAVGLTFLAYAAGAVAHASGFLAVYVAGVLLGNGRLPHRRAILGFADGLAWVAQIGLFVLLGLLVSPDRLGRALVPALVVGFALVLLARPLSVWVSALLTRSGRRLGWRAQTFLSWAGLRGAVPIVLATIPLSSSAPGAEGLFDAVFVLVIIFTLVQAGTLAPVARRLGITAPAEAVEIHVEAAPLERMHADVLQIEIEPGSRLAGVHIDELRLPAGAVVTLVVRGGAGFVPGPDTRLRTGDDLLIVTTAAARDLAERRLRAVSRRGRLARWFGEEGHDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXP0</accession>
    <name>U5VXP0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16275"/>
    <dbReference type="EnsemblBacteria" id="AGZ41537">
        <property type="protein sequence ID" value="AGZ41537"/>
        <property type="gene ID" value="AFR_16275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3302"/>
    <dbReference type="HOGENOM" id="CLU_098533_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021323">
        <property type="entry name" value="DUF2927"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11150">
        <property type="entry name" value="DUF2927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666186" evidence="2">
        <location>
            <begin position="23"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="26280" checksum="F50BFCD8788A1777" modified="2014-01-22" version="1">MLLVLALAVTGCDAPDSPTAGGSTATSARPAPSPSPSPSVPPPVVKPKVSKDTLNYFYKIALGAEYGDKVNVVIKWAQPVVTIKVDGSVSGSSRKCLNRVISDFNALTETTDLRITGNPGADIKFHFAPVSRFKKLEPEYVPGNDGFFYVTWRGDHTISSSTVLIRTTGISSSIRCHLIREELTQTMGLMSDSTEHTDSVFYGRYTPAPTKYSALDKRLIKLLYGGAVKPGDGKKEVARAVVVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY19</accession>
    <name>U5VY19_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YceI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_11530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360601.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11530"/>
    <dbReference type="EnsemblBacteria" id="AGZ40595">
        <property type="protein sequence ID" value="AGZ40595"/>
        <property type="gene ID" value="AFR_11530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2350"/>
    <dbReference type="eggNOG" id="COG2353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071003_3_0_11"/>
    <dbReference type="OrthoDB" id="1767227at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007372">
        <property type="entry name" value="Lipid/polyisoprenoid-bd_YceI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036761">
        <property type="entry name" value="TTHA0802/YceI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04264">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00867">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101874">
        <property type="entry name" value="SSF101874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YceI" evidence="1">
        <location>
            <begin position="19"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="229" mass="25206" checksum="5E553341EE99293D" modified="2014-01-22" version="1">MSDRPTRVWNGVTIPDPGVYVLDEAHKRVGFHAQHMMVSPVRGEFVEATATIIVGHDPLQSSVTATIFSGSIDTNNADRDTHLRSPDFLDVEVYRTLEYRSTGVKWQGGNDAIFEWARLRNNRLNRRGAVADLPEAAGRSAGRFVVTGDLTIKNVTQQVDLQLTFGGARRDPYGRDIIGFSASAEINRESYGLVWNVALDSGGFLVGKKVHIEIAGEAIHQADQRPGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY72</accession>
    <name>U5VY72_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360708.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11830"/>
    <dbReference type="EnsemblBacteria" id="AGZ40655">
        <property type="protein sequence ID" value="AGZ40655"/>
        <property type="gene ID" value="AFR_11830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2409"/>
    <dbReference type="eggNOG" id="ENOG5032YNP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154638_1_1_11"/>
    <dbReference type="OrthoDB" id="1859412at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037079">
        <property type="entry name" value="AF2212/PG0164-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015018">
        <property type="entry name" value="DUF1905"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08922">
        <property type="entry name" value="DUF1905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="11051" checksum="0233FE130B84B3E1" modified="2014-01-22" version="1">MLIKFAAELWVWDARKSETWTFVTLPEDASEEIHELSEGLRRGFGSLRVRATIGTSKWTTSIFPGNGGKAYALPVKRAIRTAQSLDVGDVATVTVELIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY78</accession>
    <name>U5VY78_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_18375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362326.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18375"/>
    <dbReference type="EnsemblBacteria" id="AGZ41953">
        <property type="protein sequence ID" value="AGZ41953"/>
        <property type="gene ID" value="AFR_18375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3729"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="28"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="309"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="556" mass="57137" checksum="A8A4C8611516C0A5" modified="2014-01-22" version="1">MTVPHPAAAPRRALLRSAVRGHLPVLGLILAVALLGSVAEALTPAAIGAVLNAATDGEGLAAGPVGVLAVLIVVAIVASVWRYAASSRLRIRVAFQLKQRLGLAVAGRARAIGREVSPGELATAMTSDTDAIAGYPGALIQLIASVVAFAVVAGYLLTSSLLLGAIVLVGVPLLMWTTTRVAEPLEGRQREQRRLLGRLGDLGSDIALGLRILRSIGAEQTFRTRYRETSRQARTAGTGIAGTEALIEAAKLLLPGVLLILVTWVGARLVLRGSIEPGELVAFYAAAAYLVEPLGALATFVTTRARSRVAADRVLRALAVDPPGWPGTGTTASGDLRDGTTGVEAAEGEFTVVAIGDERDAGALGARLAGLDPVDALTTTIGGVPIVALDRSALRRLVRLHGAEPTLFSGTLRANVDPGAAAADAAVAGALATAVATDVVDALPDGLTTVVGAQARTLSGGQRQRVALARAVLDAPAYLILVEPTSSLDTVTEVEVCGRIRAQRAGRTTVVVTTSPAFLEAADRVVFLAGEPIGGTHAELLATSEDYRTLVARENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYB8</accession>
    <name>U5VYB8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Non-ribosomal peptide synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_18550</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18550"/>
    <dbReference type="EnsemblBacteria" id="AGZ41988">
        <property type="protein sequence ID" value="AGZ41988"/>
        <property type="gene ID" value="AFR_18550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3765"/>
    <dbReference type="eggNOG" id="COG1020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_0_12_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010071">
        <property type="entry name" value="AA_adenyl_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001242">
        <property type="entry name" value="Condensatn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020802">
        <property type="entry name" value="PKS_thioesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00668">
        <property type="entry name" value="Condensation"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00824">
        <property type="entry name" value="PKS_TE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01733">
        <property type="entry name" value="AA-adenyl-dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="963"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="2025"/>
            <end position="2100"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="998"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="4">
        <location>
            <position position="2060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="2375" mass="255332" checksum="BA8B8380C8815DCA" modified="2014-01-22" version="1">MEGVVMAAQNGLKDILPLAPLQEGLLFHSEYDTDATDVYVVQQAIDLEGDLDVGALQAACAALLRRHANLRAAFRHSGLKRPVQLIPHEVALPWSEVDLTHLPVEDRVSAADAAQHEDRLHRFDLARPPLLRYTLYRLGTDHYRFVLTNHHILLDGWSRPMLVRELLALYATGGDDSGLPRVRPFRDFLQWLGAQNRERALDAWRDALAGLPGPTLVAPRAQDRRPVVPGRLDRELPASTFTALTELARAQGLTLNTLVQGAWAVVLGSLTGQDDVVFGTTVSGRPPELEGVETMVGLFINTLPVRVRLDPREPLVAGLRRLQDEQLRLQPYQQLGLADVQRLAGPGELFDTTMVFENYPVDVGSGGADGFRGVRVTSSRNRDATHYPLALVVVARGSLKLRLDHQPDLVPETQAGAVLDRLERVLNALAKDPSVPLGRLALLSDAEHRAALALGHGRRREAAPGTVPAAFRAQAARTPGAIAVRADDTTLTYAELDEASDGLARHLAGLGVGAETPVALLMDRSASLIVALLGILKAGGAYLPLHPGDPDDRMRTVVADTGAPIILADPANAARAAGLGPRVVDPSATEGGPVPEPALSPGALAYLIYTSGSTGVPKGVAVTHHNVLELAADSAFRTGAHERVLIHSPQAFDASTYELWVPLLSGGTAVVAPPGKLDVAQLGKTLAEHAVTALWLTSGLFQLVADEAPESLAGVREVWTGGDVVPAASVRAVLDACPDVTVVDGYGPTETTTFAVRHRLAPGTPVPAEVPIGTALDNTDLRVLDAALRPVPPGVPGELYLGGTGVGRGYHHRPALTAARFAADPYGEPGARLYRTGDLVRWTETGVLEYLGRADHQVKIRGFRIETGEIEAVLARHDEVAQAVVTVWEPAPGDKRLAAYLVGARGGTLDTAGVRAHAEAVLPGYMIPAAFVVLDTLPLTGNGKVDRARLPVPELAGAPAGRAPRSPREELLCQLFAECLGVASVSIDDDFFALGGHSLLATRLVSRIRTVLGAELAIRGLFDAPTVARLTRALDTAGTAREPVTAADPRPDRVPASYAQRRLWFLHRFEGPSPTYNIPFALRLTGDLDTGALRAAFADLAARHEVLRTVLAEDADGPHQVVLDADRGAPVLTAEDVTEEELPDALARAAGYCFALDAETPLRAWLFTITPDQHVLLVLLHHVAGDGWSVPILARDLSTAYAARHTGTAPAWLPLPVQYADYTLWNRRLLGTEDNPGSVLSEQLAHWSRTLAALPEELGLPTDRPRPAVSTYRGDRVVFEVPRELHDRLAKLAQQCHASPFMVLQAAVAVLLSRLTGGTDIPIGTPVAGRTDDALDDLVGFFANTLVLRTDTTGNPAFRDLVARVRETDLAAYAHQDLPFERLVEELNPERSLARHPLFQVMLTFNNTARGGTALRLPGLRAEDLPGTAETAKFDLLFAFAERRDPAGRPGGLGASLEYSTDLFDHTTAESFVDRLLRLLTAVTTDPKQRIGALELLSEPERRLVTTGFNTTETEVGGRSLPESFEAQVARTPGLPAVEWPGAVLTYAELNVAANRLARLLAARGAGPESRVAALLPRSGELVTTMLATLKAGAAYLPVDPEYPPARIRAMLDDAAPDVIVTSRDLAARFPDDRTVVLEPGALDGFAGTDLTDADRTAALTPAHPAYVIYTSGSTGRPKGITMPAGATANLLEWHARELPGRPGARVAQFTAVSFDVSVQEMLSAVLTGRTLVVCDEDVRRDPVALTRWLRDSRIQELYAPNLVVDAVCEAATTRGITLPELTDLVQAGEALTLHPAVRRFHEQHPGCRLHNHYGPAETHVVTAYRLPERVEQWPATAPIGAPIANSAMRVLDPWLRPVPPGVPGELYIAGANLARGYLNRPALTGQRFVPDPLGAPGTRMYRTGDLASWDGDGNLRYLGRTDDQVKIRGFRVEPGEVEAVLTTLPGVTRAAVTVREDRPGDKRLIAYVVPAQDTAALHRELTGRLPAHLVPAVIVGLDELPLTPNGKLDRAALPAPGQSAEGRAPATPTERVVAELFAEVLGAATVGADDDFFALGGHSFLAARLVARLRERLDTEAPVRYVFEAPTVAKLAALLDGGPRDRRASGAVVLPLRTGGSAAPLFCLHPGGGFSWCYAGLVQHLDADVPVYGIQARGLDGRTTPASTMDEMVDDYLTEIRRIQPHGPYRLAGWSFGGLSAHALAVRLRHEGEEVELLALLDAYPPTPGADRLEVVEHEVVAHNMRALGFDFEEQELLGDQEAVLLRFRDFLRASDASLGHLDAHDILALKDVYVNNVRIMRAFRPQFFDGNILFVSAAKMSAQDRESRLNVHFWQPFIGGSVDVADVESTHGDLMTDPRHVASVGAILDSRLRSGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYN9</accession>
    <name>U5VYN9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DoxX family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_18655</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362382.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42009">
        <property type="protein sequence ID" value="AGZ42009"/>
        <property type="gene ID" value="AFR_18655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3787"/>
    <dbReference type="eggNOG" id="ENOG5032SPK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126433_0_1_11"/>
    <dbReference type="OrthoDB" id="2018019at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13564">
        <property type="entry name" value="DoxX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13076" checksum="F2D4018D7C868B32" modified="2014-01-22" version="1">MNLVLWIIAGLLAVVFLGAGVMKVLQPKEKLAESGLAWTESFSTGTIKLIGVLEILAAIGLILPPLLDIAPVFVPLAAVGLVLMMIAAAVTHGRRKEYPMIAGNVVLLLLAAVVAWGRFGPYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYQ9</accession>
    <name>U5VYQ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_18220</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18220"/>
    <dbReference type="EnsemblBacteria" id="AGZ41922">
        <property type="protein sequence ID" value="AGZ41922"/>
        <property type="gene ID" value="AFR_18220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3698"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="60"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="27730" checksum="4169C5E67104FD10" modified="2014-01-22" version="1">MQIGCIVITLFMGVPIYLIALAALSSRESLNTFPLPLLPTDVSGETMTTFLQSTGIVGGLLNSLTIGLSTVVLALLIGLPAGYALARYAFRGRNPYQLGLLLTRALPIVVLSVPLARIFLDTGLYDTTYAVTLLHTALALPTTILITSAIFLGVSREQEEAAMIFGASPLKAFVRVVVPQALPGIAASAMFTFVLSWNEVLGASILTLNRRTLPAQVLATLADSPLAFRFAGGLALVLPALIFIAVVRRYLLNMWGTTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYV5</accession>
    <name>U5VYV5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_19355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362515.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19355"/>
    <dbReference type="EnsemblBacteria" id="AGZ42143">
        <property type="protein sequence ID" value="AGZ42143"/>
        <property type="gene ID" value="AFR_19355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3929"/>
    <dbReference type="eggNOG" id="COG2169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_88_3_11"/>
    <dbReference type="OrthoDB" id="1268974at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003313">
        <property type="entry name" value="AraC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037923">
        <property type="entry name" value="HTH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02311">
        <property type="entry name" value="AraC_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51215">
        <property type="entry name" value="SSF51215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="3">
        <location>
            <begin position="190"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="34153" checksum="E46A8F73DF239C94" modified="2014-01-22" version="1">MAEPPVESSHAMLYFSDGSLAYAGHYLHEGQHPVHTHSFVEVAVMIGGAGVHHSLAGRQQLEVGDVVLLRPGVWHGYEECERLDLYNCCFSTELLQRELAWTRADPLLGYLLWTGPYSDQRRGILTARLSPEVLAEAVDHLDALDRLRFSPISRHRGDIIGRLSLFLSCLARAVVDGVEAGSGQTHPAVVDAMRMMEARPAHQWTLTELAEALHLAPGYLVRLFKSATGLPPMAYLSRHRVEVAAARLLHTDDTISRIGESVGWPDQNYFARRFRAHYGLSASAYRSRFTHNSVRLRSWDAAPQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYW5</accession>
    <name>U5VYW5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a quinol + H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = a quinone + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:39663"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the F420H(2)-dependent quinone reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362530.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19430"/>
    <dbReference type="EnsemblBacteria" id="AGZ42158">
        <property type="protein sequence ID" value="AGZ42158"/>
        <property type="gene ID" value="AFR_19430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3943"/>
    <dbReference type="eggNOG" id="COG3945">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114921_2_0_11"/>
    <dbReference type="OrthoDB" id="1693553at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004378">
        <property type="entry name" value="F420H2_quin_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04075">
        <property type="entry name" value="F420H2_quin_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00026">
        <property type="entry name" value="hi_GC_TIGR00026"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="138" mass="15253" checksum="DB8EA521FCDE5A7D" modified="2014-01-22" version="1">MASFNDTVIENFRANDGVVGGHWEGKTLLLLHTTGRRSGKEYVTPLVAAPDGDSYVICGSLGGAPKDPEWVANLEAADEPVTVELGKDTVQADQTVVRPGDDDWERLYGIWRAYWPDAADYETKTDRKFPVARLTVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYX2</accession>
    <name>U5VYX2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6493 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362422.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18875"/>
    <dbReference type="EnsemblBacteria" id="AGZ42049">
        <property type="protein sequence ID" value="AGZ42049"/>
        <property type="gene ID" value="AFR_18875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3834"/>
    <dbReference type="eggNOG" id="ENOG502Z9S7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016093_0_0_11"/>
    <dbReference type="OrthoDB" id="1365334at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045472">
        <property type="entry name" value="DUF6493"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20103">
        <property type="entry name" value="DUF6493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6493" evidence="1">
        <location>
            <begin position="164"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="879" mass="93745" checksum="FA75EA2C16A37B5E" modified="2014-01-22" version="1">MSLDGETLDRPLRSGDAASVAALLLAATEAERTALADWVETSLKSMRREDWWKVEDTTAGAYALAIIGCLSSPTRAATLLCRRDIRDRWDRVPAAAFLELVRIRELPWLGDLAVRLAGKLTIDGAWQGGWAFVAGLLRESGTLPPATESVLRGWLNTLHDGGADLTPLLRDDPFLDALLPAVFTIDGLGSELNHGRWNKETGEWDNAPRFPAAIVDLIAEGRLARGPILDATVDRLTRGDKPGFLRAFVMLHDLLEPTVDELARHTVAYVQLLPHSGPVIAALAQRALRTLDEAGRLDAETLCEAGGLVLLRKEKNLAKAQLSILDKAAGRDPGRAGEVLETIAVAFGHPALDIQDRALALIAKHLPLLDDPGRDSRLTHLAEAATALGGDLPARAAQLFGAPEPEAAPQVVLPLPPPLPTAEMPPPITSAVELAEQLVALLHERTAVRWEHVLAGLVSLPAAGLPEVLQPVLDRYPDLFVQRYGAWQPRLVYLGEAIRSVIAPHDHGTFWPRMISMAETAGPADSPAVILVLRISELAVRITQTPVPMLVATPTHVNGSLDARVLLTRLQRAEAEGWEPWPIDFDQALLRVPRVTDPEVRAAADALTSPRGREFAAWLAAGGLPDPLSVRLEQLSGKGTRAMRSEELVARRVVAELYPDPTDRLGLETQLVALERSPNPDYSSGSAKTPDVDTMVLPHHREVIAAWSLPGLAGHADQDGRGGGELLPLLADCTGPIGPAMALALAYVFGARHQTDRTAAVDAFLALVPTDGAVAAAVGAELGDLCGDGTIKLNRVVTALTDAHRAGATAAVWEVLAAALPPLLPRAPRGLPDLLELAAQTAGALGVTAELDGLADVASGPGTTRLVKEAKRLHTVLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZF5</accession>
    <name>U5VZF5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FtsX_ECD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362641.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19985"/>
    <dbReference type="EnsemblBacteria" id="AGZ42269">
        <property type="protein sequence ID" value="AGZ42269"/>
        <property type="gene ID" value="AFR_19985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4055"/>
    <dbReference type="eggNOG" id="COG2177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1347635_0_0_11"/>
    <dbReference type="OrthoDB" id="1653234at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040690">
        <property type="entry name" value="FtsX_ECD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18075">
        <property type="entry name" value="FtsX_ECD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX_ECD" evidence="2">
        <location>
            <begin position="95"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="196" mass="21010" checksum="7108E64A7D0A05FA" modified="2014-01-22" version="1">MDQNLRGLFAEALDDEPALPAGSLAEAAMTDGARLRRKRRLFAGATAAAVAATVVVALNVIAPGPDPAAQVEAGAALRVPVKPGCSTPADRRATDVSIFLTDDITTGQRDDLRAVLAHDPRVREFRFEDRKEAYQKFVELWKDSPDFVKSVGPAALPESFRVALAQQNAFPAVATMLQKQPGVQDVVGNLCSWEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZT3</accession>
    <name>U5VZT3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362771.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20635"/>
    <dbReference type="EnsemblBacteria" id="AGZ42399">
        <property type="protein sequence ID" value="AGZ42399"/>
        <property type="gene ID" value="AFR_20635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4184"/>
    <dbReference type="eggNOG" id="COG4102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032896_2_0_11"/>
    <dbReference type="OrthoDB" id="663130at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010869">
        <property type="entry name" value="DUF1501"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07394">
        <property type="entry name" value="DUF1501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="403" mass="42104" checksum="54AE3B87A8970ACA" modified="2014-01-22" version="1">MDTITRRKFLTASGVVAAGALAAGGSAYALRDILATSGQRDPGKILVLVTLYGGNDGLNTVIPYADPAYAAARPGLTYDEGQVLRLEDGFGLNPGLKGLHKLYGEDRLAIVRGVGYPKPDRSHFRSMDIWQTASPERPGTTGWLGRWLDTAGRDPRLAVSFEPVLPPVLAGATSAGATVPGGALTLPQGVTPPVLDGFGTPAAGESPAQARAAACFADLVRVQGLMTDVAAEAEDDDEPEGQATATGGQAPPLDQQLALVTRCVEAGAATRVYSVSLGGFDLHADEKTAQEALLAKLDKPLAAFVERMAGREVVVAVYSEFGRRVHANASDGTDHGTASSVFLLGDGIKGGLYGEAPSLTDLDDGDLKHTTDFRDVYAMLLADVLDADPGRILDGWDGRFDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W057</accession>
    <name>U5W057_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21235</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362891.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21235"/>
    <dbReference type="EnsemblBacteria" id="AGZ42519">
        <property type="protein sequence ID" value="AGZ42519"/>
        <property type="gene ID" value="AFR_21235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4306"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100874_3_0_11"/>
    <dbReference type="OrthoDB" id="1345242at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="147" mass="16203" checksum="32FD0366800E46F9" modified="2014-01-22" version="1">MSTHRPAARVVCLDAGNRVLLLHWSDPVGGNLVWEPPGGGVDDGESFLDAARRELAEETGLDPAAVQDRSVTVERDFRWRGKHYTGTEEFFLARFDEPGPALGRAGLMPDEQEALLGHAWLTPDELTDLPDLQPPHLAEIIRLLTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W077</accession>
    <name>U5W077_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-hydroxybutyryl-CoA dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362804.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20800"/>
    <dbReference type="EnsemblBacteria" id="AGZ42432">
        <property type="protein sequence ID" value="AGZ42432"/>
        <property type="gene ID" value="AFR_20800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4217"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_11"/>
    <dbReference type="OrthoDB" id="1498685at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="26340" checksum="58AEC50B68B52D38" modified="2014-01-22" version="1">MAQPTTLRVEELDDRVVVTLHRPEARNAINAAMIRELHEVCAGLEDNPRLLLLTGAEGTFAAGADIAELRERGRDQALQGINSRLFDRIARLPLPTVAAVDGYALGGGAELAYACDLRLASPGAVFGNPEPGLGILAAAGATWRLRELVGASVAKQILLAGRRLDADDAYRLGLVAEIVPGDELLKRAHSLLDRMSRNGTLALKLTKLVADAPGGHPVTDDLAQAILFESEDKRTRMDAFLAGERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0M2</accession>
    <name>U5W0M2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363207.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22565"/>
    <dbReference type="EnsemblBacteria" id="AGZ42783">
        <property type="protein sequence ID" value="AGZ42783"/>
        <property type="gene ID" value="AFR_22565"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22565"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4574"/>
    <dbReference type="HOGENOM" id="CLU_1105287_0_0_11"/>
    <dbReference type="OrthoDB" id="2229436at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="251" mass="26686" checksum="413DAA15B4DDDF7E" modified="2014-01-22" version="1">MSAYTVLLRLYPSDHPRDEMLGVLEENGRPWWREAPSLIAGAVRARTGEGQPPSLRWLYAARAAALMLLVAGTTTPLIDLHYGVELRTGMTVATWICAGLAAVAVMAGVRLVALGLTAAALVLSGMDQISTVAIAGYTLAAVLLLLPGRRLRVLNPLPVLLALSWSLDYTLPAVLRTALVVAVILWTVIDERILLAAGLALFAGLITASTEVAEVGDTRGLLIMAAWHLALPATLVAIAALLTQRRTRFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0Z8</accession>
    <name>U5W0Z8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_16395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16395"/>
    <dbReference type="EnsemblBacteria" id="AGZ41561">
        <property type="protein sequence ID" value="AGZ41561"/>
        <property type="gene ID" value="AFR_16395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3325"/>
    <dbReference type="eggNOG" id="ENOG502ZKY9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2406679_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="92" mass="10402" checksum="D79C92CBDB83D2C1" modified="2014-01-22" version="1">MTFDVGVNERHQVVFSFDKFWGALSIKVDGVNVVRTVRLASVDRVKAYDFVVGTQEQHHVRIEKHREVLFAGFRPQPVYAFVDGNLVAQGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1G2</accession>
    <name>U5W1G2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362103.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17685"/>
    <dbReference type="EnsemblBacteria" id="AGZ41815">
        <property type="protein sequence ID" value="AGZ41815"/>
        <property type="gene ID" value="AFR_17685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17685"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141550_0_0_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="149" mass="16096" checksum="A2FB13C5678037E3" modified="2014-01-22" version="1">MFRGFATISFYADDLAAARDWYAELLGQEAYYAFPPAPAAPAYVEFRVGDDEDELGFIDRRYAPPGAANAPGGAVMFWHVDDLQGTFDRLLKLGATEYQPITAHGDGEGFTTASVVDPFGNVLGIMHNPHYVELLASRVSSPSAASKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1H5</accession>
    <name>U5W1H5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362006.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17440"/>
    <dbReference type="EnsemblBacteria" id="AGZ41766">
        <property type="protein sequence ID" value="AGZ41766"/>
        <property type="gene ID" value="AFR_17440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3536"/>
    <dbReference type="eggNOG" id="COG3211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018570_0_0_11"/>
    <dbReference type="OrthoDB" id="796397at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008557">
        <property type="entry name" value="PhoX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05787">
        <property type="entry name" value="DUF839"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="699" mass="74227" checksum="F3F212C764169945" modified="2014-01-22" version="1">MTERPRLLPLLGQAGHSGRDSMTCLYRCGNACDHPVPNKSDNPYLGDVVNAEMSRRGVVRAGAVGALVLGLGGTAAATASPAAAAEVAPAMPGTLKKGKAGPLTFKAIPPNRLDNLIVPNGYDSAVVMRWGDPVLPGAPGLDVTKQTAKRQSAQFGYNNDFVGVLPLRGDRRALLVVNHEYTNEELMFPGFTSLEALTVDQVEVAMAAHGLSVVELERVDGTGQWQPVRGRHLPYNRRITALATSFAFTGPAAGSAWLRTAADPKGTTAIGTLNNCSGGVTPWGTVLSGEENFNQYFVGGDGAPEALKPKLARYGISTTARVPAGSRRWERAQERFDLAKHPHEAHRFGWIVEVDPFDPESRPRKHTALGRLKHEGANVIVAKNGKVVAYMGDDERFDYLYKFVSDKKYLPGDSAYARRHNLTLLESGTLYVAKVTGDSPAGEIDGSGKLPSDGAFDGTGTWIKLVSGNRSYVPGMTAADVLTFTRLAGDAVLATKMDRPEDVQPSLRTGKVYAAMTNNSNRGVGTNPGPDEANPRNANKHGHIFELVEQGGDNTAESFTWSLPIVCGDPADPSTYFAGYDKAAVSPISCPDNVAFDSAGNLWISTDGNALGANDGLFATAIEGSERGHLKQFLTVPYGAETCGPFITGDDRSVFAAVQHPGEITGASLEKPASTWPDGDFAKPAVVVTWRLDGGPVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1I1</accession>
    <name>U5W1I1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative epoxide hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_23060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363393.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23060"/>
    <dbReference type="EnsemblBacteria" id="AGZ42882">
        <property type="protein sequence ID" value="AGZ42882"/>
        <property type="gene ID" value="AFR_23060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4673"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019414_0_1_11"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665989" evidence="4">
        <location>
            <begin position="31"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="EHN" evidence="5">
        <location>
            <begin position="36"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="419" mass="45622" checksum="6BD6235CDAFB4606" modified="2014-01-22" version="1">MIPQPTRRQLLQGSLAVAASTVLVQVPADAAPATGIRPFRVAVRREQLADLHRRVAATRWPTRELVDDRSQGVQLATAQALARFWTNRYDWRRFEAKLNSLPQFKTEIDGVDIHFVHVRSKHRNALPLVMTHGWPGSIVELLETIGPLTDPTAHGGTAADAFHLVLPSIPGFGFSGKPGDLGWDTARTARAWAELMRRLGYPRYVAQGGDLGAAVTDNLGRLAPAGLLGIHLNLLVSALGSTAGLPTNTTEEKAAAAALQTFSASGKGYIVEQATRPQTVGYAVRDSPVALAAWMLDHDTDSYLKISHAFRGGRPSGNLTREHVLDNVSLYWLTGTGESAARAYWERTQSAGHTLPPVAVPVSFSAFPGEIFVAPRSWAETSYPTLSYYHQAGRGGHFAAWEEPALFTRELRAAFRSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1I6</accession>
    <name>U5W1I6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_23950</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363648.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23950"/>
    <dbReference type="EnsemblBacteria" id="AGZ43058">
        <property type="protein sequence ID" value="AGZ43058"/>
        <property type="gene ID" value="AFR_23950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4851"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_11"/>
    <dbReference type="OrthoDB" id="1439189at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="3">
        <location>
            <begin position="4"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="297" mass="31777" checksum="2CBB5C898AC9ADD0" modified="2014-01-22" version="1">MSDLEVRQLRYFVAVAEELHFGRAAERLGMAQPPLSRAIRELERQLGVQLLVRTTRQVALTPAGEVLLRDARIALDAVTAAARRARHAGCPAPALRLALKADYDAGLLPLLLDAYQEEEAALPVELLLGGRGEQVPALRDGRADVALLPVPFDATDLDVEELLTEPRVVAMAAADPLAAHSVLRLADLTGRTELDGSPAEEGGTRPADPNRPALDLAQIFNLIEVGTILWFLPASVAHRHPRPGIAYRPVVDLTPSTLALAWPQHSRSPAVAAFVRVATAAAGARTVPAPAEPTRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1P6</accession>
    <name>U5W1P6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_24335</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363723.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24335"/>
    <dbReference type="EnsemblBacteria" id="AGZ43133">
        <property type="protein sequence ID" value="AGZ43133"/>
        <property type="gene ID" value="AFR_24335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4930"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_40_0_11"/>
    <dbReference type="OrthoDB" id="1570162at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="20483" checksum="746ACAB08A07E722" modified="2014-01-22" version="1">MTSRAESAAATRQALLDAAAELLDDGGLEAVTLRAVGGRAGVTRGAPYRHFPDKESLLIAIGTQAWDRLGDRIHAARADPELSPADRLRGGLTALIGLARTQPHLYQLMFSNPPGDPAALARAAQRSQIEFLAIVAELVGEHDARRYGALLISSANGIAGMEASSQLTDPKWGGLSAEDLVDTLVDLIAARKPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Y2</accession>
    <name>U5W1Y2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_23820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363622.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23820"/>
    <dbReference type="EnsemblBacteria" id="AGZ43032">
        <property type="protein sequence ID" value="AGZ43032"/>
        <property type="gene ID" value="AFR_23820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4825"/>
    <dbReference type="eggNOG" id="ENOG5033D73">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_747283_0_0_11"/>
    <dbReference type="OrthoDB" id="1518891at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="370" mass="38107" checksum="5F884EA833A7F5B2" modified="2014-01-22" version="1">MGFRVEPSYLDGYSKQVQRAGDHAAAVKTYLGGHPPPQSMGEDGLHALIGPVKSALDKSMNAALSASDRVNKVLVSSGDGLSEAASHYRHSDAAAAARLDNTLPAATTGEPTGLEKQWAANVCGPSFGDSRAPEGRLTAPGDTEFSHPLGWMDNLSVSNWALKGFDWVFGFNPLDRVLESLLGDWQAIAKGGIALGRAGDAFGDIGYNVQGGAIAVRAGWEGRAADAAYHRFTGIAGDTASLGDPLREMSRQYSEIARGVWNTSEAVSGFIKGLLDAAIIAGIAAAAGTATAASGVGAVVGYGVAAAEVANMLKLWGQATAAISAIYGAVQMALGVVEGQIAKLDSITLPDNSASSGYRHPLVPDWVGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W203</accession>
    <name>U5W203_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bacterial regulatory s, tetR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24365"/>
    <dbReference type="EnsemblBacteria" id="AGZ43139">
        <property type="protein sequence ID" value="AGZ43139"/>
        <property type="gene ID" value="AFR_24365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4936"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096009_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="180" mass="19051" checksum="B0EF189532EAC641" modified="2014-01-22" version="1">MMRSTAEVRRATVLHEAVTVFARAGFHATPVTAVAAAAGISEAYVFRLFRGKLGLFVAAAEACYDRILAALEAGAESAASGATEEVLRAMGSAYADLIADRDLLLFQVQAQSVTDVPEIRAAVQEGYGRLATFVQGRSGASGPQLQQFFAYGQLCHLIVTADLDGIDQPWASALTAGFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W204</accession>
    <name>U5W204_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein lsr2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363627.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23845"/>
    <dbReference type="EnsemblBacteria" id="AGZ43037">
        <property type="protein sequence ID" value="AGZ43037"/>
        <property type="gene ID" value="AFR_23845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4830"/>
    <dbReference type="eggNOG" id="ENOG5032RKK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139818_0_0_11"/>
    <dbReference type="OrthoDB" id="370464at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024412">
        <property type="entry name" value="Lsr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042261">
        <property type="entry name" value="Lsr2_dimerization"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11774">
        <property type="entry name" value="Lsr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="116" mass="12505" checksum="5CD26BE7873E16BD" modified="2014-01-22" version="1">MAKTIVTLLTDDIDGSKADRTLEFGLDGVAYTIDLSDKNAGKLRKALDPFLAAASRIGRSNTSGRIASRSTAAAAPSRANREQNQAIREWAGRNGFEVSERGRIPSSVVEAFHSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2F6</accession>
    <name>U5W2F6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_24655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24655"/>
    <dbReference type="EnsemblBacteria" id="AGZ43197">
        <property type="protein sequence ID" value="AGZ43197"/>
        <property type="gene ID" value="AFR_24655"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24655"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4995"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="21573" checksum="3808FDE7BD898418" modified="2014-01-22" version="1">MSREAGIRLAEELGGSYHQADLADDDAAAGLVPEVLDTHGRLDVLVNNAGISWPVPHDDLEGLTAADWRRLLDVNLIAPWLLCTAALPALKETSGCIVNVTSHAGVRPKGGSIAYAASKAALNHVTKLLAAALGPEVRVNAVAPGLVDTPLTKDWTAAQELWKTVSPMRRAAKPQDVADLIAMLIDHDYVTGEVIVLDGGLNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W372</accession>
    <name>U5W372_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_26015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364198.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26015"/>
    <dbReference type="EnsemblBacteria" id="AGZ43467">
        <property type="protein sequence ID" value="AGZ43467"/>
        <property type="gene ID" value="AFR_26015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5274"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="4"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="150"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="226" mass="24480" checksum="00C14DE942692CEC" modified="2014-01-22" version="1">MTIRVLLADDQMLIRAGFKMIIDSEPEFEVVGEATDGRQAVDLARRTRADVILMDIRMPHMDGLEATRRIGADDNLAGVRVLVLTTFENDDNVVLALQAGASGFLGKGVAPADLLHAIRVVAGGEALLSPKATRGLVSRFLSRPETATAEPVSLDGLTEREREILVLVAHGLSNDDIAERLYVSPLTAKTHVNRAMTKLNVRDRAQLVVLAYRSGLVRPGEQLPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W389</accession>
    <name>U5W389_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase S8 and S53 subtilisin kexin sedolisin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_27060</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27060"/>
    <dbReference type="EnsemblBacteria" id="AGZ43673">
        <property type="protein sequence ID" value="AGZ43673"/>
        <property type="gene ID" value="AFR_27060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5484"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_15_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665505" evidence="5">
        <location>
            <begin position="33"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="6">
        <location>
            <begin position="86"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="534" mass="54161" checksum="D62E0CA558A13256" modified="2014-01-22" version="1">MRTPPLAAAALSAVLGATLGASVLVAASPASADPGPPPAPAPPGSGWLNQAGTTLLNTVIKGAGGDKADKDDPDDGDSRTGAALTGAGIGVALIDSGVSPVPGLNGRGKVINGPDLSFESQAPNLRYLDTFGHGTHMAGIIAGQDPATVVAGSRFKGVAPGAHIVNVKVASADGASDVSQVIAGIDWVVAHRNDKGLNIKVLNLSYGTESAQAATLDPLAYAVEKAWEAGIVVVVAAGNDGFGANAVTMPAADPDVIAVGAADPRGTDTRTDDTVASFTNRGNASRHPDVLAAGSSVVSLRSPGSYIDRTYPTARLSTTLDPGQRFLRGSGTSQAAAVVSGSAALLLEQRPGLKPDAVKQLLMSTADPIAGGDAYAAGSGQINIARAARTKTANGFKQLNLAATGLGTLEASRGGAYVYDSVTGTPLTGEKDIFGRAWNALSWAVASGRQKSWNGGTWNGSTWTGTAWGAAVAGEQTWAPSTWTGRSWAGSQWAGRSWSGATWSGRSWSAQTWTGRSWSGRSWSAAGWTGEPWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3E1</accession>
    <name>U5W3E1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_27310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560061.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43723">
        <property type="protein sequence ID" value="AGZ43723"/>
        <property type="gene ID" value="AFR_27310"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27310"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5534"/>
    <dbReference type="eggNOG" id="COG2270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_7_11"/>
    <dbReference type="OrthoDB" id="1594866at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="462" mass="48469" checksum="7682A3ECC06F705D" modified="2014-01-22" version="1">MLSALDRQRSVAPPGFSRWLIPPAALSVHLCIGQVYATSVYKNSFIAHFDAGQTSVGIIFSIAIVMLGLSAAVGGTWVEANGPRKAMFVSACFWAAGFLVAALGISTKQIWLVYLGYGVLGGIGLGIGYISPVSTLIKWFPDRPGLATGLAIMGFGGGALIASPLSRQLLSAYDSGYDPNVATSVASGGALVALFLTLGMGYFVIMMFGVFNVRVPPEGWEPEGFDPASVKEKPLVTLASVSAANAIKTRAFWLLWIVLFCNVTAGIGILEQASPMIQDFFRDNAGKSSVAVAAAAGFVGVLSLFNMAGRFVWSSTSDLIGRKRIYMVYLGVGMILYALLAAAGHAAVGLFVILAGVILSFYGGGFATIPAYLRDLFGTFQVGAIHGRLLTAWSAAGVVGPLIINGFLDAQGKPGTLTASAYQPALFTMVGVLAVGFIANLLVKPVPERFHESRTEEKEAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3I9</accession>
    <name>U5W3I9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362878.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42506">
        <property type="protein sequence ID" value="AGZ42506"/>
        <property type="gene ID" value="AFR_21170"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21170"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4292"/>
    <dbReference type="eggNOG" id="ENOG5033A88">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084427_1_1_11"/>
    <dbReference type="OrthoDB" id="1605258at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014942">
        <property type="entry name" value="AbiEii"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08843">
        <property type="entry name" value="AbiEii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="23615" checksum="EFA7D78ADB9C7445" modified="2014-01-22" version="1">MSSNIDDFYRDVTTIALGVGEKHGFVLGGGVAWLVNGLVQRPTEDIDLFTDTAGAVGAAAGQVTDALTAAGYQVFREVADEMFEGMDEDLREYHVADGSRALRLTLTRLDRRRAPVVMDVGPVMHLDDLVASKVAALVNRREVRDYIDVAAALDRYPLARVIELARVLEPGLDDEDIADAGRYLDRLDDARFALYGLTPERVRALRERLAGWPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Q7</accession>
    <name>U5W3Q7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364561.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26955"/>
    <dbReference type="EnsemblBacteria" id="AGZ43652">
        <property type="protein sequence ID" value="AGZ43652"/>
        <property type="gene ID" value="AFR_26955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5462"/>
    <dbReference type="eggNOG" id="COG1819">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000537_8_0_11"/>
    <dbReference type="OrthoDB" id="1485440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033072">
        <property type="term" value="P:vancomycin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="1">
        <location>
            <begin position="3"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="416" mass="44091" checksum="EF12ECD1F1248E2C" modified="2014-01-22" version="1">MRVLIVTSGSTGDVAPYTGLGARLREAGHEVTLATHEPFRDTVELPFVPLPGDLREILPQARGQDGSGSGTGPRALLRLMRIARPLVAELGDGIVRAVEQTRPDAMLLSTVVAPLGYQVAEAAGIPWAGVFLQPVFPTGDFGPVLIGGRPLGRLGNRLTGRFLESAAHPLYARPVRDLRRRLGLPQRGMAALQRAQQHRWPTFHGFSPTVVPRPADWPASQEVVGYWWPERPSGWTPPREVEAFLAAGPAPVFIGFGSMAAGRGERLAAPVLEAVTKAGVRAIVQAGWSGLTVAGGPDVLSVGPMPHDWLFPRMAAVVHHSGAGTTAAGLRAGVPAVPVPVLADQPFWARRLRELGVAPAGVPLPRLTADRLAGALRAATTDPRHAARAQEVAAALRTEDGAARIIAWLKTQQPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W444</accession>
    <name>U5W444_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_28215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560241.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28215"/>
    <dbReference type="EnsemblBacteria" id="AGZ43904">
        <property type="protein sequence ID" value="AGZ43904"/>
        <property type="gene ID" value="AFR_28215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5719"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_11"/>
    <dbReference type="OrthoDB" id="935411at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="215"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="415" mass="43746" checksum="A5611B66BA3A2A80" modified="2014-01-22" version="1">MTGSPLDRLRRLPWWLAGWLTAITAVGTIVLAALLIQADTARGEAELDGRLGRVTAAVTRLISYDGTIVTAAVGTDEVNTGCPEFAVLPGPTERFAGHVSRRDCVPVDPGVAARLAAESVGTGRELTGYVRDANGDRARVRVEPFRNTRGQYVGAVVAMDDPAEFHAEHRRFVLLVCAGGLLFLAAVAFAGYVLADRAVRPASAALEQQEVLLADTAHDLRTPVAALRALAETALLQPDQRTELLPRAVDLARRMGTIIDGVLMRARLAAGVESLEVEPLWLDQLVSTVVEETLPGGAEITLTAAPSKVQADAGLVRRAITNLLDNALRHGRVPGQPAIVHITVAGGRVTVADHGPGIDAATAGESFERFKSSGGSSGLGLSIVRWVAQAHGGTLRVYNAEEGGAIFELELPLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W477</accession>
    <name>U5W477_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_22575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363212.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22575"/>
    <dbReference type="EnsemblBacteria" id="AGZ42785">
        <property type="protein sequence ID" value="AGZ42785"/>
        <property type="gene ID" value="AFR_22575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4576"/>
    <dbReference type="HOGENOM" id="CLU_1192730_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="232" mass="25047" checksum="5F557149AFC78CEB" modified="2014-01-22" version="1">MTPEYGWWIRETYRLTLLGVQTATVEAEATVARLTEQLDRVARAAPPPPPGTTLSAALNAECEDLAQRPAGLPGSLAAGYQAGRAAFLNGLRARASNVRRAGPDDARPYPGSDEPPGKPAEAYGYYRFLIAKTVPGVPEAAERQLAEAQAALNVLEQRILELRARQSRYAARISAADARWRNALAAHLRAHADEVAARSGYTDAFTIGYNLAADSLARRLRQRADEIAVPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4D3</accession>
    <name>U5W4D3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Myo-inositol-1(Or 4)-monophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_22900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363356.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22900"/>
    <dbReference type="EnsemblBacteria" id="AGZ42850">
        <property type="protein sequence ID" value="AGZ42850"/>
        <property type="gene ID" value="AFR_22900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4641"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_0_2_11"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="27277" checksum="E64849CF9BEBA050" modified="2014-01-22" version="1">MTTLLQDVEIVVKEAGAHLLRRYSAGSRQDSFEDIVSSVRANDSAVLAILRPGLTAARPDAGWVDDEHASGPMPAGEWWVVDPVGGNVNTIHGLRDWNIGVTLVRDQQPVLAVLYSPLGDELYTAEAGAGAYLNGVRLTTSAKTGLDAALVGTGQAKPGLDPRNAALLGASVTAMLQAALYVRVSVPVTHQLSQVAAGRMDLHWQFDNVRSHLAGLLLIKEAGGVVTDLDGRPWDLTSEHYLAATPGLHAAALKVLAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4G7</accession>
    <name>U5W4G7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TrmB family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28230"/>
    <dbReference type="EnsemblBacteria" id="AGZ43907">
        <property type="protein sequence ID" value="AGZ43907"/>
        <property type="gene ID" value="AFR_28230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5722"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077128_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="136"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="207" mass="22165" checksum="2B4A120F3D30ABE2" modified="2014-01-22" version="1">MTTVTPPHPLLLSTPAAIDAALANAGREVLVARTGPGEPLRRADHDNLRRGVRYRVLLPESVRAHPAVVLRLGRLVAAGAEIRTVAQVPADVTVVDETVAMMSLDRSAADVAVVHLPAVVDTVVALFARQWEVAAPLTSTDELCARERELLALLSSGYTDESAAARLGVSVRTVRRMMSVIMGRLGARSRFQAGLRAADRGLLMPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W579</accession>
    <name>U5W579_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29530"/>
    <dbReference type="EnsemblBacteria" id="AGZ44167">
        <property type="protein sequence ID" value="AGZ44167"/>
        <property type="gene ID" value="AFR_29530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5986"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077370_0_0_11"/>
    <dbReference type="OrthoDB" id="1417316at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="19525" checksum="94BC49A724E548EA" modified="2014-01-22" version="1">MISWRKGLRGAIFHELTERGWIRARQEFVVAPPERVSSAWLLHYATLRHLASIMERNDHKIADVFAATDPGPETAPSGKTEDTPLEDRIKRAYSEVAESPGDLVSLFSIRERLADFDRAELDRALKEMDRRREIHLDPDPRRNELPQEVLDAAIAVGGEDKHLLTIGQANGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5A9</accession>
    <name>U5W5A9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560738.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30715"/>
    <dbReference type="EnsemblBacteria" id="AGZ44403">
        <property type="protein sequence ID" value="AGZ44403"/>
        <property type="gene ID" value="AFR_30715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6219"/>
    <dbReference type="eggNOG" id="ENOG5033A8E">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167343_2_0_11"/>
    <dbReference type="OrthoDB" id="2087009at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="101" mass="11098" checksum="3597819406A3A6C6" modified="2014-01-22" version="1">MKRLLWLGVGLAVGAIVVRKVTRKANEFTPSGIATSLSESAGGLVESLRSFVEDVREGMAEREQEIHEAFAAGELYDDKFAELREDAQEGTHGRHAQEGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5F3</accession>
    <name>U5W5F3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Alanine dehydrogenase</fullName>
            <ecNumber evidence="3 6">1.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">AFR_29880</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible reductive amination of pyruvate to L-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + L-alanine + NAD(+) = H(+) + NADH + NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:18405"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="1.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AlaDH/PNT family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.1" evidence="3 6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560574.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29880"/>
    <dbReference type="EnsemblBacteria" id="AGZ44237">
        <property type="protein sequence ID" value="AGZ44237"/>
        <property type="gene ID" value="AFR_29880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6052"/>
    <dbReference type="eggNOG" id="COG0686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003376_3_0_11"/>
    <dbReference type="OrthoDB" id="673280at2"/>
    <dbReference type="UniPathway" id="UPA00527">
        <property type="reaction ID" value="UER00585"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000286">
        <property type="term" value="F:alanine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05305">
        <property type="entry name" value="L-AlaDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008141">
        <property type="entry name" value="Ala_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008142">
        <property type="entry name" value="AlaDH/PNT_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42795">
        <property type="entry name" value="PTHR42795"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01262">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000183">
        <property type="entry name" value="Alanine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00518">
        <property type="entry name" value="alaDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00836">
        <property type="entry name" value="ALADH_PNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 9" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AlaDh_PNT_N" evidence="12">
        <location>
            <begin position="4"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="AlaDh_PNT_C" evidence="11">
        <location>
            <begin position="149"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="239"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="267"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="298"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000183-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000183-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000183-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000183-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ44237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="38951" checksum="08E0EEBAA7CE9175" modified="2014-01-22" version="1">MKVGIPSEVKNNEFRVAITPAGVFELNRAGHEVFVQAGAGAGSSITDADYTAAGATILEGPDAVWATGDLILKVKEPIAEEYARMREGQLLFTYLHLAASKECTDALLDRRVTAIAYETVELPDRSLPLLAPMSEVAGRLAPQVGSYHLMRSGGGRGVLMGGVPGVYAAKVVVIGAGVSGMNAAAIALGMQAEVLVLDKNIARLRAADADYRGHLQTIASNAYEIEKAVIDADLVIGAVLVPGAKAPNLISNDLVSRMKPGSVLVDIAIDQGGCFEDSRPTTHADPVYKVHESMFYCVANMPGAVPHTSTYALTNVTLPYALELANLGWRDAIKADPALALGLNTHDGHVTYGPVAEAHGMQALSLAEVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5L5</accession>
    <name>U5W5L5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative sugar ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_30870</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560769.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30870"/>
    <dbReference type="EnsemblBacteria" id="AGZ44434">
        <property type="protein sequence ID" value="AGZ44434"/>
        <property type="gene ID" value="AFR_30870"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30870"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6249"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_3_11"/>
    <dbReference type="OrthoDB" id="1619224at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="70"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31673" checksum="95B5ABAC21069B1D" modified="2014-01-22" version="1">MRSAGRTPLWLLAPAAVVLGALFVYPMWQLGLLSVLDFRQAQVSGGQPARFVGTGNYAQLLGDTQFWSVLNATVLFAAACVVATLAVGAGLAVLLTRISRWPRLLLSLAAMAAWAVPAVSGSTVWMFLFDTDLGLVNQTLGLSGLNWMYDRYSAFALVGAVVVWHSFPFVMITLYAGIEAIPRSVLEAAAIDGASAWRTFWRIMVPILRPLLAIVVIQSIIWDFKVFTQIYVMTRGGGLAGQNLTLNVWAYQQAFAAGEYGLGAAIGVVMMVILLIVTLLYLRTLRRSGEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6R3</accession>
    <name>U5W6R3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative TraR/DksA-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_26745</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364478.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26745"/>
    <dbReference type="EnsemblBacteria" id="AGZ43611">
        <property type="protein sequence ID" value="AGZ43611"/>
        <property type="gene ID" value="AFR_26745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5421"/>
    <dbReference type="eggNOG" id="COG1734">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043144_3_0_11"/>
    <dbReference type="OrthoDB" id="1932026at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020458">
        <property type="entry name" value="Znf_DskA_TraR_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01258">
        <property type="entry name" value="zf-dskA_traR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01102">
        <property type="entry name" value="ZF_DKSA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51128">
        <property type="entry name" value="ZF_DKSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DksA C4-type" evidence="6">
        <location>
            <begin position="63"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="dksA C4-type" evidence="4">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="139" mass="15100" checksum="844784A80710ECAF" modified="2014-01-22" version="1">MLVNSAVITGKGADTKGRSSDEIEQIRGLLRTRFDDLTAEYEEAVAENHQLRLVEIGDAAGDDQADSGSKTAERDAASSLIRTLLDRRTQAEHAMQRLEDGTYGNCEGCSKPIPVERLTVFPSATSCVACKSNRERRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6R5</accession>
    <name>U5W6R5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561029.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32195"/>
    <dbReference type="EnsemblBacteria" id="AGZ44697">
        <property type="protein sequence ID" value="AGZ44697"/>
        <property type="gene ID" value="AFR_32195"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32195"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6517"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_1_2_11"/>
    <dbReference type="OrthoDB" id="2089619at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041467">
        <property type="entry name" value="Sco4008_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17926">
        <property type="entry name" value="TetR_C_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="20378" checksum="5D900E5CC97742DB" modified="2014-01-22" version="1">MARDAEDTKRRLLEAAANEFAERGIAGARVDRIAAAAAANKALIYSYFGNKEDLFDAVFNALVVDTVEDVPIDADDLPGYAGRLFDHGQEQPQVLRLALWHSLERGGAAMPEAVVAANLGKVEAIAAAQRDGRITDRFPAGELMVLITGLSILGAPDLALTHFDGPEGLAERRRTVVESVRILITKDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6Z0</accession>
    <name>U5W6Z0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycerol kinase</fullName>
            <ecNumber evidence="5">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycerokinase</fullName>
            <shortName evidence="5">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glpK1</name>
        <name evidence="5" type="synonym">glpK</name>
        <name evidence="9" type="ORF">AFR_27445</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Inhibited by fructose 1,6-bisphosphate (FBP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560088.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27445"/>
    <dbReference type="EnsemblBacteria" id="AGZ43750">
        <property type="protein sequence ID" value="AGZ43750"/>
        <property type="gene ID" value="AFR_27445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5563"/>
    <dbReference type="eggNOG" id="COG0554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_11"/>
    <dbReference type="OrthoDB" id="233605at2"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00186">
        <property type="entry name" value="Glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5 6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="7">
        <location>
            <begin position="5"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="8">
        <location>
            <begin position="257"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="241"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="408"/>
            <end position="412"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ43750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="497" mass="54269" checksum="5A3CCE4F90DB6FB1" modified="2014-01-22" version="1">MTERYVVAIDQGTTSTRCIVFDRHGQLVSLAQQEHKQYFPRPGWVEHDAAEIWRNVERLAPLALSRAGITTEQVAAVGIANQRETTVLWDRRTGVPVGRAIVWQDTRTDGLVNALVAAPGADEVQERCGLPLATYFSGPRVSWMLDHTPGLRERAESGEVLFGTMETWLIWNLTGGLHVTDVTNASRTMLLDVRTLRWSPEACAFFGVPMAMLPEVRPSVEVFGTATAAFPGVRIGAALGDQQAALFGQTCFTPGEAKCTYGTGSFLLLNTGTELVRSTHGLLSTVAYQLAGEPAQYALEGSIAITGSLVQWFRDQLELIASAPEIETLARTVSDNGGCYIVPAFSGLYAPHWRSEARGVIVGLTSYITKGHLARAVLEATAWQTREVVDAMNANSGLTLKTLKVDGGMTADNLLMQFVADVLDVPVVRPLASETVSLGAAYAAGLAVGYWSDLEDLSRNWHRAAQWMPAMDPAVRDSEYDNWRRAVERTFDWIKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7F6</accession>
    <name>U5W7F6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560267.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28345"/>
    <dbReference type="EnsemblBacteria" id="AGZ43930">
        <property type="protein sequence ID" value="AGZ43930"/>
        <property type="gene ID" value="AFR_28345"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28345"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5746"/>
    <dbReference type="eggNOG" id="COG4447">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_711031_0_0_11"/>
    <dbReference type="OrthoDB" id="1303819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666152" evidence="1">
        <location>
            <begin position="23"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="389" mass="41267" checksum="3427E48035080251" modified="2014-01-22" version="1">MSSLSTVLLALLLTGQPVVAERDSPWTPVPTPNPSMATNRLLGTTAIGDRAAWSVGTWQDNLTGRQRSLIQRWDGATWQNVDAPATRTGHDSLTAIAGSGPADLWAVGFTARSKHDPDKPADPDQGRPLIMRSDGITWAFEDLDPVGVSGHLSAVDMISADDGWAVGSYVPQEGAVPQGLILRRTRAGWQHVPPPDLGSRPLHLTGLTGTRTDDVWAVGYLGTLGSAAEAVVLHWNGEKWQQSPLDIDPGVQSALFSVSARAPGDVWAVGYTWAAGKRTGFALHHTAGRWQQVPVRVDEQGTQLRSVVAVPAGVWAVGYYLVDGVDNALVMTYDNTGFVKEAPPSPPEALNVAGTAMAGITATPGTGGLWAIGCHGDRTRVLHRDTGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7U1</accession>
    <name>U5W7U1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="3 9">2.7.1.180</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_34020</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ApbE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="3 9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34020"/>
    <dbReference type="EnsemblBacteria" id="AGZ45062">
        <property type="protein sequence ID" value="AGZ45062"/>
        <property type="gene ID" value="AFR_34020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6886"/>
    <dbReference type="eggNOG" id="COG1477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044403_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="5 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ45062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="249" mass="26785" checksum="B62596D9586417F3" modified="2014-01-22" version="1">MHHAEHVMGTVVSIDLADDLPDQTLRAMIGDVCGWLHEVDHRFSTYRDDSEVSRLRRRELKPSECSADMRHVLEACADLWRETDGYFDAYAAGPLDPSGYVKGWSVEIASARLAEAGSVNHCVNAGGDIRARGRSGQGLPWRVGIRHPWEADKLSWVLSLTDEAVATSGTYERGDHVFNPRTGGPARGLRSVTVVGPDLALADAYATAALAMGEPGIAWLAKRVADGYNSAVVTDDGRAFSSAGLPAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W850</accession>
    <name>U5W850_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ricin B-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560433.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29175"/>
    <dbReference type="EnsemblBacteria" id="AGZ44096">
        <property type="protein sequence ID" value="AGZ44096"/>
        <property type="gene ID" value="AFR_29175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5911"/>
    <dbReference type="eggNOG" id="COG3533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008033_2_0_11"/>
    <dbReference type="OrthoDB" id="194265at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012878">
        <property type="entry name" value="Glyco_hydro_127"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07944">
        <property type="entry name" value="Glyco_hydro_127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14200">
        <property type="entry name" value="RicinB_lectin_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666423" description="Ricin B-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="38"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="2">
        <location>
            <begin position="636"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="776" mass="83447" checksum="986BFB4BC391AC0B" modified="2014-01-22" version="1">MSPSPLNRRQLLQIAGAGAMASAVGSTADFLTNPAAAAVLPPARADIGVSAYPFDLGQVRLTAGRFQDNENRTLNYLRFVDVNRLLYVFRANHRLSTNGAAANGGWDAPNFPFRSHMQGHFLTAWAQAYAVLGDTTCRDKANTMVAELAKCQANNGAAGFNTGYLSGFPESDISAVEAGTPVAVSYYCIHKTLAGLLDVWRLIGNTQAKDVLLNFAGWVDWRTGRLSYNQMQVVLQTEFGGMNEVLTDIHQMTGDSRWLTVAKRFDHASVFDPLAANQDQLNGKHANTLLPKVVGAAREYKATGTTRYRDIAGNAWNMVTGAHTYAIGGNSQAEHFKAPNAIAGYLTNDTCEHCNTYNMLKLTRELWLLDPNRADYFDYYERALLNHLIGAQNPADSHGHITYFTPLKPGGRRGVGPAWGGGTWSTDYNSFWCCQGTGIETNTKLVDSIYFYAGTTLTVNLYVPSVLSWSQRGITVTQSTTYPVSDTTTLTLSGTMSGSWGIRLRIPSWTANPVISVNGAVQNVSATPGSYATVTRTWAAGDTITVRLPMRVVMKAANDNAAVQAITYGPAVLCGNYGNTALSALPSLTVSSIARTSTSSLAFTATANGTTVNLGPFHDAQNFNYTVYWNTGGTSTSGTYKLVNVGTGLVVGVQNMSTADGGLAVGWGDTGTADHNWVRITDGNAVRFRNVNSGKVLGVENMSTADNARVLQWGDTGTADHRWTLVANSDGSYRIRNVNSGKVLAILNASSTWGAQVVQESDNGSADNNWRLVQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8F9</accession>
    <name>U5W8F9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560548.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29750"/>
    <dbReference type="EnsemblBacteria" id="AGZ44211">
        <property type="protein sequence ID" value="AGZ44211"/>
        <property type="gene ID" value="AFR_29750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6027"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142891_1_0_11"/>
    <dbReference type="OrthoDB" id="2021387at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="144" mass="16068" checksum="A6DA1821894429FD" modified="2014-01-22" version="1">MTTPDSPAFEALMAQVMTTAEQFGHREHVHLTWLAVRHYGVSEAIDLISDGIQRTARYAGAPQKYHATVSRAWVELIGHHASETRQEDFAGFADQHPALLDKRLLLRFYRSATLASVQAKQGWVPPDLVPFPWQSLDAHGPGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Q2</accession>
    <name>U5W8Q2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="4 8">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fmt</name>
        <name evidence="11" type="ORF">AFR_30550</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-tRNA(fMet)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="4 8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560707.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30550"/>
    <dbReference type="EnsemblBacteria" id="AGZ44370">
        <property type="protein sequence ID" value="AGZ44370"/>
        <property type="gene ID" value="AFR_30550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6185"/>
    <dbReference type="eggNOG" id="COG0223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033347_1_0_11"/>
    <dbReference type="OrthoDB" id="2009156at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08704">
        <property type="entry name" value="Met_tRNA_FMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00182">
        <property type="entry name" value="Formyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044135">
        <property type="entry name" value="Met-tRNA-FMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="9">
        <location>
            <begin position="2"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl_trans_C" evidence="10">
        <location>
            <begin position="203"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Tetrahydrofolate (THF) binding" evidence="8">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ44370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="308" mass="32609" checksum="9A1291AFD36BD012" modified="2014-01-22" version="1">MRLVFAGTPEVTLPSLDALAASSHEIVAVVTRPDAPAGRGRRLVRSPAGAWADERGIEVLTPQRPREPEFQERLRQIAPDCVPVVAYGALVPPAALEIPKHGWVNLHFSLLPAWRGAAPVQHAVLHGDEVTGASVFELEAGMDTGPVYGTLTEQIRPADTSGDLLARLATEGAGLLVAVLDAIEAGTARAHPQPADGVSLAPKISVEDARVRWNDPAFAVDRRIRACTPAPGAWTTLRDERLKLGPVRPVANAAALKPGEVLVERSQVLVGTATTPVILGEVRAAGKKPMPASDWARGLRLNPGETLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8U8</accession>
    <name>U5W8U8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative permease, DMT superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_36760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36760"/>
    <dbReference type="EnsemblBacteria" id="AGZ45608">
        <property type="protein sequence ID" value="AGZ45608"/>
        <property type="gene ID" value="AFR_36760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7436"/>
    <dbReference type="eggNOG" id="COG5006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057295_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="134"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="30351" checksum="893F311B65E6DBA6" modified="2014-01-22" version="1">MVLGSCASLQIGAALAAHLFPATGSAGATLLRLGLAAIVLLLIARPAVRAWRRDQWREVVLLGVSLAAMNGFFYAAIARIPLGIAVTIEFLGPLTLAAVLSRRGRDFLWVALAALGVLALGWTGPDAAGLDPVGVLYVLIAAIFWALYILAGTRAGAAVPGHGGLAVAMTVAALLVLPGGLSGAATAFTSPSLLLLALGTGLLASVIPYTLEMAALRRLPQAVFGILLSLEPAVAALAGWLLLSQPLSLTTAAAIAVVVLASIGSTLSAARRRPTQPLPLEASAGQHLPDDGLPIPSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W914</accession>
    <name>U5W914_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561950.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36790"/>
    <dbReference type="EnsemblBacteria" id="AGZ45614">
        <property type="protein sequence ID" value="AGZ45614"/>
        <property type="gene ID" value="AFR_36790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7442"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054927_2_1_11"/>
    <dbReference type="OrthoDB" id="848990at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF58" evidence="1">
        <location>
            <begin position="43"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="321" mass="35004" checksum="DC843F97F699535A" modified="2014-01-22" version="1">MNEGASINELAPERRLRRLELMVTRRLDGMLHGSYLGLLPGIGSEPAGSREYRPGEDEVRRMDWSVTARTAVAHVRQVDADRELASWILVDASASMDFGTAEFEKRELAVASVAAVGFLTAGHGNRLGAHLLGPGGVRRFPARSGRTHLLGMLRTLMAVPRALPATDPVPLAEAIDGLHRAVHRRGLVVIVSDFLDGLDDDGLGPGAPDWEQPMRRLAARHQVLAVQISDPREQELPDVGLLTVVDPETGRRREVPTASRKLRERYAAAAATQQASIAGALRRTGAGHLPLRTDRDWVADIVRHVHAQRRLGRASRTGARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W921</accession>
    <name>U5W921_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">D-ser_dehydrat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_30880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DSD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560771.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30880"/>
    <dbReference type="EnsemblBacteria" id="AGZ44436">
        <property type="protein sequence ID" value="AGZ44436"/>
        <property type="gene ID" value="AFR_30880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6251"/>
    <dbReference type="eggNOG" id="COG3616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031639_3_0_11"/>
    <dbReference type="OrthoDB" id="1512472at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-ser_dehydrat" evidence="2">
        <location>
            <begin position="294"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="388" mass="40947" checksum="D0AD055B0F6F65A9" modified="2014-01-22" version="1">MTLDIPDELIDWRAKGFWLPSGEVTTRDFAAAGHTLFDGSFTWPLLVVRDSAVRANIATMAAYADRHGFSFAPHAKTTMAPTLLDAQLAAGAWAMTVATASQALVLRRLGVPRVLIANEVLDATALRWLAAESARGWEVFLQVDSVAGVEAVAAAGGLPVLVELGHAGGRTGARTVEEVLTVARAAAAAPGVSLVGVTGYEGALPDAAAVDEFLATLVASARAMADLLPAEVLVSAGGSAWFDRVIKAIEGQWLDGHQVRAVLRSGASVTHDDGFYRERTPFNREPGEGGLASALEMWAQVLSTPEPGLAIVGMGKRDAPFDEGLPVAEGFTVTKLNDHHTYLSGGAPAVGDLVRFGLSHPCTAFDKWRFIPVADDDDVVVDVLHTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W948</accession>
    <name>U5W948_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562053.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37320"/>
    <dbReference type="EnsemblBacteria" id="AGZ45718">
        <property type="protein sequence ID" value="AGZ45718"/>
        <property type="gene ID" value="AFR_37320"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37320"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7550"/>
    <dbReference type="eggNOG" id="ENOG50331I5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122300_1_0_11"/>
    <dbReference type="OrthoDB" id="2048416at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019675">
        <property type="entry name" value="DUF2550"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10739">
        <property type="entry name" value="DUF2550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="17088" checksum="535D4B838626B524" modified="2014-01-22" version="1">MRVLGIFGICVAALLVVLLAIFLRRRLLMAGGGTIRLQVRVSTMVPGRGWSSGIGQFVGDELRFHRMFSFGIRPKRVLNRRTLEVGRRRLPEGPERLTMPGHWVILLCTTGRAQLEIAMAESTVTGFLSWLEAGPPGQPGSVAPRPTITPRPADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9A5</accession>
    <name>U5W9A5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_37670</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562123.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37670"/>
    <dbReference type="EnsemblBacteria" id="AGZ45788">
        <property type="protein sequence ID" value="AGZ45788"/>
        <property type="gene ID" value="AFR_37670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7618"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_2_3_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041347">
        <property type="entry name" value="MftR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17754">
        <property type="entry name" value="TetR_C_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="208" mass="22261" checksum="43F976F2A38FDF41" modified="2014-01-22" version="1">MQTSPPRSSSGRRDRKKQQTKDALIAAALRLVDERGLERVTVEEISAVVDVSPRTFFNYFTGKDEALIGDPLVDAEEFRARLAALPAEIPVIGALLLALAPAVAQIQADRELWLIRLRVIEQNPGLLPALMARGAVAEREFVAAVAERAGLTPQDAYPQVVAAATGAAFRVAMIRWAAGDGVPPLSDCVHEAFGVLATGLADPTNEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9F8</accession>
    <name>U5W9F8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Alpha-ketoglutarate decarboxylase</fullName>
            <ecNumber evidence="15">4.1.1.71</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">kgd</name>
        <name evidence="15" type="ORF">AFR_37970</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA = (R)-N(6)-(S(8)-succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + CoA</text>
            <dbReference type="Rhea" id="RHEA:15213"/>
            <dbReference type="Rhea" id="RHEA-COMP:10581"/>
            <dbReference type="Rhea" id="RHEA-COMP:10582"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="2.3.1.61"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue succinyltransferase] + 2-oxoglutarate + H(+) = (R)-N(6)-(S(8)-succinyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue succinyltransferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10483"/>
            <dbReference type="Rhea" id="RHEA-COMP:10484"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; tricarboxylic acid cycle; succinyl-CoA from 2-oxoglutarate (dehydrogenase route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.71" evidence="15"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562183.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37970"/>
    <dbReference type="EnsemblBacteria" id="AGZ45848">
        <property type="protein sequence ID" value="AGZ45848"/>
        <property type="gene ID" value="AFR_37970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7679"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0567">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004709_1_0_11"/>
    <dbReference type="OrthoDB" id="29166at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00997"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="PTHR23152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Transket_pyr" evidence="14">
        <location>
            <begin position="902"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="75"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="835"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="91"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ45848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1247" mass="136621" checksum="90486A24CDE509D1" modified="2014-01-22" version="1">MSTQQTSHDNPLADFGPNEWIVDEMYQRYLADPTSVDPAWHDFFADYKPAMASGSIATPDEATAANAKATAAKAGTDAPAASTIAPEKPTVPAAPEKPAAPPAAAPAPAPAKPAPKPAPAAAKTPAPAEGSKVTTLRGVAARIVQNMDASLEVPTATSVRAVPAKLLVDNRIVINNHLARGRGGKVSFTHLIGWALIRSVMAHPEMNNSFAEVDGKPALVQPEHINLGIAIDLAKKDGSRALVVPSIKNCEKMDFRKFWQAYEDVVRRARRNELTMDDYAGTTISLTNPGGIGTVHSIPRLMAGQSAIIGVGAMEYPAPYSGMSDETLTEHGVSKVTTLTSTYDHRVIQGAQSGEFLKAMHEFLLGEHDFYDDIFTSLRIPYEPVRWVRDVARTSEGQIDKAARVVELIHAYRVRGHLMADTDPLEFEIRRHPDLDVLQHGLTLWDLDRLFPVGGFAGKQKMKLRDVLGVLRDTYCRRVGIEYMHIQGPEERRWIQDRIEIKYTKPSPEEQKHVLNRLNAAEAFETFLQTKFVGQKRFSLEGGESLIPLLDEVLQASAEGGLDEMVIGMAHRGRLNVLANIVGKPYEKIFNEFEGQMDPKSAHGSGDVKYHLGQTGKYTAPDEEHSTTVSVVANPSHLEAVDPVLEGIVRAKQDRLDLGLHGYTVLPVLIHGDAAFAGQGVVAETLNLSQLRGYRTGGTVHVVVNNQVGFTTAPEYSRSSLYSTDVARMIQAPIFHVNGDDPEAVVRVARLAFEYRQAFNKDVVIDLVCYRRRGHNEGDDPSMTNPRMYQIIDTKRSVRKLYTEELIGRGDLTVDDAQEQLRDYQSQLEKVFKATRDTAGSAPRPRRNLADEPEPQVDTAVDAAGVRAVGQAHLDLPEGFTPHKRVQQLLDRRAKMSADGGIDWGMGELIAFGSLLAQGVTVRLAGQDSRRGTFTSRHAAIVDSKTGNDFLPLSTLATGNARFFAHDSLLSEYAAMGFEYGYSVENPEALVLWEAQFGDFVNGAQSIVDEFISSGEVKWGQMSSLVLLLPHGQEGQGPDHSSGRPERFLQLCAQDNMRVANPTTPANYFHLLRRQALSSKRKPLIVFSPKSLLRHKLAVSSVADFTEGTFQPIIGDAGVNGTPLDAGSVKRVLLCSGKVYYDLFQARAERGITDTAIIRMEQIYPLPVEELKAVLNQFPNAEDFAWVQEEPANQGAWSFVALNLLEHLEGVRLRRISRPAAAAPAVGSAKMHDAEQSALVEAALPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9U7</accession>
    <name>U5W9U7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phenazine biosynthesis protein phzB 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561023.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32165"/>
    <dbReference type="EnsemblBacteria" id="AGZ44691">
        <property type="protein sequence ID" value="AGZ44691"/>
        <property type="gene ID" value="AFR_32165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6512"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124277_2_0_11"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15558" checksum="169E50012F06D21A" modified="2014-01-22" version="1">MSGTAREIVERLLRAGREVDIDTFVGLMAPEGYLEWPYRPPGVPARVEGRTAIRRHLTEAAEAFITIDDHRNVVIHESLDPEVVIVEYDAHGRVVRTGAPFEQTVIAVFRVRDGQVLSCRDYLNPLPLLEALAGAQGART</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9X9</accession>
    <name>U5W9X9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561123.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32635"/>
    <dbReference type="EnsemblBacteria" id="AGZ44785">
        <property type="protein sequence ID" value="AGZ44785"/>
        <property type="gene ID" value="AFR_32635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6604"/>
    <dbReference type="eggNOG" id="ENOG50342Y9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070789_0_0_11"/>
    <dbReference type="OrthoDB" id="510345at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="306" mass="31943" checksum="E21E0D5046AECD09" modified="2014-01-22" version="1">MTADSVADVKREILVRHLEAWTPAALHRARRATYAHGYADAAPELAEAALRVFTEQSDLVRGRELTMVAVGQETADVARRLAAIQQGAEAAAGLTVHTVGGGTDGALAIALKAAGSARVPLLGFLDATAAGGPPAVTAVSALAAGKPAEAIIVLPAEGFAADDYRAELRAAGFPLVAAVELAVDEEPGEVLVFATASGKSLEGFKDTLWAVDEYAGVRYRDPGDPERHLIDISLNPHPGPLRRELLGHLAEVGESTVTELRTFALTETVYRAEDATRVLHALLTAGSVTRRPEQGRLGGDVVITAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAE1</accession>
    <name>U5WAE1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulatory protein degU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33510</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561297.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33510"/>
    <dbReference type="EnsemblBacteria" id="AGZ44960">
        <property type="protein sequence ID" value="AGZ44960"/>
        <property type="gene ID" value="AFR_33510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33510"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6782"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="138"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="205" mass="21469" checksum="280C8A05C0E4B2C1" modified="2014-01-22" version="1">MIRLILVDDHPVVRHGLRGMLEAEPDLAVVGEASSGPEGVAVAAELRPDIVLMDLRMPGGDGVEATARILATVPGVRVLVLTTYETDRDILRAIEAGAGGYLLKDASPAELADAVRAAARGETVLSPSVASTLVRQVRSPAPPALSARETEVLRLVGQGLTNADIGRALFITEATVKTHLLRAFTKLDVADRTAAVTRAMGLGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBB6</accession>
    <name>U5WBB6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561606.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35070"/>
    <dbReference type="EnsemblBacteria" id="AGZ45270">
        <property type="protein sequence ID" value="AGZ45270"/>
        <property type="gene ID" value="AFR_35070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7096"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_34_6_11"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="158" mass="17324" checksum="9C747254D0BBBDB7" modified="2014-01-22" version="1">MAQVIFRIATRADVPAILDLLTNDEIARSRAGGGSPAATSEEVDAAVWAAFEQIDADPRNELIVADEDGEVVGTCQLTFTPSLSRGGTDRMTIEAVRVRTDRRGRGVGRAMMLWSLGRARERGCRLAQLTTDKRRTDAHRFYSALGFEPSHEGFKMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBJ7</accession>
    <name>U5WBJ7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pseudouridine-5'-phosphate glycosidase</fullName>
            <shortName evidence="6">PsiMP glycosidase</shortName>
            <ecNumber evidence="6">4.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">psuG</name>
        <name evidence="7" type="ORF">AFR_35350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible cleavage of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. Functions biologically in the cleavage direction, as part of a pseudouridine degradation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-ribose 5-phosphate + uracil = H2O + psi-UMP</text>
            <dbReference type="Rhea" id="RHEA:18337"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:58380"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="4.2.1.70"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the pseudouridine-5'-phosphate glycosidase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.70" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561662.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35350"/>
    <dbReference type="EnsemblBacteria" id="AGZ45326">
        <property type="protein sequence ID" value="AGZ45326"/>
        <property type="gene ID" value="AFR_35350"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35350"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7153"/>
    <dbReference type="eggNOG" id="COG2313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012201_0_1_11"/>
    <dbReference type="OrthoDB" id="1294333at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046113">
        <property type="term" value="P:nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1790.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01876">
        <property type="entry name" value="PsiMP_glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022830">
        <property type="entry name" value="Indigdn_synthA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007342">
        <property type="entry name" value="PsuG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="PTHR42909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04227">
        <property type="entry name" value="Indigoidine_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110581">
        <property type="entry name" value="SSF110581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="141"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="315" mass="32243" checksum="EEA08F77FF76AD1A" modified="2014-01-22" version="1">MGRFTIRYGDHVTRARRDGRPVVALESTIVSHGLPRPDNLRVAREIEQTVRDNGAVPATIGMIGGQVVVGLEDPQIEHLAGADGVAKLSVRDLAVAAATGADGATTVAATSAIAAAAGIGVFATGGLGGVHREANTTFDESNDLTTLARTPIVVVCAGVKSILDVGATLERLETLGVSVAGYGTYRFPGFFVTDGGFDVDWKLDSPAQVADVIAARADQGVGAGALVLGNPLPADEQLDPELHDRTLAEGLELLARDGITGKAVTPFLLAHFHGSTQGKSLEVNVRIILRNAALAAQIAAAATPAPAALGFALPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBV0</accession>
    <name>U5WBV0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF397 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562999.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42070"/>
    <dbReference type="EnsemblBacteria" id="AGZ46668">
        <property type="protein sequence ID" value="AGZ46668"/>
        <property type="gene ID" value="AFR_42070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8513"/>
    <dbReference type="eggNOG" id="ENOG503315W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131550_1_0_11"/>
    <dbReference type="OrthoDB" id="1903180at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007278">
        <property type="entry name" value="DUF397"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04149">
        <property type="entry name" value="DUF397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF397" evidence="1">
        <location>
            <begin position="17"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="78" mass="8153" checksum="69E391C55ACF6CCC" modified="2014-01-22" version="1">MTRIVNGMPAGQLEGVTWQKSGRSNPSGNCVECAALPDGSVAMRNSRDPEGAALIYTPAEIEAFLGGVRDGDFDNLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBW2</accession>
    <name>U5WBW2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562935.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41750"/>
    <dbReference type="EnsemblBacteria" id="AGZ46604">
        <property type="protein sequence ID" value="AGZ46604"/>
        <property type="gene ID" value="AFR_41750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41750"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8449"/>
    <dbReference type="eggNOG" id="ENOG503429N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1150826_0_0_11"/>
    <dbReference type="OrthoDB" id="1635506at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666286" evidence="2">
        <location>
            <begin position="30"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="27189" checksum="7935F577660A03DC" modified="2014-01-22" version="1">MRSRPATRVLPALAAAVLTAGLAACGGSAPEEQPPATPSPSAPAAPDEDARVSLAARAAAAEDHRFAALYTLEVPGTEPRSVLATVAVDGSWRVDIPSGALGGTADVSIAQTAAGVFQCAIPSATNPVTPSCVRVADKGKRVPKKFDPKIERVFRQWLPVFTDRQSALSVTAAEPLKGSSGDCYSVDTISASLSAPVDVGIYCYSPEGLLTAARVKFGTLTIASPPAAAPPTIDLPGPVTGGEPMGMSAPPPPPVLPDPSTPVAPVPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCD6</accession>
    <name>U5WCD6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_41890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562963.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41890"/>
    <dbReference type="EnsemblBacteria" id="AGZ46632">
        <property type="protein sequence ID" value="AGZ46632"/>
        <property type="gene ID" value="AFR_41890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8476"/>
    <dbReference type="eggNOG" id="ENOG50331J9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125819_0_0_11"/>
    <dbReference type="OrthoDB" id="1728209at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023869">
        <property type="entry name" value="tRNA_Adeno_NH3ase_assoc_put"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03941">
        <property type="entry name" value="tRNA_deam_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="174" mass="18631" checksum="808FECFB27D990C9" modified="2014-01-22" version="1">MSTFAAAVARGKNGWTASELELTGAADLDEVVDRLRDAEPDADLSLLFVEADDQYLVILRLDEGEDLRVFGSDSAFADESRFGALLLADIETPALEIDDLAAPRPAADDDEDDRPAADPDADPIGDVEILTDLGVSSHRLLTLCGLEGMLPADVTAEICQKLGCGDELEELREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCD8</accession>
    <name>U5WCD8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42850"/>
    <dbReference type="EnsemblBacteria" id="AGZ46824">
        <property type="protein sequence ID" value="AGZ46824"/>
        <property type="gene ID" value="AFR_42850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8668"/>
    <dbReference type="eggNOG" id="COG1971">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_459130_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="599" mass="65250" checksum="700D73680B85AAD6" modified="2014-01-22" version="1">MPSTRPPGAPPDPWTAFAETSERAPGRIRRATRATGRGLIHEYALVVYGSLLLAVLLTWPTLRYPLHTFPQDNWDPSRQAWQVSWAGHILITDPARLWQSNAFFPEAYSFAFGNSLLGYAPAGMIGEGPLAAVLRYNILFVLAHALLAIGGYALIRQLGAGRTGALVGAVAFAYAPWRLAQEGHLDIISAGGIPLALAMLARGHGWSMRYGFRPDRRHAGWAATGWIIATWQVSLGFSLGLPFAYVLGAIFVVVAAAVLVRRLRRRSPRPVLGWRLLVTDGLGLLIFAGVGAMIALPYLRVPDTGPAGTEIKFFSPPLRSLLIGPAESRIWGAPHAVPRESLGWPAEMALLPGFVLYAFALVGLVFSVWRIWQRLILLAAVILGVLLTLGTTFFDGRWTYLPLFGHLPASFGVRIPGRLMVWVTLLLAILAAGAVAEFVRRAEHLAAHRMPPWPGPWLRLATLIPLLLVLVETWNATAHPVVPDQPASMRTVTGPMLVLPTAGLTDQTVMLWSTSRFQQVANGSGGFAPQRQSELRRSVAAFPDAASVQYLRALGITTVLLLRSEVTGTPWERAGDVPVDALGIRREDLDDNSVVFRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCG5</accession>
    <name>U5WCG5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal RNA small subunit methyltransferase G</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">16S rRNA 7-methylguanosine methyltransferase</fullName>
            <shortName evidence="2">16S rRNA m7G methyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gidB</name>
        <name evidence="2" type="synonym">rsmG</name>
        <name evidence="4" type="ORF">AFR_42975</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically methylates the N7 position of a guanine in 16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42975"/>
    <dbReference type="EnsemblBacteria" id="AGZ46849">
        <property type="protein sequence ID" value="AGZ46849"/>
        <property type="gene ID" value="AFR_42975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8696"/>
    <dbReference type="eggNOG" id="COG0357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065341_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070043">
        <property type="term" value="F:rRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00074">
        <property type="entry name" value="16SrRNA_methyltr_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003682">
        <property type="entry name" value="rRNA_ssu_MeTfrase_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31760">
        <property type="entry name" value="PTHR31760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02527">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00138">
        <property type="entry name" value="rsmG_gidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="2">
        <location>
            <begin position="111"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="202"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="236" mass="24201" checksum="DFDB35260CAE7DF0" modified="2014-01-22" version="1">MFGDRLALAARYAELLVTDGVVRGLIGPREAPRVWERHLINCGVMSEMIPIGASVTDVGSGAGLPGIVLAVARPDLTITLVEPLARRTAFLSEAVTALGLDTTVTVVRGRAEDLVGGPPAAADVVTARAVAPLDRLAGWCLPLAAVGGRLLALKGASAAEEVAEHRDAIARLGGADPVVRLCGAGLIDPPTTVVEIVRERAVVAPRPRPSGRAADPGRSTGSAGGRGSGKRRSRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD78</accession>
    <name>U5WD78_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MMPL domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_38260</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562241.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38260"/>
    <dbReference type="EnsemblBacteria" id="AGZ45906">
        <property type="protein sequence ID" value="AGZ45906"/>
        <property type="gene ID" value="AFR_38260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7740"/>
    <dbReference type="eggNOG" id="COG2409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005108_1_1_11"/>
    <dbReference type="OrthoDB" id="149255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="521"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="582"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="638"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="668"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="193"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="730" mass="75064" checksum="C62ABD37415E7121" modified="2014-01-22" version="1">MAQLLYRLGRFSFRRRRLVAGLWVLVLVLLVVGALTLGGKTVDTFSIPGTESQEALDALKRDLPDAGGASLTVVVEAPKGQALTDAVTKTAVADVVTQATKLPGVVAVFDPYQSGRIDRTGTVGLISVQYAKGDDELTSADREAFDGLAADGTPQRPRVVPGGVSGGPPEIGASEVIGVAVAALVLVVTFGSLVAAGMTLLTALFGVIAGMCGLLLVTAVVDVSSTAPILALMLGLAVGIDYALFISSRHRTQLAAGLPAEESVARATATAGSAVLFAGATVVIALAGLSIVGVPFLTAMGLAAAGTVLTAVLVALTLLPAMLGFTGTRILPRGLRSRGAHARKTEDAGFGFRWARWVIRLRVPVLIVVVAGLGALSVPTREMRLALPDNGTAPAGSHQRIAYDLTTAAFGAGANGPLLVVIRGPRGDTVSSLAGRVTAGVRKLGDVAAVGPGPANAAGTTRVIMVTPNSGPSSEETAQLVHDLRDSVELLAGGTTSVAVTGATAVAVDVSQKLTSALPRYLFVVIGLSFLLLLLAFRSVLVPLKATAGFLLTMGATFGITVGVFQLGWAADVFGVDRPGPLVSFLPIIMMGVLFGLAMDYEVFIVSRVREDFVHGGPPTDATAAAATVAGVGHGARVVTAAALIMAAVFGGFVLIEDPTIKTIGFGLAIGVLIDAFVVRMTLVPAVLSLLGRRAWAFPRWLDRITPRVDIEGESLDRQREAETPELVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDA9</accession>
    <name>U5WDA9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oligopeptide-binding lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0301 (AlgH) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562271.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38410"/>
    <dbReference type="EnsemblBacteria" id="AGZ45936">
        <property type="protein sequence ID" value="AGZ45936"/>
        <property type="gene ID" value="AFR_38410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7770"/>
    <dbReference type="eggNOG" id="COG1678">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051842_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003774">
        <property type="entry name" value="UPF0301"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02622">
        <property type="entry name" value="DUF179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="SSF52129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="423" mass="46118" checksum="AC99557ABB1BCF58" modified="2014-01-22" version="1">MSEVADPAASQVVLIGVSEYRRLFTLPAARRNVTDLRDLLQHEDLWGVPPGNCHVLLDPESPAEVSRAIRKAASATGSDGLLLVYFAGHGLVDADDDNLILGLPGCDPEVPYERGVPYDWIRRAVADTRARRRVVILDCCYSGRAGSELGGDGTGTDFIADKAEAEGTCLLVSAPANRPAMAPLGEAYTAFTGELIRLLRDGLRPPPPETIPATLTVHTLWRAVRRAMLRRGFERPELRARDAGGEIALVHNPAAPRHNLAGSVLYAAPWFVDEDLGQRAILVLRHNQTGALGVCITRPDGDIPDEFPEAWRRLLRNPAMLFHGGPVARDGYIVVTLLRSGAPEPLRFTPVRDRLGTMALSASPEDVSGTVDAMRVFVGYLGWRAGELEEFLDRGALIASRHTARQVFTERPGELWQSLQAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDI3</accession>
    <name>U5WDI3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38785"/>
    <dbReference type="MEROPS" id="S09.073"/>
    <dbReference type="EnsemblBacteria" id="AGZ46011">
        <property type="protein sequence ID" value="AGZ46011"/>
        <property type="gene ID" value="AFR_38785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7848"/>
    <dbReference type="eggNOG" id="COG1506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006105_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S9" evidence="1">
        <location>
            <begin position="435"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="585" mass="62440" checksum="0D2038FF574E97FA" modified="2014-01-22" version="1">MLVGVEYPELADRTGRFRFGAPHALTVGDDGARVAFLRSAGPRDPAAALWVLDVATGALSQVAAGPVPAYAGSANLDVLAWAHDGRLFSSVAGHIATAAEVLDPRPDPAGQHIGYVTPDGSLRVVSGSDDQLLAGEAGSGVSWGVPEPVAAEFGRDRGWWWSPDGSQILAVRSTGTALSLHLLDLHGGWVDVHWDRETYPYLVGVRWSGTGNALITVLRRTQQHGLVLSVDPRTGETQVHAELADARWVEPVPGTPRQLPDGRVLVGGELAHDGFDARCLFADGSLITPPGLYVRRVAGVLRRPTGGPELIIEGTDGEPSEQHVFAVRTALGGGTASARRLTTEPGWHTATLGGDVLILDLTVTRGAAKVCTLPSLAEPLVYHPEPVLERVTDRRLPTGVLYPTGHFDGTKLPVLLELGDGPGHQQVVSDPARWQEKQWWADAGFLVVTVDTRGTPGVAPSFEKVIYRRLADVALTDQIDALHALTGKHPDLDLTRVAVRGTGLGGWLAAAAVQRRGDLFRCGIARDPVTDWSDLPPAYAERYLGSRADNSDIYEHHRVDASAPTLLISPAGGLSEELTYVTRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDQ7</accession>
    <name>U5WDQ7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_39505</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562489.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39505"/>
    <dbReference type="EnsemblBacteria" id="AGZ46155">
        <property type="protein sequence ID" value="AGZ46155"/>
        <property type="gene ID" value="AFR_39505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7992"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_2_11"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="4">
        <location>
            <begin position="33"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="475" mass="50852" checksum="0E3F62AD1CA67402" modified="2014-01-22" version="1">MFEYAPAPESRSVVDIAPSYGLFINGEFVPASSSGGVFKTVNPASEEVLAEVASAGAEDVDLAVAAARRAFGPWSALPGAERAKYLFRIARIIQERSRELAVLESLDNGKPIKESRDVDLPLVAAYFFYYAGWADKLAYAGFGANPQPLGVAAQVIPWNFPLMMLAWKIAPALAAGNTVVLKPAETTPLSALFFADICRQADLPPGVVNIITGAGETGRTLVEHPDVNKVAFTGSTEVGRQIARSVAGTRKRLTLELGGKAANIVFDDAPIDQAVEGIVNGIFFNQGHVCCAGSRLLVQESAYDEVLTALKRRMATLRVGDPLDKNTDIGAINSAAQLARIRELSDIGASEGAERWSPPCELPSQGFWFAPTIFTGVTQAHRIAREEIFGPVLSVLTFRTPDEAIEKANNTPYGLSAGVWTEKGSLILAMADKLRAGVVWANTFNKFDPTSPFGGYKESGYGREGGRHGLEAYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE60</accession>
    <name>U5WE60_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_39785</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562544.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39785"/>
    <dbReference type="EnsemblBacteria" id="AGZ46211">
        <property type="protein sequence ID" value="AGZ46211"/>
        <property type="gene ID" value="AFR_39785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8049"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_11"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="5"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="292"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="503"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="532"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="324"/>
            <end position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="601" mass="65336" checksum="0AA2B848D9F71D21" modified="2014-01-22" version="1">MANIINLDRVNKGYGAAGQLLTDVSLGLDDDARVGIVGLNGAGKSTLLRMLAKLEEPDSGRVTHRRDLRVAALPQTLTLAPEATVRDVVLGTAWLSEGMGAEHEWAGDAGVRTILDGLGMGHLGLETPVGPMSGGERRRVALAALLVRESDLLILDEPTNHLDVAGVDWLARYLLGRRGALVVVTHDRWFLDAVCTATWEVVDEQVYAYEGGYAAWTLSRAERLRVAAAVESRRQNLLRKEIAWLRRGPPARTSKPQFRIDAANALIADEPPVRDQVSLQRLATTRLGKQVYDLEDVTLHAGPKPIFANLTFQVGPGDRIAILGANGAGKTTLLRMLAGVTRPDGGRLVTGSTVRAAFLSQELKELPGHMRLLEAVEDVARRVKLGDRELSAGQLAEVFGFTDKRIWTPVSDLSGGERRRLQLLRLLATEPNVLLLDEPTNDLDTDTLASLEDLLDSWPGTMVVASHDRYLVERVTDTAYGMFGDGRLVHLPGGIDEYLSRVGNPAAPPSAHTAGGKAPAAPTADGMSAAELRVARKDLSRLERQVAKLDERILKINESLAVHGSDYDKILELDAQLKAVQEERGQVEEAWLELAERLPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEA1</accession>
    <name>U5WEA1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoacyl-ACP reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562589.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40010"/>
    <dbReference type="EnsemblBacteria" id="AGZ46256">
        <property type="protein sequence ID" value="AGZ46256"/>
        <property type="gene ID" value="AFR_40010"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40010"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8097"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_11"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="314" mass="32644" checksum="4D1AD3F77C90A789" modified="2014-01-22" version="1">MVFDARSASGSRSRRDATRPAAGRRPRSAAPSSEPEQDQEPDPVDLTQDEPATEVEAEEGPVTLRLDGKVALVTGAGSPDGIGYATARRLRDLGARVAIVSTTRRIHERASELGVTGFVADLTDEAEVGALADAIADQLGDVEVLVNNAGLASRTSPEVLRPVAQLTYDEWRAEIDRNLSTAFLCSRAFVGGMSEQGWGRIVNLAATAGPINALPTEAAYAAAKSGVVGLTRALAMELVADGINVNCVAPGTIYTAASTVTEIKQGLGTPIGRPGTPDEVAAAIAFLCSPAASYITGQMLVVDGGNSVREAEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFK7</accession>
    <name>U5WFK7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563037.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42260"/>
    <dbReference type="EnsemblBacteria" id="AGZ46706">
        <property type="protein sequence ID" value="AGZ46706"/>
        <property type="gene ID" value="AFR_42260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8555"/>
    <dbReference type="eggNOG" id="COG0431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_2_2_11"/>
    <dbReference type="OrthoDB" id="1818437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="170" mass="18291" checksum="C087061B7357A219" modified="2014-01-22" version="1">MAVIVASTREGRFGPTVAAWFAGEARGRSDLEVDVVDLADEETVTGRLGLADAFAVVTPEYNHSYPAPLKAVIDAHHSEWHAKPVGFVSYGGLSGGLRAVEHLRPVFAELHAVTVRDTVSFHGGRYCFGSDGLPTDPSECGAAAGRLLDRLAWWGTTLREARETRPYVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP84</accession>
    <name>U5VP84_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative SNF2-family helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_01615</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01615"/>
    <dbReference type="EnsemblBacteria" id="AGZ38612">
        <property type="protein sequence ID" value="AGZ38612"/>
        <property type="gene ID" value="AFR_01615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.325"/>
    <dbReference type="eggNOG" id="COG0553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_21_8_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022138">
        <property type="entry name" value="Helicase_SWF/SNF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12419">
        <property type="entry name" value="DUF3670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="521"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="811"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ38612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="983" mass="105702" checksum="8E989758F1657493" modified="2014-01-22" version="1">MPSEALAAALTGSSDDLRDILAKAGAAEVTLQLPGTAKGPLPSPETGSEAPGKGLRLGAWETPALVLPAESALEALSALAEPDPDGPWSAAVSLRYLCVLAGQACDLARRGRMLPQLVTESGVPAARWQPVLTGADAASYRDFAAALPPICRAVTTSGGTTDDAPSDAGGSGVGRTLRDALEVLVDSAARQLMPERLMVGIRPGPKAPLPDRFIAALTAPDPALPGARPAEVAELAKPLIGWLRAAHEANGPIRVSFRLAEPPPGEDDWALDFALQSADDPSLYLPAADLWTGARMPGLPSQPDETLLAGLGRAVRLFPALHEALLRAQPDAMTLSTSEAHEFLRQSAPLLQAAGFGVQLPAWAGRKAVGLKLTTRTRSKTPSGKAAAESGFGLDQLVDFRIDLVIGEGTVSAEELAELARLKVPLVRIRGQWVELDDRQLKAALKAVGRRREGQLTAAEVLQQVVDGGEEDLPLVEVDADGSLGDLISGQAAERLTPVPTPPGFQGTLRPYQERGLSWLHFLARLGLGGILADDMGLGKTAQTLSLLLAERLDAETPVAPTLLVCPMSLVSNWQKEAARFAPSLRVHVHHGGGRKRDEDFRDTVAASDLVVTTYGTALRDLGLLREVHWGRIACDEAQAIKNSGTRQAQAVRDIPARTRLALTGTPVENHLAELWSIMEFCNPGLLGPAKRFRRRFQEPIEVRGDADATAALKRATGPFVLRRLKTDKSIISDLPEKNEMKVWCTLTAEQATLYQAVVEDMMAVIDGSEGIQRRGNVIAAMTKLKQVCNHPAHLLKDGSRLPGRSGKLARLEELTEEIIEDGDKALIFTQYAEFGTMLQPYLAAHLDRPVLWLHGGLSKAKRDELVDRFQNDTEPMLFLLSLKAAGTGLNLTAANHVIHVDRWWNPAVENQATDRAFRIGQSRNVQVRKFICTGTLEEKIDAMIERKKELASAVVGTGEDWVTNLDTDQLRELLSLDPVAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPQ9</accession>
    <name>U5VPQ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02500"/>
    <dbReference type="EnsemblBacteria" id="AGZ38789">
        <property type="protein sequence ID" value="AGZ38789"/>
        <property type="gene ID" value="AFR_02500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02500"/>
    <dbReference type="HOGENOM" id="CLU_2393223_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="93" mass="10459" checksum="D0A6994D19DBFB16" modified="2014-01-22" version="1">MPFGLKLPPNSDAQWWRTPPRMEEDMVAKGLKPPPPIIIVNRPSKRVSPAPEKPSRPYGFLPTLGNHLPPIIWTALAPPPMNMPPRKLRARSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPW8</accession>
    <name>U5VPW8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357792.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02800"/>
    <dbReference type="EnsemblBacteria" id="AGZ38849">
        <property type="protein sequence ID" value="AGZ38849"/>
        <property type="gene ID" value="AFR_02800"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02800"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.562"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_2_11"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="155" mass="17569" checksum="E48967582109EEC0" modified="2014-01-22" version="1">MLRPAADADRDTVLAWRNHPDVRAVSLTTHVIAPEEHAKWWDVVLEDPDRHVLIHDDSGVVIFDCREDPPSWSFYLDVEGLGAGLLAGWMRLEKEAVAYAFGELGLTRLGGETLATNKQVLALHRRFGFTEVRRYERLIDGTPQVVVWTERGIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ59</accession>
    <name>U5VQ59_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">L-arabinose isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_03300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357892.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03300"/>
    <dbReference type="EnsemblBacteria" id="AGZ38949">
        <property type="protein sequence ID" value="AGZ38949"/>
        <property type="gene ID" value="AFR_03300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.664"/>
    <dbReference type="eggNOG" id="COG2407">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045786_1_0_11"/>
    <dbReference type="OrthoDB" id="507566at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008733">
        <property type="term" value="F:L-arabinose isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019568">
        <property type="term" value="P:arabinose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038583">
        <property type="entry name" value="AraA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004216">
        <property type="entry name" value="Fuc/Ara_isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009015">
        <property type="entry name" value="Fucose_isomerase_N/cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003762">
        <property type="entry name" value="Lara_isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38464">
        <property type="entry name" value="PTHR38464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02610">
        <property type="entry name" value="Arabinose_Isome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50443">
        <property type="entry name" value="SSF50443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53743">
        <property type="entry name" value="SSF53743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0054">Arabinose catabolism</keyword>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ38949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="473" mass="51820" checksum="52097C4997FB32B4" modified="2014-01-22" version="1">MSDLLPAKQRRKTRIGLVAGGLGTYWPQFPDLLPQLQESARYVSQRFQDMDAEVTDVGFISDAQEGAVAAEKLRQADCDLIVLFLTTYLTASMVLPIAQRANTPVLVIDLQPTEKMDHASFDTGAWLAYCGQCPVPEMGNTFRRAGIPFRSVSGWLRQESAWQRIGQWIRAAHVRAALRHARHGLMGHLYPGMLDVSTDLTLLPVTFGSHVEVLEFDDLRQRVEKVTESETKERMDLARQIFTLDDTVQEEDFAWGATVSVALDRLVDDFELDSIAYYHRGLDGEQHERLGAGMILGASLLTARGIPATGEYELRTTVAQLATQAVGAGGSFCELQALNFEDNVVEMGHDGPAHLAVGARDPLLRGLGVYHGKRGWGVSVEFDVRPGPVTLLGLGQDRDGSLSFIAAEGTVVPGPLLAIGNTTSRVDFGRDPGEWVDEWSASGVGHHWSLSLDHRAADYKAAASLLGIEFRQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQR3</accession>
    <name>U5VQR3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Transcription termination/antitermination protein NusG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nusG</name>
        <name evidence="9" type="ORF">AFR_04425</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Participates in transcription elongation, termination and antitermination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the NusG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358140.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04425"/>
    <dbReference type="EnsemblBacteria" id="AGZ39174">
        <property type="protein sequence ID" value="AGZ39174"/>
        <property type="gene ID" value="AFR_04425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.895"/>
    <dbReference type="eggNOG" id="COG0250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067287_0_1_11"/>
    <dbReference type="OrthoDB" id="2027986at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00948">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
        <property type="entry name" value="NusG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001062">
        <property type="entry name" value="Transcrpt_antiterm_NusG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015869">
        <property type="entry name" value="Transcrpt_antiterm_NusG_bac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
        <property type="entry name" value="PTHR30265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265:SF2">
        <property type="entry name" value="PTHR30265:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02357">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00338">
        <property type="entry name" value="NUSGTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="SSF82679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00922">
        <property type="entry name" value="nusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01014">
        <property type="entry name" value="NUSG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="3 5 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="NGN" evidence="8">
        <location>
            <begin position="69"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="250" mass="27497" checksum="B33DFE85965B7BAE" modified="2014-01-22" version="1">MPEYDDETAPAAEEQSAVATVDDDESVEAASAEPVAEAEAAEAQAAEAPEADENYDPVAELRQKLRYAPGDWYVVHSYAGYENKVKTNLETRITSLDMEDFIFQVEVPTREEVEVKNGKRLQVQNKVFPGYILVRMDLSPESYSCVRNTPGVTGFVGATDRVDRPAPLSLDEVLKWLAPAVEAEEKKAKPEIRVLDFEVGDSVTVTDGAFASLPASISEINADQQKLKVLVSIFGRETPVELNFNQVAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR96</accession>
    <name>U5VR96_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1232 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_05425</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358905.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05425"/>
    <dbReference type="EnsemblBacteria" id="AGZ39374">
        <property type="protein sequence ID" value="AGZ39374"/>
        <property type="gene ID" value="AFR_05425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1099"/>
    <dbReference type="eggNOG" id="COG3339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132031_0_0_11"/>
    <dbReference type="OrthoDB" id="2017850at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010652">
        <property type="entry name" value="DUF1232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06803">
        <property type="entry name" value="DUF1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="DUF1232" evidence="5">
        <location>
            <begin position="53"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ39374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12478" checksum="85FAE927248B139A" modified="2014-01-22" version="1">MPRTLQRTAAFTALAKALVSGSRGGPSIGKRLAALPRMLKATAKGQYDGGLRVAMMAAATAYVVSPIDVIPEAMFLVFGLADDAVMITWLAGTVLAETQRFIEWEKQRDAVLPGSVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRQ4</accession>
    <name>U5VRQ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative response regulator receiver domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_06040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359028.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06040"/>
    <dbReference type="EnsemblBacteria" id="AGZ39497">
        <property type="protein sequence ID" value="AGZ39497"/>
        <property type="gene ID" value="AFR_06040"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06040"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1227"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_11"/>
    <dbReference type="OrthoDB" id="1729688at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="14856" checksum="49D9748A4DC84913" modified="2014-01-22" version="1">MAAVLIAEDDEEIAFILTRVLKRAGHVVHHAPDGVAALALASQIRPQVVLTDLGMPRMDGLELSRSVREHPDLRDTPIAILTGSLLPEDPRAVAAAPCAVLLKPCANDDLRLAVQQLAELGPHDHSTASSACPFRLAALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRT3</accession>
    <name>U5VRT3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative_PNPOx domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357388.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00780"/>
    <dbReference type="EnsemblBacteria" id="AGZ38445">
        <property type="protein sequence ID" value="AGZ38445"/>
        <property type="gene ID" value="AFR_00780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00780"/>
    <dbReference type="eggNOG" id="COG3467">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139738_0_0_11"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019965">
        <property type="entry name" value="PPOX_F420-dep_Rv2061_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03666">
        <property type="entry name" value="Rv2061_F420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="5"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="123" mass="13281" checksum="0FAC02943E0D79ED" modified="2014-01-22" version="1">MPTLEQLGSEKYVLLTTFRRDGRAVATPLWVVPDGTGVAFWTPADTGKVKRIRNSGRVTLAPCDVRGNVKGEAVEATARIGDGADRRRIGATLQRKYGLMGRLTMLGSRLRRGVDGTVAVLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRV8</accession>
    <name>U5VRV8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="6">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="6">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_06375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359095.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06375"/>
    <dbReference type="EnsemblBacteria" id="AGZ39564">
        <property type="protein sequence ID" value="AGZ39564"/>
        <property type="gene ID" value="AFR_06375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1293"/>
    <dbReference type="eggNOG" id="COG0127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082080_0_1_11"/>
    <dbReference type="OrthoDB" id="1824064at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="Ham1p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="PTHR11067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00042">
        <property type="entry name" value="TIGR00042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="9"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="158"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="204" mass="21494" checksum="78ACEA8384D56FC1" modified="2014-01-22" version="1">MSARLLLATANQKKLVELQRILDNALGTGGIELVGLGDFPGYPDVPETGLTFGENALIKAREGASRTGLPTVADDSGLAVDALNGMPGVFSARWSGRHGDDDANLDLVLAQIGDVGDEHRGGAFVCAAALVLPGGREHLVEGKQRGEILRARRGTGGFGYDPIFLGEGQTRTNAELSPAEKDAISHRGKAFRELSKVIAKELPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRZ4</accession>
    <name>U5VRZ4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Winged helix family two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_01105</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357453.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01105"/>
    <dbReference type="EnsemblBacteria" id="AGZ38510">
        <property type="protein sequence ID" value="AGZ38510"/>
        <property type="gene ID" value="AFR_01105"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01105"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.220"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ38510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="25354" checksum="6B86D844F090418E" modified="2014-01-22" version="1">MRVLVVDDDPDVRDSLRRSLSFEGYRVTTAADGVQALDSVAGARPDVVVLDLQMPLLDGIETCRRLREQGNDVPVLMLTARDGTRDRVTGLDAGADDYLAKPFALEELFARLRALLRRPRQPEGELLRFADLELDPATRDASRAGRAITLTRTEYDLLELFLRHPRQVLTRQQILAEVWGYDFDPGSNTLEVYIGYLRRKTEQHNPVRLVHNVRGVGYTLKVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS23</accession>
    <name>U5VS23_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methylamine utilization protein MauE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_06490</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be specifically involved in the processing, transport, and/or maturation of the MADH beta-subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">One-carbon metabolism; methylamine degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359118.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06490"/>
    <dbReference type="EnsemblBacteria" id="AGZ39587">
        <property type="protein sequence ID" value="AGZ39587"/>
        <property type="gene ID" value="AFR_06490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1316"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101331_0_0_11"/>
    <dbReference type="OrthoDB" id="1721242at2"/>
    <dbReference type="UniPathway" id="UPA00895"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030416">
        <property type="term" value="P:methylamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009908">
        <property type="entry name" value="Methylamine_util_MauE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07291">
        <property type="entry name" value="MauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="MauE" evidence="11">
        <location>
            <begin position="14"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ39587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="17076" checksum="7DDC0E92E9B16BB7" modified="2014-01-22" version="1">METGQSAHTTAVWPWISTVARLGLAAVWLVAGGLKVGDLAESGRAVNAYEIFPYDVAKVIGAAQPFLEIALGLLLLVGLAVRLSAAISAGLLLIFIAGITYAWVQGLQIDCGCFSSGGALGAGESPEYTLDIVRDVGFLLLAGILLWKPRTRFSIDGVLMGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSE4</accession>
    <name>U5VSE4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_08145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08145"/>
    <dbReference type="EnsemblBacteria" id="AGZ39918">
        <property type="protein sequence ID" value="AGZ39918"/>
        <property type="gene ID" value="AFR_08145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1662"/>
    <dbReference type="eggNOG" id="COG2018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139202_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="109" mass="11691" checksum="73666F0E62766BFD" modified="2014-01-22" version="1">MGASIVDYTTGFPVATSGAAPNEDHEAAAAGTADVVQAAQSRSLFVSAVPHDLLEDLIITTAGGYHLLRLVQTDFDSRLVLYVWLDRIHGNLAVARRRMQTLADELVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSR0</accession>
    <name>U5VSR0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative beta-N-acetylglucosaminidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08670"/>
    <dbReference type="EnsemblBacteria" id="AGZ40023">
        <property type="protein sequence ID" value="AGZ40023"/>
        <property type="gene ID" value="AFR_08670"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08670"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1764"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008392_5_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_3" evidence="2">
        <location>
            <begin position="64"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="536" mass="54738" checksum="14346FB9C7497EE7" modified="2014-01-22" version="1">MAPSVSVPPSPAGDPALRAREAVKTFTDADLAGQVLMPYAYGDAADRVDKAAAAGNQNLAGVNTPAEMITKFRLGGLILVGFTPDDPTGATNPATNVENPKQVRALTDGLQQAATQLPGAAPLLIGTDQEYGVVTRVKEGVTLLPSAMTLGAAGKPDLTEGAWRAAGGELAAMGINLDFAPVADTLGSQGSSVIGSRSFGADPKANAAQVQAAVRGIQGAGISAALKHFPGHGHTTGDSHDDLPIVRQSLQKWTEEDRPPFGAGINAGAGVVMSGHLDVEALDKGVAATFSRKIMTDLLRTDLKFTGVAITDAMNMPPAMKWPPGEAAVRALNAGNDMLLMPPNLAGARDGILAALKDGTLKRERLVEAVTRVLTLKYRNAAKQQPALDTIGSAAHQKAVYDADAAAITLLKGSCTGPLVTGPVTVTASGGREAARIALVKALQDTGVKVQAAGGTVVHLVGYGDRQTDLSMDAAVTVVMDTPYLLAGSRSPVLLATYSSSPLALTALANVLAGKAKAPGKSPVAVDKLPRSACGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT40</accession>
    <name>U5VT40_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359560.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08700"/>
    <dbReference type="EnsemblBacteria" id="AGZ40029">
        <property type="protein sequence ID" value="AGZ40029"/>
        <property type="gene ID" value="AFR_08700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1770"/>
    <dbReference type="eggNOG" id="COG0331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030558_1_2_11"/>
    <dbReference type="OrthoDB" id="1123553at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="SSF55048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_AT" evidence="2">
        <location>
            <begin position="5"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="319" mass="32200" checksum="299D85D639770853" modified="2014-01-22" version="1">MLAVLSPGQGSQKPGFLSPWLGLPGAEARLRWWSALAGVDLIHLGTEAGADEIKDTARTQPLLVAAALLAAEQLPLHNVDVVAGHSVGELGAAAIAGVVPAEAAIALAGLRGREMAAACALEPTGMSAVLGGDADEVVATIERHGLFPANRNGAGQIVAAGSLDGLAKFAAEPPAKARVITLQVAGAFHTPFMAPAEQALSAVAGGVTVTDPSRVLLSNLDGTAVADGAEMLQRLVRQVTAPVRWDLVMGTLRELGVTGVLELPPAGTLAGLVKRELKGDGAPEIVTLNTPDDLDAARDLIARHGTAAGPAASTSGQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTV0</accession>
    <name>U5VTV0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360040.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10050"/>
    <dbReference type="EnsemblBacteria" id="AGZ40299">
        <property type="protein sequence ID" value="AGZ40299"/>
        <property type="gene ID" value="AFR_10050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2048"/>
    <dbReference type="eggNOG" id="ENOG5032YYX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082043_0_0_11"/>
    <dbReference type="OrthoDB" id="1919338at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="295" mass="30353" checksum="2492C0C98F572449" modified="2014-01-22" version="1">MIVASLLLILVAVTLLVIGLAGGSSSLLISSIVASLLAAVALVIGARQASAARRAGTTSDDAAADPALSRPATVRMADLDDLPEPQSGHETARREEDDDLATPDVTQRVHPDADLHDEAAAGRASSVQPAGAASRDDDPAYDQASTVIGAARADAAEDNAWRREPAPPTSASSSSSVSSSDEAAAAAPAGSTSEPDFNRLQDTGPSEEFDEPDPEDPDDEPLPQAVRPADAVRVARLDAEVLVVDGRPRYHLADCPHLVGRLTEGLPVAEAVELGFSPCGLCRPVDRLVAAAARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU03</accession>
    <name>U5VU03_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">UDP-N-acetylmuramate--L-alanine ligase</fullName>
            <ecNumber evidence="4 11">6.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">UDP-N-acetylmuramoyl-L-alanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">murC</name>
        <name evidence="15" type="ORF">AFR_10015</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine</text>
            <dbReference type="Rhea" id="RHEA:23372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.8" evidence="4 11"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360027.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10015"/>
    <dbReference type="EnsemblBacteria" id="AGZ40292">
        <property type="protein sequence ID" value="AGZ40292"/>
        <property type="gene ID" value="AFR_10015"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10015"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2041"/>
    <dbReference type="eggNOG" id="COG0773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028104_2_2_11"/>
    <dbReference type="OrthoDB" id="307881at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008763">
        <property type="term" value="F:UDP-N-acetylmuramate-L-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00046">
        <property type="entry name" value="MurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005758">
        <property type="entry name" value="UDP-N-AcMur_Ala_ligase_MurC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01082">
        <property type="entry name" value="murC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 11" id="KW-0133">Cell shape</keyword>
    <keyword evidence="10 11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="12">
        <location>
            <begin position="19"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="14">
        <location>
            <begin position="122"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="13">
        <location>
            <begin position="324"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="124"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ40292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="472" mass="49916" checksum="81893E9191080E81" modified="2014-01-22" version="1">MNTAELTPAGELTAEELGTVHLIGIGGVGMGGLARLLLTRGVPVSGSELREWPALAALRALGGTVHMAHEASNLDGVDTVVYSTAIPQDHVEIVEARRRGLRVMHRSEALAAAMTGRRTIAVAGTHGKTTTTSIMTVILQHAGEDPSFVIGGEISAAGSNGHHGSGSYFVAEADESDKSFLIYRPHVSIITNIEGDHLNNWGDLAGLKAGFLEFAQLTDTDGFVVTCIDDEGVGELIAGLRAAGRTVYTYGESEGADLRISDVVSSVQGVRYQATLDGKPLGEIQLALPGRHMGLNSAAAVLTAMRLGLPLQKITEALSSFPGVRRRFERKGTVAGVRVYDEYAYHPTAVKAALRTLREVAGDGRLVVVFQPYRVYRTRDLQSELAEGLAIADEVIVMEVFGPGEQRGPGEGGVALTAAIDLSPEHKVFVPSWEDVAAEVVRRSRPGDVVVTMGAPPISMMGDELLAALAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU28</accession>
    <name>U5VU28_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gamma-glutamyltranspeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_04185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04185"/>
    <dbReference type="MEROPS" id="T03.013"/>
    <dbReference type="EnsemblBacteria" id="AGZ39126">
        <property type="protein sequence ID" value="AGZ39126"/>
        <property type="gene ID" value="AFR_04185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.845"/>
    <dbReference type="eggNOG" id="COG0405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_0_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="449" mass="45488" checksum="CAE0DD4F5F9E035E" modified="2014-01-22" version="1">MQILRAGGSAADAAVAAVLATCVTETIYTGLGGGGFATYFDAATGSVTCLDFFVSVPGTDGDRQAGPMIAIDVFFGGMPQVYSIGGASVAVPGVPAGCGEVHRRWGRLPWADLVAPAIGLARTGAILPAAHARTLEACAPALAYGEGAAIYAPNGKLLQGGDLLFHPGLAAALEGLASEGPGLFYTGAYASLLVDTVRAGGGALGPLDLAAYRVLETPVDHATLGAYRVRARHDLNRTVDTIAALPGNLTDLKRPARAVAVAAALRDRGRQKIGDTTNVSVVDADGNACVITTTMGLGAAVWLPGLGVNLNSMLGEGELITDDLAPGVRMSSNMCPLVVIDAAGDLAVAAGSAGASRIRTALVDTLLGVLVDGLDMPAATSRPRFHVVDDTVHAEPGLPRDELDALADAGWTINEWPEINHYFGGVTAVGVGGAAGDPRRDGVGLLLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUC7</accession>
    <name>U5VUC7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_05205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05205"/>
    <dbReference type="EnsemblBacteria" id="AGZ39330">
        <property type="protein sequence ID" value="AGZ39330"/>
        <property type="gene ID" value="AFR_05205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1053"/>
    <dbReference type="eggNOG" id="COG0489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_475420_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="505"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="514"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="614" mass="64532" checksum="F9DDD3B4CEFD5D99" modified="2014-01-22" version="1">MSADLSHYLGVFRRHWWVVLTAGGVGLGLGALVTQTIPKVYEASTSVLVQAVSGDVNASGGRTKGTINLDTEAQLVGSGAVAVKAATLLRTDVSPIELAHDVSVEVPANTTVLVIRFEADSPKGAQAGSHAFAEAYLRNREETARAGLDQQIKTLTTKVKQLTATLTSINNRLAKAEDGSFERGNLESLRNNSQNQLNSLTGRLNELTTATVGAGSIISDAREPEAPTSPNAMLNIATGGMIGLLIGLVLAFARERFDGRLRTAADVRDRGRIPVLAALDERTTPHFDDVLQPYGPGGRVFNRLRNEVLASLSTGDQIIVVTGASRGSATTLVAANLAAALARTGSDVVLIGAHLPDSVVEAAPLARMLGVSAMPGLSDLLAGRIGLNKAMQRTPRIPSLRVITTGGAATAAGLMQSQRLRDTLEALRRQTGYVVIEAPSTSSSADAQSLASLADAAILAVELRRAKRPALLDAAEQLSRVGTPLLGAVVLPRLTSLRDADENLPAVTLPSERREQLPSERREQLLSERREPRPDETAVLDTERTQPMRISTGPKPGGSGGPGGPVRPRDGGIDDVTAVIDMGSNGPHTKVSPKPKAGTQTQTAEKPGDQPEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUC9</accession>
    <name>U5VUC9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Suf system fes assembly protein, nifu family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_10690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10690"/>
    <dbReference type="EnsemblBacteria" id="AGZ40427">
        <property type="protein sequence ID" value="AGZ40427"/>
        <property type="gene ID" value="AFR_10690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2179"/>
    <dbReference type="eggNOG" id="COG0822">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079283_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="PTHR10093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU_N" evidence="1">
        <location>
            <begin position="6"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16106" checksum="9170798874F03B95" modified="2014-01-22" version="1">MIDQLYQEIILDHYKHPHGKGLRAPFGGEAHHVNPTCGDEVTFRVAVDGSSFSDISYDGIGCSISQASASVLYELLQGVSVDEATQVHEAFVALMQSRGTIEPDEEVLGDGVAFAGVARYPARVKCALLPWMAFKDASARAGVGVSPEVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUF2</accession>
    <name>U5VUF2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative sugar kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358312.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04885"/>
    <dbReference type="EnsemblBacteria" id="AGZ39266">
        <property type="protein sequence ID" value="AGZ39266"/>
        <property type="gene ID" value="AFR_04885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.986"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_2_11"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="391" mass="41462" checksum="28CA4B071B40BB0B" modified="2014-01-22" version="1">MRAGPSQEEVRRHNLGTLLRYVHVHGDTSRAELTTKLGLNRSTIGALTSELAAAGLVTERPPRQTRRAGRPSLVVRPESSQVYAYALAIEVDRLRAARVGLGGKILDRRETERPRGMQVIDAIQPLAGFIHEMRLDVPDGARYVGGGVAVAGMVRRADGMVRLAPTIGWVEEPVGEALSEEIGDVGPLVVGNIADVSALVEHSRGAAAGRDNVIYLYGDVGVGGGIIAGGRRVTGHGGYGGEVGHMVINPHGRPCSCGSRGCWETEIGEFALLELAGRGHLSGREAVLGVVDAAMRGDWAAQKAVRHVGDWIGLGVGNLINIFNPEAVIFGGTLRDVYLVAAAQIRSRLNEVALPACREHVRLRTPELGTDAALIGAAELAFERLLADPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUJ9</accession>
    <name>U5VUJ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphodiesterase/alkaline phosphatase D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_05135</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358836.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05135"/>
    <dbReference type="EnsemblBacteria" id="AGZ39316">
        <property type="protein sequence ID" value="AGZ39316"/>
        <property type="gene ID" value="AFR_05135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1039"/>
    <dbReference type="eggNOG" id="COG3540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015982_2_1_11"/>
    <dbReference type="OrthoDB" id="165142at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032093">
        <property type="entry name" value="PhoD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16655">
        <property type="entry name" value="PhoD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD_N" evidence="3">
        <location>
            <begin position="3"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD" evidence="2">
        <location>
            <begin position="56"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="446" mass="49966" checksum="F1786B50A994CEF1" modified="2014-01-22" version="1">MTTADPRLGHSVHVDVRGLRSDAVYYYRFRAGTQVSPVGRTRTAPGSRSKPRNLRFAFASCQDYQAGFYTAYQHLAAEDLAFVAFLGDYIYEGAPNPNAARQHEGAGEPYSLAEYRNRHARYKSDVNLQAAHAAFPWIVTFDDHEIDNNWADEIPQDPALQTPEAFRARRIAAFQAYYEHMPLRRSSLPAGLDMQVYRRLRFGSLASVHVLDTRQYRSDQPATLTAAQDPALTMTGPEQEKWLVDGLESSGSRWNLLANQVMWASNDRKAGPEQSFDFDNWDGYRVQRKRLLEGFGRVDNPVVLTGDRHCTWVCDLRPDFDDPSTPVVGAEITGTSITSGGNPDVAGFHATYDPIMAESPHWRYIDNQRGYIVCDVNAERMLSSLRLVDTIWATSATVRTAAEFVVEAGRPGIASVSEEQALLRRQPPAGPIYDVHDDQDAHPLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUS5</accession>
    <name>U5VUS5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360599.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11525"/>
    <dbReference type="EnsemblBacteria" id="AGZ40594">
        <property type="protein sequence ID" value="AGZ40594"/>
        <property type="gene ID" value="AFR_11525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2349"/>
    <dbReference type="eggNOG" id="COG3214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043035_1_0_11"/>
    <dbReference type="OrthoDB" id="1594634at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009351">
        <property type="entry name" value="YcaQ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06224">
        <property type="entry name" value="HTH_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="359" mass="40422" checksum="CEC134639FD2116D" modified="2014-01-22" version="1">MALRKLDLDEARRIAVRAQLLDLPRPSDLLAVARQLTLLQIDPTAAVAPNVDLVLWSRLGSAYRPEQVKQALESDRTLFEHNAMVRPMSDLGLYLAAMTVWPYRESHKAWLAENDTFRRDILARLAASGPLVSRDIPDTSAVSWPSSGWTNNRNVTQMLEFMMMLGEVAITGRRGRQRVWDLAERVHPAGTPVVELAEAQRIREHRRLTSMGITRTTNRGTGMEPGAVGESGEEVTVEGTRGVWRVDPEAIGKPFTGRTALLSPFDRLIHDRVRLAELFGFEYVLEMYKPKAKRRWGFFALPILFHDRLIGKVDAKADHKAGVLRVDAIHEDVPFAAAMREGVEAELHDLATWLRLKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUT4</accession>
    <name>U5VUT4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_12335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40753">
        <property type="protein sequence ID" value="AGZ40753"/>
        <property type="gene ID" value="AFR_12335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2504"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143972_0_0_11"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="2">
        <location>
            <begin position="6"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="136" mass="15076" checksum="1C1471E7B2837AE7" modified="2014-01-22" version="1">MTFSVVNRLIDAINAHDLDALAETFADGFASEWPAHPNRSFDGSEHVRKNWGMIFAQFPGIHITVTGSVESDDEFWGEFHYLRPGASDMRGVILIKVLDDKIVQSRFYMEEVEAGTVPHPMPGVSSGQNRSDSAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV48</accession>
    <name>U5VV48_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_06380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359096.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06380"/>
    <dbReference type="EnsemblBacteria" id="AGZ39565">
        <property type="protein sequence ID" value="AGZ39565"/>
        <property type="gene ID" value="AFR_06380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1294"/>
    <dbReference type="eggNOG" id="ENOG502Z957">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051674_3_0_11"/>
    <dbReference type="OrthoDB" id="1828250at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="265" mass="27508" checksum="F3E71CDF5389C53F" modified="2014-01-22" version="1">MKHLIKAEFRRLFATRMWGGLLLGAVLIGGGLMGAMALVGPENFDPPMPGLDTETGIRSVLGMLGYTAFVPAAVGTLAVTSEYRHRTAAVTFLFAPRRWQVLAAKLATYGAAGLAYGLILAGTAATALFAVAFLRGIPLGLPADTVIALLARIGLAMVVYLLLGVGVGALLRNQIAALCIVVGYLYVGEPLLMMIPGVNQLYPVLPGGAMASLTDFTYLADAISQEVGSTAVQLLPPAAGALLLTAYALAAALIAVVLPMRRDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVT2</accession>
    <name>U5VVT2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_13180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13180"/>
    <dbReference type="EnsemblBacteria" id="AGZ40922">
        <property type="protein sequence ID" value="AGZ40922"/>
        <property type="gene ID" value="AFR_13180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2676"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="319" mass="34076" checksum="1E07F51C0A0018CE" modified="2014-01-22" version="1">MAERAQVSISTVSNVLNKPDKVRAATRERVLSVVDELGFIPKVQAVSLARQGTGRIGVMAPFTSYSSYLRRLSGVLTAATELETDVLVFDHESAALTSSPTLASMPIHGRLDGLIVMGLQIEPGIAERLRGRGLPTVMVDADSDLFSRVVIDDDNGGRLAARHLLEQGHRRLGYLLERQVSDYESQAVKRLAGFREAAARQGAEVVVATSGNSVDEARREAAHLLDHADRPTAVMAHHDVLAVGVLLAARDRGLRVPEDVAVMGFDDGEAAAAADLTTIRQPFEESGSTAFGVLLGHIGGSALRSATLLDVQLVERSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVY1</accession>
    <name>U5VVY1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative phosphate starvation-induced protein PhoH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_07955</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359411.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07955"/>
    <dbReference type="EnsemblBacteria" id="AGZ39880">
        <property type="protein sequence ID" value="AGZ39880"/>
        <property type="gene ID" value="AFR_07955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1624"/>
    <dbReference type="eggNOG" id="COG1702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051654_2_1_11"/>
    <dbReference type="OrthoDB" id="445854at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoH" evidence="3">
        <location>
            <begin position="119"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="353" mass="38588" checksum="9F5D04C7FACAE98C" modified="2014-01-22" version="1">MTGTDPSNAAARVQTKISVSDPKIMVNLLGAKDEILRLIERTLTSDVHVRGNEITLTGAPADNATAERLFSELIELIEKGETLSVDAVRRTVAMLEQNTAERPAEVLTLNILSRRGKTIRPKTLGQKRYVDAIDENTIVFGIGPAGTGKTYLAMAKAVQALTAKQVNRIILTRPAVEAGERLGFLPGTLNEKIDPYLRPLYDALHDMLDPESIPRLMQAGTIEVAPLAYMRGRTLNDAFIILDEAQNTTPEQMKMFLTRLGFGAKIVVTGDVTQVDLPGGTTSGLRIVREILQGVDDVHFSELTSSDVVRHRLVAEIVDAYARYDADLEQQATQSVHAVQGRAASGGRPNRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWC2</accession>
    <name>U5VWC2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_08360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359492.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08360"/>
    <dbReference type="EnsemblBacteria" id="AGZ39961">
        <property type="protein sequence ID" value="AGZ39961"/>
        <property type="gene ID" value="AFR_08360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1701"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_545902_0_0_11"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666142" evidence="1">
        <location>
            <begin position="22"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="2">
        <location>
            <begin position="240"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="499" mass="51284" checksum="987AA1060E0AAA03" modified="2014-01-22" version="1">MTLDLLVRTAAVLTLVPTLFAGPPAPEPTALAVSQALVAAFGSDAVVDDSQDTHCQSAPAMPVAYRTDRIILRPPATMTTPTALARLAAALQAEMGAGTFTIGTPEVLEWNEPAAARLDDSPLRKVISVPVGSVSAEDVPVLRVARRLVRLDHLPAAPDYLVGPSNGPLGVWPDGGPEKTGSPGVPRPGLGAGTTVALFDSGVPASIEAKLPPRLSALTAGDAEDPDRNDDDLADVYYAVHLTAIAGIFATIVPEADVVGVRVTGPNGVATEFSAAKRMVTTLRAAHRAGAWPQVIVNSFGTAVCHEGGPNPGVDLIPLGLQLVAEAVDRHQEAVVVAAAGNRGTDKPFYPAAFQGGVESVISVGALDATADNDGNAWTSKSRTAKPASFSNYGDWVTAWAPGVDLATYHAVGLRFEPGGELIDGYAKVSGTSFAAPFVGAEIAEELARHSGSPLEAWRAVRDAGRPCSAEVGSGVAVALSSMDSRSTTPADRGLPAEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWI3</accession>
    <name>U5VWI3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_14300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361201.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14300"/>
    <dbReference type="EnsemblBacteria" id="AGZ41142">
        <property type="protein sequence ID" value="AGZ41142"/>
        <property type="gene ID" value="AFR_14300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2902"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="299" mass="31844" checksum="6BBC824A3912A8FA" modified="2014-01-22" version="1">MNDSALHTVNLTKRYGSVTAVDELSLDVPAGEVFGFLGPNGAGKSTTIRMLLGLARPSAGRAWVFDTDAADVARAHRLLAYVPADVALWPSLTGAEILHLQARTGSGTDLAYRDELVERFALDTTKPARTYSSGNRQKVALVAAFATRAPLLVLDEPTSGLDPLMEREFRTAVAQARERGQTVFLSSHQLSEVESICDRVAILRGGRLVEIAALDQLRGLHRAEVTVSYTGAPPPGLDTIPGVDAVEQVGAGRLRFSLIGSPAPALQALAAADVTALAIREPTLEEIFLDYYGAAEGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWT3</accession>
    <name>U5VWT3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361311.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14850"/>
    <dbReference type="EnsemblBacteria" id="AGZ41252">
        <property type="protein sequence ID" value="AGZ41252"/>
        <property type="gene ID" value="AFR_14850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3015"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_1_1_11"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="30997" checksum="EBACF99FF52E6FE0" modified="2014-01-22" version="1">MDRAQLADFLRARREALQPEDVGLPRGARRRTGGLRREEVAALSGMSADYLGRIEQQRGPLPSEQMLAGLARGLHLSLNERDRLFALAGHPAPRRVLRDDHLSPSMMRIVDRLTDTPALVMSRFGETLLQTRPAVALLGDFMRFTGLSRYLVYRWFTDPATRELYPPEDHNLRGRVFTVDLRTAYTAAPEGRAGEIVAALLEVSEEFAEIWRLHEVDVTHHHDLKRYRHAELGELELYCQPLVDPEQAQELLVFTAIPGSPSHEKLQLLAAVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXF8</accession>
    <name>U5VXF8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_16285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361614.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16285"/>
    <dbReference type="EnsemblBacteria" id="AGZ41539">
        <property type="protein sequence ID" value="AGZ41539"/>
        <property type="gene ID" value="AFR_16285"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16285"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3304"/>
    <dbReference type="eggNOG" id="ENOG5032U5G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1576693_0_0_11"/>
    <dbReference type="OrthoDB" id="1719693at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="165" mass="17790" checksum="33C1D374FBB57174" modified="2014-01-22" version="1">MTRDGVAYERRSAAPVIAPVWPRKLAKVPFVELAEGRLQGVVSSGSDPSRVYVSSITAREHGLSCNTNNNRPCGGLSGAHPCNHIGALIDAAVAQYGLDRVTRYLKVELSEGDENVRAALHPTAAPTRAPEVFSSFLRHLAYLELPPTTDPLPELHWFPAAGAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXZ6</accession>
    <name>U5VXZ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase S8 and S53 subtilisin kexin sedolisin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_17405</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361992.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17405"/>
    <dbReference type="EnsemblBacteria" id="AGZ41759">
        <property type="protein sequence ID" value="AGZ41759"/>
        <property type="gene ID" value="AFR_17405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3528"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_579928_0_0_11"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase_S8" evidence="4">
        <location>
            <begin position="182"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="448" mass="46638" checksum="4CC54B3CB8D63FD7" modified="2014-01-22" version="1">MTQNWPLPDHRTAGQIAQDQVAVRAQVANLVSPAADVAARRDLDGIVERIRRRQLAATDEHATRFVSFSPSDGAGPLLCAEGELVIDGGAAQAPRLRGLLSGFAAADRTDRSARRRTRVVRSTSARSFEELRAEAKRLRDDHNVMANVNAIVPLGYIVKGNSYPGATTAPPPFTSNGGGAPVRVAVIDTGLTATPRTDSWDAGMVREGTDLVNVLAPLDRIDWFAGHGTFAAGIVRQIAPDCEVVVYRFTCTDGLGTDEAAADMMIRAADEAAGQRLIINASFGAPAVDGIPPLAMREAVEYITETYPKVLIVASAGNDGLDEPLYPAAFPGVKAVGALNSDLTPAAFSNHGSWVQCSTVGVGIVSTFVEGKLPPEDNIGTDVTFPANSWATWSGTSFTAPQIAGAAAALCGEDGALLPSAAFDQLVAAGDPVPGLGAAVHLLPGTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYQ1</accession>
    <name>U5VYQ1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4097 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_12525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360850.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12525"/>
    <dbReference type="EnsemblBacteria" id="AGZ40791">
        <property type="protein sequence ID" value="AGZ40791"/>
        <property type="gene ID" value="AFR_12525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2544"/>
    <dbReference type="eggNOG" id="COG3595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081211_1_0_11"/>
    <dbReference type="OrthoDB" id="1059710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025164">
        <property type="entry name" value="DUF4097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13349">
        <property type="entry name" value="DUF4097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4097" evidence="1">
        <location>
            <begin position="16"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="221" mass="22762" checksum="D7325A72673FC2E8" modified="2014-01-22" version="1">MQTFATPAPIAAVLDIPAGRIQFIAAERADTVVEVRPADPGRSRDVRAAEQTTVTYADGALRIESSQPSNRLVGPTGSVEVTVQLPAGSRVEGRSASTELRGVGRLGDVSFEGAYRRIKIDEAANVRLTAIDGDVEVGRLTGPAEISTGRGDIRIAEAVQGTLLLTTQQGDITVAAATGVSATLDAGTALGRVSNALKNDGTAGLDIRATTAQGDITARSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZD4</accession>
    <name>U5VZD4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_19860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19860"/>
    <dbReference type="EnsemblBacteria" id="AGZ42244">
        <property type="protein sequence ID" value="AGZ42244"/>
        <property type="gene ID" value="AFR_19860"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19860"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4030"/>
    <dbReference type="eggNOG" id="ENOG5030RC6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_768656_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665379" evidence="3">
        <location>
            <begin position="25"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="359" mass="36592" checksum="DF51498BAC748D30" modified="2014-01-22" version="1">MWRRSGLAVLAVGAALLTATPAVAAPPATVDLRATIDGAAEAVPGELLYVRIGTVLAGSQTPTGVKMTVTLPEGVSYQGDGGAASDGAGPCEGEIGGRTVVCIPEPTEVTQGGVWWQAPGRVAESVPIGTELTTTVVVSSDSEETNPADNTATLTTRVAGPAATFLKVTGPAEPVVPGQPVRIKVRLHYGEATPVDEFVMLGSFGAWFIGGGGITNIPADCWADGSLTCQVPRRLESGTDLDLIFELKSSVRPDDYRELRDTMSFSLEYLEYPIRATGTVTFTKAPSPSPTPSSPSPSPSPTGTGTGNGGESGGDDEQASGGLPITGAPIAALATAGLLLITAGAGALLLSRRRSRATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZM8</accession>
    <name>U5VZM8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative copper resistance protein CopC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_14050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14050"/>
    <dbReference type="EnsemblBacteria" id="AGZ41096">
        <property type="protein sequence ID" value="AGZ41096"/>
        <property type="gene ID" value="AFR_14050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2850"/>
    <dbReference type="eggNOG" id="COG2372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087859_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032694">
        <property type="entry name" value="CopC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007348">
        <property type="entry name" value="CopC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34820">
        <property type="entry name" value="PTHR34820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04234">
        <property type="entry name" value="CopC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665920" evidence="4">
        <location>
            <begin position="26"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="CopC" evidence="5">
        <location>
            <begin position="24"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="198" mass="20449" checksum="3A4A356722D089C5" modified="2014-01-22" version="1">MRKALAALAAVVIVLLPAAPAWAHAQLVSADPAEDASLARAPASVTLTFSERLNPEFTTIVVSDAARQRIPAAAPTVDAGKGTVSLTQPLSNGAYTVAYRVVSVDGHTVQGSYPFTVADPTRPAAAAASQPAVARADRSTGISTPVLIGLGAFGVLCVLVVAYLYVSGRRRAARREQVVPARDGDVVQPAQTGRLARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZP9</accession>
    <name>U5VZP9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chitinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_20980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20980"/>
    <dbReference type="EnsemblBacteria" id="AGZ42468">
        <property type="protein sequence ID" value="AGZ42468"/>
        <property type="gene ID" value="AFR_20980"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20980"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4253"/>
    <dbReference type="eggNOG" id="COG3179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045506_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665529" evidence="3">
        <location>
            <begin position="24"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_19_cat" evidence="4">
        <location>
            <begin position="42"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="101"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="229" mass="24310" checksum="BDCBF198BD3DBB00" modified="2014-01-22" version="1">MLAAAVAAAAALTATLVTTSANAATFPVSPAQFDQMFPSRIAFYSYDGLLDAMQKFPAFTGSGNDTAQKQEAAAFLANINHESGGLVYVEEINQDNWPHYCDAAQPYGCPAGQSAYHGRGPIQLSWNFNYKAAGDALGIDLLNNPDQVKNDSSTAWQTGLWYWMTQTGPGSMTPHDAIVNGAGFGETIRSINGSLECNGGNPAQVQSRVDAYQRFTQILGVEPGGNLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W026</accession>
    <name>U5W026_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_20550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362754.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20550"/>
    <dbReference type="EnsemblBacteria" id="AGZ42382">
        <property type="protein sequence ID" value="AGZ42382"/>
        <property type="gene ID" value="AFR_20550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4167"/>
    <dbReference type="HOGENOM" id="CLU_1773415_0_0_11"/>
    <dbReference type="OrthoDB" id="1878314at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16226" checksum="75946FC7E37EA2CF" modified="2014-01-22" version="1">MLVQLDRNETNRTFTPNSTAVPLPDLNTQRHSPVVAVLGYPGRVAGLAAHLPAGWTLRHPAGLDDIRAGEIVLISGATVHDVSTARALLPARSRIVTLVDDDAPAELVAGVLTAGADVCVRGGLPAILASHLVACRRRQLADRWNDVQDALPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0A7</accession>
    <name>U5W0A7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362839.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20975"/>
    <dbReference type="EnsemblBacteria" id="AGZ42467">
        <property type="protein sequence ID" value="AGZ42467"/>
        <property type="gene ID" value="AFR_20975"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20975"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4252"/>
    <dbReference type="eggNOG" id="ENOG5034BG1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095281_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="227" mass="23946" checksum="5263BFC4300E469E" modified="2014-01-22" version="1">MTSALQRLLASRGIGDAGSSPAVLRRLALVLGLHTPDLFVIAGQPLPADLAPARGPRAHSVGLLVTGALALRPGDRREVHEFVRSLPVHRPAPEATPADPADGPGPLLVRLLRNRNIHPANAELLLLMGGPYVSDSTVVLLGQGEVEVTPQLVSGFAGILGLHPDDLAALTGVRPDPESRPHPYAPELSRLAWDARRLTGEQLSEVFRLVDSLSRAAGPPIVRTVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0K7</accession>
    <name>U5W0K7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Winged helix family two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_21845</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363010.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21845"/>
    <dbReference type="EnsemblBacteria" id="AGZ42639">
        <property type="protein sequence ID" value="AGZ42639"/>
        <property type="gene ID" value="AFR_21845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4427"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_8_11"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="125"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="125"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="24929" checksum="30249DB4F52CC07E" modified="2014-01-22" version="1">MSRILVVDDDDQLRRALRINLAARRYEVLTAADGTSALRTAGHTPPDLAIVDLGLPDLDGVEVVRGLRGWTSIPIIVLSARHDQAGKVEALDAGADDYVTKPFGMDELLARIRAALRRAAPQPDSVTITTAAFTIDLAAKRVTTTAGDEVRLTPTEWHMLELLARHPDKLVAQKQVLREVWGPAYENETGYLRVHLANLRRKLEPDPSRPRYLITEPGIGYRFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0M6</accession>
    <name>U5W0M6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="3">SDH</shortName>
            <ecNumber evidence="3">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aroE</name>
        <name evidence="6" type="ORF">AFR_16070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361571.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16070"/>
    <dbReference type="EnsemblBacteria" id="AGZ41496">
        <property type="protein sequence ID" value="AGZ41496"/>
        <property type="gene ID" value="AFR_16070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3261"/>
    <dbReference type="eggNOG" id="COG0169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_4_3_11"/>
    <dbReference type="OrthoDB" id="1054867at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate_dh_N" evidence="4">
        <location>
            <begin position="7"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="SDH_C" evidence="5">
        <location>
            <begin position="250"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate binding" evidence="3">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="130"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="29635" checksum="E83BA39458CB49AE" modified="2014-01-22" version="1">MGSRCGLIGAGIGTSLSPALHEAEARSQGLDLTYELFDVTRPDTPAELGRTLDEAERDGFAGLNITHPFKQQVIELLDHLSPQAAAIGAVNTVAFRDGRRYGYNTDAYGFTESFRANLPGAATGRVVQLGAGGAGAACAYAQLSLGVTHLTIADPDAGRRDQLAAALATRFDRARITAVAPPELPVVLADADGLVNASPAGMHDHPLLPLPAELLHRGLWVFDIVYMPVETALLRAARAAGLRALGGAAMCAYQAAAAFELFTGRTPDTTRMLRHLDVLLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0S0</accession>
    <name>U5W0S0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydrodipicolinate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21635"/>
    <dbReference type="EnsemblBacteria" id="AGZ42597">
        <property type="protein sequence ID" value="AGZ42597"/>
        <property type="gene ID" value="AFR_21635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21635"/>
    <dbReference type="eggNOG" id="COG0329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_7_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="355" mass="35680" checksum="B36B4AA153EB3CFF" modified="2014-01-22" version="1">MLGRDHAGIMTFTGLYVPLITPFETTGGVALDALEQLARDVLAAGARGLVALGTTGEPSALSDAEQQAVVDVAARVCREHSASLIVGSTSATVPPDVTARLVLVPPFVRPGEDGVVAYFAAQAGDVPVIVYHVPYRTGQELSAGSLRRIAAVPCVAGIKYAGGGISAATADLMADPPDGFAVLGGDDAVISPLLAMGAHGGILASAHLATAGFAELIAAWHAGDTVRARALGNRLAPLSLALFAEPNPTVIKGVLHATGRIPSAAVRLPLLPAAPESVQAALRLLEGLADRGDQAGGPGVAVTDHPARHVGEADAVLVVAERELAARAVVAEAALPQQGTEAGGVEQEAGSPAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0X4</accession>
    <name>U5W0X4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GAF sensor hybrid histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_23215</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363438.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23215"/>
    <dbReference type="EnsemblBacteria" id="AGZ42913">
        <property type="protein sequence ID" value="AGZ42913"/>
        <property type="gene ID" value="AFR_23215"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23215"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4705"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_796038_0_0_11"/>
    <dbReference type="OrthoDB" id="683795at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="4">
        <location>
            <begin position="268"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="348" mass="36687" checksum="F5905290AFAF9420" modified="2014-01-22" version="1">MPPHTEFRLDEAVADVYGDLRLAPVLRRLLRHTGRLTGSVAGSVSLIDADHGRYVKAAEYGASCRLGHSFPLDEGATGRAFGSRRPVVIPDYGQLRAGHLAAAHPARQGPAAAVPIWWRGEVIAVSVAFGPDAGALTTRGVDELEALTQSAAAAIVECGRRHPSLAAVIRRRFGDTPARAVVTEAGLVRPVSPEVARVAADLVAEAGRAAPTLHVAIVYRQEGVRVLVHAAPAPGAYDQLLRTAGGGVTVEQVHGWGVVVRADLPYAAPAAPAPFTPREAEVLELLRDGLTYREMARRLGLSAKTVEKHVAAILRKTGASNRTTAVVTALDNGWMGNLPHTAAYTKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W155</accession>
    <name>U5W155_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363578.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23600"/>
    <dbReference type="EnsemblBacteria" id="AGZ42988">
        <property type="protein sequence ID" value="AGZ42988"/>
        <property type="gene ID" value="AFR_23600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4782"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_14_0_11"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="270"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="36676" checksum="8AEBAD8C28B880F5" modified="2014-01-22" version="1">MQQRLDRVELADGTTVAYASAGAGRPLVYVMGWLTHLERGWELPAERALYEALARGCRLVRYDRAGCGLSAVVDRPASLEFELEQLAAIAAVLDEPFDLMGTSMGAPVAVAWAAARPATVRRLVLYGGWAHGADLSPPGVREHVLGLIASHWGLGSEVLTDIFAPDADRSTRAELARYQRESSSAATARELLTLSYELDVRDRLDHVRAPTLVVHRTGDRAAPLSQAEVLAAGIPRAELVRLPGRSHLPYAGDRDELVRVVRRFLGLGAPRHRTGGLTARQREVAELVSQGCTNREIAARLGITERSAEGHVERIRLRLGLRSRTQLAAWWTAQVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W184</accession>
    <name>U5W184_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361915.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17210"/>
    <dbReference type="EnsemblBacteria" id="AGZ41720">
        <property type="protein sequence ID" value="AGZ41720"/>
        <property type="gene ID" value="AFR_17210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3490"/>
    <dbReference type="eggNOG" id="COG3655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_31_8_11"/>
    <dbReference type="OrthoDB" id="2040098at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13443">
        <property type="entry name" value="HTH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="75" mass="8029" checksum="7EC268660014A4D1" modified="2014-01-22" version="1">MPIVVRIDVELAKRKMSVGEFAEAIGLTPANVAVLKNGRAKAVRFTTLEAMCRVLDCQPGDLLEWVDDEDAGAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1D3</accession>
    <name>U5W1D3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ42954">
        <property type="protein sequence ID" value="AGZ42954"/>
        <property type="gene ID" value="AFR_23420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23420"/>
    <dbReference type="HOGENOM" id="CLU_1657077_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="159" mass="16195" checksum="91FCA8FBA33E73C0" modified="2014-01-22" version="1">MILTGTVPFGSGSCRTSWAVPPVIPTPATRPSRRSTNCSEEPAGSLSTHATLALSWISAPTVPGSSVRISSSREPLSDSVTLGTAPRSIVAMSKRTSPRSTLRMRRTVDAPTTSSCRSRTAPSGAVTRCAASGHGSAAWLGDGSTSAGTGAAWAPAAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2A0</accession>
    <name>U5W2A0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_24355</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24355"/>
    <dbReference type="EnsemblBacteria" id="AGZ43137">
        <property type="protein sequence ID" value="AGZ43137"/>
        <property type="gene ID" value="AFR_24355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4934"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_11_0_11"/>
    <dbReference type="OrthoDB" id="460405at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="217"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ43137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="420" mass="42809" checksum="DADD59EE442E6544" modified="2014-01-22" version="1">MSALADLRPLRESPPFRRLWLGTTASGFGGQLGAFAVVYYVWEHTRSAAMVGLVGLVTAVPMIVLALAGSAFMDHVDRRLLAFRCSCGQLVVSLGMAAVAATDIGGVPLMLVLAGLGSAFASVGGPAKRTFIPALLTGDRLAAGLALSHLSFQLAMLLGPATAGLVTAQWGTTWCFVIDAVTFAFALIGIAGLPRGVESAEGRPGAAAVVDGFRFTARTPEVRGAFLADLAATMLAMPFALFPLINAEKFGGRPEILGLFTTAVAVGGVLASVLSGLATRRRHPGTVLLVCGTVWAVSLGFVGLTGHLLIVLSLLAVAGAADTWAVVSRGTVVQTATPVAYRGRVASLEHIVGSAGPELGNLRAGLVATVTSGAASLALGGILSVIATGLIAVTTPALRRFTVPEVEPEMHDRSAGVPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2B1</accession>
    <name>U5W2B1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25570</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364022.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25570"/>
    <dbReference type="EnsemblBacteria" id="AGZ43378">
        <property type="protein sequence ID" value="AGZ43378"/>
        <property type="gene ID" value="AFR_25570"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25570"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5182"/>
    <dbReference type="eggNOG" id="COG3266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037716_1_0_11"/>
    <dbReference type="OrthoDB" id="1644627at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665616" evidence="2">
        <location>
            <begin position="22"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="307" mass="33905" checksum="3AD99D02B1913986" modified="2014-01-22" version="1">MRAFVLAILVIAALTGCSNQSDDKKVVASPAPSAVPSQPPTEAAPAKPQELPLGGTKIFPAYRVVAYYGTPGSGTLGVLGEGSPDKMLPKLRKAAKPFAGDRKVQVAYELIASVAQAAPGKDGDYSQMISLDKIEQYVDQARRNKVLVILDLQPGRGDFLPQARQLERFLVQPHVGLALDPEWRMPKGKVPGKTIGKVDAAEVNRVSGYVSGLVQRHRLPEKLFVLHQFRSSMLPDVAKIQKRPGLALVQHVDGFGTRSEKDATWDRLRRPQQFHLGYKLFYDEDVKRYGPRDLLEFKNVPELISYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2K1</accession>
    <name>U5W2K1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidoglycan-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_25475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363984.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25475"/>
    <dbReference type="EnsemblBacteria" id="AGZ43359">
        <property type="protein sequence ID" value="AGZ43359"/>
        <property type="gene ID" value="AFR_25475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5161"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590045_0_0_11"/>
    <dbReference type="OrthoDB" id="1584918at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13517">
        <property type="entry name" value="FG-GAP_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665622" evidence="2">
        <location>
            <begin position="33"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="463" mass="49626" checksum="7533B53E7E84E835" modified="2014-01-22" version="1">MLPIVLRRVLITLVTTTVTAAGLAVGVSPAAAAPPTPSFGADIENWAPDDQADTCDPVNKPGTTAFMNLLNDTYGRHASAENISRACGGSRSEHHEGRALDYILNYNDSGQRADAEEIIAWLLATDRYGNQAANARRLGVMYIIWNRRIWSQARHTEGWRNYPCNGEPGDCHTNHVHFSFTWAGARQQTSWWSGVTHRPTGTVVDVSGDGFGDLLTVRADKTLWYYPNNIGRDGVPFQGDGVEVGRGFAKPGLISSADISGDGYADLLAVTADNKLAYYPNNIKRDGKPYGTPTVFGQGFADTTRILLADVSGDGFADLLTTTSNGRLHLFPNNIGRDNGIPFANSTPVGQGFAATAKLTAGDITGDGYAELLAATADGKLAYYPNNIKRDGTPYNTAVPIGEGWNAVSNLRLSDVTGDGYSDVLGVRTDGALSLYPNNIERDGIPFANSTPVGNRFTVVHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W395</accession>
    <name>U5W395_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_20990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362842.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20990"/>
    <dbReference type="EnsemblBacteria" id="AGZ42470">
        <property type="protein sequence ID" value="AGZ42470"/>
        <property type="gene ID" value="AFR_20990"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20990"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4255"/>
    <dbReference type="eggNOG" id="ENOG502Z7P1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025722_0_0_11"/>
    <dbReference type="OrthoDB" id="826678at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025566">
        <property type="entry name" value="DUF4331"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14224">
        <property type="entry name" value="DUF4331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="468" mass="51435" checksum="225938FE2C845D28" modified="2014-01-22" version="1">MSSHREAPEISKDPVADSADLYAFVSPDHPDTVTLIANYVPLQLPASGPNFFEFGDDVLYEIHVDKNGDGRPDLTYQFRFRTELRNDRTFLYNTGPIESLDSENWNRRQFYSVTRVDASGKHTVLAEKLPCPPCNVGPLSIPDYDKLAEDAVHKLKTGEKVFAGQRADPFFVDLGAIFDLGTLRPFQDKHLVGAKLFNYAGKAVNATDKMNVHSIAIQLPLHDVRRDGKKKVRGRDPEAVIGVWTSASRRQVQVRDGRKNGDDVYVGPQVQVSRLGNPLFNEVIVPMAQKDLWNSLPPSEDKRFAEFVEQPELGALLPVLYPGLFDNLAELNKAKTARADLVAILLTGIPDGLIDDFQNNTGDVQADMLRLNTAVPPTKEPNAFGILGGDLAGFPNGRRVADDVVSISLRAIAGVTVPLVDEKFTADDAAALVEQGLSAKDASSKLLKKFPYLGVPFDGFNNPEAEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3D7</accession>
    <name>U5W3D7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_21190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362882.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21190"/>
    <dbReference type="EnsemblBacteria" id="AGZ42510">
        <property type="protein sequence ID" value="AGZ42510"/>
        <property type="gene ID" value="AFR_21190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4296"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_11"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="PTHR43150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="2">
        <location>
            <begin position="28"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="36778" checksum="223160457264E019" modified="2014-01-22" version="1">MTYSAAEDRYDTMTYRRAGRSGLKLPAISLGLWHNFGDGRPWERQRDITRRAFDLGVTHFDLANNYGPPPGSAESNFGRILGTDFKHHRDELVVSTKAGYHMWNGPYGEWGSRKYLVSSLDQSLGRLGLDYVDIFYHHRPDPDTPLEETMSALDAIVRSGKALYVGISNYNSEQTAQAAAILQELGTPLLIHQPSYSILNRWIEKDHLLDTLEAVGAGCIAFSPLQQGLLTDRYLGGIPDDSRVRTSVFLNESDLDKGTMGRIHNLNDIAKRRGQSLAQLALAWALRDQRMTSLIIGASSVEQLETNIAALGNLELSSDELNEIDGTSLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3E6</accession>
    <name>U5W3E6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26865"/>
    <dbReference type="EnsemblBacteria" id="AGZ43634">
        <property type="protein sequence ID" value="AGZ43634"/>
        <property type="gene ID" value="AFR_26865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5444"/>
    <dbReference type="eggNOG" id="ENOG5033YRJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1222685_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="21100" checksum="C5F47581A6A29B4D" modified="2014-01-22" version="1">MRGRTGYAGLLCVLGAAIGAAGGLALVFVTPAVTPDRFSYPLSPGAHRLAEASFAVSHVLLLIGVLGLVWAGATGGTRLGRAGITTTVAGLAVLTGCEVGAYALADSAYPTTATDRLEAGYGVSTILIGVGLVMAGVAVVRARGLAGHARYLVLICGVAVFVVVLPGVMGPMVAGRLALVAWMVLWGVLGVALIRHDRSPSFQHSVAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3F3</accession>
    <name>U5W3F3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hydrogenobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="3">6.3.5.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Hydrogenobyrinic acid a,c-diamide synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cobB</name>
        <name evidence="6" type="ORF">AFR_26915</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of hydrogenobyrinate, using either L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 ATP + 2 H2O + hydrogenobyrinate + 2 L-glutamine = 2 ADP + 2 H(+) + hydrogenobyrinate a,c-diamide + 2 L-glutamate + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:12544"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:77873"/>
            <dbReference type="ChEBI" id="CHEBI:77874"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 9/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP and hydrogenobyrinate and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">The a and c carboxylates of hydrogenobyrinate are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CobB catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CobB/CbiA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.9" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364546.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26915"/>
    <dbReference type="EnsemblBacteria" id="AGZ43644">
        <property type="protein sequence ID" value="AGZ43644"/>
        <property type="gene ID" value="AFR_26915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5454"/>
    <dbReference type="eggNOG" id="COG1797">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022752_1_1_11"/>
    <dbReference type="OrthoDB" id="692368at2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00220"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043802">
        <property type="term" value="F:hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="PTHR43873"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="4">
        <location>
            <begin position="7"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="GATase_3" evidence="5">
        <location>
            <begin position="252"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="3">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="452" mass="46116" checksum="0BFFFE67D0C3B10E" modified="2014-01-22" version="1">MVSIPRVVIAAPASGHGKTTVATGLLAAFAARGLKVAPFKVGPDYIDPGYHSLAAGRPGRNLDPVMVGEELIGPLFAHGSAGTDLAVVEGVMGLYDGRTGAGDTGSTAHVAGLLDAPVILVVDAAAQGRSVAALVHGFRSFGSVRLAGVILNRVGSDRHEAILREACEEVGTPVLGAMRRADAVAAPSRHLGLVPAAERRSEALASVSALASLVTASVDLDAVLAVARSAPSLPVTPWTPIAPDPVPGRPIVAIAGGPAFTFAYAETAELLAGAGAEVVTVDPLRDEKLPDGTRALVVGGGFPEVYAAELSANEPLRLAVASLAAVGLPIVAECAGLLWLCRTLDDAPMCGVLDADAAMTPTLTLGYRDAVALTDSPLYRAGSRITGHEFHRTTVHPRSGLLLEPAGGAAWAWRGADPEGFATPTLQASYLHLHWAADPGIARRLVAQAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3G5</accession>
    <name>U5W3G5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Proteasome subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">20S proteasome alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Proteasome core protein PrcA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">prcA</name>
        <name evidence="5" type="ORF">AFR_26400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein degradation; proteasomal Pup-dependent pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364345.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26400"/>
    <dbReference type="EnsemblBacteria" id="AGZ43542">
        <property type="protein sequence ID" value="AGZ43542"/>
        <property type="gene ID" value="AFR_26400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5351"/>
    <dbReference type="eggNOG" id="COG0638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071031_0_0_11"/>
    <dbReference type="OrthoDB" id="1243753at2"/>
    <dbReference type="UniPathway" id="UPA00997"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019941">
        <property type="term" value="P:modification-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_B">
        <property type="entry name" value="Proteasome_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022296">
        <property type="entry name" value="Proteasome_asu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599:SF69">
        <property type="entry name" value="PTHR11599:SF69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03691">
        <property type="entry name" value="20S_bact_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="230"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="284" mass="30166" checksum="16AF9E24AFBEFE77" modified="2014-01-22" version="1">MAMQFYASPEQVQRDRSEYARKGIARGRSAVVLTYEGGVLLVAENLTSLRKISELYDRIGFAAVGRYNEFESLRRAGVRMADLNGLAYDRRDVTGRALANAYTQTLGAIFSETQKPYEVEICVAQVGTSPESDELYRITYDGSVQDEPGFMAMGGSAEAISTVLKSRHDVAADLATALALAVEGLASVGGENGTPRTLGASQLEVAVLDRNRKGRTFRRIAGAALTTLLEGGKDEPEKEVGEAGDVAPPAPGEDKPTVSAASVDLEGKNEDPAEPADDTNGDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3H5</accession>
    <name>U5W3H5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BFN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362863.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21095"/>
    <dbReference type="EnsemblBacteria" id="AGZ42491">
        <property type="protein sequence ID" value="AGZ42491"/>
        <property type="gene ID" value="AFR_21095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4276"/>
    <dbReference type="eggNOG" id="COG1259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096111_2_1_11"/>
    <dbReference type="OrthoDB" id="1594801at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036104">
        <property type="entry name" value="BFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003729">
        <property type="entry name" value="Bi_nuclease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02577">
        <property type="entry name" value="DUF151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103256">
        <property type="entry name" value="SSF103256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51658">
        <property type="entry name" value="BFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="154" mass="17056" checksum="2B971D89F98C9A51" modified="2014-01-22" version="1">MRELSVVGVRVELPSNQPIVLLREVDGDRYLPIWIGAVEATAIAYEQQGVKPARPLTHDLLRDILAALEAPLKAVEITELKENVFYADLLIGDNLRVSARPSDSIALALRVGAPIRCADQVLTEAGIVIPDEQEDEVEKFREFLDQVRPEDFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3I6</accession>
    <name>U5W3I6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_26525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364395.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26525"/>
    <dbReference type="EnsemblBacteria" id="AGZ43567">
        <property type="protein sequence ID" value="AGZ43567"/>
        <property type="gene ID" value="AFR_26525"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26525"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5377"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045252_0_0_11"/>
    <dbReference type="OrthoDB" id="1363846at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="332" mass="36945" checksum="5D43C5D6109C8810" modified="2014-01-22" version="1">MAQRFTLDEIRSRTYKDRDAWWTVWLVDPLASRLVWLVAPVRWVTPNVLTMGAFVLGIVTAYCFAQGDYPWLVAGAIIFHLSFVLDCMDGKIARLKGTGSVFGAWLDYVFDRLRVLTCGLALMGGQWAKTHDQTYLWLAAAVIFLDMFRYLNALQMGKVKNDMRRRLEAARGENSGLPVFVEETGIEHPVGTGATATTVTGTTGEERPVIDLYGDFRTKYSAFVKVRNMLVRNRIRAHVFSGIEFQMFIFILGPLTNQIKFFTIFSAVALAAFELLLIYKLWTATRSYVRELAKIGTSEAEAEAVAVATGAEAPGGEDADDPEAITGARITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3U2</accession>
    <name>U5W3U2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_27715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560141.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27715"/>
    <dbReference type="EnsemblBacteria" id="AGZ43804">
        <property type="protein sequence ID" value="AGZ43804"/>
        <property type="gene ID" value="AFR_27715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5617"/>
    <dbReference type="eggNOG" id="COG1725">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_10_1_11"/>
    <dbReference type="OrthoDB" id="1860081at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="117" mass="12480" checksum="43F729C1D0B1B7E4" modified="2014-01-22" version="1">MIVIDASSPTPPYEQLRAQLARQIQDRSLVVGTRLPTIRRLAADLGLAVNTVGRAYRELEDAGLIETRGRAGSFVSAAGEQALEQAHRAARDYASVTSSAGIDPAHALRIVEAALKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Y6</accession>
    <name>U5W3Y6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28335"/>
    <dbReference type="EnsemblBacteria" id="AGZ43928">
        <property type="protein sequence ID" value="AGZ43928"/>
        <property type="gene ID" value="AFR_28335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5744"/>
    <dbReference type="HOGENOM" id="CLU_1212706_0_0_11"/>
    <dbReference type="OrthoDB" id="1728344at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="212" mass="22266" checksum="D56CA56D94C8C176" modified="2014-01-22" version="1">MDGVRSAQEAVTLETLGADLIGVALSPNPRSPGTPVLTEEQAATIADALIHAVLVTSMELTDRNQVLRSVAATGAGLVQTITASIPPPDVRAALREAGVGIVYSGLEINHDDDPGWVFGAFTDVPDLNAALFQVEVLPEYTGSWAFLRDESPEFAAEFQIADLDALGRERPLVVGLDFTTGNVREIIAALPWVRGIALTRGDAVRVLGAVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W483</accession>
    <name>U5W483_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28415"/>
    <dbReference type="EnsemblBacteria" id="AGZ43944">
        <property type="protein sequence ID" value="AGZ43944"/>
        <property type="gene ID" value="AFR_28415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5761"/>
    <dbReference type="eggNOG" id="COG2220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051050_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="2">
        <location>
            <begin position="9"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="281" mass="31012" checksum="B5AF8F5BD881DFB8" modified="2014-01-22" version="1">MKAGVTFVGNATTLLRLGSFTLLTDPNFVPAGTRVYLGKGAWTRRLLDPAMTMGELPPLDGVVLSHLHGDHFDGVAKAELPSATPIFTTPQARRKLRQWKFRAVRGLPTWESYDWARGRERLRITAVPGRHGPGPAEWLLPDVMGSVIELRVDDVCRLRVYITGDTLNRPFLAQVPERCGDIDAMIVHLGGTRVFGMLLTMDARQGVDLTELIRPRLTVPVHHDDYTVFTSPLSDFVERAREHGLTGIQPIERGSTLELPVRPAGQVSRPGLASGAQDSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4C6</accession>
    <name>U5W4C6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transporters inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_22530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363187.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22530"/>
    <dbReference type="EnsemblBacteria" id="AGZ42776">
        <property type="protein sequence ID" value="AGZ42776"/>
        <property type="gene ID" value="AFR_22530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4567"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_11"/>
    <dbReference type="OrthoDB" id="1074175at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="68"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="272" mass="29928" checksum="C7948759A6599670" modified="2014-01-22" version="1">MRRLPFSPWHLLLMPLALIFVLPLVQMVLTSFMTSQEINQFPPKFLPTSIHLDGYRTLLSESNILQWFGNTVLVSAIAVLSHLVLCSLAGYGFARLKFAGRGLAFLAIVATVMIPIQLLMIPTYLMFARAGIVDTLAAAFVPWLASAFGIFLMRQFFLGLPAELEEAAVLDGCSTLRTFLSVVLPLARPALATLAIFTLLSSWNDLLWPLVAISDDSKFTLQVGLASFQGTRRTEWSQLMAGNVIATMPLIIAFLVAQKRFIATMTFSGLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4I8</accession>
    <name>U5W4I8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lactoylglutathione lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29260"/>
    <dbReference type="EnsemblBacteria" id="AGZ44113">
        <property type="protein sequence ID" value="AGZ44113"/>
        <property type="gene ID" value="AFR_29260"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29260"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5929"/>
    <dbReference type="HOGENOM" id="CLU_1458347_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="24"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="185" mass="20390" checksum="620832E808DE8D38" modified="2014-01-22" version="1">MTETVQKLTSFDLLRPAIHPLAQGFFQWCVVTPDLDAKCAELEKLYGPAGFWIMENAPLLNVTYRGKQIDLRVDLALGYIGDTNIEVVRPRPDGDDNLYSEFLEERPEGGFHHLGFQVYDFEAAAESLTESHGAAVQSGDFGVDGTVFAYFDTTAVTGLYTEILYFDAGVTGSMARLRAGNPPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4R0</accession>
    <name>U5W4R0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mov34/MPN/PAD-1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560334.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28680"/>
    <dbReference type="EnsemblBacteria" id="AGZ43997">
        <property type="protein sequence ID" value="AGZ43997"/>
        <property type="gene ID" value="AFR_28680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5814"/>
    <dbReference type="eggNOG" id="COG4249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_426212_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="SSF52129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="642" mass="68736" checksum="E6BDD164048B867E" modified="2014-01-22" version="1">MTGIDRRRSRLVLAGIPRYDDEALADVPQAAQNVADLRGVLTDPEIGGFAEEHCLVVPADVNRSGLARAVKRASEEAEDLLIFYFSGHGLLDDHNLHLGLQDSTFEDPGIESIPFDLIRRICLRSSARNKVVILDCCFGGRALGSPLAVSDEVVLADVEISGTYTLTAAPGNKAAVVLPGEVNTAFTGRLLRLLHEGVPGPMKTLTMGMIFRHLDRKSRAEGLPLPQQRNIGMADQIGLVANVAYAPVSHPLATAETGEVPVPVELPHVDPSPARAETLAERVRHGQAASVAADLRAIAEYRPDDQKLWHGRLEQAERAVDLLGAIVATATDGPQLAAEVITEVIAGDRPPSLLLAGLVRGANDSPVARALALVPPETARDLLVYLTLEKYGESSLQEYLGRLIASDKLAEVLTAAVKTYQYSVPVAGVLGAVRLPDLLRQLLDLGHLEAGIVLTWALAPNDLSDDELKNSRTAKTLALACQTLSGTRLARMSRVVALQLRTQPKSAAMLLYHLDRHAPEAGTLLLVRAVDGDNRLGEHLGRLARGTLPLPMAIIARITEHEPATADLLASALVFTDPCSAKKFLDVSLTSQFFFTPPGAPAFGRLIAEKFEVAAHASGWDEVRAAIETGNLELARSLAQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4W2</accession>
    <name>U5W4W2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA repair protein RecN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Recombination protein N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_29935</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">May be involved in recombinational repair of damaged DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the RecN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560585.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29935"/>
    <dbReference type="EnsemblBacteria" id="AGZ44248">
        <property type="protein sequence ID" value="AGZ44248"/>
        <property type="gene ID" value="AFR_29935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6063"/>
    <dbReference type="eggNOG" id="COG0497">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018297_3_0_11"/>
    <dbReference type="OrthoDB" id="1074831at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004604">
        <property type="entry name" value="DNA_recomb/repair_RecN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11059">
        <property type="entry name" value="PTHR11059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003128">
        <property type="entry name" value="RecN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00634">
        <property type="entry name" value="recN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC_N" evidence="8">
        <location>
            <begin position="2"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR003128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="585" mass="61928" checksum="FB887C52463D63FF" modified="2014-01-22" version="1">MLEELRITGLGVIDDTTLRLTTGMNAITGETGAGKTMVVTGLGLLFGGRADAGRVRADPGRAVVEGRLRLEGTLGDAVQTRITDAGGEMDDDGSVLLSRTVTIEGRSRAHVGGRSMPVAMLGEVGEQVVAVHGQSDQLRLLRPSEQRAALDRFAGPEHEKLLETYRESFARWRAVVEDLADRRRHARERNQEADLLTLGLDEISRVDPQPGEDDDLRAEVQRLEHAEGLRTAAALAAQALAGGEEATEETPDATSLLGTARRTLEAQATVDPALGELAARVEEAATLVGDVSAELSAYLSALDADPARLEVIYERRAALRALTRKYADDVEGVIAWAENARTRLAALDNSDELLEELDKERVRLAASVGELAGRLTAARVEAAGRFAGQVSVELAGLAMPHARVEIAVQPRVPGRDEPTVTVDGSEVAVGPDGGDDIELRLIAHPGAPALPLQKGASGGELSRVMLAIEVVFAGAGGPPTLVFDEVDAGVGGQAAVEIGRRLARLARTHQVLVVTHLPQVAAFADRHLVVAKDTGGAITTSGVRIVEETERARELARMLAGLPDSDLGIAHAEELLAVADREKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W553</accession>
    <name>U5W553_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_29405</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29405"/>
    <dbReference type="EnsemblBacteria" id="AGZ44142">
        <property type="protein sequence ID" value="AGZ44142"/>
        <property type="gene ID" value="AFR_29405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5960"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="22"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="295"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="389" mass="40201" checksum="0F5446E80AB42D41" modified="2014-01-22" version="1">MTPLDLLEVGLLGLRTRKTRAALSALGIAIGIATMIVVTGIPASSQAALLRELTALGTNTLQAVATPDQNPPVLLPEESVGMVARIGPVTRVSAVANTHTLVRRSDRTDPQDGSGLTVLAARLDLPSAINARVRTGQWLSAATERFPTVVLGSVAAARLGVDDTRGGPQVLIARTWFTVVGILATTPLLPDIDRSVLVGWPAATDFLQFDGHPTVLYVQAFEPSVEAVRAVLPETIHPEQPGQVQVSRPSDALAAKRATENTFSALFLGLAAVALLVGGIGVANTMVISVLERRPEIGLRRALGASRGQIRGQFLTESVALSTLGGVAGTVLGLVATAGYAAYHGWPVVVPLSSTVAGLAGAVLVGVVAGVYPSMRAARLTPTEALATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5E3</accession>
    <name>U5W5E3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative sugar ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_30865</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560768.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30865"/>
    <dbReference type="EnsemblBacteria" id="AGZ44433">
        <property type="protein sequence ID" value="AGZ44433"/>
        <property type="gene ID" value="AFR_30865"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30865"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6248"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_11"/>
    <dbReference type="OrthoDB" id="1510342at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="67"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ44433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="30742" checksum="89E95789B8261980" modified="2014-01-22" version="1">MRPGRRLLDAFAVLIALATIFPIYWMVLAAVKPPGEIQSLDQRPWTLAPTLDAFRRVVTVDNFGRYFLNSVLVALVVVVLSGLCAFLAAVALTRFRFRLRTTVLVLFLVAQMVPVEALTIPLFFLVRDLGAQVPGIGLNTLGSLMLVHTAFTLPFAIWMLRGFVAAVPDELDEAAQLDGAGRFTILWRILFPLVAPGLAATSVFSFITAWNDFLFARTFIISATDRQTLPMALLVFFKPDENDWGAIMAASTLMTVPVLIFFLLVRRRLVAGLGGAVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5G1</accession>
    <name>U5W5G1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560783.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30940"/>
    <dbReference type="EnsemblBacteria" id="AGZ44448">
        <property type="protein sequence ID" value="AGZ44448"/>
        <property type="gene ID" value="AFR_30940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6263"/>
    <dbReference type="HOGENOM" id="CLU_195022_0_0_11"/>
    <dbReference type="OrthoDB" id="1983596at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="85" mass="9294" checksum="75544275FE8AE0D2" modified="2014-01-22" version="1">MISTVQDLAAEALFVSHLQPSECPSREMVEQTVTAMLLRHGSDGCAAGVAEEFGDHPEAAVRRMGWVRRELGSVMTAPRRPHYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5Y6</accession>
    <name>U5W5Y6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31395"/>
    <dbReference type="EnsemblBacteria" id="AGZ44539">
        <property type="protein sequence ID" value="AGZ44539"/>
        <property type="gene ID" value="AFR_31395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6354"/>
    <dbReference type="eggNOG" id="COG3324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069623_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="10"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="127"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="27016" checksum="1427D950C7ECD885" modified="2014-01-22" version="1">MRIRGFAPATPCWVELASADPARAADFYAGLFGWEPAGDRFKLGGRAAAGLTRARPGRPAGWLTYLTEHNLESSITRVHEAGGRLVSAPADARGGRSALIADPAGALLGLWESDGFTGAQIGGEPGTMTFPELLTDDAVAAASFYGRAFGWLLRDEYGSDGTRGEWITTAHDAMAGLAPSRGGNWWRAAFQVADCAETISTCRSLGGGVIAGPTDMGFGSYAELLDPWGVPFAVAAPAALPVELSMSFTPAAGMELTFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6E3</accession>
    <name>U5W6E3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_32280</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561046.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32280"/>
    <dbReference type="EnsemblBacteria" id="AGZ44714">
        <property type="protein sequence ID" value="AGZ44714"/>
        <property type="gene ID" value="AFR_32280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32280"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6534"/>
    <dbReference type="eggNOG" id="COG4779">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_1_1_11"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="343" mass="35128" checksum="DCC9B522817FC397" modified="2014-01-22" version="1">MSRRITVRGAGLALRLDLRTIWVSGALLLATAAVVTLTVVAAGTRLPIADIIEILNGGGRRADRFILLDLRLPRVTLGLLAGAALALSGAVFQSLSRNPLGSPDIVGFTTGSATGAVLAIVVFGAGPAGAAGAAIVAGILTALIVYGLASIGGGAVRRIVLVGIGISAMLVAVNSYLISRARLDAAQAAAIWLVGTLNGRTWEYVRLLGLALLILAPLLLLLSRRLQMLEMGDESAQSLGVRVNRSRLLLVILAVAVCAVATAATGPVAFVALAAPQITRRLTGSPALQLLPTAITGALLMVLSDYVAQRLLAPAQLPVGVVTGAVGGVYLAWLLSLQWRKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7B5</accession>
    <name>U5W7B5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_34000</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561395.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34000"/>
    <dbReference type="EnsemblBacteria" id="AGZ45058">
        <property type="protein sequence ID" value="AGZ45058"/>
        <property type="gene ID" value="AFR_34000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6882"/>
    <dbReference type="eggNOG" id="COG2875">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_1_11"/>
    <dbReference type="OrthoDB" id="1185397at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="7">
        <location>
            <begin position="3"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="25492" checksum="DC0D6E24BBCF027A" modified="2014-01-22" version="1">MTVHFIGAGPGAADLITVRGQRLIGSSPVCLYAGSLVPAELLTFCPPGARLVDTAGLDLDQILAEMVTAEKAGHDVARLHSGDPSVFSAVAEQMRRLDEAGIGYDVTPGVPAFAAAAASLGREFTVPGVAQTVILTRTAERATAMPPGEDLATLGASRSTMVLHLAVQRIDAVVAELVPNYGVDCPVAVVARASREDELIVRGTLADIAAKVTAAGIKRTAVIVVGAALTATAFPDSHLYSTERCRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7G5</accession>
    <name>U5W7G5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28175"/>
    <dbReference type="EnsemblBacteria" id="AGZ43896">
        <property type="protein sequence ID" value="AGZ43896"/>
        <property type="gene ID" value="AFR_28175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5711"/>
    <dbReference type="eggNOG" id="COG0699">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017620_0_0_11"/>
    <dbReference type="OrthoDB" id="728775at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="722" mass="79080" checksum="D47763E9D7336E4F" modified="2014-01-22" version="1">MNFEIERWERVQQSLGRLGGALSAVREAPDESLAALGLDTVDVTALERRAAEVLAALATVRARVSRHTMNIGVSGRARNGKSTLLQSLTGLGDDQIPAGAGAPVTAVRSRIFHSTERHEARLTMHTEESFCSEVLAGYHEELRLGPPPRTIGEFAGQAYPASKDDLALEPAEQTRLVPMLVRLRVIQSSLDSYRGLLDGRTRNVEFGELRRWVSYPAEGEDRPDRRYLAVRDARITCRFPVVEISDLGLVDLPGLGETVPNAEKHHIAGLENDVDFVLNVKRPDGTNAYWDTHDSAGLGLICQAAGAAPVRDFAAILVNNGALPQANVDALLTAVRENVNENGADAAFPVIVADVKKPETVQAEVLGPVLDHLASALPRMDAAVVDHAVRLSVAAREQLLAVAGAMLASLRSVLVPTAVDELIARSGELRAQTAVAIQEWVADLEQRADEAYEDKEFYDRADQLQSTVHNWILDGFGETTEVWRKRAVARMRVDNASAPFVVNELNGIRVEVARRFSAIDDVLERRREEFWGGLAAALGARLVPFTEGDDPHRALTDLAVRLREAPDPCPVLAEAIDFVLDVRLDYRTRVLPRMRTALMMLRAESSGVPGDTPVAHTDDGAQQLFVHISNLARQANYGAAQTLKEEPATTAQVLLAFAGQFEDQFIRSSASEAEFRRVAEAFRDQLWPDELNGPALATARVQHLRSVLNDLIAHLNDQRSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7T0</accession>
    <name>U5W7T0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_34665</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561526.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34665"/>
    <dbReference type="EnsemblBacteria" id="AGZ45189">
        <property type="protein sequence ID" value="AGZ45189"/>
        <property type="gene ID" value="AFR_34665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7013"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="152"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="224" mass="23277" checksum="29D6407F849313B9" modified="2014-01-22" version="1">MIRVLIADDEPMIRAGVRAVLATDPGIEVVAEAVDGHDAVELVRRHRPAVAVLDIRMPGMNGIDAAAEIRGTVPATSVIILTTFGEDDYILQALGHGATGFLIKSGEPEELIRGVRAVAEGAAYLSPKVAARIVAHLAATGAGALAGRRSAARDRVGALTAREREVLAFLGGGLSNSQIARRLQVVEGTVKAHVSSILGRLGVDNRAAAAVVAHEAGIIRDTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8I0</accession>
    <name>U5W8I0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_30125</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560622.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30125"/>
    <dbReference type="EnsemblBacteria" id="AGZ44285">
        <property type="protein sequence ID" value="AGZ44285"/>
        <property type="gene ID" value="AFR_30125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6102"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="148"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="217" mass="24225" checksum="5BF82F2ABB9CC348" modified="2014-01-22" version="1">MTAPTRILLADDHALVRRGVRLILDQESDLTVIGEAADGAEAVELARADPPDLAILDIAMPRMTGLQAARELNRRLPALRILFLTMYDNEQYFFEALRVGAAGYVLKSAADRDLVEACHAAMRDEPFLYPAAETALVRDYLDRARAGGDIPARAVTDREEEVLKLVAEGHSTREIAELLYISHKTVERHRSNILQKLGLRDRLELTRYAIRAGLIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8I6</accession>
    <name>U5W8I6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35305"/>
    <dbReference type="EnsemblBacteria" id="AGZ45317">
        <property type="protein sequence ID" value="AGZ45317"/>
        <property type="gene ID" value="AFR_35305"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35305"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7143"/>
    <dbReference type="eggNOG" id="ENOG5032TUY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070587_0_0_11"/>
    <dbReference type="OrthoDB" id="1550026at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="29349" checksum="F899A655CA3566D1" modified="2014-01-22" version="1">MSRPDENSAQSADPGRELIKVDPAEPAEEGIGATVAALSADDLEPAKRRHLLGRLVGDVRRRGLGQLFRPKAALRWMADVVADVAPHVPVRDLETLRLHFDGLEGDDLADRLIRNAARATAGIGAAGGGVASLEWAATPTLLSAPVLLAAETVAVVSIELKLVGELHEVYGRPVTGTATQRASSLIQAWAGQRGVNPLVPGVGVATVLGTAARKELQSTLLRRFGRNLTTLGPLLTGAAVASYLNRRATRGLGESLTKDLRKGKKNAAIIDPGRKEISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8M7</accession>
    <name>U5W8M7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_30400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560677.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30400"/>
    <dbReference type="EnsemblBacteria" id="AGZ44340">
        <property type="protein sequence ID" value="AGZ44340"/>
        <property type="gene ID" value="AFR_30400"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30400"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6155"/>
    <dbReference type="eggNOG" id="ENOG502ZAIH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071247_0_0_11"/>
    <dbReference type="OrthoDB" id="1906975at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="35818" checksum="903BD624ACB5C64B" modified="2014-01-22" version="1">MRLAKTAEFGLPDKVGGPIRAGSCLVSGAGVFSAVVWWDGGARYALVSIADQVVRTMVLDELGDPFDAYDAPAPVLLDDGSFGVIADAGTLRLYGTDLRVQREMAIAGDEVLHRFARHGQRPRVFGHASRGALASEHVVVLDEPIATGNPRYLAFLRIGETSASWTRIGTLGPDGFPVDRFGDDELNQSPDVSVGGCIIGDAVGHSGSLLVCVEGSDASSVRRYGADFFTITRVTPDGSVTEPLYQQSGWKRLPGKHAINGKVTSSGRYVLLTPVFASGDWKGRQRLFDLETGEVLEPVLPRGAAKMRIVDHAGTTFWLSDGAQQILCAEAEGPHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Q6</accession>
    <name>U5W8Q6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">smc</name>
        <name evidence="8" type="ORF">AFR_36485</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SMC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561889.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36485"/>
    <dbReference type="EnsemblBacteria" id="AGZ45553">
        <property type="protein sequence ID" value="AGZ45553"/>
        <property type="gene ID" value="AFR_36485"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36485"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7382"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_2_0_11"/>
    <dbReference type="OrthoDB" id="1149850at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="SSF75553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="7">
        <location>
            <begin position="512"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="732"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="955"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="330"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="408"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="661"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="870"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="738"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="960"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ45553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1193" mass="129469" checksum="BE599FEE942BA82E" modified="2014-01-22" version="1">MHLKSLTVKGFKSFASATTLRLEPGITCVVGPNGSGKSNVVDAIAWVLGEQGAKALRGGKMEDVIFAGTAGRAPLGRAEVTLTIDNGDGALPIDYTEVSITRRMFRSGESEYEINGSSCRLLDIQELLSDSGIGREMHIIVGQGRLDAMLHAKPEDRRAFIEEAAGVLKHRKRKEKAIRKLDAMQTNLNRLTDLTAELRRQLKPLGKQAEVARRAAGIQADLRDARLRLLADDLHTLRTTLDKEIADESAMRERREQVEGENREVQRWLADLEASHAEDAPLLAAAQDTWYKLSALQERFRSTEQLASERLRHLAAAPDDERPGRDPDQLVAEAERVREQEEELREALTDDQMRLAEAVEHRQELERQLLQAEGALRAAAKAIADRREGLAKLTGNVNAARARTGSAAEEIERLAVAHNDALMRAEAAQAEVDAVSAESTEADRDNAELDERHAEAVALHDRAAAVVKELSDAERTAEKDAASWKAREEALALGLKRKDGAGALLAKADQVPGLLGSLASMLTVRSGHEAALAAALGALADSVALTGIDEAVEAMRTLKIADAGRAALIVGGPAAPGMQGSLESLRPALPEGATWAPDVIECPDTIRPALNRALRDVVLVPDLAAATRLVADNAELRAVTPDGDVLGAHAAAGGSGKATSYIEVQAAVDEARANRETAEQSIGVLKGQLADARAEVAEHKEAVNVAAAAKRAAEGERNAAARRLAELGAAARSARAESERLGASRQKAEGNRDRDLAGLAELEERLRLAEATPIDEEPSTEERDTLSALVPQARQNEMEVRLAVRTAEERVSSIAGRADSLMRQANQERQARERAAARRAARARGAEIAKAVALGAAGAMSRVAVSLAAAVEVRDELARARTAREAELQEVRASAKRLVSELERLTNEVHRDEVARAEQRMRIETLEAKAAEDFSLDVDTLVAEYGPEQLVPPTQAEAAQAEKDGKELPEPVPFHRPTQEKRANKAERDLALLGKVNPLALEEFAALEERFKFLSDQLEDLKATRKDLLTVVKDVDDRILEVFASAFEDTAREFQTVFQVLFPGGEGRLVLTDPEDMLTTGVEVEARPPGKKIKRLSLLSGGERSLTAVAMLCAIFRARPSPFYIMDEVEAALDDVNLGRLITLFQQLREKSQLLIITHQKRTMEVADALYGVTMRAGVTQVISQRLNREPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8R2</accession>
    <name>U5W8R2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Spherulin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_36510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561894.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36510"/>
    <dbReference type="EnsemblBacteria" id="AGZ45558">
        <property type="protein sequence ID" value="AGZ45558"/>
        <property type="gene ID" value="AFR_36510"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36510"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1438271_0_0_11"/>
    <dbReference type="OrthoDB" id="1974060at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="20276" checksum="BC6466344D59278D" modified="2014-01-22" version="1">MKTLFPPYAQPSPELALDADTWVVQERPGPRRSTHQVLGKVDLDWGGRSLADVLADVDAWREDGAEGLFLDRAPAGSGGVGPVALTVRLAARRGLHRVVLNPGVPTHPLYRELGVRICTFDGPWSAYQGWAGDGVRPGDGHLVHGVPTPLLTAARRLMGRRGAGFGLATDASPALSAVAAGGAAGSADSCRTSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9J4</accession>
    <name>U5W9J4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_38145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38145"/>
    <dbReference type="EnsemblBacteria" id="AGZ45883">
        <property type="protein sequence ID" value="AGZ45883"/>
        <property type="gene ID" value="AFR_38145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7717"/>
    <dbReference type="eggNOG" id="COG1162">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033617_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01854">
        <property type="entry name" value="YjeQ_EngC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004881">
        <property type="entry name" value="Ribosome_biogen_GTPase_RsgA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010914">
        <property type="entry name" value="RsgA_GTPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32120">
        <property type="entry name" value="PTHR32120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03193">
        <property type="entry name" value="RsgA_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00157">
        <property type="entry name" value="TIGR00157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50936">
        <property type="entry name" value="ENGC_GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="4">
        <location>
            <begin position="80"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="ENGC_GTPASE" evidence="3">
        <location>
            <begin position="89"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="312" mass="33289" checksum="98D3181E93599597" modified="2014-01-22" version="1">MITVDRGRYGCIREDAEHLDELITAMRARELGRKSVVVGDRVALVGDTTGAPGALARIVRISERTSVLRRTADDDDTTPEGRLERVVVANADQLVIVSALADPPPRTGFIDRCLVAAYDAGIEPLLCLTKADLDGPAAVLDYYAELDLPYVLVQPDSDLTELRDKLAGNISVMVGHSGVGKSTLVNRLVPDALRAVGVVSAIGKGRHTSTSAVALRLPRVHKKGDPGWIVDTPGIRSFGLAHVSAESLLHGFPDLVEGTVDCPPNCGHTAADVNCALDAWIAAGKADPRRLASYRRLLSSRAGEPDPRDQMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9P6</accession>
    <name>U5W9P6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pre-mRNA-processing ATP-dependent RNA helicase PRP5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_31880</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560969.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31880"/>
    <dbReference type="EnsemblBacteria" id="AGZ44636">
        <property type="protein sequence ID" value="AGZ44636"/>
        <property type="gene ID" value="AFR_31880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6453"/>
    <dbReference type="eggNOG" id="COG0513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_3_11"/>
    <dbReference type="OrthoDB" id="643629at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="9">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="33"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="235"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="385"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="453"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ44636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="507" mass="52696" checksum="3F5E8CD6871B0F82" modified="2014-01-22" version="1">MSSFIDLGVPTKLAAALADLGITSPFPIQAATLPDSLAGRDVLGRGRTGSGKTYAFVLPVLARLAESKAPRRPMRPRALILAPTRELATQIEATLAPLAELLGLRTLTVFGGVGANPQIRGLKAGVDVLVACPGRLADHIQQGYASLDAVQITVLDEADHMADLGFLPVVRRLMDGTPRDGQRMLFSATLDGGVDVLVKRFMHKPVTHHVDSAAEAPIEMAHHVLHVRHDDRFPVLVDLTAAPGRTVVFTRTKRRAKTLTRQLVQAGVPAVELHGNLAQNARNRNMAAFSDGTAQTLVATDIAARGIHVDDVAIVIHADPPVEHKAYLHRSGRTARAGAQGTVITLMTDDQQSEVRDLTRKAGIKPTTTKARPGDALLAQLAPGERTYSAPVSPVPEPETDNAGGRTRRPRPAGTGGGRGAGAARGTGAARGSGAARGAGAPRTGGDQPTRGGQGGGRSQSGHTQGTRSQGGASRGRRGGTGSTAVHTSQSSSGGAAAFSSRSRAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA09</accession>
    <name>U5WA09_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FMN-dependent NADH-azoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561153.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32785"/>
    <dbReference type="EnsemblBacteria" id="AGZ44815">
        <property type="protein sequence ID" value="AGZ44815"/>
        <property type="gene ID" value="AFR_32785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6635"/>
    <dbReference type="eggNOG" id="COG0431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_3_2_11"/>
    <dbReference type="OrthoDB" id="796211at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="4"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="154" mass="14730" checksum="3299FB2970BE2780" modified="2014-01-22" version="1">MSGIVVVSGNPRAGSRTSTLAAAVGSALGGASTVIEVGALGTGLLTPDDPATAAAVTAVREADVLVVATPTYKGSYTGVLKVLLDQLPANALAGKRAVPVVTAGVAPQAAAAAALLAQLLGELGADVAPALPVVEADLPESAAIAEKYAAAHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA47</accession>
    <name>U5WA47_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">AMP-dependent synthetase and ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562377.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38945"/>
    <dbReference type="EnsemblBacteria" id="AGZ46043">
        <property type="protein sequence ID" value="AGZ46043"/>
        <property type="gene ID" value="AFR_38945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7882"/>
    <dbReference type="eggNOG" id="COG1541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035301_5_2_11"/>
    <dbReference type="OrthoDB" id="839891at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="425" mass="48600" checksum="D1A9A7E34407C859" modified="2014-01-22" version="1">MFLREALYVPVTLIRQYAPAGTLERRKLRWINEMLAFAAERVPFYRDYDLYSRHPLQDLSELTTLPVLRKETLRNRPQEDFLAEGTDRSGWRDFQTSGSTGRRVLVRHDGRSHDYHLAACFRRFLATGRYLPTYRLSHLRPYEGPPRFFEKFKLFRRHTVLTRRPMDEIKAELLGNRPQVLIGWPVHLRELLRSLSAGELAQLRRTLKLVFTESELLTPQLRAQFTESFGVPVFDEYSAFEVLNVYFECSRGGRHISEDRVHVEVVDDDGRPLPEGAEGRLLVTAYMERAMPLVRYAIGDIGRINPDRCPCGRTFRTLELTRGRADDVVVLADGRKLYADTFMWISAWHPGVAECFVHQDAAGRVRMSVVLDPEADPEPVLDAVRAELFVLANGPFELELVIAEALPVSAGGKGKLVASEYLAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WA49</accession>
    <name>U5WA49_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ccyl-CoA dehydrogenase type 2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_32765</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32765"/>
    <dbReference type="EnsemblBacteria" id="AGZ44811">
        <property type="protein sequence ID" value="AGZ44811"/>
        <property type="gene ID" value="AFR_32765"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32765"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6631"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013107">
        <property type="entry name" value="Acyl-CoA_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08028">
        <property type="entry name" value="Acyl-CoA_dh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="2">
        <location>
            <begin position="108"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_2" evidence="3">
        <location>
            <begin position="227"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="369" mass="38835" checksum="F91EF489E0CAA0E8" modified="2014-01-22" version="1">MDVTAALAEHAEEHDRTADLPEKGLAIVHTAGLLTLTVGTRHGGPGGGLHDTVRTLTALGQGDPSVALISAMTLFAHAAAASWPADVYAEVLAESARRPTLINALRVEPDLGTPARGGLPATTARRTTDGWELTGHKIFSTGATGLRWMQVWARTDEDPVRTGAFLVRTDSPGITVERTWDHLGLRASRSDDVTFDRVKVPAGATLDLAAPGPPGADPGGLRAWNALGIAAIYLGVARAARDWLVGYLQDRVPTNLGKPLASLPRFQNEVGVIAAQLLAATSLVETTALAVDRGAVLDPAQPNIAKSVATNAAIDSVQRAVALIGNAGLTRHNPLERHLRDVLCGRIHTPQDDVVLASAGRTLLERNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAE4</accession>
    <name>U5WAE4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdenum ABC transporter periplasmic substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein ModA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561253.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33290"/>
    <dbReference type="EnsemblBacteria" id="AGZ44916">
        <property type="protein sequence ID" value="AGZ44916"/>
        <property type="gene ID" value="AFR_33290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6737"/>
    <dbReference type="eggNOG" id="COG0725">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065520_0_1_11"/>
    <dbReference type="OrthoDB" id="1506401at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005950">
        <property type="entry name" value="ModA"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004846">
        <property type="entry name" value="ModA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01256">
        <property type="entry name" value="modA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665859" evidence="4">
        <location>
            <begin position="25"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004846-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="26446" checksum="8EEDB01603F640DB" modified="2014-01-22" version="1">MKARLLAAVALAAALSMTGCGGSALNDDSPAGGGSSASGPAGSSAAGPITVLAAASLTESFDRIGTLFEQINPNLDITVSYGGSSGLATQITSGAPADVFAAASPATMATVTDAGDTTGTPATFARNQLVIAVPKGNPKGLTSLGKLAGSGIKVALCAEQVPCGAAAKKALDAAGVKLTPVTQDQDVKAALAKLKLGEVDAALVYRTDARSATADVDGVEFPESAEATNDYPIVALKDAPNPAGAAAFVAFVQTPEAQQVLTDAGFQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAM7</accession>
    <name>U5WAM7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Magnesium transporter mgtE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_40045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40045"/>
    <dbReference type="EnsemblBacteria" id="AGZ46263">
        <property type="protein sequence ID" value="AGZ46263"/>
        <property type="gene ID" value="AFR_40045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8104"/>
    <dbReference type="eggNOG" id="COG2239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030870_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006668">
        <property type="entry name" value="Mg_transptr_MgtE_intracell_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038076">
        <property type="entry name" value="MgtE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006669">
        <property type="entry name" value="MgtE_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43773">
        <property type="entry name" value="PTHR43773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03448">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00924">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="286"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="355"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="429" mass="47493" checksum="0FC45CB6C6D29DE8" modified="2014-01-22" version="1">MGSRVYLARLAALPVFDPNGDRVGRVRDAVIRLRTTNRPPQVVGLVAEMALRRRIFLPIGRFTSMDAESVVLSTGMLNLRRFEKRPNELLVLEEVLDRRVTVEPEDRQGPGQPGSVVDLGMELNRNNEWLITRVAVREHSGRLTRRGHTYQVEYDRVRGLVGPTDTQGTSNLVALLEQMRPADMANALQDLSDSRRNEVAHALSDRTLADVLEELPEHDQVEILVQLDRERAADVLERMDPDDAADLLAELPKAEQAVLLDLMEPEEAAPVRQLMSYTPGTAGSVMTSEPVILPPDATVAEALARIREPELSPVVAAQVFVARAPSATPSGKYLGMVHFQRLLREPPSSILGGIIETDLDPLSPETDLTEITRRMATYDMVAMPVVDSAYRLVGAVTVDDVLDHSLPRDWRDRDIQDETPTPILPGASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAR1</accession>
    <name>U5WAR1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RibD_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562562.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39875"/>
    <dbReference type="EnsemblBacteria" id="AGZ46229">
        <property type="protein sequence ID" value="AGZ46229"/>
        <property type="gene ID" value="AFR_39875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8068"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_4_0_11"/>
    <dbReference type="OrthoDB" id="1306955at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="2"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="190" mass="20674" checksum="EBC4EFCA916680A9" modified="2014-01-22" version="1">MRKLTYYIATSIDGFIAGPGGEFDFFGFEGDFAAAILEEFPETLPAAAREPLGLIDVANKRFDTVLMGRGTYEPGLAVGMTSPYSQLRQYVFSRTLTSDAPDVEIVGGDPVGFVRKLKQEDGLGIWLCGGGKLAAELRDEIDELIVKINPLVLGAGIPLFAGDFRPDQLRLVESRTFETGTIIATYARRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB34</accession>
    <name>U5WB34_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34440"/>
    <dbReference type="EnsemblBacteria" id="AGZ45146">
        <property type="protein sequence ID" value="AGZ45146"/>
        <property type="gene ID" value="AFR_34440"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34440"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6968"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013835_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="743"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="795"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="844"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="883" mass="91725" checksum="BD5EBBD01F04BCB3" modified="2014-01-22" version="1">MRRLRAYAGHWALLLILTLVAGLLAAGLPRLANEFTDRGLQGDVTRLPHTVRDVSYRAAPELKTQPSTALAQLPTVESRLPAPLRALVEDRWASGQVGPLGLSATGPGMFAQACTPQPQIRTQTGTEQAVRLAEGRLPRSGQMVETVLSRDAATTAQLRVGDTLTVAGATGTVLVRVVGVFDPVDAAAPFWDDQRLESVTCPRPDEGMTFRLTLLTDAAGLKLATRTAPAAYAWRFRINAQRLRASDIPELTAALVAARRTPPLPGTLLETGLDGTLTTFDQKLRGARATLAIVQSGLLSTLAGLVVLAALLLVDRRRQEFALLRARGSTATRVGLRTLAETLLVAPAGVLGGWAIALLLPGRPDAGENVLLLALLFFATLTGPVLAARARSAPGRARLRPSARRITAEVFLVVVAVLGVVLVRRRGLSGTVDPYLVLVPVLLAAGAALVAVRLLPWPLRQIGRLAARARGALPFLSLARAGRGAAVTIGPLAVLVVAIATGVFTGAVSETVSGARDQATDQEIAADAEVTGFGFAPSTTGLLTAVPGVTAAAPMLIGSGVPLTAASGRRTQAQLLIVDGAAADEVMSRSGVGVRLPSALSATASGGPVPVVVSPDVAAEVGAGGVIVVQGQRYDFRVAAVQSAMPGLGVSVRRFVAVPWRALPVPASQPLVPTRYLVAAGSFDKAALLRAADTGQREYYLGLLQRTINADATAVSDAQLPRRATATTWQEYRRSLEDSGANAVLSFTFTAGAAGAAALALLAVGLTVLADAPGRGRTLSRLRTMGLSLRQGRRLLIYELVPLIGVALVAGGLVGVFLPRLIGPALGLDAFTAGLPAGTRVGPWLPAAALLLVAAALATAVVVENLINRRMRLGDVLRLGEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBF3</accession>
    <name>U5WBF3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">D-inositol-3-phosphate glycosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.250</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferase</fullName>
            <shortName evidence="7">GlcNAc-Ins-P N-acetylglucosaminyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mshA</name>
        <name evidence="10" type="ORF">AFR_40315</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>1D-myo-inositol 3-phosphate + UDP-N-acetyl-alpha-D-glucosamine = 1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside 3-phosphate + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:26188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:58892"/>
            <dbReference type="EC" id="2.4.1.250"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyltransferase group 1 family. MshA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.250" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562649.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40315"/>
    <dbReference type="EnsemblBacteria" id="AGZ46317">
        <property type="protein sequence ID" value="AGZ46317"/>
        <property type="gene ID" value="AFR_40315"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40315"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8159"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_3_11"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102710">
        <property type="term" value="F:D-inositol-3-phosphate glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010125">
        <property type="term" value="P:mycothiol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01695">
        <property type="entry name" value="MshA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017814">
        <property type="entry name" value="Mycothiol_biosynthesis_MshA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03449">
        <property type="entry name" value="mycothiol_MshA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="9">
        <location>
            <begin position="34"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="8">
        <location>
            <begin position="225"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-GlcNAc binding" evidence="7">
        <location>
            <begin position="28"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="1D-inositol 3-phosphate binding" evidence="7">
        <location>
            <begin position="33"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ46317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="434" mass="46642" checksum="F5F03CF95948C0C0" modified="2014-01-22" version="1">MAELRASGLWPTPRRIATLSVHTSPLEQPGTGDAGGMNVYIVEVSKRLAERDVEVEIFTRATSSELPPVVEMAPGVTVRHITAGPFEGLSKEELPSQLCAFTNGVLRAEAARPPGAYDLIHSHYWMSGQVGWLARERWGVPHVHTAHTLAKVKNRFIAAGDRPEPKARVIGEEQVITEADRLVANTRFEAQDLVSCYDADPTKLTVVQPGVDLGRFRPRSTRVADRRRLGLPERGYVVAFVGRIQPLKGPDVLIRALASEPLRDEDVTVVICGGPSGSGLDRPTSLIELASSLGVSHKVHFLAPQTGEALAALYRSADLVAVPSHNESFGLVALEAQACGTPVVAAAVGGLVTAVTDGVSGVLVDGHDPQDWARVLTGLLLAPAQRLRLSIGAVRHAKNFSWDRTADGLLRVYREAVAGHRALIEARLAGSFSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBH4</accession>
    <name>U5WBH4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_41225</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41225"/>
    <dbReference type="EnsemblBacteria" id="AGZ46499">
        <property type="protein sequence ID" value="AGZ46499"/>
        <property type="gene ID" value="AFR_41225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8343"/>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037014_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016977">
        <property type="term" value="F:chitosanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00978">
        <property type="entry name" value="chitosanase_GH46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.386.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000400">
        <property type="entry name" value="Glyco_hydro_46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023099">
        <property type="entry name" value="Glyco_hydro_46_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01374">
        <property type="entry name" value="Glyco_hydro_46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665893" evidence="2">
        <location>
            <begin position="35"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="3">
        <location>
            <begin position="45"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="411" mass="43295" checksum="8C61A48AF09CFCE4" modified="2014-01-22" version="1">MTIPRLRKRTIAYALGGVLVPAAAVAYGLMPASAATAGPIKGLGGKCVDIAAASSANGTAVQLYDCNSTGAQNWTVGNTDNSIKALGKCLDVAAAGTANGTKIQIYDCNGTAAQKWTPSNGTLVNTGSGKCLDATGNSSANGNQLQIWSCGTGANQKWTLPGGGTTPTTSPTTTAPPSGGVNLDNPAKKDVAMQIVSAAENSSLNWRAQFSYIEDIGDGRGYTAGIIGFCSGTGDMLELVQAYTATKPGNVLAKYLPALRNVNNSDSHAGLDPNYTRDWKTAAADPVFQAAQESERDRVYFNPSVRDGKSDGVRALGQFAYYDASVVHGYEGMRSIRSRALQRAKPPSQGGNETTWLNAFLDERVIEMKKEAAHDDVTRIDTAQRVFLRNGNLDMNTPLDFSIYGDSYHIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBT1</accession>
    <name>U5WBT1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfate transporter/antisigma-factor antagonist</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36020"/>
    <dbReference type="EnsemblBacteria" id="AGZ45460">
        <property type="protein sequence ID" value="AGZ45460"/>
        <property type="gene ID" value="AFR_36020"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36020"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7291"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_11_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="1">
        <location>
            <begin position="10"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="107" mass="11165" checksum="9450FC19FCEB3DD6" modified="2014-01-22" version="1">MDQDAAGTDFSASASVEGNRATVVVTGEVDMSTADAMYRAAAGDTAGADGVTLDLRGVTFFDSAAIQALVRLAEHFTGRLEVVPSAQVHRVLDIAGLTGQPWLLKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBU8</accession>
    <name>U5WBU8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heat shock protein 70</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_41090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562804.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41090"/>
    <dbReference type="EnsemblBacteria" id="AGZ46472">
        <property type="protein sequence ID" value="AGZ46472"/>
        <property type="gene ID" value="AFR_41090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8317"/>
    <dbReference type="eggNOG" id="COG0443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_303999_0_0_11"/>
    <dbReference type="OrthoDB" id="410781at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="514"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="385"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="960" mass="99419" checksum="86B05D068862B1FE" modified="2014-01-22" version="1">MTDGYVLGVDLGTSHTVAMLRWPDGRTRPLLFDGQPLLPSAVYLDTTGRLHVGRDALRLGQAEPDRFEPNPKRHIDAETVLLGGSDVPTADLLAALLGAVAREAVAATGFLPPAVLTYPAAWGSRRREVLTTALARAGWPPSTRLVAEPVSAARYFADVLRRPVPVGSALAVFDFGGGTLDIAVVRNEGVTPEGLPTFAVAASGGVDDLGGLDLDAALVDHLGATLEKAEPAAWAALTEPVTLAQWRARRQFWDDVRGAKEMLSRTPQAPVPVPGVERAVHLTRDEFETAAGPLLRRGVEEAGAVITAAGLQPADLAGLFLVGGSSRVPLVARLLHSELGIAPTVLEQPELPVAEGSIIAGSAPATDAGETPAQPLRAAAVPPTTVTMAHGGPTATAATNGTAPTSGRPVKTEAQPVAVNAAPASAAPAPAPPAAPAGQAQVGESDYAEPVDPWATGEAAAFGNGGGPVLHPVSGAPVSGSPVSAPPPAEPWLASTHEEANANTRVKAYRRKGLWVVAAATVVVLGAAATATVLLWPGYPALDYQALSEEHRVAPAVPVTSAFSGTAVRDKKAYFASADDNGTLGVVAADTESGKKAWSSTDAGAATRWEFFFALPDAVVAISDTDSGTSTRRMVLLDPDDGKKMWDRTVASADNVLFAGDKVVLVDRTENRLVGLEIGEQGKVGWELKSPKSEYGLGTTQVVAATTEDDLTGPATATGLPFAEVFDDDARVVQIGADRSARVVDASTGKVVAGPRQNVGEPDDPIVAHNGRLIVAESGDSHRVVAYDLAKLAETRVVYTAPKSDTQFGHLTACGADRICFAERTGYDAKTAQVVAVDAAKEGELWRRAVPEVDSLVPVGEAVLATRNSSPVQTTLLDAKGKVSWTRGGVIARIDGGNTLQFSKALSTSADDPGLAGEHLGDKAVPLGSLADVRSSTCSWDTTHLACVADEDFVIQRFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBX8</accession>
    <name>U5WBX8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Stage II sporulation protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_36025</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36025"/>
    <dbReference type="EnsemblBacteria" id="AGZ45461">
        <property type="protein sequence ID" value="AGZ45461"/>
        <property type="gene ID" value="AFR_36025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7292"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_82_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="356"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="624"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1221" mass="131908" checksum="A71F2A5766E4EDCC" modified="2014-01-22" version="1">MKHLLEERMRARPDGALPERLRTAFERGGEMGRRMLELDWAATPLGEPAGWPPELANAVYTMLASKAQIVVFWGPDFCALYNDAYIPTMGSKHPDHLGRPGREMWAETWSLLQELFDDVVARDESFYATDHPFMLERFGFLEETYFDVSYDPIRLVDGSVGGVFCIVSDTTGRVLADRRVRTLSALGSRLADSPDQAALGTQVAQVLGENEADVPFAALYLDDPADGDVLTPAGACGLALDACWPLSAEARQVIETVMRTDSAGSLPVTEAAHPPPSSAADEVLVLPVGGGTSAVGVLVIGVSRVLAMDRGYRDFLDLVIAQISRAVANLRAYEQERRRAAELAALDTAKTNFFSNVSHEFRTPLTLILGPLEELLASPGLGDDQRERLLPMHRNGLRLLKLVNTVLDFSRLESGRLQAAYRPTDLADYSARLASTFRSAAERAGLQLVVDCPPLSAPVYIDREMWEKVVFNLLSNAVKFTPTGRITVRIGERHGSAVLEVSDTGVGVSADEQRLLFDRFHRVTGAWSRSHEGTGIGLALVRELTELHGGTATAQSEVGVGTTFTVSIPMGTAHLPADRIVDEATTALPDDQTRLYVEEAHWWLGETTPAQVAPGEVQEARRGRVLLADDNADLRDHVARLLRPYWDVTAVVDGQEALDRALEEQFDLVLTDVMMPRLDGFGLIKALRSDERTRDVPIVVLSARAGEEASVEGLSAGADDYLVKPFSARDLSARVRANLELGQLRRQIVSRLRGLVDAAAAVNTVRTTAEVLDVAARHVRAMTSAGRVVVTTPGARAEVDGGAADSAAPDAVLPLPDTSGAPLGELRVWAGEDGPPEVGFLTQLARLIGLRLENARLYEAEHRIASTLQHSLLPQTLPRVPGAIVASRYLPGSSEAEVGGDWYDAIVAPGGQLVLVIGDVVGKGVQAAAGMGQLRNALRAYILEGFDCGEALTRLNRLVDNLGRRQFATVVCVRFDPRTGQLFFSSAGHPSPIVASPGEEGDLLYSSALGPPIGALSESVYPTRESQLTPGQRLLLYTDGLIEDRRKGIDTGLAEVRADLGKPNDHVEDLLDTLLAKAAQQTRRDDIALLALQATEPREFVLRLPADPTRLSVLRRRLEDFLTAHDVPENDIFDLTVAVSEAAANAIEHPVEPAEPTITVEVSLDDEAVLATVRDTGSWRPSTGAGFRGRGLALIGALSELSVARSEAGTAVTLRRPISRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBY3</accession>
    <name>U5WBY3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Betaine aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_36050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561802.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36050"/>
    <dbReference type="EnsemblBacteria" id="AGZ45466">
        <property type="protein sequence ID" value="AGZ45466"/>
        <property type="gene ID" value="AFR_36050"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36050"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7297"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_11"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="4"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ45466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="453" mass="48025" checksum="5C9B1B9435986E38" modified="2014-01-22" version="1">MDSTDVINPATGTVLGTVPSNGLAETDAAIERAQRAFESWRQVAPGDRARLLRRFAAVVGDHIEELAGLEVRNSGHTIGNARWEAGNVRDVLDFYAGAPERLSGRQIPVAGGLDVTFHEPLGVVGIIVPWNFPMPIAGWGFAPALAAGNTVVLKPAELTPLTAIRLGELALEAGLPEGVFTVIPGKGSVVGQRFVTHPAVRKVCFTGSTPVGKSIMAGCADQVKRVTLELGGKSANIVFADADLERAAAAAPAGVFDNAGQDCCARSRLLVQDTVYERFLSLLEPAVKAFRVQDPSLDTAEMGPLISAAHREKVASYTGGAEVAFAGEAPKGDGWWFPPTVLLAHSADDAHWREEVFGPVVSVLPFHDEADAVRLANDTEYGLSGSIWTRDLGRALRVSRGVETGNLSVNSHSSVRYWTPFGGMKQSGLGRELGPDALLSFTDVKNVFIATDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC15</accession>
    <name>U5WC15_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pantothenate synthetase</fullName>
            <shortName evidence="5">PS</shortName>
            <ecNumber evidence="5">6.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Pantoate--beta-alanine ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Pantoate-activating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">panC</name>
        <name evidence="6" type="ORF">AFR_41390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(R)-pantoate + ATP + beta-alanine = (R)-pantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">The reaction proceeds by a bi uni uni bi ping pong mechanism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the pantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.1" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562864.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41390"/>
    <dbReference type="EnsemblBacteria" id="AGZ46532">
        <property type="protein sequence ID" value="AGZ46532"/>
        <property type="gene ID" value="AFR_41390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8376"/>
    <dbReference type="eggNOG" id="COG0414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047148_0_2_11"/>
    <dbReference type="OrthoDB" id="1661843at2"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004592">
        <property type="term" value="F:pantoate-beta-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00560">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00158">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003721">
        <property type="entry name" value="Pantoate_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042176">
        <property type="entry name" value="Pantoate_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21299:SF1">
        <property type="entry name" value="PTHR21299:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02569">
        <property type="entry name" value="Pantoate_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00018">
        <property type="entry name" value="panC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>(R)-pantoate</name>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="183"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="277" mass="29550" checksum="30382BAF0D7AC2F8" modified="2014-01-22" version="1">MTALVHTRAELAAARSAATGRVAVVMTMGALHEGHRQLMREARERGDFVLVTIFVNPLQFGPNEDFDKYPRTLDADVEACRAEQVDLVFAPDRDEMYPGGSPLVTLAAGPMGEILEGASRPGHFSGMLTVVAKLLLLTRADLAFFGEKDFQQLSLIRRMVRDLEIGAEIIGVPTVREADGLALSSRNRYLSAEQRQAALALSQALREGAAQSDAESALEAAGKILADEPGVQVDYLALTDPLLGPAPADGPARLLVAAKVGTTRLIDNVPLLLGEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCS3</accession>
    <name>U5WCS3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP synthase F(0) sector subunit c</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">F-type ATPase subunit c</fullName>
            <shortName evidence="11">F-ATPase subunit c</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">atpE</name>
        <name evidence="13" type="ORF">AFR_37360</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Key component of the F(0) channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562061.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37360"/>
    <dbReference type="EnsemblBacteria" id="AGZ45726">
        <property type="protein sequence ID" value="AGZ45726"/>
        <property type="gene ID" value="AFR_37360"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37360"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7558"/>
    <dbReference type="eggNOG" id="COG0636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_5_2_11"/>
    <dbReference type="OrthoDB" id="2078221at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="12">
        <location>
            <begin position="12"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="site" description="Reversibly protonated during proton transport" evidence="11">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ45726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="75" mass="7720" checksum="EC71C1B208E1941A" modified="2014-01-22" version="1">MELFAAVEGSTAAIGYGLAAIGPGIGVGLVFSAYIQSTARQPESSRLTLPYVWIGFAVIEALALLGIAFGFIWAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCS5</accession>
    <name>U5WCS5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37755"/>
    <dbReference type="EnsemblBacteria" id="AGZ45805">
        <property type="protein sequence ID" value="AGZ45805"/>
        <property type="gene ID" value="AFR_37755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7635"/>
    <dbReference type="eggNOG" id="ENOG5033GN1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140382_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="13232" checksum="273983189566ABA4" modified="2014-01-22" version="1">MPVMNGPLSTATIVVALLLGIWYLVRSALDRAPSNTDLWAMLGLSLLVAVLVVVAVVGLITGERPSEWSTFVGYLITTIAFAPVGFYLARLEPTRWGTLILGVACLVLPVLVLRLQQIAEVTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD72</accession>
    <name>U5WD72_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta keto-acyl synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_38235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562236.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38235"/>
    <dbReference type="EnsemblBacteria" id="AGZ45901">
        <property type="protein sequence ID" value="AGZ45901"/>
        <property type="gene ID" value="AFR_38235"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38235"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7735"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_329195_0_0_11"/>
    <dbReference type="OrthoDB" id="872218at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_DH" evidence="2">
        <location>
            <begin position="622"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="872" mass="90037" checksum="3026A9C3DA6D5CC2" modified="2014-01-22" version="1">MPQIPLSETSPPPAADAPGWEVLELARRTAEAHATFQRLMTEAHVEYLRVTETILAGRLAAAPVSGLPAESARISPGPDTAPVSAAQPAIDAPAVRVAPVVPVMPVVPAPAPTTVPAQPDRQGTPPAAPPAAPPAVPQAAAPTAPSAAPPAVPSADEKPESASKPESASKPESASKPEPLFTAEPLLDIPAPPVPPIAVVPEIVPEVPFPEGGVLVRPAPSWVETPLGGAPLPGLDAGPVHVVDGGSGLAAAVAAELTGRGVTAEASDGWGTGGVVFLGGMARPEVPEDAIDTQLAALRLAREGRDGGVFVTVADTAVRPAGAWLGGLHGLATTIGGERAAVSVECERGDRGPAAVAVLIADELTRGAGDRRVRLAADGTRTTGRPARPELSDWPGLVLGPDQRIGADSIVLAHDGGAALVELARETKCRLVLLGHTPLNEEPPGLTDAHDERAILRKFAAGTGGPDGLAGMRSRARAVLAVREIRATIEACEAAGARVRYLSVDTAEALVTALDRVRADWGPITALLHGPVQEPAEKTDTVAGAQLTDLAAVLAATARDPLETIVLFSARKDHALVAAALDRVAEAEQVRRPGCLVRSLTWNSARTEKVQPERARPGTVSVSAEAYPWIGDHRPQDVPILPPAAGLGWMAAAVRAREPEAVLTGFRVLRAVATAEPQTVSITTSGREVSLINGAGEPCFRARFGAPGPDREWRPTGDPARFPGSSIYAGRPLFHGPLLRAIRSLGEIGPAGVVGTVLGAKALGWADDDLPVDLPALDGAMQLSGVWAMDEHRPVLPMGMAECRIHRWGRLPGPALCVVADGRRDDHGARCDTALVGPDGELWMELLGIEFVALPADAPALSAPRPDPANWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDA8</accession>
    <name>U5WDA8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562339.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38755"/>
    <dbReference type="EnsemblBacteria" id="AGZ46005">
        <property type="protein sequence ID" value="AGZ46005"/>
        <property type="gene ID" value="AFR_38755"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38755"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7842"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_2_11"/>
    <dbReference type="OrthoDB" id="1377545at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="278" mass="29109" checksum="3050866E1B8EC81A" modified="2014-01-22" version="1">MTSQAIITAGSAGIGFETARGLLRSGYAVTIIARDEARGASAVRTLRDEIAGAEVRFLAADLSSLGTVRDLGARLAQEGPLHLLVNNAGGMFAHRWETADGLEASFVLNHLTPYLLTSALTGVLAAGAPSRVVQVTSSAMMAAVPDFADVEVPGEHYGLAVTGRAKLANLSWALDVADDLHERGITLVVADPGPAATPNAAAMEIRMLPPAMRSMWDVIQQGVQRPAAEAARPILHAATAPDLSPGTLVDAEQLRQHVTPEVTKAVRDLTERLLGPHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDU6</accession>
    <name>U5WDU6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_39705</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562528.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39705"/>
    <dbReference type="EnsemblBacteria" id="AGZ46195">
        <property type="protein sequence ID" value="AGZ46195"/>
        <property type="gene ID" value="AFR_39705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8033"/>
    <dbReference type="eggNOG" id="COG2148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024920_3_3_11"/>
    <dbReference type="OrthoDB" id="1759731at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017475">
        <property type="entry name" value="EPS_sugar_tfrase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03025">
        <property type="entry name" value="EPS_sugtrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="9">
        <location>
            <begin position="406"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ46195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="599" mass="66443" checksum="7E2B93FC816C11F0" modified="2014-01-22" version="1">MHQDLSEGHEATLGEPTVLLPTVQRRDVQPVDPHPENYGPANHPPANHGPRPHRPSPHRSRNTKIPAPARPPGDDTGEIVDNAPPLAGAGGHDPAERARNRAVERHWSPERNKQSAARPPTRPFIRVRGRHAAMSRRQRWERRYVRTLVLCDLLAGVAAGAVTFGLRFGDEVTTYNRSYLLLSALLPVVLLAVLVVSRAYERRFLFVGTDEYQRVFRGGVGLIAGVAVVSYALDLDLARSYVLAALPTAIGTAVVLRFALRKRLHMSRARGESLRRVLVVGHELSVIGITRQLRREKYHGLEVVGACLPPNHDGVIDLPVFGTFDNVANAVDLADADTVVVLSCPELDGAVLRRLAWRLERDEVDLVVASALIDVAGARTTIRPFDGLPMLHVDHPRLHGGARIVKDLVDRFGALALLILFGPLLLAVALCVRVTSRGPVLFRQVRVGRDGSEFRIFKFRSMYVDAEARLAELRHLNEYDGVLFKMRDDPRVTPVGRWLRRLSLDELPQLLNVLAGQMSLVGPRPPLPSEVAAYADDVRRRLAVKPGMTGLWQVSGRSDLPWEEAVRLDLRYVENWSLSLDLVILLRTMTAVVRSSGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE80</accession>
    <name>U5WE80_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562564.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39885"/>
    <dbReference type="EnsemblBacteria" id="AGZ46231">
        <property type="protein sequence ID" value="AGZ46231"/>
        <property type="gene ID" value="AFR_39885"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39885"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8070"/>
    <dbReference type="eggNOG" id="ENOG50347NI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1064091_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="260" mass="28419" checksum="146FA0362C07BFD5" modified="2014-01-22" version="1">MPTSVLLAVLAAAGLLALAPALVRRYDATERLAAERASSTARVLQRRRRLRSVPGRRPVNPGRQYLVTSRTLTGDPAPVSAAPVSAPPARRLRLVPARGRVRRPPVRKRQTPAVVRRRRVFAALLLLNLIELVGVLAVGPGFWISFAVTGTLLGIYLVHLRNRAIADRHRRRLEAREAAWLAARQAEVRREQARRAAARREAQRRLAAQKEAVRRAAMGLDRDPSDLPAAVNGGSVSYRRGGGLRGRAYEAGGRGSSHPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEM1</accession>
    <name>U5WEM1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative RNA polymerase ECF-subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_40710</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40710"/>
    <dbReference type="EnsemblBacteria" id="AGZ46396">
        <property type="protein sequence ID" value="AGZ46396"/>
        <property type="gene ID" value="AFR_40710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8239"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3194952_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="4">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="45" mass="4926" checksum="0D4583E48A57FB56" modified="2014-01-22" version="1">MTSDFDAFCTATVRRVVLYLYAVCGDLGDAQDLAQEAYARAISHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WEV2</accession>
    <name>U5WEV2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative secreted subtilisin-like serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_41110</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562808.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41110"/>
    <dbReference type="MEROPS" id="S08.091"/>
    <dbReference type="EnsemblBacteria" id="AGZ46476">
        <property type="protein sequence ID" value="AGZ46476"/>
        <property type="gene ID" value="AFR_41110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8321"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_17_3_11"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665820" evidence="6">
        <location>
            <begin position="30"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="7">
        <location>
            <begin position="151"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="546" mass="56377" checksum="B0AC465EFBDEBC01" modified="2014-01-22" version="1">MRRSFTAGAVATATGLAFLVGLPTSPANAATVSEYTVVADDGVSAADAVAAIEAAGGTVVSSNTAVGTYRVSSAAADFRLKAAKSGALIGAAARKPVGYAPKVSPAIEQPITEAARGSKPKGVAADPLDDKLWGLKMVKADQAHAIEKGEKGVTVGVLDTGLDASNPDLAPNFSNRLSRNFAPDLVDVDGPCEVEGCVDPVGTDDGGHGTHVAGTIAAAANGSGVTGVAPGVTLVELKGGQDSGYFFLDPVVNALTYAGDAGLDVVNMSFYVDPWLYNCKSNAADSPEAQAEQRAIIASMKRALNYAHARGVTLVGALGNQHDNLGDPNDDASSPDYGTPPYTRDIDNKDCLSLPAEGPHVIGVSAVGPSTSKSHYSNYGLEQISVAAPGGFTRDFFGTPQYNSAANNILSTYPLKVLQESGAVDANGEPTRATVFKDCTSGTCGYYTYLAGTSMASPHAAGVAALIISKYGHRDWWRGGLTMNPKLVELRLTTSATETACPEPRLRSYAQEGFDETFDAYCAGPKKFNSFYGHGIVNAYVAVGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF19</accession>
    <name>U5WF19_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoid-associated protein AFR_41805</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_41805</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YbaB/EbfC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41805"/>
    <dbReference type="EnsemblBacteria" id="AGZ46615">
        <property type="protein sequence ID" value="AGZ46615"/>
        <property type="gene ID" value="AFR_41805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8460"/>
    <dbReference type="eggNOG" id="COG0718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140930_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043590">
        <property type="term" value="C:bacterial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00274">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33449">
        <property type="entry name" value="PTHR33449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004555">
        <property type="entry name" value="UCP004555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00103">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="110" mass="11398" checksum="43BAE8DDEC926FFD" modified="2014-01-22" version="1">MRPGGQPNMQQIMKQAQKMQQQVAQAQAELAEAEVTGTAGGGLVTVVVTGLGEFKSVKIDPKAVDPEDVETLEDLVLAAIHNGSEAVRELTEQKMGPATGGLGGLSLPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFC5</accession>
    <name>U5WFC5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_41785</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41785"/>
    <dbReference type="EnsemblBacteria" id="AGZ46611">
        <property type="protein sequence ID" value="AGZ46611"/>
        <property type="gene ID" value="AFR_41785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8456"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="113"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ46611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="332" mass="35905" checksum="433AD5BA2BA8F3B2" modified="2014-01-22" version="1">MAYLIRRIFNAVLTLLVVTLVTFGIFFMVPKLTGSDPALLYIGKTADAVSLEGIRTKLGLADPIIVQYGKFLKGLAVGRDYSNGNDVTHCDAPCLGYSFKTDQEVTPLLLSDIPVTLSLAIGAAVLWVLIGVATGVLSALRRGTFLDRAAMTGALAGVSLPIYFTGLLASAIFIYWLGWLPNALYTPFLENPLLWAQGLILPWITLAFLFAATYARLTRANMLETLGEDYIRTARAKGLRERTVIGKHALRSGLTPLVTVFGLDFGALLGGAVLTEAVFNLRGLGFQALQGIRQNDLPIILGVTLIAAFFIVFINLIVDLLYGVIDPRVRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPQ3</accession>
    <name>U5VPQ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357727.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02475"/>
    <dbReference type="EnsemblBacteria" id="AGZ38784">
        <property type="protein sequence ID" value="AGZ38784"/>
        <property type="gene ID" value="AFR_02475"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02475"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.500"/>
    <dbReference type="eggNOG" id="ENOG5033V9Y">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100213_0_0_11"/>
    <dbReference type="OrthoDB" id="1841916at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="163" mass="17729" checksum="02FE1613F470B763" modified="2014-01-22" version="1">MTPVLVDDHFLLLDPEWQPDDSTATPPFEAVIGLWPRQADGSVGPFRSNPEYVPRYENSPSDPVDALLRLALRGDAELEDLQAVLRDTVVDLALNGDGRPLIQDSCVVVTTSALHRSRVSAPDWRQAGLTDVLAVLADWDLLINPGGPAPVRLAGAFVRETAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ68</accession>
    <name>U5VQ68_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-keto-myo-inositol dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03365"/>
    <dbReference type="EnsemblBacteria" id="AGZ38962">
        <property type="protein sequence ID" value="AGZ38962"/>
        <property type="gene ID" value="AFR_03365"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03365"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.677"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059523_0_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="69"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="28425" checksum="B29D55EC06119160" modified="2014-01-22" version="1">MPGWGYQLPADRVLREMREVGLTATEFGPDGFLPTDEILRDLGLSAIGGFVPVILHDPGHEPAVDFERFVAAGADTVVLAAATGQDGYDSRPVLDARGWQSLLTNLDKLAAAARERGLRAVLHPHVGTMVEQTAEVERVLDGCGIGLCLDTGHLLIGGTDPVVLAQRAGDRIAHVHAKDVDDSVAAEVRAGRLGYTEAVGAGMYKPIGDGDVDFAAVAAALQRHDYDGWWVLEQDTILTGEPAGEGPIADVRLSAGRLREILAAQAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQE8</accession>
    <name>U5VQE8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_03725</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357979.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03725"/>
    <dbReference type="EnsemblBacteria" id="AGZ39034">
        <property type="protein sequence ID" value="AGZ39034"/>
        <property type="gene ID" value="AFR_03725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.752"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_24_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665142" evidence="2">
        <location>
            <begin position="29"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="200" mass="21366" checksum="3374AC9E7A72E8EA" modified="2014-01-22" version="1">MKSERRTQDQRSATTRAALISAARPLFAASGFAAVSTEAIVRAAGVTRGAMYHQFADKTELFAAVFEAVEAELTQRIDAAVGSSGESDPITLMKLGAATWLDAGAEPEIQRIVLVEAPAVLGWERWREISMRYGMGLVKSLLEYAITAGRVPAQPVEPLAHVLIGALDEASMYVARATDPAQARREVGAVIDQLVQSLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQH1</accession>
    <name>U5VQH1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">rplB</name>
        <name evidence="7" type="ORF">AFR_04520</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358174.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04520"/>
    <dbReference type="EnsemblBacteria" id="AGZ39193">
        <property type="protein sequence ID" value="AGZ39193"/>
        <property type="gene ID" value="AFR_04520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.913"/>
    <dbReference type="eggNOG" id="COG0090">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_2_1_11"/>
    <dbReference type="OrthoDB" id="961486at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_B">
        <property type="entry name" value="Ribosomal_L2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_L2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L2_C" evidence="6">
        <location>
            <begin position="125"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="224"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="279" mass="30605" checksum="3F7030F48BD38896" modified="2014-01-22" version="1">MAIRKYKPTTPGRRGSSVADFAEITRSTPEKSLLAPLPKKGGRNVHGRITTRHQGGGHKRQYRLIDFRRNDKDGVPAKVAHIEYDPNRTSRIALLHYADGEKRYILAPKDLKQGDRVESGVGADIKPGNNLPLRNIPVGTTVHGVELRPGGGAKLARSAGVGIQLLGREGVYATLRMPSGEIRRVDIRCRATVGEIGNAEQSNINWGKAGRMRWKGKRPTVRGVAMNPVDHPHGGGEGKTSGGRHPVNPAGKPEGRTRRKGQESDRMIVRRRYATRKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQJ3</accession>
    <name>U5VQJ3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357992.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03790"/>
    <dbReference type="EnsemblBacteria" id="AGZ39047">
        <property type="protein sequence ID" value="AGZ39047"/>
        <property type="gene ID" value="AFR_03790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.765"/>
    <dbReference type="eggNOG" id="ENOG502Z802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144146_0_0_11"/>
    <dbReference type="OrthoDB" id="1565310at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="153" mass="16949" checksum="472C90970DEB0144" modified="2014-01-22" version="1">MPYVVDFATVSTVGLESSPVADALAGLRANEARYFKNKYDHVFTVEPASDAAEAIAWVSRILEQERGIVISSRPLEATAFQVENIRMAYVFYENGLSINVMYTVDDGGKRAVGFKLSDGMDVPEELGSFKFARQKSKLAGTIRGSYFVIKNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQX4</accession>
    <name>U5VQX4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 12" type="primary">rpsE</name>
        <name evidence="12" type="ORF">AFR_04590</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.</text>
    </comment>
    <comment type="function">
        <text evidence="7">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 9">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358199.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04590"/>
    <dbReference type="EnsemblBacteria" id="AGZ39207">
        <property type="protein sequence ID" value="AGZ39207"/>
        <property type="gene ID" value="AFR_04590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.927"/>
    <dbReference type="eggNOG" id="COG0098">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_1_2_11"/>
    <dbReference type="OrthoDB" id="1505038at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_B">
        <property type="entry name" value="Ribosomal_S5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005712">
        <property type="entry name" value="Ribosomal_S5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01021">
        <property type="entry name" value="rpsE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="11">
        <location>
            <begin position="38"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ39207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="20946" checksum="FE88BFD04D301E57" modified="2014-01-22" version="1">MPGQQRRGGGTGGNNEGGRRDNRREGGRGNAPVEKTPHLERVVAINRVAKVVKGGRRFSFTALVIVGDGDGTVGIGYGKAKEVPAAIAKGVEEAKKHFFKVPRIAATIPHPITGEAAAGVVLLKPASAGTGVIAGGPVRAVLECAGIHDVLSKSLGSSNPINIVHATVAALKGLESPEAVAARRGLPVEDVAPAAMLASRAEAAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR69</accession>
    <name>U5VR69_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="6">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">sucC</name>
        <name evidence="9" type="ORF">AFR_05090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358817.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05090"/>
    <dbReference type="EnsemblBacteria" id="AGZ39307">
        <property type="protein sequence ID" value="AGZ39307"/>
        <property type="gene ID" value="AFR_05090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1029"/>
    <dbReference type="eggNOG" id="COG0045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_4_0_11"/>
    <dbReference type="OrthoDB" id="316012at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_synthase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="PTHR11815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="9"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding; shared with subunit alpha" evidence="6">
        <location>
            <begin position="318"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="392" mass="41007" checksum="5AE656D523563360" modified="2014-01-22" version="1">MDLFEYQGRDLFERHGLPVLGGGVAETPQEARAIAERLGGRVVVKAQVKVGGRGKAGGVKLAADADEAEARATDILGMDIKDHTVHKVMLAETADIKEEYYFSYLLDRANRTFLCIASVAGGMEIETVAEEDPERVAKIAIDANKGVDEVTAREIVTAAKFPADVADQIVDIAVKLWQAFVAEDATLVEVNPLAKVGDGRVLLLDAKITLDENAGFRHPDHEALVDQSAVDPLEQAAKAKNLNYVKLDGEVGIIGNGAGLVMSTLDVVAYAGEGHGGVKPANFLDIGGGASAQVMANGLEIVLGDPAVKSVFVNVFGGITACDEVANGIIQALALLGERGEAVTKPLVVRLDGNNAEAGRAILDAANNPLVQRVDTMDGAAERAAELAAAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR79</accession>
    <name>U5VR79_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_05945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359009.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05945"/>
    <dbReference type="EnsemblBacteria" id="AGZ39478">
        <property type="protein sequence ID" value="AGZ39478"/>
        <property type="gene ID" value="AFR_05945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1208"/>
    <dbReference type="eggNOG" id="COG1793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204329_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="68" mass="7802" checksum="1F8E4F2A6E5FA0FA" modified="2014-01-22" version="1">MSTGEVEGAMQTPDGQWRVEIVRRRRTRWYRIVHGEDVLDWLSIAAVERILDEAGVDRRLLIEVGPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRM0</accession>
    <name>U5VRM0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoserine phosphatase rsbP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_06100</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359040.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06100"/>
    <dbReference type="EnsemblBacteria" id="AGZ39509">
        <property type="protein sequence ID" value="AGZ39509"/>
        <property type="gene ID" value="AFR_06100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1239"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_43_8_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="33"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="207"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="386"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="602" mass="64560" checksum="5F930E5788AF91D2" modified="2014-01-22" version="1">MAEDAEAVAGGKAGSLADPARLQSLQRVCVGAEPDAAFDRFASLVKRLLDVPVALVSLVDDERQFFPGQVGLAEPWSGKRETPLTHSFCQYVVVEGVPKVFPDARIYAQVRDNLAIPELGVVAYAGMPLTDADGRTLGSLCAIDTKPRAWTRAELETLNDLAEACSSELRLRIERELAEEARRHAENAHDQLAMLAELTETLATTIDIDEALSSLGSAVTPRLADWCLISLVDPDGSVRQVSAAHHDPARAEDVTRFAELMTTGLGAKSIVLAVQRTGMAIRRDGETPANILDRTSNPEMADLAARLGYASFVVAPITAPVNHRVLGSITLVNGPSRPPFGDADERTGTDIGRRAGLALDNSRLYRQQRHVAEVLQQSMLTELPTIPGVELHARYLPAQDGAAVGGDWYDAFAQPDGSVMLAVGDVSGHDIEAAATMGQLRNLVRGDAYGREDEPGPLLTQLDRAVRGLRVPASATAILARMSRDGDDYTVAFSNAGHPPPLLLLPDGTVEIWWVRPEPLLGLALRNERSTHHRRVPAGSTLVLYTDGLVETPGRMLDDGIATIEAALHGNAALPGEELCARLLDAAARRADDIALLLIRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSB7</accession>
    <name>U5VSB7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA ligase</fullName>
            <ecNumber evidence="5">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ligB</name>
        <name evidence="8" type="ORF">AFR_07325</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07325"/>
    <dbReference type="EnsemblBacteria" id="AGZ39754">
        <property type="protein sequence ID" value="AGZ39754"/>
        <property type="gene ID" value="AFR_07325"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07325"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1495"/>
    <dbReference type="eggNOG" id="COG1793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005138_6_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="SSF117018"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_LIGASE_A3" evidence="7">
        <location>
            <begin position="304"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="517" mass="54865" checksum="2652EC5F8F4D2706" modified="2014-01-22" version="1">MSYPAARMGFVQFIDLAATSAAVAATAGRKAKIDLLADALRRLERDEIAPGSAYLAGELRQRQTGVGYASLRDRPAPAAEPSLTVAEVDAKIAAISVVAGTGSQARRRELVVDLFGRATDAEQRLLVGLFSGELRQGAQAGLLADAIARAAEVPATAVRRALLLSGDLKQVAVAALSGGAVALSEFSLRVGTPLTPMLASSAPDVAAALLATGAPATVDVKLDGIRIQVHRSGDEVAVFTRSLDDITPRLPEVVAAVRALPGREFVLDGEAMALDARGRPRPFQETSSRAATQGGKAGPALVPYFFDLLHLDGTDLLDEPGRVRWAALADALPGSLIVGRRTVETEEEAAAAFASALAAGQEGVVIKAQEAPYDVGRRGAAWVKVKPRHTLDLVVLAVEWGHGRRKGWLSNLHLGARDPRTGGFVMLGKTFKGLTDELLRWQTERFKELAVDDSGWVVTVRPEQVVEIAFDGVQTSPRYPGGVALRFARVLRYRDDKRAEEADTIDMVKAIGSPSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSX9</accession>
    <name>U5VSX9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359589.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ40058">
        <property type="protein sequence ID" value="AGZ40058"/>
        <property type="gene ID" value="AFR_08845"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08845"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1800"/>
    <dbReference type="eggNOG" id="ENOG5033AWH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120330_0_0_11"/>
    <dbReference type="OrthoDB" id="2006751at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="195" mass="20984" checksum="37CA4EB852DBC8AE" modified="2014-01-22" version="1">MDDNEHLPDDDRRPHLALERHLTVGRHVYQLTASGTGEDRVNLTVVGWSPGGEVVSELSGGISPDDLTAVADALTSTLAGLGTLRRQRLKPAKPEPARRHPHQGARWSATDDERLVTRFRDGASPKVLMEEFGRSRGGINSRLVHLGLIAPEDAPTSRLPSERLLTVRGPSSSQSNLPAEADSEPDGKEGAIEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT46</accession>
    <name>U5VT46_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359712.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09220"/>
    <dbReference type="EnsemblBacteria" id="AGZ40133">
        <property type="protein sequence ID" value="AGZ40133"/>
        <property type="gene ID" value="AFR_09220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1877"/>
    <dbReference type="eggNOG" id="ENOG50330Y1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111152_1_3_11"/>
    <dbReference type="OrthoDB" id="2075110at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="148" mass="16054" checksum="C05933CB8A2A3128" modified="2014-01-22" version="1">MLNALEVLTTVIVGVMVGVEFAVAFVINPILCALPRDSGLLGRAHGGRMLGAVMPVWYITSLVLVAIWAVAGWGDEGTGLVVTAGAFLLVSVLMSVVLLVPINNRTSTWTPDNRPDDWKQQMKRWDRLHYIRVVVIIAAFTMLVAALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VT74</accession>
    <name>U5VT74_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative fluoride ion transporter CrcB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">crcB</name>
        <name evidence="10" type="ORF">AFR_02835</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the CrcB (TC 9.B.71) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357799.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02835"/>
    <dbReference type="EnsemblBacteria" id="AGZ38856">
        <property type="protein sequence ID" value="AGZ38856"/>
        <property type="gene ID" value="AFR_02835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.569"/>
    <dbReference type="eggNOG" id="COG0239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114342_2_1_11"/>
    <dbReference type="OrthoDB" id="1942863at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="CrcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="CrcB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="PTHR28259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ38856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="118" mass="12019" checksum="B2A0964ABFD59CA9" modified="2014-01-22" version="1">MTLLLVALGAAVGAPLRYLTDRAVQARHSSGFPWGTFTVNIAGSLLLGFLLGLPVAPWLAALLGTGFCGALTTYSTFSLEILRLARGGERFLAAAYAIGSVLAGLGAAMVGVLIAQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTU5</accession>
    <name>U5VTU5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_04180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358070.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04180"/>
    <dbReference type="EnsemblBacteria" id="AGZ39125">
        <property type="protein sequence ID" value="AGZ39125"/>
        <property type="gene ID" value="AFR_04180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.844"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049717_1_0_11"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="32998" checksum="408FFB2DE628C4FA" modified="2014-01-22" version="1">MSHHVIVGAGPIGSAVAHQLVDQGKSVRMVTRSGSGPEHPLIERVAADATDAAKLTELTRGAEAIYNCANPKYTQWEALWPPLSGAMLTAARETGAVLATTGNLYVYGPVPGGRMTERSPLAATGRKGRVRIKMWQEALASGVRTVEVRGADYVGAGAASIFSAVLLPAIAKGRTAWAPADIDAPHTFTYTGDMARALVQLAADERAHGRAWHVPSPAPISIRELAQRYYAVSGAKPVPLRPLPRFVMRTAGLAIPMAREMAEMDYQFYAPFIMDSTETEQTFGLTPTDLDTALREVAADAEATASRTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU01</accession>
    <name>U5VU01_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative cation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_04035</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358041.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04035"/>
    <dbReference type="EnsemblBacteria" id="AGZ39096">
        <property type="protein sequence ID" value="AGZ39096"/>
        <property type="gene ID" value="AFR_04035"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04035"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.815"/>
    <dbReference type="eggNOG" id="COG0168">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026429_0_0_11"/>
    <dbReference type="OrthoDB" id="756220at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="446" mass="47932" checksum="CB6F13518EDA1191" modified="2014-01-22" version="1">MRSRSPWRYPARIVPVAFLGAILIGTLLMMLPAARAEPGNAPFVTALFTATSAVCVTGLAVVDTPTYWSGFGQVLLTVLSQIGGFGIMTLATLLSLLVSRQLGLRSRLMAQAESSGLLGGNVGGVLIRVAIVMFASETAISIVLTLRFWLHYDYPFGRAVWEAVFHAVQAFNNAGFALYPDSLVRFVGDWWICVPLSLGVLAGSIGFPVLFELAREWRKPGYWSTHTRLTVWGTLLLSLTGFLVFLTFEWTNPNTLGPLGTPAKVLAAFTQDVMTRSGGFNSINLGGMNTETIAVTNGMMFIGGGSASTAGGIKVTTFLLLAFVIWAELRGEPDVVIRKRRIAEETQRQAMTVALLGVALVASGTLALIGLTDHVPFDRALFEVTSAFATVGLSTGITPTLPQSAQVVLVILMYVGRVGTIAVGTAIALNTRRRLYRYPEERPLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU08</accession>
    <name>U5VU08_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">X-Pro aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_10970</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360400.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10970"/>
    <dbReference type="MEROPS" id="M24.033"/>
    <dbReference type="EnsemblBacteria" id="AGZ40483">
        <property type="protein sequence ID" value="AGZ40483"/>
        <property type="gene ID" value="AFR_10970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2234"/>
    <dbReference type="eggNOG" id="COG0006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_1_0_11"/>
    <dbReference type="OrthoDB" id="415910at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP_N" evidence="4">
        <location>
            <begin position="43"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ40483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="488" mass="54095" checksum="09DE89F389048CB5" modified="2014-01-22" version="1">MAEQNEGTTPKTESHDPDFPEAFLQFMRSGWRDDTLNVTPRPETPNYAKRRAELSDAFPGETLIIPTGTEKVRANDTDYPFRPGSDFVYLTGDTDPDSVLIMRPSGSGHDAVVYSRQRSSKETDEFFRSRDGELWVGRRHTLREKSTELGLETAPWVGLGQALAECAPARTRVLRGLDPNVDRAILAYEPDPRVRRDRALAGVISELKLVKDEWEIAQLQDAIDATVRGFEDVARVLPADRGVKERLLEGVFGMRARVDGNDVGYGSIVGAGAHATILHWVRNTGVTKPGELLLMDMGVESHTFYTADVTRTIPVSGTFTPLQRQVYDIVHASQQAGMDAVRPGVKFQDVHLTCMRVLAEGLNDLGLLPVSVDEAMSEESTVYRRWTLHGFGHMLGIDVHDCSAARKEKYRDGELGEGYVLTVEPGLYFQPEDELVPEELRGIGIRIEDDVLVTADGSRNLSAGLPRTADQVEAWLASQREAGPRLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU15</accession>
    <name>U5VU15_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_04110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358056.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04110"/>
    <dbReference type="EnsemblBacteria" id="AGZ39111">
        <property type="protein sequence ID" value="AGZ39111"/>
        <property type="gene ID" value="AFR_04110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.830"/>
    <dbReference type="eggNOG" id="ENOG5032YJA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168842_1_0_11"/>
    <dbReference type="OrthoDB" id="1975208at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021456">
        <property type="entry name" value="DUF3107"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11305">
        <property type="entry name" value="DUF3107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="74" mass="8196" checksum="7E8408381DE2F63C" modified="2014-01-22" version="1">MEVKIGVQYAQRELVLESAQTQAEVEQAVTEAMAKDGVLTLTDEKGRKVIVPIAKVAYVEIAEKSERPFGFTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUI5</accession>
    <name>U5VUI5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">T-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_05060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358806.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05060"/>
    <dbReference type="EnsemblBacteria" id="AGZ39301">
        <property type="protein sequence ID" value="AGZ39301"/>
        <property type="gene ID" value="AFR_05060"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05060"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1022"/>
    <dbReference type="eggNOG" id="COG1605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139648_2_0_11"/>
    <dbReference type="OrthoDB" id="2035765at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010958">
        <property type="entry name" value="Chorismate_mutase_highGC-bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="SSF48600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01808">
        <property type="entry name" value="CM_M_hiGC-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="96" mass="10554" checksum="BCEF102B235A78BA" modified="2014-01-22" version="1">MSADVKAQPTGTDAAPAAEEITAIRERIDEIDQSLIDLWLERSRLSQQVGRTRMASGGTRLVLSREREIVERFREALGPDGTQVALLLLRAGRGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUS2</accession>
    <name>U5VUS2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358932.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05560"/>
    <dbReference type="EnsemblBacteria" id="AGZ39401">
        <property type="protein sequence ID" value="AGZ39401"/>
        <property type="gene ID" value="AFR_05560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1128"/>
    <dbReference type="eggNOG" id="COG3087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_242700_0_0_11"/>
    <dbReference type="OrthoDB" id="338654at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="582"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="608"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="632"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="654"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="678"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="705"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="779"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="805"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="836"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="863"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="894"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="937"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="965"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="989"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1013"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1038"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1093"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1120"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1148"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1174"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1235"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1291"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1316"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1341"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1377"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1401"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1425"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1452"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1483"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1508"/>
            <end position="1524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1536"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1589"/>
            <end position="1608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1614"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1661"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1696" mass="171937" checksum="3317AEC2F6430645" modified="2014-01-22" version="1">MAQSQEPDGYDDELARIDASIADLKIRDMAAAKERTQIASKIQAAQFQRDILAHANQQKKRPAKTTRRVRRRPEESTGAPSWSEPGTARTPPPGAATDGVTMLLDDPPPTERPAPPPRPPRRPRVAPLPPEPEAHAPHEPETSSQSVQNILLGLSALVLGVAAIVFAGAASNAVGRAFILAIATAVALGVAPMVSRRGLTSTAETLAAVGLVLLPMTMFALHGSRALGGTAVPSTVFLGITLLISAVASFLYAGVTRLAAPRYATVVAVQPVPPLLAYPAIESPAGWALALTAVALIDLLLLTTVIRKGRLLPRWPLGRPVAGDRETLAEADARQRGADDFAAREDDPADPASYEDAPARPESLPEEPDLIIDGLTGRRRWKWLPTRIFPGPRPAGAPAAGTVPLAPPATPPSAGWLRGLTFGLLCLAAAGAVVYASAALLGADAVIDAVRSGLILVLAALTVSAAARMVDHILARNIAGGVLALAVIAAVARIAAVASPAWTLAAAAAAVALTGAVVRMVPDEVRRGPQYASAGALTLIGLFVAVDALRAAIAPVQAARPIWNADTAAYATRIAEAAGQSGWLLALSALLLTIAAALALPTEYRHEGAVTGVALTALAVPASLGLPWSEAPWPLVLAAIGLGGAGLMAPTRRIAIAHIAAAGVVGLFGAAAALSASWLTAAVLLALAGAGVMVAVAARQIPVRLYAWLIGDWASGAAALAIPGAVVTAVLATSDPGGGPPPTEAVTVPALALGFLAVAGTLTYAAVFQVARREISLPMTGGAGAGAVAMALAALLAPGATAPDIWVGALLLAAAGLLFFAKSLDAGRRADRMVDGPDIAAAAATVAVCGALARVAALAFPEAPLAVAAIVVLIVAFGVRALPEDWRRGPVRGVGAAGIVIVAIAGWQALAGGLRIISAPGPIWASEIGAYSTTPPAGAWQAPVALVIAAVAAAVALPRPAKYDVSALCVALATVGAPAAFGLPWWSPLLIGGAVALAYGMGAVAATDPRAAIARAAVAAVVALHAAGAGLVRPWSTAIALGLITLTGILVAWMARADLTPGSHRQWAETADGDEVVNLALDDTGMPRHRAQIGGAATAAALFAAPGVLAAMAADQGNSAQVVLTAALAGSSVALALLALAARWVPQYLPWATFGLVFGAFVTAIASIPSDYPTALYAAAAALLGVVAELLRGATPAPGLTVAPNRFWGEGGYVRPRWTAIRPSGLRGRWLVDPATGAVVVAALPTALALISIAPALKASLLDPMQQLSAIWEGPVAAVATPASGSVDGTSVLAAVLLTVAAALAALGFGGKPAEAVPVILPGLAITLLIAPIALNAQWPAATSTALIVFTISMLGLALTPPPIATRATLLRTTRNIVFVIGLLAGGAGLAGSLATQQLTLFTLGAAIGVGLVGAIAGRSRHARILGWIFTAVMGQFFVLTVALVAGLTPPWAAFGVLAVGAVLLVLEAAIPRLGLPEYRQEATTVEWSGYASALLAGALAYSSPAHLAALLAAWGAVLGLAATRPGRTPNQRRNLFWLAVGFEIIGIWLFIAIADVALPEAYTLPFAALALLVGILESRQRPDLSSWAAYGPALVAAFVPTIGIVVATDAGDLRELLLLLSAVATLIIGSRMQQQAPVVVGAVATAIATIHFATTLVGPWLVLVPVGVILLFLGATNENRRRTQERLRGALVRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV28</accession>
    <name>U5VV28_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_11790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360692.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11790"/>
    <dbReference type="EnsemblBacteria" id="AGZ40647">
        <property type="protein sequence ID" value="AGZ40647"/>
        <property type="gene ID" value="AFR_11790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2401"/>
    <dbReference type="eggNOG" id="ENOG5032R9R">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174618_0_0_11"/>
    <dbReference type="OrthoDB" id="1812905at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="100" mass="11606" checksum="96B6F27D27914E29" modified="2014-01-22" version="1">MTHLPSDVRRSLRMFAFFVGNGTVDVELLGDIDYRADLLEFGSDLERVFTIFANVLEVDEFGTVTNHDHAQRRAAQWIRSSVDPDYEPEPPFEDWEFELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV34</accession>
    <name>U5VV34_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RecQ family ATP-dependent DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_11840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360712.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11840"/>
    <dbReference type="EnsemblBacteria" id="AGZ40657">
        <property type="protein sequence ID" value="AGZ40657"/>
        <property type="gene ID" value="AFR_11840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2411"/>
    <dbReference type="eggNOG" id="COG0514">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001103_9_7_11"/>
    <dbReference type="OrthoDB" id="54580at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="24"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="221"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ40657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="539" mass="57884" checksum="82B8A3C94F88CA12" modified="2014-01-22" version="1">MADVTALAASVFGFALRPSQRSAAEAVTGGRDTLAVLPTGSGKSAIYQVAGLAREGVTVVVSPLIALQRDQVRSLSGLGLRAVLLNSSLHAAQRAEVLATIRAGETDFVMLGPEQLANDETLRELTGTPRPITLVAVDEAHLVSEWGHDFRPEYLRLADVIDAFGRPPVLALTATAAPPVQADITRQLRMREPQIIVADFDRPGITLAVRRTRHGLPEDQAIDDRTVEVVLAHQTPALVYALTHARCESLAERLRLDSYRVAAYHAGMPAAERGRVQDGFFAGTIEIVVATSAFGMGIDKPDVRTVVHAGVPGSLDEYYQEIGRAGRDGEPANAILVYDPRTVRIPRLLAARSRIAAGTIHAVVDVLESRSGRVTYQELAEASFVSKHMVERVVAELLELDALEADGHDAVLIPKRLPRTTFASVAEAGDRHQAVLTSRIDAARHYAETTRCRRAELLGYFGEHYEAPCGTCDNDNTAAAAPSGMSAPELPGGVGVHHRLWGPGTLLSRDEHELTAVFASVGYRHLTPAVLANGVLTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV92</accession>
    <name>U5VV92_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360987.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13210"/>
    <dbReference type="EnsemblBacteria" id="AGZ40928">
        <property type="protein sequence ID" value="AGZ40928"/>
        <property type="gene ID" value="AFR_13210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2682"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_2_2_11"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13463">
        <property type="entry name" value="HTH_27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="18128" checksum="343DD52B96559C58" modified="2014-01-22" version="1">MTSSPRWLDDEQQKLWQDLRTVVVGLPMLLDRQLQRDEGISNFEYSVMARLSMVEKRTMRLSDLATQCDSTLPRLSKLMVRFERQGWVTRCPDPDNGRYTLAILTDAGLRKVEDSAPAHVEQVRQLVFDPLGAAQQRQLSTSLERIAELIRERLDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVR0</accession>
    <name>U5VVR0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_07210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359262.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07210"/>
    <dbReference type="EnsemblBacteria" id="AGZ39731">
        <property type="protein sequence ID" value="AGZ39731"/>
        <property type="gene ID" value="AFR_07210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1471"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="218" mass="23985" checksum="234BF4C7D81E2B56" modified="2014-01-22" version="1">MRILVAEDEPLLADAVAQGLRHDAHAVDVVYDGGAALERAGVNDYDVLILDRDLPVVHGDEVCRRLAGRDATMRILMLTAAAEIDDRVEGLALGADDYLPKPFAFRELSARVAALGRRSRPAVPPVLRRAGISLDPHRREVFRDGRYVPLSRKEFAVLAELLRADGTAVSAEQLLEKAWDENADPFTHAVRMTILKLRRKLGNPPVLLTEPGVGYRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VVZ3</accession>
    <name>U5VVZ3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359352.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07660"/>
    <dbReference type="EnsemblBacteria" id="AGZ39821">
        <property type="protein sequence ID" value="AGZ39821"/>
        <property type="gene ID" value="AFR_07660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1562"/>
    <dbReference type="eggNOG" id="COG1032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011543_3_2_11"/>
    <dbReference type="OrthoDB" id="970297at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023862">
        <property type="entry name" value="CHP03960_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045784">
        <property type="entry name" value="Radical_SAM_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19864">
        <property type="entry name" value="Radical_SAM_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03960">
        <property type="entry name" value="rSAM_fuse_unch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="260"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="650" mass="72948" checksum="DD492271E1E7422A" modified="2014-01-22" version="1">MSVKSVFPQLEQLLPRVSKPIQYVGGELGAVVKDWDATTVRWALMYPDAYEVGLPNQGVQILYEVLNEQSDVLAERTYAVWPDLEALMKENGVPQFTVDAHRPVKAFDVLGISFATELGYTNMLSALDLAQIPLNSADRDESHPVVLAGGHASFNPEPIADFIDAAVLGDGEEAVLEITGIIRQWKAEGCPGGRDEMLLRLARTESIYVPRFYDVDYLPDGRIQRVVPNRPDVPFRVHKRTTMDLDAWPYPKKPLVPLAETVHERYAVEIFRGCTRGCRFCQAGMITRPVRERSITTVGQMVKEGLEFSGFSEVGLLSLSSADHSEIGDMCSGLAEQYADTNVSLSLPSTRVDAFNIDLAQELSRNGRRTGLTFAPEGGSERIRKVINKMVTEQDLINTVVTAYSNGWRSVKLYFMCGLPTETDDDVLQIGRMAHEVIKAGRIATGTKDVKCTVSIGAFVPKPHTPFQWAAMERPEVIDNRLRMLKQEINSDRSLGRAIGYRYHDGEPSLIEGLLSRGDRRVGAVIRRAWDKGQRFDGWSEHFSYSRWVESAAEILPAFGVDLDWFTTREREQLEVLPWDHLDSGLDKDWLWQDWQDSTTEFEQDDCRWTPCFDCGVCPSQDTEIQIGPTGKKLLPLTPTTGLRVPAHNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW20</accession>
    <name>U5VW20_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_13580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361061.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41002">
        <property type="protein sequence ID" value="AGZ41002"/>
        <property type="gene ID" value="AFR_13580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2757"/>
    <dbReference type="HOGENOM" id="CLU_2314083_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="99" mass="10372" checksum="96500860A0181158" modified="2014-01-22" version="1">MLSIHHPRQTILPRTGESDARRPTTIRAALIGAGLGSWLGILTGVVLALSVAGHVWPAVSSGGLLIGAFVGALVGCVTHWAADRRGAEPRERSSCWRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW47</accession>
    <name>U5VW47_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chromate transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_14630</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361267.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41208">
        <property type="protein sequence ID" value="AGZ41208"/>
        <property type="gene ID" value="AFR_14630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2971"/>
    <dbReference type="eggNOG" id="COG2059">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018106_0_0_11"/>
    <dbReference type="OrthoDB" id="1884249at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014047">
        <property type="entry name" value="Chr_Tranpt_l_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004810">
        <property type="entry name" value="ChrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00937">
        <property type="entry name" value="2A51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ41208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="453" mass="47623" checksum="B7D110334DBD9BA5" modified="2014-01-22" version="1">MTGTLHDDVVPLRRAVKVWFLISLQTFGGPAGQIAVMQRHLVDDRRWIGQKRFLHALNYCMLLPGPEAQQLAIYIGWLLNGLRGGLIAGTLFVIPGVVALLGLSIVYVAFGDTTVVTGIFAGLAPAVVAIVAQAVWRVGNRALTDRVLLGPAVAAFVALAAFAVPFPVVILVAGGIGWALRRRAARTGDGPAAPDDGPPPLISDDALHQDRPSKRRASTILGAGLVVWFLPIAAVAALTGGNSVYTQQGLFFSGTAVVTFGGAYAVLAFVAQRAVEHYGWLSAGDMVRGLALAESTPGPLIMVVQFVAFLGAYHDPGPLNPWTAGVVASLLTTWVTFVPCFLFILLGAPYVERLRGNQSLSAALTGITAAVVGVIAQLGLYFAVHALFERTRTVTGGPLHLQLPDFASVRPIPVVIAVIAAALIFRWKWPVLRVLGVSALLGLIAGLTGLPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VW90</accession>
    <name>U5VW90_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_14905</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361322.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14905"/>
    <dbReference type="EnsemblBacteria" id="AGZ41263">
        <property type="protein sequence ID" value="AGZ41263"/>
        <property type="gene ID" value="AFR_14905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3027"/>
    <dbReference type="eggNOG" id="COG3507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016508_1_1_11"/>
    <dbReference type="OrthoDB" id="1622507at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001919">
        <property type="entry name" value="CBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012291">
        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041542">
        <property type="entry name" value="GH43_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00553">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17851">
        <property type="entry name" value="GH43_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00637">
        <property type="entry name" value="CBD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51173">
        <property type="entry name" value="CBM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665811" evidence="4">
        <location>
            <begin position="32"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="CBM2" evidence="5">
        <location>
            <begin position="28"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="642" mass="68773" checksum="0B11234BB95038B4" modified="2014-01-22" version="1">MRRTRPRLWIAAGLALAVSTAGLALATSARAAAGCKVTYTVTNQWQGGFGANVTIDNLGDPITGWRLGWSFGAGQTVAQLWGGTVSQTGAQVTVTNADYNASLPTGGSASFGFNGTSGASNPAPASFTLNGVACTGGTAPTTPAGGTFTNPVVWQDFADGDIIRVGDVYYYSASTMHYSPGAPVLRSYDLVNWEYAGHSVPRLDFDSNAYDLNGGRAYVKGIWASAFNYRPSNSTYYWLGCTEFNRTYVYTSANAGSGWGKKARINKCYYDAGLMFDNDTPYVAYGNGTISVAQLSSDLTSEVRSQAVYQTPSSIGTLEGARMYKRGNYYYIWLTRPANGQYVLRSTSPWGPYEQKQVLLNLPGPISGGGVPHQGGLVQTQNGDWWYMAFTDAYPGGRMPTLAPITWSADGWPVLQTVNGRWGATYPRPNLPWHPVTPMTGTDTFTGTTLGPQYEWNHNPDTSKVSVNNGLRLSTATVTGDLYNARNTLTRRIQGPSSTATIELDYSAMANGDRAGLAMLRDTSAWIGIRKDNGATRISMTNGLTMSTSGWTTTGTGTERAGAAISGGKVWLRVNADIRPGSGRTATFSYSTDGVTFIGLGPALTLNNDWQFFMGYRFGVFNYATQSLGGAVSVSRFTVSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWC1</accession>
    <name>U5VWC1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="8">2.2.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">1-deoxyxylulose-5-phosphate synthase</fullName>
            <shortName evidence="8">DXP synthase</shortName>
            <shortName evidence="8">DXPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dxs</name>
        <name evidence="10" type="ORF">AFR_14335</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D-xylulose 5-phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:12605"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361208.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14335"/>
    <dbReference type="EnsemblBacteria" id="AGZ41149">
        <property type="protein sequence ID" value="AGZ41149"/>
        <property type="gene ID" value="AFR_14335"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14335"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2909"/>
    <dbReference type="eggNOG" id="COG1154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_4_11"/>
    <dbReference type="OrthoDB" id="566385at2"/>
    <dbReference type="UniPathway" id="UPA00064">
        <property type="reaction ID" value="UER00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052865">
        <property type="term" value="P:1-deoxy-D-xylulose 5-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="PTHR43322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00204">
        <property type="entry name" value="dxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transket_pyr" evidence="9">
        <location>
            <begin position="320"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine pyrophosphate binding" evidence="8">
        <location>
            <begin position="151"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ41149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="641" mass="67979" checksum="3B62486CE0C9235E" modified="2014-01-22" version="1">MSERGQDLLGTIRGPQDLKGLDAEQMTALGAEIRAFLIAKVARTGGHIGPNLGVVELTLAMHRVFDSPRDRFIFDTGHQAYVHKMVTGRQEGFDRLKRRGGLTGYPSRAESEHDLVENCHASTALSYADGMAKAYALRGEARHVVAVVGDGALTGGMCWEALNNIAATRNPLVIIVNDNGRSYAPTVGGLADHLASLRLSPGYEKGLELIKDTLGKTPLVGKPLFGALHAAKRGIKDAVAPQAMFEDLGIKYFGPVDGHDLAAMESALARAKGYGGPVIVHAVTQKGRGYAPAEQDEADRLHAPGAFDPETGRQLAAPSLKWTKVFADELVALAGERPDIVGITAAMPESTGIDALAAAYPDRAFDVGIAEQHATTSAAGLALAGLHPVVAIYATFLNRAFDQVLLDVALHRLPVTFVLDRAGVTGPDGPSHYGIWDMSLLGVVPGLRVAAPRDAETLRTSLREAVAISDGPTVVRFPSGSVPATVPALRRVGSVDVLAETPERKDVLLVTVGRFAQLGVQVAAELEKRGIGVTVVNPLWVRPVPVELVGLAGAHRLVVTVEDGVRNGGIGDAVAKLLRDHELDVPLRDLGVPVQWIDHGTRAEILAEIGLTAADITERVQRWTEGLSTSDQPVRSSIGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWI0</accession>
    <name>U5VWI0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type 11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_08805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_08805"/>
    <dbReference type="EnsemblBacteria" id="AGZ40050">
        <property type="protein sequence ID" value="AGZ40050"/>
        <property type="gene ID" value="AFR_08805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_08805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1792"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099730_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="20"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="211" mass="22025" checksum="84B6270EEECEC41F" modified="2014-01-22" version="1">MSAWLAAAAPFLPGPGGRILDLGAGTGRFTAALGEVATVIACEPSAAMRAVFPGGQLLVGGSAEAIPLAAASFEAVWASQMVHHVKDLEAFAGNVRRVLKPGGHLLLRGGFGELPLTRWFPEAFAADAEGLLGVVTARLAEVGLTLTARIEVGQRYADSAQELVDKVRTRSLSNLAVLADDHFADGLRALEQDAPGLTYPLDEQLELVVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWN2</accession>
    <name>U5VWN2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transporters inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_14600</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14600"/>
    <dbReference type="EnsemblBacteria" id="AGZ41202">
        <property type="protein sequence ID" value="AGZ41202"/>
        <property type="gene ID" value="AFR_14600"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14600"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2965"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="112"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="317" mass="33835" checksum="0C704C14BA97D456" modified="2014-01-22" version="1">MTGGISTLADHKRRRIEELTARTSEAGGVSLIRDAGRRLLRNPTAIVGGAIILLFLVIAIFAPLVAPHDPVQRFPELTRDLTVDSIPGATAGHPLGSDPLGRDFASRMIYGARQTLFVGVLATLIGLLLGVLLGAIAGAVGGWVDVLIMRVTDVMLALPSLLLAITLVALASRSTQWTVIIAVAVVNVPIFARLLRGSMLAQRESDHVLAARALGVKRRDIVLRHMLPNSLTAVIVQATLTFAVAILDAAALSFLGLGDPDINRAEWGLMLGVDGVRYFEVRPELAYFPALAIIAVALGFTLLGESMREALDPKNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXC8</accession>
    <name>U5VXC8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_16675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361700.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16675"/>
    <dbReference type="EnsemblBacteria" id="AGZ41613">
        <property type="protein sequence ID" value="AGZ41613"/>
        <property type="gene ID" value="AFR_16675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3381"/>
    <dbReference type="HOGENOM" id="CLU_1243155_0_0_11"/>
    <dbReference type="OrthoDB" id="2179583at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="216" mass="22663" checksum="693913F71AF74D34" modified="2014-01-22" version="1">MYPAPPPMERPTTLRVSSALLFGVAALALVGAVIELLFVNTGLSVYRDTWTGETGSGFGSIFGATLDIFFAAGLSILAILNGHGRRNARVVTLVLGGLFFFCGGFATLADRPDGASRTTGDGTLGDILPTAYGITVAGLDVLITLGILGALVLLVLPPSNRFFINAQLIRDMPPPPVVVYLPQPVVPAPRGPEPPPHTGSIPAIDPWASQQDRHQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXK6</accession>
    <name>U5VXK6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_16225</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361602.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16225"/>
    <dbReference type="EnsemblBacteria" id="AGZ41527">
        <property type="protein sequence ID" value="AGZ41527"/>
        <property type="gene ID" value="AFR_16225"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16225"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3292"/>
    <dbReference type="eggNOG" id="COG4214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028880_2_1_11"/>
    <dbReference type="OrthoDB" id="1254678at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ41527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="418" mass="42992" checksum="1D438AC5F5912426" modified="2014-01-22" version="1">MTTQTTTTAGGVTVVKPTLAGHLHDYWGRVRGGDLGSLPAVLGLVVLSLIFGVARETFFSPLNFANLFTQGAQVIFIAMGLIFVLLLGEIDLSAGFASGVCGAVMAILLTNHGVAWYVAIPVALLTGVVIGLALGVLIAKVGIPSFVVTLAAFLGFQGLLLVLLGGGINISIRDEFVISLNNNNIPVLWSWILAIGSVAGYAFLQFSRIRARAARGLVTDPLGVVALRIGGLAVLVLATTAVLTQERAVNPAINSLKGVPIVVPIIAAFLIFWTFVLGRTTYGRHVYAVGGNQEAARRAGIPVDRIRISVFAIGSFMAAIGGIMAVSRASSVDPNTGGSNILLYSVGAAVIGGTSLFGGKGKVINAVIGGAVIAVIDNGMGLMGFSSGQKFIFTGLILLVAASVDALARRRAAGAGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXL0</accession>
    <name>U5VXL0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">AFR_10905</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10905"/>
    <dbReference type="EnsemblBacteria" id="AGZ40470">
        <property type="protein sequence ID" value="AGZ40470"/>
        <property type="gene ID" value="AFR_10905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2221"/>
    <dbReference type="eggNOG" id="COG1718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060067_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO" evidence="11">
        <location>
            <begin position="34"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AGZ40470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="294" mass="33355" checksum="7A47FE3D22E0F6FE" modified="2014-01-22" version="1">MRRGRRKFDDDEADFVKRGRIEPALSEDPDLPETGDRWSTWDNALHGPDPLPEWVRTEHGAVDTELGMLKTGKEADVYLVRRAVPDTDKVSLLAAKRYRDGDHRLFHRDAGYLEGRRVRRSREMRAMTNRTAFGKELIAGQWAAAEFDALSRLWRVGQEGGTISVPYPVQLIGTELMLEFIGDHKTGEAAPRLAAVRTDREGLEDLWRQMTEALSVLARTQIAHGDLSPYNTLVHEGRLVIIDMPQIVDLIANPQGAEFMARDVRNVANWFTARGLRVDQDELIERLSYEAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY27</accession>
    <name>U5VY27_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Succinyl-CoA:(R)-citramalate CoA-transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_17580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362061.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17580"/>
    <dbReference type="EnsemblBacteria" id="AGZ41794">
        <property type="protein sequence ID" value="AGZ41794"/>
        <property type="gene ID" value="AFR_17580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3563"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_2_0_11"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="42405" checksum="3AE4F3E4398F0CBD" modified="2014-01-22" version="1">MTLPLDDLRVVEFGQLLAGPFCGQLLGDFGAEVIKVEDPATGDPMRQWGREKPHGKSLWWPVVARNKKSITCNLRDPEGQALIRRLIDTADIVVENFRPGTLERWGMAPETLWKTNPGLIITRVTGYGQTGPYASRAGFGSIGEAMGGIRYVMGEPGSPPVRAGISLGDSLAAVYACLGTLVAVHSRGRTGRGQIVDSAIYEAVLAMMESLLPEWAVAGYQRERTGATLPNVSPSNVYPTGDGDLILIAANQDTVFGRLAVAMGEPELAADPRYATHSARGAAMAELDDHIARWTSGRAAEDLLGLLHANGVPAGRIFRAKDMFGDPHFAAREAIVTVPHPDFGELPMQNATPRLSETPGAVRTAGPALGEHNTEIYGGLIGLDETEQARLRAAGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY76</accession>
    <name>U5VY76_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362161.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41844">
        <property type="protein sequence ID" value="AGZ41844"/>
        <property type="gene ID" value="AFR_17830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3616"/>
    <dbReference type="eggNOG" id="COG4312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066898_1_0_11"/>
    <dbReference type="OrthoDB" id="1567592at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010296">
        <property type="entry name" value="DUF899_thioredox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05988">
        <property type="entry name" value="DUF899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="257" mass="29186" checksum="29F5D8F9D9883831" modified="2014-01-22" version="1">MTPDVVTREEWLAARKELLTREKELTRARDRLNADRRRLPMVRIDKPYRFEGPDGAVGLPELFEGRRQLVLHHFMFAPGWEAACASCSSAADSIAGLRQLHARNTTLVAVSRAPYAQIAAFRERMGWTFPWYSSYGSDFNYDFHATLDDRVTPVLLHFRTEDELAETGTPWTGGPWTESMRGGELPGLSAFLRVDDEVFHTYSTYGRGIEEFHNGYPYLDLTALGRQEAWEEPQGRATPLGLQVGSPGMRLPDEYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYA0</accession>
    <name>U5VYA0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362211.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17955"/>
    <dbReference type="EnsemblBacteria" id="AGZ41869">
        <property type="protein sequence ID" value="AGZ41869"/>
        <property type="gene ID" value="AFR_17955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3642"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015079_0_0_11"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="194"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="685" mass="75259" checksum="81BFC5708C7FAA68" modified="2014-01-22" version="1">MRISFLVHNVYGVGGTIRTVINLAEELSRRHQVEIVSILRRLDSPMLEISPRISVVALADLRPGSADSQDPRAREMSALVPVEEEFFRFYSRLTDERITTYLRRTSADVVVGTRSALNLCVARLGRTDQVRIAQEHMTQDSIPESVRNEMRRHYPRLSAVVTVTEADARSVRDRLRAGDLPILSIPNSVPEPDVTPADTRSKIVVAAGRLATVKRYELLVRAFAKVVTERPDWSLRIYGAGGEAAALAGLIADMELHNHVFLMGTYAPLEAEWVKGSIAAVTSSKESFGMTIVEAMRCGVPVVSTACPVGPAEIIEDGVDGLLVTPGDVDAITTGLLRLITDDGLRESFGRAALRNSRRYDPAVVAGRYEELFTRLRSGGGGLSRLRPRAPRSVPRGLLRVLPGRWRPVRDHYRADCRMEGSRVVVEVPHDPAGRDLTHLVLRARGTGPERAQVRLPLAGAQPHLSASFAADEPLLAEGRWDLFAEDGRGKQHRVRAGRLDVRGLLGGCAETTPFVRNVPYPTTDGFLAVAAWQRERHAEAGQLWYGSGTVTITGRLIGATFGDAVPHLRLIRRGDRPAELTVTGWSTGSAVFSFDVDLHRLAAHRQLRLEDWDAEVSAGADGPPAPLARLLDDIVERKKTYVYPPVRVDDEVPAALYEESPTPEVSVRPYFTVQSGLAFAVSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYT9</accession>
    <name>U5VYT9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360954.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13045"/>
    <dbReference type="EnsemblBacteria" id="AGZ40895">
        <property type="protein sequence ID" value="AGZ40895"/>
        <property type="gene ID" value="AFR_13045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2649"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_2_11"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="23"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="272" mass="29655" checksum="6183C5CBCD7F0F71" modified="2014-01-22" version="1">MTTSVDLGDTEVAYTESGTGPAVIWLHGSGPGATGMSNFGGNLPAFADHRNIALDLPGWGRSPRTTDEPLLFHAAKTVSRVMTALGIERAHLVGNSYGGGVAMRLAITEPDRVGRLVLMAPGGVLPPDAPPWPVGLEHLFGYMAAEKPSREAMAEFVRLMVYDQALATDALIDERYRSSLAVHPELPIPPNFGDLTPDLPRIRARTLLIWGREDQTVPLTWAARILNDIPDAELRVLPHCRHWVQYERAAEFNHIVREFLSGGDPSATSNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYW3</accession>
    <name>U5VYW3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GHMP kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_18495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362350.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18495"/>
    <dbReference type="EnsemblBacteria" id="AGZ41977">
        <property type="protein sequence ID" value="AGZ41977"/>
        <property type="gene ID" value="AFR_18495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3753"/>
    <dbReference type="eggNOG" id="COG4542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056896_2_0_11"/>
    <dbReference type="OrthoDB" id="1240505at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012363">
        <property type="entry name" value="PduX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF1">
        <property type="entry name" value="PTHR43527:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033887">
        <property type="entry name" value="PduX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="1">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="2">
        <location>
            <begin position="211"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="302" mass="31597" checksum="D1588FC8082049D1" modified="2014-01-22" version="1">MTITSTTSLRPRSAGATGVSSAFGTFGELLQGVLPEDDGDFLVTLPVARWTMARFQETPGSDRLTVWPPHKSKALRLTEMIMADVAEPVGGLLTIDSTLPEGKGLASSSADLVATARAVGNALGVPMPPRRIESYLARIEPTDGVLYPAIVAFHHRSVRLRATLGSLPSMAVVSIDEGGAVDTVDFNRIPKPFTAADKREYAGLLAELTGAVAAQDLAAVGRITTRSAQMNQILRHKRSLPSMIEICEAVGGLGVVVGHSGTTLGILLDSADPACAGRLTAAAQACERLAGNVTVFHTLSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYW9</accession>
    <name>U5VYW9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Na(+)/H(+) antiporter NhaA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sodium/proton antiporter NhaA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nhaA</name>
        <name evidence="11" type="ORF">AFR_19455</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2 H(+)(out) + Na(+)(in) = 2 H(+)(in) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29251"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362535.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19455"/>
    <dbReference type="EnsemblBacteria" id="AGZ42163">
        <property type="protein sequence ID" value="AGZ42163"/>
        <property type="gene ID" value="AFR_19455"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19455"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3948"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015803_3_0_11"/>
    <dbReference type="OrthoDB" id="1805428at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015081">
        <property type="term" value="F:sodium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01844">
        <property type="entry name" value="NhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023171">
        <property type="entry name" value="Na/H_antiporter_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004670">
        <property type="entry name" value="NhaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30341">
        <property type="entry name" value="PTHR30341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06965">
        <property type="entry name" value="Na_H_antiport_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00773">
        <property type="entry name" value="NhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0050">Antiport</keyword>
    <keyword evidence="5 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="432"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ42163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="620" mass="66666" checksum="1BBB391A2C6E97AA" modified="2014-01-22" version="1">MQERTQWTRGLGAPLRAFLRTESGSAGVLVAAIAAALVWANVDVASYEAFWRTDLAVRIGGTGVSEDLRTWVNSGLMTIFFLVVGLEARREFDLGDLRERRRFVLPFVAGLAGMLIPVLIYLALNAGGTGAHGWGVAMSTDTALALGLLALLGKGVPDAARVFLLTVFVVDDLVALLVIAVVYSEDIEVMPLLVAFAAFGVWLLLRYLDVRRAVLYALVAIVLWAAMLMSGVDPVVAGLAMGLTAVAYTPAREKLEAASGLFKLFREQPTPELARSAALGMTQTLSPNDRLQRIYHPWSSYVIVPLFGLANAGIAIDGGFLARAFTSPVTWGVILGYLLGKPIAVVGVSALLTWLTHGRIRPGVGWAAVLGSGTIAGIGFTVSLLIAALAFEGPELAEVKIGVLTAAAGASLLTWTVFRVTSLLPADRRARALLGETEDLTDLVPAVDPDRDHVRGPADASVTVVEYGDFQCPYCGRAEPAVRDLITDTDLRYVWRHLPLTDVHPQAQLAAEAAEAAAAQGKFWEMHDVLLDHQDKLRIMDLLRYAAELGLDTNRFHDDLMDHVHVTRVAEDLESADASGVSGTPTFFINGRRHYGAYDIATLKQAVKTARARVGITRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZB9</accession>
    <name>U5VZB9_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19760"/>
    <dbReference type="EnsemblBacteria" id="AGZ42224">
        <property type="protein sequence ID" value="AGZ42224"/>
        <property type="gene ID" value="AFR_19760"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19760"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4009"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="22"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="275" mass="29300" checksum="52A7BD148244588C" modified="2014-01-22" version="1">MVPANGVDLCVETFGDRDHPALLLIGGAASAMDWWEDDFCRRLADGGRLVIRYDHRDTGRSTAYPVGYPRYTAADLVGDALGVLDALGIARAHVVGLSMGGGIAQRLAVEDPCRLLSVTLMSTSPGDVEEPAASSDAGGTPSDPVETDWSDRDAAVDRLVADVIACGGKFTADERHLRELAGRIFDRTTDMAASQTNHWLAADTGAPIRERLATITMPALVLHGTVDPFFSMAHAQALAREIPGARLVPLEDVGHENPPAAVWDVVTAEILRHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZF0</accession>
    <name>U5VZF0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase S8 and S53 subtilisin kexin sedolisin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_13945</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13945"/>
    <dbReference type="EnsemblBacteria" id="AGZ41075">
        <property type="protein sequence ID" value="AGZ41075"/>
        <property type="gene ID" value="AFR_13945"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13945"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2829"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_15_5_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase_S8" evidence="5">
        <location>
            <begin position="43"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="473" mass="47943" checksum="E7D2386FE2D2528E" modified="2014-01-22" version="1">MAFGGGWAGRWLSTATGTAAGTSLRDVRAIIGADTGAAANLTGAGVGVALLDTGVAPVDGLPAGQLVNGPDLSFESQAANLRYLDTYGHGTHMAGIIVGNDPASGSRGIAPGAKLTSVKLGTANGAVDVSQVIAAIDWVVENRNHDPANPIRVLNLSYGSGGNPSAWTDPLQFAVEQAWKAGIVVVAAAGNDGNAATMLTNPATDPFVIAVGAAATKGTLSTADDDLAPFTNSGTADKVPDVLAPGESILSLRDPGSSIDVAYPGARSGTTLFRGSGTSQAAAVTSAAAALLLQAKPWLTPDQVKSALKGGTTLATGKAGSMRLKEVNVNNALGLTPLLTSTQLYVPSNGSGNLDDARGLSRVVSNNVKLSGEFTVFGPFHSANWAAYSATGNSWVGGTWMGNPIAGNGWTGTSFASKTWGAATWLGAPWGGVQSWTDPTWSGRAWSGRAWSTGAWTGARFSSSEDWTTSLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZN9</accession>
    <name>U5VZN9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ANTAR domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_14490</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361239.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14490"/>
    <dbReference type="EnsemblBacteria" id="AGZ41180">
        <property type="protein sequence ID" value="AGZ41180"/>
        <property type="gene ID" value="AFR_14490"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14490"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2942"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074354_0_0_11"/>
    <dbReference type="OrthoDB" id="1958442at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012074">
        <property type="entry name" value="GAF_ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036625">
        <property type="entry name" value="GAF_ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ANTAR" evidence="3">
        <location>
            <begin position="168"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="235" mass="24789" checksum="CEB1AAF7F2319B99" modified="2014-01-22" version="1">MTDQYPLDPAEAFAEISRIKLSDTNLDGVLEHLARLAKRTIPGAAEVSVTLSRGKGAYTAAYTGELALALDERQYEQGHGPCLDALASGDVMSIDDMGTEERWPDFAARAIEAGPHSSLSVGLPVHESAAGALNVYAIKAHSFDDDGIALAQTFAGYAAVALANAHLYDTQASLANQMQAAMASRAVIEQAKGILMGQRRCSSAEAFAILAKLSQDTNRKLRDVAQALVDSAPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0I9</accession>
    <name>U5W0I9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Peptidase S8 and S53 subtilisin kexin sedolisin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">AFR_21720</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362986.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21720"/>
    <dbReference type="EnsemblBacteria" id="AGZ42614">
        <property type="protein sequence ID" value="AGZ42614"/>
        <property type="gene ID" value="AFR_21720"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21720"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4401"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_13_3_11"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023834">
        <property type="entry name" value="T7SS_pept_S8A_mycosin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03921">
        <property type="entry name" value="T7SS_mycosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665411" evidence="13">
        <location>
            <begin position="26"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="14">
        <location>
            <begin position="61"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AGZ42614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="404" mass="41431" checksum="255035DDF363A1B8" modified="2014-01-22" version="1">MQVRKVAVAVAVAGTVLFPAVPAQARANICQTQPGVAQVSKTQPYEDQMFAPERLAPFATGAGVRVAVIDSGVDAGHPQLRGRVAAGRDFLHGDATGRQDCIGHGTAVASIIAARPAKGAGMRGLAPAATIVPIRVSEQIDTEDAAGSSDRPVSPVKFAQAIDWAVDEGNADVINMSLVMTADDPSVRAAVARAVARGVVVVAAAGNDGKPDDANLRPYPASYPDVIGVGAIGPDGVAGDFSQHGDWVDLTAPGVDVTFAARRSGHTSGRGTSYASPFVAATAALVKQRFPSLTPAQVARRILATADPAPGGRRSDKYGVGVVNPYRALTETLGPDTPPAPAPVVMHTEDPATVALAARRAHAQDMSLLVAAIGAGVVLLVVVVAAAMRRGRRRGWRPPGPELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0M8</accession>
    <name>U5W0M8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent zinc metalloprotease FtsH</fullName>
            <ecNumber evidence="5">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsH</name>
        <name evidence="9" type="ORF">AFR_22615</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22615"/>
    <dbReference type="EnsemblBacteria" id="AGZ42793">
        <property type="protein sequence ID" value="AGZ42793"/>
        <property type="gene ID" value="AFR_22615"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22615"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4584"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_16_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="8">
        <location>
            <begin position="252"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="664"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="674"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="483"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="260"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="558"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ42793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="706" mass="74924" checksum="6B9733D3A9340A87" modified="2014-01-22" version="1">MTTPGSDQKGPLARAGLGNRAGDDRPPSPPPWRVEGAPEGGRGPDQPKRRPAWQRFGWMLLVLLALNWIISSFLLAPEPRTPVSYTFFLTQVDAKNVADITSTADTIEGTFSKKAAYTPVGEKKAEQVDRFTTQRPSFADDDLFAKLQANGVPVNANPPDAPRPIWQQVLLGFGPTLLLVGLLIWFLRRAAAGAGGGIGGFGKSRAKLYQPEGGPRTTFADVAGIEEVEQEVTEIVDFLREPEKYRKLGAQIPHGVLLSGPPGTGKTLLARAVAGEAKVPFFSMSASEFIEMIVGVGASRVRDLFEQAKKVAPSIIFIDELDAIGRARGGAQSLGGNDEREQTLNQILTEMDGFTGSEGVVVLAATNRPEILDQALLRPGRFDRRVTVSPPDLSGRRKILEVHTRGVPVGPDVDLQALASATPGMVGADLKNLVNEAALLAARRAHATVVTADFTDALEKIVLGTVRGIMLTPEEKERTAFHESGHALLGMLTPGADPVRKISIIPRGQALGVTFQSPSADRYGYSAKYLRGRIIGALGGRAAEEVVYGDMTTGAESDLDQVSNIARQMVGRWGMSEAIGPVTVLPPPGSESPFGLDGVAPATKELIDAEVRKIVDECYEEALALLRGHRDQLSALAHRLLESETLDEEDAYAAAGISRDAAPGALARGEVPGIPPAPGLPPEDPAAPADVAPREATGASKNGAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0M9</accession>
    <name>U5W0M9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_16095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16095"/>
    <dbReference type="EnsemblBacteria" id="AGZ41501">
        <property type="protein sequence ID" value="AGZ41501"/>
        <property type="gene ID" value="AFR_16095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3266"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="114" mass="13013" checksum="24E31F9BD36DF8C2" modified="2014-01-22" version="1">MTTDVLPRVFAALADPTRLDMVARLSEGDATASQLAEPYRMTLQAVYKHLRVLEEAGLVSRPPGPQPRPVRLEVEVFDLMNAWIERYRVRAEQRYRRLDAVLAEMEESGDAQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0T1</accession>
    <name>U5W0T1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_21710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362984.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21710"/>
    <dbReference type="EnsemblBacteria" id="AGZ42612">
        <property type="protein sequence ID" value="AGZ42612"/>
        <property type="gene ID" value="AFR_21710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4399"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113685_0_0_11"/>
    <dbReference type="OrthoDB" id="1358723at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="178" mass="19655" checksum="57955E8995D6099F" modified="2014-01-22" version="1">MTTEDTVNAWLRERGAETIDHPGGTLYLHLGRVHDRLGALGHDPGVQLAGLAHAVYGTDGFDLTLLDVADRGTLRDLVGERAETLVYLYGACDRSRTWKTLPETRQVWNRFTGTAEDLPDDLVQPFTDLTIVNELDVAEQEPSIAARYGDYFRTLFGSWAPLASAPVLAESRRVLGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1J4</accession>
    <name>U5W1J4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phytochrome A type 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_24000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24000"/>
    <dbReference type="EnsemblBacteria" id="AGZ43068">
        <property type="protein sequence ID" value="AGZ43068"/>
        <property type="gene ID" value="AFR_24000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4862"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_44_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="323"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="536" mass="57549" checksum="BE7F277624A1272D" modified="2014-01-22" version="1">MGGDEQQRLAALHEYRMLDAPADDELEAVVRVAAMVAGVPTATLNLIDEHRQCQLTSTGFEGGISPRSDSMCAVRFRQGEFVWVRDASTDPVYRANPWVTGVLADVRFYASAPLVTPQGHALGTLCVFDSEPRDLTGEQIARLEDLAQVILALFERRRQARINGELAAEAEARQQFTDAVLETIDVAVVAADPAGHLALFNRAARDWHGLDADPSVAPGDLPDTYRLFRTDGVTPLPGDEVPLMRALHGGAVSGAEMVIRSPGGQARHVVAGGRALTAADGSVAGAVVAMSDVTADRAQRHALEQAHAELLRSNGELEQFAAVVGHDLAAPLGVVNGYLELLGDDYDAVLDDRARKWINSATRAVARMQGLIEAMITYARAGSEPCRRQLADLGDVVDQALMDLRDRVRESGATITVPALPTLACDPTLLRQLLQNLIGNGIKYRDPGRPCHIGVGAELRDDEWLVTVTDNGLGIPADQRRRVFEMFARADPHHRAGHGIGLSTCQRIVERHGGRIWAGAAPGGTGTTISFTLPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1V2</accession>
    <name>U5W1V2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_24055</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24055"/>
    <dbReference type="EnsemblBacteria" id="AGZ43079">
        <property type="protein sequence ID" value="AGZ43079"/>
        <property type="gene ID" value="AFR_24055"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24055"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4873"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="5"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="242" mass="25076" checksum="698E58D71E78ED13" modified="2014-01-22" version="1">MGLVGPNGAGKSTLLGLTCGLVAPTTGSIEVLGSRPAAGAAQLARVGFVAQDAPVYGSLSVADHLRLGARLNPRWDRPLAERRVQRLGLDPAQKAGRLSGGQRAQLALTLAAAKRAELLILDEPVASLDPLARRAFLLDLLDFVAELGVSVVLSSHLLGDLEQVCDHLVVLAEGRVQLAGDTADLLAVHHRVTGDLPPGAERIHEDVVRSEVPVPGASPVDLEDLGMAYMTRAAVVSVGGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W221</accession>
    <name>U5W221_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">B12 binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_18515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362354.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18515"/>
    <dbReference type="EnsemblBacteria" id="AGZ41981">
        <property type="protein sequence ID" value="AGZ41981"/>
        <property type="gene ID" value="AFR_18515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3757"/>
    <dbReference type="eggNOG" id="COG2185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122414_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="1">
        <location>
            <begin position="9"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="15479" checksum="31290FF11F623B92" modified="2014-01-22" version="1">MNLTYAIPGPSILVTGTSSDSHTWNLVYLQLMLEEMGCRVHNLGACVPPALVAAECLGDNPDLVVVSSVNGHGFHDGLRLIEELRARPELAGTPVVIGGKLSTDGLRNVGLVRRLRAAGYDAVFENGDLTRFRTLVGRLSARVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2J7</accession>
    <name>U5W2J7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-hydroxybenzoate 3-monooxygenase</fullName>
            <ecNumber evidence="2">1.14.13.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.2" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362503.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19295"/>
    <dbReference type="EnsemblBacteria" id="AGZ42131">
        <property type="protein sequence ID" value="AGZ42131"/>
        <property type="gene ID" value="AFR_19295"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19295"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3919"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057691_0_0_11"/>
    <dbReference type="OrthoDB" id="1770293at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018659">
        <property type="term" value="F:4-hydroxybenzoate 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="2"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="379" mass="41509" checksum="63B12C34347D03CA" modified="2014-01-22" version="1">MRTQVGIVGAGPAGLVLALLLDRAGIDAVVLERRSRSYVESRVRAGVLEHGTADLLDELGVGARMRREGMVHRGIELRFDGTGHRIDLEGLTGRTITVYGQQEVVKDLIAARTGAGPLHFEAEVTGVDGTTITYKHNGVEQRLECDFVAGCDGSHGVARTAIPPEALTVAERVHPYAWLGILARAPAAADELVYAYHERGFALHSMRTPGIVRLYLQVEPGTDLADWPDSRIWDELRTRLGLPLQEGEVLERGVTPMRSVAVEPMRHDRLFLAGDAAHIVPPTGAKGLNLAVSDVRVLAEALTAYYRDSSETLLDAYSEICLKRVWQVQRFSNWMTTMLHRRPGDDHFGHRVQLAELAYVTGSTAAATTLAENYVGLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2P0</accession>
    <name>U5W2P0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_19520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362548.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19520"/>
    <dbReference type="EnsemblBacteria" id="AGZ42176">
        <property type="protein sequence ID" value="AGZ42176"/>
        <property type="gene ID" value="AFR_19520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3961"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_2_3_11"/>
    <dbReference type="OrthoDB" id="550338at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="270"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="393" mass="40544" checksum="D6CF14E0C66038E4" modified="2014-01-22" version="1">MRDAVIVEAVRTPVAKGKPGGAYADIHPVDLHAHVLRAAVDRLGLDPAEVDDVIGGTVGQVGEQSTNNTRLAVLAAGFPESVPGTTVDRQCGSSQQALSFAAQGVAAGAYDIVIASGMESMSRVPIGSSALAGGTRAFVGGPAVAARYPGGLVPQGVSAELIARRWGLTRVQLDEFAATSHQRAAEAWKQGRFAEQIVPMAGLETDETIRPGTTVETLAGLRTAFADERWEQRFGAIDWKVTAGNSSPVNDGAAALVVTTSEIARQRGWRPRARVHTATAVGDDPILMLTGIIPATAKVLARAGLTLADIDAFEVNEAFSSVVLAWLAETGADPAKVNMDGGAIAIGHPLGASGARLATTLLGVLDRTGGRYGLQTMCEAGGTANATVIERLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2U5</accession>
    <name>U5W2U5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43337">
        <property type="protein sequence ID" value="AGZ43337"/>
        <property type="gene ID" value="AFR_25355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5138"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="5"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="259" mass="28495" checksum="9F7D980D8F1C0772" modified="2014-01-22" version="1">MRRGGHRDQVFLATKGSALPRPSPNLWSADGTPDWNLARRTFEGAGADTLRHAVDASLQRLGTDHIDLYYVHVDDLTTPLSETLEALAGLVAAGKIRYLGWSNVRTWRLERIRQLCDRHGWPAPVAVQQQHSYLRPNPATDNASITGHEQLDYLAAHDDLTLVAYSPILKGIYDDPARRAAHGVMESYAGPDTDARLALLTEMAADLGVTPGQLVLAWLLHSTPRVIPLIGPRTPEQLESALQALDVKLTEDQLQRLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2U7</accession>
    <name>U5W2U7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SARP family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_25950</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AfsR/DnrI/RedD regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25950"/>
    <dbReference type="EnsemblBacteria" id="AGZ43454">
        <property type="protein sequence ID" value="AGZ43454"/>
        <property type="gene ID" value="AFR_25950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5261"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004665_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005158">
        <property type="entry name" value="BTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03704">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01043">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="8"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="60"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="60"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="985" mass="104039" checksum="378BD4616AF6C2A6" modified="2014-01-22" version="1">MPSLGATLREYRQRAGLTQDEAAAAAGISVRTLRDLEQDRNARPRRRSVAALAEVLRMPAGSWERQGGPLSIGILGPLLVRHGDTDVEPAGALPRRLLGLLALHHGEAVGRDRIVDVLWPEATPASSQNLMHSYVATVRRWLEPGRAAGAYSVLVRAGDGYRLTAGDELDLALFRAGVRAAREAVERQEHAVADGHLRTALGLWRGRLLGDADPVLGGLGAAAAVAAERLEATLLYADLAATRGEPGRAAAALQAIAEDEPLHEGLHVRLITTLAASGAQAAALRAYEGIRGRLADELGVDPTAELRAAYQEVLRGRSDGRPVPAQLPPGGRYFTGRGGELARLDAVADTLDPGGTAVVISAVTGAAGVGKTALALHWAHRSAARFTDGQLYVNLRGFDPAGPADPADALRALLEALGVPAGDLPPTVADRSSLLRSLLARRRILLLLDNARDAEQVRPLLPGAAGCLALVTSRDRMTGLVVTDGAVPVALDLLSTGEARELLARRLGAARVAADEEATLEIIERCGYLPLALAVVAARAATQPGLELSALAAELRAARLDALDGGDLPSDVRAVFSWTFEALPPAARDLFPLLAIHPGTDLSLAAAASLAGRDEKSTAEVLAELVRVSLLEESGPGRFRLHDLLHHYALEQAAGTEVRQAAFERLVDHYGRAAHAADRLMFPGRQSFELPAPLDGVSVPELSGPEDAAAWAAAELPVLGPVTRLAAEAGLDDRAWQLSWSATMMRHMRNEFAAQAEVLTTAIGSAERLGDLRRQAISHLDLGMTFSHLHRYDDAAAELRRALSIAEAGGDSRTQGRAYLNLGQVLDRTGDPRAALVEAEKALVLFRGGGDRIGEGHALNAVGWLATQIGDHQRGLAAAQDSLALHEAAGDAYGTAAALDSVGWAHFHLGEYAEARDCFKRALPFCLDQGDHLLSAVVLEHLADTEHAAGDQAAARDALNEAIGHLEEIDPAQAAKLREKGYSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2W0</accession>
    <name>U5W2W0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integral membrane sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362628.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19920"/>
    <dbReference type="EnsemblBacteria" id="AGZ42256">
        <property type="protein sequence ID" value="AGZ42256"/>
        <property type="gene ID" value="AFR_19920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4042"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_2_11"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025828">
        <property type="entry name" value="Put_sensor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13796">
        <property type="entry name" value="Sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="2">
        <location>
            <begin position="320"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="410" mass="43109" checksum="878546EF58ABDE94" modified="2014-01-22" version="1">MEAKRLREARATAWRAFVELLGGLGTACLALAVLAWLVLVALTSVIGIGLLLAPSALRLLHAVADVERRRLSRRGAEVLAPGPLPTALREAAADPATRREVLWVAAHATGGLLLGVIGIQLPAWALRDFTFPLWWRMVPEGDATPGIGLWTVHDWPGVATVTLLGVVWAVMTVGLAPGMARLQSSWGRRLLAPGPETDLSLRIAELTSTRAAALDAHAVELRRIERSLHDGTQNRLVATTVLLGAARRMLTRDPAEAEVLLERAQGAAEQALAELRAVARGILPPVLADRGLAGALSGLASGSPVPCQVEVDVPARLAASVESTAYFVVAEALTNVAKHSGATHVEVDVRSRGPRLHVRITDDGRGGADEDGGSGLVGIRRRVQALDGTLTLTSPPGGPTTLEVELPCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W373</accession>
    <name>U5W373_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B12-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364578.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26985"/>
    <dbReference type="EnsemblBacteria" id="AGZ43658">
        <property type="protein sequence ID" value="AGZ43658"/>
        <property type="gene ID" value="AFR_26985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5469"/>
    <dbReference type="eggNOG" id="COG5012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069063_0_0_11"/>
    <dbReference type="OrthoDB" id="927390at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="1">
        <location>
            <begin position="100"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="347" mass="37461" checksum="8A2FE6CAE7755459" modified="2014-01-22" version="1">MTVFTTTPPRSLDDPGLGEEYLRLVGDGDEYGAVEVVNELLENGTPPQRVMLDLIARTQGRVGELWAANEWSIAREHAATAISERALAAVAARSSVRPTRGRITVACVDGEWHALPVRILGEMLRLDGWRVDFLGANVPGTHLVTHLHQTGPDAVALSCMIATRLPRAHAAITACRSVGVPVIAGGRGFGPDGRWAVKLGTDAWAAGAQEALDRLNGDWPPRIGDPYETAFLGDDEYTYVVRHRGDLVSTALVRLAAAYPPVSGYDQRQHDATAEDLGHVVDFLAAALYVDDAQIFTDFVQWTSAVLEAREVPPVALQIGLMLVRDQLRDYPAAIAVIDAGVAKLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3C8</accession>
    <name>U5W3C8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Saccharopine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364305.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26300"/>
    <dbReference type="EnsemblBacteria" id="AGZ43522">
        <property type="protein sequence ID" value="AGZ43522"/>
        <property type="gene ID" value="AFR_26300"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26300"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5331"/>
    <dbReference type="eggNOG" id="COG3268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_810944_0_0_11"/>
    <dbReference type="OrthoDB" id="1006830at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sacchrp_dh_NADP" evidence="1">
        <location>
            <begin position="37"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="31791" checksum="75CF0ECD8F258FA8" modified="2014-01-22" version="1">MNKRIAVLGATGHTGGFVVAELRSRGVTSIPCGRGTDLDAAFRDADAVINCAGPFAATAEPVIRAAIRAGIAYLDVTAELEVVADTFATFAGTEIPIVPAVAFFGGLGDLLATSAMGDWKSADQVTIAYALSSWRPTPGTRATGRVSASRRNGRRIVFAAHELHEMTGDAPRSKWTFVEPVGTREVVGEFTTADSATIPSHLDVREITTVMSANAVDDLHGPDPQGPVAVDGLGRSAQTFLVEVVARSSTEERRAVASGHDIYAITAPLVVEAALRELAPGVACVGARFDAAAFLEELPLTPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3P3</accession>
    <name>U5W3P3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycerol-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="6">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_27465</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a quinone + sn-glycerol 3-phosphate = a quinol + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560092.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27465"/>
    <dbReference type="EnsemblBacteria" id="AGZ43754">
        <property type="protein sequence ID" value="AGZ43754"/>
        <property type="gene ID" value="AFR_27465"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27465"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5567"/>
    <dbReference type="eggNOG" id="COG0578">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015740_5_1_11"/>
    <dbReference type="OrthoDB" id="360065at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052591">
        <property type="term" value="F:sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="PTHR11985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00977">
        <property type="entry name" value="FAD_G3PDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="8">
        <location>
            <begin position="24"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="DAO_C" evidence="9">
        <location>
            <begin position="401"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="538"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ43754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="568" mass="61575" checksum="7AE27C79259BB5D0" modified="2014-01-22" version="1">MTDTTLSPAAREAALSALAGNEVDVLVVGGGVVGAGSALDAATRGLSVGLLEARDFASGTSSRSSKLIHGGLRYLEMLDFALVREALRERGLILTRLAPHLARPVPFLYPLQHRGWERLYAGAGVLLYDTMAAAAPGLPRHRHLSRRGALRVAPALRRDALVGALQYYDAQLDDARHTMFLARTAAAYGAHVASRAEVVGFLREGERVTGVRVHDLEHDKTFEVRAQQVINATGVWTDDTQALVGERGQFHVRASKGIHLVVPRDRIQSATGIISRTPSSVLFIIPWGRHWIVGTTDTDWTLDKAHPAASSTDIDYLLDQVNKVLATPLTRTDVQGVYAGLRPLLSGESESTSKLSREHTVGSPTPGLVVVAGGKYTTYRVMARDAVDAAVHGLGRSVPRSCTERVPLLGADGYQALWNRRRVLADESGLHVARVEHLLGRYGSLIHELLDLIRADPGLGKPVDGADDHLRAELVYAASHEGARHLEDVLTRRTRISIETFDRGLAAADVAADLMGSVLGWSPDQTDREVRNYRLRVEAERASQEQPDDETADAARLGAPEVVPLRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3Q6</accession>
    <name>U5W3Q6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Class III aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27540"/>
    <dbReference type="EnsemblBacteria" id="AGZ43769">
        <property type="protein sequence ID" value="AGZ43769"/>
        <property type="gene ID" value="AFR_27540"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27540"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5582"/>
    <dbReference type="eggNOG" id="COG0160">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="433" mass="46117" checksum="FC6C44F011ACDE27" modified="2014-01-22" version="1">MTDDLLARHQAVMPSWVTTYYGDNAIELVSGSGRRVTDAAGRTYLDFFGGVLTNMLGYDIAEVREAVERQLATGIVHSSTLYLIRQQVELAEKIARVSGIPDARVFFTNSGTEANEAALLFTTNHRRSNQILAIRNSYHGRSFAAMAITGHRSWSASALSPVSVSWLNSGERLRGRMKGLSDADHIDAAVEDLREVLATTTSGDVAALIAEPIQGVGGFVHAPDGLLGALKKELDNHGILLIADEVQTGWGRTGDHFWGYQAHDVQPDLITFAKGIGNGFALAGVVGRAELVNAVPATSFSTFGGNPISTAAGNAVLDYVLDHDLQANAARTGTILLDGLRAALGDTKIVGEIRGRGLMIGIEFVRPGTTDPDTAATMRVFDECRKGGLLVGKGGLYGNVLRMGPPLTLTEDEAREGLAILTEAIRTADAESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W3W0</accession>
    <name>U5W3W0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fibronectin type III domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_28210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28210"/>
    <dbReference type="EnsemblBacteria" id="AGZ43903">
        <property type="protein sequence ID" value="AGZ43903"/>
        <property type="gene ID" value="AFR_28210"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28210"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5718"/>
    <dbReference type="eggNOG" id="COG4733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_437983_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="407"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="342"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="599" mass="61463" checksum="B9840BABF08FBA3D" modified="2014-01-22" version="1">MAVTAAVSGASSATPGVRFAQAGRWFASPADDLIYHVNGSARTVDAQTRIEGLEPGSEVVQGETSGYVIGSSRITEFGKSTLSVEQTLTAPAGERPVALEAKGGPYLVYRETGTVVRLGPEPKTIPAGQPLGEPVVTPDGTLWLHRTDTNVLCRLRPDSDVLSCPASAPVGHTGALTVADDRPVFVDTSTDTLSPVTGDGLGPSTSIGVDLPADAEVAAADVRGRLAVLDPRQHRMHLVDITAAAAPVTVDLPAGTYAPPSAGRSSVVLLDVERRSVLTYDSDGRRQSVTPVPPESGDPRLSRGEDDRVYVEGGEGKQVLVVDDRGRAGAVTPVAAAAPSPSSNRPQPPSAGPTRPVPPVRPPSIPPSIPPSRGTSGPTGPVATSPVTTATGPATNPPARKVVPASPPGMPPGVRASARGKDIVVSWSAAAANGAAVSGYRVSWAPASGGAARSQDRAGGARSATLTGATVGVAYRITVAASNSAGRGPAATVRATVPVPRPVRSVVVSRGETTTYEHGCEPPECAFIKVTMTGFKPNSAYQITPHASTYGDFNPGAELTTDDKGNLTARHRFPFNGVGQRVWVTVDGLTSNRLLWTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4V2</accession>
    <name>U5W4V2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase molybdopterin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_23480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23480"/>
    <dbReference type="EnsemblBacteria" id="AGZ42966">
        <property type="protein sequence ID" value="AGZ42966"/>
        <property type="gene ID" value="AFR_23480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4758"/>
    <dbReference type="eggNOG" id="COG2041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040538_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="SSF56524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Oxidored_molyb" evidence="2">
        <location>
            <begin position="277"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="411" mass="43920" checksum="C2571B1EA361B5B1" modified="2014-01-22" version="1">MVRNTDPGLKRSIVARVVDWVEAPLPQPPAALRRGPLQDGAFDSKLRSPRLTSILGIALAVAFSICLVTGLISHFIQHPPAWFWWPSRPAGLYRVTQGLHVATGLASVPLLGAKLWSVYPRLFGWPPARNLAHAAERASIAVLVAASLFQVLSGILNISRWYAPMPFFFTAGHYWAAWLATGALVTHIGVKLPIIRQALVRATPDRTAPGALSRRGLLGTVGAAAGVITLATVGQTVAPLAPVSVLAPRRPRTGPQHLPVNKTAAGAGVRDAAFDPAYRLTIAGPGKQVSLSLAELGALPQHTVILPISCVEGWSSTATWTGVRLRDLVVLVGGDPQDAEAYVESLEARGRYRAATVPPTHTRDPFTLIALRLGGEALHPDHGYPARLIAPNRPGVLQTKWVTRITVRSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W510</accession>
    <name>U5W510_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_23815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_23815"/>
    <dbReference type="EnsemblBacteria" id="AGZ43031">
        <property type="protein sequence ID" value="AGZ43031"/>
        <property type="gene ID" value="AFR_23815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_23815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4824"/>
    <dbReference type="HOGENOM" id="CLU_2379741_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="9849" checksum="1AC0EB9E0B74751A" modified="2014-01-22" version="1">MLGAMTGEHDDLRTIRLRAANLSMGIGLLGLLLAFFYLSSPVLPAFILLVLGGVVAIGVYRARDTSMSRWFTVWSLLLTVAGVGAAALIGSLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W513</accession>
    <name>U5W513_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="7">Glutamate N-acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">Ornithine acetyltransferase</fullName>
                <shortName evidence="7">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="7">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="7">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">N-acetylglutamate synthase</fullName>
                <shortName evidence="7">AGSase</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">argJ</name>
        <name evidence="8" type="ORF">AFR_30240</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="7"/>
    <dbReference type="EC" id="2.3.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560645.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30240"/>
    <dbReference type="EnsemblBacteria" id="AGZ44308">
        <property type="protein sequence ID" value="AGZ44308"/>
        <property type="gene ID" value="AFR_30240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6123"/>
    <dbReference type="eggNOG" id="COG1364">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027172_2_0_11"/>
    <dbReference type="OrthoDB" id="1083409at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:acetyl-CoA:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103045">
        <property type="term" value="F:methione N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="PTHR23100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5023379065" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="7">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023379063" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="7">
        <location>
            <begin position="186"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="7">
        <location>
            <begin position="185"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="390" mass="39492" checksum="470DFE3DDB801E20" modified="2014-01-22" version="1">MTVTTPRGFRASGVAAGLKTSGGADVAIVVNDGPDATVAGVFTGNRVKAAPVMWSQQVLKGGVVRAVVLNSGGANACTGTQGFRDTHATAEHAAAVLRGGPKPLLVGAGDIAVCSTGLIGEMLPMGKLLPGVTAAVKALNTDGGARAAEAIMTTDTVAKNAVVQGDGWSVGGMAKGAGMLAPALATMLVVLTTDAVAGSEALDTALREATRLTFDRIDADGCMSTNDTVLLLASGASDVQPTQEELTAAVTALCHDLAQQLIDDAEGATKHIAIEVLGAASEADAVEVGRTVARNNLVKTALFGNDPNWGRILAAVGTTQAAFAPDRIDVAVNGVWVCRGGSAADDRSKVDLAGKDVTITVHLHEGEMSATIWTTDLSHAYVHENSAYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W588</accession>
    <name>U5W588_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP/GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560514.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29580"/>
    <dbReference type="EnsemblBacteria" id="AGZ44177">
        <property type="protein sequence ID" value="AGZ44177"/>
        <property type="gene ID" value="AFR_29580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5996"/>
    <dbReference type="eggNOG" id="COG1474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050343_0_0_11"/>
    <dbReference type="OrthoDB" id="453710at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021228">
        <property type="entry name" value="BrxD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10923">
        <property type="entry name" value="DUF2791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="437" mass="47310" checksum="14B58880E959670E" modified="2014-01-22" version="1">MSGVSPLRRREVIDALRRGAVPSAGLDLLAVGLDRFHRAIDEDLDAVAASGSVFKAVRGEYGSGKTFFVRWIAERAKRANLATAEVQISENETPLHRLETVYRRLTERLTTTTFPPSALRPVVDGWFYALEEDVLAAGKVDSDDAAVLDRAVGVLLDQRLAEVSRSAPAFAAALRGYRTAGLSGDAATADAVLAWLGGQPHVAAAARRVAGVKGNLDHFAALSFLQGLLAVLRDSGHPGLLLVLDEVETLQRVRSDARDKALNALRQLIDEVYSGRFPGLYLIITGTPAFYDGQQGVQRLAPLAQRLAVDFDTDPRFDNPRAVQIRLPGFTVDSLVELGGRVRDLYADGSSAAERVRDLVDDAYLDKLARAVAGGLGGKVGIVPRLYLKKLVSDVLDRVDQFPDFDPRQHYAPTMSTAEMTDVERNAASADEIALDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W599</accession>
    <name>U5W599_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_29630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RsuA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560524.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29630"/>
    <dbReference type="EnsemblBacteria" id="AGZ44187">
        <property type="protein sequence ID" value="AGZ44187"/>
        <property type="gene ID" value="AFR_29630"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29630"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6006"/>
    <dbReference type="eggNOG" id="COG1187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024979_1_2_11"/>
    <dbReference type="OrthoDB" id="1037615at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018496">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01149">
        <property type="entry name" value="PSI_RSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="10"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="246" mass="26691" checksum="9253FE417CAAA25A" modified="2014-01-22" version="1">MPQADQDTSIRLQKILATAGVGSRRGCEDLIFRGRVTVNGKVAKLGDKVDPATAEIHVDGSRIVTDTKLVYLAMNKPRGVVSTMDDEKGRTELADFLGTFEQRIFHVGRLDADSEGLLLLTNDGALAHKLMHPSYGIAKTYLAEVAGPLPRNVGRRLLGGIELEDGPAKVDAFKLIDAIGKTAQIELVLHEGRKHIVRRMMDEVGHPVSRLIRTAVGPIRLGDMRPGGFRRLSNAEVGALFKAVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5D0</accession>
    <name>U5W5D0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HhH-GPD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_29780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560554.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29780"/>
    <dbReference type="EnsemblBacteria" id="AGZ44217">
        <property type="protein sequence ID" value="AGZ44217"/>
        <property type="gene ID" value="AFR_29780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6034"/>
    <dbReference type="eggNOG" id="COG0122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_72_3_11"/>
    <dbReference type="OrthoDB" id="1781735at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="1">
        <location>
            <begin position="126"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="32670" checksum="ABFC212466D61CB1" modified="2014-01-22" version="1">MTFTLRARGPFSLAASARFSEEFPAGQGGGEDARLDLAFPADGSWTPVGVRVNADLGVDVVANPGGVPVSRIRGQVERILALDVDGSGFAAIGSRDPVVAALQARFPGLRPVQFHSPYEAAAWSIIGQRIRMTQAAGIRTRLAQQLGDSIDFGDRQLPSFPSPERLAALDGFPGLTERKVTQLQALGRAAADGQLDAADLRSMPPEEALVQLQKLPGVGPFAAELILLRGAGHPDGFPVTEKRLHRAMAAVYHLGDDPDLATLQKIALGWQPYRTWVALLLQVWLEDVTHEIAGGRRVDVLPDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6I1</accession>
    <name>U5W6I1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31735"/>
    <dbReference type="EnsemblBacteria" id="AGZ44607">
        <property type="protein sequence ID" value="AGZ44607"/>
        <property type="gene ID" value="AFR_31735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6424"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013364_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="38"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="443" mass="47687" checksum="A0432E16EC4AE490" modified="2014-01-22" version="1">MQCASLKVPVDWAAPYGPSIQVAVARRPAADPKNRIGTLLVNPGGPGGSGVDFTLDSTSYFSPELRKRFDIVGFDPRGVSRSNPIVCTTTLLTAAPPITIDTAAQYTTAVAYNRRLADDCAEQTGPLFGHVDTLSIVRDTEALRAALGEDKISFYGASYGTLLGEQYAARYPQRIRALVLDSVMDHSRDIDGFLSAEALAVQGSFDQFVRWCDRSTECAVHGRDVRKLWDGLLASARRGTLKDPYSPTYKLSVYELTNVAFGSFYDPQWHSLGIFIKEAVAGVAGKRARAVVDVSEHIFPAAFCDDWVVPVTGYADLRSRMARAAGRAPQMGFSPLALSSVNGCLGWTRGTSNPQVRLPTARTGPVLLVNARYDPATPHVWARNVAAQLGPKASLLTYEGWGHTVYGRSDCINAVVDRYLLTLKPAATGASCPAIEPKAAGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6J1</accession>
    <name>U5W6J1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">dTDP-glucose 4,6-dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_26395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364343.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26395"/>
    <dbReference type="EnsemblBacteria" id="AGZ43541">
        <property type="protein sequence ID" value="AGZ43541"/>
        <property type="gene ID" value="AFR_26395"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26395"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5350"/>
    <dbReference type="eggNOG" id="COG1088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_14_11"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="4"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="304" mass="32764" checksum="97764B8E86E0ED15" modified="2014-01-22" version="1">MNLLVTGGAGFIGSCFVRTVLADALPGLEDARITVFDKLTEAGHFANLGPVAESKNLDFVPGDICDEPLVNSVLPRHDAVVNFAAEPQNSPDFTATNVLGTQVLLDAALRHGVQRFVQVSTAEVYGPPGTGSRLAPHSPYAATKAAADLLALAFHRTHGLPVVVIRGAGTYGPYQHPSEIIPRSVTDLLEGRPAPLNDGQPREWLHVTDHCRATALVLLGGTAGEIYHVGGTQALLADRLLDAFATDTDQSTEVADQPLPHTLDDSKIREELGYRPQVDFTTGLADTIQWYRNHEDWWRPLTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6N2</accession>
    <name>U5W6N2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkanesulfonate monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561152.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32780"/>
    <dbReference type="EnsemblBacteria" id="AGZ44814">
        <property type="protein sequence ID" value="AGZ44814"/>
        <property type="gene ID" value="AFR_32780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6634"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_1_0_11"/>
    <dbReference type="OrthoDB" id="919913at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="353" mass="37894" checksum="0BF50908BFC87B6A" modified="2014-01-22" version="1">MQIHWFLPTAGDSHGITSGGQAAATVVTERPPDLGYLAILARAAERLGFTGMLTPTGTFCEDAWITTAALLAETSTLKFLVAFRPGLVSPTLAAQMASTYQRISGGRLLLNVVTGGESTEQRRFGDWLDHDQRYARTAEFLQVLRGAWSGEPFDFTGEHYRIEGATTRLTPDPVPPIFFGGSSPPALRVAAAHADTYLTWGEPPDAVSAKLAAVRALTDRPLTYGIRLHVITRDTAAEAWAAARALLDRVPPQAVEAARERFARTESEGQRRMAELTGGDSLEVSPNLWAGFGLVRPGAGTALVGSHAEVADRIAEYHALGIDHFILSGQPHLEEAYRFGEGVLPLLKLGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6U6</accession>
    <name>U5W6U6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_27220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560043.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27220"/>
    <dbReference type="EnsemblBacteria" id="AGZ43705">
        <property type="protein sequence ID" value="AGZ43705"/>
        <property type="gene ID" value="AFR_27220"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27220"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5516"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="167"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ43705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="36558" checksum="BE48E218A65C0580" modified="2014-01-22" version="1">MGVDTAVAVGVAAVLAVVVSADQGGGIGAAAYLWAAALGALMFWRRRYARTVLALTVLGLFAYYAAGFPAIGVAVPVAAALYAAAEAGRRLAAGVAATTVLAVSVTFRLIEGEDARYLVLYDAVGHAALMAAAIVLGELVRARRRIVALTDRQIALEADRRLAVHKQALARELHDAIGHTLTVAAIHTSVARQEVSRGPAKAEAALDHVSQAVSQALTELRATVRELRVPSLDDVDSLAETARAAGFEVRTRIEAVDAPELSAAAYRLVQEAVTNALRHSAGRVIEVDVRHAGADLLVRVHDDGTPAGPAAENAGLRGLRDRVAALGGRLDAGPGERGWQVSAALPLGVGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5W896</accession>
    <name>U5W896_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_29425</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560483.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29425"/>
    <dbReference type="EnsemblBacteria" id="AGZ44146">
        <property type="protein sequence ID" value="AGZ44146"/>
        <property type="gene ID" value="AFR_29425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5964"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_11"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="323"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="381"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ44146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="597" mass="64168" checksum="5B2826B46AA028FE" modified="2014-01-22" version="1">MREHRVPLRRSLIIRLLATSVLVAICATVSTAWLVVQSTTQAVRQEQGRSLSDDTSVYDQLLGYAATHPDWSTVDALVDSKARELGRRITLTTEERQVLADSAGTGGPSLGTARPSAIVDPLSVDLKLTGGTQRIDPRAVGPYRLPSRERQELRAVAEQRAACLKREGMAAEVVETASGRPILRLPASAPRAADLNCTVGTLDETRTEQKALRKLLAMTMACLGGDPSESKLAIEPDFTTIFGEGDKPGSVARARQVNACVLKSRQTQLQPYVAPPALLFVTDPADSTAQPTFNLSQDNIIRIATVTGAVLLLAILITVLVGARLVRPLRRLAEAARRPVTAQVRVPVTTRDEIGYLATALNDLSERRERTEQQRKAMVNDVAHELRTPLTNMRSWLEAAQDGLAPAQPQLVDLLLKETVQLQHIIDDLRDLAAADADNLRMHPETVYLNVTLEQVAEAHRGAAEAAQVRVVTEAAGDPQVVVDPVRLRQLVGNLMSNAIRHTPAGGTVTVRSRVAGGQLTIEVTDTGSGIAAEDLPKIFDRFWRADSSRTRSTGGSGLGLAIARKLATAHGGTLSASSTAGSGSTFTVHIPVTPTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8G5</accession>
    <name>U5W8G5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GDE_N_bis domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_35890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561770.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35890"/>
    <dbReference type="EnsemblBacteria" id="AGZ45434">
        <property type="protein sequence ID" value="AGZ45434"/>
        <property type="gene ID" value="AFR_35890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7264"/>
    <dbReference type="eggNOG" id="COG3408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019216_1_0_11"/>
    <dbReference type="OrthoDB" id="70058at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032856">
        <property type="entry name" value="GDE_N_bis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14742">
        <property type="entry name" value="GDE_N_bis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDE_N_bis" evidence="1">
        <location>
            <begin position="18"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="673" mass="70490" checksum="75EB825974B9182B" modified="2014-01-22" version="1">MNRLQPLLHDLVATVAAPTSALAGPDGQVRTAGVQGVFHADARVLSEAVLRLDDRETEPIGHADAGAGTTHFVSLARWLGDPGPDPTVRVQRTRIITPGLLEERITIASGAAGQVSTTVTVDLAVDLAPIEVVKSGSRGTPIAAHRSADGTLTWERDGITVTVTAPEAETDNHGRLTWAVTLGPGADQTLTFSVRVNDPSAVVHAPATARTWSPAEVRSGDQRLGRLVARSLDDLHALRLADGDDVYLGAGVPWFLTLFGRDSIWAARMLLPLGTDVAAGTLRVLARRQGTRTDPNSGEAPGKILHELRRQEFVLESSGLHLPAAYYGTIDATPLWISLLHDAWRWGLPEAGAGELLPHLQAALSWLDGPADPDGDGFLEYLDTTGRGLANQGWKDSGDAVRFHDGRLAEPPIALAEVQGYAYRAALDGAALLDAFGQPGADRRRDYAGTLAERFRAKFWVDGGYPALALDRNKRPVDSLTSNIGHLLGTGLLTEAEERAVATALGGDTMSGGYGLRTMSAGDAGFTPLSYHCGSVWTHDTAIVAAGLARAGFAAEATTLIDGLLTAGEAFGYRLPELHAGDARSALSRPMPYPAACRPQAWSAAAAVLMLQATLGLQPDVPNGRVRLDPLAGAPFGPITATGLRIAGHAVDVTVDAAGAATISGLPAGLTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8J6</accession>
    <name>U5W8J6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative PfkB-family carbohydrate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_35355</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561663.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35355"/>
    <dbReference type="EnsemblBacteria" id="AGZ45327">
        <property type="protein sequence ID" value="AGZ45327"/>
        <property type="gene ID" value="AFR_35355"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35355"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7154"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_6_0_11"/>
    <dbReference type="OrthoDB" id="1604782at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="4">
        <location>
            <begin position="12"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="327" mass="32064" checksum="A5A5C4BABE266C3C" modified="2014-01-22" version="1">MTTGQPAPGRKGVLVVGDLVTDVLVVHVGSLAVGSDTDARITVGGGGQAANTAAWLAQAEAQAGAGPDARGQAEDESGGGEGGGVTLIAATGDDHAGRERIAELLAAGVRCAVRAHPGVPTGSVVVLSSTGERTMITDRGAALLLDPADVTAAVKTAHDASHLHLSGYPLLHAGSRPGGLAALAAAAERGLTTSVDAASAEPLRQLGAEVFLDLVRGTDLLLCNADEAAVLAGAGTAEEQSARLARSARNVVVKLGADGAVWRGEDGLLRLIGGVRVPVIDPTGAGDAFAAGLLRAWCSGAGPQAALEAGAALGAAAVQTLGARPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8M6</accession>
    <name>U5W8M6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_35530</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561698.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35530"/>
    <dbReference type="EnsemblBacteria" id="AGZ45362">
        <property type="protein sequence ID" value="AGZ45362"/>
        <property type="gene ID" value="AFR_35530"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35530"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7191"/>
    <dbReference type="eggNOG" id="COG3386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024373_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665666" evidence="3">
        <location>
            <begin position="25"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="631"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="704" mass="70092" checksum="ABDABE8354B03919" modified="2014-01-22" version="1">MRRLVFPVILASGLVMLGPVPASAEPAPAAPSAKAAAGKKVCKITSSSLPELSGIVATKNGFAVVNDGGNDDSLSKVFFLDDKCEITKRVDYTGDGPRDTEDMALSPDGKTIWVADIGDNNVRDNPDDPRGTVGLWSMPSDGSKRPKLHRLAYPDGDKHDAEALLLNGDGTPIIVTKETVGKPSGIYVPTGPLKVNNDEGVKLKKAGEVTLPRTNTAGSPLARLAQGVVTGAAVAPGGSKVVLRTYLDAFEWDVADGDVLAALKGKPRQTGLPNEPFGEAITYGTDGKQFFTVSDIASSPDPEADSYILRYTPATKVVEANALAADGKGGADKASWYSDLSLTDITYLVSAVGILGAILVGAGIFGIVRSRKKRPPEPATKSVDPTGPKPLDAETELLSVGGAAALQRPGVYGGNRPPAGAPSGPVYGGKPEVAPKKGSGVYGGAAGRPPQGQPPARPGQGQPAGRPGQPAARPGQPAGRPGQGQPGARPGQGQPARPGQGQPVRPGQGGPPPAGRPGGGGRPGGGAQPAQPGRPGGGAQPAQPGRPGGGAQPAQPGRPGGGGGAAQPGRGGQPGRPGQGGQPGRPGGGGQPGRPGGGGAQQPGRPGGGGAQQPGRPGGGGGGGGRPGGGVYGAPPPPPPSTGRPPGGSQRPSGFAGPQGGGGRQPGRPDARDDGMNNYANLNGAPRREGRFDNPGYGRGPRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8T5</accession>
    <name>U5W8T5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Collagen alpha-5(VI) chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_30725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30725"/>
    <dbReference type="EnsemblBacteria" id="AGZ44405">
        <property type="protein sequence ID" value="AGZ44405"/>
        <property type="gene ID" value="AFR_30725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6221"/>
    <dbReference type="eggNOG" id="ENOG5033311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080429_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0176">Collagen</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="288" mass="30528" checksum="D18CA42C8AC36692" modified="2014-01-22" version="1">MPGRASVDAAGRAEVPGAGGDEPVRGTGQVTPPGRVKAAAAVAVPSVGESLRPAAVEEAETPGGLLGSAAELRTQLREKRKLRVITLVTLSVIVLGLLPLIFGIRSATRDPVFNSLDALSVPTWADKRVEDRSSGSRWCFLDCRFRERTAQSDRPFEETDKVYSEALTTAGWKVRGGECADQPKTSGKYTCWTRDEFTLDLWVRLPECAVDALAAQDPASLPSTGPDGVVETVDPATCKGSTVSIKVQNAITDQRGKPEPAVDPSLIGETPDPVLTNDPLLEPTPSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>U5W903</accession>
    <name>U5W903_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="7">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="7">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="7">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">aspS</name>
        <name evidence="10" type="ORF">AFR_30805</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="7"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560756.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30805"/>
    <dbReference type="EnsemblBacteria" id="AGZ44421">
        <property type="protein sequence ID" value="AGZ44421"/>
        <property type="gene ID" value="AFR_30805"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30805"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6236"/>
    <dbReference type="eggNOG" id="COG0173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014330_3_2_11"/>
    <dbReference type="OrthoDB" id="226836at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="PTHR22594:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="135"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="7">
        <location>
            <begin position="192"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="577"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="529"/>
            <end position="532"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="7">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="7">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ44421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="600" mass="65510" checksum="E28E597066BF642F" modified="2014-01-22" version="1">MIRTHNAGSLRASHAGQTVTLAGWVARRRDHGGVIFVDLRDASGVVQVVFREEDAHALRNEFCVKVTGEVTQRPDGNENPDLPTGAVEVTASELVVLSEAAPLPLPVDDAITASDDLRLKYRYLDLRRSGPSNALKLRSRANQIAREVLHAHDFNEIETPTLTRSTPEGARDFLVPVRLQPGSWYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDIEMSFVTQDDIIALGEEIVVKLWGELAGYQVQTPIPRITWTDAMNRYGSDKPDLRYGVELTELTTYLTGTAFRVFAGAIDAGGYVGAVVMPGGASQTRKELDGWQDWAKARGARGLAYVVLDAETGEARGPVAKNLSAEHLAGLGDVVGAKPGDAIFFAAATERREAQELLGAARIEIAKRSGLIDESTWAFCWVVDAPMFEKTDEGGWTAVHHPFTSPNDEWLDKFESAPDQALAYAYDIVVNGNEIGGGSVRIHRGDVQSRVFDLLGITPEEAQDKFGFLLDAFKYGPPPHAGIALGWDRTCMLLSGNESIREVIAFPKTRGGFDPLTSAPTPITTQQRLEAGIDAKPKAPAATPGTDGVAVPVEPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W965</accession>
    <name>U5W965_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_36405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561873.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36405"/>
    <dbReference type="EnsemblBacteria" id="AGZ45537">
        <property type="protein sequence ID" value="AGZ45537"/>
        <property type="gene ID" value="AFR_36405"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36405"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7367"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_340307_0_0_11"/>
    <dbReference type="OrthoDB" id="610681at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="212"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="835" mass="93234" checksum="CBB5D7E7C77A14CA" modified="2014-01-22" version="1">MGETPGLLDDYLRIARYHVRLALPPTAIRIRSLDARQLLARSGLRESGSYEDLLAAARAGDSRWLKQRRREVKPAVLAGLAQTLAQQDMLSEDRVDSLAIYDLIRVALGANALPSAHQGLHAQLAFAWQGPERARELLRAYRQLSESVRTDLELDLANPFAGSGPVIPWLTAFRKMLPDPPLALEHKEGVPPFDRLTVAAAVPPVEEPERISVIVTSFHPDEGLITAVRSILDQSWRNVEVIIVDDASPPEYDAVLARAVALGSRIRLVKQPVNMGTYEARNAGLDAAEGEFAAFQDSDDWSHPRRLELQVRPLLDNRRLVATTSDGLGVSDDLLITRPGVRRGRFNPSSLLFRRALVMGRIGYFDRVRKAADSEYIGRMRAVYGERAVEHVESEPLALIRLSLGSLSRSEIRPYWMHPARTAYMSAYQHWHSRIAARTAKPHRPRDGSRRPFAAPDHLLRARGEDAGRPEYDVVVVADWRFLQGAQQSGLEEIRALVARGLRVAIMQTESLRPPARRRWPLCAPLQQLVNDGRVDQILPGDENRAELVIVRHAAVLQFPDGDPCLLRARQVMIVADEAPTRSEDLDRRYSPEDCSRAATRLFGPEPVWCPQDPQVRALLRAENVELTPYDLPAVINSERWSSNREGASAGLAVVGTDLSDEGVWPRDVREAVVVYESLGRWDVRLRLPDWPRTGYQLGGPRSHLVFDAADLDLRTFVHQLDFYLHFPSPERIETFSRPALEAAAHGCVVVMPERHAAVYGDAAVYCGPGEVEATLERYQSDRELFAEQSRRARAVVAKAHHPQLFVDRIAGIVTAPHPATPVQRSVQRAAGATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9D1</accession>
    <name>U5W9D1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_31575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31575"/>
    <dbReference type="EnsemblBacteria" id="AGZ44575">
        <property type="protein sequence ID" value="AGZ44575"/>
        <property type="gene ID" value="AFR_31575"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31575"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6391"/>
    <dbReference type="eggNOG" id="COG0759">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1914661_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002696">
        <property type="entry name" value="Membr_insert_effic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33383">
        <property type="entry name" value="PTHR33383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01809">
        <property type="entry name" value="YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01234">
        <property type="entry name" value="Haemolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00278">
        <property type="entry name" value="TIGR00278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="129" mass="14406" checksum="73F1A37D357E70BA" modified="2014-01-22" version="1">MSHAYATMVTMWGSRKHKRKRKWHDGCDCGCDGCDLPFCDLSLLSTTLLAFSRLGRSGRRTDAVVTTAIRGYRKVSPRLPTRCRYTPTCSAYGLEAVQRHGTRKGLWLTAGRLRRCRPGVAYGTADPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9J3</accession>
    <name>U5W9J3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GGDEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37190"/>
    <dbReference type="EnsemblBacteria" id="AGZ45692">
        <property type="protein sequence ID" value="AGZ45692"/>
        <property type="gene ID" value="AFR_37190"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37190"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7524"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_129_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="373"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="505" mass="53779" checksum="4978EEA7DCB67A4C" modified="2014-01-22" version="1">MHRPDARRGLRVDPLLVVLAVLALITAVGFALQRATTVAQVATFWLLMAAVHACFAVTSWRVAARSRPSGRHLWSLFAIAGAAFLVGDAGQLVEVARHPAGPSAVVGTSFQFGALAVGVTVLVTGLLRFPLGTMEDNARSRLRLDVATVLAAATTFGLWLFEVPAGDRGGGWFFGLVTAILVLPGMFLVATFAVVRLILSGRTPFTRLTALTGGTAAVMQVVLQAVPGSIYQDPRFMPWLLCANVLASTLLAVAARSQERGARHARQDVTAGPRRRYSILPYAAMVAVWLLTALLLAVEGLDTRAWVVGLGAVVTTVLVIGRQVSDFRAITELLDERDKLTTELSRLAYHDALTGLANRGRFMQRLHDALAAGPVTVFLVDLDDFKPVNDAFGHATGDQLLIEVGRRLRAEVRDGDTVGRLGGDEFAVLMEGLPVDRRKEVADGLSAALSGTVRIGAVDVGLSASVGSATGRQGLQDPDSLLHEADMAMYAVKTDRRILVAGDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9N6</accession>
    <name>U5W9N6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_32160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561022.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32160"/>
    <dbReference type="EnsemblBacteria" id="AGZ44690">
        <property type="protein sequence ID" value="AGZ44690"/>
        <property type="gene ID" value="AFR_32160"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32160"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6511"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_10_0_11"/>
    <dbReference type="OrthoDB" id="2089619at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041678">
        <property type="entry name" value="TetR_C_16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17920">
        <property type="entry name" value="TetR_C_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="192" mass="20351" checksum="0CA6340FD4F782DF" modified="2014-01-22" version="1">MTKPTRADQRRRTEARILATARRLFAELGYDRTTIRGVAGEAGVDAGLVMHYFGSKDKLFARAAEPPVAAASDAEAGDVTEALLTSLADRLDSEPEASLAVLRSMLTNADAADRYRAAAGPELSRITEGIPAADAELRAGLLSAIVHGVIIERYLLRLGPLADASPEHIVELLRPCFRTLAAAPVGGAPRVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Q5</accession>
    <name>U5W9Q5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562273.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38420"/>
    <dbReference type="EnsemblBacteria" id="AGZ45938">
        <property type="protein sequence ID" value="AGZ45938"/>
        <property type="gene ID" value="AFR_38420"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38420"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7772"/>
    <dbReference type="eggNOG" id="ENOG5032MR7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987857_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13383" checksum="621001B266556B4C" modified="2014-01-22" version="1">MTAAILDRAEFLSPVDDQADLCVTALGRQLTAYVAGAGSVEEFESWFAGRARPDRRVAGRLSAAAEVISVFEAANRTTLAAAWLREMDPSGYVPARVLRLSEGDPTTAKALIETAIAWVQEADLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9T3</accession>
    <name>U5W9T3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561009.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32090"/>
    <dbReference type="EnsemblBacteria" id="AGZ44676">
        <property type="protein sequence ID" value="AGZ44676"/>
        <property type="gene ID" value="AFR_32090"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32090"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6497"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050005_0_0_11"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="442" mass="46397" checksum="987ED47870AE1806" modified="2014-01-22" version="1">MSSVIELGLIPGDAPEPPARPRRLRVPPRTGVVVVAVLCALTLTGSALPRSRAPLPLWSIRLDVSVASSFAVTGDGVYVLTGTSQGRRLTAYDRRTGVSRWSVPVARLASSFGLVRAGVIQLAVEPEPAVEWQQDGSRVSRTTGRRTIGIDAATGRQLWMLPGDLSFTRDGLAVAADWDETGSVLLSLRIVDVRSGATVWTHPARGLSSWGLSDTIAASRVVTAAPDGRVEVRDLADGRLVTSRVLPQLTRTGPDDAPADLGVAGDLVLLKEQEALTAHDATTLRERWRLGTERFQGSYGCAPILCVTSDSGTAGVDPATGRQLWRRPGSGLSIDLGAGVVLDQGNDGSRSALIDTATGRTLVDLGSGGVAWNDGEAVEPLPVLLPTRDPAGRTAAGRLDQRTGRVTWLGTIDPVGSFACRSAGAIMACVTGFELTVIDLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-04-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9Y9</accession>
    <name>U5W9Y9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_32685</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the DedA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561133.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32685"/>
    <dbReference type="EnsemblBacteria" id="AGZ44795">
        <property type="protein sequence ID" value="AGZ44795"/>
        <property type="gene ID" value="AFR_32685"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32685"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6614"/>
    <dbReference type="eggNOG" id="COG0586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_2_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032818">
        <property type="entry name" value="DedA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30353">
        <property type="entry name" value="PTHR30353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="194" mass="20313" checksum="3BB27A0F70F4B8A7" modified="2014-01-22" version="1">MISELGTMGWLALVVAFGAIVPIIPTGAAVSGAAALAFHDHPLLIVLVIAAGAAGAYVGDLVMYAMCRAGGEQLARRLRWLRDEEHLAAVKERLHKSQIPVLLVSRLLPGGRVPVLLAAAFLGLSWRTFVVANAPACALWSVVYAGIGVAGGSIFPEPWEGVVAAIILVLIIGQIVNLISKRRAARQQAEAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAD4</accession>
    <name>U5WAD4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_33240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561243.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33240"/>
    <dbReference type="EnsemblBacteria" id="AGZ44906">
        <property type="protein sequence ID" value="AGZ44906"/>
        <property type="gene ID" value="AFR_33240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6727"/>
    <dbReference type="eggNOG" id="COG3897">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074455_1_0_11"/>
    <dbReference type="OrthoDB" id="2049098at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25378" checksum="7C47A760B5784962" modified="2014-01-22" version="1">MKTYSGAPYAVATHSALQRRLLRNASFPARAPQQDALHMTPVPMVPEVHLFLAEDSVLLWARLEAEAGTPLPAPYWATAWPGGQALARYVLDNPEIVAGRRVLDLASGSGLVAIAAAQAGAAEVTAADTDAYAIAAIQANANANGEQIATVVEDLTGGDGSDFEVILAGDCLYNADVAALMLPFIGRAMNRGATVLLGDPGRGYAPPGLLRTLTTYQEAVLGAQEDGQRNETSVLTPVAVALTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAH2</accession>
    <name>U5WAH2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_33660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33660"/>
    <dbReference type="EnsemblBacteria" id="AGZ44990">
        <property type="protein sequence ID" value="AGZ44990"/>
        <property type="gene ID" value="AFR_33660"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33660"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6813"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="177"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="373" mass="39056" checksum="3FE0769357B01FEA" modified="2014-01-22" version="1">MEAVPQSLPWVAATLTLVGVPAGLAITGGNVQGVMTACLAIALALLMFAVRRWPLTVLVVSVLSVIAFRTSVLIGTGWAWPATAAFVALALAGRIRTAVVAGAITIAYGFVWDWVVEQKSTDWVLGHVGGETLFLAAVIAAAVAYRNTRRWQDEVAARLQQSLHEQELEALRRRAEERVGIARDLHDVVSHTLAVVGVHLNVALDAFDDEPGEARDALRLAQDVRGRAMTDLKSLVDVLREGDRIATPAAGLEGLAGLADQVRAAGVDVTVTELGDRSEVPAPVATAVYRVVQESLTNTVRHAGATRVAVSLHYTAVNVTVEVTDDGGGKTTTTEGHGIMGMRERVAALGGALTAGPGPGGGFTVRALIPIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAJ9</accession>
    <name>U5WAJ9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amine oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_33785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33785"/>
    <dbReference type="EnsemblBacteria" id="AGZ45015">
        <property type="protein sequence ID" value="AGZ45015"/>
        <property type="gene ID" value="AFR_33785"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33785"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6839"/>
    <dbReference type="eggNOG" id="COG2907">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028123_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amino_oxidase" evidence="1">
        <location>
            <begin position="40"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="441" mass="47808" checksum="DA2F82545E6FECFD" modified="2014-01-22" version="1">MFIRFQSVARPVPNRHQSFICTPTSWENSVRSVAVIGAGVSGLTAAYVLRDEWDVTLFEADTRLGGHAHTHDVADAGGALAVDSGFIVHNRRTYPLLLRLFAELGVPTQETEMSMSVTCRGCGLEYAGAKGRQGLFVQPPKPRYLATLAQVPVFHRRARALLAGSGNPTLGEFVADGRYTRHFVRHFVVPLVAAVWSCGPEVVADYPARYLFTFLAHHGMLSVTGSPTWRTVSGGSRTYVDLLAKELTTVRAATPVRSVTRSGTGVTVRDDGDDLHSFDAVVVATHADQALALLTDPSDAERRTLGAFRYSSNTTVLHGDESVLPSRENARASWNYELPGCAPDAGPPRVTYDMNRLQRLDAHGRYLVTLNAGDRVREDTVLARMTYDHPIYTPSSVAAQSGLPGLNDGRTAFAGAYHGWGFHEDGCRSGVAAAQSLGVTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAR3</accession>
    <name>U5WAR3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_39900</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39900"/>
    <dbReference type="EnsemblBacteria" id="AGZ46234">
        <property type="protein sequence ID" value="AGZ46234"/>
        <property type="gene ID" value="AFR_39900"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39900"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8073"/>
    <dbReference type="eggNOG" id="COG1210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="PTHR43197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="4">
        <location>
            <begin position="2"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ46234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="292" mass="31124" checksum="DA6657E39FF7AF73" modified="2014-01-22" version="1">MKAVIPAAGLATRFLPATKAVPKELLPVVDRPVLQYIVEEAAAAGISDVLLVTGRGKTSMVDHFDRRPDVEARLEEKGDLERLAAVRRTSELADIYTCRQGEPLGLGHAVGVAASHIGDNAFAVLLGDEFVEEDKPLLPAMLDLQAQTGGIVLAFMEVKPEETSRYGIASVSPSDLGEDVVEVTGLVEKPSPEESPSNLAVLGRYILPAKIFDAIKDTKPGSGGEIQLTDAMATLLADGTPVHGIIYRGHRYDTGMPLGYLQAVVELAAKREDLGEEFRAWLGEFVAGGARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAW3</accession>
    <name>U5WAW3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Porphobilinogen deaminase</fullName>
            <shortName evidence="6">PBG</shortName>
            <ecNumber evidence="6">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="6">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hemC</name>
        <name evidence="9" type="ORF">AFR_40175</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="6">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="6"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562621.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40175"/>
    <dbReference type="EnsemblBacteria" id="AGZ46289">
        <property type="protein sequence ID" value="AGZ46289"/>
        <property type="gene ID" value="AFR_40175"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40175"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8131"/>
    <dbReference type="eggNOG" id="COG0181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_11"/>
    <dbReference type="OrthoDB" id="1450400at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018160">
        <property type="term" value="P:peptidyl-pyrromethane cofactor linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="SSF54782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobil_deam" evidence="7">
        <location>
            <begin position="5"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobil_deamC" evidence="8">
        <location>
            <begin position="225"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="6">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ46289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="31659" checksum="170C91A2A8CB7020" modified="2014-01-22" version="1">MTTPLRLGTRGSALALAQSQLVADSLTAATGRPVELVRIVTAGDRSAAPVAQLGVGVFVSALRDALIANEIDFAVHSYKDLPTAQHPSLHIAAVPQREDPRDALVARDGLILTDLPPGAVIGTGAVRRIAQLHALGLQLQVTPIRGNIDSRIARVLGPGADLDAVVLARAGVVRIGRAAEITETLDPMLMLPAPAQGALAVECRSDDADLVELLAALDHAPSRAAVTAERAMLATLEAGCSAPVAAHAELAEGEQGDEIYLRGAVISPDGARAVRLSRTGTPADAAEIGKALAADLLDAGADTLMGSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WB48</accession>
    <name>U5WB48_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_34515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34515"/>
    <dbReference type="EnsemblBacteria" id="AGZ45161">
        <property type="protein sequence ID" value="AGZ45161"/>
        <property type="gene ID" value="AFR_34515"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34515"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6982"/>
    <dbReference type="eggNOG" id="ENOG502Z8GQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054022_0_0_11"/>
    <dbReference type="OrthoDB" id="427116at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ45161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="323" mass="34594" checksum="CC24B71FF6038014" modified="2014-01-22" version="1">MVGGSVARVEIGGAGVLRLVEDFERDGSAGDELVALAEDRPELVAPHLLRLVETNMWLPAFVFRAMTEDTAGEVVRRIDEGLLPPGLYRLAAGRTAAAVDAFGRWARESPEWLRHFPDGLGKEGHVGGWELDPAGNRRDLTSRSATALVPVGEGGAPVSGGALDGKCGWCGLPMWRLLEVDRVLLADLFPDSPGPLVISTCARCGGYADIFTEQGRFVEENGEPGFLGEDEDGWELPDEGLLALGEARPDPWATGAGRGWSTLGGLPLWGQEPAYPACPRCSRTMPYVGQVTGRDLDGQWGQGCHYLFHDPGCGLSAVVYQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBK3</accession>
    <name>U5WBK3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chemotaxis protein cheW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562704.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40590"/>
    <dbReference type="EnsemblBacteria" id="AGZ46372">
        <property type="protein sequence ID" value="AGZ46372"/>
        <property type="gene ID" value="AFR_40590"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40590"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8215"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_3_3_11"/>
    <dbReference type="OrthoDB" id="1711729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="145" mass="15721" checksum="EC6339BACE35362D" modified="2014-01-22" version="1">MASRQFATFEVADQLFGVEVHTVQEVLSYNEYTPVPLAPPAVGGLFNLRGQVIAAVDLRVQLGLVRQAMKGPVMNVILRGDGEPVSLLVDRIGEVVDLDDEAFEPPPDTLSGPTRELVVGTFKLDGRLMLALDVNQAVDTYRATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBL2</accession>
    <name>U5WBL2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extracellular serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_35645</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45385">
        <property type="protein sequence ID" value="AGZ45385"/>
        <property type="gene ID" value="AFR_35645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7213"/>
    <dbReference type="eggNOG" id="COG5373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1003372_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666265" evidence="3">
        <location>
            <begin position="39"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="269" mass="25096" checksum="4F4867EC01E40ED3" modified="2014-01-22" version="1">MKTKHLARHRGVRAMARLSVIIGAVGVAALAGASAASASTVEISTRGVVVLAEPAGTEGGVTTKVEQDPPGDPEDPPGDPDNPPGEGGGEEPTDPGGEDPGGEDPGGEDPGGEDPGGEDPGGEDPGGEDPGGEDPGGEDPGGEDPGGEDPGGEDPGGEDPGGEEPGDGDNGPGDPTTPPDSGTGGGNGTDDGGDNNGGGNGDDDGGSGNGGNNGDSNDAGGTGGSDNGGGLPVTGFNVVGLAGVGAAIAAGGVGLVLITRRRRPEGADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBL7</accession>
    <name>U5WBL7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="1 2">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_41545</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="1 2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562895.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41545"/>
    <dbReference type="EnsemblBacteria" id="AGZ46563">
        <property type="protein sequence ID" value="AGZ46563"/>
        <property type="gene ID" value="AFR_41545"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41545"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8406"/>
    <dbReference type="eggNOG" id="COG0634">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073615_0_0_11"/>
    <dbReference type="OrthoDB" id="1819460at2"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="3">
        <location>
            <begin position="24"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="191" mass="21155" checksum="02F09896B1C802ED" modified="2014-01-22" version="1">MADGSWYDADIDRVIISEEQIREKTEELAKQVAADYADAEGGILLVCVLKGAVMFMADFARALGRQGPSSEMEFMAVSSYGSGTTSSGVVRILKDLDRDITGRHVVIVEDIVDSGLTLSWLMKYLQSRQPESVEVVALFRKPDAIKVPVPTKYVGFDIPSEFVVGYGLDFSERYREVPYVGVLKPEVYARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBP2</accession>
    <name>U5WBP2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF402 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_40815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562749.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_40815"/>
    <dbReference type="EnsemblBacteria" id="AGZ46417">
        <property type="protein sequence ID" value="AGZ46417"/>
        <property type="gene ID" value="AFR_40815"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_40815"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8263"/>
    <dbReference type="eggNOG" id="COG3557">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120820_0_0_11"/>
    <dbReference type="OrthoDB" id="1473648at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007295">
        <property type="entry name" value="DUF402"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035930">
        <property type="entry name" value="FomD-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04167">
        <property type="entry name" value="DUF402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159234">
        <property type="entry name" value="SSF159234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF402" evidence="1">
        <location>
            <begin position="56"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="168" mass="19134" checksum="443651E76B1E704B" modified="2014-01-22" version="1">MPSEMVRVVYTKYDGTAHRDYPARRLAEDDLGIWVGVTRGTASVYHGQPSVEQIPFVLLIPHHAWWTGMFNPPPRTSEVYCDITTPARWEGDTVHIIDLDLDVSRRRDTGLIELRDEDEFEEHRVAFGYTAEMVTEAHAAARRLFVALGDGSEPFASAYRKWLSTVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WBY5</accession>
    <name>U5WBY5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PadR-like family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563039.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42270"/>
    <dbReference type="EnsemblBacteria" id="AGZ46708">
        <property type="protein sequence ID" value="AGZ46708"/>
        <property type="gene ID" value="AFR_42270"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42270"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8557"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_4_0_11"/>
    <dbReference type="OrthoDB" id="1706427at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="8"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="105" mass="11415" checksum="99A4FC36C6A795CD" modified="2014-01-22" version="1">MREATYFILAALQDEPRHGYAIIKQASVLSQGRVKPATGTLYTALDRLTEEGFIHVANEEVVNGRARRYYALTPTGRTALQVEAAHLAEAARVVIGRPRPAAGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC78</accession>
    <name>U5WC78_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="14">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Alpha-IPM isomerase</fullName>
            <shortName evidence="14">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">leuC</name>
        <name evidence="17" type="ORF">AFR_36695</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 14">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 14">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis-aconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:17941"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="ChEBI" id="CHEBI:57872"/>
            <dbReference type="EC" id="4.2.1.99"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Organic acid metabolism; propanoate degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="14"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561931.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36695"/>
    <dbReference type="EnsemblBacteria" id="AGZ45595">
        <property type="protein sequence ID" value="AGZ45595"/>
        <property type="gene ID" value="AFR_36695"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36695"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7423"/>
    <dbReference type="eggNOG" id="COG0065">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_11"/>
    <dbReference type="OrthoDB" id="749418at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00946"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047456">
        <property type="term" value="F:2-methylisocitrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 14" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 14" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="12 14 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="16">
        <location>
            <begin position="13"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="434"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="436"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AGZ45595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="481" mass="51298" checksum="99D943C6A7CDD102" modified="2014-01-22" version="1">MVGATPKPRTLAEKVWDDHVVRAAEGEPDLLYIDLHLLHEVTSPQAFDGLRLAGRKVRRTDLTLATEDHNTPTGYADPAFNTRRGDLMTILDTVSRTQIETLRKNCAEFGVQIRSLGNVDQGIVHVIGPQLGLTQPGTTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLPQAKPKTMAVVVSGELQPGVSAKDLILALITQTGTGGGNGHIVEYRGEAIRKLSMEGRMTICNMSIEWGAKAGMIAPDETTFAYLEGREHAPKGADWDAAVAYWKTLPSDEGAEYDTEIHIDASTVSPFVTWGTNPGQGAPLDSAVPDPEEFVEESDRNAARRALEYMGLEAGTPLKDVAVDVVFVGSCTNGRLEDLRAAADVLRGHQVHADVRMMIVPGSYQVREAAEAEGLDKVFTEAGAEWRFAGCSMCLGMNPDTLDPGQRAASTSNRNFEGRQGRGGRTHLVSPQVAAATAVAGKLAAPADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC87</accession>
    <name>U5WC87_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ECF subfamily RNA polymerase sigma-24 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_42920</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42920"/>
    <dbReference type="EnsemblBacteria" id="AGZ46838">
        <property type="protein sequence ID" value="AGZ46838"/>
        <property type="gene ID" value="AFR_42920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8683"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_11"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="39"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="141"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ46838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="243" mass="25456" checksum="175307123E211A3D" modified="2014-01-22" version="1">MTTGDAQTDATGPGESPEPTDAELLRSHVDGDPHAFNELVRRHRDRLWAVALRTIGDREEAADAVQDALLSAHRGAARFRGDSAVTTWMHRIVVNACLDRIRRRQAHPTVPLPDGRHSDDGGVGGTEPTAPAPDHDTALVVRAALAALPAEQRAALVLVDVQGYPVIEAAEILGVAEGTIKSRCARGRARMALALGHLRTGSDEPPGRGDGGHAGNRGAAARVPSGSGAAAQHPSVPDRGDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC97</accession>
    <name>U5WC97_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcriptional regulator WhiB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">whiB</name>
        <name evidence="13" type="ORF">AFR_41665</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a transcriptional regulator. Probably redox-responsive. The apo- but not holo-form probably binds DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="11">The Fe-S cluster can be nitrosylated by nitric oxide (NO).</text>
    </comment>
    <comment type="PTM">
        <text evidence="11">Upon Fe-S cluster removal intramolecular disulfide bonds are formed.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the WhiB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_41665"/>
    <dbReference type="EnsemblBacteria" id="AGZ46587">
        <property type="protein sequence ID" value="AGZ46587"/>
        <property type="gene ID" value="AFR_41665"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_41665"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8432"/>
    <dbReference type="eggNOG" id="ENOG5032TCV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106245_2_4_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035731">
        <property type="term" value="F:dinitrosyl-iron complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01479">
        <property type="entry name" value="WhiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034768">
        <property type="entry name" value="4FE4S_WBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003482">
        <property type="entry name" value="Whib"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38839">
        <property type="entry name" value="PTHR38839"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02467">
        <property type="entry name" value="Whib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51674">
        <property type="entry name" value="4FE4S_WBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="4Fe-4S Wbl-type" evidence="12">
        <location>
            <begin position="10"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ46587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="98" mass="11121" checksum="D343AC33CE844994" modified="2014-01-22" version="1">MISDWPSMAACQSGDPDALFVQGAEQNVAKRICRSCPVRYECLADALDNRIEFGVWGGMTERERRALLRRHPHVASWRKMFEAALREKGADKVLVSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCH9</accession>
    <name>U5WCH9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562040.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37255"/>
    <dbReference type="EnsemblBacteria" id="AGZ45705">
        <property type="protein sequence ID" value="AGZ45705"/>
        <property type="gene ID" value="AFR_37255"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37255"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7537"/>
    <dbReference type="eggNOG" id="ENOG502ZAIR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_534083_0_0_11"/>
    <dbReference type="OrthoDB" id="1863552at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046264">
        <property type="entry name" value="DUF6297"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19814">
        <property type="entry name" value="DUF6297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="502" mass="52651" checksum="85F7FC372352BFCC" modified="2014-01-22" version="1">MTATAHPVSVAELRRWLRRTQSAHRERGETLGNVYFAVLFVLIVGGIVHKQILAVVWPAEANASELAGYSLMLVLTGGLYLTLRRLGPLALSRPAASWLLTAPVSRRRLLLPSLWVALIGAAVAGAAGGLTVLGHVAARPVPGADGLLPLVGSLLGGALLLVALGAQAERRWSSWSDRAASVLVAAGLAGLLADSALGAPRAEPGWPAEPVVTTLAVALLVLVVGLLLAAVRRLAGTPNERILEASKTAGTLFDSAYGVEPSFITEMVERRYWAHRKLRSRRLWHRVPVLVAQDLLMLRRRPGRVLWVAAAATLPVLLGNAPGWVVGLAVLAGGLLPGGVTAGTIRTDAGNPFMLRLLGLSSYEAIKQRLWVPGVLSGLWYAVALGLLQALGDLPAGPWWALGLALGPVGAAATMRRSRVGFVDNGLMPLDTPMGSMSTGPVLNAVAGLDVLLLGLPVIVLIAVGEPLTWTGVLVQIALGVLGARAYLHATTEKDRVELSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCM3</accession>
    <name>U5WCM3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Ribokinase</fullName>
            <shortName evidence="10">RK</shortName>
            <ecNumber evidence="3 10">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rbsK</name>
        <name evidence="13" type="ORF">AFR_37085</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="3 10"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562007.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37085"/>
    <dbReference type="EnsemblBacteria" id="AGZ45671">
        <property type="protein sequence ID" value="AGZ45671"/>
        <property type="gene ID" value="AFR_37085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7503"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_2_0_11"/>
    <dbReference type="OrthoDB" id="1030724at2"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01174">
        <property type="entry name" value="ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01987">
        <property type="entry name" value="Ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011877">
        <property type="entry name" value="D_ribokin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="12">
        <location>
            <begin position="5"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="41"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="212"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="244"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AGZ45671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="301" mass="30485" checksum="DFB702E58D496F33" modified="2014-01-22" version="1">MSSPRIVVAGSANMDLVGLAARLPLPGETVLGDDFVMTPGGKGANQAIAAARAGGACTFLGAIGSDAFGVTIKARLNASEVDTENLRTSYGSSGVAVIMVDRAGENSIMVSPGANSSFAGLSATETAIIAECDVLVCQQEIPVDTVVAAAQAAREGNTRVILNAAPARDLPPELVAAVDLLVVNENEALAITGTPELDMAGLLAVVPRVVLTMGGAGSWYADREGRDERIPACAVDVADTTAAGDAFTGALAVAWGEGRDLVDAVRWANAAGAACVRKVGASNSLPSRAEIDDLYGRTFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCP0</accession>
    <name>U5WCP0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562026.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37185"/>
    <dbReference type="EnsemblBacteria" id="AGZ45691">
        <property type="protein sequence ID" value="AGZ45691"/>
        <property type="gene ID" value="AFR_37185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7523"/>
    <dbReference type="eggNOG" id="COG4213">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_13_3_11"/>
    <dbReference type="OrthoDB" id="859573at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004666516" evidence="1">
        <location>
            <begin position="24"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_4" evidence="2">
        <location>
            <begin position="48"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="370" mass="38916" checksum="81C2EA16B3712895" modified="2014-01-22" version="1">MRTKMFALVAVGLIALGGTAACTDDDGDGGTSGGSGNRGGGGGKVGVILPDTTTSQRWGTDDPKLLKAAFDAADVPVDIQNAQGDKAKFASIADGMINGGVKVLITVNLDSGTGKAVLNKAKAAGIKTIDYDRLTLNGGADYYVSFDNVAVGEQQGKGLTRCLTEKNAKSPLIADLNGSPTDNNATLFKDGYESVLQPKYDDGTFLKGPDQWVADWNNKEAGVVFQEMWNQTGRKIDGVLAANDGLANAAIEVLKKNNMNGKVPVTGQDATVQGLQNILAGDQCMTVYKAIKKEADAAADLAIKLFKGETPTTVDRVKDPESGAYVQSVLEDPQAIFLEDINDVIKDRFVEKKAVCSGRFAALCREAGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5WF76</accession>
    <name>U5WF76_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_42180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563021.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42180"/>
    <dbReference type="EnsemblBacteria" id="AGZ46690">
        <property type="protein sequence ID" value="AGZ46690"/>
        <property type="gene ID" value="AFR_42180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8536"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080128_0_0_11"/>
    <dbReference type="OrthoDB" id="1623593at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="257" mass="27824" checksum="CC2E47ABE5046E65" modified="2014-01-22" version="1">MTAALDRPQATRKQLHWEITLVLLLSLGSSAVYAVVSLIAKLTAGPPLSQQAAVLNRSQSPRVYLDLTYQLLGIFFALVPVLLVLYLLNRDRLDPARTLGIDFRKPGKDLGWGTALAAAIGLPGLLLVYAAAQLGLNAQIVPAALNPVWWAVPVLILSAAQNAILEEVIVVGYLVTRLRELGWRLGYVVAASAVLRGSYHLYQGFGAFVGNAVMGVVFSLFFLKKGRVMPLIVAHTLLDVAAFVGYTLLPPSWLSWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNQ0</accession>
    <name>U5VNQ0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357371.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00690"/>
    <dbReference type="EnsemblBacteria" id="AGZ38427">
        <property type="protein sequence ID" value="AGZ38427"/>
        <property type="gene ID" value="AFR_00690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.139"/>
    <dbReference type="eggNOG" id="COG3255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118604_0_0_11"/>
    <dbReference type="OrthoDB" id="1982614at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039543">
        <property type="entry name" value="POX18/UbiT/NSL-TP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="PTHR10094"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SSF55718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="53"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="150" mass="16228" checksum="4F75151CC93BD639" modified="2014-01-22" version="1">MTDFSPESFATIGPKEFAQLVKSTPDSKLAEVMGNENRSKILDEVFNRMPTLFRADRAGATQAVIHWNITGGPGGSTDTYETVIENGACTVTNQPVREPKLAMTMDPVTFLKVVTGDGNPMMMFMTGKIKAKGDLGLAAQVAKLFDIPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNZ6</accession>
    <name>U5VNZ6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Spore cortex-lytic enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_01165</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01165"/>
    <dbReference type="EnsemblBacteria" id="AGZ38522">
        <property type="protein sequence ID" value="AGZ38522"/>
        <property type="gene ID" value="AFR_01165"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01165"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.232"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069585_2_0_11"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665167" evidence="4">
        <location>
            <begin position="31"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="5">
        <location>
            <begin position="70"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="6">
        <location>
            <begin position="139"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="258" mass="27449" checksum="5C0477129F609CDE" modified="2014-01-22" version="1">MRKALSFAAAIAVTATLAVGCDSGGSDQAAAPPPAPSSAPASPSVQPTTPAAPKPKPKPKPLKLKTGAKGAEVVALQERLNELGYWNGKADGKFGGQTQQAVYALQKAAGLGRDGTVGPKTKKALDQGVRPKAKSTDGYVIEISLKRQLLMLVKDGKVSEVFNTSTGSMENYEQEGNTFLADTPSGKFKVSRQIDGWRHAPLGLLWRPKYFNGGIAVHGANSVPPYPASHGCARLSISAMNWLWKNDKIPIRTRVWVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP63</accession>
    <name>U5VP63_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative MerR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_01425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357517.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01425"/>
    <dbReference type="EnsemblBacteria" id="AGZ38574">
        <property type="protein sequence ID" value="AGZ38574"/>
        <property type="gene ID" value="AFR_01425"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01425"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.287"/>
    <dbReference type="eggNOG" id="COG2452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2393241_0_0_11"/>
    <dbReference type="OrthoDB" id="2076478at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="2">
        <location>
            <begin position="7"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="91" mass="9813" checksum="57FDFFA884235B39" modified="2014-01-22" version="1">MDTGDRLLTPGEVAALFRVDPKTVTRWAAAGRIGSIRTPGGHRRFRESEVRALLEGDGVVEEMREDDAANRPRNGGPTNPGSSYGPPNGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPD3</accession>
    <name>U5VPD3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357597.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38654">
        <property type="protein sequence ID" value="AGZ38654"/>
        <property type="gene ID" value="AFR_01825"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01825"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.369"/>
    <dbReference type="eggNOG" id="ENOG502ZWB4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665681_0_0_11"/>
    <dbReference type="OrthoDB" id="1615840at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019692">
        <property type="entry name" value="DUF2581"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10756">
        <property type="entry name" value="bPH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="159" mass="17927" checksum="161DFE9FB18ADA3F" modified="2014-01-22" version="1">MGDEWTRPYTPGTGRWLVIGWEAIALGLLGWSTVEQFGLAGPGIRVLATAIAALWVIGGWRIREMGVYVGPGGVRIRGLLRSRTMRWEDVAHVRLHRHTRKIGGFQLEGGMTVLIERRDGTMVNTELWAQGIDFHSRPHLFREVYHELRNRHLAAVAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPE8</accession>
    <name>U5VPE8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thymidylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tmk</name>
        <name evidence="7" type="ORF">AFR_01925</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01925"/>
    <dbReference type="EnsemblBacteria" id="AGZ38674">
        <property type="protein sequence ID" value="AGZ38674"/>
        <property type="gene ID" value="AFR_01925"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01925"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.390"/>
    <dbReference type="eggNOG" id="COG0125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_293678_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Thymidylate_kin" evidence="6">
        <location>
            <begin position="453"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="653"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="700"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="765"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="927"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="987"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="455"/>
            <end position="462"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ38674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1034" mass="106527" checksum="6EB2F68DF681AF08" modified="2014-01-22" version="1">MLSVASFGDWIGTLATALFASQQVTGSVAQGSAFSSTIAIRLLPALLLGPIAGVMADRFDRRYTMVICDILRFVIYGSIPVVALFSDSGGLTVTWAVIATFLGETVTLMWIPAKEAAVPNLIPKGRLEVSNQLTLITTYGITPILAALALAALDGVVRGPIGGLPDWAEPVQIALWINAFSRAATAAVVFFGIKEISEGHRERKEKNAETSMFRQFVEGWKYIGGTPFVRGLVLGIFGAFAGAGIVIGTARFFTASLGAGDAAFYLLFATLFIGLAIGIGLGPMIVKELSRRRWFGMSIVLASGAVGFLSVAFHLSMALFGALFVGAGAGMAFLAGVTLLGGQVNDEVRGRVFAVVQIGARLVLLLAISLAGLLVGLGSSRTVSAGSISVDISSTRILLLVAGVIGVWTGISAFRQMDDKQGVPVLADLWSSIRGRPLSPGEQFTRQGIFVVFEGGEGAGKSTQVTRLSEALIEAGHDVVVTREPGATDMGARIRSLVLSNGDNSDAPSPRAEALLYAADRAHHVATVVRPALGRGAVVISDRYVDSSLAYQGAGRTLPVQEISWLSSWATGGLKPDLVVLLDVDPTVGLSRVDSRGQGTDRLESESRSFHERVRYAFLDLASADPKRYLVLDAARPAEEIADVVATRLNSLLTSGDDSHPEPASTAVPPGPDLNAPDLAASHAGPDETLIMQTGASPDATMVIRPGAGPDDTMLVRPADSDDPNATMVVRAASTDPNATMVVRPASTDPNATMVVRAPSSDDPDATSVVRSPSTDPDATSIVRSPSTDPDATSIVRSPSTDPDATSIVRLPSTDPDATSIVRSPSTDPDATSVVRSPSTDPDATSVVRSPSTDPDATSVVRAPADDADATSVVGPASDDPEATTVVGASADDLEASSVVSSAPVEQPAVAEPGSDDADAGSVGSPAPESEETTSADAVTTSADADADGVSAAPASSGDGELGAVDGEVSERAGSASGGEGGARGAERVGAGERVERVGAVDEDETPYEILEDLTGNRAQAAGDDEPPQVGTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPP9</accession>
    <name>U5VPP9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_03245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357881.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03245"/>
    <dbReference type="EnsemblBacteria" id="AGZ38938">
        <property type="protein sequence ID" value="AGZ38938"/>
        <property type="gene ID" value="AFR_03245"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03245"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.653"/>
    <dbReference type="eggNOG" id="COG3254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100689_3_0_11"/>
    <dbReference type="OrthoDB" id="1694303at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016857">
        <property type="term" value="F:racemase and epimerase activity, acting on carbohydrates and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008000">
        <property type="entry name" value="Rham/fucose_mutarotase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05336">
        <property type="entry name" value="rhaM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="110" mass="12670" checksum="0EC8AB53E176DF63" modified="2014-01-22" version="1">MRYCFHLQVREDRIAEYVERHQAVWPEMQSALRATGWRNYSLFLRPDGLLIGYVEADNLDASRAAMDATDVNARWQAEMAPFFAGTTPEEGFPLLTEVFHLSEDEDTEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQL6</accession>
    <name>U5VQL6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">AFR_03940</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358022.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03940"/>
    <dbReference type="EnsemblBacteria" id="AGZ39077">
        <property type="protein sequence ID" value="AGZ39077"/>
        <property type="gene ID" value="AFR_03940"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03940"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.796"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_11"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="212"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="289"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="514" mass="54287" checksum="1ADCC024AAEC02B4" modified="2014-01-22" version="1">MSSSPPTDLAASRPARWNPAGWPLRTRLVAIMVGLLVLLGLVVGAAAEIYLHRTLYQQVDAKLIETNGRNRPPPLNGGGGYYRGDRPPPGSETGWIVLRLDTAGQVLGDGGIVRLSQSSEGYTDLGFSDLPGSTVAQLAAEVPDNRPRDLDLGGDLGDYRIVSSINATGGVSVTAIPLASTQATLLQVALVTGGAVLAALLIAGWAGAVIIRRTLQPLSRVAATATKVSELELDRGKVDLAQRVPAADTDPRTEVGAVGAALNRMLDHVGNALEARHASETQVRQFVADASHELRTPLAAIRGYAELSRRSRQPVPEEIGHVLRRVESEATRMTALVEDLLLLARLDAGRPLAHDPVDLTMLVVDAVSDAHAAGPRHDWQLDLPEEPVTITGDGQRLHQVLANLLANARTHTPEGTTVTVGVGQDGNTALLSVTDEGPGIPEELQPHIFERFARGDSSRSRAAGSTGLGLSIVHAVVTAHGGTVSMHSAPGRTEFIVRLPMTASVAKQPEFVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VR13</accession>
    <name>U5VR13_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 7">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsI</name>
        <name evidence="9" type="ORF">AFR_04790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04790"/>
    <dbReference type="EnsemblBacteria" id="AGZ39247">
        <property type="protein sequence ID" value="AGZ39247"/>
        <property type="gene ID" value="AFR_04790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.967"/>
    <dbReference type="eggNOG" id="COG0103">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_B">
        <property type="entry name" value="Ribosomal_S9_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_S9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ39247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="189" mass="20308" checksum="B29D78E4865B1D80" modified="2014-01-22" version="1">MTDIVEPEVVETVEEPAAEAPAETPAEAPAETPAEASAETPAETVVVVEAPAPAVRAPRPGDRPIQTVGRRKEAIVRVRLVPGTGKITCNGRDLEAYFPSKVHQQLIREPLATAEKPEMFDVVANLRGGGITGQAGALRLGISRALITNDPDDRPALKKAGFLTRDSRVKESKKYGLKKARKAPQYSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRH4</accession>
    <name>U5VRH4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_05875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358995.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ39464">
        <property type="protein sequence ID" value="AGZ39464"/>
        <property type="gene ID" value="AFR_05875"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05875"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1193"/>
    <dbReference type="HOGENOM" id="CLU_1238035_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665995" evidence="2">
        <location>
            <begin position="23"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ39464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="23356" checksum="9EC238D7A44940F1" modified="2014-01-22" version="1">MTVSKPVSAAAVVVAIVLGACAAGGGDEPPPPPAPRPKKQTVSKPAQRSAALVLTQNGLPGLELNRQVLDVSTSDMEAAFDNHDFLNAGQTNTCGLAANEDLGVAAITNTDLAVVAFVVERPDTATTEGIRLGSTTDDIIKAYGKNAVYARDVPSRAGGQLIWVTDLDSPDSEPSASSLHYAFETDTTGTVTRLRAGFWPHVAHIDFCSDKATRTKETGWPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRM3</accession>
    <name>U5VRM3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_06520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359124.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_06520"/>
    <dbReference type="EnsemblBacteria" id="AGZ39593">
        <property type="protein sequence ID" value="AGZ39593"/>
        <property type="gene ID" value="AFR_06520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_06520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1322"/>
    <dbReference type="eggNOG" id="COG4643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_520362_0_0_11"/>
    <dbReference type="OrthoDB" id="248405at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="523" mass="57205" checksum="65B4904921F2D40D" modified="2014-01-22" version="1">MTDDRPRIKVGTGPETIRALKTALRSRVLPDTYVAGGALVYTEQVSGDLASVAADEDSPLPIASSPLNPASLAGLLADHAYVYRMRTRKDANGDPELVEEEFTPPRDALASALVAKTWPEVPPLRGIIGAPVLRRDGTLLQKPGYDPATGLYLASKVALAPVPDKPTPVQVAAARTFLLDRFLRDFPWAGPADRANYLALLATPVLRRFVRSLTPFALIDATMPASGKTILTAGPGMLYGQRVLPWADTEEELRKSITSVFAQQVGVVIWDNLAEGTVIDSANLALLVTGGVWSDRSLGTNNTMAAVNDRLWMATGNNLQVGGDMASRTVRVHLDPNMPRPEARDQSGFGIPHLDQWITVPANQLTVLWHLLILILDWTGNDAPKVTSLSMRQFTPWAQALGGFLTHHGITGFLTNLADVRDIDEDESRWQAFLAAWHERHGNTPMTAAELRADAEPDLLNGMHIDRWDGQFITTTTGRLPNPLQLGRLLTGQNGRWRGEYVIRAGKHERGNRAVFHVEQHEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRM4</accession>
    <name>U5VRM4_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA_edit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00085"/>
    <dbReference type="EnsemblBacteria" id="AGZ38306">
        <property type="protein sequence ID" value="AGZ38306"/>
        <property type="gene ID" value="AFR_00085"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00085"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.18"/>
    <dbReference type="eggNOG" id="COG2606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104555_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="SSF55826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_edit" evidence="1">
        <location>
            <begin position="49"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="183" mass="18760" checksum="6B0F3BED7494FC67" modified="2014-01-22" version="1">MIGTLKLEPAISRLDLLGAPVAAALEVWPAEAPIDGDQILVAPIDAGLADTAAFCDAYGVSLAESANCVIVAGRRGEVTRYAACVVLATTRADVNGVVRKLLDARKASFAPMDDAVQLTGMEYGGITPIGLPKSWPVLVDSRVVAMPHVIIGSGVRHSKIAIAGPALGALPGAEVIEGLAREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRV1</accession>
    <name>U5VRV1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Redox-sensitive transcriptional activator soxR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_00535</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357340.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00535"/>
    <dbReference type="EnsemblBacteria" id="AGZ38396">
        <property type="protein sequence ID" value="AGZ38396"/>
        <property type="gene ID" value="AFR_00535"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00535"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.109"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_7_1_11"/>
    <dbReference type="OrthoDB" id="1661714at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="146" mass="16375" checksum="0B1E889D985F3E4F" modified="2014-01-22" version="1">MYEEISISVEQASDAKVLIISVAARLAGMHPQTLRQYDRLGLVQPGRAGGGGRRYSERDVALLREVQKLSQEEGVNLAGIKRIIGLEQLASELQERVAELERQLGDAYQRIAQLETMHAYPGRDLVRQERPSTALVVWRPRRAGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS72</accession>
    <name>U5VS72_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07075"/>
    <dbReference type="EnsemblBacteria" id="AGZ39704">
        <property type="protein sequence ID" value="AGZ39704"/>
        <property type="gene ID" value="AFR_07075"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07075"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1444"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="230" mass="23045" checksum="09E70671BA261C68" modified="2014-01-22" version="1">MLLMERSVLVTGGTGGLGGAVLETLQDQGWRVVAPVRPGKTGRLPGGIVEVEADLDDAADVQAAVDVAAAETGAPLRAVINVAGGYAGGGLVHETPIEDFEAILRANLRPTYLVTAAALPRLVAAGGGSVVCVASRAAVAPFSGAAGYATAKAAVLAFANAVAVEYKKSGVRCNTVLPSVIDTPANRASQPDADFARWVTPAEIAQVIAFLAGDASAPTSGASIPVYGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSA4</accession>
    <name>U5VSA4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ECF subfamily RNA polymerase sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_07250</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359270.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07250"/>
    <dbReference type="EnsemblBacteria" id="AGZ39739">
        <property type="protein sequence ID" value="AGZ39739"/>
        <property type="gene ID" value="AFR_07250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1480"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_22_0_11"/>
    <dbReference type="OrthoDB" id="1335404at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014303">
        <property type="entry name" value="RNA_pol_sigma-70_ECF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02957">
        <property type="entry name" value="SigX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="8"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="7">
        <location>
            <begin position="103"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="289" mass="31507" checksum="3BEBABD58BA9C81F" modified="2014-01-22" version="1">MTDDPFVTHRSLLFTVAYEMLGSAADAEDVVQETWLRWADVARAEVREPRAYLVRIVTRQALNRLRTVSRQREDYVGEWLPEPLLTSPDVAEDVELAESVSVAMLTVLETLGPTERAVFVLREVFETPYDEIAEAVGKSPAAVRQIGHRAREHVAARRPRVRVSPAEQAAVVERFLAAVRGGDVQGLLDVLAPDVVLVADGGGVVGAARKPIVGADKVAPLLGKLAQLDFDFDVLPTWLNGAPAGRLDLDGALDTAVSVVIEDGRISRIFAVRNPHKLGRLGEEAEISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSH2</accession>
    <name>U5VSH2_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="8">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">ileS</name>
        <name evidence="11" type="ORF">AFR_07625</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359345.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07625"/>
    <dbReference type="EnsemblBacteria" id="AGZ39814">
        <property type="protein sequence ID" value="AGZ39814"/>
        <property type="gene ID" value="AFR_07625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1555"/>
    <dbReference type="eggNOG" id="COG0060">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_11"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="PTHR42780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="9">
        <location>
            <begin position="25"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="10">
        <location>
            <begin position="694"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="609"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AGZ39814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="1038" mass="115304" checksum="E25E5128CC8145A6" modified="2014-01-22" version="1">MAYPKHTEATGVPASPDLPAVERAVLEHWTADKTFEASVDQRPAGQDGSNEYVFYDGPPFANGLPHYGHLFTGYVKDLVPRYQTMRGKHVERRFGWDTHGLPAEVEAEKQLGITTKAQILELGVDKFNEACRTSVLAYTKDWERYVTRQARWVDFGNDYKTLDPDYMESVLWAFKTLHEKGLVYEGFRVLAYCFRCETPLSNTETRMDDVYRDRTDPALTVTFQLETGEKIAVWTTTPWTLPSNLALAVGPDIEYAILEKDGEKLIVGASRVAAYAKDLDGYEQVGTVLGRELAGRRYTPMFDFLVEQAGPNAFQVLAADYVSDEDGTGVVHQAPAFGEDDQNVCNAAGIPTIVTVDEHTRFTALVPAYQGLQVFDANKPIIAELKGRGVVLRHEGYTHSYPHCWRCDTPLVYKAVSSWFVAVTQFRDRMVELNQEITWTPAHIKDGSFGKWLANARDWSISRNRFWGSPIPVWKSDDPTYPRVDVYGSLAELERDFGVAVTDLHRPYVDDLTRPNPDDPTGQSTMRRVPEVLDCWFESGSMPFAQVHYPFENKDWFEHHYPGDFIVEYIGQTRGWFYTMHVLATALFDRPAFRNCLSHGILQGADGRKMSKSLRNYPDVYEVFDQYGSDAMRWMLMSSPVLRGGDMPVTETAIRDSVRQVLLPLWNVWYFFSLYANAAGHDAKLRTDSTHLLDRYILAKTSELVADTTRQLDEYDISGAAGSVRAYLDALTNWYVRRSRDRFWAGDADAFDTLATVLETLCRVVAPLAPLTAEEIWRGLTGERSVHLTDWPEVTAFPADPELVASMDAIREVASAALSLRKAKALRVRLPLAKLTVASPAAEALRPFADLLADEVNVKAVELSADLTAYSQQVLTVVPRALGPRLGKQVQVVIKAVKAGDWELVDGLPVAAGVTLQEGEYELKLVAADAENSAPLPAGRGVVLLDTEVTPELAAEGLARDVIRVVQQARRDADLDVSDRISLLVAAAPGVSAAVETHRDFVAAETLATSLSLDAAATGFSGEVGDGETVTVAVTRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSZ1</accession>
    <name>U5VSZ1_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357709.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02385"/>
    <dbReference type="EnsemblBacteria" id="AGZ38766">
        <property type="protein sequence ID" value="AGZ38766"/>
        <property type="gene ID" value="AFR_02385"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02385"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.482"/>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_6_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="40"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="136" mass="14680" checksum="16A6FD419D4DDE4E" modified="2014-01-22" version="1">MVTTTLLPTAPEGSQGIDQLLSAARARLTRVGPHETYRALARGAVLVDIRPAAQRAEHGEIPGAIVIERNVLEWRLDPRSDARLSFTRYDLEVIVTCQEGYTSSLAAAALQDLGLLRATDLDGGFKAWQEAGLPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTC8</accession>
    <name>U5VTC8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribonuclease II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_09025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359636.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09025"/>
    <dbReference type="EnsemblBacteria" id="AGZ40094">
        <property type="protein sequence ID" value="AGZ40094"/>
        <property type="gene ID" value="AFR_09025"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09025"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1836"/>
    <dbReference type="eggNOG" id="COG0557">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038135_1_0_11"/>
    <dbReference type="OrthoDB" id="1602988at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040596">
        <property type="entry name" value="RNase_II_C_S1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18614">
        <property type="entry name" value="RNase_II_C_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNB" evidence="2">
        <location>
            <begin position="44"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="482" mass="51253" checksum="C2C4FE2858B3708C" modified="2014-01-22" version="1">MPIKRVWAPRIDFSVLRRELELPADFPAEALQEAQEAASIALPPVDRTDIPFVTVDPATSQDLDQAMELTRLPSGGYRVRYAIADVASYVRPGGPLEAETWVRGQTIYFPDGKVPLHPQVLSEGAVSLLPDVDRAAILWTIDLDAEGSFAGIALERARVRSRAKLDYAGLQASVDAGTAPEPIALLPELGALLAARAADRGAVNLPLPEQEVEPDGDGWRLVLRAPHAVEEHNAHISLLTGMAAATLMLDGGVGLLRTMPAARPEAIAKLRAAGESLGVPWPEGSSVGAVVASVDPASPRGAAFLNQVADLLRGAAYTAFDGERPTEIGHGGVGAPYAHVTAPLRRLADRYATEVCLALHEDRPVPEWAREALPRLPKCMAATDRVASAADRGAVSLAEAVVLAHRVGETFEAGVVDVDAPSKPAQPETQKQNNRRPRGGTVALDDPAVQARCLGELPLGDRIQVRLTEADPVKRSVLFEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTL2</accession>
    <name>U5VTL2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_03560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03560"/>
    <dbReference type="EnsemblBacteria" id="AGZ39001">
        <property type="protein sequence ID" value="AGZ39001"/>
        <property type="gene ID" value="AFR_03560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.716"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_17_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="3">
        <location>
            <begin position="138"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ39001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="378" mass="40332" checksum="7901DDA70860DCC2" modified="2014-01-22" version="1">MAVAGFGWMGRVHTQSYLRVLHHYPQLGVRPELVAVADEVPGRAAEAADQYGFTTATLDWRDLAADPRIRAVSIAAPNFLHRELGVALARAGKHIWIEKPVGLDADDARAVAAAAREAGVQGAVGFNYRNAPAVEAARELITAGELGTVTHARFRLFSDYAAHPEGALTWRFERARGGSGVLGDLAAHGIDLVRHLLGDILSLVADTATFVPQRSRPSGATAGHTRAAGGELGPVENEDYVSSQLRLASGARVVLEASRVAVGDQNNYGFEIHGTRGAVSWDFRRMGELAVSVGDDYQDQPRRTVHVGPAAGDYAAFQPGSGIAMGYDDLKVIEAYRFLRSIIETKPYGATLDDAVHSAVALDAMSRSVESGAWVSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTN8</accession>
    <name>U5VTN8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rv2175c_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_09700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359902.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09700"/>
    <dbReference type="EnsemblBacteria" id="AGZ40229">
        <property type="protein sequence ID" value="AGZ40229"/>
        <property type="gene ID" value="AFR_09700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1977"/>
    <dbReference type="eggNOG" id="ENOG5032W34">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134416_1_0_11"/>
    <dbReference type="OrthoDB" id="1752144at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041098">
        <property type="entry name" value="Rv2175c_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18367">
        <property type="entry name" value="Rv2175c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rv2175c_C" evidence="1">
        <location>
            <begin position="70"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="125" mass="13361" checksum="01DB9EC830AE88B5" modified="2014-01-22" version="1">MTDSVTVGEAAAPQPTGPTEWINLPDVADKLGVSISKVHQMIRDGALLATRRDGVRVVPAELVANSTVLKHLPGVLTLLRDNGYNDEEALGWLYEPDETVDGCAAKGLGGDRAREVKRRAQALGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VTW0</accession>
    <name>U5VTW0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinate dehydrogenase/fumarate reductase iron-sulfur subunit</fullName>
            <ecNumber evidence="9">1.3.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">AFR_09790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.1" evidence="9"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359939.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_09790"/>
    <dbReference type="EnsemblBacteria" id="AGZ40247">
        <property type="protein sequence ID" value="AGZ40247"/>
        <property type="gene ID" value="AFR_09790"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_09790"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1996"/>
    <dbReference type="eggNOG" id="COG0479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_3_3_11"/>
    <dbReference type="OrthoDB" id="1605514at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="7">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="150"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AGZ40247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="26366" checksum="A5DDBB7E9AE79BFF" modified="2014-01-22" version="1">MDIQVRVWRQQGPADKGRMVTYDVKDISPDASFLEMLDVLNEKLILDGDDPVAFDHDCREGICGMCGMMINGVAHGPERATTTCQLHMRHFKDGDTIDVEPWRAAAFPVIKDLVVDRGAFDTIIQAGGYITAPTGTAPDAHATPVPKPDADAAFEAATCIGCGACVAACPNGSSMLFTAAKVAHLGLLPQGQPERSSRVVDMLEAQDDAGFGGCTNMGECTTVCPKGIPLTLIGRLNNDYRKSIGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU38</accession>
    <name>U5VU38_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_04680</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358230.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_04680"/>
    <dbReference type="EnsemblBacteria" id="AGZ39225">
        <property type="protein sequence ID" value="AGZ39225"/>
        <property type="gene ID" value="AFR_04680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_04680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.945"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_11"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="323"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="361"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="551" mass="60018" checksum="6D4119DAE73267AD" modified="2014-01-22" version="1">MGYVDVSGAGFVLPDGRELFADVSFRVGEGAKVALVGPNGAGKTTLLRMVAGDTPTPIGTIARAGGLGVMRQFIGMIGDDRTLDDLALSLVAPPLRAAGALLASAQATLDAKPDAEKAQLAYANAIAGWGEAGGYDAEVLFDVVAVGILGKPWEEVRHRPVRTLSGGQQKRFALDLLLRGSDEVLLLDEPDNFLDVPGKRWLETRLRESSKSVLFVSHDRELLAQTADRVVAVEGGSAWTHPGGFASWHEARSDRHERLLELRRRWDEEHEKIKELVFTLKNKAAYNDGLASRYQAAQTRLRKFEEAGPPPLPPKEQSLRMNLRGGRTGKRAVMCTGLELENLTFPFDLEIWYGDRVAVLGANGTGKSHFLRLLAAGGTSPDVDNKPVDGAPLAHVAHNGVARLGARVRPGHFSQTHDRPELLKRTLVEILWRGDDHRSGMDRAGAMKALNRYELAAQGDQVFGTLSGGQQARFLVLLLELSGATVLLLDEPTDNLDLASAEALEEGLQAFEGTVIAVTHDRWFTRGFDRFVLFQSDGEVVETPEPVWDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VU79</accession>
    <name>U5VU79_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoacyl-ACP reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_10700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360298.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_10700"/>
    <dbReference type="EnsemblBacteria" id="AGZ40429">
        <property type="protein sequence ID" value="AGZ40429"/>
        <property type="gene ID" value="AFR_10700"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_10700"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2181"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_11"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="245" mass="24609" checksum="6550AD034924C332" modified="2014-01-22" version="1">MSEFDGLTAIVTGGASGIGAATVALLRERGARVAGLDLTEHDSDLSVICDVGDSASVDAAVATVADSFGRLDILINNAGIGAVGDVTANSDDEWLNVLNVNVVGIARLSRAAMPLLLASPNAAIVNTCSITGWLGLPQRAVYSASKGAVAALTLAMAADHVREGVRVNAVMPGTADTPWVARLLDAADDPVAAGAALRARQPTGRLVTPQEIAHAIAYLASPLASATTGTLLAVDGGLAGVRLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUK7</accession>
    <name>U5VUK7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polysacc_synt_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_05185</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05185"/>
    <dbReference type="EnsemblBacteria" id="AGZ39326">
        <property type="protein sequence ID" value="AGZ39326"/>
        <property type="gene ID" value="AFR_05185"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05185"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1049"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_4_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="366"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ39326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="521" mass="54141" checksum="2637B90DDF93B6B8" modified="2014-01-22" version="1">MTTTAVADPPRRKGTQGVARGGLANMAGSALAGGTGVAVTWVVARGLGAEQAGAFFAATAAFVLAGGLAKLGTQTGLVYWPARLRATGRDHLLGTCLRTGLVPVLTLAVVLGVAMWFGAPAVAHLTAADSPHVVDAHTTGLRVLAVFLPLQALTDALLTATRGYKAMRPTVTLDRILRSTLQLLCVGAVGVAALWTTATLPAFALAWALPYLPVTVLAAFALRRTYLAGRPERPKIRRSEQRQLRRDFWRFTGPRALASVAQLAVQRVDVLLVAALGGLAPAAIYAVAGRFVVLIQFANQGISQSVQPRLAEALATGDRETANHLYQTATGWLVLVTWPINFLVILFAPLYLGLFGDAYRQGADVVIVLACAMLVATGCGMVDMVLAMAGRTSWNLVNVLIALGVTIGLDVLLIPRYGALGAAIGLACAMVANNLLPLVQVGRSARLHPFGAGTLAAGGLSVACFGVLPRVVTAVAGTGAAGLTLAVALAVPAFAAGAWLLRGRLALDAFQPRKLIQRRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUQ5</accession>
    <name>U5VUQ5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease Z</fullName>
            <shortName evidence="8">RNase Z</shortName>
            <ecNumber evidence="8">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnz</name>
        <name evidence="10" type="ORF">AFR_05460</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358912.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05460"/>
    <dbReference type="EnsemblBacteria" id="AGZ39381">
        <property type="protein sequence ID" value="AGZ39381"/>
        <property type="gene ID" value="AFR_05460"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05460"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1107"/>
    <dbReference type="eggNOG" id="COG1234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031317_2_1_11"/>
    <dbReference type="OrthoDB" id="1712770at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="9">
        <location>
            <begin position="32"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AGZ39381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="309" mass="33811" checksum="A3EB225F83AA69A2" modified="2014-01-22" version="1">MRELTVLGTASQVPTRHRNHNGYLLRWDDEVILFDPGEGTQRQMLLAGQAVTPLRRVCITHFHGDHSLGLPGIIQRISLDKVPHPVAMHFPAGGREFFDRLRHATSFYDVADLSPEPVSDGFRVETSAGPLTALPLRHSIETYGYRLAEPDGRRMVPSLLAAHGITGPMVGELQRTGSAGRVTLEQVSAPRPGQRFAFVMDTGLCENVFRLAEGADLLVIESTFLAEDAAMAAQVGHLTAGQAASVARQSGVRTLLLTHFSQRYADSTRFLDEARAEFDGDIVLAEDLMRVPVPPRLGSTDDRASASRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5VUV6</accession>
    <name>U5VUV6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_05735</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05735"/>
    <dbReference type="EnsemblBacteria" id="AGZ39436">
        <property type="protein sequence ID" value="AGZ39436"/>
        <property type="gene ID" value="AFR_05735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1163"/>
    <dbReference type="eggNOG" id="COG1482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026967_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016305">
        <property type="entry name" value="Mannose-6-P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309">
        <property type="entry name" value="PTHR10309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001480">
        <property type="entry name" value="Mannose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00714">
        <property type="entry name" value="MAN6PISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="396" mass="40946" checksum="50A4C65811CD05FF" modified="2014-01-22" version="1">MAAVFPLQGVIRPYAWGSRTAIAELQGRPAPSDAPEAELWLGAHPGDPSTVTGPDGPVSLAALIADDPKGQLGAEVADEFGVRLPYLMKLLAAEAPLSLQAHPDADYAKQAWAAQEADPAAPRNYTDAHHKPEMLVALTAFDALCGFRAPDVSAAAIEGLAIPRLAPVVAALRTGPSGLRDAVQTLLTWPADDRAALITEVVAAAANGPSDHAESYSLATDLAGHYPGDPGVLVALLLNLVHLAPGEAIWMPAGNLHAYLSGTGVEIMAASDNVLRGGLTPKRVDVGELLRVLRFEVLENPILAATEVAPGVQTWQVPVREFALYRLTLTPTTPPLSLPGTGPRIIFGTQGDVYVAEAVDGTPVEVTPGTAAYVPAETGPATVAGVGQIFVASVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV18</accession>
    <name>U5VV18_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peptidase C14, caspase catalytic subunit p20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_12115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_12115"/>
    <dbReference type="EnsemblBacteria" id="AGZ40709">
        <property type="protein sequence ID" value="AGZ40709"/>
        <property type="gene ID" value="AFR_12115"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_12115"/>
    <dbReference type="eggNOG" id="COG1413">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1640164_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ40709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="161" mass="17333" checksum="97FA3DB49718DEE3" modified="2014-01-22" version="1">MKIASSAWTDAGFDMLTIGIHDSDPQVRQAAAESLRGYDEDRARELLVTASNDSVAQVRKGAASALRQPDDRSVATTLLRLAEDRDPSVRGIAVESLGSVGGLEALQQLVRALRVPDSFERIRAAAGLGELLDDRAVEPLAVALKDPQPAVAVRRRAHWVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5VV78</accession>
    <name>U5VV78_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_13135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360972.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_13135"/>
    <dbReference type="EnsemblBacteria" id="AGZ40913">
        <property type="protein sequence ID" value="AGZ40913"/>
        <property type="gene ID" value="AFR_13135"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_13135"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2667"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_4_0_11"/>
    <dbReference type="OrthoDB" id="1748559at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00552">
        <property type="entry name" value="HTH_MERR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ40913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="122" mass="13437" checksum="6C81A86FE759F7F8" modified="2014-01-22" version="1">MRIGDLAARTGVSTRSLRYYEEQGLLGSRRSAGGHRHYTEDEVDRVAYLQRLYAAGLSSETILSVLPCLVSPSQAASDHAFERLVEERDKLVTHIGGLTTTLESLDELIAANRARRAATPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>U5VWI5</accession>
    <name>U5VWI5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chemotaxis protein CheY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_15430</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361427.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15430"/>
    <dbReference type="EnsemblBacteria" id="AGZ41368">
        <property type="protein sequence ID" value="AGZ41368"/>
        <property type="gene ID" value="AFR_15430"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15430"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3133"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_11"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="25387" checksum="2D64E1BD4E7A99A6" modified="2014-01-22" version="1">MRVLVVEDEKRLGWSLRVGLEAEGFAVDLAENGIDGLWLARENEYDVIVLDLMLPGLNGYRICSTLREEKDWTPIVMLTAKDGEWDQVEGLDTGADDYLTKPFSFPVLVARLRAVSRRGARERPTQLEAGDIRIDPAARRVRRGDTELDLTAREFSLLLYLARNTGDVVSKRQILDAVWDADFDGDPNIVEVYIRHLRNKVDRPFGREAIQTLRGAGYRLASDGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VX06</accession>
    <name>U5VX06_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_15610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361463.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15610"/>
    <dbReference type="EnsemblBacteria" id="AGZ41404">
        <property type="protein sequence ID" value="AGZ41404"/>
        <property type="gene ID" value="AFR_15610"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15610"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3170"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_2_2_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041347">
        <property type="entry name" value="MftR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17754">
        <property type="entry name" value="TetR_C_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="15"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ41404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="206" mass="22400" checksum="637CC6F6093F83CF" modified="2014-01-22" version="1">MRSGTDSSIAGRKRQLVADELRDAALMLMATKGFDEVTVDNIVAAAGLSRRTFFRYFASKEDVVVQFLAGLGAGVVAELESRPAAEPPSVALRHAVWVPLAACTDEADHTERARIVVRLVLDHPPLHARWMERQIEWRTGLAAGLAARHGLDPVTDPYPVMAAGMALLALETVVGQWREGDDERRLAELTDRAFVILAPALDGPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXC0</accession>
    <name>U5VXC0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ANTAR domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_16625</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361680.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16625"/>
    <dbReference type="EnsemblBacteria" id="AGZ41603">
        <property type="protein sequence ID" value="AGZ41603"/>
        <property type="gene ID" value="AFR_16625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3371"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074354_0_0_11"/>
    <dbReference type="OrthoDB" id="1958442at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012074">
        <property type="entry name" value="GAF_ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036625">
        <property type="entry name" value="GAF_ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ANTAR" evidence="3">
        <location>
            <begin position="167"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="244" mass="25458" checksum="28D10FDA214AAC0C" modified="2014-01-22" version="1">MAQDPTDPGSVFAELGRIKLSDTDLDGVLDRVAGLAKAKLPGAAEVSITLVPRTGPHTAAFTGDTALQLDEVQYRKGTGPCLDAAAGQATVSVPDTTDDDRWDGWAAHASAAGIGSSLSVGLPILDTIDGAVNIYGRRRHAFDDATIRLARTFAGYAGTALANAHLYDSTASLAQHMQAAMENRAVIEQAKGIIMGERRCTADEAFAILTRASQDSNRKVREVAAALVAATQSPNYQPPGGASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXE5</accession>
    <name>U5VXE5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA ribosyltransferase-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_16775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16775"/>
    <dbReference type="EnsemblBacteria" id="AGZ41633">
        <property type="protein sequence ID" value="AGZ41633"/>
        <property type="gene ID" value="AFR_16775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3402"/>
    <dbReference type="eggNOG" id="COG0809">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039110_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003699">
        <property type="entry name" value="QueA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042118">
        <property type="entry name" value="QueA_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042119">
        <property type="entry name" value="QueA_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036100">
        <property type="entry name" value="QueA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30307">
        <property type="entry name" value="PTHR30307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02547">
        <property type="entry name" value="Queuosine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111337">
        <property type="entry name" value="SSF111337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ41633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="331" mass="35849" checksum="E945A96BB8C30272" modified="2014-01-22" version="1">MIDFALPPALEADRPPPARDGVRMLVGERSSGRVTHHRFTDLPDLLRPGDVLVVNTSATLPAAVPVTDSPLTVHFSTECDDGSWLVELRRNRGKATEPFPEGSSGRRYPLPGGGAVVLGEPWSAGRLWRAEVVTGGARSVQAFLYAHGRPIRYGYVRRRWPLAAYQTVFGVSPGSAEMPSAGRPFTDVLVTRLVTAGVLFAPVLLHTGVASPEAHERPYPERFVVPPASRRLVARARADGGRIIAVGTTAVRALETAWDGPAQGWTELVITPDRGLRAVDGLLTGFHEPRASHLDMLAAVAGTPLLERCYAEALDARYLWHEFGDVNLLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5VXX5</accession>
    <name>U5VXX5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362169.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17850"/>
    <dbReference type="EnsemblBacteria" id="AGZ41848">
        <property type="protein sequence ID" value="AGZ41848"/>
        <property type="gene ID" value="AFR_17850"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17850"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3621"/>
    <dbReference type="eggNOG" id="COG4106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090249_0_0_11"/>
    <dbReference type="OrthoDB" id="1739932at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="49"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="254" mass="27477" checksum="6F34EB3390AD7843" modified="2014-01-22" version="1">MTHYLADSAAAWQESWNLQQEAFLPDREHRFTTMLDAVGAVTDDAPPRVLDLAGGTGTISLRTLARFPGAEVTVVDQDPVLLALATASLGDRATIIAADLGDPSWTAALPHRRYDAVLTATALHWLPPERLTTLYGEILQVLRPGGVFANADHMPDEGLPGLTKRLMTRADTRRETRYATGAALSWPGWWSKVAADPQLAPLFAEREKIYPAAHGAAEWTPPAAWHLDALRAAGFTETGLLWRGAADATVAAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY09</accession>
    <name>U5VY09_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_16920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16920"/>
    <dbReference type="EnsemblBacteria" id="AGZ41662">
        <property type="protein sequence ID" value="AGZ41662"/>
        <property type="gene ID" value="AFR_16920"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16920"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3433"/>
    <dbReference type="eggNOG" id="COG1071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016624">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017596">
        <property type="entry name" value="PdhA/BkdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF41">
        <property type="entry name" value="PTHR11516:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03181">
        <property type="entry name" value="PDH_E1_alph_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="E1_dh" evidence="3">
        <location>
            <begin position="40"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="356" mass="38181" checksum="7EFA21B565280E72" modified="2014-01-22" version="1">MPSPEPVTLIGVDGQATGDPGHAVPDDTELVRGLAALITARRLNDQAYALVRQGRLAVYPSSHGQEACQVAAAQVLTGDDWLFPTYRDTVAAVGRGVDPVEALTLLKGDWHCGYDPYDHKVAPHATPLATQLPHAVGVAHAARLRGEPTVVMAMCGDGGTSEGDFHEALNFAAVLKAPVVFFVQNNEYAISVPLARQTAAPSLAHKGIGYGVPGERVDGNDLAALLAVLGAAVERARAGGGPQLIEAHTYRMQAHTNADDATRYRKDDEVAAWVERDPITRLERHLRERGLVDDEQLAGFAATAEQLAAHVRDGLHRDIEPDPAKLFDHIYAEPTPQLREQAALVADELDREGALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY63</accession>
    <name>U5VY63_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_11410</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023360560.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11410"/>
    <dbReference type="EnsemblBacteria" id="AGZ40571">
        <property type="protein sequence ID" value="AGZ40571"/>
        <property type="gene ID" value="AFR_11410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2326"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_11"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ40571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="402" mass="43673" checksum="906F2C724A631293" modified="2014-01-22" version="1">MADIVLDNVTKSYPDGTVAVHGIDLEIADGEFIILVGPSGCGKSTTLNMIAGLEDITSGELRIAGEKVNDKAPKDRDIAMVFQSYALYPNMTVRENMAFPLKLAKMDKAQIDKKVEDAAKVLELTEYLDRRPANLSGGQRQRVAMGRAIVRSPKAFLMDEPLSNLDAKLRVQMRTQVSRLQKQLGTTTVYVTHDQTEAMTLGDRVVVMRGGYIQQVGDPQTLYDHPKNLFVAGFIGSPSMNFLPATVASGELQTPLGNLPLGDRMRRMLEAGDAPDQLVLGIRPEHFEDASLVEDHQRSHGFEFTAPVDLVESMGSDKYVYFSIEGGAAAAADLEDLAADAGGADLPADAANLVTRLSAESRIREGHDAKVWLNLDKIHLFDPEDGRNVTLHEGRAAGVAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5VY90</accession>
    <name>U5VY90_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Group 1 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_17905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362191.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_17905"/>
    <dbReference type="EnsemblBacteria" id="AGZ41859">
        <property type="protein sequence ID" value="AGZ41859"/>
        <property type="gene ID" value="AFR_17905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3632"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_4_11"/>
    <dbReference type="OrthoDB" id="948440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="4">
        <location>
            <begin position="56"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="3">
        <location>
            <begin position="203"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ41859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="385" mass="41162" checksum="846F82F4EB024343" modified="2014-01-22" version="1">MRIAHFSDCEPARADGISASAGLAVALLREAGHHVDDYRPGPFFRGDRPVELRSVPVPGRSVRMAMPWSRAGPPADLVHVHTTGPVGLAGFRLAAKHRIPLVLTWHTDLLAYAEHFPEIPIGAAWCAVRLGLGWSARETLELAHRGRRRHTRLLVLGQAMMAASSLVIAPSAKTAAGLRAFGTLPPVRVLPTPAVLPPDGGEDLRAVLGIPHDAEVVLSVGRVTAEKNPVLLLEAFARVRRLRPYAKLVLLGARQRRRAVRALAGTLGLEDAVLLVPPVPHDRVAAFYRMADVLAFASTTDTQSLVIGEAEAAGLPVVSADPELGRLTCGPGPESFAAAIVRVLDDPGLRDRARAAGLAAARDRSPERYLEQLLAAYAEARSLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYF4</accession>
    <name>U5VYF4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_11935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ40676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_11935"/>
    <dbReference type="EnsemblBacteria" id="AGZ40676">
        <property type="protein sequence ID" value="AGZ40676"/>
        <property type="gene ID" value="AFR_11935"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_11935"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2430"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006850_4_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="827"/>
            <end position="891"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ40676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="891" mass="95680" checksum="7909563622A39A54" modified="2014-01-22" version="1">MAEVLVGRDDELERIAEVVSRAAGSAGGALVLRGPAGMGKSALLRAGGDLARERRMRVLSVTGVQAEGRLPFAALRHLMEPLHRKMRAGEPHTAQGPAGEPHTAGLSALELLSSAGSEQPVCLIVDDAQWIDRQSWEALSFLGRRVEDEPLCLLVAVRDGDEAKDRLAGAGLAEVPVGPLPDGAAESLLDRVAADLSPALRRVVLTEAGGNPLGLIELSASAGRLDPTGVPAALPLTARLEQTYSTVVAGLPAPTRELVLMAAVDDSDQLDEVLAAATASLDTLQPALAAGLLTTDGVTVRFRHPLIRSAVQQTATVAERLATHAALARVLPPDDDRHAWHSAAATVGTDEKLSRRMAELAARAGRGGAHDTAIAAWQRAAELTGDLHSRVDRLMWAGMEAMKRGDRKRIEGLVRAIGGYELDESQQVRLLWMREAVLGEGWSGGTRHARLVGVVERIIEQGDDELALDALMMWAMRNWWEPPPPDRRAGIAALAERLRVPAGDTRLIRVLALNAPLERAATVVDRLAEKMQSPPSHPDRLFELAEAATAVGDHPSAALLLTDSIAGARFNGNFSAVLFGLKAQAWVAAHTGEVRLAVVAGEEAQRLALEFGDFVGAISAQLCVGLAEALRGNTAAAIANADEAERQLHSGGRHPFRSVLTAVRGVAMLAAGKPVDAFEHLRRIFDPQDEAFHPYVRLSELAHLAEAARLAERLPELAPIVAECRILAASAPLPVLTVNLRYATALLAADPEQTLRDALKSDLASWPFERARIQLALGEELRRQRRPADARPILRAAWQTLTALGVTPWAERTRRELRATGETVNRAEDRTGRLTAQELQIAQLAARGLSNQEIARQLYVSPRTVSTHLYRIFPKVGVRSRAGLAEALRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VYM6</accession>
    <name>U5VYM6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative two-component system sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_18580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18580"/>
    <dbReference type="EnsemblBacteria" id="AGZ41994">
        <property type="protein sequence ID" value="AGZ41994"/>
        <property type="gene ID" value="AFR_18580"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18580"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3771"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="3">
        <location>
            <begin position="211"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="397" mass="42705" checksum="4BFC235A801A5AB7" modified="2014-01-22" version="1">MPGDPSDITVPQNHSGNDTAKAPEVSFTNRALGFVAGILTALLEAGYFVVVGAVLGPAMLWPRTRAAAIRRFLVGVRWLTELELRRRSRFFDDLFPVERDDQKALPYLTSHLAAGLLSAIVVGLLGFGLVLVVLVTRGMIRGSLGIWDLLAQVLLGGVLLFLDIQGIITLAGLDGRLARRYFGPSEREMLKRRIAELSASRAGIVRAVDSERQRIERDLHDGVQQRLVALAMLLGRARRHRDPVEAGRLMEQAHRESEEVLVELREVAWRAYPSALDNLGLPEALAGVAERSSIPVRVRIDLDTPLPRPVETATYFVVSEAVTNAVKHSGAAAIGVAIERRSGCVHVRITDDGAGGADPAGSGLSGLGLRVAALDGRMSVGSPPGGPTEITVEIPCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ13</accession>
    <name>U5VZ13_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tap domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_19705</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19705"/>
    <dbReference type="EnsemblBacteria" id="AGZ42213">
        <property type="protein sequence ID" value="AGZ42213"/>
        <property type="gene ID" value="AFR_19705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19705"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013364_3_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665507" evidence="1">
        <location>
            <begin position="20"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="71"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ42213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="449" mass="47936" checksum="37CDDE2F13A48D37" modified="2014-01-22" version="1">MVLVTVAAGVTAVAPPASARPAPIDWRPCAADKDAQCADLTLPVDWSEPSGATFTLAVARRAAPESTRIGTLIFGPGGPGDSGVRRVVTGQSRFSDELRSRFDIVSFDPRGVGGSNPIKCPTKVPAPAVLKDQAEFDATRKANREFWDACRVLTGPVFDHADTGSTVRDLDALRAALGERSLTFHGSSYGTLLGELYAERYPGRVRAMVLESVVDHSQDTRGFLTTQAWALQDSFDAFVAWCDTTEACVLHGEDVRSIWAQQMARADDGTLGVSAFELAANAHDALRKPNYPALATFIDGLRDGGTASPASQRPVQAVFCADWSLPVRNYREYQRLLRAAAAEAPDVRLPAATFGLSDCLGWPKPVASPQHVLHVRTRTPLLLINARHDPATGYNWATEVARQLGRHGVLLTYAGAGHGSYGSTCMRSAVDAYLISAALPAPGTVCPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ35</accession>
    <name>U5VZ35_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADP-dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_19830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362610.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_19830"/>
    <dbReference type="EnsemblBacteria" id="AGZ42238">
        <property type="protein sequence ID" value="AGZ42238"/>
        <property type="gene ID" value="AFR_19830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_19830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4024"/>
    <dbReference type="eggNOG" id="COG2130">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_29_2_11"/>
    <dbReference type="OrthoDB" id="474248at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PTHR43205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="19"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="336" mass="35483" checksum="FA2EB368819E8D22" modified="2014-01-22" version="1">MSVRSTEVRLAARPHGWPTADTFAIAETELGGPADGQVLVRNLMMSVDPYMRGRMDDRKSYVPPFEVGQALDGGAVGEVIESAAEGFKPGDLVLHGLGWRSHALVDARGASKVDPSVAPLGAYLGVLGMTGMTAYAGLLRTAEFKPGDVVFVSSAAGAVGQVVGQLAKLKGAARVIGSAGSAEKVAFLTGELGFDAAFDYHDAPVREQLKQAAPDGVDVYFDNVGGDHLEAAISAMNVHGRITICGMISVYNATEPTPAPRNLAQLIGKRLTMRGMLVRDHRDLRDEFIADVAPLVANGTIRYHETVFDGLDQAPEAFLAMLRGDGLGKNLVRIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZ81</accession>
    <name>U5VZ81_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_18890</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18890"/>
    <dbReference type="EnsemblBacteria" id="AGZ42052">
        <property type="protein sequence ID" value="AGZ42052"/>
        <property type="gene ID" value="AFR_18890"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18890"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3837"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040020_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="22"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="406" mass="42116" checksum="D96AA8B964952D39" modified="2014-01-22" version="1">MYLSTITRPATSRRGLTLVGGNVLALGTVSLITDVSSEMVTAILPMYLVLGLHVSPLAYGVVDGVYTGATAVLRLAGGYLADRFRGRKAVAGFGYALSAVAKLGLLAAGRSMTAFGVVIAADRLGKGLRTAPRDALITLSVPQEALGRAFGVHRMMDSIGAFLGPLVALAVLAASAESYDAVFVTSFCIAVLGVLVLVLFVREQRTVTVAPPAALREALALLRDPPVRRLAGAACLLGLATVGDGFVFLLLQRREDLGIAWFPLLAVGTSLAYLLLATPLGVLADRVGRLPVVLGGYVALAGTYLLLFGPVGGWPLLVLALGLYGLFYAATDGILVALAGPLLPERLRTTGIALVQTGQALAYLVSSVLFGLAWQTFGPETASRLAAAVVVLAIGATLVVLRRRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZG9</accession>
    <name>U5VZG9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_14070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361159.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_14070"/>
    <dbReference type="EnsemblBacteria" id="AGZ41100">
        <property type="protein sequence ID" value="AGZ41100"/>
        <property type="gene ID" value="AFR_14070"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_14070"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.2854"/>
    <dbReference type="eggNOG" id="ENOG502ZDMN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010343_0_0_11"/>
    <dbReference type="OrthoDB" id="2096376at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="123" mass="12748" checksum="E980163D02070834" modified="2014-01-22" version="1">MPFTKLNKAGMIIALVLGVLDLIAPFTPTPDGEDGPPYAILVIDAVLGLITVIAVVIAWRTARRGAVRIAAGARIISAVTALPAFFVDVPAALQAAVGFFVVITIACVAMMLAPSRRPVPVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VZM5</accession>
    <name>U5VZM5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4397 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_20880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362820.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20880"/>
    <dbReference type="EnsemblBacteria" id="AGZ42448">
        <property type="protein sequence ID" value="AGZ42448"/>
        <property type="gene ID" value="AFR_20880"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20880"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4233"/>
    <dbReference type="eggNOG" id="ENOG502ZCRG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080657_0_0_11"/>
    <dbReference type="OrthoDB" id="1752332at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025510">
        <property type="entry name" value="DUF4397"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14344">
        <property type="entry name" value="DUF4397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665909" description="DUF4397 domain-containing protein" evidence="2">
        <location>
            <begin position="31"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4397" evidence="3">
        <location>
            <begin position="33"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ42448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="270" mass="27302" checksum="6063AAB8488B5168" modified="2014-01-22" version="1">MFLHRTAAVGAAALVAFGGLAAVTATPAFAATSQVSVVHGIPGQNVDVYVNGKKTLPDFKPGTVAGPLKLEEGSYDIVLTKPGDDISKAVLKVDDAEIPGGANISLVAHLGADGDPVLTPFANDVKKLDAGQTRLIVRHTAAAPAVDVRAAGEPVFENLTNPKEAKADLPAGTVKADVVLAGTDDVAIGPADLDLKEGTATIVYAVGSAEDKTLDLVTQSITGLHSAPGGVPSGSGGLADRGLGNTWYAVALAGLLLLGFGARRMVSTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0C9</accession>
    <name>U5W0C9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_15520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361445.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_15520"/>
    <dbReference type="EnsemblBacteria" id="AGZ41386">
        <property type="protein sequence ID" value="AGZ41386"/>
        <property type="gene ID" value="AFR_15520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_15520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3152"/>
    <dbReference type="HOGENOM" id="CLU_2730918_0_0_11"/>
    <dbReference type="OrthoDB" id="2050591at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ41386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="65" mass="7877" checksum="5909E0637CBD78CC" modified="2014-01-22" version="1">MPKDQNRFVSWFHRTFDSWFFRSFIGPAQTDNAVHGADAFAREQWKRDLEARKQYTRDKRAAARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0J3</accession>
    <name>U5W0J3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21745"/>
    <dbReference type="EnsemblBacteria" id="AGZ42619">
        <property type="protein sequence ID" value="AGZ42619"/>
        <property type="gene ID" value="AFR_21745"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21745"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4406"/>
    <dbReference type="eggNOG" id="COG0510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065747_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="31"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="342" mass="37320" checksum="893A2D036149D249" modified="2014-01-22" version="1">MLNPPQDLVDDPGAAQELLHNQTNGVTGGIWRVRRDGGSAILKICTPGRPEAAAHFQASEEPSHWNYWRRELLAYRTGFASGAFADAGLSAPALLGTEERPDGSVAMWLEDVAGTPGTSAGSADLGDVAYRTGVGQARWLDRPPAHEWLSRDFLRDYTTAQSGVGELDWDHPVAAEAWPPQLRADLRTLWERRDDVLAATDRLPRTLCHHDLWPMNLILAPRGPVLLDWAFTGPGAIGEDVANLTLDTFFDGFIDVSLIDEVVAAVGDGYRRGLGGVVDEATITRAIRLTGAAKYYWLAPRMLSALTQPRRGTVYDSRDTAAIFAGRAPVLSVVTRWARESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0M7</accession>
    <name>U5W0M7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative manganese-containing catalase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the manganese catalase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363040.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21995"/>
    <dbReference type="EnsemblBacteria" id="AGZ42669">
        <property type="protein sequence ID" value="AGZ42669"/>
        <property type="gene ID" value="AFR_21995"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21995"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4459"/>
    <dbReference type="eggNOG" id="COG3546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057467_1_1_11"/>
    <dbReference type="OrthoDB" id="1420351at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01051">
        <property type="entry name" value="Mn_catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007760">
        <property type="entry name" value="Mn_catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039377">
        <property type="entry name" value="Mn_catalase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05067">
        <property type="entry name" value="Mn_catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="305" mass="33207" checksum="B2F1B75F01A6B8CF" modified="2014-01-22" version="1">MFKHVDYLQFQAKPEKPDALLARKMQELVGGQFGEMTVMMQYLWQGWSCRVPGKYKDMIMDIATEEIGHVEMLTTMLARLLEGAPGEATEKAAAANPLLAAVLGGMSPQHAIVSGGSALPRDSEGTPWNTGYIAASGNLLTDFRSNVAAEAQSRLMTSRVYNMTDDRGVKDMLQFNLARDTYHQQQWLLGIEQLIEDGFVEHGIENSNGEDEKDFANHTFYSFDPESKAGEGRWARGPALIGKEEIEYVPDAVPLTDDKPLGPAPDPKLYVTYAGDMGKGKPGTGAGAHAQGVANVVNKIKGALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0S2</accession>
    <name>U5W0S2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">EmrB/QacA subfamily drug resistance transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_22915</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22915"/>
    <dbReference type="EnsemblBacteria" id="AGZ42853">
        <property type="protein sequence ID" value="AGZ42853"/>
        <property type="gene ID" value="AFR_22915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4644"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="473"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="17"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ42853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="503" mass="51532" checksum="8EBC69C8B776FF99" modified="2014-01-22" version="1">MGVADPTINDPRRRRAILIAVCVALMAVIASVSGLNVAQQEIALAFGASQGTVLWIINVYTITLAALLLPLGAVGDRWGRKPVLLTGLIVFGVASAAAGLATSSEVMIAARLLAGAGAAMIMPVTLAVITSTFPDEERSRAIGVWTGVAGGGGLLGMYLSAVLVDVASWRWLFVLPVALVVAAGILALRSVPNSRETTGQRFDITGSLSSVVAVVGLIFVLHEGPERGWTAPVTLLSLLVGVVGAAGFVMWELRHGAPLLDVRLFRKRGLATGSVALLAVFGVQAGIFVVLFPYLQAVLGWSGLRATLALMPMALLMMAASGLAPRVAARAGSRPTMAAGIFLGGAGLALMALFVSVDGGYLSVLPGMLAMGLGMGLTMTPATEAITSALPRARQGVASALNDVTREFGTALGVALLGAVLSAGYRGAIDARLDDGTAATAREGIATALAAAEGRPALVRAAQESFVDGWQQAMWAGVAVMTVLFVYVLVGGRNSVPVTTEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0U5</accession>
    <name>U5W0U5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_22445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22445"/>
    <dbReference type="EnsemblBacteria" id="AGZ42759">
        <property type="protein sequence ID" value="AGZ42759"/>
        <property type="gene ID" value="AFR_22445"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22445"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4550"/>
    <dbReference type="eggNOG" id="COG2182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_12_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665562" evidence="1">
        <location>
            <begin position="21"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="430" mass="46387" checksum="5549BAB359D2E578" modified="2014-01-22" version="1">MSLAAGALLLAASLGLTACSDSGSGDEGADAKTLTLWHYEGPTSAMGIAWNRAIELFKASHPGVEVKFEEKSFEQIQQNAGMILNSDSAPDILEYNKGNATAGLLSKQGLLTDLTEQATQRGWDKALSPSLQTTAKYDGDGIMGSGKIFGIPNYGEYVMVYYNKDLFAKNKIAVPTTLAEFEAAMDTFAKAGVTPLSVGGAEYPAQQILYELALSKANRAFVDNYQLYKSKVDFHGPELTFGAQTFADWVAKGYIAKNSAGIKAEDMGVAFTQGKFPIMISGSWWYGRFTSEIKDFQWGTFLFPGNSLHPGSSGNLWVVPEKSKAKSLAYDFIDITMKPEIQDLLGNNGGVPVAADPAKITDPKNKELIDNFAKISSSDGLAFYPDWPAPGYYDVMVGGVQGLINGSKTPQQMLDEVAKPYDDNLADLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-12-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W0U8</accession>
    <name>U5W0U8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lysine 5,6-aminomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_21810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_21810"/>
    <dbReference type="EnsemblBacteria" id="AGZ42632">
        <property type="protein sequence ID" value="AGZ42632"/>
        <property type="gene ID" value="AFR_21810"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_21810"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4419"/>
    <dbReference type="eggNOG" id="COG0274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_517447_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015130">
        <property type="entry name" value="Lys-AminoMut_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037086">
        <property type="entry name" value="Lys-AminoMut_asu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09043">
        <property type="entry name" value="Lys-AminoMut_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lys-AminoMut_A" evidence="1">
        <location>
            <begin position="7"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ42632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="520" mass="56502" checksum="5F571801FD0E3D44" modified="2014-01-22" version="1">MVVVDKLGLDPRVVKRARALAAKAGQPVVDLARSHTTVSVERAVLRLAGVSGADSDGIPWVNRLVDAVRADVGLGQGVAVPVFHALASQGLDDVTVLAQKAAAGAVTFGVPERKAEVTAARRAARRATNVGLKIVDRRRVERERLVKRLGDPEQRPWIYLIVATGDIYEDIPQAQAAARAGADVIAVIRSTGQSLLDYVPEGATREGFAGTYATQENFRLMRAALDETSKELGRYVRLTNYASGLCMPEIAALAGMERLDMMLNDSMYGILFRDINPIRTFVDQRFSRQVHARAGIIINTGEDNYLTTADAVEAAHTVTVSQLLNEFFAHEAGLENWQLGLGHAFEINPELPDSFRLELAHALLARELFPDAPLKWMPPTKHMTGDVFRGNLLDGFFNLAGALTGQGILLVGMMTEAVVTPWLSDRDIALQNVRYVLNGAGKLHEDFVPAPGGFIRSRAHEVLGEAIDLLEKVVDDTMLTAIAEGTFGIMKRPADRGKGLDGVAKHEDGYYNPVTEALEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>U5W154</accession>
    <name>U5W154_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative phosphoenolpyruvate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_16715</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_16715"/>
    <dbReference type="EnsemblBacteria" id="AGZ41621">
        <property type="protein sequence ID" value="AGZ41621"/>
        <property type="gene ID" value="AFR_16715"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_16715"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3389"/>
    <dbReference type="eggNOG" id="COG0574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3848">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005950_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPDK_N" evidence="3">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="PPDK_N" evidence="3">
        <location>
            <begin position="66"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="2">
        <location>
            <begin position="799"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ41621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="886" mass="92236" checksum="8140E18F37BD076D" modified="2014-01-22" version="1">MQVLALADVTAVTALPGGVTGLTGGVVGLVGGKAAGLGALIEQGFRVPPGFCVTTEAYRKGVLPEAEILAAYGKLGGGPVAVRSSATTEDQPDASFAGQHDTILDVTGPGALLAAITRCWESLHSPRATAYRKAHDLQTDTVRMAVVVQRMIDASVAGVLFTANPLTGSRTEMVVDAAPGPGSAVVDGTVQADHYVLGASATPAGGCLSPEQLAELQAVGERLPGEVDVEWAFDRSGTLWLLQSRPITTLFPLPPATPDLRVYFEFGHVQGMLQPVTPLGLSTLSDRIAAMLAALGVRAEIVDIGGRLYGDLTDLARHPATRKRLIKLMAVDFGPRAQAAMEWVLQDPRFAPDAAAARPGATMPSLATAARGVSGVVGALARPEKARRRLLQAVENLRLASAPPDHLQPLPAASGADGLLPAVSGTRELPPAVPGDRGLSPAVSDARGLSPAVSGARGLAAAVSGARGLAAAVPGGLEVAAERLRFVRRQDAPDGADAITWTIVAGMLAAAAPEQLLKGLAGPDEIHAVLAGMPHNVTIEMDLALWRLSQQVGEHRDLVLTTPPTELAERFLAGTLPEIGLTAFLTRYGHRGAAEVDLGVPRWDENPAPVLAMIANYLRVQDPEQAPDVRFARAATTAEATLDDLVARARRRRPVRGRLTGFLLRRARALAGLRESGKFAGLYSLREMRRQLLLIGADLATAGRLADRDDIVFATLGEVHAAVHDNLDLRAVITTRKAVHQRELRRRTVPVALLSDGTDIESLLTAGPAPDDALRGVGASAGRATGRARVVLDPATAHLEPGDILVTSTTDPGWTPLFLTAAGLVTETGAIMAHGPTVAREYGIPAVICVPAATTRITDGQLITVDGAAGTVTTADQSPNQSVASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1C5</accession>
    <name>U5W1C5_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enolase</fullName>
            <ecNumber evidence="8">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">eno</name>
        <name evidence="14" type="ORF">AFR_22735</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell surface</location>
        </subcellularLocation>
        <text evidence="8">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="8"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363304.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22735"/>
    <dbReference type="EnsemblBacteria" id="AGZ42817">
        <property type="protein sequence ID" value="AGZ42817"/>
        <property type="gene ID" value="AFR_22735"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22735"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4608"/>
    <dbReference type="eggNOG" id="COG0148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_11"/>
    <dbReference type="OrthoDB" id="533698at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Enolase_N" evidence="13">
        <location>
            <begin position="4"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase_C" evidence="12">
        <location>
            <begin position="139"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="364"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 9">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="8">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AGZ42817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="419" mass="44483" checksum="B965F1EF998EF7A0" modified="2014-01-22" version="1">MTVISRVTGRQILDSRGNPTVEVDVELEDGSLGRAAVPSGASTGSHEAVELRDGDPSRFHGKGVRRAVAAVNGEIAAAVTGLPAEDQARIDQTMIDLDGTPDKRRLGANAILGVSLATVKAAALAHRQPVYRYVGGAGARVLPVPMMNIINGGAHADNPLDFQEFMIAPVGAASLSEAVRMGSEVFHTLRKSLAAAGHNTNVGDEGGFAPHVVTADEALEFVLGAVEQTGYRPGTDITICLDPASSEFYSDGVYDYAGEGRKRTVEEHVDYLRGLLDRYPISSIEDPMAEDDIAGWQHLTRLAGDRVQLVGDDVFCTDVTRLRAGIEGGYANAILVKVNQIGTLTETLTTVDAAHKAGYRVVMSHRSGETEDTTIADLAVAVGCGQIKTGSLSRSDRTAKYNQLLRIEEELGDSARYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1G5</accession>
    <name>U5W1G5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_17390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ41756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023361986.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ41756">
        <property type="protein sequence ID" value="AGZ41756"/>
        <property type="gene ID" value="AFR_17390"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_17390"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3525"/>
    <dbReference type="HOGENOM" id="CLU_2646302_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ41756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="76" mass="8041" checksum="0A5A013C9E2E6275" modified="2014-01-22" version="1">MDFSATSILLGLVVIAAALGGGLAIYLGLRGEEPAVEEPGPADTGRHHVPEWLLDGDTRRLGPDVVQRSRADQHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Q7</accession>
    <name>U5W1Q7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative taurine catabolism dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_24410</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363738.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24410"/>
    <dbReference type="EnsemblBacteria" id="AGZ43148">
        <property type="protein sequence ID" value="AGZ43148"/>
        <property type="gene ID" value="AFR_24410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4945"/>
    <dbReference type="eggNOG" id="COG2175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036005_2_1_11"/>
    <dbReference type="OrthoDB" id="1742732at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD" evidence="3">
        <location>
            <begin position="11"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ43148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="286" mass="31501" checksum="97CEE638E61768D7" modified="2014-01-22" version="1">MTATFVKLDLEALGPRFGSVVHGVDLATATDDEIVAVRAALVDRKVLFFRDQSLDDDGQVGLGRRIGELTASHPVVGGVDAAHPEVYALDSTDNGFADVWHTDVTFVRRPPLGSILRAVTLPPSGGDTNWADAQLAYESLTPAVRTLVDGLTAVHDGTREFGYYLAQRRNGQGNEWDGEVVTRLDPVEHPVIRVHPETGRKGIFVNPGFTSHIKGVSEFESRAILDLLYAHLTKPEHIVRHRWRAGDVAMWDNRSTSHYANRDYGTDRRVMHRITLRGDIPVGPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1T1</accession>
    <name>U5W1T1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24560"/>
    <dbReference type="EnsemblBacteria" id="AGZ43178">
        <property type="protein sequence ID" value="AGZ43178"/>
        <property type="gene ID" value="AFR_24560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4976"/>
    <dbReference type="HOGENOM" id="CLU_862303_0_0_11"/>
    <dbReference type="OrthoDB" id="1335240at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004665600" evidence="1">
        <location>
            <begin position="27"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="322" mass="32532" checksum="ECCB46C62AAF9FF3" modified="2014-01-22" version="1">MLAAGAVAALLVVSGCSDSGSSDDKAAAVGQQAAAAAAGPPVISEADAKALMTKITAANNKANLALDPEQVGGYEAEASLASDAAGLISYVGRPLGEFDYELETLLAPSSTAGADFFVADAVVGSATQHYPLVLAKAGADWKVVHSAPLEVPLPELQKGAAGSVEVVDRADPAKTLLISPEQVAQEHATALGTAGGKGPFAGDATSAKVVESDQAREQIRFEDGAAPDALDIKTTVTPYPVRALRTKDGGALVAYSTQTEFVATKAGAEADLGPILTKLGGRTAPGKVSVTILNMWWTKVPAMGKGDKVTVLGGTNQPVSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W1Z3</accession>
    <name>U5W1Z3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_18635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362378.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_18635"/>
    <dbReference type="EnsemblBacteria" id="AGZ42005">
        <property type="protein sequence ID" value="AGZ42005"/>
        <property type="gene ID" value="AFR_18635"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_18635"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.3782"/>
    <dbReference type="HOGENOM" id="CLU_1544347_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ42005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="173" mass="18347" checksum="DC9196DE5A1B4C8D" modified="2014-01-22" version="1">MVILRGVQVPGHVVHSDGFRATTGDLITVALGFACLRREAAAADEPSRVTSEEGRDCELVGVSQSYATTIFADGWLVEAAGILLYAQDLGPAADLPPPGTRVRAQGSLAVAEAYESAGFEPAADLLARAEQQWLVQRIVRRSGPARRVVEAIDSFRTGSDDYLLDLAGSGHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2J9</accession>
    <name>U5W2J9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_24855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363827.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_24855"/>
    <dbReference type="EnsemblBacteria" id="AGZ43237">
        <property type="protein sequence ID" value="AGZ43237"/>
        <property type="gene ID" value="AFR_24855"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_24855"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5035"/>
    <dbReference type="eggNOG" id="COG3022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071581_0_0_11"/>
    <dbReference type="OrthoDB" id="1361974at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005583">
        <property type="entry name" value="YaaA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30283">
        <property type="entry name" value="PTHR30283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03883">
        <property type="entry name" value="H2O2_YaaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="268" mass="28123" checksum="BD1A960134204EE7" modified="2014-01-22" version="1">MLILLPPSEGKTAPAAGEPADPADLWLPRLAPARRRVLAKLTSVTKRTSARGVADSLAILGLSAGQQGEIARNAALLTAPAAPAAEVYTGVLYEALGPAGLAPGARAWVDERAVVFSGLWGVLRLTDRIPAYRCSVGVTLPVIGGLTAYWKKALTPVLDRAAADGPVLDLRSGAYAAMWTPSGGLAGRTAALRVLHERIVGGEPRRSVVSHFNKATKGRLVRSLAEAGAAPASVDDLLDVLRDLKYTVEERPVAAGRPRQVDVIVREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W2Y1</accession>
    <name>U5W2Y1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">AFR_20045</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20045"/>
    <dbReference type="EnsemblBacteria" id="AGZ42281">
        <property type="protein sequence ID" value="AGZ42281"/>
        <property type="gene ID" value="AFR_20045"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20045"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4067"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="20"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="284"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ42281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="557" mass="58567" checksum="4F1479E2D623EA6A" modified="2014-01-22" version="1">MRGPLPYLWWLVRRQKLRIATGALLGSLWMVGLTLPPYLLSQAIDRGLQRRDPVALTAWVAVLFAVGAINAVLAILRHRTMTKIRLDGGFRTVRAVIAHATRLGAALPRRIGAGEIVTIGIADVWVLAQALTVTGPGFGAAVAYVVVAVLLLEISPLLAVVVLAGVPVLAIVVGPMLGRLQRAGARYRERQGALADRLVDIIGGLRILNGLGGKDAYAHRYRRDSQRVAEEGYRVGAVTSWIQALGVGLPALFLAVVIWLAARMAAQGDITVGELVAVWGYAAVLVVPVAFFIEGGFDVSRALIAGRRVVDFLRLPVDPRPSGQGPDAPAVLRDPESGVVVIPGRLTALATARPADAVAVVDRLGRYGPTAAVWGGARLEGLAVRDRILVADNEAYLFTGALGEVVAGRSEPDDEAVARALTAASADDIVDGLPDGTASLVDTHGRNLSGGQRQRIRLARALYADPEILLAVEPTSAVDAHTEAAIAERLPAARAGRATLVATTSPALLDRADVVHYLVGGRLAATGTHRALLDTEPGYRALVTRAAGEPATTESVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W364</accession>
    <name>U5W364_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_20520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023362748.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_20520"/>
    <dbReference type="EnsemblBacteria" id="AGZ42376">
        <property type="protein sequence ID" value="AGZ42376"/>
        <property type="gene ID" value="AFR_20520"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_20520"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4161"/>
    <dbReference type="eggNOG" id="COG1176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_18_5_11"/>
    <dbReference type="OrthoDB" id="1488093at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="67"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ42376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="291" mass="31417" checksum="DF7B5A6E868B13FF" modified="2014-01-22" version="1">MVALRHRLAERGIDRQLLLLIPAAVFVLLLFIYPFLYGVGLSFQPVEGGALGAYREFFTDAYLRDTIGTTLQLSLPAALLNVLASVPIAYRMRGRFRGKRLLTTVLVVPITLGTVLTAQGLLNFLGPTGWFNRTLLSTGLADAPVRLTNNYWGVFFSLVITGFPFAFLLVLSYLSGIDPSLERAAATLGAGRWQRFRRITLPLLAPGLATTFCLTFVLAFSVFPSAVLVGDPSGTTRVMSIAAYDAAYVRYDYPLASAVAVLMGLVELIVIGAVLGWRALLYTGSTAGGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W417</accession>
    <name>U5W417_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acyl-CoA dehydrogenase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_22275</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ42725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023363096.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_22275"/>
    <dbReference type="EnsemblBacteria" id="AGZ42725">
        <property type="protein sequence ID" value="AGZ42725"/>
        <property type="gene ID" value="AFR_22275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_22275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.4515"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_2_11"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013107">
        <property type="entry name" value="Acyl-CoA_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08028">
        <property type="entry name" value="Acyl-CoA_dh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="3">
        <location>
            <begin position="10"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="2">
        <location>
            <begin position="113"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_2" evidence="4">
        <location>
            <begin position="234"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ42725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="38721" checksum="49C3578B456B1CF1" modified="2014-01-22" version="1">MSELDDVTAALATTAAKYDATGEFPAEGLRVVHEAGLLTATVGEAYGGRGAGVSELARILLALGRGDPSVALIASMTLFPHLLQARHPHWPADLYASVLARPGPVLLNNCRVEPELGSPARGGLPATTARRTAEGWSISGRKRFVTGAAGLTYFLVWAATDEPAPRVGTFVVPGDSSGVEVIPAWDQLGLRASGSHDVAFADVPVKRDHVLGLVPADAKAQQDNLAGGALHLPLAALYVGVARAAQQAFHRFAHERVPANLGRPVASTDRFRSAAGEIEVLLTGAERLILDATGRFDRGEDVPGTAALGARVLASRHAVAAVTLAVRLLGNPGLSRADPLERHFRDVQSALVHAPQEDTALLVIGTAALAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W431</accession>
    <name>U5W431_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative SnoaL-like polyketide cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28560"/>
    <dbReference type="EnsemblBacteria" id="AGZ43973">
        <property type="protein sequence ID" value="AGZ43973"/>
        <property type="gene ID" value="AFR_28560"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28560"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5790"/>
    <dbReference type="eggNOG" id="COG5485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100997_5_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07366">
        <property type="entry name" value="SnoaL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="157" mass="17664" checksum="3FFF31575491748E" modified="2014-01-22" version="1">MKIMASGELTELHEVIHPDAVNREATAEPPDCRVQGPAAFAATARWLRSAFAELAFDIHETAASDDLVVVHCTMSGRQVGPFVTYDEHGRVAQAFPATGRQMAVTHTHWTRMRDGLVFEHWANRDDMSQALQLRWVPPTPLYLCRMALAKRQASRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4L2</accession>
    <name>U5W4L2_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_29410</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560480.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29410"/>
    <dbReference type="EnsemblBacteria" id="AGZ44143">
        <property type="protein sequence ID" value="AGZ44143"/>
        <property type="gene ID" value="AFR_29410"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29410"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5961"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_11"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="223" mass="24098" checksum="83609095351B4DB2" modified="2014-01-22" version="1">MTVLAVRHASMVYPGPVTALDDVSLSIEQGELLAIVGPSGSGKSTLLHIMGTLDRPTRGTVEIGGHEVSGLSDRRLSALRGRMLGFVFQQFHLTDGLTAAENVATGLLYAGVPRRRRRPLALEALARVGLEHRVGHRPNQLSGGERQRVAIARALVNEPALVLADEPTGALDTRNGHLVLDLLHRLHEEGTTIAVITHDRDIAGRLPRRVEIRDGRLIGDTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4S7</accession>
    <name>U5W4S7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_29710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560540.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29710"/>
    <dbReference type="EnsemblBacteria" id="AGZ44203">
        <property type="protein sequence ID" value="AGZ44203"/>
        <property type="gene ID" value="AFR_29710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6020"/>
    <dbReference type="eggNOG" id="ENOG502ZWK1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166740_3_0_11"/>
    <dbReference type="OrthoDB" id="1925819at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019270">
        <property type="entry name" value="DUF2283"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10049">
        <property type="entry name" value="DUF2283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="7590" checksum="8F2BFB67C505043A" modified="2014-01-22" version="1">MRLTYDDEANAAYLEIEDIAEGTAVENVVVERPGRGDIILDFDAAGRLLGVEVIGATALVRTTALDAAERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5W4Y0</accession>
    <name>U5W4Y0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GAF sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_28955</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28955"/>
    <dbReference type="EnsemblBacteria" id="AGZ44052">
        <property type="protein sequence ID" value="AGZ44052"/>
        <property type="gene ID" value="AFR_28955"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28955"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5867"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_412658_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="37"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="200"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="365"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="2">
        <location>
            <begin position="619"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="713" mass="74871" checksum="94E9ECE35390C183" modified="2014-01-22" version="1">MILPRRIRLVRSVGTPSPADGQAALRRITSLVAAGEGPQRVFEAVTTEASALLGDALIALTRFEGGGTDSVVVAQTGDHVPVGARLHLEGADGVAARMWRSGRAERIDDYTGIAGTTAADLGVRAVVAVPVIVDGSLWGALSVSSRTGPLPAGTEARLMTFAQLVAAAVVSAAARESFLTLAGEQAALLRVAALVAHQAGQTEIFEAVATEAARLIQDEATTLVRYEGDRTFTVLAHCNGPVPVGTRYTVPVDDGGTSNEMMRTLKPARLDRYDLFPDRSYYCQDLGIGSSVSVPIIVNGRLWGALGAVNEGRRLPANTEERLGKFAELVASALANVEARAELEHFAARQAALRRVAELVARGAVLAEVFDAVATEASNILGESPADLFRYDGTHCTVVATCRSSVPVGFRSRADGDSAGARVMRARRPLRFTTLDDTESADHAREFDVGALVAVPIVVEGRLWGGLATATRGEPPPADAEDRLAEFAELAAAAIANAENKEKLRASRARVVATANEVRQRLQRDVHDGAQQRLVQTVLSLKLGLDAADRGQDTVEMMREALEHAERATVELRDIVHGILPVALARGGLRAGIDSLVTAAPIPVDLDLSAQPQERLPSDVEVTAYFVVAEALTNVVKHAGATRVRVTVAGAADQLVVAVIDDGVGGADPRGGSGLTGLADRVEALDGTLTLRSARGAGTTVQVILPTRTRAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5P0</accession>
    <name>U5W5P0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560851.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31290"/>
    <dbReference type="EnsemblBacteria" id="AGZ44518">
        <property type="protein sequence ID" value="AGZ44518"/>
        <property type="gene ID" value="AFR_31290"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31290"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6333"/>
    <dbReference type="eggNOG" id="COG2764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_11_1_11"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="11"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="140" mass="15127" checksum="ACF4BF12E1BC5423" modified="2014-01-22" version="1">MAETTEQPPPPQVWPTLRARDARALIKFLVDAFGFTETAVYGEGDHVAHAELAWPLGGGIMLGSHRDDPDDAWALRPGTFGAYVVTDDPDGLHDRAVKAGATITKGLAETDYGSRDFAARDPEGNLWSFGTYRGEPVSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5R8</accession>
    <name>U5W5R8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Elongation factor G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_31120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560818.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31120"/>
    <dbReference type="EnsemblBacteria" id="AGZ44484">
        <property type="protein sequence ID" value="AGZ44484"/>
        <property type="gene ID" value="AFR_31120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6299"/>
    <dbReference type="eggNOG" id="COG0480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_4_2_11"/>
    <dbReference type="OrthoDB" id="88967at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="19"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="716" mass="75712" checksum="015DF6B5E04D34E9" modified="2014-01-22" version="1">MTQKTQEKGLTAPVVTEPGRVRNVVLVGHSGAGKTTLIEALLAATGTISRAGTVADGTTVTDHDPAAVRQQRSVALSCAPVVHDGVKINLLDTPGYADFVGELRAGLRAADAAVFVVSAVDGVDSATVALWEECAAIGLPRAVTITRLDHPRADYEGTLQECQEAFGENVMPIYQPMLGDDGESIAGLLGLVTLKVLDYSQGYPPRAGEAEREHLTPIKDDRDLLIEGIIAESEDETLMDRYVAGELISTDTLVPDLEKAVARGNFYPVIPVCAATGVGLDALLDGIVNGAPSPLEHDLPVVTGVDGSPLPPLTCDPDGPLVAEVVKTTIDRHVGRVSLVRVFSGTLRPELTVHVSGHGLAERGHADHDADERIAHVYSPLGSQLREVASCIAGDICAITKSGSAETGDTLSGKDQPLLMEPWTMPEPLLPIAVVAKSRSDEDALAKNLARLVAGDPTLRLERNPDTHQLVLWCMGESHADVVLDRLRAGGVELDTEPVKVSLRETFGATATGHGRHVKQSGGHGQYAVCSIEVEPLPRGSGFEFVDKVVGGAVPHNYIPSVEKGVRAQLEKGIVAGYPVVDLRVTLVDGKAHSVDSSDAAFQTAGALALRTAAEQGRVALLEPVDEIVVRVPDTYVGAVMSDLSGRRGRPLGTEADEDGEHSLVRAEVPATELVRYAVELRALTSGTGTFSRVYARHEPMPAHLADAVRKEHAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-02-26" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>U5W5T8</accession>
    <name>U5W5T8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ43360">
        <property type="protein sequence ID" value="AGZ43360"/>
        <property type="gene ID" value="AFR_25480"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25480"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5163"/>
    <dbReference type="HOGENOM" id="CLU_1954884_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="128" mass="14606" checksum="D3233C9164C9ED9A" modified="2014-01-22" version="1">MFGMPETRITRRERRAIAAEWRQVPFRTRLEVARLAKQGERHPDDGVDAVAVRVSRMALEEPSSSFFHTTAFGVLLTLTLLAIAVLWDDTFPRWCAGLGGSACFVLLVFNWDLKRDARRILAVPRHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>U5W637</accession>
    <name>U5W637_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_31915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31915"/>
    <dbReference type="EnsemblBacteria" id="AGZ44643">
        <property type="protein sequence ID" value="AGZ44643"/>
        <property type="gene ID" value="AFR_31915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6460"/>
    <dbReference type="eggNOG" id="ENOG50329W0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064711_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="25197" checksum="BE0D2A78FB32132B" modified="2014-01-22" version="1">MFIVETAATVWMIVLLAVAAATAILAVPRRPSRAAALPAADPYAEQVALAAEQAAAIARRRRADWTRAQEEVDAAWAAFDVADRDARRYAAAAAFPVFKQRRTRAELADRERHLHRSATAYCRRRELSIAQLNEVLAHRGGWNPRRHPVAQEAALRAAVREHRFADYQAATGRERQAWQDAERAAATVRSLRAEALQVRARAGRDVHYEEQWTSVRPAKARLAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W654</accession>
    <name>U5W654_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_25835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364126.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_25835"/>
    <dbReference type="EnsemblBacteria" id="AGZ43431">
        <property type="protein sequence ID" value="AGZ43431"/>
        <property type="gene ID" value="AFR_25835"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_25835"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5237"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062658_5_2_11"/>
    <dbReference type="OrthoDB" id="1831818at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="175" mass="19703" checksum="375774B325F78E51" modified="2014-01-22" version="1">MKQVSTRVVYSNPWMTVREDQIAHPDGTPGIYGVVEKRDFALVLPRWEDGFWLVEQFRYPIGRRAWEFPQGGWGHSATGTSAELARTELAEETGLRARSLVHLGHLYEAYGYSTQGFDVYLAEDLTEGDTAREQSEQDMVHRRFTDAEIAEALRSGRIVDAPSLAALTLYGLHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6B4</accession>
    <name>U5W6B4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">2-hydroxy-3-oxopropionate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">AFR_26145</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HIBADH-related family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26145"/>
    <dbReference type="EnsemblBacteria" id="AGZ43491">
        <property type="protein sequence ID" value="AGZ43491"/>
        <property type="gene ID" value="AFR_26145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5299"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008679">
        <property type="term" value="F:2-hydroxy-3-oxopropionate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046487">
        <property type="term" value="P:glyoxylate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006398">
        <property type="entry name" value="Tartro_sem_red"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01505">
        <property type="entry name" value="tartro_sem_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="4">
        <location>
            <begin position="7"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_binding_11" evidence="5">
        <location>
            <begin position="168"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ43491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="297" mass="29592" checksum="700C9AC1C6FED852" modified="2014-01-22" version="1">MFMPDAIGFIGLGIMGSPMAANLVKAGFDVAGHTRSRAGLDRLAAAGGRPVTGLAEAVTGADVVITMLPDSPDVEQVILGPDGVLAHARPGTLLIDMSTIRPRTARAVAAAAADRGLRPLDAPVSGGEQGAVEGVLSIMVGGAAADFEAAGPVLSALGTTIVHVGPSGAGQTVKAANQLVVAGTIQLLAEAIVLLEASGVDAHAGLQVLAGGLAGSTVLDRKGAGMLARTFTPGFRSDLHHKDLGIMLDAAREAGAVVPVGALLGQLMAALRAQGDGGLDHTALLLATERMSGRTHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6E9</accession>
    <name>U5W6E9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_32625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561121.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32625"/>
    <dbReference type="EnsemblBacteria" id="AGZ44783">
        <property type="protein sequence ID" value="AGZ44783"/>
        <property type="gene ID" value="AFR_32625"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32625"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6602"/>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107144_1_1_11"/>
    <dbReference type="OrthoDB" id="1794390at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030489">
        <property type="entry name" value="TR_Rrf2-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01332">
        <property type="entry name" value="HTH_RRF2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ44783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="151" mass="16397" checksum="899AA89DC093632D" modified="2014-01-22" version="1">MQISARGDYAVRAALSLAHAYPALMSAQAIAQDQNMPRKFLEAVLADLRRAGVVRAQRGAEGGYTLSQSPRDITIGQILRAVDGPLAGVRGLRPEETQYSGAAENLPNLWVAVRSAVREVVDEVSLAELISGRMPAHVRKLTTRPDAWQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6K9</accession>
    <name>U5W6K9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_26740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023364477.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_26740"/>
    <dbReference type="EnsemblBacteria" id="AGZ43610">
        <property type="protein sequence ID" value="AGZ43610"/>
        <property type="gene ID" value="AFR_26740"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_26740"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5420"/>
    <dbReference type="eggNOG" id="COG1801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046519_0_1_11"/>
    <dbReference type="OrthoDB" id="1068600at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="PTHR30348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="SSF117396"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="290" mass="33214" checksum="56554C8A74864FE4" modified="2014-01-22" version="1">MGEIQVGTASWTDKTLLDSGWYPQTADNPEKRLAYYAKQFPVVEVDATYYGPPAEQTTRLWAERTPEHFTFNIKAFSMLTGHPTKVSAIYKDLRPDTDKKNVYPGDLPPQAYEEIWTRFLSALDPLVEAGKLGALLFQFPPWWGIRRSNKDYLLEVAARCRPLRPVFEFRNASWFDGDNAGETLDFLRKHNLPLVCVDMPQGHKSSVPPITAATADDLAVVRFHGHSDKWTSKDIHEKFGYNYSKKELREWAPKLRALADETRTTQVFMNNCYTDYAQRNATTLIELLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W6W6</accession>
    <name>U5W6W6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_33155</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33155"/>
    <dbReference type="EnsemblBacteria" id="AGZ44889">
        <property type="protein sequence ID" value="AGZ44889"/>
        <property type="gene ID" value="AFR_33155"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33155"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6709"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="147"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ44889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="214" mass="22485" checksum="CFB2CAF2F30DD394" modified="2014-01-22" version="1">MIRLLIVDDDAMVRTGLRLILGGEHDITIVGEAADGREAEAQVRELSPDVILLDIRMPVQDGLVTTERLRASGRAPAIIVLTTFDADEHVLRALAAGAGGFLLKDTPPAKLIAAIHAVAAGEPMLSPSVTRQLIAAATHAPGPDNGAASDLAQLTDRELEVAVEIGRGSSNAEIATRLFMSVATVKANVTRIFQKSGCTNRVALAMKVRDAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5W709</accession>
    <name>U5W709_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cellulose synthesis regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_32645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561125.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_32645"/>
    <dbReference type="EnsemblBacteria" id="AGZ44787">
        <property type="protein sequence ID" value="AGZ44787"/>
        <property type="gene ID" value="AFR_32645"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_32645"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6606"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022176_3_0_11"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="395"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="527" mass="57932" checksum="0AD7CA354E35381C" modified="2014-01-22" version="1">MTTEAAATAGAVVVDERAELEATLVELEGRPQSQFRAVAGPAATVERRATDLGDEALAMRARLLRATALVREGKSEQGGRWAHQVLDWSQQHDNPFLLSRAHRVLSIFYRQIGDLSEGLAHAVHCFANLTDDEPDTIRARHLMTLAVALDESGSIEEGDRRFREALALATAEGDLELTLNILNNMAYTAFENDNEPGALDLVEHMRAVQLRAGRDFGALELDTIARIEMMGGRYADVEQTLGFLVDGQSTVESHEGDASAVCLLTLAEARRLDRRYTAAQEALDASVRLAEDRGLDRVRAQAREEQAALYAATGRFHEAYEEHKAFYAAATALHSTQREAQARALQAVFEATEARRASEHFREMAHRDALTGLYNRRYVNERLPALLGEAVARRTPVSVAILDLDHFKRVNDTLSHSTGDTVLQEIAQLLLEAASGPMIAARMGGEEFLLVMPGLGIEEATERCERLRLRIRAHAWEPVTGALTVTTSIGVTTAPEGRATPSTLLSLADRNLYVAKREGRDRVIADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5W781</accession>
    <name>U5W781_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ornithine decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33705</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561336.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33705"/>
    <dbReference type="EnsemblBacteria" id="AGZ44999">
        <property type="protein sequence ID" value="AGZ44999"/>
        <property type="gene ID" value="AFR_33705"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33705"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6822"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_1_3_11"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="PTHR11482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="3">
        <location>
            <begin position="40"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="2">
        <location>
            <begin position="276"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="414" mass="44197" checksum="F6DE49214FE2826E" modified="2014-01-22" version="1">MTAALTGTRSQLPDQWPAALSPDCLEVIDMPTPYLVTDLDTVVRRLEAFTAALPGVLPFYAMKCNPAPEILATMRDHGAGFEIASLGELQMLQRQGVDPAGVLYSNPVKPPAHIAAAHAAGLWRFSFDSPNELRKLAEHAPGSAVYLRLRVDDSASSFPLSRKFGAEVHEARDLLVLARRLGLRPYGVTFHVGSQCGNAAAWREAIGAVGQIMADLLDEGIRLDLLDLGGGFPARYTEDVPTIGEIGEMVLPALDELLPYRPGLLVAEPGRHLVAEASVMAVSVIGREVRGTENWLFVDVGAYNGMMETIQLPTGWDFPMWTSVAGHGEIAELPFTITGPSCDSSDTMFYGALLPAGIDVGDIIYIGSTGAYTLSYASAFNGFPPPTPLFVGGPATLPRQTTGEPARAGIPGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W793</accession>
    <name>U5W793_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_27775</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560153.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_27775"/>
    <dbReference type="EnsemblBacteria" id="AGZ43816">
        <property type="protein sequence ID" value="AGZ43816"/>
        <property type="gene ID" value="AFR_27775"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_27775"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5629"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_16_1_11"/>
    <dbReference type="OrthoDB" id="50471at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02767">
        <property type="entry name" value="MopB_ydeP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010046">
        <property type="entry name" value="Mopterin_OxRdtse_a_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041953">
        <property type="entry name" value="YdeP_MopB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF4">
        <property type="entry name" value="PTHR43105:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01701">
        <property type="entry name" value="Fdhalpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin" evidence="3">
        <location>
            <begin position="141"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Molydop_binding" evidence="4">
        <location>
            <begin position="661"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ43816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="779" mass="85595" checksum="EEBB55331828E29C" modified="2014-01-22" version="1">MTEIPGDRQGQGAPGTPATGRGPNRGGDFSQRDDHHPSAGWGAARSVGKIIAAAREPVGAPHAVLKMNQLKLSAKKDGFDCPGCAWPDDRNGLHLDFCENGVKHVTWEMTRKKVDGEFFAKHTVTELNGWNDFDLENQGRITEPLSYDPASDKYVPISWDDAYALVGDTLRGLSGPDEASFYTSGRLSNEATFLYQLWVREFGTNNMPDCSNMCHEASGRALTAAIGTGKGTCDLEDWESADLLILMAVNAASNAPRMLTALAEASRRGAEIVHVNPLIEAASRRTIVPHDFVAMGTFQATKTGTMNVQPRIGGDLALLRGVAKVVFEQDAVDQHFIEQHTDQYEAYRKLVDDTPWDDVVHQSGVAEEIIRDLAGRYIASRRSIIAWCLGLTQQEHSVDTMREIINLLLLRGNIGRRGAGPSPIRGHSNVQGNRTCGVNHHPTEEWLAKHDAATGITSPREHGLGTVATIEAMAAGQVKVFVSLGGNFALAAPDTRATFAALRNTELTVQVSTKLNRSHLVHGRHALILPCLGRTELDVRATGEQGVTIEDSMSYVHLSYGKRKPSSPLLRSESAILCGLARATLPESATPWEEYAEDYDKIRDVMARALTGFEDFNRRVREPLGFRLDQPARERVWLTPSGKAEFHPSPLPDVVPPEGRLMLSTVRSHDQWNTTIYSDNDRYRGVKNLRTLLFMNREDMAERGLTEFDLIDITSHARDGSTRSVWGYRAIAYDTPRGSVTGYMPELNILCALTDNSSQSDQPLTKHLVVEVTPAAPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7C8</accession>
    <name>U5W7C8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561415.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45078">
        <property type="protein sequence ID" value="AGZ45078"/>
        <property type="gene ID" value="AFR_34100"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34100"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6900"/>
    <dbReference type="HOGENOM" id="CLU_123724_0_0_11"/>
    <dbReference type="OrthoDB" id="2076339at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="197" mass="21510" checksum="5B0FD9407A8584F9" modified="2014-01-22" version="1">MEPEVLVSAMAMVVSVLALGITSALTRAQVRSMRKANDLPVMIELLIKEYVNDEFQAQHRLVTETLPTVDASLGFEGLPEPLRTAAYRVAWYYNATGGLIALGTVDEIMFVGGINFRVRHAWSAMEAHIGVERNSRGQPFLDGFEHLAARCFANSPHVLEQTRRLEKVPATAQRVGFSLDPAQASAQPTTSSGSNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7I1</accession>
    <name>U5W7I1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_28250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560248.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28250"/>
    <dbReference type="EnsemblBacteria" id="AGZ43911">
        <property type="protein sequence ID" value="AGZ43911"/>
        <property type="gene ID" value="AFR_28250"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28250"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5726"/>
    <dbReference type="eggNOG" id="ENOG5033PW3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026219_0_0_11"/>
    <dbReference type="OrthoDB" id="1118327at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ43911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="472" mass="51643" checksum="6E7C07A569BCAC40" modified="2014-01-22" version="1">MTPPAYRDPLAVAIGNASLLGVGYFMLRRRRLAICTGMVTLILLLLLAGISRTRWLEIVVLSWWALMIVHGFFLARRGGAEEQWGRLIATARGNGAGRQRVTALALTVPVLVAFGLLRFEASRISETVADARRSGDCGEATAALDRVWLGLRVADAPSTLRGEKTVEACRRLRFAGDKLAEGLTGVTNDLDSGFDTLTSVLTELPGHERMVDTVLDGFLAGLPTPNPCLTLTITDWLTERPPSNNALDRSTAAVTRTAPAALVGCGDKKMSARDWQAARGNYQQLLKSYPGDALAGRARQGISKATVELELKTVRERLQGSDPEYCGKPAKYSAAPPYRKGTNRALFYGNDEYTDKLPGGWRAPDVTKAVLIVCAGETKFGKSVQSCPYENKKMPRFPTYVSFHKIAIPVKAYELRTGRLVANRTVQINGTSCPRILRYTTYLTDFGPPSQVYVKTTTAKVRSVFAPMIVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W7I5</accession>
    <name>U5W7I5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_28495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ43960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560297.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_28495"/>
    <dbReference type="EnsemblBacteria" id="AGZ43960">
        <property type="protein sequence ID" value="AGZ43960"/>
        <property type="gene ID" value="AFR_28495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_28495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5777"/>
    <dbReference type="HOGENOM" id="CLU_1718412_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ43960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="152" mass="16351" checksum="90776F7B15523A11" modified="2014-01-22" version="1">MAALLSLLGALGIIGSLLFAGWQARVLATQVGHQATRDGVATLQEALTSLREVQHYLVEDPSLIPHFAPERSRLPLEDADAGKVEMVAAMYCDVLNIGLYNLSAVSTARPESAWAQYCEHLLANSTAIRAEVLNKAWAYPRLSAMVGPQPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W869</accession>
    <name>U5W869_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_29275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023560453.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_29275"/>
    <dbReference type="EnsemblBacteria" id="AGZ44116">
        <property type="protein sequence ID" value="AGZ44116"/>
        <property type="gene ID" value="AFR_29275"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_29275"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.5932"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_11"/>
    <dbReference type="OrthoDB" id="1582456at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ44116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="282" mass="31151" checksum="9C8E3AEF09025FE9" modified="2014-01-22" version="1">MNKVLITGAGTGFGHEVALRLAEKGFDVIATVEIYGQIQTLKRQAHERGVKLQVEKLDVTNEGDRRKALGWGVEILVNNAGIGEGGSTVDIPAANIRNEWEVNVTGPLLLTQGIAKQMARRGAGRIVWVSSREGLNVNPFTGIYAASKHAVEAIAETMKLELQEFGVEVATVNPGPFLTGFNDRIFQTYHSWEDDPADRLFDYDKLAFPRAQYDPEPVYATMTAVAAGEIDTYRNLEPKEMIEETKNLIMAPWDKKTTDGLGTRPDQVQSSYDMEPETLITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5W878</accession>
    <name>U5W878_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_34780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561549.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_34780"/>
    <dbReference type="EnsemblBacteria" id="AGZ45212">
        <property type="protein sequence ID" value="AGZ45212"/>
        <property type="gene ID" value="AFR_34780"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_34780"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7037"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_11"/>
    <dbReference type="OrthoDB" id="1261526at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="24739" checksum="E25D289F31FAF709" modified="2014-01-22" version="1">MPVVIITGASSGIGRAAALRLGRPGTTLVLAARREAELAKVAAEIEAAGGTAVAVPTDVADPAQITALADRAGRVDVLINNAGVGGSPSVLTDDATVTAMVDVNLLAPIRLMRAVIPGMRAAGSGSIINIGSVAGEIGISGTYSATKFALRGMNDSVRRELAGTGIGVTLIEPGHIATELTAYRKGRMPGPEIVVDAIEKAIRRPRRRIVVPFRYRLAMAAASALPQVTDRSFAGKAARKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8L3</accession>
    <name>U5W8L3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)</fullName>
            <ecNumber evidence="4">1.17.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">ispG</name>
        <name evidence="5" type="ORF">AFR_36140</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:43604"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:10623"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IspG family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.3" evidence="4"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561820.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_36140"/>
    <dbReference type="EnsemblBacteria" id="AGZ45484">
        <property type="protein sequence ID" value="AGZ45484"/>
        <property type="gene ID" value="AFR_36140"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_36140"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7314"/>
    <dbReference type="eggNOG" id="COG0821">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042258_0_0_11"/>
    <dbReference type="OrthoDB" id="665306at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00096"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046429">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00159">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016425">
        <property type="entry name" value="IspG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004588">
        <property type="entry name" value="IspG_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30454">
        <property type="entry name" value="PTHR30454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04551">
        <property type="entry name" value="GcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004640">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00612">
        <property type="entry name" value="ispG_gcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="390" mass="40970" checksum="66AE5229A1CACF13" modified="2014-01-22" version="1">MTAISLGMPAVPPPPLATRRLSRQIMVGNVPVGGGAPVSVQSMTTTLTSDVNATLQQIAELTASGCQIVRVAVPSQDDVEALPAIARKSQIPVIADIHFQPKYVFAAIDAGCAAVRVNPGNIRQFDDKVKEIAAAASAANVPIRIGVNAGSLDKRLLAKYGKATAEALVESALWECSLFEEHGFRDIKISVKHNDPVVMIRAYRQLAEQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLAEGIGDTIRVSLSAPPVEEIKVGQGILESLGLRERGLEIVSCPSCGRAQVDVYTLAEQVTAALEGFPVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVKGQVIKTVPESIIVETLVEEALRLADSMGAELPEELRELLPGPTVTVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8N6</accession>
    <name>U5W8N6_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Argininosuccinate lyase</fullName>
            <shortName evidence="5">ASAL</shortName>
            <ecNumber evidence="3 5">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">argH</name>
        <name evidence="8" type="ORF">AFR_30205</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">DSM 7358</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="3 5"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30205"/>
    <dbReference type="EnsemblBacteria" id="AGZ44301">
        <property type="protein sequence ID" value="AGZ44301"/>
        <property type="gene ID" value="AFR_30205"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30205"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6116"/>
    <dbReference type="eggNOG" id="COG0165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_2_11"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="6">
        <location>
            <begin position="19"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="ASL_C2" evidence="7">
        <location>
            <begin position="370"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AGZ44301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="476" mass="51153" checksum="1090B605F3806A32" modified="2014-01-22" version="1">MPETEGPTRLWGGRFTGGPADALARLSVSVHFDWRLAPYDIAASRAHARVLAGAGLLDADELGQMLAALDDLESACAAGEFRPTVEDEDVHTALERGLLERLGGLGGKLRAGRSRNDQVATDLRMYLRDHARGVAAAVVELADALMEQAGRHIDTPAPGLTHVQHAQPVSFGHWLLAHVQPLLRDLDRLRDWDERTAISPLGSGALAGSSLPLDPGAVAKELGFKAPAQNSMDAVSDRDFVAEFLFITSLIGVHLSRLGEEVVLWTSQEFGWVILDDAFATGSSIMPQKKNADIAELARGKAGRLIGGLVAVLTMLKGLPLTYDRDMQEDKEPAFDAIDTLQLLLPALAGMISTMTVRVDRLAAAAPVGFSLATEVADWLVRKHVPFRDAHEITGRLVALCAARECELEDVSDEDLKTVSEHLDPAVRQVLSVKSALAARTTAGSTGPGPVADQLQTASDKLDGWREWSIEPVVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8Q3</accession>
    <name>U5W8Q3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribonucleoside-diphosphate reductase subunit beta</fullName>
            <ecNumber evidence="3">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">AFR_35680</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 5">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="3 5">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_35680"/>
    <dbReference type="EnsemblBacteria" id="AGZ45392">
        <property type="protein sequence ID" value="AGZ45392"/>
        <property type="gene ID" value="AFR_35680"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_35680"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7222"/>
    <dbReference type="eggNOG" id="COG0208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035339_2_1_11"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="PTHR23409"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000355">
        <property type="entry name" value="NrdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000355-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000355-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ45392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="325" mass="37397" checksum="EDB4A219AB22BC69" modified="2014-01-22" version="1">MDLTLRPMKYPHFFDRFKDAIKNTWTVEEVDLHSDLADLAKLSPAEQHLVSRLVAFFATGDTIVANNLVLNLYQHVNSPEGRLYLSRQLFEEAVHVQFYLNLLDTYVPDEAERAAAFDAVENIPSIARKADFCFRWIDSVFELRELRTKDERRAFLLNVICFAACIEGLFFYGAFAYVYYLRSRGLLHGLASGTNWVFRDESMHMAFAFDVVEQVRAEEPDLFDDKMEQQVRDMLSEAVECEVQFAADLLEQGVSGMSLTDMRTYLQHVADRRLTQLGIAPIYGSQNPFAFMELQDVQELSNFFERRVSAYQVGVTGSVSFDDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W8R8</accession>
    <name>U5W8R8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_30380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_30380"/>
    <dbReference type="EnsemblBacteria" id="AGZ44336">
        <property type="protein sequence ID" value="AGZ44336"/>
        <property type="gene ID" value="AFR_30380"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_30380"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6151"/>
    <dbReference type="eggNOG" id="COG0531">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_425603_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="485"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ44336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="702" mass="74309" checksum="8957AD2F79C087FE" modified="2014-01-22" version="1">MSAATSTPAGVRARGGRTVIEAVASAAAAAQKKDLMDTTEEVAEPEYVVPAAVDTPTRRQEPGGTLSRWLLRHQVQPVGPAAAQSDTEQHTWWKVMCLTGVDYFSSLAYVPAIAAAAAGAVSPLATLLIVGLTLLGMLPMYRRVAKESPHGAGSVAMLERLLPFWKGKIFVLILLGFVATSWIITITLSAADASLHVLENPHAPQFLEGHAVLITVILLLILGGVFLKGFSEAVGVAIPLVAVFLGLNAVVIIVGLTRIVTTPGAWSAWTTALTADGGGFSGLVVPAVLAFPLLVLGLSGFETGVAMMPLVAADGADAEQRLANRVRNTRKLLTAAAVIMSVYLLASSLVTTILIPAAEFAEGGAANGRALAWLAHEELGELFGTVYDVSTVLILWFAGASAMAGLVNIVPRYLPGYGMAPEWGRAVRPVVLVYTLISIIITIAFGADVDAQAGAYATGILAMMVSGAFAVTVSTARRHKRLPAIGFGLLTAVLFYALAENVIDKPDGIAISGLFIAGIIAVSLTSRVSRTTELRADRIEFDDDARRFITDTLAHDNRISLIARRRRDATDDAADYAAKEREQRGDNPVPGSADVLFLEIEVVDPSDFSEVLTVHGAEVGGHRVLRAEAPAAPNAIAAILLALRDSTGVRPHCYFAWAEGSPLVHMVRYFLLGRGDTAPVTREILRQYEPDPQRRPGIHVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9C6</accession>
    <name>U5W9C6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATP-dependent helicase HrpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_31550</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ44570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_31550"/>
    <dbReference type="EnsemblBacteria" id="AGZ44570">
        <property type="protein sequence ID" value="AGZ44570"/>
        <property type="gene ID" value="AFR_31550"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_31550"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6386"/>
    <dbReference type="eggNOG" id="COG1643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_5_6_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010225">
        <property type="entry name" value="HrpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013689">
        <property type="entry name" value="RNA_helicase_ATP-dep_HrpB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08482">
        <property type="entry name" value="HrpB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005496">
        <property type="entry name" value="ATP_hel_hrpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01970">
        <property type="entry name" value="DEAH_box_HrpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="21"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="208"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="798"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ44570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="821" mass="86928" checksum="CC4E353B9E7996B6" modified="2014-01-22" version="1">MLIAADALPDLPIRAVLTEVGDALAGAGAAVLVAPPGTGKTTLVPLALAGTGRVVVAEPRRVAARAAARRMAGLLGEQVGERVGYTVRGDRRVSAATRVEVVTTGVLVRRLQRDQELTGTTVVIIDECHERHLDTDLALAFLIEVRAALRPDLQVLATSATAESGRLSEILGDAPIIEAKAALFPVDVHWCPPPQPVAPPLGLRVDPKLLDHVATTVRRALTDGDGDVLVFLPGAREIETVASRLRGVDAEIVPLHGRQSGSIQDAALRPSPVRRVVLATAVAESSLTVPGVRAVVDAGLSRVPRMDHSRGLGALVTVPVSRAAATQRAGRAGREAPGRVYRCWSQAQHDRLPAQPEPEVAAADLTGFALDLALWGHPDGTGLALPDTPPAGAMQAAMGTLHALGATDTDGRITARGRALADAGLHPRLARALLDGAATVGARRAAEVVALLDGDRRADDLITAWRRARTDNDGAWRAEVRRLTAAITRNTSGGGSELPDDLAAGLIIGLAYPERVARAREAGGRAYLMAGGTAADLTGSSNLAGTPWLAVADADRAPGARTARIRSAAPLDEASACLAAATLRQTTTETGWIDGDVIARRLDRLGAITLSERRIERPDPTQVQAALRDGLQREGLALLTWSRTATELRERLAFAHAALGAPWPPVDDDALLSSLDDWLPLQQARRRADLAKIDTASALRRLLPWSVAGRLDDIAPERLPVPSGSKVRIDYTDPAAPVLAVKVQEAFGWKNAPRLADGRIPVLLHLLSPAGRPVAVTADLASFWANGYPQVRAELRGRYPRHPWPEDPTTAEPTRRTNRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9H3</accession>
    <name>U5W9H3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562134.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37725"/>
    <dbReference type="EnsemblBacteria" id="AGZ45799">
        <property type="protein sequence ID" value="AGZ45799"/>
        <property type="gene ID" value="AFR_37725"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37725"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7629"/>
    <dbReference type="HOGENOM" id="CLU_1998981_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025492">
        <property type="entry name" value="DUF4383"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14325">
        <property type="entry name" value="DUF4383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="124" mass="12382" checksum="B24F349EE75E28F2" modified="2014-01-22" version="1">MVTDTRRRVVERTRDLTGPAALGLGVALVIVGLAGCLAVGRGSLLGLLQVTLPHNMFHLAVGTALVSAAILGPRPARVTATTAGLTFLGLGVAGLAGAPGVTPNGADIAFYLAFGLALTAVGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5W9K8</accession>
    <name>U5W9K8_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4126 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562042.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37265"/>
    <dbReference type="EnsemblBacteria" id="AGZ45707">
        <property type="protein sequence ID" value="AGZ45707"/>
        <property type="gene ID" value="AFR_37265"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37265"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7539"/>
    <dbReference type="eggNOG" id="ENOG502Z9HI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086377_1_0_11"/>
    <dbReference type="OrthoDB" id="1828070at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025196">
        <property type="entry name" value="DUF4126"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13548">
        <property type="entry name" value="DUF4126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4126" evidence="2">
        <location>
            <begin position="5"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="208" mass="21974" checksum="9DF293315F585A1E" modified="2014-01-22" version="1">MLEALTGTGLAASAGLNAYIPLLTMGLLARYSDLIDLPSGWQWLSNGWVIGILALLLAVEVVADKVPVVDHVNDIVQTVVRPTAGGLAFGAGSSSETVTVTDPGSFFSSHQWVPIVIGMVLAFGVHTVKAASRPVVNATTGGVGAPVASTAEDFGSVVLSLLAIVLPVLVLVGLVLMVLATWWVIRRRKRRKRERQPLEGVETQRLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAL1</accession>
    <name>U5WAL1_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_38915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562371.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38915"/>
    <dbReference type="EnsemblBacteria" id="AGZ46037">
        <property type="protein sequence ID" value="AGZ46037"/>
        <property type="gene ID" value="AFR_38915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7875"/>
    <dbReference type="eggNOG" id="ENOG5030K6H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100956_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ46037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="241" mass="25629" checksum="FA37F2E4AECD1145" modified="2014-01-22" version="1">MTTVPVMSGAQAAAWHGVMDLHDRLDHGWTLVGGQLVHLHCAEQGHTPPRPTDDIDTVIDVRAAGDMLQTFTGVLTGLGFTSVGTSPEGYQHRWSRADAVIDVLLPEGVGERTAARPGVTGSPTLPTPGGTQALHRSVSVSVTVDGRTGSVRRPTLVGALVMKAAAHTTVGDAARGRHRFDFAALAALIAARDFRETELTKKDRSRLRAMLQATRADREVMLGLPDAPESLNRLERAAGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WAT9</accession>
    <name>U5WAT9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbohydrate binding family 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_33915</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561378.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_33915"/>
    <dbReference type="EnsemblBacteria" id="AGZ45041">
        <property type="protein sequence ID" value="AGZ45041"/>
        <property type="gene ID" value="AFR_33915"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_33915"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.6865"/>
    <dbReference type="eggNOG" id="COG2273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253733_0_0_11"/>
    <dbReference type="OrthoDB" id="1046649at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006584">
        <property type="entry name" value="Cellulose-bd_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03422">
        <property type="entry name" value="CBM_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00606">
        <property type="entry name" value="CBD_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM6" evidence="3">
        <location>
            <begin position="91"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ45041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="222" mass="23002" checksum="4C38B717564BE3A0" modified="2014-01-22" version="1">MTTPASPPPGVYHSRSRVTRNRLALAAAGLALVLLGYLIGRLQGGSDTPAIAAPPPAPVSASAAPATEPTAEPSTAPATTEPATPGKDAYAALQAEAASGQQGTDVQETEDEGGGQNVGWIAAGDWLRFDDVDFGDQPAKKFAARVASDANEGSNGRIEIRIDSPANAPVGTLSARGTGGWQDWASQETDIEPTKGRHTVFLVFAADNGDEFLNLNYFAFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WC54</accession>
    <name>U5WC54_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_42375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42375"/>
    <dbReference type="EnsemblBacteria" id="AGZ46729">
        <property type="protein sequence ID" value="AGZ46729"/>
        <property type="gene ID" value="AFR_42375"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42375"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8576"/>
    <dbReference type="eggNOG" id="ENOG5030HTH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346554_0_0_11"/>
    <dbReference type="OrthoDB" id="1992208at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="225" mass="23611" checksum="0FD5ACBE65EB9504" modified="2014-01-22" version="1">MFATMRRKNRSAKMKSELGQSVDHFKRAASIAAQETSATVGPRFNAAKDRVQPTAVKAKDAASNSWESALATLTPLVAAATDSARQTGKSTTKAGKKAVKSNKKNAKKLEKRTNKALGRKQKSRSGLKLGGLLLAGAAVGAAGAYVVRKRRREQWDEYDPSGPISSPVTGADDAAFEPADLSGPSGFVTPADETDSLVVDDGADQTSSTQHSPNVARLASGKNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCA4</accession>
    <name>U5WCA4_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">AFR_42675</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42675"/>
    <dbReference type="EnsemblBacteria" id="AGZ46789">
        <property type="protein sequence ID" value="AGZ46789"/>
        <property type="gene ID" value="AFR_42675"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42675"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8636"/>
    <dbReference type="eggNOG" id="COG1408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025443_5_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="3">
        <location>
            <begin position="369"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AGZ46789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="587" mass="60629" checksum="48AF69A63E778A32" modified="2014-01-22" version="1">MAFVGLIFVVIALIHFYLWKRLVRDTIRPGRLRKAGAWITVGLAILVPVTLIGVRAGYLMWLAWPGYLWVALMFYLLVVLVVLEIPRLVISLGIRVTSKRSAAAPATVAAGAKSGSVSATDAGSSTDAGLSAPAEAPAMPVAPDLETGRTASPDLATGRTAAPDLATGRTAAPDLATGRTAAPDLTTGPTAAPDLATGRTAAPDLTTGPTAAPDLATGRTAAPDLTTGPTAAPDLATGRTAAPDLTTGPTAAPDLATGRTAAPDLTTGPTAAPDLATGRTAAPHPDDSRPSASDRGEEQPTAAAQQGEPDNDQPGIDRRLLLARGAAIFAGLTAAGITGYGVKTALGPPQLDRVQLPLAKLPRAMDGTRLAVVSDIHLGPLTGPNHAARIVQLINSVNADVVCIVGDLVDGRVAELGKYAAPLADIQSRRGAYFVTGNHEYYSGFQEWVDEVARLNVRPLRNERLELDGMDLAGVNDLGGTEFDDGPDLQRALGDRDTTRPVVLMAHQPIIAQDAAPFGVDLQVSGHTHGGQMAPFNLLVKLEQPVVSGLGKVDGVPVYVTNGAGFWGPPVRVGAPPQVSVIELRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCD0</accession>
    <name>U5WCD0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_37005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023561991.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37005"/>
    <dbReference type="EnsemblBacteria" id="AGZ45655">
        <property type="protein sequence ID" value="AGZ45655"/>
        <property type="gene ID" value="AFR_37005"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37005"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7486"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108054_2_1_11"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="117" mass="13042" checksum="9FCC91C9FA5D02C0" modified="2014-01-22" version="1">MIGRLDTVVIDCPDPRALAGFYSELLGMHVVHDEDGWVTIRTDSDAPGLGFQKAPDLLPPDWPDRHRPQQFHLDVRVDNIELAEQRALAAGAKRLDGGGKDFRVYADPVGHPFCLVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCV3</accession>
    <name>U5WCV3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative Patatin-like phospholipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_37905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562170.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ45835">
        <property type="protein sequence ID" value="AGZ45835"/>
        <property type="gene ID" value="AFR_37905"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37905"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7665"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047251_4_0_11"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="7"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="2">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="38"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ45835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="274" mass="29491" checksum="661B2D103AA8601B" modified="2014-01-22" version="1">MDGPVAFVLGGGGVLGAVQVGMLRALFRAGFRPDVVVGTSIGAVNGALVAADPSEAVTERLVRLWSSPEAAEVYGDSLARQLRRFAARTHLHSPLPLRRLLTSELGENCEFSDLKVPFRCCAASIERAAEHWFDSGPLVDAVLASSAVPGLLPPMEVGGEHFVDGGIVNSIPIGEAVRVGAKQIFILQVGRIERPLVVPKRPWEVAQVAFEIARRHRFARDVASLPEDVRVHVLPTGGGESRDDSPWAYRDMAAAGRRISRAYTASRRYLAANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>U5WCY3</accession>
    <name>U5WCY3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TetR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_37710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562131.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_37710"/>
    <dbReference type="EnsemblBacteria" id="AGZ45796">
        <property type="protein sequence ID" value="AGZ45796"/>
        <property type="gene ID" value="AFR_37710"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_37710"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7626"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105089_0_0_11"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ45796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="186" mass="19891" checksum="778379FFDABD7845" modified="2014-01-22" version="1">MTDAGASARKLELLEAAYGYALEHGLGDLSLRPLAASIGSSPRVLLYLFGSKDGLVRALLARARTDEVGLLQQVGGEDLTQVARQVWEWLAAPGHRALLTLWTEAYARALVDPGGAWAGFARETVDDWLNLLAAAQPVSVRDTGEGVAERSHVLAVLRGALLDLLATGDVQRTTAAVERGISPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WD50</accession>
    <name>U5WD50_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Biotin/lipoyl attachment domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_38110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ45876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562211.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_38110"/>
    <dbReference type="EnsemblBacteria" id="AGZ45876">
        <property type="protein sequence ID" value="AGZ45876"/>
        <property type="gene ID" value="AFR_38110"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_38110"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7709"/>
    <dbReference type="eggNOG" id="COG4770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_9_1_11"/>
    <dbReference type="OrthoDB" id="1938042at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ45876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="71" mass="7496" checksum="CAFC4FB6E39A0A4F" modified="2014-01-22" version="1">MADEIRAEMVANVWKIVAAAGAEVEEGDTLVILESMKMEIPVLAESDGVVAQLAVNEGDVVQEGDLIAVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDL9</accession>
    <name>U5WDL9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562434.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39230"/>
    <dbReference type="EnsemblBacteria" id="AGZ46100">
        <property type="protein sequence ID" value="AGZ46100"/>
        <property type="gene ID" value="AFR_39230"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39230"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.7938"/>
    <dbReference type="eggNOG" id="COG3238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072587_0_0_11"/>
    <dbReference type="OrthoDB" id="1557785at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006750">
        <property type="entry name" value="YdcZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34821">
        <property type="entry name" value="PTHR34821"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04657">
        <property type="entry name" value="DMT_YdcZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="333" mass="33398" checksum="B1FF9EB3150B9744" modified="2014-01-22" version="1">MTRSSTTAPPAAPAVTAGRRAAGVLFALLAGIALAVQSRINGELSVRLDDPIGAATISFGSGLLVLAVLVPLLPVGRAGLRHLRAALREGRIRPWQCIGGMCGGFLVATQGLTVPTLGVAVFIVALVAGQSSSSLLVDRAGAGPAGPQPVTTPRLAGAVLTVCAVLLAVGDRIGHPSALALAVLPLVAGVGIAWQQAVNGHVRVVSGSAMVAGLINFIMGTAVLLVAFAVSVAIRGFPDRLPHGPWWLYVGGSLGIVFIAVGAAVVRYTGVLLLGLGMIAGQVTGALLLDEIVPGAAGRPGWNTVLGAALTLLAVGVAVVPARRRAGSPFRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5WDM5</accession>
    <name>U5WDM5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YadA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_39280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39280"/>
    <dbReference type="EnsemblBacteria" id="AGZ46110">
        <property type="protein sequence ID" value="AGZ46110"/>
        <property type="gene ID" value="AFR_39280"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39280"/>
    <dbReference type="eggNOG" id="ENOG5033FFJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_786729_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ46110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="35430" checksum="7A5EE90C162F0BC9" modified="2014-01-22" version="1">MAGWARGFADRCHQPRMSPTKPDFLDVGGVVGSVGPNFREVGGAVGSVGPDFVEVRPVIGLGGPDFLDVGGAASWAGPDFVDVGRVVGSVGPNFVEVGPEMSLAASDVLGLGRAVNPAGPDFLEVGPALHLGGPDFLEVGGAVGLVGPDFLEVGGAVSLASPDLLEIGRAVSSVVPNFVEVGPAMDLAGPDFLDVGGAASWAGPDFVDVGRALGSVGPNFVEVGPVMDLAGPNFLDVGGAASSASPDFLDVGRAVGSVGPNFVEVGPGMSLAASDVLGFGRAVNPAGPDFHEVGPEMHLGRPDFVEVRPAMSLAGPNFLEIAPAVTTTRPNFLEVGPSRNPAKPNFKEVGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>U5WE97</accession>
    <name>U5WE97_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative RNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_39985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023562584.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_39985"/>
    <dbReference type="EnsemblBacteria" id="AGZ46251">
        <property type="protein sequence ID" value="AGZ46251"/>
        <property type="gene ID" value="AFR_39985"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_39985"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8090"/>
    <dbReference type="eggNOG" id="COG0566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085947_0_0_11"/>
    <dbReference type="OrthoDB" id="1422015at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="2">
        <location>
            <begin position="72"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ46251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="220" mass="24444" checksum="AA13E54D51231A5C" modified="2014-01-22" version="1">MVDEIPGAVETTDEPVATDGIGVGPWPGEWPEDEHYDPELLAQGDRRNVVDRYRYWRREAVVADLDLRRHRFHVAIENWQHDFNIGTVVRNANAFLAAEVHIVGLRRWNRRGAMVTDRYQHVRHHPTIEDFTTWAAEAGLPVIGIDNLPGSRPMESVTLPRECVLIFGQEGPGLSEAARAACSALFSIAQYGSTRSINAGVASGIAMHSWVRAHGEPPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>U5WFK3</accession>
    <name>U5WFK3_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsF</name>
        <name evidence="6" type="ORF">AFR_42830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds together with S18 to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ46820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023563150.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_42830"/>
    <dbReference type="EnsemblBacteria" id="AGZ46820">
        <property type="protein sequence ID" value="AGZ46820"/>
        <property type="gene ID" value="AFR_42830"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_42830"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.8664"/>
    <dbReference type="eggNOG" id="COG0360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113441_5_3_11"/>
    <dbReference type="OrthoDB" id="1776954at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00473">
        <property type="entry name" value="bS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00360">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020814">
        <property type="entry name" value="Ribosomal_S6_plastid/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_S6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="PTHR21011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="SSF54995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AGZ46820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="96" mass="11022" checksum="ADA39916DF241228" modified="2014-01-22" version="1">MRHYELMVILDPSLEERTVAPSLDTYLNVIRTAGGSVEKLDVWGRRRLSFEINKKAEGIYAVVDLQATPEAVAELDRQLRLNESILRTKVIRPETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VMV7</accession>
    <name>U5VMV7_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DSBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357252.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00095"/>
    <dbReference type="EnsemblBacteria" id="AGZ38308">
        <property type="protein sequence ID" value="AGZ38308"/>
        <property type="gene ID" value="AFR_00095"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00095"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.20"/>
    <dbReference type="eggNOG" id="COG2761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069253_0_2_11"/>
    <dbReference type="OrthoDB" id="996965at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DSBA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="213" mass="22877" checksum="5887269E1CC3D9C1" modified="2014-01-22" version="1">MNIEVWSDVVCPWCYLGKRRLEAALQGFGSDVTMTFRAYQLDPSPVPQALPIKLAMAAKVGGPDRVNEMFDHVTTVAAGDGLVLDYDRAIAANTFDAHRLIAWAATQDLQLSMIEALQRAHFADGVDLGSHTELARLAGSIGLDEQAALAYLATPAGTDAVNADLAEARELGITSVPTFVVDRKFAIQGAQETAVLRSALDEIARREAVDAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNJ8</accession>
    <name>U5VNJ8_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00415"/>
    <dbReference type="EnsemblBacteria" id="AGZ38372">
        <property type="protein sequence ID" value="AGZ38372"/>
        <property type="gene ID" value="AFR_00415"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00415"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.85"/>
    <dbReference type="eggNOG" id="COG0503">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063339_3_3_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="182" mass="19093" checksum="0389D4C159E77422" modified="2014-01-22" version="1">MASDLRDRLRAGFRWTDPGPGTDLLVSDRSGWWRDPLVLDGLGPGLAGLFAGSQPTVVVSPEVTGFLIGPLVARALGTGFVEAYRAGARRPIAEPMTWAAVPSDHRGDQQHLGVRTHLVGPADRVLIVDDWAATGAQARGLTSLLGLLGAQVIGTAVIVDECNPAISAELKIRGLLTGDDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VNY5</accession>
    <name>U5VNY5_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_01820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357596.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01820"/>
    <dbReference type="EnsemblBacteria" id="AGZ38653">
        <property type="protein sequence ID" value="AGZ38653"/>
        <property type="gene ID" value="AFR_01820"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01820"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.368"/>
    <dbReference type="eggNOG" id="COG3177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058793_1_0_11"/>
    <dbReference type="OrthoDB" id="1374721at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="247" mass="25621" checksum="9FC29DF8704DEB60" modified="2014-01-22" version="1">MTSDPLAPLLALADVEPAFTQAREHVDAAMRHRALRRNGGQVAAEVSLRAAVASAALEDHRYDLDEVRAGTVTDPVLQGALRVAEGVGGLVDLWPRAPRQVLARLHVLAARGVVPDDDLGRLTSAADRIDALAGLVAGNDRTPPLLLAAIVHAELITLKPFAGPAGLVARAAARLTLIGRGFDPRGIVGVEAGHLAREPEYTGSAGAYATGTPDGVRSWLRHYASAVSAGADEIAQIGDDVLTPSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2020-08-12" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP27</accession>
    <name>U5VP27_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_02120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02120"/>
    <dbReference type="EnsemblBacteria" id="AGZ38713">
        <property type="protein sequence ID" value="AGZ38713"/>
        <property type="gene ID" value="AFR_02120"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02120"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.429"/>
    <dbReference type="eggNOG" id="ENOG502ZBU7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035969_1_1_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021391">
        <property type="entry name" value="DUF3027"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11228">
        <property type="entry name" value="DUF3027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="267" mass="28487" checksum="45989906F861339F" modified="2014-01-22" version="1">MRAARLDQVCADAVGIARGAITEVDPSDIGEHLEAVAEGDRVVTHFFESHLAGYRGWRWAVTVTRVPRSRHVTICETVLLPGPDAMMAPAWLPWNERVQAGDLGVGDLMPTAPDDDRLAPGYVLSDDPAVEDVSWELGLGRSRVMSQEGRIETAQRWYDGDSGPEAPIATAAPRNARCGTCGFYLPLAGSMRSMFGVCGNLYAPDDARAVSADHGCGAHSEALLGTTEQPVEELPTVYDDSEVEAVAVSRAPGSVESGEPAEPYGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VP77</accession>
    <name>U5VP77_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallophos domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_01500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357532.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_01500"/>
    <dbReference type="EnsemblBacteria" id="AGZ38589">
        <property type="protein sequence ID" value="AGZ38589"/>
        <property type="gene ID" value="AFR_01500"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_01500"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.301"/>
    <dbReference type="eggNOG" id="COG0207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_376264_0_0_11"/>
    <dbReference type="OrthoDB" id="1749560at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="4"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="737" mass="82390" checksum="3A1EA3594F0725DC" modified="2014-01-22" version="1">MSLILHLSDLHLSPPDDRETVGDHKINVIPLQDRVRRTELIRTTLRELGRALASGRRPLDAVVISGDVTYQGRADGFDLLPRTLEELGPVLPDPDKILVVPGNHDVRWYSAPSSAERYEQFLRLRTLGYRTPLLEGIDFNRYGEMPSAPPHPPTVVAEDGSFVLVGLNTANHCGVEMETTPEVRAAYAALDSRAGTDPDLRTLLDDWKLRGRFDIARLGPHQQRHASDALRELAPEGAVRIAVMHHQLLPISPDEEVKPFEALTNLGEVRDFLAGNNIDLLLHGHKHVEHIYADRYRPSLRGLNNGIRKLLVCSAGTVGLGQAYGGEVAKLLTIDGKHLAARRVTVESVPATRNGIPLMVSAFRTESYRIANDEPAETGEITGTTAQDVHEQLIDLYAKDRIQPRSPLICRFTDGQSALNRPASYPPLPLGRANDDDWFERMTGLWQRHTPIRGMPFNHGSRIYNYGGNRDQLDAAAQTLSRDPASGKALVVLLEPMRDHPDGTDLRYPAFCLAQFVVDGDRLDVVAYFRKQEMRYWWAINVAELAILQERMLEELRALDAQYEPGEITTVTAIPVVAEKVPRVAVPRIDQVADEQPGELVRLALSVCARDFPDRDTFVDQWSAVVNDWRPGLTATVDGDPVATFGLQALAEVIENVSHAIGTEDRVTEIISCLKQMHQQNVSFATKMQSRNNYKRTHEEWRREVQPLVTRLLATVNRILKSPDHPHLVQGPQDRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPI7</accession>
    <name>U5VPI7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AGZ38714">
        <property type="protein sequence ID" value="AGZ38714"/>
        <property type="gene ID" value="AFR_02125"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02125"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.430"/>
    <dbReference type="eggNOG" id="ENOG5033IM2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150710_2_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019681">
        <property type="entry name" value="DUF2530"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10745">
        <property type="entry name" value="DUF2530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="111" mass="11988" checksum="237B6D92BD9B7555" modified="2014-01-22" version="1">MVVADLSPAPHDEHLREQDHHLHGLDAVTGTKPRPEPLDPPMVPFALGGIGAFAVAGVVLLLAGAPERWLWTCLAGLLCGIPGLLTMLRHDANRRRRRALSHPDFKVTGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>U5VPV9</accession>
    <name>U5VPV9_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">AFR_02750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02750"/>
    <dbReference type="EnsemblBacteria" id="AGZ38839">
        <property type="protein sequence ID" value="AGZ38839"/>
        <property type="gene ID" value="AFR_02750"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02750"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_2_2_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AGZ38839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="179" mass="19519" checksum="2124A833F837B699" modified="2014-01-22" version="1">MDAEQLGAYLALKDVSSLLEHAVEQQLRVDGGLSGPQFQILARLSAAPERRLRMTDVADSMVYSRSGLTYQAGQLEKAGLIKRTPGEDDERSIIVTLTPAGQARLDQVLPGHIEVVQSLLLTSLSRRDITTITEILGRVRDRMRTAPPRSAAPRSTATRSTVSRSTAASRRKAGERDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ44</accession>
    <name>U5VQ44_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">L-rhamnose isomerase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03240"/>
    <dbReference type="EnsemblBacteria" id="AGZ38937">
        <property type="protein sequence ID" value="AGZ38937"/>
        <property type="gene ID" value="AFR_03240"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03240"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.652"/>
    <dbReference type="eggNOG" id="COG4952">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059875_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013457">
        <property type="entry name" value="Rhamnose_iso-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02635">
        <property type="entry name" value="RhaI_grampos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="55"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="388" mass="42605" checksum="BCE0FC5C741DF80B" modified="2014-01-22" version="1">MADLAAVKRALAAQRIETPSWAFGNSGTRFKVFAQAGVPRTPEEKIADAATVHKYTGIAPTVALHIPWDKVDDYAALGEYAASVGVGIGAINTNVFQDDDYKLGSVTNPDPGIRRKATDHLLEAVDIMDATGSQDLKLWFSDGINYPGQDDLRNRQDRLAAALRETYDRLGDNQRILLEYKLFEPAFYATDIPDWGTSYAHCIELGEKATVCIDTGHHAPGTNIEFIVAFLLRQKKLGAFDFNSRFYADDDLMAGAADPFQLFRIMYEIVRGGALDPAYGINFMLDQCHNIEAKIPAIIRSVMNIQEATAKALLVDREALTKAQNDGDVLEANAVIMDAYNTDVRPLLGEVRADAGLDPNPIKAYRESGYQEQIVKDRVGGEQAGWGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VQ88</accession>
    <name>U5VQ88_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Smr protein/MutS2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_03970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358028.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_03970"/>
    <dbReference type="EnsemblBacteria" id="AGZ39083">
        <property type="protein sequence ID" value="AGZ39083"/>
        <property type="gene ID" value="AFR_03970"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_03970"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.802"/>
    <dbReference type="eggNOG" id="COG2840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2467710_0_0_11"/>
    <dbReference type="OrthoDB" id="1888882at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SSF160443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="1">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="94" mass="10874" checksum="D7A011CF5E6742B3" modified="2014-01-22" version="1">MKLKLDLHDIFNKGTEIDKALRGIIDEAIQKKATLVEIIPGKGSGALKKKVLRFLDQREIKQLYHRVEKDGDNWGRLFIHFKFESSQGKGRRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRH0</accession>
    <name>U5VRH0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polycystic kidney disease protein 1-like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_00180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00180"/>
    <dbReference type="EnsemblBacteria" id="AGZ38325">
        <property type="protein sequence ID" value="AGZ38325"/>
        <property type="gene ID" value="AFR_00180"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00180"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.37"/>
    <dbReference type="HOGENOM" id="CLU_743212_0_0_11"/>
    <dbReference type="OrthoDB" id="2176128at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="352" mass="36057" checksum="54F830FBA329769B" modified="2014-01-22" version="1">MTGSGVERFQRTAFLPSSVPSAGVSALMPPHSQGAERRREHRPGRTGSRWALRTLVIGGLAGAAWLLTGAAAHAADRDPAGEGLSLGSSLIGSVVHGDDRSGPVVNRVLKAATTPLDPGHRGLGSAVALPNMVVGTLHEATHSKVTHSKSDADSALGGVDGVVRELTAPLRPAGEAVDTRKLAPVTELPQDEVLVDDVAAPEEPAKQAVAQVDDTRAAVADETVADQVSSAVVRDEELPAAEPVLDRRNTVGKTVTRQHSLAADRHRAAVVEAEPVMVSDTPGGDAPAPLRVHLGAANGVPASGPGTATDGGSAAFLPAKVADSTVAFHRLPIATDVEVRRHDAEAPTVSPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRI0</accession>
    <name>U5VRI0_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">AFR_05690</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">DSM 7358</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358958.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05690"/>
    <dbReference type="EnsemblBacteria" id="AGZ39427">
        <property type="protein sequence ID" value="AGZ39427"/>
        <property type="gene ID" value="AFR_05690"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05690"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1154"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_5_1_11"/>
    <dbReference type="OrthoDB" id="384611at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="4">
        <location>
            <begin position="17"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AGZ39427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="478" mass="49862" checksum="FE5B9BB13D9DD499" modified="2014-01-22" version="1">MDTTAFYVAGEAVHGDEEVAVTHPYDGRVVGRTTWARDEQVEAAVAAAAAVADRAAALPAHVRAAALDHVSRRIGERREEIAALITAENGKPLKWARIEADRAVSVFRWAAEEARRFAGDLQRLDTDPGGTGRIAVVRRVPRGPVLGISPFNFPLNLVAHKVAPAIAVGAPIVLKPAPATPLTALLLGEILAETDLPAGMFSVLPVPNDRAPALVADPRLPIVSFTGSGPVGAQIRRAAPEKHVTLELGGNAAVLLCADYGSDADLDWAAQRVATFGTYQAGQSCIAVQRVYVHAAHTGFLPRLTEAVAALRTGDPNDPEVEVGPMISEDAAKRVEQWVDEAVAAGATVVAGGKRDGATYPPTVLTGVPAGAKVVAEEVFGPVLVVSEVTDDTAGFAAINNSAYGLQAGVFTHSIQTAFTAHRELKVGGVIVGDVPSYRADQMPYGGVKGSGVGREGVASAMDDYTEPRVMVLTGVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRL0</accession>
    <name>U5VRL0_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide chain release factor 2</fullName>
            <shortName evidence="4">RF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">prfB</name>
        <name evidence="7" type="ORF">AFR_05840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">DSM 7358</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="4">Methylated by PrmC. Methylation increases the termination efficiency of RF2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023358988.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_05840"/>
    <dbReference type="EnsemblBacteria" id="AGZ39457">
        <property type="protein sequence ID" value="AGZ39457"/>
        <property type="gene ID" value="AFR_05840"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_05840"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1186"/>
    <dbReference type="eggNOG" id="COG1186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_6_0_11"/>
    <dbReference type="OrthoDB" id="928964at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00094">
        <property type="entry name" value="Rel_fac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004374">
        <property type="entry name" value="PrfB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00020">
        <property type="entry name" value="prfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0488">Methylation</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="6">
        <location>
            <begin position="244"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="69"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="4">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AGZ39457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="371" mass="41504" checksum="FE4D3A3F3B5B7A29" modified="2014-01-22" version="1">MSAADFPEQLKALDATLRNIENVLDVDKLRRDRAVLEEQASAPDLWDDQARAQEVNSRLSYLTGEIHRMEKLRQRIDDAELLLEMAQDADDEGSIAEVGDELVSLTKAVEEMEVRTLLSGEYDSREAVVAIRAGAGGVDAADFAEMLMRMYLRWAERHGYPTEVYETSYAEEAGLKSATFAVKVPYAYGTLSVESGTHRLVRISPFDNQGRRQTSFAGVEVMPVVEQTDHIDIPDNELRVDVYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKASAMRVLQARLLERKRQEEEAKMAGLKQDTTGSWGDQMRSYVLHPYQMVKDLRTEQETGNPSSVFDGELDEFIEAGIRWRKQTQIAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRN3</accession>
    <name>U5VRN3_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_00505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357334.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_00505"/>
    <dbReference type="EnsemblBacteria" id="AGZ38390">
        <property type="protein sequence ID" value="AGZ38390"/>
        <property type="gene ID" value="AFR_00505"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_00505"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.103"/>
    <dbReference type="eggNOG" id="COG3057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_679507_0_0_11"/>
    <dbReference type="OrthoDB" id="649993at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ38390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="407" mass="44090" checksum="B34E2D2E0E4C0C54" modified="2014-01-22" version="1">MAAPQTPYDAVLHAARDVAKLDCALDAEMLGTALLGSVYSVAATDRATAVREFVGQFLSATTRRRTASATTIREVFAALVPDAEGAQEVRRGPQAPVWTEQLGKVRPTGCYAYGDVYGDQTSYLVTFAYDNTDDGGPEHAVVALVDHNIGITKDVFVGGPAERILGQVREMCATDELTWFREEDAGRLRGEVSKHLEITDGLSDLPADGNLATDRALATARLALLPASTVPQLTEPPELSGPDRERLLRDFLSSPEAARFTLNDVSADDELASLHFCLSLILDHAASLPEPDPLRWSPAVAGLFLLDWVHRRAVLDMDDAAMLPRVTRAWSAYAIRKRGLPAAAAEQTDTVVEEMIPEFARLYATGEKRSPATAAVAQLISEGVDPNDAEAIDAWIETNRHRLTDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VRW6</accession>
    <name>U5VRW6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">AFR_07145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07145"/>
    <dbReference type="EnsemblBacteria" id="AGZ39718">
        <property type="protein sequence ID" value="AGZ39718"/>
        <property type="gene ID" value="AFR_07145"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07145"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1458"/>
    <dbReference type="eggNOG" id="COG3897">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074455_1_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AGZ39718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="209" mass="21927" checksum="D32792C5F65EC879" modified="2014-01-22" version="1">MSTSLLPVPLAPEISLHLADDKVGLLDGTGGEFSTDVPLPFWLFVWAGGQALARYVLDRPELVRGRTVVDVASGSGVAAIAAAKAGASAVTALDVDPRAVEAAVRNAAANDLVVEAHAGDIATADITADVFLAGDVYYTARVADRMTARLRRANREGARVLVGDPGRGFFPTKLFTQVAEYVVPVPPALEETGSLVTGVWEMRTTITAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS28</accession>
    <name>U5VS28_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07495"/>
    <dbReference type="EnsemblBacteria" id="AGZ39788">
        <property type="protein sequence ID" value="AGZ39788"/>
        <property type="gene ID" value="AFR_07495"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07495"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1530"/>
    <dbReference type="eggNOG" id="ENOG502ZH8Y">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163307_0_0_11"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="150" mass="15278" checksum="C90635F834F5DE04" modified="2014-01-22" version="1">MRPPSSSATPPAAPADPATVAPGPPVSATPAAEAESAQAETSAVLDPVEEAPKPAARPPRAVVTGRPARPANTGRPVSRPTSGAGAGGDDQSERGLRGLVGSGSSQVSVGAALRARDAARPTEQQLAEADTELVIVRRNWMPREDLPRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>U5VS59</accession>
    <name>U5VS59_9ACTN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359220.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07000"/>
    <dbReference type="EnsemblBacteria" id="AGZ39689">
        <property type="protein sequence ID" value="AGZ39689"/>
        <property type="gene ID" value="AFR_07000"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07000"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1428"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018489_2_0_11"/>
    <dbReference type="OrthoDB" id="514542at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="365"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ39689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="566" mass="57888" checksum="5098D0AD2D22D489" modified="2014-01-22" version="1">MAIVVVLAGSWLGYQQLADGGCSGQIKLTVAAATEIAPAVEQAAQQWTTDGANVNGTCVAVNVTGVNPSTTAAAVAAKHGVSLTGLGSGGKSAVLPDVWIADSSSWLLRLRSEASGFVPTDGKSIAQSPVVVAMPEPVAQQVGWPDKKLAWSDLVKKMTTGNSLRTGIVDPTRDAAGLAGLLALGSSVGTDAKAKATQVGALRALAAGSSSLRDDLLQKFPRSLDAADLASAVSAAPLSEEDVIAYNAERPQVKLAALYLDPVPPPLDYPFAVMPEVDLTKSAAATGLREALQQPSFKNQLAAVGLRAPDGTVGAGFAGPVGAPQASPAAQAPGGGSEGAAAAGLDARFINQALGSWAAITLPGRALAVFDVSGSMLDKVPTAGGASRAAVTRGAASQGLALFDDKWAVGVWIFSTELVGKRPWKQIVPISPLTSSRAQLQDSISQIVPKPKGATGLYDTALAAYKNVQDTWQAGRVNSVLLFTDGQDENPDGISQDKLVAELKRVQDPKRPVRMVIIGIGPGVDRNELETITNATSSGGVFIAPDPAKISDIFLEAISSRSGAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSK7</accession>
    <name>U5VSK7_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_02030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ38695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023357638.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_02030"/>
    <dbReference type="EnsemblBacteria" id="AGZ38695">
        <property type="protein sequence ID" value="AGZ38695"/>
        <property type="gene ID" value="AFR_02030"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_02030"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.411"/>
    <dbReference type="eggNOG" id="ENOG5030MT8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147818_0_0_11"/>
    <dbReference type="OrthoDB" id="1840390at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045629">
        <property type="entry name" value="DUF6232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19744">
        <property type="entry name" value="DUF6232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AGZ38695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000017746"/>
        </source>
    </evidence>
    <sequence length="147" mass="16156" checksum="5C994D54B4B3EAB2" modified="2014-01-22" version="1">MRTYYRGPDAVVTSDIFAGSSAPTRTFAIHDLRNVCITRTDVENFRPGALHVSAGLLLIAVAAWPLWKASPLLALGLVAMGMPAFIAGAVFWRLRPQRWELRATYRGADVALFTSSDERVFNQVSRALRRAIEDAGPPTAWENRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-01-22" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>U5VSN6</accession>
    <name>U5VSN6_9ACTN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">AFR_07950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Actinoplanes friuliensis DSM 7358</name>
        <dbReference type="NCBI Taxonomy" id="1246995"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Actinobacteria</taxon>
            <taxon>Micromonosporales</taxon>
            <taxon>Micromonosporaceae</taxon>
            <taxon>Actinoplanes</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2014" name="J. Biotechnol." volume="178" first="41" last="42">
            <title>Complete genome sequence of the actinobacterium Actinoplanes friuliensis HAG 010964, producer of the lipopeptide antibiotic friulimycin.</title>
            <authorList>
                <person name="Ruckert C."/>
                <person name="Szczepanowski R."/>
                <person name="Albersmeier A."/>
                <person name="Goesmann A."/>
                <person name="Fischer N."/>
                <person name="Steinkamper A."/>
                <person name="Puhler A."/>
                <person name="Biener R."/>
                <person name="Schwartz D."/>
                <person name="Kalinowski J."/>
            </authorList>
            <dbReference type="PubMed" id="24637369"/>
            <dbReference type="DOI" id="10.1016/j.jbiotec.2014.03.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">DSM 7358</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP006272">
        <property type="protein sequence ID" value="AGZ39879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_023359410.1">
        <property type="nucleotide sequence ID" value="NC_022657.1"/>
    </dbReference>
    <dbReference type="STRING" id="1246995.AFR_07950"/>
    <dbReference type="EnsemblBacteria" id="AGZ39879">
        <property type="protein sequence ID" value="AGZ39879"/>
        <property type="gene ID" value="AFR_07950"/>
    </dbReference>
    <dbReference type="KEGG" id="afs:AFR_07950"/>
    <dbReference type="PATRIC" id="fig|1246995.3.peg.1623"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_2_0_11"/>
    <dbReference type="OrthoDB" id="537440at2"/>
    <dbReference type="Proteomes" id="UP000017746">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/
[file cut: first 33554432 bytes kept]
[document cut: first 30661182 of 33541772 characters kept]
